slit2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	slit2
Entrez Gene ID	100492007
Full Name	slit guidance ligand 2
Synonyms	slil3,slit-2
General protein information	Preferred Names slit guidance ligand 2 Names slit homolog 2 protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012955707.2	XP_012811161.1	slit homolog 2 protein isoform X3
XM_031895351.1	XP_031751211.1	slit homolog 2 protein isoform X5
XM_031895349.1	XP_031751209.1	slit homolog 2 protein isoform X1
XM_012955706.2	XP_012811160.1	slit homolog 2 protein isoform X1
XM_031895350.1	XP_031751210.1	slit homolog 2 protein isoform X3
XM_002936715.4	XP_002936761.2	slit homolog 2 protein isoform X2
XM_012955706.3	XP_012811160.1	slit homolog 2 protein isoform X2
XM_002936715.5	XP_002936761.2	slit homolog 2 protein isoform X4

Pathways from BioSystems

Homologies

	SLIT2	XP_002808451.1
Canis lupus familiaris (dog)	SLIT2	XP_005618806.1
Danio rerio (zebrafish)	slit2	NP_571810.1
Xenopus tropicalis (tropical clawed frog)	slit2	XP_004911285.1
Homo sapiens (human)	SLIT2	NP_004778.1
Pan troglodytes (chimpanzee)	SLIT2	XP_003950343.1
Gallus gallus (chicken)	SLIT2	NP_001254004.1
Caenorhabditis elegans (roundworm)	slt-1	NP_510437.2
Bos taurus (cattle)	SLIT2	NP_001178445.2
Mus musculus (house mouse)	Slit2	NP_848919.3
Rattus norvegicus (Norway rat)	Slit2	NP_072154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002793	XP_312121.5
Drosophila melanogaster (fruit fly)	sli	NP_001261017.1

Gene Ontology

The following slit2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slit2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04764	XM_002936715.5 Latest version!	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa35231	XM_012955706.3 Latest version!	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa49659	XM_031895349.1 Latest version!	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa49660	XM_031895350.1 Latest version!	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa49661	XM_031895351.1 Latest version!	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa35232	XM_012955707.2 Latest version!	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OXa35231	XM_012955706.2	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa04764	XM_002936715.4	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

slit2 ( XM_002936715.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936761.2 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa04764
Clone ID Related Accession (Same CDS sequence)	XM_002936715.4 , XM_002936715.5
Accession Version	XM_002936715.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002936715.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GGTGAACTTT GTGAAGAAAA ACTGGATTTC 
TGCTCCCAAG ATGTCAATCC ATGTCAGCAT GACTCAAAAT GCATCCTGAC ACTGAAAGGC 
TACAAATGTG ACTGCACCCC CGGATATATT GGGGAACACT GTGATATCGA CTACGATGAT 
TGTCAGGAGA ACAAGTGCAG AAATGGAGCC CGTTGCGTTG ATGCAGTGAA TGGTTATACT 
TGTGTCTGCC CAGAAGGATA CAGCGGTTTG TTCTGTGAAT CGGCGCCACC AATGGTTTTA 
CCTCGCACAA GTCCTTGTGA CAACTTTGAA TGTCAAAATG GAGCCCAGTG TATCGTGAAA 
GGAAATGAGC CTCAGTGCCA GTGCTTGCCC GGATATCACG GAAACAAATG TGAAAAACTA 
GTCAGTGTAA ATTTTGTGAA CAAAGACTCG TTTATCCAAA TTCCTTCGGC AAAGATCCGC 
ACTCAGGCCA ACATCACTCT GCAGGTCGCC ACGGATGAAG ACAGTGGGAT CTTACTCTAT 
AAAGGTGACA AGGATCAGGT AGCGGTGGAG TTGTATCGTG GGAGAGTGAG AGTTAGTTAC 
GACACTGGAA TTAACCCTGC ATCTGCCATT TACAGCGTTG AGACTATAAA TGATGGAAAT 
TTCCATGTTG TGGAATTGGT AAACCTGGAT CAAACACTGT CACTGGTCGT TGATGGAGGA 
ACCCCTAAAT CCATTACGAA TTTACCAAAA CAGTCATTAA TAAGTATTGA CTCCACCCTT 
TACATAGGAG GTATGCCCAT GAAAAATAAC ATTGCAGGTC TTCGACAGTC TTCAAGTCAA 
AATGGCACCA ACTTCCTAGG CTGTATTAGG AACCTGTACA TCAATAATGA ACTACAGGAC 
TTCAGAAAAC TAAATCTCCA AGCTGGAGTT CTTCCTGGCT GTGAACCTTG CCATAAAAAA 
GTCTGTGTCC ATGGCACATG TCTGGCTACT AGCCAAGCTG GCTTCACTTG CGAATGCGAA 
GGAGGATGGA CAGGACAACT GTGTGATATC CAGTCCAACG ATCCCTGTCT GGGAAACAAG 
TGTGTCCATG GCACGTGCCT CCCCATTAAT TCATTCTCCT ACAGCTGTAA ATGCCTTCAG 
GGCTTTGTGG GGGTTCTCTG TGATGAACCT GAAGATCAGC CCAATCCCTG CCAATCCATT 
AAATGTAAAC ACGGAAAATG CAGGCTTTCG GGCTTAGGGA AAGCCTATTG TGAATGCAAC 
AGTGGTTATA CAGGGGACAG CTGTGATAAA GAAATCTCTT GCCGAGGGGA ACGGGTCAGA 
GATTACTACC AAAGGCAGCA AGGTTATGCT GCTTGCCAGA CGACCGAGAA GGTATCAAGA 
CTAGAATGCA AAGGAGGATG CTCAAATGGT CAGTGCTGTG GGCCACTGAG GAGCAAAAGG 
CGGAAATATG CTTTTGAATG CACAGATGGT TCATCCTTTG TGGACGAGGT GGAGAAAGTC 
ATAAAGTGCG GTTGTACAAA GTGCCCCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936761.2
CDS	732..5324
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKEISCRGERVRDYYQRQQGYAACQTTEKVSRLECKGGCSNGQCCGPLRSKRRKYAFECTDGSSFVDEVEKVIKCGCTKCPS

Target ORF information:

RefSeq Version	XM_002936715.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936715.5

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GGTGAACTTT GTGAAGAAAA ACTGGATTTC 
TGCTCCCAAG ATGTCAATCC ATGTCAGCAT GACTCAAAAT GCATCCTGAC ACTGAAAGGC 
TACAAATGTG ACTGCACCCC CGGATATATT GGGGAACACT GTGATATCGA CTACGATGAT 
TGTCAGGAGA ACAAGTGCAG AAATGGAGCC CGTTGCGTTG ATGCAGTGAA TGGTTATACT 
TGTGTCTGCC CAGAAGGATA CAGCGGTTTG TTCTGTGAAT CGGCGCCACC AATGGTTTTA 
CCTCGCACAA GTCCTTGTGA CAACTTTGAA TGTCAAAATG GAGCCCAGTG TATCGTGAAA 
GGAAATGAGC CTCAGTGCCA GTGCTTGCCC GGATATCACG GAAACAAATG TGAAAAACTA 
GTCAGTGTAA ATTTTGTGAA CAAAGACTCG TTTATCCAAA TTCCTTCGGC AAAGATCCGC 
ACTCAGGCCA ACATCACTCT GCAGGTCGCC ACGGATGAAG ACAGTGGGAT CTTACTCTAT 
AAAGGTGACA AGGATCAGGT AGCGGTGGAG TTGTATCGTG GGAGAGTGAG AGTTAGTTAC 
GACACTGGAA TTAACCCTGC ATCTGCCATT TACAGCGTTG AGACTATAAA TGATGGAAAT 
TTCCATGTTG TGGAATTGGT AAACCTGGAT CAAACACTGT CACTGGTCGT TGATGGAGGA 
ACCCCTAAAT CCATTACGAA TTTACCAAAA CAGTCATTAA TAAGTATTGA CTCCACCCTT 
TACATAGGAG GTATGCCCAT GAAAAATAAC ATTGCAGGTC TTCGACAGTC TTCAAGTCAA 
AATGGCACCA ACTTCCTAGG CTGTATTAGG AACCTGTACA TCAATAATGA ACTACAGGAC 
TTCAGAAAAC TAAATCTCCA AGCTGGAGTT CTTCCTGGCT GTGAACCTTG CCATAAAAAA 
GTCTGTGTCC ATGGCACATG TCTGGCTACT AGCCAAGCTG GCTTCACTTG CGAATGCGAA 
GGAGGATGGA CAGGACAACT GTGTGATATC CAGTCCAACG ATCCCTGTCT GGGAAACAAG 
TGTGTCCATG GCACGTGCCT CCCCATTAAT TCATTCTCCT ACAGCTGTAA ATGCCTTCAG 
GGCTTTGTGG GGGTTCTCTG TGATGAACCT GAAGATCAGC CCAATCCCTG CCAATCCATT 
AAATGTAAAC ACGGAAAATG CAGGCTTTCG GGCTTAGGGA AAGCCTATTG TGAATGCAAC 
AGTGGTTATA CAGGGGACAG CTGTGATAAA GAAATCTCTT GCCGAGGGGA ACGGGTCAGA 
GATTACTACC AAAGGCAGCA AGGTTATGCT GCTTGCCAGA CGACCGAGAA GGTATCAAGA 
CTAGAATGCA AAGGAGGATG CTCAAATGGT CAGTGCTGTG GGCCACTGAG GAGCAAAAGG 
CGGAAATATG CTTTTGAATG CACAGATGGT TCATCCTTTG TGGACGAGGT GGAGAAAGTC 
ATAAAGTGCG GTTGTACAAA GTGCCCCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_012955706.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811160.1 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa35231
Clone ID Related Accession (Same CDS sequence)	XM_012955706.2 , XM_012955706.3
Accession Version	XM_012955706.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012955706.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GCTGCTAAAT TGAATGAGGT GGAAAAAGGT 
GAACTTTGTG AAGAAAAACT GGATTTCTGC TCCCAAGATG TCAATCCATG TCAGCATGAC 
TCAAAATGCA TCCTGACACT GAAAGGCTAC AAATGTGACT GCACCCCCGG ATATATTGGG 
GAACACTGTG ATATCGACTA CGATGATTGT CAGGAGAACA AGTGCAGAAA TGGAGCCCGT 
TGCGTTGATG CAGTGAATGG TTATACTTGT GTCTGCCCAG AAGGATACAG CGGTTTGTTC 
TGTGAATCGG CGCCACCAAT GGTTTTACCT CGCACAAGTC CTTGTGACAA CTTTGAATGT 
CAAAATGGAG CCCAGTGTAT CGTGAAAGGA AATGAGCCTC AGTGCCAGTG CTTGCCCGGA 
TATCACGGAA ACAAATGTGA AAAACTAGTC AGTGTAAATT TTGTGAACAA AGACTCGTTT 
ATCCAAATTC CTTCGGCAAA GATCCGCACT CAGGCCAACA TCACTCTGCA GGTCGCCACG 
GATGAAGACA GTGGGATCTT ACTCTATAAA GGTGACAAGG ATCAGGTAGC GGTGGAGTTG 
TATCGTGGGA GAGTGAGAGT TAGTTACGAC ACTGGAATTA ACCCTGCATC TGCCATTTAC 
AGCGTTGAGA CTATAAATGA TGGAAATTTC CATGTTGTGG AATTGGTAAA CCTGGATCAA 
ACACTGTCAC TGGTCGTTGA TGGAGGAACC CCTAAATCCA TTACGAATTT ACCAAAACAG 
TCATTAATAA GTATTGACTC CACCCTTTAC ATAGGAGGTA TGCCCATGAA AAATAACATT 
GCAGGTCTTC GACAGTCTTC AAGTCAAAAT GGCACCAACT TCCTAGGCTG TATTAGGAAC 
CTGTACATCA ATAATGAACT ACAGGACTTC AGAAAACTAA ATCTCCAAGC TGGAGTTCTT 
CCTGGCTGTG AACCTTGCCA TAAAAAAGTC TGTGTCCATG GCACATGTCT GGCTACTAGC 
CAAGCTGGCT TCACTTGCGA ATGCGAAGGA GGATGGACAG GACAACTGTG TGATATCCAG 
TCCAACGATC CCTGTCTGGG AAACAAGTGT GTCCATGGCA CGTGCCTCCC CATTAATTCA 
TTCTCCTACA GCTGTAAATG CCTTCAGGGC TTTGTGGGGG TTCTCTGTGA TGAACCTGAA 
GATCAGCCCA ATCCCTGCCA ATCCATTAAA TGTAAACACG GAAAATGCAG GCTTTCGGGC 
TTAGGGAAAG CCTATTGTGA ATGCAACAGT GGTTATACAG GGGACAGCTG TGATAAAGAA 
ATCTCTTGCC GAGGGGAACG GGTCAGAGAT TACTACCAAA GGCAGCAAGG TTATGCTGCT 
TGCCAGACGA CCGAGAAGGT ATCAAGACTA GAATGCAAAG GAGGATGCTC AAATGGTCAG 
TGCTGTGGGC CACTGAGGAG CAAAAGGCGG AAATATGCTT TTGAATGCAC AGATGGTTCA 
TCCTTTGTGG ACGAGGTGGA GAAAGTCATA AAGTGCGGTT GTACAAAGTG CCCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811160.1
CDS	734..5353
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKEISCRGERVRDYYQRQQGYAACQTTEKVSRLECKGGCSNGQCCGPLRSKRRKYAFECTDGSSFVDEVEKVIKCGCTKCPS

