SLIT2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014112675.2	XP_013968150.1	slit homolog 2 protein isoform X6
XM_014112671.1	XP_013968146.1	slit homolog 2 protein isoform X1
XM_014112673.1	XP_013968148.1	slit homolog 2 protein isoform X3
XM_014112675.1	XP_013968150.1	slit homolog 2 protein isoform X5
XM_014112672.2	XP_013968147.1	slit homolog 2 protein isoform X3
XM_014112673.2	XP_013968148.1	slit homolog 2 protein isoform X4
XM_014112672.1	XP_013968147.1	slit homolog 2 protein isoform X2
XM_014112674.1	XP_013968149.1	slit homolog 2 protein isoform X4
XM_014112671.2	XP_013968146.1	slit homolog 2 protein isoform X1
XM_014112674.2	XP_013968149.1	slit homolog 2 protein isoform X5
XM_022417277.1	XP_022272985.1	slit homolog 2 protein isoform X7
XM_022417276.1	XP_022272984.1	slit homolog 2 protein isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe287416	XM_022417277.1 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe287415	XM_022417276.1 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35885	XM_014112671.2 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35886	XM_014112672.2 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35887	XM_014112673.2 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35888	XM_014112674.2 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35889	XM_014112675.2 Latest version!	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
View Old Accession Versions >>
OCe35889	XM_014112675.1	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35887	XM_014112673.1	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35886	XM_014112672.1	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35885	XM_014112671.1	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe35888	XM_014112674.1	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

SLIT2 ( XM_022417277.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272985.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe287416
Clone ID Related Accession (Same CDS sequence)	XM_022417277.1
Accession Version	XM_022417277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT TTTCATCAGC TCTGCACGCG TGTGATCTCA GTGAAAACCA AATTCAGGCA 
ATTCCAAGGA AGGCTTTCCG TGGGGCAGTT GACATTAAAA ATTTGCAACT GGATTACAAC 
CAGATCAGCT GTATTGAAGA TGGGGCATTT AGAGCTCTGC GGGACCTGGA AGTGCTCACT 
CTCAACAATA ACAACATTAC TAGACTTTCT GTGGCAAGTT TCAACCATAT GCCTAAACTT 
AGGACTTTTC GGCTGCATTC AAACAATCTG TATTGCGACT GCCACCTGGC CTGGCTTTCT 
GACTGGCTGC GCCAAAGGCC CCGGGTTGGT CTCTACACTC AGTGTATGGG CCCATCCCAC 
CTGAGGGGTC ATAACGTAGC CGAGGTTCAA AAACGCGAAT TTGTCTGCAG TGATGAGGAA 
GAAGGCCACC AGTCCTTCAT GGCTCCTTCT TGCAGCGTCC TGCATTGTCC AGCCGCTTGT 
ACCTGTAGCA ACAATATCGT AGACTGTCGT GGGAAAGGTC TCACTGAGAT CCCCACGAAC 
CTGCCAGAGA CCATCACAGA AATACGTTTG GAACAGAACT CAATCAAGGT CATCCCTCCT 
GGAGCTTTCT CACCATATAA AAAGCTTAGA AGAATTGACC TGAGCAATAA TCAGATCTCT 
GAACTAGCAC CGGACGCTTT CCAAGGACTA CGCTCTCTGA ATTCACTTGT CCTCTATGGA 
AATAAAATCA CGGAACTCCC AAAAAGTTTA TTTGAAGGAC TGTTTTCCTT ACAGCTGCTA 
TTATTGAATG CCAACAAGAT AAACTGCCTT CGGGTAGATG CTTTTCAGGA TCTGCACAAC 
CTGAATCTTC TCTCCCTGTA CGACAACAAG CTGCAGACCA TCGCCAAGGG GACCTTCTCA 
CCTCTCCGGG CCATTCAGAC CATGCACCTG GCCCAGAACC CCTTTATTTG TGACTGCCAT 
CTCAAGTGGC TGGCGGACTA TCTCCACACC AACCCCATCG AGACCAGTGG TGCCCGGTGC 
ACCAGCCCCC GGCGCCTGGC AAACAAAAGA ATCGGACAGA TCAAAAGCAA GAAATTCCGT 
TGTTCAGCTA AAGAACAGTA TTTCATTCCA GGTACAGAAG ATTATCGATC AAAATTAAGC 
GGGGACTGCT TTGCAGATCT GGCTTGCCCT GAAAAGTGCC GCTGTGAAGG AACCACAGTA 
GATTGCTCCA ATCAAAAACT CACCAAAATC CCAGACCACA TCCCCCAGTA CACTGCAGAG 
