SPEF2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005248376.3	XP_005248433.1	sperm flagellar protein 2 isoform X9
XM_011514135.2	XP_011512437.1	sperm flagellar protein 2 isoform X1
NM_024867.3	NP_079143.3	sperm flagellar protein 2 isoform 1
XM_011514140.2	XP_011512442.1	sperm flagellar protein 2 isoform X6
XM_011514141.3	XP_011512443.1	sperm flagellar protein 2 isoform X8
XM_005248376.4	XP_005248433.1	sperm flagellar protein 2 isoform X9
XM_017009882.1	XP_016865371.1	sperm flagellar protein 2 isoform X12
XM_011514136.2	XP_011512438.1	sperm flagellar protein 2 isoform X2
XM_011514139.2	XP_011512441.1	sperm flagellar protein 2 isoform X5
NM_144722.4	NP_653323.1	sperm flagellar protein 2 isoform 2
XM_011514135.3	XP_011512437.1	sperm flagellar protein 2 isoform X1
XM_011514139.3	XP_011512441.1	sperm flagellar protein 2 isoform X5
XM_017009880.2	XP_016865369.1	sperm flagellar protein 2 isoform X7
XM_005248377.4	XP_005248434.1	sperm flagellar protein 2 isoform X10
XM_024446219.1	XP_024301987.1	sperm flagellar protein 2 isoform X13
XM_011514137.2	XP_011512439.1	sperm flagellar protein 2 isoform X3
XM_017009881.1	XP_016865370.1	sperm flagellar protein 2 isoform X8
XM_005248378.3	XP_005248435.1	sperm flagellar protein 2 isoform X13
NM_144722.3	NP_653323.1	sperm flagellar protein 2 isoform 2
XM_011514136.3	XP_011512438.1	sperm flagellar protein 2 isoform X2
XM_011514137.3	XP_011512439.1	sperm flagellar protein 2 isoform X3
XM_011514138.2	XP_011512440.1	sperm flagellar protein 2 isoform X4
XM_017009880.1	XP_016865369.1	sperm flagellar protein 2 isoform X7
NM_024867.4	NP_079143.3	sperm flagellar protein 2 isoform 1
XM_011514141.2	XP_011512443.1	sperm flagellar protein 2 isoform X8
XM_005248377.3	XP_005248434.1	sperm flagellar protein 2 isoform X10
XM_011514142.1	XP_011512444.1	sperm flagellar protein 2 isoform X11
XM_011514138.3	XP_011512440.1	sperm flagellar protein 2 isoform X4
XM_017009881.2	XP_016865370.1	sperm flagellar protein 2 isoform X8
XM_017009882.2	XP_016865371.1	sperm flagellar protein 2 isoform X11
XM_005248378.4	XP_005248435.1	sperm flagellar protein 2 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02181	NM_024867.4 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu02105	NM_144722.4 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu39379	XM_005248378.4 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu96094	XM_017009882.2 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu105700	XM_024446219.1 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu39375	XM_005248376.4 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu39376	XM_005248377.4 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72409	XM_011514141.3 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72409	XM_017009881.2 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72410	XM_011514142.1 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu96093	XM_017009880.2 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu72403	XM_011514135.3 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72404	XM_011514136.3 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72405	XM_011514137.3 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72406	XM_011514138.3 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72407	XM_011514139.3 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72408	XM_011514140.2 Latest version!	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu39375	XM_005248376.3	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72403	XM_011514135.2	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72407	XM_011514139.2	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu02105	NM_144722.3	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu72404	XM_011514136.2	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72405	XM_011514137.2	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu96093	XM_017009880.1	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu02181	NM_024867.3	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu72409	XM_011514141.2	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu39376	XM_005248377.3	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu72406	XM_011514138.2	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu72409	XM_017009881.1	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu96094	XM_017009882.1	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu39379	XM_005248378.3	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

SPEF2 ( NM_024867.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_079143.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02181
Clone ID Related Accession (Same CDS sequence)	NM_024867.3 , NM_024867.4
Accession Version	NM_024867.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC067761.1, BC016773.1, BC110984.1, AL832607.1, AK097547.1, AB051557.1, DA295300.1 and BX648691.1. On Apr 25, 2007 this sequence version replaced NM_024867.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079143.3
CDS	112..5580
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	NM_024867.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024867.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( NM_024867.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079143.3 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.

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CloneID	OHu02181
Clone ID Related Accession (Same CDS sequence)	NM_024867.3 , NM_024867.4
Accession Version	NM_024867.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC025448.6, BC067761.1, BC016773.1, BC110984.1, AL832607.1, AK097547.1, AB051557.1, DA295300.1 and BX648691.1. On Nov 22, 2018 this sequence version replaced NM_024867.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: HM005670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356031.8/ ENSP00000348314.3 RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079143.3
CDS	136..5604
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	NM_024867.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024867.4

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG ATATTAAAAT AATTCTCCAA 
AGGAGTGAAC ATGTACAAGG AAGTGATGGA GAGAGATCAC CTTCAAGACA TACAGAGGAA 
AAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( NM_144722.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_653323.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.

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CloneID	OHu02105
Clone ID Related Accession (Same CDS sequence)	NM_144722.4 , NM_144722.3
Accession Version	NM_144722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC025448.6, BC016773.1 and AK058124.1. On May 31, 2019 this sequence version replaced NM_144722.3. Transcript Variant: This variant (2) lacks more than half of the 3'-most exons and has a 3' end that extends into an intron compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.68671.1, BC067761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653323.1
CDS	136..1680
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKVPTDMK

Target ORF information:

RefSeq Version	NM_144722.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144722.4

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( NM_144722.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653323.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.

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CloneID	OHu02105
Clone ID Related Accession (Same CDS sequence)	NM_144722.4 , NM_144722.3
Accession Version	NM_144722.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC016773.1, AK058124.1 and AC025448.6. On Apr 25, 2006 this sequence version replaced NM_144722.2. Transcript Variant: This variant (2) lacks more than half of the 3'-most exons and has a 3' end that extends into an intron compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.68671.1, BC067761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653323.1
CDS	112..1656
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKVPTDMK

Target ORF information:

RefSeq Version	NM_144722.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144722.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGGTACCT ACTGATATGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_005248378.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248435.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

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CloneID	OHu39379
Clone ID Related Accession (Same CDS sequence)	XM_005248378.3 , XM_005248378.4
Accession Version	XM_005248378.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA 
GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248435.1
CDS	206..3124
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSGGDMESSHLGLLIRVRPQTRRGKGTE

Target ORF information:

RefSeq Version	XM_005248378.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248378.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA 
GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_005248378.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248435.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu39379
Clone ID Related Accession (Same CDS sequence)	XM_005248378.3 , XM_005248378.4
Accession Version	XM_005248378.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248378.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA 
GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248435.1
CDS	104..3022
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSGGDMESSHLGLLIRVRPQTRRGKGTE

Target ORF information:

RefSeq Version	XM_005248378.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248378.4

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG AGGAGACATG GAGTCATCAC ACCTAGGTCT ACTGATTAGA 
GTAAGACCAC AGACGAGAAG AGGAAAAGGC ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017009882.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865371.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu96094
Clone ID Related Accession (Same CDS sequence)	XM_017009882.1 , XM_017009882.2
Accession Version	XM_017009882.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT 
TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT 
GAAGGGCAAG ATGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865371.1
CDS	210..3767
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQNSSSSSNIHFSGNSYTQTKNKISTEGQDG

Target ORF information:

RefSeq Version	XM_017009882.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009882.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT 
TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT 
GAAGGGCAAG ATGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017009882.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865371.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.

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CloneID	OHu96094
Clone ID Related Accession (Same CDS sequence)	XM_017009882.1 , XM_017009882.2
Accession Version	XM_017009882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT 
TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT 
GAAGGGCAAG ATGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865371.1
CDS	104..3661
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQNSSSSSNIHFSGNSYTQTKNKISTEGQDG

Target ORF information:

RefSeq Version	XM_017009882.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009882.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGAA TTCCTCTAGT 
TCCTCGAATA TCCATTTCTC TGGAAACAGT TACACCCAAA CCAAAAACAA AATCAGTACT 
GAAGGGCAAG ATGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_024446219.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024301987.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu105700
Clone ID Related Accession (Same CDS sequence)	XM_024446219.1
Accession Version	XM_024446219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG 
CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGCATAATAG GAAATAAAGC ATTTACTGAC ATTCTGATCG ATTTGGTGAC CCTGAACCTT 
GGCACAAACA ACTTTCCTAG TAATTGGATG CACCTTACCC AACCTGAATT ACAGGAATTA 
ACATCTTTAT TAACAGTCAA CTCCGAGTTC GTGGACTGGC GGAAGTTCCT GTTAGTAACC 
TCAATGCCTT GGCCCATTCC CTTGGAGGAG GAGCTCCTTG AGACCCTTCA GAAGTTCAAG 
GCTGTGGATA AGGAGCAGTT GGGCACCATC ACTTTTGAGC AGTATATGCA GGCTGGTCTG 
TGGTTTACAG GAGATGAAGA TATAAAAATT CCAGAAAATC CTCTTGAACC CCTTCCATTT 
AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGCTAT TTGCTGACTA TGAGAAGGAT 
CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTACTTTG CTTGCCACCC AGACACCGTG 
GAAGGAGTCT ACAGGGCCCT CAGTGTGGCT GTTGGAACTC ATGTCTTCCA ACAAGTCAAA 
GCTTCCATTC CAAGTGCAGA AAAGACCTCC TCAACTGATG CAGGTCCAGC TGAGGAATTT 
CCTGAACCTG AGGAAAATGC TGCAAGAGAA GAAAGGAAAT TAAAAGACGA CACGGAGAAA 
AGGGAACAGA AGGATGAAGA AATCCCTGAA AATGCAAACA ATGAAAAGAT GTCCATGGAA 
ACACTACTCA AAGTGTTCAA AGGGGGAAGT GAAGCACAGG ACTCCAATAG ATTTGCCAGC 
CACCTAAAGA TAGAGAACAT TTATGCAGAG GGCTTCATAA AAACATTTCA AGACCTAGGT 
GCCAAGAACC TGGAGCCAAT TGAAGTCGCT GTTCTCTTGA AGCATCCTTT TATTCAAGAC 
CTGATTTCAA ATTATTCAGA CTATAAGTTT CCTGATATTA AAATAATTCT CCAAAGGAGT 
GAACATGTAC AAGGAAGTGA TGGAGAGAGA TCACCTTCAA GACATACAGA GGAAAAGAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024301987.1
CDS	632..5854
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQEQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_024446219.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446219.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG 
CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGCATAATAG GAAATAAAGC ATTTACTGAC ATTCTGATCG ATTTGGTGAC CCTGAACCTT 
GGCACAAACA ACTTTCCTAG TAATTGGATG CACCTTACCC AACCTGAATT ACAGGAATTA 
ACATCTTTAT TAACAGTCAA CTCCGAGTTC GTGGACTGGC GGAAGTTCCT GTTAGTAACC 
TCAATGCCTT GGCCCATTCC CTTGGAGGAG GAGCTCCTTG AGACCCTTCA GAAGTTCAAG 
GCTGTGGATA AGGAGCAGTT GGGCACCATC ACTTTTGAGC AGTATATGCA GGCTGGTCTG 
TGGTTTACAG GAGATGAAGA TATAAAAATT CCAGAAAATC CTCTTGAACC CCTTCCATTT 
AATAGGCAGG AGCATCTTAT AGAGTTTTTC TTTAGGCTAT TTGCTGACTA TGAGAAGGAT 
CCACCCCAGC TTGACTACAC ACAGATGCTG CTTTACTTTG CTTGCCACCC AGACACCGTG 
GAAGGAGTCT ACAGGGCCCT CAGTGTGGCT GTTGGAACTC ATGTCTTCCA ACAAGTCAAA 
GCTTCCATTC CAAGTGCAGA AAAGACCTCC TCAACTGATG CAGGTCCAGC TGAGGAATTT 
CCTGAACCTG AGGAAAATGC TGCAAGAGAA GAAAGGAAAT TAAAAGACGA CACGGAGAAA 
AGGGAACAGA AGGATGAAGA AATCCCTGAA AATGCAAACA ATGAAAAGAT GTCCATGGAA 
ACACTACTCA AAGTGTTCAA AGGGGGAAGT GAAGCACAGG ACTCCAATAG ATTTGCCAGC 
CACCTAAAGA TAGAGAACAT TTATGCAGAG GGCTTCATAA AAACATTTCA AGACCTAGGT 
GCCAAGAACC TGGAGCCAAT TGAAGTCGCT GTTCTCTTGA AGCATCCTTT TATTCAAGAC 
CTGATTTCAA ATTATTCAGA CTATAAGTTT CCTGATATTA AAATAATTCT CCAAAGGAGT 
GAACATGTAC AAGGAAGTGA TGGAGAGAGA TCACCTTCAA GACATACAGA GGAAAAGAAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_005248376.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248433.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu39375
Clone ID Related Accession (Same CDS sequence)	XM_005248376.3 , XM_005248376.4
Accession Version	XM_005248376.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005248376.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248433.1
CDS	217..5688
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_005248376.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248376.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_005248376.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248433.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

