SPEF2 cDNA ORF clone, Bos taurus(cattle)

The following SPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa94132 XM_015468369.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94132 XM_015459107.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa94133 XM_015468370.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94133 XM_015468371.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94133 XM_015459109.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94133 XM_015468372.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94133 XM_015459108.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa94133 XM_015459110.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa30690 XM_015468367.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa30690 XM_002696381.5
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa60102 XM_015468368.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa60102 XM_010816825.3
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa228153 XM_024981501.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa228154 XM_024981503.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa228154 XM_024981502.1
Latest version!
Bos taurus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
View Old Accession Versions >>
OBa30690 XM_002696381.4 Bos taurus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa60102 XM_010816825.2 Bos taurus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa94132
    Clone ID Related Accession (Same CDS sequence) XM_015468369.1 , XM_015459107.1
    Accession Version XM_015468369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGGTTCTC CTAGAGGAAA ATCGCTAGGA
    GGGAAGATTC CACTCAAGAA ATCACCTGCC GAGTCTACAG ATGTTTCACC GGTCCCAGTA
    GTGCCAGCCC CATCGAAGCC CGGATCAGAA GAGTGGGTGT ATGTAAATGA ACCAATTCCT
    GAGGAAATAC CATCATTTTT AGTACCATAC TGGAAACTGA TAGAAAATTC CTATATAAAT
    TCCATCAAAA CAGTACTCAG GCAGTTGAGA GAAGACCAGC ATGCCGTACT TGCTTACTTA
    TATGATATTA GAACAGGCTT CCAGCAGTTT CTGAGGCGTC CAGACCACAA GCAAGACTTC
    GTGTCTCAGT GGCAGGCGGA TTTCAACTCC CTTCCTGACG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AGCTGCACCA AAGAGTGAAT GATTTCCGGG ACCGCCTGTG GGACATTTGC
    GATGCCCGCA AGGAAGAAGC AGAGCAGGAG CGTCTGGACA TCATTAACGA GAGCTGGTTG
    CAGGACTCGA CGGGAATTGC CATGAACCAT TTCTTCTCAC TGATGCAGGC AGAACTGAAC
    CGTTTCCAAG ATACGAAGAG ACTCCTGCAA GATTATTACA GGGCCATGGA GTGTAAAATT
    CCAATGGATG ACACTAAGAG ATTCACTCGA GTCCCACTGG TCCAACTGGA TAGTAAAGAT
    ATTTTGGAAA GCCAGCTTAG AATTCCCCTG GTTCCTCGAA GATCCATTTC TCCAGAGTCA
    GCTTTATCCA AACCAAAAAC AAAAAGCATC CTGAAGGGCA AGATCGATTA CATGCTGGAA
    AGTGTAGAGT TAAACTTTGA GGCTGATGAG AAGATGGTGA TGGATACGTG GCAGCAGTCT
    TCTTTGGCGA TATCTCACAT GGTGGCTGCT GAAATTCACC AGAGGCTCAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA AACAAAAGAA AAACTTCCTC AGGCGGCTGC AAATAAAAAA
    GTGAAAAAGG AGCCTCCGCC ACCACCACCA CCACCCAAGA AAAAAAAAGA GGACAAAAAA
    GGAAAAGGTA AATCCCCACC TATGACAGAA GCTGCTCCTG TAGTATTGCC AGCAGAAGAG
    ACTGAGGAAG TGGAAGTGAA AAACGAACTG AAGCTCAGAA TAAAGGAAGA GCATCTTGCT
    GCCCTGCAAT TTGAAGAGAT AGCTACACAG TTCCGACTTG AACTGATAAA GGTAAAAGCA
    TTGGCTTTCC TGGAAGAGTT AGTAATAAAG GCTGTTGATG TATATAGACT CATGGAAAAG
    TGGCTCGGTG AGAGGTACTT GAATGAAATG GCCAGTGTGG AAAAACTAAC TGGGGTAGCT
    CGCTATCACA TTGAAACATC AACCAAAATT CAGAATGAAC TTTATTTAGA CCAAGAAGAC
    TTCTTCATTA ATGGCGATAT CAAAGTCTTT CCAGATCCAC CTCCTCCGGT ACGCCCTCCA
    CCTGTGGAAA AGGAGGAAAA CGGCACCCTG ACCATCGAGC AGCTGGACAA CCTCCGGGAT
    CAGTTTTTAG ATATAGCGCC CAAAGGTATA ATAGGAAATA AAGCATTTGC TGACCTTCTG
    CTGGATTTGG TAACCCTGAA CCTTGGAACA AACAACTTTC CTAGTAGTTG GATGAACCTC
    ACTCAACCTG AATTGCAGGA GTTAGCATCT TTGTTGGTAA CAAACTCGGA GTCCGTGGAC
    TGGCGGAAAT TCTTGTTAGT AGTAGCGCTG CCTTGGCCCA TCCCCCTGGA GGAGGAGCTT
    CTGGAGACCC TGCAGAGGTT CAAGGCCCTG GATGCGGAGC AGCTGGGCAC CATCACTTAC
    GAACAGTATA AGCAGGCTGG CCTGTGGTTT ACAGGAGACG AAGATATCAA AATTCCAGAA
    AATCCTCTTG AACCCCTGCC ATTCAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    TTGTTTGCTG ACTGTGAGAA GGATCCACCC CAACTTGACT ACACGCAGAT GCTGCTTTAT
    TTTGCTTGCC ATCCAGACAG TACAGAAGGG GTCTACAGGG CCCTCAGCGT AGCCATTGGG
    ACTCACGTCT TCCAGCCAAT TGAAATGCCT CATCCTGTTG CAGAAAAGAC CTCTTCCTTT
    ACTGATATGA GTCCCATTGA GGAATATCCT GAACTTGAGG ACAACTTTAT GAGTGAGGAA
    AGGGAATTAC AAGAGGATGG GGAGGAAAAG GAAGAAGAAA TCCCTGAAAA TGCAAATACG
    GAAATGATTT CCATGCAAAC ATTACTCCAA GTGTTCCGAG GAGGAAATGA AGTACTGGAC
    GCTAACAGAT TTGCCAGCCA CCAAAAGTTA GAGAACACTT ATTTAGAGAA CTTCATAAAA
    GTGTTTCAGG ACCTAGGTTC CAAGAACTTA GAGCCAATTG AAGTTGCTGT TCTTCTGAAG
    CATCCTTTTA TTCAAGACCT GATTTCAAGT TATCCAGATT ATAAGATCCC TGATGTTAAG
    ATAATTCTCC AAAGGAGTGA GCATGTACAA GGAAGTGATG AAGAGAGGTC TCCTTCAAGA
    CTTACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323855.1
    CDS114..5354
    Translation

    Target ORF information:

    RefSeq Version XM_015468369.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGGTTCTC CTAGAGGAAA ATCGCTAGGA
    GGGAAGATTC CACTCAAGAA ATCACCTGCC GAGTCTACAG ATGTTTCACC GGTCCCAGTA
    GTGCCAGCCC CATCGAAGCC CGGATCAGAA GAGTGGGTGT ATGTAAATGA ACCAATTCCT
    GAGGAAATAC CATCATTTTT AGTACCATAC TGGAAACTGA TAGAAAATTC CTATATAAAT
    TCCATCAAAA CAGTACTCAG GCAGTTGAGA GAAGACCAGC ATGCCGTACT TGCTTACTTA
    TATGATATTA GAACAGGCTT CCAGCAGTTT CTGAGGCGTC CAGACCACAA GCAAGACTTC
    GTGTCTCAGT GGCAGGCGGA TTTCAACTCC CTTCCTGACG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AGCTGCACCA AAGAGTGAAT GATTTCCGGG ACCGCCTGTG GGACATTTGC
    GATGCCCGCA AGGAAGAAGC AGAGCAGGAG CGTCTGGACA TCATTAACGA GAGCTGGTTG
    CAGGACTCGA CGGGAATTGC CATGAACCAT TTCTTCTCAC TGATGCAGGC AGAACTGAAC
    CGTTTCCAAG ATACGAAGAG ACTCCTGCAA GATTATTACA GGGCCATGGA GTGTAAAATT
    CCAATGGATG ACACTAAGAG ATTCACTCGA GTCCCACTGG TCCAACTGGA TAGTAAAGAT
    ATTTTGGAAA GCCAGCTTAG AATTCCCCTG GTTCCTCGAA GATCCATTTC TCCAGAGTCA
    GCTTTATCCA AACCAAAAAC AAAAAGCATC CTGAAGGGCA AGATCGATTA CATGCTGGAA
    AGTGTAGAGT TAAACTTTGA GGCTGATGAG AAGATGGTGA TGGATACGTG GCAGCAGTCT
    TCTTTGGCGA TATCTCACAT GGTGGCTGCT GAAATTCACC AGAGGCTCAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA AACAAAAGAA AAACTTCCTC AGGCGGCTGC AAATAAAAAA
    GTGAAAAAGG AGCCTCCGCC ACCACCACCA CCACCCAAGA AAAAAAAAGA GGACAAAAAA
    GGAAAAGGTA AATCCCCACC TATGACAGAA GCTGCTCCTG TAGTATTGCC AGCAGAAGAG
    ACTGAGGAAG TGGAAGTGAA AAACGAACTG AAGCTCAGAA TAAAGGAAGA GCATCTTGCT
    GCCCTGCAAT TTGAAGAGAT AGCTACACAG TTCCGACTTG AACTGATAAA GGTAAAAGCA
    TTGGCTTTCC TGGAAGAGTT AGTAATAAAG GCTGTTGATG TATATAGACT CATGGAAAAG
    TGGCTCGGTG AGAGGTACTT GAATGAAATG GCCAGTGTGG AAAAACTAAC TGGGGTAGCT
    CGCTATCACA TTGAAACATC AACCAAAATT CAGAATGAAC TTTATTTAGA CCAAGAAGAC
    TTCTTCATTA ATGGCGATAT CAAAGTCTTT CCAGATCCAC CTCCTCCGGT ACGCCCTCCA
    CCTGTGGAAA AGGAGGAAAA CGGCACCCTG ACCATCGAGC AGCTGGACAA CCTCCGGGAT
    CAGTTTTTAG ATATAGCGCC CAAAGGTATA ATAGGAAATA AAGCATTTGC TGACCTTCTG
    CTGGATTTGG TAACCCTGAA CCTTGGAACA AACAACTTTC CTAGTAGTTG GATGAACCTC
    ACTCAACCTG AATTGCAGGA GTTAGCATCT TTGTTGGTAA CAAACTCGGA GTCCGTGGAC
    TGGCGGAAAT TCTTGTTAGT AGTAGCGCTG CCTTGGCCCA TCCCCCTGGA GGAGGAGCTT
    CTGGAGACCC TGCAGAGGTT CAAGGCCCTG GATGCGGAGC AGCTGGGCAC CATCACTTAC
    GAACAGTATA AGCAGGCTGG CCTGTGGTTT ACAGGAGACG AAGATATCAA AATTCCAGAA
    AATCCTCTTG AACCCCTGCC ATTCAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    TTGTTTGCTG ACTGTGAGAA GGATCCACCC CAACTTGACT ACACGCAGAT GCTGCTTTAT
    TTTGCTTGCC ATCCAGACAG TACAGAAGGG GTCTACAGGG CCCTCAGCGT AGCCATTGGG
    ACTCACGTCT TCCAGCCAAT TGAAATGCCT CATCCTGTTG CAGAAAAGAC CTCTTCCTTT
    ACTGATATGA GTCCCATTGA GGAATATCCT GAACTTGAGG ACAACTTTAT GAGTGAGGAA
    AGGGAATTAC AAGAGGATGG GGAGGAAAAG GAAGAAGAAA TCCCTGAAAA TGCAAATACG
    GAAATGATTT CCATGCAAAC ATTACTCCAA GTGTTCCGAG GAGGAAATGA AGTACTGGAC
    GCTAACAGAT TTGCCAGCCA CCAAAAGTTA GAGAACACTT ATTTAGAGAA CTTCATAAAA
    GTGTTTCAGG ACCTAGGTTC CAAGAACTTA GAGCCAATTG AAGTTGCTGT TCTTCTGAAG
    CATCCTTTTA TTCAAGACCT GATTTCAAGT TATCCAGATT ATAAGATCCC TGATGTTAAG
    ATAATTCTCC AAAGGAGTGA GCATGTACAA GGAAGTGATG AAGAGAGGTC TCCTTCAAGA
    CTTACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94132
    Clone ID Related Accession (Same CDS sequence) XM_015468369.1 , XM_015459107.1
    Accession Version XM_015459107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGGTTCTC CTAGAGGAAA ATCGCTAGGA
    GGGAAGATTC CACTCAAGAA ATCACCTGCC GAGTCTACAG ATGTTTCACC GGTCCCAGTA
    GTGCCAGCCC CATCGAAGCC CGGATCAGAA GAGTGGGTGT ATGTAAATGA ACCAATTCCT
    GAGGAAATAC CATCATTTTT AGTACCATAC TGGAAACTGA TAGAAAATTC CTATATAAAT
    TCCATCAAAA CAGTACTCAG GCAGTTGAGA GAAGACCAGC ATGCCGTACT TGCTTACTTA
    TATGATATTA GAACAGGCTT CCAGCAGTTT CTGAGGCGTC CAGACCACAA GCAAGACTTC
    GTGTCTCAGT GGCAGGCGGA TTTCAACTCC CTTCCTGACG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AGCTGCACCA AAGAGTGAAT GATTTCCGGG ACCGCCTGTG GGACATTTGC
    GATGCCCGCA AGGAAGAAGC AGAGCAGGAG CGTCTGGACA TCATTAACGA GAGCTGGTTG
    CAGGACTCGA CGGGAATTGC CATGAACCAT TTCTTCTCAC TGATGCAGGC AGAACTGAAC
    CGTTTCCAAG ATACGAAGAG ACTCCTGCAA GATTATTACA GGGCCATGGA GTGTAAAATT
    CCAATGGATG ACACTAAGAG ATTCACTCGA GTCCCACTGG TCCAACTGGA TAGTAAAGAT
    ATTTTGGAAA GCCAGCTTAG AATTCCCCTG GTTCCTCGAA GATCCATTTC TCCAGAGTCA
    GCTTTATCCA AACCAAAAAC AAAAAGCATC CTGAAGGGCA AGATCGATTA CATGCTGGAA
    AGTGTAGAGT TAAACTTTGA GGCTGATGAG AAGATGGTGA TGGATACGTG GCAGCAGTCT
    TCTTTGGCGA TATCTCACAT GGTGGCTGCT GAAATTCACC AGAGGCTCAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA AACAAAAGAA AAACTTCCTC AGGCGGCTGC AAATAAAAAA
    GTGAAAAAGG AGCCTCCGCC ACCACCACCA CCACCCAAGA AAAAAAAAGA GGACAAAAAA
    GGAAAAGGTA AATCCCCACC TATGACAGAA GCTGCTCCTG TAGTATTGCC AGCAGAAGAG
    ACTGAGGAAG TGGAAGTGAA AAACGAACTG AAGCTCAGAA TAAAGGAAGA GCATCTTGCT
    GCCCTGCAAT TTGAAGAGAT AGCTACACAG TTCCGACTTG AACTGATAAA GGTAAAAGCA
    TTGGCTTTCC TGGAAGAGTT AGTAATAAAG GCTGTTGATG TATATAGACT CATGGAAAAG
    TGGCTCGGTG AGAGGTACTT GAATGAAATG GCCAGTGTGG AAAAACTAAC TGGGGTAGCT
    CGCTATCACA TTGAAACATC AACCAAAATT CAGAATGAAC TTTATTTAGA CCAAGAAGAC
    TTCTTCATTA ATGGCGATAT CAAAGTCTTT CCAGATCCAC CTCCTCCGGT ACGCCCTCCA
    CCTGTGGAAA AGGAGGAAAA CGGCACCCTG ACCATCGAGC AGCTGGACAA CCTCCGGGAT
    CAGTTTTTAG ATATAGCGCC CAAAGGTATA ATAGGAAATA AAGCATTTGC TGACCTTCTG
    CTGGATTTGG TAACCCTGAA CCTTGGAACA AACAACTTTC CTAGTAGTTG GATGAACCTC
    ACTCAACCTG AATTGCAGGA GTTAGCATCT TTGTTGGTAA CAAACTCGGA GTCCGTGGAC
    TGGCGGAAAT TCTTGTTAGT AGTAGCGCTG CCTTGGCCCA TCCCCCTGGA GGAGGAGCTT
    CTGGAGACCC TGCAGAGGTT CAAGGCCCTG GATGCGGAGC AGCTGGGCAC CATCACTTAC
    GAACAGTATA AGCAGGCTGG CCTGTGGTTT ACAGGAGACG AAGATATCAA AATTCCAGAA
    AATCCTCTTG AACCCCTGCC ATTCAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    TTGTTTGCTG ACTGTGAGAA GGATCCACCC CAACTTGACT ACACGCAGAT GCTGCTTTAT
    TTTGCTTGCC ATCCAGACAG TACAGAAGGG GTCTACAGGG CCCTCAGCGT AGCCATTGGG
    ACTCACGTCT TCCAGCCAAT TGAAATGCCT CATCCTGTTG CAGAAAAGAC CTCTTCCTTT
    ACTGATATGA GTCCCATTGA GGAATATCCT GAACTTGAGG ACAACTTTAT GAGTGAGGAA
    AGGGAATTAC AAGAGGATGG GGAGGAAAAG GAAGAAGAAA TCCCTGAAAA TGCAAATACG
    GAAATGATTT CCATGCAAAC ATTACTCCAA GTGTTCCGAG GAGGAAATGA AGTACTGGAC
    GCTAACAGAT TTGCCAGCCA CCAAAAGTTA GAGAACACTT ATTTAGAGAA CTTCATAAAA
    GTGTTTCAGG ACCTAGGTTC CAAGAACTTA GAGCCAATTG AAGTTGCTGT TCTTCTGAAG
    CATCCTTTTA TTCAAGACCT GATTTCAAGT TATCCAGATT ATAAGATCCC TGATGTTAAG
    ATAATTCTCC AAAGGAGTGA GCATGTACAA GGAAGTGATG AAGAGAGGTC TCCTTCAAGA
    CTTACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314593.1
    CDS114..5354
    Translation

    Target ORF information:

    RefSeq Version XM_015459107.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGGTTCTC CTAGAGGAAA ATCGCTAGGA
    GGGAAGATTC CACTCAAGAA ATCACCTGCC GAGTCTACAG ATGTTTCACC GGTCCCAGTA
    GTGCCAGCCC CATCGAAGCC CGGATCAGAA GAGTGGGTGT ATGTAAATGA ACCAATTCCT
    GAGGAAATAC CATCATTTTT AGTACCATAC TGGAAACTGA TAGAAAATTC CTATATAAAT
    TCCATCAAAA CAGTACTCAG GCAGTTGAGA GAAGACCAGC ATGCCGTACT TGCTTACTTA
    TATGATATTA GAACAGGCTT CCAGCAGTTT CTGAGGCGTC CAGACCACAA GCAAGACTTC
    GTGTCTCAGT GGCAGGCGGA TTTCAACTCC CTTCCTGACG ACCTGTGGGA TGATGAGGAA
    ACAAAGGCTG AGCTGCACCA AAGAGTGAAT GATTTCCGGG ACCGCCTGTG GGACATTTGC
    GATGCCCGCA AGGAAGAAGC AGAGCAGGAG CGTCTGGACA TCATTAACGA GAGCTGGTTG
    CAGGACTCGA CGGGAATTGC CATGAACCAT TTCTTCTCAC TGATGCAGGC AGAACTGAAC
    CGTTTCCAAG ATACGAAGAG ACTCCTGCAA GATTATTACA GGGCCATGGA GTGTAAAATT
    CCAATGGATG ACACTAAGAG ATTCACTCGA GTCCCACTGG TCCAACTGGA TAGTAAAGAT
    ATTTTGGAAA GCCAGCTTAG AATTCCCCTG GTTCCTCGAA GATCCATTTC TCCAGAGTCA
    GCTTTATCCA AACCAAAAAC AAAAAGCATC CTGAAGGGCA AGATCGATTA CATGCTGGAA
    AGTGTAGAGT TAAACTTTGA GGCTGATGAG AAGATGGTGA TGGATACGTG GCAGCAGTCT
    TCTTTGGCGA TATCTCACAT GGTGGCTGCT GAAATTCACC AGAGGCTCAT GGAAGAAGAA
    AAAGAAAACC AGCCAGCAGA AACAAAAGAA AAACTTCCTC AGGCGGCTGC AAATAAAAAA
    GTGAAAAAGG AGCCTCCGCC ACCACCACCA CCACCCAAGA AAAAAAAAGA GGACAAAAAA
    GGAAAAGGTA AATCCCCACC TATGACAGAA GCTGCTCCTG TAGTATTGCC AGCAGAAGAG
    ACTGAGGAAG TGGAAGTGAA AAACGAACTG AAGCTCAGAA TAAAGGAAGA GCATCTTGCT
    GCCCTGCAAT TTGAAGAGAT AGCTACACAG TTCCGACTTG AACTGATAAA GGTAAAAGCA
    TTGGCTTTCC TGGAAGAGTT AGTAATAAAG GCTGTTGATG TATATAGACT CATGGAAAAG
    TGGCTCGGTG AGAGGTACTT GAATGAAATG GCCAGTGTGG AAAAACTAAC TGGGGTAGCT
    CGCTATCACA TTGAAACATC AACCAAAATT CAGAATGAAC TTTATTTAGA CCAAGAAGAC
    TTCTTCATTA ATGGCGATAT CAAAGTCTTT CCAGATCCAC CTCCTCCGGT ACGCCCTCCA
    CCTGTGGAAA AGGAGGAAAA CGGCACCCTG ACCATCGAGC AGCTGGACAA CCTCCGGGAT
    CAGTTTTTAG ATATAGCGCC CAAAGGTATA ATAGGAAATA AAGCATTTGC TGACCTTCTG
    CTGGATTTGG TAACCCTGAA CCTTGGAACA AACAACTTTC CTAGTAGTTG GATGAACCTC
    ACTCAACCTG AATTGCAGGA GTTAGCATCT TTGTTGGTAA CAAACTCGGA GTCCGTGGAC
    TGGCGGAAAT TCTTGTTAGT AGTAGCGCTG CCTTGGCCCA TCCCCCTGGA GGAGGAGCTT
    CTGGAGACCC TGCAGAGGTT CAAGGCCCTG GATGCGGAGC AGCTGGGCAC CATCACTTAC
    GAACAGTATA AGCAGGCTGG CCTGTGGTTT ACAGGAGACG AAGATATCAA AATTCCAGAA
    AATCCTCTTG AACCCCTGCC ATTCAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG
    TTGTTTGCTG ACTGTGAGAA GGATCCACCC CAACTTGACT ACACGCAGAT GCTGCTTTAT
    TTTGCTTGCC ATCCAGACAG TACAGAAGGG GTCTACAGGG CCCTCAGCGT AGCCATTGGG
    ACTCACGTCT TCCAGCCAAT TGAAATGCCT CATCCTGTTG CAGAAAAGAC CTCTTCCTTT
    ACTGATATGA GTCCCATTGA GGAATATCCT GAACTTGAGG ACAACTTTAT GAGTGAGGAA
    AGGGAATTAC AAGAGGATGG GGAGGAAAAG GAAGAAGAAA TCCCTGAAAA TGCAAATACG
    GAAATGATTT CCATGCAAAC ATTACTCCAA GTGTTCCGAG GAGGAAATGA AGTACTGGAC
    GCTAACAGAT TTGCCAGCCA CCAAAAGTTA GAGAACACTT ATTTAGAGAA CTTCATAAAA
    GTGTTTCAGG ACCTAGGTTC CAAGAACTTA GAGCCAATTG AAGTTGCTGT TCTTCTGAAG
    CATCCTTTTA TTCAAGACCT GATTTCAAGT TATCCAGATT ATAAGATCCC TGATGTTAAG
    ATAATTCTCC AAAGGAGTGA GCATGTACAA GGAAGTGATG AAGAGAGGTC TCCTTCAAGA
    CTTACAGAGG AAAAGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94133
    Clone ID Related Accession (Same CDS sequence) XM_015468370.1 , XM_015468372.1 , XM_015459109.1 , XM_015459110.1 , XM_015459108.1 , XM_015468371.1
    Accession Version XM_015468370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323856.1
    CDS439..5406
    Translation

    Target ORF information:

    RefSeq Version XM_015468370.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94133
    Clone ID Related Accession (Same CDS sequence) XM_015468370.1 , XM_015468372.1 , XM_015459109.1 , XM_015459110.1 , XM_015459108.1 , XM_015468371.1
    Accession Version XM_015468372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323858.1
    CDS309..5276
    Translation

    Target ORF information:

    RefSeq Version XM_015468372.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94133
    Clone ID Related Accession (Same CDS sequence) XM_015468370.1 , XM_015468372.1 , XM_015459109.1 , XM_015459110.1 , XM_015459108.1 , XM_015468371.1
    Accession Version XM_015459109.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314595.1
    CDS313..5280
    Translation

    Target ORF information:

    RefSeq Version XM_015459109.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459109.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94133
    Clone ID Related Accession (Same CDS sequence) XM_015468370.1 , XM_015468372.1 , XM_015459109.1 , XM_015459110.1 , XM_015459108.1 , XM_015468371.1
    Accession Version XM_015459110.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314596.1
    CDS309..5276
    Translation

    Target ORF information:

    RefSeq Version XM_015459110.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459110.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94133
    Clone ID Related Accession (Same CDS sequence) XM_015468370.1 , XM_015468372.1 , XM_015459109.1 , XM_015459110.1 , XM_015459108.1 , XM_015468371.1
    Accession Version XM_015459108.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314594.1
    CDS439..5406
    Translation

    Target ORF information:

    RefSeq Version XM_015459108.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94133
    Clone ID Related Accession (Same CDS sequence) XM_015468370.1 , XM_015468372.1 , XM_015459109.1 , XM_015459110.1 , XM_015459108.1 , XM_015468371.1
    Accession Version XM_015468371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4968bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323857.1
    CDS313..5280
    Translation

    Target ORF information:

    RefSeq Version XM_015468371.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGTATAATA
    GGAAATAAAG CATTTGCTGA CCTTCTGCTG GATTTGGTAA CCCTGAACCT TGGAACAAAC
    AACTTTCCTA GTAGTTGGAT GAACCTCACT CAACCTGAAT TGCAGGAGTT AGCATCTTTG
    TTGGTAACAA ACTCGGAGTC CGTGGACTGG CGGAAATTCT TGTTAGTAGT AGCGCTGCCT
    TGGCCCATCC CCCTGGAGGA GGAGCTTCTG GAGACCCTGC AGAGGTTCAA GGCCCTGGAT
    GCGGAGCAGC TGGGCACCAT CACTTACGAA CAGTATAAGC AGGCTGGCCT GTGGTTTACA
    GGAGACGAAG ATATCAAAAT TCCAGAAAAT CCTCTTGAAC CCCTGCCATT CAATAGGCAG
    GAGCATCTTA TAGAGTTTTT CTTTAGGTTG TTTGCTGACT GTGAGAAGGA TCCACCCCAA
    CTTGACTACA CGCAGATGCT GCTTTATTTT GCTTGCCATC CAGACAGTAC AGAAGGGGTC
    TACAGGGCCC TCAGCGTAGC CATTGGGACT CACGTCTTCC AGCCAATTGA AATGCCTCAT
    CCTGTTGCAG AAAAGACCTC TTCCTTTACT GATATGAGTC CCATTGAGGA ATATCCTGAA
    CTTGAGGACA ACTTTATGAG TGAGGAAAGG GAATTACAAG AGGATGGGGA GGAAAAGGAA
    GAAGAAATCC CTGAAAATGC AAATACGGAA ATGATTTCCA TGCAAACATT ACTCCAAGTG
    TTCCGAGGAG GAAATGAAGT ACTGGACGCT AACAGATTTG CCAGCCACCA AAAGTTAGAG
    AACACTTATT TAGAGAACTT CATAAAAGTG TTTCAGGACC TAGGTTCCAA GAACTTAGAG
    CCAATTGAAG TTGCTGTTCT TCTGAAGCAT CCTTTTATTC AAGACCTGAT TTCAAGTTAT
    CCAGATTATA AGATCCCTGA TGTTAAGATA ATTCTCCAAA GGAGTGAGCA TGTACAAGGA
    AGTGATGAAG AGAGGTCTCC TTCAAGACTT ACAGAGGAAA AGAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30690
    Clone ID Related Accession (Same CDS sequence) XM_015468367.1 , XM_002696381.5 , XM_002696381.4
    Accession Version XM_015468367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTGATG TTAAGATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGAAGAG
    AGGTCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323853.1
    CDS114..5429
    Translation

    Target ORF information:

    RefSeq Version XM_015468367.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTGATG TTAAGATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGAAGAG
    AGGTCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30690
    Clone ID Related Accession (Same CDS sequence) XM_015468367.1 , XM_002696381.5 , XM_002696381.4
    Accession Version XM_002696381.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696381.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTGATG TTAAGATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGAAGAG
    AGGTCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696427.2
    CDS391..5706
    Translation

    Target ORF information:

    RefSeq Version XM_002696381.5
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696381.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTGATG TTAAGATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGAAGAG
    AGGTCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30690
    Clone ID Related Accession (Same CDS sequence) XM_015468367.1 , XM_002696381.5 , XM_002696381.4
    Accession Version XM_002696381.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696381.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTGATG TTAAGATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGAAGAG
    AGGTCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002696427.2
    CDS114..5429
    Translation

    Target ORF information:

    RefSeq Version XM_002696381.4
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002696381.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTGATG TTAAGATAAT TCTCCAAAGG AGTGAGCATG TACAAGGAAG TGATGAAGAG
    AGGTCTCCTT CAAGACTTAC AGAGGAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60102
    Clone ID Related Accession (Same CDS sequence) XM_015468368.1 , XM_010816825.2 , XM_010816825.3
    Accession Version XM_015468368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646501.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTAGAC GATTCTATCT GGCACGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323854.1
    CDS113..5365
    Translation

    Target ORF information:

    RefSeq Version XM_015468368.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015468368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTAGAC GATTCTATCT GGCACGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60102
    Clone ID Related Accession (Same CDS sequence) XM_015468368.1 , XM_010816825.2 , XM_010816825.3
    Accession Version XM_010816825.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104515.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTAGAC GATTCTATCT GGCACGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815127.1
    CDS113..5365
    Translation

