top2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	top2
Entrez Gene ID	2540541
Full Name	DNA topoisomerase II
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021708.2	NP_595805.1	DNA topoisomerase II

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rrp2 possesses SUMO-binding ability and prevents excessive Top2 degradation by competing against the SUMO-targeted ubiquitin ligase (STUbL) for SUMO chain binding and by displacing SUMOylated Top2 from DNA.
Title: SUMO-Targeted DNA Translocase Rrp2 Protects the Genome from Top2-Induced DNA Damage.

DNA topoisomerase 2 mutant allele mildly delays the mitotic progression and activates the checkpoint protein kinase Chk1 in fission yeast Schizosaccharomyces pombe.
Title: DNA topoisomerase 2 mutant allele mildly delays the mitotic progression and activates the checkpoint protein kinase Chk1 in fission yeast Schizosaccharomyces pombe.

topoisomerase II has a role in disentangling dysfunctional telomeres
Title: A non-canonical function of topoisomerase II in disentangling dysfunctional telomeres.

Schizosaccharomyces pombe Rad32(Mre11) nuclease activity is involved in the removal of both Top2 from 5' DNA ends as well as Top1 from 3' ends in vivo and a ctp1(CtIP) deletion is defective for Top2 removal but overproficient for Top1 removal.
Title: Distinct requirements for the Rad32(Mre11) nuclease and Ctp1(CtIP) in the removal of covalently bound topoisomerase I and II from DNA.

The following top2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the top2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100712	NM_001021708.2 Latest version!	Schizosaccharomyces pombe DNA topoisomerase II (top2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

top2 ( NM_001021708.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595805.1 Schizosaccharomyces pombe DNA topoisomerase II (top2), mRNA.

Price & Availability↑

CloneID	OSc100712
Clone ID Related Accession (Same CDS sequence)	NM_001021708.2
Accession Version	NM_001021708.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DNA topoisomerase II
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021708.1.

