DYSF gene cDNA ORF clone, Macaca mulatta(Rhesus monkey)

mRNA	Protein	Name
XM_015112636.1	XP_014968122.1	dysferlin isoform X12
XM_015112637.1	XP_014968123.1	dysferlin isoform X13
XM_028832388.1	XP_028688221.1	dysferlin isoform X1
XM_028832392.1	XP_028688225.1	dysferlin isoform X5
XM_028832393.1	XP_028688226.1	dysferlin isoform X6
XM_028832394.1	XP_028688227.1	dysferlin isoform X7
XM_028832400.1	XP_028688233.1	dysferlin isoform X13
XM_028832406.1	XP_028688239.1	dysferlin isoform X19
XM_015112626.1	XP_014968112.1	dysferlin isoform X2
XM_015112629.1	XP_014968115.1	dysferlin isoform X5
XM_028832402.1	XP_028688235.1	dysferlin isoform X15
XM_028832403.1	XP_028688236.1	dysferlin isoform X16
XM_028832409.1	XP_028688242.1	dysferlin isoform X21
XM_028832410.1	XP_028688243.1	dysferlin isoform X22
XM_015112628.1	XP_014968114.1	dysferlin isoform X4
XM_015112631.1	XP_014968117.1	dysferlin isoform X7
XM_015112632.1	XP_014968118.1	dysferlin isoform X8
XM_015112635.1	XP_014968121.1	dysferlin isoform X11
XM_015112639.1	XP_014968125.1	dysferlin isoform X15
XM_028832389.1	XP_028688222.1	dysferlin isoform X2
XM_028832390.1	XP_028688223.1	dysferlin isoform X3
XM_028832391.1	XP_028688224.1	dysferlin isoform X4
XM_028832397.1	XP_028688230.1	dysferlin isoform X10
XM_028832399.1	XP_028688232.1	dysferlin isoform X12
XM_028832404.1	XP_028688237.1	dysferlin isoform X17
XM_015112627.1	XP_014968113.1	dysferlin isoform X3
XM_015112633.1	XP_014968119.1	dysferlin isoform X9
XM_028832395.1	XP_028688228.1	dysferlin isoform X8
XM_028832396.1	XP_028688229.1	dysferlin isoform X9
XM_028832398.1	XP_028688231.1	dysferlin isoform X11
XM_028832401.1	XP_028688234.1	dysferlin isoform X14
XM_028832405.1	XP_028688238.1	dysferlin isoform X18
XM_028832407.1	XP_028688240.1	dysferlin isoform X20
XM_015112625.1	XP_014968111.1	dysferlin isoform X1
XM_015112630.1	XP_014968116.1	dysferlin isoform X6
XM_015112634.1	XP_014968120.1	dysferlin isoform X10
XM_015112638.1	XP_014968124.1	dysferlin isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMb45700	XM_015112625.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45701	XM_015112626.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45702	XM_015112627.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45703	XM_015112628.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45704	XM_015112629.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45705	XM_015112630.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45706	XM_015112631.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45707	XM_015112632.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45708	XM_015112633.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45709	XM_015112634.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45710	XM_015112635.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45711	XM_015112636.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45712	XM_015112637.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45713	XM_015112638.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb45714	XM_015112639.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OMb68132	XM_028832388.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68133	XM_028832389.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68134	XM_028832390.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68135	XM_028832391.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68136	XM_028832392.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68137	XM_028832393.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68138	XM_028832394.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68139	XM_028832395.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68140	XM_028832396.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68141	XM_028832397.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68142	XM_028832398.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68143	XM_028832399.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68144	XM_028832400.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68145	XM_028832401.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68146	XM_028832402.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68147	XM_028832403.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68148	XM_028832404.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68149	XM_028832405.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68150	XM_028832406.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68151	XM_028832407.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68152	XM_028832409.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMb68153	XM_028832410.1 Latest version!	Macaca mulatta dysferlin (DYSF), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

DYSF ( XM_015112625.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968111.1 Macaca mulatta dysferlin (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMb45700
Clone ID Related Accession (Same CDS sequence)	XM_015112625.1
Accession Version	XM_015112625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCAGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGATGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTCCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACT CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968111.1
CDS	189..6632
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112625.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112625.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCAGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGATGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTCCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACT CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112626.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968112.1 Macaca mulatta dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OMb45701
Clone ID Related Accession (Same CDS sequence)	XM_015112626.1
Accession Version	XM_015112626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCAGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GATGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCACGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACTCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC ACGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT CTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC AAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCTTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCAGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTTATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968112.1
CDS	187..6546
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112626.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112626.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCAGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GATGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCACGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACTCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC ACGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT CTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC AAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCTTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCAGGGATGA GCCCAACATG AACCCTAAGC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTTATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112627.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968113.1 Macaca mulatta dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OMb45702
Clone ID Related Accession (Same CDS sequence)	XM_015112627.1
Accession Version	XM_015112627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCAGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGATGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACTCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGGT CACTCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCGC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CGTGGAGGAG 
CGTCTGGCTC TGCACGTGCT TCAGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTC TATTATCTGG AATACAAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACA AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CAGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTTATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968113.1
CDS	186..6524
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112627.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112627.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCAGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGATGCCGG TCACCTGAGC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGCAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCAC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAACCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACTCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCA CGCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGGT CACTCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCGC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CGTGGAGGAG 
CGTCTGGCTC TGCACGTGCT TCAGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTC TATTATCTGG AATACAAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACA AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CAGGGATGAG 
CCCAACATGA ACCCTAAGCT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTTATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112628.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968114.1 Macaca mulatta dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OMb45703
Clone ID Related Accession (Same CDS sequence)	XM_015112628.1
Accession Version	XM_015112628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCAGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GATGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCACGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACTCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968114.1
CDS	186..6482
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112628.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112628.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTTCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACG TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCAGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GATGCCGGTC ACCTGAGCTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GCAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCACGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AACCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACTCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112629.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968115.1 Macaca mulatta dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OMb45704
Clone ID Related Accession (Same CDS sequence)	XM_015112629.1
Accession Version	XM_015112629.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTT 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACGTAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CAGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAT 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCACGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968115.1
CDS	298..6654
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112629.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112629.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTT 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACGTAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CAGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAT 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCACGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCACG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACAAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAGCTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112630.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968116.1 Macaca mulatta dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OMb45705
Clone ID Related Accession (Same CDS sequence)	XM_015112630.1
Accession Version	XM_015112630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTT 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACGTAG AAGAGGAGAC TGCAGGTGCC GTCAAGCCTT CGAAAGTCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCAGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT TCTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAAGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ATGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCACGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGATA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GTACTCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCACGC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGGTCAC TCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCGCAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCGT GGAGGAGCGT 
CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG CCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTCTAT TATCTGGAAT ACAAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACCGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACAAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCAG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTTATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968116.1
CDS	298..6633
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112630.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112630.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTT 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACGTAG AAGAGGAGAC TGCAGGTGCC GTCAAGCCTT CGAAAGTCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCAGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT TCTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAAGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ATGCCGGTCA CCTGAGCTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCACGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA ACCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGATA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GTACTCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCACGC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGGTCAC TCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCGCAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCGT GGAGGAGCGT 
CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG CCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTCTAT TATCTGGAAT ACAAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACCGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACAAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCAG GGATGAGCCC 
AACATGAACC CTAAGCTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTTATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112631.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968117.1 Macaca mulatta dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OMb45706
Clone ID Related Accession (Same CDS sequence)	XM_015112631.1
Accession Version	XM_015112631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCAGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ATGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCACGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACTCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCA CGCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACTCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCGC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CGTGGAGGAG CGTCTGGCTC TGCACGTGCT TCAGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTC TATTATCTGG 
AATACAAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACA AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CAGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTTATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968117.1
CDS	186..6494
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112631.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112631.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCAGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ATGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCACGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACTCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCA CGCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACTCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCGC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CGTGGAGGAG CGTCTGGCTC TGCACGTGCT TCAGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTC TATTATCTGG 
AATACAAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACA AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CAGGGATGAG CCCAACATGA ACCCTAAGCT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTTATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112632.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968118.1 Macaca mulatta dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OMb45707
Clone ID Related Accession (Same CDS sequence)	XM_015112632.1
Accession Version	XM_015112632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC AGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGATG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCACGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CTCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968118.1
CDS	298..6603
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112632.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112632.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC AGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGATG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCACGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CTCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112633.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968119.1 Macaca mulatta dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OMb45708
Clone ID Related Accession (Same CDS sequence)	XM_015112633.1
Accession Version	XM_015112633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTT 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACGTAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CAGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAT 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCACGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCACGCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGGTCACTC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCGCAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGCC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTCTATTA TCTGGAATAC AAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACCGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACAAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCAGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT TATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968119.1
CDS	298..6591
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112633.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112633.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTT 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACGTAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CAGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAT 
GCCGGTCACC TGAGCTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGCA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCACGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAAC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCACGCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGGTCACTC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCGCAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGCC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTCTATTA TCTGGAATAC AAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACCGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACAAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCAGGG ATGAGCCCAA CATGAACCCT AAGCTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT TATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112634.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968120.1 Macaca mulatta dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OMb45709
Clone ID Related Accession (Same CDS sequence)	XM_015112634.1
Accession Version	XM_015112634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CAGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAT GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCACGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCACG 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CTCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG 
CACGTGCTTC AGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GCCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTCTA TTATCTGGAA TACAAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACCG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCTTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCA GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTTATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968120.1
CDS	186..6452
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112634.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112634.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CAGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAT GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCACGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCACG 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CTCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG 
CACGTGCTTC AGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GCCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTCTA TTATCTGGAA TACAAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACCG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACAAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCTTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCA GGGATGAGCC CAACATGAAC 
CCTAAGCTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTTATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112635.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968121.1 Macaca mulatta dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OMb45710
Clone ID Related Accession (Same CDS sequence)	XM_015112635.1
Accession Version	XM_015112635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCAG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGATGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC ACGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACT CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968121.1
CDS	298..6561
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112635.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112635.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCAG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGATGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC ACGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACT CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112636.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968122.1 Macaca mulatta dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OMb45711
Clone ID Related Accession (Same CDS sequence)	XM_015112636.1
Accession Version	XM_015112636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCAGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ATGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCACGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACTCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968122.1
CDS	185..6430
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112636.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112636.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCAGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ATGCCGGTCA CCTGAGCTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG CAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCACGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA ACCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACTCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCACGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACAAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAGCTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112637.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968123.1 Macaca mulatta dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OMb45712
Clone ID Related Accession (Same CDS sequence)	XM_015112637.1
Accession Version	XM_015112637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC AGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGATG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCACGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CTCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACTCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCGCAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCGTGGA GGAGCGTCTG GCTCTGCACG TGCTTCAGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGCCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTCTATTAT CTGGAATACA 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACCGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCT TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCAGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTT ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968123.1
CDS	298..6540
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112637.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112637.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC AGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGATG CCGGTCACCT GAGCTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGCAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCACGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAACC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CTCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCACGCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACTCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCGCAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCGTGGA GGAGCGTCTG GCTCTGCACG TGCTTCAGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGCCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTCTATTAT CTGGAATACA 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACCGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACAAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCT TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCAGGGA TGAGCCCAAC ATGAACCCTA AGCTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTT ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112638.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968124.1 Macaca mulatta dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OMb45713
Clone ID Related Accession (Same CDS sequence)	XM_015112638.1
Accession Version	XM_015112638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CAGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAT GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCACGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968124.1
CDS	185..6388
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112638.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112638.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTT CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACGTAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CAGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAT GCCGGTCACC TGAGCTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGCA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCACGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAAC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACTCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCACGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACAAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAGCTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_015112639.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_014968125.1 Macaca mulatta dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OMb45714
Clone ID Related Accession (Same CDS sequence)	XM_015112639.1
Accession Version	XM_015112639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-20
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014805414.1) and transcript sequence (JV043831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Macaca mulatta Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 147 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCAG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGATGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC ACGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACT CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CACGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CAAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014968125.1
CDS	298..6498
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDVEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLATQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPAQVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_015112639.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015112639.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCGGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTTCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACGTAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCAG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGATGCC GGTCACCTGA GCTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGCAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC ACGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAACCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACT CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CACGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CAAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAG 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832388.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688221.1 Macaca mulatta dysferlin (DYSF), transcript variant X1, mRNA.