CTGCGTCTCA ATAATAATGA ATTCACAGTG CTGGAAGCTA CAGGAATCTT CAAGAAACTT 
CCGCAGTTAC GTAAAATAAA CTTCAGCAAC AACAAGATCA CAGACATTGA AGAAGGAGCA 
TTTGAAGGAG CAGCTGGTGT AAACGAAATC CTTCTCACGA GTAACCGTTT GGAAAATGTT 
CAGCATAAGA TGTTCAAGGG ATTGGAAAGC CTGAAAACGT TGATGTTGCG AAGCAATCGC 
ATAAGCTGCG TTGGCAACGA TAGCTTCATA GGCCTGAGCT CTGTGCGGTT GCTTTCGCTG 
TACGATAATC AGATCGCCAC CATCGCGCCG GGGGCGTTCG ACACCCTGCA CTCGTTGTCC 
ACCCTAAACC TGTTGGCCAA CCCTTTTAAC TGCAACTGCT ACCTGGCTTG GCTGGGCGAG 
TGGCTCAGGA AGAAAAGAAT TGTAACCGGA AATCCTCGCT GTCAAAAACC ATACTTCCTC 
AAAGAAATCC CCATCCAGGA CGTCGCCATT CAAGACTTCA CGTGTGACGA CGGAAATGAC 
GACAGTAGCT GTTCTCCACT CTCGCGCTGT CCCACGGAAT GCACGTGCTT GGATACAGTT 
GTCCGATGTA GCAACAAGGG CCTGAAGGTC TTGCCCAAAG GTATTCCCAG AGACGTCACT 
GAACTGTATC TGGATGGGAA CCACTTTACC TTGGTTCCCA AGGAGCTCTA TAACTACAAA 
CATCTAACGC TTATAGACCT GAGCAACAAC CGCATAAGCA CTCTTTCTAA TCAGAGCTTC 
AGCAACATGA CCCAGCTCCT CACCCTAATT CTCAGTTACA ACCGTTTGAG ATGTATTCCT 
CCTCGAACCT TCGATGGACT CAAGTCTCTC CGATTACTTT CATTACATGG AAATGACATT 
TCTGTTGTGC CTGAAGGTGC TTTCAGTGAT CTCTCTGCAT TATCACACCT AGCAATCGGA 
GCCAACCCCC TTTACTGTGA TTGCAACATG CAGTGGTTAT CGGACTGGGT AAAGTCGGAA 
TACAAAGAAC CCGGGATTGC TCGCTGTGCC GGCCCCGGAG AAATGGCAGA TAAATTATTA 
CTCACGACTC CCTCCAAAAA ATTTACATGT CAAGGTCCTG TGGATATCAA TATTCTAGCT 
AAATGTAATC CCTGCTTATC AAACCCATGT AAGAATGATG GCACCTGTAA CAATGATCCA 
GTCGACTTTT ATCGCTGTAC CTGTCCGTAT GGTTTCAAGG GGCAGGACTG TGATGTCCCA 
ATCCACGCAT GCATCAGTAA CCCGTGTACA CATGGAGGAA CTTGCCACTT AAAGGAGGGA 
GAAAAAGATG GATTCTGGTG TATTTGTGCC GATGGATTTG AAGGAGAAAA TTGTGAAGTC 
AATGTTGATG ACTGTGAAGA TAATGACTGT GAAAATAACT CTACGTGTGT CGATGGAATT 
AATAACTACA CATGCCTTTG TCCGCCTGAG TACACAGGCG AGTTGTGTGA GGAGAAGCTG 
GACTTCTGCG CTCAGGACCT GAACCCCTGC CAGCACGACT CCAAGTGCAT CCTGATGCCC 
AAAGGATTCA AATGCGACTG CACGCCGGGG TACGTGGGCG AGCACTGCGA CATCGACTTC 
GACGACTGCC AGGATCACAA GTGTAAAAAC GGAGCGCACT GCACGGACGC GGTGAACGGC 
TACACGTGCA CCTGCCCCGA AGGCTACAGC GGCTTGTTCT GTGAATTCTC CCCGCCCATG 
GTCCTCCCAC GCACCAGCCC CTGTGACAAC TTCGACTGTC AGAACGGGGC GCAGTGCATC 
GTCAGGGCGG GCGAGCCAAT CTGCCAGTGT CTGCCCGGCT ACCAGGGGGA CAAGTGTGAG 
AAGTTGGTCA GCGTGAACTT CGTGAACAAA GAGTCGTATC TTCAAATTCC TTCAGCCAAG 
GTCCGGCCCC AAACGAACAT CACCCTGCAG ATTGCCACCG ACGAAGACAG CGGGATCCTC 
CTGTACAAGG GCGACAAGGA CCACATTGCC GTGGAGCTGT ATCGGGGACG GGTGCGCGCC 
AGCTACGACA CCGGCTCGCA CCCCGCTTCT GCCATTTACA GCGTGGAGAC GATCAATGAT 
GGAAACTTTC ACATTGTGGA ACTACTTGCC CTGGATCAGA GCCTGTCCCT CTCCGTGGAT 
GGAGGGAGCC CCAAAATCAT CACCAACTTG TCAAAGCAGT CCACTCTGAA TTTTGACTCT 
CCACTCTATG TAGGAGGCAT GCCCGGGAGG AACAACGTGG CCGCGGCCCT GCGCCAGGCC 
CCGGGGCACA ACGGCACCAG CTTCCACGGC TGCATCCGGA ACCTGTATAT CAACAGCGAG 
CTCCAGGACT TCCGCCAGGT GCCCATGCAG ACCGGCATCC TGCCCGGCTG CGAGCCGTGC 
CACAGGAAGG TGTGTGCCCA CGGCGCGTGC CAGCCCAGCA GCCAGTCGGG CTTCACCTGC 
GAGTGCGAGG AGGGCTGGAC GGGGCCCCTG TGTGACCAGA GGACCAACGA CCCCTGTCTC 
GGGAACAAAT GTGTGCACGG CACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGTAAG 
TGTCTGGAGG GCCACGGGGG CGTCCTCTGC GACGAAGAGG AGGACCTGTT CAACCCCTGC 
CAGGCCATCA GGTGCAAGCA CGGGAAATGC AGGCTCTCGG GCCTGGGCCA GCCCTACTGC 
GAATGCAGCA GCGGGTACAC GGGGGATAGC TGCGACCGAG AAGTGTCCTG TCGGGGCGAG 
CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG 
GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG 
AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG 
GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272985.1
CDS	16..4257
Translation	MALFSSALHACDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_022417277.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417277.1

