TOP2B cDNA ORF clone, Bos taurus(cattle)

The following TOP2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231435 XM_024986380.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231436 XM_024986381.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231437 XM_024986382.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231438 XM_024986385.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa231439 XM_024986386.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa45374 XM_024986383.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45374 XM_010820384.2
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45374 XM_015469767.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45374 XM_010820381.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45375 XM_024986384.1
Latest version!
Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45375 XM_010820382.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45375 XM_015469765.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45376 XM_010820383.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45376 XM_015469766.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa45377 XM_015469768.1
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa45377 XM_010820385.2
Latest version!
Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231435
    Clone ID Related Accession (Same CDS sequence) XM_024986380.1
    Accession Version XM_024986380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA
    AGTAAAACAG TAGCTGCTAA AAAGGGAAAA CCGTCTTTAG ATACAGCCTC TAAGCCCAAG
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842148.1
    CDS37..4881
    Translation

    Target ORF information:

    RefSeq Version XM_024986380.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA
    AGTAAAACAG TAGCTGCTAA AAAGGGAAAA CCGTCTTTAG ATACAGCCTC TAAGCCCAAG
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231436
    Clone ID Related Accession (Same CDS sequence) XM_024986381.1
    Accession Version XM_024986381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4845bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842149.1
    CDS26..4870
    Translation

    Target ORF information:

    RefSeq Version XM_024986381.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231437
    Clone ID Related Accession (Same CDS sequence) XM_024986382.1
    Accession Version XM_024986382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4830bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA
    AGTAAAACAG TAGCTGCTAA AAAGGATACA GCCTCTAAGC CCAAGAGGGC CCCCAAACAG
    AAGAAAGTAG AGACTGTGAA CTCTGACTCG GATTCTGAAT TCGGCGTTCC AAAGAAAACA
    GCACCGAAAG GTAAAGGCCG AGGGGCAAAG AAAAGGAAAG CATCTGGCTC TGAAAACGAA
    GGTGATTACA ATCCTGGCAG GAAAACCTCC AAACCACCAA GCAAGAAGCC GAAGAAAACC
    TCTTTTGATG AGGACTCAGA TGTGGACCTC TTCCCCTCAG ACCTCACCAC CGAGCCACCG
    TCACTGCCGC GGACTGGCCG GGCAAGGAAA GAAGTAAAAT ATTTTGCAGA CTCTGACGAG
    GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842150.1
    CDS37..4866
    Translation

    Target ORF information:

    RefSeq Version XM_024986382.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGAACA ATGCTGCAAA AAAGGAAGAG TCAGAAATTG CAAACAAAAA TGATTCTTCA
    AAGAAGTTGT CCGTCGAGAG AGTGTATCAG AAAAAGACAC AACTCGAACA TATTCTGCTT
    CGTCCTGATA CGTACATTGG GTCAGTGGAG CCATTGACAC AGCTCATGTG GGTATATGAT
    GAAGATGTAG GAATGAATTG CAGAGAGGTT ACCTTTGTGC CGGGTTTATA TAAGATCTTT
    GATGAAATTT TGGTTAATGC AGCTGATAAT AAACAGAGGG ATAAGAACAT GACCTGTATT
    AAAGTTTCTA TTGATCCTGA ATCTAACATA ATAAGCATTT GGAATAATGG GAAAGGCATT
    CCAGTAGTAG AACACAAAGT AGAGAAAGTT TATGTTCCTG CATTAATTTT TGGACAGCTT
    TTAACGTCCA GTAACTATGA CGATGATGAG AAAAAAGTAA CAGGTGGTCG CAATGGTTAT
    GGTGCAAAAC TTTGTAATAT TTTCAGTACA AAGTTTACAG TAGAAACTGC TTGCAAAGAA
    TACAAACATA GTTTTAAGCA GACATGGATG AATAATATGA TGAAGACTTC TGAAGCCAAA
    ATTAAACATT TTGATGGTGA AGATTACACA TGTATAACAT TCCAACCAGA TCTGTCCAAA
    TTTAAGATGG AGAAGCTTGA CAGGGATATT GTTGCTCTCA TGACCAGAAG AGCTTATGAT
    TTGGCTGGTT CCTGCAAGGG AGTCAAGGTC ATGTTTAATG GAAAGAAATT GCCGGTAAAT
    GGATTTCGCA GTTATGTAGA TCTTTATGTG AAAGACAAAT TGGATGAAAC GGGAGTGGCT
    CTAAAAGTTA TTCATGAACT TGCAAATGAA AGATGGGACG TTTGTCTCAC TTTGAGTGAA
    AAAGGATTCC AGCAAATCAG CTTTGTAAAT AGCATTGCAA CAACAAAAGG TGGACGGCAT
    GTGGATTACG TAGTAGACCA AGTTGTTGGT AAACTGATTG AAGTAGTTAA GAAAAAGAAC
    AAAGCTGGTG TGTCAGTGAA ACCATTTCAA GTAAAAAATC ATATATGGGT TTTTATTAAC
    TGCCTTATTG AAAATCCAAC TTTTGATTCT CAAACTAAGG AAAACATGAC TTTGCAGCCC
    AAAAGTTTTG GATCCAAATG CCAACTATCA GAAAAATTTT TTAAAGCAGC CTCTAACTGT
    GGCATTGTAG AAAGTATCCT GAACTGGGTG AAATTTAAGG CTCAGACACA GCTGAACAAG
    AAGTGTTCAT CAGTAAAGTA CAGTAAAATC AAAGGTATTC CCAAATTGGA TGATGCTAAT
    GATGCTGGTG GCAAACATTC CCTGGAGTGC ACACTGATCC TGACAGAAGG AGACTCTGCC
    AAGTCCCTGG CCGTGTCTGG ATTAGGCGTC ATTGGGAGGG ACAGATATGG GGTCTTTCCC
    CTCAGGGGAA AGATTCTTAA CGTGCGGGAA GCTTCTCACA AACAGATCAT GGAAAATGCA
    GAAATAAACA ATATTATTAA AATAGTTGGT CTACAATATA AGAAAAGTTA TGATGATGCA
    GAATCTCTGA AAACTTTACG CTATGGAAAG ATTATGATTA TGACTGATCA GGATCAAGAT
    GGTTCTCACA TAAAAGGCCT GCTTATTAAT TTCATCCATC ACAATTGGCC ATCACTTTTG
    AAGCATGGTT TTCTTGAAGA GTTCATTACC CCTATTGTAA AGGCTAGCAA AAATAAGCAG
    GAACTTTCCT TCTACAGTAT TCCTGAATTT GATGAGTGGA AAAAACATAT AGAAAACCAG
    AAAGCCTGGA AAATAAAATA CTACAAAGGG TTGGGTACCA GTACAGCTAA GGAAGCCAAG
    GAATATTTTG CTGATATGGA AAGGCACCGT ATCTTATTTA GATATGCTGG TCCTGAAGAT
    GATGCTGCCA TTACCTTGGC ATTCAGTAAG AAGAAGATCG ATGACAGAAA AGAATGGTTA
    ACGAACTTTA TGGAAGATCG GAGACAGCGT AGGTTACATG GCTTACCAGA GCAATTCTTA
    TATGGTACAG CAACCAAGCA TTTGACTTAT AATGATTTTA TCAACAAGGA ATTGATTCTC
    TTCTCAAACT CAGACAATGA AAGATCTATA CCATCTCTTG TTGATGGTTT TAAACCAGGT
    CAACGAAAAG TTTTATTTAC CTGTTTCAAG AGGAATGATA AACGTGAAGT GAAAGTTGCA
    CAGTTGGCTG GATCTGTTGC CGAGATGTCT GCTTATCATC ATGGAGAACA AGCATTGATG
    ATGACTATTG TGAACTTGGC TCAGAACTTT GTGGGGAGCA ACAACATCAA CTTGCTTCAG
    CCTATTGGTC AGTTTGGAAC TCGACTTCAC GGGGGCAAAG ATGCTGCGAG CCCTCGTTAT
    ATTTTCACAA TGTTGAGCAC TTTAGCAAGA CTGCTTTTTC CTGCTGTGGA TGACAACCTG
    CTTAAATTCC TTTATGATGA TAATCAGCGT GTGGAGCCTG AGTGGTATAT TCCCATAATT
    CCAATGGTTT TAATAAATGG TGCTGAGGGC ATTGGCACAG GGTGGGCTTG TAAGCTGCCC
    AACTACGATG CCAGAGAGAT TGTGAACAAT GTCAGGCGAA TGCTCGATGG CCTGGATCCT
    CATCCCATGC TTCCAAACTA CAAAAACTTT AAAGGAACGA TCCAAGAACT TGGTCAAAAC
    CAGTATGCAG TCAGTGGTGA GATATTTGTG GTGGATAGAA ACACGGTGGA GATTACCGAG
    CTCCCCGTCA GAACTTGGAC ACAGGTATAC AAAGAACAGG TTTTAGAACC TATGCTAAAT
    GGAACAGATA AAACACCAGC ATTAATTTCT GATTATAAAG AATATCATAC TGATACAACT
    GTGAAATTTG TAGTGAAAAT GACTGAAGAG AAGCTAGCAC AAGCAGAAGC TGCTGGATTG
    CACAAAGTTT TTAAACTTCA AACTACTCTT ACTTGTAATT CCATGGTACT CTTTGATCAT
    ATGGGATGTC TGAAGAAGTA TGAAACTGTG CAAGATATTT TGAAAGAATT CTTTGATTTA
    CGATTAAGCT ATTATGGTTT ACGAAAGGAG TGGCTTGTAG GAATGTTGGG AGCAGAATCT
    ACTAAGCTTA ATAATCAAGC CCGTTTCATT TTAGAGAAGA TACAAGGGAA AATCACTATA
    GAGAATAGGT CAAAGAAAGA TTTGATTCAA ATGTTAGTCC AGAGAGGTTA TGAATCTGAC
    CCAGTGAAAG CCTGGAAAGA AGCACAAGAA AAGGCAGCAG AAGAGGAAGA ATCACAAAAC
    CAGCATGATG ATAGTTCCTC TGATTCAGGA ACTCCTTCAG GCCCTGATTT TAATTATATT
    TTAAATATGT CTCTATGGTC TCTTACTAAA GAAAAAGTTG AAGAACTGAT TAAACAGAGA
    GATGCAAAAG GGCGAGAGGT CAGTGATCTT AAAAGGAAAT CTCCTTCAGA TCTTTGGAAA
    GAAGATTTAG CAGCTTTTGT TGAAGAACTG GATAAAGTGG AAGCGCAGGA ACGAGAAGAC
    GTTCTGGCCG GGATGGCCGG AAAAGCAATC AAGGGGAAGG TTGGCAAACC TAAGGTGAAG
    AAGCTTCAGC TAGAAGAGAC GATGCCCTCC CCTTACGGCA GGAGAGTGGT TCCTGAGATC
    ACTGCCATGA AGGCAGATGC CAGCAAGAAG CTGCTGAAGA AGAGAAAGGG CGATCTGGAT
    GCCACAGCAG TCCGAGTGGA CTTTGATGAA GAGCTCAGCG GAGCAGCAGT GGAAGGTGGG
    GAAGAGGCGC CGATCCCGTC AGCTCCTGTA CACAGAGGCC CCAAACCCAA AAGGGAGAAA
    AAAGAGCCTG GTACCAGGGT GAGAAAAACA CCTACATCAT CTGGTAAACC GAGTGCAAAG
    AAAGTGAAGA AACGGAATCC TTGGTCAGAT GATGAATCCA AGTCAGAAAG TGACTTGGAA
    GAAACAGAAC CTGTGGTTAT TCCAAGAGAC TCTTTACTTA GGAGAGCGGC TGCTGAACGG
    CCAAAGTATA CATTCGATTT CTCGGAAGAG GAGGAAGATG ACGCCGATGA TGATGACGAC
    AATAATGACT TAGAGGAGCT GAAGGTCAAA GCATCTCCCA TAGCAAATGA TGGAGAAGAG
    GAGTTTGTCC CCTCAGATGG CCTAGATAAA GATGAATACA CATTCTCACC AGGCAAATCG
    AAAGCTGCTC CAGAAAAGTC TTCCCATGAC AAGAAGAGTC AGGATTTTGG GACTCTCTTC
    TCTTTTCCTT CATACTCTCA GAAGTCAGAA GATGATTCAG CTAAATTTGA CAGTAATGAA
    GAAGATTCTG CTTCTGTATT TTCACCATCA TTTGGTCTGA AGCAAACAGA AAAAGTTCCA
    AGTAAAACAG TAGCTGCTAA AAAGGATACA GCCTCTAAGC CCAAGAGGGC CCCCAAACAG
    AAGAAAGTAG AGACTGTGAA CTCTGACTCG GATTCTGAAT TCGGCGTTCC AAAGAAAACA
    GCACCGAAAG GTAAAGGCCG AGGGGCAAAG AAAAGGAAAG CATCTGGCTC TGAAAACGAA
    GGTGATTACA ATCCTGGCAG GAAAACCTCC AAACCACCAA GCAAGAAGCC GAAGAAAACC
    TCTTTTGATG AGGACTCAGA TGTGGACCTC TTCCCCTCAG ACCTCACCAC CGAGCCACCG
    TCACTGCCGC GGACTGGCCG GGCAAGGAAA GAAGTAAAAT ATTTTGCAGA CTCTGACGAG
    GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231438
    Clone ID Related Accession (Same CDS sequence) XM_024986385.1
    Accession Version XM_024986385.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842153.1
    CDS265..4869
    Translation

