spef2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	spef2
Entrez Gene ID	100491905
Full Name	sperm flagellar 2
General protein information	Preferred Names sperm flagellar 2 Names sperm flagellar protein 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012967689.2	XP_012823143.1	sperm flagellar protein 2 isoform X3
XM_031894698.1	XP_031750558.1	sperm flagellar protein 2 isoform X1
XM_012967692.2	XP_012823146.1	sperm flagellar protein 2 isoform X4
XM_012967670.2	XP_012823124.1	sperm flagellar protein 2 isoform X1
XM_031894707.1	XP_031750567.1	sperm flagellar protein 2 isoform X3
XM_004910428.2	XP_004910485.1	sperm flagellar protein 2 isoform X2
XM_031894702.1	XP_031750562.1	sperm flagellar protein 2 isoform X2
XM_018090829.1	XP_017946318.1	sperm flagellar protein 2 isoform X5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPEF2	XP_546353.2
Gallus gallus (chicken)	SPEF2	XP_003643024.2
Danio rerio (zebrafish)	spef2	NP_001121704.1
Homo sapiens (human)	SPEF2	NP_079143.3
	LOC703498	XP_001091802.1
Mus musculus (house mouse)	Spef2	NP_796097.2
Pan troglodytes (chimpanzee)	SPEF2	XP_001143539.2
Bos taurus (cattle)	SPEF2	XP_002696427.2
Rattus norvegicus (Norway rat)	Spef2	NP_072142.1
Xenopus tropicalis (tropical clawed frog)	spef2	XP_002940003.2

Gene Ontology

The following spef2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spef2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa03384	XM_004910428.2 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35202	XM_012967670.2 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35203	XM_012967689.2 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35204	XM_012967692.2 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa42251	XM_018090829.1 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49220	XM_031894698.1 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49221	XM_031894702.1 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa49222	XM_031894707.1 Latest version!	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spef2 ( XM_004910428.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910485.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa03384
Clone ID Related Accession (Same CDS sequence)	XM_004910428.2
Accession Version	XM_004910428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA 
GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT 
ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA 
AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG 
GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT 
ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT 
ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC 
CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA 
TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT 
ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG 
CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG 
GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG 
CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG 
ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC 
CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC 
TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG 
CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG 
GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA 
ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA 
GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT 
GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT 
AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910485.1
CDS	134..5392
Translation	MSEIICQWLNEDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKVPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTNLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKQSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKEAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKVLLPLSHENKR

Target ORF information:

RefSeq Version	XM_004910428.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910428.2

ORF Insert Sequence:

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA 
GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT 
ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA 
AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG 
GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT 
ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT 
ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC 
CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA 
TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT 
ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG 
CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG 
GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG 
CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG 
ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC 
CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC 
TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG 
CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG 
GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA 
ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA 
GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT 
GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT 
AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_012967670.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823124.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.

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CloneID	OXa35202
Clone ID Related Accession (Same CDS sequence)	XM_012967670.2
Accession Version	XM_012967670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA 
GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT 
ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA 
AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG 
GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT 
ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT 
ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC 
CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA 
TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT 
ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG 
CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG 
GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG 
CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG 
ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC 
CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC 
TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG 
CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG 
GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA 
ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA 
GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT 
GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT 
AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823124.1
CDS	124..5382
Translation	MSEIICQWLNGDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKVPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTNLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKQSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKEAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKVLLPLSHENKR

Target ORF information:

RefSeq Version	XM_012967670.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967670.2

ORF Insert Sequence:

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA 
GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT 
ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA 
AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG 
GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT 
ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT 
ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC 
CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA 
TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT 
ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG 
CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG 
GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG 
CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTG 
ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC 
CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC 
TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG 
CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG 
GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA 
ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA 
GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT 
GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT 
AAGGTACTTT TACCTTTGTC TCATGAAAAC AAGAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_012967689.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823143.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa35203
Clone ID Related Accession (Same CDS sequence)	XM_012967689.2
Accession Version	XM_012967689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT 
GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA 
TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA 
AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG 
AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT 
AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA 
AAGGCTCTCA CTGGCTCACA CACTAATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT 
GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT 
GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA 
ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG 
AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC 
CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT 
GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC 
GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA 
GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT 
TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA 
TCTCCAAGAG AATCTCGAAA ACAGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA 
GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA 
AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA 
CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA 
ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC 
AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT 
ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT 
CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA 
GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC 
AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT 
CACACCGGGA TACTTATTAA CCACTTTTTC TCTCTGATGC AGGCGGAAGT TGATCGCTTC 
CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT 
GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG 
GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA 
CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA 
GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT 
GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG 
GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT 
GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG 
AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823143.1
CDS	124..5379
Translation	MSEIICQWLNGDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTNLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKQSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKEAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKVLLPLSHENKR

Target ORF information:

RefSeq Version	XM_012967689.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967689.2

ORF Insert Sequence:

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT 
GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA 
TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA 
AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG 
AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT 
AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA 
AAGGCTCTCA CTGGCTCACA CACTAATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT 
GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT 
GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA 
ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG 
AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC 
CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT 
GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC 
GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA 
GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT 
TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA 
TCTCCAAGAG AATCTCGAAA ACAGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA 
GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA 
AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA 
CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA 
ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC 
AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT 
ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT 
CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA 
GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC 
AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT 
CACACCGGGA TACTTATTAA CCACTTTTTC TCTCTGATGC AGGCGGAAGT TGATCGCTTC 
CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT 
GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG 
GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA 
CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA 
GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT 
GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG 
GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT 
GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG 
AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_012967692.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823146.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X4, mRNA.

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CloneID	OXa35204
Clone ID Related Accession (Same CDS sequence)	XM_012967692.2
Accession Version	XM_012967692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012823146.1
CDS	124..5379
Translation	MSEIICQWLNGDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKVPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTNLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKQSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKVLLPLSHENKR

Target ORF information:

RefSeq Version	XM_012967692.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967692.2

ORF Insert Sequence:

ATGTCGGAGA TTATCTGCCA GTGGCTTAAT GGAGACCTCA AACTCTCCAA GCGTGTGGAG 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTAAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACAGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCTCTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTGACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
GTACTTTTAC CTTTGTCTCA TGAAAACAAG AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_018090829.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946318.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X5, mRNA.