ORF Insert Sequence:

ATGGCTCTGT TTTCATCAGC TCTGCACGCG TGTGATCTCA GTGAAAACCA AATTCAGGCA 
ATTCCAAGGA AGGCTTTCCG TGGGGCAGTT GACATTAAAA ATTTGCAACT GGATTACAAC 
CAGATCAGCT GTATTGAAGA TGGGGCATTT AGAGCTCTGC GGGACCTGGA AGTGCTCACT 
CTCAACAATA ACAACATTAC TAGACTTTCT GTGGCAAGTT TCAACCATAT GCCTAAACTT 
AGGACTTTTC GGCTGCATTC AAACAATCTG TATTGCGACT GCCACCTGGC CTGGCTTTCT 
GACTGGCTGC GCCAAAGGCC CCGGGTTGGT CTCTACACTC AGTGTATGGG CCCATCCCAC 
CTGAGGGGTC ATAACGTAGC CGAGGTTCAA AAACGCGAAT TTGTCTGCAG TGATGAGGAA 
GAAGGCCACC AGTCCTTCAT GGCTCCTTCT TGCAGCGTCC TGCATTGTCC AGCCGCTTGT 
ACCTGTAGCA ACAATATCGT AGACTGTCGT GGGAAAGGTC TCACTGAGAT CCCCACGAAC 
CTGCCAGAGA CCATCACAGA AATACGTTTG GAACAGAACT CAATCAAGGT CATCCCTCCT 
GGAGCTTTCT CACCATATAA AAAGCTTAGA AGAATTGACC TGAGCAATAA TCAGATCTCT 
GAACTAGCAC CGGACGCTTT CCAAGGACTA CGCTCTCTGA ATTCACTTGT CCTCTATGGA 
AATAAAATCA CGGAACTCCC AAAAAGTTTA TTTGAAGGAC TGTTTTCCTT ACAGCTGCTA 
TTATTGAATG CCAACAAGAT AAACTGCCTT CGGGTAGATG CTTTTCAGGA TCTGCACAAC 
CTGAATCTTC TCTCCCTGTA CGACAACAAG CTGCAGACCA TCGCCAAGGG GACCTTCTCA 
CCTCTCCGGG CCATTCAGAC CATGCACCTG GCCCAGAACC CCTTTATTTG TGACTGCCAT 
CTCAAGTGGC TGGCGGACTA TCTCCACACC AACCCCATCG AGACCAGTGG TGCCCGGTGC 
ACCAGCCCCC GGCGCCTGGC AAACAAAAGA ATCGGACAGA TCAAAAGCAA GAAATTCCGT 
TGTTCAGCTA AAGAACAGTA TTTCATTCCA GGTACAGAAG ATTATCGATC AAAATTAAGC 
GGGGACTGCT TTGCAGATCT GGCTTGCCCT GAAAAGTGCC GCTGTGAAGG AACCACAGTA 
GATTGCTCCA ATCAAAAACT CACCAAAATC CCAGACCACA TCCCCCAGTA CACTGCAGAG 
CTGCGTCTCA ATAATAATGA ATTCACAGTG CTGGAAGCTA CAGGAATCTT CAAGAAACTT 
CCGCAGTTAC GTAAAATAAA CTTCAGCAAC AACAAGATCA CAGACATTGA AGAAGGAGCA 
TTTGAAGGAG CAGCTGGTGT AAACGAAATC CTTCTCACGA GTAACCGTTT GGAAAATGTT 
CAGCATAAGA TGTTCAAGGG ATTGGAAAGC CTGAAAACGT TGATGTTGCG AAGCAATCGC 
ATAAGCTGCG TTGGCAACGA TAGCTTCATA GGCCTGAGCT CTGTGCGGTT GCTTTCGCTG 
TACGATAATC AGATCGCCAC CATCGCGCCG GGGGCGTTCG ACACCCTGCA CTCGTTGTCC 
ACCCTAAACC TGTTGGCCAA CCCTTTTAAC TGCAACTGCT ACCTGGCTTG GCTGGGCGAG 
TGGCTCAGGA AGAAAAGAAT TGTAACCGGA AATCCTCGCT GTCAAAAACC ATACTTCCTC 
AAAGAAATCC CCATCCAGGA CGTCGCCATT CAAGACTTCA CGTGTGACGA CGGAAATGAC 
GACAGTAGCT GTTCTCCACT CTCGCGCTGT CCCACGGAAT GCACGTGCTT GGATACAGTT 
GTCCGATGTA GCAACAAGGG CCTGAAGGTC TTGCCCAAAG GTATTCCCAG AGACGTCACT 
GAACTGTATC TGGATGGGAA CCACTTTACC TTGGTTCCCA AGGAGCTCTA TAACTACAAA 
CATCTAACGC TTATAGACCT GAGCAACAAC CGCATAAGCA CTCTTTCTAA TCAGAGCTTC 
AGCAACATGA CCCAGCTCCT CACCCTAATT CTCAGTTACA ACCGTTTGAG ATGTATTCCT 
CCTCGAACCT TCGATGGACT CAAGTCTCTC CGATTACTTT CATTACATGG AAATGACATT 
TCTGTTGTGC CTGAAGGTGC TTTCAGTGAT CTCTCTGCAT TATCACACCT AGCAATCGGA 
GCCAACCCCC TTTACTGTGA TTGCAACATG CAGTGGTTAT CGGACTGGGT AAAGTCGGAA 
TACAAAGAAC CCGGGATTGC TCGCTGTGCC GGCCCCGGAG AAATGGCAGA TAAATTATTA 
CTCACGACTC CCTCCAAAAA ATTTACATGT CAAGGTCCTG TGGATATCAA TATTCTAGCT 
AAATGTAATC CCTGCTTATC AAACCCATGT AAGAATGATG GCACCTGTAA CAATGATCCA 
GTCGACTTTT ATCGCTGTAC CTGTCCGTAT GGTTTCAAGG GGCAGGACTG TGATGTCCCA 
ATCCACGCAT GCATCAGTAA CCCGTGTACA CATGGAGGAA CTTGCCACTT AAAGGAGGGA 
GAAAAAGATG GATTCTGGTG TATTTGTGCC GATGGATTTG AAGGAGAAAA TTGTGAAGTC 
AATGTTGATG ACTGTGAAGA TAATGACTGT GAAAATAACT CTACGTGTGT CGATGGAATT 
AATAACTACA CATGCCTTTG TCCGCCTGAG TACACAGGCG AGTTGTGTGA GGAGAAGCTG 
GACTTCTGCG CTCAGGACCT GAACCCCTGC CAGCACGACT CCAAGTGCAT CCTGATGCCC 
AAAGGATTCA AATGCGACTG CACGCCGGGG TACGTGGGCG AGCACTGCGA CATCGACTTC 
GACGACTGCC AGGATCACAA GTGTAAAAAC GGAGCGCACT GCACGGACGC GGTGAACGGC 
TACACGTGCA CCTGCCCCGA AGGCTACAGC GGCTTGTTCT GTGAATTCTC CCCGCCCATG 
GTCCTCCCAC GCACCAGCCC CTGTGACAAC TTCGACTGTC AGAACGGGGC GCAGTGCATC 
GTCAGGGCGG GCGAGCCAAT CTGCCAGTGT CTGCCCGGCT ACCAGGGGGA CAAGTGTGAG 
AAGTTGGTCA GCGTGAACTT CGTGAACAAA GAGTCGTATC TTCAAATTCC TTCAGCCAAG 
GTCCGGCCCC AAACGAACAT CACCCTGCAG ATTGCCACCG ACGAAGACAG CGGGATCCTC 
CTGTACAAGG GCGACAAGGA CCACATTGCC GTGGAGCTGT ATCGGGGACG GGTGCGCGCC 
AGCTACGACA CCGGCTCGCA CCCCGCTTCT GCCATTTACA GCGTGGAGAC GATCAATGAT 
GGAAACTTTC ACATTGTGGA ACTACTTGCC CTGGATCAGA GCCTGTCCCT CTCCGTGGAT 
GGAGGGAGCC CCAAAATCAT CACCAACTTG TCAAAGCAGT CCACTCTGAA TTTTGACTCT 
CCACTCTATG TAGGAGGCAT GCCCGGGAGG AACAACGTGG CCGCGGCCCT GCGCCAGGCC 
CCGGGGCACA ACGGCACCAG CTTCCACGGC TGCATCCGGA ACCTGTATAT CAACAGCGAG 
CTCCAGGACT TCCGCCAGGT GCCCATGCAG ACCGGCATCC TGCCCGGCTG CGAGCCGTGC 
CACAGGAAGG TGTGTGCCCA CGGCGCGTGC CAGCCCAGCA GCCAGTCGGG CTTCACCTGC 
GAGTGCGAGG AGGGCTGGAC GGGGCCCCTG TGTGACCAGA GGACCAACGA CCCCTGTCTC 
GGGAACAAAT GTGTGCACGG CACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGTAAG 
TGTCTGGAGG GCCACGGGGG CGTCCTCTGC GACGAAGAGG AGGACCTGTT CAACCCCTGC 
CAGGCCATCA GGTGCAAGCA CGGGAAATGC AGGCTCTCGG GCCTGGGCCA GCCCTACTGC 
GAATGCAGCA GCGGGTACAC GGGGGATAGC TGCGACCGAG AAGTGTCCTG TCGGGGCGAG 
CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG 
GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG 
AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG 
GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_022417276.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272984.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe287415
Clone ID Related Accession (Same CDS sequence)	XM_022417276.1
Accession Version	XM_022417276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT 
AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC 
TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC 
TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGTGTCCTG TCGGGGCGAG 
CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG 
GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG 
AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG 
GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272984.1
CDS	213..4814
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDRVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_022417276.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417276.1