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CloneID	OHu39375
Clone ID Related Accession (Same CDS sequence)	XM_005248376.3 , XM_005248376.4
Accession Version	XM_005248376.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248376.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248433.1
CDS	104..5575
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_005248376.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248376.4

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGCA TAATAGGAAA TAAAGCATTT ACTGACATTC TGATCGATTT GGTGACCCTG 
AACCTTGGCA CAAACAACTT TCCTAGTAAT TGGATGCACC TTACCCAACC TGAATTACAG 
GAATTAACAT CTTTATTAAC AGTCAACTCC GAGTTCGTGG ACTGGCGGAA GTTCCTGTTA 
GTAACCTCAA TGCCTTGGCC CATTCCCTTG GAGGAGGAGC TCCTTGAGAC CCTTCAGAAG 
TTCAAGGCTG TGGATAAGGA GCAGTTGGGC ACCATCACTT TTGAGCAGTA TATGCAGGCT 
GGTCTGTGGT TTACAGGAGA TGAAGATATA AAAATTCCAG AAAATCCTCT TGAACCCCTT 
CCATTTAATA GGCAGGAGCA TCTTATAGAG TTTTTCTTTA GGCTATTTGC TGACTATGAG 
AAGGATCCAC CCCAGCTTGA CTACACACAG ATGCTGCTTT ACTTTGCTTG CCACCCAGAC 
ACCGTGGAAG GAGTCTACAG GGCCCTCAGT GTGGCTGTTG GAACTCATGT CTTCCAACAA 
GTCAAAGCTT CCATTCCAAG TGCAGAAAAG ACCTCCTCAA CTGATGCAGG TCCAGCTGAG 
GAATTTCCTG AACCTGAGGA AAATGCTGCA AGAGAAGAAA GGAAATTAAA AGACGACACG 
GAGAAAAGGG AACAGAAGGA TGAAGAAATC CCTGAAAATG CAAACAATGA AAAGATGTCC 
ATGGAAACAC TACTCAAAGT GTTCAAAGGG GGAAGTGAAG CACAGGACTC CAATAGATTT 
GCCAGCCACC TAAAGATAGA GAACATTTAT GCAGAGGGCT TCATAAAAAC ATTTCAAGAC 
CTAGGTGCCA AGAACCTGGA GCCAATTGAA GTCGCTGTTC TCTTGAAGCA TCCTTTTATT 
CAAGACCTGA TTTCAAATTA TTCAGACTAT AAGTTTCCTG TACACATCAT CTATGTAGTG 
GAAATGATAG TGTGTGCAAA AGGTTGGGCA CCACTTCCAT CAGGAAAACA GTGTGCAAGA 
TGGAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_005248377.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248434.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

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CloneID	OHu39376
Clone ID Related Accession (Same CDS sequence)	XM_005248377.4 , XM_005248377.3
Accession Version	XM_005248377.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005248377.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248434.1
CDS	104..5560
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_005248377.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248377.4

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_005248377.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248434.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

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CloneID	OHu39376
Clone ID Related Accession (Same CDS sequence)	XM_005248377.4 , XM_005248377.3
Accession Version	XM_005248377.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248434.1
CDS	217..5673
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_005248377.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248377.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGCATAATA 
GGAAATAAAG CATTTACTGA CATTCTGATC GATTTGGTGA CCCTGAACCT TGGCACAAAC 
AACTTTCCTA GTAATTGGAT GCACCTTACC CAACCTGAAT TACAGGAATT AACATCTTTA 
TTAACAGTCA ACTCCGAGTT CGTGGACTGG CGGAAGTTCC TGTTAGTAAC CTCAATGCCT 
TGGCCCATTC CCTTGGAGGA GGAGCTCCTT GAGACCCTTC AGAAGTTCAA GGCTGTGGAT 
AAGGAGCAGT TGGGCACCAT CACTTTTGAG CAGTATATGC AGGCTGGTCT GTGGTTTACA 
GGAGATGAAG ATATAAAAAT TCCAGAAAAT CCTCTTGAAC CCCTTCCATT TAATAGGCAG 
GAGCATCTTA TAGAGTTTTT CTTTAGGCTA TTTGCTGACT ATGAGAAGGA TCCACCCCAG 
CTTGACTACA CACAGATGCT GCTTTACTTT GCTTGCCACC CAGACACCGT GGAAGGAGTC 
TACAGGGCCC TCAGTGTGGC TGTTGGAACT CATGTCTTCC AACAAGTCAA AGCTTCCATT 
CCAAGTGCAG AAAAGACCTC CTCAACTGAT GCAGGTCCAG CTGAGGAATT TCCTGAACCT 
GAGGAAAATG CTGCAAGAGA AGAAAGGAAA TTAAAAGACG ACACGGAGAA AAGGGAACAG 
AAGGATGAAG AAATCCCTGA AAATGCAAAC AATGAAAAGA TGTCCATGGA AACACTACTC 
AAAGTGTTCA AAGGGGGAAG TGAAGCACAG GACTCCAATA GATTTGCCAG CCACCTAAAG 
ATAGAGAACA TTTATGCAGA GGGCTTCATA AAAACATTTC AAGACCTAGG TGCCAAGAAC 
CTGGAGCCAA TTGAAGTCGC TGTTCTCTTG AAGCATCCTT TTATTCAAGA CCTGATTTCA 
AATTATTCAG ACTATAAGTT TCCTGTACAC ATCATCTATG TAGTGGAAAT GATAGTGTGT 
GCAAAAGGTT GGGCACCACT TCCATCAGGA AAACAGTGTG CAAGATGGAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514141.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512443.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

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CloneID	OHu72409
Clone ID Related Accession (Same CDS sequence)	XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
Accession Version	XM_011514141.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514141.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512443.1
CDS	1253..7120
Translation	MIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514141.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514141.3

ORF Insert Sequence:

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017009881.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865370.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

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CloneID	OHu72409
Clone ID Related Accession (Same CDS sequence)	XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
Accession Version	XM_017009881.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865370.1
CDS	1502..7369
Translation	MIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_017009881.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009881.1

ORF Insert Sequence:

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514141.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512443.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

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CloneID	OHu72409
Clone ID Related Accession (Same CDS sequence)	XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
Accession Version	XM_011514141.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514141.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512443.1
CDS	846..6713
Translation	MIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514141.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514141.2

ORF Insert Sequence:

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017009881.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865370.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

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CloneID	OHu72409
Clone ID Related Accession (Same CDS sequence)	XM_011514141.3 , XM_017009881.1 , XM_011514141.2 , XM_017009881.2
Accession Version	XM_017009881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865370.1
CDS	1908..7775
Translation	MIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_017009881.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009881.2

ORF Insert Sequence:

ATGATACCAC GTCAAACTGA TTTCAATCTG ATGCGGATTA CATACAGGTT TCAAGAAAAA 
TATAAACACG TGAAAGAAGA TCTTGCCCAT TTGCATTTTG AGAAACTTGA AAGATTTCAA 
AAACTCAAGG AAGAGCAAAG ATGTTTTGAT ATTGAAAAGC AATACTTAAA CAGAAGACGA 
CAAAATGAAA TAATGGCCAA AATCCAAGCA GCTATTATAC AGATTCCTAA ACCTGCATCA 
AATCGTACTT TGAAAGCACT CGAGGCCCAA AAAATGATGA AAAAGAAAAA AGAGGCAGAA 
GATGTGGCTG ATGAAATTAA GAAGTTCGAA GCATTAATAA AAAAGGATCT CCAAGCAAAA 
GAAAGTGCAT CCAAGACTTC TTTAGATACA GCAGGCCAGA CAACCACCGA TTTGTTAAAT 
ACTTACTCAG ATGACGAGTA CATTAAAAAA ATCCAGAAGC GCCTTGAAGA AGATGCTTTT 
GCACGAGAGC AAAGGGAGAA AAGACGGCGG AAATTGTTAA TGGACCAGTT AATAGCCCAC 
GAAGCACAAG AGGAGGCTTA TCGGGAGGAA CAGCTGATTA ACCGGCTGAT GCGGCAGTCC 
CAGCAGGAGC GCAGGATTGC CGTGCAGCTC ATGCATGTTC GGCATGAAAA GGAAGTTTTA 
TGGCAAAACA GAATTTTCAG AGAAAAACAA CATGAGGAAA GACGACTTAA AGATTTCCAG 
GATGCTCTTG ATCGAGAAGC GGCTTTGGCA AAACAAGCCA AGATTGACTT TGAAGAACAA 
TTCCTTAAAG AAAAGAGATT TCATGATCAG ATTGCTGTGG AAAGAGCTCA AGCTCGTTAT 
GAAAAGCATT ATTCAGTATG TGCAGAAATT TTGGATCAAA TAGTTGATTT GTCCACTAAA 
GTGGCAGACT ATCGAATGTT GACAAATAAT CTGATTCCGT ATAAGTTGAT GCATGATTGG 
AAGGAACTAT TTTTTAATGC AAAACCCATA TATGAACAAG CCTCTGTTAA GACACTACCT 
GCTAACCCCT CAAGAGAACA ACTTACAGAA CTGGAGAAAA GGGACTTGCT AGATACCAAT 
GATTATGAAG AATATAAGAA CATGGTTGGA GAGTGGGCCT TACCAGAAGA AATGGTTGAC 
AATTTACCAC CCTCCAACAA TTGCATACTG GGCCATATTC TTCACAGGCT AGCTGAAAAA 
TCTCTTCCTC CTCGAGCGGA ATCAACAACA CCTGAATTAC CTTCATTTGC TGTTAAAGGA 
TGCTTATTGG GGAAAACATT AAGTGGAAAA ACTACCATTT TAAGGTCTCT ACAAAAAGAC 
TTTCCTATAC AGATACTTTC TATTGACACT CTTGTCCAAG AAGCTATCCA AGCATTTCAT 
GACAATGAAA AAGTCAGTGA GGTTCTACCA ATTCAGAAAA ATGATGAAGA AGATGCTCTA 
CCAGTTCTGC AAGAGGAGAT TAAAGAAAGC CAGGATCCAC AACATGTATT TTCAGCTGGT 
CCAGTTTCAG ATGAAGTATT ACCAGAAACA GAAGGTGAAA CAATGCTTAG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514142.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512444.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

