TOP2B cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2B
Entrez Gene ID	460232
Full Name	DNA topoisomerase II beta
General protein information	Preferred Names DNA topoisomerase II beta Names DNA topoisomerase 2-beta DNA topoisomerase II, beta isozyme topoisomerase (DNA) II beta 180kDa
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009445032.2	XP_009443307.2	DNA topoisomerase 2-beta isoform X1
XM_009445032.3	XP_009443307.2	DNA topoisomerase 2-beta isoform X1
XM_016940585.2	XP_016796074.2	DNA topoisomerase 2-beta isoform X2
XM_516332.5	XP_516332.4	DNA topoisomerase 2-beta isoform X2
XM_016940585.1	XP_016796074.1	DNA topoisomerase 2-beta isoform X3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology

The following TOP2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc81500	XM_009445032.3 Latest version!	Pan troglodytes DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc81501	XM_016940585.2 Latest version!	Pan troglodytes DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc81501	XM_516332.5 Latest version!	Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc81502	XM_016940585.1 Latest version!	Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OPc81500	XM_009445032.2	Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2B ( XM_009445032.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009443307.2 Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc81500
Clone ID Related Accession (Same CDS sequence)	XM_009445032.2 , XM_009445032.3
Accession Version	XM_009445032.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	DNA topoisomerase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009445032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA CTCTTTTTGA TCAGAACAAT GCTGCAAAAA AAGAAGAGTC AGAAACTGCC 
AACAAAAATG ATTCTTCAAA GAAGTTGTCT GTTGAGAGAG TGTATCAGAA GAAGACACAA 
CTTGAACACA TTCTTCTTCG TCCTGATACA TATATTGGGT CAGTGGAGCC ATTGACGCAG 
TTCATGTGGG TGTATGATGA AGATGTAGGA ATGAATTGCA GAGAGGTTAC CTTTGTGCCA 
GGTTTATACA AGATCTTTGA TGAAATTTTG GTTAATGCTG CTGACAATAA ACAGAGGGAT 
AAGAACATGA CTTGTATTAA AGTTTCTATT GATCCTGAAT CTAACATTAT AAGCATTTGG 
AATAATGGGA AAGGCATTCC AGTAGTAGAA CACAAGGTAG AGAAAGTTTA TGTTCCTGCT 
TTAATTTTTG GACAGCTTTT AACATCCAGT AACTATGATG ATGATGAGAA AAAAGTTACA 
GGTGGTCGTA ATGGTTATGG TGCAAAACTT TGTAATATTT TCAGTACAAA GTTTACAGTA 
GAAACAGCTT GCAAAGAATA CAAACACAGT TTTAAGCAGA CATGGATGAA TAATATGATG 
AAGACTTCTG AAGCCAAAAT TAAACATTTT GATGGTGAAG ATTACACATG CATAACATTC 
CAACCAGATC TGTCCAAATT TAAGATGGAA AAACTTGACA AGGATATTGT GGCCCTCATG 
ACTAGAAGGG CATATGATTT GGCTGGTTCG TGTAGAGGGG TCAAGGTCAT GTTTAATGGA 
AAGAAATTGC CTGTAAATGG ATTTCGCAGT TATGTAGATC TTTATGTGAA AGACAAATTG 
GATGAAACTG GGGTGGCCCT GAAAGTTATT CATGAGCTTG CAAATGAAAG ATGGGATGTT 
TGTCTCACAT TGAGTGAAAA AGGATTCCAG CAAATCAGTT TTGTAAATAG TATTGCAACT 
ACAAAAGGTG GACGGCACGT GGATTATGTG GTAGATCAAG TTGTTGGTAA ACTGATTGAA 
GTAGTTAAGA AAAAGAACAA AGCTGGTGTA TCAGTGAAAC CATTTCAAGT AAAAAACCAT 
ATATGGGTTT TTATTAATTG CCTTATTGAA AATCCAACTT TTGATTCTCA GACTAAGGAA 
AACATGACTC TGCAGCCCAA AAGTTTTGGG TCTAAATGCC AGCTGTCAGA AAAATTTTTT 
AAAGCAGCCT CTAATTGTGG CATTGTAGAA AGTATCCTGA ACTGGGTGAA ATTTAAGGCT 
CAGACTCAGC TGAATAAGAA GTGTTCATCA GTAAAATACA GTAAAATCAA AGGTATTCCC 
AAACTGGATG ATGCTAATGA TGCTGGTGGT AAACATTCCC TGGAGTGTAC ACTGATATTA 
ACAGAGGGAG ACTCTGCCAA ATCACTGGCT GTGTCTGGAT TAGGTGTGAT TGGACGAGAC 
AGATATGGAG TTTTTCCACT CAGGGGCAAA ATTCTTAATG TACGGGAAGC TTCTCATAAA 
CAGATCATGG AAAATGCTGA AATAAATAAT ATTATTAAAA TAGTTGGTCT ACAATATAAG 
AAAAGTTACG ATGATGCAGA ATCTCTGAAA ACCTTACGCT ATGGAAAGAT TATGATTATG 
ACCGATCAGG ATCAAGATGG TTCTCACATA AAAGGCCTGC TTATTAATTT CATCCATCAC 
AATTGGCCAT CACTTTTGAA GCATGGTTTT CTCGAAGAGT TCATTACTCC TATTGTAAAG 
GCAAGCAAAA ATAAGCAGGA ACTTTCCTTC TACAGTATTC CTGAATTTGA CGAATGGAAA 
AAACATATAG AAAACCAGAA AGCCTGGAAA ATAAAGTACT ATAAAGGATT GGGTACTAGT 
ACAGCTAAAG AAGCAAAGGA ATATTTTGCT GATATGGAAA GGCATCGCAT CTTGTTTAGA 
TATGCTGGTC CTGAAGATGA TGCTGCCATT ACCTTGGCAT TTAGTAAGAA GAAGATTGAT 
GACAGAAAAG AATGGTTAAC AAATTTTATG GAAGACCGGA GACAGCGTAG GCTACATGGC 
TTACCAGAGC AATTTTTATA TGGTACTGCA ACAAAGCATT TGACTTATAA TGATTTCATC 
AACAAGGAAT TGATTCTCTT CTCAAACTCA GACAATGAAA GATCTATACC ATCTCTTGTT 
GATGGCTTTA AACCTGGCCA GCGGAAAGTT TTATTTACCT GTTTCAAGAG GAATGATAAA 
CGTGAAGTAA AAGTTGCCCA GTTGGCTGGC TCTGTTGCTG AGATGTCGGC TTATCATCAT 
GGAGAACAAG CATTGATGAT GACTATTGTG AATTTGGCTC AGAACTTTGT GGGAAGTAAC 
AACATTAACT TGCTTCAGCC TATTGGTCAG TTTGGAACTC GGCTTCATGG TGGCAAAGAT 
GCTGCAAGCC CTCGTTATAT TTTCACAATG TTAAGCACTT TAGCAAGGCT ACTTTTTCCT 
GCTGTGGATG ACAACCTCCT TAAGTTCCTT TATGATGATA ATCAACGTGT AGAGCCTGAG 
TGGTATATTC CTATAATTCC CATGGTTTTA ATAAATGGTG CTGAGGGCAT TGGTACTGGA 
TGGGCTTGTA AACTACCCAA CTATGATGCT AGGGAAATTG TGAACAATGT CAGACGAATG 
CTAGATGGCC TGGATCCTCA TCCCATGCTT CCAAACTACA AAAACTTTAA AGGCACGATT 
CAAGAACTTG GTCAAAACCA GTATGCAGTC AGTGGTGAAA TATTTGTAGT GGACAGAAAC 
ACAGTAGAAA TTACAGAGCT TCCAGTTAGA ACTTGGACAC AGGTATATAA AGAACAGGTT 
TTAGAACCTA TGCTAAATGG AACAGATAAA ACACCAGCAT TAATTTCTGA TTATAAAGAA 
TATCATACTG ACACAACTGT GAAATTTGTG GTGAAAATGA CTGAAGAGAA ACTAGCACAA 
GCAGAAGCTG CTGGACTGCA TAAAGTTTTT AAACTTCAAA CTACTCTTAC TTGTAATTCC 
ATGGTACTTT TTGATCATAT GGGATGTCTG AAGAAATATG AAACTGTGCA AGACATTCTG 
AAAGAATTCT TTGATTTACG ATTAAGTTAT TACGGTTTAC GTAAGGAGTG GCTTGTGGGA 
ATGTTGGGAG CAGAATCTAC AAAGCTTAAC AATCAAGCCC GTTTCATTTT AGAGAAGATA 
CAAGGGAAAA TTACTATAGA GAATAGGTCA AAGAAAGATT TGATTCAAAT GTTAGTCCAG 
AGAGGTTATG AATCTGACCC AGTGAAAGCC TGGAAAGAAG CACAAGAAAA GGCAGCAGAA 
GAGGATGAAA CACAAAACCA GCATGATGAT AGTTCCTCCG ACTCAGGAAC TCCTTCAGGC 
CCAGATTTTA ATTATATTTT AAATATGTCT CTGTGGTCTC TTACTAAAGA AAAAGTTGAA 
GAACTGATTA AACAGAGAGA TGCAAAAGGG CGAGAGGTCA ATGATCTTAA AAGAAAATCT 
CCTTCAGATC TTTGGAAAGA GGATTTAGCG GCATTTGTTG AAGAACTGGA TAAAGTGGAA 
TCTCAAGAAC GAGAAGATGT TCTGGCTGGA ATGTCTGGAA AAGCAATTAA AGGTAAAGTT 
GGCAAACCTA AGGTGAAGAA ACTCCAGTTG GAAGAGACAA TGCCCTCACC TTATGGCAGA 
AGAATAATTC CTGAAATTAC AGCTATGAAG GCAGATGCCA GCAAAAAGTT GCTGAAGAAG 
AAGAAGGGTG ATCTTGATAC TGCAGCAGTA AAAGTGGAAT TTGATGAAGA ATTCAGTGGA 
GCGCCAGTAG AAGGTGCAGG AGAAGAGGCA TTGACTCCAT CAGTTCCTAT AAATAAAGGC 
CCCAAACCCA AAAGGGAGAA GAAGGAGCCT GGTACCAGAG TGAGAAAAAC ACCTACATCA 
TCTGGTAAAC CTAGTGCAAA GAAAGTGAAG AAACGGAATC CTTGGTCAGA TGATGAATCC 
AAGTCAGAAA GTGATTTGGA AGAAACAGAA CCTGTGGTTA TTCCAAGAGA TTCTTTGCTT 
AGGAGAGCAG CAGCCGAAAG ACCTAAATAC ACATTTGATT TCTCAGAAGA AGAGGATGAT 
GATGCTGATG ATGATGATGA TGACAATAAT GATTTAGAGG AATTGAAAGT TAAAGCATCT 
CCCATAACAA ATGATGGGGA AGATGAATTT GTTCCTTCAG ATGGGTTAGA TAAAGATGAA 
TATACATTTT CACCAGGCAA ATCAAAAGCC ACTCCAGAAA AATCTTTGCA TGACAAAAAA 
AGTCAGGATT TTGGAAATCT CTTCTCATTT CCTTCATATT CTCAGAAGTC AGAAGATGAT 
TCAGCTAAAT TTGACAGTAA TGAAGAAGAT TCTGCTTCTG TTTTTTCACC ATCATTTGGT 
CTGAAACAGA CAGATAAAGT TCCAAGTAAA ACGGTAGCTG CTAAAAAGGG AAAACCGTCT 
TCAGATACAG TCCCTAAGCC CAAGAGAGCC CCAAAACAGA AGAAAGTAGT AGAGGCTGTA 
AACTCTGACT CGGATTCAGA ATTTGGCATT CCAAAGAAGA CTACAACACC AAAAGGTAAA 
GGCCGAGGGG CAAAGAAAAG GAAAGCATCT GGCTCTGAAA ATGAAGGCGA TTATAACCCT 
GGCAGGAAAA CATCCAAAAC AACAAGCAAG AAACCGAAGA AGACATCTTT TGATCAGGAT 
TCAGATGTGG ACATCTTCCC CTCAGACTTC CCTACTGAGC CACCTTCTCT GCCACGAACC 
GGTCGGGCTA GGAAAGAAGT AAAATATTTT GCAGAGTCTG ATGAAGAAGA TGATGATGTT 
GATTTTGCAA TGTTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009443307.2
CDS	638..5518
Translation	MAKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEDDDVDFAMFN

Target ORF information:

RefSeq Version	XM_009445032.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009445032.2

ORF Insert Sequence:

