SPEF2 gene cDNA ORF clone, Pan troglodytes(chimpanzee)

mRNA	Protein	Name
XM_009449293.2	XP_009447568.2	sperm flagellar protein 2 isoform X11
XM_016953431.1	XP_016808920.1	sperm flagellar protein 2 isoform X3
XM_016953436.1	XP_016808925.1	sperm flagellar protein 2 isoform X7
XM_024356634.1	XP_024212402.1	sperm flagellar protein 2 isoform X2
XM_016953432.2	XP_016808921.2	sperm flagellar protein 2 isoform X4
XM_016953431.2	XP_016808920.2	sperm flagellar protein 2 isoform X7
XM_009449293.3	XP_009447568.3	sperm flagellar protein 2 isoform X14
XM_016953430.1	XP_016808919.1	sperm flagellar protein 2 isoform X1
XM_009449294.2	XP_009447569.2	sperm flagellar protein 2 isoform X2
XM_016953435.1	XP_016808924.1	sperm flagellar protein 2 isoform X6
XM_016953437.1	XP_016808926.1	sperm flagellar protein 2 isoform X8
XM_016953430.2	XP_016808919.2	sperm flagellar protein 2 isoform X5
XM_009449294.3	XP_009447569.3	sperm flagellar protein 2 isoform X6
XM_016953432.1	XP_016808921.1	sperm flagellar protein 2 isoform X4
XM_016953438.1	XP_016808927.1	sperm flagellar protein 2 isoform X9
XM_024356637.1	XP_024212405.1	sperm flagellar protein 2 isoform X9
XM_016953436.2	XP_016808925.2	sperm flagellar protein 2 isoform X10
XM_016953437.2	XP_016808926.2	sperm flagellar protein 2 isoform X11
XM_016953438.2	XP_016808927.2	sperm flagellar protein 2 isoform X12
XM_016953433.1	XP_016808922.1	sperm flagellar protein 2 isoform X5
XM_016953439.1	XP_016808928.1	sperm flagellar protein 2 isoform X10
XM_024356633.1	XP_024212401.1	sperm flagellar protein 2 isoform X1
XM_024356635.1	XP_024212403.1	sperm flagellar protein 2 isoform X3
XM_024356636.1	XP_024212404.1	sperm flagellar protein 2 isoform X8
XM_016953439.2	XP_016808928.2	sperm flagellar protein 2 isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc106455	XM_009449293.3 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OPc85080	XM_009449293.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPc106442	XM_024356633.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106443	XM_024356634.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106444	XM_024356635.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106445	XM_016953432.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106446	XM_016953430.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106447	XM_009449294.3 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106448	XM_016953431.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106449	XM_024356636.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106450	XM_024356637.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc106451	XM_016953436.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc106452	XM_016953437.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc106453	XM_016953438.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc106454	XM_016953439.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OPc85070	XM_016953430.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc85071	XM_009449294.2 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc85072	XM_016953431.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc85073	XM_016953432.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc85074	XM_016953433.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc85075	XM_016953435.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc85076	XM_016953436.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc85077	XM_016953437.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc85078	XM_016953438.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc85079	XM_016953439.1 Latest version!	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

SPEF2 ( XM_009449293.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009447568.3 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPc106455
Clone ID Related Accession (Same CDS sequence)	XM_009449293.3
Accession Version	XM_009449293.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009449293.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCAGT CCCAGCAGGA GCGCAGGATT GCCGTGCAGC TCATGCATGT TCGGCATGAA 
AAGGAAGTTT TATGGCAAAA CAGAATTTTC AGAGAAAAAC AACATGAGGA AAGACGACTT 
AAAGATTTCC AGGATGCTCT TGATCGAGAA GCGGCTTTGG CAAAACAAGC CAAGATTGAC 
TTTGAAGAAC AATTCCTTAA AGAAAAGAGA TTTCATGATC AGATTGCTGT GGAAAGAGCT 
CAAGCTCGTT ATGAAAAGCA TTATTCAGTA TGTGCAGAAA TTTTGGATCA AATAGTTGAT 
TTGTCCACTA AAGTGGCAGA CTATCGAATG TTGACAAATA ATCTGATTCC GTATAAGTTG 
ATGCATGATT GGAAGGAACT ATTTTTTAAT GCAAAACCCA TATATGAACA AGCCTCTGTT 
AAGACACTAC CTGCTGACCC CTCAAGAGAA CAACTTACAG AACTGGAGAA AAGGGACTTG 
CTAGATACCA ATGATTATGA AGAATATAAG ATATGGAGGA ATACAGCTGA CCTCTCAGTG 
GACAGAAACA TGGTTGGAGA GTGGGCCTTA CCAGAAGAAA TGGTTGACAA TTTACCACCC 
TCCAACAATT GCATACTGGG CCATATTCTT CACAGGCTAG TTGAAAAATC TCTTCCTCCT 
CGAGCGGAAT CAACAACACC TGAATTACCT TCATTTGCTG TTAAAGGATG CTTATTGGGG 
AAAACATTAA GTGGAAAAAC TACCATTTTA AGGTCTCTAC AAAAAGACTT TCCTATACAG 
ATACTTTCTA TTGACACTCT TGTCCAAGAA GCTATCCAAG CATTTCATGA CAATGAAAAA 
GTCAGTGAGG TTCTACCAAT TCAGAAAAAA GATGAAGAAG ATGCTCTACC AGTTCTGCAA 
GAGGAGATTA AAGAAAGCCA GGATCCACAA CATGTATTTT CAGCTGGTCC AGTTTCAGAT 
GAAGTATTAC CAGAAACAGA AGGTGAAACA ATACTTAGTG GTAATGCTGA TAAAACACCA 
AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTTAAAAC TCACTACACG TGCTCAGCTT 
GGTGCAAAAT CAGAACAGTT GCTGAAGAAA GGAAAGAGCA TTCCTGATGT GCTGCTTGTT 
GACATCATGG TAAATGCTAT TAACAAGATA CCTGTGAATC AAGACTGTAT CCTAGATGGT 
TTTCCAATGA CTTTAAACCA AGCACAGCTT CTGGAAGAAG CTCTTACAGG CTGCAATAGA 
AACCTCACAG AAGTGGAAAG AAAAAAAGCA CAAAAATCCA CATTGGCTAT TGATCCTGCG 
ACTTCCAAAG AAATACCTCT TCCCTCTCCT GCATTTGATT TTGTCATATT ATTAGATGTT 
TCAGATACTT CCTCAATGAG TCGCATGAAT GATATTATAG CTGAAGAATT GTCCTATAAA 
ACTGCTCACG AAGATATCAG TCAACGTGTA GCTGCTGAAA ACCAAGATAA GGATGGAGAC 
CAAAATTTAA GAGACCAGAT ACAACATAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTA 
TTGGAGCAAT GGTTTTCAGA GCCAGAAAAT ATTTTGATAA AAATCAATGC TGAAATAGAT 
AAGGAATCTT TATGTGAAAA AGTAAAAGAA ATTCTTACGA CTGAAATAGC AAAAAAAAAG 
AATAAAGTTG AGAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGCAGC AGCTTCCCTG 
GCTGAGCTTC CACTTCCTAC ACCTCCTCCT GCTCCTCCTC CTGAACCAGA AAAAGAGAAG 
GAAATTCATC AAAGCCATGT GCCTTCAAAA ACTCCTACTG CAAAAGGAAA ACCTCAATCA 
GAAGCCCCGC ATGGTAAGCA AGAATCTCTT CAGGAAGGAA AAGGGAAGAA AGGTGAGACC 
GCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCATCAG GAGGAAAAGT ACCAGTAAAG 
AAATCACCTG CTGACTCTAC AGATACATCA CCTGTTGCAA TAGTGCCACA GCCACCTAAG 
CCAGGATCAG AAGAATGGGT CTATGTGAAT GAACCAGTTC CTGAGGAAAT GCCTTCGTTT 
TTAGTACCTT ACTGGGAACT AATAGAAAAT TCCTATATAA ACACCATCAA AACAGTACTC 
AGGCATCTGA GGGAAGATCA GCATACTGTG CTTGCTTACT TATATGAGAT TAGAACAAGT 
TTCCAGGAGT TTCTAAAGCG TCCAGATCAC AAGCAAGATT TTGTAGCTCA ATGGCAGGCT 
GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAACTACAT 
CAACGAGTGA ATGATCTGCG AGACCGCCTG TGGGACATTT GTGATGTCCG GAAGGAAGAG 
GCAGAGCAGG AGCGGCTTGA CATCATTAAT GAGAGCTGGT TACAGGACAC TCTTGGAATG 
ACAATGAACC ATTTCTTTTC CCTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACAAAG 
AGACTCCTTC AAGATTATTA CTGGGGAATG GAAAGTAAAA TCCCAGTAGA GGACAACAAG 
AGATTTACTC AAATCCCTTT GGTCCAACTG GATAGTAAAG ACAATTCCGA AAGCCAGCTT 
AGAATTCCTC TAGTTCCTCG AATATCCATT TCTCTGGAAA CAGTTACACC CAAACCAAAA 
ACAAAATCAG TACTGAAGAG CAAGACGGAT AACTCCCTAG AAAACGTTGA GTCCAACTTT 
GAGGCCGATG AAAAGTTGGT CATGGACACC TGGCAGCAGG CTTCTTTAGC AGTATCTCAC 
ATGGTGGCTG CTGAAATTCA TCAGAGGCTT ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA 
GACCCCAAAG AAAAATCTCC TCAGATGGGT GCAAATAAAA AAGTCAAAAA GGAGCCACCC 
AAGAAAAAAC AGGAAGACAA AAAACCCAAA GGTAAATCAT CACCTATGGC AGAAGCAACT 
CCTGTCATAG TAACAACAGA GGAAATTGCT GAAATCAAAA GGAAAAATGA ACTGAGGGTC 
AAAATAAAAG AAGAACACCT TGCTGCCTTG CAATTTGAAG AAATAGCCAC GCAATTTCGA 
CTTGAACTGA TAAAGACAAA AGCATTGGCT CTTCTTGAAG ATTTAGTAAC AAAGGTGGTT 
GATGTATATA AACTCATGGA AAAATGGCTT GGTGAGAGGT ATTTGAATGA AATGGCCAGT 
ATAGAAAAAT TAACTGACGT AGCTCGCTAT CACATTGAAA CATCTACAAA AATTCAGAAT 
GAACTTTATT TAAGCCAAGA AGACTTCTTC ATTAATGGCA ATATAAAAGT CTTCCCAGAT 
CCTCCCCCAC CAATACGTCC TCCACCTGTA GAAAAGGAAG AAGATGGCAC CCTGACCATT 
GAACAGCTTG ACAGTCTTCG AGATCAGTTC TTAGATATGG CACCTAACGG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA GTTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCGTTCCCT CGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTCGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATA AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGCCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GGAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA GGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAACAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009447568.3
CDS	2134..6627
Translation	MRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_009449293.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009449293.3

ORF Insert Sequence:

ATGCGGCAGT CCCAGCAGGA GCGCAGGATT GCCGTGCAGC TCATGCATGT TCGGCATGAA 
AAGGAAGTTT TATGGCAAAA CAGAATTTTC AGAGAAAAAC AACATGAGGA AAGACGACTT 
AAAGATTTCC AGGATGCTCT TGATCGAGAA GCGGCTTTGG CAAAACAAGC CAAGATTGAC 
TTTGAAGAAC AATTCCTTAA AGAAAAGAGA TTTCATGATC AGATTGCTGT GGAAAGAGCT 
CAAGCTCGTT ATGAAAAGCA TTATTCAGTA TGTGCAGAAA TTTTGGATCA AATAGTTGAT 
TTGTCCACTA AAGTGGCAGA CTATCGAATG TTGACAAATA ATCTGATTCC GTATAAGTTG 
ATGCATGATT GGAAGGAACT ATTTTTTAAT GCAAAACCCA TATATGAACA AGCCTCTGTT 
AAGACACTAC CTGCTGACCC CTCAAGAGAA CAACTTACAG AACTGGAGAA AAGGGACTTG 
CTAGATACCA ATGATTATGA AGAATATAAG ATATGGAGGA ATACAGCTGA CCTCTCAGTG 
GACAGAAACA TGGTTGGAGA GTGGGCCTTA CCAGAAGAAA TGGTTGACAA TTTACCACCC 
TCCAACAATT GCATACTGGG CCATATTCTT CACAGGCTAG TTGAAAAATC TCTTCCTCCT 
CGAGCGGAAT CAACAACACC TGAATTACCT TCATTTGCTG TTAAAGGATG CTTATTGGGG 
AAAACATTAA GTGGAAAAAC TACCATTTTA AGGTCTCTAC AAAAAGACTT TCCTATACAG 
ATACTTTCTA TTGACACTCT TGTCCAAGAA GCTATCCAAG CATTTCATGA CAATGAAAAA 
GTCAGTGAGG TTCTACCAAT TCAGAAAAAA GATGAAGAAG ATGCTCTACC AGTTCTGCAA 
GAGGAGATTA AAGAAAGCCA GGATCCACAA CATGTATTTT CAGCTGGTCC AGTTTCAGAT 
GAAGTATTAC CAGAAACAGA AGGTGAAACA ATACTTAGTG GTAATGCTGA TAAAACACCA 
AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTTAAAAC TCACTACACG TGCTCAGCTT 
GGTGCAAAAT CAGAACAGTT GCTGAAGAAA GGAAAGAGCA TTCCTGATGT GCTGCTTGTT 
GACATCATGG TAAATGCTAT TAACAAGATA CCTGTGAATC AAGACTGTAT CCTAGATGGT 
TTTCCAATGA CTTTAAACCA AGCACAGCTT CTGGAAGAAG CTCTTACAGG CTGCAATAGA 
AACCTCACAG AAGTGGAAAG AAAAAAAGCA CAAAAATCCA CATTGGCTAT TGATCCTGCG 
ACTTCCAAAG AAATACCTCT TCCCTCTCCT GCATTTGATT TTGTCATATT ATTAGATGTT 
TCAGATACTT CCTCAATGAG TCGCATGAAT GATATTATAG CTGAAGAATT GTCCTATAAA 
ACTGCTCACG AAGATATCAG TCAACGTGTA GCTGCTGAAA ACCAAGATAA GGATGGAGAC 
CAAAATTTAA GAGACCAGAT ACAACATAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTA 
TTGGAGCAAT GGTTTTCAGA GCCAGAAAAT ATTTTGATAA AAATCAATGC TGAAATAGAT 
AAGGAATCTT TATGTGAAAA AGTAAAAGAA ATTCTTACGA CTGAAATAGC AAAAAAAAAG 
AATAAAGTTG AGAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGCAGC AGCTTCCCTG 
GCTGAGCTTC CACTTCCTAC ACCTCCTCCT GCTCCTCCTC CTGAACCAGA AAAAGAGAAG 
GAAATTCATC AAAGCCATGT GCCTTCAAAA ACTCCTACTG CAAAAGGAAA ACCTCAATCA 
GAAGCCCCGC ATGGTAAGCA AGAATCTCTT CAGGAAGGAA AAGGGAAGAA AGGTGAGACC 
GCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCATCAG GAGGAAAAGT ACCAGTAAAG 
AAATCACCTG CTGACTCTAC AGATACATCA CCTGTTGCAA TAGTGCCACA GCCACCTAAG 
CCAGGATCAG AAGAATGGGT CTATGTGAAT GAACCAGTTC CTGAGGAAAT GCCTTCGTTT 
TTAGTACCTT ACTGGGAACT AATAGAAAAT TCCTATATAA ACACCATCAA AACAGTACTC 
AGGCATCTGA GGGAAGATCA GCATACTGTG CTTGCTTACT TATATGAGAT TAGAACAAGT 
TTCCAGGAGT TTCTAAAGCG TCCAGATCAC AAGCAAGATT TTGTAGCTCA ATGGCAGGCT 
GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAACTACAT 
CAACGAGTGA ATGATCTGCG AGACCGCCTG TGGGACATTT GTGATGTCCG GAAGGAAGAG 
GCAGAGCAGG AGCGGCTTGA CATCATTAAT GAGAGCTGGT TACAGGACAC TCTTGGAATG 
ACAATGAACC ATTTCTTTTC CCTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACAAAG 
AGACTCCTTC AAGATTATTA CTGGGGAATG GAAAGTAAAA TCCCAGTAGA GGACAACAAG 
AGATTTACTC AAATCCCTTT GGTCCAACTG GATAGTAAAG ACAATTCCGA AAGCCAGCTT 
AGAATTCCTC TAGTTCCTCG AATATCCATT TCTCTGGAAA CAGTTACACC CAAACCAAAA 
ACAAAATCAG TACTGAAGAG CAAGACGGAT AACTCCCTAG AAAACGTTGA GTCCAACTTT 
GAGGCCGATG AAAAGTTGGT CATGGACACC TGGCAGCAGG CTTCTTTAGC AGTATCTCAC 
ATGGTGGCTG CTGAAATTCA TCAGAGGCTT ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA 
GACCCCAAAG AAAAATCTCC TCAGATGGGT GCAAATAAAA AAGTCAAAAA GGAGCCACCC 
AAGAAAAAAC AGGAAGACAA AAAACCCAAA GGTAAATCAT CACCTATGGC AGAAGCAACT 
CCTGTCATAG TAACAACAGA GGAAATTGCT GAAATCAAAA GGAAAAATGA ACTGAGGGTC 
AAAATAAAAG AAGAACACCT TGCTGCCTTG CAATTTGAAG AAATAGCCAC GCAATTTCGA 
CTTGAACTGA TAAAGACAAA AGCATTGGCT CTTCTTGAAG ATTTAGTAAC AAAGGTGGTT 
GATGTATATA AACTCATGGA AAAATGGCTT GGTGAGAGGT ATTTGAATGA AATGGCCAGT 
ATAGAAAAAT TAACTGACGT AGCTCGCTAT CACATTGAAA CATCTACAAA AATTCAGAAT 
GAACTTTATT TAAGCCAAGA AGACTTCTTC ATTAATGGCA ATATAAAAGT CTTCCCAGAT 
CCTCCCCCAC CAATACGTCC TCCACCTGTA GAAAAGGAAG AAGATGGCAC CCTGACCATT 
GAACAGCTTG ACAGTCTTCG AGATCAGTTC TTAGATATGG CACCTAACGG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA GTTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCGTTCCCT CGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTCGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATA AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGCCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GGAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA GGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAACAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_009449293.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009447568.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OPc85080
Clone ID Related Accession (Same CDS sequence)	XM_009449293.2
Accession Version	XM_009449293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009449293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCAGT CCCAGCAGGA GCGCAGGATT GCCGTGCAGC TCATGCATGT TCGGCATGAA 
AAGGAAGTTT TATGGCAAAA CAGAATTTTC AGAGAAAAAC AACATGAGGA AAGACGACTT 
AAAGATTTCC AGGATGCTCT TGATCGAGAA GCGGCTTTGG CAAAACAAGC CAAGATTGAC 
TTTGAAGAAC AATTCCTTAA AGAAAAGAGA TTTCATGATC AGATTGCTGT GGAAAGAGCT 
CAAGCTCGTT ATGAAAAGCA TTATTCAGTA TGTGCAGAAA TTTTGGATCA AATAGTTGAT 
TTGTCCACTA AAGTGGCAGA CTATCGAATG TTGACAAATA ATCTGATTCC GTATAAGTTG 
ATGCATGATT GGAAGGAACT ATTTTTTAAT GCAAAACCCA TATATGAACA AGCCTCTGTT 
AAGACACTAC CTGCTGACCC CTCAAGAGAA CAACTTACAG AACTGGAGAA AAGGGACTTG 
CTAGATACCA ATGATTATGA AGAATATAAG AACATGGTTG GAGAGTGGGC CTTACCAGAA 
GAAATGGTTG ACAATTTACC ACCCTCCAAC AATTGCATAC TGGGCCATAT TCTTCACAGG 
CTAGTTGAAA AATCTCTTCC TCCTCGAGCG GAATCAACAA CACCTGAATT ACCTTCATTT 
GCTGTTAAAG GATGCTTATT GGGGAAAACA TTAAGTGGAA AAACTACCAT TTTAAGGTCT 
CTACAAAAAG ACTTTCCTAT ACAGATACTT TCTATTGACA CTCTTGTCCA AGAAGCTATC 
CAAGCATTTC ATGACAATGA AAAAGTCAGT GAGGTTCTAC CAATTCAGAA AAAAGATGAA 
GAAGATGCTC TACCAGTTCT GCAAGAGGAG ATTAAAGAAA GCCAGGATCC ACAACATGTA 
TTTTCAGCTG GTCCAGTTTC AGATGAAGTA TTACCAGAAA CAGAAGGTGA AACAATACTT 
AGTGGTAATG CTGATAAAAC ACCAAAAGCT GAAGAAGTCA AATCAAGTGA TAGTTTCTTA 
AAACTCACTA CACGTGCTCA GCTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ATGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACAA GATACCTGTG 
AATCAAGACT GTATCCTAGA TGGTTTTCCA ATGACTTTAA ACCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTGCAA TAGAAACCTC ACAGAAGTGG AAAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAATAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCA TATTATTAGA TGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAATGATATT 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGATA TCAGTCAACG TGTAGCTGCT 
GAAAACCAAG ATAAGGATGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGGAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGTG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGCAAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCAAAGCC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA GACCGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGATAC ATCACCTGTT 
GCAATAGTGC CACAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ATCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AACTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACATCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGATG TCCGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATAAGAGC 
TGGTTACAGG ACACTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC AAAGAGACTC CTTCAAGATT ATTACTGGGG AATGGAAAGT 
AAAATCCCAG TAGAGGACAA CAAGAGATTT ACTCAAATCC CTTTGGTCCA ACTGGATAGT 
AAAGACAATT CCGAAAGCCA GCTTAGAATT CCTCTAGTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CACCCAAACC AAAAACAAAA TCAGTACTGA AGAGCAAGAC GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCC GATGAAAAGT TGGTCATGGA CACCTGGCAG 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCTGAAA TTCATCAGAG GCTTATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACC CAAAGGTAAA 
TCATCACCTA TGGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
AAAAGGAAAA ATGAACTGAG GGTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAATAG CCACGCAATT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGGTATTTGA ATGAAATGGC CAGTATAGAA AAATTAACTG ACGTAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAGCC AAGAAGACTT CTTCATTAAT 
GGCAATATAA AAGTCTTCCC AGATCCTCCC CCACCAATAC GTCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATTGAACAG CTTGACAGTC TTCGAGATCA GTTCTTAGAT 
ATGGCACCTA ACGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCCGAGT TCGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCGTT CCCTCGGAGG AGGAGCTCCT TGAGACCCTT 
CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGAAAA TCCTCTTGAA 
CCCCTTCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATAAGAAGG ATCCACCCCA GCTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGC CTACAGGGCC CTCAGTGTGG CTGTTGGAAC TCATGTCTTC 
CAACAAGTCA AAGCTTCCAT TCCAAGTGCA GAAAAGACCT CCTCAACTGA TGCAGGTCCA 
GCTGAGGAAT TTCCTGAACC TGAGGGAAAT GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC 
GACAGGGAGA AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATGTCCATGG AAACACTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCCAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTCA CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGTACA CATCATCTAT 
GTAGTGGAAA TGATAGTGTG TGCAAAAGGT TGGGCACCAC TTCCATCAGG AAAACAGTGT 
GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009447568.2
CDS	2122..6579
Translation	MRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_009449293.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009449293.2