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CloneID	OHu72410
Clone ID Related Accession (Same CDS sequence)	XM_011514142.1
Accession Version	XM_011514142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGATAT TAAAATAATT CTCCAAAGGA GTGAACATGT ACAAGGAAGT 
GATGGAGAGA GATCACCTTC AAGACATACA GAGGAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512444.1
CDS	217..5661
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_011514142.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514142.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGATAT TAAAATAATT CTCCAAAGGA GTGAACATGT ACAAGGAAGT 
GATGGAGAGA GATCACCTTC AAGACATACA GAGGAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017009880.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865369.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

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CloneID	OHu96093
Clone ID Related Accession (Same CDS sequence)	XM_017009880.2 , XM_017009880.1
Accession Version	XM_017009880.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865369.1
CDS	632..6739
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQEQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_017009880.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009880.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017009880.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865369.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

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CloneID	OHu96093
Clone ID Related Accession (Same CDS sequence)	XM_017009880.2 , XM_017009880.1
Accession Version	XM_017009880.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865369.1
CDS	224..6331
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQEQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_017009880.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009880.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGCTAATGCT 
GATAAAACAC CAAAAGCTGA AGAAGTCAAA TCAAGTGATA GTTTCTTAAA ACTCACTACA 
CGTGCTCAGC TTGGTGCAAA ATCAGAACAG TTGCTGAAGA AAGGAAAGAG CATTCCTGAT 
GTGCTGCTTG TTGACATCAT AGTAAATGCT ATTAATGAGA TACCTGTGAA TCAAGACTGT 
ATCCTAGATG GTTTTCCAAT GACTTTAAAC CAAGCACAGC TTCTGGAAGA AGCTCTTACA 
GGCTGCAATA GAAACCTCAC AGAAGTGGAA AGAAAAAAAG CACAAAAATC CACATTGGCT 
ATTGATCCTG CGACTTCCAA AGAAATACCT CTTCCCTCTC CTGCATTTGA TTTTGTCATA 
TTATTAGATG TTTCAGATAC TTCCTCAATG AGTCGCATGA ATGATATTAT AGCTGAAGAA 
TTGTCCTATA AAACTGCTCA CGAAGATATC AGTCAACGTG TAGCTGCTGA AAACCAAGAT 
AAGGATGGAG ACCAAAATTT AAGAGACCAG ATACAACATA GAATTATAGG CTTCTTGGAC 
AACTGGCCTT TATTGGAGCA ATGGTTTTCA GAGCCAGAAA ATATTTTGAT AAAAATCAAT 
GCTGAAATAG ATAAGGAATC TTTATGTGAA AAAGTAAAAG AAATTCTTAC GACTGAAATA 
GCAAAAAAAA AGAATAAAGT TGAGAAGAAA TTAGAAGAAA AGGAAGCTGA GAAAAAAGCA 
GCAGCTTCCC TGGCTGAGCT TCCACTTCCT ACACCTCCTC CTGCTCCTCC TCCTGAACCA 
GAAAAAGAGA AGGAAATTCA TCAAAGCCAT GTGGCTTCAA AAACTCCTAC TGCAAAAGGA 
AAACCTCAAT CAGAAGCCCC GCATGGTAAG CAAGAATCTC TTCAGGAAGG AAAAGGGAAG 
AAAGGTGAGA CCGCACTCAA AAGAAAAGGT TCTCCTAAAG GAAAATCATC AGGAGGAAAA 
GTACCAGTAA AGAAATCACC TGCTGACTCT ACAGATACAT CACCTGTTGC AATAGTGCCA 
CAGCCACCTA AGCCAGGATC AGAAGAATGG GTCTATGTGA ATGAACCAGT TCCTGAGGAA 
ATGCCTTTGT TTTTAGTACC TTACTGGGAA CTAATAGAAA ATTCCTATAT AAACACCATC 
AAAACAGTAC TCAGGCATCT GAGGGAAGAC CAGCATACTG TGCTTGCTTA CTTATATGAG 
ATTAGAACAA GTTTCCAGGA GTTTCTAAAG CGTCCGGATC ACAAGCAAGA TTTTGTAGCT 
CAATGGCAGG CTGATTTCAA CTCCCTTCCT GATGACCTGT GGGATGATGA GGAAACAAAG 
GCTGAACTAC ATCAACGAGT GAATGATCTG CGAGACCGCC TGTGGGACAT TTGTGATGCC 
CGGAAGGAAG AGGCGGAGCA GGAGCGGCTT GACATCATTA ATGAGAGCTG GTTACAGGAC 
ACTCTTGGAA TGACAATGAA CCATTTCTTT TCCCTGATGC AGGCAGAGCT GAACCGTTTC 
CAAGATACAA AGAGACTCCT TCAAGATTAT TACTGGGGAA TGGAAAGTAA AATCCCAGTA 
GAGGACAACA AGAGATTTAC TCGAATCCCT TTGGTCCAAC TGGATAGTAA AGACAATTCT 
GAAAGCCAGC TTAGAATTCC TCTAGTTCCT CGAATATCCA TTTCTCTGGA AACAGTTACA 
CCCAAACCAA AAACAAAATC AGTACTGAAG GGCAAGATGG ATAACTCCCT AGAAAACGTT 
GAGTCCAACT TTGAGGCCGA TGAAAAGTTG GTCATGGACA CCTGGCAGCA GGCTTCTTTA 
GCAGTATCTC ACATGGTGGC TGCTGAAATT CATCAGAGGC TTATGGAAGA AGAAAAAGAA 
AACCAGCCAG CAGACCCCAA AGAAAAATCT CCTCAGATGG GTGCAAATAA AAAAGTCAAA 
AAGGAGCCAC CCAAGAAAAA ACAGGAAGAC AAAAAACCCA AAGGTAAATC ACCACCTATG 
GCAGAAGCAA CTCCTGTCAT AGTAACAACA GAGGAAATTG CTGAAATCAA AAGGAAAAAT 
GAACTGAGGG TCAAAATAAA AGAAGAACAC CTTGCTGCCT TGCAATTTGA AGAAATAGCC 
ACGCAATTTC GACTTGAACT GATAAAGACA AAAGCATTGG CTCTTCTTGA AGATTTAGTA 
ACAAAGGTGG TTGATGTATA TAAACTCATG GAAAAATGGC TTGGTGAGAG GTATTTGAAT 
GAAATGGCCA GTACAGAAAA ATTAACTGAC GTAGCTCGCT ATCACATTGA AACATCTACA 
AAAATTCAGA ATGAACTTTA TTTAAGCCAA GAAGACTTCT TCATTAATGG CAATATAAAA 
GTCTTCCCAG ATCCTCCCCC ATCAATACGT CCTCCACCTG TAGAAAAGGA AGAAGATGGT 
ACCCTGACCA TTGAACAGCT TGACAGTCTT CGAGATCAGT TCTTAGATAT GGCACCTAAA 
GGAGACAGGA AGTGGCTTCA TGGATTACAG GCAGCCCTCC AGAACTTTCT CAAGGGATAT 
CCTGCAATTG TTCGGCAACT GCACTCAGCA GGCAGAGGCA AAAGTGACAC CAGTCAAAAG 
AAACCCAAAG AACTTCTGGA GTCCCTTCTC CAAGCTGACA TTGTTAAATT TGCCCACTTC 
TTGTTGGATG TCATGAATGT CCTTAGCATT CTGTCCTGTG TCAATAAGAA TAGAAATTCC 
TCAATTGCTG ACATATTTGC CACTTTAGAG TCAATGCTGG AAATGCTCCA AATGTATCAA 
ACAAGACTAG GGCCAAAAGA ACGCATGGTG GATTCAGCCA CACACTTTCA TGGTAACTGC 
CTCAGAGGGA AAGAAAACAT TTCTGCTGTG AGAAACATTG TTTTAACACA TCTTATCAAG 
AGACTTCAAG GCTGTTTCAG AGATGCCAGC CAAAATGTGG TGAGGGCTAC CGTGATTGGA 
AGTTTTAAAT TGTGGCCCAC AAAGATAAAT CAAGAATTTG GAGAAAAAGA GGTATCCATC 
CTGACTGCAC ATTATGAACC AGTATTAGAA GCTGCCAATG TGAAACTTAG TGAAGTGGAT 
ACTGAATGGA GCATGTTGAA ATTAGAGATT TATGCCAGAT TTCAGAACAT TCGAAAATTG 
ACCTGGGATT TTGTGAATTC CATTTACTTA CACAAGTATC CAAATATCCT GACACTAGTC 
GACCTAGTGT TGACCCTTCC TGCAAGTTCA GCTGAAGCAG AACATGGCTT TAGTCAGATG 
AAATGGACCA AGTCACATAT GCATGCAAAA ATCAAGGCTG AAAGCATAAT AGGAAATAAA 
GCATTTACTG ACATTCTGAT CGATTTGGTG ACCCTGAACC TTGGCACAAA CAACTTTCCT 
AGTAATTGGA TGCACCTTAC CCAACCTGAA TTACAGGAAT TAACATCTTT ATTAACAGTC 
AACTCCGAGT TCGTGGACTG GCGGAAGTTC CTGTTAGTAA CCTCAATGCC TTGGCCCATT 
CCCTTGGAGG AGGAGCTCCT TGAGACCCTT CAGAAGTTCA AGGCTGTGGA TAAGGAGCAG 
TTGGGCACCA TCACTTTTGA GCAGTATATG CAGGCTGGTC TGTGGTTTAC AGGAGATGAA 
GATATAAAAA TTCCAGAAAA TCCTCTTGAA CCCCTTCCAT TTAATAGGCA GGAGCATCTT 
ATAGAGTTTT TCTTTAGGCT ATTTGCTGAC TATGAGAAGG ATCCACCCCA GCTTGACTAC 
ACACAGATGC TGCTTTACTT TGCTTGCCAC CCAGACACCG TGGAAGGAGT CTACAGGGCC 
CTCAGTGTGG CTGTTGGAAC TCATGTCTTC CAACAAGTCA AAGCTTCCAT TCCAAGTGCA 
GAAAAGACCT CCTCAACTGA TGCAGGTCCA GCTGAGGAAT TTCCTGAACC TGAGGAAAAT 
GCTGCAAGAG AAGAAAGGAA ATTAAAAGAC GACACGGAGA AAAGGGAACA GAAGGATGAA 
GAAATCCCTG AAAATGCAAA CAATGAAAAG ATGTCCATGG AAACACTACT CAAAGTGTTC 
AAAGGGGGAA GTGAAGCACA GGACTCCAAT AGATTTGCCA GCCACCTAAA GATAGAGAAC 
ATTTATGCAG AGGGCTTCAT AAAAACATTT CAAGACCTAG GTGCCAAGAA CCTGGAGCCA 
ATTGAAGTCG CTGTTCTCTT GAAGCATCCT TTTATTCAAG ACCTGATTTC AAATTATTCA 
GACTATAAGT TTCCTGTACA CATCATCTAT GTAGTGGAAA TGATAGTGTG TGCAAAAGGT 
TGGGCACCAC TTCCATCAGG AAAACAGTGT GCAAGATGGA GAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514135.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512437.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

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CloneID	OHu72403
Clone ID Related Accession (Same CDS sequence)	XM_011514135.2 , XM_011514135.3
Accession Version	XM_011514135.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514135.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512437.1
CDS	222..6515
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514135.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514135.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514135.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512437.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

