ORF » Species Summary » Bos taurus » TOP2B cDNA ORF clone

TOP2B cDNA ORF clone, Bos taurus(cattle)

  • Gene

  • Clones

  • gRNAs

The following TOP2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231435 XM_024986380.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231436 XM_024986381.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231437 XM_024986382.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231438 XM_024986385.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231439 XM_024986386.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa45374 XM_024986383.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45374 XM_010820384.2
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45374 XM_015469767.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45374 XM_010820381.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45375 XM_024986384.1
Latest version!		 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45375 XM_010820382.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45375 XM_015469765.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45376 XM_010820383.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45376 XM_015469766.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45377 XM_015469768.1
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45377 XM_010820385.2
Latest version!		 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    TOP2B ( XM_024986380.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842148.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa231435
    Clone ID Related Accession (Same CDS sequence) XM_024986380.1
    Accession Version XM_024986380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA 
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT 
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT 
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT 
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT 
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT 
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT 
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT 
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA 
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA 
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA 
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT 
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT 
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT 
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA 
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT 
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC 
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC 
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC 
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT 
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG 
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT 
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC 
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC 
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA 
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA 
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT 
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG 
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG 
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG 
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG 
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT 
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA 
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA 
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC 
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT 
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA 
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG 
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG 
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT 
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG 
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT 
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC 
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT 
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC 
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG 
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT 
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT 
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG 
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT 
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA 
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT 
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA 
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC 
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC 
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT 
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA 
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA 
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC 
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG 
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC 
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT 
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG 
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA 
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG 
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA 
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG 
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC 
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG 
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG 
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC 
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA 
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA 
    AGTAAAACAG TAGCTGCTAA AAAGGGAAAA CCGTCTTTAG ATACAGCCTC TAAGCCCAAG 
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC 
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT 
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG 
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC 
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT 
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842148.1
    CDS37..4881
    Translation

    Target ORF information:

    RefSeq Version XM_024986380.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA 
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT 
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT 
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT 
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT 
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT 
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT 
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT 
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA 
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA 
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA 
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT 
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT 
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT 
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA 
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT 
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC 
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC 
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC 
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT 
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG 
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT 
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC 
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC 
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA 
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA 
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT 
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG 
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG 
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG 
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG 
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT 
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA 
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA 
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC 
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT 
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA 
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG 
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG 
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT 
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG 
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT 
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC 
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT 
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC 
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG 
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT 
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT 
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG 
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT 
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA 
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT 
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA 
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC 
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC 
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT 
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA 
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA 
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC 
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG 
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC 
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT 
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG 
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA 
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG 
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA 
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG 
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC 
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG 
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG 
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC 
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA 
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA 
    AGTAAAACAG TAGCTGCTAA AAAGGGAAAA CCGTCTTTAG ATACAGCCTC TAAGCCCAAG 
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC 
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT 
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG 
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC 
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT 
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_024986381.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842149.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa231436
    Clone ID Related Accession (Same CDS sequence) XM_024986381.1
    Accession Version XM_024986381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC 
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC 
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC 
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG 
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT 
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA 
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT 
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA 
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG 
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA 
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC 
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG 
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT 
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT 
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA 
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA 
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA 
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC 
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA 
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG 
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA 
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA 
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA 
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG 
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA 
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT 
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT 
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT 
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA 
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA 
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT 
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC 
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA 
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC 
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT 
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT 
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA 
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC 
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT 
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT 
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG 
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG 
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC 
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA 
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG 
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA 
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT 
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA 
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG 
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA 
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG 
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA 
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA 
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG 
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT 
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA 
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT 
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG 
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC 
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA 
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA 
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA 
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA 
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT 
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA 
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC 
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA 
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC 
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT 
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA 
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA 
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG 
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC 
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT 
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG 
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC 
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT 
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842149.1
    CDS26..4870
    Translation

    Target ORF information:

