Spef2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spef2
Entrez Gene ID	320277
Full Name	sperm flagellar 2
Synonyms	C230086A09Rik
General protein information	Preferred Names sperm flagellar 2 Names sperm flagellar protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_177123.4	NP_796097.2	sperm flagellar protein 2 isoform 2
NM_001305044.1	NP_001291973.1	sperm flagellar protein 2 isoform 3
XM_017316667.1	XP_017172156.1	sperm flagellar protein 2 isoform X1
XM_017316668.1	XP_017172157.1	sperm flagellar protein 2 isoform X2
NM_001305042.1	NP_001291971.1	sperm flagellar protein 2 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPEF2	XP_546353.2
Gallus gallus (chicken)	SPEF2	XP_003643024.2
Danio rerio (zebrafish)	spef2	NP_001121704.1
Homo sapiens (human)	SPEF2	NP_079143.3
	LOC703498	XP_001091802.1
Mus musculus (house mouse)	Spef2	NP_796097.2
Pan troglodytes (chimpanzee)	SPEF2	XP_001143539.2
Bos taurus (cattle)	SPEF2	XP_002696427.2
Rattus norvegicus (Norway rat)	Spef2	NP_072142.1
Xenopus tropicalis (tropical clawed frog)	spef2	XP_002940003.2

Gene Ontology
Bibliography

Related articles in PubMed

Cilia-related protein SPEF2 regulates osteoblast differentiation.
Lehti MS, Henriksson H, Rummukainen P, Wang F, Uusitalo-Kylm?l? L, Kiviranta R, Heino TJ, Kotaja N, Sironen A
Scientific reports8(1)859(2018 Jan)

SPEF2 functions in microtubule-mediated transport in elongating spermatids to ensure proper male germ cell differentiation.
Lehti MS, Zhang FP, Kotaja N, Sironen A
Development (Cambridge, England)144(14)2683-2693(2017 07)

Strain-dependent brain defects in mouse models of primary ciliary dyskinesia with mutations in Pcdp1 and Spef2.
Finn R, Evans CC, Lee L
Neuroscience277552-67(2014 Sep)

Enhanced response to pulmonary Streptococcus pneumoniae infection is associated with primary ciliary dyskinesia in mice lacking Pcdp1 and Spef2.
McKenzie CW, Klonoski JM, Maier T, Trujillo G, Vitiello PF, Huber VC, Lee L
Cilia2(1)18(2013 Dec)

Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.
Sironen A, Kotaja N, Mulhern H, Wyatt TA, Sisson JH, Pavlik JA, Miiluniemi M, Fleming MD, Lee L
Biology of reproduction85(4)690-701(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Investigated the function of SPEF2 during spermatogenesis using a male germ cell-specific Spef2 knockout model. In addition to defects in sperm tail development, we observed a duplication of the basal body and failure in manchette migration resulting in an abnormal head shape.
Title: SPEF2 functions in microtubule-mediated transport in elongating spermatids to ensure proper male germ cell differentiation.

Data indicate that SPEF2 is required for cilia function/motility; defect in SPEF2 results in primary ciliary dyskinesia. SPEF2 plays important role in spermatogenesis and sperm flagellum assembly. (base/AA sequence data submitted to GenBank)
Title: Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.

The crucial importance of SPEF2 in sperm differentiation and involvement of SPEF2 in structuring of the sperm tail.
Title: Expression of SPEF2 during mouse spermatogenesis and identification of IFT20 as an interacting protein.

The following Spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu74626	NM_001305044.1 Latest version!	Mus musculus sperm flagellar 2 (Spef2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu05955	NM_177123.4 Latest version!	Mus musculus sperm flagellar 2 (Spef2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu79135	XM_017316667.1 Latest version!	Mus musculus sperm flagellar 2 (Spef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu79136	XM_017316668.1 Latest version!	Mus musculus sperm flagellar 2 (Spef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMu74625	NM_001305042.1 Latest version!	Mus musculus sperm flagellar 2 (Spef2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spef2 ( NM_001305044.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291973.1 Mus musculus sperm flagellar 2 (Spef2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu74626
Clone ID Related Accession (Same CDS sequence)	NM_001305044.1
Accession Version	NM_001305044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	sperm flagellar protein 2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK048961.1, AK041992.1 and AC133586.12. Transcript Variant: This variant (3) lacks numerous 3' exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK041992.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA GGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TGCAGAATTT TCAGAGAGCA GGGGTTCAAC AGCCAAGCTT 
AACAATTTCT CCCGCTTGCA ACCAACACTT CACCTGCTGG GTTTGCAGTT TGATCAGAAT 
GTGGCTCAAA GCATCATCAC AGAGAAGCCT GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACA GGGCTGACTG GACTAGAGAT ACAAACCATG 
CAACCCCAGA CAAACTTGAG GCTTCAGACC CTCAAAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATC 
CCCAAGCCTG AATCCAACCG TACTTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA TTAAAGAAAG TGTATCCAAG ACTTCTTTAG AAACAACAGA CCAGACAACT 
GCAGAACTGC TCAACACTTA TTCAGATGAT GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGACG CTTTCGCACG CGAGCAAAGG GAAAAAAGAC GACGCAGATT GTTGATGGAT 
CAGCTAATGG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA TGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCCGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTTGATAGA GAAGCGGCTC TTGCCAAACA AGCCAAGATT 
GATTTTGCAG AACAAACCTT GAGGGAGAAG GAAATTCATG AAAAGATCTC TGTGGAAAGA 
GCCCAACAGC GTTATAAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATGCTA 
GATTTGTGCA CGAAGGTGGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACATAAG 
ATGATGCACG ATTGGAAAGA ACTGTTTTTT AGTGGAATTC CCATATATGA ACAGACTTCT 
CTTACACATG GACAAACAGA ACCCACTGAG GATCACCGTG CAGAAGTGAA GAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGATTAT AAGGTACATA CTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291973.1
CDS	100..1470
Translation	MSEILCQWLNQELRVSRTVSPKSFAKAFSNGYLIGEVLFKFELQSDFAEFSESRGSTAKLNNFSRLQPTLHLLGLQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNLRLQTLKSEAFREQRLNRRRQNEIMAKIQAAIIQIPKPESNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQIKESVSKTSLETTDQTTAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLMAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALAKQAKIDFAEQTLREKEIHEKISVERAQQRYKKHYGICAEILDQMLDLCTKVADYRLLTNNLIPHKMMHDWKELFFSGIPIYEQTSLTHGQTEPTEDHRAEVKKRNLLDSKDYEDYKVHTNM

Target ORF information:

RefSeq Version	NM_001305044.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm flagellar 2 (Spef2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305044.1

ORF Insert Sequence:

ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA GGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TGCAGAATTT TCAGAGAGCA GGGGTTCAAC AGCCAAGCTT 
AACAATTTCT CCCGCTTGCA ACCAACACTT CACCTGCTGG GTTTGCAGTT TGATCAGAAT 
GTGGCTCAAA GCATCATCAC AGAGAAGCCT GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACA GGGCTGACTG GACTAGAGAT ACAAACCATG 
CAACCCCAGA CAAACTTGAG GCTTCAGACC CTCAAAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATC 
CCCAAGCCTG AATCCAACCG TACTTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA TTAAAGAAAG TGTATCCAAG ACTTCTTTAG AAACAACAGA CCAGACAACT 
GCAGAACTGC TCAACACTTA TTCAGATGAT GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGACG CTTTCGCACG CGAGCAAAGG GAAAAAAGAC GACGCAGATT GTTGATGGAT 
CAGCTAATGG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA TGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCCGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTTGATAGA GAAGCGGCTC TTGCCAAACA AGCCAAGATT 
GATTTTGCAG AACAAACCTT GAGGGAGAAG GAAATTCATG AAAAGATCTC TGTGGAAAGA 
GCCCAACAGC GTTATAAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATGCTA 
GATTTGTGCA CGAAGGTGGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACATAAG 
ATGATGCACG ATTGGAAAGA ACTGTTTTTT AGTGGAATTC CCATATATGA ACAGACTTCT 
CTTACACATG GACAAACAGA ACCCACTGAG GATCACCGTG CAGAAGTGAA GAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGATTAT AAGGTACATA CTAATATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( NM_177123.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796097.2 Mus musculus sperm flagellar 2 (Spef2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05955
Clone ID Related Accession (Same CDS sequence)	NM_177123.4
Accession Version	NM_177123.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	sperm flagellar protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK048961.1 and AC133959.4. On Feb 19, 2015 this sequence version replaced NM_177123.3. Transcript Variant: This variant (2) lacks numerous 3' exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK048961.1, BC132098.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA GGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TGCAGAATTT TCAGAGAGCA GGGGTTCAAC AGCCAAGCTT 
AACAATTTCT CCCGCTTGCA ACCAACACTT CACCTGCTGG GTTTGCAGTT TGATCAGAAT 
GTGGCTCAAA GCATCATCAC AGAGAAGCCT GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACA GGGCTGACTG GACTAGAGAT ACAAACCATG 
CAACCCCAGA CAAACTTGAG GCTTCAGACC CTCAAAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATC 
CCCAAGCCTG AATCCAACCG TACTTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA TTAAAGAAAG TGTATCCAAG ACTTCTTTAG AAACAACAGA CCAGACAACT 
GCAGAACTGC TCAACACTTA TTCAGATGAT GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGACG CTTTCGCACG CGAGCAAAGG GAAAAAAGAC GACGCAGATT GTTGATGGAT 
CAGCTAATGG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA TGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCCGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTTGATAGA GAAGCGGCTC TTGCCAAACA AGCCAAGATT 
GATTTTGCAG AACAAACCTT GAGGGAGAAG GAAATTCATG AAAAGATCTC TGTGGAAAGA 
GCCCAACAGC GTTATAAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATGCTA 
GATTTGTGCA CGAAGGTGGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACATAAG 
ATGATGCACG ATTGGAAAGA ACTGTTTTTT AGTGGAATTC CCATATATGA ACAGACTTCT 
CTTACACATG GACAAACAGA ACCCACTGAG GATCACCGTG CAGAAGTGAA GAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGATTAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAGGACATGG TTAATAGTTC ACCACCCTCC AACAATAGCA TTTTGGGTCA CGTGCTTCTG 
AGATTAATTG AAAAAGCTGA TCCATCTGCA AGTAATGCTG AAGCCACTGA ATTACCTTCA 
CTTGCGGTCA AGGGTTGTAT ACTGGGGAAG ACACTCAGTG GAAAAACTAC TGTTCTAAAG 
TCTCTACAAA ATGACTTTCC TGTTCATGTG CTTTCTATTG ATACTCTTGT CCAAGAGGCT 
ATCCAGGCGT TTCATGAAAG ACAAAAAAGC GGTAAGACAC CACCAACTCA GGAAGATGAT 
AAAAGAGATC CTGTAGTGAA CCAGGAAAAA GTGAGCAAAA CTCAGGATAA AAATGTGTTA 
GCAGTTAGTC CAGTTCCAGG GGACAGAACA TCCCAAAAAG AAGGTGTCAA GATTAATGAA 
TTTGAACAGT TCAGATCAAG TGATAGCTTT TTATCACTTA GCATGCGAGC TCAACTTGGT 
GCAAAGTCAG AGTTGATGCT TAGAAGAGGA AAGAGCATTC CAGACATACT TCTGGTCAGC 
ATCCTGGTAA ATGCTATAAA GGAGATACCT GTGGACCAAA GCTGGGTTCT TGATGGATTT 
CCAATAACAT TAAATCAAGC CAAACTCCTG GAGGAAGCAC TTACAGGGTA CAAAAGAAAA 
TTCCTACAGT TAAAGAAGAA GAAAGAACAA ATGCCCACAT TGGCCCTAGA CCCCTCAACT 
TCCACAGAGG TCTCTCTTCT CCCATCTGCA CTTGATTTTG TCATATTGCT AGATATTTCC 
GATAACTCAT CGTTGGCTCG AACTAATGAT ATCATAGCTA AAGAAATCTC ACATGAAATT 
TCTCATGAAA ATGTTGGCCG TCCTGGAACT GGTACAAGCC AAGATAATAA GAGCGAGGAC 
CGGAACTTAA GAGACCACAT ACAGCACAGA ATTGTTGGCT TCTTGGACAA CTGGCCACTG 
TTGGAAGAGT GGTTTACACA GCCAAAAAAC ATTCTGACGA AAGTCAATGC TGAAATAGAT 
GAAGCGCTCT TATGCCAAAA AGTAAAAGAA ATTTTTGCTA CTGAAACAGT AAACAAAAAG 
ATCAAAGTTG AAAAGACACT GGAAGAAAAG GAAACTGAGA AAAAGGCAGG AGCTCCCCCG 
GCTGAGCCTC CCGCTATGAG CCCTCCCCTT TCTTCTGAAG CAGAAAAAGA CAAGGAGCTT 
CATCAGGCAA AAACTCCTGG GAAAGGAAAG ACACAATCAG GTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796097.2
CDS	100..2727
Translation	MSEILCQWLNQELRVSRTVSPKSFAKAFSNGYLIGEVLFKFELQSDFAEFSESRGSTAKLNNFSRLQPTLHLLGLQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNLRLQTLKSEAFREQRLNRRRQNEIMAKIQAAIIQIPKPESNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQIKESVSKTSLETTDQTTAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLMAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALAKQAKIDFAEQTLREKEIHEKISVERAQQRYKKHYGICAEILDQMLDLCTKVADYRLLTNNLIPHKMMHDWKELFFSGIPIYEQTSLTHGQTEPTEDHRAEVKKRNLLDSKDYEDYKNMVGEWALPEDMVNSSPPSNNSILGHVLLRLIEKADPSASNAEATELPSLAVKGCILGKTLSGKTTVLKSLQNDFPVHVLSIDTLVQEAIQAFHERQKSGKTPPTQEDDKRDPVVNQEKVSKTQDKNVLAVSPVPGDRTSQKEGVKINEFEQFRSSDSFLSLSMRAQLGAKSELMLRRGKSIPDILLVSILVNAIKEIPVDQSWVLDGFPITLNQAKLLEEALTGYKRKFLQLKKKKEQMPTLALDPSTSTEVSLLPSALDFVILLDISDNSSLARTNDIIAKEISHEISHENVGRPGTGTSQDNKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTQPKNILTKVNAEIDEALLCQKVKEIFATETVNKKIKVEKTLEEKETEKKAGAPPAEPPAMSPPLSSEAEKDKELHQAKTPGKGKTQSGD

