ARFGEF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARFGEF1
Entrez Gene ID	281022
Full Name	ADP ribosylation factor guanine nucleotide exchange factor 1
Synonyms	BIG1
General protein information	Preferred Names ADP ribosylation factor guanine nucleotide exchange factor 1 Names brefeldin A-inhibited guanine nucleotide-exchange protein 1 ADP-ribosylation factor guanine nucleotide-exchange factor 1 (brefeldin A-inhibited) brefeldin A-inhibited GEP 1 p200 ARF guanine nucleotide exchange factor p200 ARF-GEP1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_173997.2	NP_776422.1	brefeldin A-inhibited guanine nucleotide-exchange protein 1
NM_173997.3	NP_776422.2	brefeldin A-inhibited guanine nucleotide-exchange protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGEF1	NP_006412.2
	ARFGEF1	XP_001096475.1
Canis lupus familiaris (dog)	ARFGEF1	XP_859322.2
Mus musculus (house mouse)	Arfgef1	NP_001095900.1
Eremothecium gossypii	AGOS_AGL147C	NP_986520.2
Neurospora crassa	NCU07658	XP_962785.2
Arabidopsis thaliana (thale cress)	EDA10	NP_171698.1
Danio rerio (zebrafish)	arfgef1	XP_003201354.2
Drosophila melanogaster (fruit fly)	sec71	NP_609675.2
Bos taurus (cattle)	ARFGEF1	NP_776422.1
Schizosaccharomyces pombe (fission yeast)	sec72	NP_594555.1
Arabidopsis thaliana (thale cress)	AT3G60860	NP_191645.1
Oryza sativa (rice)	Os03g0246800	NP_001049545.1
Pan troglodytes (chimpanzee)	ARFGEF1	XP_001162263.1
Gallus gallus (chicken)	ARFGEF1	XP_418283.3
Magnaporthe oryzae (rice blast fungus)	MGG_14173	XP_003720355.1
Xenopus tropicalis (tropical clawed frog)	arfgef1	XP_002935368.2
Rattus norvegicus (Norway rat)	Arfgef1	NP_001263985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008906	XP_319652.3
Caenorhabditis elegans (roundworm)	agef-1	NP_001129800.1
Saccharomyces cerevisiae (baker's yeast)	SEC7	NP_010454.3
Kluyveromyces lactis	KLLA0D17358g	XP_453828.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning and expression of a cDNA encoding a bovine brain brefeldin A-sensitive guanine nucleotide-exchange protein for ADP-ribosylation factor.
Morinaga N, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America94(24)12926-31(1997 Nov)

Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain.
Morinaga N, Tsai SC, Moss J, Vaughan M
Proceedings of the National Academy of Sciences of the United States of America93(23)12856-60(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ARFGEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARFGEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa238847	NM_173997.3 Latest version!	Bos taurus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa42557	NM_173997.2 Latest version!	Bos taurus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARFGEF1 ( NM_173997.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776422.2 Bos taurus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Price & Availability↑

CloneID	OBa238847
Clone ID Related Accession (Same CDS sequence)	NM_173997.3
Accession Version	NM_173997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02038707.1, DAAA02038702.1 and DAAA02038700.1. On Jan 19, 2018 this sequence version replaced NM_173997.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF023451.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAG CAGAGTCCTC CTCACGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCCTCCCCT TTGAATTGGC ATGCCAGTCC AAATGTCCCC GCATAGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TTTGTGGCTG TTTCCAAGGT 
CCTCAAACAG ATGAAGGAGT TCAGTTACAA ATAATAAAGG CTTTACTAAC TGCAGTAACA 
TCTCAACACA TAGAAATTCA TGAAGGAACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATATATCTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGTTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TGCAGGAAGC CAAACAAATG 
GAAAAAGAAA GGCATCGACA GCACCACCAT CTGTTGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAACTCAG ATACTTGCCA CCTCAGACTG TTGATCACAT ACCCCAAGAA 
CATGAAGGGG ACCTTGATCC CCAGACAAAT GATGTGGATA AAAGCCTTCA GGATGATACA 
GAGCCCGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCCGATCAG 
GCAACTGCAG CTGAAACATT GTCTAAAAAT GATATATTAT ATGATGGAGA AAACCATGAC 
TGTGAGGAAA AGCCACAGGA CATTGTACAG AGCATTGTAG AAGAAATGGT GAACATCGTT 
GTTGGAGATA CGGGAGAAAG GACTACCATA AATGTAAGTG CAGATGGCAA CAATGGAACC 
ATAGAGGATG GTAGCGACAG TGAAAATATT CAAGCAAACG GAATTCCAGG AACACCAATT 
TCTGTTGCTT ATACACCGTC CTTACCTGAT GACAGATTGT CTGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTATCGGAT 
GGACCACCGG ATCCAAAGTC TCATGAGTTA CGATCCAAGA TTCTTTCACT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATAAAGCAGT ATCTGTGTGT TGCACTCTCA AAAAATGGAG TTTCATCTGT CCCAGAGGTT 
TTTGAGCTGT CTCTTTCCAT ATTTCTTACT TTGTTATCAA ACTTCAAAAC ACATCTGAAG 
ATGCAGATTG AGGTGTTCTT TAAAGAAATT TTCCTATATA TTTTGGAGAC CTCTACCAGC 
TCATTTGACC ACAAATGGAT GGTTATTCAG ACATTAACAA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATATATGT AAACTATGAT TGTGACTTAA ATGCAGCAAA CATATTTGAA 
CGACTAGTAA ATGATCTGTC AAAAATTGCC CAAGGACGAG GCAGTCAGGA ACTTGGTATG 
AGTAATGTTC AGGAACTGAG CCTAAGGAAA AAAGGTTTAG AATGCTTAGT GTCAATTTTG 
AAGTGCATGG TTGAATGGAG CAAGGATCAG TATGTGAATC CCAACTCACA GACGACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGACG AGTGAAATGA AACACCCAGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACGTCATCAT CAGGAATAGG CAGCTACAGC 
ACACAGATGT CTGGCACTGA TAATCCGGAG CAGTTCGAAG TCCTCAAGCA GCAGAAAGAA 
ATAATAGAAC AAGGGATAGA CTTATTTACT AAGAAACCAA AGAGAGGAAT CCAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACCCCT GAAGACATTG CCCAGTTCTT ACACCAAGAG 
GAAAGATTAG ACTCAACTCA AGTGGGTGAA TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCTTATGT GGACCAACAT GACTTTTCAG GAAAAGATTT TGTTTCAGCC 
CTTCGTATGT TTCTGGAAGG ATTCCGTCTT CCAGGGGAAG CTCAGAAAAT TGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATATCTAGAA TGCAACCAAG GGCAAACCCT TTTTGCCAGT 
GCAGATACTG CTTATGTTTT GGCTTATTCA ATTATCATGC TGACCACAGA TCTTCATAGT 
CCACAGGTTA AAAATAAAAT GACCAAGGAA CAGTACATTA AGATGAATAG AGGCATCAAT 
GACAGTAAAG ATCTCCCTGA AGAGTATCTG TCAGCCATCT ATAATGAAAT AGCTGGAAAA 
AAGATATCAA TGAAAGAAAC GAAGGAGCTG ACAATCCCTG CAAAGTCAAG TAAACAGAAC 
GTAGCCAGTG AAAAACAAAG AAGACTCCTG TATAACTTAG AGATGGAACA GATGGCCAAG 
ACCGCTAAAG CTCTCATGGA GGCTGTGAGC CACGTTCAGG CACCTTTCAC GAGTGCAACA 
CATTTGGAGC ATGTGAGACC CATGTTTAAG CTGGCTTGGA CCCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGACTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAAAGAGA TGCATACGTC 
CAGGCGCTGG CAAGATTCAC CTTACTCACA GTGAGTTCTG GGATTACTGA AATGAAGCAG 
AAGAACATTG ACACAATCAA AACGCTTATC ACAGTGGCTC ATACAGATGG AAATTACTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGC TGGAGCTTGC TCAACTTATA 
GGAACTGGAG TGAAGCCTCG GTACATTTCT GGAACAGTAC GAGGCAGAGA AGGATCACTT 
ACTGGAGCAA AAGATCAGGC TCCTGATGAA TTTGTGGGGC TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AGCAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAAAGT 
GTTGTTGTTG CTGTAGATAG AATATTTACA GGGTCCACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC TACTACGCAT 
CCAAGAATGT TTAGTCTGCA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTGCAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGCTGT 
AATCCTAATG AAGATGTAGC TATTTTCGCA GTAGACTCCC TGAGGCAGCT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAATTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGCG ATATGGTTGT ACGGTGTATA 
GCGCAGATGG TTAACTCTCA AGCTGCTAAC ATTCGGTCCG GATGGAAAAA CATCTTCTCT 
GTATTTCATC TTGCTGCATC GGATCAAGAT GAAAGCATAG TTGAGCTTGC ATTCCAGACA 
ACAGGACATA TTGTCACCCT TGTGTTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAATGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGATACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATACG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAGT ACACAAGTGA TGACATGAAT GTGGCACCTG AAGACAGAGT GTGGGTGAGA 
GGCTGGTTCC CAATTCTCTT CGAGTTATCA TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CTTACGGCTA CACTTATGAA 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTA 
CCAGAACAGC AGACAGAGAA AGCCGAATGG ATGACGACAA CATGCAATCA TGCACTCTAT 
GCGATCTGTG ACGTGTTCAC CCAGTACTTA GAAGTGCTCA GTGATGTGCT TTTGGACGAC 