    RefSeq Version XM_024986381.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC 
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC 
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC 
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG 
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT 
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA 
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT 
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA 
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG 
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA 
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC 
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG 
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT 
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT 
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA 
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA 
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA 
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC 
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA 
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG 
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA 
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA 
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA 
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG 
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA 
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT 
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT 
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT 
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA 
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA 
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT 
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC 
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA 
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC 
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT 
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT 
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA 
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC 
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT 
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT 
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG 
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG 
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC 
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA 
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG 
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA 
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT 
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA 
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG 
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA 
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG 
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA 
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA 
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG 
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT 
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA 
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT 
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG 
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC 
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA 
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA 
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA 
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA 
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT 
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA 
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC 
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA 
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC 
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT 
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA 
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA 
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG 
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC 
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT 
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG 
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC 
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT 
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_024986382.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842150.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa231437
    Clone ID Related Accession (Same CDS sequence) XM_024986382.1
    Accession Version XM_024986382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA 
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT 
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT 
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT 
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT 
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT 
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT 
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT 
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA 
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA 
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA 
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT 
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT 
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT 
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA 
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT 
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC 
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC 
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC 
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT 
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG 
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT 
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC 
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC 
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA 
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA 
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT 
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG 
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG 
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG 
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG 
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT 
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA 
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA 
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC 
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT 
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA 
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG 
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG 
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT 
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG 
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT 
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC 
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT 
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC 
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG 
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT 
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT 
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG 
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT 
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA 
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT 
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA 
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC 
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC 
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT 
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA 
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA 
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC 
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG 
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC 
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT 
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG 
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA 
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG 
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA 
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG 
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC 
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG 
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG 
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC 
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA 
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA 
    AGTAAAACAG TAGCTGCTAA AAAGGATACA GCCTCTAAGC CCAAGAGGGC CCCCAAACAG 
    AAGAAAGTAG AGACTGTGAA CTCTGACTCG GATTCTGAAT TCGGCGTTCC AAAGAAAACA 
    GCACCGAAAG GTAAAGGCCG AGGGGCAAAG AAAAGGAAAG CATCTGGCTC TGAAAACGAA 
    GGTGATTACA ATCCTGGCAG GAAAACCTCC AAACCACCAA GCAAGAAGCC GAAGAAAACC 
    TCTTTTGATG AGGACTCAGA TGTGGACCTC TTCCCCTCAG ACCTCACCAC CGAGCCACCG 
    TCACTGCCGC GGACTGGCCG GGCAAGGAAA GAAGTAAAAT ATTTTGCAGA CTCTGACGAG 
    GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842150.1
    CDS37..4866
    Translation

    Target ORF information:

    RefSeq Version XM_024986382.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA 
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT 
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT 
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT 
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT 
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT 
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT 
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT 
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA 
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA 
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA 
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT 
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT 
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT 
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA 
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT 
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC 
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC 
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC 
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT 
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG 
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT 
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC 
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC 
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA 
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA 
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT 
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG 
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG 
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG 
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG 
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT 
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA 
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA 
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC 
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT 
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA 
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG 
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG 
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT 
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG 
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT 
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC 
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT 
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC 
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG 
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT 
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT 
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG 
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT 
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA 
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT 
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA 
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC 
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC 
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT 
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA 
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA 
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC 
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG 
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC 
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT 
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG 
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA 
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG 
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA 
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG 
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC 
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG 
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG 
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC 
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA 
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA 
    AGTAAAACAG TAGCTGCTAA AAAGGATACA GCCTCTAAGC CCAAGAGGGC CCCCAAACAG 
    AAGAAAGTAG AGACTGTGAA CTCTGACTCG GATTCTGAAT TCGGCGTTCC AAAGAAAACA 
    GCACCGAAAG GTAAAGGCCG AGGGGCAAAG AAAAGGAAAG CATCTGGCTC TGAAAACGAA 
    GGTGATTACA ATCCTGGCAG GAAAACCTCC AAACCACCAA GCAAGAAGCC GAAGAAAACC 
    TCTTTTGATG AGGACTCAGA TGTGGACCTC TTCCCCTCAG ACCTCACCAC CGAGCCACCG 
    TCACTGCCGC GGACTGGCCG GGCAAGGAAA GAAGTAAAAT ATTTTGCAGA CTCTGACGAG 
    GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_024986385.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842153.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OBa231438
    Clone ID Related Accession (Same CDS sequence) XM_024986385.1
    Accession Version XM_024986385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG 
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT 
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA 
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT 
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA 
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG 
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA 
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC 
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG 
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT 
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT 
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA 
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA 
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA 
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC 
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA 
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG 
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA 
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA 
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA 
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG 
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA 
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT 
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT 
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT 
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA 
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA 
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT 
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC 
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA 
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC 
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT 
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT 
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA 
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC 
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT 
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT 
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG 
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG 
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC 
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA 
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG 
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA 
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT 
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA 
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG 
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA 
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG 
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA 
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA 
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG 
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT 
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA 
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT 
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG 
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC 
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA 
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA 
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA 
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA 
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT 
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA 
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC 
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA 
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC 
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT 
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA 
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA 
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG 
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC 
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT 
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG 
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC 
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT 
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842153.1
    CDS265..4869
    Translation