Target ORF information:

RefSeq Version	NM_177123.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm flagellar 2 (Spef2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177123.4

ORF Insert Sequence:

ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA GGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TGCAGAATTT TCAGAGAGCA GGGGTTCAAC AGCCAAGCTT 
AACAATTTCT CCCGCTTGCA ACCAACACTT CACCTGCTGG GTTTGCAGTT TGATCAGAAT 
GTGGCTCAAA GCATCATCAC AGAGAAGCCT GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACA GGGCTGACTG GACTAGAGAT ACAAACCATG 
CAACCCCAGA CAAACTTGAG GCTTCAGACC CTCAAAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAACAGAA GACGACAAAA TGAGATAATG GCCAAAATCC AGGCAGCCAT TATACAGATC 
CCCAAGCCTG AATCCAACCG TACTTTGAAA GCTATTGAGG CTCAAAAACT GATGAAAAAG 
AAAAAGGAGG CAGAGGACGT GGCCAATGAG ATTAAGAAAT TTGAAGCACT AATTAAAAAG 
GATCTCCAAA TTAAAGAAAG TGTATCCAAG ACTTCTTTAG AAACAACAGA CCAGACAACT 
GCAGAACTGC TCAACACTTA TTCAGATGAT GACTACATTA AAAAGATCCA GAAACGCCTA 
GAAGAAGACG CTTTCGCACG CGAGCAAAGG GAAAAAAGAC GACGCAGATT GTTGATGGAT 
CAGCTAATGG CCCATGAAGC ACAGGAGGAG GCCTATCGGG AAGAGCAGCT CATTCACCGG 
CTCATGCGCC AGTCCCAGCA GGAGCGCAGG ATTGCTGTGC AGCTCATGCA TGTTCGCCAC 
GAGAAGGAAG TTTTGTGGCA AAATAGAATT TTCCGAGAGA AACAATTTGA AGAAAGACGA 
CTTAAGGATT TCCAGGATGC TCTTGATAGA GAAGCGGCTC TTGCCAAACA AGCCAAGATT 
GATTTTGCAG AACAAACCTT GAGGGAGAAG GAAATTCATG AAAAGATCTC TGTGGAAAGA 
GCCCAACAGC GTTATAAGAA GCATTATGGG ATATGTGCAG AGATTTTGGA TCAAATGCTA 
GATTTGTGCA CGAAGGTGGC AGACTATAGG TTGCTGACAA ATAATCTTAT TCCACATAAG 
ATGATGCACG ATTGGAAAGA ACTGTTTTTT AGTGGAATTC CCATATATGA ACAGACTTCT 
CTTACACATG GACAAACAGA ACCCACTGAG GATCACCGTG CAGAAGTGAA GAAAAGGAAC 
TTACTCGATA GCAAAGATTA TGAAGATTAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAGGACATGG TTAATAGTTC ACCACCCTCC AACAATAGCA TTTTGGGTCA CGTGCTTCTG 
AGATTAATTG AAAAAGCTGA TCCATCTGCA AGTAATGCTG AAGCCACTGA ATTACCTTCA 
CTTGCGGTCA AGGGTTGTAT ACTGGGGAAG ACACTCAGTG GAAAAACTAC TGTTCTAAAG 
TCTCTACAAA ATGACTTTCC TGTTCATGTG CTTTCTATTG ATACTCTTGT CCAAGAGGCT 
ATCCAGGCGT TTCATGAAAG ACAAAAAAGC GGTAAGACAC CACCAACTCA GGAAGATGAT 
AAAAGAGATC CTGTAGTGAA CCAGGAAAAA GTGAGCAAAA CTCAGGATAA AAATGTGTTA 
GCAGTTAGTC CAGTTCCAGG GGACAGAACA TCCCAAAAAG AAGGTGTCAA GATTAATGAA 
TTTGAACAGT TCAGATCAAG TGATAGCTTT TTATCACTTA GCATGCGAGC TCAACTTGGT 
GCAAAGTCAG AGTTGATGCT TAGAAGAGGA AAGAGCATTC CAGACATACT TCTGGTCAGC 
ATCCTGGTAA ATGCTATAAA GGAGATACCT GTGGACCAAA GCTGGGTTCT TGATGGATTT 
CCAATAACAT TAAATCAAGC CAAACTCCTG GAGGAAGCAC TTACAGGGTA CAAAAGAAAA 
TTCCTACAGT TAAAGAAGAA GAAAGAACAA ATGCCCACAT TGGCCCTAGA CCCCTCAACT 
TCCACAGAGG TCTCTCTTCT CCCATCTGCA CTTGATTTTG TCATATTGCT AGATATTTCC 
GATAACTCAT CGTTGGCTCG AACTAATGAT ATCATAGCTA AAGAAATCTC ACATGAAATT 
TCTCATGAAA ATGTTGGCCG TCCTGGAACT GGTACAAGCC AAGATAATAA GAGCGAGGAC 
CGGAACTTAA GAGACCACAT ACAGCACAGA ATTGTTGGCT TCTTGGACAA CTGGCCACTG 
TTGGAAGAGT GGTTTACACA GCCAAAAAAC ATTCTGACGA AAGTCAATGC TGAAATAGAT 
GAAGCGCTCT TATGCCAAAA AGTAAAAGAA ATTTTTGCTA CTGAAACAGT AAACAAAAAG 
ATCAAAGTTG AAAAGACACT GGAAGAAAAG GAAACTGAGA AAAAGGCAGG AGCTCCCCCG 
GCTGAGCCTC CCGCTATGAG CCCTCCCCTT TCTTCTGAAG CAGAAAAAGA CAAGGAGCTT 
CATCAGGCAA AAACTCCTGG GAAAGGAAAG ACACAATCAG GTGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( XM_017316667.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172156.1 Mus musculus sperm flagellar 2 (Spef2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79135
Clone ID Related Accession (Same CDS sequence)	XM_017316667.1
Accession Version	XM_017316667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCTC AACTTGGTGC AAAGTCAGAG TTGATGCTTA GAAGAGGAAA GAGCATTCCA 
GACATACTTC TGGTCAGCAT CCTGGTAAAT GCTATAAAGG AGATACCTGT GGACCAAAGC 
TGGGTTCTTG ATGGATTTCC AATAACATTA AATCAAGCCA AACTCCTGGA GGAAGCACTT 
ACAGGGTACA AAAGAAAATT CCTACAGTTA AAGAAGAAGA AAGAACAAAT GCCCACATTG 
GCCCTAGACC CCTCAACTTC CACAGAGGTC TCTCTTCTCC CATCTGCACT TGATTTTGTC 
ATATTGCTAG ATATTTCCGA TAACTCATCG TTGGCTCGAA CTAATGATAT CATAGCTAAA 
GAAATCTCAC ATGAAATTTC TCATGAAAAT GTTGGCCGTC CTGGAACTGG TACAAGCCAA 
GATAATAAGA GCGAGGACCG GAACTTAAGA GACCACATAC AGCACAGAAT TGTTGGCTTC 
TTGGACAACT GGCCACTGTT GGAAGAGTGG TTTACACAGC CAAAAAACAT TCTGACGAAA 
GTCAATGCTG AAATAGATGA AGCGCTCTTA TGCCAAAAAG TAAAAGAAAT TTTTGCTACT 
GAAACAGTAA ACAAAAAGAT CAAAGTTGAA AAGACACTGG AAGAAAAGGA AACTGAGAAA 
AAGGCAGGAG CTCCCCCGGC TGAGCCTCCC GCTATGAGCC CTCCCCTTTC TTCTGAAGCA 
GAAAAAGACA AGGAGCTTCA TCAGGCAAAA ACTCCTGGGA AAGGAAAGAC ACAATCAGAA 
ACCCAGCATG GTAAGCAAGA ATCTCAACCT GAAGGAAAAG GAAAGAAAGT TTCTCCTAAA 
GGAAAAGCAC AAGGAGGCAA AGTTTCAGTA AAGAAATCAC CGGTTGGCTC AGCAGAAGTA 
TCACCCACAC CAACAGCACC ACCGCCACCT AAGGCAGGAA CAGAAGAATG GGTTTATGTG 
AATGAGCCTA TACCGGAGGA ACTCCCTTCA TTTTTGGTAC CTTACTGGGA ACTGATAGAG 
AAATCCTACA TCAACCACAT CAAAACAGTA CTTCGACATC TCAGAGAGAG ACAGCACAAT 
GTGCTCTCTT ATTTATATGA GACTAGGACA AGTTTCCAGG AGTTTCTACG GCGTCCAGAT 
CACAAGCAAG ATTTTGTATC TCAGTGGCAG GCTGATTTCA ACTCTGTTCC TGAAGACCTG 
TGGGAAGATG AGGAAACAAA GGCTGAGCTA CACCAAAGAG TAAATGATCT GCGAGACCGC 
CTATGGGACA TCTGTGAAGC GAGGAAGGAA GAGGCGGAAC AGGAGAGGCT TGATATCATT 
AATGAGAGCT GGCTGCAGGA CTCTATCGGA ATCACAATGA ATCATTTCTT TTCCCTGATG 