    Target ORF information:

    RefSeq Version XM_024986385.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986385.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT 
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG ATACAGCCTC TAAGCCCAAG
    AGGGCCCCCA AACAGAAGAA AGTAGAGACT GTGAACTCTG ACTCGGATTC TGAATTCGGC
    GTTCCAAAGA AAACAGCACC GAAAGGTAAA GGCCGAGGGG CAAAGAAAAG GAAAGCATCT
    GGCTCTGAAA ACGAAGGTGA TTACAATCCT GGCAGGAAAA CCTCCAAACC ACCAAGCAAG
    AAGCCGAAGA AAACCTCTTT TGATGAGGAC TCAGATGTGG ACCTCTTCCC CTCAGACCTC
    ACCACCGAGC CACCGTCACT GCCGCGGACT GGCCGGGCAA GGAAAGAAGT AAAATATTTT
    GCAGACTCTG ACGAGGAAGA TGTCGATTTT GCGATGTTTA ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231439
    Clone ID Related Accession (Same CDS sequence) XM_024986386.1
    Accession Version XM_024986386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4860bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG GAAAACCGTC TTTAGATACA
    GCCTCTAAGC CCAAGAGGGC CCCCAAACAG AAGAAAGTAG AGACTGTGAA CTCTGACTCG
    GATTCTGAAT TCGGCGTTCC AAAGAAAACA GCACCGAAAG GTAAAGGCCG AGGGGCAAAG
    AAAAGGAAAG CATCTGGCTC TGAAAACGAA GGTGATTACA ATCCTGGCAG GAAAACCTCC
    AAACCACCAA GCAAGAAGCC GAAGAAAACC TCTTTTGATG AGGACTCAGA TGTGGACCTC
    TTCCCCTCAG ACCTCACCAC CGAGCCACCG TCACTGCCGC GGACTGGCCG GGCAAGGAAA
    GAAGTAAAAT ATTTTGCAGA CTCTGACGAG GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842154.1
    CDS178..5037
    Translation

    Target ORF information:

    RefSeq Version XM_024986386.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCCAAGG CGGGCGGCGG CGGCGGCGGC GCGGGAGCTG GCGGCCACGG GGCGCTGACC 
    TGGGTGACTC TCTTTGATCA GAACAATGCT GCAAAAAAGG AAGAGTCAGA AATTGCAAAC
    AAAAATGATT CTTCAAAGAA GTTGTCCGTC GAGAGAGTGT ATCAGAAAAA GACACAACTC
    GAACATATTC TGCTTCGTCC TGATACGTAC ATTGGGTCAG TGGAGCCATT GACACAGCTC
    ATGTGGGTAT ATGATGAAGA TGTAGGAATG AATTGCAGAG AGGTTACCTT TGTGCCGGGT
    TTATATAAGA TCTTTGATGA AATTTTGGTT AATGCAGCTG ATAATAAACA GAGGGATAAG
    AACATGACCT GTATTAAAGT TTCTATTGAT CCTGAATCTA ACATAATAAG CATTTGGAAT
    AATGGGAAAG GCATTCCAGT AGTAGAACAC AAAGTAGAGA AAGTTTATGT TCCTGCATTA
    ATTTTTGGAC AGCTTTTAAC GTCCAGTAAC TATGACGATG ATGAGAAAAA AGTAACAGGT
    GGTCGCAATG GTTATGGTGC AAAACTTTGT AATATTTTCA GTACAAAGTT TACAGTAGAA
    ACTGCTTGCA AAGAATACAA ACATAGTTTT AAGCAGACAT GGATGAATAA TATGATGAAG
    ACTTCTGAAG CCAAAATTAA ACATTTTGAT GGTGAAGATT ACACATGTAT AACATTCCAA
    CCAGATCTGT CCAAATTTAA GATGGAGAAG CTTGACAGGG ATATTGTTGC TCTCATGACC
    AGAAGAGCTT ATGATTTGGC TGGTTCCTGC AAGGGAGTCA AGGTCATGTT TAATGGAAAG
    AAATTGCCGG TAAATGGATT TCGCAGTTAT GTAGATCTTT ATGTGAAAGA CAAATTGGAT
    GAAACGGGAG TGGCTCTAAA AGTTATTCAT GAACTTGCAA ATGAAAGATG GGACGTTTGT
    CTCACTTTGA GTGAAAAAGG ATTCCAGCAA ATCAGCTTTG TAAATAGCAT TGCAACAACA
    AAAGGTGGAC GGCATGTGGA TTACGTAGTA GACCAAGTTG TTGGTAAACT GATTGAAGTA
    GTTAAGAAAA AGAACAAAGC TGGTGTGTCA GTGAAACCAT TTCAAGTAAA AAATCATATA
    TGGGTTTTTA TTAACTGCCT TATTGAAAAT CCAACTTTTG ATTCTCAAAC TAAGGAAAAC
    ATGACTTTGC AGCCCAAAAG TTTTGGATCC AAATGCCAAC TATCAGAAAA ATTTTTTAAA
    GCAGCCTCTA ACTGTGGCAT TGTAGAAAGT ATCCTGAACT GGGTGAAATT TAAGGCTCAG
    ACACAGCTGA ACAAGAAGTG TTCATCAGTA AAGTACAGTA AAATCAAAGG TATTCCCAAA
    TTGGATGATG CTAATGATGC TGGTGGCAAA CATTCCCTGG AGTGCACACT GATCCTGACA
    GAAGGAGACT CTGCCAAGTC CCTGGCCGTG TCTGGATTAG GCGTCATTGG GAGGGACAGA
    TATGGGGTCT TTCCCCTCAG GGGAAAGATT CTTAACGTGC GGGAAGCTTC TCACAAACAG
    ATCATGGAAA ATGCAGAAAT AAACAATATT ATTAAAATAG TTGGTCTACA ATATAAGAAA
    AGTTATGATG ATGCAGAATC TCTGAAAACT TTACGCTATG GAAAGATTAT GATTATGACT
    GATCAGGATC AAGATGGTTC TCACATAAAA GGCCTGCTTA TTAATTTCAT CCATCACAAT
    TGGCCATCAC TTTTGAAGCA TGGTTTTCTT GAAGAGTTCA TTACCCCTAT TGTAAAGGCT
    AGCAAAAATA AGCAGGAACT TTCCTTCTAC AGTATTCCTG AATTTGATGA GTGGAAAAAA
    CATATAGAAA ACCAGAAAGC CTGGAAAATA AAATACTACA AAGGGTTGGG TACCAGTACA
    GCTAAGGAAG CCAAGGAATA TTTTGCTGAT ATGGAAAGGC ACCGTATCTT ATTTAGATAT
    GCTGGTCCTG AAGATGATGC TGCCATTACC TTGGCATTCA GTAAGAAGAA GATCGATGAC
    AGAAAAGAAT GGTTAACGAA CTTTATGGAA GATCGGAGAC AGCGTAGGTT ACATGGCTTA
    CCAGAGCAAT TCTTATATGG TACAGCAACC AAGCATTTGA CTTATAATGA TTTTATCAAC
    AAGGAATTGA TTCTCTTCTC AAACTCAGAC AATGAAAGAT CTATACCATC TCTTGTTGAT
    GGTTTTAAAC CAGGTCAACG AAAAGTTTTA TTTACCTGTT TCAAGAGGAA TGATAAACGT
    GAAGTGAAAG TTGCACAGTT GGCTGGATCT GTTGCCGAGA TGTCTGCTTA TCATCATGGA
    GAACAAGCAT TGATGATGAC TATTGTGAAC TTGGCTCAGA ACTTTGTGGG GAGCAACAAC
    ATCAACTTGC TTCAGCCTAT TGGTCAGTTT GGAACTCGAC TTCACGGGGG CAAAGATGCT
    GCGAGCCCTC GTTATATTTT CACAATGTTG AGCACTTTAG CAAGACTGCT TTTTCCTGCT
    GTGGATGACA ACCTGCTTAA ATTCCTTTAT GATGATAATC AGCGTGTGGA GCCTGAGTGG
    TATATTCCCA TAATTCCAAT GGTTTTAATA AATGGTGCTG AGGGCATTGG CACAGGGTGG
    GCTTGTAAGC TGCCCAACTA CGATGCCAGA GAGATTGTGA ACAATGTCAG GCGAATGCTC
    GATGGCCTGG ATCCTCATCC CATGCTTCCA AACTACAAAA ACTTTAAAGG AACGATCCAA
    GAACTTGGTC AAAACCAGTA TGCAGTCAGT GGTGAGATAT TTGTGGTGGA TAGAAACACG
    GTGGAGATTA CCGAGCTCCC CGTCAGAACT TGGACACAGG TATACAAAGA ACAGGTTTTA
    GAACCTATGC TAAATGGAAC AGATAAAACA CCAGCATTAA TTTCTGATTA TAAAGAATAT
    CATACTGATA CAACTGTGAA ATTTGTAGTG AAAATGACTG AAGAGAAGCT AGCACAAGCA
    GAAGCTGCTG GATTGCACAA AGTTTTTAAA CTTCAAACTA CTCTTACTTG TAATTCCATG
    GTACTCTTTG ATCATATGGG ATGTCTGAAG AAGTATGAAA CTGTGCAAGA TATTTTGAAA
    GAATTCTTTG ATTTACGATT AAGCTATTAT GGTTTACGAA AGGAGTGGCT TGTAGGAATG
    TTGGGAGCAG AATCTACTAA GCTTAATAAT CAAGCCCGTT TCATTTTAGA GAAGATACAA
    GGGAAAATCA CTATAGAGAA TAGGTCAAAG AAAGATTTGA TTCAAATGTT AGTCCAGAGA
    GGTTATGAAT CTGACCCAGT GAAAGCCTGG AAAGAAGCAC AAGAAAAGGC AGCAGAAGAG
    GAAGAATCAC AAAACCAGCA TGATGATAGT TCCTCTGATT CAGGAACTCC TTCAGGCCCT
    GATTTTAATT ATATTTTAAA TATGTCTCTA TGGTCTCTTA CTAAAGAAAA AGTTGAAGAA
    CTGATTAAAC AGAGAGATGC AAAAGGGCGA GAGGTCAGTG ATCTTAAAAG GAAATCTCCT
    TCAGATCTTT GGAAAGAAGA TTTAGCAGCT TTTGTTGAAG AACTGGATAA AGTGGAAGCG
    CAGGAACGAG AAGACGTTCT GGCCGGGATG GCCGGAAAAG CAATCAAGGG GAAGGTTGGC
    AAACCTAAGG TGAAGAAGCT TCAGCTAGAA GAGACGATGC CCTCCCCTTA CGGCAGGAGA
    GTGGTTCCTG AGATCACTGC CATGAAGGCA GATGCCAGCA AGAAGCTGCT GAAGAAGAGA
    AAGGGCGATC TGGATGCCAC AGCAGTCCGA GTGGACTTTG ATGAAGAGCT CAGCGGAGCA
    GCAGTGGAAG GTGGGGAAGA GGCGCCGATC CCGTCAGCTC CTGTACACAG AGGCCCCAAA
    CCCAAAAGGG AGAAAAAAGA GCCTGGTACC AGGGTGAGAA AAACACCTAC ATCATCTGGT
    AAACCGAGTG CAAAGAAAGT GAAGAAACGG AATCCTTGGT CAGATGATGA ATCCAAGTCA
    GAAAGTGACT TGGAAGAAAC AGAACCTGTG GTTATTCCAA GAGACTCTTT ACTTAGGAGA
    GCGGCTGCTG AACGGCCAAA GTATACATTC GATTTCTCGG AAGAGGAGGA AGATGACGCC
    GATGATGATG ACGACAATAA TGACTTAGAG GAGCTGAAGG TCAAAGCATC TCCCATAGCA
    AATGATGGAG AAGAGGAGTT TGTCCCCTCA GATGGCCTAG ATAAAGATGA ATACACATTC
    TCACCAGGCA AATCGAAAGC TGCTCCAGAA AAGTCTTCCC ATGACAAGAA GAGTCAGGAT
    TTTGGGACTC TCTTCTCTTT TCCTTCATAC TCTCAGAAGT CAGAAGATGA TTCAGCTAAA
    TTTGACAGTA ATGAAGAAGA TTCTGCTTCT GTATTTTCAC CATCATTTGG TCTGAAGCAA
    ACAGAAAAAG TTCCAAGTAA AACAGTAGCT GCTAAAAAGG GAAAACCGTC TTTAGATACA
    GCCTCTAAGC CCAAGAGGGC CCCCAAACAG AAGAAAGTAG AGACTGTGAA CTCTGACTCG
    GATTCTGAAT TCGGCGTTCC AAAGAAAACA GCACCGAAAG GTAAAGGCCG AGGGGCAAAG
    AAAAGGAAAG CATCTGGCTC TGAAAACGAA GGTGATTACA ATCCTGGCAG GAAAACCTCC
    AAACCACCAA GCAAGAAGCC GAAGAAAACC TCTTTTGATG AGGACTCAGA TGTGGACCTC
    TTCCCCTCAG ACCTCACCAC CGAGCCACCG TCACTGCCGC GGACTGGCCG GGCAAGGAAA
    GAAGTAAAAT ATTTTGCAGA CTCTGACGAG GAAGATGTCG ATTTTGCGAT GTTTAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_015469767.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325253.1
    CDS176..4972
    Translation

