NCU06338 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU06338
Entrez Gene ID	3878536
Full Name	DNA topoisomerase 2
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395868.1	XP_011394170.1	DNA topoisomerase 2
XM_011395869.1	XP_011394171.1	DNA topoisomerase 2, variant

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU06338 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU06338 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12099	XM_011395868.1 Latest version!	Neurospora crassa OR74A DNA topoisomerase 2 (NCU06338), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ONf12100	XM_011395869.1 Latest version!	Neurospora crassa OR74A DNA topoisomerase 2, variant (NCU06338), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU06338 ( XM_011395868.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394170.1 Neurospora crassa OR74A DNA topoisomerase 2 (NCU06338), mRNA.

Price & Availability↑

CloneID	ONf12099
Clone ID Related Accession (Same CDS sequence)	XM_011395868.1
Accession Version	XM_011395868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA topoisomerase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGTGCACGC CTCTCCTGTT GTCGTCCTGG GACCAACCGT TGTTGCTGCG TCTTGGAGGC 
CGCAGACTCG CACAGCCGCT GTGCACTCTC GCCCTTAAAC ACTTTTCTGC AGCAACCACA 
CCACCAACAA CAACCACAAC AACATCACTA CTACTTCGGT CATCACGCCC AGTACCGCCA 
AGATGGACTC CGACGTTGAG TCCATGTTCG AGGGGGAGTC CGACGCCTAT TCACCAGAGG 
TGGTACGTCA TGATGTCTTC TGCTTATCAT GCTAAGCCCA AGGCCAAGAA ACCCGCCGCC 
AAAAAGGCTG CCGCACCAAA GGCGCCCGCC GCAAAGAAGT TGACCCAGAC CACTCTCACC 
GGCGCAAAGG CCGCTGGCAA GAAGCGACCA AAACCCGACA GCGAGGATGA GGACGGATCC 
TTTGGTAGCG ACAAGGACGA CGGCATGCTC GACAACACCC CTCCCAATGC CAAAAAGCAG 
AAGAAGGCGC CGGTCAAGAA AGCCGCCGGC AAGCCTCTGG CCGAAATCGA GAACGATAGT 
ATGCAGATCG ATAGTGTCGC TCCCGCCAAA AAGACGGGCG CTGCAAAGAC GGCCACCGAA 
ATGTACCAAA AACTTACTCA GCTCGAACAT ATCCTCAAGC GTCCCGACAC CTACATTGGC 
TCCGTCGAGC GCTCCGAGCA AAAGATGTGG GTTTTCAACA AGGCTACCGG TCTCATGGAA 
AACCGCCAAA TCTCCTTCGT TCCCGGTCTC TACAAGATTT TCGACGAGAT TCTCGTCAAC 
GCCGCCGATA ACAGCCAGAG AGATAGCTCC ATGACCTTCC TCAAAGTGAC AATAAATCGC 
GAGTCTGGCG AAATCTCGGT CGAGAATAAC GGCAAGGGCA TTCCCGTAGA GATTCACGGC 
ACCGAAAAAT GCTACGTCCC CGAGCTCATC TTCGGTCATC TCCTGGCTGG TTCCAACTTC 
GATGACAACG AAAAGAAGAC GGTCGGCGGT CGCAACGGTT ACGGTGCCAA GCTCACAAAC 
ATCTTCAGCC GAGAATTCAC GCTTGAGTGC CAGGATTCCG TCAACGGCAA GAGATACAAG 
CAGACATGGA CCGACAACAT GAGCAAGATG TCACCACCCA AAATCACCTC CAACAAGGCT 
GCTGATTTTG TACGCATCAC TTTCCGCCCC GACTTCAGCA AGTTCGGCAT GGCTGACGGG 
ATCGACGATG ACCTGGAAGC CTTGCTCTAC CGACGTGTTT ACGACATGGC AGGTACTGTT 
AGTGGTGTCA AGGTTTGGCT TAATGGTGAA CACCTCAAGT TGAACTTCAA GACATACTGC 
GGGCTGTACG CCAAGGCCAT TGCCAAGGAG CGTGACGAGG TAGCTGACGG CGAAGATGTC 
ACTCCTGCCA CAGTCATCTT TGAGCAGCAA AGGTCCGAGG GTAAACTATG GGAAGTGGGC 
TTTACGGTTT CGGATGGCTC ATTCCAGCAA GTCTCGTTCG TCAACAATAT TGCCACCACC 
TCTGGCGGCT CCCACGTCAA TTACATTGCC GATCAGATCA TCGAAGTCCT CATGAAGGAG 
CTGCAAAAGA AGAAGGGCAA GGGACACGGT CTCAAGACGG CAAACGTCAA GAACCAGTTC 
TTCATCTTCA TCAACTGCCT GATTGACAAC CCAGCCTTCA GCTCGCAGAC AAAGGAGCAG 
TTGACGACCA AGCCCGCCAA ATTCGGCAGC AAGTGCCAGC TTGGTGACCT GTTCCTCAAG 
AAAGTGCGAC AATCGGAGGC CATTGACAAC TTGTTGAGCT TTGCCGACAA GAAGCGAGAC 
AAGGATTTGG CTAAGAACGA TGGCAGCAAG CGAAAGCGTA TCAGCAACGA CAAGCTGATC 
GAGGCCAATT ATGCTGGTGG AAGATATTCG CAAGAGTGTA CCCTTATTCT CACCGAAGGT 
GATTCCGCCA GAGGTCTTGC TGTCGCTGGT CGTGCTATCT TGGATCCTAA CCGTATCGGT 
GTCTTTCCCT TGCGTGGTAA GATGCTGAAC GTCCGTGACG CCAGCGCTGA TCAAATCTTG 
AAGAACAAGG AAGTCGAAAA CATCAAGAAG TTCCTTGGCC TCAAACACGG AAAGGTCTAC 
ACCGATACGA AGGATTTGCG ATACGGCCAT CTGATGATCA TGGCTGATCA GGATCTTGAC 
GGTAGTCATA TCAAGGGTCT TCTCATCAAC TTTTTCGAGT GCCAGTTTCC ATCACTCCTG 
CGCATTCCTA ATTTCTTCCA GGAGTTCATC ACGCCCGTTG TCAAGGTGTG GCAAGGTCCT 
AACCCGAAGA AGCCTCAAAG GCTACAGGGT TTCTTCACTC TGCCTCAGTA CGAGGAGTGG 
AAGGAAGCAC ACAGGAACGA ATTGCGCAGA TGGAAGTACA AGTATCTCAA GGGTTTGGGT 
AGTTCGACAA CAGACGACGC CCAGCTCTAC TTCACCAACT TGAACAAGCA TCTCAAAGAG 
TTCGATGTCA TGACTAGAGA GGAATCCGAG ATGTTCGAGT TGGCCTTCTC CAAGAAGAAG 
GCCGACGCTC GTAAAGAGTG GCTTGCCAAG TGCGAGCCGG GCACCTACCT CGACCATTCG 
ACCGAGAAGA TCTCCTACAC TGATTTCGTT AATCGCGAAC TCATTCTCTT CAGTATGGCA 
GACAACATTC GATCGATCCC GTCCATGGTG GATGGTCTGA AGCCCGGTCA ACGCAAGGTT 
CTGTTCGGCT GTTTCAAGCA AAACCTCATT CACGATCAAA AGGTTGTCGA GTTGGCCGGT 