CAGGCAGAAG TGAACCGTTT TCAAGATACC AAAAGACTCC TTCAAGATTA TTACAGAGCA 
ATGGAATCCA AAATCCCTCT GGAAGACAGT AAGAAATTTA CTCGGGTCCC ATTGGTTCAG 
CTTGATGGTA AAGAAATTTC AGAAAGCCAG CTCAGAATTC CTCTAGTTCC TCGAATATCC 
AATTCTCCAG AAAATTCTGC CGTCAAGCCA AAGGTGGGGA CATTTCTGAA GGGCAGATCT 
GATCCTCCTT TAGAAGTCCT GGAAGCCAAC TTTGAAATAG ATGAGAAGAT TTTATTGGAC 
ACCTGGCAGC AGGCTTCATT AGCCATCTCT AACATGGTGG CTGCTGAAGT TCACCAAAGG 
CTCACAGAGG AAGAAAAAGA ACCACCCCAA TTAGATTCCA AAGAAAAGTC TCCTCAGTCA 
GGTGCAAATA AAAAAGCCAA GAAGGAGAAA GAAGCACCCA AGAAGAAAAA GACAGACAAG 
AAAGGAAAAG GCAAATCATC CCCCGTGGCA GAAGTCAGTC CAGTCACAGT CACACCTGAG 
GAAATGGCTG AGATGGAAAA GAGGAATGAA CTGAGGCTCA GGATAAAGGA AGAACATCTT 
GCTGCCCTGC AGACAGAAGA ACAAGCCGCA CAATTTCGAC TTGAGCTAAT AAAGTTGAAA 
GCTTTGTCCG TCCTGGAAGA TTTAGTAACA AAGGTGATTG ATGTCTACAG GCTCATGGAA 
AAATGGCTTG GTAAGAGATA TTTGAATGAA ATGGCCAGTA TACAGAAATT GACCGAATTA 
GCTCGCTACC ACATTGAAAC AGCTACAAAA ATTCAAAATG AGATTTACTT AAGCCAAGAA 
GACTTCTATA TCAATGGAGA CATAAAAGTC TTCCCAGATC CTTCTCCACC AACCCGCCCA 
CCACCAGTAG AAAGGGAAGA AAATGGTACC CTAACAATCG AACAGCTTGA TAATCTTCGA 
GATCAGTTCT TAGACATGGC ACCTAAAGGC AAAGACTCTC CAAATGCTGT GATTCCAGTC 
ACCAAAAAGC AGAAAAAGTC ACAAAAAGTT GACTCCTTCC TACCAGAAAG AAATCACCAG 
TTCTTGAAAA TTTGGCTAAA AAAATACCCT TGGCTTGTAT ATGATGAGCA ACTAAACCTT 
ATGTTCTGTG TCCTGTGTCG TAAGCACGGA GTAACGTCAG GGGGAAGTAC AGTAAGTTTC 
CTCTATGGCA CGGACAACTT CAGGGCTGAA TTTCTCAGTG CGCATCACCT CAGTGAAGCC 
CATGCCAAGG CGTCACTGAT GGAAGTGACC AGTGGTTCTC CAGTGAACAG AGCTGCCACT 
GAGCTGACTG TAAGGACTAT GAGCAAAGTG ACCCTGGCGA GAGTGGAGAA CTTATTTAGA 
TCATGCCACG CCCTAGTCAA GACCGGACAT CCCCTCAAAG ATTTTATCTG GATGTGTAAA 
TTGGATGATA TGAAGGGTGT TGATATTGGC CCAGTCTTCA GAACTAAAAA ATCAGCAAGA 
ATATTTACAT ATTTCATTGC TGAAGTGGAG AGGAAAAATC TAAGAGAAAA CCTAGAAAAG 
AGTAACTTTT TCTCTGTCAT CACTGATGGA ATTGTAGACA GTCTAGGCAA GGAAGCCAAA 
ATGGTCTATG TTCAATTTGC ACATGCAGGA AAAGTGCAAT GTCAGATTGT TGGGTTGCAG 
CCAGTAGAAC CAAAGGACTC TTTGGCAATA AAAAATGCTA TTGAGAAAAC TCTAGAGACA 
AATCTTCATC TTAAGCTGTC AAGCCAAGAC TGGGCGAAGA AGTTGGTTGG TTTTGGAAGT 
GATGATTCCC CTGGAATAGA GGGGAAGAAC AGAGTGGCCC TGCTTTTGAG AGAAATACAG 
CCATGTGTGC AAAGTATGTA TTGCTTCGCA CATCATCTAG AACTGTCCTA CAAATCGATG 
TTCCAGAGTG TCCCTCTCTA CAATGACCTT GAAGCACTCC TCACCAGTAT TTACCATTTC 
TATCACAACT CTCCTCTCTT CAAGAGTTCT CTGATAACTG CCTTCAGGGG CCTCCACCTC 
CAACCTGTGA TGCCTTCTCA AATAGGAGGG AGACGGTGGC TTCGTGGACT ACAAGCATCC 
CTCCAGAACT TTCTCAAGGG ATACCCAGCA ATTGTTCAGC AACTGCATTC AGTAGGTAAA 
GGCCAAAATG ATACCAGTGA ACAGAATGCC AACAGTCTTC TAGATCTCGT TCTCCAAGCG 
GATATCATCA GATTTGCTCA TTTCTTGACA GACATCATTA GTATCCTTAG CCTTCTGTCC 
CACACCAGTA GGAACAGAAA TTCTTCAATT GCAGATGTAT TTGCTTCCTT GGAGTCAACC 
CTGGAAATGC TCCAGATATA TCAATCAAGA CCAGGGCCAA AAGAACGCAG GGTGGATTCA 
GCCACACACT TTCACGGTAA CTGCCTCAGG GGGGAAGCAA ACATTTCTTC CGTGAGAAAG 
GTGGTTTTAA CACATCTTAT CGAGAGATTG CGAGGCTGTT TTAGAGATGC CAGCCTTGAT 
GTGGTGAGAG CAACTGTGAT TGGAAGCTTT AGACTGTGGC CTACAAAGAT AAATCAAGAA 
TTTGGAGTCA AAGAGATATC CATCCTGATT TCATATTATG AACCAGTTCT CAAAGCTGCC 
AATGTGAAAA CGGATGAGGT AGATACCGAG TGGAGCATGT TAAAATTAGA ATTATATGGC 
AGATTTCAGA ACATTCGAAA GCTGACCTGG GATTTTGTAA ACTCCATTTA TTCCCATAAA 
TATCCAAGTG TCCTGATGCT TGTTGACTTG GTGCTGGCCC TCCCTGCAAG CTCAGCAGAG 
CCAGAACGTG GGTGTAGTCA GAGGAAACGC ATGGATGAGA GAATCAAGCC TGAAACCATG 
ACAGACATCC TAACCGTTCA GCTAAACTCT CCAGACATTA GAAGTTTTGA CCCTAGGAAA 
GCTATCCATT TATGGAACAT GCAGACGCAA TTGCCGATGG CTCACAGGGG ATCTAATCTT 
GACAGTTCAT CAGACTCAGA AAGCCAAGAT GAAAGTGATG AGCAGTGCGA CTTGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172156.1
CDS	249..4688
Translation	MRAQLGAKSELMLRRGKSIPDILLVSILVNAIKEIPVDQSWVLDGFPITLNQAKLLEEALTGYKRKFLQLKKKKEQMPTLALDPSTSTEVSLLPSALDFVILLDISDNSSLARTNDIIAKEISHEISHENVGRPGTGTSQDNKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTQPKNILTKVNAEIDEALLCQKVKEIFATETVNKKIKVEKTLEEKETEKKAGAPPAEPPAMSPPLSSEAEKDKELHQAKTPGKGKTQSETQHGKQESQPEGKGKKVSPKGKAQGGKVSVKKSPVGSAEVSPTPTAPPPPKAGTEEWVYVNEPIPEELPSFLVPYWELIEKSYINHIKTVLRHLRERQHNVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDSIGITMNHFFSLMQAEVNRFQDTKRLLQDYYRAMESKIPLEDSKKFTRVPLVQLDGKEISESQLRIPLVPRISNSPENSAVKPKVGTFLKGRSDPPLEVLEANFEIDEKILLDTWQQASLAISNMVAAEVHQRLTEEEKEPPQLDSKEKSPQSGANKKAKKEKEAPKKKKTDKKGKGKSSPVAEVSPVTVTPEEMAEMEKRNELRLRIKEEHLAALQTEEQAAQFRLELIKLKALSVLEDLVTKVIDVYRLMEKWLGKRYLNEMASIQKLTELARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPSPPTRPPPVEREENGTLTIEQLDNLRDQFLDMAPKGKDSPNAVIPVTKKQKKSQKVDSFLPERNHQFLKIWLKKYPWLVYDEQLNLMFCVLCRKHGVTSGGSTVSFLYGTDNFRAEFLSAHHLSEAHAKASLMEVTSGSPVNRAATELTVRTMSKVTLARVENLFRSCHALVKTGHPLKDFIWMCKLDDMKGVDIGPVFRTKKSARIFTYFIAEVERKNLRENLEKSNFFSVITDGIVDSLGKEAKMVYVQFAHAGKVQCQIVGLQPVEPKDSLAIKNAIEKTLETNLHLKLSSQDWAKKLVGFGSDDSPGIEGKNRVALLLREIQPCVQSMYCFAHHLELSYKSMFQSVPLYNDLEALLTSIYHFYHNSPLFKSSLITAFRGLHLQPVMPSQIGGRRWLRGLQASLQNFLKGYPAIVQQLHSVGKGQNDTSEQNANSLLDLVLQADIIRFAHFLTDIISILSLLSHTSRNRNSSIADVFASLESTLEMLQIYQSRPGPKERRVDSATHFHGNCLRGEANISSVRKVVLTHLIERLRGCFRDASLDVVRATVIGSFRLWPTKINQEFGVKEISILISYYEPVLKAANVKTDEVDTEWSMLKLELYGRFQNIRKLTWDFVNSIYSHKYPSVLMLVDLVLALPASSAEPERGCSQRKRMDERIKPETMTDILTVQLNSPDIRSFDPRKAIHLWNMQTQLPMAHRGSNLDSSSDSESQDESDEQCDLC