    Target ORF information:

    RefSeq Version XM_024986385.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG 
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT 
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA 
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT 
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA 
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG 
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA 
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC 
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG 
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT 
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT 
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA 
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA 
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA 
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC 
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA 
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG 
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA 
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA 
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA 
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG 
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA 
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT 
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT 
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT 
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA 
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA 
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT 
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC 
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA 
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC 
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT 
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT 
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA 
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC 
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT 
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT 
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG 
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG 
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC 
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA 
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG 
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA 
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT 
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA 
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG 
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA 
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG 
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA 
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA 
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG 
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT 
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA 
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT 
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG 
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC 
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA 
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA 
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA 
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA 
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT 
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA 
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC 
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA 
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC 
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT 
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA 
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA 
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG 
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC 
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT 
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG 
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC 
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT 
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_024986386.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842154.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OBa231439
    Clone ID Related Accession (Same CDS sequence) XM_024986386.1
    Accession Version XM_024986386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC 
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC 
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC 
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG 
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT 
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA 
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT 
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA 
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG 
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA 
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC 
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG 
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT 
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT 
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA 
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA 
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA 
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC 
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA 
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG 
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA 
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA 
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA 
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG 
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA 
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT 
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT 
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT 
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA 
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA 
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT 
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC 
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA 
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC 
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT 
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT 
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA 
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC 
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT 
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT 
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG 
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG 
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC 
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA 
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG 
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA 
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT 
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA 
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG 
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA 
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG 
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA 
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA 
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG 
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT 
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA 
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT 
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG 
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC 
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA 
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA 
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA 
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA 
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT 
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA 
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC 
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA 
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC 
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT 
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA 
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA 
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG GAAAACCGTC TTTAGATACA 
    GCCTCTAAGC CCAAGAGGGC CCCCAAACAG AAGAAAGTAG AGACTGTGAA CTCTGACTCG 
    GATTCTGAAT TCGGCGTTCC AAAGAAAACA GCACCGAAAG GTAAAGGCCG AGGGGCAAAG 
    AAAAGGAAAG CATCTGGCTC TGAAAACGAA GGTGATTACA ATCCTGGCAG GAAAACCTCC 
    AAACCACCAA GCAAGAAGCC GAAGAAAACC TCTTTTGATG AGGACTCAGA TGTGGACCTC 
    TTCCCCTCAG ACCTCACCAC CGAGCCACCG TCACTGCCGC GGACTGGCCG GGCAAGGAAA 
    GAAGTAAAAT ATTTTGCAGA CTCTGACGAG GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842154.1
    CDS178..5037
    Translation

    Target ORF information:

    RefSeq Version XM_024986386.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC 
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC 
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC 
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG 
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT 
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA 
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT 
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA 
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG 
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA 
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC 
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG 
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT 
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT 
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA 
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA 
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA 
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC 
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA 
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG 
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA 
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA 
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA 
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG 
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA 
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT 
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT 
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT 
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA 
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA 
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT 
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC 
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA 
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC 
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT 
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT 
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA 
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC 
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT 
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT 
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG 
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG 
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC 
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA 
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG 
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA 
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT 
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA 
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG 
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA 
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG 
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA 
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA 
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG 
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT 
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA 
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT 
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG 
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC 
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA 
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA 
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA 
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA 
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT 
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA 
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA 
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC 
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA 
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC 
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT 
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA 
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA 
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG GAAAACCGTC TTTAGATACA 
    GCCTCTAAGC CCAAGAGGGC CCCCAAACAG AAGAAAGTAG AGACTGTGAA CTCTGACTCG 
    GATTCTGAAT TCGGCGTTCC AAAGAAAACA GCACCGAAAG GTAAAGGCCG AGGGGCAAAG 
    AAAAGGAAAG CATCTGGCTC TGAAAACGAA GGTGATTACA ATCCTGGCAG GAAAACCTCC 
    AAACCACCAA GCAAGAAGCC GAAGAAAACC TCTTTTGATG AGGACTCAGA TGTGGACCTC 
    TTCCCCTCAG ACCTCACCAC CGAGCCACCG TCACTGCCGC GGACTGGCCG GGCAAGGAAA 
    GAAGTAAAAT ATTTTGCAGA CTCTGACGAG GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_015469767.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325253.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X8, mRNA.
    Price & Availability↑
    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_015469767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325253.1
    CDS176..4972
    Translation