    Target ORF information:

    RefSeq Version XM_015469767.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_010820381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818683.1
    CDS16..4812
    Translation

    Target ORF information:

    RefSeq Version XM_010820381.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_010820384.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818686.1
    CDS176..4972
    Translation

    Target ORF information:

    RefSeq Version XM_010820384.2
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820384.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45374
    Clone ID Related Accession (Same CDS sequence) XM_015469767.1 , XM_010820381.1 , XM_010820384.2 , XM_024986383.1
    Accession Version XM_024986383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4797bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842151.1
    CDS257..5053
    Translation

    Target ORF information:

    RefSeq Version XM_024986383.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGGAA AACCGTCTTT AGATACAGCC
    TCTAAGCCCA AGAGGGCCCC CAAACAGAAG AAAGTAGAGA CTGTGAACTC TGACTCGGAT
    TCTGAATTCG GCGTTCCAAA GAAAACAGCA CCGAAAGGTA AAGGCCGAGG GGCAAAGAAA
    AGGAAAGCAT CTGGCTCTGA AAACGAAGGT GATTACAATC CTGGCAGGAA AACCTCCAAA
    CCACCAAGCA AGAAGCCGAA GAAAACCTCT TTTGATGAGG ACTCAGATGT GGACCTCTTC
    CCCTCAGACC TCACCACCGA GCCACCGTCA CTGCCGCGGA CTGGCCGGGC AAGGAAAGAA
    GTAAAATATT TTGCAGACTC TGACGAGGAA GATGTCGATT TTGCGATGTT TAATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45375
    Clone ID Related Accession (Same CDS sequence) XM_015469765.1 , XM_010820382.1 , XM_024986384.1
    Accession Version XM_015469765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325251.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_015469765.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45375
    Clone ID Related Accession (Same CDS sequence) XM_015469765.1 , XM_010820382.1 , XM_024986384.1
    Accession Version XM_010820382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818684.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_010820382.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45375
    Clone ID Related Accession (Same CDS sequence) XM_015469765.1 , XM_010820382.1 , XM_024986384.1
    Accession Version XM_024986384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842152.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_024986384.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DNA topoisomerase II beta (TOP2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAATG CTGCAAAAAA GGAAGAGTCA GAAATTGCAA ACAAAAATGA TTCTTCAAAG 
    AAGTTGTCCG TCGAGAGAGT GTATCAGAAA AAGACACAAC TCGAACATAT TCTGCTTCGT
    CCTGATACGT ACATTGGGTC AGTGGAGCCA TTGACACAGC TCATGTGGGT ATATGATGAA
    GATGTAGGAA TGAATTGCAG AGAGGTTACC TTTGTGCCGG GTTTATATAA GATCTTTGAT
    GAAATTTTGG TTAATGCAGC TGATAATAAA CAGAGGGATA AGAACATGAC CTGTATTAAA
    GTTTCTATTG ATCCTGAATC TAACATAATA AGCATTTGGA ATAATGGGAA AGGCATTCCA
    GTAGTAGAAC ACAAAGTAGA GAAAGTTTAT GTTCCTGCAT TAATTTTTGG ACAGCTTTTA
    ACGTCCAGTA ACTATGACGA TGATGAGAAA AAAGTAACAG GTGGTCGCAA TGGTTATGGT
    GCAAAACTTT GTAATATTTT CAGTACAAAG TTTACAGTAG AAACTGCTTG CAAAGAATAC
    AAACATAGTT TTAAGCAGAC ATGGATGAAT AATATGATGA AGACTTCTGA AGCCAAAATT
    AAACATTTTG ATGGTGAAGA TTACACATGT ATAACATTCC AACCAGATCT GTCCAAATTT
    AAGATGGAGA AGCTTGACAG GGATATTGTT GCTCTCATGA CCAGAAGAGC TTATGATTTG
    GCTGGTTCCT GCAAGGGAGT CAAGGTCATG TTTAATGGAA AGAAATTGCC GGTAAATGGA
    TTTCGCAGTT ATGTAGATCT TTATGTGAAA GACAAATTGG ATGAAACGGG AGTGGCTCTA
    AAAGTTATTC ATGAACTTGC AAATGAAAGA TGGGACGTTT GTCTCACTTT GAGTGAAAAA
    GGATTCCAGC AAATCAGCTT TGTAAATAGC ATTGCAACAA CAAAAGGTGG ACGGCATGTG
    GATTACGTAG TAGACCAAGT TGTTGGTAAA CTGATTGAAG TAGTTAAGAA AAAGAACAAA
    GCTGGTGTGT CAGTGAAACC ATTTCAAGTA AAAAATCATA TATGGGTTTT TATTAACTGC
    CTTATTGAAA ATCCAACTTT TGATTCTCAA ACTAAGGAAA ACATGACTTT GCAGCCCAAA
    AGTTTTGGAT CCAAATGCCA ACTATCAGAA AAATTTTTTA AAGCAGCCTC TAACTGTGGC
    ATTGTAGAAA GTATCCTGAA CTGGGTGAAA TTTAAGGCTC AGACACAGCT GAACAAGAAG
    TGTTCATCAG TAAAGTACAG TAAAATCAAA GGTATTCCCA AATTGGATGA TGCTAATGAT
    GCTGGTGGCA AACATTCCCT GGAGTGCACA CTGATCCTGA CAGAAGGAGA CTCTGCCAAG