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA CTCTTTTTGA TCAGAACAAT GCTGCAAAAA AAGAAGAGTC AGAAACTGCC 
AACAAAAATG ATTCTTCAAA GAAGTTGTCT GTTGAGAGAG TGTATCAGAA GAAGACACAA 
CTTGAACACA TTCTTCTTCG TCCTGATACA TATATTGGGT CAGTGGAGCC ATTGACGCAG 
TTCATGTGGG TGTATGATGA AGATGTAGGA ATGAATTGCA GAGAGGTTAC CTTTGTGCCA 
GGTTTATACA AGATCTTTGA TGAAATTTTG GTTAATGCTG CTGACAATAA ACAGAGGGAT 
AAGAACATGA CTTGTATTAA AGTTTCTATT GATCCTGAAT CTAACATTAT AAGCATTTGG 
AATAATGGGA AAGGCATTCC AGTAGTAGAA CACAAGGTAG AGAAAGTTTA TGTTCCTGCT 
TTAATTTTTG GACAGCTTTT AACATCCAGT AACTATGATG ATGATGAGAA AAAAGTTACA 
GGTGGTCGTA ATGGTTATGG TGCAAAACTT TGTAATATTT TCAGTACAAA GTTTACAGTA 
GAAACAGCTT GCAAAGAATA CAAACACAGT TTTAAGCAGA CATGGATGAA TAATATGATG 
AAGACTTCTG AAGCCAAAAT TAAACATTTT GATGGTGAAG ATTACACATG CATAACATTC 
CAACCAGATC TGTCCAAATT TAAGATGGAA AAACTTGACA AGGATATTGT GGCCCTCATG 
ACTAGAAGGG CATATGATTT GGCTGGTTCG TGTAGAGGGG TCAAGGTCAT GTTTAATGGA 
AAGAAATTGC CTGTAAATGG ATTTCGCAGT TATGTAGATC TTTATGTGAA AGACAAATTG 
GATGAAACTG GGGTGGCCCT GAAAGTTATT CATGAGCTTG CAAATGAAAG ATGGGATGTT 
TGTCTCACAT TGAGTGAAAA AGGATTCCAG CAAATCAGTT TTGTAAATAG TATTGCAACT 
ACAAAAGGTG GACGGCACGT GGATTATGTG GTAGATCAAG TTGTTGGTAA ACTGATTGAA 
GTAGTTAAGA AAAAGAACAA AGCTGGTGTA TCAGTGAAAC CATTTCAAGT AAAAAACCAT 
ATATGGGTTT TTATTAATTG CCTTATTGAA AATCCAACTT TTGATTCTCA GACTAAGGAA 
AACATGACTC TGCAGCCCAA AAGTTTTGGG TCTAAATGCC AGCTGTCAGA AAAATTTTTT 
AAAGCAGCCT CTAATTGTGG CATTGTAGAA AGTATCCTGA ACTGGGTGAA ATTTAAGGCT 
CAGACTCAGC TGAATAAGAA GTGTTCATCA GTAAAATACA GTAAAATCAA AGGTATTCCC 
AAACTGGATG ATGCTAATGA TGCTGGTGGT AAACATTCCC TGGAGTGTAC ACTGATATTA 
ACAGAGGGAG ACTCTGCCAA ATCACTGGCT GTGTCTGGAT TAGGTGTGAT TGGACGAGAC 
AGATATGGAG TTTTTCCACT CAGGGGCAAA ATTCTTAATG TACGGGAAGC TTCTCATAAA 
CAGATCATGG AAAATGCTGA AATAAATAAT ATTATTAAAA TAGTTGGTCT ACAATATAAG 
AAAAGTTACG ATGATGCAGA ATCTCTGAAA ACCTTACGCT ATGGAAAGAT TATGATTATG 
ACCGATCAGG ATCAAGATGG TTCTCACATA AAAGGCCTGC TTATTAATTT CATCCATCAC 
AATTGGCCAT CACTTTTGAA GCATGGTTTT CTCGAAGAGT TCATTACTCC TATTGTAAAG 
GCAAGCAAAA ATAAGCAGGA ACTTTCCTTC TACAGTATTC CTGAATTTGA CGAATGGAAA 
AAACATATAG AAAACCAGAA AGCCTGGAAA ATAAAGTACT ATAAAGGATT GGGTACTAGT 
ACAGCTAAAG AAGCAAAGGA ATATTTTGCT GATATGGAAA GGCATCGCAT CTTGTTTAGA 
TATGCTGGTC CTGAAGATGA TGCTGCCATT ACCTTGGCAT TTAGTAAGAA GAAGATTGAT 
GACAGAAAAG AATGGTTAAC AAATTTTATG GAAGACCGGA GACAGCGTAG GCTACATGGC 
TTACCAGAGC AATTTTTATA TGGTACTGCA ACAAAGCATT TGACTTATAA TGATTTCATC 
AACAAGGAAT TGATTCTCTT CTCAAACTCA GACAATGAAA GATCTATACC ATCTCTTGTT 
GATGGCTTTA AACCTGGCCA GCGGAAAGTT TTATTTACCT GTTTCAAGAG GAATGATAAA 
CGTGAAGTAA AAGTTGCCCA GTTGGCTGGC TCTGTTGCTG AGATGTCGGC TTATCATCAT 
GGAGAACAAG CATTGATGAT GACTATTGTG AATTTGGCTC AGAACTTTGT GGGAAGTAAC 
AACATTAACT TGCTTCAGCC TATTGGTCAG TTTGGAACTC GGCTTCATGG TGGCAAAGAT 
GCTGCAAGCC CTCGTTATAT TTTCACAATG TTAAGCACTT TAGCAAGGCT ACTTTTTCCT 
GCTGTGGATG ACAACCTCCT TAAGTTCCTT TATGATGATA ATCAACGTGT AGAGCCTGAG 
TGGTATATTC CTATAATTCC CATGGTTTTA ATAAATGGTG CTGAGGGCAT TGGTACTGGA 
TGGGCTTGTA AACTACCCAA CTATGATGCT AGGGAAATTG TGAACAATGT CAGACGAATG 
CTAGATGGCC TGGATCCTCA TCCCATGCTT CCAAACTACA AAAACTTTAA AGGCACGATT 
CAAGAACTTG GTCAAAACCA GTATGCAGTC AGTGGTGAAA TATTTGTAGT GGACAGAAAC 
ACAGTAGAAA TTACAGAGCT TCCAGTTAGA ACTTGGACAC AGGTATATAA AGAACAGGTT 
TTAGAACCTA TGCTAAATGG AACAGATAAA ACACCAGCAT TAATTTCTGA TTATAAAGAA 
TATCATACTG ACACAACTGT GAAATTTGTG GTGAAAATGA CTGAAGAGAA ACTAGCACAA 
GCAGAAGCTG CTGGACTGCA TAAAGTTTTT AAACTTCAAA CTACTCTTAC TTGTAATTCC 
ATGGTACTTT TTGATCATAT GGGATGTCTG AAGAAATATG AAACTGTGCA AGACATTCTG 
AAAGAATTCT TTGATTTACG ATTAAGTTAT TACGGTTTAC GTAAGGAGTG GCTTGTGGGA 
ATGTTGGGAG CAGAATCTAC AAAGCTTAAC AATCAAGCCC GTTTCATTTT AGAGAAGATA 
CAAGGGAAAA TTACTATAGA GAATAGGTCA AAGAAAGATT TGATTCAAAT GTTAGTCCAG 
AGAGGTTATG AATCTGACCC AGTGAAAGCC TGGAAAGAAG CACAAGAAAA GGCAGCAGAA 
GAGGATGAAA CACAAAACCA GCATGATGAT AGTTCCTCCG ACTCAGGAAC TCCTTCAGGC 
CCAGATTTTA ATTATATTTT AAATATGTCT CTGTGGTCTC TTACTAAAGA AAAAGTTGAA 
GAACTGATTA AACAGAGAGA TGCAAAAGGG CGAGAGGTCA ATGATCTTAA AAGAAAATCT 
CCTTCAGATC TTTGGAAAGA GGATTTAGCG GCATTTGTTG AAGAACTGGA TAAAGTGGAA 
TCTCAAGAAC GAGAAGATGT TCTGGCTGGA ATGTCTGGAA AAGCAATTAA AGGTAAAGTT 
GGCAAACCTA AGGTGAAGAA ACTCCAGTTG GAAGAGACAA TGCCCTCACC TTATGGCAGA 
AGAATAATTC CTGAAATTAC AGCTATGAAG GCAGATGCCA GCAAAAAGTT GCTGAAGAAG 
AAGAAGGGTG ATCTTGATAC TGCAGCAGTA AAAGTGGAAT TTGATGAAGA ATTCAGTGGA 
GCGCCAGTAG AAGGTGCAGG AGAAGAGGCA TTGACTCCAT CAGTTCCTAT AAATAAAGGC 
CCCAAACCCA AAAGGGAGAA GAAGGAGCCT GGTACCAGAG TGAGAAAAAC ACCTACATCA 
TCTGGTAAAC CTAGTGCAAA GAAAGTGAAG AAACGGAATC CTTGGTCAGA TGATGAATCC 
AAGTCAGAAA GTGATTTGGA AGAAACAGAA CCTGTGGTTA TTCCAAGAGA TTCTTTGCTT 
AGGAGAGCAG CAGCCGAAAG ACCTAAATAC ACATTTGATT TCTCAGAAGA AGAGGATGAT 
GATGCTGATG ATGATGATGA TGACAATAAT GATTTAGAGG AATTGAAAGT TAAAGCATCT 
CCCATAACAA ATGATGGGGA AGATGAATTT GTTCCTTCAG ATGGGTTAGA TAAAGATGAA 
TATACATTTT CACCAGGCAA ATCAAAAGCC ACTCCAGAAA AATCTTTGCA TGACAAAAAA 
AGTCAGGATT TTGGAAATCT CTTCTCATTT CCTTCATATT CTCAGAAGTC AGAAGATGAT 
TCAGCTAAAT TTGACAGTAA TGAAGAAGAT TCTGCTTCTG TTTTTTCACC ATCATTTGGT 
CTGAAACAGA CAGATAAAGT TCCAAGTAAA ACGGTAGCTG CTAAAAAGGG AAAACCGTCT 
TCAGATACAG TCCCTAAGCC CAAGAGAGCC CCAAAACAGA AGAAAGTAGT AGAGGCTGTA 
AACTCTGACT CGGATTCAGA ATTTGGCATT CCAAAGAAGA CTACAACACC AAAAGGTAAA 
GGCCGAGGGG CAAAGAAAAG GAAAGCATCT GGCTCTGAAA ATGAAGGCGA TTATAACCCT 
GGCAGGAAAA CATCCAAAAC AACAAGCAAG AAACCGAAGA AGACATCTTT TGATCAGGAT 
TCAGATGTGG ACATCTTCCC CTCAGACTTC CCTACTGAGC CACCTTCTCT GCCACGAACC 
GGTCGGGCTA GGAAAGAAGT AAAATATTTT GCAGAGTCTG ATGAAGAAGA TGATGATGTT 
GATTTTGCAA TGTTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_009445032.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009443307.2 Pan troglodytes DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA.

