top2b cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following top2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa45162 XM_018095006.1
Latest version!
Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa60892 XM_002932410.5
Latest version!
Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa60893 XM_018095006.2
Latest version!
Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa11913 XM_002932410.4
Latest version!
Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa45162
    Clone ID Related Accession (Same CDS sequence) XM_018095006.1
    Accession Version XM_018095006.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
    ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT
    GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT
    ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG
    GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC
    AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG
    AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT
    AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT
    GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA
    AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT
    TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT
    GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT
    TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA
    GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT
    CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT
    GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA
    TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT
    GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG
    AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT
    TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA
    CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA
    TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG
    CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT
    GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA
    GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT
    GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG
    GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT
    GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG
    GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA
    TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG
    AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA
    GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG
    GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC
    CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA
    GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG
    CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG
    CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA
    AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC
    AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA
    GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC
    TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC
    CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT
    GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT
    CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT
    AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT
    CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG
    GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA
    ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC
    ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA
    GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT
    GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA
    TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT
    TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA
    GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA
    ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT
    GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC
    CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT
    AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC
    AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT
    CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA
    AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC
    AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG
    CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT
    GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA
    GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA
    GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC
    AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG
    GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT
    GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC
    GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT
    TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG
    GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA
    CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGAATCT
    GCAAAATATG ACAGTGATGA TGATGATGAT GATGATGAAC CCTCCCCCCT TTACTCCTCA
    TATTCCTTCA AATCTACAGA AAAGCCAGCC AGCAAAGGAT CTAGTGAGAA AGAATCCACC
    AAATATAACA GTGACGATGA GGATGATGAC GATGACGATG TACCGTTTTC GTCGTTTACC
    TTTAAATCTT CCGAAAAGCC AGCCAGTAAA GGTTCTGCTA AGAAAACAAA ATCTTTGTCA
    GATGGTACTC CTAAACCAAA ACGACCGCCA AAACCAAAGA AGTCTGAGAC TTCATTGATT
    TCCGACTCTG ACTTGTTAGA TTTTGGTGCA TCAAAGAAAT CTGCAACACC CAAAGCAAAA
    GGAAGGGGAA GGAAACGAAA AACATCTGAT TCTGAAAATG AAGGAGATTA CAATCCAGGG
    CGAAAACCTA CAAAACCTGC ATCTTCTAGC AAAAAATCCA AGAAAGCATC ATTTGAGCAG
    GATTCAGATG GTGATCTCTT CCCTTCAGAA GTCGGCTCTG AGACCGCATC GAGGCAAAGG
    CCTGGCCGGG CTAGGAAAGA AGTCAAATAT TTTGCAGACT CTGACGATGA AGATGATTTT
    GCTATGTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950495.1
    CDS840..5831
    Translation

    Target ORF information:

    RefSeq Version XM_018095006.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
    ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT
    GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT
    ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG
    GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC
    AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG
    AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT
    AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT
    GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA
    AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT
    TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT
    GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT
    TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA
    GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT
    CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT
    GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA
    TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT
    GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG
    AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT
    TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA
    CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA
    TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG
    CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT
    GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA
    GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT
    GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG
    GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT
    GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG
    GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA
    TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG
    AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA
    GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG
    GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC
    CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA
    GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG
    CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG
    CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA
    AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC
    AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA
    GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC
    TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC
    CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT
    GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT
    CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT
    AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT
    CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG
    GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA
    ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC
    ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA
    GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT
    GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA
    TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT
    TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA
    GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA
    ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT
    GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC
    CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT
    AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC
    AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT
    CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA
    AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC
    AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG
    CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT
    GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA
    GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA
    GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC
    AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG
    GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT
    GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC
    GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT
    TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG
    GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA
    CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGAATCT
    GCAAAATATG ACAGTGATGA TGATGATGAT GATGATGAAC CCTCCCCCCT TTACTCCTCA
    TATTCCTTCA AATCTACAGA AAAGCCAGCC AGCAAAGGAT CTAGTGAGAA AGAATCCACC
    AAATATAACA GTGACGATGA GGATGATGAC GATGACGATG TACCGTTTTC GTCGTTTACC
    TTTAAATCTT CCGAAAAGCC AGCCAGTAAA GGTTCTGCTA AGAAAACAAA ATCTTTGTCA
    GATGGTACTC CTAAACCAAA ACGACCGCCA AAACCAAAGA AGTCTGAGAC TTCATTGATT
    TCCGACTCTG ACTTGTTAGA TTTTGGTGCA TCAAAGAAAT CTGCAACACC CAAAGCAAAA
    GGAAGGGGAA GGAAACGAAA AACATCTGAT TCTGAAAATG AAGGAGATTA CAATCCAGGG
    CGAAAACCTA CAAAACCTGC ATCTTCTAGC AAAAAATCCA AGAAAGCATC ATTTGAGCAG
    GATTCAGATG GTGATCTCTT CCCTTCAGAA GTCGGCTCTG AGACCGCATC GAGGCAAAGG
    CCTGGCCGGG CTAGGAAAGA AGTCAAATAT TTTGCAGACT CTGACGATGA AGATGATTTT
    GCTATGTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60892
    Clone ID Related Accession (Same CDS sequence) XM_002932410.5
    Accession Version XM_002932410.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5100bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932410.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
    TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT
    AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT
    GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC
    ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT
    TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA
    AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT
    AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA
    ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA
    GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG
    ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG
    ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG
    CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA
    AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG
    AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT
    GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT
    TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA
    AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT
    GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT
    TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC
    ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA
    GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA
    ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG
    CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT
    GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG
    TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG
    ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA
    AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA
    GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT
    TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT
    ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA
    CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT
    TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT
    GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC
    AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG
    CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC
    AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT
    GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG
    GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA
    GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT
    GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT
    GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT
    GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG
    TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG
    GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG
    GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG
    GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA
    ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG
    GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT
    CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA
    GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG
    GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG
    GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG
    TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA
    GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA
    GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG
    GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG
    GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG
    CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG
    CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT
    CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA
    AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA
    GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG
    AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA
    CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC
    AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA
    TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT
    GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA
    GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT
    GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG
    CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA
    GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA
    GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA
    GATTCTGCAA TATATAACAG TGACGATGAT GAACCCTCCC CACTTTACTC TTCGTACTCC
    TTCAAAACTT CAGAGAAAAC AACAGGCAAA GGATCTAGTA AAAAAGAATC TGCAAAATAT
    GACAGTGATG ATGATGATGA TGATGAACCC TCCCCCCTTT ACTCCTCATA TTCCTTCAAA
    TCTACAGAAA AGCCAGCCAG CAAAGGATCT AGTGAGAAAG AATCCACCAA ATATAACAGT
    GACGATGAGG ATGATGACGA TGACGATGTA CCGTTTTCGT CGTTTACCTT TAAATCTTCC
    GAAAAGCCAG CCAGTAAAGG TTCTGCTAAG AAAACAAAAT CTTTGTCAGA TGGTACTCCT
    AAACCAAAAC GACCGCCAAA ACCAAAGAAG TCTGAGACTT CATTGATTTC CGACTCTGAC
    TTGTTAGATT TTGGTGCATC AAAGAAATCT GCAACACCCA AAGCAAAAGG AAGGGGAAGG
    AAACGAAAAA CATCTGATTC TGAAAATGAA GGAGATTACA ATCCAGGGCG AAAACCTACA
    AAACCTGCAT CTTCTAGCAA AAAATCCAAG AAAGCATCAT TTGAGCAGGA TTCAGATGGT
    GATCTCTTCC CTTCAGAAGT CGGCTCTGAG ACCGCATCGA GGCAAAGGCC TGGCCGGGCT
    AGGAAAGAAG TCAAATATTT TGCAGACTCT GACGATGAAG ATGATTTTGC TATGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002932456.3
    CDS872..5971
    Translation

    Target ORF information:

    RefSeq Version XM_002932410.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002932410.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
    TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT
    AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT
    GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC
    ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT
    TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA
    AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT
    AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA
    ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA
    GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG
    ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG
    ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG
    CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA
    AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG
    AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT
    GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT
    TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA
    AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT
    GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT
    TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC
    ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA
    GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA
    ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG
    CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT
    GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG
    TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG
    ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA
    AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA
    GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT
    TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT
    ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA
    CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT
    TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT
    GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC
    AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG
    CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC
    AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT
    GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG
    GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA
    GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT
    GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT
    GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT
    GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG
    TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG
    GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG
    GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG
    GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA
    ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG
    GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT
    CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA
    GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG
    GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG
    GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG
    TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA
    GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA
    GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG
    GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG
    GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG
    CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG
    CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT
    CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA
    AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA
    GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG
    AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA
    CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC
    AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA
    TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT
    GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA
    GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT
    GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG
    CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA
    GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA
    GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA
    GATTCTGCAA TATATAACAG TGACGATGAT GAACCCTCCC CACTTTACTC TTCGTACTCC
    TTCAAAACTT CAGAGAAAAC AACAGGCAAA GGATCTAGTA AAAAAGAATC TGCAAAATAT
    GACAGTGATG ATGATGATGA TGATGAACCC TCCCCCCTTT ACTCCTCATA TTCCTTCAAA
    TCTACAGAAA AGCCAGCCAG CAAAGGATCT AGTGAGAAAG AATCCACCAA ATATAACAGT
    GACGATGAGG ATGATGACGA TGACGATGTA CCGTTTTCGT CGTTTACCTT TAAATCTTCC
    GAAAAGCCAG CCAGTAAAGG TTCTGCTAAG AAAACAAAAT CTTTGTCAGA TGGTACTCCT
    AAACCAAAAC GACCGCCAAA ACCAAAGAAG TCTGAGACTT CATTGATTTC CGACTCTGAC
    TTGTTAGATT TTGGTGCATC AAAGAAATCT GCAACACCCA AAGCAAAAGG AAGGGGAAGG
    AAACGAAAAA CATCTGATTC TGAAAATGAA GGAGATTACA ATCCAGGGCG AAAACCTACA
    AAACCTGCAT CTTCTAGCAA AAAATCCAAG AAAGCATCAT TTGAGCAGGA TTCAGATGGT
    GATCTCTTCC CTTCAGAAGT CGGCTCTGAG ACCGCATCGA GGCAAAGGCC TGGCCGGGCT
    AGGAAAGAAG TCAAATATTT TGCAGACTCT GACGATGAAG ATGATTTTGC TATGTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa60893
    Clone ID Related Accession (Same CDS sequence) XM_018095006.2
    Accession Version XM_018095006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018095006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
    TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT
    AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT
    GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC
    ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT
    TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA
    AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT
    AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA
    ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA
    GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG
    ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG
    ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG
    CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA
    AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG
    AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT
    GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT
    TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA
    AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT
    GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT
    TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC
    ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA
    GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA
    ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG
    CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT
    GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG
    TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG
    ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA
    AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA
    GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT
    TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT
    ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA
    CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT
    TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT
    GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC
    AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG
    CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC
    AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT
    GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG
    GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA
    GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT
    GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT
    GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT
    GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG
    TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG
    GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG
    GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG
    GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA
    ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG
    GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT
    CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA
    GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG
    GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG
    GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG
    TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA
    GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA
    GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG
    GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG
    GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG
    CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG
    CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT
    CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA
    AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA
    GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG
    AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA
    CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC
    AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA
    TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT
    GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA
    GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT
    GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG
    CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA
    GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA
    GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA
    GAATCTGCAA AATATGACAG TGATGATGAT GATGATGATG AACCCTCCCC CCTTTACTCC
    TCATATTCCT TCAAATCTAC AGAAAAGCCA GCCAGCAAAG GATCTAGTGA GAAAGAATCC
    ACCAAATATA ACAGTGACGA TGAGGATGAT GACGATGACG ATGTACCGTT TTCGTCGTTT
    ACCTTTAAAT CTTCCGAAAA GCCAGCCAGT AAAGGTTCTG CTAAGAAAAC AAAATCTTTG
    TCAGATGGTA CTCCTAAACC AAAACGACCG CCAAAACCAA AGAAGTCTGA GACTTCATTG
    ATTTCCGACT CTGACTTGTT AGATTTTGGT GCATCAAAGA AATCTGCAAC ACCCAAAGCA
    AAAGGAAGGG GAAGGAAACG AAAAACATCT GATTCTGAAA ATGAAGGAGA TTACAATCCA
    GGGCGAAAAC CTACAAAACC TGCATCTTCT AGCAAAAAAT CCAAGAAAGC ATCATTTGAG
    CAGGATTCAG ATGGTGATCT CTTCCCTTCA GAAGTCGGCT CTGAGACCGC ATCGAGGCAA
    AGGCCTGGCC GGGCTAGGAA AGAAGTCAAA TATTTTGCAG ACTCTGACGA TGAAGATGAT
    TTTGCTATGT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950495.2
    CDS872..5866
    Translation

    Target ORF information:

    RefSeq Version XM_018095006.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DNA topoisomerase II beta (top2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCTAAAT CTGGCGGCGG CGGCGGCGGA GGAGCTGGAG GAGGGAGCGG AGGGCTCACC 
    TGGGTGACAC TTTTTGATAA TCAGAACGCA TCAAAAAAGG AAGAAACAGA AAGCAATAAT
    AAAAATGACT CCTCCAAGAA GATGTCCGTA GAAAGAGTTT ACCAGAAAAA GACCCAGCTT
    GAGCATATTC TTCTGCGACC TGACACATAC ATTGGATCTG TGGAGACAGT TACTCAGTCC
    ATGTGGGTTT ATGATGAAGA TGTTGGAATG AATTGCCGTG ACATAACCTA TGTGCCCGGT
    TTATACAAGA TATTTGATGA AATCCTTGTA AACGCAGCTG ATAATAAACA GAGAGATAAA
    AATATGAACT GCATAAAAAT TTCTATTGAT CCTGATTCTA ACATCATCAG CATTTGGAAT
    AATGGTAAAG GAATACCTGT GGTTGAGCAC AAAGTGGAGA AAATGTATGT CCCTGCTTTA
    ATTTTTGGCC AGTTATTAAC ATCAAGCAAC TATGATGATG AGGAAAAGAA AGTTACAGGA
    GGGCGAAATG GATATGGTGC TAAACTTTGC AATATATTCA GCACAAAATT TACTGTGGAG
    ACAGCTTGTA AGGAATACAA GCATAGCTTT AAGCAGACAT GGACAAACAA CATGTTAAAG