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CloneID	OXa42251
Clone ID Related Accession (Same CDS sequence)	XM_018090829.1
Accession Version	XM_018090829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAC AGCACGGAGT GGCTACCCGG CTTCTGTATC AGTTGTTCAT AGCATTGCAG 
AAGAAAAAGA AGGCAGGATT GACAGGAGTA GCCATGGAGA CATTGAGACC AGCTGCTCCA 
GCAAAGCTTC AGAGCATTGG AACTGAGATG TACAGAGAGC GTTTGAAAAC CATAATCCCC 
CGCCAAAGTG AAATCAGCCT GCAGAGGGTG TCAGAACAAT TTGACCTGAA AGCAAAAGAA 
ATCGAAGACA AAGTTGCACA GATTCAAAAT GATGAACTTC GGAAAATACA GAAAATACAA 
GAAGAGCTAA GAATTCAGGA TATTGAAAAG CTGCGACGAG CTAGAAGGAG ACAAACCGAA 
ATAATGGCAC GAATTCAAGC AGCAATTGTT CAGATTCCAA AACCACCTCA AAACCGCACA 
TTGAAGGCCA TCGAAGCACA AAAGATGCTG AAAAAGAAGA AAGAAGCTGA GGAAGTTTAT 
ACAGAAATCA CAAAGTTTGA AAAAGGAGTG AAAGATGGAT CTGTTGGAAG CTTGTCTGCT 
AATACCAGGG GCCCAACACT CCAGAGATTA AGGTCATCAG TTAACATTGG CACTGCTTTG 
CTAAAACTGG ATACTAATGA TGATTACGTA AGGAAAATTC AGAAGCGATT AGAGGAGGAT 
TCTGTAGCAC GTGAGCAGCG CGAAAAGAGA CGTCGGCGGA TTCTTATGGA ACAGCTTGTG 
GGTCATGGGG CACAAGAGGA AGCTTTCCGG GAGGAACAAC TGATAAACCG ATTAATGAGA 
CAGTCCCAGC AAGAGCGGAG AATTGCAGTT CAGCTTATGC ACGCGCGCCA TGAAAAGGAA 
GTTATGCAAC AGAACCGAAT GTTCCGGGAG AAGCAATATC AGGAGAGGCG CGAGAGGGAG 
TTCCAAGAAG CTCTTGACAG AGAAGCGGCG TTGCAGAAAC AAGCGATGCT CGACCACAAA 
GAAGAAATAC GCAAAGTGCA GGAGCTCCAT GACCAGATAG CAGCGGAGCG TGCAGAGGCT 
CGCTATAAAA AGCACTATAA CCTGTGCCAG GAAATCATGA ATCAGATTTT AGACTTTGTT 
ATTAAAACAG GGGAATACAG AGAGCTAACA AACAGACTTA TACCTGTTAA ATTGATGCGG 
GAGTGGAAAG AACTATTCTT CGCTGGTTAC CCGCTTTACG ACAAAGCACC TATAGATGCC 
CTCCCTTCCG ACCCAACTGC AGAGCAGCTG CAAGAAACAG AAAAGCAGAG TCTGTTAGAT 
GAGAAGGATT ATGAAGAATA CAAGACCATG ACAGGGGAAT GGGGCCCACC CGAAGCCAGC 
AGTGTTAAAG CACCTGCACC GAACAACAAC ATATTGGGCT ATGTGGTTCA TAGACTGTTG 
GAAATTGTCA GCCCCCCATT GGCACCCACA CCGCCACCTG TGTTTCCCCC CTTCCCCATC 
AAGGGTTGTG TTCTTGGGAA GATTTACTCT GGGAAGAGCA CGTGCCTGAA ACATCTGGCA 
CAGGTGTATA GCATCCAAGT TCTCTCTGTG GACACCCTTC TGGAGGAAGC TATTCAAGCA 
TTTAATGACG GGGAACTGGA AAAGACCGAG GATGTTCTGA GGCCCATACA AGAAGGAGCA 
GATAAAGAGA ACAAGGTACC CATAGACAGT CCTGCTCCTG TTACTAATGG CGAAGAGATA 
CCTGTTGTGG GAAGTGGCAG CAAGCCTGCA TCTGCAGAAG CCCGTGAATC AGATAAAGAG 
TTATCTCTAC ATCAAATATT AAAGCCTGCA ATAGAAGATA CTGTGCCAAA GCTCTCTGCA 
CGGGCACAGC TTGGTGGACT GGCAGAGACA CATTTAAAGA GTGGGAAGAG TGTCCCAGAT 
GAGCTGCTTA TCAGTATACT TGTTGAAGCC ATCAATCGCA TCCCAGCTAA CACAGGGTGG 
ATCATGGAGG GATTCCCTAT GACGGAAACT CAGGCCACAT TATTTGAAAA GGCTCTCACT 
GGCTCACACA CTAATTTGAA TGGAATAAAG GGGAATAATA ACAAGTTTGC CTCTTTAGTA 
ACAGATCCAG CAGCACCAAA GGATCTCCCT GTGGCTCCAT CTGCTCTTGA CTTTGCTGTG 
TTAATAGAAA TATCAGACAA TGAAGTGTTA CAGCGCATGT CTACAATAAT CAGTGAAATA 
GATAAATCTG GAGAGAAAAT TGATAGTCCT CCCTGTATTC AAACTACGAG AATGGAAGCA 
GATCAAACAA TGGACCAGAT ACAGCACAGA ATTACAGGAT TTCTTGACCA CTGGAAGAAT 
TTAGAAATGT GGTTTTCAGA GCAGCAGAAT ATTCTAATCA AAGTTAATGG GGAAGTGGAA 
GTTGGCGTTC TGTGCAAGAG AATAGAAGAA GTATACTTGA CAGCTCTCGT CAATAAGCAA 
AACCGAGGCA AAAAAAACGA AAAGAAAGAA GATCTCCCAC CACCAGAAGT AGCCGCACCA 
ACACCGCCAC CGCCATCTCC TCCTCCAGCT CCAGAAGCCA CTAACAATTT ACGACCTCCA 
TCAGCTTCAA GCAAGGAATC AGCAAAAAAA AGCAGGAAAG GCTCCAAATC TCCAAGAGAA 
