TOP2B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP2B
Entrez Gene ID	395966
Full Name	DNA topoisomerase 2-beta
General protein information	Preferred Names DNA topoisomerase 2-beta Names DNA topoisomerase 2-beta DNA topoisomerase II, beta isozyme DNA topoisomerase II_beta topoisomerase (DNA) II beta 180kDa topoisomerase II beta-2
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015281300.1	XP_015136786.1	DNA topoisomerase 2-beta isoform X1
XM_015281300.2	XP_015136786.1	DNA topoisomerase 2-beta isoform X1
XM_015281301.2	XP_015136787.1	DNA topoisomerase 2-beta isoform X2
XM_015281302.2	XP_015136788.1	DNA topoisomerase 2-beta isoform X3
NM_205082.1	NP_990413.1	DNA topoisomerase 2-beta
XM_015281301.1	XP_015136787.1	DNA topoisomerase 2-beta isoform X2
XM_015281302.1	XP_015136788.1	DNA topoisomerase 2-beta isoform X3

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.
Sapetto-Rebow B, McLoughlin SC, O'Shea LC, O'Leary O, Willer JR, Alvarez Y, Collery R, O'Sullivan J, Van Eeden F, Hensey C, Kennedy BN
BMC developmental biology1171(2011 Nov)

Co-localization of chicken DNA topoisomerase IIalpha, but not beta, with sites of DNA replication and possible involvement of a C-terminal region of alpha through its binding to PCNA.
Niimi A, Suka N, Harata M, Kikuchi A, Mizuno S
Chromosoma110(2)102-14(2001 May)

Two differentially spliced forms of topoisomerase IIalpha and beta mRNAs are conserved between birds and humans.
Petruti-Mot AS, Earnshaw WC
Gene258(1-2)183-92(2000 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TOP2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa145745	XM_015281300.2 Latest version!	Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa145746	XM_015281301.2 Latest version!	Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa145747	XM_015281302.2 Latest version!	Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa31490	XM_015281300.1 Latest version!	Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa31491	XM_015281301.1 Latest version!	Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa31492	XM_015281302.1 Latest version!	Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa27934	NM_205082.1 Latest version!	Gallus gallus DNA topoisomerase 2-beta (TOP2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP2B ( XM_015281300.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136786.1 Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa145745
Clone ID Related Accession (Same CDS sequence)	XM_015281300.2
Accession Version	XM_015281300.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGA TACAACAAAA TTGGACAGTG ATGAGGAGGA TTCTACTCCT 
GTTTTCTCAT CACCTTTCGC CCCAAAACAA ACAGAGAAAA TGCTAAGTAA AACAGTAGCT 
GCTAAAAAAG CAAAAGTAGA TGTACCACCT AAACCTAAAA GGGCTCCCAA GGCCAAGAAG 
ATGGAAACTG TAAATTCTGA CTCGGATTCA GAATTCGGCA TTCCAAAGAA GACTGCAGCA 
CCCAAAGGAA AAGGGAGAGG GGCAAAGAAA AGGAAAACAT CTGGCTCAGA AAATGAAGGT 
GAATACAACC CTGGGAAGAA GGCACCTAAA TCAACACCAT GCAAGAAATC CAAGAAGGCC 
GCTTTTGACC AGGATTCTGA TGTGGAAATC TTCCAGTCAG GCTTTGCCTC CGAAACTGCC 
CCGAAGCCCC GGACAGGCCG GGCTAGAAAA GAAGTGAAAT ATTTTGCAGA GTCTGATGAA 
GATGATGATT TTGATATGTT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136786.1
CDS	56..4942
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTWVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMEKLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAAERPKYTFDFSEEEDDADDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPDRKMSQEKKNQDFGNIFSFPSYSQKTDDDTTKLDSDEEDSTPVFSSPFAPKQTEKMLSKTVAAKKAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	XM_015281300.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281300.2