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CloneID	OHu72403
Clone ID Related Accession (Same CDS sequence)	XM_011514135.2 , XM_011514135.3
Accession Version	XM_011514135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514135.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512437.1
CDS	104..6397
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514135.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514135.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGTACACATC ATCTATGTAG TGGAAATGAT AGTGTGTGCA 
AAAGGTTGGG CACCACTTCC ATCAGGAAAA CAGTGTGCAA GATGGAGAAA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514136.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512438.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OHu72404
Clone ID Related Accession (Same CDS sequence)	XM_011514136.2 , XM_011514136.3
Accession Version	XM_011514136.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514136.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA 
GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512438.1
CDS	221..6511
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_011514136.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514136.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA 
GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514136.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512438.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OHu72404
Clone ID Related Accession (Same CDS sequence)	XM_011514136.2 , XM_011514136.3
Accession Version	XM_011514136.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514136.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA 
GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512438.1
CDS	104..6394
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPDIKIILQRSEHVQGSDGERSPSRHTEEKK

Target ORF information:

RefSeq Version	XM_011514136.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514136.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTGAGACCGC ACTCAAAAGA AAAGGTTCTC CTAAAGGAAA ATCATCAGGA 
GGAAAAGTAC CAGTAAAGAA ATCACCTGCT GACTCTACAG ATACATCACC TGTTGCAATA 
GTGCCACAGC CACCTAAGCC AGGATCAGAA GAATGGGTCT ATGTGAATGA ACCAGTTCCT 
GAGGAAATGC CTTTGTTTTT AGTACCTTAC TGGGAACTAA TAGAAAATTC CTATATAAAC 
ACCATCAAAA CAGTACTCAG GCATCTGAGG GAAGACCAGC ATACTGTGCT TGCTTACTTA 
TATGAGATTA GAACAAGTTT CCAGGAGTTT CTAAAGCGTC CGGATCACAA GCAAGATTTT 
GTAGCTCAAT GGCAGGCTGA TTTCAACTCC CTTCCTGATG ACCTGTGGGA TGATGAGGAA 
ACAAAGGCTG AACTACATCA ACGAGTGAAT GATCTGCGAG ACCGCCTGTG GGACATTTGT 
GATGCCCGGA AGGAAGAGGC GGAGCAGGAG CGGCTTGACA TCATTAATGA GAGCTGGTTA 
CAGGACACTC TTGGAATGAC AATGAACCAT TTCTTTTCCC TGATGCAGGC AGAGCTGAAC 
CGTTTCCAAG ATACAAAGAG ACTCCTTCAA GATTATTACT GGGGAATGGA AAGTAAAATC 
CCAGTAGAGG ACAACAAGAG ATTTACTCGA ATCCCTTTGG TCCAACTGGA TAGTAAAGAC 
AATTCTGAAA GCCAGCTTAG AATTCCTCTA GTTCCTCGAA TATCCATTTC TCTGGAAACA 
GTTACACCCA AACCAAAAAC AAAATCAGTA CTGAAGGGCA AGATGGATAA CTCCCTAGAA 
AACGTTGAGT CCAACTTTGA GGCCGATGAA AAGTTGGTCA TGGACACCTG GCAGCAGGCT 
TCTTTAGCAG TATCTCACAT GGTGGCTGCT GAAATTCATC AGAGGCTTAT GGAAGAAGAA 
AAAGAAAACC AGCCAGCAGA CCCCAAAGAA AAATCTCCTC AGATGGGTGC AAATAAAAAA 
GTCAAAAAGG AGCCACCCAA GAAAAAACAG GAAGACAAAA AACCCAAAGG TAAATCACCA 
CCTATGGCAG AAGCAACTCC TGTCATAGTA ACAACAGAGG AAATTGCTGA AATCAAAAGG 
AAAAATGAAC TGAGGGTCAA AATAAAAGAA GAACACCTTG CTGCCTTGCA ATTTGAAGAA 
ATAGCCACGC AATTTCGACT TGAACTGATA AAGACAAAAG CATTGGCTCT TCTTGAAGAT 
TTAGTAACAA AGGTGGTTGA TGTATATAAA CTCATGGAAA AATGGCTTGG TGAGAGGTAT 
TTGAATGAAA TGGCCAGTAC AGAAAAATTA ACTGACGTAG CTCGCTATCA CATTGAAACA 
TCTACAAAAA TTCAGAATGA ACTTTATTTA AGCCAAGAAG ACTTCTTCAT TAATGGCAAT 
ATAAAAGTCT TCCCAGATCC TCCCCCATCA ATACGTCCTC CACCTGTAGA AAAGGAAGAA 
GATGGTACCC TGACCATTGA ACAGCTTGAC AGTCTTCGAG ATCAGTTCTT AGATATGGCA 
CCTAAAGGAG ACAGGAAGTG GCTTCATGGA TTACAGGCAG CCCTCCAGAA CTTTCTCAAG 
GGATATCCTG CAATTGTTCG GCAACTGCAC TCAGCAGGCA GAGGCAAAAG TGACACCAGT 
CAAAAGAAAC CCAAAGAACT TCTGGAGTCC CTTCTCCAAG CTGACATTGT TAAATTTGCC 
CACTTCTTGT TGGATGTCAT GAATGTCCTT AGCATTCTGT CCTGTGTCAA TAAGAATAGA 
AATTCCTCAA TTGCTGACAT ATTTGCCACT TTAGAGTCAA TGCTGGAAAT GCTCCAAATG 
TATCAAACAA GACTAGGGCC AAAAGAACGC ATGGTGGATT CAGCCACACA CTTTCATGGT 
AACTGCCTCA GAGGGAAAGA AAACATTTCT GCTGTGAGAA ACATTGTTTT AACACATCTT 
ATCAAGAGAC TTCAAGGCTG TTTCAGAGAT GCCAGCCAAA ATGTGGTGAG GGCTACCGTG 
ATTGGAAGTT TTAAATTGTG GCCCACAAAG ATAAATCAAG AATTTGGAGA AAAAGAGGTA 
TCCATCCTGA CTGCACATTA TGAACCAGTA TTAGAAGCTG CCAATGTGAA ACTTAGTGAA 
GTGGATACTG AATGGAGCAT GTTGAAATTA GAGATTTATG CCAGATTTCA GAACATTCGA 
AAATTGACCT GGGATTTTGT GAATTCCATT TACTTACACA AGTATCCAAA TATCCTGACA 
CTAGTCGACC TAGTGTTGAC CCTTCCTGCA AGTTCAGCTG AAGCAGAACA TGGCTTTAGT 
CAGATGAAAT GGACCAAGTC ACATATGCAT GCAAAAATCA AGGCTGAAAG CATAATAGGA 
AATAAAGCAT TTACTGACAT TCTGATCGAT TTGGTGACCC TGAACCTTGG CACAAACAAC 
TTTCCTAGTA ATTGGATGCA CCTTACCCAA CCTGAATTAC AGGAATTAAC ATCTTTATTA 
ACAGTCAACT CCGAGTTCGT GGACTGGCGG AAGTTCCTGT TAGTAACCTC AATGCCTTGG 
CCCATTCCCT TGGAGGAGGA GCTCCTTGAG ACCCTTCAGA AGTTCAAGGC TGTGGATAAG 
GAGCAGTTGG GCACCATCAC TTTTGAGCAG TATATGCAGG CTGGTCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TTCCATTTAA TAGGCAGGAG 
CATCTTATAG AGTTTTTCTT TAGGCTATTT GCTGACTATG AGAAGGATCC ACCCCAGCTT 
GACTACACAC AGATGCTGCT TTACTTTGCT TGCCACCCAG ACACCGTGGA AGGAGTCTAC 
AGGGCCCTCA GTGTGGCTGT TGGAACTCAT GTCTTCCAAC AAGTCAAAGC TTCCATTCCA 
AGTGCAGAAA AGACCTCCTC AACTGATGCA GGTCCAGCTG AGGAATTTCC TGAACCTGAG 
GAAAATGCTG CAAGAGAAGA AAGGAAATTA AAAGACGACA CGGAGAAAAG GGAACAGAAG 
GATGAAGAAA TCCCTGAAAA TGCAAACAAT GAAAAGATGT CCATGGAAAC ACTACTCAAA 
GTGTTCAAAG GGGGAAGTGA AGCACAGGAC TCCAATAGAT TTGCCAGCCA CCTAAAGATA 
GAGAACATTT ATGCAGAGGG CTTCATAAAA ACATTTCAAG ACCTAGGTGC CAAGAACCTG 
GAGCCAATTG AAGTCGCTGT TCTCTTGAAG CATCCTTTTA TTCAAGACCT GATTTCAAAT 
TATTCAGACT ATAAGTTTCC TGATATTAAA ATAATTCTCC AAAGGAGTGA ACATGTACAA 
GGAAGTGATG GAGAGAGATC ACCTTCAAGA CATACAGAGG AAAAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514137.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512439.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OHu72405
Clone ID Related Accession (Same CDS sequence)	XM_011514137.2 , XM_011514137.3
Accession Version	XM_011514137.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514137.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512439.1
CDS	221..6499
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514137.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514137.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514137.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512439.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OHu72405
Clone ID Related Accession (Same CDS sequence)	XM_011514137.2 , XM_011514137.3
Accession Version	XM_011514137.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512439.1
CDS	104..6382
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514137.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514137.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGCTAATGC TGATAAAACA 
CCAAAAGCTG AAGAAGTCAA ATCAAGTGAT AGTTTCTTAA AACTCACTAC ACGTGCTCAG 
CTTGGTGCAA AATCAGAACA GTTGCTGAAG AAAGGAAAGA GCATTCCTGA TGTGCTGCTT 
GTTGACATCA TAGTAAATGC TATTAATGAG ATACCTGTGA ATCAAGACTG TATCCTAGAT 
GGTTTTCCAA TGACTTTAAA CCAAGCACAG CTTCTGGAAG AAGCTCTTAC AGGCTGCAAT 
AGAAACCTCA CAGAAGTGGA AAGAAAAAAA GCACAAAAAT CCACATTGGC TATTGATCCT 
GCGACTTCCA AAGAAATACC TCTTCCCTCT CCTGCATTTG ATTTTGTCAT ATTATTAGAT 
GTTTCAGATA CTTCCTCAAT GAGTCGCATG AATGATATTA TAGCTGAAGA ATTGTCCTAT 
AAAACTGCTC ACGAAGATAT CAGTCAACGT GTAGCTGCTG AAAACCAAGA TAAGGATGGA 
GACCAAAATT TAAGAGACCA GATACAACAT AGAATTATAG GCTTCTTGGA CAACTGGCCT 
TTATTGGAGC AATGGTTTTC AGAGCCAGAA AATATTTTGA TAAAAATCAA TGCTGAAATA 
GATAAGGAAT CTTTATGTGA AAAAGTAAAA GAAATTCTTA CGACTGAAAT AGCAAAAAAA 
AAGAATAAAG TTGAGAAGAA ATTAGAAGAA AAGGAAGCTG AGAAAAAAGC AGCAGCTTCC 
CTGGCTGAGC TTCCACTTCC TACACCTCCT CCTGCTCCTC CTCCTGAACC AGAAAAAGAG 
AAGGAAATTC ATCAAAGCCA TGTGGCTTCA AAAACTCCTA CTGCAAAAGG AAAACCTCAA 
TCAGAAGCCC CGCATGGTAA GCAAGAATCT CTTCAGGAAG GAAAAGGGAA GAAAGGTGAG 
ACCGCACTCA AAAGAAAAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514138.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512440.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

