TOP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2
Entrez Gene ID	855636
Full Name	DNA topoisomerase 2
Synonyms	TOR3,TRF3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182926.3	NP_014311.3	DNA topoisomerase 2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Condensin DNA supercoiling activity correlates with Top2 intertwine removal during DNA replication.
Title: Physical Proximity of Sister Chromatids Promotes Top2-Dependent Intertwining.

Our data are consistent with a redundant role of Top2 and Sgs1 together with Top3 (Sgs1-Top3) in replication fork merging at rDNA barriers.
Title: Top2 and Sgs1-Top3 Act Redundantly to Ensure rDNA Replication Termination.

Topoisomerase II plays a central role in a molecular pathway for crossover interference
Title: Topoisomerase II mediates meiotic crossover interference.

Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils.
Title: Chromatin regulates DNA torsional energy via topoisomerase II-mediated relaxation of positive supercoils.

the cell cycle delay depends on the divergent and catalytically inert C-terminal region of Topo II, indicating that transmission of a checkpoint signal may occur via the C-terminus
Title: Direct monitoring of the strand passage reaction of DNA topoisomerase II triggers checkpoint activation.

The following TOP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04543	NM_001182926.3 Latest version!	Saccharomyces cerevisiae S288C DNA topoisomerase 2 (TOP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2 ( NM_001182926.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014311.3 Saccharomyces cerevisiae S288C DNA topoisomerase 2 (TOP2), partial mRNA.

