Top2b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top2b
Entrez Gene ID	21974
Full Name	topoisomerase (DNA) II beta
Synonyms	D230016L12Rik,Top-2
General protein information	Preferred Names topoisomerase (DNA) II beta Names DNA topoisomerase 2-beta DNA topoisomerase II, beta isozyme
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 A2

mRNA and Protein(s)

mRNA	Protein	Name
NM_009409.2	NP_033435.2	DNA topoisomerase 2-beta

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes.
Yu C, Fan X, Sha QQ, Wang HH, Li BT, Dai XX, Shen L, Liu J, Wang L, Liu K, Tang F, Fan HY
Cell reports20(5)1161-1172(2017 Aug)

Genome Organization Drives Chromosome Fragility.
Canela A, Maman Y, Jung S, Wong N, Callen E, Day A, Kieffer-Kwon KR, Pekowska A, Zhang H, Rao SSP, Huang SC, Mckinnon PJ, Aplan PD, Pommier Y, Aiden EL, Casellas R, Nussenzweig A
Cell170(3)507-521.e18(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We demonstrated that Nup98-TopIIbeta and Nup98-SETBP1 negatively regulate the XPO1-mediated protein export. Our results will contribute to the understanding of the molecular mechanism by which the Nup98-fusion proteins induce tumorigenesis.
Title: Function of Nup98 subtypes and their fusion proteins, Nup98-TopII? and Nup98-SETBP1 in nuclear-cytoplasmic transport.

TOP2beta as a factor involved in regulating granulosa cell genomic integrity and follicle atresia.
Title: TOP2? is essential for ovarian follicles that are hypersensitive to chemotherapeutic drugs.

Results identified topoisomerase IIbeta (Top2beta) as a novel determinant for camptothecin sensitivity.
Title: Topoisomerase II? deficiency enhances camptothecin-induced apoptosis.

Findings suggest a chromatin-based targeting of Top2beta to regulatory regions in the genome to govern the transcriptional program associated with neuronal differentiation and longevity.
Title: Target genes of Topoisomerase II? regulate neuronal survival and are defined by their chromatin state.

Top IIbeta might be a down-stream target of Nurr1, which might influence the processes of axon genesis in dopaminergic neurons via the regulation of TOP IIbeta expression.
Title: Nurr1 regulates Top II? and functions in axon genesis of mesencephalic dopaminergic neurons.

The following Top2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19140	NM_009409.2 Latest version!	Mus musculus topoisomerase (DNA) II beta (Top2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	22	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top2b ( NM_009409.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033435.2 Mus musculus topoisomerase (DNA) II beta (Top2b), mRNA.