ORF Insert Sequence:

ATGCGGCAGT CCCAGCAGGA GCGCAGGATT GCCGTGCAGC TCATGCATGT TCGGCATGAA 
AAGGAAGTTT TATGGCAAAA CAGAATTTTC AGAGAAAAAC AACATGAGGA AAGACGACTT 
AAAGATTTCC AGGATGCTCT TGATCGAGAA GCGGCTTTGG CAAAACAAGC CAAGATTGAC 
TTTGAAGAAC AATTCCTTAA AGAAAAGAGA TTTCATGATC AGATTGCTGT GGAAAGAGCT 
CAAGCTCGTT ATGAAAAGCA TTATTCAGTA TGTGCAGAAA TTTTGGATCA AATAGTTGAT 
TTGTCCACTA AAGTGGCAGA CTATCGAATG TTGACAAATA ATCTGATTCC GTATAAGTTG 
ATGCATGATT GGAAGGAACT ATTTTTTAAT GCAAAACCCA TATATGAACA AGCCTCTGTT 
AAGACACTAC CTGCTGACCC CTCAAGAGAA CAACTTACAG AACTGGAGAA AAGGGACTTG 
CTAGATACCA ATGATTATGA AGAATATAAG AACATGGTTG GAGAGTGGGC CTTACCAGAA 
GAAATGGTTG ACAATTTACC ACCCTCCAAC AATTGCATAC TGGGCCATAT TCTTCACAGG 
CTAGTTGAAA AATCTCTTCC TCCTCGAGCG GAATCAACAA CACCTGAATT ACCTTCATTT 
GCTGTTAAAG GATGCTTATT GGGGAAAACA TTAAGTGGAA AAACTACCAT TTTAAGGTCT 
CTACAAAAAG ACTTTCCTAT ACAGATACTT TCTATTGACA CTCTTGTCCA AGAAGCTATC 
CAAGCATTTC ATGACAATGA AAAAGTCAGT GAGGTTCTAC CAATTCAGAA AAAAGATGAA 
GAAGATGCTC TACCAGTTCT GCAAGAGGAG ATTAAAGAAA GCCAGGATCC ACAACATGTA 
TTTTCAGCTG GTCCAGTTTC AGATGAAGTA TTACCAGAAA CAGAAGGTGA AACAATACTT 
AGTGGTAATG CTGATAAAAC ACCAAAAGCT GAAGAAGTCA AATCAAGTGA TAGTTTCTTA 
AAACTCACTA CACGTGCTCA GCTTGGTGCA AAATCAGAAC AGTTGCTGAA GAAAGGAAAG 
AGCATTCCTG ATGTGCTGCT TGTTGACATC ATGGTAAATG CTATTAACAA GATACCTGTG 
AATCAAGACT GTATCCTAGA TGGTTTTCCA ATGACTTTAA ACCAAGCACA GCTTCTGGAA 
GAAGCTCTTA CAGGCTGCAA TAGAAACCTC ACAGAAGTGG AAAGAAAAAA AGCACAAAAA 
TCCACATTGG CTATTGATCC TGCGACTTCC AAAGAAATAC CTCTTCCCTC TCCTGCATTT 
GATTTTGTCA TATTATTAGA TGTTTCAGAT ACTTCCTCAA TGAGTCGCAT GAATGATATT 
ATAGCTGAAG AATTGTCCTA TAAAACTGCT CACGAAGATA TCAGTCAACG TGTAGCTGCT 
GAAAACCAAG ATAAGGATGG AGACCAAAAT TTAAGAGACC AGATACAACA TAGAATTATA 
GGCTTCTTGG ACAACTGGCC TTTATTGGAG CAATGGTTTT CAGAGCCAGA AAATATTTTG 
ATAAAAATCA ATGCTGAAAT AGATAAGGAA TCTTTATGTG AAAAAGTAAA AGAAATTCTT 
ACGACTGAAA TAGCAAAAAA AAAGAATAAA GTTGAGAAGA AATTAGAAGA AAAGGAAGCT 
GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG CTTCCACTTC CTACACCTCC TCCTGCTCCT 
CCTCCTGAAC CAGAAAAAGA GAAGGAAATT CATCAAAGCC ATGTGCCTTC AAAAACTCCT 
ACTGCAAAAG GAAAACCTCA ATCAGAAGCC CCGCATGGTA AGCAAGAATC TCTTCAGGAA 
GGAAAAGGGA AGAAAGGTGA GACCGCACTC AAAAGAAAAG GTTCTCCTAA AGGAAAATCA 
TCAGGAGGAA AAGTACCAGT AAAGAAATCA CCTGCTGACT CTACAGATAC ATCACCTGTT 
GCAATAGTGC CACAGCCACC TAAGCCAGGA TCAGAAGAAT GGGTCTATGT GAATGAACCA 
GTTCCTGAGG AAATGCCTTC GTTTTTAGTA CCTTACTGGG AACTAATAGA AAATTCCTAT 
ATAAACACCA TCAAAACAGT ACTCAGGCAT CTGAGGGAAG ATCAGCATAC TGTGCTTGCT 
TACTTATATG AGATTAGAAC AAGTTTCCAG GAGTTTCTAA AGCGTCCAGA TCACAAGCAA 
GATTTTGTAG CTCAATGGCA GGCTGATTTC AACTCCCTTC CTGATGACCT GTGGGATGAT 
GAGGAAACAA AGGCTGAACT ACATCAACGA GTGAATGATC TGCGAGACCG CCTGTGGGAC 
ATTTGTGATG TCCGGAAGGA AGAGGCAGAG CAGGAGCGGC TTGACATCAT TAATAAGAGC 
TGGTTACAGG ACACTCTTGG AATGACAATG AACCATTTCT TTTCCCTGAT GCAGGCAGAA 
CTGAACCGTT TCCAAGATAC AAAGAGACTC CTTCAAGATT ATTACTGGGG AATGGAAAGT 
AAAATCCCAG TAGAGGACAA CAAGAGATTT ACTCAAATCC CTTTGGTCCA ACTGGATAGT 
AAAGACAATT CCGAAAGCCA GCTTAGAATT CCTCTAGTTC CTCGAATATC CATTTCTCTG 
GAAACAGTTA CACCCAAACC AAAAACAAAA TCAGTACTGA AGAGCAAGAC GGATAACTCC 
CTAGAAAACG TTGAGTCCAA CTTTGAGGCC GATGAAAAGT TGGTCATGGA CACCTGGCAG 
CAGGCTTCTT TAGCAGTATC TCACATGGTG GCTGCTGAAA TTCATCAGAG GCTTATGGAA 
GAAGAAAAAG AAAACCAGCC AGCAGACCCC AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT 
AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA AAACAGGAAG ACAAAAAACC CAAAGGTAAA 
TCATCACCTA TGGCAGAAGC AACTCCTGTC ATAGTAACAA CAGAGGAAAT TGCTGAAATC 
AAAAGGAAAA ATGAACTGAG GGTCAAAATA AAAGAAGAAC ACCTTGCTGC CTTGCAATTT 
GAAGAAATAG CCACGCAATT TCGACTTGAA CTGATAAAGA CAAAAGCATT GGCTCTTCTT 
GAAGATTTAG TAACAAAGGT GGTTGATGTA TATAAACTCA TGGAAAAATG GCTTGGTGAG 
AGGTATTTGA ATGAAATGGC CAGTATAGAA AAATTAACTG ACGTAGCTCG CTATCACATT 
GAAACATCTA CAAAAATTCA GAATGAACTT TATTTAAGCC AAGAAGACTT CTTCATTAAT 
GGCAATATAA AAGTCTTCCC AGATCCTCCC CCACCAATAC GTCCTCCACC TGTAGAAAAG 
GAAGAAGATG GCACCCTGAC CATTGAACAG CTTGACAGTC TTCGAGATCA GTTCTTAGAT 
ATGGCACCTA ACGGCATAAT AGGAAATAAA GCATTTACTG ACATTCTGAT CGATTTGGTG 
ACCCTGAACC TTGGCACAAA CAACTTTCCT AGTAGTTGGA TGCACCTTAC CCAACCTGAA 
TTACAGGAAT TAACATCTTT ATTAACAGTC AACTCCGAGT TCGTGGACTG GCGGAAGTTC 
CTGTTAGTAA CCTCAATGCC TTGGCCCGTT CCCTCGGAGG AGGAGCTCCT TGAGACCCTT 
CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG TTGGGCACCA TCACTTTCGA GCAGTATATG 
CAGGCTGGTC TGTGGTTTAC AGGAGATGAA GATATAAAAA TTCCAGAAAA TCCTCTTGAA 
CCCCTTCCAT TTAATAGGCA GGAGCATCTT ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC 
TATAAGAAGG ATCCACCCCA GCTTGACTAC ACACAGATGC TGCTTTACTT TGCTTGCCAC 
CCAGACACCG TGGAAGGAGC CTACAGGGCC CTCAGTGTGG CTGTTGGAAC TCATGTCTTC 
CAACAAGTCA AAGCTTCCAT TCCAAGTGCA GAAAAGACCT CCTCAACTGA TGCAGGTCCA 
GCTGAGGAAT TTCCTGAACC TGAGGGAAAT GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC 
GACAGGGAGA AAAGGGAACA GAAGGATGAA GAAATCCCTG AAAATGCAAA CAATGAAAAG 
ATGTCCATGG AAACACTACT CAAAGTGTTC AAAGGGGGAA GTGAAGCACA GGACTCCAAC 
AGATTTGCCA GCCACCTAAA GATAGAGAAC ATTTATGCAG AGGGCTTCAT AAAAACATTT 
CAAGACCTAG GTGCCAAGAA CCTGGAGCCA ATTGAAGTCA CTGTTCTCTT GAAGCATCCT 
TTTATTCAAG ACCTGATTTC AAATTATTCA GACTATAAGT TTCCTGTACA CATCATCTAT 
GTAGTGGAAA TGATAGTGTG TGCAAAAGGT TGGGCACCAC TTCCATCAGG AAAACAGTGT 
GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_024356633.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024212401.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc106442
Clone ID Related Accession (Same CDS sequence)	XM_024356633.1
Accession Version	XM_024356633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAGTTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCGTT 
CCCTCGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTCGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATAAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGC CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGGAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACAGGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAC AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024212401.1
CDS	150..5657
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_024356633.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356633.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAGTTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCGTT 
CCCTCGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTCGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATAAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGC CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGGAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACAGGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAC AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_024356634.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024212402.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OPc106443
Clone ID Related Accession (Same CDS sequence)	XM_024356634.1
Accession Version	XM_024356634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAGTTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCGTT 
CCCTCGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTCGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATAAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGC CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGGAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACAGGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAC AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGATAT TAAAATAATT CTCCAAAGGA GTGAACATGT ACAAGGAAGT 
GATGGAGAGA GATCACCTTC AAGACATACA GAGGAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024212402.1
CDS	150..5654
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_024356634.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356634.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAGTTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCGTT 
CCCTCGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTCGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATAAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGC CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGGAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACAGGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAC AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGATAT TAAAATAATT CTCCAAAGGA GTGAACATGT ACAAGGAAGT 
GATGGAGAGA GATCACCTTC AAGACATACA GAGGAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_024356635.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024212403.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OPc106444
Clone ID Related Accession (Same CDS sequence)	XM_024356635.1
Accession Version	XM_024356635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGG TAATGCTGAT AAAACACCAA AAGCTGAAGA AGTCAAATCA 
AGTGATAGTT TCTTAAAACT CACTACACGT GCTCAGCTTG GTGCAAAATC AGAACAGTTG 
CTGAAGAAAG GAAAGAGCAT TCCTGATGTG CTGCTTGTTG ACATCATGGT AAATGCTATT 
AACAAGATAC CTGTGAATCA AGACTGTATC CTAGATGGTT TTCCAATGAC TTTAAACCAA 
GCACAGCTTC TGGAAGAAGC TCTTACAGGC TGCAATAGAA ACCTCACAGA AGTGGAAAGA 
AAAAAAGCAC AAAAATCCAC ATTGGCTATT GATCCTGCGA CTTCCAAAGA AATACCTCTT 
CCCTCTCCTG CATTTGATTT TGTCATATTA TTAGATGTTT CAGATACTTC CTCAATGAGT 
CGCATGAATG ATATTATAGC TGAAGAATTG TCCTATAAAA CTGCTCACGA AGATATCAGT 
CAACGTGTAG CTGCTGAAAA CCAAGATAAG GATGGAGACC AAAATTTAAG AGACCAGATA 
CAACATAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTAT TGGAGCAATG GTTTTCAGAG 
CCAGAAAATA TTTTGATAAA AATCAATGCT GAAATAGATA AGGAATCTTT ATGTGAAAAA 
GTAAAAGAAA TTCTTACGAC TGAAATAGCA AAAAAAAAGA ATAAAGTTGA GAAGAAATTA 
GAAGAAAAGG AAGCTGAGAA AAAAGCAGCA GCTTCCCTGG CTGAGCTTCC ACTTCCTACA 
CCTCCTCCTG CTCCTCCTCC TGAACCAGAA AAAGAGAAGG AAATTCATCA AAGCCATGTG 
CCTTCAAAAA CTCCTACTGC AAAAGGAAAA CCTCAATCAG AAGCCCCGCA TGGTAAGCAA 
GAATCTCTTC AGGAAGGAAA AGGGAAGAAA GGTGAGACCG CACTCAAAAG AAAAGGTTCT 
CCTAAAGGAA AATCATCAGG AGGAAAAGTA CCAGTAAAGA AATCACCTGC TGACTCTACA 
GATACATCAC CTGTTGCAAT AGTGCCACAG CCACCTAAGC CAGGATCAGA AGAATGGGTC 
TATGTGAATG AACCAGTTCC TGAGGAAATG CCTTCGTTTT TAGTACCTTA CTGGGAACTA 
ATAGAAAATT CCTATATAAA CACCATCAAA ACAGTACTCA GGCATCTGAG GGAAGATCAG 
CATACTGTGC TTGCTTACTT ATATGAGATT AGAACAAGTT TCCAGGAGTT TCTAAAGCGT 
CCAGATCACA AGCAAGATTT TGTAGCTCAA TGGCAGGCTG ATTTCAACTC CCTTCCTGAT 
GACCTGTGGG ATGATGAGGA AACAAAGGCT GAACTACATC AACGAGTGAA TGATCTGCGA 
GACCGCCTGT GGGACATTTG TGATGTCCGG AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC 
ATCATTAATG AGAGCTGGTT ACAGGACACT CTTGGAATGA CAATGAACCA TTTCTTTTCC 
CTGATGCAGG CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC 
TGGGGAATGG AAAGTAAAAT CCCAGTAGAG GACAACAAGA GATTTACTCA AATCCCTTTG 
GTCCAACTGG ATAGTAAAGA CAATTCCGAA AGCCAGCTTA GAATTCCTCT AGTTCCTCGA 
ATATCCATTT CTCTGGAAAC AGTTACACCC AAACCAAAAA CAAAATCAGT ACTGAAGAGC 
AAGACGGATA ACTCCCTAGA AAACGTTGAG TCCAACTTTG AGGCCGATGA AAAGTTGGTC 
ATGGACACCT GGCAGCAGGC TTCTTTAGCA GTATCTCACA TGGTGGCTGC TGAAATTCAT 
CAGAGGCTTA TGGAAGAAGA AAAAGAAAAC CAGCCAGCAG ACCCCAAAGA AAAATCTCCT 
CAGATGGGTG CAAATAAAAA AGTCAAAAAG GAGCCACCCA AGAAAAAACA GGAAGACAAA 
AAACCCAAAG GTAAATCATC ACCTATGGCA GAAGCAACTC CTGTCATAGT AACAACAGAG 
GAAATTGCTG AAATCAAAAG GAAAAATGAA CTGAGGGTCA AAATAAAAGA AGAACACCTT 
GCTGCCTTGC AATTTGAAGA AATAGCCACG CAATTTCGAC TTGAACTGAT AAAGACAAAA 
GCATTGGCTC TTCTTGAAGA TTTAGTAACA AAGGTGGTTG ATGTATATAA ACTCATGGAA 
AAATGGCTTG GTGAGAGGTA TTTGAATGAA ATGGCCAGTA TAGAAAAATT AACTGACGTA 
GCTCGCTATC ACATTGAAAC ATCTACAAAA ATTCAGAATG AACTTTATTT AAGCCAAGAA 
GACTTCTTCA TTAATGGCAA TATAAAAGTC TTCCCAGATC CTCCCCCACC AATACGTCCT 
CCACCTGTAG AAAAGGAAGA AGATGGCACC CTGACCATTG AACAGCTTGA CAGTCTTCGA 
GATCAGTTCT TAGATATGGC ACCTAACGGC ATAATAGGAA ATAAAGCATT TACTGACATT 
CTGATCGATT TGGTGACCCT GAACCTTGGC ACAAACAACT TTCCTAGTAG TTGGATGCAC 
CTTACCCAAC CTGAATTACA GGAATTAACA TCTTTATTAA CAGTCAACTC CGAGTTCGTG 
GACTGGCGGA AGTTCCTGTT AGTAACCTCA ATGCCTTGGC CCGTTCCCTC GGAGGAGGAG 
CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT GTGGATAAGG AGCAGTTGGG CACCATCACT 
TTCGAGCAGT ATATGCAGGC TGGTCTGTGG TTTACAGGAG ATGAAGATAT AAAAATTCCA 
GAAAATCCTC TTGAACCCCT TCCATTTAAT AGGCAGGAGC ATCTTATAGA GTTTTTCTTT 
AGGCTATTTG CTGACTATAA GAAGGATCCA CCCCAGCTTG ACTACACACA GATGCTGCTT 
TACTTTGCTT GCCACCCAGA CACCGTGGAA GGAGCCTACA GGGCCCTCAG TGTGGCTGTT 
GGAACTCATG TCTTCCAACA AGTCAAAGCT TCCATTCCAA GTGCAGAAAA GACCTCCTCA 
ACTGATGCAG GTCCAGCTGA GGAATTTCCT GAACCTGAGG GAAATGCTGC AAGAGAAGAA 
AGGAAATTAA AAGACGACAG GGAGAAAAGG GAACAGAAGG ATGAAGAAAT CCCTGAAAAT 
GCAAACAATG AAAAGATGTC CATGGAAACA CTACTCAAAG TGTTCAAAGG GGGAAGTGAA 
GCACAGGACT CCAACAGATT TGCCAGCCAC CTAAAGATAG AGAACATTTA TGCAGAGGGC 
TTCATAAAAA CATTTCAAGA CCTAGGTGCC AAGAACCTGG AGCCAATTGA AGTCGCTGTT 
CTCTTGAAGC ATCCTTTTAT TCAAGACCTG ATTTCAAATT ATTCAGACTA TAAGTTTCCT 
GTACACATCA TCTATGTAGT GGAAATGATA GTGTGTGCAA AAGGTTGGGC ACCACTTCCA 
TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024212403.1
CDS	150..5642
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_024356635.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356635.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGG TAATGCTGAT AAAACACCAA AAGCTGAAGA AGTCAAATCA 
AGTGATAGTT TCTTAAAACT CACTACACGT GCTCAGCTTG GTGCAAAATC AGAACAGTTG 
CTGAAGAAAG GAAAGAGCAT TCCTGATGTG CTGCTTGTTG ACATCATGGT AAATGCTATT 
AACAAGATAC CTGTGAATCA AGACTGTATC CTAGATGGTT TTCCAATGAC TTTAAACCAA 
GCACAGCTTC TGGAAGAAGC TCTTACAGGC TGCAATAGAA ACCTCACAGA AGTGGAAAGA 
AAAAAAGCAC AAAAATCCAC ATTGGCTATT GATCCTGCGA CTTCCAAAGA AATACCTCTT 
CCCTCTCCTG CATTTGATTT TGTCATATTA TTAGATGTTT CAGATACTTC CTCAATGAGT 
CGCATGAATG ATATTATAGC TGAAGAATTG TCCTATAAAA CTGCTCACGA AGATATCAGT 
CAACGTGTAG CTGCTGAAAA CCAAGATAAG GATGGAGACC AAAATTTAAG AGACCAGATA 
CAACATAGAA TTATAGGCTT CTTGGACAAC TGGCCTTTAT TGGAGCAATG GTTTTCAGAG 
CCAGAAAATA TTTTGATAAA AATCAATGCT GAAATAGATA AGGAATCTTT ATGTGAAAAA 
GTAAAAGAAA TTCTTACGAC TGAAATAGCA AAAAAAAAGA ATAAAGTTGA GAAGAAATTA 
GAAGAAAAGG AAGCTGAGAA AAAAGCAGCA GCTTCCCTGG CTGAGCTTCC ACTTCCTACA 
CCTCCTCCTG CTCCTCCTCC TGAACCAGAA AAAGAGAAGG AAATTCATCA AAGCCATGTG 
CCTTCAAAAA CTCCTACTGC AAAAGGAAAA CCTCAATCAG AAGCCCCGCA TGGTAAGCAA 
GAATCTCTTC AGGAAGGAAA AGGGAAGAAA GGTGAGACCG CACTCAAAAG AAAAGGTTCT 
CCTAAAGGAA AATCATCAGG AGGAAAAGTA CCAGTAAAGA AATCACCTGC TGACTCTACA 
GATACATCAC CTGTTGCAAT AGTGCCACAG CCACCTAAGC CAGGATCAGA AGAATGGGTC 
TATGTGAATG AACCAGTTCC TGAGGAAATG CCTTCGTTTT TAGTACCTTA CTGGGAACTA 
ATAGAAAATT CCTATATAAA CACCATCAAA ACAGTACTCA GGCATCTGAG GGAAGATCAG 
CATACTGTGC TTGCTTACTT ATATGAGATT AGAACAAGTT TCCAGGAGTT TCTAAAGCGT 
CCAGATCACA AGCAAGATTT TGTAGCTCAA TGGCAGGCTG ATTTCAACTC CCTTCCTGAT 
GACCTGTGGG ATGATGAGGA AACAAAGGCT GAACTACATC AACGAGTGAA TGATCTGCGA 
GACCGCCTGT GGGACATTTG TGATGTCCGG AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC 
ATCATTAATG AGAGCTGGTT ACAGGACACT CTTGGAATGA CAATGAACCA TTTCTTTTCC 
CTGATGCAGG CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC 
TGGGGAATGG AAAGTAAAAT CCCAGTAGAG GACAACAAGA GATTTACTCA AATCCCTTTG 
GTCCAACTGG ATAGTAAAGA CAATTCCGAA AGCCAGCTTA GAATTCCTCT AGTTCCTCGA 
ATATCCATTT CTCTGGAAAC AGTTACACCC AAACCAAAAA CAAAATCAGT ACTGAAGAGC 
AAGACGGATA ACTCCCTAGA AAACGTTGAG TCCAACTTTG AGGCCGATGA AAAGTTGGTC 
ATGGACACCT GGCAGCAGGC TTCTTTAGCA GTATCTCACA TGGTGGCTGC TGAAATTCAT 
CAGAGGCTTA TGGAAGAAGA AAAAGAAAAC CAGCCAGCAG ACCCCAAAGA AAAATCTCCT 
CAGATGGGTG CAAATAAAAA AGTCAAAAAG GAGCCACCCA AGAAAAAACA GGAAGACAAA 
AAACCCAAAG GTAAATCATC ACCTATGGCA GAAGCAACTC CTGTCATAGT AACAACAGAG 
GAAATTGCTG AAATCAAAAG GAAAAATGAA CTGAGGGTCA AAATAAAAGA AGAACACCTT 
GCTGCCTTGC AATTTGAAGA AATAGCCACG CAATTTCGAC TTGAACTGAT AAAGACAAAA 
GCATTGGCTC TTCTTGAAGA TTTAGTAACA AAGGTGGTTG ATGTATATAA ACTCATGGAA 
AAATGGCTTG GTGAGAGGTA TTTGAATGAA ATGGCCAGTA TAGAAAAATT AACTGACGTA 
GCTCGCTATC ACATTGAAAC ATCTACAAAA ATTCAGAATG AACTTTATTT AAGCCAAGAA 
GACTTCTTCA TTAATGGCAA TATAAAAGTC TTCCCAGATC CTCCCCCACC AATACGTCCT 
CCACCTGTAG AAAAGGAAGA AGATGGCACC CTGACCATTG AACAGCTTGA CAGTCTTCGA 
GATCAGTTCT TAGATATGGC ACCTAACGGC ATAATAGGAA ATAAAGCATT TACTGACATT 
CTGATCGATT TGGTGACCCT GAACCTTGGC ACAAACAACT TTCCTAGTAG TTGGATGCAC 
CTTACCCAAC CTGAATTACA GGAATTAACA TCTTTATTAA CAGTCAACTC CGAGTTCGTG 
GACTGGCGGA AGTTCCTGTT AGTAACCTCA ATGCCTTGGC CCGTTCCCTC GGAGGAGGAG 
CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT GTGGATAAGG AGCAGTTGGG CACCATCACT 
TTCGAGCAGT ATATGCAGGC TGGTCTGTGG TTTACAGGAG ATGAAGATAT AAAAATTCCA 
GAAAATCCTC TTGAACCCCT TCCATTTAAT AGGCAGGAGC ATCTTATAGA GTTTTTCTTT 
AGGCTATTTG CTGACTATAA GAAGGATCCA CCCCAGCTTG ACTACACACA GATGCTGCTT 
TACTTTGCTT GCCACCCAGA CACCGTGGAA GGAGCCTACA GGGCCCTCAG TGTGGCTGTT 
GGAACTCATG TCTTCCAACA AGTCAAAGCT TCCATTCCAA GTGCAGAAAA GACCTCCTCA 
ACTGATGCAG GTCCAGCTGA GGAATTTCCT GAACCTGAGG GAAATGCTGC AAGAGAAGAA 
AGGAAATTAA AAGACGACAG GGAGAAAAGG GAACAGAAGG ATGAAGAAAT CCCTGAAAAT 
GCAAACAATG AAAAGATGTC CATGGAAACA CTACTCAAAG TGTTCAAAGG GGGAAGTGAA 
GCACAGGACT CCAACAGATT TGCCAGCCAC CTAAAGATAG AGAACATTTA TGCAGAGGGC 
TTCATAAAAA CATTTCAAGA CCTAGGTGCC AAGAACCTGG AGCCAATTGA AGTCGCTGTT 
CTCTTGAAGC ATCCTTTTAT TCAAGACCTG ATTTCAAATT ATTCAGACTA TAAGTTTCCT 
GTACACATCA TCTATGTAGT GGAAATGATA GTGTGTGCAA AAGGTTGGGC ACCACTTCCA 
TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953432.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808921.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