TATGTGTCCA AGGAGGCAGC CTACCACCAC GGTGAACAGT CTCTGCAGCA AACGATTATT 
GGTCTGGCGC AGAACTTTGT CGGTTCAAAC AACATCAACT GTCTCGAGCC TAGCGGTAAC 
TTCGGTTCGC GTCTTTCCGG TGGTTCCGAT GCTGCCAGCG CTCGTTACAT CCACACGAGA 
CTGTCTCCCT TGGCGCGAAA GATCTTTCAC CCTCTCGATG AGCCCAATCT TGAATTCCAG 
TTCGACGATG GCAAACTGAT TGAGCCCAAG GTCTATGCTC CCATTATTCC CATGGTTTTG 
GTTAACGGTG CAGATGGTAT CGGTACCGGT TGGAGCACTT CCATTCCCAA CTACCACCCT 
CTTGACATTG TCGAGAACTT GAAGCGTCGC ATGGGCAGGG ACTCATCTGG TGACGGGGAG 
GAGAAGCCCT TCGAACCCAT GACTCCGTGG TTCCGTGGTT GGAAGGGCAC TCCTGAGCCA 
GACGGACCCA ACAGATTCAA GTTCAACGGT ATCGTGGAGG TCAACCCTCA GAACCCCAAT 
GAGGTTGATG TTACGGAGTT GCCCATTCGC ATGTGGACCG ATGATTTCAA GTCCAAGCTG 
GAAGAGATTA TCCGTGGTGA TAAGAGTCCT TCATGGATCA AGGACTACAA AGAGTACAAC 
GACCACCAAA CTGTCCACTT CATCATCGCC CTTGAGGAGA AGCACATGTC CTCGGCTCTT 
CGGGACGGTC TCATTGAAAA GTTCAAGCTT ACCAAGACAG TGGCCACAAC AAATCTAGTG 
GCCTTCGACA CCCGTGGTAA AATCCACAAG TATGAGAACC CGCAAGAAAT CATGGAGGAA 
TACTACCACT ACCGACTCAA CCTGTACGGT GAGCGCAAGA AGCACTGGCT CAAGGTTTAC 
CATGCAGACT ACCGCAAGCT GCAGAATCAA TACCGATTTA TTTCGGAAAT CATTGAGAAC 
AAGCTCGTCG TTTCCAAAAA GAAGAAGTCG GTCCTTGTCC AGGAACTTCG TGAACGCAAG 
TACGAGGCCT TCCCTCCGAA ATCAGATGGA AGGAATGTGA AGTCTCCTGA TGAGGAGCTC 
GGTGCTGCGG ACAACGAGGA CGAGGAGGAT GCCGTTGGCG GAGCCCGCGA TTATGACTAT 
CTCCTTTCGA TGCCCATTTG GTCGTTGACC TTTGAGCGCC TCGACAAGCT CAAGCAGCAG 
ATTGCTGCAA AGAAGGCTGA GCACGATGAA CTCGATGCAC TCAGCGAAAA GGATCTGTGG 
TGCAGAGATC TCGATGCTTT CACCGCTGAG TGGGAAGAGC AGTTGAGGCT GGAAGATGAA 
GTCCGGAAAG AGATCAGGCA GAAGGGACGC CGTGTCTCGT ACAAGGGCGG TGCTGTCTCC 
AGACTCAAGA AGTCCAGAGG TGCCAAGGAT GACGAGGACT TCGACCCGGG TGCCAAGAAG 
GCCAAAGCCA AGGCAGCTCC CAAGGCTGAG ACAATCAAGA CACAGCAGCG TTTTCTAGAG 
AAGTTCTCTG GGATGGCGAA ACCCAAGCCT AAGTCGACCG CTTTCACCGA CGGAGCTGAT 
GACCTCTCAG ACGATGACTT CGACTTGTTG AACAAGAAGG CCCCGGCTGC AGCCAAAAAG 
AAGGATGAGG ATGCGGAGCC CGTTACTACC ATCGCCAGTG TGCGCGCCAA GCGTGCCACC 
GTAGCGAAGC CTAAGACTTA TGCGCTCAGT GATGAAGATT CGGACGATGA GTTCTTGGAC 
ATCGCAAAGG TTACCACAAC TTCTGAGAAA ACGACCACGT CGGCTTCAGC TTCGGTTTCA 
GCGGCAGAAC AATCTGAGCA ACCTGTAGCA AAGCCCGCGA GGCGTGCTGC AGGAGCCAAG 
AAGCCTATCG TTGATGAGGA TTCTGAATCC GATTTGGATG ACTTCATTGA CGACGAGTCC 
GAGGAAGAGG TGATTCCTAA GCCCACCACC AAGCGAGCCA CCGCTGCCAA GAAGCCGGCC 
CTCGAAGATG AAGACTCCGA TGTCGAGTTC CAGGACGCTC CTGAAGAGCC TATCCCGCCC 
ACCCCCAAGC CGGAGGCCAA GAAGGCTACG GCCAAGAAGC CAATTGTAGT TGAAGACTCT 
GACGAAGACA TGATGCTCAG CGAAGCAGAG AAGCCTGCGC ACGCAGCTAA GCCCCCCACT 
AAGCGCGCTG CTGCTGTCAA GAAATCACCT GTTCTCGAAG ATGATGACTT CGAGAGCGAC 
GCTGATGACA AGATTTTGAG CGAGGCAGAA AAACCTGCGC CGGCTAAGGC CACCGCCAAG 
CGTGCGGCTG CTGCCAAGAA GTCACCTGTT CTTGAGGACG ATGATCTTGA GAGCGACGGT 
GATGACAAGA TGGTAAGCGA GGCTGAGAAA CCCGCTCCGG CCAATCCCAG GAAGCGCGCG 
GCGGCCGCTA AGGCCAAGTC CTTCTTCATA GACGAAGAAG ACTCCGAAAG CGAAGCTTCT 
GATGACGACG ACATGCTTCT TGACGTCGGC TCCCTAGTCA AGGGTATAGG AGCCCCTGCC 
GCGACGAGCG AGGGCCCGAG GCTCAGCCTC TTTTCCATGT CTCGGCCAGA AGGCGAGGGT 
AGCGGTATCG GCCTGCCCAA AGTGAAGAGC AGGGCTTTCC ACACCAAAGC TTTTCTCGAC 
AACGATAGCC AGGATGACAC CAACTACGAG GCTCTTGCCA AGTCGTCCCC GAGGAAGCAT 
TCTAAGACCG GTGAGGCCGA CGAGGGTTCG GAGGACGATT CCATCGGCAA GAAGGTCAAC 
GCTGCCCCTG TAGTGGCCAA GAAGCGCGGT CGTCCTGCCG GCAGTAAAAA CAAGGCCACC 
GACGACGACG CTGCTGCCCC GAAGCCCAAG CCCAAGGTCA CCGCCAAGAA GGCTGCTGCT 
GCTGCTGCTG GAGAGACGGC CTCTGCCGTT CCGGCCAAGC TCAAGACGCT GGTCCATCTA 
TCGCCTGCTG CTAAGGCTTA TGCCAAGAAG AACCAGAAGG CAACGAAGCT CGCTAGTGAC 
GACGAGGATG ACCTTGATGG CGATGACGTC GTAATGAAGG ATCCTGCGCC TGCTCCGGCT 
CGCAGCAGGC CTGGTCGCGC GGCCGCTGCT AGGAAGCCGA TTGTCATTGA TAGCGCAAGT 
GAGGATGATG ATGACTCGTT TGGGGATATT GGCGTGAGCA GCAAGGCAAA GAAGGGTGGT 
AGGAAGGTGG CTGATGATGC TTTTGATATG GAAGATAGTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394170.1
CDS	323..6307