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CloneID	OHu72406
Clone ID Related Accession (Same CDS sequence)	XM_011514138.2 , XM_011514138.3
Accession Version	XM_011514138.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514138.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT 
CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA 
CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG 
GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT 
GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT 
GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA 
GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC 
ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC 
AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC 
ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA 
CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG 
AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT 
TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT 
CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT 
ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512440.1
CDS	222..6491
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514138.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514138.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT 
CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA 
CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG 
GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT 
GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT 
GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA 
GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC 
ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC 
AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC 
ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA 
CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG 
AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT 
TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT 
CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT 
ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514138.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512440.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

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CloneID	OHu72406
Clone ID Related Accession (Same CDS sequence)	XM_011514138.2 , XM_011514138.3
Accession Version	XM_011514138.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514138.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT 
CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA 
CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG 
GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT 
GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT 
GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA 
GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC 
ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC 
AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC 
ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA 
CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG 
AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT 
TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT 
CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT 
ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512440.1
CDS	104..6373
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514138.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514138.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGA AGCCCCGCAT GGTAAGCAAG AATCTCTTCA GGAAGGAAAA 
GGGAAGAAAG GTTCTCCTAA AGGAAAATCA TCAGGAGGAA AAGTACCAGT AAAGAAATCA 
CCTGCTGACT CTACAGATAC ATCACCTGTT GCAATAGTGC CACAGCCACC TAAGCCAGGA 
TCAGAAGAAT GGGTCTATGT GAATGAACCA GTTCCTGAGG AAATGCCTTT GTTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCCTAT ATAAACACCA TCAAAACAGT ACTCAGGCAT 
CTGAGGGAAG ACCAGCATAC TGTGCTTGCT TACTTATATG AGATTAGAAC AAGTTTCCAG 
GAGTTTCTAA AGCGTCCGGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCCCTTC CTGATGACCT GTGGGATGAT GAGGAAACAA AGGCTGAACT ACATCAACGA 
GTGAATGATC TGCGAGACCG CCTGTGGGAC ATTTGTGATG CCCGGAAGGA AGAGGCGGAG 
CAGGAGCGGC TTGACATCAT TAATGAGAGC TGGTTACAGG ACACTCTTGG AATGACAATG 
AACCATTTCT TTTCCCTGAT GCAGGCAGAG CTGAACCGTT TCCAAGATAC AAAGAGACTC 
CTTCAAGATT ATTACTGGGG AATGGAAAGT AAAATCCCAG TAGAGGACAA CAAGAGATTT 
ACTCGAATCC CTTTGGTCCA ACTGGATAGT AAAGACAATT CTGAAAGCCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCTG GAAACAGTTA CACCCAAACC AAAAACAAAA 
TCAGTACTGA AGGGCAAGAT GGATAACTCC CTAGAAAACG TTGAGTCCAA CTTTGAGGCC 
GATGAAAAGT TGGTCATGGA CACCTGGCAG CAGGCTTCTT TAGCAGTATC TCACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTTATGGAA GAAGAAAAAG AAAACCAGCC AGCAGACCCC 
AAAGAAAAAT CTCCTCAGAT GGGTGCAAAT AAAAAAGTCA AAAAGGAGCC ACCCAAGAAA 
AAACAGGAAG ACAAAAAACC CAAAGGTAAA TCACCACCTA TGGCAGAAGC AACTCCTGTC 
ATAGTAACAA CAGAGGAAAT TGCTGAAATC AAAAGGAAAA ATGAACTGAG GGTCAAAATA 
AAAGAAGAAC ACCTTGCTGC CTTGCAATTT GAAGAAATAG CCACGCAATT TCGACTTGAA 
CTGATAAAGA CAAAAGCATT GGCTCTTCTT GAAGATTTAG TAACAAAGGT GGTTGATGTA 
TATAAACTCA TGGAAAAATG GCTTGGTGAG AGGTATTTGA ATGAAATGGC CAGTACAGAA 
AAATTAACTG ACGTAGCTCG CTATCACATT GAAACATCTA CAAAAATTCA GAATGAACTT 
TATTTAAGCC AAGAAGACTT CTTCATTAAT GGCAATATAA AAGTCTTCCC AGATCCTCCC 
CCATCAATAC GTCCTCCACC TGTAGAAAAG GAAGAAGATG GTACCCTGAC CATTGAACAG 
CTTGACAGTC TTCGAGATCA GTTCTTAGAT ATGGCACCTA AAGGAGACAG GAAGTGGCTT 
CATGGATTAC AGGCAGCCCT CCAGAACTTT CTCAAGGGAT ATCCTGCAAT TGTTCGGCAA 
CTGCACTCAG CAGGCAGAGG CAAAAGTGAC ACCAGTCAAA AGAAACCCAA AGAACTTCTG 
GAGTCCCTTC TCCAAGCTGA CATTGTTAAA TTTGCCCACT TCTTGTTGGA TGTCATGAAT 
GTCCTTAGCA TTCTGTCCTG TGTCAATAAG AATAGAAATT CCTCAATTGC TGACATATTT 
GCCACTTTAG AGTCAATGCT GGAAATGCTC CAAATGTATC AAACAAGACT AGGGCCAAAA 
GAACGCATGG TGGATTCAGC CACACACTTT CATGGTAACT GCCTCAGAGG GAAAGAAAAC 
ATTTCTGCTG TGAGAAACAT TGTTTTAACA CATCTTATCA AGAGACTTCA AGGCTGTTTC 
AGAGATGCCA GCCAAAATGT GGTGAGGGCT ACCGTGATTG GAAGTTTTAA ATTGTGGCCC 
ACAAAGATAA ATCAAGAATT TGGAGAAAAA GAGGTATCCA TCCTGACTGC ACATTATGAA 
CCAGTATTAG AAGCTGCCAA TGTGAAACTT AGTGAAGTGG ATACTGAATG GAGCATGTTG 
AAATTAGAGA TTTATGCCAG ATTTCAGAAC ATTCGAAAAT TGACCTGGGA TTTTGTGAAT 
TCCATTTACT TACACAAGTA TCCAAATATC CTGACACTAG TCGACCTAGT GTTGACCCTT 
CCTGCAAGTT CAGCTGAAGC AGAACATGGC TTTAGTCAGA TGAAATGGAC CAAGTCACAT 
ATGCATGCAA AAATCAAGGC TGAAAGCATA ATAGGAAATA AAGCATTTAC TGACATTCTG 
ATCGATTTGG TGACCCTGAA CCTTGGCACA AACAACTTTC CTAGTAATTG GATGCACCTT 
ACCCAACCTG AATTACAGGA ATTAACATCT TTATTAACAG TCAACTCCGA GTTCGTGGAC 
TGGCGGAAGT TCCTGTTAGT AACCTCAATG CCTTGGCCCA TTCCCTTGGA GGAGGAGCTC 
CTTGAGACCC TTCAGAAGTT CAAGGCTGTG GATAAGGAGC AGTTGGGCAC CATCACTTTT 
GAGCAGTATA TGCAGGCTGG TCTGTGGTTT ACAGGAGATG AAGATATAAA AATTCCAGAA 
AATCCTCTTG AACCCCTTCC ATTTAATAGG CAGGAGCATC TTATAGAGTT TTTCTTTAGG 
CTATTTGCTG ACTATGAGAA GGATCCACCC CAGCTTGACT ACACACAGAT GCTGCTTTAC 
TTTGCTTGCC ACCCAGACAC CGTGGAAGGA GTCTACAGGG CCCTCAGTGT GGCTGTTGGA 
ACTCATGTCT TCCAACAAGT CAAAGCTTCC ATTCCAAGTG CAGAAAAGAC CTCCTCAACT 
GATGCAGGTC CAGCTGAGGA ATTTCCTGAA CCTGAGGAAA ATGCTGCAAG AGAAGAAAGG 
AAATTAAAAG ACGACACGGA GAAAAGGGAA CAGAAGGATG AAGAAATCCC TGAAAATGCA 
AACAATGAAA AGATGTCCAT GGAAACACTA CTCAAAGTGT TCAAAGGGGG AAGTGAAGCA 
CAGGACTCCA ATAGATTTGC CAGCCACCTA AAGATAGAGA ACATTTATGC AGAGGGCTTC 
ATAAAAACAT TTCAAGACCT AGGTGCCAAG AACCTGGAGC CAATTGAAGT CGCTGTTCTC 
TTGAAGCATC CTTTTATTCA AGACCTGATT TCAAATTATT CAGACTATAA GTTTCCTGTA 
CACATCATCT ATGTAGTGGA AATGATAGTG TGTGCAAAAG GTTGGGCACC ACTTCCATCA 
GGAAAACAGT GTGCAAGATG GAGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514139.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512441.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

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CloneID	OHu72407
Clone ID Related Accession (Same CDS sequence)	XM_011514139.2 , XM_011514139.3
Accession Version	XM_011514139.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512441.1
CDS	221..6439
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514139.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514139.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514139.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512441.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

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CloneID	OHu72407
Clone ID Related Accession (Same CDS sequence)	XM_011514139.2 , XM_011514139.3
Accession Version	XM_011514139.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011514139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512441.1
CDS	104..6322
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQERLRHMIPRQTDFNLMRITYRFQEKYKHVKEDLAHLHFEKLERFQKLKEEQRCFDIEKQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514139.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514139.3