Target ORF information:

RefSeq Version	XM_012955706.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955706.3

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GCTGCTAAAT TGAATGAGGT GGAAAAAGGT 
GAACTTTGTG AAGAAAAACT GGATTTCTGC TCCCAAGATG TCAATCCATG TCAGCATGAC 
TCAAAATGCA TCCTGACACT GAAAGGCTAC AAATGTGACT GCACCCCCGG ATATATTGGG 
GAACACTGTG ATATCGACTA CGATGATTGT CAGGAGAACA AGTGCAGAAA TGGAGCCCGT 
TGCGTTGATG CAGTGAATGG TTATACTTGT GTCTGCCCAG AAGGATACAG CGGTTTGTTC 
TGTGAATCGG CGCCACCAAT GGTTTTACCT CGCACAAGTC CTTGTGACAA CTTTGAATGT 
CAAAATGGAG CCCAGTGTAT CGTGAAAGGA AATGAGCCTC AGTGCCAGTG CTTGCCCGGA 
TATCACGGAA ACAAATGTGA AAAACTAGTC AGTGTAAATT TTGTGAACAA AGACTCGTTT 
ATCCAAATTC CTTCGGCAAA GATCCGCACT CAGGCCAACA TCACTCTGCA GGTCGCCACG 
GATGAAGACA GTGGGATCTT ACTCTATAAA GGTGACAAGG ATCAGGTAGC GGTGGAGTTG 
TATCGTGGGA GAGTGAGAGT TAGTTACGAC ACTGGAATTA ACCCTGCATC TGCCATTTAC 
AGCGTTGAGA CTATAAATGA TGGAAATTTC CATGTTGTGG AATTGGTAAA CCTGGATCAA 
ACACTGTCAC TGGTCGTTGA TGGAGGAACC CCTAAATCCA TTACGAATTT ACCAAAACAG 
TCATTAATAA GTATTGACTC CACCCTTTAC ATAGGAGGTA TGCCCATGAA AAATAACATT 
GCAGGTCTTC GACAGTCTTC AAGTCAAAAT GGCACCAACT TCCTAGGCTG TATTAGGAAC 
CTGTACATCA ATAATGAACT ACAGGACTTC AGAAAACTAA ATCTCCAAGC TGGAGTTCTT 
CCTGGCTGTG AACCTTGCCA TAAAAAAGTC TGTGTCCATG GCACATGTCT GGCTACTAGC 
CAAGCTGGCT TCACTTGCGA ATGCGAAGGA GGATGGACAG GACAACTGTG TGATATCCAG 
TCCAACGATC CCTGTCTGGG AAACAAGTGT GTCCATGGCA CGTGCCTCCC CATTAATTCA 
TTCTCCTACA GCTGTAAATG CCTTCAGGGC TTTGTGGGGG TTCTCTGTGA TGAACCTGAA 
GATCAGCCCA ATCCCTGCCA ATCCATTAAA TGTAAACACG GAAAATGCAG GCTTTCGGGC 
TTAGGGAAAG CCTATTGTGA ATGCAACAGT GGTTATACAG GGGACAGCTG TGATAAAGAA 
ATCTCTTGCC GAGGGGAACG GGTCAGAGAT TACTACCAAA GGCAGCAAGG TTATGCTGCT 
TGCCAGACGA CCGAGAAGGT ATCAAGACTA GAATGCAAAG GAGGATGCTC AAATGGTCAG 
TGCTGTGGGC CACTGAGGAG CAAAAGGCGG AAATATGCTT TTGAATGCAC AGATGGTTCA 
TCCTTTGTGG ACGAGGTGGA GAAAGTCATA AAGTGCGGTT GTACAAAGTG CCCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_031895349.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751209.1 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X1, mRNA.

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CloneID	OXa49659
Clone ID Related Accession (Same CDS sequence)	XM_031895349.1
Accession Version	XM_031895349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGATGAGGAA GAAGGTCAGC AGTCATTTAT GGTCCATTCT 
TGTAGCGTCT TGCATTGCCC TGCCGCTTGT ACATGCAGCA ACAATATTGT AGATTGCCGT 
GGAAAGGGGC TGACTGAGAT ACCCACCAAC CTCCCGGAGA CAATAACAGA AATGAGACTG 
GAGCAAAATT CGATCAAGGT CATTCCTCCA GGAGCATTCT CACCATATAA AAAGTTGAGA 
AGAATTGATT TAAGCAACAA CCAGATTTCT GAAATAGCCG CAGATGCTTT CCAAGGCCTG 
CGTTCACTCA ACTCCCTTGT TCTCTATGGA AATAAAATCA CAGAGCTCCC TAAAGGCTTG 
TTTGAAGGAC TGGTCTTATT ACAGTTGCTG TTACTCAATG CCAACAAGAT TAATTGTTTA 
CGAGTAGACT CCTTCCAAGA TCTGCACAAC CTGAACCTTC TATCTTTGTA CGACAACAAA 
CTTCAAACCA TCGCCAAAGG CACATTTGCC CCGCTCCGCG CGATCCAGAC GCTGCATTTG 
GCTCAGAACC CGTTTATCTG CGACTGCCAT CTGAAGTGGC TGGCGGATTA TCTCCATACA 
AACCCCATTG AGACCAGTGG TGCCCGCTGC ACCAGCCCCC GCCGACTGGC AAACAAAAGG 
ATTGGGCAGA TCAAAAGCAA GAAATTCCGC TGCTCAGGCA CAGAAGACTA CCGATCCAAG 
CTGAGCGGAG ATTGTTTTGC AGACTTGGCC TGCCCAGAAA AATGTCGCTG TGAAGGAACC 
ACTGTGGATT GTTCCAACCA AAAACTTACC AAGATTCCTG ACCATATTCC CCAGTACACA 
GCTGAGTTGC GCCTCAACAA CAACGAATTC ACAGTTTTGG AGGCAACTGG AATCTTCAAG 
AAACTTCCCC AGCTTAGAAA AATAAACCTG AGCAACAACA AGATAACAGA TATTGAAGAA 
GGGGCTTTCG AAGGTGCCAA TGGCGTGAAT GAGCTACTGC TAACAAGTAA TCGCATGGAA 
AATGTGCGCC ATAAGATGTT CAAGGGCCTG GAAGGATTGA AAACCCTGAT GCTCAGAAGC 
AATCGCATTA GCTGTGTGAA TAATGACAGT TTCACCGGGC TGAGCTCGGT TCGCCTGCTC 
TCCCTCTATG ACAATCAAAT TACTACAGTG GCTCCCGGTG CATTCGACAC GCTGCATTCT 
CTCTCTACAT TGAACCTTTT AGCGAATCCA TTTAATTGCA ACTGCCACCT GGCTTGGTTG 
GGCGACTGGC TTAGGAAGAA GCGCATTGTC ACAGGAAACC CTCGATGCCA AAAGCCCTAT 
TTCCTTAAGG AGATTCCAAT CCAGGATGTG GCTATACAAG ATTTTACTTG TGATGATGGG 
AATGAGGACA ATAGCTGTTC GCCGCTGTCT CGCTGCCCTG CCGAGTGCAC TTGTTTAGAT 
ACTGTTGTTC GATGCAGTAA CAAAGGACTG AAGTCTTTAC CCAAAGGCAT CCCTAAGGAG 
GTCACAGAAC TATATTTGGA TGGAAACATG TTTCCACTTG TTCCAAAGGA ACTTTCTAAC 
TATAAGCACT TGACCCTTAT CGACCTGAGC AACAACCGAA TCAGCACTCT TTCTAACCAC 
AGCTTCAGCA ACATGACCCA GCTCCTCACC CTGATTCTTA GTTACAATCG CCTCCGGTGC 
ATTCCACTCC GGGCATTTGA TGGGTTGAAG TCTCTGAGAC TTTTATCCTT GCACGGCAAT 
GACGTCTCTG CTATACCTGA AGGGGCATTC AATGATCTTT CCTCACTGTC ACATCTAGCT 
ATTGGGGCGA ACCCTCTGTA TTGTGATTGT AACCTGCAGT GGCTTTCCGA CTGGGTGAAG 
TCAGAATATA AAGAACCTGG GATCGCAAGG TGTTCTGGGC CTGGGGAAAT GGCTGATAAG 
CTCCTCCTCA CCACCCCCTC CAAAAAGTTC ACCTGCAATG GGCCTGTAGA TGTCAGCATT 
CTTGCTAAAT GTAACCCCTG CTTATCCAAT CCTTGCAAGA ACGACGGCAC CTGTAACAAC 
GATCCTGTCG AATTCTACCG ATGCACATGT CCCTATGGCT TTAAGGGACA AGACTGCGAT 
GTACCAATCC ATGCCTGCAT CAGTAACCCG TGTCGGAATA GTGGTACCTG CCATTTAAAG 
GAAGGGGACA AAGACGGCTT CTGGTGCGCT TGTGCCGAAG GATTCGAAGG TGAAAACTGC 
GAGGTCAACA TTGACGACTG TGAAGATAAC GACTGCGAGA ATAATTCTAC TTGTGTCGAC 
GGCATTAACA ATTACACGTG CCGCTGCCCA CCGGAATACA CAGCTGCTAA ATTGAATGAG 
GTGGAAAAAG GTGAACTTTG TGAAGAAAAA CTGGATTTCT GCTCCCAAGA TGTCAATCCA 
TGTCAGCATG ACTCAAAATG CATCCTGACA CTGAAAGGCT ACAAATGTGA CTGCACCCCC 
GGATATATTG GGGAACACTG TGATATCGAC TACGATGATT GTCAGGAGAA CAAGTGCAGA 
AATGGAGCCC GTTGCGTTGA TGCAGTGAAT GGTTATACTT GTGTCTGCCC AGAAGGATAC 
AGCGGTTTGT TCTGTGAATC GGCGCCACCA ATGGTTTTAC CTCGCACAAG TCCTTGTGAC 
AACTTTGAAT GTCAAAATGG AGCCCAGTGT ATCGTGAAAG GAAATGAGCC TCAGTGCCAG 
TGCTTGCCCG GATATCACGG AAACAAATGT GAAAAACTAG TCAGTGTAAA TTTTGTGAAC 
AAAGACTCGT TTATCCAAAT TCCTTCGGCA AAGATCCGCA CTCAGGCCAA CATCACTCTG 
CAGGTCGCCA CGGATGAAGA CAGTGGGATC TTACTCTATA AAGGTGACAA GGATCAGGTA 
GCGGTGGAGT TGTATCGTGG GAGAGTGAGA GTTAGTTACG ACACTGGAAT TAACCCTGCA 
TCTGCCATTT ACAGCGTTGA GACTATAAAT GATGGAAATT TCCATGTTGT GGAATTGGTA 
AACCTGGATC AAACACTGTC ACTGGTCGTT GATGGAGGAA CCCCTAAATC CATTACGAAT 
TTACCAAAAC AGTCATTAAT AAGTATTGAC TCCACCCTTT ACATAGGAGG TATGCCCATG 
AAAAATAACA TTGCAGGTCT TCGACAGTCT TCAAGTCAAA ATGGCACCAA CTTCCTAGGC 
TGTATTAGGA ACCTGTACAT CAATAATGAA CTACAGGACT TCAGAAAACT AAATCTCCAA 
GCTGGAGTTC TTCCTGGCTG TGAACCTTGC CATAAAAAAG TCTGTGTCCA TGGCACATGT 
CTGGCTACTA GCCAAGCTGG CTTCACTTGC GAATGCGAAG GAGGATGGAC AGGACAACTG 
TGTGATATCC AGTCCAACGA TCCCTGTCTG GGAAACAAGT GTGTCCATGG CACGTGCCTC 
CCCATTAATT CATTCTCCTA CAGCTGTAAA TGCCTTCAGG GCTTTGTGGG GGTTCTCTGT 
GATGAACCTG AAGATCAGCC CAATCCCTGC CAATCCATTA AATGTAAACA CGGAAAATGC 
AGGCTTTCGG GCTTAGGGAA AGCCTATTGT GAATGCAACA GTGGTTATAC AGGGGACAGC 
TGTGATAAAG AAATCTCTTG CCGAGGGGAA CGGGTCAGAG ATTACTACCA AAGGCAGCAA 
GGTTATGCTG CTTGCCAGAC GACCGAGAAG GTATCAAGAC TAGAATGCAA AGGAGGATGC 
TCAAATGGTC AGTGCTGTGG GCCACTGAGG AGCAAAAGGC GGAAATATGC TTTTGAATGC 
ACAGATGGTT CATCCTTTGT GGACGAGGTG GAGAAAGTCA TAAAGTGCGG TTGTACAAAG 
TGCCCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751209.1
CDS	735..5366
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTDEEEGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKEISCRGERVRDYYQRQQGYAACQTTEKVSRLECKGGCSNGQCCGPLRSKRRKYAFECTDGSSFVDEVEKVIKCGCTKCPS