Price & Availability↑

CloneID	OMu19140
Clone ID Related Accession (Same CDS sequence)	NM_009409.2
Accession Version	NM_009409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Mus musculus(house mouse)
Product	DNA topoisomerase 2-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054541.1. On Aug 28, 2003 this sequence version replaced NM_009409.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC054541.1, BC041106.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGT CCAGCCTCGC GGGCTCTGAC GGGGCGCTGA CCTGGGTGAA CAATGCTACA 
AAAAAGGAAG AGTTGGAGAC AGCAAACAAA AATGACTCTA CTAAGAAGTT GTCTGTTGAG 
AGAGTGTACC AGAAGAAGAC CCAACTGGAG CACATTCTCC TTCGTCCTGA TACATACATT 
GGATCAGTGG AGCCACTGAC ACAGCTAATG TGGGTGTATG ATGAAGATGT AGGGATGAAC 
TGCAGGGAGG TCACCTTTGT TCCAGGCTTA TACAAGATCT TTGATGAAAT TTTGGTTAAT 
GCTGCTGACA ACAAGCAGAG GGACAAGAAC ATGACCTGTA TTAAGGTTTC TATTGATCCT 
GAATCTAATA TTATAAGCAT TTGGAATAAT GGGAAAGGCA TTCCAGTAGT AGAACACAAA 
GTAGAGAAAG TTTATGTTCC TGCTTTAATT TTTGGACAGC TTTTAACATC GAGTAATTAT 
GACGATGATG AGAAAAAAGT TACAGGAGGC CGTAATGGTT ATGGTGCAAA GCTTTGTAAT 
ATTTTTAGTA CAAAGTTTAC AGTAGAAACG GCTTGCAAAG AATACAAACA TAGTTTTAAA 
CAGACATGGA TGAATAATAT GATGAAGACT TCTGAAGCCA AAATTAAACA TTTTGATGGT 
GAAGATTATA CATGCATAAC ATTCCAGCCA GATCTATCCA AATTTAAGAT GGAAAAACTT 
GACAAGGACA TTGTGGCCCT CATGACCAGA AGAGCATATG ATTTAGCTGG GTCCTGTAAA 
GGAGTCAAGG TCATGTTTAA TGGGAAGAAG TTGCCTGTAA ATGGATTTCG CAGCTATGTA 
GACCTTTATG TGAAAGACAA ATTGGATGAA ACTGGGGTGG CTCTCAAAGT TATTCATGAA 
CTTGCAAATG AAAGATGGGA TGTTTGTCTA ACCTTGAGTG AAAAAGGTTT CCAGCAGATC 
AGCTTTGTGA ATAGTATTGC AACTACAAAA GGTGGGCGGC ATGTGGATTA TGTAGTAGAT 
CAAGTTGTTA GTAAACTGAT TGAAGTAGTT AAGAAAAAGA ACAAAGCTGG TGTATCAGTG 
AAACCATTTC AGGTTAAAAA CCATATATGG GTTTTTATTA ATTGTCTTAT TGAAAATCCA 
ACCTTTGATT CTCAAACTAA GGAAAACATG ACTCTGCAGC CCAAAAGTTT TGGGTCCAAA 
TGCCAACTGT CAGAAAAATT TTTTAAAGCA GCCTCTAATT GTGGCATTGT AGAAAGCATA 
TTGAACTGGG TGAAATTTAA GGCTCAGACT CAACTGAATA AGAAGTGTTC ATCAGTAAAG 
TACAGTAAAA TCAAAGGCAT TCCCAAACTG GATGATGCCA ATGATGCTGG TGGCAAACAT 
TCCCTGGAGT GTACATTAAT ATTAACAGAG GGGGACTCCG CCAAATCTCT AGCTGTATCT 
GGATTAGGTG TGATTGGTAG GGACAGATAT GGAGTCTTCC CACTCAGGGG TAAAATTCTC 