Translation	MCTPLLLSSWDQPLLLRLGGRRLAQPLCTLALKHFSAATTPPTTTTTTSLLLRSSRPVPPRWTPTLSPCSRGSPTPIHQRWYVMMSSAYHAKPKAKKPAAKKAAAPKAPAAKKLTQTTLTGAKAAGKKRPKPDSEDEDGSFGSDKDDGMLDNTPPNAKKQKKAPVKKAAGKPLAEIENDSMQIDSVAPAKKTGAAKTATEMYQKLTQLEHILKRPDTYIGSVERSEQKMWVFNKATGLMENRQISFVPGLYKIFDEILVNAADNSQRDSSMTFLKVTINRESGEISVENNGKGIPVEIHGTEKCYVPELIFGHLLAGSNFDDNEKKTVGGRNGYGAKLTNIFSREFTLECQDSVNGKRYKQTWTDNMSKMSPPKITSNKAADFVRITFRPDFSKFGMADGIDDDLEALLYRRVYDMAGTVSGVKVWLNGEHLKLNFKTYCGLYAKAIAKERDEVADGEDVTPATVIFEQQRSEGKLWEVGFTVSDGSFQQVSFVNNIATTSGGSHVNYIADQIIEVLMKELQKKKGKGHGLKTANVKNQFFIFINCLIDNPAFSSQTKEQLTTKPAKFGSKCQLGDLFLKKVRQSEAIDNLLSFADKKRDKDLAKNDGSKRKRISNDKLIEANYAGGRYSQECTLILTEGDSARGLAVAGRAILDPNRIGVFPLRGKMLNVRDASADQILKNKEVENIKKFLGLKHGKVYTDTKDLRYGHLMIMADQDLDGSHIKGLLINFFECQFPSLLRIPNFFQEFITPVVKVWQGPNPKKPQRLQGFFTLPQYEEWKEAHRNELRRWKYKYLKGLGSSTTDDAQLYFTNLNKHLKEFDVMTREESEMFELAFSKKKADARKEWLAKCEPGTYLDHSTEKISYTDFVNRELILFSMADNIRSIPSMVDGLKPGQRKVLFGCFKQNLIHDQKVVELAGYVSKEAAYHHGEQSLQQTIIGLAQNFVGSNNINCLEPSGNFGSRLSGGSDAASARYIHTRLSPLARKIFHPLDEPNLEFQFDDGKLIEPKVYAPIIPMVLVNGADGIGTGWSTSIPNYHPLDIVENLKRRMGRDSSGDGEEKPFEPMTPWFRGWKGTPEPDGPNRFKFNGIVEVNPQNPNEVDVTELPIRMWTDDFKSKLEEIIRGDKSPSWIKDYKEYNDHQTVHFIIALEEKHMSSALRDGLIEKFKLTKTVATTNLVAFDTRGKIHKYENPQEIMEEYYHYRLNLYGERKKHWLKVYHADYRKLQNQYRFISEIIENKLVVSKKKKSVLVQELRERKYEAFPPKSDGRNVKSPDEELGAADNEDEEDAVGGARDYDYLLSMPIWSLTFERLDKLKQQIAAKKAEHDELDALSEKDLWCRDLDAFTAEWEEQLRLEDEVRKEIRQKGRRVSYKGGAVSRLKKSRGAKDDEDFDPGAKKAKAKAAPKAETIKTQQRFLEKFSGMAKPKPKSTAFTDGADDLSDDDFDLLNKKAPAAAKKKDEDAEPVTTIASVRAKRATVAKPKTYALSDEDSDDEFLDIAKVTTTSEKTTTSASASVSAAEQSEQPVAKPARRAAGAKKPIVDEDSESDLDDFIDDESEEEVIPKPTTKRATAAKKPALEDEDSDVEFQDAPEEPIPPTPKPEAKKATAKKPIVVEDSDEDMMLSEAEKPAHAAKPPTKRAAAVKKSPVLEDDDFESDADDKILSEAEKPAPAKATAKRAAAAKKSPVLEDDDLESDGDDKMVSEAEKPAPANPRKRAAAAKAKSFFIDEEDSESEASDDDDMLLDVGSLVKGIGAPAATSEGPRLSLFSMSRPEGEGSGIGLPKVKSRAFHTKAFLDNDSQDDTNYEALAKSSPRKHSKTGEADEGSEDDSIGKKVNAAPVVAKKRGRPAGSKNKATDDDAAAPKPKPKVTAKKAAAAAAGETASAVPAKLKTLVHLSPAAKAYAKKNQKATKLASDDEDDLDGDDVVMKDPAPAPARSRPGRAAAARKPIVIDSASEDDDDSFGDIGVSSKAKKGGRKVADDAFDMEDSE

Target ORF information:

RefSeq Version	XM_011395868.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA topoisomerase 2 (NCU06338), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395868.1

ORF Insert Sequence:

ATGTGCACGC CTCTCCTGTT GTCGTCCTGG GACCAACCGT TGTTGCTGCG TCTTGGAGGC 
CGCAGACTCG CACAGCCGCT GTGCACTCTC GCCCTTAAAC ACTTTTCTGC AGCAACCACA 
CCACCAACAA CAACCACAAC AACATCACTA CTACTTCGGT CATCACGCCC AGTACCGCCA 
AGATGGACTC CGACGTTGAG TCCATGTTCG AGGGGGAGTC CGACGCCTAT TCACCAGAGG 
TGGTACGTCA TGATGTCTTC TGCTTATCAT GCTAAGCCCA AGGCCAAGAA ACCCGCCGCC 
AAAAAGGCTG CCGCACCAAA GGCGCCCGCC GCAAAGAAGT TGACCCAGAC CACTCTCACC 
GGCGCAAAGG CCGCTGGCAA GAAGCGACCA AAACCCGACA GCGAGGATGA GGACGGATCC 
TTTGGTAGCG ACAAGGACGA CGGCATGCTC GACAACACCC CTCCCAATGC CAAAAAGCAG 
AAGAAGGCGC CGGTCAAGAA AGCCGCCGGC AAGCCTCTGG CCGAAATCGA GAACGATAGT 
ATGCAGATCG ATAGTGTCGC TCCCGCCAAA AAGACGGGCG CTGCAAAGAC GGCCACCGAA 
ATGTACCAAA AACTTACTCA GCTCGAACAT ATCCTCAAGC GTCCCGACAC CTACATTGGC 
TCCGTCGAGC GCTCCGAGCA AAAGATGTGG GTTTTCAACA AGGCTACCGG TCTCATGGAA 
AACCGCCAAA TCTCCTTCGT TCCCGGTCTC TACAAGATTT TCGACGAGAT TCTCGTCAAC 
GCCGCCGATA ACAGCCAGAG AGATAGCTCC ATGACCTTCC TCAAAGTGAC AATAAATCGC 
GAGTCTGGCG AAATCTCGGT CGAGAATAAC GGCAAGGGCA TTCCCGTAGA GATTCACGGC 
ACCGAAAAAT GCTACGTCCC CGAGCTCATC TTCGGTCATC TCCTGGCTGG TTCCAACTTC 
GATGACAACG AAAAGAAGAC GGTCGGCGGT CGCAACGGTT ACGGTGCCAA GCTCACAAAC 
ATCTTCAGCC GAGAATTCAC GCTTGAGTGC CAGGATTCCG TCAACGGCAA GAGATACAAG 
CAGACATGGA CCGACAACAT GAGCAAGATG TCACCACCCA AAATCACCTC CAACAAGGCT 
GCTGATTTTG TACGCATCAC TTTCCGCCCC GACTTCAGCA AGTTCGGCAT GGCTGACGGG 
ATCGACGATG ACCTGGAAGC CTTGCTCTAC CGACGTGTTT ACGACATGGC AGGTACTGTT 
AGTGGTGTCA AGGTTTGGCT TAATGGTGAA CACCTCAAGT TGAACTTCAA GACATACTGC 
GGGCTGTACG CCAAGGCCAT TGCCAAGGAG CGTGACGAGG TAGCTGACGG CGAAGATGTC 
ACTCCTGCCA CAGTCATCTT TGAGCAGCAA AGGTCCGAGG GTAAACTATG GGAAGTGGGC 
TTTACGGTTT CGGATGGCTC ATTCCAGCAA GTCTCGTTCG TCAACAATAT TGCCACCACC 
TCTGGCGGCT CCCACGTCAA TTACATTGCC GATCAGATCA TCGAAGTCCT CATGAAGGAG 
CTGCAAAAGA AGAAGGGCAA GGGACACGGT CTCAAGACGG CAAACGTCAA GAACCAGTTC 
TTCATCTTCA TCAACTGCCT GATTGACAAC CCAGCCTTCA GCTCGCAGAC AAAGGAGCAG 
TTGACGACCA AGCCCGCCAA ATTCGGCAGC AAGTGCCAGC TTGGTGACCT GTTCCTCAAG 
AAAGTGCGAC AATCGGAGGC CATTGACAAC TTGTTGAGCT TTGCCGACAA GAAGCGAGAC 
AAGGATTTGG CTAAGAACGA TGGCAGCAAG CGAAAGCGTA TCAGCAACGA CAAGCTGATC 
GAGGCCAATT ATGCTGGTGG AAGATATTCG CAAGAGTGTA CCCTTATTCT CACCGAAGGT 
GATTCCGCCA GAGGTCTTGC TGTCGCTGGT CGTGCTATCT TGGATCCTAA CCGTATCGGT 
GTCTTTCCCT TGCGTGGTAA GATGCTGAAC GTCCGTGACG CCAGCGCTGA TCAAATCTTG 
AAGAACAAGG AAGTCGAAAA CATCAAGAAG TTCCTTGGCC TCAAACACGG AAAGGTCTAC 
ACCGATACGA AGGATTTGCG ATACGGCCAT CTGATGATCA TGGCTGATCA GGATCTTGAC 
GGTAGTCATA TCAAGGGTCT TCTCATCAAC TTTTTCGAGT GCCAGTTTCC ATCACTCCTG 
CGCATTCCTA ATTTCTTCCA GGAGTTCATC ACGCCCGTTG TCAAGGTGTG GCAAGGTCCT 
AACCCGAAGA AGCCTCAAAG GCTACAGGGT TTCTTCACTC TGCCTCAGTA CGAGGAGTGG 