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CloneID	OPc81500
Clone ID Related Accession (Same CDS sequence)	XM_009445032.2 , XM_009445032.3
Accession Version	XM_009445032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	DNA topoisomerase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009445032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA CTCTTTTTGA TCAGAACAAT GCTGCAAAAA AAGAAGAGTC AGAAACTGCC 
AACAAAAATG ATTCTTCAAA GAAGTTGTCT GTTGAGAGAG TGTATCAGAA GAAGACACAA 
CTTGAACACA TTCTTCTTCG TCCTGATACA TATATTGGGT CAGTGGAGCC ATTGACGCAG 
TTCATGTGGG TGTATGATGA AGATGTAGGA ATGAATTGCA GAGAGGTTAC CTTTGTGCCA 
GGTTTATACA AGATCTTTGA TGAAATTTTG GTTAATGCTG CTGACAATAA ACAGAGGGAT 
AAGAACATGA CTTGTATTAA AGTTTCTATT GATCCTGAAT CTAACATTAT AAGCATTTGG 
AATAATGGGA AAGGCATTCC AGTAGTAGAA CACAAGGTAG AGAAAGTTTA TGTTCCTGCT 
TTAATTTTTG GACAGCTTTT AACATCCAGT AACTATGATG ATGATGAGAA AAAAGTTACA 
GGTGGTCGTA ATGGTTATGG TGCAAAACTT TGTAATATTT TCAGTACAAA GTTTACAGTA 
GAAACAGCTT GCAAAGAATA CAAACACAGT TTTAAGCAGA CATGGATGAA TAATATGATG 
AAGACTTCTG AAGCCAAAAT TAAACATTTT GATGGTGAAG ATTACACATG CATAACATTC 
CAACCAGATC TGTCCAAATT TAAGATGGAA AAACTTGACA AGGATATTGT GGCCCTCATG 
ACTAGAAGGG CATATGATTT GGCTGGTTCG TGTAGAGGGG TCAAGGTCAT GTTTAATGGA 
AAGAAATTGC CTGTAAATGG ATTTCGCAGT TATGTAGATC TTTATGTGAA AGACAAATTG 
GATGAAACTG GGGTGGCCCT GAAAGTTATT CATGAGCTTG CAAATGAAAG ATGGGATGTT 
TGTCTCACAT TGAGTGAAAA AGGATTCCAG CAAATCAGTT TTGTAAATAG TATTGCAACT 
ACAAAAGGTG GACGGCACGT GGATTATGTG GTAGATCAAG TTGTTGGTAA ACTGATTGAA 
GTAGTTAAGA AAAAGAACAA AGCTGGTGTA TCAGTGAAAC CATTTCAAGT AAAAAACCAT 
ATATGGGTTT TTATTAATTG CCTTATTGAA AATCCAACTT TTGATTCTCA GACTAAGGAA 
AACATGACTC TGCAGCCCAA AAGTTTTGGG TCTAAATGCC AGCTGTCAGA AAAATTTTTT 
AAAGCAGCCT CTAATTGTGG CATTGTAGAA AGTATCCTGA ACTGGGTGAA ATTTAAGGCT 
CAGACTCAGC TGAATAAGAA GTGTTCATCA GTAAAATACA GTAAAATCAA AGGTATTCCC 
AAACTGGATG ATGCTAATGA TGCTGGTGGT AAACATTCCC TGGAGTGTAC ACTGATATTA 
ACAGAGGGAG ACTCTGCCAA ATCACTGGCT GTGTCTGGAT TAGGTGTGAT TGGACGAGAC 
AGATATGGAG TTTTTCCACT CAGGGGCAAA ATTCTTAATG TACGGGAAGC TTCTCATAAA 
CAGATCATGG AAAATGCTGA AATAAATAAT ATTATTAAAA TAGTTGGTCT ACAATATAAG 
AAAAGTTACG ATGATGCAGA ATCTCTGAAA ACCTTACGCT ATGGAAAGAT TATGATTATG 
ACCGATCAGG ATCAAGATGG TTCTCACATA AAAGGCCTGC TTATTAATTT CATCCATCAC 
AATTGGCCAT CACTTTTGAA GCATGGTTTT CTCGAAGAGT TCATTACTCC TATTGTAAAG 
GCAAGCAAAA ATAAGCAGGA ACTTTCCTTC TACAGTATTC CTGAATTTGA CGAATGGAAA 
AAACATATAG AAAACCAGAA AGCCTGGAAA ATAAAGTACT ATAAAGGATT GGGTACTAGT 
ACAGCTAAAG AAGCAAAGGA ATATTTTGCT GATATGGAAA GGCATCGCAT CTTGTTTAGA 
TATGCTGGTC CTGAAGATGA TGCTGCCATT ACCTTGGCAT TTAGTAAGAA GAAGATTGAT 
GACAGAAAAG AATGGTTAAC AAATTTTATG GAAGACCGGA GACAGCGTAG GCTACATGGC 
TTACCAGAGC AATTTTTATA TGGTACTGCA ACAAAGCATT TGACTTATAA TGATTTCATC 
AACAAGGAAT TGATTCTCTT CTCAAACTCA GACAATGAAA GATCTATACC ATCTCTTGTT 
GATGGCTTTA AACCTGGCCA GCGGAAAGTT TTATTTACCT GTTTCAAGAG GAATGATAAA 
CGTGAAGTAA AAGTTGCCCA GTTGGCTGGC TCTGTTGCTG AGATGTCGGC TTATCATCAT 
GGAGAACAAG CATTGATGAT GACTATTGTG AATTTGGCTC AGAACTTTGT GGGAAGTAAC 
AACATTAACT TGCTTCAGCC TATTGGTCAG TTTGGAACTC GGCTTCATGG TGGCAAAGAT 
GCTGCAAGCC CTCGTTATAT TTTCACAATG TTAAGCACTT TAGCAAGGCT ACTTTTTCCT 
GCTGTGGATG ACAACCTCCT TAAGTTCCTT TATGATGATA ATCAACGTGT AGAGCCTGAG 
TGGTATATTC CTATAATTCC CATGGTTTTA ATAAATGGTG CTGAGGGCAT TGGTACTGGA 
TGGGCTTGTA AACTACCCAA CTATGATGCT AGGGAAATTG TGAACAATGT CAGACGAATG 
CTAGATGGCC TGGATCCTCA TCCCATGCTT CCAAACTACA AAAACTTTAA AGGCACGATT 
CAAGAACTTG GTCAAAACCA GTATGCAGTC AGTGGTGAAA TATTTGTAGT GGACAGAAAC 
ACAGTAGAAA TTACAGAGCT TCCAGTTAGA ACTTGGACAC AGGTATATAA AGAACAGGTT 
TTAGAACCTA TGCTAAATGG AACAGATAAA ACACCAGCAT TAATTTCTGA TTATAAAGAA 
TATCATACTG ACACAACTGT GAAATTTGTG GTGAAAATGA CTGAAGAGAA ACTAGCACAA 
GCAGAAGCTG CTGGACTGCA TAAAGTTTTT AAACTTCAAA CTACTCTTAC TTGTAATTCC 
ATGGTACTTT TTGATCATAT GGGATGTCTG AAGAAATATG AAACTGTGCA AGACATTCTG 
AAAGAATTCT TTGATTTACG ATTAAGTTAT TACGGTTTAC GTAAGGAGTG GCTTGTGGGA 
ATGTTGGGAG CAGAATCTAC AAAGCTTAAC AATCAAGCCC GTTTCATTTT AGAGAAGATA 
CAAGGGAAAA TTACTATAGA GAATAGGTCA AAGAAAGATT TGATTCAAAT GTTAGTCCAG 
AGAGGTTATG AATCTGACCC AGTGAAAGCC TGGAAAGAAG CACAAGAAAA GGCAGCAGAA 
GAGGATGAAA CACAAAACCA GCATGATGAT AGTTCCTCCG ACTCAGGAAC TCCTTCAGGC 
CCAGATTTTA ATTATATTTT AAATATGTCT CTGTGGTCTC TTACTAAAGA AAAAGTTGAA 
GAACTGATTA AACAGAGAGA TGCAAAAGGG CGAGAGGTCA ATGATCTTAA AAGAAAATCT 
CCTTCAGATC TTTGGAAAGA GGATTTAGCG GCATTTGTTG AAGAACTGGA TAAAGTGGAA 
TCTCAAGAAC GAGAAGATGT TCTGGCTGGA ATGTCTGGAA AAGCAATTAA AGGTAAAGTT 
GGCAAACCTA AGGTGAAGAA ACTCCAGTTG GAAGAGACAA TGCCCTCACC TTATGGCAGA 
AGAATAATTC CTGAAATTAC AGCTATGAAG GCAGATGCCA GCAAAAAGTT GCTGAAGAAG 
AAGAAGGGTG ATCTTGATAC TGCAGCAGTA AAAGTGGAAT TTGATGAAGA ATTCAGTGGA 
GCGCCAGTAG AAGGTGCAGG AGAAGAGGCA TTGACTCCAT CAGTTCCTAT AAATAAAGGC 
CCCAAACCCA AAAGGGAGAA GAAGGAGCCT GGTACCAGAG TGAGAAAAAC ACCTACATCA 
TCTGGTAAAC CTAGTGCAAA GAAAGTGAAG AAACGGAATC CTTGGTCAGA TGATGAATCC 
AAGTCAGAAA GTGATTTGGA AGAAACAGAA CCTGTGGTTA TTCCAAGAGA TTCTTTGCTT 
AGGAGAGCAG CAGCCGAAAG ACCTAAATAC ACATTTGATT TCTCAGAAGA AGAGGATGAT 
GATGCTGATG ATGATGATGA TGACAATAAT GATTTAGAGG AATTGAAAGT TAAAGCATCT 
CCCATAACAA ATGATGGGGA AGATGAATTT GTTCCTTCAG ATGGGTTAGA TAAAGATGAA 
TATACATTTT CACCAGGCAA ATCAAAAGCC ACTCCAGAAA AATCTTTGCA TGACAAAAAA 
AGTCAGGATT TTGGAAATCT CTTCTCATTT CCTTCATATT CTCAGAAGTC AGAAGATGAT 
TCAGCTAAAT TTGACAGTAA TGAAGAAGAT TCTGCTTCTG TTTTTTCACC ATCATTTGGT 
CTGAAACAGA CAGATAAAGT TCCAAGTAAA ACGGTAGCTG CTAAAAAGGG AAAACCGTCT 
TCAGATACAG TCCCTAAGCC CAAGAGAGCC CCAAAACAGA AGAAAGTAGT AGAGGCTGTA 
AACTCTGACT CGGATTCAGA ATTTGGCATT CCAAAGAAGA CTACAACACC AAAAGGTAAA 
GGCCGAGGGG CAAAGAAAAG GAAAGCATCT GGCTCTGAAA ATGAAGGCGA TTATAACCCT 
GGCAGGAAAA CATCCAAAAC AACAAGCAAG AAACCGAAGA AGACATCTTT TGATCAGGAT 
TCAGATGTGG ACATCTTCCC CTCAGACTTC CCTACTGAGC CACCTTCTCT GCCACGAACC 
GGTCGGGCTA GGAAAGAAGT AAAATATTTT GCAGAGTCTG ATGAAGAAGA TGATGATGTT 
GATTTTGCAA TGTTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009443307.2
CDS	277..5157
Translation	MAKSGGCGAGAGVGGGNGALTWVTLFDQNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEDDDVDFAMFN

Target ORF information:

RefSeq Version	XM_009445032.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009445032.3

ORF Insert Sequence:

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA CTCTTTTTGA TCAGAACAAT GCTGCAAAAA AAGAAGAGTC AGAAACTGCC 
AACAAAAATG ATTCTTCAAA GAAGTTGTCT GTTGAGAGAG TGTATCAGAA GAAGACACAA 
CTTGAACACA TTCTTCTTCG TCCTGATACA TATATTGGGT CAGTGGAGCC ATTGACGCAG 
TTCATGTGGG TGTATGATGA AGATGTAGGA ATGAATTGCA GAGAGGTTAC CTTTGTGCCA 
GGTTTATACA AGATCTTTGA TGAAATTTTG GTTAATGCTG CTGACAATAA ACAGAGGGAT 
AAGAACATGA CTTGTATTAA AGTTTCTATT GATCCTGAAT CTAACATTAT AAGCATTTGG 
AATAATGGGA AAGGCATTCC AGTAGTAGAA CACAAGGTAG AGAAAGTTTA TGTTCCTGCT 
TTAATTTTTG GACAGCTTTT AACATCCAGT AACTATGATG ATGATGAGAA AAAAGTTACA 
GGTGGTCGTA ATGGTTATGG TGCAAAACTT TGTAATATTT TCAGTACAAA GTTTACAGTA 
GAAACAGCTT GCAAAGAATA CAAACACAGT TTTAAGCAGA CATGGATGAA TAATATGATG 
AAGACTTCTG AAGCCAAAAT TAAACATTTT GATGGTGAAG ATTACACATG CATAACATTC 
CAACCAGATC TGTCCAAATT TAAGATGGAA AAACTTGACA AGGATATTGT GGCCCTCATG 
ACTAGAAGGG CATATGATTT GGCTGGTTCG TGTAGAGGGG TCAAGGTCAT GTTTAATGGA 
AAGAAATTGC CTGTAAATGG ATTTCGCAGT TATGTAGATC TTTATGTGAA AGACAAATTG 
GATGAAACTG GGGTGGCCCT GAAAGTTATT CATGAGCTTG CAAATGAAAG ATGGGATGTT 
TGTCTCACAT TGAGTGAAAA AGGATTCCAG CAAATCAGTT TTGTAAATAG TATTGCAACT 
ACAAAAGGTG GACGGCACGT GGATTATGTG GTAGATCAAG TTGTTGGTAA ACTGATTGAA 
GTAGTTAAGA AAAAGAACAA AGCTGGTGTA TCAGTGAAAC CATTTCAAGT AAAAAACCAT 
ATATGGGTTT TTATTAATTG CCTTATTGAA AATCCAACTT TTGATTCTCA GACTAAGGAA 
AACATGACTC TGCAGCCCAA AAGTTTTGGG TCTAAATGCC AGCTGTCAGA AAAATTTTTT 
AAAGCAGCCT CTAATTGTGG CATTGTAGAA AGTATCCTGA ACTGGGTGAA ATTTAAGGCT 
CAGACTCAGC TGAATAAGAA GTGTTCATCA GTAAAATACA GTAAAATCAA AGGTATTCCC 
AAACTGGATG ATGCTAATGA TGCTGGTGGT AAACATTCCC TGGAGTGTAC ACTGATATTA 
ACAGAGGGAG ACTCTGCCAA ATCACTGGCT GTGTCTGGAT TAGGTGTGAT TGGACGAGAC 
AGATATGGAG TTTTTCCACT CAGGGGCAAA ATTCTTAATG TACGGGAAGC TTCTCATAAA 
CAGATCATGG AAAATGCTGA AATAAATAAT ATTATTAAAA TAGTTGGTCT ACAATATAAG 
AAAAGTTACG ATGATGCAGA ATCTCTGAAA ACCTTACGCT ATGGAAAGAT TATGATTATG 
ACCGATCAGG ATCAAGATGG TTCTCACATA AAAGGCCTGC TTATTAATTT CATCCATCAC 
AATTGGCCAT CACTTTTGAA GCATGGTTTT CTCGAAGAGT TCATTACTCC TATTGTAAAG 
GCAAGCAAAA ATAAGCAGGA ACTTTCCTTC TACAGTATTC CTGAATTTGA CGAATGGAAA 
AAACATATAG AAAACCAGAA AGCCTGGAAA ATAAAGTACT ATAAAGGATT GGGTACTAGT 
ACAGCTAAAG AAGCAAAGGA ATATTTTGCT GATATGGAAA GGCATCGCAT CTTGTTTAGA 
TATGCTGGTC CTGAAGATGA TGCTGCCATT ACCTTGGCAT TTAGTAAGAA GAAGATTGAT 
GACAGAAAAG AATGGTTAAC AAATTTTATG GAAGACCGGA GACAGCGTAG GCTACATGGC 
TTACCAGAGC AATTTTTATA TGGTACTGCA ACAAAGCATT TGACTTATAA TGATTTCATC 
AACAAGGAAT TGATTCTCTT CTCAAACTCA GACAATGAAA GATCTATACC ATCTCTTGTT 
GATGGCTTTA AACCTGGCCA GCGGAAAGTT TTATTTACCT GTTTCAAGAG GAATGATAAA 
CGTGAAGTAA AAGTTGCCCA GTTGGCTGGC TCTGTTGCTG AGATGTCGGC TTATCATCAT 
GGAGAACAAG CATTGATGAT GACTATTGTG AATTTGGCTC AGAACTTTGT GGGAAGTAAC 
AACATTAACT TGCTTCAGCC TATTGGTCAG TTTGGAACTC GGCTTCATGG TGGCAAAGAT 
GCTGCAAGCC CTCGTTATAT TTTCACAATG TTAAGCACTT TAGCAAGGCT ACTTTTTCCT 
GCTGTGGATG ACAACCTCCT TAAGTTCCTT TATGATGATA ATCAACGTGT AGAGCCTGAG 
TGGTATATTC CTATAATTCC CATGGTTTTA ATAAATGGTG CTGAGGGCAT TGGTACTGGA 
TGGGCTTGTA AACTACCCAA CTATGATGCT AGGGAAATTG TGAACAATGT CAGACGAATG 
CTAGATGGCC TGGATCCTCA TCCCATGCTT CCAAACTACA AAAACTTTAA AGGCACGATT 
CAAGAACTTG GTCAAAACCA GTATGCAGTC AGTGGTGAAA TATTTGTAGT GGACAGAAAC 
ACAGTAGAAA TTACAGAGCT TCCAGTTAGA ACTTGGACAC AGGTATATAA AGAACAGGTT 
TTAGAACCTA TGCTAAATGG AACAGATAAA ACACCAGCAT TAATTTCTGA TTATAAAGAA 
TATCATACTG ACACAACTGT GAAATTTGTG GTGAAAATGA CTGAAGAGAA ACTAGCACAA 
GCAGAAGCTG CTGGACTGCA TAAAGTTTTT AAACTTCAAA CTACTCTTAC TTGTAATTCC 
ATGGTACTTT TTGATCATAT GGGATGTCTG AAGAAATATG AAACTGTGCA AGACATTCTG 
AAAGAATTCT TTGATTTACG ATTAAGTTAT TACGGTTTAC GTAAGGAGTG GCTTGTGGGA 
ATGTTGGGAG CAGAATCTAC AAAGCTTAAC AATCAAGCCC GTTTCATTTT AGAGAAGATA 
CAAGGGAAAA TTACTATAGA GAATAGGTCA AAGAAAGATT TGATTCAAAT GTTAGTCCAG 
AGAGGTTATG AATCTGACCC AGTGAAAGCC TGGAAAGAAG CACAAGAAAA GGCAGCAGAA 
GAGGATGAAA CACAAAACCA GCATGATGAT AGTTCCTCCG ACTCAGGAAC TCCTTCAGGC 
CCAGATTTTA ATTATATTTT AAATATGTCT CTGTGGTCTC TTACTAAAGA AAAAGTTGAA 
GAACTGATTA AACAGAGAGA TGCAAAAGGG CGAGAGGTCA ATGATCTTAA AAGAAAATCT 
CCTTCAGATC TTTGGAAAGA GGATTTAGCG GCATTTGTTG AAGAACTGGA TAAAGTGGAA 
TCTCAAGAAC GAGAAGATGT TCTGGCTGGA ATGTCTGGAA AAGCAATTAA AGGTAAAGTT 
GGCAAACCTA AGGTGAAGAA ACTCCAGTTG GAAGAGACAA TGCCCTCACC TTATGGCAGA 
AGAATAATTC CTGAAATTAC AGCTATGAAG GCAGATGCCA GCAAAAAGTT GCTGAAGAAG 
AAGAAGGGTG ATCTTGATAC TGCAGCAGTA AAAGTGGAAT TTGATGAAGA ATTCAGTGGA 
GCGCCAGTAG AAGGTGCAGG AGAAGAGGCA TTGACTCCAT CAGTTCCTAT AAATAAAGGC 
CCCAAACCCA AAAGGGAGAA GAAGGAGCCT GGTACCAGAG TGAGAAAAAC ACCTACATCA 
TCTGGTAAAC CTAGTGCAAA GAAAGTGAAG AAACGGAATC CTTGGTCAGA TGATGAATCC 
AAGTCAGAAA GTGATTTGGA AGAAACAGAA CCTGTGGTTA TTCCAAGAGA TTCTTTGCTT 
AGGAGAGCAG CAGCCGAAAG ACCTAAATAC ACATTTGATT TCTCAGAAGA AGAGGATGAT 
GATGCTGATG ATGATGATGA TGACAATAAT GATTTAGAGG AATTGAAAGT TAAAGCATCT 
CCCATAACAA ATGATGGGGA AGATGAATTT GTTCCTTCAG ATGGGTTAGA TAAAGATGAA 
TATACATTTT CACCAGGCAA ATCAAAAGCC ACTCCAGAAA AATCTTTGCA TGACAAAAAA 
AGTCAGGATT TTGGAAATCT CTTCTCATTT CCTTCATATT CTCAGAAGTC AGAAGATGAT 
TCAGCTAAAT TTGACAGTAA TGAAGAAGAT TCTGCTTCTG TTTTTTCACC ATCATTTGGT 
CTGAAACAGA CAGATAAAGT TCCAAGTAAA ACGGTAGCTG CTAAAAAGGG AAAACCGTCT 
TCAGATACAG TCCCTAAGCC CAAGAGAGCC CCAAAACAGA AGAAAGTAGT AGAGGCTGTA 
AACTCTGACT CGGATTCAGA ATTTGGCATT CCAAAGAAGA CTACAACACC AAAAGGTAAA 
GGCCGAGGGG CAAAGAAAAG GAAAGCATCT GGCTCTGAAA ATGAAGGCGA TTATAACCCT 
GGCAGGAAAA CATCCAAAAC AACAAGCAAG AAACCGAAGA AGACATCTTT TGATCAGGAT 
TCAGATGTGG ACATCTTCCC CTCAGACTTC CCTACTGAGC CACCTTCTCT GCCACGAACC 
GGTCGGGCTA GGAAAGAAGT AAAATATTTT GCAGAGTCTG ATGAAGAAGA TGATGATGTT 
GATTTTGCAA TGTTTAATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_016940585.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796074.2 Pan troglodytes DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPc81501
Clone ID Related Accession (Same CDS sequence)	XM_016940585.2 , XM_516332.5
Accession Version	XM_016940585.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	DNA topoisomerase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016940585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA ACAATGCTGC AAAAAAAGAA GAGTCAGAAA CTGCCAACAA AAATGATTCT 
TCAAAGAAGT TGTCTGTTGA GAGAGTGTAT CAGAAGAAGA CACAACTTGA ACACATTCTT 
CTTCGTCCTG ATACATATAT TGGGTCAGTG GAGCCATTGA CGCAGTTCAT GTGGGTGTAT 
GATGAAGATG TAGGAATGAA TTGCAGAGAG GTTACCTTTG TGCCAGGTTT ATACAAGATC 
TTTGATGAAA TTTTGGTTAA TGCTGCTGAC AATAAACAGA GGGATAAGAA CATGACTTGT 
ATTAAAGTTT CTATTGATCC TGAATCTAAC ATTATAAGCA TTTGGAATAA TGGGAAAGGC 
ATTCCAGTAG TAGAACACAA GGTAGAGAAA GTTTATGTTC CTGCTTTAAT TTTTGGACAG 
CTTTTAACAT CCAGTAACTA TGATGATGAT GAGAAAAAAG TTACAGGTGG TCGTAATGGT 
TATGGTGCAA AACTTTGTAA TATTTTCAGT ACAAAGTTTA CAGTAGAAAC AGCTTGCAAA 
GAATACAAAC ACAGTTTTAA GCAGACATGG ATGAATAATA TGATGAAGAC TTCTGAAGCC 
AAAATTAAAC ATTTTGATGG TGAAGATTAC ACATGCATAA CATTCCAACC AGATCTGTCC 
AAATTTAAGA TGGAAAAACT TGACAAGGAT ATTGTGGCCC TCATGACTAG AAGGGCATAT 
GATTTGGCTG GTTCGTGTAG AGGGGTCAAG GTCATGTTTA ATGGAAAGAA ATTGCCTGTA 
AATGGATTTC GCAGTTATGT AGATCTTTAT GTGAAAGACA AATTGGATGA AACTGGGGTG 
GCCCTGAAAG TTATTCATGA GCTTGCAAAT GAAAGATGGG ATGTTTGTCT CACATTGAGT 
GAAAAAGGAT TCCAGCAAAT CAGTTTTGTA AATAGTATTG CAACTACAAA AGGTGGACGG 
CACGTGGATT ATGTGGTAGA TCAAGTTGTT GGTAAACTGA TTGAAGTAGT TAAGAAAAAG 
AACAAAGCTG GTGTATCAGT GAAACCATTT CAAGTAAAAA ACCATATATG GGTTTTTATT 
AATTGCCTTA TTGAAAATCC AACTTTTGAT TCTCAGACTA AGGAAAACAT GACTCTGCAG 
CCCAAAAGTT TTGGGTCTAA ATGCCAGCTG TCAGAAAAAT TTTTTAAAGC AGCCTCTAAT 
TGTGGCATTG TAGAAAGTAT CCTGAACTGG GTGAAATTTA AGGCTCAGAC TCAGCTGAAT 
AAGAAGTGTT CATCAGTAAA ATACAGTAAA ATCAAAGGTA TTCCCAAACT GGATGATGCT 
AATGATGCTG GTGGTAAACA TTCCCTGGAG TGTACACTGA TATTAACAGA GGGAGACTCT 
GCCAAATCAC TGGCTGTGTC TGGATTAGGT GTGATTGGAC GAGACAGATA TGGAGTTTTT 
CCACTCAGGG GCAAAATTCT TAATGTACGG GAAGCTTCTC ATAAACAGAT CATGGAAAAT 
GCTGAAATAA ATAATATTAT TAAAATAGTT GGTCTACAAT ATAAGAAAAG TTACGATGAT 
GCAGAATCTC TGAAAACCTT ACGCTATGGA AAGATTATGA TTATGACCGA TCAGGATCAA 
GATGGTTCTC ACATAAAAGG CCTGCTTATT AATTTCATCC ATCACAATTG GCCATCACTT 
TTGAAGCATG GTTTTCTCGA AGAGTTCATT ACTCCTATTG TAAAGGCAAG CAAAAATAAG 
CAGGAACTTT CCTTCTACAG TATTCCTGAA TTTGACGAAT GGAAAAAACA TATAGAAAAC 
CAGAAAGCCT GGAAAATAAA GTACTATAAA GGATTGGGTA CTAGTACAGC TAAAGAAGCA 
AAGGAATATT TTGCTGATAT GGAAAGGCAT CGCATCTTGT TTAGATATGC TGGTCCTGAA 
GATGATGCTG CCATTACCTT GGCATTTAGT AAGAAGAAGA TTGATGACAG AAAAGAATGG 
TTAACAAATT TTATGGAAGA CCGGAGACAG CGTAGGCTAC ATGGCTTACC AGAGCAATTT 
TTATATGGTA CTGCAACAAA GCATTTGACT TATAATGATT TCATCAACAA GGAATTGATT 
CTCTTCTCAA ACTCAGACAA TGAAAGATCT ATACCATCTC TTGTTGATGG CTTTAAACCT 
GGCCAGCGGA AAGTTTTATT TACCTGTTTC AAGAGGAATG ATAAACGTGA AGTAAAAGTT 
GCCCAGTTGG CTGGCTCTGT TGCTGAGATG TCGGCTTATC ATCATGGAGA ACAAGCATTG 
ATGATGACTA TTGTGAATTT GGCTCAGAAC TTTGTGGGAA GTAACAACAT TAACTTGCTT 
CAGCCTATTG GTCAGTTTGG AACTCGGCTT CATGGTGGCA AAGATGCTGC AAGCCCTCGT 
TATATTTTCA CAATGTTAAG CACTTTAGCA AGGCTACTTT TTCCTGCTGT GGATGACAAC 
CTCCTTAAGT TCCTTTATGA TGATAATCAA CGTGTAGAGC CTGAGTGGTA TATTCCTATA 
ATTCCCATGG TTTTAATAAA TGGTGCTGAG GGCATTGGTA CTGGATGGGC TTGTAAACTA 
CCCAACTATG ATGCTAGGGA AATTGTGAAC AATGTCAGAC GAATGCTAGA TGGCCTGGAT 
CCTCATCCCA TGCTTCCAAA CTACAAAAAC TTTAAAGGCA CGATTCAAGA ACTTGGTCAA 
AACCAGTATG CAGTCAGTGG TGAAATATTT GTAGTGGACA GAAACACAGT AGAAATTACA 
GAGCTTCCAG TTAGAACTTG GACACAGGTA TATAAAGAAC AGGTTTTAGA ACCTATGCTA 
AATGGAACAG ATAAAACACC AGCATTAATT TCTGATTATA AAGAATATCA TACTGACACA 
ACTGTGAAAT TTGTGGTGAA AATGACTGAA GAGAAACTAG CACAAGCAGA AGCTGCTGGA 
CTGCATAAAG TTTTTAAACT TCAAACTACT CTTACTTGTA ATTCCATGGT ACTTTTTGAT 
CATATGGGAT GTCTGAAGAA ATATGAAACT GTGCAAGACA TTCTGAAAGA ATTCTTTGAT 
TTACGATTAA GTTATTACGG TTTACGTAAG GAGTGGCTTG TGGGAATGTT GGGAGCAGAA 
TCTACAAAGC TTAACAATCA AGCCCGTTTC ATTTTAGAGA AGATACAAGG GAAAATTACT 
ATAGAGAATA GGTCAAAGAA AGATTTGATT CAAATGTTAG TCCAGAGAGG TTATGAATCT 
GACCCAGTGA AAGCCTGGAA AGAAGCACAA GAAAAGGCAG CAGAAGAGGA TGAAACACAA 
AACCAGCATG ATGATAGTTC CTCCGACTCA GGAACTCCTT CAGGCCCAGA TTTTAATTAT 
ATTTTAAATA TGTCTCTGTG GTCTCTTACT AAAGAAAAAG TTGAAGAACT GATTAAACAG 
AGAGATGCAA AAGGGCGAGA GGTCAATGAT CTTAAAAGAA AATCTCCTTC AGATCTTTGG 
AAAGAGGATT TAGCGGCATT TGTTGAAGAA CTGGATAAAG TGGAATCTCA AGAACGAGAA 
GATGTTCTGG CTGGAATGTC TGGAAAAGCA ATTAAAGGTA AAGTTGGCAA ACCTAAGGTG 
AAGAAACTCC AGTTGGAAGA GACAATGCCC TCACCTTATG GCAGAAGAAT AATTCCTGAA 
ATTACAGCTA TGAAGGCAGA TGCCAGCAAA AAGTTGCTGA AGAAGAAGAA GGGTGATCTT 
GATACTGCAG CAGTAAAAGT GGAATTTGAT GAAGAATTCA GTGGAGCGCC AGTAGAAGGT 
GCAGGAGAAG AGGCATTGAC TCCATCAGTT CCTATAAATA AAGGCCCCAA ACCCAAAAGG 
GAGAAGAAGG AGCCTGGTAC CAGAGTGAGA AAAACACCTA CATCATCTGG TAAACCTAGT 
GCAAAGAAAG TGAAGAAACG GAATCCTTGG TCAGATGATG AATCCAAGTC AGAAAGTGAT 
TTGGAAGAAA CAGAACCTGT GGTTATTCCA AGAGATTCTT TGCTTAGGAG AGCAGCAGCC 
GAAAGACCTA AATACACATT TGATTTCTCA GAAGAAGAGG ATGATGATGC TGATGATGAT 
GATGATGACA ATAATGATTT AGAGGAATTG AAAGTTAAAG CATCTCCCAT AACAAATGAT 
GGGGAAGATG AATTTGTTCC TTCAGATGGG TTAGATAAAG ATGAATATAC ATTTTCACCA 
GGCAAATCAA AAGCCACTCC AGAAAAATCT TTGCATGACA AAAAAAGTCA GGATTTTGGA 
AATCTCTTCT CATTTCCTTC ATATTCTCAG AAGTCAGAAG ATGATTCAGC TAAATTTGAC 
AGTAATGAAG AAGATTCTGC TTCTGTTTTT TCACCATCAT TTGGTCTGAA ACAGACAGAT 
AAAGTTCCAA GTAAAACGGT AGCTGCTAAA AAGGGAAAAC CGTCTTCAGA TACAGTCCCT 
AAGCCCAAGA GAGCCCCAAA ACAGAAGAAA GTAGTAGAGG CTGTAAACTC TGACTCGGAT 
TCAGAATTTG GCATTCCAAA GAAGACTACA ACACCAAAAG GTAAAGGCCG AGGGGCAAAG 
AAAAGGAAAG CATCTGGCTC TGAAAATGAA GGCGATTATA ACCCTGGCAG GAAAACATCC 
AAAACAACAA GCAAGAAACC GAAGAAGACA TCTTTTGATC AGGATTCAGA TGTGGACATC 
TTCCCCTCAG ACTTCCCTAC TGAGCCACCT TCTCTGCCAC GAACCGGTCG GGCTAGGAAA 
GAAGTAAAAT ATTTTGCAGA GTCTGATGAA GAAGATGATG ATGTTGATTT TGCAATGTTT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796074.2
CDS	277..5142
Translation	MAKSGGCGAGAGVGGGNGALTWVNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEDDDVDFAMFN

Target ORF information:

RefSeq Version	XM_016940585.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016940585.2

ORF Insert Sequence:

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA ACAATGCTGC AAAAAAAGAA GAGTCAGAAA CTGCCAACAA AAATGATTCT 
TCAAAGAAGT TGTCTGTTGA GAGAGTGTAT CAGAAGAAGA CACAACTTGA ACACATTCTT 
CTTCGTCCTG ATACATATAT TGGGTCAGTG GAGCCATTGA CGCAGTTCAT GTGGGTGTAT 
GATGAAGATG TAGGAATGAA TTGCAGAGAG GTTACCTTTG TGCCAGGTTT ATACAAGATC 
TTTGATGAAA TTTTGGTTAA TGCTGCTGAC AATAAACAGA GGGATAAGAA CATGACTTGT 
ATTAAAGTTT CTATTGATCC TGAATCTAAC ATTATAAGCA TTTGGAATAA TGGGAAAGGC 
ATTCCAGTAG TAGAACACAA GGTAGAGAAA GTTTATGTTC CTGCTTTAAT TTTTGGACAG 
CTTTTAACAT CCAGTAACTA TGATGATGAT GAGAAAAAAG TTACAGGTGG TCGTAATGGT 
TATGGTGCAA AACTTTGTAA TATTTTCAGT ACAAAGTTTA CAGTAGAAAC AGCTTGCAAA 
GAATACAAAC ACAGTTTTAA GCAGACATGG ATGAATAATA TGATGAAGAC TTCTGAAGCC 
AAAATTAAAC ATTTTGATGG TGAAGATTAC ACATGCATAA CATTCCAACC AGATCTGTCC 
AAATTTAAGA TGGAAAAACT TGACAAGGAT ATTGTGGCCC TCATGACTAG AAGGGCATAT 
GATTTGGCTG GTTCGTGTAG AGGGGTCAAG GTCATGTTTA ATGGAAAGAA ATTGCCTGTA 
AATGGATTTC GCAGTTATGT AGATCTTTAT GTGAAAGACA AATTGGATGA AACTGGGGTG 
GCCCTGAAAG TTATTCATGA GCTTGCAAAT GAAAGATGGG ATGTTTGTCT CACATTGAGT 
GAAAAAGGAT TCCAGCAAAT CAGTTTTGTA AATAGTATTG CAACTACAAA AGGTGGACGG 
CACGTGGATT ATGTGGTAGA TCAAGTTGTT GGTAAACTGA TTGAAGTAGT TAAGAAAAAG 
AACAAAGCTG GTGTATCAGT GAAACCATTT CAAGTAAAAA ACCATATATG GGTTTTTATT 
AATTGCCTTA TTGAAAATCC AACTTTTGAT TCTCAGACTA AGGAAAACAT GACTCTGCAG 
CCCAAAAGTT TTGGGTCTAA ATGCCAGCTG TCAGAAAAAT TTTTTAAAGC AGCCTCTAAT 
TGTGGCATTG TAGAAAGTAT CCTGAACTGG GTGAAATTTA AGGCTCAGAC TCAGCTGAAT 
AAGAAGTGTT CATCAGTAAA ATACAGTAAA ATCAAAGGTA TTCCCAAACT GGATGATGCT 
AATGATGCTG GTGGTAAACA TTCCCTGGAG TGTACACTGA TATTAACAGA GGGAGACTCT 
GCCAAATCAC TGGCTGTGTC TGGATTAGGT GTGATTGGAC GAGACAGATA TGGAGTTTTT 
CCACTCAGGG GCAAAATTCT TAATGTACGG GAAGCTTCTC ATAAACAGAT CATGGAAAAT 
GCTGAAATAA ATAATATTAT TAAAATAGTT GGTCTACAAT ATAAGAAAAG TTACGATGAT 
GCAGAATCTC TGAAAACCTT ACGCTATGGA AAGATTATGA TTATGACCGA TCAGGATCAA 
GATGGTTCTC ACATAAAAGG CCTGCTTATT AATTTCATCC ATCACAATTG GCCATCACTT 
TTGAAGCATG GTTTTCTCGA AGAGTTCATT ACTCCTATTG TAAAGGCAAG CAAAAATAAG 
CAGGAACTTT CCTTCTACAG TATTCCTGAA TTTGACGAAT GGAAAAAACA TATAGAAAAC 
CAGAAAGCCT GGAAAATAAA GTACTATAAA GGATTGGGTA CTAGTACAGC TAAAGAAGCA 
AAGGAATATT TTGCTGATAT GGAAAGGCAT CGCATCTTGT TTAGATATGC TGGTCCTGAA 
GATGATGCTG CCATTACCTT GGCATTTAGT AAGAAGAAGA TTGATGACAG AAAAGAATGG 
TTAACAAATT TTATGGAAGA CCGGAGACAG CGTAGGCTAC ATGGCTTACC AGAGCAATTT 
TTATATGGTA CTGCAACAAA GCATTTGACT TATAATGATT TCATCAACAA GGAATTGATT 
CTCTTCTCAA ACTCAGACAA TGAAAGATCT ATACCATCTC TTGTTGATGG CTTTAAACCT 
GGCCAGCGGA AAGTTTTATT TACCTGTTTC AAGAGGAATG ATAAACGTGA AGTAAAAGTT 
GCCCAGTTGG CTGGCTCTGT TGCTGAGATG TCGGCTTATC ATCATGGAGA ACAAGCATTG 
ATGATGACTA TTGTGAATTT GGCTCAGAAC TTTGTGGGAA GTAACAACAT TAACTTGCTT 
CAGCCTATTG GTCAGTTTGG AACTCGGCTT CATGGTGGCA AAGATGCTGC AAGCCCTCGT 
TATATTTTCA CAATGTTAAG CACTTTAGCA AGGCTACTTT TTCCTGCTGT GGATGACAAC 
CTCCTTAAGT TCCTTTATGA TGATAATCAA CGTGTAGAGC CTGAGTGGTA TATTCCTATA 
ATTCCCATGG TTTTAATAAA TGGTGCTGAG GGCATTGGTA CTGGATGGGC TTGTAAACTA 
CCCAACTATG ATGCTAGGGA AATTGTGAAC AATGTCAGAC GAATGCTAGA TGGCCTGGAT 
CCTCATCCCA TGCTTCCAAA CTACAAAAAC TTTAAAGGCA CGATTCAAGA ACTTGGTCAA 
AACCAGTATG CAGTCAGTGG TGAAATATTT GTAGTGGACA GAAACACAGT AGAAATTACA 
GAGCTTCCAG TTAGAACTTG GACACAGGTA TATAAAGAAC AGGTTTTAGA ACCTATGCTA 
AATGGAACAG ATAAAACACC AGCATTAATT TCTGATTATA AAGAATATCA TACTGACACA 
ACTGTGAAAT TTGTGGTGAA AATGACTGAA GAGAAACTAG CACAAGCAGA AGCTGCTGGA 
CTGCATAAAG TTTTTAAACT TCAAACTACT CTTACTTGTA ATTCCATGGT ACTTTTTGAT 
CATATGGGAT GTCTGAAGAA ATATGAAACT GTGCAAGACA TTCTGAAAGA ATTCTTTGAT 
TTACGATTAA GTTATTACGG TTTACGTAAG GAGTGGCTTG TGGGAATGTT GGGAGCAGAA 
TCTACAAAGC TTAACAATCA AGCCCGTTTC ATTTTAGAGA AGATACAAGG GAAAATTACT 
ATAGAGAATA GGTCAAAGAA AGATTTGATT CAAATGTTAG TCCAGAGAGG TTATGAATCT 
GACCCAGTGA AAGCCTGGAA AGAAGCACAA GAAAAGGCAG CAGAAGAGGA TGAAACACAA 
AACCAGCATG ATGATAGTTC CTCCGACTCA GGAACTCCTT CAGGCCCAGA TTTTAATTAT 
ATTTTAAATA TGTCTCTGTG GTCTCTTACT AAAGAAAAAG TTGAAGAACT GATTAAACAG 
AGAGATGCAA AAGGGCGAGA GGTCAATGAT CTTAAAAGAA AATCTCCTTC AGATCTTTGG 
AAAGAGGATT TAGCGGCATT TGTTGAAGAA CTGGATAAAG TGGAATCTCA AGAACGAGAA 
GATGTTCTGG CTGGAATGTC TGGAAAAGCA ATTAAAGGTA AAGTTGGCAA ACCTAAGGTG 
AAGAAACTCC AGTTGGAAGA GACAATGCCC TCACCTTATG GCAGAAGAAT AATTCCTGAA 
ATTACAGCTA TGAAGGCAGA TGCCAGCAAA AAGTTGCTGA AGAAGAAGAA GGGTGATCTT 
GATACTGCAG CAGTAAAAGT GGAATTTGAT GAAGAATTCA GTGGAGCGCC AGTAGAAGGT 
GCAGGAGAAG AGGCATTGAC TCCATCAGTT CCTATAAATA AAGGCCCCAA ACCCAAAAGG 
GAGAAGAAGG AGCCTGGTAC CAGAGTGAGA AAAACACCTA CATCATCTGG TAAACCTAGT 
GCAAAGAAAG TGAAGAAACG GAATCCTTGG TCAGATGATG AATCCAAGTC AGAAAGTGAT 
TTGGAAGAAA CAGAACCTGT GGTTATTCCA AGAGATTCTT TGCTTAGGAG AGCAGCAGCC 
GAAAGACCTA AATACACATT TGATTTCTCA GAAGAAGAGG ATGATGATGC TGATGATGAT 
GATGATGACA ATAATGATTT AGAGGAATTG AAAGTTAAAG CATCTCCCAT AACAAATGAT 
GGGGAAGATG AATTTGTTCC TTCAGATGGG TTAGATAAAG ATGAATATAC ATTTTCACCA 
GGCAAATCAA AAGCCACTCC AGAAAAATCT TTGCATGACA AAAAAAGTCA GGATTTTGGA 
AATCTCTTCT CATTTCCTTC ATATTCTCAG AAGTCAGAAG ATGATTCAGC TAAATTTGAC 
AGTAATGAAG AAGATTCTGC TTCTGTTTTT TCACCATCAT TTGGTCTGAA ACAGACAGAT 
AAAGTTCCAA GTAAAACGGT AGCTGCTAAA AAGGGAAAAC CGTCTTCAGA TACAGTCCCT 
AAGCCCAAGA GAGCCCCAAA ACAGAAGAAA GTAGTAGAGG CTGTAAACTC TGACTCGGAT 
TCAGAATTTG GCATTCCAAA GAAGACTACA ACACCAAAAG GTAAAGGCCG AGGGGCAAAG 
AAAAGGAAAG CATCTGGCTC TGAAAATGAA GGCGATTATA ACCCTGGCAG GAAAACATCC 
AAAACAACAA GCAAGAAACC GAAGAAGACA TCTTTTGATC AGGATTCAGA TGTGGACATC 
TTCCCCTCAG ACTTCCCTAC TGAGCCACCT TCTCTGCCAC GAACCGGTCG GGCTAGGAAA 
GAAGTAAAAT ATTTTGCAGA GTCTGATGAA GAAGATGATG ATGTTGATTT TGCAATGTTT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_516332.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_516332.4 Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA.