AATGTACGAG AAGCCTCTCA TAAACAGATT ATGGAAAATG CAGAGATAAA TAATATTATT 
AAAATAGTTG GTTTGCAATA CAAGAAAAGT TATGATGATG CAGAATCTCT GAAAACTTTA 
CGTTATGGAA AGATTATGAT TATGACTGAT CAGGATCAAG ATGGTTCTCA CATCAAAGGC 
CTGCTAATCA ATTTTATTCA TCACAATTGG CCATCACTTT TGAAGCATGG TTTTCTTGAA 
GAATTCATTA CTCCCATTGT AAAGGCAAGC AAAAATAAGC AGGAACTTTC CTTTTATAGT 
ATTCCTGAAT TTGATGAGTG GAAAAAACAT ATAGAAAACC AGAAAGCCTG GAAAATAAAG 
TACTACAAAG GATTAGGTAC CAGTACCGCT AAAGAAGCAA AGGAATATTT TGCTGATATG 
GAAAGGCATC GCATTTTGTT TAGATATGCT GGACCTGAAG ATGATGCTGC CATCACCTTG 
GCATTTAGTA AGAAGAAGAT TGATGACAGA AAAGAGTGGT TAACCAACTT TATGGAGGAT 
CGGAGACAGC GTAGGTTACA TGGCCTACCA GAGCAATTTT TATATGGTAC AGCAACAAAG 
CATTTGACAT ACAATGATTT CATCAACAAG GAATTGATTC TCTTCTCAAA CTCGGACAAT 
GAGAGATCTA TCCCATCTCT TGTGGATGGC TTCAAGCCAG GTCAACGAAA AGTTTTGTTT 
ACCTGTTTCA AGAGGAATGA TAAACGTGAA GTGAAAGTAG CGCAGCTGGC TGGATCTGTT 
GCTGAGATGT CTGCTTACCA CCATGGAGAA CAAGCATTGA TGATGACTAT TGTGAATTTG 
GCTCAGAATT TTGTGGGCAG TAATAACATT AACTTGCTTC AGCCCATTGG TCAGTTTGGA 
ACTCGGCTTC ATGGTGGCAA AGATGCTGCA AGCCCTCGTT ATATTTTCAC AATGTTGAGC 
TCTCTGGCAA GACTTCTTTT TCCAGCTGTG GATGATAACT TGCTTAAGTT CCTTTATGAT 
GATAATCAAC GTGTAGAGCC AGAGTGGTAT ATCCCTATAA TCCCCATGGT TTTGATTAAT 
GGTGCTGAGG GTATTGGGAC TGGATGGGCT TGTAAACTGC CCAACTATGA TGCTAGAGAA 
ATTGTGAACA ATGTCAGGCG GATGCTAGAA GGTTTGGATC CTCATCCCAT GCTTCCAAAC 
TATAAGAACT TCAAAGGCAC CATCCAGGAA CTTGGTCAGA ACCAGTATGC TGTTAGTGGT 
GAGATATTTG TGGTGGACAG AAACACAGTA GAGATTACAG AACTTCCAGT TAGAACATGG 
ACACAGGTAT ACAAGGAGCA GGTCCTAGAA CCTATGCTTA ATGGAACAGA TAAAACACCG 
GCACTGATTT CTGATTACAA AGAATATCAT ACTGACACAA CTGTGAAGTT TGTGGTGAAA 
ATGACAGAAG AGAAACTAGC GCAAGCAGAA GCTGCTGGCC TGCATAAAGT TTTTAAACTT 
CAAACTACTC TTACTTGCAA TTCCATGGTA CTCTTTGATC ATATGGGTTG TCTGAAGAAG 
TATGAAACTG TGCAAGACAT TTTGAAAGAA TTCTTTGATT TACGGTTAAG TTATTATGGT 
TTACGGAAGG AATGGCTTGT AGGAATGCTG GGAGCAGAAT CTACAAAGCT TAATAATCAA 
GCCCGTTTCA TTTTAGAGAA GATACAGGGG AAAATTACTA TAGAGAATAG GTCAAAGAAA 
GATTTGATTC AAATGTTAGT CCAGAGAGGT TATGAATCTG ACCCAGTGAA AGCGTGGAAA 
GAAGCACAAG AAAAGGCTGC AGAAGAGGAA GATTCACAAA ACCAACATGA TGACAGTTCC 
TCTGATTCAG GAACTCCTTC AGGCCCTGAT TTTAATTATA TTTTAAATAT GTCTCTATGG 
TCTCTTACTA AAGAAAAAGT TGAAGAACTC ATTAAACAGA GAGATACGAA AGGGCGAGAG 