AAGGAAGCAC ACAGGAACGA ATTGCGCAGA TGGAAGTACA AGTATCTCAA GGGTTTGGGT 
AGTTCGACAA CAGACGACGC CCAGCTCTAC TTCACCAACT TGAACAAGCA TCTCAAAGAG 
TTCGATGTCA TGACTAGAGA GGAATCCGAG ATGTTCGAGT TGGCCTTCTC CAAGAAGAAG 
GCCGACGCTC GTAAAGAGTG GCTTGCCAAG TGCGAGCCGG GCACCTACCT CGACCATTCG 
ACCGAGAAGA TCTCCTACAC TGATTTCGTT AATCGCGAAC TCATTCTCTT CAGTATGGCA 
GACAACATTC GATCGATCCC GTCCATGGTG GATGGTCTGA AGCCCGGTCA ACGCAAGGTT 
CTGTTCGGCT GTTTCAAGCA AAACCTCATT CACGATCAAA AGGTTGTCGA GTTGGCCGGT 
TATGTGTCCA AGGAGGCAGC CTACCACCAC GGTGAACAGT CTCTGCAGCA AACGATTATT 
GGTCTGGCGC AGAACTTTGT CGGTTCAAAC AACATCAACT GTCTCGAGCC TAGCGGTAAC 
TTCGGTTCGC GTCTTTCCGG TGGTTCCGAT GCTGCCAGCG CTCGTTACAT CCACACGAGA 
CTGTCTCCCT TGGCGCGAAA GATCTTTCAC CCTCTCGATG AGCCCAATCT TGAATTCCAG 
TTCGACGATG GCAAACTGAT TGAGCCCAAG GTCTATGCTC CCATTATTCC CATGGTTTTG 
GTTAACGGTG CAGATGGTAT CGGTACCGGT TGGAGCACTT CCATTCCCAA CTACCACCCT 
CTTGACATTG TCGAGAACTT GAAGCGTCGC ATGGGCAGGG ACTCATCTGG TGACGGGGAG 
GAGAAGCCCT TCGAACCCAT GACTCCGTGG TTCCGTGGTT GGAAGGGCAC TCCTGAGCCA 
GACGGACCCA ACAGATTCAA GTTCAACGGT ATCGTGGAGG TCAACCCTCA GAACCCCAAT 
GAGGTTGATG TTACGGAGTT GCCCATTCGC ATGTGGACCG ATGATTTCAA GTCCAAGCTG 
GAAGAGATTA TCCGTGGTGA TAAGAGTCCT TCATGGATCA AGGACTACAA AGAGTACAAC 
GACCACCAAA CTGTCCACTT CATCATCGCC CTTGAGGAGA AGCACATGTC CTCGGCTCTT 
CGGGACGGTC TCATTGAAAA GTTCAAGCTT ACCAAGACAG TGGCCACAAC AAATCTAGTG 
GCCTTCGACA CCCGTGGTAA AATCCACAAG TATGAGAACC CGCAAGAAAT CATGGAGGAA 
TACTACCACT ACCGACTCAA CCTGTACGGT GAGCGCAAGA AGCACTGGCT CAAGGTTTAC 
CATGCAGACT ACCGCAAGCT GCAGAATCAA TACCGATTTA TTTCGGAAAT CATTGAGAAC 
AAGCTCGTCG TTTCCAAAAA GAAGAAGTCG GTCCTTGTCC AGGAACTTCG TGAACGCAAG 
TACGAGGCCT TCCCTCCGAA ATCAGATGGA AGGAATGTGA AGTCTCCTGA TGAGGAGCTC 
GGTGCTGCGG ACAACGAGGA CGAGGAGGAT GCCGTTGGCG GAGCCCGCGA TTATGACTAT 
CTCCTTTCGA TGCCCATTTG GTCGTTGACC TTTGAGCGCC TCGACAAGCT CAAGCAGCAG 
ATTGCTGCAA AGAAGGCTGA GCACGATGAA CTCGATGCAC TCAGCGAAAA GGATCTGTGG 
TGCAGAGATC TCGATGCTTT CACCGCTGAG TGGGAAGAGC AGTTGAGGCT GGAAGATGAA 
GTCCGGAAAG AGATCAGGCA GAAGGGACGC CGTGTCTCGT ACAAGGGCGG TGCTGTCTCC 
AGACTCAAGA AGTCCAGAGG TGCCAAGGAT GACGAGGACT TCGACCCGGG TGCCAAGAAG 
GCCAAAGCCA AGGCAGCTCC CAAGGCTGAG ACAATCAAGA CACAGCAGCG TTTTCTAGAG 
AAGTTCTCTG GGATGGCGAA ACCCAAGCCT AAGTCGACCG CTTTCACCGA CGGAGCTGAT 
GACCTCTCAG ACGATGACTT CGACTTGTTG AACAAGAAGG CCCCGGCTGC AGCCAAAAAG 
AAGGATGAGG ATGCGGAGCC CGTTACTACC ATCGCCAGTG TGCGCGCCAA GCGTGCCACC 
GTAGCGAAGC CTAAGACTTA TGCGCTCAGT GATGAAGATT CGGACGATGA GTTCTTGGAC 
ATCGCAAAGG TTACCACAAC TTCTGAGAAA ACGACCACGT CGGCTTCAGC TTCGGTTTCA 
GCGGCAGAAC AATCTGAGCA ACCTGTAGCA AAGCCCGCGA GGCGTGCTGC AGGAGCCAAG 
AAGCCTATCG TTGATGAGGA TTCTGAATCC GATTTGGATG ACTTCATTGA CGACGAGTCC 
GAGGAAGAGG TGATTCCTAA GCCCACCACC AAGCGAGCCA CCGCTGCCAA GAAGCCGGCC 
CTCGAAGATG AAGACTCCGA TGTCGAGTTC CAGGACGCTC CTGAAGAGCC TATCCCGCCC 
ACCCCCAAGC CGGAGGCCAA GAAGGCTACG GCCAAGAAGC CAATTGTAGT TGAAGACTCT 
GACGAAGACA TGATGCTCAG CGAAGCAGAG AAGCCTGCGC ACGCAGCTAA GCCCCCCACT 
AAGCGCGCTG CTGCTGTCAA GAAATCACCT GTTCTCGAAG ATGATGACTT CGAGAGCGAC 
GCTGATGACA AGATTTTGAG CGAGGCAGAA AAACCTGCGC CGGCTAAGGC CACCGCCAAG 
CGTGCGGCTG CTGCCAAGAA GTCACCTGTT CTTGAGGACG ATGATCTTGA GAGCGACGGT 
GATGACAAGA TGGTAAGCGA GGCTGAGAAA CCCGCTCCGG CCAATCCCAG GAAGCGCGCG 
GCGGCCGCTA AGGCCAAGTC CTTCTTCATA GACGAAGAAG ACTCCGAAAG CGAAGCTTCT 
GATGACGACG ACATGCTTCT TGACGTCGGC TCCCTAGTCA AGGGTATAGG AGCCCCTGCC 
GCGACGAGCG AGGGCCCGAG GCTCAGCCTC TTTTCCATGT CTCGGCCAGA AGGCGAGGGT 
AGCGGTATCG GCCTGCCCAA AGTGAAGAGC AGGGCTTTCC ACACCAAAGC TTTTCTCGAC 
AACGATAGCC AGGATGACAC CAACTACGAG GCTCTTGCCA AGTCGTCCCC GAGGAAGCAT 
TCTAAGACCG GTGAGGCCGA CGAGGGTTCG GAGGACGATT CCATCGGCAA GAAGGTCAAC 
GCTGCCCCTG TAGTGGCCAA GAAGCGCGGT CGTCCTGCCG GCAGTAAAAA CAAGGCCACC 
GACGACGACG CTGCTGCCCC GAAGCCCAAG CCCAAGGTCA CCGCCAAGAA GGCTGCTGCT 
GCTGCTGCTG GAGAGACGGC CTCTGCCGTT CCGGCCAAGC TCAAGACGCT GGTCCATCTA 
TCGCCTGCTG CTAAGGCTTA TGCCAAGAAG AACCAGAAGG CAACGAAGCT CGCTAGTGAC 
GACGAGGATG ACCTTGATGG CGATGACGTC GTAATGAAGG ATCCTGCGCC TGCTCCGGCT 
CGCAGCAGGC CTGGTCGCGC GGCCGCTGCT AGGAAGCCGA TTGTCATTGA TAGCGCAAGT 
GAGGATGATG ATGACTCGTT TGGGGATATT GGCGTGAGCA GCAAGGCAAA GAAGGGTGGT 
AGGAAGGTGG CTGATGATGC TTTTGATATG GAAGATAGTG AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU06338 ( XM_011395869.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394171.1 Neurospora crassa OR74A DNA topoisomerase 2, variant (NCU06338), mRNA.