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CloneID	OMb68132
Clone ID Related Accession (Same CDS sequence)	XM_028832388.1
Accession Version	XM_028832388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTCCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAACTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688221.1
CDS	106..6549
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832388.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832388.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTCCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAACTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832389.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688222.1 Macaca mulatta dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OMb68133
Clone ID Related Accession (Same CDS sequence)	XM_028832389.1
Accession Version	XM_028832389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGAGGAGAC TGCAGGTGCC GTCAAGCCTT CGAAAGTCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT TCTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAAGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAACTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA GCCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGCTGT GTGCTCCTGC CTTCTTCCTC 
CAGGGCCTTC TCCAAACGTC TGAGGACACA GACCTGCCCT ACCCACCACC CCAGAGGGAG 
GCCAACATCT ACATGGTTCC TCAGAACATC AAGCCAGCGC TCCAGCGTAC CGCCATCGAG 
GTCCTGGCAT GGGGCCTGCG GAACATGAAG AGTTACCAGC TGGCCAGCAT CTCCTCCCCG 
AGCCTTGTGA TAGAGTGTGG GGGCCAGACG GTGCAGTCCT GTGTCATCAG GAACCTCCGG 
AAGAACCCCA ACTTTGACAT CTGTACCCTC TTCATGGAAG TGATGCTGCC CAGGGAGGAG 
CTCTACTGCC CCCCCATCAC CATCAAGGTC ATCGATAACC GCCAGTTTGG CCGCCGGCCT 
GTGGTGGGCC AGTGTACCAT CCGCTCCCTG GAGAGCTTCC TGTGTGACCC CTACTCGGCG 
GAGAGTCCAT CCCCACAGGG TGGCCCAGAC GATGTGAGCC TACTCAGTCC TGGGGAAGAC 
GTGCTCATCG ACATTGATGA CAAGGAGCCC CTCATCCCCA TCCAGCTTGC AGACGGTCTG 
TCGAGCTTGG CCCCTACTAA CACGGCTTCT CCTCCATCCA GTCCTCATGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAA 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688222.1
CDS	376..6816
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832389.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832389.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGAGGAGAC TGCAGGTGCC GTCAAGCCTT CGAAAGTCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT TCTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAAGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAACTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA GCCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGCTGT GTGCTCCTGC CTTCTTCCTC 
CAGGGCCTTC TCCAAACGTC TGAGGACACA GACCTGCCCT ACCCACCACC CCAGAGGGAG 
GCCAACATCT ACATGGTTCC TCAGAACATC AAGCCAGCGC TCCAGCGTAC CGCCATCGAG 
GTCCTGGCAT GGGGCCTGCG GAACATGAAG AGTTACCAGC TGGCCAGCAT CTCCTCCCCG 
AGCCTTGTGA TAGAGTGTGG GGGCCAGACG GTGCAGTCCT GTGTCATCAG GAACCTCCGG 
AAGAACCCCA ACTTTGACAT CTGTACCCTC TTCATGGAAG TGATGCTGCC CAGGGAGGAG 
CTCTACTGCC CCCCCATCAC CATCAAGGTC ATCGATAACC GCCAGTTTGG CCGCCGGCCT 
GTGGTGGGCC AGTGTACCAT CCGCTCCCTG GAGAGCTTCC TGTGTGACCC CTACTCGGCG 
GAGAGTCCAT CCCCACAGGG TGGCCCAGAC GATGTGAGCC TACTCAGTCC TGGGGAAGAC 
GTGCTCATCG ACATTGATGA CAAGGAGCCC CTCATCCCCA TCCAGCTTGC AGACGGTCTG 
TCGAGCTTGG CCCCTACTAA CACGGCTTCT CCTCCATCCA GTCCTCATGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAA 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832390.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688223.1 Macaca mulatta dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OMb68134
Clone ID Related Accession (Same CDS sequence)	XM_028832390.1
Accession Version	XM_028832390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCGCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACTCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CGCAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCGTGGAG 
GAGCGTCTGG CTCTGCACGT GCTTCAGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGCCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTCTATTATC TGGAATACAA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACCGTTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGATA 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCTT GTGTAGCAGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCAGGGAT 
GAGCCCAACA TGAACCCTAA ACTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTCACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTTA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688223.1
CDS	106..6507
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832390.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832390.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCGCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACTCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CGCAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCGTGGAG 
GAGCGTCTGG CTCTGCACGT GCTTCAGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGCCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTCTATTATC TGGAATACAA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACCGTTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGATA 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCTT GTGTAGCAGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCAGGGAT 
GAGCCCAACA TGAACCCTAA ACTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTCACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTTA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832391.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688224.1 Macaca mulatta dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OMb68135
Clone ID Related Accession (Same CDS sequence)	XM_028832391.1
Accession Version	XM_028832391.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAACTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GGAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AGCCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGCTG 
TGTGCTCCTG CCTTCTTCCT CCAGGGCCTT CTCCAAACGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCGCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACTCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CGCAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCGTGGAG 
GAGCGTCTGG CTCTGCACGT GCTTCAGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGCCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTCTATTATC TGGAATACAA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACCGTTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGATA 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCTT GTGTAGCAGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCAGGGAT 
GAGCCCAACA TGAACCCTAA ACTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTCACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTTA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688224.1
CDS	106..6507
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832391.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832391.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAACTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GGAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AGCCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGCTG 
TGTGCTCCTG CCTTCTTCCT CCAGGGCCTT CTCCAAACGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGCTTG CAGACGGTCT GTCGAGCTTG GCCCCTACTA ACACGGCTTC TCCTCCATCC 
AGTCCTCATG AGGAAGAGTT TATCGATTGG TGGAGCAAAT TCTTTGCCTC CATAGGGGAG 
AGGGAAAAGT GCGGCTCCTA CTTGGAGAAG GATTTTGACA CCCTGAAGGT CTATGACACA 
CAGCTGGAGA ATGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTG 
TACCGGGGCA AGACGCAGGA GGAGACAGAA GATCCATCTG TCATTGGCGA ATTTAAGGGC 
CTCTTCAAAA TTTATCCCCT CCCAGAAGAC CCAGCCATCC CCATGCCCCC AAGACAGTTC 
CACCAGCTGG CCGCGCAGGG ACCCCAGGAG TGCTTGGTCC GTATCTACAT TGTCCGAGCA 
TTTGGCCTGC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATCCCCT GCACGTTGGA GCCTGTGTTT 
GGAAAGATGT TTGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GGTCACTCTC 
TACGACTATG ACCTCCTCTC CAAGGATGAA AAGATTGGTG AGACGGTCAT CGACCTGGAG 
AACAGGCTGC TGTCCAAGTT TGCGGCTCGC TGTGGACTCC CGCAGACCTA CTGTGTCTCT 
GGACCGAACC AGTGGCGGGA CCAGCTCCGC CCCTCCCAGC TCCTCCACCT CTTCTGCCAG 
CAGCGCAGAG TCAAGGCGCC TGTGTACCGG ACAGACCGTG TGATGTTTCA GGATAAAGAA 
TACTCCATTG AAGAAATAGA GGCTGGCAGG ATCCCAAACC CACACCTGGG CCCCGTGGAG 
GAGCGTCTGG CTCTGCACGT GCTTCAGCAG CAGGGCCTGG TCCCAGAGCA CGTGGAGTCA 
CGGCCCCTCT ACAGCCCCCT GCAGCCAGAC ATTGAGCAGG GGAAGCTGCA GATGTGGGTC 
GACCTGTTTC CGAAGGCCCT GGGGCGCCCT GGACCTCCCT TCAACATCAC CCCACGGAGA 
GCCAGAAGGT TTTTCCTGCG TTCTATTATC TGGAATACAA GAGATGTGAT CCTGGATGAC 
CTGAGCCTCA CGGGGGAGAA GATGAGCGAC ATTTATGTGA AAGGTTGGAT GATTGGCTTT 
GAAGAACACA AGCAAAAGAC AGACGTGCAT TATCGCTCCC TGGGAGGTGA AGGCAACTTC 
AACTGGAGGT TCATTTTCCC CTTCGACTAC CTGCCGGCTG AGCAAGTCTG TACCGTTGCC 
AAGAAGGATG CCTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGATA 
TTCCAGATCT GGGACAATGA CAAGTTCTCC TTTGATGATT TTCTGGGCTC CCTGCAGCTC 
GATCTCAACC GCATGCCCAA GCCAGCCAAG ACAGCCGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT TCCACCCAGA ATGGTTTGTG TCCCTTTTTG AGCAGAAAAC AGTGAAGGGC 
TGGTGGCCTT GTGTAGCAGA AGAGGGTGAG AAGAAGATAC TGGCGGGCAA GCTGGAAATG 
ACCTTGGAGA TTGTAACAGA GAGTGAGCAT GAGGAGCGGC CCGCTGGCCA GGGCAGGGAT 
GAGCCCAACA TGAACCCTAA ACTTGAGGAC CCAAGGCGCC CCGACACCTC CTTCCTGTGG 
TTCACCTCCC CATACAAGAC CATGAAGTTC ATCCTGTGGC GGCGTTTCCG GTGGGCCATC 
ATCCTCTTTA TCATCCTCTT CATCCTGCTG CTGTTCCTGG CCATCTTCAT CTACGCCTTC 
CCGAACTATG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832392.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688225.1 Macaca mulatta dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OMb68136
Clone ID Related Accession (Same CDS sequence)	XM_028832392.1
Accession Version	XM_028832392.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTCCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAA 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688225.1
CDS	106..6486
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832392.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832392.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGC TGTGTGCTCC TGCCTTCTTC 
CTCCAGGGCC TTCTCCAAAC GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAA 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832393.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688226.1 Macaca mulatta dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OMb68137
Clone ID Related Accession (Same CDS sequence)	XM_028832393.1
Accession Version	XM_028832393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAACTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GGAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AGCCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT CTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCTTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCAGGGATGA GCCCAACATG AACCCTAAAC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTTATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688226.1
CDS	140..6499
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832393.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832393.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAACTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GGAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AGCCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT CTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCTTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCAGGGATGA GCCCAACATG AACCCTAAAC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTTATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832394.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688227.1 Macaca mulatta dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OMb68138
Clone ID Related Accession (Same CDS sequence)	XM_028832394.1
Accession Version	XM_028832394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAACTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGGA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAGC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAACTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688227.1
CDS	376..6732
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832394.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832394.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAACTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGGA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAGC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GCTTGCAGAC GGTCTGTCGA GCTTGGCCCC TACTAACACG 
GCTTCTCCTC CATCCAGTCC TCATGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAACTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832395.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688228.1 Macaca mulatta dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OMb68139
Clone ID Related Accession (Same CDS sequence)	XM_028832395.1
Accession Version	XM_028832395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAACTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA GCCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGCTGT GTGCTCCTGC CTTCTTCCTC CAGGGCCTTC TCCAAACGTC TGAGGACACA 
GACCTGCCCT ACCCACCACC CCAGAGGGAG GCCAACATCT ACATGGTTCC TCAGAACATC 
AAGCCAGCGC TCCAGCGTAC CGCCATCGAG GTCCTGGCAT GGGGCCTGCG GAACATGAAG 
AGTTACCAGC TGGCCAGCAT CTCCTCCCCG AGCCTTGTGA TAGAGTGTGG GGGCCAGACG 
GTGCAGTCCT GTGTCATCAG GAACCTCCGG AAGAACCCCA ACTTTGACAT CTGTACCCTC 
TTCATGGAAG TGATGCTGCC CAGGGAGGAG CTCTACTGCC CCCCCATCAC CATCAAGGTC 
ATCGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGCTCCCTG 
GAGAGCTTCC TGTGTGACCC CTACTCGGCG GAGAGTCCAT CCCCACAGGG TGGCCCAGAC 
GATGTGAGCC TACTCAGTCC TGGGGAAGAC GTGCTCATCG ACATTGATGA CAAGGAGCCC 
CTCATCCCCA TCCAGCTTGC AGACGGTCTG TCGAGCTTGG CCCCTACTAA CACGGCTTCT 
CCTCCATCCA GTCCTCATGA GGAAGAGTTT ATCGATTGGT GGAGCAAATT CTTTGCCTCC 
ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC TTGGAGAAGG ATTTTGACAC CCTGAAGGTC 
TATGACACAC AGCTGGAGAA TGTGGAGGCC TTTGAGGGCC TGTCTGACTT TTGTAACACC 
TTCAAGCTGT ACCGGGGCAA GACGCAGGAG GAGACAGAAG ATCCATCTGT CATTGGCGAA 
TTTAAGGGCC TCTTCAAAAT TTATCCCCTC CCAGAAGACC CAGCCATCCC CATGCCCCCA 
AGACAGTTCC ACCAGCTGGC CGCGCAGGGA CCCCAGGAGT GCTTGGTCCG TATCTACATT 
GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC CCCAATGGGA AGTGTGATCC TTACATCAAG 
ATCTCCATAG GGAAGAAATC AGTGAGTGAC CAGGATAACT ACATCCCCTG CACGTTGGAG 
CCTGTGTTTG GAAAGATGTT TGAGCTGACC TGCACTCTGC CTCTGGAGAA GGACCTGAAG 
GTCACTCTCT ACGACTATGA CCTCCTCTCC AAGGATGAAA AGATTGGTGA GACGGTCATC 
GACCTGGAGA ACAGGCTGCT GTCCAAGTTT GCGGCTCGCT GTGGACTCCC GCAGACCTAC 
TGTGTCTCTG GACCGAACCA GTGGCGGGAC CAGCTCCGCC CCTCCCAGCT CCTCCACCTC 
TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT GTGTACCGGA CAGACCGTGT GATGTTTCAG 
GATAAAGAAT ACTCCATTGA AGAAATAGAG GCTGGCAGGA TCCCAAACCC ACACCTGGGC 
CCCGTGGAGG AGCGTCTGGC TCTGCACGTG CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC 
GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG CAGCCAGACA TTGAGCAGGG GAAGCTGCAG 
ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG GGGCGCCCTG GACCTCCCTT CAACATCACC 
CCACGGAGAG CCAGAAGGTT TTTCCTGCGT TCTATTATCT GGAATACAAG AGATGTGATC 
CTGGATGACC TGAGCCTCAC GGGGGAGAAG ATGAGCGACA TTTATGTGAA AGGTTGGATG 
ATTGGCTTTG AAGAACACAA GCAAAAGACA GACGTGCATT ATCGCTCCCT GGGAGGTGAA 
GGCAACTTCA ACTGGAGGTT CATTTTCCCC TTCGACTACC TGCCGGCTGA GCAAGTCTGT 
ACCGTTGCCA AGAAGGATGC CTTCTGGAGG CTGGACAAGA CTGAGAGCAA AATCCCAGCA 
CGAGTGATAT TCCAGATCTG GGACAATGAC AAGTTCTCCT TTGATGATTT TCTGGGCTCC 
CTGCAGCTCG ATCTCAACCG CATGCCCAAG CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG 
GACCAGCTGG ATGACACTTT CCACCCAGAA TGGTTTGTGT CCCTTTTTGA GCAGAAAACA 
GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA GAGGGTGAGA AGAAGATACT GGCGGGCAAG 
CTGGAAATGA CCTTGGAGAT TGTAACAGAG AGTGAGCATG AGGAGCGGCC CGCTGGCCAG 
GGCAGGGATG AGCCCAACAT GAACCCTAAA CTTGAGGACC CAAGGCGCCC CGACACCTCC 
TTCCTGTGGT TCACCTCCCC ATACAAGACC ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG 
TGGGCCATCA TCCTCTTTAT CATCCTCTTC ATCCTGCTGC TGTTCCTGGC CATCTTCATC 
TACGCCTTCC CGAACTATGC TGCCATGAAG CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688228.1
CDS	106..6456
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832395.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832395.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAACTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA GCCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGCTGT GTGCTCCTGC CTTCTTCCTC CAGGGCCTTC TCCAAACGTC TGAGGACACA 
GACCTGCCCT ACCCACCACC CCAGAGGGAG GCCAACATCT ACATGGTTCC TCAGAACATC 
AAGCCAGCGC TCCAGCGTAC CGCCATCGAG GTCCTGGCAT GGGGCCTGCG GAACATGAAG 
AGTTACCAGC TGGCCAGCAT CTCCTCCCCG AGCCTTGTGA TAGAGTGTGG GGGCCAGACG 
GTGCAGTCCT GTGTCATCAG GAACCTCCGG AAGAACCCCA ACTTTGACAT CTGTACCCTC 
TTCATGGAAG TGATGCTGCC CAGGGAGGAG CTCTACTGCC CCCCCATCAC CATCAAGGTC 
ATCGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGCTCCCTG 
GAGAGCTTCC TGTGTGACCC CTACTCGGCG GAGAGTCCAT CCCCACAGGG TGGCCCAGAC 
GATGTGAGCC TACTCAGTCC TGGGGAAGAC GTGCTCATCG ACATTGATGA CAAGGAGCCC 
CTCATCCCCA TCCAGCTTGC AGACGGTCTG TCGAGCTTGG CCCCTACTAA CACGGCTTCT 
CCTCCATCCA GTCCTCATGA GGAAGAGTTT ATCGATTGGT GGAGCAAATT CTTTGCCTCC 
ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC TTGGAGAAGG ATTTTGACAC CCTGAAGGTC 
TATGACACAC AGCTGGAGAA TGTGGAGGCC TTTGAGGGCC TGTCTGACTT TTGTAACACC 
TTCAAGCTGT ACCGGGGCAA GACGCAGGAG GAGACAGAAG ATCCATCTGT CATTGGCGAA 
TTTAAGGGCC TCTTCAAAAT TTATCCCCTC CCAGAAGACC CAGCCATCCC CATGCCCCCA 
AGACAGTTCC ACCAGCTGGC CGCGCAGGGA CCCCAGGAGT GCTTGGTCCG TATCTACATT 
GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC CCCAATGGGA AGTGTGATCC TTACATCAAG 
ATCTCCATAG GGAAGAAATC AGTGAGTGAC CAGGATAACT ACATCCCCTG CACGTTGGAG 
CCTGTGTTTG GAAAGATGTT TGAGCTGACC TGCACTCTGC CTCTGGAGAA GGACCTGAAG 
GTCACTCTCT ACGACTATGA CCTCCTCTCC AAGGATGAAA AGATTGGTGA GACGGTCATC 
GACCTGGAGA ACAGGCTGCT GTCCAAGTTT GCGGCTCGCT GTGGACTCCC GCAGACCTAC 
TGTGTCTCTG GACCGAACCA GTGGCGGGAC CAGCTCCGCC CCTCCCAGCT CCTCCACCTC 
TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT GTGTACCGGA CAGACCGTGT GATGTTTCAG 
GATAAAGAAT ACTCCATTGA AGAAATAGAG GCTGGCAGGA TCCCAAACCC ACACCTGGGC 
CCCGTGGAGG AGCGTCTGGC TCTGCACGTG CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC 
GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG CAGCCAGACA TTGAGCAGGG GAAGCTGCAG 
ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG GGGCGCCCTG GACCTCCCTT CAACATCACC 
CCACGGAGAG CCAGAAGGTT TTTCCTGCGT TCTATTATCT GGAATACAAG AGATGTGATC 
CTGGATGACC TGAGCCTCAC GGGGGAGAAG ATGAGCGACA TTTATGTGAA AGGTTGGATG 
ATTGGCTTTG AAGAACACAA GCAAAAGACA GACGTGCATT ATCGCTCCCT GGGAGGTGAA 
GGCAACTTCA ACTGGAGGTT CATTTTCCCC TTCGACTACC TGCCGGCTGA GCAAGTCTGT 
ACCGTTGCCA AGAAGGATGC CTTCTGGAGG CTGGACAAGA CTGAGAGCAA AATCCCAGCA 
CGAGTGATAT TCCAGATCTG GGACAATGAC AAGTTCTCCT TTGATGATTT TCTGGGCTCC 
CTGCAGCTCG ATCTCAACCG CATGCCCAAG CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG 
GACCAGCTGG ATGACACTTT CCACCCAGAA TGGTTTGTGT CCCTTTTTGA GCAGAAAACA 
GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA GAGGGTGAGA AGAAGATACT GGCGGGCAAG 
CTGGAAATGA CCTTGGAGAT TGTAACAGAG AGTGAGCATG AGGAGCGGCC CGCTGGCCAG 
GGCAGGGATG AGCCCAACAT GAACCCTAAA CTTGAGGACC CAAGGCGCCC CGACACCTCC 
TTCCTGTGGT TCACCTCCCC ATACAAGACC ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG 
TGGGCCATCA TCCTCTTTAT CATCCTCTTC ATCCTGCTGC TGTTCCTGGC CATCTTCATC 
TACGCCTTCC CGAACTATGC TGCCATGAAG CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832396.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688229.1 Macaca mulatta dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OMb68140
Clone ID Related Accession (Same CDS sequence)	XM_028832396.1
Accession Version	XM_028832396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAACTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGGAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAGCC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGCTGTGTG CTCCTGCCTT CTTCCTCCAG GGCCTTCTCC AAACGTCTGA GGACACAGAC 
CTGCCCTACC CACCACCCCA GAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATCGAGGTC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAGCATCTC CTCCCCGAGC CTTGTGATAG AGTGTGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TTGACATCTG TACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCAT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCATCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CTACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATGAGGA AGAGTTTATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGCGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCGC GCAGGGACCC CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GTTGGAGCCT 
GTGTTTGGAA AGATGTTTGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACTCTCTACG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGTGAGAC GGTCATCGAC 
CTGGAGAACA GGCTGCTGTC CAAGTTTGCG GCTCGCTGTG GACTCCCGCA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC GCAGAGTCAA GGCGCCTGTG TACCGGACAG ACCGTGTGAT GTTTCAGGAT 
AAAGAATACT CCATTGAAGA AATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCC 
GTGGAGGAGC GTCTGGCTCT GCACGTGCTT CAGCAGCAGG GCCTGGTCCC AGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGCCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTCT ATTATCTGGA ATACAAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GCTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCAT TTTCCCCTTC GACTACCTGC CGGCTGAGCA AGTCTGTACC 
GTTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGATATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCTTGTGT AGCAGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC 
AGGGATGAGC CCAACATGAA CCCTAAACTT GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTCA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTTATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688229.1
CDS	376..6723
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832396.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832396.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAACTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGGAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAGCC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGCTGTGTG CTCCTGCCTT CTTCCTCCAG GGCCTTCTCC AAACGTCTGA GGACACAGAC 
CTGCCCTACC CACCACCCCA GAGGGAGGCC AACATCTACA TGGTTCCTCA GAACATCAAG 
CCAGCGCTCC AGCGTACCGC CATCGAGGTC CTGGCATGGG GCCTGCGGAA CATGAAGAGT 
TACCAGCTGG CCAGCATCTC CTCCCCGAGC CTTGTGATAG AGTGTGGGGG CCAGACGGTG 
CAGTCCTGTG TCATCAGGAA CCTCCGGAAG AACCCCAACT TTGACATCTG TACCCTCTTC 
ATGGAAGTGA TGCTGCCCAG GGAGGAGCTC TACTGCCCCC CCATCACCAT CAAGGTCATC 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG CTCCCTGGAG 
AGCTTCCTGT GTGACCCCTA CTCGGCGGAG AGTCCATCCC CACAGGGTGG CCCAGACGAT 
GTGAGCCTAC TCAGTCCTGG GGAAGACGTG CTCATCGACA TTGATGACAA GGAGCCCCTC 
ATCCCCATCC AGCTTGCAGA CGGTCTGTCG AGCTTGGCCC CTACTAACAC GGCTTCTCCT 
CCATCCAGTC CTCATGAGGA AGAGTTTATC GATTGGTGGA GCAAATTCTT TGCCTCCATA 
GGGGAGAGGG AAAAGTGCGG CTCCTACTTG GAGAAGGATT TTGACACCCT GAAGGTCTAT 
GACACACAGC TGGAGAATGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTGTACC GGGGCAAGAC GCAGGAGGAG ACAGAAGATC CATCTGTCAT TGGCGAATTT 
AAGGGCCTCT TCAAAATTTA TCCCCTCCCA GAAGACCCAG CCATCCCCAT GCCCCCAAGA 
CAGTTCCACC AGCTGGCCGC GCAGGGACCC CAGGAGTGCT TGGTCCGTAT CTACATTGTC 
CGAGCATTTG GCCTGCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TCCCCTGCAC GTTGGAGCCT 
GTGTTTGGAA AGATGTTTGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGGTC 
ACTCTCTACG ACTATGACCT CCTCTCCAAG GATGAAAAGA TTGGTGAGAC GGTCATCGAC 
CTGGAGAACA GGCTGCTGTC CAAGTTTGCG GCTCGCTGTG GACTCCCGCA GACCTACTGT 
GTCTCTGGAC CGAACCAGTG GCGGGACCAG CTCCGCCCCT CCCAGCTCCT CCACCTCTTC 
TGCCAGCAGC GCAGAGTCAA GGCGCCTGTG TACCGGACAG ACCGTGTGAT GTTTCAGGAT 
AAAGAATACT CCATTGAAGA AATAGAGGCT GGCAGGATCC CAAACCCACA CCTGGGCCCC 
GTGGAGGAGC GTCTGGCTCT GCACGTGCTT CAGCAGCAGG GCCTGGTCCC AGAGCACGTG 
GAGTCACGGC CCCTCTACAG CCCCCTGCAG CCAGACATTG AGCAGGGGAA GCTGCAGATG 
TGGGTCGACC TGTTTCCGAA GGCCCTGGGG CGCCCTGGAC CTCCCTTCAA CATCACCCCA 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTCT ATTATCTGGA ATACAAGAGA TGTGATCCTG 
GATGACCTGA GCCTCACGGG GGAGAAGATG AGCGACATTT ATGTGAAAGG TTGGATGATT 
GGCTTTGAAG AACACAAGCA AAAGACAGAC GTGCATTATC GCTCCCTGGG AGGTGAAGGC 
AACTTCAACT GGAGGTTCAT TTTCCCCTTC GACTACCTGC CGGCTGAGCA AGTCTGTACC 
GTTGCCAAGA AGGATGCCTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGATATTCC AGATCTGGGA CAATGACAAG TTCTCCTTTG ATGATTTTCT GGGCTCCCTG 
CAGCTCGATC TCAACCGCAT GCCCAAGCCA GCCAAGACAG CCGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTTCCA CCCAGAATGG TTTGTGTCCC TTTTTGAGCA GAAAACAGTG 
AAGGGCTGGT GGCCTTGTGT AGCAGAAGAG GGTGAGAAGA AGATACTGGC GGGCAAGCTG 
GAAATGACCT TGGAGATTGT AACAGAGAGT GAGCATGAGG AGCGGCCCGC TGGCCAGGGC 
AGGGATGAGC CCAACATGAA CCCTAAACTT GAGGACCCAA GGCGCCCCGA CACCTCCTTC 
CTGTGGTTCA CCTCCCCATA CAAGACCATG AAGTTCATCC TGTGGCGGCG TTTCCGGTGG 
GCCATCATCC TCTTTATCAT CCTCTTCATC CTGCTGCTGT TCCTGGCCAT CTTCATCTAC 
GCCTTCCCGA ACTATGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832397.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688230.1 Macaca mulatta dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OMb68141
Clone ID Related Accession (Same CDS sequence)	XM_028832397.1
Accession Version	XM_028832397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CGCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGGT CACTCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCGC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CGTGGAGGAG 
CGTCTGGCTC TGCACGTGCT TCAGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTC TATTATCTGG AATACAAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CAGGGATGAG 
CCCAACATGA ACCCTAAACT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTTATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688230.1
CDS	136..6474
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832397.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832397.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGAGGA GACTGCAGGT GCCGTCAAGC CTTCGAAAGT CTCAGACCTG 
GATGACCACC TGGGCTTCCT CCCCACTTTT GGGCCCTGCT ACATCAACCT CTATGGCAGT 
CCCAGAGAGT TCACGGGCTT CCTGGACCCC TACGCAGAGC TCAACACAGG CAAGGGGGAA 
GGTGTGGCTT ATCGTGGCCG GCTTCTGCTT TCCCTGGAGA CCAAGCTGGT GGAGCACAGT 
GAGCAGAAGG TGGAGGACCT TCCTGCGGAT GACATCCTCC GGGTGGAGAA GTACCTTAGG 
AGGCGCAAGT ACTCCCTGTT TGCGGCCTTC TACTCGGCCA CCATGCTGCA GGATGTGGAT 
GACGCCATCC AGTTTGAGGT CAGCATCGGG AACTATGGGA ACAAGTTCGA CATGACCTGC 
CTGCCGCTGG CCTCCACCAC TCAGTATAGC CGTGCAGTCT TTGACGGGTG CCACTACTAC 
TACCTACCCT GGGGCAACGT GAAACCTGTG GTGGTGCTGT CATCCTACTG GGAGGACATC 
AGCCATAGAA TCGAGACTCA GAACCAGCTG CTTGGGATTG CTGACCGGCT GGAAGCTGGC 
CTGGAGCAGG TCCACCTGGC CCTGAAGGCG CAGTGCTCCA CGGAGGACGT GGACTCACTG 
GTGGCTCAGA TGATGGATGA GCTCATCGCA GGCTGCAGCC AGCCTCTGGG TGATATCCAT 
GAGATACCCT CTGCCACCCA CCTGGACGAG TACCTGTACC GGCTGCGCAC CCGTCACCTG 
AGCCAAATCA CGGAGGCTGC CCTGGCCCTG AAGCTTGGCC ACAGTGAGCT CCCTGCTGCT 
CTGGATCAGG CTGAGGACTG GCTCCTGCGT CTGCGTGCCC TCGCTGAGGA GCCCCAGAAC 
AGCCTGCCGG ATATCATCAT CTGGATGCTG CAGGGAGACA AGCGTGTGGC ATACCAGCGG 
GTGCCCGCCC ACCAAGTCCT CTTCTCCCGG CGGGGTGCCA ACTTCTGTGG CAAGAATTGT 
GGGAAGTTGC AGACAATCTT TCTGAAATAT CCGATGGAGA AGGTGCCTGG GGCCCGGATG 
CCAGTGCAGA TACGGGTCAA GCTGTGGTTT GGGCTCTCTG TGGATGAGAA GGAGTTCAAC 
CAGTTTGCTG AGGGGAAGCT GTCTGTCTTT GCTGAAACCT ATGAGAACCA GACCAAGTTG 
GCCCTGGTCG GGAACTGGGG CACAACGGGC CTCACCTACC CCAAGTTTTC TGACGTCACC 
GGCAAGATCA AGCTACCCAA AGAGAGCTTC CGCCCCTCGG CCGGCTGGAC CTGGGCTGGA 
GATTGGTTCG TGTGTCCGGA GAAGACCCTG CTCCATGACA TGGACGCCGG TCACCTGAAC 
TTTGTGGAGG AGGTGTTTGA GAACCAGACC CGGCTTCCCG GAGGCCAGTG GATCTACATG 
AGTGACAACT ACACCGATGT GAACGGGGAG AAGGTGCTTC CCAAAGATGA CATTGAGTGC 
CCACTGGGCT GGAAGTGGGA AGATGAGGAA TGGTCCACAG ACCTCAACCG GGCTGTCGAT 
GAGCAAGGCT GGGAGTATAG CATCACCATC CCCCCGGACC GGAAGCCGAA GCACTGGGTC 
CCTGCTGAGA AGATGTACTA CACACACCGA CGGCGGCGCT GGGTGCGCCT GCGGAGGAGG 
GATCTCAGCC AGATGGAAGC GCTGAAGAGG CATAGGCAGG CGGAGGCAGA GGGCGAGGGC 
TGGGAGTACG CCTCTCTTTT TGGCTGGAAG TTCCACCTCG AGTACCGCAA GACAGACGCC 
TTCCGCCGCC GCCGCTGGCG CCGCCGCATG GAGCCCCTGG AGAAGACGGG GCCTGCAGCT 
GTGTTTGCCC TCGAGGGGGC CCTGGGCGGC GTGGTGGACG ACAAGAGTGA AGATTCCATG 
TCCGTCTCCA CCTTGAGCTT TGGTGTGAAC AGACCCACAA TTTCCTGCAT ATTTGACTAT 
GGGAACCGCT ACCATCTACG CTGCTACATG TACCAGGCCC GGGACCTGGC TGCGATGGAC 
AAGGACTCTT TTTCTGATCC CTATGCCATC GTCTCCTTCC TGCACCAGAG CCAGAAGACG 
GTGGTGGTGA AGAACACCCT TAACCCCACC TGGGACCAGA CGCTCATCTT CTACGAGATC 
GAGATCTTCG GCGAGCCGGC CACAGTTGCT GAGCAGCCGC CCAGCATTGT GGTGGAGCTG 
TACGACCATG ACACTTACGG CGCAGACGAG TTTATGGGCC GCTGCATCTG TCAGCCGAGT 
TTGGAACGGA CGCCCCGACT GGCCTGGTTC CCACTGACCA GGGGCAGCCA GCCGACGGGG 
GAGCTGCTGG CCTCTTTTGA GCTCATCCAG AGAGAGAAGC CGGCCATCCA CCATATTCCT 
GGTTTTGAGG TGCAGGAGAC ATCGAGGATC CTGGATGAGT CTGAGGACAC AGACCTGCCC 
TACCCACCAC CCCAGAGGGA GGCCAACATC TACATGGTTC CTCAGAACAT CAAGCCAGCG 
CTCCAGCGTA CCGCCATCGA GGTCCTGGCA TGGGGCCTGC GGAACATGAA GAGTTACCAG 
CTGGCCAGCA TCTCCTCCCC GAGCCTTGTG ATAGAGTGTG GGGGCCAGAC GGTGCAGTCC 
TGTGTCATCA GGAACCTCCG GAAGAACCCC AACTTTGACA TCTGTACCCT CTTCATGGAA 
GTGATGCTGC CCAGGGAGGA GCTCTACTGC CCCCCCATCA CCATCAAGGT CATCGATAAC 
CGCCAGTTTG GCCGCCGGCC TGTGGTGGGC CAGTGTACCA TCCGCTCCCT GGAGAGCTTC 
CTGTGTGACC CCTACTCGGC GGAGAGTCCA TCCCCACAGG GTGGCCCAGA CGATGTGAGC 
CTACTCAGTC CTGGGGAAGA CGTGCTCATC GACATTGATG ACAAGGAGCC CCTCATCCCC 
ATCCAGGAGG AAGAGTTTAT CGATTGGTGG AGCAAATTCT TTGCCTCCAT AGGGGAGAGG 
GAAAAGTGCG GCTCCTACTT GGAGAAGGAT TTTGACACCC TGAAGGTCTA TGACACACAG 
CTGGAGAATG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTGTAC 
CGGGGCAAGA CGCAGGAGGA GACAGAAGAT CCATCTGTCA TTGGCGAATT TAAGGGCCTC 
TTCAAAATTT ATCCCCTCCC AGAAGACCCA GCCATCCCCA TGCCCCCAAG ACAGTTCCAC 
CAGCTGGCCG CGCAGGGACC CCAGGAGTGC TTGGTCCGTA TCTACATTGT CCGAGCATTT 
GGCCTGCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA 
AAGATGTTTG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGGT CACTCTCTAC 
GACTATGACC TCCTCTCCAA GGATGAAAAG ATTGGTGAGA CGGTCATCGA CCTGGAGAAC 
AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT GGACTCCCGC AGACCTACTG TGTCTCTGGA 
CCGAACCAGT GGCGGGACCA GCTCCGCCCC TCCCAGCTCC TCCACCTCTT CTGCCAGCAG 
CGCAGAGTCA AGGCGCCTGT GTACCGGACA GACCGTGTGA TGTTTCAGGA TAAAGAATAC 
TCCATTGAAG AAATAGAGGC TGGCAGGATC CCAAACCCAC ACCTGGGCCC CGTGGAGGAG 
CGTCTGGCTC TGCACGTGCT TCAGCAGCAG GGCCTGGTCC CAGAGCACGT GGAGTCACGG 
CCCCTCTACA GCCCCCTGCA GCCAGACATT GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC 
CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA CCTCCCTTCA ACATCACCCC ACGGAGAGCC 
AGAAGGTTTT TCCTGCGTTC TATTATCTGG AATACAAGAG ATGTGATCCT GGATGACCTG 
AGCCTCACGG GGGAGAAGAT GAGCGACATT TATGTGAAAG GTTGGATGAT TGGCTTTGAA 
GAACACAAGC AAAAGACAGA CGTGCATTAT CGCTCCCTGG GAGGTGAAGG CAACTTCAAC 
TGGAGGTTCA TTTTCCCCTT CGACTACCTG CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG 
AAGGATGCCT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGATATTC 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GATGATTTTC TGGGCTCCCT GCAGCTCGAT 
CTCAACCGCA TGCCCAAGCC AGCCAAGACA GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCAGAATG GTTTGTGTCC CTTTTTGAGC AGAAAACAGT GAAGGGCTGG 
TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG AAGATACTGG CGGGCAAGCT GGAAATGACC 
TTGGAGATTG TAACAGAGAG TGAGCATGAG GAGCGGCCCG CTGGCCAGGG CAGGGATGAG 
CCCAACATGA ACCCTAAACT TGAGGACCCA AGGCGCCCCG ACACCTCCTT CCTGTGGTTC 
ACCTCCCCAT ACAAGACCAT GAAGTTCATC CTGTGGCGGC GTTTCCGGTG GGCCATCATC 
CTCTTTATCA TCCTCTTCAT CCTGCTGCTG TTCCTGGCCA TCTTCATCTA CGCCTTCCCG 
AACTATGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832398.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688231.1 Macaca mulatta dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OMb68142
Clone ID Related Accession (Same CDS sequence)	XM_028832398.1
Accession Version	XM_028832398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGAGGAGAC TGCAGGTGCC GTCAAGCCTT CGAAAGTCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT TCTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAAGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAACTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA GCCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGATA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCGC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGGTCAC TCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCGCAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCGT GGAGGAGCGT 
CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG CCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTCTAT TATCTGGAAT ACAAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACCGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCAG GGATGAGCCC 
AACATGAACC CTAAACTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTTATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688231.1
CDS	377..6712
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832398.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832398.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGAGGAGAC TGCAGGTGCC GTCAAGCCTT CGAAAGTCTC AGACCTGGAT 
GACCACCTGG GCTTCCTCCC CACTTTTGGG CCCTGCTACA TCAACCTCTA TGGCAGTCCC 
AGAGAGTTCA CGGGCTTCCT GGACCCCTAC GCAGAGCTCA ACACAGGCAA GGGGGAAGGT 
GTGGCTTATC GTGGCCGGCT TCTGCTTTCC CTGGAGACCA AGCTGGTGGA GCACAGTGAG 
CAGAAGGTGG AGGACCTTCC TGCGGATGAC ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG 
CGCAAGTACT CCCTGTTTGC GGCCTTCTAC TCGGCCACCA TGCTGCAGGA TGTGGATGAC 
GCCATCCAGT TTGAGGTCAG CATCGGGAAC TATGGGAACA AGTTCGACAT GACCTGCCTG 
CCGCTGGCCT CCACCACTCA GTATAGCCGT GCAGTCTTTG ACGGGTGCCA CTACTACTAC 
CTACCCTGGG GCAACGTGAA ACCTGTGGTG GTGCTGTCAT CCTACTGGGA GGACATCAGC 
CATAGAATCG AGACTCAGAA CCAGCTGCTT GGGATTGCTG ACCGGCTGGA AGCTGGCCTG 
GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG TGCTCCACGG AGGACGTGGA CTCACTGGTG 
GCTCAGATGA TGGATGAGCT CATCGCAGGC TGCAGCCAGC CTCTGGGTGA TATCCATGAG 
ATACCCTCTG CCACCCACCT GGACGAGTAC CTGTACCGGC TGCGCACCCG TCACCTGAGC 
CAAATCACGG AGGCTGCCCT GGCCCTGAAG CTTGGCCACA GTGAGCTCCC TGCTGCTCTG 
GATCAGGCTG AGGACTGGCT CCTGCGTCTG CGTGCCCTCG CTGAGGAGCC CCAGAACAGC 
CTGCCGGATA TCATCATCTG GATGCTGCAG GGAGACAAGC GTGTGGCATA CCAGCGGGTG 
CCCGCCCACC AAGTCCTCTT CTCCCGGCGG GGTGCCAACT TCTGTGGCAA GAATTGTGGG 
AAGTTGCAGA CAATCTTTCT GAAATATCCG ATGGAGAAGG TGCCTGGGGC CCGGATGCCA 
GTGCAGATAC GGGTCAAGCT GTGGTTTGGG CTCTCTGTGG ATGAGAAGGA GTTCAACCAG 
TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT GAAACCTATG AGAACCAGAC CAAGTTGGCC 
CTGGTCGGGA ACTGGGGCAC AACGGGCCTC ACCTACCCCA AGTTTTCTGA CGTCACCGGC 
AAGATCAAGC TACCCAAAGA GAGCTTCCGC CCCTCGGCCG GCTGGACCTG GGCTGGAGAT 
TGGTTCGTGT GTCCGGAGAA GACCCTGCTC CATGACATGG ACGCCGGTCA CCTGAACTTT 
GTGGAGGAGG TGTTTGAGAA CCAGACCCGG CTTCCCGGAG GCCAGTGGAT CTACATGAGT 
GACAACTACA CCGATGTGAA CGGGGAGAAG GTGCTTCCCA AAGATGACAT TGAGTGCCCA 
CTGGGCTGGA AGTGGGAAGA TGAGGAATGG TCCACAGACC TCAACCGGGC TGTCGATGAG 
CAAGGCTGGG AGTATAGCAT CACCATCCCC CCGGACCGGA AGCCGAAGCA CTGGGTCCCT 
GCTGAGAAGA TGTACTACAC ACACCGACGG CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT 
CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG 
GAGTACGCCT CTCTTTTTGG CTGGAAGTTC CACCTCGAGT ACCGCAAGAC AGACGCCTTC 
CGCCGCCGCC GCTGGCGCCG CCGCATGGAG CCCCTGGAGA AGACGGGGCC TGCAGCTGTG 
TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG GTGGACGACA AGAGTGAAGA TTCCATGTCC 
GTCTCCACCT TGAGCTTTGG TGTGAACAGA CCCACAATTT CCTGCATATT TGACTATGGG 
AACCGCTACC ATCTACGCTG CTACATGTAC CAGGCCCGGG ACCTGGCTGC GATGGACAAG 
GACTCTTTTT CTGATCCCTA TGCCATCGTC TCCTTCCTGC ACCAGAGCCA GAAGACGGTG 
GTGGTGAAGA ACACCCTTAA CCCCACCTGG GACCAGACGC TCATCTTCTA CGAGATCGAG 
ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG CAGCCGCCCA GCATTGTGGT GGAGCTGTAC 
GACCATGACA CTTACGGCGC AGACGAGTTT ATGGGCCGCT GCATCTGTCA GCCGAGTTTG 
GAACGGACGC CCCGACTGGC CTGGTTCCCA CTGACCAGGG GCAGCCAGCC GACGGGGGAG 
CTGCTGGCCT CTTTTGAGCT CATCCAGAGA GAGAAGCCGG CCATCCACCA TATTCCTGGT 
TTTGAGGTGC AGGAGACATC GAGGATCCTG GATGAGTCTG AGGACACAGA CCTGCCCTAC 
CCACCACCCC AGAGGGAGGC CAACATCTAC ATGGTTCCTC AGAACATCAA GCCAGCGCTC 
CAGCGTACCG CCATCGAGGT CCTGGCATGG GGCCTGCGGA ACATGAAGAG TTACCAGCTG 
GCCAGCATCT CCTCCCCGAG CCTTGTGATA GAGTGTGGGG GCCAGACGGT GCAGTCCTGT 
GTCATCAGGA ACCTCCGGAA GAACCCCAAC TTTGACATCT GTACCCTCTT CATGGAAGTG 
ATGCTGCCCA GGGAGGAGCT CTACTGCCCC CCCATCACCA TCAAGGTCAT CGATAACCGC 
CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG TGTACCATCC GCTCCCTGGA GAGCTTCCTG 
TGTGACCCCT ACTCGGCGGA GAGTCCATCC CCACAGGGTG GCCCAGACGA TGTGAGCCTA 
CTCAGTCCTG GGGAAGACGT GCTCATCGAC ATTGATGACA AGGAGCCCCT CATCCCCATC 
CAGGAGGAAG AGTTTATCGA TTGGTGGAGC AAATTCTTTG CCTCCATAGG GGAGAGGGAA 
AAGTGCGGCT CCTACTTGGA GAAGGATTTT GACACCCTGA AGGTCTATGA CACACAGCTG 
GAGAATGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTGTACCGG 
GGCAAGACGC AGGAGGAGAC AGAAGATCCA TCTGTCATTG GCGAATTTAA GGGCCTCTTC 
AAAATTTATC CCCTCCCAGA AGACCCAGCC ATCCCCATGC CCCCAAGACA GTTCCACCAG 
CTGGCCGCGC AGGGACCCCA GGAGTGCTTG GTCCGTATCT ACATTGTCCG AGCATTTGGC 
CTGCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATC CCCTGCACGT TGGAGCCTGT GTTTGGAAAG 
ATGTTTGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGGTCAC TCTCTACGAC 
TATGACCTCC TCTCCAAGGA TGAAAAGATT GGTGAGACGG TCATCGACCT GGAGAACAGG 
CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA CTCCCGCAGA CCTACTGTGT CTCTGGACCG 
AACCAGTGGC GGGACCAGCT CCGCCCCTCC CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC 
AGAGTCAAGG CGCCTGTGTA CCGGACAGAC CGTGTGATGT TTCAGGATAA AGAATACTCC 
ATTGAAGAAA TAGAGGCTGG CAGGATCCCA AACCCACACC TGGGCCCCGT GGAGGAGCGT 
CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC CTGGTCCCAG AGCACGTGGA GTCACGGCCC 
CTCTACAGCC CCCTGCAGCC AGACATTGAG CAGGGGAAGC TGCAGATGTG GGTCGACCTG 
TTTCCGAAGG CCCTGGGGCG CCCTGGACCT CCCTTCAACA TCACCCCACG GAGAGCCAGA 
AGGTTTTTCC TGCGTTCTAT TATCTGGAAT ACAAGAGATG TGATCCTGGA TGACCTGAGC 
CTCACGGGGG AGAAGATGAG CGACATTTAT GTGAAAGGTT GGATGATTGG CTTTGAAGAA 
CACAAGCAAA AGACAGACGT GCATTATCGC TCCCTGGGAG GTGAAGGCAA CTTCAACTGG 
AGGTTCATTT TCCCCTTCGA CTACCTGCCG GCTGAGCAAG TCTGTACCGT TGCCAAGAAG 
GATGCCTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GATATTCCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAT GATTTTCTGG GCTCCCTGCA GCTCGATCTC 
AACCGCATGC CCAAGCCAGC CAAGACAGCC GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CAGAATGGTT TGTGTCCCTT TTTGAGCAGA AAACAGTGAA GGGCTGGTGG 
CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG ATACTGGCGG GCAAGCTGGA AATGACCTTG 
GAGATTGTAA CAGAGAGTGA GCATGAGGAG CGGCCCGCTG GCCAGGGCAG GGATGAGCCC 
AACATGAACC CTAAACTTGA GGACCCAAGG CGCCCCGACA CCTCCTTCCT GTGGTTCACC 
TCCCCATACA AGACCATGAA GTTCATCCTG TGGCGGCGTT TCCGGTGGGC CATCATCCTC 
TTTATCATCC TCTTCATCCT GCTGCTGTTC CTGGCCATCT TCATCTACGC CTTCCCGAAC 
TATGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832399.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688232.1 Macaca mulatta dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OMb68143
Clone ID Related Accession (Same CDS sequence)	XM_028832399.1
Accession Version	XM_028832399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAACTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA GCCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CGCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACTCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCGC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CGTGGAGGAG CGTCTGGCTC TGCACGTGCT TCAGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTC TATTATCTGG 
AATACAAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CAGGGATGAG CCCAACATGA ACCCTAAACT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTTATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688232.1
CDS	136..6444
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832399.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832399.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAACTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA GCCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGCTTGCAG ACGGTCTGTC GAGCTTGGCC 
CCTACTAACA CGGCTTCTCC TCCATCCAGT CCTCATGAGG AAGAGTTTAT CGATTGGTGG 
AGCAAATTCT TTGCCTCCAT AGGGGAGAGG GAAAAGTGCG GCTCCTACTT GGAGAAGGAT 
TTTGACACCC TGAAGGTCTA TGACACACAG CTGGAGAATG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTGTAC CGGGGCAAGA CGCAGGAGGA GACAGAAGAT 
CCATCTGTCA TTGGCGAATT TAAGGGCCTC TTCAAAATTT ATCCCCTCCC AGAAGACCCA 
GCCATCCCCA TGCCCCCAAG ACAGTTCCAC CAGCTGGCCG CGCAGGGACC CCAGGAGTGC 
TTGGTCCGTA TCTACATTGT CCGAGCATTT GGCCTGCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATCCCCTGCA CGTTGGAGCC TGTGTTTGGA AAGATGTTTG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGGT CACTCTCTAC GACTATGACC TCCTCTCCAA GGATGAAAAG 
ATTGGTGAGA CGGTCATCGA CCTGGAGAAC AGGCTGCTGT CCAAGTTTGC GGCTCGCTGT 
GGACTCCCGC AGACCTACTG TGTCTCTGGA CCGAACCAGT GGCGGGACCA GCTCCGCCCC 
TCCCAGCTCC TCCACCTCTT CTGCCAGCAG CGCAGAGTCA AGGCGCCTGT GTACCGGACA 
GACCGTGTGA TGTTTCAGGA TAAAGAATAC TCCATTGAAG AAATAGAGGC TGGCAGGATC 
CCAAACCCAC ACCTGGGCCC CGTGGAGGAG CGTCTGGCTC TGCACGTGCT TCAGCAGCAG 
GGCCTGGTCC CAGAGCACGT GGAGTCACGG CCCCTCTACA GCCCCCTGCA GCCAGACATT 
GAGCAGGGGA AGCTGCAGAT GTGGGTCGAC CTGTTTCCGA AGGCCCTGGG GCGCCCTGGA 
CCTCCCTTCA ACATCACCCC ACGGAGAGCC AGAAGGTTTT TCCTGCGTTC TATTATCTGG 
AATACAAGAG ATGTGATCCT GGATGACCTG AGCCTCACGG GGGAGAAGAT GAGCGACATT 
TATGTGAAAG GTTGGATGAT TGGCTTTGAA GAACACAAGC AAAAGACAGA CGTGCATTAT 
CGCTCCCTGG GAGGTGAAGG CAACTTCAAC TGGAGGTTCA TTTTCCCCTT CGACTACCTG 
CCGGCTGAGC AAGTCTGTAC CGTTGCCAAG AAGGATGCCT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGATATTC CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GATGATTTTC TGGGCTCCCT GCAGCTCGAT CTCAACCGCA TGCCCAAGCC AGCCAAGACA 
GCCGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCAGAATG GTTTGTGTCC 
CTTTTTGAGC AGAAAACAGT GAAGGGCTGG TGGCCTTGTG TAGCAGAAGA GGGTGAGAAG 
AAGATACTGG CGGGCAAGCT GGAAATGACC TTGGAGATTG TAACAGAGAG TGAGCATGAG 
GAGCGGCCCG CTGGCCAGGG CAGGGATGAG CCCAACATGA ACCCTAAACT TGAGGACCCA 
AGGCGCCCCG ACACCTCCTT CCTGTGGTTC ACCTCCCCAT ACAAGACCAT GAAGTTCATC 
CTGTGGCGGC GTTTCCGGTG GGCCATCATC CTCTTTATCA TCCTCTTCAT CCTGCTGCTG 
TTCCTGGCCA TCTTCATCTA CGCCTTCCCG AACTATGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832400.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688233.1 Macaca mulatta dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OMb68144
Clone ID Related Accession (Same CDS sequence)	XM_028832400.1
Accession Version	XM_028832400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAACTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGGAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAGCC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAACTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688233.1
CDS	377..6682
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832400.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832400.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAACTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGGAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAGCC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG CTTGCAGACG GTCTGTCGAG CTTGGCCCCT 
ACTAACACGG CTTCTCCTCC ATCCAGTCCT CATGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAACTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832401.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688234.1 Macaca mulatta dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OMb68145
Clone ID Related Accession (Same CDS sequence)	XM_028832401.1
Accession Version	XM_028832401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAACTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GGAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AGCCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAACTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688234.1
CDS	137..6433
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832401.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832401.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG GCGGGGGACA GAGCCGGGCC 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGACACGA GATACTCTGG AAAGAAGTGG 
CCGGCCCCCA TGGACACAGG AGGAGAGGAA GACACAGAGG ACCAGGGACT CACTGGAGAT 
GAGACAGAGC CATTCCTGGA TCAAAGCGGA GGGCCGGGGG CTCCCACCAC CCCAAGGAAA 
CTACCTTTGC ATCCTCCACC CCACTACCCC GGGATCAAAA GAAAGCGAAG TGCACCTACA 
TCTAGAAAGC TGCTGTCAGA CAAACCGCAG GACTTCCAGA TTAGGGTCCA GGTGATCGAG 
GGGCGCCAGC TGCCCGGGGT GAACATCAAG CCTGTGGTCA AGGTTACTGC TGCAGGGCAG 
ACCAAGCGGA CACGGATCCA CAAGGGAAAC AGCCCACTCT TCAATGAGAC TCTTTTCTTC 
AACTTGTTTG ACTGTCCCGC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTGGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCCTT GGGGAGTTCC GGATGGACGT GGGCACCATT 
TACAGAGAGC CCCGGCACGC CTATCTCAGG AAGTGGCTGC TGCTCTCAGA CCCTGATGAC 
TTCTCTGCTG GGGCCAGAGG TTACCTGAAA ACAAGTCTTT GTGTGCTGGG GCCTGGGGAT 
GAAGCGCCTC TGGAGAGAAA AGAGCCCTCT GAAGACAAGG AGGACATTGA AAGCAACCTG 
CTCCGGCCCA CAGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGGT CTTCCGGGCC 
GAGGACTTGC CACAGATGGA CGATGCTGTG ATGGACAACG TGAAACAGAT CTTTGGCTTT 
GAGAGTAACA AGAAGAACTT GGTGGACCCC TTTGTGGAGG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCTTGGA GAAGACGGCC AACCCTCAGT GGAACCAGAA CATCACACTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAGGATTC GTGTCATAGA CTGGGATCGC 
CTCACTCACA ATGACATCGT GGCTACCGCC GACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGACA TAGAAGTGGA TGACCACCTG GGCTTCCTCC CCACTTTTGG GCCCTGCTAC 
ATCAACCTCT ATGGCAGTCC CAGAGAGTTC ACGGGCTTCC TGGACCCCTA CGCAGAGCTC 
AACACAGGCA AGGGGGAAGG TGTGGCTTAT CGTGGCCGGC TTCTGCTTTC CCTGGAGACC 
AAGCTGGTGG AGCACAGTGA GCAGAAGGTG GAGGACCTTC CTGCGGATGA CATCCTCCGG 
GTGGAGAAGT ACCTTAGGAG GCGCAAGTAC TCCCTGTTTG CGGCCTTCTA CTCGGCCACC 
ATGCTGCAGG ATGTGGATGA CGCCATCCAG TTTGAGGTCA GCATCGGGAA CTATGGGAAC 
AAGTTCGACA TGACCTGCCT GCCGCTGGCC TCCACCACTC AGTATAGCCG TGCAGTCTTT 
GACGGGTGCC ACTACTACTA CCTACCCTGG GGCAACGTGA AACCTGTGGT GGTGCTGTCA 
TCCTACTGGG AGGACATCAG CCATAGAATC GAGACTCAGA ACCAGCTGCT TGGGATTGCT 
GACCGGCTGG AAGCTGGCCT GGAGCAGGTC CACCTGGCCC TGAAGGCGCA GTGCTCCACG 
GAGGACGTGG ACTCACTGGT GGCTCAGATG ATGGATGAGC TCATCGCAGG CTGCAGCCAG 
CCTCTGGGTG ATATCCATGA GATACCCTCT GCCACCCACC TGGACGAGTA CCTGTACCGG 
CTGCGCACCC GTCACCTGAG CCAAATCACG GAGGCTGCCC TGGCCCTGAA GCTTGGCCAC 
AGTGAGCTCC CTGCTGCTCT GGATCAGGCT GAGGACTGGC TCCTGCGTCT GCGTGCCCTC 
GCTGAGGAGC CCCAGAACAG CCTGCCGGAT ATCATCATCT GGATGCTGCA GGGAGACAAG 
CGTGTGGCAT ACCAGCGGGT GCCCGCCCAC CAAGTCCTCT TCTCCCGGCG GGGTGCCAAC 
TTCTGTGGCA AGAATTGTGG GAAGTTGCAG ACAATCTTTC TGAAATATCC GATGGAGAAG 
GTGCCTGGGG CCCGGATGCC AGTGCAGATA CGGGTCAAGC TGTGGTTTGG GCTCTCTGTG 
GATGAGAAGG AGTTCAACCA GTTTGCTGAG GGGAAGCTGT CTGTCTTTGC TGAAACCTAT 
GAGAACCAGA CCAAGTTGGC CCTGGTCGGG AACTGGGGCA CAACGGGCCT CACCTACCCC 
AAGTTTTCTG ACGTCACCGG CAAGATCAAG CTACCCAAAG AGAGCTTCCG CCCCTCGGCC 
GGCTGGACCT GGGCTGGAGA TTGGTTCGTG TGTCCGGAGA AGACCCTGCT CCATGACATG 
GACGCCGGTC ACCTGAACTT TGTGGAGGAG GTGTTTGAGA ACCAGACCCG GCTTCCCGGA 
GGCCAGTGGA TCTACATGAG TGACAACTAC ACCGATGTGA ACGGGGAGAA GGTGCTTCCC 
AAAGATGACA TTGAGTGCCC ACTGGGCTGG AAGTGGGAAG ATGAGGAATG GTCCACAGAC 
CTCAACCGGG CTGTCGATGA GCAAGGCTGG GAGTATAGCA TCACCATCCC CCCGGACCGG 
AAGCCGAAGC ACTGGGTCCC TGCTGAGAAG ATGTACTACA CACACCGACG GCGGCGCTGG 
GTGCGCCTGC GGAGGAGGGA TCTCAGCCAG ATGGAAGCGC TGAAGAGGCA TAGGCAGGCG 
GAGGCAGAGG GCGAGGGCTG GGAGTACGCC TCTCTTTTTG GCTGGAAGTT CCACCTCGAG 
TACCGCAAGA CAGACGCCTT CCGCCGCCGC CGCTGGCGCC GCCGCATGGA GCCCCTGGAG 
AAGACGGGGC CTGCAGCTGT GTTTGCCCTC GAGGGGGCCC TGGGCGGCGT GGTGGACGAC 
AAGAGTGAAG ATTCCATGTC CGTCTCCACC TTGAGCTTTG GTGTGAACAG ACCCACAATT 
TCCTGCATAT TTGACTATGG GAACCGCTAC CATCTACGCT GCTACATGTA CCAGGCCCGG 
GACCTGGCTG CGATGGACAA GGACTCTTTT TCTGATCCCT ATGCCATCGT CTCCTTCCTG 
CACCAGAGCC AGAAGACGGT GGTGGTGAAG AACACCCTTA ACCCCACCTG GGACCAGACG 
CTCATCTTCT ACGAGATCGA GATCTTCGGC GAGCCGGCCA CAGTTGCTGA GCAGCCGCCC 
AGCATTGTGG TGGAGCTGTA CGACCATGAC ACTTACGGCG CAGACGAGTT TATGGGCCGC 
TGCATCTGTC AGCCGAGTTT GGAACGGACG CCCCGACTGG CCTGGTTCCC ACTGACCAGG 
GGCAGCCAGC CGACGGGGGA GCTGCTGGCC TCTTTTGAGC TCATCCAGAG AGAGAAGCCG 
GCCATCCACC ATATTCCTGG TTTTGAGGTG CAGGAGACAT CGAGGATCCT GGATGAGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGGAGGAA GAGTTTATCG ATTGGTGGAG CAAATTCTTT 
GCCTCCATAG GGGAGAGGGA AAAGTGCGGC TCCTACTTGG AGAAGGATTT TGACACCCTG 
AAGGTCTATG ACACACAGCT GGAGAATGTG GAGGCCTTTG AGGGCCTGTC TGACTTTTGT 
AACACCTTCA AGCTGTACCG GGGCAAGACG CAGGAGGAGA CAGAAGATCC ATCTGTCATT 
GGCGAATTTA AGGGCCTCTT CAAAATTTAT CCCCTCCCAG AAGACCCAGC CATCCCCATG 
CCCCCAAGAC AGTTCCACCA GCTGGCCGCG CAGGGACCCC AGGAGTGCTT GGTCCGTATC 
TACATTGTCC GAGCATTTGG CCTGCAGCCC AAGGACCCCA ATGGGAAGTG TGATCCTTAC 
ATCAAGATCT CCATAGGGAA GAAATCAGTG AGTGACCAGG ATAACTACAT CCCCTGCACG 
TTGGAGCCTG TGTTTGGAAA GATGTTTGAG CTGACCTGCA CTCTGCCTCT GGAGAAGGAC 
CTGAAGGTCA CTCTCTACGA CTATGACCTC CTCTCCAAGG ATGAAAAGAT TGGTGAGACG 
GTCATCGACC TGGAGAACAG GCTGCTGTCC AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG 
ACCTACTGTG TCTCTGGACC GAACCAGTGG CGGGACCAGC TCCGCCCCTC CCAGCTCCTC 
CACCTCTTCT GCCAGCAGCG CAGAGTCAAG GCGCCTGTGT ACCGGACAGA CCGTGTGATG 
TTTCAGGATA AAGAATACTC CATTGAAGAA ATAGAGGCTG GCAGGATCCC AAACCCACAC 
CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG CACGTGCTTC AGCAGCAGGG CCTGGTCCCA 
GAGCACGTGG AGTCACGGCC CCTCTACAGC CCCCTGCAGC CAGACATTGA GCAGGGGAAG 
CTGCAGATGT GGGTCGACCT GTTTCCGAAG GCCCTGGGGC GCCCTGGACC TCCCTTCAAC 
ATCACCCCAC GGAGAGCCAG AAGGTTTTTC CTGCGTTCTA TTATCTGGAA TACAAGAGAT 
GTGATCCTGG ATGACCTGAG CCTCACGGGG GAGAAGATGA GCGACATTTA TGTGAAAGGT 
TGGATGATTG GCTTTGAAGA ACACAAGCAA AAGACAGACG TGCATTATCG CTCCCTGGGA 
GGTGAAGGCA ACTTCAACTG GAGGTTCATT TTCCCCTTCG ACTACCTGCC GGCTGAGCAA 
GTCTGTACCG TTGCCAAGAA GGATGCCTTC TGGAGGCTGG ACAAGACTGA GAGCAAAATC 
CCAGCACGAG TGATATTCCA GATCTGGGAC AATGACAAGT TCTCCTTTGA TGATTTTCTG 
GGCTCCCTGC AGCTCGATCT CAACCGCATG CCCAAGCCAG CCAAGACAGC CGAGAAGTGC 
TCCTTGGACC AGCTGGATGA CACTTTCCAC CCAGAATGGT TTGTGTCCCT TTTTGAGCAG 
AAAACAGTGA AGGGCTGGTG GCCTTGTGTA GCAGAAGAGG GTGAGAAGAA GATACTGGCG 
GGCAAGCTGG AAATGACCTT GGAGATTGTA ACAGAGAGTG AGCATGAGGA GCGGCCCGCT 
GGCCAGGGCA GGGATGAGCC CAACATGAAC CCTAAACTTG AGGACCCAAG GCGCCCCGAC 
ACCTCCTTCC TGTGGTTCAC CTCCCCATAC AAGACCATGA AGTTCATCCT GTGGCGGCGT 
TTCCGGTGGG CCATCATCCT CTTTATCATC CTCTTCATCC TGCTGCTGTT CCTGGCCATC 
TTCATCTACG CCTTCCCGAA CTATGCTGCC ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832402.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688235.1 Macaca mulatta dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OMb68146
Clone ID Related Accession (Same CDS sequence)	XM_028832402.1
Accession Version	XM_028832402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAACTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGGA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAGC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCGCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGGTCACTC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCGCAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGCC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTCTATTA TCTGGAATAC AAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACCGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCAGGG ATGAGCCCAA CATGAACCCT AAACTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT TATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688235.1
CDS	376..6669
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832402.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832402.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGGCG GGGGACAGAG CCGGGCCGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GACACGAGAT ACTCTGGAAA GAAGTGGCCG 
GCCCCCATGG ACACAGGAGG AGAGGAAGAC ACAGAGGACC AGGGACTCAC TGGAGATGAG 
ACAGAGCCAT TCCTGGATCA AAGCGGAGGG CCGGGGGCTC CCACCACCCC AAGGAAACTA 
CCTTTGCATC CTCCACCCCA CTACCCCGGG ATCAAAAGAA AGCGAAGTGC ACCTACATCT 
AGAAAGCTGC TGTCAGACAA ACCGCAGGAC TTCCAGATTA GGGTCCAGGT GATCGAGGGG 
CGCCAGCTGC CCGGGGTGAA CATCAAGCCT GTGGTCAAGG TTACTGCTGC AGGGCAGACC 
AAGCGGACAC GGATCCACAA GGGAAACAGC CCACTCTTCA ATGAGACTCT TTTCTTCAAC 
TTGTTTGACT GTCCCGCGGA GCTGTTTGAT GAGCCCATCT TTATCACGGT GGTAGACTCC 
CGTTCTCTCA GGACAGATGC TCTCCTTGGG GAGTTCCGGA TGGACGTGGG CACCATTTAC 
AGAGAGCCCC GGCACGCCTA TCTCAGGAAG TGGCTGCTGC TCTCAGACCC TGATGACTTC 
TCTGCTGGGG CCAGAGGTTA CCTGAAAACA AGTCTTTGTG TGCTGGGGCC TGGGGATGAA 
GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA GACAAGGAGG ACATTGAAAG CAACCTGCTC 
CGGCCCACAG GCGTGGCCCT GCGAGGAGCC CACTTCTGCC TGAAGGTCTT CCGGGCCGAG 
GACTTGCCAC AGATGGACGA TGCTGTGATG GACAACGTGA AACAGATCTT TGGCTTTGAG 
AGTAACAAGA AGAACTTGGT GGACCCCTTT GTGGAGGTCA GCTTTGCGGG GAAAATGCTC 
TGCAGCAAGA TCTTGGAGAA GACGGCCAAC CCTCAGTGGA ACCAGAACAT CACACTGCCT 
GCCATGTTTC CCTCCATGTG TGAAAAAATG AGGATTCGTG TCATAGACTG GGATCGCCTC 
ACTCACAATG ACATCGTGGC TACCGCCGAC CTGAGTATGT CGAAAATCTC TGCCCCTGGA 
GGAGACATAG AAGTGGATGA CCACCTGGGC TTCCTCCCCA CTTTTGGGCC CTGCTACATC 
AACCTCTATG GCAGTCCCAG AGAGTTCACG GGCTTCCTGG ACCCCTACGC AGAGCTCAAC 
ACAGGCAAGG GGGAAGGTGT GGCTTATCGT GGCCGGCTTC TGCTTTCCCT GGAGACCAAG 
CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG GACCTTCCTG CGGATGACAT CCTCCGGGTG 
GAGAAGTACC TTAGGAGGCG CAAGTACTCC CTGTTTGCGG CCTTCTACTC GGCCACCATG 
CTGCAGGATG TGGATGACGC CATCCAGTTT GAGGTCAGCA TCGGGAACTA TGGGAACAAG 
TTCGACATGA CCTGCCTGCC GCTGGCCTCC ACCACTCAGT ATAGCCGTGC AGTCTTTGAC 
GGGTGCCACT ACTACTACCT ACCCTGGGGC AACGTGAAAC CTGTGGTGGT GCTGTCATCC 
TACTGGGAGG ACATCAGCCA TAGAATCGAG ACTCAGAACC AGCTGCTTGG GATTGCTGAC 
CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC CTGGCCCTGA AGGCGCAGTG CTCCACGGAG 
GACGTGGACT CACTGGTGGC TCAGATGATG GATGAGCTCA TCGCAGGCTG CAGCCAGCCT 
CTGGGTGATA TCCATGAGAT ACCCTCTGCC ACCCACCTGG ACGAGTACCT GTACCGGCTG 
CGCACCCGTC ACCTGAGCCA AATCACGGAG GCTGCCCTGG CCCTGAAGCT TGGCCACAGT 
GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG GACTGGCTCC TGCGTCTGCG TGCCCTCGCT 
GAGGAGCCCC AGAACAGCCT GCCGGATATC ATCATCTGGA TGCTGCAGGG AGACAAGCGT 
GTGGCATACC AGCGGGTGCC CGCCCACCAA GTCCTCTTCT CCCGGCGGGG TGCCAACTTC 
TGTGGCAAGA ATTGTGGGAA GTTGCAGACA ATCTTTCTGA AATATCCGAT GGAGAAGGTG 
CCTGGGGCCC GGATGCCAGT GCAGATACGG GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT 
GAGAAGGAGT TCAACCAGTT TGCTGAGGGG AAGCTGTCTG TCTTTGCTGA AACCTATGAG 
AACCAGACCA AGTTGGCCCT GGTCGGGAAC TGGGGCACAA CGGGCCTCAC CTACCCCAAG 
TTTTCTGACG TCACCGGCAA GATCAAGCTA CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC 
TGGACCTGGG CTGGAGATTG GTTCGTGTGT CCGGAGAAGA CCCTGCTCCA TGACATGGAC 
GCCGGTCACC TGAACTTTGT GGAGGAGGTG TTTGAGAACC AGACCCGGCT TCCCGGAGGC 
CAGTGGATCT ACATGAGTGA CAACTACACC GATGTGAACG GGGAGAAGGT GCTTCCCAAA 
GATGACATTG AGTGCCCACT GGGCTGGAAG TGGGAAGATG AGGAATGGTC CACAGACCTC 
AACCGGGCTG TCGATGAGCA AGGCTGGGAG TATAGCATCA CCATCCCCCC GGACCGGAAG 
CCGAAGCACT GGGTCCCTGC TGAGAAGATG TACTACACAC ACCGACGGCG GCGCTGGGTG 
CGCCTGCGGA GGAGGGATCT CAGCCAGATG GAAGCGCTGA AGAGGCATAG GCAGGCGGAG 
GCAGAGGGCG AGGGCTGGGA GTACGCCTCT CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC 
CGCAAGACAG ACGCCTTCCG CCGCCGCCGC TGGCGCCGCC GCATGGAGCC CCTGGAGAAG 
ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG GGGGCCCTGG GCGGCGTGGT GGACGACAAG 
AGTGAAGATT CCATGTCCGT CTCCACCTTG AGCTTTGGTG TGAACAGACC CACAATTTCC 
TGCATATTTG ACTATGGGAA CCGCTACCAT CTACGCTGCT ACATGTACCA GGCCCGGGAC 
CTGGCTGCGA TGGACAAGGA CTCTTTTTCT GATCCCTATG CCATCGTCTC CTTCCTGCAC 
CAGAGCCAGA AGACGGTGGT GGTGAAGAAC ACCCTTAACC CCACCTGGGA CCAGACGCTC 
ATCTTCTACG AGATCGAGAT CTTCGGCGAG CCGGCCACAG TTGCTGAGCA GCCGCCCAGC 
ATTGTGGTGG AGCTGTACGA CCATGACACT TACGGCGCAG ACGAGTTTAT GGGCCGCTGC 
ATCTGTCAGC CGAGTTTGGA ACGGACGCCC CGACTGGCCT GGTTCCCACT GACCAGGGGC 
AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC 
ATCCACCATA TTCCTGGTTT TGAGGTGCAG GAGACATCGA GGATCCTGGA TGAGTCTGAG 
GACACAGACC TGCCCTACCC ACCACCCCAG AGGGAGGCCA ACATCTACAT GGTTCCTCAG 
AACATCAAGC CAGCGCTCCA GCGTACCGCC ATCGAGGTCC TGGCATGGGG CCTGCGGAAC 
ATGAAGAGTT ACCAGCTGGC CAGCATCTCC TCCCCGAGCC TTGTGATAGA GTGTGGGGGC 
CAGACGGTGC AGTCCTGTGT CATCAGGAAC CTCCGGAAGA ACCCCAACTT TGACATCTGT 
ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG GAGGAGCTCT ACTGCCCCCC CATCACCATC 
AAGGTCATCG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGC 
TCCCTGGAGA GCTTCCTGTG TGACCCCTAC TCGGCGGAGA GTCCATCCCC ACAGGGTGGC 
CCAGACGATG TGAGCCTACT CAGTCCTGGG GAAGACGTGC TCATCGACAT TGATGACAAG 
GAGCCCCTCA TCCCCATCCA GGAGGAAGAG TTTATCGATT GGTGGAGCAA ATTCTTTGCC 
TCCATAGGGG AGAGGGAAAA GTGCGGCTCC TACTTGGAGA AGGATTTTGA CACCCTGAAG 
GTCTATGACA CACAGCTGGA GAATGTGGAG GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC 
ACCTTCAAGC TGTACCGGGG CAAGACGCAG GAGGAGACAG AAGATCCATC TGTCATTGGC 
GAATTTAAGG GCCTCTTCAA AATTTATCCC CTCCCAGAAG ACCCAGCCAT CCCCATGCCC 
CCAAGACAGT TCCACCAGCT GGCCGCGCAG GGACCCCAGG AGTGCTTGGT CCGTATCTAC 
ATTGTCCGAG CATTTGGCCT GCAGCCCAAG GACCCCAATG GGAAGTGTGA TCCTTACATC 
AAGATCTCCA TAGGGAAGAA ATCAGTGAGT GACCAGGATA ACTACATCCC CTGCACGTTG 
GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG ACCTGCACTC TGCCTCTGGA GAAGGACCTG 
AAGGTCACTC TCTACGACTA TGACCTCCTC TCCAAGGATG AAAAGATTGG TGAGACGGTC 
ATCGACCTGG AGAACAGGCT GCTGTCCAAG TTTGCGGCTC GCTGTGGACT CCCGCAGACC 
TACTGTGTCT CTGGACCGAA CCAGTGGCGG GACCAGCTCC GCCCCTCCCA GCTCCTCCAC 
CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG CCTGTGTACC GGACAGACCG TGTGATGTTT 
CAGGATAAAG AATACTCCAT TGAAGAAATA GAGGCTGGCA GGATCCCAAA CCCACACCTG 
GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG 
CACGTGGAGT CACGGCCCCT CTACAGCCCC CTGCAGCCAG ACATTGAGCA GGGGAAGCTG 
CAGATGTGGG TCGACCTGTT TCCGAAGGCC CTGGGGCGCC CTGGACCTCC CTTCAACATC 
ACCCCACGGA GAGCCAGAAG GTTTTTCCTG CGTTCTATTA TCTGGAATAC AAGAGATGTG 
ATCCTGGATG ACCTGAGCCT CACGGGGGAG AAGATGAGCG ACATTTATGT GAAAGGTTGG 
ATGATTGGCT TTGAAGAACA CAAGCAAAAG ACAGACGTGC ATTATCGCTC CCTGGGAGGT 
GAAGGCAACT TCAACTGGAG GTTCATTTTC CCCTTCGACT ACCTGCCGGC TGAGCAAGTC 
TGTACCGTTG CCAAGAAGGA TGCCTTCTGG AGGCTGGACA AGACTGAGAG CAAAATCCCA 
GCACGAGTGA TATTCCAGAT CTGGGACAAT GACAAGTTCT CCTTTGATGA TTTTCTGGGC 
TCCCTGCAGC TCGATCTCAA CCGCATGCCC AAGCCAGCCA AGACAGCCGA GAAGTGCTCC 
TTGGACCAGC TGGATGACAC TTTCCACCCA GAATGGTTTG TGTCCCTTTT TGAGCAGAAA 
ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC 
AAGCTGGAAA TGACCTTGGA GATTGTAACA GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC 
CAGGGCAGGG ATGAGCCCAA CATGAACCCT AAACTTGAGG ACCCAAGGCG CCCCGACACC 
TCCTTCCTGT GGTTCACCTC CCCATACAAG ACCATGAAGT TCATCCTGTG GCGGCGTTTC 
CGGTGGGCCA TCATCCTCTT TATCATCCTC TTCATCCTGC TGCTGTTCCT GGCCATCTTC 
ATCTACGCCT TCCCGAACTA TGCTGCCATG AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832403.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688236.1 Macaca mulatta dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	OMb68147
Clone ID Related Accession (Same CDS sequence)	XM_028832403.1
Accession Version	XM_028832403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAACTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGGA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAGC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCG 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CTCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG 
CACGTGCTTC AGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GCCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTCTA TTATCTGGAA TACAAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACCG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCTTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCA GGGATGAGCC CAACATGAAC 
CCTAAACTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTTATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688236.1
CDS	137..6403
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832403.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832403.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAACTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGGA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAGC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GCTTGCAGAC 
GGTCTGTCGA GCTTGGCCCC TACTAACACG GCTTCTCCTC CATCCAGTCC TCATGAGGAA 
GAGTTTATCG ATTGGTGGAG CAAATTCTTT GCCTCCATAG GGGAGAGGGA AAAGTGCGGC 
TCCTACTTGG AGAAGGATTT TGACACCCTG AAGGTCTATG ACACACAGCT GGAGAATGTG 
GAGGCCTTTG AGGGCCTGTC TGACTTTTGT AACACCTTCA AGCTGTACCG GGGCAAGACG 
CAGGAGGAGA CAGAAGATCC ATCTGTCATT GGCGAATTTA AGGGCCTCTT CAAAATTTAT 
CCCCTCCCAG AAGACCCAGC CATCCCCATG CCCCCAAGAC AGTTCCACCA GCTGGCCGCG 
CAGGGACCCC AGGAGTGCTT GGTCCGTATC TACATTGTCC GAGCATTTGG CCTGCAGCCC 
AAGGACCCCA ATGGGAAGTG TGATCCTTAC ATCAAGATCT CCATAGGGAA GAAATCAGTG 
AGTGACCAGG ATAACTACAT CCCCTGCACG TTGGAGCCTG TGTTTGGAAA GATGTTTGAG 
CTGACCTGCA CTCTGCCTCT GGAGAAGGAC CTGAAGGTCA CTCTCTACGA CTATGACCTC 
CTCTCCAAGG ATGAAAAGAT TGGTGAGACG GTCATCGACC TGGAGAACAG GCTGCTGTCC 
AAGTTTGCGG CTCGCTGTGG ACTCCCGCAG ACCTACTGTG TCTCTGGACC GAACCAGTGG 
CGGGACCAGC TCCGCCCCTC CCAGCTCCTC CACCTCTTCT GCCAGCAGCG CAGAGTCAAG 
GCGCCTGTGT ACCGGACAGA CCGTGTGATG TTTCAGGATA AAGAATACTC CATTGAAGAA 
ATAGAGGCTG GCAGGATCCC AAACCCACAC CTGGGCCCCG TGGAGGAGCG TCTGGCTCTG 
CACGTGCTTC AGCAGCAGGG CCTGGTCCCA GAGCACGTGG AGTCACGGCC CCTCTACAGC 
CCCCTGCAGC CAGACATTGA GCAGGGGAAG CTGCAGATGT GGGTCGACCT GTTTCCGAAG 
GCCCTGGGGC GCCCTGGACC TCCCTTCAAC ATCACCCCAC GGAGAGCCAG AAGGTTTTTC 
CTGCGTTCTA TTATCTGGAA TACAAGAGAT GTGATCCTGG ATGACCTGAG CCTCACGGGG 
GAGAAGATGA GCGACATTTA TGTGAAAGGT TGGATGATTG GCTTTGAAGA ACACAAGCAA 
AAGACAGACG TGCATTATCG CTCCCTGGGA GGTGAAGGCA ACTTCAACTG GAGGTTCATT 
TTCCCCTTCG ACTACCTGCC GGCTGAGCAA GTCTGTACCG TTGCCAAGAA GGATGCCTTC 
TGGAGGCTGG ACAAGACTGA GAGCAAAATC CCAGCACGAG TGATATTCCA GATCTGGGAC 
AATGACAAGT TCTCCTTTGA TGATTTTCTG GGCTCCCTGC AGCTCGATCT CAACCGCATG 
CCCAAGCCAG CCAAGACAGC CGAGAAGTGC TCCTTGGACC AGCTGGATGA CACTTTCCAC 
CCAGAATGGT TTGTGTCCCT TTTTGAGCAG AAAACAGTGA AGGGCTGGTG GCCTTGTGTA 
GCAGAAGAGG GTGAGAAGAA GATACTGGCG GGCAAGCTGG AAATGACCTT GGAGATTGTA 
ACAGAGAGTG AGCATGAGGA GCGGCCCGCT GGCCAGGGCA GGGATGAGCC CAACATGAAC 
CCTAAACTTG AGGACCCAAG GCGCCCCGAC ACCTCCTTCC TGTGGTTCAC CTCCCCATAC 
AAGACCATGA AGTTCATCCT GTGGCGGCGT TTCCGGTGGG CCATCATCCT CTTTATCATC 
CTCTTCATCC TGCTGCTGTT CCTGGCCATC TTCATCTACG CCTTCCCGAA CTATGCTGCC 
ATGAAGCTGG TGAAGCCCTT CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832404.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688237.1 Macaca mulatta dysferlin (DYSF), transcript variant X17, mRNA.