Price & Availability↑

CloneID	OSi04543
Clone ID Related Accession (Same CDS sequence)	NM_001182926.3
Accession Version	NM_001182926.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	DNA topoisomerase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). On Jul 30, 2012 this sequence version replaced NM_001182926.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCAACTG AACCGGTAAG CGCCTCTGAT AAATATCAGA AAATTTCTCA ACTGGAACAT 
ATCTTAAAAA GACCAGACAC TTATATCGGT TCTGTTGAAA CTCAAGAGCA GCTGCAATGG 
ATATACGATG AAGAGACCGA TTGCATGATT GAAAAAAATG TCACAATTGT ACCAGGGTTG 
TTCAAAATCT TTGATGAAAT CTTAGTCAAT GCGGCAGATA ATAAAGTTCG TGATCCATCG 
ATGAAACGAA TCGATGTAAA CATACATGCT GAGGAACATA CTATAGAAGT GAAAAATGAT 
GGAAAAGGTA TTCCCATAGA GATTCATAAC AAGGAGAATA TTTATATTCC TGAAATGATA 
TTTGGTCATT TGTTGACATC ATCCAATTAT GATGATGATG AGAAGAAAGT CACTGGTGGT 
AGAAACGGTT ATGGTGCTAA GCTTTGTAAT ATATTTTCCA CTGAATTCAT ATTAGAAACT 
GCAGATCTAA ATGTTGGCCA GAAATATGTT CAAAAATGGG AAAATAACAT GAGCATTTGC 
CACCCCCCAA AAATAACATC TTACAAGAAG GGTCCATCAT ATACAAAGGT GACATTTAAG 
CCGGATTTAA CCAGATTCGG AATGAAAGAG CTAGATAATG ATATCTTAGG AGTGATGCGA 
AGAAGAGTTT ATGATATCAA TGGTTCTGTT CGTGACATTA ATGTCTATCT GAATGGCAAG 
TCCTTAAAGA TAAGAAATTT CAAAAATTAT GTTGAACTCT ACTTGAAATC ACTCGAAAAA 
AAAAGACAAC TAGATAACGG TGAGGACGGT GCCGCTAAGT CTGATATCCC GACTATTCTT 
TATGAGAGAA TAAACAACAG ATGGGAAGTT GCTTTTGCGG TTTCTGATAT CTCTTTTCAA 
CAAATTTCTT TTGTGAATTC CATTGCAACT ACCATGGGTG GTACCCATGT CAATTACATA 
ACAGACCAAA TTGTAAAAAA AATTTCAGAA ATTTTGAAGA AAAAGAAGAA AAAAAGTGTG 
AAGTCTTTTC AGATTAAAAA TAATATGTTC ATTTTCATTA ATTGTTTGAT TGAGAATCCT 
GCATTTACCT CACAAACAAA AGAGCAACTG ACAACAAGAG TCAAAGATTT TGGGTCCCGT 
TGTGAGATTC CTCTTGAATA TATTAATAAG ATTATGAAAA CTGATTTGGC TACAAGAATG 
TTTGAAATTG CCGACGCAAA TGAAGAAAAT GCGCTAAAGA AGTCTGATGG TACAAGGAAA 
AGCAGAATTA CTAATTACCC TAAACTGGAA GATGCCAACA AAGCCGGTAC AAAAGAAGGC 
TATAAATGTA CTTTAGTTCT GACAGAAGGG GATTCCGCCT TGTCATTAGC TGTTGCAGGT 
TTAGCTGTTG TTGGTAGAGA TTATTATGGT TGTTATCCAC TTCGTGGTAA AATGCTGAAT 
GTTAGAGAAG CTAGTGCTGA TCAGATACTA AAAAACGCGG AAATTCAAGC CATTAAAAAA 
ATTATGGGGT TACAACATCG CAAGAAATAT GAAGATACAA AATCTTTAAG ATATGGGCAT 
CTTATGATCA TGACCGATCA AGATCATGAT GGTTCGCATA TTAAAGGTTT AATTATAAAC 