GTTAATGATC TCAAGAGGAA GTCTCCTTCA GATCTTTGGA AAGAAGACTT AGCAGCATTT 
GTTGAAGAAT TGGATAAAGT GGAAGCACAA GAGCGGGAAG ATATTCTGGC AGGAATGTCT 
GGGAAAGCAA TAAAAGGTAA AGTCGGCAAA CCCAAAGTAA AGAAACTTCA GCTGGAGGAG 
ACAATGCCCT CGCCTTACGG GAGAAGAATT GTTCCAGAGA TTACTGCTAT GAAGGCAGAT 
GCCAGCAGAA AATTGCTGAA GAAGAAAAAG GGTGATCCTG ATACCACAGT AGTGAAAGTA 
GAATTTGATG AAGAATTTAG TGGAACACCA GCAGAAGGTA CAGGAGAGGA GACATTGACC 
CCATCTGCTC CTGTAAATAA AGGCCCCAAA CCCAAAAGAG AGAAGAAGGA GCCTGGTACC 
AGGGTTAGAA AAACACCTAC ATCAACTGGT AAAACTAATG CGAAGAAAGT GAAGAAACGG 
AATCCTTGGT CAGATGATGA GTCCAAATCA GAGAGTGACT TGGAAGAAGC AGAACCTGTG 
GTTATCCCAA GAGATTCTTT GCTTAGGAGA GCAGCTGCTG AAAGACCTAA GTACACTTTT 
GATTTCTCAG AAGAGGAGGA TGATGATGCT GCTGCTGCTG ATGACAGTAA TGATTTAGAA 
GAACTGAAAG TTAAAGCATC TCCCATAACA AATGATGGGG AAGATGAATT TGTTCCTTCA 
GATGGCTTAG ATAAAGATGA GTATGCTTTT TCTTCAGGCA AATCCAAAGC TACTCCAGAA 
AAATCTTCAA ATGACAAAAA AAGTCAGGAT TTTGGAAATC TTTTTTCATT TCCTTCATAT 
TCTCAGAAGT CAGAAGATGA TTCAGCTAAA TTTGACAGTA ATGAAGAAGA TACCGCTTCT 
GTTTTTGCTC CATCATTTGG TCTGAAGCAA ACAGATAAAC TTCCAAGTAA AACAGTTGCT 
GCTAAAAAGG GTAAACCACC TTCAGATACA GCCCCTAAAG CTAAGAGAGC CCCTAAACAG 
AAGAAGATAG TAGAGACTAT AAACTCTGAC TCAGACTCTG AATTTGGCAT TCCAAAGAAG 
ACTACAACAC CAAAAGGTAA AGGCCGAGGG GCAAAGAAAA GGAAAGCATC AGGCTCAGAA 
AATGAAGGTG ATTATAACCC TGGAAGGAAA CCATCTAAAA CAGCCAGCAA GAAACCGAAG 
AAAACATCTT TTGATCAGGA TTCAGATGTA GACATCTTCC CCTCAGACTT CACTTCTGAA 
CCACCGGCTC TCCCACGGAC TGGTCGGGCT AGGAAAGAAG TAAAATATTT TGCAGAGTCT 
GATGAAGAAG AAGATGTTGA TTTTGCAATG TTTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033435.2
CDS	474..5312
Translation	MAKSSLAGSDGALTWVNNATKKEELETANKNDSTKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQEREDILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEITAMKADASRKLLKKKKGDPDTTVVKVEFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKRNPWSDDESKSESDLEEAEPVVIPRDSLLRRAAAERPKYTFDFSEEEDDDAAAADDSNDLEELKVKASPITNDGEDEFVPSDGLDKDEYAFSSGKSKATPEKSSNDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDTASVFAPSFGLKQTDKLPSKTVAAKKGKPPSDTAPKAKRAPKQKKIVETINSDSDSEFGIPKKTTTPKGKGRGAKKRKASGSENEGDYNPGRKPSKTASKKPKKTSFDQDSDVDIFPSDFTSEPPALPRTGRARKEVKYFAESDEEEDVDFAMFN