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGA TACAACAAAA TTGGACAGTG ATGAGGAGGA TTCTACTCCT 
GTTTTCTCAT CACCTTTCGC CCCAAAACAA ACAGAGAAAA TGCTAAGTAA AACAGTAGCT 
GCTAAAAAAG CAAAAGTAGA TGTACCACCT AAACCTAAAA GGGCTCCCAA GGCCAAGAAG 
ATGGAAACTG TAAATTCTGA CTCGGATTCA GAATTCGGCA TTCCAAAGAA GACTGCAGCA 
CCCAAAGGAA AAGGGAGAGG GGCAAAGAAA AGGAAAACAT CTGGCTCAGA AAATGAAGGT 
GAATACAACC CTGGGAAGAA GGCACCTAAA TCAACACCAT GCAAGAAATC CAAGAAGGCC 
GCTTTTGACC AGGATTCTGA TGTGGAAATC TTCCAGTCAG GCTTTGCCTC CGAAACTGCC 
CCGAAGCCCC GGACAGGCCG GGCTAGAAAA GAAGTGAAAT ATTTTGCAGA GTCTGATGAA 
GATGATGATT TTGATATGTT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_015281301.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136787.1 Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa145746
Clone ID Related Accession (Same CDS sequence)	XM_015281301.2
Accession Version	XM_015281301.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281301.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGC AAAAGTAGAT GTACCACCTA AACCTAAAAG GGCTCCCAAG 
GCCAAGAAGA TGGAAACTGT AAATTCTGAC TCGGATTCAG AATTCGGCAT TCCAAAGAAG 
ACTGCAGCAC CCAAAGGAAA AGGGAGAGGG GCAAAGAAAA GGAAAACATC TGGCTCAGAA 
AATGAAGGTG AATACAACCC TGGGAAGAAG GCACCTAAAT CAACACCATG CAAGAAATCC 
AAGAAGGCCG CTTTTGACCA GGATTCTGAT GTGGAAATCT TCCAGTCAGG CTTTGCCTCC 
GAAACTGCCC CGAAGCCCCG GACAGGCCGG GCTAGAAAAG AAGTGAAATA TTTTGCAGAG 
TCTGATGAAG ATGATGATTT TGATATGTTT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136787.1
CDS	56..4831
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTWVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMEKLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAAERPKYTFDFSEEEDDADDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPDRKMSQEKKNQDFGNIFSFPSYSQKTDDAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	XM_015281301.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281301.2

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGC AAAAGTAGAT GTACCACCTA AACCTAAAAG GGCTCCCAAG 
GCCAAGAAGA TGGAAACTGT AAATTCTGAC TCGGATTCAG AATTCGGCAT TCCAAAGAAG 
ACTGCAGCAC CCAAAGGAAA AGGGAGAGGG GCAAAGAAAA GGAAAACATC TGGCTCAGAA 
AATGAAGGTG AATACAACCC TGGGAAGAAG GCACCTAAAT CAACACCATG CAAGAAATCC 
AAGAAGGCCG CTTTTGACCA GGATTCTGAT GTGGAAATCT TCCAGTCAGG CTTTGCCTCC 
GAAACTGCCC CGAAGCCCCG GACAGGCCGG GCTAGAAAAG AAGTGAAATA TTTTGCAGAG 
TCTGATGAAG ATGATGATTT TGATATGTTT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_015281302.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136788.1 Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa145747
Clone ID Related Accession (Same CDS sequence)	XM_015281302.2
Accession Version	XM_015281302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015281302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGAAAAA 
ATGTCTCAAG AGAAGAAAAA TCAAGACTTT GGGAACATTT TCTCCTTCCC ATCTTACTCT 
CAGAAGACAG ATGATGCAAA AGTAGATGTA CCACCTAAAC CTAAAAGGGC TCCCAAGGCC 
AAGAAGATGG AAACTGTAAA TTCTGACTCG GATTCAGAAT TCGGCATTCC AAAGAAGACT 
GCAGCACCCA AAGGAAAAGG GAGAGGGGCA AAGAAAAGGA AAACATCTGG CTCAGAAAAT 
GAAGGTGAAT ACAACCCTGG GAAGAAGGCA CCTAAATCAA CACCATGCAA GAAATCCAAG 
AAGGCCGCTT TTGACCAGGA TTCTGATGTG GAAATCTTCC AGTCAGGCTT TGCCTCCGAA 
ACTGCCCCGA AGCCCCGGAC AGGCCGGGCT AGAAAAGAAG TGAAATATTT TGCAGAGTCT 
GATGAAGATG ATGATTTTGA TATGTTTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136788.1
CDS	56..4828
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTWVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMEKLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAAERPKYTFDFSEEEDDADDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPEKMSQEKKNQDFGNIFSFPSYSQKTDDAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	XM_015281302.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA topoisomerase 2-beta (TOP2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281302.2