ATTTTTGCCC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTGGC ACGATCTGGT 
ACAAACTGCT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTCACACT AGAAATCTGG 
GATAAAACGT GCAACTGCAC ACTGGACATC TTCAAAACCA CAATCCCACA TGCGCTCTTG 
ACCTGGCGTC CCATTTCTGG AGAAACTGCT CCCCCAACCC CATCTCCTGT GAGTGAAAAC 
CAGTTGGATA CAATATCACA GAAATCTGTA GATATTCATG ATTCTATTCA ACCAAGATCT 
GCAGATAATA GACAGCAAGC TCCGTTGGCT TCTGTCTCTA CTGTGAATGA AGAAATCAGC 
AAAATTAAAC CCACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCCGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TCGTGTTCTT CCCCGCCACA 
AGTAGGAAGG AGGACGCGGA AAACTTAGCC GCGGCGCAGA GAGACGCTGT GGACTTTGAT 
GTTCGAGTGG ACACTCAAGA CCAAGGAATG TATCGCTTTT TAACATCACA ACAACTCTTC 
AAGCTCCTGG ACTGCTTGTT AGAGTCACAC AGATTTGCAA AAGCTTTCAA CTCCAACAAC 
GAACAGAGGA CGGCTCTGTG GAAGGCGGGC TTCAAGGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CGAGCAGCCT GGCCTGCGGG CTGCGCATTC TCTTCCGGAT GTACACGGAC 
GAGAGCCGGG CCAGCGCCTG GGAGGAGGTC CAGCAGAGGC TTTTAAATGT CTGCAGCGAA 
GCACTCAGTT ACTTTCTTAC TCTAACGTCG GAAAGTCATC GGGAAGCCTG GACTAACTTA 
CTGCTCTTAT TTCTGACTAA AGTCCTGAAG ATCAGCGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGAGAATT GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776422.2
CDS	158..5707
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHIPQEHEGDLDPQTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNDILYDGENHDCEEKPQDIVQSIVEEMVNIVVGDTGERTTINVSADGNNGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFRTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQETSEMKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFTKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPAKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGAKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGYTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPISGETAPPTPSPVSENQLDTISQKSVDIHDSIQPRSADNRQQAPLASVSTVNEEISKIKPTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSRKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDESRASAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	NM_173997.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173997.3

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAG CAGAGTCCTC CTCACGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCCTCCCCT TTGAATTGGC ATGCCAGTCC AAATGTCCCC GCATAGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TTTGTGGCTG TTTCCAAGGT 
CCTCAAACAG ATGAAGGAGT TCAGTTACAA ATAATAAAGG CTTTACTAAC TGCAGTAACA 
TCTCAACACA TAGAAATTCA TGAAGGAACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATATATCTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGTTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TGCAGGAAGC CAAACAAATG 
GAAAAAGAAA GGCATCGACA GCACCACCAT CTGTTGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAACTCAG ATACTTGCCA CCTCAGACTG TTGATCACAT ACCCCAAGAA 
CATGAAGGGG ACCTTGATCC CCAGACAAAT GATGTGGATA AAAGCCTTCA GGATGATACA 
GAGCCCGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCCGATCAG 
GCAACTGCAG CTGAAACATT GTCTAAAAAT GATATATTAT ATGATGGAGA AAACCATGAC 
TGTGAGGAAA AGCCACAGGA CATTGTACAG AGCATTGTAG AAGAAATGGT GAACATCGTT 
GTTGGAGATA CGGGAGAAAG GACTACCATA AATGTAAGTG CAGATGGCAA CAATGGAACC 
ATAGAGGATG GTAGCGACAG TGAAAATATT CAAGCAAACG GAATTCCAGG AACACCAATT 
TCTGTTGCTT ATACACCGTC CTTACCTGAT GACAGATTGT CTGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTATCGGAT 
GGACCACCGG ATCCAAAGTC TCATGAGTTA CGATCCAAGA TTCTTTCACT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCAGGACAA ATGAGATGTT TATTAATGCT 
ATAAAGCAGT ATCTGTGTGT TGCACTCTCA AAAAATGGAG TTTCATCTGT CCCAGAGGTT 
TTTGAGCTGT CTCTTTCCAT ATTTCTTACT TTGTTATCAA ACTTCAAAAC ACATCTGAAG 
ATGCAGATTG AGGTGTTCTT TAAAGAAATT TTCCTATATA TTTTGGAGAC CTCTACCAGC 
TCATTTGACC ACAAATGGAT GGTTATTCAG ACATTAACAA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATATATGT AAACTATGAT TGTGACTTAA ATGCAGCAAA CATATTTGAA 
CGACTAGTAA ATGATCTGTC AAAAATTGCC CAAGGACGAG GCAGTCAGGA ACTTGGTATG 
AGTAATGTTC AGGAACTGAG CCTAAGGAAA AAAGGTTTAG AATGCTTAGT GTCAATTTTG 