TCTCGAAAAC AGTCTGACAC CTCGAAGGAC AAGAAGGACA AGAAACCAGA GACACCTAAA 
GGAAAAGACT CTCGAAAATC TGGTTCACCA CGTGGAAGGT CTCCTGGAAA GAAGGCTAAG 
TCTGTACCTG CCAGCCCTGA ACCAGTCGCT GTCACTCCTG TTGGACCACC ACCCATCCAG 
CCTGGATCCC CTGAATGGGT GTACGTAAAT GAGCCACTTC CGAAAGAAAT CCCAGCATTC 
CTTGTACCAT ATTGGAAGAC TATAGAAAAT GTCTATGGAA TGACCATCAA AACTGTGCTG 
AGGAATCTGA GGCAGGAACG ACTAACTGTG ATTCACTATT TATATGATAT CAGGGTTCAT 
TTGAAGGATT ACTTGAAGCG GCCGGATCAC AAGCAGGAAT TCGTGTCTCA GTGGCAGTCG 
GACTTTAATT CTACCGCAGA CGACTTGTGG GAGGACGAGG AAACAAAAGC AGAGCTTCAT 
CAAAGAGCTG ATGATCTCCG GGACCGTCTG TGGGACATAT GTGACAACAG GAAGGAGGAG 
GCAGAACAAG AGCGCACAGA CATTATGAAT GATGGGTGGC TACAGGATCA CACCGGGATA 
CTTATTAACC ACTTTTTCTC TCTGATGCAG GCGGAAGTTG ATCGCTTCCA AGACACCACA 
AGACTTCTCA GTGATTACTA CCAAGGAATG GAGGGACAAG CCCCATCTGA GGCCAGCCAT 
ACCTTTTCCC GTATCCCACT GCTGGACATT GCTAACTCAA GCACGCAGGG GGATTCTGAG 
AACCCTAAAA GAATTCCTCT TATACCCCGA AGAGCCCAGT CACCTGAACA GAACACAGGC 
AAACAGAAAG CCAAAAGTCA AGCCAAGGCA AAGGAGGAGC CCCCCACAGA CCACCTTGGG 
CAGATCTCCG AGGCTGATGA AAAACTTATC ACTGACACCT GGCAAACTGC TGTTTCAGCC 
ATTGCAAACA TGGTGTCGAA TGAAAAACAG TCCAAAGAAG CAGAAGAGGA AAAAGAAAGG 
CAACTGATGG AAATGAAGGA AAAAGAGAGA GCGAAAGCTT CACAAGCTGC ATCTCGGGCT 
AGTGGCAAAG GAGGGAAAAA GAAAGCGCCG CCAAAGTCGC CAAACAGGAA AAAAGAAGCA 
AAGTCCCCAG GCCCTGCAGA CCCCACACCT TCTCTTCAGA CCCTAGAAGA CAGTACTGAG 
TTGCAAAAGA AGCAGGAGCT AAAGATGAAG TTGAAGCAGG AGCATATTGC TGCTTTAGAG 
TATGAAGAGA TGGCTGCTAA CTTACGGCTG GAAGTTATTA AAACAAAAGC CTTAGAAGTG 
TGCCAGGATA TTGCATCGAG AGCTGAGAGT GCCTACAAAG ATATGGAAAT GTGGATGGGA 
GCAAGATTCC TGGCAGAAAT GGCAAGCGTG GAGCAACTCA TCCATATTGC TCGTCACCAT 
ATTGAAACGG GCACCAAAAT AAAGTATCAG CTTGTCCTGG AGAATACAGA TTTCTACATT 
AATTCAGATG TACAAGTGGT GCCCGATCCT CCGCCACCTT CTCGCCCTCC TGCAGTAGAG 
ATATCAGCCA ATGCGACACT GACTGTCGCT CAGCTCCACA GATTATACCA GCAGTTTGCT 
CAGATCGCTC CTGAAGGACT AATATCCAAT AAAACGTTTA TCGATATTCT CCTGGATATT 
ACATCAGCGA GACTTGGAAA CGATGCCCTC CCTGATTCGT GGATGCAGCT TACACTTCCA 
GAGATAAAAG AAATGACATC TGAATTATCT CAGAACTCTG ATGTGGTAAA CTGGCGCCTA 
TTCCTGCTGT CAGCATCTCT GCCATGGCCG TACCCCTCCG TAACGCAGCT TCTCAGCACT 
CTGCAGAGCT TCAAGGCTGT GGATCATAAT GGCTGCGGCA CACTGACCCA CGAAGGTTTC 
AGTCAGGCTG AGCTGTGGTT TCATGGAAAT GCAGAGGAAC CTGTCCCTGA AAATCCCACA 
GAGCCTTTGC CATTTAACAG ACCGGCCCAT CTAATAAAGT ATTTCTTTGA CTTGTTTGCT 
GACCCCCGGG GAATCCCCCC CCAGCTGAAT TACACAGAGA TGCTGCTGTA TTTTGCCTCA 
CACCCCGACC CTATGGAAGG CTTCTATCGG GCGCTGAGTG TTACGGCGGG GAAGCCTATT 
CTGAAGGCAC CAGGAAATAA TGTTTTGCTT GAGTCTCTCC CAGCAATGGA GGCCATTCAG 
CAGATGAAAG AACCAATCAG CAGCAATGAC ACAGTGGGGA CACGAGGCTT GGGAAAGGCA 
GCGGTCACAT TACATGAAGT GGTGAGAGTG CTGCAGCACG CTGCTGGGAC GGGTGAAGAT 
AACCATAGGT TTTGCCTGGA AGAAAATGGC AGGAACAATA TTTATAAGGT ACTTTTACCT 
TTGTCTCATG AAAACAAGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946318.1
CDS	229..5232
Translation	MSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKVPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTNLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKQSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKEAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKVLLPLSHENKR

Target ORF information:

RefSeq Version	XM_018090829.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090829.1

ORF Insert Sequence:

ATGTCAGAAC AGCACGGAGT GGCTACCCGG CTTCTGTATC AGTTGTTCAT AGCATTGCAG 
AAGAAAAAGA AGGCAGGATT GACAGGAGTA GCCATGGAGA CATTGAGACC AGCTGCTCCA 
GCAAAGCTTC AGAGCATTGG AACTGAGATG TACAGAGAGC GTTTGAAAAC CATAATCCCC 
CGCCAAAGTG AAATCAGCCT GCAGAGGGTG TCAGAACAAT TTGACCTGAA AGCAAAAGAA 
ATCGAAGACA AAGTTGCACA GATTCAAAAT GATGAACTTC GGAAAATACA GAAAATACAA 
GAAGAGCTAA GAATTCAGGA TATTGAAAAG CTGCGACGAG CTAGAAGGAG ACAAACCGAA 
ATAATGGCAC GAATTCAAGC AGCAATTGTT CAGATTCCAA AACCACCTCA AAACCGCACA 
TTGAAGGCCA TCGAAGCACA AAAGATGCTG AAAAAGAAGA AAGAAGCTGA GGAAGTTTAT 
ACAGAAATCA CAAAGTTTGA AAAAGGAGTG AAAGATGGAT CTGTTGGAAG CTTGTCTGCT 
AATACCAGGG GCCCAACACT CCAGAGATTA AGGTCATCAG TTAACATTGG CACTGCTTTG 
CTAAAACTGG ATACTAATGA TGATTACGTA AGGAAAATTC AGAAGCGATT AGAGGAGGAT 
TCTGTAGCAC GTGAGCAGCG CGAAAAGAGA CGTCGGCGGA TTCTTATGGA ACAGCTTGTG 
GGTCATGGGG CACAAGAGGA AGCTTTCCGG GAGGAACAAC TGATAAACCG ATTAATGAGA 
CAGTCCCAGC AAGAGCGGAG AATTGCAGTT CAGCTTATGC ACGCGCGCCA TGAAAAGGAA 
GTTATGCAAC AGAACCGAAT GTTCCGGGAG AAGCAATATC AGGAGAGGCG CGAGAGGGAG 
TTCCAAGAAG CTCTTGACAG AGAAGCGGCG TTGCAGAAAC AAGCGATGCT CGACCACAAA 
GAAGAAATAC GCAAAGTGCA GGAGCTCCAT GACCAGATAG CAGCGGAGCG TGCAGAGGCT 
CGCTATAAAA AGCACTATAA CCTGTGCCAG GAAATCATGA ATCAGATTTT AGACTTTGTT 
ATTAAAACAG GGGAATACAG AGAGCTAACA AACAGACTTA TACCTGTTAA ATTGATGCGG 
GAGTGGAAAG AACTATTCTT CGCTGGTTAC CCGCTTTACG ACAAAGCACC TATAGATGCC 
CTCCCTTCCG ACCCAACTGC AGAGCAGCTG CAAGAAACAG AAAAGCAGAG TCTGTTAGAT 
GAGAAGGATT ATGAAGAATA CAAGACCATG ACAGGGGAAT GGGGCCCACC CGAAGCCAGC 
AGTGTTAAAG CACCTGCACC GAACAACAAC ATATTGGGCT ATGTGGTTCA TAGACTGTTG 
GAAATTGTCA GCCCCCCATT GGCACCCACA CCGCCACCTG TGTTTCCCCC CTTCCCCATC 
AAGGGTTGTG TTCTTGGGAA GATTTACTCT GGGAAGAGCA CGTGCCTGAA ACATCTGGCA 
CAGGTGTATA GCATCCAAGT TCTCTCTGTG GACACCCTTC TGGAGGAAGC TATTCAAGCA 
TTTAATGACG GGGAACTGGA AAAGACCGAG GATGTTCTGA GGCCCATACA AGAAGGAGCA 
GATAAAGAGA ACAAGGTACC CATAGACAGT CCTGCTCCTG TTACTAATGG CGAAGAGATA 
CCTGTTGTGG GAAGTGGCAG CAAGCCTGCA TCTGCAGAAG CCCGTGAATC AGATAAAGAG 
TTATCTCTAC ATCAAATATT AAAGCCTGCA ATAGAAGATA CTGTGCCAAA GCTCTCTGCA 
CGGGCACAGC TTGGTGGACT GGCAGAGACA CATTTAAAGA GTGGGAAGAG TGTCCCAGAT 
GAGCTGCTTA TCAGTATACT TGTTGAAGCC ATCAATCGCA TCCCAGCTAA CACAGGGTGG 
ATCATGGAGG GATTCCCTAT GACGGAAACT CAGGCCACAT TATTTGAAAA GGCTCTCACT 
GGCTCACACA CTAATTTGAA TGGAATAAAG GGGAATAATA ACAAGTTTGC CTCTTTAGTA 
ACAGATCCAG CAGCACCAAA GGATCTCCCT GTGGCTCCAT CTGCTCTTGA CTTTGCTGTG 
TTAATAGAAA TATCAGACAA TGAAGTGTTA CAGCGCATGT CTACAATAAT CAGTGAAATA 
GATAAATCTG GAGAGAAAAT TGATAGTCCT CCCTGTATTC AAACTACGAG AATGGAAGCA 
GATCAAACAA TGGACCAGAT ACAGCACAGA ATTACAGGAT TTCTTGACCA CTGGAAGAAT 
TTAGAAATGT GGTTTTCAGA GCAGCAGAAT ATTCTAATCA AAGTTAATGG GGAAGTGGAA 
GTTGGCGTTC TGTGCAAGAG AATAGAAGAA GTATACTTGA CAGCTCTCGT CAATAAGCAA 
AACCGAGGCA AAAAAAACGA AAAGAAAGAA GATCTCCCAC CACCAGAAGT AGCCGCACCA 
ACACCGCCAC CGCCATCTCC TCCTCCAGCT CCAGAAGCCA CTAACAATTT ACGACCTCCA 
TCAGCTTCAA GCAAGGAATC AGCAAAAAAA AGCAGGAAAG GCTCCAAATC TCCAAGAGAA 
TCTCGAAAAC AGTCTGACAC CTCGAAGGAC AAGAAGGACA AGAAACCAGA GACACCTAAA 
GGAAAAGACT CTCGAAAATC TGGTTCACCA CGTGGAAGGT CTCCTGGAAA GAAGGCTAAG 
TCTGTACCTG CCAGCCCTGA ACCAGTCGCT GTCACTCCTG TTGGACCACC ACCCATCCAG 
CCTGGATCCC CTGAATGGGT GTACGTAAAT GAGCCACTTC CGAAAGAAAT CCCAGCATTC 
CTTGTACCAT ATTGGAAGAC TATAGAAAAT GTCTATGGAA TGACCATCAA AACTGTGCTG 
AGGAATCTGA GGCAGGAACG ACTAACTGTG ATTCACTATT TATATGATAT CAGGGTTCAT 
TTGAAGGATT ACTTGAAGCG GCCGGATCAC AAGCAGGAAT TCGTGTCTCA GTGGCAGTCG 
GACTTTAATT CTACCGCAGA CGACTTGTGG GAGGACGAGG AAACAAAAGC AGAGCTTCAT 
CAAAGAGCTG ATGATCTCCG GGACCGTCTG TGGGACATAT GTGACAACAG GAAGGAGGAG 
GCAGAACAAG AGCGCACAGA CATTATGAAT GATGGGTGGC TACAGGATCA CACCGGGATA 
CTTATTAACC ACTTTTTCTC TCTGATGCAG GCGGAAGTTG ATCGCTTCCA AGACACCACA 
AGACTTCTCA GTGATTACTA CCAAGGAATG GAGGGACAAG CCCCATCTGA GGCCAGCCAT 
ACCTTTTCCC GTATCCCACT GCTGGACATT GCTAACTCAA GCACGCAGGG GGATTCTGAG 
AACCCTAAAA GAATTCCTCT TATACCCCGA AGAGCCCAGT CACCTGAACA GAACACAGGC 
AAACAGAAAG CCAAAAGTCA AGCCAAGGCA AAGGAGGAGC CCCCCACAGA CCACCTTGGG 
CAGATCTCCG AGGCTGATGA AAAACTTATC ACTGACACCT GGCAAACTGC TGTTTCAGCC 
ATTGCAAACA TGGTGTCGAA TGAAAAACAG TCCAAAGAAG CAGAAGAGGA AAAAGAAAGG 
CAACTGATGG AAATGAAGGA AAAAGAGAGA GCGAAAGCTT CACAAGCTGC ATCTCGGGCT 
AGTGGCAAAG GAGGGAAAAA GAAAGCGCCG CCAAAGTCGC CAAACAGGAA AAAAGAAGCA 
AAGTCCCCAG GCCCTGCAGA CCCCACACCT TCTCTTCAGA CCCTAGAAGA CAGTACTGAG 
TTGCAAAAGA AGCAGGAGCT AAAGATGAAG TTGAAGCAGG AGCATATTGC TGCTTTAGAG 
TATGAAGAGA TGGCTGCTAA CTTACGGCTG GAAGTTATTA AAACAAAAGC CTTAGAAGTG 
TGCCAGGATA TTGCATCGAG AGCTGAGAGT GCCTACAAAG ATATGGAAAT GTGGATGGGA 
GCAAGATTCC TGGCAGAAAT GGCAAGCGTG GAGCAACTCA TCCATATTGC TCGTCACCAT 
ATTGAAACGG GCACCAAAAT AAAGTATCAG CTTGTCCTGG AGAATACAGA TTTCTACATT 
AATTCAGATG TACAAGTGGT GCCCGATCCT CCGCCACCTT CTCGCCCTCC TGCAGTAGAG 
ATATCAGCCA ATGCGACACT GACTGTCGCT CAGCTCCACA GATTATACCA GCAGTTTGCT 
CAGATCGCTC CTGAAGGACT AATATCCAAT AAAACGTTTA TCGATATTCT CCTGGATATT 
ACATCAGCGA GACTTGGAAA CGATGCCCTC CCTGATTCGT GGATGCAGCT TACACTTCCA 
GAGATAAAAG AAATGACATC TGAATTATCT CAGAACTCTG ATGTGGTAAA CTGGCGCCTA 
TTCCTGCTGT CAGCATCTCT GCCATGGCCG TACCCCTCCG TAACGCAGCT TCTCAGCACT 
CTGCAGAGCT TCAAGGCTGT GGATCATAAT GGCTGCGGCA CACTGACCCA CGAAGGTTTC 
AGTCAGGCTG AGCTGTGGTT TCATGGAAAT GCAGAGGAAC CTGTCCCTGA AAATCCCACA 
GAGCCTTTGC CATTTAACAG ACCGGCCCAT CTAATAAAGT ATTTCTTTGA CTTGTTTGCT 
GACCCCCGGG GAATCCCCCC CCAGCTGAAT TACACAGAGA TGCTGCTGTA TTTTGCCTCA 
CACCCCGACC CTATGGAAGG CTTCTATCGG GCGCTGAGTG TTACGGCGGG GAAGCCTATT 
CTGAAGGCAC CAGGAAATAA TGTTTTGCTT GAGTCTCTCC CAGCAATGGA GGCCATTCAG 
CAGATGAAAG AACCAATCAG CAGCAATGAC ACAGTGGGGA CACGAGGCTT GGGAAAGGCA 
GCGGTCACAT TACATGAAGT GGTGAGAGTG CTGCAGCACG CTGCTGGGAC GGGTGAAGAT 
AACCATAGGT TTTGCCTGGA AGAAAATGGC AGGAACAATA TTTATAAGGT ACTTTTACCT 
TTGTCTCATG AAAACAAGAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_031894698.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750558.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.