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGAAAAA 
ATGTCTCAAG AGAAGAAAAA TCAAGACTTT GGGAACATTT TCTCCTTCCC ATCTTACTCT 
CAGAAGACAG ATGATGCAAA AGTAGATGTA CCACCTAAAC CTAAAAGGGC TCCCAAGGCC 
AAGAAGATGG AAACTGTAAA TTCTGACTCG GATTCAGAAT TCGGCATTCC AAAGAAGACT 
GCAGCACCCA AAGGAAAAGG GAGAGGGGCA AAGAAAAGGA AAACATCTGG CTCAGAAAAT 
GAAGGTGAAT ACAACCCTGG GAAGAAGGCA CCTAAATCAA CACCATGCAA GAAATCCAAG 
AAGGCCGCTT TTGACCAGGA TTCTGATGTG GAAATCTTCC AGTCAGGCTT TGCCTCCGAA 
ACTGCCCCGA AGCCCCGGAC AGGCCGGGCT AGAAAAGAAG TGAAATATTT TGCAGAGTCT 
GATGAAGATG ATGATTTTGA TATGTTTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_015281300.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136786.1 Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X1, mRNA.

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CloneID	OGa31490
Clone ID Related Accession (Same CDS sequence)	XM_015281300.1
Accession Version	XM_015281300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGAGGA AAGTTCTGTT CACGTGTTTC 
AAAAGAAACG ACAAACGGGA GGTGAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGA TACAACAAAA TTGGACAGTG ATGAGGAGGA TTCTACTCCT 
GTTTTCTCAT CACCTTTCGC CCCAAAACAA ACAGAGAAAA TGCTAAGTAA AACAGTAGCT 
GCTAAAAAAG CAAAAGTAGA TGTACCACCT AAACCTAAAA GGGCTCCCAA GGCCAAGAAG 
ATGGAAACTG TAAATTCTGA CTCGGATTCA GAATTCGGCA TTCCAAAGAA GACTGCAGCA 
CCCAAAGGAA AAGGGAGAGG GGCAAAGAAA AGGAAAACAT CTGGCTCAGA AAATGAAGGT 
GAATACAACC CTGGGAAGAA GGCACCTAAA TCAACACCAT GCAAGAAATC CAAGAAGGCC 
GCTTTTGACC AGGATTCTGA TGTGGAAATC TTCCAGTCAG GCTTTGCCTC CGAAACTGCC 
CCGAAGCCCC GGACAGGCCG GGCTAGAAAA GAAGTGAAAT ATTTTGCAGA GTCTGATGAA 
GATGATGATT TTGATATGTT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136786.1
CDS	24..4910
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTWVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMEKLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAAERPKYTFDFSEEEDDADDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPDRKMSQEKKNQDFGNIFSFPSYSQKTDDDTTKLDSDEEDSTPVFSSPFAPKQTEKMLSKTVAAKKAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	XM_015281300.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281300.1

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGAGGA AAGTTCTGTT CACGTGTTTC 
AAAAGAAACG ACAAACGGGA GGTGAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGA TACAACAAAA TTGGACAGTG ATGAGGAGGA TTCTACTCCT 
GTTTTCTCAT CACCTTTCGC CCCAAAACAA ACAGAGAAAA TGCTAAGTAA AACAGTAGCT 
GCTAAAAAAG CAAAAGTAGA TGTACCACCT AAACCTAAAA GGGCTCCCAA GGCCAAGAAG 
ATGGAAACTG TAAATTCTGA CTCGGATTCA GAATTCGGCA TTCCAAAGAA GACTGCAGCA 
CCCAAAGGAA AAGGGAGAGG GGCAAAGAAA AGGAAAACAT CTGGCTCAGA AAATGAAGGT 
GAATACAACC CTGGGAAGAA GGCACCTAAA TCAACACCAT GCAAGAAATC CAAGAAGGCC 
GCTTTTGACC AGGATTCTGA TGTGGAAATC TTCCAGTCAG GCTTTGCCTC CGAAACTGCC 
CCGAAGCCCC GGACAGGCCG GGCTAGAAAA GAAGTGAAAT ATTTTGCAGA GTCTGATGAA 
GATGATGATT TTGATATGTT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_015281301.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136787.1 Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X2, mRNA.