    Target ORF information:

    RefSeq Version XM_010816825.2
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816825.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTAGAC GATTCTATCT GGCACGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa60102
    Clone ID Related Accession (Same CDS sequence) XM_015468368.1 , XM_010816825.2 , XM_010816825.3
    Accession Version XM_010816825.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTAGAC GATTCTATCT GGCACGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010815127.1
    CDS390..5642
    Translation

    Target ORF information:

    RefSeq Version XM_010816825.3
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010816825.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GTATAATAGG AAATAAAGCA
    TTTGCTGACC TTCTGCTGGA TTTGGTAACC CTGAACCTTG GAACAAACAA CTTTCCTAGT
    AGTTGGATGA ACCTCACTCA ACCTGAATTG CAGGAGTTAG CATCTTTGTT GGTAACAAAC
    TCGGAGTCCG TGGACTGGCG GAAATTCTTG TTAGTAGTAG CGCTGCCTTG GCCCATCCCC
    CTGGAGGAGG AGCTTCTGGA GACCCTGCAG AGGTTCAAGG CCCTGGATGC GGAGCAGCTG
    GGCACCATCA CTTACGAACA GTATAAGCAG GCTGGCCTGT GGTTTACAGG AGACGAAGAT
    ATCAAAATTC CAGAAAATCC TCTTGAACCC CTGCCATTCA ATAGGCAGGA GCATCTTATA
    GAGTTTTTCT TTAGGTTGTT TGCTGACTGT GAGAAGGATC CACCCCAACT TGACTACACG
    CAGATGCTGC TTTATTTTGC TTGCCATCCA GACAGTACAG AAGGGGTCTA CAGGGCCCTC
    AGCGTAGCCA TTGGGACTCA CGTCTTCCAG CCAATTGAAA TGCCTCATCC TGTTGCAGAA
    AAGACCTCTT CCTTTACTGA TATGAGTCCC ATTGAGGAAT ATCCTGAACT TGAGGACAAC
    TTTATGAGTG AGGAAAGGGA ATTACAAGAG GATGGGGAGG AAAAGGAAGA AGAAATCCCT
    GAAAATGCAA ATACGGAAAT GATTTCCATG CAAACATTAC TCCAAGTGTT CCGAGGAGGA
    AATGAAGTAC TGGACGCTAA CAGATTTGCC AGCCACCAAA AGTTAGAGAA CACTTATTTA
    GAGAACTTCA TAAAAGTGTT TCAGGACCTA GGTTCCAAGA ACTTAGAGCC AATTGAAGTT
    GCTGTTCTTC TGAAGCATCC TTTTATTCAA GACCTGATTT CAAGTTATCC AGATTATAAG
    ATCCCTAGAC GATTCTATCT GGCACGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228153
    Clone ID Related Accession (Same CDS sequence) XM_024981501.1
    Accession Version XM_024981501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6372bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GCAGGGATTC TCCAGACGAT
    GTGAGTGGAT TCACCAAGAA GCAGAAAAGG CCTCGGAAGC TTGACCATTA CCTCCCAAAG
    AAGAACAATC AATTTTTGAA AATTTGGCTT AAAAAATACC CTTGGCTTGT ATACGATGAG
    ATACTAAATC TTATGTTCTG TGGCCTGTGT CGTAAACATG GAGTGACATC AAAGGGAAAT
    CAGGTGAGTT TTTTTTATGG CACGGATAAC TTCAGGACTG AATTTCTCAA CGCGCATCAT
    CTGAGCGAGC CTCACGCCAA GGCGTCCCTC ATGGAAGCCA CGAGTGGTTC TCCAGGGAGC
    AGAGCAACCA CGGAGCTGAT GGTGAGGACC ATGAGCAAGG TGACCCTCGG CAGGGTGGAG
    AACCTGTTCC GGTCCTGCCA CGCCATCGCC AAAACCGGAC GTCCCCTCAA AGACTTCCTC
    TGGATGTGTT CGCTGGACGA TATGAAGGGA GTTGATATTG GCCCGGTGTT CAGAACCAGT
    AAATCGGCCA GAACTTTTAC GTACTTTATT GCTGAAGTGG AACGGAGAGC TCTAAGAGAG
    AAGTTAGAAA AAAGTCAGTT CTTCTCCGTC CTCAGTGATG GGATTGCAGC CAGTTCAGTT
    GAGGAAGCTG AACTCGTATA TGTGCAGTTT GCACGCGCAG GGAGAGTGCA CTGCCAAATC
    GTCGGGGTGC AGACCGTGGA GAAGAAGGAG CCCCGGGCGA TAAAACATGC CATCGAGAAA
    ACTCTAGAGA GAAATCTTCA ACTTCGGCTG TCCAACCAAG ACTGGGCAAA GAAACTGGTG
    GGTTTTGGAA GTGATGGAGC TCCGGGTTCA GAGGGAGAAA ACAGCAGTGA GGTGGCCCTG
    CTTTTAAGGG AAATCCAGCC AGGTGTGCTG CCTGTTTACT GCTTTGCCCA TCACCTCGAA
    CTATCTTACC AGGCGGTGTT CCGGAACGTT CCCCTCTACA ACCATGTGAA AGACCTTCTT
    CATAGCATGT ACTGCTTCTA TCACGATTCT CCTGCTCACA GGAGTGCTCT GCTAACTGCT
    TTCAAGGGCC TCCACCTTCG ACCTGCAATG CCCTCTCAAG TCAGCGGCCA GAGGTGGCTT
    CAGGGATTGC AGACTGCCCT CCAGAACTTT CTCAAGGGAT ATCCAGCAAT TGTCCAGCAG
    CTGCATTCAG CAGAAGAAAG CAGAAGTGAC AGCAGTCATC AGAAAGCCAA AGAACTCCTG
    GAGTCTCTCC TCCAGGCAGA CGTCGTCAAA TTTGTCCACT TCTTGTTGGA TGTCGTCAAT
    GTCCTTAGCA TACTGTCCCA CGTCAGTCAG AACAGAAATT CCTCAATTGC TGATATATTT
    GCCACCTTAG AGTCAACACT GGAAATGCTC CAAATCTATC AGACAAGACC AGGGCCGAAA
    GAACGTGGGG TGGATTCAGT CACACACTTT CATGGTAACT GCCTAGGAGG AAAGGGAAAC
    ATTTCTGATG TGAGAAACAT GGTTTTAACA CATCTTATCA AGAAGCTGCG AGGCTGTTTC
    AGAGATGCCA ACCAAGATGT GGTGAAGGCA ACCATGATTG GAAGCTTTAA ACTGTGGCCG
    GCAAAAATAA ATCAAGAATT TGGAGAAAAA GAGGTGGCCA TCCTGATTGC ACATTATGAA
    CCAGTATTAG AGGCAGCCAA GGTGAAAATT GACCAAGTAG ACACAGAATG GAGCATGTTG
    AAATTGGAGA TCTACTCTAG ATTTCAGAAC ATTCGCAAAC TGACCTGGGA TTTTGTGAAT
    TCTGTTTATT TACACAAGTA TCCAAACATC CTGACACTCA TTGACCTCGT GCTGAGCCTG
    CCTGCAAGTT CAGCTGAGGC AGGACGCGGC TTTAGTCAGA TGAAGCGCAT CAGGTCCCAA
    ATGCATGCCA AGGTCAAGGC TGACAGCATG ACGGATCTCC TGATCATTCA GTTGAATTCT
    CCAGACATTA ACAACTTTGA CCCTAGGAAA GCCATTCATT TATGGAATAC AAGAACACCA
    TCCTCAACTG AGGACACAAG ACCTAACTCA GATTGCTCAT CAAACTCAGA AAGCCTTGGG
    GAAAGTGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837269.1
    CDS404..6775
    Translation

    Target ORF information:

    RefSeq Version XM_024981501.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAACTGA AGGTGTCCCA GACCGTGAGT 
    CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAA TTGGAGAAGT TTTGCACAAG
    TTTGCACTTC AGGATGATTT TGCAGACTTT TCAGAAAGCA GGATTTCCAG TGCCAAACTT
    AACAATTTTT CTCGCTTGGA GCCCACACTT CACCTTTTGG GTGTGCAATT TGATCAGAAT
    GTGGCCCACA ATATCATCAC AGAGAAGCCT GGGGCTGCCA CGAAACTCTT ATATCAGCTG
    TACATTGCTC TCCAGAGGAA AAAGAAAGGT GTTCAGACTG GAGTGGAAAT GCCAACTGTG
    CAGCCCCTGA TGAGCACAAA AGTTCAAAAC ATGAAAAGCG AGGCTTTTCG AGATCAACGC
    TTGAGCAGAA GACGACAAAA TGAAATCATG GCCAAAATCC AAGCAGCTAT CATACAGATT
    CCTAAACCCA CATCAAATCG TACTCTGAAA ACCCTTGATG CCCAAAAATT AATGAAAAAG
    AAAAAAGAGG CAGAGGATGT GGCCAATGAA ATTAAGAAGT TTGAAGCATT AATAAAAAAG
    GATCTCCAGG CAAAAGAAAG TGCATCCAAG ACTTCTCTAG ATACAGCAGG CCAGACGACC
    ACTGATTTAT TAAACACTTA TTCAGATGAT GACTACATTA AAAAAATCCA AAAACGCCTT
    GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAGACT CTTAATGGAT
    CAGTTAATAG CTCATGAAGC CCAAGAGGAG GCTTATCGGG AGGAGCAGTT GATCAACCGG
    CTGATGCGAC AGTCCCAGCA GGAACGCAGG ATCGCTGTGC AGCTCATGCA TGTTCGGCAC
    GAAAAGGAAG TTTTGTGGCA AAACAGAATT TTCAGAGAAA AACAATATGA GGAAAGACGG
    CTTAAAGACT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TAGCCAGACA AGCCAAGATT
    GATTTTGAAG AACAAGCCCT CAGAGATAAG GAAATTCATG AGCAGATCGC CATAGAAAGA
    GCTCAAGCTC GTTACGAAAA GCATTACTCG ATATGTGCAG AAATTTTGGA TCAGATACTT
    GATTTGTCCA CTAAGGTGGC AGACTATCGA TTGTTGACAA ATAACCTGAT CCCATATAAG
    TTGATGCATG ATTGGAAGGA ACTGTTTTTT CATGGAAAAC CCATATATGA GCAAGCCTCT
    CTTAGACATT TACCAACTAA GCTCTCTGCA GAACACCTTA TAGAACTGGA GAAAAGGAAC
    TTGCTAGATA ACAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAATG GGCCTTACCA
    GAAGAAATGG TTGAAAACTT ACCACCCTCC AACAATTGCA TATTGGGCCA TGTGATTCAC
    AGACTAATTG AAAGTTCTCT TCCTCCTCAA GTTGAATCTA CAGTACCTGA GTTACCTTCA
    TTTGCTATAA AAGGATGCTT GTTGGGGAAA ACCTTTAGTG GAAAAACTAC TGCTCTAAAG
    GCTCTAGAGA AAAGCTTTCC TATTCAGGTA CTTTCTATTG ACACTCTTGT TCAAGAAGCT
    ATCCAAGCAT TTCATGACAA AGAAAGTGTC AGCGGGGCCC ACGGACTTCA GAAAGCACCT
    GAAGAAAAGG CTTTACCAGT TAGGCAAGAG AGTGATGAGG ACAACCAGGC ATGTACTTCC
    TCCCCAAAGG ACGTGCAAGA TGGATCTTTA GCTGAAGCAC TACCAAAAAA AGAAGATAAA
    ACCAGACTTG ATACCGATGC TAGAAAAGCA GCAAAAGCTG AAGAAGTCGT AGCGAGTGAT
    AGTTTCTCTG AACTCACTGT GCGTGCTCAG CTTGGTGCAA AATCAGAAAG GCTGCTGAAG
    AAAGGAAAGA GCATATCTGA TATATTGCTA GTCAGCATCA TGGTAAATGC CATTAATCAG
    ATACCTATGT ATAAAGGTTG GATCCTGGAT GGCTTTCCAA TGACATTAAA CCAAGCAAAG
    CTTTTGGAGG AAGCACTCAC AGGCTACAAC AGAAACCTCA TAGAACTGGA GGGAAAGAAA
    TCACACACAT CCACATTAGC TGTTGACCCT ACAGCTTCCA AAGAAGTGCC TCTTCCCCCT
    TCTGCATTTG ATTTTGTCAT GTTATTAGAC ATTTCAGATA ATTCCTCACT GAATCGCATG
    AATGACATCA TGGCTGAAGC ATTTTCAAGT GAAACTCCTC TTGAAGATAT CAATCAGCGT
    GTAGCTGCTA AAAACAAAGA TACAGATGAG GACCAGAATT TAAGAGATCA AATACAGCAC
    AGAATTGTAG GCTTCCTGGA CAACTGGCCT TCATTGGAGC AGTGGTTTAC AGAGCCAGAA
    AACATTTTGA TAAAAATCAA TGCTGAAGTA GATAAACAGT CATTGTGCCA AAAAGTAAAG
    GAAATGTTTA TGACTGAAAT AATGAAAAAA GAGAATAAAG TGAAAAAGAA ATTAGAAGAA
    AAGGAAGCTG AGAGAAAAGA AGAAGTTTCC CTGACTGAGA AGACATCCCC TCCTCCTCCT
    CCTCCTCCCC CTCCGGAACC AGAGAAAGAG AAAGAAGTCC ACCTTCCACA GGAGCGGTCC
    AAAACTCCTG CTGCGAAAGG AAAGCAAGCA TCAGAACCCC CGCATGGTAA TCGAGAACCT
    CTTCAGGAAG GAAAAGGGAA GAAAAGTGAA ACTTCACTCA AAAGAAAAGG TTCTCCTAGA
    GGAAAATCGC TAGGAGGGAA GATTCCACTC AAGAAATCAC CTGCCGAGTC TACAGATGTT
    TCACCGGTCC CAGTAGTGCC AGCCCCATCG AAGCCCGGAT CAGAAGAGTG GGTGTATGTA
    AATGAACCAA TTCCTGAGGA AATACCATCA TTTTTAGTAC CATACTGGAA ACTGATAGAA
    AATTCCTATA TAAATTCCAT CAAAACAGTA CTCAGGCAGT TGAGAGAAGA CCAGCATGCC
    GTACTTGCTT ACTTATATGA TATTAGAACA GGCTTCCAGC AGTTTCTGAG GCGTCCAGAC
    CACAAGCAAG ACTTCGTGTC TCAGTGGCAG GCGGATTTCA ACTCCCTTCC TGACGACCTG
    TGGGATGATG AGGAAACAAA GGCTGAGCTG CACCAAAGAG TGAATGATTT CCGGGACCGC
    CTGTGGGACA TTTGCGATGC CCGCAAGGAA GAAGCAGAGC AGGAGCGTCT GGACATCATT
    AACGAGAGCT GGTTGCAGGA CTCGACGGGA ATTGCCATGA ACCATTTCTT CTCACTGATG
    CAGGCAGAAC TGAACCGTTT CCAAGATACG AAGAGACTCC TGCAAGATTA TTACAGGGCC
    ATGGAGTGTA AAATTCCAAT GGATGACACT AAGAGATTCA CTCGAGTCCC ACTGGTCCAA
    CTGGATAGTA AAGATATTTT GGAAAGCCAG CTTAGAATTC CCCTGGTTCC TCGAAGATCC
    ATTTCTCCAG AGTCAGCTTT ATCCAAACCA AAAACAAAAA GCATCCTGAA GGGCAAGATC
    GATTACATGC TGGAAAGTGT AGAGTTAAAC TTTGAGGCTG ATGAGAAGAT GGTGATGGAT
    ACGTGGCAGC AGTCTTCTTT GGCGATATCT CACATGGTGG CTGCTGAAAT TCACCAGAGG
    CTCATGGAAG AAGAAAAAGA AAACCAGCCA GCAGAAACAA AAGAAAAACT TCCTCAGGCG
    GCTGCAAATA AAAAAGTGAA AAAGGAGCCT CCGCCACCAC CACCACCACC CAAGAAAAAA
    AAAGAGGACA AAAAAGGAAA AGGTAAATCC CCACCTATGA CAGAAGCTGC TCCTGTAGTA
    TTGCCAGCAG AAGAGACTGA GGAAGTGGAA GTGAAAAACG AACTGAAGCT CAGAATAAAG
    GAAGAGCATC TTGCTGCCCT GCAATTTGAA GAGATAGCTA CACAGTTCCG ACTTGAACTG
    ATAAAGGTAA AAGCATTGGC TTTCCTGGAA GAGTTAGTAA TAAAGGCTGT TGATGTATAT
    AGACTCATGG AAAAGTGGCT CGGTGAGAGG TACTTGAATG AAATGGCCAG TGTGGAAAAA
    CTAACTGGGG TAGCTCGCTA TCACATTGAA ACATCAACCA AAATTCAGAA TGAACTTTAT
    TTAGACCAAG AAGACTTCTT CATTAATGGC GATATCAAAG TCTTTCCAGA TCCACCTCCT
    CCGGTACGCC CTCCACCTGT GGAAAAGGAG GAAAACGGCA CCCTGACCAT CGAGCAGCTG
    GACAACCTCC GGGATCAGTT TTTAGATATA GCGCCCAAAG GCAGGGATTC TCCAGACGAT