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CloneID	OMb68148
Clone ID Related Accession (Same CDS sequence)	XM_028832404.1
Accession Version	XM_028832404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA ACTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGGAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAGCCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAACTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688237.1
CDS	376..6639
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832404.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832404.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA ACTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGGAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAGCCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGCT TGCAGACGGT 
CTGTCGAGCT TGGCCCCTAC TAACACGGCT TCTCCTCCAT CCAGTCCTCA TGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAACTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832405.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688238.1 Macaca mulatta dysferlin (DYSF), transcript variant X18, mRNA.

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CloneID	OMb68149
Clone ID Related Accession (Same CDS sequence)	XM_028832405.1
Accession Version	XM_028832405.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAACTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA GCCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAACTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688238.1
CDS	136..6381
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832405.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832405.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGAGGAGAC TGCAGGTGCC 
GTCAAGCCTT CGAAAGTCTC AGACCTGGAT GACCACCTGG GCTTCCTCCC CACTTTTGGG 
CCCTGCTACA TCAACCTCTA TGGCAGTCCC AGAGAGTTCA CGGGCTTCCT GGACCCCTAC 
GCAGAGCTCA ACACAGGCAA GGGGGAAGGT GTGGCTTATC GTGGCCGGCT TCTGCTTTCC 
CTGGAGACCA AGCTGGTGGA GCACAGTGAG CAGAAGGTGG AGGACCTTCC TGCGGATGAC 
ATCCTCCGGG TGGAGAAGTA CCTTAGGAGG CGCAAGTACT CCCTGTTTGC GGCCTTCTAC 
TCGGCCACCA TGCTGCAGGA TGTGGATGAC GCCATCCAGT TTGAGGTCAG CATCGGGAAC 
TATGGGAACA AGTTCGACAT GACCTGCCTG CCGCTGGCCT CCACCACTCA GTATAGCCGT 
GCAGTCTTTG ACGGGTGCCA CTACTACTAC CTACCCTGGG GCAACGTGAA ACCTGTGGTG 
GTGCTGTCAT CCTACTGGGA GGACATCAGC CATAGAATCG AGACTCAGAA CCAGCTGCTT 
GGGATTGCTG ACCGGCTGGA AGCTGGCCTG GAGCAGGTCC ACCTGGCCCT GAAGGCGCAG 
TGCTCCACGG AGGACGTGGA CTCACTGGTG GCTCAGATGA TGGATGAGCT CATCGCAGGC 
TGCAGCCAGC CTCTGGGTGA TATCCATGAG ATACCCTCTG CCACCCACCT GGACGAGTAC 
CTGTACCGGC TGCGCACCCG TCACCTGAGC CAAATCACGG AGGCTGCCCT GGCCCTGAAG 
CTTGGCCACA GTGAGCTCCC TGCTGCTCTG GATCAGGCTG AGGACTGGCT CCTGCGTCTG 
CGTGCCCTCG CTGAGGAGCC CCAGAACAGC CTGCCGGATA TCATCATCTG GATGCTGCAG 
GGAGACAAGC GTGTGGCATA CCAGCGGGTG CCCGCCCACC AAGTCCTCTT CTCCCGGCGG 
GGTGCCAACT TCTGTGGCAA GAATTGTGGG AAGTTGCAGA CAATCTTTCT GAAATATCCG 
ATGGAGAAGG TGCCTGGGGC CCGGATGCCA GTGCAGATAC GGGTCAAGCT GTGGTTTGGG 
CTCTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GGAAGCTGTC TGTCTTTGCT 
GAAACCTATG AGAACCAGAC CAAGTTGGCC CTGGTCGGGA ACTGGGGCAC AACGGGCCTC 
ACCTACCCCA AGTTTTCTGA CGTCACCGGC AAGATCAAGC TACCCAAAGA GAGCTTCCGC 
CCCTCGGCCG GCTGGACCTG GGCTGGAGAT TGGTTCGTGT GTCCGGAGAA GACCCTGCTC 
CATGACATGG ACGCCGGTCA CCTGAACTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTTCCCGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CCGATGTGAA CGGGGAGAAG 
GTGCTTCCCA AAGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAGGAATGG 
TCCACAGACC TCAACCGGGC TGTCGATGAG CAAGGCTGGG AGTATAGCAT CACCATCCCC 
CCGGACCGGA AGCCGAAGCA CTGGGTCCCT GCTGAGAAGA TGTACTACAC ACACCGACGG 
CGGCGCTGGG TGCGCCTGCG GAGGAGGGAT CTCAGCCAGA TGGAAGCGCT GAAGAGGCAT 
AGGCAGGCGG AGGCAGAGGG CGAGGGCTGG GAGTACGCCT CTCTTTTTGG CTGGAAGTTC 
CACCTCGAGT ACCGCAAGAC AGACGCCTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAG 
CCCCTGGAGA AGACGGGGCC TGCAGCTGTG TTTGCCCTCG AGGGGGCCCT GGGCGGCGTG 
GTGGACGACA AGAGTGAAGA TTCCATGTCC GTCTCCACCT TGAGCTTTGG TGTGAACAGA 
CCCACAATTT CCTGCATATT TGACTATGGG AACCGCTACC ATCTACGCTG CTACATGTAC 
CAGGCCCGGG ACCTGGCTGC GATGGACAAG GACTCTTTTT CTGATCCCTA TGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACGGTG GTGGTGAAGA ACACCCTTAA CCCCACCTGG 
GACCAGACGC TCATCTTCTA CGAGATCGAG ATCTTCGGCG AGCCGGCCAC AGTTGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAC GACCATGACA CTTACGGCGC AGACGAGTTT 
ATGGGCCGCT GCATCTGTCA GCCGAGTTTG GAACGGACGC CCCGACTGGC CTGGTTCCCA 
CTGACCAGGG GCAGCCAGCC GACGGGGGAG CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAGAAGCCGG CCATCCACCA TATTCCTGGT TTTGAGGTGC AGGAGACATC GAGGATCCTG 
GATGAGTCTG AGGACACAGA CCTGCCCTAC CCACCACCCC AGAGGGAGGC CAACATCTAC 
ATGGTTCCTC AGAACATCAA GCCAGCGCTC CAGCGTACCG CCATCGAGGT CCTGGCATGG 
GGCCTGCGGA ACATGAAGAG TTACCAGCTG GCCAGCATCT CCTCCCCGAG CCTTGTGATA 
GAGTGTGGGG GCCAGACGGT GCAGTCCTGT GTCATCAGGA ACCTCCGGAA GAACCCCAAC 
TTTGACATCT GTACCCTCTT CATGGAAGTG ATGCTGCCCA GGGAGGAGCT CTACTGCCCC 
CCCATCACCA TCAAGGTCAT CGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GCTCCCTGGA GAGCTTCCTG TGTGACCCCT ACTCGGCGGA GAGTCCATCC 
CCACAGGGTG GCCCAGACGA TGTGAGCCTA CTCAGTCCTG GGGAAGACGT GCTCATCGAC 
ATTGATGACA AGGAGCCCCT CATCCCCATC CAGGAGGAAG AGTTTATCGA TTGGTGGAGC 
AAATTCTTTG CCTCCATAGG GGAGAGGGAA AAGTGCGGCT CCTACTTGGA GAAGGATTTT 
GACACCCTGA AGGTCTATGA CACACAGCTG GAGAATGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTGTACCGG GGCAAGACGC AGGAGGAGAC AGAAGATCCA 
TCTGTCATTG GCGAATTTAA GGGCCTCTTC AAAATTTATC CCCTCCCAGA AGACCCAGCC 
ATCCCCATGC CCCCAAGACA GTTCCACCAG CTGGCCGCGC AGGGACCCCA GGAGTGCTTG 
GTCCGTATCT ACATTGTCCG AGCATTTGGC CTGCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATC 
CCCTGCACGT TGGAGCCTGT GTTTGGAAAG ATGTTTGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGGTCAC TCTCTACGAC TATGACCTCC TCTCCAAGGA TGAAAAGATT 
GGTGAGACGG TCATCGACCT GGAGAACAGG CTGCTGTCCA AGTTTGCGGC TCGCTGTGGA 
CTCCCGCAGA CCTACTGTGT CTCTGGACCG AACCAGTGGC GGGACCAGCT CCGCCCCTCC 
CAGCTCCTCC ACCTCTTCTG CCAGCAGCGC AGAGTCAAGG CGCCTGTGTA CCGGACAGAC 
CGTGTGATGT TTCAGGATAA AGAATACTCC ATTGAAGAAA TAGAGGCTGG CAGGATCCCA 
AACCCACACC TGGGCCCCGT GGAGGAGCGT CTGGCTCTGC ACGTGCTTCA GCAGCAGGGC 
CTGGTCCCAG AGCACGTGGA GTCACGGCCC CTCTACAGCC CCCTGCAGCC AGACATTGAG 
CAGGGGAAGC TGCAGATGTG GGTCGACCTG TTTCCGAAGG CCCTGGGGCG CCCTGGACCT 
CCCTTCAACA TCACCCCACG GAGAGCCAGA AGGTTTTTCC TGCGTTCTAT TATCTGGAAT 
ACAAGAGATG TGATCCTGGA TGACCTGAGC CTCACGGGGG AGAAGATGAG CGACATTTAT 
GTGAAAGGTT GGATGATTGG CTTTGAAGAA CACAAGCAAA AGACAGACGT GCATTATCGC 
TCCCTGGGAG GTGAAGGCAA CTTCAACTGG AGGTTCATTT TCCCCTTCGA CTACCTGCCG 
GCTGAGCAAG TCTGTACCGT TGCCAAGAAG GATGCCTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GATATTCCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAT 
GATTTTCTGG GCTCCCTGCA GCTCGATCTC AACCGCATGC CCAAGCCAGC CAAGACAGCC 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CAGAATGGTT TGTGTCCCTT 
TTTGAGCAGA AAACAGTGAA GGGCTGGTGG CCTTGTGTAG CAGAAGAGGG TGAGAAGAAG 
ATACTGGCGG GCAAGCTGGA AATGACCTTG GAGATTGTAA CAGAGAGTGA GCATGAGGAG 
CGGCCCGCTG GCCAGGGCAG GGATGAGCCC AACATGAACC CTAAACTTGA GGACCCAAGG 
CGCCCCGACA CCTCCTTCCT GTGGTTCACC TCCCCATACA AGACCATGAA GTTCATCCTG 
TGGCGGCGTT TCCGGTGGGC CATCATCCTC TTTATCATCC TCTTCATCCT GCTGCTGTTC 
CTGGCCATCT TCATCTACGC CTTCCCGAAC TATGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832406.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688239.1 Macaca mulatta dysferlin (DYSF), transcript variant X19, mRNA.