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CloneID	OGa31491
Clone ID Related Accession (Same CDS sequence)	XM_015281301.1
Accession Version	XM_015281301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGAGGA AAGTTCTGTT CACGTGTTTC 
AAAAGAAACG ACAAACGGGA GGTGAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGC AAAAGTAGAT GTACCACCTA AACCTAAAAG GGCTCCCAAG 
GCCAAGAAGA TGGAAACTGT AAATTCTGAC TCGGATTCAG AATTCGGCAT TCCAAAGAAG 
ACTGCAGCAC CCAAAGGAAA AGGGAGAGGG GCAAAGAAAA GGAAAACATC TGGCTCAGAA 
AATGAAGGTG AATACAACCC TGGGAAGAAG GCACCTAAAT CAACACCATG CAAGAAATCC 
AAGAAGGCCG CTTTTGACCA GGATTCTGAT GTGGAAATCT TCCAGTCAGG CTTTGCCTCC 
GAAACTGCCC CGAAGCCCCG GACAGGCCGG GCTAGAAAAG AAGTGAAATA TTTTGCAGAG 
TCTGATGAAG ATGATGATTT TGATATGTTT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136787.1
CDS	24..4799
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTWVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMEKLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAAERPKYTFDFSEEEDDADDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPDRKMSQEKKNQDFGNIFSFPSYSQKTDDAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	XM_015281301.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281301.1

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGAGGA AAGTTCTGTT CACGTGTTTC 
AAAAGAAACG ACAAACGGGA GGTGAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGATAGA 
AAAATGTCTC AAGAGAAGAA AAATCAAGAC TTTGGGAACA TTTTCTCCTT CCCATCTTAC 
TCTCAGAAGA CAGATGATGC AAAAGTAGAT GTACCACCTA AACCTAAAAG GGCTCCCAAG 
GCCAAGAAGA TGGAAACTGT AAATTCTGAC TCGGATTCAG AATTCGGCAT TCCAAAGAAG 
ACTGCAGCAC CCAAAGGAAA AGGGAGAGGG GCAAAGAAAA GGAAAACATC TGGCTCAGAA 
AATGAAGGTG AATACAACCC TGGGAAGAAG GCACCTAAAT CAACACCATG CAAGAAATCC 
AAGAAGGCCG CTTTTGACCA GGATTCTGAT GTGGAAATCT TCCAGTCAGG CTTTGCCTCC 
GAAACTGCCC CGAAGCCCCG GACAGGCCGG GCTAGAAAAG AAGTGAAATA TTTTGCAGAG 
TCTGATGAAG ATGATGATTT TGATATGTTT AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( XM_015281302.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015136788.1 Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X3, mRNA.

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CloneID	OGa31492
Clone ID Related Accession (Same CDS sequence)	XM_015281302.1
Accession Version	XM_015281302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455773.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGAGGA AAGTTCTGTT CACGTGTTTC 
AAAAGAAACG ACAAACGGGA GGTGAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGAAAAA 
ATGTCTCAAG AGAAGAAAAA TCAAGACTTT GGGAACATTT TCTCCTTCCC ATCTTACTCT 
CAGAAGACAG ATGATGCAAA AGTAGATGTA CCACCTAAAC CTAAAAGGGC TCCCAAGGCC 
AAGAAGATGG AAACTGTAAA TTCTGACTCG GATTCAGAAT TCGGCATTCC AAAGAAGACT 
GCAGCACCCA AAGGAAAAGG GAGAGGGGCA AAGAAAAGGA AAACATCTGG CTCAGAAAAT 
GAAGGTGAAT ACAACCCTGG GAAGAAGGCA CCTAAATCAA CACCATGCAA GAAATCCAAG 
AAGGCCGCTT TTGACCAGGA TTCTGATGTG GAAATCTTCC AGTCAGGCTT TGCCTCCGAA 
ACTGCCCCGA AGCCCCGGAC AGGCCGGGCT AGAAAAGAAG TGAAATATTT TGCAGAGTCT 
GATGAAGATG ATGATTTTGA TATGTTTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015136788.1
CDS	24..4796
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTWVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMEKLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAAERPKYTFDFSEEEDDADDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPEKMSQEKKNQDFGNIFSFPSYSQKTDDAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	XM_015281302.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus topoisomerase (DNA) II beta 180kDa (TOP2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015281302.1