AAGTGCATGG TTGAATGGAG CAAGGATCAG TATGTGAATC CCAACTCACA GACGACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGACG AGTGAAATGA AACACCCAGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACGTCATCAT CAGGAATAGG CAGCTACAGC 
ACACAGATGT CTGGCACTGA TAATCCGGAG CAGTTCGAAG TCCTCAAGCA GCAGAAAGAA 
ATAATAGAAC AAGGGATAGA CTTATTTACT AAGAAACCAA AGAGAGGAAT CCAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACCCCT GAAGACATTG CCCAGTTCTT ACACCAAGAG 
GAAAGATTAG ACTCAACTCA AGTGGGTGAA TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCTTATGT GGACCAACAT GACTTTTCAG GAAAAGATTT TGTTTCAGCC 
CTTCGTATGT TTCTGGAAGG ATTCCGTCTT CCAGGGGAAG CTCAGAAAAT TGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATATCTAGAA TGCAACCAAG GGCAAACCCT TTTTGCCAGT 
GCAGATACTG CTTATGTTTT GGCTTATTCA ATTATCATGC TGACCACAGA TCTTCATAGT 
CCACAGGTTA AAAATAAAAT GACCAAGGAA CAGTACATTA AGATGAATAG AGGCATCAAT 
GACAGTAAAG ATCTCCCTGA AGAGTATCTG TCAGCCATCT ATAATGAAAT AGCTGGAAAA 
AAGATATCAA TGAAAGAAAC GAAGGAGCTG ACAATCCCTG CAAAGTCAAG TAAACAGAAC 
GTAGCCAGTG AAAAACAAAG AAGACTCCTG TATAACTTAG AGATGGAACA GATGGCCAAG 
ACCGCTAAAG CTCTCATGGA GGCTGTGAGC CACGTTCAGG CACCTTTCAC GAGTGCAACA 
CATTTGGAGC ATGTGAGACC CATGTTTAAG CTGGCTTGGA CCCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGACTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAAAGAGA TGCATACGTC 
CAGGCGCTGG CAAGATTCAC CTTACTCACA GTGAGTTCTG GGATTACTGA AATGAAGCAG 
AAGAACATTG ACACAATCAA AACGCTTATC ACAGTGGCTC ATACAGATGG AAATTACTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGC TGGAGCTTGC TCAACTTATA 
GGAACTGGAG TGAAGCCTCG GTACATTTCT GGAACAGTAC GAGGCAGAGA AGGATCACTT 
ACTGGAGCAA AAGATCAGGC TCCTGATGAA TTTGTGGGGC TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AGCAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAAAGT 
GTTGTTGTTG CTGTAGATAG AATATTTACA GGGTCCACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC TACTACGCAT 
CCAAGAATGT TTAGTCTGCA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTGCAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGCTGT 
AATCCTAATG AAGATGTAGC TATTTTCGCA GTAGACTCCC TGAGGCAGCT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAATTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGCG ATATGGTTGT ACGGTGTATA 
GCGCAGATGG TTAACTCTCA AGCTGCTAAC ATTCGGTCCG GATGGAAAAA CATCTTCTCT 
GTATTTCATC TTGCTGCATC GGATCAAGAT GAAAGCATAG TTGAGCTTGC ATTCCAGACA 
ACAGGACATA TTGTCACCCT TGTGTTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAATGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGATACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATACG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAGT ACACAAGTGA TGACATGAAT GTGGCACCTG AAGACAGAGT GTGGGTGAGA 
GGCTGGTTCC CAATTCTCTT CGAGTTATCA TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CTTACGGCTA CACTTATGAA 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTA 
CCAGAACAGC AGACAGAGAA AGCCGAATGG ATGACGACAA CATGCAATCA TGCACTCTAT 
GCGATCTGTG ACGTGTTCAC CCAGTACTTA GAAGTGCTCA GTGATGTGCT TTTGGACGAC 
ATTTTTGCCC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTGGC ACGATCTGGT 
ACAAACTGCT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTCACACT AGAAATCTGG 
GATAAAACGT GCAACTGCAC ACTGGACATC TTCAAAACCA CAATCCCACA TGCGCTCTTG 
ACCTGGCGTC CCATTTCTGG AGAAACTGCT CCCCCAACCC CATCTCCTGT GAGTGAAAAC 
CAGTTGGATA CAATATCACA GAAATCTGTA GATATTCATG ATTCTATTCA ACCAAGATCT 
GCAGATAATA GACAGCAAGC TCCGTTGGCT TCTGTCTCTA CTGTGAATGA AGAAATCAGC 
AAAATTAAAC CCACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCCGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TCGTGTTCTT CCCCGCCACA 
AGTAGGAAGG AGGACGCGGA AAACTTAGCC GCGGCGCAGA GAGACGCTGT GGACTTTGAT 
GTTCGAGTGG ACACTCAAGA CCAAGGAATG TATCGCTTTT TAACATCACA ACAACTCTTC 
AAGCTCCTGG ACTGCTTGTT AGAGTCACAC AGATTTGCAA AAGCTTTCAA CTCCAACAAC 
GAACAGAGGA CGGCTCTGTG GAAGGCGGGC TTCAAGGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CGAGCAGCCT GGCCTGCGGG CTGCGCATTC TCTTCCGGAT GTACACGGAC 
GAGAGCCGGG CCAGCGCCTG GGAGGAGGTC CAGCAGAGGC TTTTAAATGT CTGCAGCGAA 
GCACTCAGTT ACTTTCTTAC TCTAACGTCG GAAAGTCATC GGGAAGCCTG GACTAACTTA 