ATGTCCATTG ATGCGGATTT CTCCGACTAT GAAGATGAAG CGTCTGGAGA CGAAAACGTT 
TTACCTAACA CAACCACCAA AAGGAAAGCA TCTACAACAT CCTCAAAGTC TCGCGCCAAG 
AAGGCAAGCA CTCCTGACTT AAGGCAAACT AGCTTGACAT CCATGACAGC TTCTGAACAG 
ATACCACTAG TTACCAACAA CGGAAATGGA AATTCTAATG TATCTACACA GTACCAGCGT 
CTTACACCGA GAGAGCATGT GCTAAGACGT CCGGATACAT ACATTGGCAG TATTGAGCCA 
ACGACTTCTG AAATGTGGGT CTTTGACTCT GAGAAGAACA AGCTGGATTA CAAAGCAGTG 
ACCTATGTTC CAGGCCTTTA CAAGATATTT GACGAAATAA TTGTCAATGC TGCTGACAAC 
AAAGTTCGCG ACCCAAACAT GAATACCCTA AAGGTGACTT TGGATCCTGA AGCAAATGTC 
ATATCAATTT ATAACAATGG CAAGGGCATT CCTATAGAGA TTCATGATAA AGAGAAAATT 
TACATCCCCG AGCTTATTTT TGGTAACTTG CTTACTTCAA GCAATTACGA CGATAATCAA 
AAGAAGGTAA CAGGAGGAAG AAATGGTTAC GGCGCAAAAC TTTGCAATAT TTTTTCTACT 
GAATTTGTCG TTGAGACAGC TGATAAGGAA AGGATGAAGA AATATAAACA AACTTGGTAT 
GACAATATGT CCAGAAAGTC TGAGCCAGTG ATCACTTCTC TCAAGAAACC CGATGAATAC 
ACAAAAATTA CATTCAAACC CGATTTAGCA AAATTTGGTA TGGACAAGAT TGATGATGAT 
ATGGTATCTA TTATAAAACG CCGTATTTAT GATATGGCAG GTACCGTTCG TGAAACTAAA 
GTTTACCTGA ACAATGAACG CATCAGTATA AGTGGATTTA AGAAATATGT GGAGATGTAT 
CTTGCTTCTG ACACTAAACC TGATGAAGAG CCTCCTAGGG TAATTTATGA ACATGTTAAT 
GACCGTTGGG ACGTGGCCTT TGCTGTTTCA GATGGACAGT TTAAGCAGGT TTCGTTTGTT 
AACAATATTT CTACCATTCG TGGAGGTACA CATGTTAATT ATGTTGCCAA TAAAATTGTC 
GATGCTATAG ATGAAGTCGT CAAGAAGGAG AATAAAAAGG CACCCGTTAA AGCGTTTCAA 
ATAAAAAATT ACGTACAGGT ATTTGTCAAT TGTCAAATAG AGAACCCATC CTTTGATTCC 
CAAACAAAGG AGACTCTCAC CACGAAAGTT TCTGCTTTTG GCTCTCAATG CACATTAAGT 
GATAAATTTT TAAAAGCTAT CAAAAAATCT TCTGTAGTGG AGGAGGTTTT AAAATTTGCT 
ACCGCTAAGG CAGATCAGCA ACTCAGTAAG GGCGATGGGG GTTTGCGTTC TCGTATAACG 
GGTTTAACCA AACTCGAAGA CGCAAATAAA GCGGGTACCA AAGAATCCCA TAAGTGTGTC 
CTTATCCTCA CTGAGGGAGA TTCTGCTAAA TCTTTAGCTG TTTCGGGTCT CAGTGTTGTT 
GGTAGAGACT ATTATGGAGT TTTTCCGTTA AGAGGAAAAC TACTGAATGT ACGGGAGGCT 
TCTCATTCCC AAATCCTAAA TAACAAAGAA ATTCAAGCTA TCAAGAAAAT CATGGGATTC 
ACTCATAAAA AAACTTATAC CGATGTGAAA GGTCTTCGTT ATGGTCATTT AATGATTATG 
ACAGATCAAG ATCATGATGG ATCACATATT AAAGGTTTAA TTATTAATTA TTTAGAATCC 
TCGTATCCTT CTCTTTTGCA AATTCCTGGA TTCTTGATCC AATTTATCAC ACCTATCATT 
AAGTGCACTC GTGGCAATCA GGTTCAAGCA TTTTACACTT TACCCGAGTA CGAATACTGG 
AAGGAAGCTA ATAACAATGG ACGTGGTTGG AAAATTAAAT ACTACAAGGG TTTGGGAACA 