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGAGACTT 
AGACACATGA TACCACGTCA AACTGATTTC AATCTGATGC GGATTACATA CAGGTTTCAA 
GAAAAATATA AACACGTGAA AGAAGATCTT GCCCATTTGC ATTTTGAGAA ACTTGAAAGA 
TTTCAAAAAC TCAAGGAAGA GCAAAGATGT TTTGATATTG AAAAGCAATA CTTAAACAGA 
AGACGACAAA ATGAAATAAT GGCCAAAATC CAAGCAGCTA TTATACAGAT TCCTAAACCT 
GCATCAAATC GTACTTTGAA AGCACTCGAG GCCCAAAAAA TGATGAAAAA GAAAAAAGAG 
GCAGAAGATG TGGCTGATGA AATTAAGAAG TTCGAAGCAT TAATAAAAAA GGATCTCCAA 
GCAAAAGAAA GTGCATCCAA GACTTCTTTA GATACAGCAG GCCAGACAAC CACCGATTTG 
TTAAATACTT ACTCAGATGA CGAGTACATT AAAAAAATCC AGAAGCGCCT TGAAGAAGAT 
GCTTTTGCAC GAGAGCAAAG GGAGAAAAGA CGGCGGAAAT TGTTAATGGA CCAGTTAATA 
GCCCACGAAG CACAAGAGGA GGCTTATCGG GAGGAACAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATTGCCGTG CAGCTCATGC ATGTTCGGCA TGAAAAGGAA 
GTTTTATGGC AAAACAGAAT TTTCAGAGAA AAACAACATG AGGAAAGACG ACTTAAAGAT 
TTCCAGGATG CTCTTGATCG AGAAGCGGCT TTGGCAAAAC AAGCCAAGAT TGACTTTGAA 
GAACAATTCC TTAAAGAAAA GAGATTTCAT GATCAGATTG CTGTGGAAAG AGCTCAAGCT 
CGTTATGAAA AGCATTATTC AGTATGTGCA GAAATTTTGG ATCAAATAGT TGATTTGTCC 
ACTAAAGTGG CAGACTATCG AATGTTGACA AATAATCTGA TTCCGTATAA GTTGATGCAT 
GATTGGAAGG AACTATTTTT TAATGCAAAA CCCATATATG AACAAGCCTC TGTTAAGACA 
CTACCTGCTA ACCCCTCAAG AGAACAACTT ACAGAACTGG AGAAAAGGGA CTTGCTAGAT 
ACCAATGATT ATGAAGAATA TAAGAACATG GTTGGAGAGT GGGCCTTACC AGAAGAAATG 
GTTGACAATT TACCACCCTC CAACAATTGC ATACTGGGCC ATATTCTTCA CAGGCTAGCT 
GAAAAATCTC TTCCTCCTCG AGCGGAATCA ACAACACCTG AATTACCTTC ATTTGCTGTT 
AAAGGATGCT TATTGGGGAA AACATTAAGT GGAAAAACTA CCATTTTAAG GTCTCTACAA 
AAAGACTTTC CTATACAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TTTCATGACA ATGAAAAAGT CAGTGAGGTT CTACCAATTC AGAAAAATGA TGAAGAAGAT 
GCTCTACCAG TTCTGCAAGA GGAGATTAAA GAAAGCCAGG ATCCACAACA TGTATTTTCA 
GCTGGTCCAG TTTCAGATGA AGTATTACCA GAAACAGAAG GTGAAACAAT GCTTAGTGCT 
AATGCTGATA AAACACCAAA AGCTGAAGAA GTCAAATCAA GTGATAGTTT CTTAAAACTC 
ACTACACGTG CTCAGCTTGG TGCAAAATCA GAACAGTTGC TGAAGAAAGG AAAGAGCATT 
CCTGATGTGC TGCTTGTTGA CATCATAGTA AATGCTATTA ATGAGATACC TGTGAATCAA 
GACTGTATCC TAGATGGTTT TCCAATGACT TTAAACCAAG CACAGCTTCT GGAAGAAGCT 
CTTACAGGCT GCAATAGAAA CCTCACAGAA GTGGAAAGAA AAAAAGCACA AAAATCCACA 
TTGGCTATTG ATCCTGCGAC TTCCAAAGAA ATACCTCTTC CCTCTCCTGC ATTTGATTTT 
GTCATATTAT TAGATGTTTC AGATACTTCC TCAATGAGTC GCATGAATGA TATTATAGCT 
GAAGAATTGT CCTATAAAAC TGCTCACGAA GATATCAGTC AACGTGTAGC TGCTGAAAAC 
CAAGATAAGG ATGGAGACCA AAATTTAAGA GACCAGATAC AACATAGAAT TATAGGCTTC 
TTGGACAACT GGCCTTTATT GGAGCAATGG TTTTCAGAGC CAGAAAATAT TTTGATAAAA 
ATCAATGCTG AAATAGATAA GGAATCTTTA TGTGAAAAAG TAAAAGAAAT TCTTACGACT 
GAAATAGCAA AAAAAAAGAA TAAAGTTGAG AAGAAATTAG AAGAAAAGGA AGCTGAGAAA 
AAAGCAGCAG CTTCCCTGGC TGAGCTTCCA CTTCCTACAC CTCCTCCTGC TCCTCCTCCT 
GAACCAGAAA AAGAGAAGGA AATTCATCAA AGCCATGTGG CTTCAAAAAC TCCTACTGCA 
AAAGGAAAAC CTCAATCAGG TTCTCCTAAA GGAAAATCAT CAGGAGGAAA AGTACCAGTA 
AAGAAATCAC CTGCTGACTC TACAGATACA TCACCTGTTG CAATAGTGCC ACAGCCACCT 
AAGCCAGGAT CAGAAGAATG GGTCTATGTG AATGAACCAG TTCCTGAGGA AATGCCTTTG 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCCTATA TAAACACCAT CAAAACAGTA 
CTCAGGCATC TGAGGGAAGA CCAGCATACT GTGCTTGCTT ACTTATATGA GATTAGAACA 
AGTTTCCAGG AGTTTCTAAA GCGTCCGGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCCCTTCC TGATGACCTG TGGGATGATG AGGAAACAAA GGCTGAACTA 
CATCAACGAG TGAATGATCT GCGAGACCGC CTGTGGGACA TTTGTGATGC CCGGAAGGAA 
GAGGCGGAGC AGGAGCGGCT TGACATCATT AATGAGAGCT GGTTACAGGA CACTCTTGGA 
ATGACAATGA ACCATTTCTT TTCCCTGATG CAGGCAGAGC TGAACCGTTT CCAAGATACA 
AAGAGACTCC TTCAAGATTA TTACTGGGGA ATGGAAAGTA AAATCCCAGT AGAGGACAAC 
AAGAGATTTA CTCGAATCCC TTTGGTCCAA CTGGATAGTA AAGACAATTC TGAAAGCCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCTGG AAACAGTTAC ACCCAAACCA 
AAAACAAAAT CAGTACTGAA GGGCAAGATG GATAACTCCC TAGAAAACGT TGAGTCCAAC 
TTTGAGGCCG ATGAAAAGTT GGTCATGGAC ACCTGGCAGC AGGCTTCTTT AGCAGTATCT 
CACATGGTGG CTGCTGAAAT TCATCAGAGG CTTATGGAAG AAGAAAAAGA AAACCAGCCA 
GCAGACCCCA AAGAAAAATC TCCTCAGATG GGTGCAAATA AAAAAGTCAA AAAGGAGCCA 
CCCAAGAAAA AACAGGAAGA CAAAAAACCC AAAGGTAAAT CACCACCTAT GGCAGAAGCA 
ACTCCTGTCA TAGTAACAAC AGAGGAAATT GCTGAAATCA AAAGGAAAAA TGAACTGAGG 
GTCAAAATAA AAGAAGAACA CCTTGCTGCC TTGCAATTTG AAGAAATAGC CACGCAATTT 
CGACTTGAAC TGATAAAGAC AAAAGCATTG GCTCTTCTTG AAGATTTAGT AACAAAGGTG 
GTTGATGTAT ATAAACTCAT GGAAAAATGG CTTGGTGAGA GGTATTTGAA TGAAATGGCC 
AGTACAGAAA AATTAACTGA CGTAGCTCGC TATCACATTG AAACATCTAC AAAAATTCAG 
AATGAACTTT ATTTAAGCCA AGAAGACTTC TTCATTAATG GCAATATAAA AGTCTTCCCA 
GATCCTCCCC CATCAATACG TCCTCCACCT GTAGAAAAGG AAGAAGATGG TACCCTGACC 
ATTGAACAGC TTGACAGTCT TCGAGATCAG TTCTTAGATA TGGCACCTAA AGGAGACAGG 
AAGTGGCTTC ATGGATTACA GGCAGCCCTC CAGAACTTTC TCAAGGGATA TCCTGCAATT 
GTTCGGCAAC TGCACTCAGC AGGCAGAGGC AAAAGTGACA CCAGTCAAAA GAAACCCAAA 
GAACTTCTGG AGTCCCTTCT CCAAGCTGAC ATTGTTAAAT TTGCCCACTT CTTGTTGGAT 
GTCATGAATG TCCTTAGCAT TCTGTCCTGT GTCAATAAGA ATAGAAATTC CTCAATTGCT 
GACATATTTG CCACTTTAGA GTCAATGCTG GAAATGCTCC AAATGTATCA AACAAGACTA 
GGGCCAAAAG AACGCATGGT GGATTCAGCC ACACACTTTC ATGGTAACTG CCTCAGAGGG 
AAAGAAAACA TTTCTGCTGT GAGAAACATT GTTTTAACAC ATCTTATCAA GAGACTTCAA 
GGCTGTTTCA GAGATGCCAG CCAAAATGTG GTGAGGGCTA CCGTGATTGG AAGTTTTAAA 
TTGTGGCCCA CAAAGATAAA TCAAGAATTT GGAGAAAAAG AGGTATCCAT CCTGACTGCA 
CATTATGAAC CAGTATTAGA AGCTGCCAAT GTGAAACTTA GTGAAGTGGA TACTGAATGG 
AGCATGTTGA AATTAGAGAT TTATGCCAGA TTTCAGAACA TTCGAAAATT GACCTGGGAT 
TTTGTGAATT CCATTTACTT ACACAAGTAT CCAAATATCC TGACACTAGT CGACCTAGTG 
TTGACCCTTC CTGCAAGTTC AGCTGAAGCA GAACATGGCT TTAGTCAGAT GAAATGGACC 
AAGTCACATA TGCATGCAAA AATCAAGGCT GAAAGCATAA TAGGAAATAA AGCATTTACT 
GACATTCTGA TCGATTTGGT GACCCTGAAC CTTGGCACAA ACAACTTTCC TAGTAATTGG 
ATGCACCTTA CCCAACCTGA ATTACAGGAA TTAACATCTT TATTAACAGT CAACTCCGAG 
TTCGTGGACT GGCGGAAGTT CCTGTTAGTA ACCTCAATGC CTTGGCCCAT TCCCTTGGAG 
GAGGAGCTCC TTGAGACCCT TCAGAAGTTC AAGGCTGTGG ATAAGGAGCA GTTGGGCACC 
ATCACTTTTG AGCAGTATAT GCAGGCTGGT CTGTGGTTTA CAGGAGATGA AGATATAAAA 
ATTCCAGAAA ATCCTCTTGA ACCCCTTCCA TTTAATAGGC AGGAGCATCT TATAGAGTTT 
TTCTTTAGGC TATTTGCTGA CTATGAGAAG GATCCACCCC AGCTTGACTA CACACAGATG 
CTGCTTTACT TTGCTTGCCA CCCAGACACC GTGGAAGGAG TCTACAGGGC CCTCAGTGTG 
GCTGTTGGAA CTCATGTCTT CCAACAAGTC AAAGCTTCCA TTCCAAGTGC AGAAAAGACC 
TCCTCAACTG ATGCAGGTCC AGCTGAGGAA TTTCCTGAAC CTGAGGAAAA TGCTGCAAGA 
GAAGAAAGGA AATTAAAAGA CGACACGGAG AAAAGGGAAC AGAAGGATGA AGAAATCCCT 
GAAAATGCAA ACAATGAAAA GATGTCCATG GAAACACTAC TCAAAGTGTT CAAAGGGGGA 
AGTGAAGCAC AGGACTCCAA TAGATTTGCC AGCCACCTAA AGATAGAGAA CATTTATGCA 
GAGGGCTTCA TAAAAACATT TCAAGACCTA GGTGCCAAGA ACCTGGAGCC AATTGAAGTC 
GCTGTTCTCT TGAAGCATCC TTTTATTCAA GACCTGATTT CAAATTATTC AGACTATAAG 
TTTCCTGTAC ACATCATCTA TGTAGTGGAA ATGATAGTGT GTGCAAAAGG TTGGGCACCA 
CTTCCATCAG GAAAACAGTG TGCAAGATGG AGAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_011514140.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011512442.1 Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