TTTTTAGAAA GCTCATTTCC TGGTCTTTTG GATATCCAAG GTTTCTTACT TGAATTCATA 
ACTCCGATCA TCAAAGTTTC CATCACTAAA CCAACAAAAA ACACTATTGC ATTCTACAAT 
ATGCCGGACT ATGAAAAATG GAGAGAGGAA GAATCGCACA AATTTACTTG GAAGCAGAAG 
TATTATAAAG GATTAGGGAC TTCTCTAGCA CAAGAAGTCC GAGAATATTT TTCGAACTTG 
GACAGACATT TGAAAATATT CCATTCTTTG CAGGGTAATG ATAAAGATTA CATTGATTTA 
GCTTTCTCCA AGAAAAAGGC AGATGACCGT AAAGAATGGC TGAGACAATA CGAACCTGGT 
ACTGTTTTAG ACCCTACTTT AAAAGAGATT CCAATTAGCG ACTTCATTAA TAAGGAATTA 
ATCCTTTTTT CTTTGGCCGA TAATATACGG TCGATTCCCA ATGTTTTAGA TGGATTTAAA 
CCTGGCCAAA GAAAAGTTCT TTATGGTTGT TTCAAAAAAA ATTTAAAGTC GGAACTGAAA 
GTAGCTCAAC TTGCACCATA CGTGAGCGAA TGTACGGCAT ATCACCATGG TGAGCAGTCA 
TTGGCACAAA CTATTATTGG GCTAGCCCAA AACTTTGTTG GGTCCAACAA TATTTACTTG 
CTATTACCTA ACGGTGCTTT CGGTACAAGA GCCACTGGTG GTAAAGATGC AGCGGCAGCG 
AGATATATCT ACACAGAATT GAACAAATTA ACTCGTAAGA TATTTCACCC TGCTGATGAT 
CCATTATACA AATATATACA AGAAGATGAG AAAACAGTGG AGCCAGAGTG GTATTTACCA 
ATTCTTCCTA TGATTCTTGT TAACGGTGCT GAGGGTATTG GCACTGGCTG GAGTACTTAC 
ATTCCTCCAT TCAACCCATT GGAAATTATA AAGAATATAA GACATTTAAT GAACGACGAG 
GAGCTTGAGC AAATGCATCC GTGGTTTAGG GGATGGACCG GTACTATTGA AGAAATTGAG 
CCTCTGCGTT ACAGAATGTA CGGTAGGATT GAACAAATTG GAGATAACGT CTTAGAAATA 
ACTGAGTTGC CAGCCAGAAC TTGGACATCG ACTATAAAGG AGTACTTACT TTTAGGTTTA 
AGCGGTAACG ATAAAATAAA ACCCTGGATC AAAGATATGG AGGAGCAGCA CGATGATAAC 
ATCAAATTCA TAATCACGCT ATCACCTGAG GAAATGGCTA AAACAAGGAA AATAGGTTTT 
TATGAAAGAT TTAAACTAAT TTCGCCTATA AGTTTGATGA ATATGGTCGC ATTTGATCCT 
CACGGGAAAA TCAAGAAGTA CAATTCCGTG AATGAAATAT TAAGCGAATT TTACTACGTC 
AGACTAGAAT ACTATCAAAA AAGAAAAGAC CATATGAGCG AAAGGTTACA GTGGGAGGTA 
GAGAAATACT CTTTCCAAGT AAAATTTATT AAAATGATTA TTGAAAAGGA GTTAACAGTC 
ACCAATAAGC CTAGGAACGC TATTATCCAA GAACTTGAGA ATTTAGGGTT CCCCAGATTT 
AATAAGGAAG GTAAACCATA TTATGGAAGT CCTAACGATG AGATAGCTGA ACAAATTAAC 
GACGTAAAAG GCGCAACTTC TGATGAAGAA GATGAAGAAA GTTCACACGA AGATACTGAA 
AATGTTATAA ATGGTCCTGA AGAACTATAT GGCACATATG AATATTTATT AGGAATGAGA 
ATATGGTCAT TGACCAAGGA AAGATATCAA AAGCTGTTGA AACAAAAACA AGAAAAGGAG 
ACAGAGTTGG AAAACTTGTT AAAACTTTCC GCGAAAGATA TATGGAACAC TGACTTGAAG 
GCTTTTGAGG TGGGATATCA AGAATTTTTG CAACGAGATG CAGAAGCTCG CGGTGGTAAT 
GTTCCCAATA AAGGGAGCAA AACGAAAGGT AAAGGAAAAA GAAAGCTTGT TGACGACGAA 