    ACAACTGAAC CCAAAATTAA ACATTTTGAT GGAGATGACT ACACATGCAT AACATTCCAG
    CCAGATTTAG CCAAGTTCCA AATGGAAAAA CTGGACAAGG ATACTGTGGC TCTTCTGACA
    AGACGAGCAT ATGATATTGC GGGTTCCTGC AAAGGAGTCA AAGTCTTGCT CAATGGGAAG
    AAGCTTCCGG TAAATGGCTT CCGCAGTTAC GTAGATCTTT ACGTAAAGGA CAGACTTGAT
    GAAACTGGTG TCGCTCTTAA AGTAATCCAT GAAGTTGCAA ATGAAAGATG GGATGTATGT
    TTAACATTGA GCGAAAAGGG ATTCCAACAA ATCAGTTTTG TGAACAGTAT TGCTACCACA
    AAGGGTGGTA GACATGTAGA TTATGTAGTA GACCAAATTG TGTCAAAACT CATTGAAGTT
    GTGAAGAAGA AGAATAAAGC TGGAGTTTCT GTAAAGCCCT TCCAGGTGAA GAATCACGTT
    TGGGTTTTTG TCAACTGTTT AATAGAAAAC CCAACTTTTG ATTCTCAGAC AAAAGAAAAC
    ATGACACTAC AGCCTAAAGG TTTTGGGTCC AAATGCCAGC TGTCAGAAAA ATTTTCAAAA
    GCGGCATCTA ACTGTGGCAT AGTCGAGAGT ATTCTAAATT GGGTTAAATT CAAGGCCCAA
    ACACAGCTAA ATAAGAAATG CTCTTCTGTG AAACACAGTA AAATCAAAGG CATTCCCAAG
    CTGGATGATG CTAATGATGC AGGCGGAAAA CATTCCCTGG ACTGCACTCT GATATTAACT
    GAGGGAGACT CTGCCAAATC TTTAGCTGTT TCAGGCTTAG GTGTCATTGG CCGAGATCGG
    TACGGTGTAT TCCCGCTCAG GGGAAAAATT CTCAATGTTC GAGAAGCTTC CCACAAACAG
    ATTATGGAAA ATGCTGAGAT AAACAACATC ATTAAAATAG TTGGCTTGCA GTACAAAAAA
    AGCTATGATG ATAATGAATC CCTTAAAACC TTGCGTTATG GAAAAATTAT GATAATGACA
    GATCAGGATC AAGATGGCTC GCATATCAAG GGCTTATTGA TTAATTTTTT CCATCACAAT
    TGGCCATCTC TACTGAAACA TAGTTTCCTT GAAGAATTTA TCACACCCAT TGTTAAGGCT
    ACAAAGAATA AGCAGGAGCT TTCATTCTAC AGTATTCCTG AGTTTGAAGA ATGGAAAAAA
    CAAACAGAAA ACCAAAAATT GTGGAAGATT AAGTATTACA AAGGTTTGGG CACCAGCACT
    TCGAAGGAAG CAAAGGAATA CTTTGCTGAC ATGGACAGGC ACAGAATTAT ATTTAGATAT
    GCAGGCCCTG AAGATGATGC AGCTATCACT CTGGCTTTCA GCAAAAAGAA AGTAGATGAC
    AGGAAAGAAT GGCTCACAAA CTTTATGGAA GACAGGCGAC AACGTCGACT ACACGGATTG
    CCCGAGCAAT TCTTGTACGG CACAGCTACA AAACACTTGA CTTACAATGA CTTCATTAAC
    AAGGAGCTGA TTCTTTTTTC AAACTCTGAT AATGAGCGAT CTATTCCATC TCTTGTTGAT
    GGTTTAAAGC CTGGGCAAAG AAAAGTTCTG TTTACTTGTT TTAAAAGGAA TGATAAACGG
    GAAGTCAAGG TTGCTCAGCT GGCTGGTTCT GTTGCTGAAA TGTCCGCTTA CCACCATGGA
    GAGCAAGCCT TGATGATGAC AATAGTAAAT CTTGCCCAGA ACTTTGTGGG AAGTAACAAT
    GTGAGCTTGC TGCAGCCCAT TGGTCAGTTT GGTACTCGAC TCCATGGTGG AAAGGATGCT
    GCCAGCCCTC GTTATATATT CACAATGTTA AGTCCTCTAG CAAGATTGCT CTATCCATCT
    GTGGATGATA ACCTTTTGAA GTTCCTTTAT GATGATAACC AGCGAGTAGA ACCAGAGTGG
    TACATTCCTA TTATACCCCT GGTTTTAGTT AATGGAGCAG AAGGAATCGG TACTGGATGG
    GCTTGTAAAC TGCCCAATTA TGACACAAGA GAAATTGTCA ATAATGTGAA GAGAATGCTG
    GATGGTCTTG ATCCTTATCC ATTGCTTCCA AGCTACAAGA ATTTTAAAGG TACGATTCAG
    GAACTGGGAC AGAATCAGTA TGCAATGAGT GGTGAAATAT ATGTGCTGGA CCGGAACACA
    ATTGAAATCA CAGAACTTCC TGTCAGGACA TGGACCCAGG TTTACAAAGA ACAAGTGCTG
    GAGCCCATGC TCAATGGAAC CGAGAAAACA CCATCGTTAA TCTCTGATTA CAAAGAGTAT
    CATACAGACA CAACTGTAAA ATTTGTTGTC AAAATGACAG AAGAAAAGTT GGCACAGGCA
    GAAGCTGCTG GCTTGCATAA AGTCTTTAAA CTTCAAACAA GTCTTACTTG CAATTCTATG
    GTATTATTTG ACCACATGGG CTGTCTAAAG AAATATGAAA CCGTGCAGGA CATTCTGAAG
    GAATTTTTTG ATCTCCGGTT AAATTACTAC ACCCTGCGCA AAGAATGGCT GATAGGAATG
    TTGGGAGCTG AAGCTGCAAG ACTGAACAAC CAAGCCCGGT TTGTCCTGGA GAAGATACAA
    GGCAAAATCT CTATAGAGAA CAAAACCAAA AGAGATCTAA TTCAGATGTT GGTGCAGAAA
    GGATTTGAGT CAGACCCTGT GAAAGCTTGG AAGGAGGCAC AAGAAAAAGC AACAGAGGAG
    GATGACCTTC AAGATCCGAA TGAAGACAAT TCATCCGACT CTGGCACTGG ATCAGGACCG
    GATTTTAACT ACATTCTCAA TATGTCTCTC TGGAGCCTTT CAAAGGAAAA AGTAGATGAG
    CTTGTCAGAC AGAGGGATAT AAAGAGCAAA GAACTGAATG ACCTCAAGAG GAAGTCTCCG
    CCTGATCTGT GGAAGGAAGA CTTGGCAGCT TTTGTTGAAG AGCTAGACAG AGTGGAAGCT
    CAAGAAAAAG AAGATGTCTT AGCAGGGTTT GCAGGCAAAG CAATAAAAGG CAAAATTGGA
    AAACCCAAAA TGAAGAAATT ACAGCTTGAG GAGACTTTGC CATCTCTTTA CGGCAGAAGA
    GTAGAGCCAC AAATCAGTGC AATGAAAGCA GATGCCAGCA AAAAGATGCT AAAGAAAAAG
    AAGGGTGAAA TTGACTCTAT TGCACTGAAA ATGGAATTTG ATGAGGAGTT TAGCTCGGCA
    CCAGCAGAGG GAGGAGCTGA AGATTCCATG AATACCACTC CTGTAAAACC TGTAAAGCCC
    AAGAGAGAAA GAAAAGAACC AGGTCCTCGA GCCAAAAAGA CTGTATTAAC ACCCAAGTCA
    TCAACCAAGA AAGGGAAGAA GCGAAACCCT TGGTCAGACA ATGAGTCAAA ATCAGACAGT
    GACCTGGAAG AAGCTGAGGC AGAGCCTGTG GTCATTCCTC GGGACTCTCT CCTAAGGAGA
    GCAGCTGCTG AAAGACCGAA GTACACGTTT GATTTCTCTG AAGAGGAAGA TGCAGACGAT
    GACGACGAAG GTGTCATTAG CCGTAGTAAT AATAATAATA ATATTAATGA CTTAAAACCG
    CCAGCTTCTC CTGCTGCTAA TAGCCGAGGT GAAGATGATG ATGTTCTCTC TGACAGCCAA
    GAAAAGGATG ACTATGAATT CTCCCCTTTC AAGTCTACCC CAGCCGCACA AAAGCCTTCA
    GAAACACAAA AGAAAAAAGA CTACATATTT TCATCATCTT CATTTTCAGA AAAAGTAGAA
    GAATCTGCAA AATATGACAG TGATGATGAT GATGATGATG AACCCTCCCC CCTTTACTCC
    TCATATTCCT TCAAATCTAC AGAAAAGCCA GCCAGCAAAG GATCTAGTGA GAAAGAATCC
    ACCAAATATA ACAGTGACGA TGAGGATGAT GACGATGACG ATGTACCGTT TTCGTCGTTT
    ACCTTTAAAT CTTCCGAAAA GCCAGCCAGT AAAGGTTCTG CTAAGAAAAC AAAATCTTTG
    TCAGATGGTA CTCCTAAACC AAAACGACCG CCAAAACCAA AGAAGTCTGA GACTTCATTG
    ATTTCCGACT CTGACTTGTT AGATTTTGGT GCATCAAAGA AATCTGCAAC ACCCAAAGCA
    AAAGGAAGGG GAAGGAAACG AAAAACATCT GATTCTGAAA ATGAAGGAGA TTACAATCCA
    GGGCGAAAAC CTACAAAACC TGCATCTTCT AGCAAAAAAT CCAAGAAAGC ATCATTTGAG
    CAGGATTCAG ATGGTGATCT CTTCCCTTCA GAAGTCGGCT CTGAGACCGC ATCGAGGCAA
    AGGCCTGGCC GGGCTAGGAA AGAAGTCAAA TATTTTGCAG ACTCTGACGA TGAAGATGAT
    TTTGCTATGT TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa11913
    Clone ID Related Accession (Same CDS sequence) XM_002932410.4
    Accession Version XM_002932410.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5097bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DNA topoisomerase 2-beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932410.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
    ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT
    GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT
    ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG
    GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC
    AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG
    AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT
    AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT
    GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA
    AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT
    TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT
    GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT
    TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA
    GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT
    CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT
    GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA
    TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT
    GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG
    AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT
    TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA
    CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA
    TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG
    CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT
    GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA
    GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT
    GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG
    GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT
    GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG
    GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA
    TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG
    AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA
    GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG
    GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC
    CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA
    GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG
    CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG
    CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA
    AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC
    AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA
    GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC
    TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC
    CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT
    GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT
    CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT
    AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT
    CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG
    GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA
    ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC
    ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA
    GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT
    GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA
    TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT
    TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA
    GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA
    ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT
    GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC
    CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT
    AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC
    AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT
    CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA
    AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC
    AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG
    CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT
    GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA
    GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA
    GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC
    AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG
    GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT
    GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC
    GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT
    TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG
    GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA
    CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGATTCT
    GCAATATATA ACAGTGACGA TGATGAACCC TCCCCACTTT ACTCTTCGTA CTCCTTCAAA
    ACTTCAGAGA AAACAACAGG CAAAGGATCT AGTAAAAAAG AATCTGCAAA ATATGACAGT
    GATGATGATG ATGATGATGA TGAACCCTCC CCCCTTTACT CCTCATATTC CTTCAAATCT
    ACAGAAAAGC CAGCCAGCAA AGGATCTAGT GAGAAAGAAT CCACCAAATA TAACAGTGAC
    GATGAGGATG ATGACGATGA CGATGTACCG TTTTCGTCGT TTACCTTTAA ATCTTCCGAA
    AAGCCAGCCA GTAAAGGTTC TGCTAAGAAA ACAAAATCTT TGTCAGATGG TACTCCTAAA
    CCAAAACGAC CGCCAAAACC AAAGAAGTCT GAGACTTCAT TGATTTCCGA CTCTGACTTG
    TTAGATTTTG GTGCATCAAA GAAATCTGCA ACACCCAAAG CAAAAGGAAG GGGAAGGAAA
    CGAAAAACAT CTGATTCTGA AAATGAAGGA GATTACAATC CAGGGCGAAA ACCTACAAAA
    CCTGCATCTT CTAGCAAAAA ATCCAAGAAA GCATCATTTG AGCAGGATTC AGATGGTGAT
    CTCTTCCCTT CAGAAGTCGG CTCTGAGACC GCATCGAGGC AAAGGCCTGG CCGGGCTAGG
    AAAGAAGTCA AATATTTTGC AGACTCTGAC GATGAAGATG ATTTTGCTAT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002932456.2
    CDS835..5931
    Translation

    Target ORF information:

    RefSeq Version XM_002932410.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis topoisomerase (DNA) II beta (top2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002932410.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCTAAAT CTGGCGGCGG CGGAGGAGCT GGAGGAGGGA GCGGAGGGCT CACCTGGGTG 
    ACACTTTTTG ATAATCAGAA CGCATCAAAA AAGGAAGAAA CAGAAAGCAA TAATAAAAAT
    GACTCCTCCA AGAAGATGTC CGTAGAAAGA GTTTACCAGA AAAAGACCCA GCTTGAGCAT
    ATTCTTCTGC GACCTGACAC ATACATTGGA TCTGTGGAGA CAGTTACTCA GTCCATGTGG
    GTTTATGATG AAGATGTTGG AATGAATTGC CGTGACATAA CCTATGTGCC CGGTTTATAC