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CloneID	ONf12100
Clone ID Related Accession (Same CDS sequence)	XM_011395869.1
Accession Version	XM_011395869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	DNA topoisomerase 2, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504).

ATGGACTCCG ACGTTGAGTC CATGTTCGAG GGGGAGTCCG ACGCCTATTC ACCAGAGGTG 
AAGCCCAAGG CCAAGAAACC CGCCGCCAAA AAGGCTGCCG CACCAAAGGC GCCCGCCGCA 
AAGAAGTTGA CCCAGACCAC TCTCACCGGC GCAAAGGCCG CTGGCAAGAA GCGACCAAAA 
CCCGACAGCG AGGATGAGGA CGGATCCTTT GGTAGCGACA AGGACGACGG CATGCTCGAC 
AACACCCCTC CCAATGCCAA AAAGCAGAAG AAGGCGCCGG TCAAGAAAGC CGCCGGCAAG 
CCTCTGGCCG AAATCGAGAA CGATAGTATG CAGATCGATA GTGTCGCTCC CGCCAAAAAG 
ACGGGCGCTG CAAAGACGGC CACCGAAATG TACCAAAAAC TTACTCAGCT CGAACATATC 
CTCAAGCGTC CCGACACCTA CATTGGCTCC GTCGAGCGCT CCGAGCAAAA GATGTGGGTT 
TTCAACAAGG CTACCGGTCT CATGGAAAAC CGCCAAATCT CCTTCGTTCC CGGTCTCTAC 
AAGATTTTCG ACGAGATTCT CGTCAACGCC GCCGATAACA GCCAGAGAGA TAGCTCCATG 
ACCTTCCTCA AAGTGACAAT AAATCGCGAG TCTGGCGAAA TCTCGGTCGA GAATAACGGC 
AAGGGCATTC CCGTAGAGAT TCACGGCACC GAAAAATGCT ACGTCCCCGA GCTCATCTTC 
GGTCATCTCC TGGCTGGTTC CAACTTCGAT GACAACGAAA AGAAGACGGT CGGCGGTCGC 
AACGGTTACG GTGCCAAGCT CACAAACATC TTCAGCCGAG AATTCACGCT TGAGTGCCAG 
GATTCCGTCA ACGGCAAGAG ATACAAGCAG ACATGGACCG ACAACATGAG CAAGATGTCA 
CCACCCAAAA TCACCTCCAA CAAGGCTGCT GATTTTGTAC GCATCACTTT CCGCCCCGAC 
TTCAGCAAGT TCGGCATGGC TGACGGGATC GACGATGACC TGGAAGCCTT GCTCTACCGA 
CGTGTTTACG ACATGGCAGG TACTGTTAGT GGTGTCAAGG TTTGGCTTAA TGGTGAACAC 
CTCAAGTTGA ACTTCAAGAC ATACTGCGGG CTGTACGCCA AGGCCATTGC CAAGGAGCGT 
GACGAGGTAG CTGACGGCGA AGATGTCACT CCTGCCACAG TCATCTTTGA GCAGCAAAGG 
TCCGAGGGTA AACTATGGGA AGTGGGCTTT ACGGTTTCGG ATGGCTCATT CCAGCAAGTC 
TCGTTCGTCA ACAATATTGC CACCACCTCT GGCGGCTCCC ACGTCAATTA CATTGCCGAT 
CAGATCATCG AAGTCCTCAT GAAGGAGCTG CAAAAGAAGA AGGGCAAGGG ACACGGTCTC 
AAGACGGCAA ACGTCAAGAA CCAGTTCTTC ATCTTCATCA ACTGCCTGAT TGACAACCCA 
GCCTTCAGCT CGCAGACAAA GGAGCAGTTG ACGACCAAGC CCGCCAAATT CGGCAGCAAG 
TGCCAGCTTG GTGACCTGTT CCTCAAGAAA GTGCGACAAT CGGAGGCCAT TGACAACTTG 
TTGAGCTTTG CCGACAAGAA GCGAGACAAG GATTTGGCTA AGAACGATGG CAGCAAGCGA 
AAGCGTATCA GCAACGACAA GCTGATCGAG GCCAATTATG CTGGTGGAAG ATATTCGCAA 
GAGTGTACCC TTATTCTCAC CGAAGGTGAT TCCGCCAGAG GTCTTGCTGT CGCTGGTCGT 
GCTATCTTGG ATCCTAACCG TATCGGTGTC TTTCCCTTGC GTGGTAAGAT GCTGAACGTC 
CGTGACGCCA GCGCTGATCA AATCTTGAAG AACAAGGAAG TCGAAAACAT CAAGAAGTTC 
CTTGGCCTCA AACACGGAAA GGTCTACACC GATACGAAGG ATTTGCGATA CGGCCATCTG 
ATGATCATGG CTGATCAGGA TCTTGACGGT AGTCATATCA AGGGTCTTCT CATCAACTTT 
TTCGAGTGCC AGTTTCCATC ACTCCTGCGC ATTCCTAATT TCTTCCAGGA GTTCATCACG 
CCCGTTGTCA AGGTGTGGCA AGGTCCTAAC CCGAAGAAGC CTCAAAGGCT ACAGGGTTTC 
TTCACTCTGC CTCAGTACGA GGAGTGGAAG GAAGCACACA GGAACGAATT GCGCAGATGG 
AAGTACAAGT ATCTCAAGGG TTTGGGTAGT TCGACAACAG ACGACGCCCA GCTCTACTTC 
ACCAACTTGA ACAAGCATCT CAAAGAGTTC GATGTCATGA CTAGAGAGGA ATCCGAGATG 
TTCGAGTTGG CCTTCTCCAA GAAGAAGGCC GACGCTCGTA AAGAGTGGCT TGCCAAGTGC 
GAGCCGGGCA CCTACCTCGA CCATTCGACC GAGAAGATCT CCTACACTGA TTTCGTTAAT 
CGCGAACTCA TTCTCTTCAG TATGGCAGAC AACATTCGAT CGATCCCGTC CATGGTGGAT 
GGTCTGAAGC CCGGTCAACG CAAGGTTCTG TTCGGCTGTT TCAAGCAAAA CCTCATTCAC 
GATCAAAAGG TTGTCGAGTT GGCCGGTTAT GTGTCCAAGG AGGCAGCCTA CCACCACGGT 
GAACAGTCTC TGCAGCAAAC GATTATTGGT CTGGCGCAGA ACTTTGTCGG TTCAAACAAC 
ATCAACTGTC TCGAGCCTAG CGGTAACTTC GGTTCGCGTC TTTCCGGTGG TTCCGATGCT 
GCCAGCGCTC GTTACATCCA CACGAGACTG TCTCCCTTGG CGCGAAAGAT CTTTCACCCT 
CTCGATGAGC CCAATCTTGA ATTCCAGTTC GACGATGGCA AACTGATTGA GCCCAAGGTC 
TATGCTCCCA TTATTCCCAT GGTTTTGGTT AACGGTGCAG ATGGTATCGG TACCGGTTGG 
AGCACTTCCA TTCCCAACTA CCACCCTCTT GACATTGTCG AGAACTTGAA GCGTCGCATG 
GGCAGGGACT CATCTGGTGA CGGGGAGGAG AAGCCCTTCG AACCCATGAC TCCGTGGTTC 
CGTGGTTGGA AGGGCACTCC TGAGCCAGAC GGACCCAACA GATTCAAGTT CAACGGTATC 
GTGGAGGTCA ACCCTCAGAA CCCCAATGAG GTTGATGTTA CGGAGTTGCC CATTCGCATG 
TGGACCGATG ATTTCAAGTC CAAGCTGGAA GAGATTATCC GTGGTGATAA GAGTCCTTCA 
TGGATCAAGG ACTACAAAGA GTACAACGAC CACCAAACTG TCCACTTCAT CATCGCCCTT 