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CloneID	OXa49220
Clone ID Related Accession (Same CDS sequence)	XM_031894698.1
Accession Version	XM_031894698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5427bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA 
GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT 
ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA 
AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG 
GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT 
ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT 
ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC 
CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA 
TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT 
ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG 
CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG 
GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG 
CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTA 
ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC 
CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC 
TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG 
CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG 
GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA 
ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA 
GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT 
GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT 
AAGAGCCTCG GGCAGATATT TGCGGAGCTG GGCACCGAGC AGCAGGAATG TGTAGCTGTC 
GCAGCTCTTA TGAGCCACCC TGCATTCCAA GACCTTCTCA GCAACTGTCA CATCTACAAA 
TTCCCAGATA TTCATAGAAT TCTAAACAAG CTGAAAGCCC CCCAGGCGAG TGATGGAGAG 
ACTTTGCTGC CTGTGAGTTC AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750558.1
CDS	129..5555
Translation	MSDIICQWLNEDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKVPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTDLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKRSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKEAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKSLGQIFAELGTEQQECVAVAALMSHPAFQDLLSNCHIYKFPDIHRILNKLKAPQASDGETLLPVSSR

Target ORF information:

RefSeq Version	XM_031894698.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894698.1

ORF Insert Sequence:

ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG AAGCAAAGTC CCCAGGCCCT GCAGACCCCA CACCTTCTCT TCAGACCCTA 
GAAGACAGTA CTGAGTTGCA AAAGAAGCAG GAGCTAAAGA TGAAGTTGAA GCAGGAGCAT 
ATTGCTGCTT TAGAGTATGA AGAGATGGCT GCTAACTTAC GGCTGGAAGT TATTAAAACA 
AAAGCCTTAG AAGTGTGCCA GGATATTGCA TCGAGAGCTG AGAGTGCCTA CAAAGATATG 
GAAATGTGGA TGGGAGCAAG ATTCCTGGCA GAAATGGCAA GCGTGGAGCA ACTCATCCAT 
ATTGCTCGTC ACCATATTGA AACGGGCACC AAAATAAAGT ATCAGCTTGT CCTGGAGAAT 
ACAGATTTCT ACATTAATTC AGATGTACAA GTGGTGCCCG ATCCTCCGCC ACCTTCTCGC 
CCTCCTGCAG TAGAGATATC AGCCAATGCG ACACTGACTG TCGCTCAGCT CCACAGATTA 
TACCAGCAGT TTGCTCAGAT CGCTCCTGAA GGACTAATAT CCAATAAAAC GTTTATCGAT 
ATTCTCCTGG ATATTACATC AGCGAGACTT GGAAACGATG CCCTCCCTGA TTCGTGGATG 
CAGCTTACAC TTCCAGAGAT AAAAGAAATG ACATCTGAAT TATCTCAGAA CTCTGATGTG 
GTAAACTGGC GCCTATTCCT GCTGTCAGCA TCTCTGCCAT GGCCGTACCC CTCCGTAACG 
CAGCTTCTCA GCACTCTGCA GAGCTTCAAG GCTGTGGATC ATAATGGCTG CGGCACACTA 
ACCCACGAAG GTTTCAGTCA GGCTGAGCTG TGGTTTCATG GAAATGCAGA GGAACCTGTC 
CCTGAAAATC CCACAGAGCC TTTGCCATTT AACAGACCGG CCCATCTAAT AAAGTATTTC 
TTTGACTTGT TTGCTGACCC CCGGGGAATC CCCCCCCAGC TGAATTACAC AGAGATGCTG 
CTGTATTTTG CCTCACACCC CGACCCTATG GAAGGCTTCT ATCGGGCGCT GAGTGTTACG 
GCGGGGAAGC CTATTCTGAA GGCACCAGGA AATAATGTTT TGCTTGAGTC TCTCCCAGCA 
ATGGAGGCCA TTCAGCAGAT GAAAGAACCA ATCAGCAGCA ATGACACAGT GGGGACACGA 
GGCTTGGGAA AGGCAGCGGT CACATTACAT GAAGTGGTGA GAGTGCTGCA GCACGCTGCT 
GGGACGGGTG AAGATAACCA TAGGTTTTGC CTGGAAGAAA ATGGCAGGAA CAATATTTAT 
AAGAGCCTCG GGCAGATATT TGCGGAGCTG GGCACCGAGC AGCAGGAATG TGTAGCTGTC 
GCAGCTCTTA TGAGCCACCC TGCATTCCAA GACCTTCTCA GCAACTGTCA CATCTACAAA 
TTCCCAGATA TTCATAGAAT TCTAAACAAG CTGAAAGCCC CCCAGGCGAG TGATGGAGAG 
ACTTTGCTGC CTGTGAGTTC AAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_031894702.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750562.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.

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CloneID	OXa49221
Clone ID Related Accession (Same CDS sequence)	XM_031894702.1
Accession Version	XM_031894702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT 
GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA 
TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA 
AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG 
AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT 
AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA 
AAGGCTCTCA CTGGCTCACA CACTGATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT 
GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT 
GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA 
ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG 
AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC 
CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT 
GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC 
GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA 
GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT 
TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA 
TCTCCAAGAG AATCTCGAAA ACGGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA 
GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA 
AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA 
CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA 
ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC 
AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT 
ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT 
CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA 
GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC 
AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT 
CACACCGGGA TACTTATTAA CCACTTTTTC TCACTGATGC AGGCGGAAGT TGATCGCTTC 
CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT 
GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG 
GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA 
CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA 
GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT 
GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG 
GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT 
GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG 
AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA 
GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC 
CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT 
TTGCTGCCTG TGAGTTCAAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750562.1
CDS	129..5552
Translation	MSDIICQWLNEDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTDLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKRSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKEAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKSLGQIFAELGTEQQECVAVAALMSHPAFQDLLSNCHIYKFPDIHRILNKLKAPQASDGETLLPVSSR

Target ORF information:

RefSeq Version	XM_031894702.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894702.1

ORF Insert Sequence:

ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG CCCATAGACA GTCCTGCTCC TGTTACTAAT 
GGCGAAGAGA TACCTGTTGT GGGAAGTGGC AGCAAGCCTG CATCTGCAGA AGCCCGTGAA 
TCAGATAAAG AGTTATCTCT ACATCAAATA TTAAAGCCTG CAATAGAAGA TACTGTGCCA 
AAGCTCTCTG CACGGGCACA GCTTGGTGGA CTGGCAGAGA CACATTTAAA GAGTGGGAAG 
AGTGTCCCAG ATGAGCTGCT TATCAGTATA CTTGTTGAAG CCATCAATCG CATCCCAGCT 
AACACAGGGT GGATCATGGA GGGATTCCCT ATGACGGAAA CTCAGGCCAC ATTATTTGAA 
AAGGCTCTCA CTGGCTCACA CACTGATTTG AATGGAATAA AGGGGAATAA TAACAAGTTT 
GCCTCTTTAG TAACAGATCC AGCAGCACCA AAGGATCTCC CTGTGGCTCC ATCTGCTCTT 
GACTTTGCTG TGTTAATAGA AATATCAGAC AATGAAGTGT TACAGCGCAT GTCTACAATA 
ATCAGTGAAA TAGATAAATC TGGAGAGAAA ATTGATAGTC CTCCCTGTAT TCAAACTACG 
AGAATGGAAG CAGATCAAAC AATGGACCAG ATACAGCACA GAATTACAGG ATTTCTTGAC 
CACTGGAAGA ATTTAGAAAT GTGGTTTTCA GAGCAGCAGA ATATTCTAAT CAAAGTTAAT 
GGGGAAGTGG AAGTTGGCGT TCTGTGCAAG AGAATAGAAG AAGTATACTT GACAGCTCTC 
GTCAATAAGC AAAACCGAGG CAAAAAAAAC GAAAAGAAAG AAGATCTCCC ACCACCAGAA 
GTAGCCGCAC CAACACCGCC ACCGCCATCT CCTCCTCCAG CTCCAGAAGC CACTAACAAT 
TTACGACCTC CATCAGCTTC AAGCAAGGAA TCAGCAAAAA AAAGCAGGAA AGGCTCCAAA 
TCTCCAAGAG AATCTCGAAA ACGGTCTGAC ACCTCGAAGG ACAAGAAGGA CAAGAAACCA 
GAGACACCTA AAGGAAAAGA CTCTCGAAAA TCTGGTTCAC CACGTGGAAG GTCTCCTGGA 
AAGAAGGCTA AGTCTGTACC TGCCAGCCCT GAACCAGTCG CTGTCACTCC TGTTGGACCA 
CCACCCATCC AGCCTGGATC CCCTGAATGG GTGTACGTAA ATGAGCCACT TCCGAAAGAA 
ATCCCAGCAT TCCTTGTACC ATATTGGAAG ACTATAGAAA ATGTCTATGG AATGACCATC 
AAAACTGTGC TGAGGAATCT GAGGCAGGAA CGACTAACTG TGATTCACTA TTTATATGAT 
ATCAGGGTTC ATTTGAAGGA TTACTTGAAG CGGCCGGATC ACAAGCAGGA ATTCGTGTCT 
CAGTGGCAGT CGGACTTTAA TTCTACCGCA GACGACTTGT GGGAGGACGA GGAAACAAAA 
GCAGAGCTTC ATCAAAGAGC TGATGATCTC CGGGACCGTC TGTGGGACAT ATGTGACAAC 
AGGAAGGAGG AGGCAGAACA AGAGCGCACA GACATTATGA ATGATGGGTG GCTACAGGAT 
CACACCGGGA TACTTATTAA CCACTTTTTC TCACTGATGC AGGCGGAAGT TGATCGCTTC 
CAAGACACCA CAAGACTTCT CAGTGATTAC TACCAAGGAA TGGAGGGACA AGCCCCATCT 
GAGGCCAGCC ATACCTTTTC CCGTATCCCA CTGCTGGACA TTGCTAACTC AAGCACGCAG 
GGGGATTCTG AGAACCCTAA AAGAATTCCT CTTATACCCC GAAGAGCCCA GTCACCTGAA 
CAGAACACAG GCAAACAGAA AGCCAAAAGT CAAGCCAAGG CAAAGGAGGA GCCCCCCACA 
GACCACCTTG GGCAGATCTC CGAGGCTGAT GAAAAACTTA TCACTGACAC CTGGCAAACT 
GCTGTTTCAG CCATTGCAAA CATGGTGTCG AATGAAAAAC AGTCCAAAGA AGCAGAAGAG 
GAAAAAGAAA GGCAACTGAT GGAAATGAAG GAAAAAGAGA GAGCGAAAGC TTCACAAGCT 
GCATCTCGGG CTAGTGGCAA AGGAGGGAAA AAGAAAGCGC CGCCAAAGTC GCCAAACAGG 
AAAAAAGAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA 
GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC 
CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT 
TTGCTGCCTG TGAGTTCAAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spef2 ( XM_031894707.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031750567.1 Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.

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CloneID	OXa49222
Clone ID Related Accession (Same CDS sequence)	XM_031894707.1
Accession Version	XM_031894707.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA 
GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC 
CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT 
TTGCTGCCTG TGAGTTCAAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031750567.1
CDS	129..5552
Translation	MSDIICQWLNEDLKLSKRVEPKYLAKEFSSGYLIGEVLNKFQLQEDFDQFSQTRVANGKLNNFTRIEPTLQLLGVPFDQNVAHSIMSEQHGVATRLLYQLFIALQKKKKAGLTGVAMETLRPAAPAKLQSIGTEMYRERLKTIIPRQSEISLQRVSEQFDLKAKEIEDKVAQIQNDELRKIQKIQEELRIQDIEKLRRARRRQTEIMARIQAAIVQIPKPPQNRTLKAIEAQKMLKKKKEAEEVYTEITKFEKGVKDGSVGSLSANTRGPTLQRLRSSVNIGTALLKLDTNDDYVRKIQKRLEEDSVAREQREKRRRRILMEQLVGHGAQEEAFREEQLINRLMRQSQQERRIAVQLMHARHEKEVMQQNRMFREKQYQERREREFQEALDREAALQKQAMLDHKEEIRKVQELHDQIAAERAEARYKKHYNLCQEIMNQILDFVIKTGEYRELTNRLIPVKLMREWKELFFAGYPLYDKAPIDALPSDPTAEQLQETEKQSLLDEKDYEEYKTMTGEWGPPEASSVKAPAPNNNILGYVVHRLLEIVSPPLAPTPPPVFPPFPIKGCVLGKIYSGKSTCLKHLAQVYSIQVLSVDTLLEEAIQAFNDGELEKTEDVLRPIQEGADKENKVPIDSPAPVTNGEEIPVVGSGSKPASAEARESDKELSLHQILKPAIEDTVPKLSARAQLGGLAETHLKSGKSVPDELLISILVEAINRIPANTGWIMEGFPMTETQATLFEKALTGSHTDLNGIKGNNNKFASLVTDPAAPKDLPVAPSALDFAVLIEISDNEVLQRMSTIISEIDKSGEKIDSPPCIQTTRMEADQTMDQIQHRITGFLDHWKNLEMWFSEQQNILIKVNGEVEVGVLCKRIEEVYLTALVNKQNRGKKNEKKEDLPPPEVAAPTPPPPSPPPAPEATNNLRPPSASSKESAKKSRKGSKSPRESRKRSDTSKDKKDKKPETPKGKDSRKSGSPRGRSPGKKAKSVPASPEPVAVTPVGPPPIQPGSPEWVYVNEPLPKEIPAFLVPYWKTIENVYGMTIKTVLRNLRQERLTVIHYLYDIRVHLKDYLKRPDHKQEFVSQWQSDFNSTADDLWEDEETKAELHQRADDLRDRLWDICDNRKEEAEQERTDIMNDGWLQDHTGILINHFFSLMQAEVDRFQDTTRLLSDYYQGMEGQAPSEASHTFSRIPLLDIANSSTQGDSENPKRIPLIPRRAQSPEQNTGKQKAKSQAKAKEEPPTDHLGQISEADEKLITDTWQTAVSAIANMVSNEKQSKEAEEEKERQLMEMKEKERAKASQAASRASGKGGKKKAPPKSPNRKKAKSPGPADPTPSLQTLEDSTELQKKQELKMKLKQEHIAALEYEEMAANLRLEVIKTKALEVCQDIASRAESAYKDMEMWMGARFLAEMASVEQLIHIARHHIETGTKIKYQLVLENTDFYINSDVQVVPDPPPPSRPPAVEISANATLTVAQLHRLYQQFAQIAPEGLISNKTFIDILLDITSARLGNDALPDSWMQLTLPEIKEMTSELSQNSDVVNWRLFLLSASLPWPYPSVTQLLSTLQSFKAVDHNGCGTLTHEGFSQAELWFHGNAEEPVPENPTEPLPFNRPAHLIKYFFDLFADPRGIPPQLNYTEMLLYFASHPDPMEGFYRALSVTAGKPILKAPGNNVLLESLPAMEAIQQMKEPISSNDTVGTRGLGKAAVTLHEVVRVLQHAAGTGEDNHRFCLEENGRNNIYKSLGQIFAELGTEQQECVAVAALMSHPAFQDLLSNCHIYKFPDIHRILNKLKAPQASDGETLLPVSSR