    AAGATATTTG ATGAAATCCT TGTAAACGCA GCTGATAATA AACAGAGAGA TAAAAATATG
    AACTGCATAA AAATTTCTAT TGATCCTGAT TCTAACATCA TCAGCATTTG GAATAATGGT
    AAAGGAATAC CTGTGGTTGA GCACAAAGTG GAGAAAATGT ATGTCCCTGC TTTAATTTTT
    GGCCAGTTAT TAACATCAAG CAACTATGAT GATGAGGAAA AGAAAGTTAC AGGAGGGCGA
    AATGGATATG GTGCTAAACT TTGCAATATA TTCAGCACAA AATTTACTGT GGAGACAGCT
    TGTAAGGAAT ACAAGCATAG CTTTAAGCAG ACATGGACAA ACAACATGTT AAAGACAACT
    GAACCCAAAA TTAAACATTT TGATGGAGAT GACTACACAT GCATAACATT CCAGCCAGAT
    TTAGCCAAGT TCCAAATGGA AAAACTGGAC AAGGATACTG TGGCTCTTCT GACAAGACGA
    GCATATGATA TTGCGGGTTC CTGCAAAGGA GTCAAAGTCT TGCTCAATGG GAAGAAGCTT
    CCGGTAAATG GCTTCCGCAG TTACGTAGAT CTTTACGTAA AGGACAGACT TGATGAAACT
    GGTGTCGCTC TTAAAGTAAT CCATGAAGTT GCAAATGAAA GATGGGATGT ATGTTTAACA
    TTGAGCGAAA AGGGATTCCA ACAAATCAGT TTTGTGAACA GTATTGCTAC CACAAAGGGT
    GGTAGACATG TAGATTATGT AGTAGACCAA ATTGTGTCAA AACTTATTGA AGTTGTGAAG
    AAGAAGAATA AAGCTGGAGT TTCTGTAAAG CCCTTCCAGG TGAAGAATCA CGTTTGGGTT
    TTTGTCAACT GTTTAATAGA AAACCCAACT TTTGATTCTC AGACAAAAGA AAACATGACA
    CTACAGCCTA AAGGTTTTGG GTCCAAATGC CAGCTGTCAG AAAAATTTTC AAAAGCGGCA
    TCTAACTGTG GCATAGTCGA GAGTATTCTA AATTGGGTTA AATTCAAGGC CCAAACACAG
    CTAAATAAGA AATGCTCTTC TGTGAAACAC AGTAAAATCA AAGGCATTCC CAAGCTGGAT
    GATGCTAATG ATGCAGGCGG AAAACATTCC CTGGACTGCA CTCTGATATT AACTGAGGGA
    GACTCTGCCA AATCTTTAGC TGTTTCAGGC TTAGGTGTCA TTGGCCGAGA TCGGTACGGT
    GTATTCCCGC TCAGGGGAAA AATTCTCAAT GTTCGAGAAG CTTCCCACAA ACAGATTATG
    GAAAATGCTG AGATAAACAA CATCATTAAA ATAGTTGGCT TGCAGTACAA AAAAAGCTAT
    GATGATAATG AATCCCTTAA AACCTTGCGT TATGGAAAAA TTATGATAAT GACAGATCAG
    GATCAAGATG GCTCGCATAT CAAGGGCTTA TTGATTAATT TTTTCCATCA CAATTGGCCA
    TCTCTACTGA AACATAGTTT CCTTGAAGAA TTTATCACAC CCATTGTTAA GGCTACAAAG
    AATAAGCAGG AGCTTTCATT CTACAGTATT CCTGAGTTTG AAGAATGGAA AAAACAAACA
    GAAAACCAAA AATTGTGGAA GATTAAGTAT TACAAAGGTT TGGGCACCAG CACTTCGAAG
    GAAGCAAAGG AATACTTTGC TGACATGGAC AGGCACAGAA TTATATTTAG ATATGCAGGC
    CCTGAAGATG ATGCAGCTAT CACTCTGGCT TTCAGCAAAA AGAAAGTAGA TGACAGGAAA
    GAATGGCTCA CAAACTTTAT GGAAGACAGG CGACAACGTC GACTACACGG ATTGCCCGAG
    CAATTCTTGT ACGGCACAGC TACAAAACAC TTGACTTACA ATGACTTCAT TAACAAGGAG
    CTGATTCTTT TTTCAAACTC TGATAATGAG CGATCTATTC CATCTCTTGT TGATGGTTTA
    AAGCCTGGGC AAAGAAAAGT TCTGTTTACT TGTTTTAAAA GGAATGATAA ACGGGAAGTC
    AAGGTTGCTC AGCTGGCTGG TTCTGTTGCT GAAATGTCCG CTTACCACCA TGGAGAGCAA
    GCCTTGATGA TGACAATAGT AAATCTTGCC CAGAACTTTG TGGGAAGTAA CAATGTGAGC
    TTGCTGCAGC CCATTGGTCA GTTTGGTACT CGACTCCATG GTGGAAAGGA TGCTGCCAGC
    CCTCGTTATA TATTCACAAT GTTAAGTCCT CTAGCAAGAT TGCTCTATCC ATCTGTGGAT
    GATAACCTTT TGAAGTTCCT TTATGATGAT AACCAGCGAG TAGAACCAGA GTGGTACATT
    CCTATTATAC CCCTGGTTTT AGTTAATGGA GCAGAAGGAA TCGGTACTGG ATGGGCTTGT
    AAACTGCCCA ATTATGACAC AAGAGAAATT GTCAATAATG TGAAGAGAAT GCTGGATGGT
    CTTGATCCTT ATCCATTGCT TCCAAGCTAC AAGAATTTTA AAGGTACGAT TCAGGAACTG
    GGACAGAATC AGTATGCAAT GAGTGGTGAA ATATATGTGC TGGACCGGAA CACAATTGAA
    ATCACAGAAC TTCCTGTCAG GACATGGACC CAGGTTTACA AAGAACAAGT GCTGGAGCCC
    ATGCTCAATG GAACCGAGAA AACACCATCG TTAATCTCTG ATTACAAAGA GTATCATACA
    GACACAACTG TAAAATTTGT TGTCAAAATG ACAGAAGAAA AGTTGGCACA GGCAGAAGCT
    GCTGGCTTGC ATAAAGTCTT TAAACTTCAA ACAAGTCTTA CTTGCAATTC TATGGTATTA
    TTTGACCACA TGGGCTGTCT AAAGAAATAT GAAACCGTGC AGGACATTCT GAAGGAATTT
    TTTGATCTCC GGTTAAATTA CTACACCCTG CGCAAAGAAT GGCTGATAGG AATGTTGGGA
    GCTGAAGCTG CAAGACTGAA CAACCAAGCC CGGTTTGTCC TGGAGAAGAT ACAAGGCAAA
    ATCTCTATAG AGAACAAAAC CAAAAGAGAT CTAATTCAGA TGTTGGTGCA GAAAGGATTT
    GAGTCAGACC CTGTGAAAGC TTGGAAGGAG GCACAAGAAA AAGCAACAGA GGAGGATGAC
    CTTCAAGATC CGAATGAAGA CAATTCATCC GACTCTGGCA CTGGATCAGG ACCGGATTTT
    AACTACATTC TCAATATGTC TCTCTGGAGC CTTTCAAAGG AAAAAGTAGA TGAGCTTGTC
    AGACAGAGGG ATATAAAGAG CAAAGAACTG AATGACCTCA AGAGGAAGTC TCCGCCTGAT
    CTGTGGAAGG AAGACTTGGC AGCTTTTGTT GAAGAGCTAG ACAGAGTGGA AGCTCAAGAA
    AAAGAAGATG TCTTAGCAGG GTTTGCAGGC AAAGCAATAA AAGGCAAAAT TGGAAAACCC
    AAAATGAAGA AATTACAGCT TGAGGAGACT TTGCCATCTC TTTACGGCAG AAGAGTAGAG
    CCACAAATCA GTGCAATGAA AGCAGATGCC AGCAAAAAGA TGCTAAAGAA AAAGAAGGGT
    GAAATTGACT CTATTGCACT GAAAATGGAA TTTGATGAGG AGTTTAGCTC GGCACCAGCA
    GAGGGAGGAG CTGAAGATTC CATGAATACC ACTCCTGTAA AACCTGTAAA GCCCAAGAGA
    GAAAGAAAAG AACCAGGTCC TCGAGCCAAA AAGACTGTAT TAACACCCAA GTCATCAACC
    AAGAAAGGGA AGAAGCGAAA CCCTTGGTCA GACAATGAGT CAAAATCAGA CAGTGACCTG
    GAAGAAGCTG AGGCAGAGCC TGTGGTCATT CCTCGGGACT CTCTCCTAAG GAGAGCAGCT
    GCTGAAAGAC CGAAGTACAC GTTTGATTTC TCTGAAGAGG AAGATGCAGA CGATGACGAC
    GAAGGTGTCA TTAGCCGTAG TAATAATAAT AATAATATTA ATGACTTAAA ACCGCCAGCT
    TCTCCTGCTG CTAATAGCCG AGGTGAAGAT GATGATGTTC TCTCTGACAG CCAAGAAAAG
    GATGACTATG AATTCTCCCC TTTCAAGTCT ACCCCAGCCG CACAAAAGCC TTCAGAAACA
    CAAAAGAAAA AAGACTACAT ATTTTCATCA TCTTCATTTT CAGAAAAAGT AGAAGATTCT
    GCAATATATA ACAGTGACGA TGATGAACCC TCCCCACTTT ACTCTTCGTA CTCCTTCAAA
    ACTTCAGAGA AAACAACAGG CAAAGGATCT AGTAAAAAAG AATCTGCAAA ATATGACAGT
    GATGATGATG ATGATGATGA TGAACCCTCC CCCCTTTACT CCTCATATTC CTTCAAATCT
    ACAGAAAAGC CAGCCAGCAA AGGATCTAGT GAGAAAGAAT CCACCAAATA TAACAGTGAC
    GATGAGGATG ATGACGATGA CGATGTACCG TTTTCGTCGT TTACCTTTAA ATCTTCCGAA
    AAGCCAGCCA GTAAAGGTTC TGCTAAGAAA ACAAAATCTT TGTCAGATGG TACTCCTAAA
    CCAAAACGAC CGCCAAAACC AAAGAAGTCT GAGACTTCAT TGATTTCCGA CTCTGACTTG
    TTAGATTTTG GTGCATCAAA GAAATCTGCA ACACCCAAAG CAAAAGGAAG GGGAAGGAAA
    CGAAAAACAT CTGATTCTGA AAATGAAGGA GATTACAATC CAGGGCGAAA ACCTACAAAA
    CCTGCATCTT CTAGCAAAAA ATCCAAGAAA GCATCATTTG AGCAGGATTC AGATGGTGAT
    CTCTTCCCTT CAGAAGTCGG CTCTGAGACC GCATCGAGGC AAAGGCCTGG CCGGGCTAGG
    AAAGAAGTCA AATATTTTGC AGACTCTGAC GATGAAGATG ATTTTGCTAT GTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.