    GTGAGTGGAT TCACCAAGAA GCAGAAAAGG CCTCGGAAGC TTGACCATTA CCTCCCAAAG
    AAGAACAATC AATTTTTGAA AATTTGGCTT AAAAAATACC CTTGGCTTGT ATACGATGAG
    ATACTAAATC TTATGTTCTG TGGCCTGTGT CGTAAACATG GAGTGACATC AAAGGGAAAT
    CAGGTGAGTT TTTTTTATGG CACGGATAAC TTCAGGACTG AATTTCTCAA CGCGCATCAT
    CTGAGCGAGC CTCACGCCAA GGCGTCCCTC ATGGAAGCCA CGAGTGGTTC TCCAGGGAGC
    AGAGCAACCA CGGAGCTGAT GGTGAGGACC ATGAGCAAGG TGACCCTCGG CAGGGTGGAG
    AACCTGTTCC GGTCCTGCCA CGCCATCGCC AAAACCGGAC GTCCCCTCAA AGACTTCCTC
    TGGATGTGTT CGCTGGACGA TATGAAGGGA GTTGATATTG GCCCGGTGTT CAGAACCAGT
    AAATCGGCCA GAACTTTTAC GTACTTTATT GCTGAAGTGG AACGGAGAGC TCTAAGAGAG
    AAGTTAGAAA AAAGTCAGTT CTTCTCCGTC CTCAGTGATG GGATTGCAGC CAGTTCAGTT
    GAGGAAGCTG AACTCGTATA TGTGCAGTTT GCACGCGCAG GGAGAGTGCA CTGCCAAATC
    GTCGGGGTGC AGACCGTGGA GAAGAAGGAG CCCCGGGCGA TAAAACATGC CATCGAGAAA
    ACTCTAGAGA GAAATCTTCA ACTTCGGCTG TCCAACCAAG ACTGGGCAAA GAAACTGGTG
    GGTTTTGGAA GTGATGGAGC TCCGGGTTCA GAGGGAGAAA ACAGCAGTGA GGTGGCCCTG
    CTTTTAAGGG AAATCCAGCC AGGTGTGCTG CCTGTTTACT GCTTTGCCCA TCACCTCGAA
    CTATCTTACC AGGCGGTGTT CCGGAACGTT CCCCTCTACA ACCATGTGAA AGACCTTCTT
    CATAGCATGT ACTGCTTCTA TCACGATTCT CCTGCTCACA GGAGTGCTCT GCTAACTGCT
    TTCAAGGGCC TCCACCTTCG ACCTGCAATG CCCTCTCAAG TCAGCGGCCA GAGGTGGCTT
    CAGGGATTGC AGACTGCCCT CCAGAACTTT CTCAAGGGAT ATCCAGCAAT TGTCCAGCAG
    CTGCATTCAG CAGAAGAAAG CAGAAGTGAC AGCAGTCATC AGAAAGCCAA AGAACTCCTG
    GAGTCTCTCC TCCAGGCAGA CGTCGTCAAA TTTGTCCACT TCTTGTTGGA TGTCGTCAAT
    GTCCTTAGCA TACTGTCCCA CGTCAGTCAG AACAGAAATT CCTCAATTGC TGATATATTT
    GCCACCTTAG AGTCAACACT GGAAATGCTC CAAATCTATC AGACAAGACC AGGGCCGAAA
    GAACGTGGGG TGGATTCAGT CACACACTTT CATGGTAACT GCCTAGGAGG AAAGGGAAAC
    ATTTCTGATG TGAGAAACAT GGTTTTAACA CATCTTATCA AGAAGCTGCG AGGCTGTTTC
    AGAGATGCCA ACCAAGATGT GGTGAAGGCA ACCATGATTG GAAGCTTTAA ACTGTGGCCG
    GCAAAAATAA ATCAAGAATT TGGAGAAAAA GAGGTGGCCA TCCTGATTGC ACATTATGAA
    CCAGTATTAG AGGCAGCCAA GGTGAAAATT GACCAAGTAG ACACAGAATG GAGCATGTTG
    AAATTGGAGA TCTACTCTAG ATTTCAGAAC ATTCGCAAAC TGACCTGGGA TTTTGTGAAT
    TCTGTTTATT TACACAAGTA TCCAAACATC CTGACACTCA TTGACCTCGT GCTGAGCCTG
    CCTGCAAGTT CAGCTGAGGC AGGACGCGGC TTTAGTCAGA TGAAGCGCAT CAGGTCCCAA
    ATGCATGCCA AGGTCAAGGC TGACAGCATG ACGGATCTCC TGATCATTCA GTTGAATTCT
    CCAGACATTA ACAACTTTGA CCCTAGGAAA GCCATTCATT TATGGAATAC AAGAACACCA
    TCCTCAACTG AGGACACAAG ACCTAACTCA GATTGCTCAT CAAACTCAGA AAGCCTTGGG
    GAAAGTGACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228154
    Clone ID Related Accession (Same CDS sequence) XM_024981502.1 , XM_024981503.1
    Accession Version XM_024981502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGCAGGGAT
    TCTCCAGACG ATGTGAGTGG ATTCACCAAG AAGCAGAAAA GGCCTCGGAA GCTTGACCAT
    TACCTCCCAA AGAAGAACAA TCAATTTTTG AAAATTTGGC TTAAAAAATA CCCTTGGCTT
    GTATACGATG AGATACTAAA TCTTATGTTC TGTGGCCTGT GTCGTAAACA TGGAGTGACA
    TCAAAGGGAA ATCAGGTGAG TTTTTTTTAT GGCACGGATA ACTTCAGGAC TGAATTTCTC
    AACGCGCATC ATCTGAGCGA GCCTCACGCC AAGGCGTCCC TCATGGAAGC CACGAGTGGT
    TCTCCAGGGA GCAGAGCAAC CACGGAGCTG ATGGTGAGGA CCATGAGCAA GGTGACCCTC
    GGCAGGGTGG AGAACCTGTT CCGGTCCTGC CACGCCATCG CCAAAACCGG ACGTCCCCTC
    AAAGACTTCC TCTGGATGTG TTCGCTGGAC GATATGAAGG GAGTTGATAT TGGCCCGGTG
    TTCAGAACCA GTAAATCGGC CAGAACTTTT ACGTACTTTA TTGCTGAAGT GGAACGGAGA
    GCTCTAAGAG AGAAGTTAGA AAAAAGTCAG TTCTTCTCCG TCCTCAGTGA TGGGATTGCA
    GCCAGTTCAG TTGAGGAAGC TGAACTCGTA TATGTGCAGT TTGCACGCGC AGGGAGAGTG
    CACTGCCAAA TCGTCGGGGT GCAGACCGTG GAGAAGAAGG AGCCCCGGGC GATAAAACAT
    GCCATCGAGA AAACTCTAGA GAGAAATCTT CAACTTCGGC TGTCCAACCA AGACTGGGCA
    AAGAAACTGG TGGGTTTTGG AAGTGATGGA GCTCCGGGTT CAGAGGGAGA AAACAGCAGT
    GAGGTGGCCC TGCTTTTAAG GGAAATCCAG CCAGGTGTGC TGCCTGTTTA CTGCTTTGCC
    CATCACCTCG AACTATCTTA CCAGGCGGTG TTCCGGAACG TTCCCCTCTA CAACCATGTG
    AAAGACCTTC TTCATAGCAT GTACTGCTTC TATCACGATT CTCCTGCTCA CAGGAGTGCT
    CTGCTAACTG CTTTCAAGGG CCTCCACCTT CGACCTGCAA TGCCCTCTCA AGTCAGCGGC
    CAGAGGTGGC TTCAGGGATT GCAGACTGCC CTCCAGAACT TTCTCAAGGG ATATCCAGCA
    ATTGTCCAGC AGCTGCATTC AGCAGAAGAA AGCAGAAGTG ACAGCAGTCA TCAGAAAGCC
    AAAGAACTCC TGGAGTCTCT CCTCCAGGCA GACGTCGTCA AATTTGTCCA CTTCTTGTTG
    GATGTCGTCA ATGTCCTTAG CATACTGTCC CACGTCAGTC AGAACAGAAA TTCCTCAATT
    GCTGATATAT TTGCCACCTT AGAGTCAACA CTGGAAATGC TCCAAATCTA TCAGACAAGA
    CCAGGGCCGA AAGAACGTGG GGTGGATTCA GTCACACACT TTCATGGTAA CTGCCTAGGA
    GGAAAGGGAA ACATTTCTGA TGTGAGAAAC ATGGTTTTAA CACATCTTAT CAAGAAGCTG
    CGAGGCTGTT TCAGAGATGC CAACCAAGAT GTGGTGAAGG CAACCATGAT TGGAAGCTTT
    AAACTGTGGC CGGCAAAAAT AAATCAAGAA TTTGGAGAAA AAGAGGTGGC CATCCTGATT
    GCACATTATG AACCAGTATT AGAGGCAGCC AAGGTGAAAA TTGACCAAGT AGACACAGAA
    TGGAGCATGT TGAAATTGGA GATCTACTCT AGATTTCAGA ACATTCGCAA ACTGACCTGG
    GATTTTGTGA ATTCTGTTTA TTTACACAAG TATCCAAACA TCCTGACACT CATTGACCTC
    GTGCTGAGCC TGCCTGCAAG TTCAGCTGAG GCAGGACGCG GCTTTAGTCA GATGAAGCGC
    ATCAGGTCCC AAATGCATGC CAAGGTCAAG GCTGACAGCA TGACGGATCT CCTGATCATT
    CAGTTGAATT CTCCAGACAT TAACAACTTT GACCCTAGGA AAGCCATTCA TTTATGGAAT
    ACAAGAACAC CATCCTCAAC TGAGGACACA AGACCTAACT CAGATTGCTC ATCAAACTCA
    GAAAGCCTTG GGGAAAGTGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837270.1
    CDS749..6772
    Translation