AGTGACCATG ACGATATGAA AAGTTATTTT TCAGACCTTG ATCGTCACAT GAAGTACTTT 
CATGCCATGC AGGAGAAGGA TGCTGAATTA ATTGAAATGG CATTTGCCAA GAAAAAGGCC 
GATGTGCGTA AGGAATGGCT TAGAACGTAT CGACCTGGTA TATATATGGA TTATACTCAA 
CCTCAGATTC CGATTGATGA CTTCATTAAT CGAGAGCTTA TCCAATTCAG TATGGCTGAT 
AATATCCGTT CGATTCCTTC AGTAGTAGAT GGCTTGAAGC CTGGTCAGCG TAAAGTTGTC 
TATTATTGTT TTAAACGCAA TCTCGTCCAT GAAACTAAAG TCAGTAGACT TGCCGGCTAT 
GTTGCTAGTG AAACTGCATA CCACCATGGC GAGGTTTCGA TGGAGCAAAC TATAGTTAAT 
CTTGCCCAAA ATTTTGTTGG CAGTAACAAC ATTAATTTGC TGATGCCTAA TGGACAATTC 
GGTACACGAT CTGAGGGTGG AAAAAATGCA TCGGCTTCAA GGTATCTAAA TACAGCTTTA 
TCACCCTTAG CACGTGTTTT ATTTAATTCC AATGATGACC AGCTTCTTAA TTACCAAAAC 
GACGAAGGCC AGTGGATTGA ACCAGAGTAT TATGTGCCAA TTCTTCCCAT GGTACTTGTC 
AATGGAGCCG AAGGTATTGG TACTGGCTGG TCTACTTTTA TTCCTAATTA TAACCCTAAG 
GATATAACTG CTAATTTAAG ACATATGCTT AATGGCGAGC CTTTGGAAAT TATGACTCCC 
TGGTACCGCG GCTTTCGTGG AAGTATAACG AAAGTTGCGC CTGACAGGTA CAAAATATCT 
GGTATAATTA ACCAAATTGG TGAAAACAAA GTAGAAATTA CTGAATTGCC TATACGATTT 
TGGACTCAGG ATATGAAGGA GTATCTGGAG GCTGGTCTTG TTGGTACGGA AAAAATTCGT 
AAATTCATCG TGGACTATGA AAGCCATCAC GGTGAAGGAA ATGTTCACTT TAATGTCACG 
CTTACCGAAG CTGGCATGAA AGAAGCATTA AATGAATCCT TGGAAGTTAA GTTTAAACTG 
TCGCGTACTC AAGCAACGAG TAATATGATT GCTTTTGATG CATCTGGACG GATCAAGAAG 
TATGACAGTG TTGAAGATAT TTTGACCGAG TTTTACGAAG TACGTTTAAG GACATACCAG 
AGACGTAAAG AGCATATGGT TAATGAATTG GAAAAAAGGT TTGATAGATT TTCAAATCAA 
GCGCGGTTTA TTCATATGAT TATAGAGGGG GAGCTTGTAG TTTCAAAAAA GAAGAAAAAG 
GATCTCATTG TGGAGCTGAA GGAAAAGAAG TTTCAACCTA TCAGTAAACC AAAGAAAGGG 
CATTTGGTTG ATTTAGAAGT TGAAAATGCT CTTGCTGAAG AAGAACAATC TGGTGATGTT 
TCGCAAGACG AGGATTCAGA TGCCTACAAT TACCTTCTTT CAATGCCTTT ATGGTCTTTG 
ACCTATGAAC GGTATGTGGA ACTTCTCAAG AAAAAGGACG AAGTAATGGC CGAACTGGAT 
GCTTTGATTA AAAAAACTCC TAAAGAATTG TGGCTTCATG ATTTGGATGC ATTTGAGCAT 
GCTTGGAATA AGGTTATGGA TGATATTCAG AGAGAAATGT TAGAAGAAGA GCAATCCTCT 
AGAGATTTTG TAAATCGAAC GAAAAAGAAG CCTCGAGGTA AATCCACTGG TACTAGGAAA 
CCCAGGGCTA TTGCCGGATC CTCCTCAAGT ACTGCTGTTA AAAAAGAAGC TAGCAGCGAA 
TCCAAACCAT CTACAACAAA TCGAAAGCAA CAAACTTTAC TTGAGTTCGC AGCGTCGAAA 
GAACCTGAGA AATCATCTGA CATAAACATT GTCAAGACAG AGGATAACTC TCATGGCTTA 