Target ORF information:

RefSeq Version	XM_031895349.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895349.1

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGATGAGGAA GAAGGTCAGC AGTCATTTAT GGTCCATTCT 
TGTAGCGTCT TGCATTGCCC TGCCGCTTGT ACATGCAGCA ACAATATTGT AGATTGCCGT 
GGAAAGGGGC TGACTGAGAT ACCCACCAAC CTCCCGGAGA CAATAACAGA AATGAGACTG 
GAGCAAAATT CGATCAAGGT CATTCCTCCA GGAGCATTCT CACCATATAA AAAGTTGAGA 
AGAATTGATT TAAGCAACAA CCAGATTTCT GAAATAGCCG CAGATGCTTT CCAAGGCCTG 
CGTTCACTCA ACTCCCTTGT TCTCTATGGA AATAAAATCA CAGAGCTCCC TAAAGGCTTG 
TTTGAAGGAC TGGTCTTATT ACAGTTGCTG TTACTCAATG CCAACAAGAT TAATTGTTTA 
CGAGTAGACT CCTTCCAAGA TCTGCACAAC CTGAACCTTC TATCTTTGTA CGACAACAAA 
CTTCAAACCA TCGCCAAAGG CACATTTGCC CCGCTCCGCG CGATCCAGAC GCTGCATTTG 
GCTCAGAACC CGTTTATCTG CGACTGCCAT CTGAAGTGGC TGGCGGATTA TCTCCATACA 
AACCCCATTG AGACCAGTGG TGCCCGCTGC ACCAGCCCCC GCCGACTGGC AAACAAAAGG 
ATTGGGCAGA TCAAAAGCAA GAAATTCCGC TGCTCAGGCA CAGAAGACTA CCGATCCAAG 
CTGAGCGGAG ATTGTTTTGC AGACTTGGCC TGCCCAGAAA AATGTCGCTG TGAAGGAACC 
ACTGTGGATT GTTCCAACCA AAAACTTACC AAGATTCCTG ACCATATTCC CCAGTACACA 
GCTGAGTTGC GCCTCAACAA CAACGAATTC ACAGTTTTGG AGGCAACTGG AATCTTCAAG 
AAACTTCCCC AGCTTAGAAA AATAAACCTG AGCAACAACA AGATAACAGA TATTGAAGAA 
GGGGCTTTCG AAGGTGCCAA TGGCGTGAAT GAGCTACTGC TAACAAGTAA TCGCATGGAA 
AATGTGCGCC ATAAGATGTT CAAGGGCCTG GAAGGATTGA AAACCCTGAT GCTCAGAAGC 
AATCGCATTA GCTGTGTGAA TAATGACAGT TTCACCGGGC TGAGCTCGGT TCGCCTGCTC 
TCCCTCTATG ACAATCAAAT TACTACAGTG GCTCCCGGTG CATTCGACAC GCTGCATTCT 
CTCTCTACAT TGAACCTTTT AGCGAATCCA TTTAATTGCA ACTGCCACCT GGCTTGGTTG 
GGCGACTGGC TTAGGAAGAA GCGCATTGTC ACAGGAAACC CTCGATGCCA AAAGCCCTAT 
TTCCTTAAGG AGATTCCAAT CCAGGATGTG GCTATACAAG ATTTTACTTG TGATGATGGG 
AATGAGGACA ATAGCTGTTC GCCGCTGTCT CGCTGCCCTG CCGAGTGCAC TTGTTTAGAT 
ACTGTTGTTC GATGCAGTAA CAAAGGACTG AAGTCTTTAC CCAAAGGCAT CCCTAAGGAG 
GTCACAGAAC TATATTTGGA TGGAAACATG TTTCCACTTG TTCCAAAGGA ACTTTCTAAC 
TATAAGCACT TGACCCTTAT CGACCTGAGC AACAACCGAA TCAGCACTCT TTCTAACCAC 
AGCTTCAGCA ACATGACCCA GCTCCTCACC CTGATTCTTA GTTACAATCG CCTCCGGTGC 
ATTCCACTCC GGGCATTTGA TGGGTTGAAG TCTCTGAGAC TTTTATCCTT GCACGGCAAT 
GACGTCTCTG CTATACCTGA AGGGGCATTC AATGATCTTT CCTCACTGTC ACATCTAGCT 
ATTGGGGCGA ACCCTCTGTA TTGTGATTGT AACCTGCAGT GGCTTTCCGA CTGGGTGAAG 
TCAGAATATA AAGAACCTGG GATCGCAAGG TGTTCTGGGC CTGGGGAAAT GGCTGATAAG 
CTCCTCCTCA CCACCCCCTC CAAAAAGTTC ACCTGCAATG GGCCTGTAGA TGTCAGCATT 
CTTGCTAAAT GTAACCCCTG CTTATCCAAT CCTTGCAAGA ACGACGGCAC CTGTAACAAC 
GATCCTGTCG AATTCTACCG ATGCACATGT CCCTATGGCT TTAAGGGACA AGACTGCGAT 
GTACCAATCC ATGCCTGCAT CAGTAACCCG TGTCGGAATA GTGGTACCTG CCATTTAAAG 
GAAGGGGACA AAGACGGCTT CTGGTGCGCT TGTGCCGAAG GATTCGAAGG TGAAAACTGC 
GAGGTCAACA TTGACGACTG TGAAGATAAC GACTGCGAGA ATAATTCTAC TTGTGTCGAC 
GGCATTAACA ATTACACGTG CCGCTGCCCA CCGGAATACA CAGCTGCTAA ATTGAATGAG 
GTGGAAAAAG GTGAACTTTG TGAAGAAAAA CTGGATTTCT GCTCCCAAGA TGTCAATCCA 
TGTCAGCATG ACTCAAAATG CATCCTGACA CTGAAAGGCT ACAAATGTGA CTGCACCCCC 
GGATATATTG GGGAACACTG TGATATCGAC TACGATGATT GTCAGGAGAA CAAGTGCAGA 
AATGGAGCCC GTTGCGTTGA TGCAGTGAAT GGTTATACTT GTGTCTGCCC AGAAGGATAC 
AGCGGTTTGT TCTGTGAATC GGCGCCACCA ATGGTTTTAC CTCGCACAAG TCCTTGTGAC 
AACTTTGAAT GTCAAAATGG AGCCCAGTGT ATCGTGAAAG GAAATGAGCC TCAGTGCCAG 
TGCTTGCCCG GATATCACGG AAACAAATGT GAAAAACTAG TCAGTGTAAA TTTTGTGAAC 
AAAGACTCGT TTATCCAAAT TCCTTCGGCA AAGATCCGCA CTCAGGCCAA CATCACTCTG 
CAGGTCGCCA CGGATGAAGA CAGTGGGATC TTACTCTATA AAGGTGACAA GGATCAGGTA 
GCGGTGGAGT TGTATCGTGG GAGAGTGAGA GTTAGTTACG ACACTGGAAT TAACCCTGCA 
TCTGCCATTT ACAGCGTTGA GACTATAAAT GATGGAAATT TCCATGTTGT GGAATTGGTA 
AACCTGGATC AAACACTGTC ACTGGTCGTT GATGGAGGAA CCCCTAAATC CATTACGAAT 
TTACCAAAAC AGTCATTAAT AAGTATTGAC TCCACCCTTT ACATAGGAGG TATGCCCATG 
AAAAATAACA TTGCAGGTCT TCGACAGTCT TCAAGTCAAA ATGGCACCAA CTTCCTAGGC 
TGTATTAGGA ACCTGTACAT CAATAATGAA CTACAGGACT TCAGAAAACT AAATCTCCAA 
GCTGGAGTTC TTCCTGGCTG TGAACCTTGC CATAAAAAAG TCTGTGTCCA TGGCACATGT 
CTGGCTACTA GCCAAGCTGG CTTCACTTGC GAATGCGAAG GAGGATGGAC AGGACAACTG 
TGTGATATCC AGTCCAACGA TCCCTGTCTG GGAAACAAGT GTGTCCATGG CACGTGCCTC 
CCCATTAATT CATTCTCCTA CAGCTGTAAA TGCCTTCAGG GCTTTGTGGG GGTTCTCTGT 
GATGAACCTG AAGATCAGCC CAATCCCTGC CAATCCATTA AATGTAAACA CGGAAAATGC 
AGGCTTTCGG GCTTAGGGAA AGCCTATTGT GAATGCAACA GTGGTTATAC AGGGGACAGC 
TGTGATAAAG AAATCTCTTG CCGAGGGGAA CGGGTCAGAG ATTACTACCA AAGGCAGCAA 
GGTTATGCTG CTTGCCAGAC GACCGAGAAG GTATCAAGAC TAGAATGCAA AGGAGGATGC 
TCAAATGGTC AGTGCTGTGG GCCACTGAGG AGCAAAAGGC GGAAATATGC TTTTGAATGC 
ACAGATGGTT CATCCTTTGT GGACGAGGTG GAGAAAGTCA TAAAGTGCGG TTGTACAAAG 
TGCCCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_031895350.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751210.1 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X3, mRNA.