Target ORF information:

RefSeq Version	NM_009409.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus topoisomerase (DNA) II beta (Top2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009409.2

ORF Insert Sequence:

ATGGCCAAGT CCAGCCTCGC GGGCTCTGAC GGGGCGCTGA CCTGGGTGAA CAATGCTACA 
AAAAAGGAAG AGTTGGAGAC AGCAAACAAA AATGACTCTA CTAAGAAGTT GTCTGTTGAG 
AGAGTGTACC AGAAGAAGAC CCAACTGGAG CACATTCTCC TTCGTCCTGA TACATACATT 
GGATCAGTGG AGCCACTGAC ACAGCTAATG TGGGTGTATG ATGAAGATGT AGGGATGAAC 
TGCAGGGAGG TCACCTTTGT TCCAGGCTTA TACAAGATCT TTGATGAAAT TTTGGTTAAT 
GCTGCTGACA ACAAGCAGAG GGACAAGAAC ATGACCTGTA TTAAGGTTTC TATTGATCCT 
GAATCTAATA TTATAAGCAT TTGGAATAAT GGGAAAGGCA TTCCAGTAGT AGAACACAAA 
GTAGAGAAAG TTTATGTTCC TGCTTTAATT TTTGGACAGC TTTTAACATC GAGTAATTAT 
GACGATGATG AGAAAAAAGT TACAGGAGGC CGTAATGGTT ATGGTGCAAA GCTTTGTAAT 
ATTTTTAGTA CAAAGTTTAC AGTAGAAACG GCTTGCAAAG AATACAAACA TAGTTTTAAA 
CAGACATGGA TGAATAATAT GATGAAGACT TCTGAAGCCA AAATTAAACA TTTTGATGGT 
GAAGATTATA CATGCATAAC ATTCCAGCCA GATCTATCCA AATTTAAGAT GGAAAAACTT 
GACAAGGACA TTGTGGCCCT CATGACCAGA AGAGCATATG ATTTAGCTGG GTCCTGTAAA 
GGAGTCAAGG TCATGTTTAA TGGGAAGAAG TTGCCTGTAA ATGGATTTCG CAGCTATGTA 
GACCTTTATG TGAAAGACAA ATTGGATGAA ACTGGGGTGG CTCTCAAAGT TATTCATGAA 
CTTGCAAATG AAAGATGGGA TGTTTGTCTA ACCTTGAGTG AAAAAGGTTT CCAGCAGATC 
AGCTTTGTGA ATAGTATTGC AACTACAAAA GGTGGGCGGC ATGTGGATTA TGTAGTAGAT 
CAAGTTGTTA GTAAACTGAT TGAAGTAGTT AAGAAAAAGA ACAAAGCTGG TGTATCAGTG 
AAACCATTTC AGGTTAAAAA CCATATATGG GTTTTTATTA ATTGTCTTAT TGAAAATCCA 
ACCTTTGATT CTCAAACTAA GGAAAACATG ACTCTGCAGC CCAAAAGTTT TGGGTCCAAA 
TGCCAACTGT CAGAAAAATT TTTTAAAGCA GCCTCTAATT GTGGCATTGT AGAAAGCATA 
TTGAACTGGG TGAAATTTAA GGCTCAGACT CAACTGAATA AGAAGTGTTC ATCAGTAAAG 
TACAGTAAAA TCAAAGGCAT TCCCAAACTG GATGATGCCA ATGATGCTGG TGGCAAACAT 
TCCCTGGAGT GTACATTAAT ATTAACAGAG GGGGACTCCG CCAAATCTCT AGCTGTATCT 
GGATTAGGTG TGATTGGTAG GGACAGATAT GGAGTCTTCC CACTCAGGGG TAAAATTCTC 
AATGTACGAG AAGCCTCTCA TAAACAGATT ATGGAAAATG CAGAGATAAA TAATATTATT 
AAAATAGTTG GTTTGCAATA CAAGAAAAGT TATGATGATG CAGAATCTCT GAAAACTTTA 
CGTTATGGAA AGATTATGAT TATGACTGAT CAGGATCAAG ATGGTTCTCA CATCAAAGGC 