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT 
AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC 
TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC 
TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGTGTCCTG TCGGGGCGAG 
CGCGTCCGGG ACTACTACCC AAAGCAGCAG GGCTACGCGG CCTGCCAGAC CACCAAGAAG 
GTGTCGCGGC TGGAGTGCAG GGGCGGCTGC GCGGCCGGGC AGTGCTGCGG GCCGCTGCGG 
AGCAAGCGGC GGAAATACTC CTTCGAGTGC ACGGACGGCT CGTCGTTCGT GGACGAGGTG 
GAGAAGGTGG TCAAGTGCGG CTGCAGCAGG TGCGCCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112671.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968146.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

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CloneID	OCe35885
Clone ID Related Accession (Same CDS sequence)	XM_014112671.1 , XM_014112671.2
Accession Version	XM_014112671.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	slit homolog 2 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT 
AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC 
TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC 
TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC 
GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG 
AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG 
CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG 
GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968146.1
CDS	86..4690
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112671.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112671.1

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT 
AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC 
TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC 
TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC 
GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG 
AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG 
CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG 
GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112671.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968146.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

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CloneID	OCe35885
Clone ID Related Accession (Same CDS sequence)	XM_014112671.1 , XM_014112671.2
Accession Version	XM_014112671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT 
AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC 
TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC 
TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC 
GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG 
AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG 
CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG 
GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968146.1
CDS	212..4816
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112671.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112671.2

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA AATGTGTGCA CGGCACCTGC TTGCCCATCA ACGCCTTCTC CTACAGCTGT 
AAGTGTCTGG AGGGCCACGG GGGCGTCCTC TGCGACGAAG AGGAGGACCT GTTCAACCCC 
TGCCAGGCCA TCAGGTGCAA GCACGGGAAA TGCAGGCTCT CGGGCCTGGG CCAGCCCTAC 
TGCGAATGCA GCAGCGGGTA CACGGGGGAT AGCTGCGACC GAGAAGTGTC CTGTCGGGGC 
GAGCGCGTCC GGGACTACTA CCCAAAGCAG CAGGGCTACG CGGCCTGCCA GACCACCAAG 
AAGGTGTCGC GGCTGGAGTG CAGGGGCGGC TGCGCGGCCG GGCAGTGCTG CGGGCCGCTG 
CGGAGCAAGC GGCGGAAATA CTCCTTCGAG TGCACGGACG GCTCGTCGTT CGTGGACGAG 
GTGGAGAAGG TGGTCAAGTG CGGCTGCAGC AGGTGCGCCG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112672.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968147.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

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CloneID	OCe35886
Clone ID Related Accession (Same CDS sequence)	XM_014112672.2 , XM_014112672.1
Accession Version	XM_014112672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC 
TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA 
CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA 
GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC 
GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT 
CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC 
CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG 
AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC 
CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC 
TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT 
AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT 
CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG 
CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC 
CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA 
CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT 
TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA 
TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC 
GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC 
AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC 
CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC 
ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA 
CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG 
GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC 
AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC 
CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG 
GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC 
ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT 
CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC 
CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC 
AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC 
TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG 
GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG 
GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA 
TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG 
GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC 
AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC 
AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG 
GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG 
CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG 
CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG 
GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968147.1
CDS	212..4804
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112672.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112672.2

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC 
TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA 
CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA 
GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC 
GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT 
CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC 
CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG 
AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC 
CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC 
TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT 
AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT 
CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG 
CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC 
CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA 
CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT 
TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA 
TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC 
GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC 
AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC 
CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC 
ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA 
CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG 
GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC 
AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC 
CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG 
GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC 
ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT 
CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC 
CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC 
AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC 
TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG 
GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG 
GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA 
TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG 
GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC 
AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC 
AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG 
GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG 
CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG 
CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG 
GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112672.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968147.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe35886
Clone ID Related Accession (Same CDS sequence)	XM_014112672.2 , XM_014112672.1
Accession Version	XM_014112672.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	slit homolog 2 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC 
TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA 
CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA 
GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC 
GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT 
CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC 
CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG 
AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC 
CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC 
TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT 
AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT 
CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG 
CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC 
CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA 
CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT 
TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA 
TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC 
GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC 
AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC 
CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC 
ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA 
CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG 
GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC 
AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC 
CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG 
GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC 
ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT 
CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC 
CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC 
AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC 
TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG 
GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG 
GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA 
TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG 
GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC 
AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC 
AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG 
GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG 
CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG 
CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG 
GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968147.1
CDS	86..4678
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112672.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112672.1