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CloneID	OXa49660
Clone ID Related Accession (Same CDS sequence)	XM_031895350.1
Accession Version	XM_031895350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGATGAGGAA GAAGGTCAGC AGTCATTTAT GGTCCATTCT 
TGTAGCGTCT TGCATTGCCC TGCCGCTTGT ACATGCAGCA ACAATATTGT AGATTGCCGT 
GGAAAGGGGC TGACTGAGAT ACCCACCAAC CTCCCGGAGA CAATAACAGA AATGAGACTG 
GAGCAAAATT CGATCAAGGT CATTCCTCCA GGAGCATTCT CACCATATAA AAAGTTGAGA 
AGAATTGATT TAAGCAACAA CCAGATTTCT GAAATAGCCG CAGATGCTTT CCAAGGCCTG 
CGTTCACTCA ACTCCCTTGT TCTCTATGGA AATAAAATCA CAGAGCTCCC TAAAGGCTTG 
TTTGAAGGAC TGGTCTTATT ACAGTTGCTG TTACTCAATG CCAACAAGAT TAATTGTTTA 
CGAGTAGACT CCTTCCAAGA TCTGCACAAC CTGAACCTTC TATCTTTGTA CGACAACAAA 
CTTCAAACCA TCGCCAAAGG CACATTTGCC CCGCTCCGCG CGATCCAGAC GCTGCATTTG 
GCTCAGAACC CGTTTATCTG CGACTGCCAT CTGAAGTGGC TGGCGGATTA TCTCCATACA 
AACCCCATTG AGACCAGTGG TGCCCGCTGC ACCAGCCCCC GCCGACTGGC AAACAAAAGG 
ATTGGGCAGA TCAAAAGCAA GAAATTCCGC TGCTCAGGCA CAGAAGACTA CCGATCCAAG 
CTGAGCGGAG ATTGTTTTGC AGACTTGGCC TGCCCAGAAA AATGTCGCTG TGAAGGAACC 
ACTGTGGATT GTTCCAACCA AAAACTTACC AAGATTCCTG ACCATATTCC CCAGTACACA 
GCTGAGTTGC GCCTCAACAA CAACGAATTC ACAGTTTTGG AGGCAACTGG AATCTTCAAG 
AAACTTCCCC AGCTTAGAAA AATAAACCTG AGCAACAACA AGATAACAGA TATTGAAGAA 
GGGGCTTTCG AAGGTGCCAA TGGCGTGAAT GAGCTACTGC TAACAAGTAA TCGCATGGAA 
AATGTGCGCC ATAAGATGTT CAAGGGCCTG GAAGGATTGA AAACCCTGAT GCTCAGAAGC 
AATCGCATTA GCTGTGTGAA TAATGACAGT TTCACCGGGC TGAGCTCGGT TCGCCTGCTC 
TCCCTCTATG ACAATCAAAT TACTACAGTG GCTCCCGGTG CATTCGACAC GCTGCATTCT 
CTCTCTACAT TGAACCTTTT AGCGAATCCA TTTAATTGCA ACTGCCACCT GGCTTGGTTG 
GGCGACTGGC TTAGGAAGAA GCGCATTGTC ACAGGAAACC CTCGATGCCA AAAGCCCTAT 
TTCCTTAAGG AGATTCCAAT CCAGGATGTG GCTATACAAG ATTTTACTTG TGATGATGGG 
AATGAGGACA ATAGCTGTTC GCCGCTGTCT CGCTGCCCTG CCGAGTGCAC TTGTTTAGAT 
ACTGTTGTTC GATGCAGTAA CAAAGGACTG AAGTCTTTAC CCAAAGGCAT CCCTAAGGAG 
GTCACAGAAC TATATTTGGA TGGAAACATG TTTCCACTTG TTCCAAAGGA ACTTTCTAAC 
TATAAGCACT TGACCCTTAT CGACCTGAGC AACAACCGAA TCAGCACTCT TTCTAACCAC 
AGCTTCAGCA ACATGACCCA GCTCCTCACC CTGATTCTTA GTTACAATCG CCTCCGGTGC 
ATTCCACTCC GGGCATTTGA TGGGTTGAAG TCTCTGAGAC TTTTATCCTT GCACGGCAAT 
GACGTCTCTG CTATACCTGA AGGGGCATTC AATGATCTTT CCTCACTGTC ACATCTAGCT 
ATTGGGGCGA ACCCTCTGTA TTGTGATTGT AACCTGCAGT GGCTTTCCGA CTGGGTGAAG 
TCAGAATATA AAGAACCTGG GATCGCAAGG TGTTCTGGGC CTGGGGAAAT GGCTGATAAG 
CTCCTCCTCA CCACCCCCTC CAAAAAGTTC ACCTGCAATG GGCCTGTAGA TGTCAGCATT 
CTTGCTAAAT GTAACCCCTG CTTATCCAAT CCTTGCAAGA ACGACGGCAC CTGTAACAAC 
GATCCTGTCG AATTCTACCG ATGCACATGT CCCTATGGCT TTAAGGGACA AGACTGCGAT 
GTACCAATCC ATGCCTGCAT CAGTAACCCG TGTCGGAATA GTGGTACCTG CCATTTAAAG 
GAAGGGGACA AAGACGGCTT CTGGTGCGCT TGTGCCGAAG GATTCGAAGG TGAAAACTGC 
GAGGTCAACA TTGACGACTG TGAAGATAAC GACTGCGAGA ATAATTCTAC TTGTGTCGAC 
GGCATTAACA ATTACACGTG CCGCTGCCCA CCGGAATACA CAGGTGAACT TTGTGAAGAA 
AAACTGGATT TCTGCTCCCA AGATGTCAAT CCATGTCAGC ATGACTCAAA ATGCATCCTG 
ACACTGAAAG GCTACAAATG TGACTGCACC CCCGGATATA TTGGGGAACA CTGTGATATC 
GACTACGATG ATTGTCAGGA GAACAAGTGC AGAAATGGAG CCCGTTGCGT TGATGCAGTG 
AATGGTTATA CTTGTGTCTG CCCAGAAGGA TACAGCGGTT TGTTCTGTGA ATCGGCGCCA 
CCAATGGTTT TACCTCGCAC AAGTCCTTGT GACAACTTTG AATGTCAAAA TGGAGCCCAG 
TGTATCGTGA AAGGAAATGA GCCTCAGTGC CAGTGCTTGC CCGGATATCA CGGAAACAAA 
TGTGAAAAAC TAGTCAGTGT AAATTTTGTG AACAAAGACT CGTTTATCCA AATTCCTTCG 
GCAAAGATCC GCACTCAGGC CAACATCACT CTGCAGGTCG CCACGGATGA AGACAGTGGG 
ATCTTACTCT ATAAAGGTGA CAAGGATCAG GTAGCGGTGG AGTTGTATCG TGGGAGAGTG 
AGAGTTAGTT ACGACACTGG AATTAACCCT GCATCTGCCA TTTACAGCGT TGAGACTATA 
AATGATGGAA ATTTCCATGT TGTGGAATTG GTAAACCTGG ATCAAACACT GTCACTGGTC 
GTTGATGGAG GAACCCCTAA ATCCATTACG AATTTACCAA AACAGTCATT AATAAGTATT 
GACTCCACCC TTTACATAGG AGGTATGCCC ATGAAAAATA ACATTGCAGG TCTTCGACAG 
TCTTCAAGTC AAAATGGCAC CAACTTCCTA GGCTGTATTA GGAACCTGTA CATCAATAAT 
GAACTACAGG ACTTCAGAAA ACTAAATCTC CAAGCTGGAG TTCTTCCTGG CTGTGAACCT 
TGCCATAAAA AAGTCTGTGT CCATGGCACA TGTCTGGCTA CTAGCCAAGC TGGCTTCACT 
TGCGAATGCG AAGGAGGATG GACAGGACAA CTGTGTGATA TCCAGTCCAA CGATCCCTGT 
CTGGGAAACA AGTGTGTCCA TGGCACGTGC CTCCCCATTA ATTCATTCTC CTACAGCTGT 
AAATGCCTTC AGGGCTTTGT GGGGGTTCTC TGTGATGAAC CTGAAGATCA GCCCAATCCC 
TGCCAATCCA TTAAATGTAA ACACGGAAAA TGCAGGCTTT CGGGCTTAGG GAAAGCCTAT 
TGTGAATGCA ACAGTGGTTA TACAGGGGAC AGCTGTGATA AAGAAATCTC TTGCCGAGGG 
GAACGGGTCA GAGATTACTA CCAAAGGCAG CAAGGTTATG CTGCTTGCCA GACGACCGAG 
AAGGTATCAA GACTAGAATG CAAAGGAGGA TGCTCAAATG GTCAGTGCTG TGGGCCACTG 
AGGAGCAAAA GGCGGAAATA TGCTTTTGAA TGCACAGATG GTTCATCCTT TGTGGACGAG 
GTGGAGAAAG TCATAAAGTG CGGTTGTACA AAGTGCCCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751210.1
CDS	733..5337
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTDEEEGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKEISCRGERVRDYYQRQQGYAACQTTEKVSRLECKGGCSNGQCCGPLRSKRRKYAFECTDGSSFVDEVEKVIKCGCTKCPS

Target ORF information:

RefSeq Version	XM_031895350.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895350.1

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGATGAGGAA GAAGGTCAGC AGTCATTTAT GGTCCATTCT 
TGTAGCGTCT TGCATTGCCC TGCCGCTTGT ACATGCAGCA ACAATATTGT AGATTGCCGT 
GGAAAGGGGC TGACTGAGAT ACCCACCAAC CTCCCGGAGA CAATAACAGA AATGAGACTG 
GAGCAAAATT CGATCAAGGT CATTCCTCCA GGAGCATTCT CACCATATAA AAAGTTGAGA 
AGAATTGATT TAAGCAACAA CCAGATTTCT GAAATAGCCG CAGATGCTTT CCAAGGCCTG 
CGTTCACTCA ACTCCCTTGT TCTCTATGGA AATAAAATCA CAGAGCTCCC TAAAGGCTTG 
TTTGAAGGAC TGGTCTTATT ACAGTTGCTG TTACTCAATG CCAACAAGAT TAATTGTTTA 
CGAGTAGACT CCTTCCAAGA TCTGCACAAC CTGAACCTTC TATCTTTGTA CGACAACAAA 
CTTCAAACCA TCGCCAAAGG CACATTTGCC CCGCTCCGCG CGATCCAGAC GCTGCATTTG 
GCTCAGAACC CGTTTATCTG CGACTGCCAT CTGAAGTGGC TGGCGGATTA TCTCCATACA 
AACCCCATTG AGACCAGTGG TGCCCGCTGC ACCAGCCCCC GCCGACTGGC AAACAAAAGG 
ATTGGGCAGA TCAAAAGCAA GAAATTCCGC TGCTCAGGCA CAGAAGACTA CCGATCCAAG 
CTGAGCGGAG ATTGTTTTGC AGACTTGGCC TGCCCAGAAA AATGTCGCTG TGAAGGAACC 
ACTGTGGATT GTTCCAACCA AAAACTTACC AAGATTCCTG ACCATATTCC CCAGTACACA 
GCTGAGTTGC GCCTCAACAA CAACGAATTC ACAGTTTTGG AGGCAACTGG AATCTTCAAG 
AAACTTCCCC AGCTTAGAAA AATAAACCTG AGCAACAACA AGATAACAGA TATTGAAGAA 
GGGGCTTTCG AAGGTGCCAA TGGCGTGAAT GAGCTACTGC TAACAAGTAA TCGCATGGAA 
AATGTGCGCC ATAAGATGTT CAAGGGCCTG GAAGGATTGA AAACCCTGAT GCTCAGAAGC 
AATCGCATTA GCTGTGTGAA TAATGACAGT TTCACCGGGC TGAGCTCGGT TCGCCTGCTC 
TCCCTCTATG ACAATCAAAT TACTACAGTG GCTCCCGGTG CATTCGACAC GCTGCATTCT 
CTCTCTACAT TGAACCTTTT AGCGAATCCA TTTAATTGCA ACTGCCACCT GGCTTGGTTG 
GGCGACTGGC TTAGGAAGAA GCGCATTGTC ACAGGAAACC CTCGATGCCA AAAGCCCTAT 
TTCCTTAAGG AGATTCCAAT CCAGGATGTG GCTATACAAG ATTTTACTTG TGATGATGGG 
AATGAGGACA ATAGCTGTTC GCCGCTGTCT CGCTGCCCTG CCGAGTGCAC TTGTTTAGAT 
ACTGTTGTTC GATGCAGTAA CAAAGGACTG AAGTCTTTAC CCAAAGGCAT CCCTAAGGAG 
GTCACAGAAC TATATTTGGA TGGAAACATG TTTCCACTTG TTCCAAAGGA ACTTTCTAAC 
TATAAGCACT TGACCCTTAT CGACCTGAGC AACAACCGAA TCAGCACTCT TTCTAACCAC 
AGCTTCAGCA ACATGACCCA GCTCCTCACC CTGATTCTTA GTTACAATCG CCTCCGGTGC 
ATTCCACTCC GGGCATTTGA TGGGTTGAAG TCTCTGAGAC TTTTATCCTT GCACGGCAAT 
GACGTCTCTG CTATACCTGA AGGGGCATTC AATGATCTTT CCTCACTGTC ACATCTAGCT 
ATTGGGGCGA ACCCTCTGTA TTGTGATTGT AACCTGCAGT GGCTTTCCGA CTGGGTGAAG 
TCAGAATATA AAGAACCTGG GATCGCAAGG TGTTCTGGGC CTGGGGAAAT GGCTGATAAG 
CTCCTCCTCA CCACCCCCTC CAAAAAGTTC ACCTGCAATG GGCCTGTAGA TGTCAGCATT 
CTTGCTAAAT GTAACCCCTG CTTATCCAAT CCTTGCAAGA ACGACGGCAC CTGTAACAAC 
GATCCTGTCG AATTCTACCG ATGCACATGT CCCTATGGCT TTAAGGGACA AGACTGCGAT 
GTACCAATCC ATGCCTGCAT CAGTAACCCG TGTCGGAATA GTGGTACCTG CCATTTAAAG 
GAAGGGGACA AAGACGGCTT CTGGTGCGCT TGTGCCGAAG GATTCGAAGG TGAAAACTGC 
GAGGTCAACA TTGACGACTG TGAAGATAAC GACTGCGAGA ATAATTCTAC TTGTGTCGAC 
GGCATTAACA ATTACACGTG CCGCTGCCCA CCGGAATACA CAGGTGAACT TTGTGAAGAA 
AAACTGGATT TCTGCTCCCA AGATGTCAAT CCATGTCAGC ATGACTCAAA ATGCATCCTG 
ACACTGAAAG GCTACAAATG TGACTGCACC CCCGGATATA TTGGGGAACA CTGTGATATC 
GACTACGATG ATTGTCAGGA GAACAAGTGC AGAAATGGAG CCCGTTGCGT TGATGCAGTG 
AATGGTTATA CTTGTGTCTG CCCAGAAGGA TACAGCGGTT TGTTCTGTGA ATCGGCGCCA 
CCAATGGTTT TACCTCGCAC AAGTCCTTGT GACAACTTTG AATGTCAAAA TGGAGCCCAG 
TGTATCGTGA AAGGAAATGA GCCTCAGTGC CAGTGCTTGC CCGGATATCA CGGAAACAAA 
TGTGAAAAAC TAGTCAGTGT AAATTTTGTG AACAAAGACT CGTTTATCCA AATTCCTTCG 
GCAAAGATCC GCACTCAGGC CAACATCACT CTGCAGGTCG CCACGGATGA AGACAGTGGG 
ATCTTACTCT ATAAAGGTGA CAAGGATCAG GTAGCGGTGG AGTTGTATCG TGGGAGAGTG 
AGAGTTAGTT ACGACACTGG AATTAACCCT GCATCTGCCA TTTACAGCGT TGAGACTATA 
AATGATGGAA ATTTCCATGT TGTGGAATTG GTAAACCTGG ATCAAACACT GTCACTGGTC 
GTTGATGGAG GAACCCCTAA ATCCATTACG AATTTACCAA AACAGTCATT AATAAGTATT 
GACTCCACCC TTTACATAGG AGGTATGCCC ATGAAAAATA ACATTGCAGG TCTTCGACAG 
TCTTCAAGTC AAAATGGCAC CAACTTCCTA GGCTGTATTA GGAACCTGTA CATCAATAAT 
GAACTACAGG ACTTCAGAAA ACTAAATCTC CAAGCTGGAG TTCTTCCTGG CTGTGAACCT 
TGCCATAAAA AAGTCTGTGT CCATGGCACA TGTCTGGCTA CTAGCCAAGC TGGCTTCACT 
TGCGAATGCG AAGGAGGATG GACAGGACAA CTGTGTGATA TCCAGTCCAA CGATCCCTGT 
CTGGGAAACA AGTGTGTCCA TGGCACGTGC CTCCCCATTA ATTCATTCTC CTACAGCTGT 
AAATGCCTTC AGGGCTTTGT GGGGGTTCTC TGTGATGAAC CTGAAGATCA GCCCAATCCC 
TGCCAATCCA TTAAATGTAA ACACGGAAAA TGCAGGCTTT CGGGCTTAGG GAAAGCCTAT 
TGTGAATGCA ACAGTGGTTA TACAGGGGAC AGCTGTGATA AAGAAATCTC TTGCCGAGGG 
GAACGGGTCA GAGATTACTA CCAAAGGCAG CAAGGTTATG CTGCTTGCCA GACGACCGAG 
AAGGTATCAA GACTAGAATG CAAAGGAGGA TGCTCAAATG GTCAGTGCTG TGGGCCACTG 
AGGAGCAAAA GGCGGAAATA TGCTTTTGAA TGCACAGATG GTTCATCCTT TGTGGACGAG 
GTGGAGAAAG TCATAAAGTG CGGTTGTACA AAGTGCCCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_031895351.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031751211.1 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X5, mRNA.