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGGGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GCGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTAAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA CGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAAACT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AACGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATTCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGAGGA AAGTTCTGTT CACGTGTTTC 
AAAAGAAACG ACAAACGGGA GGTGAAGGTT GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GAGAGGCCCA AGTATACATT TGACTTCTCA 
GAAGAAGAAG ATGATGCCGA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GACAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGAAAAA 
ATGTCTCAAG AGAAGAAAAA TCAAGACTTT GGGAACATTT TCTCCTTCCC ATCTTACTCT 
CAGAAGACAG ATGATGCAAA AGTAGATGTA CCACCTAAAC CTAAAAGGGC TCCCAAGGCC 
AAGAAGATGG AAACTGTAAA TTCTGACTCG GATTCAGAAT TCGGCATTCC AAAGAAGACT 
GCAGCACCCA AAGGAAAAGG GAGAGGGGCA AAGAAAAGGA AAACATCTGG CTCAGAAAAT 
GAAGGTGAAT ACAACCCTGG GAAGAAGGCA CCTAAATCAA CACCATGCAA GAAATCCAAG 
AAGGCCGCTT TTGACCAGGA TTCTGATGTG GAAATCTTCC AGTCAGGCTT TGCCTCCGAA 
ACTGCCCCGA AGCCCCGGAC AGGCCGGGCT AGAAAAGAAG TGAAATATTT TGCAGAGTCT 
GATGAAGATG ATGATTTTGA TATGTTTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOP2B ( NM_205082.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_990413.1 Gallus gallus DNA topoisomerase 2-beta (TOP2B), mRNA.

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CloneID	OGa27934
Clone ID Related Accession (Same CDS sequence)	NM_205082.1
Accession Version	NM_205082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Gallus gallus(chicken)
Product	DNA topoisomerase 2-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB007446.1. ##Evidence-Data-START## Transcript exon combination :: AB007446.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGTGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GAGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTGAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA TGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAACCT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AATGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATCCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTG GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GACAGGGCCA AGTATACATT TGACTTCTCA 
AAAGAAGAGG ATGATGCCCA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GATAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGAAAAA 
ATGTCTCAAG AGAAGAAAAA TCAAGACTTT GGGAACATTT TCTCATTCCC ATCTTACTCT 
CAGAAGACAG ACGATGATAC AACAAAATTG GACAGTGATG AGGAGGATTC TACTCCTGTT 
TTCTCATCAC CTTTCGCCCC AAAACAAACA GAGAAAATGC TAAGTAAAAC AGTAGCTGCT 
AAAAAAGCAA AAGTAGATGT ACCACCTAAA CCTAAAAGGG CTCCCAAGGC CAAGAAGATG 
GAAACTGTAA ATTCTGACTC GGATTCAGAA TTCGGCATTC CAAAGAAGAC TGCAGCACCC 
AAAGGAAAAG GGAGAGGGGC AAAGAAAAGG AAAACATCTG GCTCAGAAAA TGAAGGTGAA 
TACAACCCTG GGAAGAAGGC ACCTAAATCA ACACCATGCA AGAAATCCAA GAAGGCCGCT 
TTTGACCAGG ATTCTGATGT GGAAATCTTC CAGTCAGGCT TTGCCTCCGA AACTGCCCCG 
AAGCCCCGGA CAGGCCGGGC TAGAAAAGAA GTGAAATATT TTGCAGAGTC TGATGAAGAT 
GATGATTTTG ATATGTTTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_990413.1
CDS	203..5086
Translation	MAKSGGGGGGGGGGGGGGGGSGGLTCVTLFDNQINASKKEESESVNKNDTSKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMENLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESDLEESEPVIIPRDSLLRRAAADRAKYTFDFSKEEDDAHDDDDANNNNDLDELKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPEKMSQEKKNQDFGNIFSFPSYSQKTDDDTTKLDSDEEDSTPVFSSPFAPKQTEKMLSKTVAAKKAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKRKTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAPKPRTGRARKEVKYFAESDEDDDFDMFN