TCAGTTGAAG AAAATCGCAT ATCTAAGAGC CCGGGATTGG ATAGCAGTGA TAGTGGAAAA 
TCAAGAAAAA GAAGTCAATC TGTTGACTCT GAAGACGCAG GTAGTAAAAA ACCCGTCAAA 
AAAATAGCAG CCTCGGCGTC TGGAAGAGGA AGAAAGACTA ATAAACCAGT TGCTACTACC 
ATATTTTCTT CAGACGATGA AGATGATTTG TTGCCTAGCA GTTTGAAACC TTCCACCATA 
ACATCCACGA AGGCTTCTGC GAAGAATAAA GGGAAAAAAG CAAGCTCGGT AAAGAAACAA 
TCACCTGAAG ATGACGATGA TGATTTTATT ATTCCAGGTA GTAGTTCAAC TCCAAAAGCT 
AGTTCGACTA ACGCAGAGCC ACCAGAAGAT TCAGATTCTC CGATTAGAAA GAGGCCAACC 
AGAAGAGCAG CGGCTACAGT GAAAACACCT ATTTACGTTG ATCCCTCGTT TGATAGCATG 
GATGAACCCA GCATGCAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT 
TATGATGAGA GTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595805.1
CDS	2077..6534
Translation	MSIDADFSDYEDEASGDENVLPNTTTKRKASTTSSKSRAKKASTPDLRQTSLTSMTASEQIPLVTNNGNGNSNVSTQYQRLTPREHVLRRPDTYIGSIEPTTSEMWVFDSEKNKLDYKAVTYVPGLYKIFDEIIVNAADNKVRDPNMNTLKVTLDPEANVISIYNNGKGIPIEIHDKEKIYIPELIFGNLLTSSNYDDNQKKVTGGRNGYGAKLCNIFSTEFVVETADKERMKKYKQTWYDNMSRKSEPVITSLKKPDEYTKITFKPDLAKFGMDKIDDDMVSIIKRRIYDMAGTVRETKVYLNNERISISGFKKYVEMYLASDTKPDEEPPRVIYEHVNDRWDVAFAVSDGQFKQVSFVNNISTIRGGTHVNYVANKIVDAIDEVVKKENKKAPVKAFQIKNYVQVFVNCQIENPSFDSQTKETLTTKVSAFGSQCTLSDKFLKAIKKSSVVEEVLKFATAKADQQLSKGDGGLRSRITGLTKLEDANKAGTKESHKCVLILTEGDSAKSLAVSGLSVVGRDYYGVFPLRGKLLNVREASHSQILNNKEIQAIKKIMGFTHKKTYTDVKGLRYGHLMIMTDQDHDGSHIKGLIINYLESSYPSLLQIPGFLIQFITPIIKCTRGNQVQAFYTLPEYEYWKEANNNGRGWKIKYYKGLGTSDHDDMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEWLRTYRPGIYMDYTQPQIPIDDFINRELIQFSMADNIRSIPSVVDGLKPGQRKVVYYCFKRNLVHETKVSRLAGYVASETAYHHGEVSMEQTIVNLAQNFVGSNNINLLMPNGQFGTRSEGGKNASASRYLNTALSPLARVLFNSNDDQLLNYQNDEGQWIEPEYYVPILPMVLVNGAEGIGTGWSTFIPNYNPKDITANLRHMLNGEPLEIMTPWYRGFRGSITKVAPDRYKISGIINQIGENKVEITELPIRFWTQDMKEYLEAGLVGTEKIRKFIVDYESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSVEDILTEFYEVRLRTYQRRKEHMVNELEKRFDRFSNQARFIHMIIEGELVVSKKKKKDLIVELKEKKFQPISKPKKGHLVDLEVENALAEEEQSGDVSQDEDSDAYNYLLSMPLWSLTYERYVELLKKKDEVMAELDALIKKTPKELWLHDLDAFEHAWNKVMDDIQREMLEEEQSSRDFVNRTKKKPRGKSTGTRKPRAIAGSSSSTAVKKEASSESKPSTTNRKQQTLLEFAASKEPEKSSDINIVKTEDNSHGLSVEENRISKSPGLDSSDSGKSRKRSQSVDSEDAGSKKPVKKIAASASGRGRKTNKPVATTIFSSDDEDDLLPSSLKPSTITSTKASAKNKGKKASSVKKQSPEDDDDDFIIPGSSSTPKASSTNAEPPEDSDSPIRKRPTRRAAATVKTPIYVDPSFDSMDEPSMQDDSFIVDNDEDVDDYDESD