    Target ORF information:

    RefSeq Version XM_024981502.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGCAGGGAT
    TCTCCAGACG ATGTGAGTGG ATTCACCAAG AAGCAGAAAA GGCCTCGGAA GCTTGACCAT
    TACCTCCCAA AGAAGAACAA TCAATTTTTG AAAATTTGGC TTAAAAAATA CCCTTGGCTT
    GTATACGATG AGATACTAAA TCTTATGTTC TGTGGCCTGT GTCGTAAACA TGGAGTGACA
    TCAAAGGGAA ATCAGGTGAG TTTTTTTTAT GGCACGGATA ACTTCAGGAC TGAATTTCTC
    AACGCGCATC ATCTGAGCGA GCCTCACGCC AAGGCGTCCC TCATGGAAGC CACGAGTGGT
    TCTCCAGGGA GCAGAGCAAC CACGGAGCTG ATGGTGAGGA CCATGAGCAA GGTGACCCTC
    GGCAGGGTGG AGAACCTGTT CCGGTCCTGC CACGCCATCG CCAAAACCGG ACGTCCCCTC
    AAAGACTTCC TCTGGATGTG TTCGCTGGAC GATATGAAGG GAGTTGATAT TGGCCCGGTG
    TTCAGAACCA GTAAATCGGC CAGAACTTTT ACGTACTTTA TTGCTGAAGT GGAACGGAGA
    GCTCTAAGAG AGAAGTTAGA AAAAAGTCAG TTCTTCTCCG TCCTCAGTGA TGGGATTGCA
    GCCAGTTCAG TTGAGGAAGC TGAACTCGTA TATGTGCAGT TTGCACGCGC AGGGAGAGTG
    CACTGCCAAA TCGTCGGGGT GCAGACCGTG GAGAAGAAGG AGCCCCGGGC GATAAAACAT
    GCCATCGAGA AAACTCTAGA GAGAAATCTT CAACTTCGGC TGTCCAACCA AGACTGGGCA
    AAGAAACTGG TGGGTTTTGG AAGTGATGGA GCTCCGGGTT CAGAGGGAGA AAACAGCAGT
    GAGGTGGCCC TGCTTTTAAG GGAAATCCAG CCAGGTGTGC TGCCTGTTTA CTGCTTTGCC
    CATCACCTCG AACTATCTTA CCAGGCGGTG TTCCGGAACG TTCCCCTCTA CAACCATGTG
    AAAGACCTTC TTCATAGCAT GTACTGCTTC TATCACGATT CTCCTGCTCA CAGGAGTGCT
    CTGCTAACTG CTTTCAAGGG CCTCCACCTT CGACCTGCAA TGCCCTCTCA AGTCAGCGGC
    CAGAGGTGGC TTCAGGGATT GCAGACTGCC CTCCAGAACT TTCTCAAGGG ATATCCAGCA
    ATTGTCCAGC AGCTGCATTC AGCAGAAGAA AGCAGAAGTG ACAGCAGTCA TCAGAAAGCC
    AAAGAACTCC TGGAGTCTCT CCTCCAGGCA GACGTCGTCA AATTTGTCCA CTTCTTGTTG
    GATGTCGTCA ATGTCCTTAG CATACTGTCC CACGTCAGTC AGAACAGAAA TTCCTCAATT
    GCTGATATAT TTGCCACCTT AGAGTCAACA CTGGAAATGC TCCAAATCTA TCAGACAAGA
    CCAGGGCCGA AAGAACGTGG GGTGGATTCA GTCACACACT TTCATGGTAA CTGCCTAGGA
    GGAAAGGGAA ACATTTCTGA TGTGAGAAAC ATGGTTTTAA CACATCTTAT CAAGAAGCTG
    CGAGGCTGTT TCAGAGATGC CAACCAAGAT GTGGTGAAGG CAACCATGAT TGGAAGCTTT
    AAACTGTGGC CGGCAAAAAT AAATCAAGAA TTTGGAGAAA AAGAGGTGGC CATCCTGATT
    GCACATTATG AACCAGTATT AGAGGCAGCC AAGGTGAAAA TTGACCAAGT AGACACAGAA
    TGGAGCATGT TGAAATTGGA GATCTACTCT AGATTTCAGA ACATTCGCAA ACTGACCTGG
    GATTTTGTGA ATTCTGTTTA TTTACACAAG TATCCAAACA TCCTGACACT CATTGACCTC
    GTGCTGAGCC TGCCTGCAAG TTCAGCTGAG GCAGGACGCG GCTTTAGTCA GATGAAGCGC
    ATCAGGTCCC AAATGCATGC CAAGGTCAAG GCTGACAGCA TGACGGATCT CCTGATCATT
    CAGTTGAATT CTCCAGACAT TAACAACTTT GACCCTAGGA AAGCCATTCA TTTATGGAAT
    ACAAGAACAC CATCCTCAAC TGAGGACACA AGACCTAACT CAGATTGCTC ATCAAACTCA
    GAAAGCCTTG GGGAAAGTGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa228154
    Clone ID Related Accession (Same CDS sequence) XM_024981502.1 , XM_024981503.1
    Accession Version XM_024981503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product sperm flagellar protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGCAGGGAT
    TCTCCAGACG ATGTGAGTGG ATTCACCAAG AAGCAGAAAA GGCCTCGGAA GCTTGACCAT
    TACCTCCCAA AGAAGAACAA TCAATTTTTG AAAATTTGGC TTAAAAAATA CCCTTGGCTT
    GTATACGATG AGATACTAAA TCTTATGTTC TGTGGCCTGT GTCGTAAACA TGGAGTGACA
    TCAAAGGGAA ATCAGGTGAG TTTTTTTTAT GGCACGGATA ACTTCAGGAC TGAATTTCTC
    AACGCGCATC ATCTGAGCGA GCCTCACGCC AAGGCGTCCC TCATGGAAGC CACGAGTGGT
    TCTCCAGGGA GCAGAGCAAC CACGGAGCTG ATGGTGAGGA CCATGAGCAA GGTGACCCTC
    GGCAGGGTGG AGAACCTGTT CCGGTCCTGC CACGCCATCG CCAAAACCGG ACGTCCCCTC
    AAAGACTTCC TCTGGATGTG TTCGCTGGAC GATATGAAGG GAGTTGATAT TGGCCCGGTG
    TTCAGAACCA GTAAATCGGC CAGAACTTTT ACGTACTTTA TTGCTGAAGT GGAACGGAGA
    GCTCTAAGAG AGAAGTTAGA AAAAAGTCAG TTCTTCTCCG TCCTCAGTGA TGGGATTGCA
    GCCAGTTCAG TTGAGGAAGC TGAACTCGTA TATGTGCAGT TTGCACGCGC AGGGAGAGTG
    CACTGCCAAA TCGTCGGGGT GCAGACCGTG GAGAAGAAGG AGCCCCGGGC GATAAAACAT
    GCCATCGAGA AAACTCTAGA GAGAAATCTT CAACTTCGGC TGTCCAACCA AGACTGGGCA
    AAGAAACTGG TGGGTTTTGG AAGTGATGGA GCTCCGGGTT CAGAGGGAGA AAACAGCAGT
    GAGGTGGCCC TGCTTTTAAG GGAAATCCAG CCAGGTGTGC TGCCTGTTTA CTGCTTTGCC
    CATCACCTCG AACTATCTTA CCAGGCGGTG TTCCGGAACG TTCCCCTCTA CAACCATGTG
    AAAGACCTTC TTCATAGCAT GTACTGCTTC TATCACGATT CTCCTGCTCA CAGGAGTGCT
    CTGCTAACTG CTTTCAAGGG CCTCCACCTT CGACCTGCAA TGCCCTCTCA AGTCAGCGGC
    CAGAGGTGGC TTCAGGGATT GCAGACTGCC CTCCAGAACT TTCTCAAGGG ATATCCAGCA
    ATTGTCCAGC AGCTGCATTC AGCAGAAGAA AGCAGAAGTG ACAGCAGTCA TCAGAAAGCC
    AAAGAACTCC TGGAGTCTCT CCTCCAGGCA GACGTCGTCA AATTTGTCCA CTTCTTGTTG
    GATGTCGTCA ATGTCCTTAG CATACTGTCC CACGTCAGTC AGAACAGAAA TTCCTCAATT
    GCTGATATAT TTGCCACCTT AGAGTCAACA CTGGAAATGC TCCAAATCTA TCAGACAAGA
    CCAGGGCCGA AAGAACGTGG GGTGGATTCA GTCACACACT TTCATGGTAA CTGCCTAGGA
    GGAAAGGGAA ACATTTCTGA TGTGAGAAAC ATGGTTTTAA CACATCTTAT CAAGAAGCTG
    CGAGGCTGTT TCAGAGATGC CAACCAAGAT GTGGTGAAGG CAACCATGAT TGGAAGCTTT
    AAACTGTGGC CGGCAAAAAT AAATCAAGAA TTTGGAGAAA AAGAGGTGGC CATCCTGATT
    GCACATTATG AACCAGTATT AGAGGCAGCC AAGGTGAAAA TTGACCAAGT AGACACAGAA
    TGGAGCATGT TGAAATTGGA GATCTACTCT AGATTTCAGA ACATTCGCAA ACTGACCTGG
    GATTTTGTGA ATTCTGTTTA TTTACACAAG TATCCAAACA TCCTGACACT CATTGACCTC
    GTGCTGAGCC TGCCTGCAAG TTCAGCTGAG GCAGGACGCG GCTTTAGTCA GATGAAGCGC
    ATCAGGTCCC AAATGCATGC CAAGGTCAAG GCTGACAGCA TGACGGATCT CCTGATCATT
    CAGTTGAATT CTCCAGACAT TAACAACTTT GACCCTAGGA AAGCCATTCA TTTATGGAAT
    ACAAGAACAC CATCCTCAAC TGAGGACACA AGACCTAACT CAGATTGCTC ATCAAACTCA
    GAAAGCCTTG GGGAAAGTGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837271.1
    CDS650..6673
    Translation

    Target ORF information:

    RefSeq Version XM_024981503.1
    Organism Bos taurus(cattle)
    Definition Bos taurus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCCAACTG TGCAGCCCCT GATGAGCACA AAAGTTCAAA ACATGAAAAG CGAGGCTTTT 
    CGAGATCAAC GCTTGAGCAG AAGACGACAA AATGAAATCA TGGCCAAAAT CCAAGCAGCT
    ATCATACAGA TTCCTAAACC CACATCAAAT CGTACTCTGA AAACCCTTGA TGCCCAAAAA
    TTAATGAAAA AGAAAAAAGA GGCAGAGGAT GTGGCCAATG AAATTAAGAA GTTTGAAGCA
    TTAATAAAAA AGGATCTCCA GGCAAAAGAA AGTGCATCCA AGACTTCTCT AGATACAGCA
    GGCCAGACGA CCACTGATTT ATTAAACACT TATTCAGATG ATGACTACAT TAAAAAAATC
    CAAAAACGCC TTGAAGAAGA TGCTTTTGCA CGAGAGCAAA GGGAGAAAAG ACGGCGGAGA
    CTCTTAATGG ATCAGTTAAT AGCTCATGAA GCCCAAGAGG AGGCTTATCG GGAGGAGCAG
    TTGATCAACC GGCTGATGCG ACAGTCCCAG CAGGAACGCA GGATCGCTGT GCAGCTCATG
    CATGTTCGGC ACGAAAAGGA AGTTTTGTGG CAAAACAGAA TTTTCAGAGA AAAACAATAT
    GAGGAAAGAC GGCTTAAAGA CTTCCAGGAT GCTCTTGATC GAGAAGCGGC TTTAGCCAGA
    CAAGCCAAGA TTGATTTTGA AGAACAAGCC CTCAGAGATA AGGAAATTCA TGAGCAGATC
    GCCATAGAAA GAGCTCAAGC TCGTTACGAA AAGCATTACT CGATATGTGC AGAAATTTTG
    GATCAGATAC TTGATTTGTC CACTAAGGTG GCAGACTATC GATTGTTGAC AAATAACCTG
    ATCCCATATA AGTTGATGCA TGATTGGAAG GAACTGTTTT TTCATGGAAA ACCCATATAT
    GAGCAAGCCT CTCTTAGACA TTTACCAACT AAGCTCTCTG CAGAACACCT TATAGAACTG
    GAGAAAAGGA ACTTGCTAGA TAACAATGAT TATGAAGAAT ATAAGAACAT GGTTGGAGAA
    TGGGCCTTAC CAGAAGAAAT GGTTGAAAAC TTACCACCCT CCAACAATTG CATATTGGGC
    CATGTGATTC ACAGACTAAT TGAAAGTTCT CTTCCTCCTC AAGTTGAATC TACAGTACCT
    GAGTTACCTT CATTTGCTAT AAAAGGATGC TTGTTGGGGA AAACCTTTAG TGGAAAAACT
    ACTGCTCTAA AGGCTCTAGA GAAAAGCTTT CCTATTCAGG TACTTTCTAT TGACACTCTT
    GTTCAAGAAG CTATCCAAGC ATTTCATGAC AAAGAAAGTG TCAGCGGGGC CCACGGACTT
    CAGAAAGCAC CTGAAGAAAA GGCTTTACCA GTTAGGCAAG AGAGTGATGA GGACAACCAG
    GCATGTACTT CCTCCCCAAA GGACGTGCAA GATGGATCTT TAGCTGAAGC ACTACCAAAA
    AAAGAAGATA AAACCAGACT TGATACCGAT GCTAGAAAAG CAGCAAAAGC TGAAGAAGTC
    GTAGCGAGTG ATAGTTTCTC TGAACTCACT GTGCGTGCTC AGCTTGGTGC AAAATCAGAA
    AGGCTGCTGA AGAAAGGAAA GAGCATATCT GATATATTGC TAGTCAGCAT CATGGTAAAT
    GCCATTAATC AGATACCTAT GTATAAAGGT TGGATCCTGG ATGGCTTTCC AATGACATTA
    AACCAAGCAA AGCTTTTGGA GGAAGCACTC ACAGGCTACA ACAGAAACCT CATAGAACTG
    GAGGGAAAGA AATCACACAC ATCCACATTA GCTGTTGACC CTACAGCTTC CAAAGAAGTG
    CCTCTTCCCC CTTCTGCATT TGATTTTGTC ATGTTATTAG ACATTTCAGA TAATTCCTCA
    CTGAATCGCA TGAATGACAT CATGGCTGAA GCATTTTCAA GTGAAACTCC TCTTGAAGAT
    ATCAATCAGC GTGTAGCTGC TAAAAACAAA GATACAGATG AGGACCAGAA TTTAAGAGAT
    CAAATACAGC ACAGAATTGT AGGCTTCCTG GACAACTGGC CTTCATTGGA GCAGTGGTTT
    ACAGAGCCAG AAAACATTTT GATAAAAATC AATGCTGAAG TAGATAAACA GTCATTGTGC
    CAAAAAGTAA AGGAAATGTT TATGACTGAA ATAATGAAAA AAGAGAATAA AGTGAAAAAG
    AAATTAGAAG AAAAGGAAGC TGAGAGAAAA GAAGAAGTTT CCCTGACTGA GAAGACATCC
    CCTCCTCCTC CTCCTCCTCC CCCTCCGGAA CCAGAGAAAG AGAAAGAAGT CCACCTTCCA
    CAGGAGCGGT CCAAAACTCC TGCTGCGAAA GGAAAGCAAG CATCAGAACC CCCGCATGGT
    AATCGAGAAC CTCTTCAGGA AGGAAAAGGG AAGAAAAGTG AAACTTCACT CAAAAGAAAA
    GGTTCTCCTA GAGGAAAATC GCTAGGAGGG AAGATTCCAC TCAAGAAATC ACCTGCCGAG
    TCTACAGATG TTTCACCGGT CCCAGTAGTG CCAGCCCCAT CGAAGCCCGG ATCAGAAGAG
    TGGGTGTATG TAAATGAACC AATTCCTGAG GAAATACCAT CATTTTTAGT ACCATACTGG
    AAACTGATAG AAAATTCCTA TATAAATTCC ATCAAAACAG TACTCAGGCA GTTGAGAGAA
    GACCAGCATG CCGTACTTGC TTACTTATAT GATATTAGAA CAGGCTTCCA GCAGTTTCTG
    AGGCGTCCAG ACCACAAGCA AGACTTCGTG TCTCAGTGGC AGGCGGATTT CAACTCCCTT
    CCTGACGACC TGTGGGATGA TGAGGAAACA AAGGCTGAGC TGCACCAAAG AGTGAATGAT
    TTCCGGGACC GCCTGTGGGA CATTTGCGAT GCCCGCAAGG AAGAAGCAGA GCAGGAGCGT
    CTGGACATCA TTAACGAGAG CTGGTTGCAG GACTCGACGG GAATTGCCAT GAACCATTTC
    TTCTCACTGA TGCAGGCAGA ACTGAACCGT TTCCAAGATA CGAAGAGACT CCTGCAAGAT
    TATTACAGGG CCATGGAGTG TAAAATTCCA ATGGATGACA CTAAGAGATT CACTCGAGTC
    CCACTGGTCC AACTGGATAG TAAAGATATT TTGGAAAGCC AGCTTAGAAT TCCCCTGGTT
    CCTCGAAGAT CCATTTCTCC AGAGTCAGCT TTATCCAAAC CAAAAACAAA AAGCATCCTG
    AAGGGCAAGA TCGATTACAT GCTGGAAAGT GTAGAGTTAA ACTTTGAGGC TGATGAGAAG
    ATGGTGATGG ATACGTGGCA GCAGTCTTCT TTGGCGATAT CTCACATGGT GGCTGCTGAA
    ATTCACCAGA GGCTCATGGA AGAAGAAAAA GAAAACCAGC CAGCAGAAAC AAAAGAAAAA
    CTTCCTCAGG CGGCTGCAAA TAAAAAAGTG AAAAAGGAGC CTCCGCCACC ACCACCACCA
    CCCAAGAAAA AAAAAGAGGA CAAAAAAGGA AAAGGTAAAT CCCCACCTAT GACAGAAGCT
    GCTCCTGTAG TATTGCCAGC AGAAGAGACT GAGGAAGTGG AAGTGAAAAA CGAACTGAAG
    CTCAGAATAA AGGAAGAGCA TCTTGCTGCC CTGCAATTTG AAGAGATAGC TACACAGTTC
    CGACTTGAAC TGATAAAGGT AAAAGCATTG GCTTTCCTGG AAGAGTTAGT AATAAAGGCT
    GTTGATGTAT ATAGACTCAT GGAAAAGTGG CTCGGTGAGA GGTACTTGAA TGAAATGGCC
    AGTGTGGAAA AACTAACTGG GGTAGCTCGC TATCACATTG AAACATCAAC CAAAATTCAG
    AATGAACTTT ATTTAGACCA AGAAGACTTC TTCATTAATG GCGATATCAA AGTCTTTCCA
    GATCCACCTC CTCCGGTACG CCCTCCACCT GTGGAAAAGG AGGAAAACGG CACCCTGACC
    ATCGAGCAGC TGGACAACCT CCGGGATCAG TTTTTAGATA TAGCGCCCAA AGGCAGGGAT
    TCTCCAGACG ATGTGAGTGG ATTCACCAAG AAGCAGAAAA GGCCTCGGAA GCTTGACCAT
    TACCTCCCAA AGAAGAACAA TCAATTTTTG AAAATTTGGC TTAAAAAATA CCCTTGGCTT
    GTATACGATG AGATACTAAA TCTTATGTTC TGTGGCCTGT GTCGTAAACA TGGAGTGACA
    TCAAAGGGAA ATCAGGTGAG TTTTTTTTAT GGCACGGATA ACTTCAGGAC TGAATTTCTC
    AACGCGCATC ATCTGAGCGA GCCTCACGCC AAGGCGTCCC TCATGGAAGC CACGAGTGGT
    TCTCCAGGGA GCAGAGCAAC CACGGAGCTG ATGGTGAGGA CCATGAGCAA GGTGACCCTC
    GGCAGGGTGG AGAACCTGTT CCGGTCCTGC CACGCCATCG CCAAAACCGG ACGTCCCCTC
    AAAGACTTCC TCTGGATGTG TTCGCTGGAC GATATGAAGG GAGTTGATAT TGGCCCGGTG
    TTCAGAACCA GTAAATCGGC CAGAACTTTT ACGTACTTTA TTGCTGAAGT GGAACGGAGA
    GCTCTAAGAG AGAAGTTAGA AAAAAGTCAG TTCTTCTCCG TCCTCAGTGA TGGGATTGCA
    GCCAGTTCAG TTGAGGAAGC TGAACTCGTA TATGTGCAGT TTGCACGCGC AGGGAGAGTG
    CACTGCCAAA TCGTCGGGGT GCAGACCGTG GAGAAGAAGG AGCCCCGGGC GATAAAACAT
    GCCATCGAGA AAACTCTAGA GAGAAATCTT CAACTTCGGC TGTCCAACCA AGACTGGGCA
    AAGAAACTGG TGGGTTTTGG AAGTGATGGA GCTCCGGGTT CAGAGGGAGA AAACAGCAGT
    GAGGTGGCCC TGCTTTTAAG GGAAATCCAG CCAGGTGTGC TGCCTGTTTA CTGCTTTGCC
    CATCACCTCG AACTATCTTA CCAGGCGGTG TTCCGGAACG TTCCCCTCTA CAACCATGTG
    AAAGACCTTC TTCATAGCAT GTACTGCTTC TATCACGATT CTCCTGCTCA CAGGAGTGCT
    CTGCTAACTG CTTTCAAGGG CCTCCACCTT CGACCTGCAA TGCCCTCTCA AGTCAGCGGC
    CAGAGGTGGC TTCAGGGATT GCAGACTGCC CTCCAGAACT TTCTCAAGGG ATATCCAGCA
    ATTGTCCAGC AGCTGCATTC AGCAGAAGAA AGCAGAAGTG ACAGCAGTCA TCAGAAAGCC
    AAAGAACTCC TGGAGTCTCT CCTCCAGGCA GACGTCGTCA AATTTGTCCA CTTCTTGTTG
    GATGTCGTCA ATGTCCTTAG CATACTGTCC CACGTCAGTC AGAACAGAAA TTCCTCAATT
    GCTGATATAT TTGCCACCTT AGAGTCAACA CTGGAAATGC TCCAAATCTA TCAGACAAGA
    CCAGGGCCGA AAGAACGTGG GGTGGATTCA GTCACACACT TTCATGGTAA CTGCCTAGGA
    GGAAAGGGAA ACATTTCTGA TGTGAGAAAC ATGGTTTTAA CACATCTTAT CAAGAAGCTG
    CGAGGCTGTT TCAGAGATGC CAACCAAGAT GTGGTGAAGG CAACCATGAT TGGAAGCTTT
    AAACTGTGGC CGGCAAAAAT AAATCAAGAA TTTGGAGAAA AAGAGGTGGC CATCCTGATT
    GCACATTATG AACCAGTATT AGAGGCAGCC AAGGTGAAAA TTGACCAAGT AGACACAGAA
    TGGAGCATGT TGAAATTGGA GATCTACTCT AGATTTCAGA ACATTCGCAA ACTGACCTGG
    GATTTTGTGA ATTCTGTTTA TTTACACAAG TATCCAAACA TCCTGACACT CATTGACCTC
    GTGCTGAGCC TGCCTGCAAG TTCAGCTGAG GCAGGACGCG GCTTTAGTCA GATGAAGCGC
    ATCAGGTCCC AAATGCATGC CAAGGTCAAG GCTGACAGCA TGACGGATCT CCTGATCATT
    CAGTTGAATT CTCCAGACAT TAACAACTTT GACCCTAGGA AAGCCATTCA TTTATGGAAT
    ACAAGAACAC CATCCTCAAC TGAGGACACA AGACCTAACT CAGATTGCTC ATCAAACTCA
    GAAAGCCTTG GGGAAAGTGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.