Target ORF information:

RefSeq Version	NM_205082.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus DNA topoisomerase 2-beta (TOP2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205082.1

ORF Insert Sequence:

ATGGCAAAGT CCGGAGGAGG AGGCGGCGGC GGCGGTGGTG GCGGCGGCGG TGGTGGTGGC 
AGCGGGGGAC TGACCTGTGT GACATTATTT GATAATCAAA TCAATGCCTC AAAAAAGGAA 
GAATCAGAAA GTGTTAATAA AAATGACACT TCAAAGAAGA TGTCTGTTGA GAGAGTTTAT 
CAGAAAAAGA CTCAGCTTGA ACATATCCTC CTCCGTCCAG ACACCTATAT TGGATCAGTT 
GAACCTTTAA CTCAGTTAAT GTGGGTTTAT GATGAAGATG TCGGAATGAA TTGCAGAGAG 
GTTACCTTTG TCCCTGGCTT ATACAAGATC TTTGATGAAA TCCTTGTGAA TGCCGCTGAC 
AATAAGCAGA GGGACAAGAA TATGACCTGT ATTAAAATAT CCATTGATCC AGAGTCAAAC 
ATTATCAGCA TATGGAATAA TGGAAAGGGT ATACCAGTTG TGGAACACAA AGTAGAAAAA 
GTGTATGTTC CTGCTTTAAT CTTTGGGCAG CTGTTAACAT CTAGCAACTA CGATGATGAT 
GAGAAAAAAG TTACAGGTGG TCGTAATGGC TACGGCGCAA AACTCTGTAA CATATTTAGT 
ACAAAGTTTA CAGTTGAAAC TGCTTGCAAG GAATACAAAC ACAGCTTTAA GCAGACTTGG 
ATGAACAATA TGATGAAGAC TTCTGAACCC AAGATTAAGC ATTTTGAAGG TGACGACTAC 
ACATGCATTA CATTCCAACC AGATCTATCT AAATTTAAAA TGGAAAACCT TGACAAGGAT 
ATTGTGTCCC TCATGACAAG AAGAGCATAT GATTTGGCTG GGTCGTGCAA AGGAGTCAAA 
GTTATGTTGA ATGGGAAGAA GTTACCTGTA AATGGATTTC GCAGTTACGT AGATCTTTAC 
GTAAAGGACA AGCTGGATGA AACTGGAGTT GCACTCAAAG TTATTCATGA AGTCGTTAAT 
GAACGATGGG ATGTGTGCCT AACTTTAAGT GAAAAAGGAT TTCAGCAAAT CAGCTTTGTA 
AATAGTATAG CTACCACAAA GGGTGGTAGG CATGTTGATT ACGTGGTGGA TCAGGTTGTG 
GGCAAACTGA TAGAAGTGGT AAAAAAGAAG AATAAAGCTG GAGTTTCAGT GAAACCATTT 
CAGGTGAAGA ATCATATATG GGTTTTTGTT AATTGCTTGA TTGAAAACCC ATCTTTTGAT 
TCCCAGACAA AGGAAAACAT GACATTGCAG CCTAAAAGTT TTGGGTCCAA GTGCCAGCTA 
TCCGAGAAAT TTTTTAAAGC AGCCTCCAAC TGTGGTATCA TAGAGAGTAT TTTGAATTGG 
GTCAAATTTA AGGCGCAGAC TCAACTGAAC AAAAAATGCT CATCTGTGAA ACACAGTAAA 
ATCAAAGGCA TTCCAAAACT GGATGATGCT AATGATGCAG GTGGCAAACA TTCCTTGGAC 
TGCACACTAA TACTAACAGA AGGAGACTCT GCCAAATCTT TAGCTGTCTC TGGCTTAGGT 
GTTATTGGTC GGGACAGATA CGGAGTATTC CCACTCAGGG GTAAAATTCT CAATGTTCGA 
GAAGCTTCTC ACAAGCAGAT AATGGAAAAT GCAGAAATCA ACAACATCAT CAAAATAGTT 
GGACTGCAAT ACAAGAAAAG CTATGAAGAT CCAGAATCTC TAAAAAGTCT GAGATATGGA 
AAAATCATGA TAATGACAGA TCAGGATCAG GATGGATCTC ACATAAAAGG CTTGTTGATC 