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CloneID	OXa49661
Clone ID Related Accession (Same CDS sequence)	XM_031895351.1
Accession Version	XM_031895351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGATGAGGAA GAAGGTCAGC AGTCATTTAT GGTCCATTCT 
TGTAGCGTCT TGCATTGCCC TGCCGCTTGT ACATGCAGCA ACAATATTGT AGATTGCCGT 
GGAAAGGGGC TGACTGAGAT ACCCACCAAC CTCCCGGAGA CAATAACAGA AATGAGACTG 
GAGCAAAATT CGATCAAGGT CATTCCTCCA GGAGCATTCT CACCATATAA AAAGTTGAGA 
AGAATTGATT TAAGCAACAA CCAGATTTCT GAAATAGCCG CAGATGCTTT CCAAGGCCTG 
CGTTCACTCA ACTCCCTTGT TCTCTATGGA AATAAAATCA CAGAGCTCCC TAAAGGCTTG 
TTTGAAGGAC TGGTCTTATT ACAGTTGCTG TTACTCAATG CCAACAAGAT TAATTGTTTA 
CGAGTAGACT CCTTCCAAGA TCTGCACAAC CTGAACCTTC TATCTTTGTA CGACAACAAA 
CTTCAAACCA TCGCCAAAGG CACATTTGCC CCGCTCCGCG CGATCCAGAC GCTGCATTTG 
GCTCAGAACC CGTTTATCTG CGACTGCCAT CTGAAGTGGC TGGCGGATTA TCTCCATACA 
AACCCCATTG AGACCAGTGG TGCCCGCTGC ACCAGCCCCC GCCGACTGGC AAACAAAAGG 
ATTGGGCAGA TCAAAAGCAA GAAATTCCGC TGCTCAGGCA CAGAAGACTA CCGATCCAAG 
CTGAGCGGAG ATTGTTTTGC AGACTTGGCC TGCCCAGAAA AATGTCGCTG TGAAGGAACC 
ACTGTGGATT GTTCCAACCA AAAACTTACC AAGATTCCTG ACCATATTCC CCAGTACACA 
GCTGAGTTGC GCCTCAACAA CAACGAATTC ACAGTTTTGG AGGCAACTGG AATCTTCAAG 
AAACTTCCCC AGCTTAGAAA AATAAACCTG AGCAACAACA AGATAACAGA TATTGAAGAA 
GGGGCTTTCG AAGGTGCCAA TGGCGTGAAT GAGCTACTGC TAACAAGTAA TCGCATGGAA 
AATGTGCGCC ATAAGATGTT CAAGGGCCTG GAAGGATTGA AAACCCTGAT GCTCAGAAGC 
AATCGCATTA GCTGTGTGAA TAATGACAGT TTCACCGGGC TGAGCTCGGT TCGCCTGCTC 
TCCCTCTATG ACAATCAAAT TACTACAGTG GCTCCCGGTG CATTCGACAC GCTGCATTCT 
CTCTCTACAT TGAACCTTTT AGCGAATCCA TTTAATTGCA ACTGCCACCT GGCTTGGTTG 
GGCGACTGGC TTAGGAAGAA GCGCATTGTC ACAGGAAACC CTCGATGCCA AAAGCCCTAT 
TTCCTTAAGG AGATTCCAAT CCAGGATGTG GCTATACAAG ATTTTACTTG TGATGATGGG 
AATGAGGACA ATAGCTGTTC GCCGCTGTCT CGCTGCCCTG CCGAGTGCAC TTGTTTAGAT 
ACTGTTGTTC GATGCAGTAA CAAAGGACTG AAGTCTTTAC CCAAAGGCAT CCCTAAGGAG 
GTCACAGAAC TATATTTGGA TGGAAACATG TTTCCACTTG TTCCAAAGGA ACTTTCTAAC 
TATAAGCACT TGACCCTTAT CGACCTGAGC AACAACCGAA TCAGCACTCT TTCTAACCAC 
AGCTTCAGCA ACATGACCCA GCTCCTCACC CTGATTCTTA GTTACAATCG CCTCCGGTGC 
ATTCCACTCC GGGCATTTGA TGGGTTGAAG TCTCTGAGAC TTTTATCCTT GCACGGCAAT 
GACGTCTCTG CTATACCTGA AGGGGCATTC AATGATCTTT CCTCACTGTC ACATCTAGCT 
ATTGGGGCGA ACCCTCTGTA TTGTGATTGT AACCTGCAGT GGCTTTCCGA CTGGGTGAAG 
TCAGAATATA AAGAACCTGG GATCGCAAGG TGTTCTGGGC CTGGGGAAAT GGCTGATAAG 
CTCCTCCTCA CCACCCCCTC CAAAAAGTTC ACCTGCAATG GGCCTGTAGA TGTCAGCATT 
CTTGCTAAAT GTAACCCCTG CTTATCCAAT CCTTGCAAGA ACGACGGCAC CTGTAACAAC 
GATCCTGTCG AATTCTACCG ATGCACATGT CCCTATGGCT TTAAGGGACA AGACTGCGAT 
GTACCAATCC ATGCCTGCAT CAGTAACCCG TGTCGGAATA GTGGTACCTG CCATTTAAAG 
GAAGGGGACA AAGACGGCTT CTGGTGCGCT TGTGCCGAAG GATTCGAAGG TGAAAACTGC 
GAGGTCAACA TTGACGACTG TGAAGATAAC GACTGCGAGA ATAATTCTAC TTGTGTCGAC 
GGCATTAACA ATTACACGTG CCGCTGCCCA CCGGAATACA CAGCTGCTAA ATTGAATGAG 
GTGGAAAAAG GTGAACTTTG TGAAGAAAAA CTGGATTTCT GCTCCCAAGA TGTCAATCCA 
TGTCAGCATG ACTCAAAATG CATCCTGACA CTGAAAGGCT ACAAATGTGA CTGCACCCCC 
GGATATATTG GGGAACACTG TGATATCGAC TACGATGATT GTCAGGAGAA CAAGTGCAGA 
AATGGAGCCC GTTGCGTTGA TGCAGTGAAT GGTTATACTT GTGTCTGCCC AGAAGGATAC 
AGCGGTTTGT TCTGTGAATC GGCGCCACCA ATGGTTTTAC CTCGCACAAG TCCTTGTGAC 
AACTTTGAAT GTCAAAATGG AGCCCAGTGT ATCGTGAAAG GAAATGAGCC TCAGTGCCAG 
TGCTTGCCCG GATATCACGG AAACAAATGT GAAAAACTAG TCAGTGTAAA TTTTGTGAAC 
AAAGACTCGT TTATCCAAAT TCCTTCGGCA AAGATCCGCA CTCAGGCCAA CATCACTCTG 
CAGGTCGCCA CGGATGAAGA CAGTGGGATC TTACTCTATA AAGGTGACAA GGATCAGGTA 
GCGGTGGAGT TGTATCGTGG GAGAGTGAGA GTTAGTTACG ACACTGGAAT TAACCCTGCA 
TCTGCCATTT ACAGCGTTGA GACTATAAAT GATGGAAATT TCCATGTTGT GGAATTGGTA 
AACCTGGATC AAACACTGTC ACTGGTCGTT GATGGAGGAA CCCCTAAATC CATTACGAAT 
TTACCAAAAC AGTCATTAAT AAGTATTGAC TCCACCCTTT ACATAGGAGG TATGCCCATG 
AAAAATAACA TTGCAGGTCT TCGACAGTCT TCAAGTCAAA ATGGCACCAA CTTCCTAGGC 
TGTATTAGGA ACCTGTACAT CAATAATGAA CTACAGGACT TCAGAAAACT AAATCTCCAA 
GCTGGAGTTC TTCCTGGCTG TGAACCTTGC CATAAAAAAG TCTGTGTCCA TGGCACATGT 
CTGGCTACTA GCCAAGCTGG CTTCACTTGC GAATGCGAAG GAGGATGGAC AGGACAACTG 
TGTGATATCC AGTCCAACGA TCCCTGTCTG GGAAACAAGT GTGTCCATGG CACGTGCCTC 
CCCATTAATT CATTCTCCTA CAGCTGTAAA TGCCTTCAGG GCTTTGTGGG GGTTCTCTGT 
GATGAACCTG AAGATCAGCC CAATCCCTGC CAATCCATTA AATGTAAACA CGGAAAATGC 
AGGCTTTCGG GCTTAGGGAA AGCCTATTGT GAATGCAACA GTGGTTATAC AGGGGACAGC 
TGTGATAAAG CTTTCACTGA TTTATCCCAA CGAATTCCAC AGAAATCTCT TGCCGAGGGG 
AACGGGTCAG AGATTACTAC CAAAGGCAGC AAGGTTATGC TGCTTGCCAG ACGACCGAGA 
AGGTATCAAG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031751211.1
CDS	740..5254
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTDEEEGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKAFTDLSQRIPQKSLAEGNGSEITTKGSKVMLLARRPRRYQD

Target ORF information:

RefSeq Version	XM_031895351.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031895351.1