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CloneID	OMb68150
Clone ID Related Accession (Same CDS sequence)	XM_028832406.1
Accession Version	XM_028832406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAACTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGGAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAGCC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCGCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACTCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCGCAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCGTGGA GGAGCGTCTG GCTCTGCACG TGCTTCAGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGCCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTCTATTAT CTGGAATACA 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACCGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCT TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCAGGGA TGAGCCCAAC ATGAACCCTA AACTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTT ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688239.1
CDS	377..6619
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832406.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832406.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG AGGAGACTGC AGGTGCCGTC 
AAGCCTTCGA AAGTCTCAGA CCTGGATGAC CACCTGGGCT TCCTCCCCAC TTTTGGGCCC 
TGCTACATCA ACCTCTATGG CAGTCCCAGA GAGTTCACGG GCTTCCTGGA CCCCTACGCA 
GAGCTCAACA CAGGCAAGGG GGAAGGTGTG GCTTATCGTG GCCGGCTTCT GCTTTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGTGAGCAG AAGGTGGAGG ACCTTCCTGC GGATGACATC 
CTCCGGGTGG AGAAGTACCT TAGGAGGCGC AAGTACTCCC TGTTTGCGGC CTTCTACTCG 
GCCACCATGC TGCAGGATGT GGATGACGCC ATCCAGTTTG AGGTCAGCAT CGGGAACTAT 
GGGAACAAGT TCGACATGAC CTGCCTGCCG CTGGCCTCCA CCACTCAGTA TAGCCGTGCA 
GTCTTTGACG GGTGCCACTA CTACTACCTA CCCTGGGGCA ACGTGAAACC TGTGGTGGTG 
CTGTCATCCT ACTGGGAGGA CATCAGCCAT AGAATCGAGA CTCAGAACCA GCTGCTTGGG 
ATTGCTGACC GGCTGGAAGC TGGCCTGGAG CAGGTCCACC TGGCCCTGAA GGCGCAGTGC 
TCCACGGAGG ACGTGGACTC ACTGGTGGCT CAGATGATGG ATGAGCTCAT CGCAGGCTGC 
AGCCAGCCTC TGGGTGATAT CCATGAGATA CCCTCTGCCA CCCACCTGGA CGAGTACCTG 
TACCGGCTGC GCACCCGTCA CCTGAGCCAA ATCACGGAGG CTGCCCTGGC CCTGAAGCTT 
GGCCACAGTG AGCTCCCTGC TGCTCTGGAT CAGGCTGAGG ACTGGCTCCT GCGTCTGCGT 
GCCCTCGCTG AGGAGCCCCA GAACAGCCTG CCGGATATCA TCATCTGGAT GCTGCAGGGA 
GACAAGCGTG TGGCATACCA GCGGGTGCCC GCCCACCAAG TCCTCTTCTC CCGGCGGGGT 
GCCAACTTCT GTGGCAAGAA TTGTGGGAAG TTGCAGACAA TCTTTCTGAA ATATCCGATG 
GAGAAGGTGC CTGGGGCCCG GATGCCAGTG CAGATACGGG TCAAGCTGTG GTTTGGGCTC 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGGA AGCTGTCTGT CTTTGCTGAA 
ACCTATGAGA ACCAGACCAA GTTGGCCCTG GTCGGGAACT GGGGCACAAC GGGCCTCACC 
TACCCCAAGT TTTCTGACGT CACCGGCAAG ATCAAGCTAC CCAAAGAGAG CTTCCGCCCC 
TCGGCCGGCT GGACCTGGGC TGGAGATTGG TTCGTGTGTC CGGAGAAGAC CCTGCTCCAT 
GACATGGACG CCGGTCACCT GAACTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTT 
CCCGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACCG ATGTGAACGG GGAGAAGGTG 
CTTCCCAAAG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA GGAATGGTCC 
ACAGACCTCA ACCGGGCTGT CGATGAGCAA GGCTGGGAGT ATAGCATCAC CATCCCCCCG 
GACCGGAAGC CGAAGCACTG GGTCCCTGCT GAGAAGATGT ACTACACACA CCGACGGCGG 
CGCTGGGTGC GCCTGCGGAG GAGGGATCTC AGCCAGATGG AAGCGCTGAA GAGGCATAGG 
CAGGCGGAGG CAGAGGGCGA GGGCTGGGAG TACGCCTCTC TTTTTGGCTG GAAGTTCCAC 
CTCGAGTACC GCAAGACAGA CGCCTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAGCCC 
CTGGAGAAGA CGGGGCCTGC AGCTGTGTTT GCCCTCGAGG GGGCCCTGGG CGGCGTGGTG 
GACGACAAGA GTGAAGATTC CATGTCCGTC TCCACCTTGA GCTTTGGTGT GAACAGACCC 
ACAATTTCCT GCATATTTGA CTATGGGAAC CGCTACCATC TACGCTGCTA CATGTACCAG 
GCCCGGGACC TGGCTGCGAT GGACAAGGAC TCTTTTTCTG ATCCCTATGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACGGTGGTG GTGAAGAACA CCCTTAACCC CACCTGGGAC 
CAGACGCTCA TCTTCTACGA GATCGAGATC TTCGGCGAGC CGGCCACAGT TGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTACGAC CATGACACTT ACGGCGCAGA CGAGTTTATG 
GGCCGCTGCA TCTGTCAGCC GAGTTTGGAA CGGACGCCCC GACTGGCCTG GTTCCCACTG 
ACCAGGGGCA GCCAGCCGAC GGGGGAGCTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAG 
AAGCCGGCCA TCCACCATAT TCCTGGTTTT GAGGTGCAGG AGACATCGAG GATCCTGGAT 
GAGTCTGAGG ACACAGACCT GCCCTACCCA CCACCCCAGA GGGAGGCCAA CATCTACATG 
GTTCCTCAGA ACATCAAGCC AGCGCTCCAG CGTACCGCCA TCGAGGTCCT GGCATGGGGC 
CTGCGGAACA TGAAGAGTTA CCAGCTGGCC AGCATCTCCT CCCCGAGCCT TGTGATAGAG 
TGTGGGGGCC AGACGGTGCA GTCCTGTGTC ATCAGGAACC TCCGGAAGAA CCCCAACTTT 
GACATCTGTA CCCTCTTCAT GGAAGTGATG CTGCCCAGGG AGGAGCTCTA CTGCCCCCCC 
ATCACCATCA AGGTCATCGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGCT CCCTGGAGAG CTTCCTGTGT GACCCCTACT CGGCGGAGAG TCCATCCCCA 
CAGGGTGGCC CAGACGATGT GAGCCTACTC AGTCCTGGGG AAGACGTGCT CATCGACATT 
GATGACAAGG AGCCCCTCAT CCCCATCCAG GAGGAAGAGT TTATCGATTG GTGGAGCAAA 
TTCTTTGCCT CCATAGGGGA GAGGGAAAAG TGCGGCTCCT ACTTGGAGAA GGATTTTGAC 
ACCCTGAAGG TCTATGACAC ACAGCTGGAG AATGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT GTACCGGGGC AAGACGCAGG AGGAGACAGA AGATCCATCT 
GTCATTGGCG AATTTAAGGG CCTCTTCAAA ATTTATCCCC TCCCAGAAGA CCCAGCCATC 
CCCATGCCCC CAAGACAGTT CCACCAGCTG GCCGCGCAGG GACCCCAGGA GTGCTTGGTC 
CGTATCTACA TTGTCCGAGC ATTTGGCCTG CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATCCCC 
TGCACGTTGG AGCCTGTGTT TGGAAAGATG TTTGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGGTCACTCT CTACGACTAT GACCTCCTCT CCAAGGATGA AAAGATTGGT 
GAGACGGTCA TCGACCTGGA GAACAGGCTG CTGTCCAAGT TTGCGGCTCG CTGTGGACTC 
CCGCAGACCT ACTGTGTCTC TGGACCGAAC CAGTGGCGGG ACCAGCTCCG CCCCTCCCAG 
CTCCTCCACC TCTTCTGCCA GCAGCGCAGA GTCAAGGCGC CTGTGTACCG GACAGACCGT 
GTGATGTTTC AGGATAAAGA ATACTCCATT GAAGAAATAG AGGCTGGCAG GATCCCAAAC 
CCACACCTGG GCCCCGTGGA GGAGCGTCTG GCTCTGCACG TGCTTCAGCA GCAGGGCCTG 
GTCCCAGAGC ACGTGGAGTC ACGGCCCCTC TACAGCCCCC TGCAGCCAGA CATTGAGCAG 
GGGAAGCTGC AGATGTGGGT CGACCTGTTT CCGAAGGCCC TGGGGCGCCC TGGACCTCCC 
TTCAACATCA CCCCACGGAG AGCCAGAAGG TTTTTCCTGC GTTCTATTAT CTGGAATACA 
AGAGATGTGA TCCTGGATGA CCTGAGCCTC ACGGGGGAGA AGATGAGCGA CATTTATGTG 
AAAGGTTGGA TGATTGGCTT TGAAGAACAC AAGCAAAAGA CAGACGTGCA TTATCGCTCC 
CTGGGAGGTG AAGGCAACTT CAACTGGAGG TTCATTTTCC CCTTCGACTA CCTGCCGGCT 
GAGCAAGTCT GTACCGTTGC CAAGAAGGAT GCCTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGAT ATTCCAGATC TGGGACAATG ACAAGTTCTC CTTTGATGAT 
TTTCTGGGCT CCCTGCAGCT CGATCTCAAC CGCATGCCCA AGCCAGCCAA GACAGCCGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCAG AATGGTTTGT GTCCCTTTTT 
GAGCAGAAAA CAGTGAAGGG CTGGTGGCCT TGTGTAGCAG AAGAGGGTGA GAAGAAGATA 
CTGGCGGGCA AGCTGGAAAT GACCTTGGAG ATTGTAACAG AGAGTGAGCA TGAGGAGCGG 
CCCGCTGGCC AGGGCAGGGA TGAGCCCAAC ATGAACCCTA AACTTGAGGA CCCAAGGCGC 
CCCGACACCT CCTTCCTGTG GTTCACCTCC CCATACAAGA CCATGAAGTT CATCCTGTGG 
CGGCGTTTCC GGTGGGCCAT CATCCTCTTT ATCATCCTCT TCATCCTGCT GCTGTTCCTG 
GCCATCTTCA TCTACGCCTT CCCGAACTAT GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832407.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688240.1 Macaca mulatta dysferlin (DYSF), transcript variant X20, mRNA.