Target ORF information:

RefSeq Version	XM_017316667.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm flagellar 2 (Spef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316667.1

ORF Insert Sequence:

ATGCGAGCTC AACTTGGTGC AAAGTCAGAG TTGATGCTTA GAAGAGGAAA GAGCATTCCA 
GACATACTTC TGGTCAGCAT CCTGGTAAAT GCTATAAAGG AGATACCTGT GGACCAAAGC 
TGGGTTCTTG ATGGATTTCC AATAACATTA AATCAAGCCA AACTCCTGGA GGAAGCACTT 
ACAGGGTACA AAAGAAAATT CCTACAGTTA AAGAAGAAGA AAGAACAAAT GCCCACATTG 
GCCCTAGACC CCTCAACTTC CACAGAGGTC TCTCTTCTCC CATCTGCACT TGATTTTGTC 
ATATTGCTAG ATATTTCCGA TAACTCATCG TTGGCTCGAA CTAATGATAT CATAGCTAAA 
GAAATCTCAC ATGAAATTTC TCATGAAAAT GTTGGCCGTC CTGGAACTGG TACAAGCCAA 
GATAATAAGA GCGAGGACCG GAACTTAAGA GACCACATAC AGCACAGAAT TGTTGGCTTC 
TTGGACAACT GGCCACTGTT GGAAGAGTGG TTTACACAGC CAAAAAACAT TCTGACGAAA 
GTCAATGCTG AAATAGATGA AGCGCTCTTA TGCCAAAAAG TAAAAGAAAT TTTTGCTACT 
GAAACAGTAA ACAAAAAGAT CAAAGTTGAA AAGACACTGG AAGAAAAGGA AACTGAGAAA 
AAGGCAGGAG CTCCCCCGGC TGAGCCTCCC GCTATGAGCC CTCCCCTTTC TTCTGAAGCA 
GAAAAAGACA AGGAGCTTCA TCAGGCAAAA ACTCCTGGGA AAGGAAAGAC ACAATCAGAA 
ACCCAGCATG GTAAGCAAGA ATCTCAACCT GAAGGAAAAG GAAAGAAAGT TTCTCCTAAA 
GGAAAAGCAC AAGGAGGCAA AGTTTCAGTA AAGAAATCAC CGGTTGGCTC AGCAGAAGTA 
TCACCCACAC CAACAGCACC ACCGCCACCT AAGGCAGGAA CAGAAGAATG GGTTTATGTG 
AATGAGCCTA TACCGGAGGA ACTCCCTTCA TTTTTGGTAC CTTACTGGGA ACTGATAGAG 
AAATCCTACA TCAACCACAT CAAAACAGTA CTTCGACATC TCAGAGAGAG ACAGCACAAT 
GTGCTCTCTT ATTTATATGA GACTAGGACA AGTTTCCAGG AGTTTCTACG GCGTCCAGAT 
CACAAGCAAG ATTTTGTATC TCAGTGGCAG GCTGATTTCA ACTCTGTTCC TGAAGACCTG 
TGGGAAGATG AGGAAACAAA GGCTGAGCTA CACCAAAGAG TAAATGATCT GCGAGACCGC 
CTATGGGACA TCTGTGAAGC GAGGAAGGAA GAGGCGGAAC AGGAGAGGCT TGATATCATT 
AATGAGAGCT GGCTGCAGGA CTCTATCGGA ATCACAATGA ATCATTTCTT TTCCCTGATG 
CAGGCAGAAG TGAACCGTTT TCAAGATACC AAAAGACTCC TTCAAGATTA TTACAGAGCA 
ATGGAATCCA AAATCCCTCT GGAAGACAGT AAGAAATTTA CTCGGGTCCC ATTGGTTCAG 
CTTGATGGTA AAGAAATTTC AGAAAGCCAG CTCAGAATTC CTCTAGTTCC TCGAATATCC 
AATTCTCCAG AAAATTCTGC CGTCAAGCCA AAGGTGGGGA CATTTCTGAA GGGCAGATCT 
GATCCTCCTT TAGAAGTCCT GGAAGCCAAC TTTGAAATAG ATGAGAAGAT TTTATTGGAC 
ACCTGGCAGC AGGCTTCATT AGCCATCTCT AACATGGTGG CTGCTGAAGT TCACCAAAGG 
CTCACAGAGG AAGAAAAAGA ACCACCCCAA TTAGATTCCA AAGAAAAGTC TCCTCAGTCA 
GGTGCAAATA AAAAAGCCAA GAAGGAGAAA GAAGCACCCA AGAAGAAAAA GACAGACAAG 
AAAGGAAAAG GCAAATCATC CCCCGTGGCA GAAGTCAGTC CAGTCACAGT CACACCTGAG 
GAAATGGCTG AGATGGAAAA GAGGAATGAA CTGAGGCTCA GGATAAAGGA AGAACATCTT 
GCTGCCCTGC AGACAGAAGA ACAAGCCGCA CAATTTCGAC TTGAGCTAAT AAAGTTGAAA 
GCTTTGTCCG TCCTGGAAGA TTTAGTAACA AAGGTGATTG ATGTCTACAG GCTCATGGAA 
AAATGGCTTG GTAAGAGATA TTTGAATGAA ATGGCCAGTA TACAGAAATT GACCGAATTA 
GCTCGCTACC ACATTGAAAC AGCTACAAAA ATTCAAAATG AGATTTACTT AAGCCAAGAA 
GACTTCTATA TCAATGGAGA CATAAAAGTC TTCCCAGATC CTTCTCCACC AACCCGCCCA 
CCACCAGTAG AAAGGGAAGA AAATGGTACC CTAACAATCG AACAGCTTGA TAATCTTCGA 
GATCAGTTCT TAGACATGGC ACCTAAAGGC AAAGACTCTC CAAATGCTGT GATTCCAGTC 
ACCAAAAAGC AGAAAAAGTC ACAAAAAGTT GACTCCTTCC TACCAGAAAG AAATCACCAG 
TTCTTGAAAA TTTGGCTAAA AAAATACCCT TGGCTTGTAT ATGATGAGCA ACTAAACCTT 
ATGTTCTGTG TCCTGTGTCG TAAGCACGGA GTAACGTCAG GGGGAAGTAC AGTAAGTTTC 
CTCTATGGCA CGGACAACTT CAGGGCTGAA TTTCTCAGTG CGCATCACCT CAGTGAAGCC 
CATGCCAAGG CGTCACTGAT GGAAGTGACC AGTGGTTCTC CAGTGAACAG AGCTGCCACT 
GAGCTGACTG TAAGGACTAT GAGCAAAGTG ACCCTGGCGA GAGTGGAGAA CTTATTTAGA 
TCATGCCACG CCCTAGTCAA GACCGGACAT CCCCTCAAAG ATTTTATCTG GATGTGTAAA 
TTGGATGATA TGAAGGGTGT TGATATTGGC CCAGTCTTCA GAACTAAAAA ATCAGCAAGA 
ATATTTACAT ATTTCATTGC TGAAGTGGAG AGGAAAAATC TAAGAGAAAA CCTAGAAAAG 
AGTAACTTTT TCTCTGTCAT CACTGATGGA ATTGTAGACA GTCTAGGCAA GGAAGCCAAA 
ATGGTCTATG TTCAATTTGC ACATGCAGGA AAAGTGCAAT GTCAGATTGT TGGGTTGCAG 
CCAGTAGAAC CAAAGGACTC TTTGGCAATA AAAAATGCTA TTGAGAAAAC TCTAGAGACA 
AATCTTCATC TTAAGCTGTC AAGCCAAGAC TGGGCGAAGA AGTTGGTTGG TTTTGGAAGT 
GATGATTCCC CTGGAATAGA GGGGAAGAAC AGAGTGGCCC TGCTTTTGAG AGAAATACAG 
CCATGTGTGC AAAGTATGTA TTGCTTCGCA CATCATCTAG AACTGTCCTA CAAATCGATG 
TTCCAGAGTG TCCCTCTCTA CAATGACCTT GAAGCACTCC TCACCAGTAT TTACCATTTC 
TATCACAACT CTCCTCTCTT CAAGAGTTCT CTGATAACTG CCTTCAGGGG CCTCCACCTC 
CAACCTGTGA TGCCTTCTCA AATAGGAGGG AGACGGTGGC TTCGTGGACT ACAAGCATCC 
CTCCAGAACT TTCTCAAGGG ATACCCAGCA ATTGTTCAGC AACTGCATTC AGTAGGTAAA 
GGCCAAAATG ATACCAGTGA ACAGAATGCC AACAGTCTTC TAGATCTCGT TCTCCAAGCG 
GATATCATCA GATTTGCTCA TTTCTTGACA GACATCATTA GTATCCTTAG CCTTCTGTCC 
CACACCAGTA GGAACAGAAA TTCTTCAATT GCAGATGTAT TTGCTTCCTT GGAGTCAACC 
CTGGAAATGC TCCAGATATA TCAATCAAGA CCAGGGCCAA AAGAACGCAG GGTGGATTCA 
GCCACACACT TTCACGGTAA CTGCCTCAGG GGGGAAGCAA ACATTTCTTC CGTGAGAAAG 
GTGGTTTTAA CACATCTTAT CGAGAGATTG CGAGGCTGTT TTAGAGATGC CAGCCTTGAT 
GTGGTGAGAG CAACTGTGAT TGGAAGCTTT AGACTGTGGC CTACAAAGAT AAATCAAGAA 
TTTGGAGTCA AAGAGATATC CATCCTGATT TCATATTATG AACCAGTTCT CAAAGCTGCC 
AATGTGAAAA CGGATGAGGT AGATACCGAG TGGAGCATGT TAAAATTAGA ATTATATGGC 
AGATTTCAGA ACATTCGAAA GCTGACCTGG GATTTTGTAA ACTCCATTTA TTCCCATAAA 
TATCCAAGTG TCCTGATGCT TGTTGACTTG GTGCTGGCCC TCCCTGCAAG CTCAGCAGAG 
CCAGAACGTG GGTGTAGTCA GAGGAAACGC ATGGATGAGA GAATCAAGCC TGAAACCATG 
ACAGACATCC TAACCGTTCA GCTAAACTCT CCAGACATTA GAAGTTTTGA CCCTAGGAAA 
GCTATCCATT TATGGAACAT GCAGACGCAA TTGCCGATGG CTCACAGGGG ATCTAATCTT 
GACAGTTCAT CAGACTCAGA AAGCCAAGAT GAAAGTGATG AGCAGTGCGA CTTGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( XM_017316668.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172157.1 Mus musculus sperm flagellar 2 (Spef2), transcript variant X2, mRNA.