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CloneID	OPc106445
Clone ID Related Accession (Same CDS sequence)	XM_016953432.2
Accession Version	XM_016953432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT GGAGAATGCC AAGGCGTAGC TGTCCCAAGT CATTTGCAAA GGCATTTTCC 
AGTGGCTATC TACTTGGAGA AGTTCTACAC AAGTTTGAAC TTCAGGATGA TTTTTCAGAA 
TTTTTGGACA GCAGGGTTTC AAGTGCCAAA CTTAATAATT TTTCTCGCTT GGAGCCAACA 
CTTCACCTTC TGGGTGTGCA GTTTGATCAG AATGTGGCCC ATGGCATCAT CACAGAAAAG 
CCTGGGGTGG CAACAAAGCT GTTATATCAA TTGTACATTG CTCTTCAGAA AAAGAAGAAA 
AGTGGACTGA CTGGAGTGGA GATGCAAACC ATGCAACGTC TGACAAATTT AAGACTTCAA 
AACTTGAAAA GTGATACTTT TCAAGAGAGA CTTAGACACA TGATACCACG TCAAACTGAT 
TTCAATCTGA TGCGGATTAC ATACAGGTTT CAAGAAAAAT ATAAACACGT GAAAGAAGAT 
CTTGCCCATT TGCATTTTGA GAAACTTGAA AGATTTCAAA AACTCAAGGA AGAGCAAAGA 
TGTTTTGATA TCGAAAAGCA ATACTTAAAC AGAAGACGAC AAAATGAAAT AATGGCCAAA 
ATCCAAGCAG CTATTATACA GATTCCTAAA CCTGCATCAA ATCGTACTTT GAAAGCACTC 
GAGGCCCGAA AAATGATGAA AAAGAAAAAA GAGGCAGAAG ATGTGGCTGA TGAAATTAAG 
AAGTTCGAAG CATTAATAAA AAAGGATCTC CAAGCAAAAG AAAGTGCATC CAAGACTTCT 
TTAGATACAG CAGGCCAGAC AACCACCGAT TTGTTAAATA CTTACTCAGA TGACGAGTAC 
ATTAAAAAAA TCCAGAAGCG CCTTGAAGAA GATGCTTTTG CACGAGAGCA AAGGGAGAAA 
AGACGGCGGA AATTGTTAAT GGACCAGTTA ATAGCCCACG AAGCACAAGA GGAGGCTTAT 
CGGGAGGAAC AGCTGATTAA CCGGCTGATG CGGCAGTCCC AGCAGGAGCG CAGGATTGCC 
GTGCAGCTCA TGCATGTTCG GCATGAAAAG GAAGTTTTAT GGCAAAACAG AATTTTCAGA 
GAAAAACAAC ATGAGGAAAG ACGACTTAAA GATTTCCAGG ATGCTCTTGA TCGAGAAGCG 
GCTTTGGCAA AACAAGCCAA GATTGACTTT GAAGAACAAT TCCTTAAAGA AAAGAGATTT 
CATGATCAGA TTGCTGTGGA AAGAGCTCAA GCTCGTTATG AAAAGCATTA TTCAGTATGT 
GCAGAAATTT TGGATCAAAT AGTTGATTTG TCCACTAAAG TGGCAGACTA TCGAATGTTG 
ACAAATAATC TGATTCCGTA TAAGTTGATG CATGATTGGA AGGAACTATT TTTTAATGCA 
AAACCCATAT ATGAACAAGC CTCTGTTAAG ACACTACCTG CTGACCCCTC AAGAGAACAA 
CTTACAGAAC TGGAGAAAAG GGACTTGCTA GATACCAATG ATTATGAAGA ATATAAGATA 
TGGAGGAATA CAGCTGACCT CTCAGTGGAC AGAAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGTTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAAAGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATA 
CTTAGTGGTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATGGTAA ATGCTATTAA CAAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGCC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG 
GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA 
TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT 
GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA 
CCAGTTCCTG AGGAAATGCC TTCGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC 
TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGATCAGCA TACTGTGCTT 
GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC AGATCACAAG 
CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT 
GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG 
GACATTTGTG ATGTCCGGAA GGAAGAGGCA GAGCAGGAGC GGCTTGACAT CATTAATGAG 
AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA 
GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA 
AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCAAA TCCCTTTGGT CCAACTGGAT 
AGTAAAGACA ATTCCGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT 
CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGAGCAA GACGGATAAC 
TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG 
CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG 
GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA 
AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT 
AAATCATCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA 
ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA 
TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT 
CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT 
GAGAGGTATT TGAATGAAAT GGCCAGTATA GAAAAATTAA CTGACGTAGC TCGCTATCAC 
ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT 
AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCACCAA TACGTCCTCC ACCTGTAGAA 
AAGGAAGAAG ATGGCACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA 
GATATGGCAC CTAACGGCAT AATAGGAAAT AAAGCATTTA CTGACATTCT GATCGATTTG 
GTGACCCTGA ACCTTGGCAC AAACAACTTT CCTAGTAGTT GGATGCACCT TACCCAACCT 
GAATTACAGG AATTAACATC TTTATTAACA GTCAACTCCG AGTTCGTGGA CTGGCGGAAG 
TTCCTGTTAG TAACCTCAAT GCCTTGGCCC GTTCCCTCGG AGGAGGAGCT CCTTGAGACC 
CTTCAGAAGT TCAAGGCTGT GGATAAGGAG CAGTTGGGCA CCATCACTTT CGAGCAGTAT 
ATGCAGGCTG GTCTGTGGTT TACAGGAGAT GAAGATATAA AAATTCCAGA AAATCCTCTT 
GAACCCCTTC CATTTAATAG GCAGGAGCAT CTTATAGAGT TTTTCTTTAG GCTATTTGCT 
GACTATAAGA AGGATCCACC CCAGCTTGAC TACACACAGA TGCTGCTTTA CTTTGCTTGC 
CACCCAGACA CCGTGGAAGG AGCCTACAGG GCCCTCAGTG TGGCTGTTGG AACTCATGTC 
TTCCAACAAG TCAAAGCTTC CATTCCAAGT GCAGAAAAGA CCTCCTCAAC TGATGCAGGT 
CCAGCTGAGG AATTTCCTGA ACCTGAGGGA AATGCTGCAA GAGAAGAAAG GAAATTAAAA 
GACGACAGGG AGAAAAGGGA ACAGAAGGAT GAAGAAATCC CTGAAAATGC AAACAATGAA 
AAGATGTCCA TGGAAACACT ACTCAAAGTG TTCAAAGGGG GAAGTGAAGC ACAGGACTCC 
AACAGATTTG CCAGCCACCT AAAGATAGAG AACATTTATG CAGAGGGCTT CATAAAAACA 
TTTCAAGACC TAGGTGCCAA GAACCTGGAG CCAATTGAAG TCGCTGTTCT CTTGAAGCAT 
CCTTTTATTC AAGACCTGAT TTCAAATTAT TCAGACTATA AGTTTCCTGT ACACATCATC 
TATGTAGTGG AAATGATAGT GTGTGCAAAA GGTTGGGCAC CACTTCCATC AGGAAAACAG 
TGTGCAAGAT GGAGAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808921.2
CDS	65..5545
Translation	MFSWRMPRRSCPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953432.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953432.2

ORF Insert Sequence:

ATGTTCAGCT GGAGAATGCC AAGGCGTAGC TGTCCCAAGT CATTTGCAAA GGCATTTTCC 
AGTGGCTATC TACTTGGAGA AGTTCTACAC AAGTTTGAAC TTCAGGATGA TTTTTCAGAA 
TTTTTGGACA GCAGGGTTTC AAGTGCCAAA CTTAATAATT TTTCTCGCTT GGAGCCAACA 
CTTCACCTTC TGGGTGTGCA GTTTGATCAG AATGTGGCCC ATGGCATCAT CACAGAAAAG 
CCTGGGGTGG CAACAAAGCT GTTATATCAA TTGTACATTG CTCTTCAGAA AAAGAAGAAA 
AGTGGACTGA CTGGAGTGGA GATGCAAACC ATGCAACGTC TGACAAATTT AAGACTTCAA 
AACTTGAAAA GTGATACTTT TCAAGAGAGA CTTAGACACA TGATACCACG TCAAACTGAT 
TTCAATCTGA TGCGGATTAC ATACAGGTTT CAAGAAAAAT ATAAACACGT GAAAGAAGAT 
CTTGCCCATT TGCATTTTGA GAAACTTGAA AGATTTCAAA AACTCAAGGA AGAGCAAAGA 
TGTTTTGATA TCGAAAAGCA ATACTTAAAC AGAAGACGAC AAAATGAAAT AATGGCCAAA 
ATCCAAGCAG CTATTATACA GATTCCTAAA CCTGCATCAA ATCGTACTTT GAAAGCACTC 
GAGGCCCGAA AAATGATGAA AAAGAAAAAA GAGGCAGAAG ATGTGGCTGA TGAAATTAAG 
AAGTTCGAAG CATTAATAAA AAAGGATCTC CAAGCAAAAG AAAGTGCATC CAAGACTTCT 
TTAGATACAG CAGGCCAGAC AACCACCGAT TTGTTAAATA CTTACTCAGA TGACGAGTAC 
ATTAAAAAAA TCCAGAAGCG CCTTGAAGAA GATGCTTTTG CACGAGAGCA AAGGGAGAAA 
AGACGGCGGA AATTGTTAAT GGACCAGTTA ATAGCCCACG AAGCACAAGA GGAGGCTTAT 
CGGGAGGAAC AGCTGATTAA CCGGCTGATG CGGCAGTCCC AGCAGGAGCG CAGGATTGCC 
GTGCAGCTCA TGCATGTTCG GCATGAAAAG GAAGTTTTAT GGCAAAACAG AATTTTCAGA 
GAAAAACAAC ATGAGGAAAG ACGACTTAAA GATTTCCAGG ATGCTCTTGA TCGAGAAGCG 
GCTTTGGCAA AACAAGCCAA GATTGACTTT GAAGAACAAT TCCTTAAAGA AAAGAGATTT 
CATGATCAGA TTGCTGTGGA AAGAGCTCAA GCTCGTTATG AAAAGCATTA TTCAGTATGT 
GCAGAAATTT TGGATCAAAT AGTTGATTTG TCCACTAAAG TGGCAGACTA TCGAATGTTG 
ACAAATAATC TGATTCCGTA TAAGTTGATG CATGATTGGA AGGAACTATT TTTTAATGCA 
AAACCCATAT ATGAACAAGC CTCTGTTAAG ACACTACCTG CTGACCCCTC AAGAGAACAA 
CTTACAGAAC TGGAGAAAAG GGACTTGCTA GATACCAATG ATTATGAAGA ATATAAGATA 
TGGAGGAATA CAGCTGACCT CTCAGTGGAC AGAAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGTTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAAAGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATA 
CTTAGTGGTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATGGTAA ATGCTATTAA CAAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGCC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG 
GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA 
TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT 
GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA 
CCAGTTCCTG AGGAAATGCC TTCGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC 
TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGATCAGCA TACTGTGCTT 
GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC AGATCACAAG 
CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT 
GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG 
GACATTTGTG ATGTCCGGAA GGAAGAGGCA GAGCAGGAGC GGCTTGACAT CATTAATGAG 
AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA 
GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA 
AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCAAA TCCCTTTGGT CCAACTGGAT 
AGTAAAGACA ATTCCGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT 
CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGAGCAA GACGGATAAC 
TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG 
CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG 
GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA 
AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT 
AAATCATCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA 
ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA 
TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT 
CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT 
GAGAGGTATT TGAATGAAAT GGCCAGTATA GAAAAATTAA CTGACGTAGC TCGCTATCAC 
ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT 
AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCACCAA TACGTCCTCC ACCTGTAGAA 
AAGGAAGAAG ATGGCACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA 
GATATGGCAC CTAACGGCAT AATAGGAAAT AAAGCATTTA CTGACATTCT GATCGATTTG 
GTGACCCTGA ACCTTGGCAC AAACAACTTT CCTAGTAGTT GGATGCACCT TACCCAACCT 
GAATTACAGG AATTAACATC TTTATTAACA GTCAACTCCG AGTTCGTGGA CTGGCGGAAG 
TTCCTGTTAG TAACCTCAAT GCCTTGGCCC GTTCCCTCGG AGGAGGAGCT CCTTGAGACC 
CTTCAGAAGT TCAAGGCTGT GGATAAGGAG CAGTTGGGCA CCATCACTTT CGAGCAGTAT 
ATGCAGGCTG GTCTGTGGTT TACAGGAGAT GAAGATATAA AAATTCCAGA AAATCCTCTT 
GAACCCCTTC CATTTAATAG GCAGGAGCAT CTTATAGAGT TTTTCTTTAG GCTATTTGCT 
GACTATAAGA AGGATCCACC CCAGCTTGAC TACACACAGA TGCTGCTTTA CTTTGCTTGC 
CACCCAGACA CCGTGGAAGG AGCCTACAGG GCCCTCAGTG TGGCTGTTGG AACTCATGTC 
TTCCAACAAG TCAAAGCTTC CATTCCAAGT GCAGAAAAGA CCTCCTCAAC TGATGCAGGT 
CCAGCTGAGG AATTTCCTGA ACCTGAGGGA AATGCTGCAA GAGAAGAAAG GAAATTAAAA 
GACGACAGGG AGAAAAGGGA ACAGAAGGAT GAAGAAATCC CTGAAAATGC AAACAATGAA 
AAGATGTCCA TGGAAACACT ACTCAAAGTG TTCAAAGGGG GAAGTGAAGC ACAGGACTCC 
AACAGATTTG CCAGCCACCT AAAGATAGAG AACATTTATG CAGAGGGCTT CATAAAAACA 
TTTCAAGACC TAGGTGCCAA GAACCTGGAG CCAATTGAAG TCGCTGTTCT CTTGAAGCAT 
CCTTTTATTC AAGACCTGAT TTCAAATTAT TCAGACTATA AGTTTCCTGT ACACATCATC 
TATGTAGTGG AAATGATAGT GTGTGCAAAA GGTTGGGCAC CACTTCCATC AGGAAAACAG 
TGTGCAAGAT GGAGAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953430.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808919.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

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CloneID	OPc106446
Clone ID Related Accession (Same CDS sequence)	XM_016953430.2
Accession Version	XM_016953430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808919.2
CDS	150..5621
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953430.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953430.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_009449294.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009447569.3 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

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CloneID	OPc106447
Clone ID Related Accession (Same CDS sequence)	XM_009449294.3
Accession Version	XM_009449294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009449294.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009447569.3
CDS	150..5618
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_009449294.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009449294.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953431.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808920.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

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CloneID	OPc106448
Clone ID Related Accession (Same CDS sequence)	XM_016953431.2
Accession Version	XM_016953431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGGTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACAAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808920.2
CDS	150..5606
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953431.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953431.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGGTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACAAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_024356636.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024212404.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