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CloneID	OMb68151
Clone ID Related Accession (Same CDS sequence)	XM_028832407.1
Accession Version	XM_028832407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAACTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGGA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAGC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAACTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688240.1
CDS	137..6340
Translation	MLCCLLVRASNLPSAKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832407.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832407.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGGT GAGGGCCAGC AACCTCCCCA GTGCGAAGAA GGACCGGCGC 
AGCGACCCTG TCGCAAGCCT GACTTTCCGA GGGGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGTGTGA ACCCTGTATG GAATGAGGGC TTTGAATGGG ACCTCAAGGG CATCCCCTTG 
GACCAGGGCT CTGAGCTTCA TGTGGTGGTC AAAGACCATG AGACGATGGG GAGGAACAGG 
TTCCTGGGGG AAGCCAAGGT CCCACTCCGA GAGGTCCTCG CCACCCCTAG TCTGTCCGCC 
AGCTTCAATG CCCCCCTGCT GGACACCAAG AAGCAGCCCA CAGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACACCGCT GCCTGGAGCT GTGCCCCTGT TCCCGCCGCC TACTCCTCTG 
GAGCCCTCCC CAACTCTGCC TGACCTGGAT GTAGTGGCAG ACACAGGAGG AGAGGAAGAC 
ACAGAGGACC AGGGACTCAC TGGAGATGAG ACAGAGCCAT TCCTGGATCA AAGCGGAGGG 
CCGGGGGCTC CCACCACCCC AAGGAAACTA CCTTTGCATC CTCCACCCCA CTACCCCGGG 
ATCAAAAGAA AGCGAAGTGC ACCTACATCT AGAAAGCTGC TGTCAGACAA ACCGCAGGAC 
TTCCAGATTA GGGTCCAGGT GATCGAGGGG CGCCAGCTGC CCGGGGTGAA CATCAAGCCT 
GTGGTCAAGG TTACTGCTGC AGGGCAGACC AAGCGGACAC GGATCCACAA GGGAAACAGC 
CCACTCTTCA ATGAGACTCT TTTCTTCAAC TTGTTTGACT GTCCCGCGGA GCTGTTTGAT 
GAGCCCATCT TTATCACGGT GGTAGACTCC CGTTCTCTCA GGACAGATGC TCTCCTTGGG 
GAGTTCCGGA TGGACGTGGG CACCATTTAC AGAGAGCCCC GGCACGCCTA TCTCAGGAAG 
TGGCTGCTGC TCTCAGACCC TGATGACTTC TCTGCTGGGG CCAGAGGTTA CCTGAAAACA 
AGTCTTTGTG TGCTGGGGCC TGGGGATGAA GCGCCTCTGG AGAGAAAAGA GCCCTCTGAA 
GACAAGGAGG ACATTGAAAG CAACCTGCTC CGGCCCACAG GCGTGGCCCT GCGAGGAGCC 
CACTTCTGCC TGAAGGTCTT CCGGGCCGAG GACTTGCCAC AGATGGACGA TGCTGTGATG 
GACAACGTGA AACAGATCTT TGGCTTTGAG AGTAACAAGA AGAACTTGGT GGACCCCTTT 
GTGGAGGTCA GCTTTGCGGG GAAAATGCTC TGCAGCAAGA TCTTGGAGAA GACGGCCAAC 
CCTCAGTGGA ACCAGAACAT CACACTGCCT GCCATGTTTC CCTCCATGTG TGAAAAAATG 
AGGATTCGTG TCATAGACTG GGATCGCCTC ACTCACAATG ACATCGTGGC TACCGCCGAC 
CTGAGTATGT CGAAAATCTC TGCCCCTGGA GGAGACATAG AAGTGGATGA CCACCTGGGC 
TTCCTCCCCA CTTTTGGGCC CTGCTACATC AACCTCTATG GCAGTCCCAG AGAGTTCACG 
GGCTTCCTGG ACCCCTACGC AGAGCTCAAC ACAGGCAAGG GGGAAGGTGT GGCTTATCGT 
GGCCGGCTTC TGCTTTCCCT GGAGACCAAG CTGGTGGAGC ACAGTGAGCA GAAGGTGGAG 
GACCTTCCTG CGGATGACAT CCTCCGGGTG GAGAAGTACC TTAGGAGGCG CAAGTACTCC 
CTGTTTGCGG CCTTCTACTC GGCCACCATG CTGCAGGATG TGGATGACGC CATCCAGTTT 
GAGGTCAGCA TCGGGAACTA TGGGAACAAG TTCGACATGA CCTGCCTGCC GCTGGCCTCC 
ACCACTCAGT ATAGCCGTGC AGTCTTTGAC GGGTGCCACT ACTACTACCT ACCCTGGGGC 
AACGTGAAAC CTGTGGTGGT GCTGTCATCC TACTGGGAGG ACATCAGCCA TAGAATCGAG 
ACTCAGAACC AGCTGCTTGG GATTGCTGAC CGGCTGGAAG CTGGCCTGGA GCAGGTCCAC 
CTGGCCCTGA AGGCGCAGTG CTCCACGGAG GACGTGGACT CACTGGTGGC TCAGATGATG 
GATGAGCTCA TCGCAGGCTG CAGCCAGCCT CTGGGTGATA TCCATGAGAT ACCCTCTGCC 
ACCCACCTGG ACGAGTACCT GTACCGGCTG CGCACCCGTC ACCTGAGCCA AATCACGGAG 
GCTGCCCTGG CCCTGAAGCT TGGCCACAGT GAGCTCCCTG CTGCTCTGGA TCAGGCTGAG 
GACTGGCTCC TGCGTCTGCG TGCCCTCGCT GAGGAGCCCC AGAACAGCCT GCCGGATATC 
ATCATCTGGA TGCTGCAGGG AGACAAGCGT GTGGCATACC AGCGGGTGCC CGCCCACCAA 
GTCCTCTTCT CCCGGCGGGG TGCCAACTTC TGTGGCAAGA ATTGTGGGAA GTTGCAGACA 
ATCTTTCTGA AATATCCGAT GGAGAAGGTG CCTGGGGCCC GGATGCCAGT GCAGATACGG 
GTCAAGCTGT GGTTTGGGCT CTCTGTGGAT GAGAAGGAGT TCAACCAGTT TGCTGAGGGG 
AAGCTGTCTG TCTTTGCTGA AACCTATGAG AACCAGACCA AGTTGGCCCT GGTCGGGAAC 
TGGGGCACAA CGGGCCTCAC CTACCCCAAG TTTTCTGACG TCACCGGCAA GATCAAGCTA 
CCCAAAGAGA GCTTCCGCCC CTCGGCCGGC TGGACCTGGG CTGGAGATTG GTTCGTGTGT 
CCGGAGAAGA CCCTGCTCCA TGACATGGAC GCCGGTCACC TGAACTTTGT GGAGGAGGTG 
TTTGAGAACC AGACCCGGCT TCCCGGAGGC CAGTGGATCT ACATGAGTGA CAACTACACC 
GATGTGAACG GGGAGAAGGT GCTTCCCAAA GATGACATTG AGTGCCCACT GGGCTGGAAG 
TGGGAAGATG AGGAATGGTC CACAGACCTC AACCGGGCTG TCGATGAGCA AGGCTGGGAG 
TATAGCATCA CCATCCCCCC GGACCGGAAG CCGAAGCACT GGGTCCCTGC TGAGAAGATG 
TACTACACAC ACCGACGGCG GCGCTGGGTG CGCCTGCGGA GGAGGGATCT CAGCCAGATG 
GAAGCGCTGA AGAGGCATAG GCAGGCGGAG GCAGAGGGCG AGGGCTGGGA GTACGCCTCT 
CTTTTTGGCT GGAAGTTCCA CCTCGAGTAC CGCAAGACAG ACGCCTTCCG CCGCCGCCGC 
TGGCGCCGCC GCATGGAGCC CCTGGAGAAG ACGGGGCCTG CAGCTGTGTT TGCCCTCGAG 
GGGGCCCTGG GCGGCGTGGT GGACGACAAG AGTGAAGATT CCATGTCCGT CTCCACCTTG 
AGCTTTGGTG TGAACAGACC CACAATTTCC TGCATATTTG ACTATGGGAA CCGCTACCAT 
CTACGCTGCT ACATGTACCA GGCCCGGGAC CTGGCTGCGA TGGACAAGGA CTCTTTTTCT 
GATCCCTATG CCATCGTCTC CTTCCTGCAC CAGAGCCAGA AGACGGTGGT GGTGAAGAAC 
ACCCTTAACC CCACCTGGGA CCAGACGCTC ATCTTCTACG AGATCGAGAT CTTCGGCGAG 
CCGGCCACAG TTGCTGAGCA GCCGCCCAGC ATTGTGGTGG AGCTGTACGA CCATGACACT 
TACGGCGCAG ACGAGTTTAT GGGCCGCTGC ATCTGTCAGC CGAGTTTGGA ACGGACGCCC 
CGACTGGCCT GGTTCCCACT GACCAGGGGC AGCCAGCCGA CGGGGGAGCT GCTGGCCTCT 
TTTGAGCTCA TCCAGAGAGA GAAGCCGGCC ATCCACCATA TTCCTGGTTT TGAGGTGCAG 
GAGACATCGA GGATCCTGGA TGAGTCTGAG GACACAGACC TGCCCTACCC ACCACCCCAG 
AGGGAGGCCA ACATCTACAT GGTTCCTCAG AACATCAAGC CAGCGCTCCA GCGTACCGCC 
ATCGAGGTCC TGGCATGGGG CCTGCGGAAC ATGAAGAGTT ACCAGCTGGC CAGCATCTCC 
TCCCCGAGCC TTGTGATAGA GTGTGGGGGC CAGACGGTGC AGTCCTGTGT CATCAGGAAC 
CTCCGGAAGA ACCCCAACTT TGACATCTGT ACCCTCTTCA TGGAAGTGAT GCTGCCCAGG 
GAGGAGCTCT ACTGCCCCCC CATCACCATC AAGGTCATCG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGC TCCCTGGAGA GCTTCCTGTG TGACCCCTAC 
TCGGCGGAGA GTCCATCCCC ACAGGGTGGC CCAGACGATG TGAGCCTACT CAGTCCTGGG 
GAAGACGTGC TCATCGACAT TGATGACAAG GAGCCCCTCA TCCCCATCCA GGAGGAAGAG 
TTTATCGATT GGTGGAGCAA ATTCTTTGCC TCCATAGGGG AGAGGGAAAA GTGCGGCTCC 
TACTTGGAGA AGGATTTTGA CACCCTGAAG GTCTATGACA CACAGCTGGA GAATGTGGAG 
GCCTTTGAGG GCCTGTCTGA CTTTTGTAAC ACCTTCAAGC TGTACCGGGG CAAGACGCAG 
GAGGAGACAG AAGATCCATC TGTCATTGGC GAATTTAAGG GCCTCTTCAA AATTTATCCC 
CTCCCAGAAG ACCCAGCCAT CCCCATGCCC CCAAGACAGT TCCACCAGCT GGCCGCGCAG 
GGACCCCAGG AGTGCTTGGT CCGTATCTAC ATTGTCCGAG CATTTGGCCT GCAGCCCAAG 
GACCCCAATG GGAAGTGTGA TCCTTACATC AAGATCTCCA TAGGGAAGAA ATCAGTGAGT 
GACCAGGATA ACTACATCCC CTGCACGTTG GAGCCTGTGT TTGGAAAGAT GTTTGAGCTG 
ACCTGCACTC TGCCTCTGGA GAAGGACCTG AAGGTCACTC TCTACGACTA TGACCTCCTC 
TCCAAGGATG AAAAGATTGG TGAGACGGTC ATCGACCTGG AGAACAGGCT GCTGTCCAAG 
TTTGCGGCTC GCTGTGGACT CCCGCAGACC TACTGTGTCT CTGGACCGAA CCAGTGGCGG 
GACCAGCTCC GCCCCTCCCA GCTCCTCCAC CTCTTCTGCC AGCAGCGCAG AGTCAAGGCG 
CCTGTGTACC GGACAGACCG TGTGATGTTT CAGGATAAAG AATACTCCAT TGAAGAAATA 
GAGGCTGGCA GGATCCCAAA CCCACACCTG GGCCCCGTGG AGGAGCGTCT GGCTCTGCAC 
GTGCTTCAGC AGCAGGGCCT GGTCCCAGAG CACGTGGAGT CACGGCCCCT CTACAGCCCC 
CTGCAGCCAG ACATTGAGCA GGGGAAGCTG CAGATGTGGG TCGACCTGTT TCCGAAGGCC 
CTGGGGCGCC CTGGACCTCC CTTCAACATC ACCCCACGGA GAGCCAGAAG GTTTTTCCTG 
CGTTCTATTA TCTGGAATAC AAGAGATGTG ATCCTGGATG ACCTGAGCCT CACGGGGGAG 
AAGATGAGCG ACATTTATGT GAAAGGTTGG ATGATTGGCT TTGAAGAACA CAAGCAAAAG 
ACAGACGTGC ATTATCGCTC CCTGGGAGGT GAAGGCAACT TCAACTGGAG GTTCATTTTC 
CCCTTCGACT ACCTGCCGGC TGAGCAAGTC TGTACCGTTG CCAAGAAGGA TGCCTTCTGG 
AGGCTGGACA AGACTGAGAG CAAAATCCCA GCACGAGTGA TATTCCAGAT CTGGGACAAT 
GACAAGTTCT CCTTTGATGA TTTTCTGGGC TCCCTGCAGC TCGATCTCAA CCGCATGCCC 
AAGCCAGCCA AGACAGCCGA GAAGTGCTCC TTGGACCAGC TGGATGACAC TTTCCACCCA 
GAATGGTTTG TGTCCCTTTT TGAGCAGAAA ACAGTGAAGG GCTGGTGGCC TTGTGTAGCA 
GAAGAGGGTG AGAAGAAGAT ACTGGCGGGC AAGCTGGAAA TGACCTTGGA GATTGTAACA 
GAGAGTGAGC ATGAGGAGCG GCCCGCTGGC CAGGGCAGGG ATGAGCCCAA CATGAACCCT 
AAACTTGAGG ACCCAAGGCG CCCCGACACC TCCTTCCTGT GGTTCACCTC CCCATACAAG 
ACCATGAAGT TCATCCTGTG GCGGCGTTTC CGGTGGGCCA TCATCCTCTT TATCATCCTC 
TTCATCCTGC TGCTGTTCCT GGCCATCTTC ATCTACGCCT TCCCGAACTA TGCTGCCATG 
AAGCTGGTGA AGCCCTTCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832409.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688242.1 Macaca mulatta dysferlin (DYSF), transcript variant X21, mRNA.