Target ORF information:

RefSeq Version	XM_031894707.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis sperm flagellar 2 (spef2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031894707.1

ORF Insert Sequence:

ATGTCGGACA TTATCTGCCA GTGGCTTAAT GAGGACCTCA AACTCTCCAA GCGTGTGGAA 
CCAAAGTATT TAGCAAAGGA GTTTTCCAGT GGATATCTCA TTGGGGAAGT TCTGAACAAA 
TTTCAGCTAC AAGAGGACTT TGATCAGTTT TCTCAAACTA GAGTGGCCAA CGGCAAGCTA 
AATAACTTTA CCCGCATTGA ACCAACGCTC CAGCTTTTGG GTGTTCCTTT TGACCAGAAT 
GTGGCTCATA GTATTATGTC AGAACAGCAC GGAGTGGCTA CCCGGCTTCT GTATCAGTTG 
TTCATAGCAT TGCAGAAGAA AAAGAAGGCA GGATTGACAG GAGTAGCCAT GGAGACATTG 
AGACCAGCTG CTCCAGCAAA GCTTCAGAGC ATTGGAACTG AGATGTACAG AGAGCGTTTG 
AAAACCATAA TCCCCCGCCA AAGTGAAATC AGCCTGCAGA GGGTGTCAGA ACAATTTGAC 
CTGAAAGCAA AAGAAATCGA AGACAAAGTT GCACAGATTC AAAATGATGA ACTTCGGAAA 
ATACAGAAAA TACAAGAAGA GCTAAGAATT CAGGATATTG AAAAGCTGCG ACGAGCTAGA 
AGGAGACAAA CCGAAATAAT GGCACGAATT CAAGCAGCAA TTGTTCAGAT TCCAAAACCA 
CCTCAAAACC GCACATTGAA GGCCATCGAA GCACAAAAGA TGCTGAAAAA GAAGAAAGAA 
GCTGAGGAAG TTTATACAGA AATCACAAAG TTTGAAAAAG GAGTGAAAGA TGGATCTGTT 
GGAAGCTTGT CTGCTAATAC CAGGGGCCCA ACACTCCAGA GATTAAGGTC ATCAGTTAAC 
ATTGGCACTG CTTTGCTAAA ACTGGATACT AATGATGATT ACGTAAGGAA AATTCAGAAG 
CGATTAGAGG AGGATTCTGT AGCACGTGAG CAGCGCGAAA AGAGACGTCG GCGGATTCTT 
ATGGAACAGC TTGTGGGTCA TGGGGCACAA GAGGAAGCTT TCCGGGAGGA ACAACTGATA 
AACCGATTAA TGAGACAGTC CCAGCAAGAG CGGAGAATTG CAGTTCAGCT TATGCACGCG 
CGCCATGAAA AGGAAGTTAT GCAACAGAAC CGAATGTTCC GGGAGAAGCA ATATCAGGAG 
AGGCGCGAGA GGGAGTTCCA AGAAGCTCTT GACAGAGAAG CGGCGTTGCA GAAACAAGCG 
ATGCTCGACC ACAAAGAAGA AATACGCAAA GTGCAGGAGC TCCATGACCA GATAGCAGCG 
GAGCGTGCAG AGGCTCGCTA TAAAAAGCAC TATAACCTGT GCCAGGAAAT CATGAATCAG 
ATTTTAGACT TTGTTATTAA AACAGGGGAA TACAGAGAGC TAACAAACAG ACTTATACCT 
GTTAAATTGA TGCGGGAGTG GAAAGAACTA TTCTTCGCTG GTTACCCGCT TTACGACAAA 
GCACCTATAG ATGCCCTCCC TTCCGACCCA ACTGCAGAGC AGCTGCAAGA AACAGAAAAG 
CAGAGTCTGT TAGATGAGAA GGATTATGAA GAATACAAGA CCATGACAGG GGAATGGGGC 
CCACCCGAAG CCAGCAGTGT TAAAGCACCT GCACCGAACA ACAACATATT GGGCTATGTG 
GTTCATAGAC TGTTGGAAAT TGTCAGCCCC CCATTGGCAC CCACACCGCC ACCTGTGTTT 
CCCCCCTTCC CCATCAAGGG TTGTGTTCTT GGGAAGATTT ACTCTGGGAA GAGCACGTGC 
CTGAAACATC TGGCACAGGT GTATAGCATC CAAGTTCTCT CTGTGGACAC CCTTCTGGAG 
GAAGCTATTC AAGCATTTAA TGACGGGGAA CTGGAAAAGA CCGAGGATGT TCTGAGGCCC 
ATACAAGAAG GAGCAGATAA AGAGAACAAG GTACCCATAG ACAGTCCTGC TCCTGTTACT 
AATGGCGAAG AGATACCTGT TGTGGGAAGT GGCAGCAAGC CTGCATCTGC AGAAGCCCGT 
GAATCAGATA AAGAGTTATC TCTACATCAA ATATTAAAGC CTGCAATAGA AGATACTGTG 
CCAAAGCTCT CTGCACGGGC ACAGCTTGGT GGACTGGCAG AGACACATTT AAAGAGTGGG 
AAGAGTGTCC CAGATGAGCT GCTTATCAGT ATACTTGTTG AAGCCATCAA TCGCATCCCA 
GCTAACACAG GGTGGATCAT GGAGGGATTC CCTATGACGG AAACTCAGGC CACATTATTT 
GAAAAGGCTC TCACTGGCTC ACACACTGAT TTGAATGGAA TAAAGGGGAA TAATAACAAG 
TTTGCCTCTT TAGTAACAGA TCCAGCAGCA CCAAAGGATC TCCCTGTGGC TCCATCTGCT 
CTTGACTTTG CTGTGTTAAT AGAAATATCA GACAATGAAG TGTTACAGCG CATGTCTACA 
ATAATCAGTG AAATAGATAA ATCTGGAGAG AAAATTGATA GTCCTCCCTG TATTCAAACT 
ACGAGAATGG AAGCAGATCA AACAATGGAC CAGATACAGC ACAGAATTAC AGGATTTCTT 
GACCACTGGA AGAATTTAGA AATGTGGTTT TCAGAGCAGC AGAATATTCT AATCAAAGTT 
AATGGGGAAG TGGAAGTTGG CGTTCTGTGC AAGAGAATAG AAGAAGTATA CTTGACAGCT 
CTCGTCAATA AGCAAAACCG AGGCAAAAAA AACGAAAAGA AAGAAGATCT CCCACCACCA 