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG CGGGGACTGC 
TTTGCAGATC TGGCTTGCCC TGAAAAGTGC CGCTGTGAAG GAACCACAGT AGATTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGACCAC ATCCCCCAGT ACACTGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GCTGGAAGCT ACAGGAATCT TCAAGAAACT TCCGCAGTTA 
CGTAAAATAA ACTTCAGCAA CAACAAGATC ACAGACATTG AAGAAGGAGC ATTTGAAGGA 
GCAGCTGGTG TAAACGAAAT CCTTCTCACG AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GATTGGAAAG CCTGAAAACG TTGATGTTGC GAAGCAATCG CATAAGCTGC 
GTTGGCAACG ATAGCTTCAT AGGCCTGAGC TCTGTGCGGT TGCTTTCGCT GTACGATAAT 
CAGATCGCCA CCATCGCGCC GGGGGCGTTC GACACCCTGC ACTCGTTGTC CACCCTAAAC 
CTGTTGGCCA ACCCTTTTAA CTGCAACTGC TACCTGGCTT GGCTGGGCGA GTGGCTCAGG 
AAGAAAAGAA TTGTAACCGG AAATCCTCGC TGTCAAAAAC CATACTTCCT CAAAGAAATC 
CCCATCCAGG ACGTCGCCAT TCAAGACTTC ACGTGTGACG ACGGAAATGA CGACAGTAGC 
TGTTCTCCAC TCTCGCGCTG TCCCACGGAA TGCACGTGCT TGGATACAGT TGTCCGATGT 
AGCAACAAGG GCCTGAAGGT CTTGCCCAAA GGTATTCCCA GAGACGTCAC TGAACTGTAT 
CTGGATGGGA ACCACTTTAC CTTGGTTCCC AAGGAGCTCT ATAACTACAA ACATCTAACG 
CTTATAGACC TGAGCAACAA CCGCATAAGC ACTCTTTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACCCTAAT TCTCAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TCAAGTCTCT CCGATTACTT TCATTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAGTGA TCTCTCTGCA TTATCACACC TAGCAATCGG AGCCAACCCC 
CTTTACTGTG ATTGCAACAT GCAGTGGTTA TCGGACTGGG TAAAGTCGGA ATACAAAGAA 
CCCGGGATTG CTCGCTGTGC CGGCCCCGGA GAAATGGCAG ATAAATTATT ACTCACGACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATATCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAACCCATG TAAGAATGAT GGCACCTGTA ACAATGATCC AGTCGACTTT 
TATCGCTGTA CCTGTCCGTA TGGTTTCAAG GGGCAGGACT GTGATGTCCC AATCCACGCA 
TGCATCAGTA ACCCGTGTAC ACATGGAGGA ACTTGCCACT TAAAGGAGGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC CGATGGATTT GAAGGAGAAA ATTGTGAAGT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACGTGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GTCCGCCTGA GTACACAGGC GAGTTGTGTG AGGAGAAGCT GGACTTCTGC 
GCTCAGGACC TGAACCCCTG CCAGCACGAC TCCAAGTGCA TCCTGATGCC CAAAGGATTC 
AAATGCGACT GCACGCCGGG GTACGTGGGC GAGCACTGCG ACATCGACTT CGACGACTGC 
CAGGATCACA AGTGTAAAAA CGGAGCGCAC TGCACGGACG CGGTGAACGG CTACACGTGC 
ACCTGCCCCG AAGGCTACAG CGGCTTGTTC TGTGAATTCT CCCCGCCCAT GGTCCTCCCA 
CGCACCAGCC CCTGTGACAA CTTCGACTGT CAGAACGGGG CGCAGTGCAT CGTCAGGGCG 
GGCGAGCCAA TCTGCCAGTG TCTGCCCGGC TACCAGGGGG ACAAGTGTGA GAAGTTGGTC 
AGCGTGAACT TCGTGAACAA AGAGTCGTAT CTTCAAATTC CTTCAGCCAA GGTCCGGCCC 
CAAACGAACA TCACCCTGCA GATTGCCACC GACGAAGACA GCGGGATCCT CCTGTACAAG 
GGCGACAAGG ACCACATTGC CGTGGAGCTG TATCGGGGAC GGGTGCGCGC CAGCTACGAC 
ACCGGCTCGC ACCCCGCTTC TGCCATTTAC AGCGTGGAGA CGATCAATGA TGGAAACTTT 
CACATTGTGG AACTACTTGC CCTGGATCAG AGCCTGTCCC TCTCCGTGGA TGGAGGGAGC 
CCCAAAATCA TCACCAACTT GTCAAAGCAG TCCACTCTGA ATTTTGACTC TCCACTCTAT 
GTAGGAGGCA TGCCCGGGAG GAACAACGTG GCCGCGGCCC TGCGCCAGGC CCCGGGGCAC 
AACGGCACCA GCTTCCACGG CTGCATCCGG AACCTGTATA TCAACAGCGA GCTCCAGGAC 
TTCCGCCAGG TGCCCATGCA GACCGGCATC CTGCCCGGCT GCGAGCCGTG CCACAGGAAG 
GTGTGTGCCC ACGGCGCGTG CCAGCCCAGC AGCCAGTCGG GCTTCACCTG CGAGTGCGAG 
GAGGGCTGGA CGGGGCCCCT GTGTGACCAG AGGACCAACG ACCCCTGTCT CGGGAACAAA 
TGTGTGCACG GCACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGTAA GTGTCTGGAG 
GGCCACGGGG GCGTCCTCTG CGACGAAGAG GAGGACCTGT TCAACCCCTG CCAGGCCATC 
AGGTGCAAGC ACGGGAAATG CAGGCTCTCG GGCCTGGGCC AGCCCTACTG CGAATGCAGC 
AGCGGGTACA CGGGGGATAG CTGCGACCGA GAAGTGTCCT GTCGGGGCGA GCGCGTCCGG 
GACTACTACC CAAAGCAGCA GGGCTACGCG GCCTGCCAGA CCACCAAGAA GGTGTCGCGG 
CTGGAGTGCA GGGGCGGCTG CGCGGCCGGG CAGTGCTGCG GGCCGCTGCG GAGCAAGCGG 
CGGAAATACT CCTTCGAGTG CACGGACGGC TCGTCGTTCG TGGACGAGGT GGAGAAGGTG 
GTCAAGTGCG GCTGCAGCAG GTGCGCCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112673.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968148.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35887
Clone ID Related Accession (Same CDS sequence)	XM_014112673.1 , XM_014112673.2
Accession Version	XM_014112673.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	slit homolog 2 protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG 
GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC 
ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC 
TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA 
AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT 
AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC 
ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC 
CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT 
GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC 
GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC 
TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC 
CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC 
CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG 
AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC 
AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT 
TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT 
CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA 
AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC 
TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC 
CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT 
ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT 
AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT 
CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG 
AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC 
ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG 
TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA 
GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC 
TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC 
TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC 
GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC 
ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC 
CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC 
CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA 
CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC 
ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC 
TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG 
CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC 
GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG 
GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC 
ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC 
GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC 
GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG 
GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA 
AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG 
GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC 
TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC 
TGCAGCAGGT GCGCCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968148.1
CDS	86..4666
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112673.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112673.1

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG 
GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC 
ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC 
TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA 
AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT 
AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC 
ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC 
CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT 
GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC 
GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC 
TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC 
CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC 
CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG 
AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC 
AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT 
TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT 
CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA 
AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC 
TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC 
CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT 
ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT 
AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT 
CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG 
AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC 
ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG 
TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA 
GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC 
TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC 
TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC 
GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC 
ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC 
CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC 
CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA 
CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC 
ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC 
TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG 
CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC 
GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG 
GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC 
ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC 
GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC 
GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG 
GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA 
AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG 
GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC 
TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC 
TGCAGCAGGT GCGCCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112673.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968148.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

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CloneID	OCe35887
Clone ID Related Accession (Same CDS sequence)	XM_014112673.1 , XM_014112673.2
Accession Version	XM_014112673.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG 
GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC 
ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC 
TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA 
AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT 
AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC 
ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC 
CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT 
GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC 
GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC 
TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC 
CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC 
CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG 
AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC 
AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT 
TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT 
CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA 
AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC 
TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC 
CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT 
ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT 
AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT 
CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG 
AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC 
ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG 
TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA 
GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC 
TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC 
TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC 
GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC 
ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC 
CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC 
CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA 
CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC 
ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC 
TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG 
CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC 
GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG 
GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC 
ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC 
GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC 
GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG 
GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA 
AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG 
GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC 
TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC 
TGCAGCAGGT GCGCCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968148.1
CDS	212..4792
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112673.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112673.2