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CloneID	OMu79136
Clone ID Related Accession (Same CDS sequence)	XM_017316668.1
Accession Version	XM_017316668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGCTC AACTTGGTGC AAAGTCAGAG TTGATGCTTA GAAGAGGAAA GAGCATTCCA 
GACATACTTC TGGTCAGCAT CCTGGTAAAT GCTATAAAGG AGATACCTGT GGACCAAAGC 
TGGGTTCTTG ATGGATTTCC AATAACATTA AATCAAGCCA AACTCCTGGA GGAAGCACTT 
ACAGGGTACA AAAGAAAATT CCTACAGTTA AAGAAGAAGA AAGAACAAAT GCCCACATTG 
GCCCTAGACC CCTCAACTTC CACAGAGGTC TCTCTTCTCC CATCTGCACT TGATTTTGTC 
ATATTGCTAG ATATTTCCGA TAACTCATCG TTGGCTCGAA CTAATGATAT CATAGCTAAA 
GAAATCTCAC ATGAAATTTC TCATGAAAAT GTTGGCCGTC CTGGAACTGG TACAAGCCAA 
GATAATAAGA GCGAGGACCG GAACTTAAGA GACCACATAC AGCACAGAAT TGTTGGCTTC 
TTGGACAACT GGCCACTGTT GGAAGAGTGG TTTACACAGC CAAAAAACAT TCTGACGAAA 
GTCAATGCTG AAATAGATGA AGCGCTCTTA TGCCAAAAAG TAAAAGAAAT TTTTGCTACT 
GAAACAGTAA ACAAAAAGAT CAAAGTTGAA AAGACACTGG AAGAAAAGGA AACTGAGAAA 
AAGGCAGGAG CTCCCCCGGC TGAGCCTCCC GCTATGAGCC CTCCCCTTTC TTCTGAAGCA 
GAAAAAGACA AGGAGCTTCA TCAGGCAAAA ACTCCTGGGA AAGGAAAGAC ACAATCAGTT 
TCTCCTAAAG GAAAAGCACA AGGAGGCAAA GTTTCAGTAA AGAAATCACC GGTTGGCTCA 
GCAGAAGTAT CACCCACACC AACAGCACCA CCGCCACCTA AGGCAGGAAC AGAAGAATGG 
GTTTATGTGA ATGAGCCTAT ACCGGAGGAA CTCCCTTCAT TTTTGGTACC TTACTGGGAA 
CTGATAGAGA AATCCTACAT CAACCACATC AAAACAGTAC TTCGACATCT CAGAGAGAGA 
CAGCACAATG TGCTCTCTTA TTTATATGAG ACTAGGACAA GTTTCCAGGA GTTTCTACGG 
CGTCCAGATC ACAAGCAAGA TTTTGTATCT CAGTGGCAGG CTGATTTCAA CTCTGTTCCT 
GAAGACCTGT GGGAAGATGA GGAAACAAAG GCTGAGCTAC ACCAAAGAGT AAATGATCTG 
CGAGACCGCC TATGGGACAT CTGTGAAGCG AGGAAGGAAG AGGCGGAACA GGAGAGGCTT 
GATATCATTA ATGAGAGCTG GCTGCAGGAC TCTATCGGAA TCACAATGAA TCATTTCTTT 
TCCCTGATGC AGGCAGAAGT GAACCGTTTT CAAGATACCA AAAGACTCCT TCAAGATTAT 
TACAGAGCAA TGGAATCCAA AATCCCTCTG GAAGACAGTA AGAAATTTAC TCGGGTCCCA 
TTGGTTCAGC TTGATGGTAA AGAAATTTCA GAAAGCCAGC TCAGAATTCC TCTAGTTCCT 
CGAATATCCA ATTCTCCAGA AAATTCTGCC GTCAAGCCAA AGGTGGGGAC ATTTCTGAAG 
GGCAGATCTG ATCCTCCTTT AGAAGTCCTG GAAGCCAACT TTGAAATAGA TGAGAAGATT 
TTATTGGACA CCTGGCAGCA GGCTTCATTA GCCATCTCTA ACATGGTGGC TGCTGAAGTT 
CACCAAAGGC TCACAGAGGA AGAAAAAGAA CCACCCCAAT TAGATTCCAA AGAAAAGTCT 
CCTCAGTCAG GTGCAAATAA AAAAGCCAAG AAGGAGAAAG AAGCACCCAA GAAGAAAAAG 
ACAGACAAGA AAGGAAAAGG CAAATCATCC CCCGTGGCAG AAGTCAGTCC AGTCACAGTC 
ACACCTGAGG AAATGGCTGA GATGGAAAAG AGGAATGAAC TGAGGCTCAG GATAAAGGAA 
GAACATCTTG CTGCCCTGCA GACAGAAGAA CAAGCCGCAC AATTTCGACT TGAGCTAATA 
AAGTTGAAAG CTTTGTCCGT CCTGGAAGAT TTAGTAACAA AGGTGATTGA TGTCTACAGG 
CTCATGGAAA AATGGCTTGG TAAGAGATAT TTGAATGAAA TGGCCAGTAT ACAGAAATTG 
ACCGAATTAG CTCGCTACCA CATTGAAACA GCTACAAAAA TTCAAAATGA GATTTACTTA 
AGCCAAGAAG ACTTCTATAT CAATGGAGAC ATAAAAGTCT TCCCAGATCC TTCTCCACCA 
ACCCGCCCAC CACCAGTAGA AAGGGAAGAA AATGGTACCC TAACAATCGA ACAGCTTGAT 
AATCTTCGAG ATCAGTTCTT AGACATGGCA CCTAAAGGCA AAGACTCTCC AAATGCTGTG 
ATTCCAGTCA CCAAAAAGCA GAAAAAGTCA CAAAAAGTTG ACTCCTTCCT ACCAGAAAGA 
AATCACCAGT TCTTGAAAAT TTGGCTAAAA AAATACCCTT GGCTTGTATA TGATGAGCAA 
CTAAACCTTA TGTTCTGTGT CCTGTGTCGT AAGCACGGAG TAACGTCAGG GGGAAGTACA 
GTAAGTTTCC TCTATGGCAC GGACAACTTC AGGGCTGAAT TTCTCAGTGC GCATCACCTC 
AGTGAAGCCC ATGCCAAGGC GTCACTGATG GAAGTGACCA GTGGTTCTCC AGTGAACAGA 
GCTGCCACTG AGCTGACTGT AAGGACTATG AGCAAAGTGA CCCTGGCGAG AGTGGAGAAC 
TTATTTAGAT CATGCCACGC CCTAGTCAAG ACCGGACATC CCCTCAAAGA TTTTATCTGG 
ATGTGTAAAT TGGATGATAT GAAGGGTGTT GATATTGGCC CAGTCTTCAG AACTAAAAAA 
TCAGCAAGAA TATTTACATA TTTCATTGCT GAAGTGGAGA GGAAAAATCT AAGAGAAAAC 
CTAGAAAAGA GTAACTTTTT CTCTGTCATC ACTGATGGAA TTGTAGACAG TCTAGGCAAG 
GAAGCCAAAA TGGTCTATGT TCAATTTGCA CATGCAGGAA AAGTGCAATG TCAGATTGTT 
GGGTTGCAGC CAGTAGAACC AAAGGACTCT TTGGCAATAA AAAATGCTAT TGAGAAAACT 
CTAGAGACAA ATCTTCATCT TAAGCTGTCA AGCCAAGACT GGGCGAAGAA GTTGGTTGGT 
TTTGGAAGTG ATGATTCCCC TGGAATAGAG GGGAAGAACA GAGTGGCCCT GCTTTTGAGA 
GAAATACAGC CATGTGTGCA AAGTATGTAT TGCTTCGCAC ATCATCTAGA ACTGTCCTAC 
AAATCGATGT TCCAGAGTGT CCCTCTCTAC AATGACCTTG AAGCACTCCT CACCAGTATT 
TACCATTTCT ATCACAACTC TCCTCTCTTC AAGAGTTCTC TGATAACTGC CTTCAGGGGC 
CTCCACCTCC AACCTGTGAT GCCTTCTCAA ATAGGAGGGA GACGGTGGCT TCGTGGACTA 
CAAGCATCCC TCCAGAACTT TCTCAAGGGA TACCCAGCAA TTGTTCAGCA ACTGCATTCA 
GTAGGTAAAG GCCAAAATGA TACCAGTGAA CAGAATGCCA ACAGTCTTCT AGATCTCGTT 
CTCCAAGCGG ATATCATCAG ATTTGCTCAT TTCTTGACAG ACATCATTAG TATCCTTAGC 
CTTCTGTCCC ACACCAGTAG GAACAGAAAT TCTTCAATTG CAGATGTATT TGCTTCCTTG 
GAGTCAACCC TGGAAATGCT CCAGATATAT CAATCAAGAC CAGGGCCAAA AGAACGCAGG 
GTGGATTCAG CCACACACTT TCACGGTAAC TGCCTCAGGG GGGAAGCAAA CATTTCTTCC 
GTGAGAAAGG TGGTTTTAAC ACATCTTATC GAGAGATTGC GAGGCTGTTT TAGAGATGCC 
AGCCTTGATG TGGTGAGAGC AACTGTGATT GGAAGCTTTA GACTGTGGCC TACAAAGATA 
AATCAAGAAT TTGGAGTCAA AGAGATATCC ATCCTGATTT CATATTATGA ACCAGTTCTC 
AAAGCTGCCA ATGTGAAAAC GGATGAGGTA GATACCGAGT GGAGCATGTT AAAATTAGAA 
TTATATGGCA GATTTCAGAA CATTCGAAAG CTGACCTGGG ATTTTGTAAA CTCCATTTAT 
TCCCATAAAT ATCCAAGTGT CCTGATGCTT GTTGACTTGG TGCTGGCCCT CCCTGCAAGC 
TCAGCAGAGC CAGAACGTGG GTGTAGTCAG AGGAAACGCA TGGATGAGAG AATCAAGCCT 
GAAACCATGA CAGACATCCT AACCGTTCAG CTAAACTCTC CAGACATTAG AAGTTTTGAC 
CCTAGGAAAG CTATCCATTT ATGGAACATG CAGACGCAAT TGCCGATGGC TCACAGGGGA 
TCTAATCTTG ACAGTTCATC AGACTCAGAA AGCCAAGATG AAAGTGATGA GCAGTGCGAC 
TTGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172157.1
CDS	249..4637
Translation	MRAQLGAKSELMLRRGKSIPDILLVSILVNAIKEIPVDQSWVLDGFPITLNQAKLLEEALTGYKRKFLQLKKKKEQMPTLALDPSTSTEVSLLPSALDFVILLDISDNSSLARTNDIIAKEISHEISHENVGRPGTGTSQDNKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTQPKNILTKVNAEIDEALLCQKVKEIFATETVNKKIKVEKTLEEKETEKKAGAPPAEPPAMSPPLSSEAEKDKELHQAKTPGKGKTQSVSPKGKAQGGKVSVKKSPVGSAEVSPTPTAPPPPKAGTEEWVYVNEPIPEELPSFLVPYWELIEKSYINHIKTVLRHLRERQHNVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDSIGITMNHFFSLMQAEVNRFQDTKRLLQDYYRAMESKIPLEDSKKFTRVPLVQLDGKEISESQLRIPLVPRISNSPENSAVKPKVGTFLKGRSDPPLEVLEANFEIDEKILLDTWQQASLAISNMVAAEVHQRLTEEEKEPPQLDSKEKSPQSGANKKAKKEKEAPKKKKTDKKGKGKSSPVAEVSPVTVTPEEMAEMEKRNELRLRIKEEHLAALQTEEQAAQFRLELIKLKALSVLEDLVTKVIDVYRLMEKWLGKRYLNEMASIQKLTELARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPSPPTRPPPVEREENGTLTIEQLDNLRDQFLDMAPKGKDSPNAVIPVTKKQKKSQKVDSFLPERNHQFLKIWLKKYPWLVYDEQLNLMFCVLCRKHGVTSGGSTVSFLYGTDNFRAEFLSAHHLSEAHAKASLMEVTSGSPVNRAATELTVRTMSKVTLARVENLFRSCHALVKTGHPLKDFIWMCKLDDMKGVDIGPVFRTKKSARIFTYFIAEVERKNLRENLEKSNFFSVITDGIVDSLGKEAKMVYVQFAHAGKVQCQIVGLQPVEPKDSLAIKNAIEKTLETNLHLKLSSQDWAKKLVGFGSDDSPGIEGKNRVALLLREIQPCVQSMYCFAHHLELSYKSMFQSVPLYNDLEALLTSIYHFYHNSPLFKSSLITAFRGLHLQPVMPSQIGGRRWLRGLQASLQNFLKGYPAIVQQLHSVGKGQNDTSEQNANSLLDLVLQADIIRFAHFLTDIISILSLLSHTSRNRNSSIADVFASLESTLEMLQIYQSRPGPKERRVDSATHFHGNCLRGEANISSVRKVVLTHLIERLRGCFRDASLDVVRATVIGSFRLWPTKINQEFGVKEISILISYYEPVLKAANVKTDEVDTEWSMLKLELYGRFQNIRKLTWDFVNSIYSHKYPSVLMLVDLVLALPASSAEPERGCSQRKRMDERIKPETMTDILTVQLNSPDIRSFDPRKAIHLWNMQTQLPMAHRGSNLDSSSDSESQDESDEQCDLC

Target ORF information:

RefSeq Version	XM_017316668.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm flagellar 2 (Spef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316668.1

ORF Insert Sequence:

ATGCGAGCTC AACTTGGTGC AAAGTCAGAG TTGATGCTTA GAAGAGGAAA GAGCATTCCA 
GACATACTTC TGGTCAGCAT CCTGGTAAAT GCTATAAAGG AGATACCTGT GGACCAAAGC 
TGGGTTCTTG ATGGATTTCC AATAACATTA AATCAAGCCA AACTCCTGGA GGAAGCACTT 
ACAGGGTACA AAAGAAAATT CCTACAGTTA AAGAAGAAGA AAGAACAAAT GCCCACATTG 
GCCCTAGACC CCTCAACTTC CACAGAGGTC TCTCTTCTCC CATCTGCACT TGATTTTGTC 
ATATTGCTAG ATATTTCCGA TAACTCATCG TTGGCTCGAA CTAATGATAT CATAGCTAAA 
GAAATCTCAC ATGAAATTTC TCATGAAAAT GTTGGCCGTC CTGGAACTGG TACAAGCCAA 
GATAATAAGA GCGAGGACCG GAACTTAAGA GACCACATAC AGCACAGAAT TGTTGGCTTC 
TTGGACAACT GGCCACTGTT GGAAGAGTGG TTTACACAGC CAAAAAACAT TCTGACGAAA 
GTCAATGCTG AAATAGATGA AGCGCTCTTA TGCCAAAAAG TAAAAGAAAT TTTTGCTACT 
GAAACAGTAA ACAAAAAGAT CAAAGTTGAA AAGACACTGG AAGAAAAGGA AACTGAGAAA 
AAGGCAGGAG CTCCCCCGGC TGAGCCTCCC GCTATGAGCC CTCCCCTTTC TTCTGAAGCA 
GAAAAAGACA AGGAGCTTCA TCAGGCAAAA ACTCCTGGGA AAGGAAAGAC ACAATCAGTT 
TCTCCTAAAG GAAAAGCACA AGGAGGCAAA GTTTCAGTAA AGAAATCACC GGTTGGCTCA 
GCAGAAGTAT CACCCACACC AACAGCACCA CCGCCACCTA AGGCAGGAAC AGAAGAATGG 
GTTTATGTGA ATGAGCCTAT ACCGGAGGAA CTCCCTTCAT TTTTGGTACC TTACTGGGAA 
CTGATAGAGA AATCCTACAT CAACCACATC AAAACAGTAC TTCGACATCT CAGAGAGAGA 
CAGCACAATG TGCTCTCTTA TTTATATGAG ACTAGGACAA GTTTCCAGGA GTTTCTACGG 
CGTCCAGATC ACAAGCAAGA TTTTGTATCT CAGTGGCAGG CTGATTTCAA CTCTGTTCCT 
GAAGACCTGT GGGAAGATGA GGAAACAAAG GCTGAGCTAC ACCAAAGAGT AAATGATCTG 
CGAGACCGCC TATGGGACAT CTGTGAAGCG AGGAAGGAAG AGGCGGAACA GGAGAGGCTT 
GATATCATTA ATGAGAGCTG GCTGCAGGAC TCTATCGGAA TCACAATGAA TCATTTCTTT 
TCCCTGATGC AGGCAGAAGT GAACCGTTTT CAAGATACCA AAAGACTCCT TCAAGATTAT 
TACAGAGCAA TGGAATCCAA AATCCCTCTG GAAGACAGTA AGAAATTTAC TCGGGTCCCA 
TTGGTTCAGC TTGATGGTAA AGAAATTTCA GAAAGCCAGC TCAGAATTCC TCTAGTTCCT 
CGAATATCCA ATTCTCCAGA AAATTCTGCC GTCAAGCCAA AGGTGGGGAC ATTTCTGAAG 
GGCAGATCTG ATCCTCCTTT AGAAGTCCTG GAAGCCAACT TTGAAATAGA TGAGAAGATT 
TTATTGGACA CCTGGCAGCA GGCTTCATTA GCCATCTCTA ACATGGTGGC TGCTGAAGTT 
CACCAAAGGC TCACAGAGGA AGAAAAAGAA CCACCCCAAT TAGATTCCAA AGAAAAGTCT 
CCTCAGTCAG GTGCAAATAA AAAAGCCAAG AAGGAGAAAG AAGCACCCAA GAAGAAAAAG 
ACAGACAAGA AAGGAAAAGG CAAATCATCC CCCGTGGCAG AAGTCAGTCC AGTCACAGTC 
ACACCTGAGG AAATGGCTGA GATGGAAAAG AGGAATGAAC TGAGGCTCAG GATAAAGGAA 
GAACATCTTG CTGCCCTGCA GACAGAAGAA CAAGCCGCAC AATTTCGACT TGAGCTAATA 
AAGTTGAAAG CTTTGTCCGT CCTGGAAGAT TTAGTAACAA AGGTGATTGA TGTCTACAGG 
CTCATGGAAA AATGGCTTGG TAAGAGATAT TTGAATGAAA TGGCCAGTAT ACAGAAATTG 
ACCGAATTAG CTCGCTACCA CATTGAAACA GCTACAAAAA TTCAAAATGA GATTTACTTA 
AGCCAAGAAG ACTTCTATAT CAATGGAGAC ATAAAAGTCT TCCCAGATCC TTCTCCACCA 
ACCCGCCCAC CACCAGTAGA AAGGGAAGAA AATGGTACCC TAACAATCGA ACAGCTTGAT 
AATCTTCGAG ATCAGTTCTT AGACATGGCA CCTAAAGGCA AAGACTCTCC AAATGCTGTG 
ATTCCAGTCA CCAAAAAGCA GAAAAAGTCA CAAAAAGTTG ACTCCTTCCT ACCAGAAAGA 
AATCACCAGT TCTTGAAAAT TTGGCTAAAA AAATACCCTT GGCTTGTATA TGATGAGCAA 
CTAAACCTTA TGTTCTGTGT CCTGTGTCGT AAGCACGGAG TAACGTCAGG GGGAAGTACA 
GTAAGTTTCC TCTATGGCAC GGACAACTTC AGGGCTGAAT TTCTCAGTGC GCATCACCTC 
AGTGAAGCCC ATGCCAAGGC GTCACTGATG GAAGTGACCA GTGGTTCTCC AGTGAACAGA 
GCTGCCACTG AGCTGACTGT AAGGACTATG AGCAAAGTGA CCCTGGCGAG AGTGGAGAAC 
TTATTTAGAT CATGCCACGC CCTAGTCAAG ACCGGACATC CCCTCAAAGA TTTTATCTGG 
ATGTGTAAAT TGGATGATAT GAAGGGTGTT GATATTGGCC CAGTCTTCAG AACTAAAAAA 
TCAGCAAGAA TATTTACATA TTTCATTGCT GAAGTGGAGA GGAAAAATCT AAGAGAAAAC 
CTAGAAAAGA GTAACTTTTT CTCTGTCATC ACTGATGGAA TTGTAGACAG TCTAGGCAAG 
GAAGCCAAAA TGGTCTATGT TCAATTTGCA CATGCAGGAA AAGTGCAATG TCAGATTGTT 
GGGTTGCAGC CAGTAGAACC AAAGGACTCT TTGGCAATAA AAAATGCTAT TGAGAAAACT 
CTAGAGACAA ATCTTCATCT TAAGCTGTCA AGCCAAGACT GGGCGAAGAA GTTGGTTGGT 
TTTGGAAGTG ATGATTCCCC TGGAATAGAG GGGAAGAACA GAGTGGCCCT GCTTTTGAGA 
GAAATACAGC CATGTGTGCA AAGTATGTAT TGCTTCGCAC ATCATCTAGA ACTGTCCTAC 
AAATCGATGT TCCAGAGTGT CCCTCTCTAC AATGACCTTG AAGCACTCCT CACCAGTATT 
TACCATTTCT ATCACAACTC TCCTCTCTTC AAGAGTTCTC TGATAACTGC CTTCAGGGGC 
CTCCACCTCC AACCTGTGAT GCCTTCTCAA ATAGGAGGGA GACGGTGGCT TCGTGGACTA 
CAAGCATCCC TCCAGAACTT TCTCAAGGGA TACCCAGCAA TTGTTCAGCA ACTGCATTCA 
GTAGGTAAAG GCCAAAATGA TACCAGTGAA CAGAATGCCA ACAGTCTTCT AGATCTCGTT 
CTCCAAGCGG ATATCATCAG ATTTGCTCAT TTCTTGACAG ACATCATTAG TATCCTTAGC 
CTTCTGTCCC ACACCAGTAG GAACAGAAAT TCTTCAATTG CAGATGTATT TGCTTCCTTG 
GAGTCAACCC TGGAAATGCT CCAGATATAT CAATCAAGAC CAGGGCCAAA AGAACGCAGG 
GTGGATTCAG CCACACACTT TCACGGTAAC TGCCTCAGGG GGGAAGCAAA CATTTCTTCC 
GTGAGAAAGG TGGTTTTAAC ACATCTTATC GAGAGATTGC GAGGCTGTTT TAGAGATGCC 
AGCCTTGATG TGGTGAGAGC AACTGTGATT GGAAGCTTTA GACTGTGGCC TACAAAGATA 
AATCAAGAAT TTGGAGTCAA AGAGATATCC ATCCTGATTT CATATTATGA ACCAGTTCTC 
AAAGCTGCCA ATGTGAAAAC GGATGAGGTA GATACCGAGT GGAGCATGTT AAAATTAGAA 
TTATATGGCA GATTTCAGAA CATTCGAAAG CTGACCTGGG ATTTTGTAAA CTCCATTTAT 
TCCCATAAAT ATCCAAGTGT CCTGATGCTT GTTGACTTGG TGCTGGCCCT CCCTGCAAGC 
TCAGCAGAGC CAGAACGTGG GTGTAGTCAG AGGAAACGCA TGGATGAGAG AATCAAGCCT 
GAAACCATGA CAGACATCCT AACCGTTCAG CTAAACTCTC CAGACATTAG AAGTTTTGAC 
CCTAGGAAAG CTATCCATTT ATGGAACATG CAGACGCAAT TGCCGATGGC TCACAGGGGA 
TCTAATCTTG ACAGTTCATC AGACTCAGAA AGCCAAGATG AAAGTGATGA GCAGTGCGAC 
TTGTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spef2 ( NM_001305042.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001291971.1 Mus musculus sperm flagellar 2 (Spef2), transcript variant 1, mRNA.