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CloneID	OMb68152
Clone ID Related Accession (Same CDS sequence)	XM_028832409.1
Accession Version	XM_028832409.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA ACTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGGAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAGCCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAA 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688242.1
CDS	376..6576
Translation	MLRVFILYAENVHTPDTDISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVADTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEVDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832409.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832409.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTATGCTGAG AACGTCCACA CACCCGACAC CGACATCAGC 
GATGCTTACT GCTCTGCTGT GTTTGCAGGG GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGTGTGAACC CTGTATGGAA TGAGGGCTTT GAATGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGGGCTCTG AGCTTCATGT GGTGGTCAAA GACCATGAGA CGATGGGGAG GAACAGGTTC 
CTGGGGGAAG CCAAGGTCCC ACTCCGAGAG GTCCTCGCCA CCCCTAGTCT GTCCGCCAGC 
TTCAATGCCC CCCTGCTGGA CACCAAGAAG CAGCCCACAG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CACCGCTGCC TGGAGCTGTG CCCCTGTTCC CGCCGCCTAC TCCTCTGGAG 
CCCTCCCCAA CTCTGCCTGA CCTGGATGTA GTGGCAGACA CAGGAGGAGA GGAAGACACA 
GAGGACCAGG GACTCACTGG AGATGAGACA GAGCCATTCC TGGATCAAAG CGGAGGGCCG 
GGGGCTCCCA CCACCCCAAG GAAACTACCT TTGCATCCTC CACCCCACTA CCCCGGGATC 
AAAAGAAAGC GAAGTGCACC TACATCTAGA AAGCTGCTGT CAGACAAACC GCAGGACTTC 
CAGATTAGGG TCCAGGTGAT CGAGGGGCGC CAGCTGCCCG GGGTGAACAT CAAGCCTGTG 
GTCAAGGTTA CTGCTGCAGG GCAGACCAAG CGGACACGGA TCCACAAGGG AAACAGCCCA 
CTCTTCAATG AGACTCTTTT CTTCAACTTG TTTGACTGTC CCGCGGAGCT GTTTGATGAG 
CCCATCTTTA TCACGGTGGT AGACTCCCGT TCTCTCAGGA CAGATGCTCT CCTTGGGGAG 
TTCCGGATGG ACGTGGGCAC CATTTACAGA GAGCCCCGGC ACGCCTATCT CAGGAAGTGG 
CTGCTGCTCT CAGACCCTGA TGACTTCTCT GCTGGGGCCA GAGGTTACCT GAAAACAAGT 
CTTTGTGTGC TGGGGCCTGG GGATGAAGCG CCTCTGGAGA GAAAAGAGCC CTCTGAAGAC 
AAGGAGGACA TTGAAAGCAA CCTGCTCCGG CCCACAGGCG TGGCCCTGCG AGGAGCCCAC 
TTCTGCCTGA AGGTCTTCCG GGCCGAGGAC TTGCCACAGA TGGACGATGC TGTGATGGAC 
AACGTGAAAC AGATCTTTGG CTTTGAGAGT AACAAGAAGA ACTTGGTGGA CCCCTTTGTG 
GAGGTCAGCT TTGCGGGGAA AATGCTCTGC AGCAAGATCT TGGAGAAGAC GGCCAACCCT 
CAGTGGAACC AGAACATCAC ACTGCCTGCC ATGTTTCCCT CCATGTGTGA AAAAATGAGG 
ATTCGTGTCA TAGACTGGGA TCGCCTCACT CACAATGACA TCGTGGCTAC CGCCGACCTG 
AGTATGTCGA AAATCTCTGC CCCTGGAGGA GACATAGAAG TGGATGACCA CCTGGGCTTC 
CTCCCCACTT TTGGGCCCTG CTACATCAAC CTCTATGGCA GTCCCAGAGA GTTCACGGGC 
TTCCTGGACC CCTACGCAGA GCTCAACACA GGCAAGGGGG AAGGTGTGGC TTATCGTGGC 
CGGCTTCTGC TTTCCCTGGA GACCAAGCTG GTGGAGCACA GTGAGCAGAA GGTGGAGGAC 
CTTCCTGCGG ATGACATCCT CCGGGTGGAG AAGTACCTTA GGAGGCGCAA GTACTCCCTG 
TTTGCGGCCT TCTACTCGGC CACCATGCTG CAGGATGTGG ATGACGCCAT CCAGTTTGAG 
GTCAGCATCG GGAACTATGG GAACAAGTTC GACATGACCT GCCTGCCGCT GGCCTCCACC 
ACTCAGTATA GCCGTGCAGT CTTTGACGGG TGCCACTACT ACTACCTACC CTGGGGCAAC 
GTGAAACCTG TGGTGGTGCT GTCATCCTAC TGGGAGGACA TCAGCCATAG AATCGAGACT 
CAGAACCAGC TGCTTGGGAT TGCTGACCGG CTGGAAGCTG GCCTGGAGCA GGTCCACCTG 
GCCCTGAAGG CGCAGTGCTC CACGGAGGAC GTGGACTCAC TGGTGGCTCA GATGATGGAT 
GAGCTCATCG CAGGCTGCAG CCAGCCTCTG GGTGATATCC ATGAGATACC CTCTGCCACC 
CACCTGGACG AGTACCTGTA CCGGCTGCGC ACCCGTCACC TGAGCCAAAT CACGGAGGCT 
GCCCTGGCCC TGAAGCTTGG CCACAGTGAG CTCCCTGCTG CTCTGGATCA GGCTGAGGAC 
TGGCTCCTGC GTCTGCGTGC CCTCGCTGAG GAGCCCCAGA ACAGCCTGCC GGATATCATC 
ATCTGGATGC TGCAGGGAGA CAAGCGTGTG GCATACCAGC GGGTGCCCGC CCACCAAGTC 
CTCTTCTCCC GGCGGGGTGC CAACTTCTGT GGCAAGAATT GTGGGAAGTT GCAGACAATC 
TTTCTGAAAT ATCCGATGGA GAAGGTGCCT GGGGCCCGGA TGCCAGTGCA GATACGGGTC 
AAGCTGTGGT TTGGGCTCTC TGTGGATGAG AAGGAGTTCA ACCAGTTTGC TGAGGGGAAG 
CTGTCTGTCT TTGCTGAAAC CTATGAGAAC CAGACCAAGT TGGCCCTGGT CGGGAACTGG 
GGCACAACGG GCCTCACCTA CCCCAAGTTT TCTGACGTCA CCGGCAAGAT CAAGCTACCC 
AAAGAGAGCT TCCGCCCCTC GGCCGGCTGG ACCTGGGCTG GAGATTGGTT CGTGTGTCCG 
GAGAAGACCC TGCTCCATGA CATGGACGCC GGTCACCTGA ACTTTGTGGA GGAGGTGTTT 
GAGAACCAGA CCCGGCTTCC CGGAGGCCAG TGGATCTACA TGAGTGACAA CTACACCGAT 
GTGAACGGGG AGAAGGTGCT TCCCAAAGAT GACATTGAGT GCCCACTGGG CTGGAAGTGG 
GAAGATGAGG AATGGTCCAC AGACCTCAAC CGGGCTGTCG ATGAGCAAGG CTGGGAGTAT 
AGCATCACCA TCCCCCCGGA CCGGAAGCCG AAGCACTGGG TCCCTGCTGA GAAGATGTAC 
TACACACACC GACGGCGGCG CTGGGTGCGC CTGCGGAGGA GGGATCTCAG CCAGATGGAA 
GCGCTGAAGA GGCATAGGCA GGCGGAGGCA GAGGGCGAGG GCTGGGAGTA CGCCTCTCTT 
TTTGGCTGGA AGTTCCACCT CGAGTACCGC AAGACAGACG CCTTCCGCCG CCGCCGCTGG 
CGCCGCCGCA TGGAGCCCCT GGAGAAGACG GGGCCTGCAG CTGTGTTTGC CCTCGAGGGG 
GCCCTGGGCG GCGTGGTGGA CGACAAGAGT GAAGATTCCA TGTCCGTCTC CACCTTGAGC 
TTTGGTGTGA ACAGACCCAC AATTTCCTGC ATATTTGACT ATGGGAACCG CTACCATCTA 
CGCTGCTACA TGTACCAGGC CCGGGACCTG GCTGCGATGG ACAAGGACTC TTTTTCTGAT 
CCCTATGCCA TCGTCTCCTT CCTGCACCAG AGCCAGAAGA CGGTGGTGGT GAAGAACACC 
CTTAACCCCA CCTGGGACCA GACGCTCATC TTCTACGAGA TCGAGATCTT CGGCGAGCCG 
GCCACAGTTG CTGAGCAGCC GCCCAGCATT GTGGTGGAGC TGTACGACCA TGACACTTAC 
GGCGCAGACG AGTTTATGGG CCGCTGCATC TGTCAGCCGA GTTTGGAACG GACGCCCCGA 
CTGGCCTGGT TCCCACTGAC CAGGGGCAGC CAGCCGACGG GGGAGCTGCT GGCCTCTTTT 
GAGCTCATCC AGAGAGAGAA GCCGGCCATC CACCATATTC CTGGTTTTGA GGTGCAGGAG 
ACATCGAGGA TCCTGGATGA GTCTGAGGAC ACAGACCTGC CCTACCCACC ACCCCAGAGG 
GAGGCCAACA TCTACATGGT TCCTCAGAAC ATCAAGCCAG CGCTCCAGCG TACCGCCATC 
GAGGTCCTGG CATGGGGCCT GCGGAACATG AAGAGTTACC AGCTGGCCAG CATCTCCTCC 
CCGAGCCTTG TGATAGAGTG TGGGGGCCAG ACGGTGCAGT CCTGTGTCAT CAGGAACCTC 
CGGAAGAACC CCAACTTTGA CATCTGTACC CTCTTCATGG AAGTGATGCT GCCCAGGGAG 
GAGCTCTACT GCCCCCCCAT CACCATCAAG GTCATCGATA ACCGCCAGTT TGGCCGCCGG 
CCTGTGGTGG GCCAGTGTAC CATCCGCTCC CTGGAGAGCT TCCTGTGTGA CCCCTACTCG 
GCGGAGAGTC CATCCCCACA GGGTGGCCCA GACGATGTGA GCCTACTCAG TCCTGGGGAA 
GACGTGCTCA TCGACATTGA TGACAAGGAG CCCCTCATCC CCATCCAGGA GGAAGAGTTT 
ATCGATTGGT GGAGCAAATT CTTTGCCTCC ATAGGGGAGA GGGAAAAGTG CGGCTCCTAC 
TTGGAGAAGG ATTTTGACAC CCTGAAGGTC TATGACACAC AGCTGGAGAA TGTGGAGGCC 
TTTGAGGGCC TGTCTGACTT TTGTAACACC TTCAAGCTGT ACCGGGGCAA GACGCAGGAG 
GAGACAGAAG ATCCATCTGT CATTGGCGAA TTTAAGGGCC TCTTCAAAAT TTATCCCCTC 
CCAGAAGACC CAGCCATCCC CATGCCCCCA AGACAGTTCC ACCAGCTGGC CGCGCAGGGA 
CCCCAGGAGT GCTTGGTCCG TATCTACATT GTCCGAGCAT TTGGCCTGCA GCCCAAGGAC 
CCCAATGGGA AGTGTGATCC TTACATCAAG ATCTCCATAG GGAAGAAATC AGTGAGTGAC 
CAGGATAACT ACATCCCCTG CACGTTGGAG CCTGTGTTTG GAAAGATGTT TGAGCTGACC 
TGCACTCTGC CTCTGGAGAA GGACCTGAAG GTCACTCTCT ACGACTATGA CCTCCTCTCC 
AAGGATGAAA AGATTGGTGA GACGGTCATC GACCTGGAGA ACAGGCTGCT GTCCAAGTTT 
GCGGCTCGCT GTGGACTCCC GCAGACCTAC TGTGTCTCTG GACCGAACCA GTGGCGGGAC 
CAGCTCCGCC CCTCCCAGCT CCTCCACCTC TTCTGCCAGC AGCGCAGAGT CAAGGCGCCT 
GTGTACCGGA CAGACCGTGT GATGTTTCAG GATAAAGAAT ACTCCATTGA AGAAATAGAG 
GCTGGCAGGA TCCCAAACCC ACACCTGGGC CCCGTGGAGG AGCGTCTGGC TCTGCACGTG 
CTTCAGCAGC AGGGCCTGGT CCCAGAGCAC GTGGAGTCAC GGCCCCTCTA CAGCCCCCTG 
CAGCCAGACA TTGAGCAGGG GAAGCTGCAG ATGTGGGTCG ACCTGTTTCC GAAGGCCCTG 
GGGCGCCCTG GACCTCCCTT CAACATCACC CCACGGAGAG CCAGAAGGTT TTTCCTGCGT 
TCTATTATCT GGAATACAAG AGATGTGATC CTGGATGACC TGAGCCTCAC GGGGGAGAAG 
ATGAGCGACA TTTATGTGAA AGGTTGGATG ATTGGCTTTG AAGAACACAA GCAAAAGACA 
GACGTGCATT ATCGCTCCCT GGGAGGTGAA GGCAACTTCA ACTGGAGGTT CATTTTCCCC 
TTCGACTACC TGCCGGCTGA GCAAGTCTGT ACCGTTGCCA AGAAGGATGC CTTCTGGAGG 
CTGGACAAGA CTGAGAGCAA AATCCCAGCA CGAGTGATAT TCCAGATCTG GGACAATGAC 
AAGTTCTCCT TTGATGATTT TCTGGGCTCC CTGCAGCTCG ATCTCAACCG CATGCCCAAG 
CCAGCCAAGA CAGCCGAGAA GTGCTCCTTG GACCAGCTGG ATGACACTTT CCACCCAGAA 
TGGTTTGTGT CCCTTTTTGA GCAGAAAACA GTGAAGGGCT GGTGGCCTTG TGTAGCAGAA 
GAGGGTGAGA AGAAGATACT GGCGGGCAAG CTGGAAATGA CCTTGGAGAT TGTAACAGAG 
AGTGAGCATG AGGAGCGGCC CGCTGGCCAG GGCAGGGATG AGCCCAACAT GAACCCTAAA 
CTTGAGGACC CAAGGCGCCC CGACACCTCC TTCCTGTGGT TCACCTCCCC ATACAAGACC 
ATGAAGTTCA TCCTGTGGCG GCGTTTCCGG TGGGCCATCA TCCTCTTTAT CATCCTCTTC 
ATCCTGCTGC TGTTCCTGGC CATCTTCATC TACGCCTTCC CGAACTATGC TGCCATGAAG 
CTGGTGAAGC CCTTCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_028832410.1 ) cDNA ORF clone, Macaca mulatta(Rhesus monkey) -> XP_028688243.1 Macaca mulatta dysferlin (DYSF), transcript variant X22, mRNA.