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGCG GGGACTGCTT TGCAGATCTG 
GCTTGCCCTG AAAAGTGCCG CTGTGAAGGA ACCACAGTAG ATTGCTCCAA TCAAAAACTC 
ACCAAAATCC CAGACCACAT CCCCCAGTAC ACTGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGC TGGAAGCTAC AGGAATCTTC AAGAAACTTC CGCAGTTACG TAAAATAAAC 
TTCAGCAACA ACAAGATCAC AGACATTGAA GAAGGAGCAT TTGAAGGAGC AGCTGGTGTA 
AACGAAATCC TTCTCACGAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGA 
TTGGAAAGCC TGAAAACGTT GATGTTGCGA AGCAATCGCA TAAGCTGCGT TGGCAACGAT 
AGCTTCATAG GCCTGAGCTC TGTGCGGTTG CTTTCGCTGT ACGATAATCA GATCGCCACC 
ATCGCGCCGG GGGCGTTCGA CACCCTGCAC TCGTTGTCCA CCCTAAACCT GTTGGCCAAC 
CCTTTTAACT GCAACTGCTA CCTGGCTTGG CTGGGCGAGT GGCTCAGGAA GAAAAGAATT 
GTAACCGGAA ATCCTCGCTG TCAAAAACCA TACTTCCTCA AAGAAATCCC CATCCAGGAC 
GTCGCCATTC AAGACTTCAC GTGTGACGAC GGAAATGACG ACAGTAGCTG TTCTCCACTC 
TCGCGCTGTC CCACGGAATG CACGTGCTTG GATACAGTTG TCCGATGTAG CAACAAGGGC 
CTGAAGGTCT TGCCCAAAGG TATTCCCAGA GACGTCACTG AACTGTATCT GGATGGGAAC 
CACTTTACCT TGGTTCCCAA GGAGCTCTAT AACTACAAAC ATCTAACGCT TATAGACCTG 
AGCAACAACC GCATAAGCAC TCTTTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACCCTAATTC TCAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTC 
AAGTCTCTCC GATTACTTTC ATTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAGTGATC TCTCTGCATT ATCACACCTA GCAATCGGAG CCAACCCCCT TTACTGTGAT 
TGCAACATGC AGTGGTTATC GGACTGGGTA AAGTCGGAAT ACAAAGAACC CGGGATTGCT 
CGCTGTGCCG GCCCCGGAGA AATGGCAGAT AAATTATTAC TCACGACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCTGT GGATATCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA 
AACCCATGTA AGAATGATGG CACCTGTAAC AATGATCCAG TCGACTTTTA TCGCTGTACC 
TGTCCGTATG GTTTCAAGGG GCAGGACTGT GATGTCCCAA TCCACGCATG CATCAGTAAC 
CCGTGTACAC ATGGAGGAAC TTGCCACTTA AAGGAGGGAG AAAAAGATGG ATTCTGGTGT 
ATTTGTGCCG ATGGATTTGA AGGAGAAAAT TGTGAAGTCA ATGTTGATGA CTGTGAAGAT 
AATGACTGTG AAAATAACTC TACGTGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGT 
CCGCCTGAGT ACACAGGCGA GTTGTGTGAG GAGAAGCTGG ACTTCTGCGC TCAGGACCTG 
AACCCCTGCC AGCACGACTC CAAGTGCATC CTGATGCCCA AAGGATTCAA ATGCGACTGC 
ACGCCGGGGT ACGTGGGCGA GCACTGCGAC ATCGACTTCG ACGACTGCCA GGATCACAAG 
TGTAAAAACG GAGCGCACTG CACGGACGCG GTGAACGGCT ACACGTGCAC CTGCCCCGAA 
GGCTACAGCG GCTTGTTCTG TGAATTCTCC CCGCCCATGG TCCTCCCACG CACCAGCCCC 
TGTGACAACT TCGACTGTCA GAACGGGGCG CAGTGCATCG TCAGGGCGGG CGAGCCAATC 
TGCCAGTGTC TGCCCGGCTA CCAGGGGGAC AAGTGTGAGA AGTTGGTCAG CGTGAACTTC 
GTGAACAAAG AGTCGTATCT TCAAATTCCT TCAGCCAAGG TCCGGCCCCA AACGAACATC 
ACCCTGCAGA TTGCCACCGA CGAAGACAGC GGGATCCTCC TGTACAAGGG CGACAAGGAC 
CACATTGCCG TGGAGCTGTA TCGGGGACGG GTGCGCGCCA GCTACGACAC CGGCTCGCAC 
CCCGCTTCTG CCATTTACAG CGTGGAGACG ATCAATGATG GAAACTTTCA CATTGTGGAA 
CTACTTGCCC TGGATCAGAG CCTGTCCCTC TCCGTGGATG GAGGGAGCCC CAAAATCATC 
ACCAACTTGT CAAAGCAGTC CACTCTGAAT TTTGACTCTC CACTCTATGT AGGAGGCATG 
CCCGGGAGGA ACAACGTGGC CGCGGCCCTG CGCCAGGCCC CGGGGCACAA CGGCACCAGC 
TTCCACGGCT GCATCCGGAA CCTGTATATC AACAGCGAGC TCCAGGACTT CCGCCAGGTG 
CCCATGCAGA CCGGCATCCT GCCCGGCTGC GAGCCGTGCC ACAGGAAGGT GTGTGCCCAC 
GGCGCGTGCC AGCCCAGCAG CCAGTCGGGC TTCACCTGCG AGTGCGAGGA GGGCTGGACG 
GGGCCCCTGT GTGACCAGAG GACCAACGAC CCCTGTCTCG GGAACAAATG TGTGCACGGC 
ACCTGCTTGC CCATCAACGC CTTCTCCTAC AGCTGTAAGT GTCTGGAGGG CCACGGGGGC 
GTCCTCTGCG ACGAAGAGGA GGACCTGTTC AACCCCTGCC AGGCCATCAG GTGCAAGCAC 
GGGAAATGCA GGCTCTCGGG CCTGGGCCAG CCCTACTGCG AATGCAGCAG CGGGTACACG 
GGGGATAGCT GCGACCGAGA AGTGTCCTGT CGGGGCGAGC GCGTCCGGGA CTACTACCCA 
AAGCAGCAGG GCTACGCGGC CTGCCAGACC ACCAAGAAGG TGTCGCGGCT GGAGTGCAGG 
GGCGGCTGCG CGGCCGGGCA GTGCTGCGGG CCGCTGCGGA GCAAGCGGCG GAAATACTCC 
TTCGAGTGCA CGGACGGCTC GTCGTTCGTG GACGAGGTGG AGAAGGTGGT CAAGTGCGGC 
TGCAGCAGGT GCGCCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112674.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968149.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

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CloneID	OCe35888
Clone ID Related Accession (Same CDS sequence)	XM_014112674.1 , XM_014112674.2
Accession Version	XM_014112674.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	slit homolog 2 protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA 
AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG 
GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC 
AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT 
CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG 
AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC 
CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG 
GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC 
AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT 
CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA 
GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC 
ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG 
CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG 
GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC 
ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA 
TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC 
TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG 
TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC 
CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG 
AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT 
GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC 
ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG 
CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC 
GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA 
GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC 
TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC 
GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG 
CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG 
TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT 
GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG 
GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC 
ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC 
AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC 
ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC 
GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG 
CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT 
GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC 
ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC 
GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG 
CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC 
GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC 
TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG 
GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG 
GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC 
GCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968149.1
CDS	86..4654
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112674.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112674.1

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA 
AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG 
GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC 
AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT 
CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG 
AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC 
CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG 
GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC 
AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT 
CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA 
GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC 
ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG 
CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG 
GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC 
ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA 
TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC 
TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG 
TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC 
CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG 
AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT 
GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC 
ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG 
CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC 
GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA 
GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC 
TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC 
GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG 
CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG 
TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT 
GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG 
GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC 
ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC 
AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC 
ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC 
GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG 
CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT 
GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC 
ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC 
GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG 
CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC 
GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC 
TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG 
GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG 
GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC 
GCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112674.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968149.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe35888
Clone ID Related Accession (Same CDS sequence)	XM_014112674.1 , XM_014112674.2
Accession Version	XM_014112674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA 
AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG 
GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC 
AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT 
CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG 
AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC 
CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG 
GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC 
AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT 
CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA 
GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC 
ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG 
CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG 
GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC 
ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA 
TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC 
TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG 
TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC 
CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG 
AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT 
GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC 
ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG 
CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC 
GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA 
GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC 
TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC 
GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG 
CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG 
TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT 
GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG 
GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC 
ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC 
AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC 
ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC 
GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG 
CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT 
GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC 
ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC 
GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG 
CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC 
GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC 
TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG 
GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG 
GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC 
GCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968149.1
CDS	212..4780
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112674.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112674.2