CTGCTCTTAT TTCTGACTAA AGTCCTGAAG ATCAGCGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGAGAATT GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARFGEF1 ( NM_173997.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776422.1 Bos taurus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

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CloneID	OBa42557
Clone ID Related Accession (Same CDS sequence)	NM_173997.2
Accession Version	NM_173997.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5550bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-21
Organism	Bos taurus(cattle)
Product	brefeldin A-inhibited guanine nucleotide-exchange protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF023451.1. On Jun 3, 2003 this sequence version replaced NM_173997.1. ##Evidence-Data-START## Transcript exon combination :: AF023451.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAG CAGAGTCCTC CTCACGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCCTCCCCT TTGAATTGGC ATGCCAGTCC AAATGTCCCC GCATAGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TTTGTGGCTG TTTCCAAGGT 
CCTCAAACAG ATGAAGGAGT TCAGTTACAA ATAATAAAGG CTTTACTAAC TGCAGTAACA 
TCTCAACACA TAGAAATTCA TGAAGGAACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATATATCTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGTTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TGCAGGAAGC CAAACAAATG 
GAAAAAGAAA GGCATCGACA GCACCACCAT CTGTTGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAACTCAG ATACTTGCCA CCTCAGACTG TTGATCACAT ACCCCAAGAA 
CATGAAGGGG ACCTTGATCC CCAGACAAAT GATGTGGATA AAAGCCTTCA GGATGATACA 
GAGCCCGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCCGATCAG 
GCAACTGCAG CCGAAACATT GTCTAAAAAT GATATATTAT ATGATGGAGA AAACCATGAC 
TGTGAGGAAA AGCCACAGGA CATTGTACAG AGCATTGTAG AAGAAATGGT GAACATCGTT 
GTTGGAGATA CGGGAGAAAG GACTACCATA AATGTAAGTG CAGATGGCAA CAATGGAACC 
ATAGAGGATG GTAGCGACAG TGAAAATATT CAAGCAAACG GAATTCCAGG AACACCAATT 
TCTGTTGCTT ATACACCGTC CTTACCTGAT GACAGATTGT CTGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTATCGGAT 
GGACCACCGG ATCCAAAGTC TCATGAGTTA CGATCCAAGA TTCTTTCACT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCGGGACAA ATGAGATGTT TATTAATGCT 
ATAAAGCAGT ATCTGTGTGT TGCACTCTCA AAAAATGGAG TTTCATCTGT CCCAGAGGTT 
TTTGAGCTGT CTCTTTCCAT ATTTCTTACT TTGTTATCAA ACTTCAAAAC ACATCTGAAG 
ATGCAGATTG AGGTGTTCTT TAAAGAAATT TTCCTATATA TTTTGGAGAC CTCTACCAGC 
TCATTTGACC ACAAATGGAT GGTTATTCAG ACATTAACAA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATATATGT AAACTATGAT TGTGACTTAA ATGCAGCAAA CATATTTGAA 
CGACTAGTAA ATGATCTGTC AAAAATTGCC CAAGGACGAG GCAGTCAGGA ACTTGGTATG 
AGTAATGTTC AGGAACTGAG CCTAAGGAAA AAAGGTTTAG AATGCTTAGT GTCAATTTTG 
AAGTGCATGG TTGAATGGAG CAAGGATCAG TATGTGAATC CCAACTCACA GACGACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGACG AGTGAAATGA AACACCCAGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACGTCATCAT CAGGAATAGG CAGCTACAGC 
ACACAGATGT CTGGCACTGA TAATCCGGAG CAGTTCGAAG TCCTCAAGCA GCAGAAAGAA 
ATAATAGAAC AAGGGATAGA CTTATTTACT AAGAAACCAA AGAGAGGAAT CCAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACCCCT GAAGACATTG CCCAGTTCTT ACACCAAGAG 
GAAAGATTAG ACTCAACTCA AGTGGGTGAA TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCTTATGT GGACCAACAT GACTTTTCAG GAAAAGATTT TGTTTCAGCC 
CTTCGTATGT TTCTGGAAGG ATTCCGTCTT CCAGGGGAAG CTCAGAAAAT TGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATATCTAGAA TGCAACCAAG GGCAAACCCT TTTTGCCAGT 
GCAGATACTG CTTATGTTTT GGCTTATTCA ATTATCATGC TGACCACAGA TCTTCATAGT 
CCACAGGTTA AAAATAAAAT GACCAAGGAA CAGTACATTA AGATGAATAG AGGCATCAAT 
GACAGTAAAG ATCTCCCTGA AGAGTATCTG TCAGCCATCT ATAATGAAAT AGCTGGAAAA 
AAGATATCAA TGAAAGAAAC GAAGGAGCTG ACAATCCCTG CAAAGTCAAG TAAACAGAAC 
GTAGCCAGTG AAAAACAAAG AAGACTCCTG TATAACTTAG AGATGGAACA GATGGCCAAG 
ACCGCTAAAG CTCTCATGGA GGCTGTGAGC CACGTTCAGG CACCTTTCAC GAGTGCAACA 
CATTTGGAGC ATGTGAGACC CATGTTTAAG CTGGCTTGGA CCCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGACTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAAAGAGA TGCATACGTC 