GACTACGACC CATCAAAAAA AAACAAGAAA AGTACTGCTA GAAAGGGCAA AAAAATTAAG 
TTAGAGGATA AGAATTTTGA AAGGATTTTG TTAGAACAAA AACTAGTAAC CAAAAGCAAG 
GCGCCTACAA AGATTAAAAA AGAGAAAACG CCTTCTGTTT CAGAAACAAA AACAGAAGAA 
GAAGAGAATG CTCCTTCTTC CACGAGTTCT TCTTCTATTT TCGACATAAA GAAAGAAGAT 
AAAGATGAGG GCGAACTGAG TAAGATTTCG AACAAGTTTA AAAAAATTAG CACGATTTTT 
GACAAAATGG GTTCTACTTC CGCTACATCG AAGGAAAATA CACCAGAACA GGACGATGTA 
GCCACTAAAA AAAATCAAAC AACCGCTAAA AAAACAGCTG TAAAACCTAA ATTGGCCAAG 
AAGCCAGTCA GGAAACAACA AAAAGTTGTG GAACTATCTG GTGAAAGCGA CCTAGAAATT 
TTAGATTCAT ACACTGATCG GGAAGATAGC AATAAAGATG AAGATGATGC TATACCACAA 
CGATCAAGGA GACAAAGATC GTCGAGAGCT GCGTCGGTTC CTAAGAAATC TTACGTTGAA 
ACTTTAGAAT TATCTGACGA CAGTTTCATC GAAGATGATG AAGAGGAAAA CCAAGGATCA 
GATGTTTCGT TCAATGAAGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014311.3
CDS	1..4287
Translation	MSTEPVSASDKYQKISQLEHILKRPDTYIGSVETQEQLQWIYDEETDCMIEKNVTIVPGLFKIFDEILVNAADNKVRDPSMKRIDVNIHAEEHTIEVKNDGKGIPIEIHNKENIYIPEMIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTEFILETADLNVGQKYVQKWENNMSICHPPKITSYKKGPSYTKVTFKPDLTRFGMKELDNDILGVMRRRVYDINGSVRDINVYLNGKSLKIRNFKNYVELYLKSLEKKRQLDNGEDGAAKSDIPTILYERINNRWEVAFAVSDISFQQISFVNSIATTMGGTHVNYITDQIVKKISEILKKKKKKSVKSFQIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIMKTDLATRMFEIADANEENALKKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKYEDTKSLRYGHLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQGFLLEFITPIIKVSITKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFHSLQGNDKDYIDLAFSKKKADDRKEWLRQYEPGTVLDPTLKEIPISDFINKELILFSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPWFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPARTWTSTIKEYLLLGLSGNDKIKPWIKDMEEQHDDNIKFIITLSPEEMAKTRKIGFYERFKLISPISLMNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYSFQVKFIKMIIEKELTVTNKPRNAIIQELENLGFPRFNKEGKPYYGSPNDEIAEQINDVKGATSDEEDEESSHEDTENVINGPEELYGTYEYLLGMRIWSLTKERYQKLLKQKQEKETELENLLKLSAKDIWNTDLKAFEVGYQEFLQRDAEARGGNVPNKGSKTKGKGKRKLVDDEDYDPSKKNKKSTARKGKKIKLEDKNFERILLEQKLVTKSKAPTKIKKEKTPSVSETKTEEEENAPSSTSSSSIFDIKKEDKDEGELSKISNKFKKISTIFDKMGSTSATSKENTPEQDDVATKKNQTTAKKTAVKPKLAKKPVRKQQKVVELSGESDLEILDSYTDREDSNKDEDDAIPQRSRRQRSSRAASVPKKSYVETLELSDDSFIEDDEEENQGSDVSFNEED