AATTTTATTC ATCACAATTG GCCATCACTG TTGAAACATG GTTTTCTTGA AGAATTCATC 
ACTCCTATTG TAAAGGCAAG CAAAAATAAG CAAGAACTTT CATTCTATAG TATTCCTGAA 
TTTGACGAGT GGAAGAAACA TATGGAAAAT CATAAAGCAT GGAAGATAAA ATATTATAAA 
GGTTTGGGTA CAAGCACTGC TAAAGAAGCA AAAGAATATT TTGCTGACAT GGAAAGACAT 
CGAATCTTAT TTCGATATGC TGGTCCTGAA GATGATGCTG CCATTACACT GGCCTTCAGT 
AAAAAGAAGA TTGATGATCG AAAAGAATGG TTAACAAACT TCATGGAGGA CAGGCGACAG 
CGTCGGCTAC ATGGCCTTCC TGAGCAATTT TTATATGGTA CAGCGACGAA ACATTTGACC 
TACAATGACT TCATTAACAA GGAGTTGATC CTTTTCTCAA ATTCAGACAA CGAAAGATCT 
ATCCCCTCCC TTGTTGATGG TTTAAAACCA GGGCAGCGCA AAGTTTTGTT CACTTGCTTT 
AAGAGAAATG ACAAACGTGA AGTAAAGGTG GCTCAGCTGG CTGGTTCTGT TGCTGAAATG 
TCAGCTTATC ACCACGGGGA GCAAGCATTA ATGATGACTA TTGTCAACTT GGCTCAGAAC 
TTCGTTGGAA GTAACAATGT TAATCTTCTA CAACCTATTG GTCAGTTTGG TACCAGGCTT 
CATGGTGGAA AGGATGCTGC CAGCCCTCGC TATATTTTCA CTATGCTAAG CCCTTTAGCC 
AGGCTTCTTT TTCCATCGGT GGATGACAAC TTGCTGAAAT TCCTTTATGA TGACAACCAA 
CGTGTAGAGC CTGAATGGTA TATTCCCATC ATTCCTATGG TTTTAGTAAA CGGAGCTGAA 
GGAATTGGTA CTGGATGGGC TTGCAAACTT CCAAACTACG ACACCAGAGA GATTGTAAAC 
AATGTCAGAC GAATGCTGGA TGGCTTAGAT CCCCATCCTA TGCTCCCAAA CTACAAAAAC 
TTCAGAGGAA CCATTCAAGA ACTTGGTCAA AACCAGTATG TAGTCAGTGG TGAAATATTT 
GTGGTGGACA GGAACACTGT AGAAATTACA GAGCTACCTG TAAGGACATG GACCCAGGTG 
TACAAAGAAC AGGTTCTAGA ACCCATGTTG AATGGAACTG AAAAAACTCC AGCATTAATT 
TCTGACTACA AGGAATACCA CACTGATACA ACTGTGAAAT TCGTTGTGAA GATGACAGAA 
GAAAAACTTG CACAGGCAGA AGCTGCTGGA CTGCACAAAG TCTTTAAGCT TCAAACAAGT 
CTTACTTGTA ATTCTATGGT GCTATTTGAT CATATGGGAT GTTTAAAGAA GTATGAAACT 
GTGCAAGACA TTTTGAAGGA GTTCTTTGAT TTGCGATTGC ATTATTATAG TTTACGTAAG 
GAATGGCTTG TGGGAATGTT GGGTGCTGAA TCTACAAAGC TAAACAACCA AGCTCGCTTC 
ATTCTGGAGA AGATACAAGG AAAAATTACT ATAGAGAATA GATCAAAAAG AGATTTGATT 
CAAATGTTGG TTCAGAGGGG CTATGAATCT GATCCAGTTA AAGCTTGGAA AGAAGCACAG 
GAAAAGGCAG CAGAAGAGGA GGACCCACAA AACGCAAATG ATGATGCTTC CTCTGCTTCT 
GGATCAACTT CTGGCCCTGA TTTTAACTAT ATCTTAAACA TGTCTCTGTG GTCACTTACA 
AAAGAGAAAG TGGAAGAGCT CATTAAGCAC AGAGATTCAA AGGAGAGAGA ACTAAATGAC 
CTTAAAAGGA AATCTGCTTC AGATCTCTGG AAAGAAGACT TAGCAGCTTT TGTTGAAGAG 
CTTGAAAAAG TAGAAGCACA GGAAAGAGAA GATGTTCTGG CTGGTATGGT TGGTAAACCA 