CAGGCGCTGG CAAGATTCAC CTTACTCACA GTGAGTTCTG GGATTACTGA AATGAAGCAG 
AAGAACATTG ACACAATCAA AACGCTTATC ACAGTGGCTC ATACAGATGG AAATTACTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGC TGGAGCTTGC TCAACTTATA 
GGAACTGGAG TGAAGCCTCG GTACATTTCT GGAACAGTAC GAGGCAGAGA AGGATCACTT 
ACTGGAGCAA AAGATCAGGC TCCTGATGAA TTTGTGGGGC TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AGCAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAAAGT 
GTTGTTGTTG CTGTAGATAG AATATTTACA GGGTCCACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC TACTACGCAT 
CCAAGAATGT TTAGTCTGCA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTGCAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGCTGT 
AATCCTAATG AAGATGTAGC TATTTTCGCA GTAGACTCCC TGAGGCAGCT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAATTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGCG ATATGGTTGT ACGGTGTATA 
GCGCAGATGG TTAACTCTCA AGCTGCTAAC ATTCGGTCCG GATGGAAAAA CATCTTCTCT 
GTATTTCATC TTGCTGCATC GGATCAAGAT GAAAGCATAG TTGAGCTTGC ATTCCAGACA 
ACAGGACATA TTGTCACCCT TGTGTTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAATGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGATACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATACG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAGT ACACAAGTGA TGACATGAAT GTGGCACCTG AAGACAGAGT GTGGGTGAGA 
GGCTGGTTCC CAATTCTCTT CGAGTTATCA TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CTTACGGCTA CACTTATGAA 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTA 
CCAGAACAGC AGACAGAGAA AGCCGAATGG ATGACGACAA CATGCAATCA TGCACTCTAT 
GCGATCTGTG ACGTGTTCAC CCAGTACTTA GAAGTGCTCA GTGATGTGCT TTTGGACGAC 
ATTTTTGCCC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTGGC ACGATCTGGT 
ACAAACTGCT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTCACACT AGAAATCTGG 
GATAAAACGT GCAACTGCAC ACTGGACATC TTCAAAACCA CAATCCCACA TGCGCTCTTG 
ACCTGGCGTC CCATTTCTGG AGAAACTGCT CCCCCAACCC CATCTCCTGT GAGTGAAAAC 
CAGTTGGATA CAATATCACA GAAATCTGTA GATATTCATG ATTCTATTCA ACCAAGATCT 
GCAGATAATA GACAGCAAGC TCCGTTGGCT TCTGTCTCTA CTGTGAATGA AGAAATCAGC 
AAAATTAAAC CCACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCCGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TCGTGTTCTT CCCCGCCACA 
AGTAGGAAGG AGGACGCGGA AAACTTAGCC GCGGCGCAGA GAGACGCTGT GGACTTTGAT 
GTTCGAGTGG ACACTCAAGA CCAAGGAATG TATCGCTTTT TAACATCACA ACAACTCTTC 
AAGCTCCTGG ACTGCTTGTT AGAGTCACAC AGATTTGCAA AAGCTTTCAA CTCCAACAAC 
GAACAGAGGA CGGCTCTGTG GAAGGCGGGC TTCAAGGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CGAGCAGCCT GGCCTGCGGG CTGCGCATTC TCTTCCGGAT GTACACGGAC 
GAGAGCCGGG CCAGCGCCTG GGAGGAGGTC CAGCAGAGGC TTTTAAATGT CTGCAGCGAA 
GCACTCAGTT ACTTTCTTAC TCTAACGTCA GAAAGTCATC GGGAAGCCTG GACTAACTTA 
CTGCTCTTAT TTCTGACTAA AGTCCTGAAG ATCAGCGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGAGAATT GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776422.1
CDS	158..5707
Translation	MYEGKKTKNMFLTRALEKILADKEVKKAHHSQLRKACEVALEEIKAETEKQSPPHGEAKAGSSTLPPVKSKTNFIEADKYFLPFELACQSKCPRIVSTSLDCLQKLIAYGHLTGNAPDSTTPGKKLIDRIIETICGCFQGPQTDEGVQLQIIKALLTAVTSQHIEIHEGTVLQAVRTCYNIYLASKNLINQTTAKATLTQMLNVIFARMENQALQEAKQMEKERHRQHHHLLQSPVSHHEPESPQLRYLPPQTVDHIPQEHEGDLDPQTNDVDKSLQDDTEPENGSDISSAENEQTEADQATAAETLSKNDILYDGENHDCEEKPQDIVQSIVEEMVNIVVGDTGERTTINVSADGNNGTIEDGSDSENIQANGIPGTPISVAYTPSLPDDRLSVSSNDTQESGNSSGPSPGAKFSHILQKDAFLVFRSLCKLSMKPLSDGPPDPKSHELRSKILSLQLLLSILQNAGPIFGTNEMFINAIKQYLCVALSKNGVSSVPEVFELSLSIFLTLLSNFKTHLKMQIEVFFKEIFLYILETSTSSFDHKWMVIQTLTRICADAQSVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRGSQELGMSNVQELSLRKKGLECLVSILKCMVEWSKDQYVNPNSQTTLGQEKPSEQETSEMKHPETINRYGSLNSLESTSSSGIGSYSTQMSGTDNPEQFEVLKQQKEIIEQGIDLFTKKPKRGIQYLQEQGMLGTTPEDIAQFLHQEERLDSTQVGEFLGDNDKFNKEVMYAYVDQHDFSGKDFVSALRMFLEGFRLPGEAQKIDRLMEKFAARYLECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSAIYNEIAGKKISMKETKELTIPAKSSKQNVASEKQRRLLYNLEMEQMAKTAKALMEAVSHVQAPFTSATHLEHVRPMFKLAWTPFLAAFSVGLQDCDDTEVASLCLEGIRCAIRIACIFSIQLERDAYVQALARFTLLTVSSGITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKPRYISGTVRGREGSLTGAKDQAPDEFVGLGLVGGNVDWKQIASIQESIGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELLSTTHPRMFSLQKIVEISYYNMGRIRLQWSRIWEVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKRNRSPTIRDMVVRCIAQMVNSQAANIRSGWKNIFSVFHLAASDQDESIVELAFQTTGHIVTLVFEKHFPATIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRHCAKYVSDRPQAFKEYTSDDMNVAPEDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKTYGYTYEKHWWQDLFRIVFRIFDNMKLPEQQTEKAEWMTTTCNHALYAICDVFTQYLEVLSDVLLDDIFAQLYWCVQQDNEQLARSGTNCLENVVILNGEKFTLEIWDKTCNCTLDIFKTTIPHALLTWRPISGETAPPTPSPVSENQLDTISQKSVDIHDSIQPRSADNRQQAPLASVSTVNEEISKIKPTAKFPEQKLFAALLIKCVVQLELIQTIDNIVFFPATSRKEDAENLAAAQRDAVDFDVRVDTQDQGMYRFLTSQQLFKLLDCLLESHRFAKAFNSNNEQRTALWKAGFKGKSKPNLLKQETSSLACGLRILFRMYTDESRASAWEEVQQRLLNVCSEALSYFLTLTSESHREAWTNLLLLFLTKVLKISDNRFKAHASFYYPLLCEIMQFDLIPELRAVLRRFFLRIGVVFQISQPPEQELGINKQ

Target ORF information:

RefSeq Version	NM_173997.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ADP ribosylation factor guanine nucleotide exchange factor 1 (ARFGEF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173997.2

ORF Insert Sequence:

ATGTATGAGG GGAAGAAGAC GAAGAACATG TTCCTGACCC GGGCCCTGGA GAAGATTTTG 
GCCGACAAGG AAGTGAAGAA GGCGCATCAC TCCCAGCTGC GCAAAGCGTG CGAGGTGGCG 
TTAGAGGAAA TAAAAGCGGA AACTGAAAAG CAGAGTCCTC CTCACGGAGA AGCAAAAGCT 
GGATCAAGCA CCCTTCCACC AGTGAAATCA AAGACAAATT TTATTGAAGC AGACAAGTAC 
TTCCTCCCCT TTGAATTGGC ATGCCAGTCC AAATGTCCCC GCATAGTCAG TACATCTCTA 
GATTGTTTAC AGAAACTTAT TGCTTATGGG CACTTGACTG GCAATGCTCC AGATAGTACA 
ACACCAGGCA AAAAATTAAT TGATAGAATT ATTGAAACAA TTTGTGGCTG TTTCCAAGGT 
CCTCAAACAG ATGAAGGAGT TCAGTTACAA ATAATAAAGG CTTTACTAAC TGCAGTAACA 
TCTCAACACA TAGAAATTCA TGAAGGAACT GTACTGCAAG CTGTGAGAAC ATGTTACAAT 
ATATATCTAG CAAGCAAAAA TCTCATCAAT CAGACAACAG CCAAAGCTAC TCTTACTCAG 
ATGTTAAATG TTATCTTTGC ACGCATGGAA AACCAAGCAT TGCAGGAAGC CAAACAAATG 
GAAAAAGAAA GGCATCGACA GCACCACCAT CTGTTGCAGT CTCCAGTAAG CCATCATGAG 
CCTGAATCAC CTCAACTCAG ATACTTGCCA CCTCAGACTG TTGATCACAT ACCCCAAGAA 
CATGAAGGGG ACCTTGATCC CCAGACAAAT GATGTGGATA AAAGCCTTCA GGATGATACA 
GAGCCCGAAA ATGGATCTGA TATTTCCAGT GCAGAAAATG AACAGACTGA AGCCGATCAG 
GCAACTGCAG CCGAAACATT GTCTAAAAAT GATATATTAT ATGATGGAGA AAACCATGAC 
TGTGAGGAAA AGCCACAGGA CATTGTACAG AGCATTGTAG AAGAAATGGT GAACATCGTT 
GTTGGAGATA CGGGAGAAAG GACTACCATA AATGTAAGTG CAGATGGCAA CAATGGAACC 
ATAGAGGATG GTAGCGACAG TGAAAATATT CAAGCAAACG GAATTCCAGG AACACCAATT 
TCTGTTGCTT ATACACCGTC CTTACCTGAT GACAGATTGT CTGTCTCTTC CAATGATACT 
CAGGAATCTG GAAATTCTTC AGGACCTTCA CCTGGTGCTA AGTTTTCCCA CATTTTACAA 
AAGGATGCCT TTCTAGTATT CAGGTCATTG TGTAAACTGT CAATGAAACC ACTATCGGAT 
GGACCACCGG ATCCAAAGTC TCATGAGTTA CGATCCAAGA TTCTTTCACT GCAGTTACTT 
CTATCCATTC TGCAGAATGC AGGACCTATT TTCGGGACAA ATGAGATGTT TATTAATGCT 
ATAAAGCAGT ATCTGTGTGT TGCACTCTCA AAAAATGGAG TTTCATCTGT CCCAGAGGTT 
TTTGAGCTGT CTCTTTCCAT ATTTCTTACT TTGTTATCAA ACTTCAAAAC ACATCTGAAG 
ATGCAGATTG AGGTGTTCTT TAAAGAAATT TTCCTATATA TTTTGGAGAC CTCTACCAGC 
TCATTTGACC ACAAATGGAT GGTTATTCAG ACATTAACAA GGATTTGTGC AGATGCTCAG 
AGTGTAGTGG ATATATATGT AAACTATGAT TGTGACTTAA ATGCAGCAAA CATATTTGAA 
CGACTAGTAA ATGATCTGTC AAAAATTGCC CAAGGACGAG GCAGTCAGGA ACTTGGTATG 
AGTAATGTTC AGGAACTGAG CCTAAGGAAA AAAGGTTTAG AATGCTTAGT GTCAATTTTG 
AAGTGCATGG TTGAATGGAG CAAGGATCAG TATGTGAATC CCAACTCACA GACGACTCTT 
GGTCAGGAAA AACCCTCAGA GCAAGAGACG AGTGAAATGA AACACCCAGA GACAATAAAC 
AGATACGGAA GTTTAAATTC CCTGGAGTCA ACGTCATCAT CAGGAATAGG CAGCTACAGC 
ACACAGATGT CTGGCACTGA TAATCCGGAG CAGTTCGAAG TCCTCAAGCA GCAGAAAGAA 
ATAATAGAAC AAGGGATAGA CTTATTTACT AAGAAACCAA AGAGAGGAAT CCAGTACCTC 
CAAGAACAAG GGATGCTTGG CACCACCCCT GAAGACATTG CCCAGTTCTT ACACCAAGAG 
GAAAGATTAG ACTCAACTCA AGTGGGTGAA TTCCTGGGAG ATAATGATAA ATTTAACAAA 
GAAGTCATGT ATGCTTATGT GGACCAACAT GACTTTTCAG GAAAAGATTT TGTTTCAGCC 
CTTCGTATGT TTCTGGAAGG ATTCCGTCTT CCAGGGGAAG CTCAGAAAAT TGATCGATTA 
ATGGAAAAAT TTGCTGCAAG ATATCTAGAA TGCAACCAAG GGCAAACCCT TTTTGCCAGT 
GCAGATACTG CTTATGTTTT GGCTTATTCA ATTATCATGC TGACCACAGA TCTTCATAGT 
CCACAGGTTA AAAATAAAAT GACCAAGGAA CAGTACATTA AGATGAATAG AGGCATCAAT 