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CloneID	OPc106449
Clone ID Related Accession (Same CDS sequence)	XM_024356636.1
Accession Version	XM_024356636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGGTTCT 
CCTAAAGGAA AATCATCAGG AGGAAAAGTA CCAGTAAAGA AATCACCTGC TGACTCTACA 
GATACATCAC CTGTTGCAAT AGTGCCACAG CCACCTAAGC CAGGATCAGA AGAATGGGTC 
TATGTGAATG AACCAGTTCC TGAGGAAATG CCTTCGTTTT TAGTACCTTA CTGGGAACTA 
ATAGAAAATT CCTATATAAA CACCATCAAA ACAGTACTCA GGCATCTGAG GGAAGATCAG 
CATACTGTGC TTGCTTACTT ATATGAGATT AGAACAAGTT TCCAGGAGTT TCTAAAGCGT 
CCAGATCACA AGCAAGATTT TGTAGCTCAA TGGCAGGCTG ATTTCAACTC CCTTCCTGAT 
GACCTGTGGG ATGATGAGGA AACAAAGGCT GAACTACATC AACGAGTGAA TGATCTGCGA 
GACCGCCTGT GGGACATTTG TGATGTCCGG AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC 
ATCATTAATG AGAGCTGGTT ACAGGACACT CTTGGAATGA CAATGAACCA TTTCTTTTCC 
CTGATGCAGG CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC 
TGGGGAATGG AAAGTAAAAT CCCAGTAGAG GACAACAAGA GATTTACTCA AATCCCTTTG 
GTCCAACTGG ATAGTAAAGA CAATTCCGAA AGCCAGCTTA GAATTCCTCT AGTTCCTCGA 
ATATCCATTT CTCTGGAAAC AGTTACACCC AAACCAAAAA CAAAATCAGT ACTGAAGAGC 
AAGACGGATA ACTCCCTAGA AAACGTTGAG TCCAACTTTG AGGCCGATGA AAAGTTGGTC 
ATGGACACCT GGCAGCAGGC TTCTTTAGCA GTATCTCACA TGGTGGCTGC TGAAATTCAT 
CAGAGGCTTA TGGAAGAAGA AAAAGAAAAC CAGCCAGCAG ACCCCAAAGA AAAATCTCCT 
CAGATGGGTG CAAATAAAAA AGTCAAAAAG GAGCCACCCA AGAAAAAACA GGAAGACAAA 
AAACCCAAAG GTAAATCATC ACCTATGGCA GAAGCAACTC CTGTCATAGT AACAACAGAG 
GAAATTGCTG AAATCAAAAG GAAAAATGAA CTGAGGGTCA AAATAAAAGA AGAACACCTT 
GCTGCCTTGC AATTTGAAGA AATAGCCACG CAATTTCGAC TTGAACTGAT AAAGACAAAA 
GCATTGGCTC TTCTTGAAGA TTTAGTAACA AAGGTGGTTG ATGTATATAA ACTCATGGAA 
AAATGGCTTG GTGAGAGGTA TTTGAATGAA ATGGCCAGTA TAGAAAAATT AACTGACGTA 
GCTCGCTATC ACATTGAAAC ATCTACAAAA ATTCAGAATG AACTTTATTT AAGCCAAGAA 
GACTTCTTCA TTAATGGCAA TATAAAAGTC TTCCCAGATC CTCCCCCACC AATACGTCCT 
CCACCTGTAG AAAAGGAAGA AGATGGCACC CTGACCATTG AACAGCTTGA CAGTCTTCGA 
GATCAGTTCT TAGATATGGC ACCTAACGGC ATAATAGGAA ATAAAGCATT TACTGACATT 
CTGATCGATT TGGTGACCCT GAACCTTGGC ACAAACAACT TTCCTAGTAG TTGGATGCAC 
CTTACCCAAC CTGAATTACA GGAATTAACA TCTTTATTAA CAGTCAACTC CGAGTTCGTG 
GACTGGCGGA AGTTCCTGTT AGTAACCTCA ATGCCTTGGC CCGTTCCCTC GGAGGAGGAG 
CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT GTGGATAAGG AGCAGTTGGG CACCATCACT 
TTCGAGCAGT ATATGCAGGC TGGTCTGTGG TTTACAGGAG ATGAAGATAT AAAAATTCCA 
GAAAATCCTC TTGAACCCCT TCCATTTAAT AGGCAGGAGC ATCTTATAGA GTTTTTCTTT 
AGGCTATTTG CTGACTATAA GAAGGATCCA CCCCAGCTTG ACTACACACA GATGCTGCTT 
TACTTTGCTT GCCACCCAGA CACCGTGGAA GGAGCCTACA GGGCCCTCAG TGTGGCTGTT 
GGAACTCATG TCTTCCAACA AGTCAAAGCT TCCATTCCAA GTGCAGAAAA GACCTCCTCA 
ACTGATGCAG GTCCAGCTGA GGAATTTCCT GAACCTGAGG GAAATGCTGC AAGAGAAGAA 
AGGAAATTAA AAGACGACAG GGAGAAAAGG GAACAGAAGG ATGAAGAAAT CCCTGAAAAT 
GCAAACAATG AAAAGATGTC CATGGAAACA CTACTCAAAG TGTTCAAAGG GGGAAGTGAA 
GCACAGGACT CCAACAGATT TGCCAGCCAC CTAAAGATAG AGAACATTTA TGCAGAGGGC 
TTCATAAAAA CATTTCAAGA CCTAGGTGCC AAGAACCTGG AGCCAATTGA AGTCGCTGTT 
CTCTTGAAGC ATCCTTTTAT TCAAGACCTG ATTTCAAATT ATTCAGACTA TAAGTTTCCT 
GTACACATCA TCTATGTAGT GGAAATGATA GTGTGTGCAA AAGGTTGGGC ACCACTTCCA 
TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024212404.1
CDS	150..5582
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_024356636.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356636.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGGTTCT 
CCTAAAGGAA AATCATCAGG AGGAAAAGTA CCAGTAAAGA AATCACCTGC TGACTCTACA 
GATACATCAC CTGTTGCAAT AGTGCCACAG CCACCTAAGC CAGGATCAGA AGAATGGGTC 
TATGTGAATG AACCAGTTCC TGAGGAAATG CCTTCGTTTT TAGTACCTTA CTGGGAACTA 
ATAGAAAATT CCTATATAAA CACCATCAAA ACAGTACTCA GGCATCTGAG GGAAGATCAG 
CATACTGTGC TTGCTTACTT ATATGAGATT AGAACAAGTT TCCAGGAGTT TCTAAAGCGT 
CCAGATCACA AGCAAGATTT TGTAGCTCAA TGGCAGGCTG ATTTCAACTC CCTTCCTGAT 
GACCTGTGGG ATGATGAGGA AACAAAGGCT GAACTACATC AACGAGTGAA TGATCTGCGA 
GACCGCCTGT GGGACATTTG TGATGTCCGG AAGGAAGAGG CAGAGCAGGA GCGGCTTGAC 
ATCATTAATG AGAGCTGGTT ACAGGACACT CTTGGAATGA CAATGAACCA TTTCTTTTCC 
CTGATGCAGG CAGAACTGAA CCGTTTCCAA GATACAAAGA GACTCCTTCA AGATTATTAC 
TGGGGAATGG AAAGTAAAAT CCCAGTAGAG GACAACAAGA GATTTACTCA AATCCCTTTG 
GTCCAACTGG ATAGTAAAGA CAATTCCGAA AGCCAGCTTA GAATTCCTCT AGTTCCTCGA 
ATATCCATTT CTCTGGAAAC AGTTACACCC AAACCAAAAA CAAAATCAGT ACTGAAGAGC 
AAGACGGATA ACTCCCTAGA AAACGTTGAG TCCAACTTTG AGGCCGATGA AAAGTTGGTC 
ATGGACACCT GGCAGCAGGC TTCTTTAGCA GTATCTCACA TGGTGGCTGC TGAAATTCAT 
CAGAGGCTTA TGGAAGAAGA AAAAGAAAAC CAGCCAGCAG ACCCCAAAGA AAAATCTCCT 
CAGATGGGTG CAAATAAAAA AGTCAAAAAG GAGCCACCCA AGAAAAAACA GGAAGACAAA 
AAACCCAAAG GTAAATCATC ACCTATGGCA GAAGCAACTC CTGTCATAGT AACAACAGAG 
GAAATTGCTG AAATCAAAAG GAAAAATGAA CTGAGGGTCA AAATAAAAGA AGAACACCTT 
GCTGCCTTGC AATTTGAAGA AATAGCCACG CAATTTCGAC TTGAACTGAT AAAGACAAAA 
GCATTGGCTC TTCTTGAAGA TTTAGTAACA AAGGTGGTTG ATGTATATAA ACTCATGGAA 
AAATGGCTTG GTGAGAGGTA TTTGAATGAA ATGGCCAGTA TAGAAAAATT AACTGACGTA 
GCTCGCTATC ACATTGAAAC ATCTACAAAA ATTCAGAATG AACTTTATTT AAGCCAAGAA 
GACTTCTTCA TTAATGGCAA TATAAAAGTC TTCCCAGATC CTCCCCCACC AATACGTCCT 
CCACCTGTAG AAAAGGAAGA AGATGGCACC CTGACCATTG AACAGCTTGA CAGTCTTCGA 
GATCAGTTCT TAGATATGGC ACCTAACGGC ATAATAGGAA ATAAAGCATT TACTGACATT 
CTGATCGATT TGGTGACCCT GAACCTTGGC ACAAACAACT TTCCTAGTAG TTGGATGCAC 
CTTACCCAAC CTGAATTACA GGAATTAACA TCTTTATTAA CAGTCAACTC CGAGTTCGTG 
GACTGGCGGA AGTTCCTGTT AGTAACCTCA ATGCCTTGGC CCGTTCCCTC GGAGGAGGAG 
CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT GTGGATAAGG AGCAGTTGGG CACCATCACT 
TTCGAGCAGT ATATGCAGGC TGGTCTGTGG TTTACAGGAG ATGAAGATAT AAAAATTCCA 
GAAAATCCTC TTGAACCCCT TCCATTTAAT AGGCAGGAGC ATCTTATAGA GTTTTTCTTT 
AGGCTATTTG CTGACTATAA GAAGGATCCA CCCCAGCTTG ACTACACACA GATGCTGCTT 
TACTTTGCTT GCCACCCAGA CACCGTGGAA GGAGCCTACA GGGCCCTCAG TGTGGCTGTT 
GGAACTCATG TCTTCCAACA AGTCAAAGCT TCCATTCCAA GTGCAGAAAA GACCTCCTCA 
ACTGATGCAG GTCCAGCTGA GGAATTTCCT GAACCTGAGG GAAATGCTGC AAGAGAAGAA 
AGGAAATTAA AAGACGACAG GGAGAAAAGG GAACAGAAGG ATGAAGAAAT CCCTGAAAAT 
GCAAACAATG AAAAGATGTC CATGGAAACA CTACTCAAAG TGTTCAAAGG GGGAAGTGAA 
GCACAGGACT CCAACAGATT TGCCAGCCAC CTAAAGATAG AGAACATTTA TGCAGAGGGC 
TTCATAAAAA CATTTCAAGA CCTAGGTGCC AAGAACCTGG AGCCAATTGA AGTCGCTGTT 
CTCTTGAAGC ATCCTTTTAT TCAAGACCTG ATTTCAAATT ATTCAGACTA TAAGTTTCCT 
GTACACATCA TCTATGTAGT GGAAATGATA GTGTGTGCAA AAGGTTGGGC ACCACTTCCA 
TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_024356637.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024212405.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

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CloneID	OPc106450
Clone ID Related Accession (Same CDS sequence)	XM_024356637.1
Accession Version	XM_024356637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCGAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTGA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGATATGGA GGAATACAGC TGACCTCTCA 
GTGGACAGAA ACATGGTTGG AGAGTGGGCC TTACCAGAAG AAATGGTTGA CAATTTACCA 
CCCTCCAACA ATTGCATACT GGGCCATATT CTTCACAGGC TAGTTGAAAA ATCTCTTCCT 
CCTCGAGCGG AATCAACAAC ACCTGAATTA CCTTCATTTG CTGTTAAAGG ATGCTTATTG 
GGGAAAACAT TAAGTGGAAA AACTACCATT TTAAGGTCTC TACAAAAAGA CTTTCCTATA 
CAGATACTTT CTATTGACAC TCTTGTCCAA GAAGCTATCC AAGCATTTCA TGACAATGAA 
AAAGTCAGTG AGGTTCTACC AATTCAGAAA AAAGATGAAG AAGATGCTCT ACCAGTTCTG 
CAAGAGGAGA TTAAAGAAAG CCAGGATCCA CAACATGTAT TTTCAGCTGG TCCAGTTTCA 
GATGAAGTAT TACCAGAAAC AGAAGGTGAA ACAATACTTA GTGGTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACAAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024212405.1
CDS	126..5462
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQEQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_024356637.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024356637.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCGAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTGA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGATATGGA GGAATACAGC TGACCTCTCA 
GTGGACAGAA ACATGGTTGG AGAGTGGGCC TTACCAGAAG AAATGGTTGA CAATTTACCA 
CCCTCCAACA ATTGCATACT GGGCCATATT CTTCACAGGC TAGTTGAAAA ATCTCTTCCT 
CCTCGAGCGG AATCAACAAC ACCTGAATTA CCTTCATTTG CTGTTAAAGG ATGCTTATTG 
GGGAAAACAT TAAGTGGAAA AACTACCATT TTAAGGTCTC TACAAAAAGA CTTTCCTATA 
CAGATACTTT CTATTGACAC TCTTGTCCAA GAAGCTATCC AAGCATTTCA TGACAATGAA 
AAAGTCAGTG AGGTTCTACC AATTCAGAAA AAAGATGAAG AAGATGCTCT ACCAGTTCTG 
CAAGAGGAGA TTAAAGAAAG CCAGGATCCA CAACATGTAT TTTCAGCTGG TCCAGTTTCA 
GATGAAGTAT TACCAGAAAC AGAAGGTGAA ACAATACTTA GTGGTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACAAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953436.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808925.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

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CloneID	OPc106451
Clone ID Related Accession (Same CDS sequence)	XM_016953436.2
Accession Version	XM_016953436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TCCAAGCAGC TATTATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
AAAGCACTCG AGGCCCGAAA AATGATGAAA AAGAAAAAAG AGGCAGAAGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGATAT GGAGGAATAC AGCTGACCTC TCAGTGGACA GAAACATGGT TGGAGAGTGG 
GCCTTACCAG AAGAAATGGT TGACAATTTA CCACCCTCCA ACAATTGCAT ACTGGGCCAT 
ATTCTTCACA GGCTAGTTGA AAAATCTCTT CCTCCTCGAG CGGAATCAAC AACACCTGAA 
TTACCTTCAT TTGCTGTTAA AGGATGCTTA TTGGGGAAAA CATTAAGTGG AAAAACTACC 
ATTTTAAGGT CTCTACAAAA AGACTTTCCT ATACAGATAC TTTCTATTGA CACTCTTGTC 
CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAAAGTCA GTGAGGTTCT ACCAATTCAG 
AAAAAAGATG AAGAAGATGC TCTACCAGTT CTGCAAGAGG AGATTAAAGA AAGCCAGGAT 
CCACAACATG TATTTTCAGC TGGTCCAGTT TCAGATGAAG TATTACCAGA AACAGAAGGT 
GAAACAATAC TTAGTGGTAA TGCTGATAAA ACACCAAAAG CTGAAGAAGT CAAATCAAGT 
GATAGTTTCT TAAAACTCAC TACACGTGCT CAGCTTGGTG CAAAATCAGA ACAGTTGCTG 
AAGAAAGGAA AGAGCATTCC TGATGTGCTG CTTGTTGACA TCATGGTAAA TGCTATTAAC 
AAGATACCTG TGAATCAAGA CTGTATCCTA GATGGTTTTC CAATGACTTT AAACCAAGCA 
CAGCTTCTGG AAGAAGCTCT TACAGGCTGC AATAGAAACC TCACAGAAGT GGAAAGAAAA 
AAAGCACAAA AATCCACATT GGCTATTGAT CCTGCGACTT CCAAAGAAAT ACCTCTTCCC 
TCTCCTGCAT TTGATTTTGT CATATTATTA GATGTTTCAG ATACTTCCTC AATGAGTCGC 
ATGAATGATA TTATAGCTGA AGAATTGTCC TATAAAACTG CTCACGAAGA TATCAGTCAA 
CGTGTAGCTG CTGAAAACCA AGATAAGGAT GGAGACCAAA ATTTAAGAGA CCAGATACAA 
CATAGAATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAAAAT CAATGCTGAA ATAGATAAGG AATCTTTATG TGAAAAAGTA 
AAAGAAATTC TTACGACTGA AATAGCAAAA AAAAAGAATA AAGTTGAGAA GAAATTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCAGCT TCCCTGGCTG AGCTTCCACT TCCTACACCT 
CCTCCTGCTC CTCCTCCTGA ACCAGAAAAA GAGAAGGAAA TTCATCAAAG CCATGTGCCT 
TCAAAAACTC CTACTGCAAA AGGAAAACCT CAATCAGAAG CCCCGCATGG TAAGCAAGAA 
TCTCTTCAGG AAGGAAAAGG GAAGAAAGGT GAGACCGCAC TCAAAAGAAA AGGTTCTCCT 
AAAGGAAAAT CATCAGGAGG AAAAGTACCA GTAAAGAAAT CACCTGCTGA CTCTACAGAT 
ACATCACCTG TTGCAATAGT GCCACAGCCA CCTAAGCCAG GATCAGAAGA ATGGGTCTAT 
GTGAATGAAC CAGTTCCTGA GGAAATGCCT TCGTTTTTAG TACCTTACTG GGAACTAATA 
GAAAATTCCT ATATAAACAC CATCAAAACA GTACTCAGGC ATCTGAGGGA AGATCAGCAT 
ACTGTGCTTG CTTACTTATA TGAGATTAGA ACAAGTTTCC AGGAGTTTCT AAAGCGTCCA 
GATCACAAGC AAGATTTTGT AGCTCAATGG CAGGCTGATT TCAACTCCCT TCCTGATGAC 
CTGTGGGATG ATGAGGAAAC AAAGGCTGAA CTACATCAAC GAGTGAATGA TCTGCGAGAC 
CGCCTGTGGG ACATTTGTGA TGTCCGGAAG GAAGAGGCAG AGCAGGAGCG GCTTGACATC 
ATTAATGAGA GCTGGTTACA GGACACTCTT GGAATGACAA TGAACCATTT CTTTTCCCTG 
ATGCAGGCAG AACTGAACCG TTTCCAAGAT ACAAAGAGAC TCCTTCAAGA TTATTACTGG 
GGAATGGAAA GTAAAATCCC AGTAGAGGAC AACAAGAGAT TTACTCAAAT CCCTTTGGTC 
CAACTGGATA GTAAAGACAA TTCCGAAAGC CAGCTTAGAA TTCCTCTAGT TCCTCGAATA 
TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT GAAGAGCAAG 
ACGGATAACT CCCTAGAAAA CGTTGAGTCC AACTTTGAGG CCGATGAAAA GTTGGTCATG 
GACACCTGGC AGCAGGCTTC TTTAGCAGTA TCTCACATGG TGGCTGCTGA AATTCATCAG 
AGGCTTATGG AAGAAGAAAA AGAAAACCAG CCAGCAGACC CCAAAGAAAA ATCTCCTCAG 
ATGGGTGCAA ATAAAAAAGT CAAAAAGGAG CCACCCAAGA AAAAACAGGA AGACAAAAAA 
CCCAAAGGTA AATCATCACC TATGGCAGAA GCAACTCCTG TCATAGTAAC AACAGAGGAA 
ATTGCTGAAA TCAAAAGGAA AAATGAACTG AGGGTCAAAA TAAAAGAAGA ACACCTTGCT 
GCCTTGCAAT TTGAAGAAAT AGCCACGCAA TTTCGACTTG AACTGATAAA GACAAAAGCA 
TTGGCTCTTC TTGAAGATTT AGTAACAAAG GTGGTTGATG TATATAAACT CATGGAAAAA 
TGGCTTGGTG AGAGGTATTT GAATGAAATG GCCAGTATAG AAAAATTAAC TGACGTAGCT 
CGCTATCACA TTGAAACATC TACAAAAATT CAGAATGAAC TTTATTTAAG CCAAGAAGAC 
TTCTTCATTA ATGGCAATAT AAAAGTCTTC CCAGATCCTC CCCCACCAAT ACGTCCTCCA 
CCTGTAGAAA AGGAAGAAGA TGGCACCCTG ACCATTGAAC AGCTTGACAG TCTTCGAGAT 
CAGTTCTTAG ATATGGCACC TAACGGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAGTTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCG TTCCCTCGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTC 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATAAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GCCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGGAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACAGGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ACAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808925.2
CDS	469..5358
Translation	MAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953436.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953436.2

ORF Insert Sequence:

ATGGCCAAAA TCCAAGCAGC TATTATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
AAAGCACTCG AGGCCCGAAA AATGATGAAA AAGAAAAAAG AGGCAGAAGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGATAT GGAGGAATAC AGCTGACCTC TCAGTGGACA GAAACATGGT TGGAGAGTGG 
GCCTTACCAG AAGAAATGGT TGACAATTTA CCACCCTCCA ACAATTGCAT ACTGGGCCAT 
ATTCTTCACA GGCTAGTTGA AAAATCTCTT CCTCCTCGAG CGGAATCAAC AACACCTGAA 
TTACCTTCAT TTGCTGTTAA AGGATGCTTA TTGGGGAAAA CATTAAGTGG AAAAACTACC 
ATTTTAAGGT CTCTACAAAA AGACTTTCCT ATACAGATAC TTTCTATTGA CACTCTTGTC 
CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAAAGTCA GTGAGGTTCT ACCAATTCAG 
AAAAAAGATG AAGAAGATGC TCTACCAGTT CTGCAAGAGG AGATTAAAGA AAGCCAGGAT 
CCACAACATG TATTTTCAGC TGGTCCAGTT TCAGATGAAG TATTACCAGA AACAGAAGGT 
GAAACAATAC TTAGTGGTAA TGCTGATAAA ACACCAAAAG CTGAAGAAGT CAAATCAAGT 
GATAGTTTCT TAAAACTCAC TACACGTGCT CAGCTTGGTG CAAAATCAGA ACAGTTGCTG 
AAGAAAGGAA AGAGCATTCC TGATGTGCTG CTTGTTGACA TCATGGTAAA TGCTATTAAC 
AAGATACCTG TGAATCAAGA CTGTATCCTA GATGGTTTTC CAATGACTTT AAACCAAGCA 
CAGCTTCTGG AAGAAGCTCT TACAGGCTGC AATAGAAACC TCACAGAAGT GGAAAGAAAA 
AAAGCACAAA AATCCACATT GGCTATTGAT CCTGCGACTT CCAAAGAAAT ACCTCTTCCC 
TCTCCTGCAT TTGATTTTGT CATATTATTA GATGTTTCAG ATACTTCCTC AATGAGTCGC 
ATGAATGATA TTATAGCTGA AGAATTGTCC TATAAAACTG CTCACGAAGA TATCAGTCAA 
CGTGTAGCTG CTGAAAACCA AGATAAGGAT GGAGACCAAA ATTTAAGAGA CCAGATACAA 
CATAGAATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAAAAT CAATGCTGAA ATAGATAAGG AATCTTTATG TGAAAAAGTA 
AAAGAAATTC TTACGACTGA AATAGCAAAA AAAAAGAATA AAGTTGAGAA GAAATTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCAGCT TCCCTGGCTG AGCTTCCACT TCCTACACCT 
CCTCCTGCTC CTCCTCCTGA ACCAGAAAAA GAGAAGGAAA TTCATCAAAG CCATGTGCCT 
TCAAAAACTC CTACTGCAAA AGGAAAACCT CAATCAGAAG CCCCGCATGG TAAGCAAGAA 
TCTCTTCAGG AAGGAAAAGG GAAGAAAGGT GAGACCGCAC TCAAAAGAAA AGGTTCTCCT 
AAAGGAAAAT CATCAGGAGG AAAAGTACCA GTAAAGAAAT CACCTGCTGA CTCTACAGAT 
ACATCACCTG TTGCAATAGT GCCACAGCCA CCTAAGCCAG GATCAGAAGA ATGGGTCTAT 
GTGAATGAAC CAGTTCCTGA GGAAATGCCT TCGTTTTTAG TACCTTACTG GGAACTAATA 
GAAAATTCCT ATATAAACAC CATCAAAACA GTACTCAGGC ATCTGAGGGA AGATCAGCAT 
ACTGTGCTTG CTTACTTATA TGAGATTAGA ACAAGTTTCC AGGAGTTTCT AAAGCGTCCA 
GATCACAAGC AAGATTTTGT AGCTCAATGG CAGGCTGATT TCAACTCCCT TCCTGATGAC 
CTGTGGGATG ATGAGGAAAC AAAGGCTGAA CTACATCAAC GAGTGAATGA TCTGCGAGAC 
CGCCTGTGGG ACATTTGTGA TGTCCGGAAG GAAGAGGCAG AGCAGGAGCG GCTTGACATC 
ATTAATGAGA GCTGGTTACA GGACACTCTT GGAATGACAA TGAACCATTT CTTTTCCCTG 
ATGCAGGCAG AACTGAACCG TTTCCAAGAT ACAAAGAGAC TCCTTCAAGA TTATTACTGG 
GGAATGGAAA GTAAAATCCC AGTAGAGGAC AACAAGAGAT TTACTCAAAT CCCTTTGGTC 
CAACTGGATA GTAAAGACAA TTCCGAAAGC CAGCTTAGAA TTCCTCTAGT TCCTCGAATA 
TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT GAAGAGCAAG 
ACGGATAACT CCCTAGAAAA CGTTGAGTCC AACTTTGAGG CCGATGAAAA GTTGGTCATG 
GACACCTGGC AGCAGGCTTC TTTAGCAGTA TCTCACATGG TGGCTGCTGA AATTCATCAG 
AGGCTTATGG AAGAAGAAAA AGAAAACCAG CCAGCAGACC CCAAAGAAAA ATCTCCTCAG 
ATGGGTGCAA ATAAAAAAGT CAAAAAGGAG CCACCCAAGA AAAAACAGGA AGACAAAAAA 
CCCAAAGGTA AATCATCACC TATGGCAGAA GCAACTCCTG TCATAGTAAC AACAGAGGAA 
ATTGCTGAAA TCAAAAGGAA AAATGAACTG AGGGTCAAAA TAAAAGAAGA ACACCTTGCT 
GCCTTGCAAT TTGAAGAAAT AGCCACGCAA TTTCGACTTG AACTGATAAA GACAAAAGCA 
TTGGCTCTTC TTGAAGATTT AGTAACAAAG GTGGTTGATG TATATAAACT CATGGAAAAA 
TGGCTTGGTG AGAGGTATTT GAATGAAATG GCCAGTATAG AAAAATTAAC TGACGTAGCT 
CGCTATCACA TTGAAACATC TACAAAAATT CAGAATGAAC TTTATTTAAG CCAAGAAGAC 
TTCTTCATTA ATGGCAATAT AAAAGTCTTC CCAGATCCTC CCCCACCAAT ACGTCCTCCA 
CCTGTAGAAA AGGAAGAAGA TGGCACCCTG ACCATTGAAC AGCTTGACAG TCTTCGAGAT 
CAGTTCTTAG ATATGGCACC TAACGGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAGTTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCG TTCCCTCGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTC 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATAAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GCCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGGAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACAGGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ACAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953437.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808926.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

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CloneID	OPc106452
Clone ID Related Accession (Same CDS sequence)	XM_016953437.2
Accession Version	XM_016953437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAGTTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCGTT 
CCCTCGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTCGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGGCTG GAGTGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808926.2
CDS	150..5030
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEAGVQ

Target ORF information:

RefSeq Version	XM_016953437.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953437.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAGTTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCGTT 
CCCTCGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTCGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGGCTG GAGTGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953438.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808927.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPc106453
Clone ID Related Accession (Same CDS sequence)	XM_016953438.2
Accession Version	XM_016953438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTTA TGGTTCTGAA GGTTGGAAGA TTCAAGACTG GGTATCTGGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGATAT GGAGGAATAC AGCTGACCTC TCAGTGGACA GAAACATGGT TGGAGAGTGG 
GCCTTACCAG AAGAAATGGT TGACAATTTA CCACCCTCCA ACAATTGCAT ACTGGGCCAT 
ATTCTTCACA GGCTAGTTGA AAAATCTCTT CCTCCTCGAG CGGAATCAAC AACACCTGAA 
TTACCTTCAT TTGCTGTTAA AGGATGCTTA TTGGGGAAAA CATTAAGTGG AAAAACTACC 
ATTTTAAGGT CTCTACAAAA AGACTTTCCT ATACAGATAC TTTCTATTGA CACTCTTGTC 
CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAAAGTCA GTGAGGTTCT ACCAATTCAG 
AAAAAAGATG AAGAAGATGC TCTACCAGTT CTGCAAGAGG AGATTAAAGA AAGCCAGGAT 
CCACAACATG TATTTTCAGC TGGTCCAGTT TCAGATGAAG TATTACCAGA AACAGAAGGT 
GAAACAATAC TTAGTGGTAA TGCTGATAAA ACACCAAAAG CTGAAGAAGT CAAATCAAGT 
GATAGTTTCT TAAAACTCAC TACACGTGCT CAGCTTGGTG CAAAATCAGA ACAGTTGCTG 
AAGAAAGGAA AGAGCATTCC TGATGTGCTG CTTGTTGACA TCATGGTAAA TGCTATTAAC 
AAGATACCTG TGAATCAAGA CTGTATCCTA GATGGTTTTC CAATGACTTT AAACCAAGCA 
CAGCTTCTGG AAGAAGCTCT TACAGGCTGC AATAGAAACC TCACAGAAGT GGAAAGAAAA 
AAAGCACAAA AATCCACATT GGCTATTGAT CCTGCGACTT CCAAAGAAAT ACCTCTTCCC 
TCTCCTGCAT TTGATTTTGT CATATTATTA GATGTTTCAG ATACTTCCTC AATGAGTCGC 
ATGAATGATA TTATAGCTGA AGAATTGTCC TATAAAACTG CTCACGAAGA TATCAGTCAA 
CGTGTAGCTG CTGAAAACCA AGATAAGGAT GGAGACCAAA ATTTAAGAGA CCAGATACAA 
CATAGAATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAAAAT CAATGCTGAA ATAGATAAGG AATCTTTATG TGAAAAAGTA 
AAAGAAATTC TTACGACTGA AATAGCAAAA AAAAAGAATA AAGTTGAGAA GAAATTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCAGCT TCCCTGGCTG AGCTTCCACT TCCTACACCT 
CCTCCTGCTC CTCCTCCTGA ACCAGAAAAA GAGAAGGAAA TTCATCAAAG CCATGTGCCT 
TCAAAAACTC CTACTGCAAA AGGAAAACCT CAATCAGAAG CCCCGCATGG TAAGCAAGAA 
TCTCTTCAGG AAGGAAAAGG GAAGAAAGGT GAGACCGCAC TCAAAAGAAA AGGTTCTCCT 
AAAGGAAAAT CATCAGGAGG AAAAGTACCA GTAAAGAAAT CACCTGCTGA CTCTACAGAT 
ACATCACCTG TTGCAATAGT GCCACAGCCA CCTAAGCCAG GATCAGAAGA ATGGGTCTAT 
GTGAATGAAC CAGTTCCTGA GGAAATGCCT TCGTTTTTAG TACCTTACTG GGAACTAATA 
GAAAATTCCT ATATAAACAC CATCAAAACA GTACTCAGGC ATCTGAGGGA AGATCAGCAT 
ACTGTGCTTG CTTACTTATA TGAGATTAGA ACAAGTTTCC AGGAGTTTCT AAAGCGTCCA 
GATCACAAGC AAGATTTTGT AGCTCAATGG CAGGCTGATT TCAACTCCCT TCCTGATGAC 
CTGTGGGATG ATGAGGAAAC AAAGGCTGAA CTACATCAAC GAGTGAATGA TCTGCGAGAC 
CGCCTGTGGG ACATTTGTGA TGTCCGGAAG GAAGAGGCAG AGCAGGAGCG GCTTGACATC 
ATTAATGAGA GCTGGTTACA GGACACTCTT GGAATGACAA TGAACCATTT CTTTTCCCTG 
ATGCAGGCAG AACTGAACCG TTTCCAAGAT ACAAAGAGAC TCCTTCAAGA TTATTACTGG 
GGAATGGAAA GTAAAATCCC AGTAGAGGAC AACAAGAGAT TTACTCAAAT CCCTTTGGTC 
CAACTGGATA GTAAAGACAA TTCCGAAAGC CAGCTTAGAA TTCCTCTAGT TCCTCGAATA 
TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT GAAGAGCAAG 
ACGGATAACT CCCTAGAAAA CGTTGAGTCC AACTTTGAGG CCGATGAAAA GTTGGTCATG 
GACACCTGGC AGCAGGCTTC TTTAGCAGTA TCTCACATGG TGGCTGCTGA AATTCATCAG 
AGGCTTATGG AAGAAGAAAA AGAAAACCAG CCAGCAGACC CCAAAGAAAA ATCTCCTCAG 
ATGGGTGCAA ATAAAAAAGT CAAAAAGGAG CCACCCAAGA AAAAACAGGA AGACAAAAAA 
CCCAAAGGTA AATCATCACC TATGGCAGAA GCAACTCCTG TCATAGTAAC AACAGAGGAA 
ATTGCTGAAA TCAAAAGGAA AAATGAACTG AGGGTCAAAA TAAAAGAAGA ACACCTTGCT 
GCCTTGCAAT TTGAAGAAAT AGCCACGCAA TTTCGACTTG AACTGATAAA GACAAAAGCA 
TTGGCTCTTC TTGAAGATTT AGTAACAAAG GTGGTTGATG TATATAAACT CATGGAAAAA 
TGGCTTGGTG AGAGGTATTT GAATGAAATG GCCAGTATAG AAAAATTAAC TGACGTAGCT 
CGCTATCACA TTGAAACATC TACAAAAATT CAGAATGAAC TTTATTTAAG CCAAGAAGAC 
TTCTTCATTA ATGGCAATAT AAAAGTCTTC CCAGATCCTC CCCCACCAAT ACGTCCTCCA 
CCTGTAGAAA AGGAAGAAGA TGGCACCCTG ACCATTGAAC AGCTTGACAG TCTTCGAGAT 
CAGTTCTTAG ATATGGCACC TAACGGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAGTTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCG TTCCCTCGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTC 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATAAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GCCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGGAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACAGGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ACAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808927.2
CDS	357..5186
Translation	MSLMVLKVGRFKTGYLDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953438.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953438.2

ORF Insert Sequence:

ATGTCACTTA TGGTTCTGAA GGTTGGAAGA TTCAAGACTG GGTATCTGGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGATAT GGAGGAATAC AGCTGACCTC TCAGTGGACA GAAACATGGT TGGAGAGTGG 
GCCTTACCAG AAGAAATGGT TGACAATTTA CCACCCTCCA ACAATTGCAT ACTGGGCCAT 
ATTCTTCACA GGCTAGTTGA AAAATCTCTT CCTCCTCGAG CGGAATCAAC AACACCTGAA 
TTACCTTCAT TTGCTGTTAA AGGATGCTTA TTGGGGAAAA CATTAAGTGG AAAAACTACC 
ATTTTAAGGT CTCTACAAAA AGACTTTCCT ATACAGATAC TTTCTATTGA CACTCTTGTC 
CAAGAAGCTA TCCAAGCATT TCATGACAAT GAAAAAGTCA GTGAGGTTCT ACCAATTCAG 
AAAAAAGATG AAGAAGATGC TCTACCAGTT CTGCAAGAGG AGATTAAAGA AAGCCAGGAT 
CCACAACATG TATTTTCAGC TGGTCCAGTT TCAGATGAAG TATTACCAGA AACAGAAGGT 
GAAACAATAC TTAGTGGTAA TGCTGATAAA ACACCAAAAG CTGAAGAAGT CAAATCAAGT 
GATAGTTTCT TAAAACTCAC TACACGTGCT CAGCTTGGTG CAAAATCAGA ACAGTTGCTG 
AAGAAAGGAA AGAGCATTCC TGATGTGCTG CTTGTTGACA TCATGGTAAA TGCTATTAAC 
AAGATACCTG TGAATCAAGA CTGTATCCTA GATGGTTTTC CAATGACTTT AAACCAAGCA 
CAGCTTCTGG AAGAAGCTCT TACAGGCTGC AATAGAAACC TCACAGAAGT GGAAAGAAAA 
AAAGCACAAA AATCCACATT GGCTATTGAT CCTGCGACTT CCAAAGAAAT ACCTCTTCCC 
TCTCCTGCAT TTGATTTTGT CATATTATTA GATGTTTCAG ATACTTCCTC AATGAGTCGC 
ATGAATGATA TTATAGCTGA AGAATTGTCC TATAAAACTG CTCACGAAGA TATCAGTCAA 
CGTGTAGCTG CTGAAAACCA AGATAAGGAT GGAGACCAAA ATTTAAGAGA CCAGATACAA 
CATAGAATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAAAAT CAATGCTGAA ATAGATAAGG AATCTTTATG TGAAAAAGTA 
AAAGAAATTC TTACGACTGA AATAGCAAAA AAAAAGAATA AAGTTGAGAA GAAATTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCAGCT TCCCTGGCTG AGCTTCCACT TCCTACACCT 
CCTCCTGCTC CTCCTCCTGA ACCAGAAAAA GAGAAGGAAA TTCATCAAAG CCATGTGCCT 
TCAAAAACTC CTACTGCAAA AGGAAAACCT CAATCAGAAG CCCCGCATGG TAAGCAAGAA 
TCTCTTCAGG AAGGAAAAGG GAAGAAAGGT GAGACCGCAC TCAAAAGAAA AGGTTCTCCT 
AAAGGAAAAT CATCAGGAGG AAAAGTACCA GTAAAGAAAT CACCTGCTGA CTCTACAGAT 
ACATCACCTG TTGCAATAGT GCCACAGCCA CCTAAGCCAG GATCAGAAGA ATGGGTCTAT 
GTGAATGAAC CAGTTCCTGA GGAAATGCCT TCGTTTTTAG TACCTTACTG GGAACTAATA 
GAAAATTCCT ATATAAACAC CATCAAAACA GTACTCAGGC ATCTGAGGGA AGATCAGCAT 
ACTGTGCTTG CTTACTTATA TGAGATTAGA ACAAGTTTCC AGGAGTTTCT AAAGCGTCCA 
GATCACAAGC AAGATTTTGT AGCTCAATGG CAGGCTGATT TCAACTCCCT TCCTGATGAC 
CTGTGGGATG ATGAGGAAAC AAAGGCTGAA CTACATCAAC GAGTGAATGA TCTGCGAGAC 
CGCCTGTGGG ACATTTGTGA TGTCCGGAAG GAAGAGGCAG AGCAGGAGCG GCTTGACATC 
ATTAATGAGA GCTGGTTACA GGACACTCTT GGAATGACAA TGAACCATTT CTTTTCCCTG 
ATGCAGGCAG AACTGAACCG TTTCCAAGAT ACAAAGAGAC TCCTTCAAGA TTATTACTGG 
GGAATGGAAA GTAAAATCCC AGTAGAGGAC AACAAGAGAT TTACTCAAAT CCCTTTGGTC 
CAACTGGATA GTAAAGACAA TTCCGAAAGC CAGCTTAGAA TTCCTCTAGT TCCTCGAATA 
TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT GAAGAGCAAG 
ACGGATAACT CCCTAGAAAA CGTTGAGTCC AACTTTGAGG CCGATGAAAA GTTGGTCATG 
GACACCTGGC AGCAGGCTTC TTTAGCAGTA TCTCACATGG TGGCTGCTGA AATTCATCAG 
AGGCTTATGG AAGAAGAAAA AGAAAACCAG CCAGCAGACC CCAAAGAAAA ATCTCCTCAG 
ATGGGTGCAA ATAAAAAAGT CAAAAAGGAG CCACCCAAGA AAAAACAGGA AGACAAAAAA 
CCCAAAGGTA AATCATCACC TATGGCAGAA GCAACTCCTG TCATAGTAAC AACAGAGGAA 
ATTGCTGAAA TCAAAAGGAA AAATGAACTG AGGGTCAAAA TAAAAGAAGA ACACCTTGCT 
GCCTTGCAAT TTGAAGAAAT AGCCACGCAA TTTCGACTTG AACTGATAAA GACAAAAGCA 
TTGGCTCTTC TTGAAGATTT AGTAACAAAG GTGGTTGATG TATATAAACT CATGGAAAAA 
TGGCTTGGTG AGAGGTATTT GAATGAAATG GCCAGTATAG AAAAATTAAC TGACGTAGCT 
CGCTATCACA TTGAAACATC TACAAAAATT CAGAATGAAC TTTATTTAAG CCAAGAAGAC 
TTCTTCATTA ATGGCAATAT AAAAGTCTTC CCAGATCCTC CCCCACCAAT ACGTCCTCCA 
CCTGTAGAAA AGGAAGAAGA TGGCACCCTG ACCATTGAAC AGCTTGACAG TCTTCGAGAT 
CAGTTCTTAG ATATGGCACC TAACGGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAGTTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCG TTCCCTCGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTC 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATAAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GCCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGGAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACAGGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ACAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953439.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808928.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OPc106454
Clone ID Related Accession (Same CDS sequence)	XM_016953439.2
Accession Version	XM_016953439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036884.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016953439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGAGTGCTC TGAGAACTGC 
TTTCAAAGGC CTTCACCTTC GACCTGTGAT GCCTTCTCAA ATAGGAGACA GGAAGTGGCT 
TCATGGATTA CAGGCAGCCC TCCAGAACTT TCTCAAGGGA TATCCTGCAA TTGTTCGGCA 
ACTGCACTCA GCAGGCAGAG GCAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808928.2
CDS	150..4799
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKIWRNTADLSVDRNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNECSENCFQRPSPSTCDAFSNRRQEVASWITGSPPELSQGISCNCSATALSRQRQK

Target ORF information:

RefSeq Version	XM_016953439.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953439.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGATATGG AGGAATACAG CTGACCTCTC AGTGGACAGA 
AACATGGTTG GAGAGTGGGC CTTACCAGAA GAAATGGTTG ACAATTTACC ACCCTCCAAC 
AATTGCATAC TGGGCCATAT TCTTCACAGG CTAGTTGAAA AATCTCTTCC TCCTCGAGCG 
GAATCAACAA CACCTGAATT ACCTTCATTT GCTGTTAAAG GATGCTTATT GGGGAAAACA 
TTAAGTGGAA AAACTACCAT TTTAAGGTCT CTACAAAAAG ACTTTCCTAT ACAGATACTT 
TCTATTGACA CTCTTGTCCA AGAAGCTATC CAAGCATTTC ATGACAATGA AAAAGTCAGT 
GAGGTTCTAC CAATTCAGAA AAAAGATGAA GAAGATGCTC TACCAGTTCT GCAAGAGGAG 
ATTAAAGAAA GCCAGGATCC ACAACATGTA TTTTCAGCTG GTCCAGTTTC AGATGAAGTA 
TTACCAGAAA CAGAAGGTGA AACAATACTT AGTGGTAATG CTGATAAAAC ACCAAAAGCT 
GAAGAAGTCA AATCAAGTGA TAGTTTCTTA AAACTCACTA CACGTGCTCA GCTTGGTGCA 
AAATCAGAAC AGTTGCTGAA GAAAGGAAAG AGCATTCCTG ATGTGCTGCT TGTTGACATC 
ATGGTAAATG CTATTAACAA GATACCTGTG AATCAAGACT GTATCCTAGA TGGTTTTCCA 
ATGACTTTAA ACCAAGCACA GCTTCTGGAA GAAGCTCTTA CAGGCTGCAA TAGAAACCTC 
ACAGAAGTGG AAAGAAAAAA AGCACAAAAA TCCACATTGG CTATTGATCC TGCGACTTCC 
AAAGAAATAC CTCTTCCCTC TCCTGCATTT GATTTTGTCA TATTATTAGA TGTTTCAGAT 
ACTTCCTCAA TGAGTCGCAT GAATGATATT ATAGCTGAAG AATTGTCCTA TAAAACTGCT 
CACGAAGATA TCAGTCAACG TGTAGCTGCT GAAAACCAAG ATAAGGATGG AGACCAAAAT 
TTAAGAGACC AGATACAACA TAGAATTATA GGCTTCTTGG ACAACTGGCC TTTATTGGAG 
CAATGGTTTT CAGAGCCAGA AAATATTTTG ATAAAAATCA ATGCTGAAAT AGATAAGGAA 
TCTTTATGTG AAAAAGTAAA AGAAATTCTT ACGACTGAAA TAGCAAAAAA AAAGAATAAA 
GTTGAGAAGA AATTAGAAGA AAAGGAAGCT GAGAAAAAAG CAGCAGCTTC CCTGGCTGAG 
CTTCCACTTC CTACACCTCC TCCTGCTCCT CCTCCTGAAC CAGAAAAAGA GAAGGAAATT 
CATCAAAGCC ATGTGCCTTC AAAAACTCCT ACTGCAAAAG GAAAACCTCA ATCAGAAGCC 
CCGCATGGTA AGCAAGAATC TCTTCAGGAA GGAAAAGGGA AGAAAGGTGA GACCGCACTC 
AAAAGAAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTC GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ATCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG TCCGGAAGGA AGAGGCAGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCAAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CCGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGAGCAAGAC GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCATCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTATAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCACCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GCACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA ACGAGTGCTC TGAGAACTGC 
TTTCAAAGGC CTTCACCTTC GACCTGTGAT GCCTTCTCAA ATAGGAGACA GGAAGTGGCT 
TCATGGATTA CAGGCAGCCC TCCAGAACTT TCTCAAGGGA TATCCTGCAA TTGTTCGGCA 
ACTGCACTCA GCAGGCAGAG GCAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953430.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808919.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