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CloneID	OPc81501
Clone ID Related Accession (Same CDS sequence)	XM_016940585.2 , XM_516332.5
Accession Version	XM_516332.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	DNA topoisomerase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_516332.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA ACAATGCTGC AAAAAAAGAA GAGTCAGAAA CTGCCAACAA AAATGATTCT 
TCAAAGAAGT TGTCTGTTGA GAGAGTGTAT CAGAAGAAGA CACAACTTGA ACACATTCTT 
CTTCGTCCTG ATACATATAT TGGGTCAGTG GAGCCATTGA CGCAGTTCAT GTGGGTGTAT 
GATGAAGATG TAGGAATGAA TTGCAGAGAG GTTACCTTTG TGCCAGGTTT ATACAAGATC 
TTTGATGAAA TTTTGGTTAA TGCTGCTGAC AATAAACAGA GGGATAAGAA CATGACTTGT 
ATTAAAGTTT CTATTGATCC TGAATCTAAC ATTATAAGCA TTTGGAATAA TGGGAAAGGC 
ATTCCAGTAG TAGAACACAA GGTAGAGAAA GTTTATGTTC CTGCTTTAAT TTTTGGACAG 
CTTTTAACAT CCAGTAACTA TGATGATGAT GAGAAAAAAG TTACAGGTGG TCGTAATGGT 
TATGGTGCAA AACTTTGTAA TATTTTCAGT ACAAAGTTTA CAGTAGAAAC AGCTTGCAAA 
GAATACAAAC ACAGTTTTAA GCAGACATGG ATGAATAATA TGATGAAGAC TTCTGAAGCC 
AAAATTAAAC ATTTTGATGG TGAAGATTAC ACATGCATAA CATTCCAACC AGATCTGTCC 
AAATTTAAGA TGGAAAAACT TGACAAGGAT ATTGTGGCCC TCATGACTAG AAGGGCATAT 
GATTTGGCTG GTTCGTGTAG AGGGGTCAAG GTCATGTTTA ATGGAAAGAA ATTGCCTGTA 
AATGGATTTC GCAGTTATGT AGATCTTTAT GTGAAAGACA AATTGGATGA AACTGGGGTG 
GCCCTGAAAG TTATTCATGA GCTTGCAAAT GAAAGATGGG ATGTTTGTCT CACATTGAGT 
GAAAAAGGAT TCCAGCAAAT CAGTTTTGTA AATAGTATTG CAACTACAAA AGGTGGACGG 
CACGTGGATT ATGTGGTAGA TCAAGTTGTT GGTAAACTGA TTGAAGTAGT TAAGAAAAAG 
AACAAAGCTG GTGTATCAGT GAAACCATTT CAAGTAAAAA ACCATATATG GGTTTTTATT 
AATTGCCTTA TTGAAAATCC AACTTTTGAT TCTCAGACTA AGGAAAACAT GACTCTGCAG 
CCCAAAAGTT TTGGGTCTAA ATGCCAGCTG TCAGAAAAAT TTTTTAAAGC AGCCTCTAAT 
TGTGGCATTG TAGAAAGTAT CCTGAACTGG GTGAAATTTA AGGCTCAGAC TCAGCTGAAT 
AAGAAGTGTT CATCAGTAAA ATACAGTAAA ATCAAAGGTA TTCCCAAACT GGATGATGCT 
AATGATGCTG GTGGTAAACA TTCCCTGGAG TGTACACTGA TATTAACAGA GGGAGACTCT 
GCCAAATCAC TGGCTGTGTC TGGATTAGGT GTGATTGGAC GAGACAGATA TGGAGTTTTT 
CCACTCAGGG GCAAAATTCT TAATGTACGG GAAGCTTCTC ATAAACAGAT CATGGAAAAT 
GCTGAAATAA ATAATATTAT TAAAATAGTT GGTCTACAAT ATAAGAAAAG TTACGATGAT 
GCAGAATCTC TGAAAACCTT ACGCTATGGA AAGATTATGA TTATGACCGA TCAGGATCAA 
GATGGTTCTC ACATAAAAGG CCTGCTTATT AATTTCATCC ATCACAATTG GCCATCACTT 
TTGAAGCATG GTTTTCTCGA AGAGTTCATT ACTCCTATTG TAAAGGCAAG CAAAAATAAG 
CAGGAACTTT CCTTCTACAG TATTCCTGAA TTTGACGAAT GGAAAAAACA TATAGAAAAC 
CAGAAAGCCT GGAAAATAAA GTACTATAAA GGATTGGGTA CTAGTACAGC TAAAGAAGCA 
AAGGAATATT TTGCTGATAT GGAAAGGCAT CGCATCTTGT TTAGATATGC TGGTCCTGAA 
GATGATGCTG CCATTACCTT GGCATTTAGT AAGAAGAAGA TTGATGACAG AAAAGAATGG 
TTAACAAATT TTATGGAAGA CCGGAGACAG CGTAGGCTAC ATGGCTTACC AGAGCAATTT 
TTATATGGTA CTGCAACAAA GCATTTGACT TATAATGATT TCATCAACAA GGAATTGATT 
CTCTTCTCAA ACTCAGACAA TGAAAGATCT ATACCATCTC TTGTTGATGG CTTTAAACCT 
GGCCAGCGGA AAGTTTTATT TACCTGTTTC AAGAGGAATG ATAAACGTGA AGTAAAAGTT 
GCCCAGTTGG CTGGCTCTGT TGCTGAGATG TCGGCTTATC ATCATGGAGA ACAAGCATTG 
ATGATGACTA TTGTGAATTT GGCTCAGAAC TTTGTGGGAA GTAACAACAT TAACTTGCTT 
CAGCCTATTG GTCAGTTTGG AACTCGGCTT CATGGTGGCA AAGATGCTGC AAGCCCTCGT 
TATATTTTCA CAATGTTAAG CACTTTAGCA AGGCTACTTT TTCCTGCTGT GGATGACAAC 
CTCCTTAAGT TCCTTTATGA TGATAATCAA CGTGTAGAGC CTGAGTGGTA TATTCCTATA 
ATTCCCATGG TTTTAATAAA TGGTGCTGAG GGCATTGGTA CTGGATGGGC TTGTAAACTA 
CCCAACTATG ATGCTAGGGA AATTGTGAAC AATGTCAGAC GAATGCTAGA TGGCCTGGAT 
CCTCATCCCA TGCTTCCAAA CTACAAAAAC TTTAAAGGCA CGATTCAAGA ACTTGGTCAA 
AACCAGTATG CAGTCAGTGG TGAAATATTT GTAGTGGACA GAAACACAGT AGAAATTACA 
GAGCTTCCAG TTAGAACTTG GACACAGGTA TATAAAGAAC AGGTTTTAGA ACCTATGCTA 
AATGGAACAG ATAAAACACC AGCATTAATT TCTGATTATA AAGAATATCA TACTGACACA 
ACTGTGAAAT TTGTGGTGAA AATGACTGAA GAGAAACTAG CACAAGCAGA AGCTGCTGGA 
CTGCATAAAG TTTTTAAACT TCAAACTACT CTTACTTGTA ATTCCATGGT ACTTTTTGAT 
CATATGGGAT GTCTGAAGAA ATATGAAACT GTGCAAGACA TTCTGAAAGA ATTCTTTGAT 
TTACGATTAA GTTATTACGG TTTACGTAAG GAGTGGCTTG TGGGAATGTT GGGAGCAGAA 
TCTACAAAGC TTAACAATCA AGCCCGTTTC ATTTTAGAGA AGATACAAGG GAAAATTACT 
ATAGAGAATA GGTCAAAGAA AGATTTGATT CAAATGTTAG TCCAGAGAGG TTATGAATCT 
GACCCAGTGA AAGCCTGGAA AGAAGCACAA GAAAAGGCAG CAGAAGAGGA TGAAACACAA 
AACCAGCATG ATGATAGTTC CTCCGACTCA GGAACTCCTT CAGGCCCAGA TTTTAATTAT 
ATTTTAAATA TGTCTCTGTG GTCTCTTACT AAAGAAAAAG TTGAAGAACT GATTAAACAG 
AGAGATGCAA AAGGGCGAGA GGTCAATGAT CTTAAAAGAA AATCTCCTTC AGATCTTTGG 
AAAGAGGATT TAGCGGCATT TGTTGAAGAA CTGGATAAAG TGGAATCTCA AGAACGAGAA 
GATGTTCTGG CTGGAATGTC TGGAAAAGCA ATTAAAGGTA AAGTTGGCAA ACCTAAGGTG 
AAGAAACTCC AGTTGGAAGA GACAATGCCC TCACCTTATG GCAGAAGAAT AATTCCTGAA 
ATTACAGCTA TGAAGGCAGA TGCCAGCAAA AAGTTGCTGA AGAAGAAGAA GGGTGATCTT 
GATACTGCAG CAGTAAAAGT GGAATTTGAT GAAGAATTCA GTGGAGCGCC AGTAGAAGGT 
GCAGGAGAAG AGGCATTGAC TCCATCAGTT CCTATAAATA AAGGCCCCAA ACCCAAAAGG 
GAGAAGAAGG AGCCTGGTAC CAGAGTGAGA AAAACACCTA CATCATCTGG TAAACCTAGT 
GCAAAGAAAG TGAAGAAACG GAATCCTTGG TCAGATGATG AATCCAAGTC AGAAAGTGAT 
TTGGAAGAAA CAGAACCTGT GGTTATTCCA AGAGATTCTT TGCTTAGGAG AGCAGCAGCC 
GAAAGACCTA AATACACATT TGATTTCTCA GAAGAAGAGG ATGATGATGC TGATGATGAT 
GATGATGACA ATAATGATTT AGAGGAATTG AAAGTTAAAG CATCTCCCAT AACAAATGAT 
GGGGAAGATG AATTTGTTCC TTCAGATGGG TTAGATAAAG ATGAATATAC ATTTTCACCA 
GGCAAATCAA AAGCCACTCC AGAAAAATCT TTGCATGACA AAAAAAGTCA GGATTTTGGA 
AATCTCTTCT CATTTCCTTC ATATTCTCAG AAGTCAGAAG ATGATTCAGC TAAATTTGAC 
AGTAATGAAG AAGATTCTGC TTCTGTTTTT TCACCATCAT TTGGTCTGAA ACAGACAGAT 
AAAGTTCCAA GTAAAACGGT AGCTGCTAAA AAGGGAAAAC CGTCTTCAGA TACAGTCCCT 
AAGCCCAAGA GAGCCCCAAA ACAGAAGAAA GTAGTAGAGG CTGTAAACTC TGACTCGGAT 
TCAGAATTTG GCATTCCAAA GAAGACTACA ACACCAAAAG GTAAAGGCCG AGGGGCAAAG 
AAAAGGAAAG CATCTGGCTC TGAAAATGAA GGCGATTATA ACCCTGGCAG GAAAACATCC 
AAAACAACAA GCAAGAAACC GAAGAAGACA TCTTTTGATC AGGATTCAGA TGTGGACATC 
TTCCCCTCAG ACTTCCCTAC TGAGCCACCT TCTCTGCCAC GAACCGGTCG GGCTAGGAAA 
GAAGTAAAAT ATTTTGCAGA GTCTGATGAA GAAGATGATG ATGTTGATTT TGCAATGTTT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_516332.4
CDS	638..5503
Translation	MAKSGGCGAGAGVGGGNGALTWVNNAAKKEESETANKNDSSKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEDDDVDFAMFN

Target ORF information:

RefSeq Version	XM_516332.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_516332.5

ORF Insert Sequence:

ATGGCCAAGT CGGGTGGCTG CGGCGCGGGA GCCGGCGTGG GCGGCGGCAA CGGGGCACTG 
ACCTGGGTGA ACAATGCTGC AAAAAAAGAA GAGTCAGAAA CTGCCAACAA AAATGATTCT 
TCAAAGAAGT TGTCTGTTGA GAGAGTGTAT CAGAAGAAGA CACAACTTGA ACACATTCTT 
CTTCGTCCTG ATACATATAT TGGGTCAGTG GAGCCATTGA CGCAGTTCAT GTGGGTGTAT 
GATGAAGATG TAGGAATGAA TTGCAGAGAG GTTACCTTTG TGCCAGGTTT ATACAAGATC 
TTTGATGAAA TTTTGGTTAA TGCTGCTGAC AATAAACAGA GGGATAAGAA CATGACTTGT 
ATTAAAGTTT CTATTGATCC TGAATCTAAC ATTATAAGCA TTTGGAATAA TGGGAAAGGC 
ATTCCAGTAG TAGAACACAA GGTAGAGAAA GTTTATGTTC CTGCTTTAAT TTTTGGACAG 
CTTTTAACAT CCAGTAACTA TGATGATGAT GAGAAAAAAG TTACAGGTGG TCGTAATGGT 
TATGGTGCAA AACTTTGTAA TATTTTCAGT ACAAAGTTTA CAGTAGAAAC AGCTTGCAAA 
GAATACAAAC ACAGTTTTAA GCAGACATGG ATGAATAATA TGATGAAGAC TTCTGAAGCC 
AAAATTAAAC ATTTTGATGG TGAAGATTAC ACATGCATAA CATTCCAACC AGATCTGTCC 
AAATTTAAGA TGGAAAAACT TGACAAGGAT ATTGTGGCCC TCATGACTAG AAGGGCATAT 
GATTTGGCTG GTTCGTGTAG AGGGGTCAAG GTCATGTTTA ATGGAAAGAA ATTGCCTGTA 
AATGGATTTC GCAGTTATGT AGATCTTTAT GTGAAAGACA AATTGGATGA AACTGGGGTG 
GCCCTGAAAG TTATTCATGA GCTTGCAAAT GAAAGATGGG ATGTTTGTCT CACATTGAGT 
GAAAAAGGAT TCCAGCAAAT CAGTTTTGTA AATAGTATTG CAACTACAAA AGGTGGACGG 
CACGTGGATT ATGTGGTAGA TCAAGTTGTT GGTAAACTGA TTGAAGTAGT TAAGAAAAAG 
AACAAAGCTG GTGTATCAGT GAAACCATTT CAAGTAAAAA ACCATATATG GGTTTTTATT 
AATTGCCTTA TTGAAAATCC AACTTTTGAT TCTCAGACTA AGGAAAACAT GACTCTGCAG 
CCCAAAAGTT TTGGGTCTAA ATGCCAGCTG TCAGAAAAAT TTTTTAAAGC AGCCTCTAAT 
TGTGGCATTG TAGAAAGTAT CCTGAACTGG GTGAAATTTA AGGCTCAGAC TCAGCTGAAT 
AAGAAGTGTT CATCAGTAAA ATACAGTAAA ATCAAAGGTA TTCCCAAACT GGATGATGCT 
AATGATGCTG GTGGTAAACA TTCCCTGGAG TGTACACTGA TATTAACAGA GGGAGACTCT 
GCCAAATCAC TGGCTGTGTC TGGATTAGGT GTGATTGGAC GAGACAGATA TGGAGTTTTT 
CCACTCAGGG GCAAAATTCT TAATGTACGG GAAGCTTCTC ATAAACAGAT CATGGAAAAT 
GCTGAAATAA ATAATATTAT TAAAATAGTT GGTCTACAAT ATAAGAAAAG TTACGATGAT 
GCAGAATCTC TGAAAACCTT ACGCTATGGA AAGATTATGA TTATGACCGA TCAGGATCAA 
GATGGTTCTC ACATAAAAGG CCTGCTTATT AATTTCATCC ATCACAATTG GCCATCACTT 
TTGAAGCATG GTTTTCTCGA AGAGTTCATT ACTCCTATTG TAAAGGCAAG CAAAAATAAG 
CAGGAACTTT CCTTCTACAG TATTCCTGAA TTTGACGAAT GGAAAAAACA TATAGAAAAC 
CAGAAAGCCT GGAAAATAAA GTACTATAAA GGATTGGGTA CTAGTACAGC TAAAGAAGCA 
AAGGAATATT TTGCTGATAT GGAAAGGCAT CGCATCTTGT TTAGATATGC TGGTCCTGAA 
GATGATGCTG CCATTACCTT GGCATTTAGT AAGAAGAAGA TTGATGACAG AAAAGAATGG 
TTAACAAATT TTATGGAAGA CCGGAGACAG CGTAGGCTAC ATGGCTTACC AGAGCAATTT 
TTATATGGTA CTGCAACAAA GCATTTGACT TATAATGATT TCATCAACAA GGAATTGATT 
CTCTTCTCAA ACTCAGACAA TGAAAGATCT ATACCATCTC TTGTTGATGG CTTTAAACCT 
GGCCAGCGGA AAGTTTTATT TACCTGTTTC AAGAGGAATG ATAAACGTGA AGTAAAAGTT 
GCCCAGTTGG CTGGCTCTGT TGCTGAGATG TCGGCTTATC ATCATGGAGA ACAAGCATTG 
ATGATGACTA TTGTGAATTT GGCTCAGAAC TTTGTGGGAA GTAACAACAT TAACTTGCTT 
CAGCCTATTG GTCAGTTTGG AACTCGGCTT CATGGTGGCA AAGATGCTGC AAGCCCTCGT 
TATATTTTCA CAATGTTAAG CACTTTAGCA AGGCTACTTT TTCCTGCTGT GGATGACAAC 
CTCCTTAAGT TCCTTTATGA TGATAATCAA CGTGTAGAGC CTGAGTGGTA TATTCCTATA 
ATTCCCATGG TTTTAATAAA TGGTGCTGAG GGCATTGGTA CTGGATGGGC TTGTAAACTA 
CCCAACTATG ATGCTAGGGA AATTGTGAAC AATGTCAGAC GAATGCTAGA TGGCCTGGAT 
CCTCATCCCA TGCTTCCAAA CTACAAAAAC TTTAAAGGCA CGATTCAAGA ACTTGGTCAA 
AACCAGTATG CAGTCAGTGG TGAAATATTT GTAGTGGACA GAAACACAGT AGAAATTACA 
GAGCTTCCAG TTAGAACTTG GACACAGGTA TATAAAGAAC AGGTTTTAGA ACCTATGCTA 
AATGGAACAG ATAAAACACC AGCATTAATT TCTGATTATA AAGAATATCA TACTGACACA 
ACTGTGAAAT TTGTGGTGAA AATGACTGAA GAGAAACTAG CACAAGCAGA AGCTGCTGGA 
CTGCATAAAG TTTTTAAACT TCAAACTACT CTTACTTGTA ATTCCATGGT ACTTTTTGAT 
CATATGGGAT GTCTGAAGAA ATATGAAACT GTGCAAGACA TTCTGAAAGA ATTCTTTGAT 
TTACGATTAA GTTATTACGG TTTACGTAAG GAGTGGCTTG TGGGAATGTT GGGAGCAGAA 
TCTACAAAGC TTAACAATCA AGCCCGTTTC ATTTTAGAGA AGATACAAGG GAAAATTACT 
ATAGAGAATA GGTCAAAGAA AGATTTGATT CAAATGTTAG TCCAGAGAGG TTATGAATCT 
GACCCAGTGA AAGCCTGGAA AGAAGCACAA GAAAAGGCAG CAGAAGAGGA TGAAACACAA 
AACCAGCATG ATGATAGTTC CTCCGACTCA GGAACTCCTT CAGGCCCAGA TTTTAATTAT 
ATTTTAAATA TGTCTCTGTG GTCTCTTACT AAAGAAAAAG TTGAAGAACT GATTAAACAG 
AGAGATGCAA AAGGGCGAGA GGTCAATGAT CTTAAAAGAA AATCTCCTTC AGATCTTTGG 
AAAGAGGATT TAGCGGCATT TGTTGAAGAA CTGGATAAAG TGGAATCTCA AGAACGAGAA 
GATGTTCTGG CTGGAATGTC TGGAAAAGCA ATTAAAGGTA AAGTTGGCAA ACCTAAGGTG 
AAGAAACTCC AGTTGGAAGA GACAATGCCC TCACCTTATG GCAGAAGAAT AATTCCTGAA 
ATTACAGCTA TGAAGGCAGA TGCCAGCAAA AAGTTGCTGA AGAAGAAGAA GGGTGATCTT 
GATACTGCAG CAGTAAAAGT GGAATTTGAT GAAGAATTCA GTGGAGCGCC AGTAGAAGGT 
GCAGGAGAAG AGGCATTGAC TCCATCAGTT CCTATAAATA AAGGCCCCAA ACCCAAAAGG 
GAGAAGAAGG AGCCTGGTAC CAGAGTGAGA AAAACACCTA CATCATCTGG TAAACCTAGT 
GCAAAGAAAG TGAAGAAACG GAATCCTTGG TCAGATGATG AATCCAAGTC AGAAAGTGAT 
TTGGAAGAAA CAGAACCTGT GGTTATTCCA AGAGATTCTT TGCTTAGGAG AGCAGCAGCC 
GAAAGACCTA AATACACATT TGATTTCTCA GAAGAAGAGG ATGATGATGC TGATGATGAT 
GATGATGACA ATAATGATTT AGAGGAATTG AAAGTTAAAG CATCTCCCAT AACAAATGAT 
GGGGAAGATG AATTTGTTCC TTCAGATGGG TTAGATAAAG ATGAATATAC ATTTTCACCA 
GGCAAATCAA AAGCCACTCC AGAAAAATCT TTGCATGACA AAAAAAGTCA GGATTTTGGA 
AATCTCTTCT CATTTCCTTC ATATTCTCAG AAGTCAGAAG ATGATTCAGC TAAATTTGAC 
AGTAATGAAG AAGATTCTGC TTCTGTTTTT TCACCATCAT TTGGTCTGAA ACAGACAGAT 
AAAGTTCCAA GTAAAACGGT AGCTGCTAAA AAGGGAAAAC CGTCTTCAGA TACAGTCCCT 
AAGCCCAAGA GAGCCCCAAA ACAGAAGAAA GTAGTAGAGG CTGTAAACTC TGACTCGGAT 
TCAGAATTTG GCATTCCAAA GAAGACTACA ACACCAAAAG GTAAAGGCCG AGGGGCAAAG 
AAAAGGAAAG CATCTGGCTC TGAAAATGAA GGCGATTATA ACCCTGGCAG GAAAACATCC 
AAAACAACAA GCAAGAAACC GAAGAAGACA TCTTTTGATC AGGATTCAGA TGTGGACATC 
TTCCCCTCAG ACTTCCCTAC TGAGCCACCT TCTCTGCCAC GAACCGGTCG GGCTAGGAAA 
GAAGTAAAAT ATTTTGCAGA GTCTGATGAA GAAGATGATG ATGTTGATTT TGCAATGTTT 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_016940585.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016796074.1 Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA.