CTGCTAATCA ATTTTATTCA TCACAATTGG CCATCACTTT TGAAGCATGG TTTTCTTGAA 
GAATTCATTA CTCCCATTGT AAAGGCAAGC AAAAATAAGC AGGAACTTTC CTTTTATAGT 
ATTCCTGAAT TTGATGAGTG GAAAAAACAT ATAGAAAACC AGAAAGCCTG GAAAATAAAG 
TACTACAAAG GATTAGGTAC CAGTACCGCT AAAGAAGCAA AGGAATATTT TGCTGATATG 
GAAAGGCATC GCATTTTGTT TAGATATGCT GGACCTGAAG ATGATGCTGC CATCACCTTG 
GCATTTAGTA AGAAGAAGAT TGATGACAGA AAAGAGTGGT TAACCAACTT TATGGAGGAT 
CGGAGACAGC GTAGGTTACA TGGCCTACCA GAGCAATTTT TATATGGTAC AGCAACAAAG 
CATTTGACAT ACAATGATTT CATCAACAAG GAATTGATTC TCTTCTCAAA CTCGGACAAT 
GAGAGATCTA TCCCATCTCT TGTGGATGGC TTCAAGCCAG GTCAACGAAA AGTTTTGTTT 
ACCTGTTTCA AGAGGAATGA TAAACGTGAA GTGAAAGTAG CGCAGCTGGC TGGATCTGTT 
GCTGAGATGT CTGCTTACCA CCATGGAGAA CAAGCATTGA TGATGACTAT TGTGAATTTG 
GCTCAGAATT TTGTGGGCAG TAATAACATT AACTTGCTTC AGCCCATTGG TCAGTTTGGA 
ACTCGGCTTC ATGGTGGCAA AGATGCTGCA AGCCCTCGTT ATATTTTCAC AATGTTGAGC 
TCTCTGGCAA GACTTCTTTT TCCAGCTGTG GATGATAACT TGCTTAAGTT CCTTTATGAT 
GATAATCAAC GTGTAGAGCC AGAGTGGTAT ATCCCTATAA TCCCCATGGT TTTGATTAAT 
GGTGCTGAGG GTATTGGGAC TGGATGGGCT TGTAAACTGC CCAACTATGA TGCTAGAGAA 
ATTGTGAACA ATGTCAGGCG GATGCTAGAA GGTTTGGATC CTCATCCCAT GCTTCCAAAC 
TATAAGAACT TCAAAGGCAC CATCCAGGAA CTTGGTCAGA ACCAGTATGC TGTTAGTGGT 
GAGATATTTG TGGTGGACAG AAACACAGTA GAGATTACAG AACTTCCAGT TAGAACATGG 
ACACAGGTAT ACAAGGAGCA GGTCCTAGAA CCTATGCTTA ATGGAACAGA TAAAACACCG 
GCACTGATTT CTGATTACAA AGAATATCAT ACTGACACAA CTGTGAAGTT TGTGGTGAAA 
ATGACAGAAG AGAAACTAGC GCAAGCAGAA GCTGCTGGCC TGCATAAAGT TTTTAAACTT 
CAAACTACTC TTACTTGCAA TTCCATGGTA CTCTTTGATC ATATGGGTTG TCTGAAGAAG 
TATGAAACTG TGCAAGACAT TTTGAAAGAA TTCTTTGATT TACGGTTAAG TTATTATGGT 
TTACGGAAGG AATGGCTTGT AGGAATGCTG GGAGCAGAAT CTACAAAGCT TAATAATCAA 
GCCCGTTTCA TTTTAGAGAA GATACAGGGG AAAATTACTA TAGAGAATAG GTCAAAGAAA 
GATTTGATTC AAATGTTAGT CCAGAGAGGT TATGAATCTG ACCCAGTGAA AGCGTGGAAA 
GAAGCACAAG AAAAGGCTGC AGAAGAGGAA GATTCACAAA ACCAACATGA TGACAGTTCC 
TCTGATTCAG GAACTCCTTC AGGCCCTGAT TTTAATTATA TTTTAAATAT GTCTCTATGG 
TCTCTTACTA AAGAAAAAGT TGAAGAACTC ATTAAACAGA GAGATACGAA AGGGCGAGAG 
GTTAATGATC TCAAGAGGAA GTCTCCTTCA GATCTTTGGA AAGAAGACTT AGCAGCATTT 