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CloneID	OPc85070
Clone ID Related Accession (Same CDS sequence)	XM_016953430.1
Accession Version	XM_016953430.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCACTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808919.1
CDS	150..5621
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953430.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953430.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCACTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_009449294.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009447569.2 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OPc85071
Clone ID Related Accession (Same CDS sequence)	XM_009449294.2
Accession Version	XM_009449294.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009449294.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCACTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009447569.2
CDS	150..5618
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_009449294.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009449294.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATAAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGCCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGG AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACAGG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAACAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCACTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953431.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808920.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OPc85072
Clone ID Related Accession (Same CDS sequence)	XM_016953431.1
Accession Version	XM_016953431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGGTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACAAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATAAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCAC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808920.1
CDS	150..5606
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953431.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953431.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGGTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TGGTAAATGC TATTAACAAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGCCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATAAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCAC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953432.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808921.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

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CloneID	OPc85073
Clone ID Related Accession (Same CDS sequence)	XM_016953432.1
Accession Version	XM_016953432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGCT GGAGAATGCC AAGGCGTAGC TGTCCCAAGT CATTTGCAAA GGCATTTTCC 
AGTGGCTATC TACTTGGAGA AGTTCTACAC AAGTTTGAAC TTCAGGATGA TTTTTCAGAA 
TTTTTGGACA GCAGGGTTTC AAGTGCCAAA CTTAATAATT TTTCTCGCTT GGAGCCAACA 
CTTCACCTTC TGGGTGTGCA GTTTGATCAG AATGTGGCCC ATGGCATCAT CACAGAAAAG 
CCTGGGGTGG CAACAAAGCT GTTATATCAA TTGTACATTG CTCTTCAGAA AAAGAAGAAA 
AGTGGACTGA CTGGAGTGGA GATGCAAACC ATGCAACGTC TGACAAATTT AAGACTTCAA 
AACTTGAAAA GTGATACTTT TCAAGAGAGA CTTAGACACA TGATACCACG TCAAACTGAT 
TTCAATCTGA TGCGGATTAC ATACAGGTTT CAAGAAAAAT ATAAACACGT GAAAGAAGAT 
CTTGCCCATT TGCATTTTGA GAAACTTGAA AGATTTCAAA AACTCAAGGA AGAGCAAAGA 
TGTTTTGATA TCGAAAAGCA ATACTTAAAC AGAAGACGAC AAAATGAAAT AATGGCCAAA 
ATCCAAGCAG CTATTATACA GATTCCTAAA CCTGCATCAA ATCGTACTTT GAAAGCACTC 
GAGGCCCGAA AAATGATGAA AAAGAAAAAA GAGGCAGAAG ATGTGGCTGA TGAAATTAAG 
AAGTTCGAAG CATTAATAAA AAAGGATCTC CAAGCAAAAG AAAGTGCATC CAAGACTTCT 
TTAGATACAG CAGGCCAGAC AACCACCGAT TTGTTAAATA CTTACTCAGA TGACGAGTAC 
ATTAAAAAAA TCCAGAAGCG CCTTGAAGAA GATGCTTTTG CACGAGAGCA AAGGGAGAAA 
AGACGGCGGA AATTGTTAAT GGACCAGTTA ATAGCCCACG AAGCACAAGA GGAGGCTTAT 
CGGGAGGAAC AGCTGATTAA CCGGCTGATG CGGCAGTCCC AGCAGGAGCG CAGGATTGCC 
GTGCAGCTCA TGCATGTTCG GCATGAAAAG GAAGTTTTAT GGCAAAACAG AATTTTCAGA 
GAAAAACAAC ATGAGGAAAG ACGACTTAAA GATTTCCAGG ATGCTCTTGA TCGAGAAGCG 
GCTTTGGCAA AACAAGCCAA GATTGACTTT GAAGAACAAT TCCTTAAAGA AAAGAGATTT 
CATGATCAGA TTGCTGTGGA AAGAGCTCAA GCTCGTTATG AAAAGCATTA TTCAGTATGT 
GCAGAAATTT TGGATCAAAT AGTTGATTTG TCCACTAAAG TGGCAGACTA TCGAATGTTG 
ACAAATAATC TGATTCCGTA TAAGTTGATG CATGATTGGA AGGAACTATT TTTTAATGCA 
AAACCCATAT ATGAACAAGC CTCTGTTAAG ACACTACCTG CTGACCCCTC AAGAGAACAA 
CTTACAGAAC TGGAGAAAAG GGACTTGCTA GATACCAATG ATTATGAAGA ATATAAGAAC 
ATGGTTGGAG AGTGGGCCTT ACCAGAAGAA ATGGTTGACA ATTTACCACC CTCCAACAAT 
TGCATACTGG GCCATATTCT TCACAGGCTA GTTGAAAAAT CTCTTCCTCC TCGAGCGGAA 
TCAACAACAC CTGAATTACC TTCATTTGCT GTTAAAGGAT GCTTATTGGG GAAAACATTA 
AGTGGAAAAA CTACCATTTT AAGGTCTCTA CAAAAAGACT TTCCTATACA GATACTTTCT 
ATTGACACTC TTGTCCAAGA AGCTATCCAA GCATTTCATG ACAATGAAAA AGTCAGTGAG 
GTTCTACCAA TTCAGAAAAA AGATGAAGAA GATGCTCTAC CAGTTCTGCA AGAGGAGATT 
AAAGAAAGCC AGGATCCACA ACATGTATTT TCAGCTGGTC CAGTTTCAGA TGAAGTATTA 
CCAGAAACAG AAGGTGAAAC AATACTTAGT GGTAATGCTG ATAAAACACC AAAAGCTGAA 
GAAGTCAAAT CAAGTGATAG TTTCTTAAAA CTCACTACAC GTGCTCAGCT TGGTGCAAAA 
TCAGAACAGT TGCTGAAGAA AGGAAAGAGC ATTCCTGATG TGCTGCTTGT TGACATCATG 
GTAAATGCTA TTAACAAGAT ACCTGTGAAT CAAGACTGTA TCCTAGATGG TTTTCCAATG 
ACTTTAAACC AAGCACAGCT TCTGGAAGAA GCTCTTACAG GCTGCAATAG AAACCTCACA 
GAAGTGGAAA GAAAAAAAGC ACAAAAATCC ACATTGGCTA TTGATCCTGC GACTTCCAAA 
GAAATACCTC TTCCCTCTCC TGCATTTGAT TTTGTCATAT TATTAGATGT TTCAGATACT 
TCCTCAATGA GTCGCATGAA TGATATTATA GCTGAAGAAT TGTCCTATAA AACTGCTCAC 
GAAGATATCA GTCAACGTGT AGCTGCTGAA AACCAAGATA AGGATGGAGA CCAAAATTTA 
AGAGACCAGA TACAACATAG AATTATAGGC TTCTTGGACA ACTGGCCTTT ATTGGAGCAA 
TGGTTTTCAG AGCCAGAAAA TATTTTGATA AAAATCAATG CTGAAATAGA TAAGGAATCT 
TTATGTGAAA AAGTAAAAGA AATTCTTACG ACTGAAATAG CAAAAAAAAA GAATAAAGTT 
GAGAAGAAAT TAGAAGAAAA GGAAGCTGAG AAAAAAGCAG CAGCTTCCCT GGCTGAGCTT 
CCACTTCCTA CACCTCCTCC TGCTCCTCCT CCTGAACCAG AAAAAGAGAA GGAAATTCAT 
CAAAGCCATG TGCCTTCAAA AACTCCTACT GCAAAAGGAA AACCTCAATC AGAAGCCCCG 
CATGGTAAGC AAGAATCTCT TCAGGAAGGA AAAGGGAAGA AAGGTGAGAC CGCACTCAAA 
AGAAAAGGTT CTCCTAAAGG AAAATCATCA GGAGGAAAAG TACCAGTAAA GAAATCACCT 
GCTGACTCTA CAGATACATC ACCTGTTGCA ATAGTGCCAC AGCCACCTAA GCCAGGATCA 
GAAGAATGGG TCTATGTGAA TGAACCAGTT CCTGAGGAAA TGCCTTCGTT TTTAGTACCT 
TACTGGGAAC TAATAGAAAA TTCCTATATA AACACCATCA AAACAGTACT CAGGCATCTG 
AGGGAAGATC AGCATACTGT GCTTGCTTAC TTATATGAGA TTAGAACAAG TTTCCAGGAG 
TTTCTAAAGC GTCCAGATCA CAAGCAAGAT TTTGTAGCTC AATGGCAGGC TGATTTCAAC 
TCCCTTCCTG ATGACCTGTG GGATGATGAG GAAACAAAGG CTGAACTACA TCAACGAGTG 
AATGATCTGC GAGACCGCCT GTGGGACATT TGTGATGTCC GGAAGGAAGA GGCAGAGCAG 
GAGCGGCTTG ACATCATTAA TAAGAGCTGG TTACAGGACA CTCTTGGAAT GACAATGAAC 
CATTTCTTTT CCCTGATGCA GGCAGAACTG AACCGTTTCC AAGATACAAA GAGACTCCTT 
CAAGATTATT ACTGGGGAAT GGAAAGTAAA ATCCCAGTAG AGGACAACAA GAGATTTACT 
CAAATCCCTT TGGTCCAACT GGATAGTAAA GACAATTCCG AAAGCCAGCT TAGAATTCCT 
CTAGTTCCTC GAATATCCAT TTCTCTGGAA ACAGTTACAC CCAAACCAAA AACAAAATCA 
GTACTGAAGA GCAAGACGGA TAACTCCCTA GAAAACGTTG AGTCCAACTT TGAGGCCGAT 
GAAAAGTTGG TCATGGACAC CTGGCAGCAG GCTTCTTTAG CAGTATCTCA CATGGTGGCT 
GCTGAAATTC ATCAGAGGCT TATGGAAGAA GAAAAAGAAA ACCAGCCAGC AGACCCCAAA 
GAAAAATCTC CTCAGATGGG TGCAAATAAA AAAGTCAAAA AGGAGCCACC CAAGAAAAAA 
CAGGAAGACA AAAAACCCAA AGGTAAATCA TCACCTATGG CAGAAGCAAC TCCTGTCATA 
GTAACAACAG AGGAAATTGC TGAAATCAAA AGGAAAAATG AACTGAGGGT CAAAATAAAA 
GAAGAACACC TTGCTGCCTT GCAATTTGAA GAAATAGCCA CGCAATTTCG ACTTGAACTG 
ATAAAGACAA AAGCATTGGC TCTTCTTGAA GATTTAGTAA CAAAGGTGGT TGATGTATAT 
AAACTCATGG AAAAATGGCT TGGTGAGAGG TATTTGAATG AAATGGCCAG TATAGAAAAA 
TTAACTGACG TAGCTCGCTA TCACATTGAA ACATCTACAA AAATTCAGAA TGAACTTTAT 
TTAAGCCAAG AAGACTTCTT CATTAATGGC AATATAAAAG TCTTCCCAGA TCCTCCCCCA 
CCAATACGTC CTCCACCTGT AGAAAAGGAA GAAGATGGCA CCCTGACCAT TGAACAGCTT 
GACAGTCTTC GAGATCAGTT CTTAGATATG GCACCTAACG GCATAATAGG AAATAAAGCA 
TTTACTGACA TTCTGATCGA TTTGGTGACC CTGAACCTTG GCACAAACAA CTTTCCTAGT 
AGTTGGATGC ACCTTACCCA ACCTGAATTA CAGGAATTAA CATCTTTATT AACAGTCAAC 
TCCGAGTTCG TGGACTGGCG GAAGTTCCTG TTAGTAACCT CAATGCCTTG GCCCGTTCCC 
TCGGAGGAGG AGCTCCTTGA GACCCTTCAG AAGTTCAAGG CTGTGGATAA GGAGCAGTTG 
GGCACCATCA CTTTCGAGCA GTATATGCAG GCTGGTCTGT GGTTTACAGG AGATGAAGAT 
ATAAAAATTC CAGAAAATCC TCTTGAACCC CTTCCATTTA ATAGGCAGGA GCATCTTATA 
GAGTTTTTCT TTAGGCTATT TGCTGACTAT AAGAAGGATC CACCCCAGCT TGACTACACA 
CAGATGCTGC TTTACTTTGC TTGCCACCCA GACACCGTGG AAGGAGCCTA CAGGGCCCTC 
AGTGTGGCTG TTGGAACTCA TGTCTTCCAA CAAGTCAAAG CTTCCATTCC AAGTGCAGAA 
AAGACCTCCT CAACTGATGC AGGTCCAGCT GAGGAATTTC CTGAACCTGA GGGAAATGCT 
GCAAGAGAAG AAAGGAAATT AAAAGACGAC AGGGAGAAAA GGGAACAGAA GGATGAAGAA 
ATCCCTGAAA ATGCAAACAA TGAAAAGATG TCCATGGAAA CACTACTCAA AGTGTTCAAA 
GGGGGAAGTG AAGCACAGGA CTCCAACAGA TTTGCCAGCC ACCTAAAGAT AGAGAACATT 
TATGCAGAGG GCTTCATAAA AACATTTCAA GACCTAGGTG CCAAGAACCT GGAGCCAATT 
GAAGTCACTG TTCTCTTGAA GCATCCTTTT ATTCAAGACC TGATTTCAAA TTATTCAGAC 
TATAAGTTTC CTGTACACAT CATCTATGTA GTGGAAATGA TAGTGTGTGC AAAAGGTTGG 
GCACCACTTC CATCAGGAAA ACAGTGTGCA AGATGGAGAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808921.1
CDS	68..5512
Translation	MFSWRMPRRSCPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953432.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953432.1

ORF Insert Sequence:

ATGTTCAGCT GGAGAATGCC AAGGCGTAGC TGTCCCAAGT CATTTGCAAA GGCATTTTCC 
AGTGGCTATC TACTTGGAGA AGTTCTACAC AAGTTTGAAC TTCAGGATGA TTTTTCAGAA 
TTTTTGGACA GCAGGGTTTC AAGTGCCAAA CTTAATAATT TTTCTCGCTT GGAGCCAACA 
CTTCACCTTC TGGGTGTGCA GTTTGATCAG AATGTGGCCC ATGGCATCAT CACAGAAAAG 
CCTGGGGTGG CAACAAAGCT GTTATATCAA TTGTACATTG CTCTTCAGAA AAAGAAGAAA 
AGTGGACTGA CTGGAGTGGA GATGCAAACC ATGCAACGTC TGACAAATTT AAGACTTCAA 
AACTTGAAAA GTGATACTTT TCAAGAGAGA CTTAGACACA TGATACCACG TCAAACTGAT 
TTCAATCTGA TGCGGATTAC ATACAGGTTT CAAGAAAAAT ATAAACACGT GAAAGAAGAT 
CTTGCCCATT TGCATTTTGA GAAACTTGAA AGATTTCAAA AACTCAAGGA AGAGCAAAGA 
TGTTTTGATA TCGAAAAGCA ATACTTAAAC AGAAGACGAC AAAATGAAAT AATGGCCAAA 
ATCCAAGCAG CTATTATACA GATTCCTAAA CCTGCATCAA ATCGTACTTT GAAAGCACTC 
GAGGCCCGAA AAATGATGAA AAAGAAAAAA GAGGCAGAAG ATGTGGCTGA TGAAATTAAG 
AAGTTCGAAG CATTAATAAA AAAGGATCTC CAAGCAAAAG AAAGTGCATC CAAGACTTCT 
TTAGATACAG CAGGCCAGAC AACCACCGAT TTGTTAAATA CTTACTCAGA TGACGAGTAC 
ATTAAAAAAA TCCAGAAGCG CCTTGAAGAA GATGCTTTTG CACGAGAGCA AAGGGAGAAA 
AGACGGCGGA AATTGTTAAT GGACCAGTTA ATAGCCCACG AAGCACAAGA GGAGGCTTAT 
CGGGAGGAAC AGCTGATTAA CCGGCTGATG CGGCAGTCCC AGCAGGAGCG CAGGATTGCC 
GTGCAGCTCA TGCATGTTCG GCATGAAAAG GAAGTTTTAT GGCAAAACAG AATTTTCAGA 
GAAAAACAAC ATGAGGAAAG ACGACTTAAA GATTTCCAGG ATGCTCTTGA TCGAGAAGCG 
GCTTTGGCAA AACAAGCCAA GATTGACTTT GAAGAACAAT TCCTTAAAGA AAAGAGATTT 
CATGATCAGA TTGCTGTGGA AAGAGCTCAA GCTCGTTATG AAAAGCATTA TTCAGTATGT 
GCAGAAATTT TGGATCAAAT AGTTGATTTG TCCACTAAAG TGGCAGACTA TCGAATGTTG 
ACAAATAATC TGATTCCGTA TAAGTTGATG CATGATTGGA AGGAACTATT TTTTAATGCA 
AAACCCATAT ATGAACAAGC CTCTGTTAAG ACACTACCTG CTGACCCCTC AAGAGAACAA 
CTTACAGAAC TGGAGAAAAG GGACTTGCTA GATACCAATG ATTATGAAGA ATATAAGAAC 
ATGGTTGGAG AGTGGGCCTT ACCAGAAGAA ATGGTTGACA ATTTACCACC CTCCAACAAT 
TGCATACTGG GCCATATTCT TCACAGGCTA GTTGAAAAAT CTCTTCCTCC TCGAGCGGAA 
TCAACAACAC CTGAATTACC TTCATTTGCT GTTAAAGGAT GCTTATTGGG GAAAACATTA 
AGTGGAAAAA CTACCATTTT AAGGTCTCTA CAAAAAGACT TTCCTATACA GATACTTTCT 
ATTGACACTC TTGTCCAAGA AGCTATCCAA GCATTTCATG ACAATGAAAA AGTCAGTGAG 
GTTCTACCAA TTCAGAAAAA AGATGAAGAA GATGCTCTAC CAGTTCTGCA AGAGGAGATT 
AAAGAAAGCC AGGATCCACA ACATGTATTT TCAGCTGGTC CAGTTTCAGA TGAAGTATTA 
CCAGAAACAG AAGGTGAAAC AATACTTAGT GGTAATGCTG ATAAAACACC AAAAGCTGAA 
GAAGTCAAAT CAAGTGATAG TTTCTTAAAA CTCACTACAC GTGCTCAGCT TGGTGCAAAA 
TCAGAACAGT TGCTGAAGAA AGGAAAGAGC ATTCCTGATG TGCTGCTTGT TGACATCATG 
GTAAATGCTA TTAACAAGAT ACCTGTGAAT CAAGACTGTA TCCTAGATGG TTTTCCAATG 
ACTTTAAACC AAGCACAGCT TCTGGAAGAA GCTCTTACAG GCTGCAATAG AAACCTCACA 
GAAGTGGAAA GAAAAAAAGC ACAAAAATCC ACATTGGCTA TTGATCCTGC GACTTCCAAA 
GAAATACCTC TTCCCTCTCC TGCATTTGAT TTTGTCATAT TATTAGATGT TTCAGATACT 
TCCTCAATGA GTCGCATGAA TGATATTATA GCTGAAGAAT TGTCCTATAA AACTGCTCAC 
GAAGATATCA GTCAACGTGT AGCTGCTGAA AACCAAGATA AGGATGGAGA CCAAAATTTA 
AGAGACCAGA TACAACATAG AATTATAGGC TTCTTGGACA ACTGGCCTTT ATTGGAGCAA 
TGGTTTTCAG AGCCAGAAAA TATTTTGATA AAAATCAATG CTGAAATAGA TAAGGAATCT 
TTATGTGAAA AAGTAAAAGA AATTCTTACG ACTGAAATAG CAAAAAAAAA GAATAAAGTT 
GAGAAGAAAT TAGAAGAAAA GGAAGCTGAG AAAAAAGCAG CAGCTTCCCT GGCTGAGCTT 
CCACTTCCTA CACCTCCTCC TGCTCCTCCT CCTGAACCAG AAAAAGAGAA GGAAATTCAT 
CAAAGCCATG TGCCTTCAAA AACTCCTACT GCAAAAGGAA AACCTCAATC AGAAGCCCCG 
CATGGTAAGC AAGAATCTCT TCAGGAAGGA AAAGGGAAGA AAGGTGAGAC CGCACTCAAA 
AGAAAAGGTT CTCCTAAAGG AAAATCATCA GGAGGAAAAG TACCAGTAAA GAAATCACCT 
GCTGACTCTA CAGATACATC ACCTGTTGCA ATAGTGCCAC AGCCACCTAA GCCAGGATCA 
GAAGAATGGG TCTATGTGAA TGAACCAGTT CCTGAGGAAA TGCCTTCGTT TTTAGTACCT 
TACTGGGAAC TAATAGAAAA TTCCTATATA AACACCATCA AAACAGTACT CAGGCATCTG 
AGGGAAGATC AGCATACTGT GCTTGCTTAC TTATATGAGA TTAGAACAAG TTTCCAGGAG 
TTTCTAAAGC GTCCAGATCA CAAGCAAGAT TTTGTAGCTC AATGGCAGGC TGATTTCAAC 
TCCCTTCCTG ATGACCTGTG GGATGATGAG GAAACAAAGG CTGAACTACA TCAACGAGTG 
AATGATCTGC GAGACCGCCT GTGGGACATT TGTGATGTCC GGAAGGAAGA GGCAGAGCAG 
GAGCGGCTTG ACATCATTAA TAAGAGCTGG TTACAGGACA CTCTTGGAAT GACAATGAAC 
CATTTCTTTT CCCTGATGCA GGCAGAACTG AACCGTTTCC AAGATACAAA GAGACTCCTT 
CAAGATTATT ACTGGGGAAT GGAAAGTAAA ATCCCAGTAG AGGACAACAA GAGATTTACT 
CAAATCCCTT TGGTCCAACT GGATAGTAAA GACAATTCCG AAAGCCAGCT TAGAATTCCT 
CTAGTTCCTC GAATATCCAT TTCTCTGGAA ACAGTTACAC CCAAACCAAA AACAAAATCA 
GTACTGAAGA GCAAGACGGA TAACTCCCTA GAAAACGTTG AGTCCAACTT TGAGGCCGAT 
GAAAAGTTGG TCATGGACAC CTGGCAGCAG GCTTCTTTAG CAGTATCTCA CATGGTGGCT 
GCTGAAATTC ATCAGAGGCT TATGGAAGAA GAAAAAGAAA ACCAGCCAGC AGACCCCAAA 
GAAAAATCTC CTCAGATGGG TGCAAATAAA AAAGTCAAAA AGGAGCCACC CAAGAAAAAA 
CAGGAAGACA AAAAACCCAA AGGTAAATCA TCACCTATGG CAGAAGCAAC TCCTGTCATA 
GTAACAACAG AGGAAATTGC TGAAATCAAA AGGAAAAATG AACTGAGGGT CAAAATAAAA 
GAAGAACACC TTGCTGCCTT GCAATTTGAA GAAATAGCCA CGCAATTTCG ACTTGAACTG 
ATAAAGACAA AAGCATTGGC TCTTCTTGAA GATTTAGTAA CAAAGGTGGT TGATGTATAT 
AAACTCATGG AAAAATGGCT TGGTGAGAGG TATTTGAATG AAATGGCCAG TATAGAAAAA 
TTAACTGACG TAGCTCGCTA TCACATTGAA ACATCTACAA AAATTCAGAA TGAACTTTAT 
TTAAGCCAAG AAGACTTCTT CATTAATGGC AATATAAAAG TCTTCCCAGA TCCTCCCCCA 
CCAATACGTC CTCCACCTGT AGAAAAGGAA GAAGATGGCA CCCTGACCAT TGAACAGCTT 
GACAGTCTTC GAGATCAGTT CTTAGATATG GCACCTAACG GCATAATAGG AAATAAAGCA 
TTTACTGACA TTCTGATCGA TTTGGTGACC CTGAACCTTG GCACAAACAA CTTTCCTAGT 
AGTTGGATGC ACCTTACCCA ACCTGAATTA CAGGAATTAA CATCTTTATT AACAGTCAAC 
TCCGAGTTCG TGGACTGGCG GAAGTTCCTG TTAGTAACCT CAATGCCTTG GCCCGTTCCC 
TCGGAGGAGG AGCTCCTTGA GACCCTTCAG AAGTTCAAGG CTGTGGATAA GGAGCAGTTG 
GGCACCATCA CTTTCGAGCA GTATATGCAG GCTGGTCTGT GGTTTACAGG AGATGAAGAT 
ATAAAAATTC CAGAAAATCC TCTTGAACCC CTTCCATTTA ATAGGCAGGA GCATCTTATA 
GAGTTTTTCT TTAGGCTATT TGCTGACTAT AAGAAGGATC CACCCCAGCT TGACTACACA 
CAGATGCTGC TTTACTTTGC TTGCCACCCA GACACCGTGG AAGGAGCCTA CAGGGCCCTC 
AGTGTGGCTG TTGGAACTCA TGTCTTCCAA CAAGTCAAAG CTTCCATTCC AAGTGCAGAA 
AAGACCTCCT CAACTGATGC AGGTCCAGCT GAGGAATTTC CTGAACCTGA GGGAAATGCT 
GCAAGAGAAG AAAGGAAATT AAAAGACGAC AGGGAGAAAA GGGAACAGAA GGATGAAGAA 
ATCCCTGAAA ATGCAAACAA TGAAAAGATG TCCATGGAAA CACTACTCAA AGTGTTCAAA 
GGGGGAAGTG AAGCACAGGA CTCCAACAGA TTTGCCAGCC ACCTAAAGAT AGAGAACATT 
TATGCAGAGG GCTTCATAAA AACATTTCAA GACCTAGGTG CCAAGAACCT GGAGCCAATT 
GAAGTCACTG TTCTCTTGAA GCATCCTTTT ATTCAAGACC TGATTTCAAA TTATTCAGAC 
TATAAGTTTC CTGTACACAT CATCTATGTA GTGGAAATGA TAGTGTGTGC AAAAGGTTGG 
GCACCACTTC CATCAGGAAA ACAGTGTGCA AGATGGAGAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953433.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808922.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