    Target ORF information:

    RefSeq Version XM_015469767.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015469767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_010820381.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818683.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_010820381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818683.1
    CDS16..4812
    Translation

    Target ORF information:

    RefSeq Version XM_010820381.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010820381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_010820384.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818686.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_010820384.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818686.1
    CDS176..4972
    Translation

    Target ORF information:

    RefSeq Version XM_010820384.2
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010820384.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_024986383.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842151.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X4, mRNA.
    Price & Availability↑
    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_024986383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842151.1
    CDS257..5053
    Translation

    Target ORF information:

    RefSeq Version XM_024986383.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC 
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT 
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA 
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA 
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC 
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA 
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_015469765.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325251.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X6, mRNA.
    Price & Availability↑
    CloneID OBa45375
    Clone ID Related Accession (Same CDS sequence) XM_015469765.1 , XM_010820382.1 , XM_024986384.1
    Accession Version XM_015469765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT 
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA 
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT 
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA 
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA 
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA 
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT 
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC 
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT 
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT 
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG 
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA 
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA 
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA 
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG 
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA 
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC 
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA 
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC 
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG 
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT 
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG 
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC 
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA 
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA 
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT 
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG 
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA 
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA 
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA 
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT 
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG 
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT 
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC 
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA 
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG 
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG 
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT 
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT 
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT 
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA 
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC 
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT 
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG 
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC 
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA 
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG 
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC 
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG 
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA 
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT 
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG 
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA 
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG 
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA 
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT 
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA 
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT 
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG 
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT 
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC 
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA 
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA 
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA 
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA 
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA 
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT 
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG 
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA 
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT 
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA 
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT 
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325251.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_015469765.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015469765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT 
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA 
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT 
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA 
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA 
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA 
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT 
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC 
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT 
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT 
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG 
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA 
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA 
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA 
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG 
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA 
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC 
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA 
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC 
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG 
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT 
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG 
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC 
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA 
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA 
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT 
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG 
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA 
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA 
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA 
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT 
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG 
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT 
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC 
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA 
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG 
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG 
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT 
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT 
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT 
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA 
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC 
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT 
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG 
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC 
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA 
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG 
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC 
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG 
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA 
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT 
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG 
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA 
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG 
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA 
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT 
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA 
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT 
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG 
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT 
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC 
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA 
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA 
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA 
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA 
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA 
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT 
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG 
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA 
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT 
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA 
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT 
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_010820382.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818684.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OBa45375
    Clone ID Related Accession (Same CDS sequence) XM_015469765.1 , XM_010820382.1 , XM_024986384.1
    Accession Version XM_010820382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT 
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA 
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT 
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA 
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA 
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA 
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT 
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC 
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT 
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT 
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG 
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA 
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA 
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA 
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG 
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA 
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC 
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA 
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC 
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG 
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT 
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG 
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC 
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA 
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA 
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT 
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG 
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA 
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA 
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA 
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT 
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG 
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT 
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC 
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA 
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG 
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG 
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT 
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT 
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT 
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA 
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC 
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT 
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG 
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC 
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA 
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG 
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC 
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG 
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA 
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT 
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG 
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA 
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG 
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA 
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT 
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA 
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT 
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG 
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT 
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC 
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA 
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA 
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA 
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA 
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA 
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT 
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG 
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA 
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT 
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA 
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT 
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818684.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_010820382.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010820382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT 
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA 
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT 
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA 
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA 
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA 
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT 
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC 
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT 
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT 
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG 
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA 
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA 
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA 
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG 
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA 
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC 
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA 
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC 
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG 
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT 
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG 
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC 
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA 
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA 
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT 
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG 
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA 
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA 
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA 
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT 
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG 
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT 
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC 
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA 
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG 
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG 
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT 
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT 