Target ORF information:

RefSeq Version	NM_001182926.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C DNA topoisomerase 2 (TOP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182926.3

ORF Insert Sequence:

ATGTCAACTG AACCGGTAAG CGCCTCTGAT AAATATCAGA AAATTTCTCA ACTGGAACAT 
ATCTTAAAAA GACCAGACAC TTATATCGGT TCTGTTGAAA CTCAAGAGCA GCTGCAATGG 
ATATACGATG AAGAGACCGA TTGCATGATT GAAAAAAATG TCACAATTGT ACCAGGGTTG 
TTCAAAATCT TTGATGAAAT CTTAGTCAAT GCGGCAGATA ATAAAGTTCG TGATCCATCG 
ATGAAACGAA TCGATGTAAA CATACATGCT GAGGAACATA CTATAGAAGT GAAAAATGAT 
GGAAAAGGTA TTCCCATAGA GATTCATAAC AAGGAGAATA TTTATATTCC TGAAATGATA 
TTTGGTCATT TGTTGACATC ATCCAATTAT GATGATGATG AGAAGAAAGT CACTGGTGGT 
AGAAACGGTT ATGGTGCTAA GCTTTGTAAT ATATTTTCCA CTGAATTCAT ATTAGAAACT 
GCAGATCTAA ATGTTGGCCA GAAATATGTT CAAAAATGGG AAAATAACAT GAGCATTTGC 
CACCCCCCAA AAATAACATC TTACAAGAAG GGTCCATCAT ATACAAAGGT GACATTTAAG 
CCGGATTTAA CCAGATTCGG AATGAAAGAG CTAGATAATG ATATCTTAGG AGTGATGCGA 
AGAAGAGTTT ATGATATCAA TGGTTCTGTT CGTGACATTA ATGTCTATCT GAATGGCAAG 
TCCTTAAAGA TAAGAAATTT CAAAAATTAT GTTGAACTCT ACTTGAAATC ACTCGAAAAA 
AAAAGACAAC TAGATAACGG TGAGGACGGT GCCGCTAAGT CTGATATCCC GACTATTCTT 
TATGAGAGAA TAAACAACAG ATGGGAAGTT GCTTTTGCGG TTTCTGATAT CTCTTTTCAA 
CAAATTTCTT TTGTGAATTC CATTGCAACT ACCATGGGTG GTACCCATGT CAATTACATA 
ACAGACCAAA TTGTAAAAAA AATTTCAGAA ATTTTGAAGA AAAAGAAGAA AAAAAGTGTG 
AAGTCTTTTC AGATTAAAAA TAATATGTTC ATTTTCATTA ATTGTTTGAT TGAGAATCCT 
GCATTTACCT CACAAACAAA AGAGCAACTG ACAACAAGAG TCAAAGATTT TGGGTCCCGT 
TGTGAGATTC CTCTTGAATA TATTAATAAG ATTATGAAAA CTGATTTGGC TACAAGAATG 
TTTGAAATTG CCGACGCAAA TGAAGAAAAT GCGCTAAAGA AGTCTGATGG TACAAGGAAA 
AGCAGAATTA CTAATTACCC TAAACTGGAA GATGCCAACA AAGCCGGTAC AAAAGAAGGC 
TATAAATGTA CTTTAGTTCT GACAGAAGGG GATTCCGCCT TGTCATTAGC TGTTGCAGGT 
TTAGCTGTTG TTGGTAGAGA TTATTATGGT TGTTATCCAC TTCGTGGTAA AATGCTGAAT 
GTTAGAGAAG CTAGTGCTGA TCAGATACTA AAAAACGCGG AAATTCAAGC CATTAAAAAA 
ATTATGGGGT TACAACATCG CAAGAAATAT GAAGATACAA AATCTTTAAG ATATGGGCAT 
CTTATGATCA TGACCGATCA AGATCATGAT GGTTCGCATA TTAAAGGTTT AATTATAAAC 
TTTTTAGAAA GCTCATTTCC TGGTCTTTTG GATATCCAAG GTTTCTTACT TGAATTCATA 
ACTCCGATCA TCAAAGTTTC CATCACTAAA CCAACAAAAA ACACTATTGC ATTCTACAAT 
ATGCCGGACT ATGAAAAATG GAGAGAGGAA GAATCGCACA AATTTACTTG GAAGCAGAAG 
TATTATAAAG GATTAGGGAC TTCTCTAGCA CAAGAAGTCC GAGAATATTT TTCGAACTTG 
GACAGACATT TGAAAATATT CCATTCTTTG CAGGGTAATG ATAAAGATTA CATTGATTTA 
GCTTTCTCCA AGAAAAAGGC AGATGACCGT AAAGAATGGC TGAGACAATA CGAACCTGGT 
ACTGTTTTAG ACCCTACTTT AAAAGAGATT CCAATTAGCG ACTTCATTAA TAAGGAATTA 
ATCCTTTTTT CTTTGGCCGA TAATATACGG TCGATTCCCA ATGTTTTAGA TGGATTTAAA 
CCTGGCCAAA GAAAAGTTCT TTATGGTTGT TTCAAAAAAA ATTTAAAGTC GGAACTGAAA 