GTTGAAGAAT TGGATAAAGT GGAAGCACAA GAGCGGGAAG ATATTCTGGC AGGAATGTCT 
GGGAAAGCAA TAAAAGGTAA AGTCGGCAAA CCCAAAGTAA AGAAACTTCA GCTGGAGGAG 
ACAATGCCCT CGCCTTACGG GAGAAGAATT GTTCCAGAGA TTACTGCTAT GAAGGCAGAT 
GCCAGCAGAA AATTGCTGAA GAAGAAAAAG GGTGATCCTG ATACCACAGT AGTGAAAGTA 
GAATTTGATG AAGAATTTAG TGGAACACCA GCAGAAGGTA CAGGAGAGGA GACATTGACC 
CCATCTGCTC CTGTAAATAA AGGCCCCAAA CCCAAAAGAG AGAAGAAGGA GCCTGGTACC 
AGGGTTAGAA AAACACCTAC ATCAACTGGT AAAACTAATG CGAAGAAAGT GAAGAAACGG 
AATCCTTGGT CAGATGATGA GTCCAAATCA GAGAGTGACT TGGAAGAAGC AGAACCTGTG 
GTTATCCCAA GAGATTCTTT GCTTAGGAGA GCAGCTGCTG AAAGACCTAA GTACACTTTT 
GATTTCTCAG AAGAGGAGGA TGATGATGCT GCTGCTGCTG ATGACAGTAA TGATTTAGAA 
GAACTGAAAG TTAAAGCATC TCCCATAACA AATGATGGGG AAGATGAATT TGTTCCTTCA 
GATGGCTTAG ATAAAGATGA GTATGCTTTT TCTTCAGGCA AATCCAAAGC TACTCCAGAA 
AAATCTTCAA ATGACAAAAA AAGTCAGGAT TTTGGAAATC TTTTTTCATT TCCTTCATAT 
TCTCAGAAGT CAGAAGATGA TTCAGCTAAA TTTGACAGTA ATGAAGAAGA TACCGCTTCT 
GTTTTTGCTC CATCATTTGG TCTGAAGCAA ACAGATAAAC TTCCAAGTAA AACAGTTGCT 
GCTAAAAAGG GTAAACCACC TTCAGATACA GCCCCTAAAG CTAAGAGAGC CCCTAAACAG 
AAGAAGATAG TAGAGACTAT AAACTCTGAC TCAGACTCTG AATTTGGCAT TCCAAAGAAG 
ACTACAACAC CAAAAGGTAA AGGCCGAGGG GCAAAGAAAA GGAAAGCATC AGGCTCAGAA 
AATGAAGGTG ATTATAACCC TGGAAGGAAA CCATCTAAAA CAGCCAGCAA GAAACCGAAG 
AAAACATCTT TTGATCAGGA TTCAGATGTA GACATCTTCC CCTCAGACTT CACTTCTGAA 
CCACCGGCTC TCCCACGGAC TGGTCGGGCT AGGAAAGAAG TAAAATATTT TGCAGAGTCT 
GATGAAGAAG AAGATGTTGA TTTTGCAATG TTTAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CFP1 Regulates Histone H3K4 Trimethylation and Developmental Potential in Mouse Oocytes.
Cell reports20(5)1161-1172(2017 Aug)
Yu C,Fan X,Sha QQ,Wang HH,Li BT,Dai XX,Shen L,Liu J,Wang L,Liu K,Tang F,Fan HY

Genome Organization Drives Chromosome Fragility.
Cell170(3)507-521.e18(2017 Jul)
Canela A,Maman Y,Jung S,Wong N,Callen E,Day A,Kieffer-Kwon KR,Pekowska A,Zhang H,Rao SSP,Huang SC,Mckinnon PJ,Aplan PD,Pommier Y,Aiden EL,Casellas R,Nussenzweig A

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Top2b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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