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT 
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA 
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC 
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT 
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG 
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC 
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA 
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG 
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC 
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG 
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA 
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT 
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG 
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA 
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG 
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA 
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT 
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA 
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT 
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG 
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT 
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC 
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA 
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA 
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA 
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA 
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA 
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT 
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG 
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA 
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT 
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA 
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT 
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_024986384.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842152.1 Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OBa45375
    Clone ID Related Accession (Same CDS sequence) XM_015469765.1 , XM_010820382.1 , XM_024986384.1
    Accession Version XM_024986384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT 
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA 
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT 
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA 
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA 
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA 
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT 
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC 
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT 
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT 
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG 
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA 
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA 
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA 
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG 
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA 
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC 
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA 
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC 
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG 
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT 
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG 
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC 
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA 
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA 
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT 
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG 
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA 
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA 
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA 
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT 
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG 
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT 
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC 
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA 
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG 
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG 
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT 
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT 
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT 
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA 
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC 
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT 
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG 
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC 
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA 
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG 
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC 
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG 
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA 
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT 
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG 
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA 
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG 
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA 
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT 
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA 
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT 
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG 
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT 
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC 
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA 
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA 
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA 
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA 
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA 
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT 
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG 
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA 
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT 
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA 
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT 
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842152.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_024986384.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_024986384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT 
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA 
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT 
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA 
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA 
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA 
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT 
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC 
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT 
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT 
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG 
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA 
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA 
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA 
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG 
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA 
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC 
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA 
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC 
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG 
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT 
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG 
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC 
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA 
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA 
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT 
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG 
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA 
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA 
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA 
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT 
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG 
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT 
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC 
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA 
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG 
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG 
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT 
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT 
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT 
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA 
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC 
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT 
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG 
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC 
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA 
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG 
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC 
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG 
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA 
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT 
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG 
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA 
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG 
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA 
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT 
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA 
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT 
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG 
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT 
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC 
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA 
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA 
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA 
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA 
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA 
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT 
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG 
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA 
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT 
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA 
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT 
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_010820383.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818685.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OBa45376
    Clone ID Related Accession (Same CDS sequence) XM_010820383.1 , XM_015469766.1
    Accession Version XM_010820383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818685.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_010820383.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010820383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_015469766.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325252.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X7, mRNA.
    Price & Availability↑
    CloneID OBa45376
    Clone ID Related Accession (Same CDS sequence) XM_010820383.1 , XM_015469766.1
    Accession Version XM_015469766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325252.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_015469766.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015469766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT 
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT 
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA 
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG 
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT 
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC 
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG 
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC 
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA 
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_015469768.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015325254.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X9, mRNA.
    Price & Availability↑
    CloneID OBa45377
    Clone ID Related Accession (Same CDS sequence) XM_015469768.1 , XM_010820385.2
    Accession Version XM_015469768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325254.1
    CDS16..4251
    Translation

    Target ORF information:

    RefSeq Version XM_015469768.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_015469768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    TOP2B ( XM_010820385.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010818687.1 Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X5, mRNA.
    Price & Availability↑
    CloneID OBa45377
    Clone ID Related Accession (Same CDS sequence) XM_015469768.1 , XM_010820385.2
    Accession Version XM_010820385.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818687.1
    CDS16..4251
    Translation

    Target ORF information:

    RefSeq Version XM_010820385.2
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_010820385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA 
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG 
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA 
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC 
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT 
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT 
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT 
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT 
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT 
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA 
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA 
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA 
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA 
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC 
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA 
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT 
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG 
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG 
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA 
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA 
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG 
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA 
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT 
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC 
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT 
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT 
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG 
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC 
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT 
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT 
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT 
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA 
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA 
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG 
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT 
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA 
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA 
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC 
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG 
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG 
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT 
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT 
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT 
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA 
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG 
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA 
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT 
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA 
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA 
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA 
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG 
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG 
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT 
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA 
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT 
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG 
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA 
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG 
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA 
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG 
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG 
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA 
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC 
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA 
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA 
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG 
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT 
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT 
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA 
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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