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CloneID	OPc81502
Clone ID Related Accession (Same CDS sequence)	XM_016940585.1
Accession Version	XM_016940585.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	DNA topoisomerase 2-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TTATAAGCAT TTGGAATAAT 
GGGAAAGGCA TTCCAGTAGT AGAACACAAG GTAGAGAAAG TTTATGTTCC TGCTTTAATT 
TTTGGACAGC TTTTAACATC CAGTAACTAT GATGATGATG AGAAAAAAGT TACAGGTGGT 
CGTAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACA 
GCTTGCAAAG AATACAAACA CAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGCATAAC ATTCCAACCA 
GATCTGTCCA AATTTAAGAT GGAAAAACTT GACAAGGATA TTGTGGCCCT CATGACTAGA 
AGGGCATATG ATTTGGCTGG TTCGTGTAGA GGGGTCAAGG TCATGTTTAA TGGAAAGAAA 
TTGCCTGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
ACTGGGGTGG CCCTGAAAGT TATTCATGAG CTTGCAAATG AAAGATGGGA TGTTTGTCTC 
ACATTGAGTG AAAAAGGATT CCAGCAAATC AGTTTTGTAA ATAGTATTGC AACTACAAAA 
GGTGGACGGC ACGTGGATTA TGTGGTAGAT CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
AAGAAAAAGA ACAAAGCTGG TGTATCAGTG AAACCATTTC AAGTAAAAAA CCATATATGG 
GTTTTTATTA ATTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAGACTAA GGAAAACATG 
ACTCTGCAGC CCAAAAGTTT TGGGTCTAAA TGCCAGCTGT CAGAAAAATT TTTTAAAGCA 
GCCTCTAATT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACT 
CAGCTGAATA AGAAGTGTTC ATCAGTAAAA TACAGTAAAA TCAAAGGTAT TCCCAAACTG 
GATGATGCTA ATGATGCTGG TGGTAAACAT TCCCTGGAGT GTACACTGAT ATTAACAGAG 
GGAGACTCTG CCAAATCACT GGCTGTGTCT GGATTAGGTG TGATTGGACG AGACAGATAT 
GGAGTTTTTC CACTCAGGGG CAAAATTCTT AATGTACGGG AAGCTTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATAAA TAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
TACGATGATG CAGAATCTCT GAAAACCTTA CGCTATGGAA AGATTATGAT TATGACCGAT 
CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
CCATCACTTT TGAAGCATGG TTTTCTCGAA GAGTTCATTA CTCCTATTGT AAAGGCAAGC 
AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGACGAATG GAAAAAACAT 
ATAGAAAACC AGAAAGCCTG GAAAATAAAG TACTATAAAG GATTGGGTAC TAGTACAGCT 
AAAGAAGCAA AGGAATATTT TGCTGATATG GAAAGGCATC GCATCTTGTT TAGATATGCT 
GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTTAGTA AGAAGAAGAT TGATGACAGA 
AAAGAATGGT TAACAAATTT TATGGAAGAC CGGAGACAGC GTAGGCTACA TGGCTTACCA 
GAGCAATTTT TATATGGTAC TGCAACAAAG CATTTGACTT ATAATGATTT CATCAACAAG 
GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGC 
TTTAAACCTG GCCAGCGGAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
GTAAAAGTTG CCCAGTTGGC TGGCTCTGTT GCTGAGATGT CGGCTTATCA TCATGGAGAA 
CAAGCATTGA TGATGACTAT TGTGAATTTG GCTCAGAACT TTGTGGGAAG TAACAACATT 
AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGGCTTC ATGGTGGCAA AGATGCTGCA 
AGCCCTCGTT ATATTTTCAC AATGTTAAGC ACTTTAGCAA GGCTACTTTT TCCTGCTGTG 
GATGACAACC TCCTTAAGTT CCTTTATGAT GATAATCAAC GTGTAGAGCC TGAGTGGTAT 
ATTCCTATAA TTCCCATGGT TTTAATAAAT GGTGCTGAGG GCATTGGTAC TGGATGGGCT 
TGTAAACTAC CCAACTATGA TGCTAGGGAA ATTGTGAACA ATGTCAGACG AATGCTAGAT 
GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGCAC GATTCAAGAA 
CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAAATATTTG TAGTGGACAG AAACACAGTA 
GAAATTACAG AGCTTCCAGT TAGAACTTGG ACACAGGTAT ATAAAGAACA GGTTTTAGAA 
CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
ACTGACACAA CTGTGAAATT TGTGGTGAAA ATGACTGAAG AGAAACTAGC ACAAGCAGAA 
GCTGCTGGAC TGCATAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
CTTTTTGATC ATATGGGATG TCTGAAGAAA TATGAAACTG TGCAAGACAT TCTGAAAGAA 
TTCTTTGATT TACGATTAAG TTATTACGGT TTACGTAAGG AGTGGCTTGT GGGAATGTTG 
GGAGCAGAAT CTACAAAGCT TAACAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
AAAATTACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAT 
GAAACACAAA ACCAGCATGA TGATAGTTCC TCCGACTCAG GAACTCCTTC AGGCCCAGAT 
TTTAATTATA TTTTAAATAT GTCTCTGTGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAATGATC TTAAAAGAAA ATCTCCTTCA 
GATCTTTGGA AAGAGGATTT AGCGGCATTT GTTGAAGAAC TGGATAAAGT GGAATCTCAA 
GAACGAGAAG ATGTTCTGGC TGGAATGTCT GGAAAAGCAA TTAAAGGTAA AGTTGGCAAA 
CCTAAGGTGA AGAAACTCCA GTTGGAAGAG ACAATGCCCT CACCTTATGG CAGAAGAATA 
ATTCCTGAAA TTACAGCTAT GAAGGCAGAT GCCAGCAAAA AGTTGCTGAA GAAGAAGAAG 
GGTGATCTTG ATACTGCAGC AGTAAAAGTG GAATTTGATG AAGAATTCAG TGGAGCGCCA 
GTAGAAGGTG CAGGAGAAGA GGCATTGACT CCATCAGTTC CTATAAATAA AGGCCCCAAA 
CCCAAAAGGG AGAAGAAGGA GCCTGGTACC AGAGTGAGAA AAACACCTAC ATCATCTGGT 
AAACCTAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
GAAAGTGATT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGATTCTTT GCTTAGGAGA 
GCAGCAGCCG AAAGACCTAA ATACACATTT GATTTCTCAG AAGAAGAGGA TGATGATGCT 
GATGATGATG ATGATGACAA TAATGATTTA GAGGAATTGA AAGTTAAAGC ATCTCCCATA 
ACAAATGATG GGGAAGATGA ATTTGTTCCT TCAGATGGGT TAGATAAAGA TGAATATACA 
TTTTCACCAG GCAAATCAAA AGCCACTCCA GAAAAATCTT TGCATGACAA AAAAAGTCAG 
GATTTTGGAA ATCTCTTCTC ATTTCCTTCA TATTCTCAGA AGTCAGAAGA TGATTCAGCT 
AAATTTGACA GTAATGAAGA AGATTCTGCT TCTGTTTTTT CACCATCATT TGGTCTGAAA 
CAGACAGATA AAGTTCCAAG TAAAACGGTA GCTGCTAAAA AGGGAAAACC GTCTTCAGAT 
ACAGTCCCTA AGCCCAAGAG AGCCCCAAAA CAGAAGAAAG TAGTAGAGGC TGTAAACTCT 
GACTCGGATT CAGAATTTGG CATTCCAAAG AAGACTACAA CACCAAAAGG TAAAGGCCGA 
GGGGCAAAGA AAAGGAAAGC ATCTGGCTCT GAAAATGAAG GCGATTATAA CCCTGGCAGG 
AAAACATCCA AAACAACAAG CAAGAAACCG AAGAAGACAT CTTTTGATCA GGATTCAGAT 
GTGGACATCT TCCCCTCAGA CTTCCCTACT GAGCCACCTT CTCTGCCACG AACCGGTCGG 
GCTAGGAAAG AAGTAAAATA TTTTGCAGAG TCTGATGAAG AAGATGATGA TGTTGATTTT 
GCAATGTTTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016796074.1
CDS	553..5067
Translation	MTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLDKDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFSPSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEPPSLPRTGRARKEVKYFAESDEEDDDVDFAMFN

Target ORF information:

RefSeq Version	XM_016940585.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016940585.1

ORF Insert Sequence:

ATGACTTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TTATAAGCAT TTGGAATAAT 
GGGAAAGGCA TTCCAGTAGT AGAACACAAG GTAGAGAAAG TTTATGTTCC TGCTTTAATT 
TTTGGACAGC TTTTAACATC CAGTAACTAT GATGATGATG AGAAAAAAGT TACAGGTGGT 
CGTAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACA 
GCTTGCAAAG AATACAAACA CAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGCATAAC ATTCCAACCA 
GATCTGTCCA AATTTAAGAT GGAAAAACTT GACAAGGATA TTGTGGCCCT CATGACTAGA 
AGGGCATATG ATTTGGCTGG TTCGTGTAGA GGGGTCAAGG TCATGTTTAA TGGAAAGAAA 
TTGCCTGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
ACTGGGGTGG CCCTGAAAGT TATTCATGAG CTTGCAAATG AAAGATGGGA TGTTTGTCTC 
ACATTGAGTG AAAAAGGATT CCAGCAAATC AGTTTTGTAA ATAGTATTGC AACTACAAAA 
GGTGGACGGC ACGTGGATTA TGTGGTAGAT CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
AAGAAAAAGA ACAAAGCTGG TGTATCAGTG AAACCATTTC AAGTAAAAAA CCATATATGG 
GTTTTTATTA ATTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAGACTAA GGAAAACATG 
ACTCTGCAGC CCAAAAGTTT TGGGTCTAAA TGCCAGCTGT CAGAAAAATT TTTTAAAGCA 
GCCTCTAATT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACT 
CAGCTGAATA AGAAGTGTTC ATCAGTAAAA TACAGTAAAA TCAAAGGTAT TCCCAAACTG 
GATGATGCTA ATGATGCTGG TGGTAAACAT TCCCTGGAGT GTACACTGAT ATTAACAGAG 
GGAGACTCTG CCAAATCACT GGCTGTGTCT GGATTAGGTG TGATTGGACG AGACAGATAT 
GGAGTTTTTC CACTCAGGGG CAAAATTCTT AATGTACGGG AAGCTTCTCA TAAACAGATC 
ATGGAAAATG CTGAAATAAA TAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
TACGATGATG CAGAATCTCT GAAAACCTTA CGCTATGGAA AGATTATGAT TATGACCGAT 
CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
CCATCACTTT TGAAGCATGG TTTTCTCGAA GAGTTCATTA CTCCTATTGT AAAGGCAAGC 
AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGACGAATG GAAAAAACAT 
ATAGAAAACC AGAAAGCCTG GAAAATAAAG TACTATAAAG GATTGGGTAC TAGTACAGCT 
AAAGAAGCAA AGGAATATTT TGCTGATATG GAAAGGCATC GCATCTTGTT TAGATATGCT 
GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTTAGTA AGAAGAAGAT TGATGACAGA 
AAAGAATGGT TAACAAATTT TATGGAAGAC CGGAGACAGC GTAGGCTACA TGGCTTACCA 
GAGCAATTTT TATATGGTAC TGCAACAAAG CATTTGACTT ATAATGATTT CATCAACAAG 
GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGC 
TTTAAACCTG GCCAGCGGAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
GTAAAAGTTG CCCAGTTGGC TGGCTCTGTT GCTGAGATGT CGGCTTATCA TCATGGAGAA 
CAAGCATTGA TGATGACTAT TGTGAATTTG GCTCAGAACT TTGTGGGAAG TAACAACATT 
AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGGCTTC ATGGTGGCAA AGATGCTGCA 
AGCCCTCGTT ATATTTTCAC AATGTTAAGC ACTTTAGCAA GGCTACTTTT TCCTGCTGTG 
GATGACAACC TCCTTAAGTT CCTTTATGAT GATAATCAAC GTGTAGAGCC TGAGTGGTAT 
ATTCCTATAA TTCCCATGGT TTTAATAAAT GGTGCTGAGG GCATTGGTAC TGGATGGGCT 
TGTAAACTAC CCAACTATGA TGCTAGGGAA ATTGTGAACA ATGTCAGACG AATGCTAGAT 
GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGCAC GATTCAAGAA 
CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAAATATTTG TAGTGGACAG AAACACAGTA 
GAAATTACAG AGCTTCCAGT TAGAACTTGG ACACAGGTAT ATAAAGAACA GGTTTTAGAA 
CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
ACTGACACAA CTGTGAAATT TGTGGTGAAA ATGACTGAAG AGAAACTAGC ACAAGCAGAA 
GCTGCTGGAC TGCATAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
CTTTTTGATC ATATGGGATG TCTGAAGAAA TATGAAACTG TGCAAGACAT TCTGAAAGAA 
TTCTTTGATT TACGATTAAG TTATTACGGT TTACGTAAGG AGTGGCTTGT GGGAATGTTG 
GGAGCAGAAT CTACAAAGCT TAACAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
AAAATTACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAT 
GAAACACAAA ACCAGCATGA TGATAGTTCC TCCGACTCAG GAACTCCTTC AGGCCCAGAT 
TTTAATTATA TTTTAAATAT GTCTCTGTGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAATGATC TTAAAAGAAA ATCTCCTTCA 
GATCTTTGGA AAGAGGATTT AGCGGCATTT GTTGAAGAAC TGGATAAAGT GGAATCTCAA 
GAACGAGAAG ATGTTCTGGC TGGAATGTCT GGAAAAGCAA TTAAAGGTAA AGTTGGCAAA 
CCTAAGGTGA AGAAACTCCA GTTGGAAGAG ACAATGCCCT CACCTTATGG CAGAAGAATA 
ATTCCTGAAA TTACAGCTAT GAAGGCAGAT GCCAGCAAAA AGTTGCTGAA GAAGAAGAAG 
GGTGATCTTG ATACTGCAGC AGTAAAAGTG GAATTTGATG AAGAATTCAG TGGAGCGCCA 
GTAGAAGGTG CAGGAGAAGA GGCATTGACT CCATCAGTTC CTATAAATAA AGGCCCCAAA 
CCCAAAAGGG AGAAGAAGGA GCCTGGTACC AGAGTGAGAA AAACACCTAC ATCATCTGGT 
AAACCTAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
GAAAGTGATT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGATTCTTT GCTTAGGAGA 
GCAGCAGCCG AAAGACCTAA ATACACATTT GATTTCTCAG AAGAAGAGGA TGATGATGCT 
GATGATGATG ATGATGACAA TAATGATTTA GAGGAATTGA AAGTTAAAGC ATCTCCCATA 
ACAAATGATG GGGAAGATGA ATTTGTTCCT TCAGATGGGT TAGATAAAGA TGAATATACA 
TTTTCACCAG GCAAATCAAA AGCCACTCCA GAAAAATCTT TGCATGACAA AAAAAGTCAG 
GATTTTGGAA ATCTCTTCTC ATTTCCTTCA TATTCTCAGA AGTCAGAAGA TGATTCAGCT 
AAATTTGACA GTAATGAAGA AGATTCTGCT TCTGTTTTTT CACCATCATT TGGTCTGAAA 
CAGACAGATA AAGTTCCAAG TAAAACGGTA GCTGCTAAAA AGGGAAAACC GTCTTCAGAT 
ACAGTCCCTA AGCCCAAGAG AGCCCCAAAA CAGAAGAAAG TAGTAGAGGC TGTAAACTCT 
GACTCGGATT CAGAATTTGG CATTCCAAAG AAGACTACAA CACCAAAAGG TAAAGGCCGA 
GGGGCAAAGA AAAGGAAAGC ATCTGGCTCT GAAAATGAAG GCGATTATAA CCCTGGCAGG 
AAAACATCCA AAACAACAAG CAAGAAACCG AAGAAGACAT CTTTTGATCA GGATTCAGAT 
GTGGACATCT TCCCCTCAGA CTTCCCTACT GAGCCACCTT CTCTGCCACG AACCGGTCGG 
GCTAGGAAAG AAGTAAAATA TTTTGCAGAG TCTGATGAAG AAGATGATGA TGTTGATTTT 
GCAATGTTTA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

TOP2B cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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