GAAGTAGCCG CACCAACACC GCCACCGCCA TCTCCTCCTC CAGCTCCAGA AGCCACTAAC 
AATTTACGAC CTCCATCAGC TTCAAGCAAG GAATCAGCAA AAAAAAGCAG GAAAGGCTCC 
AAATCTCCAA GAGAATCTCG AAAACGGTCT GACACCTCGA AGGACAAGAA GGACAAGAAA 
CCAGAGACAC CTAAAGGAAA AGACTCTCGA AAATCTGGTT CACCACGTGG AAGGTCTCCT 
GGAAAGAAGG CTAAGTCTGT ACCTGCCAGC CCTGAACCAG TCGCTGTCAC TCCTGTTGGA 
CCACCACCCA TCCAGCCTGG ATCCCCTGAA TGGGTGTACG TAAATGAGCC ACTTCCGAAA 
GAAATCCCAG CATTCCTTGT ACCATATTGG AAGACTATAG AAAATGTCTA TGGAATGACC 
ATCAAAACTG TGCTGAGGAA TCTGAGGCAG GAACGACTAA CTGTGATTCA CTATTTATAT 
GATATCAGGG TTCATTTGAA GGATTACTTG AAGCGGCCGG ATCACAAGCA GGAATTCGTG 
TCTCAGTGGC AGTCGGACTT TAATTCTACC GCAGACGACT TGTGGGAGGA CGAGGAAACA 
AAAGCAGAGC TTCATCAAAG AGCTGATGAT CTCCGGGACC GTCTGTGGGA CATATGTGAC 
AACAGGAAGG AGGAGGCAGA ACAAGAGCGC ACAGACATTA TGAATGATGG GTGGCTACAG 
GATCACACCG GGATACTTAT TAACCACTTT TTCTCACTGA TGCAGGCGGA AGTTGATCGC 
TTCCAAGACA CCACAAGACT TCTCAGTGAT TACTACCAAG GAATGGAGGG ACAAGCCCCA 
TCTGAGGCCA GCCATACCTT TTCCCGTATC CCACTGCTGG ACATTGCTAA CTCAAGCACG 
CAGGGGGATT CTGAGAACCC TAAAAGAATT CCTCTTATAC CCCGAAGAGC CCAGTCACCT 
GAACAGAACA CAGGCAAACA GAAAGCCAAA AGTCAAGCCA AGGCAAAGGA GGAGCCCCCC 
ACAGACCACC TTGGGCAGAT CTCCGAGGCT GATGAAAAAC TTATCACTGA CACCTGGCAA 
ACTGCTGTTT CAGCCATTGC AAACATGGTG TCGAATGAAA AACAGTCCAA AGAAGCAGAA 
GAGGAAAAAG AAAGGCAACT GATGGAAATG AAGGAAAAAG AGAGAGCGAA AGCTTCACAA 
GCTGCATCTC GGGCTAGTGG CAAAGGAGGG AAAAAGAAAG CGCCGCCAAA GTCGCCAAAC 
AGGAAAAAAG CAAAGTCCCC AGGCCCTGCA GACCCCACAC CTTCTCTTCA GACCCTAGAA 
GACAGTACTG AGTTGCAAAA GAAGCAGGAG CTAAAGATGA AGTTGAAGCA GGAGCATATT 
GCTGCTTTAG AGTATGAAGA GATGGCTGCT AACTTACGGC TGGAAGTTAT TAAAACAAAA 
GCCTTAGAAG TGTGCCAGGA TATTGCATCG AGAGCTGAGA GTGCCTACAA AGATATGGAA 
ATGTGGATGG GAGCAAGATT CCTGGCAGAA ATGGCAAGCG TGGAGCAACT CATCCATATT 
GCTCGTCACC ATATTGAAAC GGGCACCAAA ATAAAGTATC AGCTTGTCCT GGAGAATACA 
GATTTCTACA TTAATTCAGA TGTACAAGTG GTGCCCGATC CTCCGCCACC TTCTCGCCCT 
CCTGCAGTAG AGATATCAGC CAATGCGACA CTGACTGTCG CTCAGCTCCA CAGATTATAC 
CAGCAGTTTG CTCAGATCGC TCCTGAAGGA CTAATATCCA ATAAAACGTT TATCGATATT 
CTCCTGGATA TTACATCAGC GAGACTTGGA AACGATGCCC TCCCTGATTC GTGGATGCAG 
CTTACACTTC CAGAGATAAA AGAAATGACA TCTGAATTAT CTCAGAACTC TGATGTGGTA 
AACTGGCGCC TATTCCTGCT GTCAGCATCT CTGCCATGGC CGTACCCCTC CGTAACGCAG 
CTTCTCAGCA CTCTGCAGAG CTTCAAGGCT GTGGATCATA ATGGCTGCGG CACACTAACC 
CACGAAGGTT TCAGTCAGGC TGAGCTGTGG TTTCATGGAA ATGCAGAGGA ACCTGTCCCT 
GAAAATCCCA CAGAGCCTTT GCCATTTAAC AGACCGGCCC ATCTAATAAA GTATTTCTTT 
GACTTGTTTG CTGACCCCCG GGGAATCCCC CCCCAGCTGA ATTACACAGA GATGCTGCTG 
TATTTTGCCT CACACCCCGA CCCTATGGAA GGCTTCTATC GGGCGCTGAG TGTTACGGCG 
GGGAAGCCTA TTCTGAAGGC ACCAGGAAAT AATGTTTTGC TTGAGTCTCT CCCAGCAATG 
GAGGCCATTC AGCAGATGAA AGAACCAATC AGCAGCAATG ACACAGTGGG GACACGAGGC 
TTGGGAAAGG CAGCGGTCAC ATTACATGAA GTGGTGAGAG TGCTGCAGCA CGCTGCTGGG 
ACGGGTGAAG ATAACCATAG GTTTTGCCTG GAAGAAAATG GCAGGAACAA TATTTATAAG 
AGCCTCGGGC AGATATTTGC GGAGCTGGGC ACCGAGCAGC AGGAATGTGT AGCTGTCGCA 
GCTCTTATGA GCCACCCTGC ATTCCAAGAC CTTCTCAGCA ACTGTCACAT CTACAAATTC 
CCAGATATTC ATAGAATTCT AAACAAGCTG AAAGCCCCCC AGGCGAGTGA TGGAGAGACT 
TTGCTGCCTG TGAGTTCAAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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spef2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

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