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CloneID	OPc85074
Clone ID Related Accession (Same CDS sequence)	XM_016953433.1
Accession Version	XM_016953433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATAAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCAC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808922.1
CDS	150..5546
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953433.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953433.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTCG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA TCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGT CCGGAAGGAA 
GAGGCAGAGC AGGAGCGGCT TGACATCATT AATAAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCAAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC CGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GAGCAAGACG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CATCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTATAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CACCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG CACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA CGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAGTTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCGTTC CCTCGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTCGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATAAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGCC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGGAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACAGGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAACA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCAC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953435.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808924.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

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CloneID	OPc85075
Clone ID Related Accession (Same CDS sequence)	XM_016953435.1
Accession Version	XM_016953435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCGAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTGA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGTTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAAAGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATA 
CTTAGTGGTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATGGTAA ATGCTATTAA CAAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGCC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG 
GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA 
TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT 
GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA 
CCAGTTCCTG AGGAAATGCC TTCGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC 
TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGATCAGCA TACTGTGCTT 
GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC AGATCACAAG 
CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT 
GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG 
GACATTTGTG ATGTCCGGAA GGAAGAGGCA GAGCAGGAGC GGCTTGACAT CATTAATAAG 
AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA 
GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA 
AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCAAA TCCCTTTGGT CCAACTGGAT 
AGTAAAGACA ATTCCGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT 
CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGAGCAA GACGGATAAC 
TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG 
CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG 
GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA 
AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT 
AAATCATCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA 
ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA 
TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT 
CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT 
GAGAGGTATT TGAATGAAAT GGCCAGTATA GAAAAATTAA CTGACGTAGC TCGCTATCAC 
ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT 
AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCACCAA TACGTCCTCC ACCTGTAGAA 
AAGGAAGAAG ATGGCACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA 
GATATGGCAC CTAACGGCAT AATAGGAAAT AAAGCATTTA CTGACATTCT GATCGATTTG 
GTGACCCTGA ACCTTGGCAC AAACAACTTT CCTAGTAGTT GGATGCACCT TACCCAACCT 
GAATTACAGG AATTAACATC TTTATTAACA GTCAACTCCG AGTTCGTGGA CTGGCGGAAG 
TTCCTGTTAG TAACCTCAAT GCCTTGGCCC GTTCCCTCGG AGGAGGAGCT CCTTGAGACC 
CTTCAGAAGT TCAAGGCTGT GGATAAGGAG CAGTTGGGCA CCATCACTTT CGAGCAGTAT 
ATGCAGGCTG GTCTGTGGTT TACAGGAGAT GAAGATATAA AAATTCCAGA AAATCCTCTT 
GAACCCCTTC CATTTAATAG GCAGGAGCAT CTTATAGAGT TTTTCTTTAG GCTATTTGCT 
GACTATAAGA AGGATCCACC CCAGCTTGAC TACACACAGA TGCTGCTTTA CTTTGCTTGC 
CACCCAGACA CCGTGGAAGG AGCCTACAGG GCCCTCAGTG TGGCTGTTGG AACTCATGTC 
TTCCAACAAG TCAAAGCTTC CATTCCAAGT GCAGAAAAGA CCTCCTCAAC TGATGCAGGT 
CCAGCTGAGG AATTTCCTGA ACCTGAGGGA AATGCTGCAA GAGAAGAAAG GAAATTAAAA 
GACGACAGGG AGAAAAGGGA ACAGAAGGAT GAAGAAATCC CTGAAAATGC AAACAATGAA 
AAGATGTCCA TGGAAACACT ACTCAAAGTG TTCAAAGGGG GAAGTGAAGC ACAGGACTCC 
AACAGATTTG CCAGCCACCT AAAGATAGAG AACATTTATG CAGAGGGCTT CATAAAAACA 
TTTCAAGACC TAGGTGCCAA GAACCTGGAG CCAATTGAAG TCACTGTTCT CTTGAAGCAT 
CCTTTTATTC AAGACCTGAT TTCAAATTAT TCAGACTATA AGTTTCCTGT ACACATCATC 
TATGTAGTGG AAATGATAGT GTGTGCAAAA GGTTGGGCAC CACTTCCATC AGGAAAACAG 
TGTGCAAGAT GGAGAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808924.1
CDS	128..5428
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQEQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953435.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953435.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCGAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTGA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGTTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAAAGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATA 
CTTAGTGGTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATGGTAA ATGCTATTAA CAAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGCC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG 
GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA 
TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT 
GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA 
CCAGTTCCTG AGGAAATGCC TTCGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC 
TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGATCAGCA TACTGTGCTT 
GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC AGATCACAAG 
CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT 
GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG 
GACATTTGTG ATGTCCGGAA GGAAGAGGCA GAGCAGGAGC GGCTTGACAT CATTAATAAG 
AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA 
GAACTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA 
AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCAAA TCCCTTTGGT CCAACTGGAT 
AGTAAAGACA ATTCCGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT 
CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGAGCAA GACGGATAAC 
TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG 
CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG 
GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA 
AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT 
AAATCATCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA 
ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA 
TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT 
CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT 
GAGAGGTATT TGAATGAAAT GGCCAGTATA GAAAAATTAA CTGACGTAGC TCGCTATCAC 
ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT 
AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCACCAA TACGTCCTCC ACCTGTAGAA 
AAGGAAGAAG ATGGCACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA 
GATATGGCAC CTAACGGCAT AATAGGAAAT AAAGCATTTA CTGACATTCT GATCGATTTG 
GTGACCCTGA ACCTTGGCAC AAACAACTTT CCTAGTAGTT GGATGCACCT TACCCAACCT 
GAATTACAGG AATTAACATC TTTATTAACA GTCAACTCCG AGTTCGTGGA CTGGCGGAAG 
TTCCTGTTAG TAACCTCAAT GCCTTGGCCC GTTCCCTCGG AGGAGGAGCT CCTTGAGACC 
CTTCAGAAGT TCAAGGCTGT GGATAAGGAG CAGTTGGGCA CCATCACTTT CGAGCAGTAT 
ATGCAGGCTG GTCTGTGGTT TACAGGAGAT GAAGATATAA AAATTCCAGA AAATCCTCTT 
GAACCCCTTC CATTTAATAG GCAGGAGCAT CTTATAGAGT TTTTCTTTAG GCTATTTGCT 
GACTATAAGA AGGATCCACC CCAGCTTGAC TACACACAGA TGCTGCTTTA CTTTGCTTGC 
CACCCAGACA CCGTGGAAGG AGCCTACAGG GCCCTCAGTG TGGCTGTTGG AACTCATGTC 
TTCCAACAAG TCAAAGCTTC CATTCCAAGT GCAGAAAAGA CCTCCTCAAC TGATGCAGGT 
CCAGCTGAGG AATTTCCTGA ACCTGAGGGA AATGCTGCAA GAGAAGAAAG GAAATTAAAA 
GACGACAGGG AGAAAAGGGA ACAGAAGGAT GAAGAAATCC CTGAAAATGC AAACAATGAA 
AAGATGTCCA TGGAAACACT ACTCAAAGTG TTCAAAGGGG GAAGTGAAGC ACAGGACTCC 
AACAGATTTG CCAGCCACCT AAAGATAGAG AACATTTATG CAGAGGGCTT CATAAAAACA 
TTTCAAGACC TAGGTGCCAA GAACCTGGAG CCAATTGAAG TCACTGTTCT CTTGAAGCAT 
CCTTTTATTC AAGACCTGAT TTCAAATTAT TCAGACTATA AGTTTCCTGT ACACATCATC 
TATGTAGTGG AAATGATAGT GTGTGCAAAA GGTTGGGCAC CACTTCCATC AGGAAAACAG 
TGTGCAAGAT GGAGAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953436.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808925.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

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CloneID	OPc85076
Clone ID Related Accession (Same CDS sequence)	XM_016953436.1
Accession Version	XM_016953436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAA TCCAAGCAGC TATTATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
AAAGCACTCG AGGCCCGAAA AATGATGAAA AAGAAAAAAG AGGCAGAAGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGAACA TGGTTGGAGA GTGGGCCTTA CCAGAAGAAA TGGTTGACAA TTTACCACCC 
TCCAACAATT GCATACTGGG CCATATTCTT CACAGGCTAG TTGAAAAATC TCTTCCTCCT 
CGAGCGGAAT CAACAACACC TGAATTACCT TCATTTGCTG TTAAAGGATG CTTATTGGGG 
AAAACATTAA GTGGAAAAAC TACCATTTTA AGGTCTCTAC AAAAAGACTT TCCTATACAG 
ATACTTTCTA TTGACACTCT TGTCCAAGAA GCTATCCAAG CATTTCATGA CAATGAAAAA 
GTCAGTGAGG TTCTACCAAT TCAGAAAAAA GATGAAGAAG ATGCTCTACC AGTTCTGCAA 
GAGGAGATTA AAGAAAGCCA GGATCCACAA CATGTATTTT CAGCTGGTCC AGTTTCAGAT 
GAAGTATTAC CAGAAACAGA AGGTGAAACA ATACTTAGTG GTAATGCTGA TAAAACACCA 
AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTTAAAAC TCACTACACG TGCTCAGCTT 
GGTGCAAAAT CAGAACAGTT GCTGAAGAAA GGAAAGAGCA TTCCTGATGT GCTGCTTGTT 
GACATCATGG TAAATGCTAT TAACAAGATA CCTGTGAATC AAGACTGTAT CCTAGATGGT 
TTTCCAATGA CTTTAAACCA AGCACAGCTT CTGGAAGAAG CTCTTACAGG CTGCAATAGA 
AACCTCACAG AAGTGGAAAG AAAAAAAGCA CAAAAATCCA CATTGGCTAT TGATCCTGCG 
ACTTCCAAAG AAATACCTCT TCCCTCTCCT GCATTTGATT TTGTCATATT ATTAGATGTT 
TCAGATACTT CCTCAATGAG TCGCATGAAT GATATTATAG CTGAAGAATT GTCCTATAAA 
ACTGCTCACG AAGATATCAG TCAACGTGTA GCTGCTGAAA ACCAAGATAA GGATGGAGAC 
CAAAATTTAA GAGACCAGAT ACAACATAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTA 
TTGGAGCAAT GGTTTTCAGA GCCAGAAAAT ATTTTGATAA AAATCAATGC TGAAATAGAT 
AAGGAATCTT TATGTGAAAA AGTAAAAGAA ATTCTTACGA CTGAAATAGC AAAAAAAAAG 
AATAAAGTTG AGAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGCAGC AGCTTCCCTG 
GCTGAGCTTC CACTTCCTAC ACCTCCTCCT GCTCCTCCTC CTGAACCAGA AAAAGAGAAG 
GAAATTCATC AAAGCCATGT GCCTTCAAAA ACTCCTACTG CAAAAGGAAA ACCTCAATCA 
GAAGCCCCGC ATGGTAAGCA AGAATCTCTT CAGGAAGGAA AAGGGAAGAA AGGTGAGACC 
GCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCATCAG GAGGAAAAGT ACCAGTAAAG 
AAATCACCTG CTGACTCTAC AGATACATCA CCTGTTGCAA TAGTGCCACA GCCACCTAAG 
CCAGGATCAG AAGAATGGGT CTATGTGAAT GAACCAGTTC CTGAGGAAAT GCCTTCGTTT 
TTAGTACCTT ACTGGGAACT AATAGAAAAT TCCTATATAA ACACCATCAA AACAGTACTC 
AGGCATCTGA GGGAAGATCA GCATACTGTG CTTGCTTACT TATATGAGAT TAGAACAAGT 
TTCCAGGAGT TTCTAAAGCG TCCAGATCAC AAGCAAGATT TTGTAGCTCA ATGGCAGGCT 
GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAACTACAT 
CAACGAGTGA ATGATCTGCG AGACCGCCTG TGGGACATTT GTGATGTCCG GAAGGAAGAG 
GCAGAGCAGG AGCGGCTTGA CATCATTAAT AAGAGCTGGT TACAGGACAC TCTTGGAATG 
ACAATGAACC ATTTCTTTTC CCTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACAAAG 
AGACTCCTTC AAGATTATTA CTGGGGAATG GAAAGTAAAA TCCCAGTAGA GGACAACAAG 
AGATTTACTC AAATCCCTTT GGTCCAACTG GATAGTAAAG ACAATTCCGA AAGCCAGCTT 
AGAATTCCTC TAGTTCCTCG AATATCCATT TCTCTGGAAA CAGTTACACC CAAACCAAAA 
ACAAAATCAG TACTGAAGAG CAAGACGGAT AACTCCCTAG AAAACGTTGA GTCCAACTTT 
GAGGCCGATG AAAAGTTGGT CATGGACACC TGGCAGCAGG CTTCTTTAGC AGTATCTCAC 
ATGGTGGCTG CTGAAATTCA TCAGAGGCTT ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA 
GACCCCAAAG AAAAATCTCC TCAGATGGGT GCAAATAAAA AAGTCAAAAA GGAGCCACCC 
AAGAAAAAAC AGGAAGACAA AAAACCCAAA GGTAAATCAT CACCTATGGC AGAAGCAACT 
CCTGTCATAG TAACAACAGA GGAAATTGCT GAAATCAAAA GGAAAAATGA ACTGAGGGTC 
AAAATAAAAG AAGAACACCT TGCTGCCTTG CAATTTGAAG AAATAGCCAC GCAATTTCGA 
CTTGAACTGA TAAAGACAAA AGCATTGGCT CTTCTTGAAG ATTTAGTAAC AAAGGTGGTT 
GATGTATATA AACTCATGGA AAAATGGCTT GGTGAGAGGT ATTTGAATGA AATGGCCAGT 
ATAGAAAAAT TAACTGACGT AGCTCGCTAT CACATTGAAA CATCTACAAA AATTCAGAAT 
GAACTTTATT TAAGCCAAGA AGACTTCTTC ATTAATGGCA ATATAAAAGT CTTCCCAGAT 
CCTCCCCCAC CAATACGTCC TCCACCTGTA GAAAAGGAAG AAGATGGCAC CCTGACCATT 
GAACAGCTTG ACAGTCTTCG AGATCAGTTC TTAGATATGG CACCTAACGG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA GTTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCGTTCCCT CGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTCGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATA AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGCCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GGAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA GGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAACAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCACTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808925.1
CDS	468..5321
Translation	MAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953436.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953436.1

ORF Insert Sequence:

ATGGCCAAAA TCCAAGCAGC TATTATACAG ATTCCTAAAC CTGCATCAAA TCGTACTTTG 
AAAGCACTCG AGGCCCGAAA AATGATGAAA AAGAAAAAAG AGGCAGAAGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGAACA TGGTTGGAGA GTGGGCCTTA CCAGAAGAAA TGGTTGACAA TTTACCACCC 
TCCAACAATT GCATACTGGG CCATATTCTT CACAGGCTAG TTGAAAAATC TCTTCCTCCT 
CGAGCGGAAT CAACAACACC TGAATTACCT TCATTTGCTG TTAAAGGATG CTTATTGGGG 
AAAACATTAA GTGGAAAAAC TACCATTTTA AGGTCTCTAC AAAAAGACTT TCCTATACAG 
ATACTTTCTA TTGACACTCT TGTCCAAGAA GCTATCCAAG CATTTCATGA CAATGAAAAA 
GTCAGTGAGG TTCTACCAAT TCAGAAAAAA GATGAAGAAG ATGCTCTACC AGTTCTGCAA 
GAGGAGATTA AAGAAAGCCA GGATCCACAA CATGTATTTT CAGCTGGTCC AGTTTCAGAT 
GAAGTATTAC CAGAAACAGA AGGTGAAACA ATACTTAGTG GTAATGCTGA TAAAACACCA 
AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTTAAAAC TCACTACACG TGCTCAGCTT 
GGTGCAAAAT CAGAACAGTT GCTGAAGAAA GGAAAGAGCA TTCCTGATGT GCTGCTTGTT 
GACATCATGG TAAATGCTAT TAACAAGATA CCTGTGAATC AAGACTGTAT CCTAGATGGT 
TTTCCAATGA CTTTAAACCA AGCACAGCTT CTGGAAGAAG CTCTTACAGG CTGCAATAGA 
AACCTCACAG AAGTGGAAAG AAAAAAAGCA CAAAAATCCA CATTGGCTAT TGATCCTGCG 
ACTTCCAAAG AAATACCTCT TCCCTCTCCT GCATTTGATT TTGTCATATT ATTAGATGTT 
TCAGATACTT CCTCAATGAG TCGCATGAAT GATATTATAG CTGAAGAATT GTCCTATAAA 
ACTGCTCACG AAGATATCAG TCAACGTGTA GCTGCTGAAA ACCAAGATAA GGATGGAGAC 
CAAAATTTAA GAGACCAGAT ACAACATAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTA 
TTGGAGCAAT GGTTTTCAGA GCCAGAAAAT ATTTTGATAA AAATCAATGC TGAAATAGAT 
AAGGAATCTT TATGTGAAAA AGTAAAAGAA ATTCTTACGA CTGAAATAGC AAAAAAAAAG 
AATAAAGTTG AGAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGCAGC AGCTTCCCTG 
GCTGAGCTTC CACTTCCTAC ACCTCCTCCT GCTCCTCCTC CTGAACCAGA AAAAGAGAAG 
GAAATTCATC AAAGCCATGT GCCTTCAAAA ACTCCTACTG CAAAAGGAAA ACCTCAATCA 
GAAGCCCCGC ATGGTAAGCA AGAATCTCTT CAGGAAGGAA AAGGGAAGAA AGGTGAGACC 
GCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCATCAG GAGGAAAAGT ACCAGTAAAG 
AAATCACCTG CTGACTCTAC AGATACATCA CCTGTTGCAA TAGTGCCACA GCCACCTAAG 
CCAGGATCAG AAGAATGGGT CTATGTGAAT GAACCAGTTC CTGAGGAAAT GCCTTCGTTT 
TTAGTACCTT ACTGGGAACT AATAGAAAAT TCCTATATAA ACACCATCAA AACAGTACTC 
AGGCATCTGA GGGAAGATCA GCATACTGTG CTTGCTTACT TATATGAGAT TAGAACAAGT 
TTCCAGGAGT TTCTAAAGCG TCCAGATCAC AAGCAAGATT TTGTAGCTCA ATGGCAGGCT 
GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAACTACAT 
CAACGAGTGA ATGATCTGCG AGACCGCCTG TGGGACATTT GTGATGTCCG GAAGGAAGAG 
GCAGAGCAGG AGCGGCTTGA CATCATTAAT AAGAGCTGGT TACAGGACAC TCTTGGAATG 
ACAATGAACC ATTTCTTTTC CCTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACAAAG 
AGACTCCTTC AAGATTATTA CTGGGGAATG GAAAGTAAAA TCCCAGTAGA GGACAACAAG 
AGATTTACTC AAATCCCTTT GGTCCAACTG GATAGTAAAG ACAATTCCGA AAGCCAGCTT 
AGAATTCCTC TAGTTCCTCG AATATCCATT TCTCTGGAAA CAGTTACACC CAAACCAAAA 
ACAAAATCAG TACTGAAGAG CAAGACGGAT AACTCCCTAG AAAACGTTGA GTCCAACTTT 
GAGGCCGATG AAAAGTTGGT CATGGACACC TGGCAGCAGG CTTCTTTAGC AGTATCTCAC 
ATGGTGGCTG CTGAAATTCA TCAGAGGCTT ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA 
GACCCCAAAG AAAAATCTCC TCAGATGGGT GCAAATAAAA AAGTCAAAAA GGAGCCACCC 
AAGAAAAAAC AGGAAGACAA AAAACCCAAA GGTAAATCAT CACCTATGGC AGAAGCAACT 
CCTGTCATAG TAACAACAGA GGAAATTGCT GAAATCAAAA GGAAAAATGA ACTGAGGGTC 
AAAATAAAAG AAGAACACCT TGCTGCCTTG CAATTTGAAG AAATAGCCAC GCAATTTCGA 
CTTGAACTGA TAAAGACAAA AGCATTGGCT CTTCTTGAAG ATTTAGTAAC AAAGGTGGTT 
GATGTATATA AACTCATGGA AAAATGGCTT GGTGAGAGGT ATTTGAATGA AATGGCCAGT 
ATAGAAAAAT TAACTGACGT AGCTCGCTAT CACATTGAAA CATCTACAAA AATTCAGAAT 
GAACTTTATT TAAGCCAAGA AGACTTCTTC ATTAATGGCA ATATAAAAGT CTTCCCAGAT 
CCTCCCCCAC CAATACGTCC TCCACCTGTA GAAAAGGAAG AAGATGGCAC CCTGACCATT 
GAACAGCTTG ACAGTCTTCG AGATCAGTTC TTAGATATGG CACCTAACGG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA GTTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCGTTCCCT CGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTCGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATA AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGCCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GGAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA GGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAACAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCACTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953437.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808926.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OPc85077
Clone ID Related Accession (Same CDS sequence)	XM_016953437.1
Accession Version	XM_016953437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG GCTGGAGTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808926.1
CDS	150..4994
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEAGVQ

Target ORF information:

RefSeq Version	XM_016953437.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953437.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAGT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CGTTCCCTCG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TCGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG GCTGGAGTGC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953438.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808927.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OPc85078
Clone ID Related Accession (Same CDS sequence)	XM_016953438.1
Accession Version	XM_016953438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTTA TGGTTCTGAA GGTTGGAAGA TTCAAGACTG GGTATCTGGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGAACA TGGTTGGAGA GTGGGCCTTA CCAGAAGAAA TGGTTGACAA TTTACCACCC 
TCCAACAATT GCATACTGGG CCATATTCTT CACAGGCTAG TTGAAAAATC TCTTCCTCCT 
CGAGCGGAAT CAACAACACC TGAATTACCT TCATTTGCTG TTAAAGGATG CTTATTGGGG 
AAAACATTAA GTGGAAAAAC TACCATTTTA AGGTCTCTAC AAAAAGACTT TCCTATACAG 
ATACTTTCTA TTGACACTCT TGTCCAAGAA GCTATCCAAG CATTTCATGA CAATGAAAAA 
GTCAGTGAGG TTCTACCAAT TCAGAAAAAA GATGAAGAAG ATGCTCTACC AGTTCTGCAA 
GAGGAGATTA AAGAAAGCCA GGATCCACAA CATGTATTTT CAGCTGGTCC AGTTTCAGAT 
GAAGTATTAC CAGAAACAGA AGGTGAAACA ATACTTAGTG GTAATGCTGA TAAAACACCA 
AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTTAAAAC TCACTACACG TGCTCAGCTT 
GGTGCAAAAT CAGAACAGTT GCTGAAGAAA GGAAAGAGCA TTCCTGATGT GCTGCTTGTT 
GACATCATGG TAAATGCTAT TAACAAGATA CCTGTGAATC AAGACTGTAT CCTAGATGGT 
TTTCCAATGA CTTTAAACCA AGCACAGCTT CTGGAAGAAG CTCTTACAGG CTGCAATAGA 
AACCTCACAG AAGTGGAAAG AAAAAAAGCA CAAAAATCCA CATTGGCTAT TGATCCTGCG 
ACTTCCAAAG AAATACCTCT TCCCTCTCCT GCATTTGATT TTGTCATATT ATTAGATGTT 
TCAGATACTT CCTCAATGAG TCGCATGAAT GATATTATAG CTGAAGAATT GTCCTATAAA 
ACTGCTCACG AAGATATCAG TCAACGTGTA GCTGCTGAAA ACCAAGATAA GGATGGAGAC 
CAAAATTTAA GAGACCAGAT ACAACATAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTA 
TTGGAGCAAT GGTTTTCAGA GCCAGAAAAT ATTTTGATAA AAATCAATGC TGAAATAGAT 
AAGGAATCTT TATGTGAAAA AGTAAAAGAA ATTCTTACGA CTGAAATAGC AAAAAAAAAG 
AATAAAGTTG AGAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGCAGC AGCTTCCCTG 
GCTGAGCTTC CACTTCCTAC ACCTCCTCCT GCTCCTCCTC CTGAACCAGA AAAAGAGAAG 
GAAATTCATC AAAGCCATGT GCCTTCAAAA ACTCCTACTG CAAAAGGAAA ACCTCAATCA 
GAAGCCCCGC ATGGTAAGCA AGAATCTCTT CAGGAAGGAA AAGGGAAGAA AGGTGAGACC 
GCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCATCAG GAGGAAAAGT ACCAGTAAAG 
AAATCACCTG CTGACTCTAC AGATACATCA CCTGTTGCAA TAGTGCCACA GCCACCTAAG 
CCAGGATCAG AAGAATGGGT CTATGTGAAT GAACCAGTTC CTGAGGAAAT GCCTTCGTTT 
TTAGTACCTT ACTGGGAACT AATAGAAAAT TCCTATATAA ACACCATCAA AACAGTACTC 
AGGCATCTGA GGGAAGATCA GCATACTGTG CTTGCTTACT TATATGAGAT TAGAACAAGT 
TTCCAGGAGT TTCTAAAGCG TCCAGATCAC AAGCAAGATT TTGTAGCTCA ATGGCAGGCT 
GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAACTACAT 
CAACGAGTGA ATGATCTGCG AGACCGCCTG TGGGACATTT GTGATGTCCG GAAGGAAGAG 
GCAGAGCAGG AGCGGCTTGA CATCATTAAT AAGAGCTGGT TACAGGACAC TCTTGGAATG 
ACAATGAACC ATTTCTTTTC CCTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACAAAG 
AGACTCCTTC AAGATTATTA CTGGGGAATG GAAAGTAAAA TCCCAGTAGA GGACAACAAG 
AGATTTACTC AAATCCCTTT GGTCCAACTG GATAGTAAAG ACAATTCCGA AAGCCAGCTT 
AGAATTCCTC TAGTTCCTCG AATATCCATT TCTCTGGAAA CAGTTACACC CAAACCAAAA 
ACAAAATCAG TACTGAAGAG CAAGACGGAT AACTCCCTAG AAAACGTTGA GTCCAACTTT 
GAGGCCGATG AAAAGTTGGT CATGGACACC TGGCAGCAGG CTTCTTTAGC AGTATCTCAC 
ATGGTGGCTG CTGAAATTCA TCAGAGGCTT ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA 
GACCCCAAAG AAAAATCTCC TCAGATGGGT GCAAATAAAA AAGTCAAAAA GGAGCCACCC 
AAGAAAAAAC AGGAAGACAA AAAACCCAAA GGTAAATCAT CACCTATGGC AGAAGCAACT 
CCTGTCATAG TAACAACAGA GGAAATTGCT GAAATCAAAA GGAAAAATGA ACTGAGGGTC 
AAAATAAAAG AAGAACACCT TGCTGCCTTG CAATTTGAAG AAATAGCCAC GCAATTTCGA 
CTTGAACTGA TAAAGACAAA AGCATTGGCT CTTCTTGAAG ATTTAGTAAC AAAGGTGGTT 
GATGTATATA AACTCATGGA AAAATGGCTT GGTGAGAGGT ATTTGAATGA AATGGCCAGT 
ATAGAAAAAT TAACTGACGT AGCTCGCTAT CACATTGAAA CATCTACAAA AATTCAGAAT 
GAACTTTATT TAAGCCAAGA AGACTTCTTC ATTAATGGCA ATATAAAAGT CTTCCCAGAT 
CCTCCCCCAC CAATACGTCC TCCACCTGTA GAAAAGGAAG AAGATGGCAC CCTGACCATT 
GAACAGCTTG ACAGTCTTCG AGATCAGTTC TTAGATATGG CACCTAACGG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA GTTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCGTTCCCT CGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTCGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATA AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGCCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GGAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA GGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAACAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCACTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808927.1
CDS	357..5150
Translation	MSLMVLKVGRFKTGYLDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNGIIGNKAFTDILIDLVTLNLGTNNFPSSWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPVPSEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYKKDPPQLDYTQMLLYFACHPDTVEGAYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEGNAAREERKLKDDREKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVTVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_016953438.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953438.1

ORF Insert Sequence:

ATGTCACTTA TGGTTCTGAA GGTTGGAAGA TTCAAGACTG GGTATCTGGA TGTGGCTGAT 
GAAATTAAGA AGTTCGAAGC ATTAATAAAA AAGGATCTCC AAGCAAAAGA AAGTGCATCC 
AAGACTTCTT TAGATACAGC AGGCCAGACA ACCACCGATT TGTTAAATAC TTACTCAGAT 
GACGAGTACA TTAAAAAAAT CCAGAAGCGC CTTGAAGAAG ATGCTTTTGC ACGAGAGCAA 
AGGGAGAAAA GACGGCGGAA ATTGTTAATG GACCAGTTAA TAGCCCACGA AGCACAAGAG 
GAGGCTTATC GGGAGGAACA GCTGATTAAC CGGCTGATGC GGCAGTCCCA GCAGGAGCGC 
AGGATTGCCG TGCAGCTCAT GCATGTTCGG CATGAAAAGG AAGTTTTATG GCAAAACAGA 
ATTTTCAGAG AAAAACAACA TGAGGAAAGA CGACTTAAAG ATTTCCAGGA TGCTCTTGAT 
CGAGAAGCGG CTTTGGCAAA ACAAGCCAAG ATTGACTTTG AAGAACAATT CCTTAAAGAA 
AAGAGATTTC ATGATCAGAT TGCTGTGGAA AGAGCTCAAG CTCGTTATGA AAAGCATTAT 
TCAGTATGTG CAGAAATTTT GGATCAAATA GTTGATTTGT CCACTAAAGT GGCAGACTAT 
CGAATGTTGA CAAATAATCT GATTCCGTAT AAGTTGATGC ATGATTGGAA GGAACTATTT 
TTTAATGCAA AACCCATATA TGAACAAGCC TCTGTTAAGA CACTACCTGC TGACCCCTCA 
AGAGAACAAC TTACAGAACT GGAGAAAAGG GACTTGCTAG ATACCAATGA TTATGAAGAA 
TATAAGAACA TGGTTGGAGA GTGGGCCTTA CCAGAAGAAA TGGTTGACAA TTTACCACCC 
TCCAACAATT GCATACTGGG CCATATTCTT CACAGGCTAG TTGAAAAATC TCTTCCTCCT 
CGAGCGGAAT CAACAACACC TGAATTACCT TCATTTGCTG TTAAAGGATG CTTATTGGGG 
AAAACATTAA GTGGAAAAAC TACCATTTTA AGGTCTCTAC AAAAAGACTT TCCTATACAG 
ATACTTTCTA TTGACACTCT TGTCCAAGAA GCTATCCAAG CATTTCATGA CAATGAAAAA 
GTCAGTGAGG TTCTACCAAT TCAGAAAAAA GATGAAGAAG ATGCTCTACC AGTTCTGCAA 
GAGGAGATTA AAGAAAGCCA GGATCCACAA CATGTATTTT CAGCTGGTCC AGTTTCAGAT 
GAAGTATTAC CAGAAACAGA AGGTGAAACA ATACTTAGTG GTAATGCTGA TAAAACACCA 
AAAGCTGAAG AAGTCAAATC AAGTGATAGT TTCTTAAAAC TCACTACACG TGCTCAGCTT 
GGTGCAAAAT CAGAACAGTT GCTGAAGAAA GGAAAGAGCA TTCCTGATGT GCTGCTTGTT 
GACATCATGG TAAATGCTAT TAACAAGATA CCTGTGAATC AAGACTGTAT CCTAGATGGT 
TTTCCAATGA CTTTAAACCA AGCACAGCTT CTGGAAGAAG CTCTTACAGG CTGCAATAGA 
AACCTCACAG AAGTGGAAAG AAAAAAAGCA CAAAAATCCA CATTGGCTAT TGATCCTGCG 
ACTTCCAAAG AAATACCTCT TCCCTCTCCT GCATTTGATT TTGTCATATT ATTAGATGTT 
TCAGATACTT CCTCAATGAG TCGCATGAAT GATATTATAG CTGAAGAATT GTCCTATAAA 
ACTGCTCACG AAGATATCAG TCAACGTGTA GCTGCTGAAA ACCAAGATAA GGATGGAGAC 
CAAAATTTAA GAGACCAGAT ACAACATAGA ATTATAGGCT TCTTGGACAA CTGGCCTTTA 
TTGGAGCAAT GGTTTTCAGA GCCAGAAAAT ATTTTGATAA AAATCAATGC TGAAATAGAT 
AAGGAATCTT TATGTGAAAA AGTAAAAGAA ATTCTTACGA CTGAAATAGC AAAAAAAAAG 
AATAAAGTTG AGAAGAAATT AGAAGAAAAG GAAGCTGAGA AAAAAGCAGC AGCTTCCCTG 
GCTGAGCTTC CACTTCCTAC ACCTCCTCCT GCTCCTCCTC CTGAACCAGA AAAAGAGAAG 
GAAATTCATC AAAGCCATGT GCCTTCAAAA ACTCCTACTG CAAAAGGAAA ACCTCAATCA 
GAAGCCCCGC ATGGTAAGCA AGAATCTCTT CAGGAAGGAA AAGGGAAGAA AGGTGAGACC 
GCACTCAAAA GAAAAGGTTC TCCTAAAGGA AAATCATCAG GAGGAAAAGT ACCAGTAAAG 
AAATCACCTG CTGACTCTAC AGATACATCA CCTGTTGCAA TAGTGCCACA GCCACCTAAG 
CCAGGATCAG AAGAATGGGT CTATGTGAAT GAACCAGTTC CTGAGGAAAT GCCTTCGTTT 
TTAGTACCTT ACTGGGAACT AATAGAAAAT TCCTATATAA ACACCATCAA AACAGTACTC 
AGGCATCTGA GGGAAGATCA GCATACTGTG CTTGCTTACT TATATGAGAT TAGAACAAGT 
TTCCAGGAGT TTCTAAAGCG TCCAGATCAC AAGCAAGATT TTGTAGCTCA ATGGCAGGCT 
GATTTCAACT CCCTTCCTGA TGACCTGTGG GATGATGAGG AAACAAAGGC TGAACTACAT 
CAACGAGTGA ATGATCTGCG AGACCGCCTG TGGGACATTT GTGATGTCCG GAAGGAAGAG 
GCAGAGCAGG AGCGGCTTGA CATCATTAAT AAGAGCTGGT TACAGGACAC TCTTGGAATG 
ACAATGAACC ATTTCTTTTC CCTGATGCAG GCAGAACTGA ACCGTTTCCA AGATACAAAG 
AGACTCCTTC AAGATTATTA CTGGGGAATG GAAAGTAAAA TCCCAGTAGA GGACAACAAG 
AGATTTACTC AAATCCCTTT GGTCCAACTG GATAGTAAAG ACAATTCCGA AAGCCAGCTT 
AGAATTCCTC TAGTTCCTCG AATATCCATT TCTCTGGAAA CAGTTACACC CAAACCAAAA 
ACAAAATCAG TACTGAAGAG CAAGACGGAT AACTCCCTAG AAAACGTTGA GTCCAACTTT 
GAGGCCGATG AAAAGTTGGT CATGGACACC TGGCAGCAGG CTTCTTTAGC AGTATCTCAC 
ATGGTGGCTG CTGAAATTCA TCAGAGGCTT ATGGAAGAAG AAAAAGAAAA CCAGCCAGCA 
GACCCCAAAG AAAAATCTCC TCAGATGGGT GCAAATAAAA AAGTCAAAAA GGAGCCACCC 
AAGAAAAAAC AGGAAGACAA AAAACCCAAA GGTAAATCAT CACCTATGGC AGAAGCAACT 
CCTGTCATAG TAACAACAGA GGAAATTGCT GAAATCAAAA GGAAAAATGA ACTGAGGGTC 
AAAATAAAAG AAGAACACCT TGCTGCCTTG CAATTTGAAG AAATAGCCAC GCAATTTCGA 
CTTGAACTGA TAAAGACAAA AGCATTGGCT CTTCTTGAAG ATTTAGTAAC AAAGGTGGTT 
GATGTATATA AACTCATGGA AAAATGGCTT GGTGAGAGGT ATTTGAATGA AATGGCCAGT 
ATAGAAAAAT TAACTGACGT AGCTCGCTAT CACATTGAAA CATCTACAAA AATTCAGAAT 
GAACTTTATT TAAGCCAAGA AGACTTCTTC ATTAATGGCA ATATAAAAGT CTTCCCAGAT 
CCTCCCCCAC CAATACGTCC TCCACCTGTA GAAAAGGAAG AAGATGGCAC CCTGACCATT 
GAACAGCTTG ACAGTCTTCG AGATCAGTTC TTAGATATGG CACCTAACGG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA GTTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCGTTCCCT CGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTCGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATA AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGCCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GGAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA GGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAACAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCACTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_016953439.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016808928.1 Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

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CloneID	OPc85079
Clone ID Related Accession (Same CDS sequence)	XM_016953439.1
Accession Version	XM_016953439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	sperm flagellar protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006472.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGAGT GCTCTGAGAA CTGCTTTCAA AGGCCTTCAC CTTCGACCTG TGATGCCTTC 
TCAAATAGGA GACCGGAAGT GGCTTCATGG ATTACAGGCA GCCCTCCAGA ACTTTCTCAA 
GGGATATCCT GCAATTGTTC GGCAACTGCA CTCAGCAGGC AGAGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016808928.1
CDS	150..4763
Translation	MSEILCHWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLHLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNLKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEARKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPADPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLVEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKKDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETILSGNADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIMVNAINKIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVPSKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPSFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDVRKEEAEQERLDIINKSWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTQIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKSKTDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSSPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASIEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPPIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPNECSENCFQRPSPSTCDAFSNRRPEVASWITGSPPELSQGISCNCSATALSRQRQK

Target ORF information:

RefSeq Version	XM_016953439.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016953439.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA CTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC TTGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATCG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCGAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTG ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGTT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAAAGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT ACTTAGTGGT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATGGTA AATGCTATTA ACAAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGC CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTCGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGATCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CAGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGTCCGGA AGGAAGAGGC AGAGCAGGAG CGGCTTGACA TCATTAATAA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAACTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCAA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCCGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGAGCA AGACGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCATCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAT AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCACCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGCACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAACGAGT GCTCTGAGAA CTGCTTTCAA AGGCCTTCAC CTTCGACCTG TGATGCCTTC 
TCAAATAGGA GACCGGAAGT GGCTTCATGG ATTACAGGCA GCCCTCCAGA ACTTTCTCAA 
GGGATATCCT GCAATTGTTC GGCAACTGCA CTCAGCAGGC AGAGGCAAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPEF2
Entrez Gene ID	741133
Full Name	sperm flagellar 2
General protein information	Preferred Names sperm flagellar 2 Names sperm flagellar protein 2 KPL2 protein
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

Canis lupus familiaris (dog)	SPEF2	XP_546353.2
Gallus gallus (chicken)	SPEF2	XP_003643024.2
Danio rerio (zebrafish)	spef2	NP_001121704.1
Homo sapiens (human)	SPEF2	NP_079143.3
	LOC703498	XP_001091802.1
Mus musculus (house mouse)	Spef2	NP_796097.2
Pan troglodytes (chimpanzee)	SPEF2	XP_001143539.2
Bos taurus (cattle)	SPEF2	XP_002696427.2
Rattus norvegicus (Norway rat)	Spef2	NP_072142.1
Xenopus tropicalis (tropical clawed frog)	spef2	XP_002940003.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPEF2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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