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CloneID	OMb68153
Clone ID Related Accession (Same CDS sequence)	XM_028832410.1
Accession Version	XM_028832410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-25
Organism	Macaca mulatta(Rhesus monkey)
Product	dysferlin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041766.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Macaca mulatta Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCCAG TTCTTCAGGA GGCCTCGCTG GTCCTGCAGG TGTCCTACAC ACCGCTGCCT 
GGAGCTGTGC CCCTGTTCCC GCCGCCTACT CCTCTGGAGC CCTCCCCAAC TCTGCCTGAC 
CTGGATGTAG TGGCAGGCGG GGGACAGAGC CGGGCCGAGA CTTGGTCCCT GCTCAGTGAC 
AGCACCGTGG ACACGAGATA CTCTGGAAAG AAGTGGCCGG CCCCCATGGA CACAGGAGGA 
GAGGAAGACA CAGAGGACCA GGGACTCACT GGAGATGAGA CAGAGCCATT CCTGGATCAA 
AGCGGAGGGC CGGGGGCTCC CACCACCCCA AGGAAACTAC CTTTGCATCC TCCACCCCAC 
TACCCCGGGA TCAAAAGAAA GCGAAGTGCA CCTACATCTA GAAAGCTGCT GTCAGACAAA 
CCGCAGGACT TCCAGATTAG GGTCCAGGTG ATCGAGGGGC GCCAGCTGCC CGGGGTGAAC 
ATCAAGCCTG TGGTCAAGGT TACTGCTGCA GGGCAGACCA AGCGGACACG GATCCACAAG 
GGAAACAGCC CACTCTTCAA TGAGACTCTT TTCTTCAACT TGTTTGACTG TCCCGCGGAG 
CTGTTTGATG AGCCCATCTT TATCACGGTG GTAGACTCCC GTTCTCTCAG GACAGATGCT 
CTCCTTGGGG AGTTCCGGAT GGACGTGGGC ACCATTTACA GAGAGCCCCG GCACGCCTAT 
CTCAGGAAGT GGCTGCTGCT CTCAGACCCT GATGACTTCT CTGCTGGGGC CAGAGGTTAC 
CTGAAAACAA GTCTTTGTGT GCTGGGGCCT GGGGATGAAG CGCCTCTGGA GAGAAAAGAG 
CCCTCTGAAG ACAAGGAGGA CATTGAAAGC AACCTGCTCC GGCCCACAGG CGTGGCCCTG 
CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC CGGGCCGAGG ACTTGCCACA GATGGACGAT 
GCTGTGATGG ACAACGTGAA ACAGATCTTT GGCTTTGAGA GTAACAAGAA GAACTTGGTG 
GACCCCTTTG TGGAGGTCAG CTTTGCGGGG AAAATGCTCT GCAGCAAGAT CTTGGAGAAG 
ACGGCCAACC CTCAGTGGAA CCAGAACATC ACACTGCCTG CCATGTTTCC CTCCATGTGT 
GAAAAAATGA GGATTCGTGT CATAGACTGG GATCGCCTCA CTCACAATGA CATCGTGGCT 
ACCGCCGACC TGAGTATGTC GAAAATCTCT GCCCCTGGAG GAGACATAGA AGAGGAGACT 
GCAGGTGCCG TCAAGCCTTC GAAAGTCTCA GACCTGGATG ACCACCTGGG CTTCCTCCCC 
ACTTTTGGGC CCTGCTACAT CAACCTCTAT GGCAGTCCCA GAGAGTTCAC GGGCTTCCTG 
GACCCCTACG CAGAGCTCAA CACAGGCAAG GGGGAAGGTG TGGCTTATCG TGGCCGGCTT 
CTGCTTTCCC TGGAGACCAA GCTGGTGGAG CACAGTGAGC AGAAGGTGGA GGACCTTCCT 
GCGGATGACA TCCTCCGGGT GGAGAAGTAC CTTAGGAGGC GCAAGTACTC CCTGTTTGCG 
GCCTTCTACT CGGCCACCAT GCTGCAGGAT GTGGATGACG CCATCCAGTT TGAGGTCAGC 
ATCGGGAACT ATGGGAACAA GTTCGACATG ACCTGCCTGC CGCTGGCCTC CACCACTCAG 
TATAGCCGTG CAGTCTTTGA CGGGTGCCAC TACTACTACC TACCCTGGGG CAACGTGAAA 
CCTGTGGTGG TGCTGTCATC CTACTGGGAG GACATCAGCC ATAGAATCGA GACTCAGAAC 
CAGCTGCTTG GGATTGCTGA CCGGCTGGAA GCTGGCCTGG AGCAGGTCCA CCTGGCCCTG 
AAGGCGCAGT GCTCCACGGA GGACGTGGAC TCACTGGTGG CTCAGATGAT GGATGAGCTC 
ATCGCAGGCT GCAGCCAGCC TCTGGGTGAT ATCCATGAGA TACCCTCTGC CACCCACCTG 
GACGAGTACC TGTACCGGCT GCGCACCCGT CACCTGAGCC AAATCACGGA GGCTGCCCTG 
GCCCTGAAGC TTGGCCACAG TGAGCTCCCT GCTGCTCTGG ATCAGGCTGA GGACTGGCTC 
CTGCGTCTGC GTGCCCTCGC TGAGGAGCCC CAGAACAGCC TGCCGGATAT CATCATCTGG 
ATGCTGCAGG GAGACAAGCG TGTGGCATAC CAGCGGGTGC CCGCCCACCA AGTCCTCTTC 
TCCCGGCGGG GTGCCAACTT CTGTGGCAAG AATTGTGGGA AGTTGCAGAC AATCTTTCTG 
AAATATCCGA TGGAGAAGGT GCCTGGGGCC CGGATGCCAG TGCAGATACG GGTCAAGCTG 
TGGTTTGGGC TCTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG GAAGCTGTCT 
GTCTTTGCTG AAACCTATGA GAACCAGACC AAGTTGGCCC TGGTCGGGAA CTGGGGCACA 
ACGGGCCTCA CCTACCCCAA GTTTTCTGAC GTCACCGGCA AGATCAAGCT ACCCAAAGAG 
AGCTTCCGCC CCTCGGCCGG CTGGACCTGG GCTGGAGATT GGTTCGTGTG TCCGGAGAAG 
ACCCTGCTCC ATGACATGGA CGCCGGTCAC CTGAACTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TTCCCGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC CGATGTGAAC 
GGGGAGAAGG TGCTTCCCAA AGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAGGAATGGT CCACAGACCT CAACCGGGCT GTCGATGAGC AAGGCTGGGA GTATAGCATC 
ACCATCCCCC CGGACCGGAA GCCGAAGCAC TGGGTCCCTG CTGAGAAGAT GTACTACACA 
CACCGACGGC GGCGCTGGGT GCGCCTGCGG AGGAGGGATC TCAGCCAGAT GGAAGCGCTG 
AAGAGGCATA GGCAGGCGGA GGCAGAGGGC GAGGGCTGGG AGTACGCCTC TCTTTTTGGC 
TGGAAGTTCC ACCTCGAGTA CCGCAAGACA GACGCCTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAGC CCCTGGAGAA GACGGGGCCT GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG 
GGCGGCGTGG TGGACGACAA GAGTGAAGAT TCCATGTCCG TCTCCACCTT GAGCTTTGGT 
GTGAACAGAC CCACAATTTC CTGCATATTT GACTATGGGA ACCGCTACCA TCTACGCTGC 
TACATGTACC AGGCCCGGGA CCTGGCTGCG ATGGACAAGG ACTCTTTTTC TGATCCCTAT 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACGGTGG TGGTGAAGAA CACCCTTAAC 
CCCACCTGGG ACCAGACGCT CATCTTCTAC GAGATCGAGA TCTTCGGCGA GCCGGCCACA 
GTTGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTACG ACCATGACAC TTACGGCGCA 
GACGAGTTTA TGGGCCGCTG CATCTGTCAG CCGAGTTTGG AACGGACGCC CCGACTGGCC 
TGGTTCCCAC TGACCAGGGG CAGCCAGCCG ACGGGGGAGC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AGAAGCCGGC CATCCACCAT ATTCCTGGTT TTGAGGTGCA GGAGACATCG 
AGGATCCTGG ATGAGCTGTG TGCTCCTGCC TTCTTCCTCC AGGGCCTTCT CCAAACGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT CTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCTTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCAGGGATGA GCCCAACATG AACCCTAAAC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTTATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028688243.1
CDS	3605..9724
Translation	MVPVLQEASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDVVAGGGQSRAETWSLLSDSTVDTRYSGKKWPAPMDTGGEEDTEDQGLTGDETEPFLDQSGGPGAPTTPRKLPLHPPPHYPGIKRKRSAPTSRKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVVKVTAAGQTKRTRIHKGNSPLFNETLFFNLFDCPAELFDEPIFITVVDSRSLRTDALLGEFRMDVGTIYREPRHAYLRKWLLLSDPDDFSAGARGYLKTSLCVLGPGDEAPLERKEPSEDKEDIESNLLRPTGVALRGAHFCLKVFRAEDLPQMDDAVMDNVKQIFGFESNKKNLVDPFVEVSFAGKMLCSKILEKTANPQWNQNITLPAMFPSMCEKMRIRVIDWDRLTHNDIVATADLSMSKISAPGGDIEEETAGAVKPSKVSDLDDHLGFLPTFGPCYINLYGSPREFTGFLDPYAELNTGKGEGVAYRGRLLLSLETKLVEHSEQKVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDMTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRIETQNQLLGIADRLEAGLEQVHLALKAQCSTEDVDSLVAQMMDELIAGCSQPLGDIHEIPSATHLDEYLYRLRTRHLSQITEAALALKLGHSELPAALDQAEDWLLRLRALAEEPQNSLPDIIIWMLQGDKRVAYQRVPAHQVLFSRRGANFCGKNCGKLQTIFLKYPMEKVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVTGKIKLPKESFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLNFVEEVFENQTRLPGGQWIYMSDNYTDVNGEKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDKSEDSMSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQTLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLTRGSQPTGELLASFELIQREKPAIHHIPGFEVQETSRILDELCAPAFFLQGLLQTSEDTDLPYPPPQREANIYMVPQNIKPALQRTAIEVLAWGLRNMKSYQLASISSPSLVIECGGQTVQSCVIRNLRKNPNFDICTLFMEVMLPREELYCPPITIKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAESPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQLADGLSSLAPTNTASPPSSPHEEEFIDWWSKFFASIGEREKCGSYLEKDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKVTLYDYDLLSKDEKIGETVIDLENRLLSKFAARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRVKAPVYRTDRVMFQDKEYSIEEIEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFPKALGRPGPPFNITPRRARRFFLRSIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVAKKDAFWRLDKTESKIPARVIFQIWDNDKFSFDDFLGSLQLDLNRMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVTESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_028832410.1
Organism	Macaca mulatta(Rhesus monkey)
Definition	Macaca mulatta dysferlin (DYSF), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_028832410.1

ORF Insert Sequence:

ATGGTGCCAG TTCTTCAGGA GGCCTCGCTG GTCCTGCAGG TGTCCTACAC ACCGCTGCCT 
GGAGCTGTGC CCCTGTTCCC GCCGCCTACT CCTCTGGAGC CCTCCCCAAC TCTGCCTGAC 
CTGGATGTAG TGGCAGGCGG GGGACAGAGC CGGGCCGAGA CTTGGTCCCT GCTCAGTGAC 
AGCACCGTGG ACACGAGATA CTCTGGAAAG AAGTGGCCGG CCCCCATGGA CACAGGAGGA 
GAGGAAGACA CAGAGGACCA GGGACTCACT GGAGATGAGA CAGAGCCATT CCTGGATCAA 
AGCGGAGGGC CGGGGGCTCC CACCACCCCA AGGAAACTAC CTTTGCATCC TCCACCCCAC 
TACCCCGGGA TCAAAAGAAA GCGAAGTGCA CCTACATCTA GAAAGCTGCT GTCAGACAAA 
CCGCAGGACT TCCAGATTAG GGTCCAGGTG ATCGAGGGGC GCCAGCTGCC CGGGGTGAAC 
ATCAAGCCTG TGGTCAAGGT TACTGCTGCA GGGCAGACCA AGCGGACACG GATCCACAAG 
GGAAACAGCC CACTCTTCAA TGAGACTCTT TTCTTCAACT TGTTTGACTG TCCCGCGGAG 
CTGTTTGATG AGCCCATCTT TATCACGGTG GTAGACTCCC GTTCTCTCAG GACAGATGCT 
CTCCTTGGGG AGTTCCGGAT GGACGTGGGC ACCATTTACA GAGAGCCCCG GCACGCCTAT 
CTCAGGAAGT GGCTGCTGCT CTCAGACCCT GATGACTTCT CTGCTGGGGC CAGAGGTTAC 
CTGAAAACAA GTCTTTGTGT GCTGGGGCCT GGGGATGAAG CGCCTCTGGA GAGAAAAGAG 
CCCTCTGAAG ACAAGGAGGA CATTGAAAGC AACCTGCTCC GGCCCACAGG CGTGGCCCTG 
CGAGGAGCCC ACTTCTGCCT GAAGGTCTTC CGGGCCGAGG ACTTGCCACA GATGGACGAT 
GCTGTGATGG ACAACGTGAA ACAGATCTTT GGCTTTGAGA GTAACAAGAA GAACTTGGTG 
GACCCCTTTG TGGAGGTCAG CTTTGCGGGG AAAATGCTCT GCAGCAAGAT CTTGGAGAAG 
ACGGCCAACC CTCAGTGGAA CCAGAACATC ACACTGCCTG CCATGTTTCC CTCCATGTGT 
GAAAAAATGA GGATTCGTGT CATAGACTGG GATCGCCTCA CTCACAATGA CATCGTGGCT 
ACCGCCGACC TGAGTATGTC GAAAATCTCT GCCCCTGGAG GAGACATAGA AGAGGAGACT 
GCAGGTGCCG TCAAGCCTTC GAAAGTCTCA GACCTGGATG ACCACCTGGG CTTCCTCCCC 
ACTTTTGGGC CCTGCTACAT CAACCTCTAT GGCAGTCCCA GAGAGTTCAC GGGCTTCCTG 
GACCCCTACG CAGAGCTCAA CACAGGCAAG GGGGAAGGTG TGGCTTATCG TGGCCGGCTT 
CTGCTTTCCC TGGAGACCAA GCTGGTGGAG CACAGTGAGC AGAAGGTGGA GGACCTTCCT 
GCGGATGACA TCCTCCGGGT GGAGAAGTAC CTTAGGAGGC GCAAGTACTC CCTGTTTGCG 
GCCTTCTACT CGGCCACCAT GCTGCAGGAT GTGGATGACG CCATCCAGTT TGAGGTCAGC 
ATCGGGAACT ATGGGAACAA GTTCGACATG ACCTGCCTGC CGCTGGCCTC CACCACTCAG 
TATAGCCGTG CAGTCTTTGA CGGGTGCCAC TACTACTACC TACCCTGGGG CAACGTGAAA 
CCTGTGGTGG TGCTGTCATC CTACTGGGAG GACATCAGCC ATAGAATCGA GACTCAGAAC 
CAGCTGCTTG GGATTGCTGA CCGGCTGGAA GCTGGCCTGG AGCAGGTCCA CCTGGCCCTG 
AAGGCGCAGT GCTCCACGGA GGACGTGGAC TCACTGGTGG CTCAGATGAT GGATGAGCTC 
ATCGCAGGCT GCAGCCAGCC TCTGGGTGAT ATCCATGAGA TACCCTCTGC CACCCACCTG 
GACGAGTACC TGTACCGGCT GCGCACCCGT CACCTGAGCC AAATCACGGA GGCTGCCCTG 
GCCCTGAAGC TTGGCCACAG TGAGCTCCCT GCTGCTCTGG ATCAGGCTGA GGACTGGCTC 
CTGCGTCTGC GTGCCCTCGC TGAGGAGCCC CAGAACAGCC TGCCGGATAT CATCATCTGG 
ATGCTGCAGG GAGACAAGCG TGTGGCATAC CAGCGGGTGC CCGCCCACCA AGTCCTCTTC 
TCCCGGCGGG GTGCCAACTT CTGTGGCAAG AATTGTGGGA AGTTGCAGAC AATCTTTCTG 
AAATATCCGA TGGAGAAGGT GCCTGGGGCC CGGATGCCAG TGCAGATACG GGTCAAGCTG 
TGGTTTGGGC TCTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG GAAGCTGTCT 
GTCTTTGCTG AAACCTATGA GAACCAGACC AAGTTGGCCC TGGTCGGGAA CTGGGGCACA 
ACGGGCCTCA CCTACCCCAA GTTTTCTGAC GTCACCGGCA AGATCAAGCT ACCCAAAGAG 
AGCTTCCGCC CCTCGGCCGG CTGGACCTGG GCTGGAGATT GGTTCGTGTG TCCGGAGAAG 
ACCCTGCTCC ATGACATGGA CGCCGGTCAC CTGAACTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TTCCCGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC CGATGTGAAC 
GGGGAGAAGG TGCTTCCCAA AGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAGGAATGGT CCACAGACCT CAACCGGGCT GTCGATGAGC AAGGCTGGGA GTATAGCATC 
ACCATCCCCC CGGACCGGAA GCCGAAGCAC TGGGTCCCTG CTGAGAAGAT GTACTACACA 
CACCGACGGC GGCGCTGGGT GCGCCTGCGG AGGAGGGATC TCAGCCAGAT GGAAGCGCTG 
AAGAGGCATA GGCAGGCGGA GGCAGAGGGC GAGGGCTGGG AGTACGCCTC TCTTTTTGGC 
TGGAAGTTCC ACCTCGAGTA CCGCAAGACA GACGCCTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAGC CCCTGGAGAA GACGGGGCCT GCAGCTGTGT TTGCCCTCGA GGGGGCCCTG 
GGCGGCGTGG TGGACGACAA GAGTGAAGAT TCCATGTCCG TCTCCACCTT GAGCTTTGGT 
GTGAACAGAC CCACAATTTC CTGCATATTT GACTATGGGA ACCGCTACCA TCTACGCTGC 
TACATGTACC AGGCCCGGGA CCTGGCTGCG ATGGACAAGG ACTCTTTTTC TGATCCCTAT 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACGGTGG TGGTGAAGAA CACCCTTAAC 
CCCACCTGGG ACCAGACGCT CATCTTCTAC GAGATCGAGA TCTTCGGCGA GCCGGCCACA 
GTTGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTACG ACCATGACAC TTACGGCGCA 
GACGAGTTTA TGGGCCGCTG CATCTGTCAG CCGAGTTTGG AACGGACGCC CCGACTGGCC 
TGGTTCCCAC TGACCAGGGG CAGCCAGCCG ACGGGGGAGC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AGAAGCCGGC CATCCACCAT ATTCCTGGTT TTGAGGTGCA GGAGACATCG 
AGGATCCTGG ATGAGCTGTG TGCTCCTGCC TTCTTCCTCC AGGGCCTTCT CCAAACGTCT 
GAGGACACAG ACCTGCCCTA CCCACCACCC CAGAGGGAGG CCAACATCTA CATGGTTCCT 
CAGAACATCA AGCCAGCGCT CCAGCGTACC GCCATCGAGG TCCTGGCATG GGGCCTGCGG 
AACATGAAGA GTTACCAGCT GGCCAGCATC TCCTCCCCGA GCCTTGTGAT AGAGTGTGGG 
GGCCAGACGG TGCAGTCCTG TGTCATCAGG AACCTCCGGA AGAACCCCAA CTTTGACATC 
TGTACCCTCT TCATGGAAGT GATGCTGCCC AGGGAGGAGC TCTACTGCCC CCCCATCACC 
ATCAAGGTCA TCGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGCTCCCTGG AGAGCTTCCT GTGTGACCCC TACTCGGCGG AGAGTCCATC CCCACAGGGT 
GGCCCAGACG ATGTGAGCCT ACTCAGTCCT GGGGAAGACG TGCTCATCGA CATTGATGAC 
AAGGAGCCCC TCATCCCCAT CCAGCTTGCA GACGGTCTGT CGAGCTTGGC CCCTACTAAC 
ACGGCTTCTC CTCCATCCAG TCCTCATGAG GAAGAGTTTA TCGATTGGTG GAGCAAATTC 
TTTGCCTCCA TAGGGGAGAG GGAAAAGTGC GGCTCCTACT TGGAGAAGGA TTTTGACACC 
CTGAAGGTCT ATGACACACA GCTGGAGAAT GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTGTA CCGGGGCAAG ACGCAGGAGG AGACAGAAGA TCCATCTGTC 
ATTGGCGAAT TTAAGGGCCT CTTCAAAATT TATCCCCTCC CAGAAGACCC AGCCATCCCC 
ATGCCCCCAA GACAGTTCCA CCAGCTGGCC GCGCAGGGAC CCCAGGAGTG CTTGGTCCGT 
ATCTACATTG TCCGAGCATT TGGCCTGCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATCCCCTGC 
ACGTTGGAGC CTGTGTTTGG AAAGATGTTT GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGG TCACTCTCTA CGACTATGAC CTCCTCTCCA AGGATGAAAA GATTGGTGAG 
ACGGTCATCG ACCTGGAGAA CAGGCTGCTG TCCAAGTTTG CGGCTCGCTG TGGACTCCCG 
CAGACCTACT GTGTCTCTGG ACCGAACCAG TGGCGGGACC AGCTCCGCCC CTCCCAGCTC 
CTCCACCTCT TCTGCCAGCA GCGCAGAGTC AAGGCGCCTG TGTACCGGAC AGACCGTGTG 
ATGTTTCAGG ATAAAGAATA CTCCATTGAA GAAATAGAGG CTGGCAGGAT CCCAAACCCA 
CACCTGGGCC CCGTGGAGGA GCGTCTGGCT CTGCACGTGC TTCAGCAGCA GGGCCTGGTC 
CCAGAGCACG TGGAGTCACG GCCCCTCTAC AGCCCCCTGC AGCCAGACAT TGAGCAGGGG 
AAGCTGCAGA TGTGGGTCGA CCTGTTTCCG AAGGCCCTGG GGCGCCCTGG ACCTCCCTTC 
AACATCACCC CACGGAGAGC CAGAAGGTTT TTCCTGCGTT CTATTATCTG GAATACAAGA 
GATGTGATCC TGGATGACCT GAGCCTCACG GGGGAGAAGA TGAGCGACAT TTATGTGAAA 
GGTTGGATGA TTGGCTTTGA AGAACACAAG CAAAAGACAG ACGTGCATTA TCGCTCCCTG 
GGAGGTGAAG GCAACTTCAA CTGGAGGTTC ATTTTCCCCT TCGACTACCT GCCGGCTGAG 
CAAGTCTGTA CCGTTGCCAA GAAGGATGCC TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGATATT CCAGATCTGG GACAATGACA AGTTCTCCTT TGATGATTTT 
CTGGGCTCCC TGCAGCTCGA TCTCAACCGC ATGCCCAAGC CAGCCAAGAC AGCCGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCAGAAT GGTTTGTGTC CCTTTTTGAG 
CAGAAAACAG TGAAGGGCTG GTGGCCTTGT GTAGCAGAAG AGGGTGAGAA GAAGATACTG 
GCGGGCAAGC TGGAAATGAC CTTGGAGATT GTAACAGAGA GTGAGCATGA GGAGCGGCCC 
GCTGGCCAGG GCAGGGATGA GCCCAACATG AACCCTAAAC TTGAGGACCC AAGGCGCCCC 
GACACCTCCT TCCTGTGGTT CACCTCCCCA TACAAGACCA TGAAGTTCAT CCTGTGGCGG 
CGTTTCCGGT GGGCCATCAT CCTCTTTATC ATCCTCTTCA TCCTGCTGCT GTTCCTGGCC 
ATCTTCATCT ACGCCTTCCC GAACTATGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	704636
Full Name	dysferlin
Gene Type	protein-coding
Organism	Macaca mulatta(Rhesus monkey)

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Macaca mulatta(Rhesus monkey)

Gene

Clones

gRNAs

Related articles in PubMed

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