GAGGAGAAGC ACATGTCCTC GGCTCTTCGG GACGGTCTCA TTGAAAAGTT CAAGCTTACC 
AAGACAGTGG CCACAACAAA TCTAGTGGCC TTCGACACCC GTGGTAAAAT CCACAAGTAT 
GAGAACCCGC AAGAAATCAT GGAGGAATAC TACCACTACC GACTCAACCT GTACGGTGAG 
CGCAAGAAGC ACTGGCTCAA GGTTTACCAT GCAGACTACC GCAAGCTGCA GAATCAATAC 
CGATTTATTT CGGAAATCAT TGAGAACAAG CTCGTCGTTT CCAAAAAGAA GAAGTCGGTC 
CTTGTCCAGG AACTTCGTGA ACGCAAGTAC GAGGCCTTCC CTCCGAAATC AGATGGAAGG 
AATGTGAAGT CTCCTGATGA GGAGCTCGGT GCTGCGGACA ACGAGGACGA GGAGGATGCC 
GTTGGCGGAG CCCGCGATTA TGACTATCTC CTTTCGATGC CCATTTGGTC GTTGACCTTT 
GAGCGCCTCG ACAAGCTCAA GCAGCAGATT GCTGCAAAGA AGGCTGAGCA CGATGAACTC 
GATGCACTCA GCGAAAAGGA TCTGTGGTGC AGAGATCTCG ATGCTTTCAC CGCTGAGTGG 
GAAGAGCAGT TGAGGCTGGA AGATGAAGTC CGGAAAGAGA TCAGGCAGAA GGGACGCCGT 
GTCTCGTACA AGGGCGGTGC TGTCTCCAGA CTCAAGAAGT CCAGAGGTGC CAAGGATGAC 
GAGGACTTCG ACCCGGGTGC CAAGAAGGCC AAAGCCAAGG CAGCTCCCAA GGCTGAGACA 
ATCAAGACAC AGCAGCGTTT TCTAGAGAAG TTCTCTGGGA TGGCGAAACC CAAGCCTAAG 
TCGACCGCTT TCACCGACGG AGCTGATGAC CTCTCAGACG ATGACTTCGA CTTGTTGAAC 
AAGAAGGCCC CGGCTGCAGC CAAAAAGAAG GATGAGGATG CGGAGCCCGT TACTACCATC 
GCCAGTGTGC GCGCCAAGCG TGCCACCGTA GCGAAGCCTA AGACTTATGC GCTCAGTGAT 
GAAGATTCGG ACGATGAGTT CTTGGACATC GCAAAGGTTA CCACAACTTC TGAGAAAACG 
ACCACGTCGG CTTCAGCTTC GGTTTCAGCG GCAGAACAAT CTGAGCAACC TGTAGCAAAG 
CCCGCGAGGC GTGCTGCAGG AGCCAAGAAG CCTATCGTTG ATGAGGATTC TGAATCCGAT 
TTGGATGACT TCATTGACGA CGAGTCCGAG GAAGAGGTGA TTCCTAAGCC CACCACCAAG 
CGAGCCACCG CTGCCAAGAA GCCGGCCCTC GAAGATGAAG ACTCCGATGT CGAGTTCCAG 
GACGCTCCTG AAGAGCCTAT CCCGCCCACC CCCAAGCCGG AGGCCAAGAA GGCTACGGCC 
AAGAAGCCAA TTGTAGTTGA AGACTCTGAC GAAGACATGA TGCTCAGCGA AGCAGAGAAG 
CCTGCGCACG CAGCTAAGCC CCCCACTAAG CGCGCTGCTG CTGTCAAGAA ATCACCTGTT 
CTCGAAGATG ATGACTTCGA GAGCGACGCT GATGACAAGA TTTTGAGCGA GGCAGAAAAA 
CCTGCGCCGG CTAAGGCCAC CGCCAAGCGT GCGGCTGCTG CCAAGAAGTC ACCTGTTCTT 
GAGGACGATG ATCTTGAGAG CGACGGTGAT GACAAGATGG TAAGCGAGGC TGAGAAACCC 
GCTCCGGCCA ATCCCAGGAA GCGCGCGGCG GCCGCTAAGG CCAAGTCCTT CTTCATAGAC 
GAAGAAGACT CCGAAAGCGA AGCTTCTGAT GACGACGACA TGCTTCTTGA CGTCGGCTCC 
CTAGTCAAGG GTATAGGAGC CCCTGCCGCG ACGAGCGAGG GCCCGAGGCT CAGCCTCTTT 
TCCATGTCTC GGCCAGAAGG CGAGGGTAGC GGTATCGGCC TGCCCAAAGT GAAGAGCAGG 
GCTTTCCACA CCAAAGCTTT TCTCGACAAC GATAGCCAGG ATGACACCAA CTACGAGGCT 
CTTGCCAAGT CGTCCCCGAG GAAGCATTCT AAGACCGGTG AGGCCGACGA GGGTTCGGAG 
GACGATTCCA TCGGCAAGAA GGTCAACGCT GCCCCTGTAG TGGCCAAGAA GCGCGGTCGT 
CCTGCCGGCA GTAAAAACAA GGCCACCGAC GACGACGCTG CTGCCCCGAA GCCCAAGCCC 
AAGGTCACCG CCAAGAAGGC TGCTGCTGCT GCTGCTGGAG AGACGGCCTC TGCCGTTCCG 
GCCAAGCTCA AGACGCTGGT CCATCTATCG CCTGCTGCTA AGGCTTATGC CAAGAAGAAC 
CAGAAGGCAA CGAAGCTCGC TAGTGACGAC GAGGATGACC TTGATGGCGA TGACGTCGTA 
ATGAAGGATC CTGCGCCTGC TCCGGCTCGC AGCAGGCCTG GTCGCGCGGC CGCTGCTAGG 
AAGCCGATTG TCATTGATAG CGCAAGTGAG GATGATGATG ACTCGTTTGG GGATATTGGC 
GTGAGCAGCA AGGCAAAGAA GGGTGGTAGG AAGGTGGCTG ATGATGCTTT TGATATGGAA 
GATAGTGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394171.1
CDS	505..6276
Translation	MDSDVESMFEGESDAYSPEVKPKAKKPAAKKAAAPKAPAAKKLTQTTLTGAKAAGKKRPKPDSEDEDGSFGSDKDDGMLDNTPPNAKKQKKAPVKKAAGKPLAEIENDSMQIDSVAPAKKTGAAKTATEMYQKLTQLEHILKRPDTYIGSVERSEQKMWVFNKATGLMENRQISFVPGLYKIFDEILVNAADNSQRDSSMTFLKVTINRESGEISVENNGKGIPVEIHGTEKCYVPELIFGHLLAGSNFDDNEKKTVGGRNGYGAKLTNIFSREFTLECQDSVNGKRYKQTWTDNMSKMSPPKITSNKAADFVRITFRPDFSKFGMADGIDDDLEALLYRRVYDMAGTVSGVKVWLNGEHLKLNFKTYCGLYAKAIAKERDEVADGEDVTPATVIFEQQRSEGKLWEVGFTVSDGSFQQVSFVNNIATTSGGSHVNYIADQIIEVLMKELQKKKGKGHGLKTANVKNQFFIFINCLIDNPAFSSQTKEQLTTKPAKFGSKCQLGDLFLKKVRQSEAIDNLLSFADKKRDKDLAKNDGSKRKRISNDKLIEANYAGGRYSQECTLILTEGDSARGLAVAGRAILDPNRIGVFPLRGKMLNVRDASADQILKNKEVENIKKFLGLKHGKVYTDTKDLRYGHLMIMADQDLDGSHIKGLLINFFECQFPSLLRIPNFFQEFITPVVKVWQGPNPKKPQRLQGFFTLPQYEEWKEAHRNELRRWKYKYLKGLGSSTTDDAQLYFTNLNKHLKEFDVMTREESEMFELAFSKKKADARKEWLAKCEPGTYLDHSTEKISYTDFVNRELILFSMADNIRSIPSMVDGLKPGQRKVLFGCFKQNLIHDQKVVELAGYVSKEAAYHHGEQSLQQTIIGLAQNFVGSNNINCLEPSGNFGSRLSGGSDAASARYIHTRLSPLARKIFHPLDEPNLEFQFDDGKLIEPKVYAPIIPMVLVNGADGIGTGWSTSIPNYHPLDIVENLKRRMGRDSSGDGEEKPFEPMTPWFRGWKGTPEPDGPNRFKFNGIVEVNPQNPNEVDVTELPIRMWTDDFKSKLEEIIRGDKSPSWIKDYKEYNDHQTVHFIIALEEKHMSSALRDGLIEKFKLTKTVATTNLVAFDTRGKIHKYENPQEIMEEYYHYRLNLYGERKKHWLKVYHADYRKLQNQYRFISEIIENKLVVSKKKKSVLVQELRERKYEAFPPKSDGRNVKSPDEELGAADNEDEEDAVGGARDYDYLLSMPIWSLTFERLDKLKQQIAAKKAEHDELDALSEKDLWCRDLDAFTAEWEEQLRLEDEVRKEIRQKGRRVSYKGGAVSRLKKSRGAKDDEDFDPGAKKAKAKAAPKAETIKTQQRFLEKFSGMAKPKPKSTAFTDGADDLSDDDFDLLNKKAPAAAKKKDEDAEPVTTIASVRAKRATVAKPKTYALSDEDSDDEFLDIAKVTTTSEKTTTSASASVSAAEQSEQPVAKPARRAAGAKKPIVDEDSESDLDDFIDDESEEEVIPKPTTKRATAAKKPALEDEDSDVEFQDAPEEPIPPTPKPEAKKATAKKPIVVEDSDEDMMLSEAEKPAHAAKPPTKRAAAVKKSPVLEDDDFESDADDKILSEAEKPAPAKATAKRAAAAKKSPVLEDDDLESDGDDKMVSEAEKPAPANPRKRAAAAKAKSFFIDEEDSESEASDDDDMLLDVGSLVKGIGAPAATSEGPRLSLFSMSRPEGEGSGIGLPKVKSRAFHTKAFLDNDSQDDTNYEALAKSSPRKHSKTGEADEGSEDDSIGKKVNAAPVVAKKRGRPAGSKNKATDDDAAAPKPKPKVTAKKAAAAAAGETASAVPAKLKTLVHLSPAAKAYAKKNQKATKLASDDEDDLDGDDVVMKDPAPAPARSRPGRAAAARKPIVIDSASEDDDDSFGDIGVSSKAKKGGRKVADDAFDMEDSE

Target ORF information:

RefSeq Version	XM_011395869.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A DNA topoisomerase 2, variant (NCU06338), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395869.1

ORF Insert Sequence:

ATGGACTCCG ACGTTGAGTC CATGTTCGAG GGGGAGTCCG ACGCCTATTC ACCAGAGGTG 
AAGCCCAAGG CCAAGAAACC CGCCGCCAAA AAGGCTGCCG CACCAAAGGC GCCCGCCGCA 
AAGAAGTTGA CCCAGACCAC TCTCACCGGC GCAAAGGCCG CTGGCAAGAA GCGACCAAAA 
CCCGACAGCG AGGATGAGGA CGGATCCTTT GGTAGCGACA AGGACGACGG CATGCTCGAC 
AACACCCCTC CCAATGCCAA AAAGCAGAAG AAGGCGCCGG TCAAGAAAGC CGCCGGCAAG 
CCTCTGGCCG AAATCGAGAA CGATAGTATG CAGATCGATA GTGTCGCTCC CGCCAAAAAG 
ACGGGCGCTG CAAAGACGGC CACCGAAATG TACCAAAAAC TTACTCAGCT CGAACATATC 
CTCAAGCGTC CCGACACCTA CATTGGCTCC GTCGAGCGCT CCGAGCAAAA GATGTGGGTT 
TTCAACAAGG CTACCGGTCT CATGGAAAAC CGCCAAATCT CCTTCGTTCC CGGTCTCTAC 
AAGATTTTCG ACGAGATTCT CGTCAACGCC GCCGATAACA GCCAGAGAGA TAGCTCCATG 
ACCTTCCTCA AAGTGACAAT AAATCGCGAG TCTGGCGAAA TCTCGGTCGA GAATAACGGC 
AAGGGCATTC CCGTAGAGAT TCACGGCACC GAAAAATGCT ACGTCCCCGA GCTCATCTTC 
GGTCATCTCC TGGCTGGTTC CAACTTCGAT GACAACGAAA AGAAGACGGT CGGCGGTCGC 
AACGGTTACG GTGCCAAGCT CACAAACATC TTCAGCCGAG AATTCACGCT TGAGTGCCAG 
GATTCCGTCA ACGGCAAGAG ATACAAGCAG ACATGGACCG ACAACATGAG CAAGATGTCA 
CCACCCAAAA TCACCTCCAA CAAGGCTGCT GATTTTGTAC GCATCACTTT CCGCCCCGAC 
TTCAGCAAGT TCGGCATGGC TGACGGGATC GACGATGACC TGGAAGCCTT GCTCTACCGA 
CGTGTTTACG ACATGGCAGG TACTGTTAGT GGTGTCAAGG TTTGGCTTAA TGGTGAACAC 
CTCAAGTTGA ACTTCAAGAC ATACTGCGGG CTGTACGCCA AGGCCATTGC CAAGGAGCGT 
GACGAGGTAG CTGACGGCGA AGATGTCACT CCTGCCACAG TCATCTTTGA GCAGCAAAGG 
TCCGAGGGTA AACTATGGGA AGTGGGCTTT ACGGTTTCGG ATGGCTCATT CCAGCAAGTC 
TCGTTCGTCA ACAATATTGC CACCACCTCT GGCGGCTCCC ACGTCAATTA CATTGCCGAT 
CAGATCATCG AAGTCCTCAT GAAGGAGCTG CAAAAGAAGA AGGGCAAGGG ACACGGTCTC 
AAGACGGCAA ACGTCAAGAA CCAGTTCTTC ATCTTCATCA ACTGCCTGAT TGACAACCCA 
GCCTTCAGCT CGCAGACAAA GGAGCAGTTG ACGACCAAGC CCGCCAAATT CGGCAGCAAG 
TGCCAGCTTG GTGACCTGTT CCTCAAGAAA GTGCGACAAT CGGAGGCCAT TGACAACTTG 
TTGAGCTTTG CCGACAAGAA GCGAGACAAG GATTTGGCTA AGAACGATGG CAGCAAGCGA 
AAGCGTATCA GCAACGACAA GCTGATCGAG GCCAATTATG CTGGTGGAAG ATATTCGCAA 
GAGTGTACCC TTATTCTCAC CGAAGGTGAT TCCGCCAGAG GTCTTGCTGT CGCTGGTCGT 
GCTATCTTGG ATCCTAACCG TATCGGTGTC TTTCCCTTGC GTGGTAAGAT GCTGAACGTC 
CGTGACGCCA GCGCTGATCA AATCTTGAAG AACAAGGAAG TCGAAAACAT CAAGAAGTTC 
CTTGGCCTCA AACACGGAAA GGTCTACACC GATACGAAGG ATTTGCGATA CGGCCATCTG 
ATGATCATGG CTGATCAGGA TCTTGACGGT AGTCATATCA AGGGTCTTCT CATCAACTTT 
TTCGAGTGCC AGTTTCCATC ACTCCTGCGC ATTCCTAATT TCTTCCAGGA GTTCATCACG 
CCCGTTGTCA AGGTGTGGCA AGGTCCTAAC CCGAAGAAGC CTCAAAGGCT ACAGGGTTTC 
TTCACTCTGC CTCAGTACGA GGAGTGGAAG GAAGCACACA GGAACGAATT GCGCAGATGG 
AAGTACAAGT ATCTCAAGGG TTTGGGTAGT TCGACAACAG ACGACGCCCA GCTCTACTTC 
ACCAACTTGA ACAAGCATCT CAAAGAGTTC GATGTCATGA CTAGAGAGGA ATCCGAGATG 
TTCGAGTTGG CCTTCTCCAA GAAGAAGGCC GACGCTCGTA AAGAGTGGCT TGCCAAGTGC 
GAGCCGGGCA CCTACCTCGA CCATTCGACC GAGAAGATCT CCTACACTGA TTTCGTTAAT 