ATCAAAGGTA AAGTTGGTAA ACCCAAGATG AAGAAACTTC AGTTGGAAGA GACCATGCCA 
TCACCATTTG GTAGAAGAAT AGTTCCACAG ATTACATCTG CCATGAAAGC TGATGCCAGT 
AGGAAATTGC TGAAAAAGAA AAAGGGTGAT GCTGATTCTG TAGCAATAAA AATGGAATTT 
GATGAGGAAT TTGGTGGTGT GCAAGCCGAG GGTGGTGGGG ATGACACGGT GAATACAGCA 
GCCTCAGGAA CTAAAACCCC TAAACTGAAG AGAGAGAAGA AGGAGCCTGG TACTAGAGTA 
AGAAGAGCGC CATCATCTAC AAAATCCAGT GCAAAAAAAG TGAAGAAACG AAACCCCTGG 
TCAGATGATG AATCCAAGTC TGAAAGTGAT TTAGAAGAGA GTGAGCCTGT GATTATTCCA 
AGAGACTCTC TGCTTAGGAG AGCTGCAGCT GACAGGGCCA AGTATACATT TGACTTCTCA 
AAAGAAGAGG ATGATGCCCA TGATGATGAT GATGCCAACA ATAATAATGA CTTAGATGAG 
CTTAAAGTAA AAGCCTCTCC AGTTATAAAT GATAGAGAGG ATGAGTTCGT TCCCTCAGAC 
AGTGTAGAAA AAGATGAATA TGACTTCTCT CCAGTCAAAT CAAAGCCATC TCCAGAAAAA 
ATGTCTCAAG AGAAGAAAAA TCAAGACTTT GGGAACATTT TCTCATTCCC ATCTTACTCT 
CAGAAGACAG ACGATGATAC AACAAAATTG GACAGTGATG AGGAGGATTC TACTCCTGTT 
TTCTCATCAC CTTTCGCCCC AAAACAAACA GAGAAAATGC TAAGTAAAAC AGTAGCTGCT 
AAAAAAGCAA AAGTAGATGT ACCACCTAAA CCTAAAAGGG CTCCCAAGGC CAAGAAGATG 
GAAACTGTAA ATTCTGACTC GGATTCAGAA TTCGGCATTC CAAAGAAGAC TGCAGCACCC 
AAAGGAAAAG GGAGAGGGGC AAAGAAAAGG AAAACATCTG GCTCAGAAAA TGAAGGTGAA 
TACAACCCTG GGAAGAAGGC ACCTAAATCA ACACCATGCA AGAAATCCAA GAAGGCCGCT 
TTTGACCAGG ATTCTGATGT GGAAATCTTC CAGTCAGGCT TTGCCTCCGA AACTGCCCCG 
AAGCCCCGGA CAGGCCGGGC TAGAAAAGAA GTGAAATATT TTGCAGAGTC TGATGAAGAT 
GATGATTTTG ATATGTTTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a-/- mutants.
BMC developmental biology1171(2011 Nov)
Sapetto-Rebow B,McLoughlin SC,O'Shea LC,O'Leary O,Willer JR,Alvarez Y,Collery R,O'Sullivan J,Van Eeden F,Hensey C,Kennedy BN

Co-localization of chicken DNA topoisomerase IIalpha, but not beta, with sites of DNA replication and possible involvement of a C-terminal region of alpha through its binding to PCNA.
Chromosoma110(2)102-14(2001 May)
Niimi A,Suka N,Harata M,Kikuchi A,Mizuno S

Two differentially spliced forms of topoisomerase IIalpha and beta mRNAs are conserved between birds and humans.
Gene258(1-2)183-92(2000 Nov)
Petruti-Mot AS,Earnshaw WC

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TOP2B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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