    TCCCTGGCCG TGTCTGGATT AGGCGTCATT GGGAGGGACA GATATGGGGT CTTTCCCCTC
    AGGGGAAAGA TTCTTAACGT GCGGGAAGCT TCTCACAAAC AGATCATGGA AAATGCAGAA
    ATAAACAATA TTATTAAAAT AGTTGGTCTA CAATATAAGA AAAGTTATGA TGATGCAGAA
    TCTCTGAAAA CTTTACGCTA TGGAAAGATT ATGATTATGA CTGATCAGGA TCAAGATGGT
    TCTCACATAA AAGGCCTGCT TATTAATTTC ATCCATCACA ATTGGCCATC ACTTTTGAAG
    CATGGTTTTC TTGAAGAGTT CATTACCCCT ATTGTAAAGG CTAGCAAAAA TAAGCAGGAA
    CTTTCCTTCT ACAGTATTCC TGAATTTGAT GAGTGGAAAA AACATATAGA AAACCAGAAA
    GCCTGGAAAA TAAAATACTA CAAAGGGTTG GGTACCAGTA CAGCTAAGGA AGCCAAGGAA
    TATTTTGCTG ATATGGAAAG GCACCGTATC TTATTTAGAT ATGCTGGTCC TGAAGATGAT
    GCTGCCATTA CCTTGGCATT CAGTAAGAAG AAGATCGATG ACAGAAAAGA ATGGTTAACG
    AACTTTATGG AAGATCGGAG ACAGCGTAGG TTACATGGCT TACCAGAGCA ATTCTTATAT
    GGTACAGCAA CCAAGCATTT GACTTATAAT GATTTTATCA ACAAGGAATT GATTCTCTTC
    TCAAACTCAG ACAATGAAAG ATCTATACCA TCTCTTGTTG ATGGTTTTAA ACCAGGTCAA
    CGAAAAGTTT TATTTACCTG TTTCAAGAGG AATGATAAAC GTGAAGTGAA AGTTGCACAG
    TTGGCTGGAT CTGTTGCCGA GATGTCTGCT TATCATCATG GAGAACAAGC ATTGATGATG
    ACTATTGTGA ACTTGGCTCA GAACTTTGTG GGGAGCAACA ACATCAACTT GCTTCAGCCT
    ATTGGTCAGT TTGGAACTCG ACTTCACGGG GGCAAAGATG CTGCGAGCCC TCGTTATATT
    TTCACAATGT TGAGCACTTT AGCAAGACTG CTTTTTCCTG CTGTGGATGA CAACCTGCTT
    AAATTCCTTT ATGATGATAA TCAGCGTGTG GAGCCTGAGT GGTATATTCC CATAATTCCA
    ATGGTTTTAA TAAATGGTGC TGAGGGCATT GGCACAGGGT GGGCTTGTAA GCTGCCCAAC
    TACGATGCCA GAGAGATTGT GAACAATGTC AGGCGAATGC TCGATGGCCT GGATCCTCAT
    CCCATGCTTC CAAACTACAA AAACTTTAAA GGAACGATCC AAGAACTTGG TCAAAACCAG
    TATGCAGTCA GTGGTGAGAT ATTTGTGGTG GATAGAAACA CGGTGGAGAT TACCGAGCTC
    CCCGTCAGAA CTTGGACACA GGTATACAAA GAACAGGTTT TAGAACCTAT GCTAAATGGA
    ACAGATAAAA CACCAGCATT AATTTCTGAT TATAAAGAAT ATCATACTGA TACAACTGTG
    AAATTTGTAG TGAAAATGAC TGAAGAGAAG CTAGCACAAG CAGAAGCTGC TGGATTGCAC
    AAAGTTTTTA AACTTCAAAC TACTCTTACT TGTAATTCCA TGGTACTCTT TGATCATATG
    GGATGTCTGA AGAAGTATGA AACTGTGCAA GATATTTTGA AAGAATTCTT TGATTTACGA
    TTAAGCTATT ATGGTTTACG AAAGGAGTGG CTTGTAGGAA TGTTGGGAGC AGAATCTACT
    AAGCTTAATA ATCAAGCCCG TTTCATTTTA GAGAAGATAC AAGGGAAAAT CACTATAGAG
    AATAGGTCAA AGAAAGATTT GATTCAAATG TTAGTCCAGA GAGGTTATGA ATCTGACCCA
    GTGAAAGCCT GGAAAGAAGC ACAAGAAAAG GCAGCAGAAG AGGAAGAATC ACAAAACCAG
    CATGATGATA GTTCCTCTGA TTCAGGAACT CCTTCAGGCC CTGATTTTAA TTATATTTTA
    AATATGTCTC TATGGTCTCT TACTAAAGAA AAAGTTGAAG AACTGATTAA ACAGAGAGAT
    GCAAAAGGGC GAGAGGTCAG TGATCTTAAA AGGAAATCTC CTTCAGATCT TTGGAAAGAA
    GATTTAGCAG CTTTTGTTGA AGAACTGGAT AAAGTGGAAG CGCAGGAACG AGAAGACGTT
    CTGGCCGGGA TGGCCGGAAA AGCAATCAAG GGGAAGGTTG GCAAACCTAA GGTGAAGAAG
    CTTCAGCTAG AAGAGACGAT GCCCTCCCCT TACGGCAGGA GAGTGGTTCC TGAGATCACT
    GCCATGAAGG CAGATGCCAG CAAGAAGCTG CTGAAGAAGA GAAAGGGCGA TCTGGATGCC
    ACAGCAGTCC GAGTGGACTT TGATGAAGAG CTCAGCGGAG CAGCAGTGGA AGGTGGGGAA
    GAGGCGCCGA TCCCGTCAGC TCCTGTACAC AGAGGCCCCA AACCCAAAAG GGAGAAAAAA
    GAGCCTGGTA CCAGGGTGAG AAAAACACCT ACATCATCTG GTAAACCGAG TGCAAAGAAA
    GTGAAGAAAC GGAATCCTTG GTCAGATGAT GAATCCAAGT CAGAAAGTGA CTTGGAAGAA
    ACAGAACCTG TGGTTATTCC AAGAGACTCT TTACTTAGGA GAGCGGCTGC TGAACGGCCA
    AAGTATACAT TCGATTTCTC GGAAGAGGAG GAAGATGACG CCGATGATGA TGACGACAAT
    AATGACTTAG AGGAGCTGAA GGTCAAAGCA TCTCCCATAG CAAATGATGG AGAAGAGGAG
    TTTGTCCCCT CAGATGGCCT AGATAAAGAT GAATACACAT TCTCACCAGG CAAATCGAAA
    GCTGCTCCAG AAAAGTCTTC CCATGACAAG AAGAGTCAGG ATTTTGGGAC TCTCTTCTCT
    TTTCCTTCAT ACTCTCAGAA GTCAGAAGAT GATTCAGCTA AATTTGACAG TAATGAAGAA
    GATTCTGCTT CTGTATTTTC ACCATCATTT GGTCTGAAGC AAACAGAAAA AGTTCCAAGT
    AAAACAGTAG CTGCTAAAAA GGGAAAACCG TCTTTAGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45376
    Clone ID Related Accession (Same CDS sequence) XM_010820383.1 , XM_015469766.1
    Accession Version XM_010820383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818685.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_010820383.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45376
    Clone ID Related Accession (Same CDS sequence) XM_010820383.1 , XM_015469766.1
    Accession Version XM_015469766.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325252.1
    CDS16..4797
    Translation