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGGCCAC 
CAGTCCTTCA TGGCTCCTTC TTGCAGCGTC CTGCATTGTC CAGCCGCTTG TACCTGTAGC 
AACAATATCG TAGACTGTCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA CCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATTGAC CTGAGCAATA ATCAGATCTC TGAACTAGCA 
CCGGACGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACGGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTGCT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAATCTT 
CTCTCCCTGT ACGACAACAA GCTGCAGACC ATCGCCAAGG GGACCTTCTC ACCTCTCCGG 
GCCATTCAGA CCATGCACCT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGACT ATCTCCACAC CAACCCCATC GAGACCAGTG GTGCCCGGTG CACCAGCCCC 
CGGCGCCTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGCGGG GACTGCTTTG CAGATCTGGC TTGCCCTGAA 
AAGTGCCGCT GTGAAGGAAC CACAGTAGAT TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GACCACATCC CCCAGTACAC TGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGCTG 
GAAGCTACAG GAATCTTCAA GAAACTTCCG CAGTTACGTA AAATAAACTT CAGCAACAAC 
AAGATCACAG ACATTGAAGA AGGAGCATTT GAAGGAGCAG CTGGTGTAAA CGAAATCCTT 
CTCACGAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGATT GGAAAGCCTG 
AAAACGTTGA TGTTGCGAAG CAATCGCATA AGCTGCGTTG GCAACGATAG CTTCATAGGC 
CTGAGCTCTG TGCGGTTGCT TTCGCTGTAC GATAATCAGA TCGCCACCAT CGCGCCGGGG 
GCGTTCGACA CCCTGCACTC GTTGTCCACC CTAAACCTGT TGGCCAACCC TTTTAACTGC 
AACTGCTACC TGGCTTGGCT GGGCGAGTGG CTCAGGAAGA AAAGAATTGT AACCGGAAAT 
CCTCGCTGTC AAAAACCATA CTTCCTCAAA GAAATCCCCA TCCAGGACGT CGCCATTCAA 
GACTTCACGT GTGACGACGG AAATGACGAC AGTAGCTGTT CTCCACTCTC GCGCTGTCCC 
ACGGAATGCA CGTGCTTGGA TACAGTTGTC CGATGTAGCA ACAAGGGCCT GAAGGTCTTG 
CCCAAAGGTA TTCCCAGAGA CGTCACTGAA CTGTATCTGG ATGGGAACCA CTTTACCTTG 
GTTCCCAAGG AGCTCTATAA CTACAAACAT CTAACGCTTA TAGACCTGAG CAACAACCGC 
ATAAGCACTC TTTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC CCTAATTCTC 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTCAA GTCTCTCCGA 
TTACTTTCAT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCTTT CAGTGATCTC 
TCTGCATTAT CACACCTAGC AATCGGAGCC AACCCCCTTT ACTGTGATTG CAACATGCAG 
TGGTTATCGG ACTGGGTAAA GTCGGAATAC AAAGAACCCG GGATTGCTCG CTGTGCCGGC 
CCCGGAGAAA TGGCAGATAA ATTATTACTC ACGACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATATCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA CCCATGTAAG 
AATGATGGCA CCTGTAACAA TGATCCAGTC GACTTTTATC GCTGTACCTG TCCGTATGGT 
TTCAAGGGGC AGGACTGTGA TGTCCCAATC CACGCATGCA TCAGTAACCC GTGTACACAT 
GGAGGAACTT GCCACTTAAA GGAGGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCCGAT 
GGATTTGAAG GAGAAAATTG TGAAGTCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CGTGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGTCC GCCTGAGTAC 
ACAGGCGAGT TGTGTGAGGA GAAGCTGGAC TTCTGCGCTC AGGACCTGAA CCCCTGCCAG 
CACGACTCCA AGTGCATCCT GATGCCCAAA GGATTCAAAT GCGACTGCAC GCCGGGGTAC 
GTGGGCGAGC ACTGCGACAT CGACTTCGAC GACTGCCAGG ATCACAAGTG TAAAAACGGA 
GCGCACTGCA CGGACGCGGT GAACGGCTAC ACGTGCACCT GCCCCGAAGG CTACAGCGGC 
TTGTTCTGTG AATTCTCCCC GCCCATGGTC CTCCCACGCA CCAGCCCCTG TGACAACTTC 
GACTGTCAGA ACGGGGCGCA GTGCATCGTC AGGGCGGGCG AGCCAATCTG CCAGTGTCTG 
CCCGGCTACC AGGGGGACAA GTGTGAGAAG TTGGTCAGCG TGAACTTCGT GAACAAAGAG 
TCGTATCTTC AAATTCCTTC AGCCAAGGTC CGGCCCCAAA CGAACATCAC CCTGCAGATT 
GCCACCGACG AAGACAGCGG GATCCTCCTG TACAAGGGCG ACAAGGACCA CATTGCCGTG 
GAGCTGTATC GGGGACGGGT GCGCGCCAGC TACGACACCG GCTCGCACCC CGCTTCTGCC 
ATTTACAGCG TGGAGACGAT CAATGATGGA AACTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAGAGCC TGTCCCTCTC CGTGGATGGA GGGAGCCCCA AAATCATCAC CAACTTGTCA 
AAGCAGTCCA CTCTGAATTT TGACTCTCCA CTCTATGTAG GAGGCATGCC CGGGAGGAAC 
AACGTGGCCG CGGCCCTGCG CCAGGCCCCG GGGCACAACG GCACCAGCTT CCACGGCTGC 
ATCCGGAACC TGTATATCAA CAGCGAGCTC CAGGACTTCC GCCAGGTGCC CATGCAGACC 
GGCATCCTGC CCGGCTGCGA GCCGTGCCAC AGGAAGGTGT GTGCCCACGG CGCGTGCCAG 
CCCAGCAGCC AGTCGGGCTT CACCTGCGAG TGCGAGGAGG GCTGGACGGG GCCCCTGTGT 
GACCAGAGGA CCAACGACCC CTGTCTCGGG AACAAATGTG TGCACGGCAC CTGCTTGCCC 
ATCAACGCCT TCTCCTACAG CTGTAAGTGT CTGGAGGGCC ACGGGGGCGT CCTCTGCGAC 
GAAGAGGAGG ACCTGTTCAA CCCCTGCCAG GCCATCAGGT GCAAGCACGG GAAATGCAGG 
CTCTCGGGCC TGGGCCAGCC CTACTGCGAA TGCAGCAGCG GGTACACGGG GGATAGCTGC 
GACCGAGAAG TGTCCTGTCG GGGCGAGCGC GTCCGGGACT ACTACCCAAA GCAGCAGGGC 
TACGCGGCCT GCCAGACCAC CAAGAAGGTG TCGCGGCTGG AGTGCAGGGG CGGCTGCGCG 
GCCGGGCAGT GCTGCGGGCC GCTGCGGAGC AAGCGGCGGA AATACTCCTT CGAGTGCACG 
GACGGCTCGT CGTTCGTGGA CGAGGTGGAG AAGGTGGTCA AGTGCGGCTG CAGCAGGTGC 
GCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112675.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968150.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe35889
Clone ID Related Accession (Same CDS sequence)	XM_014112675.2 , XM_014112675.1
Accession Version	XM_014112675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	slit homolog 2 protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG 
GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC 
CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA 
GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC 
TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG 
TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG 
TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968150.1
CDS	213..4772
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNNCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112675.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112675.2