GACAGTAAAG ATCTCCCTGA AGAGTATCTG TCAGCCATCT ATAATGAAAT AGCTGGAAAA 
AAGATATCAA TGAAAGAAAC GAAGGAGCTG ACAATCCCTG CAAAGTCAAG TAAACAGAAC 
GTAGCCAGTG AAAAACAAAG AAGACTCCTG TATAACTTAG AGATGGAACA GATGGCCAAG 
ACCGCTAAAG CTCTCATGGA GGCTGTGAGC CACGTTCAGG CACCTTTCAC GAGTGCAACA 
CATTTGGAGC ATGTGAGACC CATGTTTAAG CTGGCTTGGA CCCCTTTTCT GGCTGCATTC 
AGTGTGGGTC TACAAGACTG TGATGATACT GAAGTAGCCT CTCTTTGCCT GGAAGGTATA 
AGATGTGCAA TCAGAATTGC ATGCATTTTC AGCATTCAGC TGGAAAGAGA TGCATACGTC 
CAGGCGCTGG CAAGATTCAC CTTACTCACA GTGAGTTCTG GGATTACTGA AATGAAGCAG 
AAGAACATTG ACACAATCAA AACGCTTATC ACAGTGGCTC ATACAGATGG AAATTACTTA 
GGAAATTCAT GGCATGAGAT TCTGAAGTGC ATCAGTCAGC TGGAGCTTGC TCAACTTATA 
GGAACTGGAG TGAAGCCTCG GTACATTTCT GGAACAGTAC GAGGCAGAGA AGGATCACTT 
ACTGGAGCAA AAGATCAGGC TCCTGATGAA TTTGTGGGGC TAGGGCTAGT TGGAGGAAAT 
GTGGACTGGA AGCAGATAGC AAGTATTCAG GAATCCATTG GAGAAACCAG TTCTCAAAGT 
GTTGTTGTTG CTGTAGATAG AATATTTACA GGGTCCACAA GGCTAGATGG AAATGCCATT 
GTGGATTTTG TCCGTTGGCT CTGTGCTGTG TCTATGGATG AATTACTTTC TACTACGCAT 
CCAAGAATGT TTAGTCTGCA AAAAATAGTA GAAATATCAT ATTACAACAT GGGAAGAATA 
AGATTGCAGT GGTCTCGAAT TTGGGAAGTT ATTGGAGATC ATTTTAATAA GGTTGGCTGT 
AATCCTAATG AAGATGTAGC TATTTTCGCA GTAGACTCCC TGAGGCAGCT GTCAATGAAG 
TTCTTAGAGA AAGGGGAGCT TGCTAATTTC AGATTCCAGA AGGATTTCTT AAGACCTTTT 
GAACATATAA TGAAACGGAA CAGGTCTCCA ACAATTCGCG ATATGGTTGT ACGGTGTATA 
GCGCAGATGG TTAACTCTCA AGCTGCTAAC ATTCGGTCCG GATGGAAAAA CATCTTCTCT 
GTATTTCATC TTGCTGCATC GGATCAAGAT GAAAGCATAG TTGAGCTTGC ATTCCAGACA 
ACAGGACATA TTGTCACCCT TGTGTTTGAA AAACACTTTC CAGCGACCAT TGATTCTTTC 
CAGGATGCAG TGAAATGTTT GTCTGAATTT GCGTGCAATG CAGCTTTCCC AGATACAAGT 
ATGGAAGCAA TTCGACTTAT TCGCCATTGT GCAAAATACG TGTCTGATAG ACCTCAGGCT 
TTCAAGGAGT ACACAAGTGA TGACATGAAT GTGGCACCTG AAGACAGAGT GTGGGTGAGA 
GGCTGGTTCC CAATTCTCTT CGAGTTATCA TGTATCATCA ATAGATGCAA ATTAGATGTA 
AGAACCAGGG GTTTAACAGT AATGTTTGAA ATAATGAAAA CTTACGGCTA CACTTATGAA 
AAACACTGGT GGCAGGATTT ATTTAGAATT GTTTTCAGAA TCTTTGACAA TATGAAATTA 
CCAGAACAGC AGACAGAGAA AGCCGAATGG ATGACGACAA CATGCAATCA TGCACTCTAT 
GCGATCTGTG ACGTGTTCAC CCAGTACTTA GAAGTGCTCA GTGATGTGCT TTTGGACGAC 
ATTTTTGCCC AGCTCTACTG GTGTGTGCAG CAAGACAATG AGCAGTTGGC ACGATCTGGT 
ACAAACTGCT TAGAGAATGT TGTTATTCTG AATGGTGAAA AATTCACACT AGAAATCTGG 
GATAAAACGT GCAACTGCAC ACTGGACATC TTCAAAACCA CAATCCCACA TGCGCTCTTG 
ACCTGGCGTC CCATTTCTGG AGAAACTGCT CCCCCAACCC CATCTCCTGT GAGTGAAAAC 
CAGTTGGATA CAATATCACA GAAATCTGTA GATATTCATG ATTCTATTCA ACCAAGATCT 
GCAGATAATA GACAGCAAGC TCCGTTGGCT TCTGTCTCTA CTGTGAATGA AGAAATCAGC 
AAAATTAAAC CCACAGCAAA ATTTCCAGAA CAAAAATTGT TTGCCGCCCT GTTGATTAAA 
TGTGTTGTGC AGCTGGAACT CATCCAGACT ATCGACAACA TCGTGTTCTT CCCCGCCACA 
AGTAGGAAGG AGGACGCGGA AAACTTAGCC GCGGCGCAGA GAGACGCTGT GGACTTTGAT 
GTTCGAGTGG ACACTCAAGA CCAAGGAATG TATCGCTTTT TAACATCACA ACAACTCTTC 
AAGCTCCTGG ACTGCTTGTT AGAGTCACAC AGATTTGCAA AAGCTTTCAA CTCCAACAAC 
GAACAGAGGA CGGCTCTGTG GAAGGCGGGC TTCAAGGGCA AATCCAAGCC CAACCTTCTG 
AAGCAGGAGA CGAGCAGCCT GGCCTGCGGG CTGCGCATTC TCTTCCGGAT GTACACGGAC 
GAGAGCCGGG CCAGCGCCTG GGAGGAGGTC CAGCAGAGGC TTTTAAATGT CTGCAGCGAA 
GCACTCAGTT ACTTTCTTAC TCTAACGTCA GAAAGTCATC GGGAAGCCTG GACTAACTTA 
CTGCTCTTAT TTCTGACTAA AGTCCTGAAG ATCAGCGATA ATAGGTTTAA AGCTCATGCA 
TCATTCTACT ACCCTCTCTT ATGTGAAATT ATGCAATTTG ACTTGATTCC TGAACTTCGT 
GCTGTTCTTA GAAGATTTTT TCTGAGAATT GGAGTAGTTT TTCAGATATC ACAACCACCT 
GAACAGGAAC TTGGAATAAA CAAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning and expression of a cDNA encoding a bovine brain brefeldin A-sensitive guanine nucleotide-exchange protein for ADP-ribosylation factor.
Proceedings of the National Academy of Sciences of the United States of America94(24)12926-31(1997 Nov)
Morinaga N,Moss J,Vaughan M

Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain.
Proceedings of the National Academy of Sciences of the United States of America93(23)12856-60(1996 Nov)
Morinaga N,Tsai SC,Moss J,Vaughan M

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ARFGEF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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