CGCGAACTCA TTCTCTTCAG TATGGCAGAC AACATTCGAT CGATCCCGTC CATGGTGGAT 
GGTCTGAAGC CCGGTCAACG CAAGGTTCTG TTCGGCTGTT TCAAGCAAAA CCTCATTCAC 
GATCAAAAGG TTGTCGAGTT GGCCGGTTAT GTGTCCAAGG AGGCAGCCTA CCACCACGGT 
GAACAGTCTC TGCAGCAAAC GATTATTGGT CTGGCGCAGA ACTTTGTCGG TTCAAACAAC 
ATCAACTGTC TCGAGCCTAG CGGTAACTTC GGTTCGCGTC TTTCCGGTGG TTCCGATGCT 
GCCAGCGCTC GTTACATCCA CACGAGACTG TCTCCCTTGG CGCGAAAGAT CTTTCACCCT 
CTCGATGAGC CCAATCTTGA ATTCCAGTTC GACGATGGCA AACTGATTGA GCCCAAGGTC 
TATGCTCCCA TTATTCCCAT GGTTTTGGTT AACGGTGCAG ATGGTATCGG TACCGGTTGG 
AGCACTTCCA TTCCCAACTA CCACCCTCTT GACATTGTCG AGAACTTGAA GCGTCGCATG 
GGCAGGGACT CATCTGGTGA CGGGGAGGAG AAGCCCTTCG AACCCATGAC TCCGTGGTTC 
CGTGGTTGGA AGGGCACTCC TGAGCCAGAC GGACCCAACA GATTCAAGTT CAACGGTATC 
GTGGAGGTCA ACCCTCAGAA CCCCAATGAG GTTGATGTTA CGGAGTTGCC CATTCGCATG 
TGGACCGATG ATTTCAAGTC CAAGCTGGAA GAGATTATCC GTGGTGATAA GAGTCCTTCA 
TGGATCAAGG ACTACAAAGA GTACAACGAC CACCAAACTG TCCACTTCAT CATCGCCCTT 
GAGGAGAAGC ACATGTCCTC GGCTCTTCGG GACGGTCTCA TTGAAAAGTT CAAGCTTACC 
AAGACAGTGG CCACAACAAA TCTAGTGGCC TTCGACACCC GTGGTAAAAT CCACAAGTAT 
GAGAACCCGC AAGAAATCAT GGAGGAATAC TACCACTACC GACTCAACCT GTACGGTGAG 
CGCAAGAAGC ACTGGCTCAA GGTTTACCAT GCAGACTACC GCAAGCTGCA GAATCAATAC 
CGATTTATTT CGGAAATCAT TGAGAACAAG CTCGTCGTTT CCAAAAAGAA GAAGTCGGTC 
CTTGTCCAGG AACTTCGTGA ACGCAAGTAC GAGGCCTTCC CTCCGAAATC AGATGGAAGG 
AATGTGAAGT CTCCTGATGA GGAGCTCGGT GCTGCGGACA ACGAGGACGA GGAGGATGCC 
GTTGGCGGAG CCCGCGATTA TGACTATCTC CTTTCGATGC CCATTTGGTC GTTGACCTTT 
GAGCGCCTCG ACAAGCTCAA GCAGCAGATT GCTGCAAAGA AGGCTGAGCA CGATGAACTC 
GATGCACTCA GCGAAAAGGA TCTGTGGTGC AGAGATCTCG ATGCTTTCAC CGCTGAGTGG 
GAAGAGCAGT TGAGGCTGGA AGATGAAGTC CGGAAAGAGA TCAGGCAGAA GGGACGCCGT 
GTCTCGTACA AGGGCGGTGC TGTCTCCAGA CTCAAGAAGT CCAGAGGTGC CAAGGATGAC 
GAGGACTTCG ACCCGGGTGC CAAGAAGGCC AAAGCCAAGG CAGCTCCCAA GGCTGAGACA 
ATCAAGACAC AGCAGCGTTT TCTAGAGAAG TTCTCTGGGA TGGCGAAACC CAAGCCTAAG 
TCGACCGCTT TCACCGACGG AGCTGATGAC CTCTCAGACG ATGACTTCGA CTTGTTGAAC 
AAGAAGGCCC CGGCTGCAGC CAAAAAGAAG GATGAGGATG CGGAGCCCGT TACTACCATC 
GCCAGTGTGC GCGCCAAGCG TGCCACCGTA GCGAAGCCTA AGACTTATGC GCTCAGTGAT 
GAAGATTCGG ACGATGAGTT CTTGGACATC GCAAAGGTTA CCACAACTTC TGAGAAAACG 
ACCACGTCGG CTTCAGCTTC GGTTTCAGCG GCAGAACAAT CTGAGCAACC TGTAGCAAAG 
CCCGCGAGGC GTGCTGCAGG AGCCAAGAAG CCTATCGTTG ATGAGGATTC TGAATCCGAT 
TTGGATGACT TCATTGACGA CGAGTCCGAG GAAGAGGTGA TTCCTAAGCC CACCACCAAG 
CGAGCCACCG CTGCCAAGAA GCCGGCCCTC GAAGATGAAG ACTCCGATGT CGAGTTCCAG 
GACGCTCCTG AAGAGCCTAT CCCGCCCACC CCCAAGCCGG AGGCCAAGAA GGCTACGGCC 
AAGAAGCCAA TTGTAGTTGA AGACTCTGAC GAAGACATGA TGCTCAGCGA AGCAGAGAAG 
CCTGCGCACG CAGCTAAGCC CCCCACTAAG CGCGCTGCTG CTGTCAAGAA ATCACCTGTT 
CTCGAAGATG ATGACTTCGA GAGCGACGCT GATGACAAGA TTTTGAGCGA GGCAGAAAAA 
CCTGCGCCGG CTAAGGCCAC CGCCAAGCGT GCGGCTGCTG CCAAGAAGTC ACCTGTTCTT 
GAGGACGATG ATCTTGAGAG CGACGGTGAT GACAAGATGG TAAGCGAGGC TGAGAAACCC 
GCTCCGGCCA ATCCCAGGAA GCGCGCGGCG GCCGCTAAGG CCAAGTCCTT CTTCATAGAC 
GAAGAAGACT CCGAAAGCGA AGCTTCTGAT GACGACGACA TGCTTCTTGA CGTCGGCTCC 
CTAGTCAAGG GTATAGGAGC CCCTGCCGCG ACGAGCGAGG GCCCGAGGCT CAGCCTCTTT 
TCCATGTCTC GGCCAGAAGG CGAGGGTAGC GGTATCGGCC TGCCCAAAGT GAAGAGCAGG 
GCTTTCCACA CCAAAGCTTT TCTCGACAAC GATAGCCAGG ATGACACCAA CTACGAGGCT 
CTTGCCAAGT CGTCCCCGAG GAAGCATTCT AAGACCGGTG AGGCCGACGA GGGTTCGGAG 
GACGATTCCA TCGGCAAGAA GGTCAACGCT GCCCCTGTAG TGGCCAAGAA GCGCGGTCGT 
CCTGCCGGCA GTAAAAACAA GGCCACCGAC GACGACGCTG CTGCCCCGAA GCCCAAGCCC 
AAGGTCACCG CCAAGAAGGC TGCTGCTGCT GCTGCTGGAG AGACGGCCTC TGCCGTTCCG 
GCCAAGCTCA AGACGCTGGT CCATCTATCG CCTGCTGCTA AGGCTTATGC CAAGAAGAAC 
CAGAAGGCAA CGAAGCTCGC TAGTGACGAC GAGGATGACC TTGATGGCGA TGACGTCGTA 
ATGAAGGATC CTGCGCCTGC TCCGGCTCGC AGCAGGCCTG GTCGCGCGGC CGCTGCTAGG 
AAGCCGATTG TCATTGATAG CGCAAGTGAG GATGATGATG ACTCGTTTGG GGATATTGGC 
GTGAGCAGCA AGGCAAAGAA GGGTGGTAGG AAGGTGGCTG ATGATGCTTT TGATATGGAA 
GATAGTGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU06338 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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