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGATGAGGAA GAAGGTCAGC AGTCATTTAT GGTCCATTCT 
TGTAGCGTCT TGCATTGCCC TGCCGCTTGT ACATGCAGCA ACAATATTGT AGATTGCCGT 
GGAAAGGGGC TGACTGAGAT ACCCACCAAC CTCCCGGAGA CAATAACAGA AATGAGACTG 
GAGCAAAATT CGATCAAGGT CATTCCTCCA GGAGCATTCT CACCATATAA AAAGTTGAGA 
AGAATTGATT TAAGCAACAA CCAGATTTCT GAAATAGCCG CAGATGCTTT CCAAGGCCTG 
CGTTCACTCA ACTCCCTTGT TCTCTATGGA AATAAAATCA CAGAGCTCCC TAAAGGCTTG 
TTTGAAGGAC TGGTCTTATT ACAGTTGCTG TTACTCAATG CCAACAAGAT TAATTGTTTA 
CGAGTAGACT CCTTCCAAGA TCTGCACAAC CTGAACCTTC TATCTTTGTA CGACAACAAA 
CTTCAAACCA TCGCCAAAGG CACATTTGCC CCGCTCCGCG CGATCCAGAC GCTGCATTTG 
GCTCAGAACC CGTTTATCTG CGACTGCCAT CTGAAGTGGC TGGCGGATTA TCTCCATACA 
AACCCCATTG AGACCAGTGG TGCCCGCTGC ACCAGCCCCC GCCGACTGGC AAACAAAAGG 
ATTGGGCAGA TCAAAAGCAA GAAATTCCGC TGCTCAGGCA CAGAAGACTA CCGATCCAAG 
CTGAGCGGAG ATTGTTTTGC AGACTTGGCC TGCCCAGAAA AATGTCGCTG TGAAGGAACC 
ACTGTGGATT GTTCCAACCA AAAACTTACC AAGATTCCTG ACCATATTCC CCAGTACACA 
GCTGAGTTGC GCCTCAACAA CAACGAATTC ACAGTTTTGG AGGCAACTGG AATCTTCAAG 
AAACTTCCCC AGCTTAGAAA AATAAACCTG AGCAACAACA AGATAACAGA TATTGAAGAA 
GGGGCTTTCG AAGGTGCCAA TGGCGTGAAT GAGCTACTGC TAACAAGTAA TCGCATGGAA 
AATGTGCGCC ATAAGATGTT CAAGGGCCTG GAAGGATTGA AAACCCTGAT GCTCAGAAGC 
AATCGCATTA GCTGTGTGAA TAATGACAGT TTCACCGGGC TGAGCTCGGT TCGCCTGCTC 
TCCCTCTATG ACAATCAAAT TACTACAGTG GCTCCCGGTG CATTCGACAC GCTGCATTCT 
CTCTCTACAT TGAACCTTTT AGCGAATCCA TTTAATTGCA ACTGCCACCT GGCTTGGTTG 
GGCGACTGGC TTAGGAAGAA GCGCATTGTC ACAGGAAACC CTCGATGCCA AAAGCCCTAT 
TTCCTTAAGG AGATTCCAAT CCAGGATGTG GCTATACAAG ATTTTACTTG TGATGATGGG 
AATGAGGACA ATAGCTGTTC GCCGCTGTCT CGCTGCCCTG CCGAGTGCAC TTGTTTAGAT 
ACTGTTGTTC GATGCAGTAA CAAAGGACTG AAGTCTTTAC CCAAAGGCAT CCCTAAGGAG 
GTCACAGAAC TATATTTGGA TGGAAACATG TTTCCACTTG TTCCAAAGGA ACTTTCTAAC 
TATAAGCACT TGACCCTTAT CGACCTGAGC AACAACCGAA TCAGCACTCT TTCTAACCAC 
AGCTTCAGCA ACATGACCCA GCTCCTCACC CTGATTCTTA GTTACAATCG CCTCCGGTGC 
ATTCCACTCC GGGCATTTGA TGGGTTGAAG TCTCTGAGAC TTTTATCCTT GCACGGCAAT 
GACGTCTCTG CTATACCTGA AGGGGCATTC AATGATCTTT CCTCACTGTC ACATCTAGCT 
ATTGGGGCGA ACCCTCTGTA TTGTGATTGT AACCTGCAGT GGCTTTCCGA CTGGGTGAAG 
TCAGAATATA AAGAACCTGG GATCGCAAGG TGTTCTGGGC CTGGGGAAAT GGCTGATAAG 
CTCCTCCTCA CCACCCCCTC CAAAAAGTTC ACCTGCAATG GGCCTGTAGA TGTCAGCATT 
CTTGCTAAAT GTAACCCCTG CTTATCCAAT CCTTGCAAGA ACGACGGCAC CTGTAACAAC 
GATCCTGTCG AATTCTACCG ATGCACATGT CCCTATGGCT TTAAGGGACA AGACTGCGAT 
GTACCAATCC ATGCCTGCAT CAGTAACCCG TGTCGGAATA GTGGTACCTG CCATTTAAAG 
GAAGGGGACA AAGACGGCTT CTGGTGCGCT TGTGCCGAAG GATTCGAAGG TGAAAACTGC 
GAGGTCAACA TTGACGACTG TGAAGATAAC GACTGCGAGA ATAATTCTAC TTGTGTCGAC 
GGCATTAACA ATTACACGTG CCGCTGCCCA CCGGAATACA CAGCTGCTAA ATTGAATGAG 
GTGGAAAAAG GTGAACTTTG TGAAGAAAAA CTGGATTTCT GCTCCCAAGA TGTCAATCCA 
TGTCAGCATG ACTCAAAATG CATCCTGACA CTGAAAGGCT ACAAATGTGA CTGCACCCCC 
GGATATATTG GGGAACACTG TGATATCGAC TACGATGATT GTCAGGAGAA CAAGTGCAGA 
AATGGAGCCC GTTGCGTTGA TGCAGTGAAT GGTTATACTT GTGTCTGCCC AGAAGGATAC 
AGCGGTTTGT TCTGTGAATC GGCGCCACCA ATGGTTTTAC CTCGCACAAG TCCTTGTGAC 
AACTTTGAAT GTCAAAATGG AGCCCAGTGT ATCGTGAAAG GAAATGAGCC TCAGTGCCAG 
TGCTTGCCCG GATATCACGG AAACAAATGT GAAAAACTAG TCAGTGTAAA TTTTGTGAAC 
AAAGACTCGT TTATCCAAAT TCCTTCGGCA AAGATCCGCA CTCAGGCCAA CATCACTCTG 
CAGGTCGCCA CGGATGAAGA CAGTGGGATC TTACTCTATA AAGGTGACAA GGATCAGGTA 
GCGGTGGAGT TGTATCGTGG GAGAGTGAGA GTTAGTTACG ACACTGGAAT TAACCCTGCA 
TCTGCCATTT ACAGCGTTGA GACTATAAAT GATGGAAATT TCCATGTTGT GGAATTGGTA 
AACCTGGATC AAACACTGTC ACTGGTCGTT GATGGAGGAA CCCCTAAATC CATTACGAAT 
TTACCAAAAC AGTCATTAAT AAGTATTGAC TCCACCCTTT ACATAGGAGG TATGCCCATG 
AAAAATAACA TTGCAGGTCT TCGACAGTCT TCAAGTCAAA ATGGCACCAA CTTCCTAGGC 
TGTATTAGGA ACCTGTACAT CAATAATGAA CTACAGGACT TCAGAAAACT AAATCTCCAA 
GCTGGAGTTC TTCCTGGCTG TGAACCTTGC CATAAAAAAG TCTGTGTCCA TGGCACATGT 
CTGGCTACTA GCCAAGCTGG CTTCACTTGC GAATGCGAAG GAGGATGGAC AGGACAACTG 
TGTGATATCC AGTCCAACGA TCCCTGTCTG GGAAACAAGT GTGTCCATGG CACGTGCCTC 
CCCATTAATT CATTCTCCTA CAGCTGTAAA TGCCTTCAGG GCTTTGTGGG GGTTCTCTGT 
GATGAACCTG AAGATCAGCC CAATCCCTGC CAATCCATTA AATGTAAACA CGGAAAATGC 
AGGCTTTCGG GCTTAGGGAA AGCCTATTGT GAATGCAACA GTGGTTATAC AGGGGACAGC 
TGTGATAAAG CTTTCACTGA TTTATCCCAA CGAATTCCAC AGAAATCTCT TGCCGAGGGG 
AACGGGTCAG AGATTACTAC CAAAGGCAGC AAGGTTATGC TGCTTGCCAG ACGACCGAGA 
AGGTATCAAG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_002936715.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936761.2 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X2, mRNA.

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CloneID	OXa04764
Clone ID Related Accession (Same CDS sequence)	XM_002936715.4 , XM_002936715.5
Accession Version	XM_002936715.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002936715.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GGTGAACTTT GTGAAGAAAA ACTGGATTTC 
TGCTCCCAAG ATGTCAATCC ATGTCAGCAT GACTCAAAAT GCATCCTGAC ACTGAAAGGC 
TACAAATGTG ACTGCACCCC CGGATATATT GGGGAACACT GTGATATCGA CTACGATGAT 
TGTCAGGAGA ACAAGTGCAG AAATGGAGCC CGTTGCGTTG ATGCAGTGAA TGGTTATACT 
TGTGTCTGCC CAGAAGGATA CAGCGGTTTG TTCTGTGAAT CGGCGCCACC AATGGTTTTA 
CCTCGCACAA GTCCTTGTGA CAACTTTGAA TGTCAAAATG GAGCCCAGTG TATCGTGAAA 
GGAAATGAGC CTCAGTGCCA GTGCTTGCCC GGATATCACG GAAACAAATG TGAAAAACTA 
GTCAGTGTAA ATTTTGTGAA CAAAGACTCG TTTATCCAAA TTCCTTCGGC AAAGATCCGC 
ACTCAGGCCA ACATCACTCT GCAGGTCGCC ACGGATGAAG ACAGTGGGAT CTTACTCTAT 
AAAGGTGACA AGGATCAGGT AGCGGTGGAG TTGTATCGTG GGAGAGTGAG AGTTAGTTAC 
GACACTGGAA TTAACCCTGC ATCTGCCATT TACAGCGTTG AGACTATAAA TGATGGAAAT 
TTCCATGTTG TGGAATTGGT AAACCTGGAT CAAACACTGT CACTGGTCGT TGATGGAGGA 
ACCCCTAAAT CCATTACGAA TTTACCAAAA CAGTCATTAA TAAGTATTGA CTCCACCCTT 
TACATAGGAG GTATGCCCAT GAAAAATAAC ATTGCAGGTC TTCGACAGTC TTCAAGTCAA 
AATGGCACCA ACTTCCTAGG CTGTATTAGG AACCTGTACA TCAATAATGA ACTACAGGAC 
TTCAGAAAAC TAAATCTCCA AGCTGGAGTT CTTCCTGGCT GTGAACCTTG CCATAAAAAA 
GTCTGTGTCC ATGGCACATG TCTGGCTACT AGCCAAGCTG GCTTCACTTG CGAATGCGAA 
GGAGGATGGA CAGGACAACT GTGTGATATC CAGTCCAACG ATCCCTGTCT GGGAAACAAG 
TGTGTCCATG GCACGTGCCT CCCCATTAAT TCATTCTCCT ACAGCTGTAA ATGCCTTCAG 
GGCTTTGTGG GGGTTCTCTG TGATGAACCT GAAGATCAGC CCAATCCCTG CCAATCCATT 
AAATGTAAAC ACGGAAAATG CAGGCTTTCG GGCTTAGGGA AAGCCTATTG TGAATGCAAC 
AGTGGTTATA CAGGGGACAG CTGTGATAAA GAAATCTCTT GCCGAGGGGA ACGGGTCAGA 
GATTACTACC AAAGGCAGCA AGGTTATGCT GCTTGCCAGA CGACCGAGAA GGTATCAAGA 
CTAGAATGCA AAGGAGGATG CTCAAATGGT CAGTGCTGTG GGCCACTGAG GAGCAAAAGG 
CGGAAATATG CTTTTGAATG CACAGATGGT TCATCCTTTG TGGACGAGGT GGAGAAAGTC 
ATAAAGTGCG GTTGTACAAA GTGCCCCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936761.2
CDS	758..5350
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKEISCRGERVRDYYQRQQGYAACQTTEKVSRLECKGGCSNGQCCGPLRSKRRKYAFECTDGSSFVDEVEKVIKCGCTKCPS

Target ORF information:

RefSeq Version	XM_002936715.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936715.4