    Target ORF information:

    RefSeq Version XM_015469766.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAAAAG TCTTCCCATG ACAAGAAGAG TCAGGATTTT
    GGGACTCTCT TCTCTTTTCC TTCATACTCT CAGAAGTCAG AAGATGATTC AGCTAAATTT
    GACAGTAATG AAGAAGATTC TGCTTCTGTA TTTTCACCAT CATTTGGTCT GAAGCAAACA
    GAAAAAGTTC CAAGTAAAAC AGTAGCTGCT AAAAAGGATA CAGCCTCTAA GCCCAAGAGG
    GCCCCCAAAC AGAAGAAAGT AGAGACTGTG AACTCTGACT CGGATTCTGA ATTCGGCGTT
    CCAAAGAAAA CAGCACCGAA AGGTAAAGGC CGAGGGGCAA AGAAAAGGAA AGCATCTGGC
    TCTGAAAACG AAGGTGATTA CAATCCTGGC AGGAAAACCT CCAAACCACC AAGCAAGAAG
    CCGAAGAAAA CCTCTTTTGA TGAGGACTCA GATGTGGACC TCTTCCCCTC AGACCTCACC
    ACCGAGCCAC CGTCACTGCC GCGGACTGGC CGGGCAAGGA AAGAAGTAAA ATATTTTGCA
    GACTCTGACG AGGAAGATGT CGATTTTGCG ATGTTTAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45377
    Clone ID Related Accession (Same CDS sequence) XM_015469768.1 , XM_010820385.2
    Accession Version XM_015469768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015325254.1
    CDS16..4251
    Translation

    Target ORF information:

    RefSeq Version XM_015469768.1
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015469768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45377
    Clone ID Related Accession (Same CDS sequence) XM_015469768.1 , XM_010820385.2
    Accession Version XM_010820385.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4236bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA topoisomerase 2-beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104613.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818687.1
    CDS16..4251
    Translation

    Target ORF information:

    RefSeq Version XM_010820385.2
    Organism Bos taurus(cattle)
    Definition Bos taurus topoisomerase (DNA) II beta (TOP2B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010820385.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACTCTCT TTGATCAGAA CAATGCTGCA AAAAAGGAAG AGTCAGAAAT TGCAAACAAA 
    AATGATTCTT CAAAGAAGTT GTCCGTCGAG AGAGTGTATC AGAAAAAGAC ACAACTCGAA
    CATATTCTGC TTCGTCCTGA TACGTACATT GGGTCAGTGG AGCCATTGAC ACAGCTCATG
    TGGGTATATG ATGAAGATGT AGGAATGAAT TGCAGAGAGG TTACCTTTGT GCCGGGTTTA
    TATAAGATCT TTGATGAAAT TTTGGTTAAT GCAGCTGATA ATAAACAGAG GGATAAGAAC
    ATGACCTGTA TTAAAGTTTC TATTGATCCT GAATCTAACA TAATAAGCAT TTGGAATAAT
    GGGAAAGGCA TTCCAGTAGT AGAACACAAA GTAGAGAAAG TTTATGTTCC TGCATTAATT
    TTTGGACAGC TTTTAACGTC CAGTAACTAT GACGATGATG AGAAAAAAGT AACAGGTGGT
    CGCAATGGTT ATGGTGCAAA ACTTTGTAAT ATTTTCAGTA CAAAGTTTAC AGTAGAAACT
    GCTTGCAAAG AATACAAACA TAGTTTTAAG CAGACATGGA TGAATAATAT GATGAAGACT
    TCTGAAGCCA AAATTAAACA TTTTGATGGT GAAGATTACA CATGTATAAC ATTCCAACCA
    GATCTGTCCA AATTTAAGAT GGAGAAGCTT GACAGGGATA TTGTTGCTCT CATGACCAGA
    AGAGCTTATG ATTTGGCTGG TTCCTGCAAG GGAGTCAAGG TCATGTTTAA TGGAAAGAAA
    TTGCCGGTAA ATGGATTTCG CAGTTATGTA GATCTTTATG TGAAAGACAA ATTGGATGAA
    ACGGGAGTGG CTCTAAAAGT TATTCATGAA CTTGCAAATG AAAGATGGGA CGTTTGTCTC
    ACTTTGAGTG AAAAAGGATT CCAGCAAATC AGCTTTGTAA ATAGCATTGC AACAACAAAA
    GGTGGACGGC ATGTGGATTA CGTAGTAGAC CAAGTTGTTG GTAAACTGAT TGAAGTAGTT
    AAGAAAAAGA ACAAAGCTGG TGTGTCAGTG AAACCATTTC AAGTAAAAAA TCATATATGG
    GTTTTTATTA ACTGCCTTAT TGAAAATCCA ACTTTTGATT CTCAAACTAA GGAAAACATG
    ACTTTGCAGC CCAAAAGTTT TGGATCCAAA TGCCAACTAT CAGAAAAATT TTTTAAAGCA
    GCCTCTAACT GTGGCATTGT AGAAAGTATC CTGAACTGGG TGAAATTTAA GGCTCAGACA
    CAGCTGAACA AGAAGTGTTC ATCAGTAAAG TACAGTAAAA TCAAAGGTAT TCCCAAATTG
    GATGATGCTA ATGATGCTGG TGGCAAACAT TCCCTGGAGT GCACACTGAT CCTGACAGAA
    GGAGACTCTG CCAAGTCCCT GGCCGTGTCT GGATTAGGCG TCATTGGGAG GGACAGATAT
    GGGGTCTTTC CCCTCAGGGG AAAGATTCTT AACGTGCGGG AAGCTTCTCA CAAACAGATC
    ATGGAAAATG CAGAAATAAA CAATATTATT AAAATAGTTG GTCTACAATA TAAGAAAAGT
    TATGATGATG CAGAATCTCT GAAAACTTTA CGCTATGGAA AGATTATGAT TATGACTGAT
    CAGGATCAAG ATGGTTCTCA CATAAAAGGC CTGCTTATTA ATTTCATCCA TCACAATTGG
    CCATCACTTT TGAAGCATGG TTTTCTTGAA GAGTTCATTA CCCCTATTGT AAAGGCTAGC
    AAAAATAAGC AGGAACTTTC CTTCTACAGT ATTCCTGAAT TTGATGAGTG GAAAAAACAT
    ATAGAAAACC AGAAAGCCTG GAAAATAAAA TACTACAAAG GGTTGGGTAC CAGTACAGCT
    AAGGAAGCCA AGGAATATTT TGCTGATATG GAAAGGCACC GTATCTTATT TAGATATGCT
    GGTCCTGAAG ATGATGCTGC CATTACCTTG GCATTCAGTA AGAAGAAGAT CGATGACAGA
    AAAGAATGGT TAACGAACTT TATGGAAGAT CGGAGACAGC GTAGGTTACA TGGCTTACCA
    GAGCAATTCT TATATGGTAC AGCAACCAAG CATTTGACTT ATAATGATTT TATCAACAAG
    GAATTGATTC TCTTCTCAAA CTCAGACAAT GAAAGATCTA TACCATCTCT TGTTGATGGT
    TTTAAACCAG GTCAACGAAA AGTTTTATTT ACCTGTTTCA AGAGGAATGA TAAACGTGAA
    GTGAAAGTTG CACAGTTGGC TGGATCTGTT GCCGAGATGT CTGCTTATCA TCATGGAGAA
    CAAGCATTGA TGATGACTAT TGTGAACTTG GCTCAGAACT TTGTGGGGAG CAACAACATC
    AACTTGCTTC AGCCTATTGG TCAGTTTGGA ACTCGACTTC ACGGGGGCAA AGATGCTGCG
    AGCCCTCGTT ATATTTTCAC AATGTTGAGC ACTTTAGCAA GACTGCTTTT TCCTGCTGTG
    GATGACAACC TGCTTAAATT CCTTTATGAT GATAATCAGC GTGTGGAGCC TGAGTGGTAT
    ATTCCCATAA TTCCAATGGT TTTAATAAAT GGTGCTGAGG GCATTGGCAC AGGGTGGGCT
    TGTAAGCTGC CCAACTACGA TGCCAGAGAG ATTGTGAACA ATGTCAGGCG AATGCTCGAT
    GGCCTGGATC CTCATCCCAT GCTTCCAAAC TACAAAAACT TTAAAGGAAC GATCCAAGAA
    CTTGGTCAAA ACCAGTATGC AGTCAGTGGT GAGATATTTG TGGTGGATAG AAACACGGTG
    GAGATTACCG AGCTCCCCGT CAGAACTTGG ACACAGGTAT ACAAAGAACA GGTTTTAGAA
    CCTATGCTAA ATGGAACAGA TAAAACACCA GCATTAATTT CTGATTATAA AGAATATCAT
    ACTGATACAA CTGTGAAATT TGTAGTGAAA ATGACTGAAG AGAAGCTAGC ACAAGCAGAA
    GCTGCTGGAT TGCACAAAGT TTTTAAACTT CAAACTACTC TTACTTGTAA TTCCATGGTA
    CTCTTTGATC ATATGGGATG TCTGAAGAAG TATGAAACTG TGCAAGATAT TTTGAAAGAA
    TTCTTTGATT TACGATTAAG CTATTATGGT TTACGAAAGG AGTGGCTTGT AGGAATGTTG
    GGAGCAGAAT CTACTAAGCT TAATAATCAA GCCCGTTTCA TTTTAGAGAA GATACAAGGG
    AAAATCACTA TAGAGAATAG GTCAAAGAAA GATTTGATTC AAATGTTAGT CCAGAGAGGT
    TATGAATCTG ACCCAGTGAA AGCCTGGAAA GAAGCACAAG AAAAGGCAGC AGAAGAGGAA
    GAATCACAAA ACCAGCATGA TGATAGTTCC TCTGATTCAG GAACTCCTTC AGGCCCTGAT
    TTTAATTATA TTTTAAATAT GTCTCTATGG TCTCTTACTA AAGAAAAAGT TGAAGAACTG
    ATTAAACAGA GAGATGCAAA AGGGCGAGAG GTCAGTGATC TTAAAAGGAA ATCTCCTTCA
    GATCTTTGGA AAGAAGATTT AGCAGCTTTT GTTGAAGAAC TGGATAAAGT GGAAGCGCAG
    GAACGAGAAG ACGTTCTGGC CGGGATGGCC GGAAAAGCAA TCAAGGGGAA GGTTGGCAAA
    CCTAAGGTGA AGAAGCTTCA GCTAGAAGAG ACGATGCCCT CCCCTTACGG CAGGAGAGTG
    GTTCCTGAGA TCACTGCCAT GAAGGCAGAT GCCAGCAAGA AGCTGCTGAA GAAGAGAAAG
    GGCGATCTGG ATGCCACAGC AGTCCGAGTG GACTTTGATG AAGAGCTCAG CGGAGCAGCA
    GTGGAAGGTG GGGAAGAGGC GCCGATCCCG TCAGCTCCTG TACACAGAGG CCCCAAACCC
    AAAAGGGAGA AAAAAGAGCC TGGTACCAGG GTGAGAAAAA CACCTACATC ATCTGGTAAA
    CCGAGTGCAA AGAAAGTGAA GAAACGGAAT CCTTGGTCAG ATGATGAATC CAAGTCAGAA
    AGTGACTTGG AAGAAACAGA ACCTGTGGTT ATTCCAAGAG ACTCTTTACT TAGGAGAGCG
    GCTGCTGAAC GGCCAAAGTA TACATTCGAT TTCTCGGAAG AGGAGGAAGA TGACGCCGAT
    GATGATGACG ACAATAATGA CTTAGAGGAG CTGAAGGTCA AAGCATCTCC CATAGCAAAT
    GATGGAGAAG AGGAGTTTGT CCCCTCAGAT GGCCTAGATA AAGATGAATA CACATTCTCA
    CCAGGCAAAT CGAAAGCTGC TCCAGAATTC AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.