Target ORF information:

RefSeq Version	NM_001021708.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DNA topoisomerase II (top2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021708.2

ORF Insert Sequence:

ATGTCCATTG ATGCGGATTT CTCCGACTAT GAAGATGAAG CGTCTGGAGA CGAAAACGTT 
TTACCTAACA CAACCACCAA AAGGAAAGCA TCTACAACAT CCTCAAAGTC TCGCGCCAAG 
AAGGCAAGCA CTCCTGACTT AAGGCAAACT AGCTTGACAT CCATGACAGC TTCTGAACAG 
ATACCACTAG TTACCAACAA CGGAAATGGA AATTCTAATG TATCTACACA GTACCAGCGT 
CTTACACCGA GAGAGCATGT GCTAAGACGT CCGGATACAT ACATTGGCAG TATTGAGCCA 
ACGACTTCTG AAATGTGGGT CTTTGACTCT GAGAAGAACA AGCTGGATTA CAAAGCAGTG 
ACCTATGTTC CAGGCCTTTA CAAGATATTT GACGAAATAA TTGTCAATGC TGCTGACAAC 
AAAGTTCGCG ACCCAAACAT GAATACCCTA AAGGTGACTT TGGATCCTGA AGCAAATGTC 
ATATCAATTT ATAACAATGG CAAGGGCATT CCTATAGAGA TTCATGATAA AGAGAAAATT 
TACATCCCCG AGCTTATTTT TGGTAACTTG CTTACTTCAA GCAATTACGA CGATAATCAA 
AAGAAGGTAA CAGGAGGAAG AAATGGTTAC GGCGCAAAAC TTTGCAATAT TTTTTCTACT 
GAATTTGTCG TTGAGACAGC TGATAAGGAA AGGATGAAGA AATATAAACA AACTTGGTAT 
GACAATATGT CCAGAAAGTC TGAGCCAGTG ATCACTTCTC TCAAGAAACC CGATGAATAC 
ACAAAAATTA CATTCAAACC CGATTTAGCA AAATTTGGTA TGGACAAGAT TGATGATGAT 
ATGGTATCTA TTATAAAACG CCGTATTTAT GATATGGCAG GTACCGTTCG TGAAACTAAA 
GTTTACCTGA ACAATGAACG CATCAGTATA AGTGGATTTA AGAAATATGT GGAGATGTAT 
CTTGCTTCTG ACACTAAACC TGATGAAGAG CCTCCTAGGG TAATTTATGA ACATGTTAAT 
GACCGTTGGG ACGTGGCCTT TGCTGTTTCA GATGGACAGT TTAAGCAGGT TTCGTTTGTT 
AACAATATTT CTACCATTCG TGGAGGTACA CATGTTAATT ATGTTGCCAA TAAAATTGTC 
GATGCTATAG ATGAAGTCGT CAAGAAGGAG AATAAAAAGG CACCCGTTAA AGCGTTTCAA 
ATAAAAAATT ACGTACAGGT ATTTGTCAAT TGTCAAATAG AGAACCCATC CTTTGATTCC 
CAAACAAAGG AGACTCTCAC CACGAAAGTT TCTGCTTTTG GCTCTCAATG CACATTAAGT 
GATAAATTTT TAAAAGCTAT CAAAAAATCT TCTGTAGTGG AGGAGGTTTT AAAATTTGCT 
ACCGCTAAGG CAGATCAGCA ACTCAGTAAG GGCGATGGGG GTTTGCGTTC TCGTATAACG 
GGTTTAACCA AACTCGAAGA CGCAAATAAA GCGGGTACCA AAGAATCCCA TAAGTGTGTC 
CTTATCCTCA CTGAGGGAGA TTCTGCTAAA TCTTTAGCTG TTTCGGGTCT CAGTGTTGTT 
GGTAGAGACT ATTATGGAGT TTTTCCGTTA AGAGGAAAAC TACTGAATGT ACGGGAGGCT 
TCTCATTCCC AAATCCTAAA TAACAAAGAA ATTCAAGCTA TCAAGAAAAT CATGGGATTC 
ACTCATAAAA AAACTTATAC CGATGTGAAA GGTCTTCGTT ATGGTCATTT AATGATTATG 
ACAGATCAAG ATCATGATGG ATCACATATT AAAGGTTTAA TTATTAATTA TTTAGAATCC 
TCGTATCCTT CTCTTTTGCA AATTCCTGGA TTCTTGATCC AATTTATCAC ACCTATCATT 
AAGTGCACTC GTGGCAATCA GGTTCAAGCA TTTTACACTT TACCCGAGTA CGAATACTGG 
AAGGAAGCTA ATAACAATGG ACGTGGTTGG AAAATTAAAT ACTACAAGGG TTTGGGAACA 
AGTGACCATG ACGATATGAA AAGTTATTTT TCAGACCTTG ATCGTCACAT GAAGTACTTT 
CATGCCATGC AGGAGAAGGA TGCTGAATTA ATTGAAATGG CATTTGCCAA GAAAAAGGCC 
GATGTGCGTA AGGAATGGCT TAGAACGTAT CGACCTGGTA TATATATGGA TTATACTCAA 
CCTCAGATTC CGATTGATGA CTTCATTAAT CGAGAGCTTA TCCAATTCAG TATGGCTGAT 