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GGTGAACTTT GTGAAGAAAA ACTGGATTTC 
TGCTCCCAAG ATGTCAATCC ATGTCAGCAT GACTCAAAAT GCATCCTGAC ACTGAAAGGC 
TACAAATGTG ACTGCACCCC CGGATATATT GGGGAACACT GTGATATCGA CTACGATGAT 
TGTCAGGAGA ACAAGTGCAG AAATGGAGCC CGTTGCGTTG ATGCAGTGAA TGGTTATACT 
TGTGTCTGCC CAGAAGGATA CAGCGGTTTG TTCTGTGAAT CGGCGCCACC AATGGTTTTA 
CCTCGCACAA GTCCTTGTGA CAACTTTGAA TGTCAAAATG GAGCCCAGTG TATCGTGAAA 
GGAAATGAGC CTCAGTGCCA GTGCTTGCCC GGATATCACG GAAACAAATG TGAAAAACTA 
GTCAGTGTAA ATTTTGTGAA CAAAGACTCG TTTATCCAAA TTCCTTCGGC AAAGATCCGC 
ACTCAGGCCA ACATCACTCT GCAGGTCGCC ACGGATGAAG ACAGTGGGAT CTTACTCTAT 
AAAGGTGACA AGGATCAGGT AGCGGTGGAG TTGTATCGTG GGAGAGTGAG AGTTAGTTAC 
GACACTGGAA TTAACCCTGC ATCTGCCATT TACAGCGTTG AGACTATAAA TGATGGAAAT 
TTCCATGTTG TGGAATTGGT AAACCTGGAT CAAACACTGT CACTGGTCGT TGATGGAGGA 
ACCCCTAAAT CCATTACGAA TTTACCAAAA CAGTCATTAA TAAGTATTGA CTCCACCCTT 
TACATAGGAG GTATGCCCAT GAAAAATAAC ATTGCAGGTC TTCGACAGTC TTCAAGTCAA 
AATGGCACCA ACTTCCTAGG CTGTATTAGG AACCTGTACA TCAATAATGA ACTACAGGAC 
TTCAGAAAAC TAAATCTCCA AGCTGGAGTT CTTCCTGGCT GTGAACCTTG CCATAAAAAA 
GTCTGTGTCC ATGGCACATG TCTGGCTACT AGCCAAGCTG GCTTCACTTG CGAATGCGAA 
GGAGGATGGA CAGGACAACT GTGTGATATC CAGTCCAACG ATCCCTGTCT GGGAAACAAG 
TGTGTCCATG GCACGTGCCT CCCCATTAAT TCATTCTCCT ACAGCTGTAA ATGCCTTCAG 
GGCTTTGTGG GGGTTCTCTG TGATGAACCT GAAGATCAGC CCAATCCCTG CCAATCCATT 
AAATGTAAAC ACGGAAAATG CAGGCTTTCG GGCTTAGGGA AAGCCTATTG TGAATGCAAC 
AGTGGTTATA CAGGGGACAG CTGTGATAAA GAAATCTCTT GCCGAGGGGA ACGGGTCAGA 
GATTACTACC AAAGGCAGCA AGGTTATGCT GCTTGCCAGA CGACCGAGAA GGTATCAAGA 
CTAGAATGCA AAGGAGGATG CTCAAATGGT CAGTGCTGTG GGCCACTGAG GAGCAAAAGG 
CGGAAATATG CTTTTGAATG CACAGATGGT TCATCCTTTG TGGACGAGGT GGAGAAAGTC 
ATAAAGTGCG GTTGTACAAA GTGCCCCTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_012955706.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811160.1 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa35231
Clone ID Related Accession (Same CDS sequence)	XM_012955706.2 , XM_012955706.3
Accession Version	XM_012955706.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GCTGCTAAAT TGAATGAGGT GGAAAAAGGT 
GAACTTTGTG AAGAAAAACT GGATTTCTGC TCCCAAGATG TCAATCCATG TCAGCATGAC 
TCAAAATGCA TCCTGACACT GAAAGGCTAC AAATGTGACT GCACCCCCGG ATATATTGGG 
GAACACTGTG ATATCGACTA CGATGATTGT CAGGAGAACA AGTGCAGAAA TGGAGCCCGT 
TGCGTTGATG CAGTGAATGG TTATACTTGT GTCTGCCCAG AAGGATACAG CGGTTTGTTC 
TGTGAATCGG CGCCACCAAT GGTTTTACCT CGCACAAGTC CTTGTGACAA CTTTGAATGT 
CAAAATGGAG CCCAGTGTAT CGTGAAAGGA AATGAGCCTC AGTGCCAGTG CTTGCCCGGA 
TATCACGGAA ACAAATGTGA AAAACTAGTC AGTGTAAATT TTGTGAACAA AGACTCGTTT 
ATCCAAATTC CTTCGGCAAA GATCCGCACT CAGGCCAACA TCACTCTGCA GGTCGCCACG 
GATGAAGACA GTGGGATCTT ACTCTATAAA GGTGACAAGG ATCAGGTAGC GGTGGAGTTG 
TATCGTGGGA GAGTGAGAGT TAGTTACGAC ACTGGAATTA ACCCTGCATC TGCCATTTAC 
AGCGTTGAGA CTATAAATGA TGGAAATTTC CATGTTGTGG AATTGGTAAA CCTGGATCAA 
ACACTGTCAC TGGTCGTTGA TGGAGGAACC CCTAAATCCA TTACGAATTT ACCAAAACAG 
TCATTAATAA GTATTGACTC CACCCTTTAC ATAGGAGGTA TGCCCATGAA AAATAACATT 
GCAGGTCTTC GACAGTCTTC AAGTCAAAAT GGCACCAACT TCCTAGGCTG TATTAGGAAC 
CTGTACATCA ATAATGAACT ACAGGACTTC AGAAAACTAA ATCTCCAAGC TGGAGTTCTT 
CCTGGCTGTG AACCTTGCCA TAAAAAAGTC TGTGTCCATG GCACATGTCT GGCTACTAGC 
CAAGCTGGCT TCACTTGCGA ATGCGAAGGA GGATGGACAG GACAACTGTG TGATATCCAG 
TCCAACGATC CCTGTCTGGG AAACAAGTGT GTCCATGGCA CGTGCCTCCC CATTAATTCA 
TTCTCCTACA GCTGTAAATG CCTTCAGGGC TTTGTGGGGG TTCTCTGTGA TGAACCTGAA 
GATCAGCCCA ATCCCTGCCA ATCCATTAAA TGTAAACACG GAAAATGCAG GCTTTCGGGC 
TTAGGGAAAG CCTATTGTGA ATGCAACAGT GGTTATACAG GGGACAGCTG TGATAAAGAA 
ATCTCTTGCC GAGGGGAACG GGTCAGAGAT TACTACCAAA GGCAGCAAGG TTATGCTGCT 
TGCCAGACGA CCGAGAAGGT ATCAAGACTA GAATGCAAAG GAGGATGCTC AAATGGTCAG 
TGCTGTGGGC CACTGAGGAG CAAAAGGCGG AAATATGCTT TTGAATGCAC AGATGGTTCA 
TCCTTTGTGG ACGAGGTGGA GAAAGTCATA AAGTGCGGTT GTACAAAGTG CCCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811160.1
CDS	759..5378
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKEISCRGERVRDYYQRQQGYAACQTTEKVSRLECKGGCSNGQCCGPLRSKRRKYAFECTDGSSFVDEVEKVIKCGCTKCPS

Target ORF information:

RefSeq Version	XM_012955706.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955706.2

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GCTGCTAAAT TGAATGAGGT GGAAAAAGGT 
GAACTTTGTG AAGAAAAACT GGATTTCTGC TCCCAAGATG TCAATCCATG TCAGCATGAC 
TCAAAATGCA TCCTGACACT GAAAGGCTAC AAATGTGACT GCACCCCCGG ATATATTGGG 
GAACACTGTG ATATCGACTA CGATGATTGT CAGGAGAACA AGTGCAGAAA TGGAGCCCGT 
TGCGTTGATG CAGTGAATGG TTATACTTGT GTCTGCCCAG AAGGATACAG CGGTTTGTTC 
TGTGAATCGG CGCCACCAAT GGTTTTACCT CGCACAAGTC CTTGTGACAA CTTTGAATGT 
CAAAATGGAG CCCAGTGTAT CGTGAAAGGA AATGAGCCTC AGTGCCAGTG CTTGCCCGGA 
TATCACGGAA ACAAATGTGA AAAACTAGTC AGTGTAAATT TTGTGAACAA AGACTCGTTT 
ATCCAAATTC CTTCGGCAAA GATCCGCACT CAGGCCAACA TCACTCTGCA GGTCGCCACG 
GATGAAGACA GTGGGATCTT ACTCTATAAA GGTGACAAGG ATCAGGTAGC GGTGGAGTTG 
TATCGTGGGA GAGTGAGAGT TAGTTACGAC ACTGGAATTA ACCCTGCATC TGCCATTTAC 
AGCGTTGAGA CTATAAATGA TGGAAATTTC CATGTTGTGG AATTGGTAAA CCTGGATCAA 
ACACTGTCAC TGGTCGTTGA TGGAGGAACC CCTAAATCCA TTACGAATTT ACCAAAACAG 
TCATTAATAA GTATTGACTC CACCCTTTAC ATAGGAGGTA TGCCCATGAA AAATAACATT 
GCAGGTCTTC GACAGTCTTC AAGTCAAAAT GGCACCAACT TCCTAGGCTG TATTAGGAAC 
CTGTACATCA ATAATGAACT ACAGGACTTC AGAAAACTAA ATCTCCAAGC TGGAGTTCTT 
CCTGGCTGTG AACCTTGCCA TAAAAAAGTC TGTGTCCATG GCACATGTCT GGCTACTAGC 
CAAGCTGGCT TCACTTGCGA ATGCGAAGGA GGATGGACAG GACAACTGTG TGATATCCAG 
TCCAACGATC CCTGTCTGGG AAACAAGTGT GTCCATGGCA CGTGCCTCCC CATTAATTCA 
TTCTCCTACA GCTGTAAATG CCTTCAGGGC TTTGTGGGGG TTCTCTGTGA TGAACCTGAA 
GATCAGCCCA ATCCCTGCCA ATCCATTAAA TGTAAACACG GAAAATGCAG GCTTTCGGGC 
TTAGGGAAAG CCTATTGTGA ATGCAACAGT GGTTATACAG GGGACAGCTG TGATAAAGAA 
ATCTCTTGCC GAGGGGAACG GGTCAGAGAT TACTACCAAA GGCAGCAAGG TTATGCTGCT 
TGCCAGACGA CCGAGAAGGT ATCAAGACTA GAATGCAAAG GAGGATGCTC AAATGGTCAG 
TGCTGTGGGC CACTGAGGAG CAAAAGGCGG AAATATGCTT TTGAATGCAC AGATGGTTCA 
TCCTTTGTGG ACGAGGTGGA GAAAGTCATA AAGTGCGGTT GTACAAAGTG CCCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

slit2 ( XM_012955707.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012811161.1 Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X3, mRNA.

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CloneID	OXa35232
Clone ID Related Accession (Same CDS sequence)	XM_012955707.2
Accession Version	XM_012955707.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012955707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GCTGCTAAAT TGAATGAGGT GGAAAAAGGT 
GAACTTTGTG AAGAAAAACT GGATTTCTGC TCCCAAGATG TCAATCCATG TCAGCATGAC 
TCAAAATGCA TCCTGACACT GAAAGGCTAC AAATGTGACT GCACCCCCGG ATATATTGGG 
GAACACTGTG ATATCGACTA CGATGATTGT CAGGAGAACA AGTGCAGAAA TGGAGCCCGT 
TGCGTTGATG CAGTGAATGG TTATACTTGT GTCTGCCCAG AAGGATACAG CGGTTTGTTC 
TGTGAATCGG CGCCACCAAT GGTTTTACCT CGCACAAGTC CTTGTGACAA CTTTGAATGT 
CAAAATGGAG CCCAGTGTAT CGTGAAAGGA AATGAGCCTC AGTGCCAGTG CTTGCCCGGA 
TATCACGGAA ACAAATGTGA AAAACTAGTC AGTGTAAATT TTGTGAACAA AGACTCGTTT 
ATCCAAATTC CTTCGGCAAA GATCCGCACT CAGGCCAACA TCACTCTGCA GGTCGCCACG 
GATGAAGACA GTGGGATCTT ACTCTATAAA GGTGACAAGG ATCAGGTAGC GGTGGAGTTG 
TATCGTGGGA GAGTGAGAGT TAGTTACGAC ACTGGAATTA ACCCTGCATC TGCCATTTAC 
AGCGTTGAGA CTATAAATGA TGGAAATTTC CATGTTGTGG AATTGGTAAA CCTGGATCAA 
ACACTGTCAC TGGTCGTTGA TGGAGGAACC CCTAAATCCA TTACGAATTT ACCAAAACAG 
TCATTAATAA GTATTGACTC CACCCTTTAC ATAGGAGGTA TGCCCATGAA AAATAACATT 
GCAGGTCTTC GACAGTCTTC AAGTCAAAAT GGCACCAACT TCCTAGGCTG TATTAGGAAC 
CTGTACATCA ATAATGAACT ACAGGACTTC AGAAAACTAA ATCTCCAAGC TGGAGTTCTT 
CCTGGCTGTG AACCTTGCCA TAAAAAAGTC TGTGTCCATG GCACATGTCT GGCTACTAGC 
CAAGCTGGCT TCACTTGCGA ATGCGAAGGA GGATGGACAG GACAACTGTG TGATATCCAG 
TCCAACGATC CCTGTCTGGG AAACAAGTGT GTCCATGGCA CGTGCCTCCC CATTAATTCA 
TTCTCCTACA GCTGTAAATG CCTTCAGGGC TTTGTGGGGG TTCTCTGTGA TGAACCTGAA 
GATCAGCCCA ATCCCTGCCA ATCCATTAAA TGTAAACACG GAAAATGCAG GCTTTCGGGC 
TTAGGGAAAG CCTATTGTGA ATGCAACAGT GGTTATACAG GGGACAGCTG TGATAAAGCT 
TTCACTGATT TATCCCAACG AATTCCACAG AAATCTCTTG CCGAGGGGAA CGGGTCAGAG 
ATTACTACCA AAGGCAGCAA GGTTATGCTG CTTGCCAGAC GACCGAGAAG GTATCAAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012811161.1
CDS	763..5265
Translation	MLPSVVKKKMCYIGFWKLSVSLGLVLVTLSEVAPQPCPAQCSCTGTTVDCHGLTLRSVPRNIPRNTERLDLNANNITRISKTDFAGLRHLRILQLMENKITTIERGAFQDLKELERLRLNRNNLQVFPELLFLGTPKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPIHLRGHNIAEVQKREFVCTGQQSFMVHSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEMRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISEIAADAFQGLRSLNSLVLYGNKITELPKGLFEGLVLLQLLLLNANKINCLRVDSFQDLHNLNLLSLYDNKLQTIAKGTFAPLRAIQTLHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINLSNNKITDIEEGAFEGANGVNELLLTSNRMENVRHKMFKGLEGLKTLMLRSNRISCVNNDSFTGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCHLAWLGDWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNEDNSCSPLSRCPAECTCLDTVVRCSNKGLKSLPKGIPKEVTELYLDGNMFPLVPKELSNYKHLTLIDLSNNRISTLSNHSFSNMTQLLTLILSYNRLRCIPLRAFDGLKSLRLLSLHGNDVSAIPEGAFNDLSSLSHLAIGANPLYCDCNLQWLSDWVKSEYKEPGIARCSGPGEMADKLLLTTPSKKFTCNGPVDVSILAKCNPCLSNPCKNDGTCNNDPVEFYRCTCPYGFKGQDCDVPIHACISNPCRNSGTCHLKEGDKDGFWCACAEGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCRCPPEYTAAKLNEVEKGELCEEKLDFCSQDVNPCQHDSKCILTLKGYKCDCTPGYIGEHCDIDYDDCQENKCRNGARCVDAVNGYTCVCPEGYSGLFCESAPPMVLPRTSPCDNFECQNGAQCIVKGNEPQCQCLPGYHGNKCEKLVSVNFVNKDSFIQIPSAKIRTQANITLQVATDEDSGILLYKGDKDQVAVELYRGRVRVSYDTGINPASAIYSVETINDGNFHVVELVNLDQTLSLVVDGGTPKSITNLPKQSLISIDSTLYIGGMPMKNNIAGLRQSSSQNGTNFLGCIRNLYINNELQDFRKLNLQAGVLPGCEPCHKKVCVHGTCLATSQAGFTCECEGGWTGQLCDIQSNDPCLGNKCVHGTCLPINSFSYSCKCLQGFVGVLCDEPEDQPNPCQSIKCKHGKCRLSGLGKAYCECNSGYTGDSCDKAFTDLSQRIPQKSLAEGNGSEITTKGSKVMLLARRPRRYQD