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CloneID	OHu72408
Clone ID Related Accession (Same CDS sequence)	XM_011514140.2
Accession Version	XM_011514140.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sperm flagellar protein 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011514140.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG 
CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG 
GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA 
TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT 
GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA 
CCAGTTCCTG AGGAAATGCC TTTGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC 
TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGACCAGCA TACTGTGCTT 
GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC GGATCACAAG 
CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT 
GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG 
GACATTTGTG ATGCCCGGAA GGAAGAGGCG GAGCAGGAGC GGCTTGACAT CATTAATGAG 
AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA 
GAGCTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA 
AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCGAA TCCCTTTGGT CCAACTGGAT 
AGTAAAGACA ATTCTGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT 
CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGGGCAA GATGGATAAC 
TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG 
CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG 
GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA 
AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT 
AAATCACCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA 
ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA 
TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT 
CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT 
GAGAGGTATT TGAATGAAAT GGCCAGTACA GAAAAATTAA CTGACGTAGC TCGCTATCAC 
ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT 
AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCATCAA TACGTCCTCC ACCTGTAGAA 
AAGGAAGAAG ATGGTACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA 
GATATGGCAC CTAAAGGAGA CAGGAAGTGG CTTCATGGAT TACAGGCAGC CCTCCAGAAC 
TTTCTCAAGG GATATCCTGC AATTGTTCGG CAACTGCACT CAGCAGGCAG AGGCAAAAGT 
GACACCAGTC AAAAGAAACC CAAAGAACTT CTGGAGTCCC TTCTCCAAGC TGACATTGTT 
AAATTTGCCC ACTTCTTGTT GGATGTCATG AATGTCCTTA GCATTCTGTC CTGTGTCAAT 
AAGAATAGAA ATTCCTCAAT TGCTGACATA TTTGCCACTT TAGAGTCAAT GCTGGAAATG 
CTCCAAATGT ATCAAACAAG ACTAGGGCCA AAAGAACGCA TGGTGGATTC AGCCACACAC 
TTTCATGGTA ACTGCCTCAG AGGGAAAGAA AACATTTCTG CTGTGAGAAA CATTGTTTTA 
ACACATCTTA TCAAGAGACT TCAAGGCTGT TTCAGAGATG CCAGCCAAAA TGTGGTGAGG 
GCTACCGTGA TTGGAAGTTT TAAATTGTGG CCCACAAAGA TAAATCAAGA ATTTGGAGAA 
AAAGAGGTAT CCATCCTGAC TGCACATTAT GAACCAGTAT TAGAAGCTGC CAATGTGAAA 
CTTAGTGAAG TGGATACTGA ATGGAGCATG TTGAAATTAG AGATTTATGC CAGATTTCAG 
AACATTCGAA AATTGACCTG GGATTTTGTG AATTCCATTT ACTTACACAA GTATCCAAAT 
ATCCTGACAC TAGTCGACCT AGTGTTGACC CTTCCTGCAA GTTCAGCTGA AGCAGAACAT 
GGCTTTAGTC AGATGAAATG GACCAAGTCA CATATGCATG CAAAAATCAA GGCTGAAAGC 
ATAATAGGAA ATAAAGCATT TACTGACATT CTGATCGATT TGGTGACCCT GAACCTTGGC 
ACAAACAACT TTCCTAGTAA TTGGATGCAC CTTACCCAAC CTGAATTACA GGAATTAACA 
TCTTTATTAA CAGTCAACTC CGAGTTCGTG GACTGGCGGA AGTTCCTGTT AGTAACCTCA 
ATGCCTTGGC CCATTCCCTT GGAGGAGGAG CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT 
GTGGATAAGG AGCAGTTGGG CACCATCACT TTTGAGCAGT ATATGCAGGC TGGTCTGTGG 
TTTACAGGAG ATGAAGATAT AAAAATTCCA GAAAATCCTC TTGAACCCCT TCCATTTAAT 
AGGCAGGAGC ATCTTATAGA GTTTTTCTTT AGGCTATTTG CTGACTATGA GAAGGATCCA 
CCCCAGCTTG ACTACACACA GATGCTGCTT TACTTTGCTT GCCACCCAGA CACCGTGGAA 
GGAGTCTACA GGGCCCTCAG TGTGGCTGTT GGAACTCATG TCTTCCAACA AGTCAAAGCT 
TCCATTCCAA GTGCAGAAAA GACCTCCTCA ACTGATGCAG GTCCAGCTGA GGAATTTCCT 
GAACCTGAGG AAAATGCTGC AAGAGAAGAA AGGAAATTAA AAGACGACAC GGAGAAAAGG 
GAACAGAAGG ATGAAGAAAT CCCTGAAAAT GCAAACAATG AAAAGATGTC CATGGAAACA 
CTACTCAAAG TGTTCAAAGG GGGAAGTGAA GCACAGGACT CCAATAGATT TGCCAGCCAC 
CTAAAGATAG AGAACATTTA TGCAGAGGGC TTCATAAAAA CATTTCAAGA CCTAGGTGCC 
AAGAACCTGG AGCCAATTGA AGTCGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG 
ATTTCAAATT ATTCAGACTA TAAGTTTCCT GTACACATCA TCTATGTAGT GGAAATGATA 
GTGTGTGCAA AAGGTTGGGC ACCACTTCCA TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011512442.1
CDS	127..6249
Translation	MSEILCQWLNKELKVSRTVSPKSFAKAFSSGYLLGEVLHKFELQDDFSEFLDSRVSSAKLNNFSRLEPTLNLLGVQFDQNVAHGIITEKPGVATKLLYQLYIALQKKKKSGLTGVEMQTMQRLTNLRLQNMKSDTFQEQYLNRRRQNEIMAKIQAAIIQIPKPASNRTLKALEAQKMMKKKKEAEDVADEIKKFEALIKKDLQAKESASKTSLDTAGQTTTDLLNTYSDDEYIKKIQKRLEEDAFAREQREKRRRKLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKEVLWQNRIFREKQHEERRLKDFQDALDREAALAKQAKIDFEEQFLKEKRFHDQIAVERAQARYEKHYSVCAEILDQIVDLSTKVADYRMLTNNLIPYKLMHDWKELFFNAKPIYEQASVKTLPANPSREQLTELEKRDLLDTNDYEEYKNMVGEWALPEEMVDNLPPSNNCILGHILHRLAEKSLPPRAESTTPELPSFAVKGCLLGKTLSGKTTILRSLQKDFPIQILSIDTLVQEAIQAFHDNEKVSEVLPIQKNDEEDALPVLQEEIKESQDPQHVFSAGPVSDEVLPETEGETMLSANADKTPKAEEVKSSDSFLKLTTRAQLGAKSEQLLKKGKSIPDVLLVDIIVNAINEIPVNQDCILDGFPMTLNQAQLLEEALTGCNRNLTEVERKKAQKSTLAIDPATSKEIPLPSPAFDFVILLDVSDTSSMSRMNDIIAEELSYKTAHEDISQRVAAENQDKDGDQNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIKINAEIDKESLCEKVKEILTTEIAKKKNKVEKKLEEKEAEKKAAASLAELPLPTPPPAPPPEPEKEKEIHQSHVASKTPTAKGKPQSEAPHGKQESLQEGKGKKGETALKRKGSPKGKSSGGKVPVKKSPADSTDTSPVAIVPQPPKPGSEEWVYVNEPVPEEMPLFLVPYWELIENSYINTIKTVLRHLREDQHTVLAYLYEIRTSFQEFLKRPDHKQDFVAQWQADFNSLPDDLWDDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDTLGMTMNHFFSLMQAELNRFQDTKRLLQDYYWGMESKIPVEDNKRFTRIPLVQLDSKDNSESQLRIPLVPRISISLETVTPKPKTKSVLKGKMDNSLENVESNFEADEKLVMDTWQQASLAVSHMVAAEIHQRLMEEEKENQPADPKEKSPQMGANKKVKKEPPKKKQEDKKPKGKSPPMAEATPVIVTTEEIAEIKRKNELRVKIKEEHLAALQFEEIATQFRLELIKTKALALLEDLVTKVVDVYKLMEKWLGERYLNEMASTEKLTDVARYHIETSTKIQNELYLSQEDFFINGNIKVFPDPPPSIRPPPVEKEEDGTLTIEQLDSLRDQFLDMAPKGDRKWLHGLQAALQNFLKGYPAIVRQLHSAGRGKSDTSQKKPKELLESLLQADIVKFAHFLLDVMNVLSILSCVNKNRNSSIADIFATLESMLEMLQMYQTRLGPKERMVDSATHFHGNCLRGKENISAVRNIVLTHLIKRLQGCFRDASQNVVRATVIGSFKLWPTKINQEFGEKEVSILTAHYEPVLEAANVKLSEVDTEWSMLKLEIYARFQNIRKLTWDFVNSIYLHKYPNILTLVDLVLTLPASSAEAEHGFSQMKWTKSHMHAKIKAESIIGNKAFTDILIDLVTLNLGTNNFPSNWMHLTQPELQELTSLLTVNSEFVDWRKFLLVTSMPWPIPLEEELLETLQKFKAVDKEQLGTITFEQYMQAGLWFTGDEDIKIPENPLEPLPFNRQEHLIEFFFRLFADYEKDPPQLDYTQMLLYFACHPDTVEGVYRALSVAVGTHVFQQVKASIPSAEKTSSTDAGPAEEFPEPEENAAREERKLKDDTEKREQKDEEIPENANNEKMSMETLLKVFKGGSEAQDSNRFASHLKIENIYAEGFIKTFQDLGAKNLEPIEVAVLLKHPFIQDLISNYSDYKFPVHIIYVVEMIVCAKGWAPLPSGKQCARWRN

Target ORF information:

RefSeq Version	XM_011514140.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm flagellar 2 (SPEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011514140.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC AAGGAGTTGA AGGTGTCCCG GACCGTGAGT 
CCCAAGTCAT TTGCAAAGGC ATTTTCCAGT GGCTATCTAC TTGGAGAAGT TCTACACAAG 
TTTGAACTTC AGGATGATTT TTCAGAATTT TTGGACAGCA GGGTTTCAAG TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTT AACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCATG GCATCATCAC AGAAAAGCCT GGGGTGGCAA CAAAGCTGTT ATATCAATTG 
TACATTGCTC TTCAGAAAAA GAAGAAAAGT GGACTGACTG GAGTGGAGAT GCAAACCATG 
CAACGTCTGA CAAATTTAAG ACTTCAAAAC ATGAAAAGTG ATACTTTTCA AGAGCAATAC 
TTAAACAGAA GACGACAAAA TGAAATAATG GCCAAAATCC AAGCAGCTAT TATACAGATT 
CCTAAACCTG CATCAAATCG TACTTTGAAA GCACTCGAGG CCCAAAAAAT GATGAAAAAG 
AAAAAAGAGG CAGAAGATGT GGCTGATGAA ATTAAGAAGT TCGAAGCATT AATAAAAAAG 
GATCTCCAAG CAAAAGAAAG TGCATCCAAG ACTTCTTTAG ATACAGCAGG CCAGACAACC 
ACCGATTTGT TAAATACTTA CTCAGATGAC GAGTACATTA AAAAAATCCA GAAGCGCCTT 
GAAGAAGATG CTTTTGCACG AGAGCAAAGG GAGAAAAGAC GGCGGAAATT GTTAATGGAC 
CAGTTAATAG CCCACGAAGC ACAAGAGGAG GCTTATCGGG AGGAACAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATTGCCGTGC AGCTCATGCA TGTTCGGCAT 
GAAAAGGAAG TTTTATGGCA AAACAGAATT TTCAGAGAAA AACAACATGA GGAAAGACGA 
CTTAAAGATT TCCAGGATGC TCTTGATCGA GAAGCGGCTT TGGCAAAACA AGCCAAGATT 
GACTTTGAAG AACAATTCCT TAAAGAAAAG AGATTTCATG ATCAGATTGC TGTGGAAAGA 
GCTCAAGCTC GTTATGAAAA GCATTATTCA GTATGTGCAG AAATTTTGGA TCAAATAGTT 
GATTTGTCCA CTAAAGTGGC AGACTATCGA ATGTTGACAA ATAATCTGAT TCCGTATAAG 
TTGATGCATG ATTGGAAGGA ACTATTTTTT AATGCAAAAC CCATATATGA ACAAGCCTCT 
GTTAAGACAC TACCTGCTAA CCCCTCAAGA GAACAACTTA CAGAACTGGA GAAAAGGGAC 
TTGCTAGATA CCAATGATTA TGAAGAATAT AAGAACATGG TTGGAGAGTG GGCCTTACCA 
GAAGAAATGG TTGACAATTT ACCACCCTCC AACAATTGCA TACTGGGCCA TATTCTTCAC 
AGGCTAGCTG AAAAATCTCT TCCTCCTCGA GCGGAATCAA CAACACCTGA ATTACCTTCA 
TTTGCTGTTA AAGGATGCTT ATTGGGGAAA ACATTAAGTG GAAAAACTAC CATTTTAAGG 
TCTCTACAAA AAGACTTTCC TATACAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT TTCATGACAA TGAAAAAGTC AGTGAGGTTC TACCAATTCA GAAAAATGAT 
GAAGAAGATG CTCTACCAGT TCTGCAAGAG GAGATTAAAG AAAGCCAGGA TCCACAACAT 
GTATTTTCAG CTGGTCCAGT TTCAGATGAA GTATTACCAG AAACAGAAGG TGAAACAATG 
CTTAGTGCTA ATGCTGATAA AACACCAAAA GCTGAAGAAG TCAAATCAAG TGATAGTTTC 
TTAAAACTCA CTACACGTGC TCAGCTTGGT GCAAAATCAG AACAGTTGCT GAAGAAAGGA 
AAGAGCATTC CTGATGTGCT GCTTGTTGAC ATCATAGTAA ATGCTATTAA TGAGATACCT 
GTGAATCAAG ACTGTATCCT AGATGGTTTT CCAATGACTT TAAACCAAGC ACAGCTTCTG 
GAAGAAGCTC TTACAGGCTG CAATAGAAAC CTCACAGAAG TGGAAAGAAA AAAAGCACAA 
AAATCCACAT TGGCTATTGA TCCTGCGACT TCCAAAGAAA TACCTCTTCC CTCTCCTGCA 
TTTGATTTTG TCATATTATT AGATGTTTCA GATACTTCCT CAATGAGTCG CATGAATGAT 
ATTATAGCTG AAGAATTGTC CTATAAAACT GCTCACGAAG ATATCAGTCA ACGTGTAGCT 
GCTGAAAACC AAGATAAGGA TGGAGACCAA AATTTAAGAG ACCAGATACA ACATAGAATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAAAA TCAATGCTGA AATAGATAAG GAATCTTTAT GTGAAAAAGT AAAAGAAATT 
CTTACGACTG AAATAGCAAA AAAAAAGAAT AAAGTTGAGA AGAAATTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCAGC TTCCCTGGCT GAGCTTCCAC TTCCTACACC TCCTCCTGCT 
CCTCCTCCTG AACCAGAAAA AGAGAAGGAA ATTCATCAAA GCCATGTGGC TTCAAAAACT 
CCTACTGCAA AAGGAAAACC TCAATCAGAA GCCCCGCATG GTAAGCAAGA ATCTCTTCAG 
GAAGGAAAAG GGAAGAAAGG TGAGACCGCA CTCAAAAGAA AAGGTTCTCC TAAAGGAAAA 
TCATCAGGAG GAAAAGTACC AGTAAAGAAA TCACCTGCTG ACTCTACAGA TACATCACCT 
GTTGCAATAG TGCCACAGCC ACCTAAGCCA GGATCAGAAG AATGGGTCTA TGTGAATGAA 
CCAGTTCCTG AGGAAATGCC TTTGTTTTTA GTACCTTACT GGGAACTAAT AGAAAATTCC 
TATATAAACA CCATCAAAAC AGTACTCAGG CATCTGAGGG AAGACCAGCA TACTGTGCTT 
GCTTACTTAT ATGAGATTAG AACAAGTTTC CAGGAGTTTC TAAAGCGTCC GGATCACAAG 
CAAGATTTTG TAGCTCAATG GCAGGCTGAT TTCAACTCCC TTCCTGATGA CCTGTGGGAT 
GATGAGGAAA CAAAGGCTGA ACTACATCAA CGAGTGAATG ATCTGCGAGA CCGCCTGTGG 
GACATTTGTG ATGCCCGGAA GGAAGAGGCG GAGCAGGAGC GGCTTGACAT CATTAATGAG 
AGCTGGTTAC AGGACACTCT TGGAATGACA ATGAACCATT TCTTTTCCCT GATGCAGGCA 
GAGCTGAACC GTTTCCAAGA TACAAAGAGA CTCCTTCAAG ATTATTACTG GGGAATGGAA 
AGTAAAATCC CAGTAGAGGA CAACAAGAGA TTTACTCGAA TCCCTTTGGT CCAACTGGAT 
AGTAAAGACA ATTCTGAAAG CCAGCTTAGA ATTCCTCTAG TTCCTCGAAT ATCCATTTCT 
CTGGAAACAG TTACACCCAA ACCAAAAACA AAATCAGTAC TGAAGGGCAA GATGGATAAC 
TCCCTAGAAA ACGTTGAGTC CAACTTTGAG GCCGATGAAA AGTTGGTCAT GGACACCTGG 
CAGCAGGCTT CTTTAGCAGT ATCTCACATG GTGGCTGCTG AAATTCATCA GAGGCTTATG 
GAAGAAGAAA AAGAAAACCA GCCAGCAGAC CCCAAAGAAA AATCTCCTCA GATGGGTGCA 
AATAAAAAAG TCAAAAAGGA GCCACCCAAG AAAAAACAGG AAGACAAAAA ACCCAAAGGT 
AAATCACCAC CTATGGCAGA AGCAACTCCT GTCATAGTAA CAACAGAGGA AATTGCTGAA 
ATCAAAAGGA AAAATGAACT GAGGGTCAAA ATAAAAGAAG AACACCTTGC TGCCTTGCAA 
TTTGAAGAAA TAGCCACGCA ATTTCGACTT GAACTGATAA AGACAAAAGC ATTGGCTCTT 
CTTGAAGATT TAGTAACAAA GGTGGTTGAT GTATATAAAC TCATGGAAAA ATGGCTTGGT 
GAGAGGTATT TGAATGAAAT GGCCAGTACA GAAAAATTAA CTGACGTAGC TCGCTATCAC 
ATTGAAACAT CTACAAAAAT TCAGAATGAA CTTTATTTAA GCCAAGAAGA CTTCTTCATT 
AATGGCAATA TAAAAGTCTT CCCAGATCCT CCCCCATCAA TACGTCCTCC ACCTGTAGAA 
AAGGAAGAAG ATGGTACCCT GACCATTGAA CAGCTTGACA GTCTTCGAGA TCAGTTCTTA 
GATATGGCAC CTAAAGGAGA CAGGAAGTGG CTTCATGGAT TACAGGCAGC CCTCCAGAAC 
TTTCTCAAGG GATATCCTGC AATTGTTCGG CAACTGCACT CAGCAGGCAG AGGCAAAAGT 
GACACCAGTC AAAAGAAACC CAAAGAACTT CTGGAGTCCC TTCTCCAAGC TGACATTGTT 
AAATTTGCCC ACTTCTTGTT GGATGTCATG AATGTCCTTA GCATTCTGTC CTGTGTCAAT 
AAGAATAGAA ATTCCTCAAT TGCTGACATA TTTGCCACTT TAGAGTCAAT GCTGGAAATG 
CTCCAAATGT ATCAAACAAG ACTAGGGCCA AAAGAACGCA TGGTGGATTC AGCCACACAC 
TTTCATGGTA ACTGCCTCAG AGGGAAAGAA AACATTTCTG CTGTGAGAAA CATTGTTTTA 
ACACATCTTA TCAAGAGACT TCAAGGCTGT TTCAGAGATG CCAGCCAAAA TGTGGTGAGG 
GCTACCGTGA TTGGAAGTTT TAAATTGTGG CCCACAAAGA TAAATCAAGA ATTTGGAGAA 
AAAGAGGTAT CCATCCTGAC TGCACATTAT GAACCAGTAT TAGAAGCTGC CAATGTGAAA 
CTTAGTGAAG TGGATACTGA ATGGAGCATG TTGAAATTAG AGATTTATGC CAGATTTCAG 
AACATTCGAA AATTGACCTG GGATTTTGTG AATTCCATTT ACTTACACAA GTATCCAAAT 
ATCCTGACAC TAGTCGACCT AGTGTTGACC CTTCCTGCAA GTTCAGCTGA AGCAGAACAT 
GGCTTTAGTC AGATGAAATG GACCAAGTCA CATATGCATG CAAAAATCAA GGCTGAAAGC 
ATAATAGGAA ATAAAGCATT TACTGACATT CTGATCGATT TGGTGACCCT GAACCTTGGC 
ACAAACAACT TTCCTAGTAA TTGGATGCAC CTTACCCAAC CTGAATTACA GGAATTAACA 
TCTTTATTAA CAGTCAACTC CGAGTTCGTG GACTGGCGGA AGTTCCTGTT AGTAACCTCA 
ATGCCTTGGC CCATTCCCTT GGAGGAGGAG CTCCTTGAGA CCCTTCAGAA GTTCAAGGCT 
GTGGATAAGG AGCAGTTGGG CACCATCACT TTTGAGCAGT ATATGCAGGC TGGTCTGTGG 
TTTACAGGAG ATGAAGATAT AAAAATTCCA GAAAATCCTC TTGAACCCCT TCCATTTAAT 
AGGCAGGAGC ATCTTATAGA GTTTTTCTTT AGGCTATTTG CTGACTATGA GAAGGATCCA 
CCCCAGCTTG ACTACACACA GATGCTGCTT TACTTTGCTT GCCACCCAGA CACCGTGGAA 
GGAGTCTACA GGGCCCTCAG TGTGGCTGTT GGAACTCATG TCTTCCAACA AGTCAAAGCT 
TCCATTCCAA GTGCAGAAAA GACCTCCTCA ACTGATGCAG GTCCAGCTGA GGAATTTCCT 
GAACCTGAGG AAAATGCTGC AAGAGAAGAA AGGAAATTAA AAGACGACAC GGAGAAAAGG 
GAACAGAAGG ATGAAGAAAT CCCTGAAAAT GCAAACAATG AAAAGATGTC CATGGAAACA 
CTACTCAAAG TGTTCAAAGG GGGAAGTGAA GCACAGGACT CCAATAGATT TGCCAGCCAC 
CTAAAGATAG AGAACATTTA TGCAGAGGGC TTCATAAAAA CATTTCAAGA CCTAGGTGCC 
AAGAACCTGG AGCCAATTGA AGTCGCTGTT CTCTTGAAGC ATCCTTTTAT TCAAGACCTG 
ATTTCAAATT ATTCAGACTA TAAGTTTCCT GTACACATCA TCTATGTAGT GGAAATGATA 
GTGTGTGCAA AAGGTTGGGC ACCACTTCCA TCAGGAAAAC AGTGTGCAAG ATGGAGAAAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPEF2
Entrez Gene ID	79925
Full Name	sperm flagellar 2
Synonyms	CT122,KPL2
General protein information	Preferred Names sperm flagellar 2 Names sperm flagellar protein 2 cancer/testis antigen 122 testis tissue sperm-binding protein Li 47a
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p13.2
Disorder MIM:	610172

Canis lupus familiaris (dog)	SPEF2	XP_546353.2
Gallus gallus (chicken)	SPEF2	XP_003643024.2
Danio rerio (zebrafish)	spef2	NP_001121704.1
Homo sapiens (human)	SPEF2	NP_079143.3
	LOC703498	XP_001091802.1
Mus musculus (house mouse)	Spef2	NP_796097.2
Pan troglodytes (chimpanzee)	SPEF2	XP_001143539.2
Bos taurus (cattle)	SPEF2	XP_002696427.2
Rattus norvegicus (Norway rat)	Spef2	NP_072142.1
Xenopus tropicalis (tropical clawed frog)	spef2	XP_002940003.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPEF2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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