AATATCCGTT CGATTCCTTC AGTAGTAGAT GGCTTGAAGC CTGGTCAGCG TAAAGTTGTC 
TATTATTGTT TTAAACGCAA TCTCGTCCAT GAAACTAAAG TCAGTAGACT TGCCGGCTAT 
GTTGCTAGTG AAACTGCATA CCACCATGGC GAGGTTTCGA TGGAGCAAAC TATAGTTAAT 
CTTGCCCAAA ATTTTGTTGG CAGTAACAAC ATTAATTTGC TGATGCCTAA TGGACAATTC 
GGTACACGAT CTGAGGGTGG AAAAAATGCA TCGGCTTCAA GGTATCTAAA TACAGCTTTA 
TCACCCTTAG CACGTGTTTT ATTTAATTCC AATGATGACC AGCTTCTTAA TTACCAAAAC 
GACGAAGGCC AGTGGATTGA ACCAGAGTAT TATGTGCCAA TTCTTCCCAT GGTACTTGTC 
AATGGAGCCG AAGGTATTGG TACTGGCTGG TCTACTTTTA TTCCTAATTA TAACCCTAAG 
GATATAACTG CTAATTTAAG ACATATGCTT AATGGCGAGC CTTTGGAAAT TATGACTCCC 
TGGTACCGCG GCTTTCGTGG AAGTATAACG AAAGTTGCGC CTGACAGGTA CAAAATATCT 
GGTATAATTA ACCAAATTGG TGAAAACAAA GTAGAAATTA CTGAATTGCC TATACGATTT 
TGGACTCAGG ATATGAAGGA GTATCTGGAG GCTGGTCTTG TTGGTACGGA AAAAATTCGT 
AAATTCATCG TGGACTATGA AAGCCATCAC GGTGAAGGAA ATGTTCACTT TAATGTCACG 
CTTACCGAAG CTGGCATGAA AGAAGCATTA AATGAATCCT TGGAAGTTAA GTTTAAACTG 
TCGCGTACTC AAGCAACGAG TAATATGATT GCTTTTGATG CATCTGGACG GATCAAGAAG 
TATGACAGTG TTGAAGATAT TTTGACCGAG TTTTACGAAG TACGTTTAAG GACATACCAG 
AGACGTAAAG AGCATATGGT TAATGAATTG GAAAAAAGGT TTGATAGATT TTCAAATCAA 
GCGCGGTTTA TTCATATGAT TATAGAGGGG GAGCTTGTAG TTTCAAAAAA GAAGAAAAAG 
GATCTCATTG TGGAGCTGAA GGAAAAGAAG TTTCAACCTA TCAGTAAACC AAAGAAAGGG 
CATTTGGTTG ATTTAGAAGT TGAAAATGCT CTTGCTGAAG AAGAACAATC TGGTGATGTT 
TCGCAAGACG AGGATTCAGA TGCCTACAAT TACCTTCTTT CAATGCCTTT ATGGTCTTTG 
ACCTATGAAC GGTATGTGGA ACTTCTCAAG AAAAAGGACG AAGTAATGGC CGAACTGGAT 
GCTTTGATTA AAAAAACTCC TAAAGAATTG TGGCTTCATG ATTTGGATGC ATTTGAGCAT 
GCTTGGAATA AGGTTATGGA TGATATTCAG AGAGAAATGT TAGAAGAAGA GCAATCCTCT 
AGAGATTTTG TAAATCGAAC GAAAAAGAAG CCTCGAGGTA AATCCACTGG TACTAGGAAA 
CCCAGGGCTA TTGCCGGATC CTCCTCAAGT ACTGCTGTTA AAAAAGAAGC TAGCAGCGAA 
TCCAAACCAT CTACAACAAA TCGAAAGCAA CAAACTTTAC TTGAGTTCGC AGCGTCGAAA 
GAACCTGAGA AATCATCTGA CATAAACATT GTCAAGACAG AGGATAACTC TCATGGCTTA 
TCAGTTGAAG AAAATCGCAT ATCTAAGAGC CCGGGATTGG ATAGCAGTGA TAGTGGAAAA 
TCAAGAAAAA GAAGTCAATC TGTTGACTCT GAAGACGCAG GTAGTAAAAA ACCCGTCAAA 
AAAATAGCAG CCTCGGCGTC TGGAAGAGGA AGAAAGACTA ATAAACCAGT TGCTACTACC 
ATATTTTCTT CAGACGATGA AGATGATTTG TTGCCTAGCA GTTTGAAACC TTCCACCATA 
ACATCCACGA AGGCTTCTGC GAAGAATAAA GGGAAAAAAG CAAGCTCGGT AAAGAAACAA 
TCACCTGAAG ATGACGATGA TGATTTTATT ATTCCAGGTA GTAGTTCAAC TCCAAAAGCT 
AGTTCGACTA ACGCAGAGCC ACCAGAAGAT TCAGATTCTC CGATTAGAAA GAGGCCAACC 
AGAAGAGCAG CGGCTACAGT GAAAACACCT ATTTACGTTG ATCCCTCGTT TGATAGCATG 
GATGAACCCA GCATGCAAGA TGATTCTTTT ATCGTCGATA ACGATGAGGA TGTAGACGAT 
TATGATGAGA GTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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top2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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