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CloneID	OMu74625
Clone ID Related Accession (Same CDS sequence)	NM_001305042.1
Accession Version	NM_001305042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	sperm flagellar protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK048961.1, HQ856050.1, AC133959.4 and AV253706.2. On Feb 19, 2015 this sequence version replaced XM_006520087.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: HQ856050.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA GGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TGCAGAATTT TCAGAGAGCA GGGGTTCAAC AGCCAAGCTT 
AACAATTTCT CCCGCTTGCA ACCAACACTT CACCTGCTGG GTTTGCAGTT TGATCAGAAT 
GTGGCTCAAA GCATCATCAC AGAGAAGCCT GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACA GGGCTGACTG GACTAGAGAT ACAAACCATG 
CAACCCCAGA CAAACTTGAG GCTTCAGACC CTCAAAAGTG AGGCTTTTCG AGAGAGACTT 
AAAAACCTGA TACCACGGCA AACCGATTTC AATCTGATGC GGGTTACATG CAGGTTTCAA 
GAAAAGTGTA AACAAATGAA AGAAGATCTT GCCCGAATGA ATTTTGAGAA GTTTGAAAAA 
ATTCAAAAAC TTGAGGAAGA GCAAAGACAC TTTAATATCG AAAAGCAACG CTTAAACAGA 
AGACGACAAA ATGAGATAAT GGCCAAAATC CAGGCAGCCA TTATACAGAT CCCCAAGCCT 
GAATCCAACC GTACTTTGAA AGCTATTGAG GCTCAAAAAC TGATGAAAAA GAAAAAGGAG 
GCAGAGGACG TGGCCAATGA GATTAAGAAA TTTGAAGCAC TAATTAAAAA GGATCTCCAA 
ATTAAAGAAA GTGTATCCAA GACTTCTTTA GAAACAACAG ACCAGACAAC TGCAGAACTG 
CTCAACACTT ATTCAGATGA TGACTACATT AAAAAGATCC AGAAACGCCT AGAAGAAGAC 
GCTTTCGCAC GCGAGCAAAG GGAAAAAAGA CGACGCAGAT TGTTGATGGA TCAGCTAATG 
GCCCATGAAG CACAGGAGGA GGCCTATCGG GAAGAGCAGC TCATTCACCG GCTCATGCGC 
CAGTCCCAGC AGGAGCGCAG GATTGCTGTG CAGCTCATGC ATGTTCGCCA CGAGAAGGAA 
GTTTTGTGGC AAAATAGAAT TTTCCGAGAG AAACAATTTG AAGAAAGACG ACTTAAGGAT 
TTCCAGGATG CTCTTGATAG AGAAGCGGCT CTTGCCAAAC AAGCCAAGAT TGATTTTGCA 
GAACAAACCT TGAGGGAGAA GGAAATTCAT GAAAAGATCT CTGTGGAAAG AGCCCAACAG 
CGTTATAAGA AGCATTATGG GATATGTGCA GAGATTTTGG ATCAAATGCT AGATTTGTGC 
ACGAAGGTGG CAGACTATAG GTTGCTGACA AATAATCTTA TTCCACATAA GATGATGCAC 
GATTGGAAAG AACTGTTTTT TAGTGGAATT CCCATATATG AACAGACTTC TCTTACACAT 
GGACAAACAG AACCCACTGA GGATCACCGT GCAGAAGTGA AGAAAAGGAA CTTACTCGAT 
AGCAAAGATT ATGAAGATTA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAGGACATG 
GTTAATAGTT CACCACCCTC CAACAATAGC ATTTTGGGTC ACGTGCTTCT GAGATTAATT 
GAAAAAGCTG ATCCATCTGC AAGTAATGCT GAAGCCACTG AATTACCTTC ACTTGCGGTC 
AAGGGTTGTA TACTGGGGAA GACACTCAGT GGAAAAACTA CTGTTCTAAA GTCTCTACAA 
AATGACTTTC CTGTTCATGT GCTTTCTATT GATACTCTTG TCCAAGAGGC TATCCAGGCG 
TTTCATGAAA GACAAAAAAG CGGTAAGACA CCACCAACTC AGGAAGATGA TAAAAGAGAT 
CCTGTAGTGA ACCAGGAAAA AGTGAGCAAA ACTCAGGATA AAAATGTGTT AGCAGTTAGT 
CCAGTTCCAG GGGACAGAAC ATCCCAAAAA GAAGGTGTCA AGATTAATGA ATTTGAACAG 
TTCAGATCAA GTGATAGCTT TTTATCACTT AGCATGCGAG CTCAACTTGG TGCAAAGTCA 
GAGTTGATGC TTAGAAGAGG AAAGAGCATT CCAGACATAC TTCTGGTCAG CATCCTGGTA 
AATGCTATAA AGGAGATACC TGTGGACCAA AGCTGGGTTC TTGATGGATT TCCAATAACA 
TTAAATCAAG CCAAACTCCT GGAGGAAGCA CTTACAGGGT ACAAAAGAAA ATTCCTACAG 
TTAAAGAAGA AGAAAGAACA AATGCCCACA TTGGCCCTAG ACCCCTCAAC TTCCACAGAG 
GTCTCTCTTC TCCCATCTGC ACTTGATTTT GTCATATTGC TAGATATTTC CGATAACTCA 
TCGTTGGCTC GAACTAATGA TATCATAGCT AAAGAAATCT CACATGAAAT TTCTCATGAA 
AATGTTGGCC GTCCTGGAAC TGGTACAAGC CAAGATAATA AGAGCGAGGA CCGGAACTTA 
AGAGACCACA TACAGCACAG AATTGTTGGC TTCTTGGACA ACTGGCCACT GTTGGAAGAG 
TGGTTTACAC AGCCAAAAAA CATTCTGACG AAAGTCAATG CTGAAATAGA TGAAGCGCTC 
TTATGCCAAA AAGTAAAAGA AATTTTTGCT ACTGAAACAG TAAACAAAAA GATCAAAGTT 
GAAAAGACAC TGGAAGAAAA GGAAACTGAG AAAAAGGCAG GAGCTCCCCC GGCTGAGCCT 
CCCGCTATGA GCCCTCCCCT TTCTTCTGAA GCAGAAAAAG ACAAGGAGCT TCATCAGGCA 
AAAACTCCTG GGAAAGGAAA GACACAATCA GAAACCCAGC ATGGTAAGCA AGAATCTCAA 
CCTGAAGGAA AAGGAAAGAA AGTTTCTCCT AAAGGAAAAG CACAAGGAGG CAAAGTTTCA 
GTAAAGAAAT CACCGGTTGG CTCAGCAGAA GTATCACCCA CACCAACAGC ACCACCGCCA 
CCTAAGGCAG GAACAGAAGA ATGGGTTTAT GTGAATGAGC CTATACCGGA GGAACTCCCT 
TCATTTTTGG TACCTTACTG GGAACTGATA GAGAAATCCT ACATCAACCA CATCAAAACA 
GTACTTCGAC ATCTCAGAGA GAGACAGCAC AATGTGCTCT CTTATTTATA TGAGACTAGG 
ACAAGTTTCC AGGAGTTTCT ACGGCGTCCA GATCACAAGC AAGATTTTGT ATCTCAGTGG 
CAGGCTGATT TCAACTCTGT TCCTGAAGAC CTGTGGGAAG ATGAGGAAAC AAAGGCTGAG 
CTACACCAAA GAGTAAATGA TCTGCGAGAC CGCCTATGGG ACATCTGTGA AGCGAGGAAG 
GAAGAGGCGG AACAGGAGAG GCTTGATATC ATTAATGAGA GCTGGCTGCA GGACTCTATC 
GGAATCACAA TGAATCATTT CTTTTCCCTG ATGCAGGCAG AAGTGAACCG TTTTCAAGAT 
ACCAAAAGAC TCCTTCAAGA TTATTACAGA GCAATGGAAT CCAAAATCCC TCTGGAAGAC 
AGTAAGAAAT TTACTCGGGT CCCATTGGTT CAGCTTGATG GTAAAGAAAT TTCAGAAAGC 
CAGCTCAGAA TTCCTCTAGT TCCTCGAATA TCCAATTCTC CAGAAAATTC TGCCGTCAAG 
CCAAAGGTGG GGACATTTCT GAAGGGCAGA TCTGATCCTC CTTTAGAAGT CCTGGAAGCC 
AACTTTGAAA TAGATGAGAA GATTTTATTG GACACCTGGC AGCAGGCTTC ATTAGCCATC 
TCTAACATGG TGGCTGCTGA AGTTCACCAA AGGCTCACAG AGGAAGAAAA AGAACCACCC 
CAATTAGATT CCAAAGAAAA GTCTCCTCAG TCAGGTGCAA ATAAAAAAGC CAAGAAGGAG 
AAAGAAGCAC CCAAGAAGAA AAAGACAGAC AAGAAAGGAA AAGGCAAATC ATCCCCCGTG 
GCAGAAGTCA GTCCAGTCAC AGTCACACCT GAGGAAATGG CTGAGATGGA AAAGAGGAAT 
GAACTGAGGC TCAGGATAAA GGAAGAACAT CTTGCTGCCC TGCAGACAGA AGAACAAGCC 
GCACAATTTC GACTTGAGCT AATAAAGTTG AAAGCTTTGT CCGTCCTGGA AGATTTAGTA 
ACAAAGGTGA TTGATGTCTA CAGGCTCATG GAAAAATGGC TTGGTAAGAG ATATTTGAAT 
GAAATGGCCA GTATACAGAA ATTGACCGAA TTAGCTCGCT ACCACATTGA AACAGCTACA 
AAAATTCAAA ATGAGATTTA CTTAAGCCAA GAAGACTTCT ATATCAATGG AGACATAAAA 
GTCTTCCCAG ATCCTTCTCC ACCAACCCGC CCACCACCAG TAGAAAGGGA AGAAAATGGT 
ACCCTAACAA TCGAACAGCT TGATAATCTT CGAGATCAGT TCTTAGACAT GGCACCTAAA 
GGCATAATCG GAAATAAAGC CTTCAGTGAC ATTCTGCTTG ATTTGATTAC CCTAAACCTT 
GGAACCAATA ACTTCCCTAG CAGCTGGATG CACCTCAGCC AACTGGACTT GCAGGAGATC 
ACATCTTTAT TAACAGTCAA CACAGAGTTC GTGGACTGGA GGAAGTTCTT GATGGTCACA 
GCCATGCCGT GGCCCATGGC CCTGGAGGAT GAGCTTCTGG ACACCCTGCA GAGGTTTAAG 
GCCCTGGATG AAGCACAGAC AGGCACCATC ACCTACGAAC AGTATAAGCA GGCTGGTCTA 
TGGTTTTCAG GAGATGAAGA TATAAAAATT CCAGAAAATC CCCTTGAACC CTTGCCATTC 
AATCGACAGG AGCATCTTAT AGAGTTCTTC TTTAGGCTGT TTGCGGACTG TGAGAAGGAG 
CCACCTCAGC TTGACTACAC TCAGATGCTG CTGTATTTTG CGTGCCACCC AGACACTTTG 
GAAGGAGTCT ACAGGGCCCT CAGTGTGGCT GTGGGAACTC ACATCTTCCG CCAGGTTGAA 
ACTCCGATGC TGATGGCAGA AAAGACCTCC ATCTCCACTG TGAGTCCCAT TGAGGAATTC 
CCTGAGACTG AGGAAAGTAG TGCAAAAGAG GACCGGGAAC TGAAGGAGGA GAAAGATGAC 
CAAAAGGAAG AAGAAATTCC CGAAAATGCA AACACTGAAA AGATTTCCAT GGAAACACTG 
CTCAAAGTGT TTGGAGGAGG AAATGAAGTA CTGGATGCCA ACAGATTTGC CAGCTACCTA 
AAATCAGAGA ATATTTATGC AGAGAATTTC ATCAAGACTT TTCAGGACCT GGGTGCCAGG 
AACCTGGAGC CAATTGCAGT TAATATTCTC TTGAAGCATC CTTACATTCA GGACCTGATT 
GCAAATTATG TAGACTATAA ATTTCCTGAC ATTAAAATGA TTCTTCAAAG GAGTGAGCAT 
GCACAAGGCA GTGATGGAGA AAGGTCACCC TCAAGACTTA CAGACGAAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291971.1
CDS	100..5496
Translation	MSEILCQWLNQELRVSRTVSPKSFAKAFSNGYLIGEVLFKFELQSDFAEFSESRGSTAKLNNFSRLQPTLHLLGLQFDQNVAQSIITEKPGAATKLLYQLYIALQKKKKTGLTGLEIQTMQPQTNLRLQTLKSEAFRERLKNLIPRQTDFNLMRVTCRFQEKCKQMKEDLARMNFEKFEKIQKLEEEQRHFNIEKQRLNRRRQNEIMAKIQAAIIQIPKPESNRTLKAIEAQKLMKKKKEAEDVANEIKKFEALIKKDLQIKESVSKTSLETTDQTTAELLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLMAHEAQEEAYREEQLIHRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQFEERRLKDFQDALDREAALAKQAKIDFAEQTLREKEIHEKISVERAQQRYKKHYGICAEILDQMLDLCTKVADYRLLTNNLIPHKMMHDWKELFFSGIPIYEQTSLTHGQTEPTEDHRAEVKKRNLLDSKDYEDYKNMVGEWALPEDMVNSSPPSNNSILGHVLLRLIEKADPSASNAEATELPSLAVKGCILGKTLSGKTTVLKSLQNDFPVHVLSIDTLVQEAIQAFHERQKSGKTPPTQEDDKRDPVVNQEKVSKTQDKNVLAVSPVPGDRTSQKEGVKINEFEQFRSSDSFLSLSMRAQLGAKSELMLRRGKSIPDILLVSILVNAIKEIPVDQSWVLDGFPITLNQAKLLEEALTGYKRKFLQLKKKKEQMPTLALDPSTSTEVSLLPSALDFVILLDISDNSSLARTNDIIAKEISHEISHENVGRPGTGTSQDNKSEDRNLRDHIQHRIVGFLDNWPLLEEWFTQPKNILTKVNAEIDEALLCQKVKEIFATETVNKKIKVEKTLEEKETEKKAGAPPAEPPAMSPPLSSEAEKDKELHQAKTPGKGKTQSETQHGKQESQPEGKGKKVSPKGKAQGGKVSVKKSPVGSAEVSPTPTAPPPPKAGTEEWVYVNEPIPEELPSFLVPYWELIEKSYINHIKTVLRHLRERQHNVLSYLYETRTSFQEFLRRPDHKQDFVSQWQADFNSVPEDLWEDEETKAELHQRVNDLRDRLWDICEARKEEAEQERLDIINESWLQDSIGITMNHFFSLMQAEVNRFQDTKRLLQDYYRAMESKIPLEDSKKFTRVPLVQLDGKEISESQLRIPLVPRISNSPENSAVKPKVGTFLKGRSDPPLEVLEANFEIDEKILLDTWQQASLAISNMVAAEVHQRLTEEEKEPPQLDSKEKSPQSGANKKAKKEKEAPKKKKTDKKGKGKSSPVAEVSPVTVTPEEMAEMEKRNELRLRIKEEHLAALQTEEQAAQFRLELIKLKALSVLEDLVTKVIDVYRLMEKWLGKRYLNEMASIQKLTELARYHIETATKIQNEIYLSQEDFYINGDIKVFPDPSPPTRPPPVEREENGTLTIEQLDNLRDQFLDMAPKGIIGNKAFSDILLDLITLNLGTNNFPSSWMHLSQLDLQEITSLLTVNTEFVDWRKFLMVTAMPWPMALEDELLDTLQRFKALDEAQTGTITYEQYKQAGLWFSGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADCEKEPPQLDYTQMLLYFACHPDTLEGVYRALSVAVGTHIFRQVETPMLMAEKTSISTVSPIEEFPETEESSAKEDRELKEEKDDQKEEEIPENANTEKISMETLLKVFGGGNEVLDANRFASYLKSENIYAENFIKTFQDLGARNLEPIAVNILLKHPYIQDLIANYVDYKFPDIKMILQRSEHAQGSDGERSPSRLTDEKK

Target ORF information:

RefSeq Version	NM_001305042.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm flagellar 2 (Spef2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305042.1

ORF Insert Sequence:

ATGTCGGAAA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA GGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCTAAT GGCTATCTAA TTGGAGAAGT TCTGTTCAAG 
TTTGAACTTC AGAGTGATTT TGCAGAATTT TCAGAGAGCA GGGGTTCAAC AGCCAAGCTT 
AACAATTTCT CCCGCTTGCA ACCAACACTT CACCTGCTGG GTTTGCAGTT TGATCAGAAT 
GTGGCTCAAA GCATCATCAC AGAGAAGCCT GGGGCAGCAA CCAAACTTCT GTATCAGTTG 
TACATTGCTC TTCAGAAAAA GAAGAAAACA GGGCTGACTG GACTAGAGAT ACAAACCATG 
CAACCCCAGA CAAACTTGAG GCTTCAGACC CTCAAAAGTG AGGCTTTTCG AGAGAGACTT 
AAAAACCTGA TACCACGGCA AACCGATTTC AATCTGATGC GGGTTACATG CAGGTTTCAA 
GAAAAGTGTA AACAAATGAA AGAAGATCTT GCCCGAATGA ATTTTGAGAA GTTTGAAAAA 
ATTCAAAAAC TTGAGGAAGA GCAAAGACAC TTTAATATCG AAAAGCAACG CTTAAACAGA 
AGACGACAAA ATGAGATAAT GGCCAAAATC CAGGCAGCCA TTATACAGAT CCCCAAGCCT 
GAATCCAACC GTACTTTGAA AGCTATTGAG GCTCAAAAAC TGATGAAAAA GAAAAAGGAG 
GCAGAGGACG TGGCCAATGA GATTAAGAAA TTTGAAGCAC TAATTAAAAA GGATCTCCAA 
ATTAAAGAAA GTGTATCCAA GACTTCTTTA GAAACAACAG ACCAGACAAC TGCAGAACTG 
CTCAACACTT ATTCAGATGA TGACTACATT AAAAAGATCC AGAAACGCCT AGAAGAAGAC 
GCTTTCGCAC GCGAGCAAAG GGAAAAAAGA CGACGCAGAT TGTTGATGGA TCAGCTAATG 
GCCCATGAAG CACAGGAGGA GGCCTATCGG GAAGAGCAGC TCATTCACCG GCTCATGCGC 
CAGTCCCAGC AGGAGCGCAG GATTGCTGTG CAGCTCATGC ATGTTCGCCA CGAGAAGGAA 
GTTTTGTGGC AAAATAGAAT TTTCCGAGAG AAACAATTTG AAGAAAGACG ACTTAAGGAT 
TTCCAGGATG CTCTTGATAG AGAAGCGGCT CTTGCCAAAC AAGCCAAGAT TGATTTTGCA 
GAACAAACCT TGAGGGAGAA GGAAATTCAT GAAAAGATCT CTGTGGAAAG AGCCCAACAG 
CGTTATAAGA AGCATTATGG GATATGTGCA GAGATTTTGG ATCAAATGCT AGATTTGTGC 
ACGAAGGTGG CAGACTATAG GTTGCTGACA AATAATCTTA TTCCACATAA GATGATGCAC 
GATTGGAAAG AACTGTTTTT TAGTGGAATT CCCATATATG AACAGACTTC TCTTACACAT 
GGACAAACAG AACCCACTGA GGATCACCGT GCAGAAGTGA AGAAAAGGAA CTTACTCGAT 
AGCAAAGATT ATGAAGATTA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAGGACATG 
GTTAATAGTT CACCACCCTC CAACAATAGC ATTTTGGGTC ACGTGCTTCT GAGATTAATT 
GAAAAAGCTG ATCCATCTGC AAGTAATGCT GAAGCCACTG AATTACCTTC ACTTGCGGTC 
AAGGGTTGTA TACTGGGGAA GACACTCAGT GGAAAAACTA CTGTTCTAAA GTCTCTACAA 
AATGACTTTC CTGTTCATGT GCTTTCTATT GATACTCTTG TCCAAGAGGC TATCCAGGCG 
TTTCATGAAA GACAAAAAAG CGGTAAGACA CCACCAACTC AGGAAGATGA TAAAAGAGAT 
CCTGTAGTGA ACCAGGAAAA AGTGAGCAAA ACTCAGGATA AAAATGTGTT AGCAGTTAGT 
CCAGTTCCAG GGGACAGAAC ATCCCAAAAA GAAGGTGTCA AGATTAATGA ATTTGAACAG 
TTCAGATCAA GTGATAGCTT TTTATCACTT AGCATGCGAG CTCAACTTGG TGCAAAGTCA 
GAGTTGATGC TTAGAAGAGG AAAGAGCATT CCAGACATAC TTCTGGTCAG CATCCTGGTA 
AATGCTATAA AGGAGATACC TGTGGACCAA AGCTGGGTTC TTGATGGATT TCCAATAACA 
TTAAATCAAG CCAAACTCCT GGAGGAAGCA CTTACAGGGT ACAAAAGAAA ATTCCTACAG 
TTAAAGAAGA AGAAAGAACA AATGCCCACA TTGGCCCTAG ACCCCTCAAC TTCCACAGAG 
GTCTCTCTTC TCCCATCTGC ACTTGATTTT GTCATATTGC TAGATATTTC CGATAACTCA 
TCGTTGGCTC GAACTAATGA TATCATAGCT AAAGAAATCT CACATGAAAT TTCTCATGAA 
AATGTTGGCC GTCCTGGAAC TGGTACAAGC CAAGATAATA AGAGCGAGGA CCGGAACTTA 
AGAGACCACA TACAGCACAG AATTGTTGGC TTCTTGGACA ACTGGCCACT GTTGGAAGAG 
TGGTTTACAC AGCCAAAAAA CATTCTGACG AAAGTCAATG CTGAAATAGA TGAAGCGCTC 
TTATGCCAAA AAGTAAAAGA AATTTTTGCT ACTGAAACAG TAAACAAAAA GATCAAAGTT 
GAAAAGACAC TGGAAGAAAA GGAAACTGAG AAAAAGGCAG GAGCTCCCCC GGCTGAGCCT 
CCCGCTATGA GCCCTCCCCT TTCTTCTGAA GCAGAAAAAG ACAAGGAGCT TCATCAGGCA 
AAAACTCCTG GGAAAGGAAA GACACAATCA GAAACCCAGC ATGGTAAGCA AGAATCTCAA 
CCTGAAGGAA AAGGAAAGAA AGTTTCTCCT AAAGGAAAAG CACAAGGAGG CAAAGTTTCA 
GTAAAGAAAT CACCGGTTGG CTCAGCAGAA GTATCACCCA CACCAACAGC ACCACCGCCA 
CCTAAGGCAG GAACAGAAGA ATGGGTTTAT GTGAATGAGC CTATACCGGA GGAACTCCCT 
TCATTTTTGG TACCTTACTG GGAACTGATA GAGAAATCCT ACATCAACCA CATCAAAACA 
GTACTTCGAC ATCTCAGAGA GAGACAGCAC AATGTGCTCT CTTATTTATA TGAGACTAGG 
ACAAGTTTCC AGGAGTTTCT ACGGCGTCCA GATCACAAGC AAGATTTTGT ATCTCAGTGG 
CAGGCTGATT TCAACTCTGT TCCTGAAGAC CTGTGGGAAG ATGAGGAAAC AAAGGCTGAG 
CTACACCAAA GAGTAAATGA TCTGCGAGAC CGCCTATGGG ACATCTGTGA AGCGAGGAAG 
GAAGAGGCGG AACAGGAGAG GCTTGATATC ATTAATGAGA GCTGGCTGCA GGACTCTATC 
GGAATCACAA TGAATCATTT CTTTTCCCTG ATGCAGGCAG AAGTGAACCG TTTTCAAGAT 
ACCAAAAGAC TCCTTCAAGA TTATTACAGA GCAATGGAAT CCAAAATCCC TCTGGAAGAC 
AGTAAGAAAT TTACTCGGGT CCCATTGGTT CAGCTTGATG GTAAAGAAAT TTCAGAAAGC 
CAGCTCAGAA TTCCTCTAGT TCCTCGAATA TCCAATTCTC CAGAAAATTC TGCCGTCAAG 
CCAAAGGTGG GGACATTTCT GAAGGGCAGA TCTGATCCTC CTTTAGAAGT CCTGGAAGCC 
AACTTTGAAA TAGATGAGAA GATTTTATTG GACACCTGGC AGCAGGCTTC ATTAGCCATC 
TCTAACATGG TGGCTGCTGA AGTTCACCAA AGGCTCACAG AGGAAGAAAA AGAACCACCC 
CAATTAGATT CCAAAGAAAA GTCTCCTCAG TCAGGTGCAA ATAAAAAAGC CAAGAAGGAG 
AAAGAAGCAC CCAAGAAGAA AAAGACAGAC AAGAAAGGAA AAGGCAAATC ATCCCCCGTG 
GCAGAAGTCA GTCCAGTCAC AGTCACACCT GAGGAAATGG CTGAGATGGA AAAGAGGAAT 
GAACTGAGGC TCAGGATAAA GGAAGAACAT CTTGCTGCCC TGCAGACAGA AGAACAAGCC 
GCACAATTTC GACTTGAGCT AATAAAGTTG AAAGCTTTGT CCGTCCTGGA AGATTTAGTA 
ACAAAGGTGA TTGATGTCTA CAGGCTCATG GAAAAATGGC TTGGTAAGAG ATATTTGAAT 
GAAATGGCCA GTATACAGAA ATTGACCGAA TTAGCTCGCT ACCACATTGA AACAGCTACA 
AAAATTCAAA ATGAGATTTA CTTAAGCCAA GAAGACTTCT ATATCAATGG AGACATAAAA 
GTCTTCCCAG ATCCTTCTCC ACCAACCCGC CCACCACCAG TAGAAAGGGA AGAAAATGGT 
ACCCTAACAA TCGAACAGCT TGATAATCTT CGAGATCAGT TCTTAGACAT GGCACCTAAA 
GGCATAATCG GAAATAAAGC CTTCAGTGAC ATTCTGCTTG ATTTGATTAC CCTAAACCTT 
GGAACCAATA ACTTCCCTAG CAGCTGGATG CACCTCAGCC AACTGGACTT GCAGGAGATC 
ACATCTTTAT TAACAGTCAA CACAGAGTTC GTGGACTGGA GGAAGTTCTT GATGGTCACA 
GCCATGCCGT GGCCCATGGC CCTGGAGGAT GAGCTTCTGG ACACCCTGCA GAGGTTTAAG 
GCCCTGGATG AAGCACAGAC AGGCACCATC ACCTACGAAC AGTATAAGCA GGCTGGTCTA 
TGGTTTTCAG GAGATGAAGA TATAAAAATT CCAGAAAATC CCCTTGAACC CTTGCCATTC 
AATCGACAGG AGCATCTTAT AGAGTTCTTC TTTAGGCTGT TTGCGGACTG TGAGAAGGAG 
CCACCTCAGC TTGACTACAC TCAGATGCTG CTGTATTTTG CGTGCCACCC AGACACTTTG 
GAAGGAGTCT ACAGGGCCCT CAGTGTGGCT GTGGGAACTC ACATCTTCCG CCAGGTTGAA 
ACTCCGATGC TGATGGCAGA AAAGACCTCC ATCTCCACTG TGAGTCCCAT TGAGGAATTC 
CCTGAGACTG AGGAAAGTAG TGCAAAAGAG GACCGGGAAC TGAAGGAGGA GAAAGATGAC 
CAAAAGGAAG AAGAAATTCC CGAAAATGCA AACACTGAAA AGATTTCCAT GGAAACACTG 
CTCAAAGTGT TTGGAGGAGG AAATGAAGTA CTGGATGCCA ACAGATTTGC CAGCTACCTA 
AAATCAGAGA ATATTTATGC AGAGAATTTC ATCAAGACTT TTCAGGACCT GGGTGCCAGG 
AACCTGGAGC CAATTGCAGT TAATATTCTC TTGAAGCATC CTTACATTCA GGACCTGATT 
GCAAATTATG TAGACTATAA ATTTCCTGAC ATTAAAATGA TTCTTCAAAG GAGTGAGCAT 
GCACAAGGCA GTGATGGAGA AAGGTCACCC TCAAGACTTA CAGACGAAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cilia-related protein SPEF2 regulates osteoblast differentiation.
Scientific reports8(1)859(2018 Jan)
Lehti MS,Henriksson H,Rummukainen P,Wang F,Uusitalo-Kylm?l? L,Kiviranta R,Heino TJ,Kotaja N,Sironen A

SPEF2 functions in microtubule-mediated transport in elongating spermatids to ensure proper male germ cell differentiation.
Development (Cambridge, England)144(14)2683-2693(2017 07)
Lehti MS,Zhang FP,Kotaja N,Sironen A

Strain-dependent brain defects in mouse models of primary ciliary dyskinesia with mutations in Pcdp1 and Spef2.
Neuroscience277552-67(2014 Sep)
Finn R,Evans CC,Lee L

Enhanced response to pulmonary Streptococcus pneumoniae infection is associated with primary ciliary dyskinesia in mice lacking Pcdp1 and Spef2.
Cilia2(1)18(2013 Dec)
McKenzie CW,Klonoski JM,Maier T,Trujillo G,Vitiello PF,Huber VC,Lee L

Loss of SPEF2 function in mice results in spermatogenesis defects and primary ciliary dyskinesia.
Biology of reproduction85(4)690-701(2011 Oct)
Sironen A,Kotaja N,Mulhern H,Wyatt TA,Sisson JH,Pavlik JA,Miiluniemi M,Fleming MD,Lee L

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Spef2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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