GTAGCTCAAC TTGCACCATA CGTGAGCGAA TGTACGGCAT ATCACCATGG TGAGCAGTCA 
TTGGCACAAA CTATTATTGG GCTAGCCCAA AACTTTGTTG GGTCCAACAA TATTTACTTG 
CTATTACCTA ACGGTGCTTT CGGTACAAGA GCCACTGGTG GTAAAGATGC AGCGGCAGCG 
AGATATATCT ACACAGAATT GAACAAATTA ACTCGTAAGA TATTTCACCC TGCTGATGAT 
CCATTATACA AATATATACA AGAAGATGAG AAAACAGTGG AGCCAGAGTG GTATTTACCA 
ATTCTTCCTA TGATTCTTGT TAACGGTGCT GAGGGTATTG GCACTGGCTG GAGTACTTAC 
ATTCCTCCAT TCAACCCATT GGAAATTATA AAGAATATAA GACATTTAAT GAACGACGAG 
GAGCTTGAGC AAATGCATCC GTGGTTTAGG GGATGGACCG GTACTATTGA AGAAATTGAG 
CCTCTGCGTT ACAGAATGTA CGGTAGGATT GAACAAATTG GAGATAACGT CTTAGAAATA 
ACTGAGTTGC CAGCCAGAAC TTGGACATCG ACTATAAAGG AGTACTTACT TTTAGGTTTA 
AGCGGTAACG ATAAAATAAA ACCCTGGATC AAAGATATGG AGGAGCAGCA CGATGATAAC 
ATCAAATTCA TAATCACGCT ATCACCTGAG GAAATGGCTA AAACAAGGAA AATAGGTTTT 
TATGAAAGAT TTAAACTAAT TTCGCCTATA AGTTTGATGA ATATGGTCGC ATTTGATCCT 
CACGGGAAAA TCAAGAAGTA CAATTCCGTG AATGAAATAT TAAGCGAATT TTACTACGTC 
AGACTAGAAT ACTATCAAAA AAGAAAAGAC CATATGAGCG AAAGGTTACA GTGGGAGGTA 
GAGAAATACT CTTTCCAAGT AAAATTTATT AAAATGATTA TTGAAAAGGA GTTAACAGTC 
ACCAATAAGC CTAGGAACGC TATTATCCAA GAACTTGAGA ATTTAGGGTT CCCCAGATTT 
AATAAGGAAG GTAAACCATA TTATGGAAGT CCTAACGATG AGATAGCTGA ACAAATTAAC 
GACGTAAAAG GCGCAACTTC TGATGAAGAA GATGAAGAAA GTTCACACGA AGATACTGAA 
AATGTTATAA ATGGTCCTGA AGAACTATAT GGCACATATG AATATTTATT AGGAATGAGA 
ATATGGTCAT TGACCAAGGA AAGATATCAA AAGCTGTTGA AACAAAAACA AGAAAAGGAG 
ACAGAGTTGG AAAACTTGTT AAAACTTTCC GCGAAAGATA TATGGAACAC TGACTTGAAG 
GCTTTTGAGG TGGGATATCA AGAATTTTTG CAACGAGATG CAGAAGCTCG CGGTGGTAAT 
GTTCCCAATA AAGGGAGCAA AACGAAAGGT AAAGGAAAAA GAAAGCTTGT TGACGACGAA 
GACTACGACC CATCAAAAAA AAACAAGAAA AGTACTGCTA GAAAGGGCAA AAAAATTAAG 
TTAGAGGATA AGAATTTTGA AAGGATTTTG TTAGAACAAA AACTAGTAAC CAAAAGCAAG 
GCGCCTACAA AGATTAAAAA AGAGAAAACG CCTTCTGTTT CAGAAACAAA AACAGAAGAA 
GAAGAGAATG CTCCTTCTTC CACGAGTTCT TCTTCTATTT TCGACATAAA GAAAGAAGAT 
AAAGATGAGG GCGAACTGAG TAAGATTTCG AACAAGTTTA AAAAAATTAG CACGATTTTT 
GACAAAATGG GTTCTACTTC CGCTACATCG AAGGAAAATA CACCAGAACA GGACGATGTA 
GCCACTAAAA AAAATCAAAC AACCGCTAAA AAAACAGCTG TAAAACCTAA ATTGGCCAAG 
AAGCCAGTCA GGAAACAACA AAAAGTTGTG GAACTATCTG GTGAAAGCGA CCTAGAAATT 
TTAGATTCAT ACACTGATCG GGAAGATAGC AATAAAGATG AAGATGATGC TATACCACAA 
CGATCAAGGA GACAAAGATC GTCGAGAGCT GCGTCGGTTC CTAAGAAATC TTACGTTGAA 
ACTTTAGAAT TATCTGACGA CAGTTTCATC GAAGATGATG AAGAGGAAAA CCAAGGATCA 
GATGTTTCGT TCAATGAAGA GGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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TOP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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