Target ORF information:

RefSeq Version	XM_012955707.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis slit guidance ligand 2 (slit2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012955707.2

ORF Insert Sequence:

ATGTTGCCAA GTGTTGTGAA GAAGAAGATG TGCTATATTG GGTTCTGGAA GCTGTCCGTC 
TCTTTGGGTT TAGTACTGGT CACACTAAGC GAGGTGGCAC CTCAGCCTTG CCCAGCTCAG 
TGCTCTTGTA CGGGCACCAC CGTGGACTGC CATGGGCTTA CCCTGCGTAG CGTGCCCCGC 
AACATCCCAA GGAACACCGA GCGCCTGGAC CTGAATGCCA ATAATATCAC TAGAATCTCC 
AAGACAGACT TTGCTGGATT AAGACACCTC CGCATCTTGC AGCTTATGGA GAACAAGATT 
ACCACAATTG AAAGGGGAGC ATTCCAAGAC CTGAAAGAAC TGGAAAGACT GCGCCTTAAC 
CGAAACAATC TGCAGGTGTT CCCTGAACTG CTTTTCCTGG GGACTCCAAA GCTTTACAGA 
CTTGACCTCA GCGAGAACCA GATCCAAGCC ATCCCGAGAA AGGCATTTCG TGGTGCAGTA 
GATATTAAAA ATCTGCAGCT GGATTATAAC CAGATCAGCT GTATTGAAGA TGGGGCATTC 
CGAGCACTGC GCGACCTGGA AGTACTCACC CTCAACAATA ACAATATTAC GCGACTTTCC 
GTGGCGAGCT TCAACCACAT GCCTAAACTC AGGACCTTTC GACTCCATTC CAACAACTTG 
TACTGTGACT GTCACCTCGC TTGGTTATCC GACTGGCTGC GCCAGAGGCC CCGAGTGGGT 
CTCTACACCC AGTGCATGGG ACCCATTCAC CTGAGAGGGC ATAACATTGC TGAGGTTCAG 
AAGCGAGAGT TCGTCTGCAC TGGTCAGCAG TCATTTATGG TCCATTCTTG TAGCGTCTTG 
CATTGCCCTG CCGCTTGTAC ATGCAGCAAC AATATTGTAG ATTGCCGTGG AAAGGGGCTG 
ACTGAGATAC CCACCAACCT CCCGGAGACA ATAACAGAAA TGAGACTGGA GCAAAATTCG 
ATCAAGGTCA TTCCTCCAGG AGCATTCTCA CCATATAAAA AGTTGAGAAG AATTGATTTA 
AGCAACAACC AGATTTCTGA AATAGCCGCA GATGCTTTCC AAGGCCTGCG TTCACTCAAC 
TCCCTTGTTC TCTATGGAAA TAAAATCACA GAGCTCCCTA AAGGCTTGTT TGAAGGACTG 
GTCTTATTAC AGTTGCTGTT ACTCAATGCC AACAAGATTA ATTGTTTACG AGTAGACTCC 
TTCCAAGATC TGCACAACCT GAACCTTCTA TCTTTGTACG ACAACAAACT TCAAACCATC 
GCCAAAGGCA CATTTGCCCC GCTCCGCGCG ATCCAGACGC TGCATTTGGC TCAGAACCCG 
TTTATCTGCG ACTGCCATCT GAAGTGGCTG GCGGATTATC TCCATACAAA CCCCATTGAG 
ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC CGACTGGCAA ACAAAAGGAT TGGGCAGATC 
AAAAGCAAGA AATTCCGCTG CTCAGGCACA GAAGACTACC GATCCAAGCT GAGCGGAGAT 
TGTTTTGCAG ACTTGGCCTG CCCAGAAAAA TGTCGCTGTG AAGGAACCAC TGTGGATTGT 
TCCAACCAAA AACTTACCAA GATTCCTGAC CATATTCCCC AGTACACAGC TGAGTTGCGC 
CTCAACAACA ACGAATTCAC AGTTTTGGAG GCAACTGGAA TCTTCAAGAA ACTTCCCCAG 
CTTAGAAAAA TAAACCTGAG CAACAACAAG ATAACAGATA TTGAAGAAGG GGCTTTCGAA 
GGTGCCAATG GCGTGAATGA GCTACTGCTA ACAAGTAATC GCATGGAAAA TGTGCGCCAT 
AAGATGTTCA AGGGCCTGGA AGGATTGAAA ACCCTGATGC TCAGAAGCAA TCGCATTAGC 
TGTGTGAATA ATGACAGTTT CACCGGGCTG AGCTCGGTTC GCCTGCTCTC CCTCTATGAC 
AATCAAATTA CTACAGTGGC TCCCGGTGCA TTCGACACGC TGCATTCTCT CTCTACATTG 
AACCTTTTAG CGAATCCATT TAATTGCAAC TGCCACCTGG CTTGGTTGGG CGACTGGCTT 
AGGAAGAAGC GCATTGTCAC AGGAAACCCT CGATGCCAAA AGCCCTATTT CCTTAAGGAG 
ATTCCAATCC AGGATGTGGC TATACAAGAT TTTACTTGTG ATGATGGGAA TGAGGACAAT 
AGCTGTTCGC CGCTGTCTCG CTGCCCTGCC GAGTGCACTT GTTTAGATAC TGTTGTTCGA 
TGCAGTAACA AAGGACTGAA GTCTTTACCC AAAGGCATCC CTAAGGAGGT CACAGAACTA 
TATTTGGATG GAAACATGTT TCCACTTGTT CCAAAGGAAC TTTCTAACTA TAAGCACTTG 
ACCCTTATCG ACCTGAGCAA CAACCGAATC AGCACTCTTT CTAACCACAG CTTCAGCAAC 
ATGACCCAGC TCCTCACCCT GATTCTTAGT TACAATCGCC TCCGGTGCAT TCCACTCCGG 
GCATTTGATG GGTTGAAGTC TCTGAGACTT TTATCCTTGC ACGGCAATGA CGTCTCTGCT 
ATACCTGAAG GGGCATTCAA TGATCTTTCC TCACTGTCAC ATCTAGCTAT TGGGGCGAAC 
CCTCTGTATT GTGATTGTAA CCTGCAGTGG CTTTCCGACT GGGTGAAGTC AGAATATAAA 
GAACCTGGGA TCGCAAGGTG TTCTGGGCCT GGGGAAATGG CTGATAAGCT CCTCCTCACC 
ACCCCCTCCA AAAAGTTCAC CTGCAATGGG CCTGTAGATG TCAGCATTCT TGCTAAATGT 
AACCCCTGCT TATCCAATCC TTGCAAGAAC GACGGCACCT GTAACAACGA TCCTGTCGAA 
TTCTACCGAT GCACATGTCC CTATGGCTTT AAGGGACAAG ACTGCGATGT ACCAATCCAT 
GCCTGCATCA GTAACCCGTG TCGGAATAGT GGTACCTGCC ATTTAAAGGA AGGGGACAAA 
GACGGCTTCT GGTGCGCTTG TGCCGAAGGA TTCGAAGGTG AAAACTGCGA GGTCAACATT 
GACGACTGTG AAGATAACGA CTGCGAGAAT AATTCTACTT GTGTCGACGG CATTAACAAT 
TACACGTGCC GCTGCCCACC GGAATACACA GCTGCTAAAT TGAATGAGGT GGAAAAAGGT 
GAACTTTGTG AAGAAAAACT GGATTTCTGC TCCCAAGATG TCAATCCATG TCAGCATGAC 
TCAAAATGCA TCCTGACACT GAAAGGCTAC AAATGTGACT GCACCCCCGG ATATATTGGG 
GAACACTGTG ATATCGACTA CGATGATTGT CAGGAGAACA AGTGCAGAAA TGGAGCCCGT 
TGCGTTGATG CAGTGAATGG TTATACTTGT GTCTGCCCAG AAGGATACAG CGGTTTGTTC 
TGTGAATCGG CGCCACCAAT GGTTTTACCT CGCACAAGTC CTTGTGACAA CTTTGAATGT 
CAAAATGGAG CCCAGTGTAT CGTGAAAGGA AATGAGCCTC AGTGCCAGTG CTTGCCCGGA 
TATCACGGAA ACAAATGTGA AAAACTAGTC AGTGTAAATT TTGTGAACAA AGACTCGTTT 
ATCCAAATTC CTTCGGCAAA GATCCGCACT CAGGCCAACA TCACTCTGCA GGTCGCCACG 
GATGAAGACA GTGGGATCTT ACTCTATAAA GGTGACAAGG ATCAGGTAGC GGTGGAGTTG 
TATCGTGGGA GAGTGAGAGT TAGTTACGAC ACTGGAATTA ACCCTGCATC TGCCATTTAC 
AGCGTTGAGA CTATAAATGA TGGAAATTTC CATGTTGTGG AATTGGTAAA CCTGGATCAA 
ACACTGTCAC TGGTCGTTGA TGGAGGAACC CCTAAATCCA TTACGAATTT ACCAAAACAG 
TCATTAATAA GTATTGACTC CACCCTTTAC ATAGGAGGTA TGCCCATGAA AAATAACATT 
GCAGGTCTTC GACAGTCTTC AAGTCAAAAT GGCACCAACT TCCTAGGCTG TATTAGGAAC 
CTGTACATCA ATAATGAACT ACAGGACTTC AGAAAACTAA ATCTCCAAGC TGGAGTTCTT 
CCTGGCTGTG AACCTTGCCA TAAAAAAGTC TGTGTCCATG GCACATGTCT GGCTACTAGC 
CAAGCTGGCT TCACTTGCGA ATGCGAAGGA GGATGGACAG GACAACTGTG TGATATCCAG 
TCCAACGATC CCTGTCTGGG AAACAAGTGT GTCCATGGCA CGTGCCTCCC CATTAATTCA 
TTCTCCTACA GCTGTAAATG CCTTCAGGGC TTTGTGGGGG TTCTCTGTGA TGAACCTGAA 
GATCAGCCCA ATCCCTGCCA ATCCATTAAA TGTAAACACG GAAAATGCAG GCTTTCGGGC 
TTAGGGAAAG CCTATTGTGA ATGCAACAGT GGTTATACAG GGGACAGCTG TGATAAAGCT 
TTCACTGATT TATCCCAACG AATTCCACAG AAATCTCTTG CCGAGGGGAA CGGGTCAGAG 
ATTACTACCA AAGGCAGCAA GGTTATGCTG CTTGCCAGAC GACCGAGAAG GTATCAAGAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

slit2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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