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG 
GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC 
CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA 
GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC 
TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG 
TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG 
TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_014112675.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968150.1 Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

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CloneID	OCe35889
Clone ID Related Accession (Same CDS sequence)	XM_014112675.2 , XM_014112675.1
Accession Version	XM_014112675.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	slit homolog 2 protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG 
GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC 
CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA 
GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC 
TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG 
TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG 
TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968150.1
CDS	86..4645
Translation	MRGAGRRALPVSLGLVLLILGEAAPQACPAQCSCSGSTVDCHGLALRSVPRSIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGAAGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQIATIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDSSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNHFTLVPKELYNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFSDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDINILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCTHGGTCHLKEGEKDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILMPKGFKCDCTPGYVGEHCDIDFDDCQDHKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIVRAGEPICQCLPGYQGDKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGRNNVAAALRQAPGHNGTSFHGCIRNLYINSELQDFRQVPMQTGILPGCEPCHRKVCAHGACQPSSQSGFTCECEEGWTGPLCDQRTNDPCLGNNCKCLEGHGGVLCDEEEDLFNPCQAIRCKHGKCRLSGLGQPYCECSSGYTGDSCDREVSCRGERVRDYYPKQQGYAACQTTKKVSRLECRGGCAAGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCSRCAA

Target ORF information:

RefSeq Version	XM_014112675.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112675.1

ORF Insert Sequence:

ATGCGCGGGG CCGGCCGGCG GGCGCTGCCC GTGTCGCTGG GGCTCGTGCT GCTGATCCTG 
GGCGAGGCGG CGCCGCAGGC GTGCCCGGCG CAGTGCTCCT GCTCGGGCAG CACCGTGGAC 
TGTCACGGGC TGGCGCTGCG CAGCGTGCCC AGGAGCATCC CCCGCAACAC CGAGAGGCTG 
GATTTGAATG GCAATAACAT CACACGGATT ACCAAGACAG ATTTCGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTAGCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAGG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTGTAC AGGCTTGATC TCAGTGAAAA CCAAATTCAG 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAATTTGCA ACTGGATTAC 
AACCAGATCA GCTGTATTGA AGATGGGGCA TTTAGAGCTC TGCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGGCTGCA TTCAAACAAT CTGTATTGCG ACTGCCACCT GGCCTGGCTT 
TCTGACTGGC TGCGCCAAAG GCCCCGGGTT GGTCTCTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GTCATAACGT AGCCGAGGTT CAAAAACGCG AATTTGTCTG CAGTGATGAG 
GAAGAAGGCC ACCAGTCCTT CATGGCTCCT TCTTGCAGCG TCCTGCATTG TCCAGCCGCT 
TGTACCTGTA GCAACAATAT CGTAGACTGT CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AACCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATTG ACCTGAGCAA TAATCAGATC 
TCTGAACTAG CACCGGACGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACGGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTG 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAATC TTCTCTCCCT GTACGACAAC AAGCTGCAGA CCATCGCCAA GGGGACCTTC 
TCACCTCTCC GGGCCATTCA GACCATGCAC CTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA CTATCTCCAC ACCAACCCCA TCGAGACCAG TGGTGCCCGG 
TGCACCAGCC CCCGGCGCCT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGCGGGGACT GCTTTGCAGA TCTGGCTTGC CCTGAAAAGT GCCGCTGTGA AGGAACCACA 
GTAGATTGCT CCAATCAAAA ACTCACCAAA ATCCCAGACC ACATCCCCCA GTACACTGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGCTGGAAG CTACAGGAAT CTTCAAGAAA 
CTTCCGCAGT TACGTAAAAT AAACTTCAGC AACAACAAGA TCACAGACAT TGAAGAAGGA 
GCATTTGAAG GAGCAGCTGG TGTAAACGAA ATCCTTCTCA CGAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGATTGGAA AGCCTGAAAA CGTTGATGTT GCGAAGCAAT 
CGCATAAGCT GCGTTGGCAA CGATAGCTTC ATAGGCCTGA GCTCTGTGCG GTTGCTTTCG 
CTGTACGATA ATCAGATCGC CACCATCGCG CCGGGGGCGT TCGACACCCT GCACTCGTTG 
TCCACCCTAA ACCTGTTGGC CAACCCTTTT AACTGCAACT GCTACCTGGC TTGGCTGGGC 
GAGTGGCTCA GGAAGAAAAG AATTGTAACC GGAAATCCTC GCTGTCAAAA ACCATACTTC 
CTCAAAGAAA TCCCCATCCA GGACGTCGCC ATTCAAGACT TCACGTGTGA CGACGGAAAT 
GACGACAGTA GCTGTTCTCC ACTCTCGCGC TGTCCCACGG AATGCACGTG CTTGGATACA 
GTTGTCCGAT GTAGCAACAA GGGCCTGAAG GTCTTGCCCA AAGGTATTCC CAGAGACGTC 
ACTGAACTGT ATCTGGATGG GAACCACTTT ACCTTGGTTC CCAAGGAGCT CTATAACTAC 
AAACATCTAA CGCTTATAGA CCTGAGCAAC AACCGCATAA GCACTCTTTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACCCTA ATTCTCAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTCAAGTCT CTCCGATTAC TTTCATTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAGT GATCTCTCTG CATTATCACA CCTAGCAATC 
GGAGCCAACC CCCTTTACTG TGATTGCAAC ATGCAGTGGT TATCGGACTG GGTAAAGTCG 
GAATACAAAG AACCCGGGAT TGCTCGCTGT GCCGGCCCCG GAGAAATGGC AGATAAATTA 
TTACTCACGA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATAT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAACCCA TGTAAGAATG ATGGCACCTG TAACAATGAT 
CCAGTCGACT TTTATCGCTG TACCTGTCCG TATGGTTTCA AGGGGCAGGA CTGTGATGTC 
CCAATCCACG CATGCATCAG TAACCCGTGT ACACATGGAG GAACTTGCCA CTTAAAGGAG 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCCGATGGAT TTGAAGGAGA AAATTGTGAA 
GTCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACGTG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGTCCGCCT GAGTACACAG GCGAGTTGTG TGAGGAGAAG 
CTGGACTTCT GCGCTCAGGA CCTGAACCCC TGCCAGCACG ACTCCAAGTG CATCCTGATG 
CCCAAAGGAT TCAAATGCGA CTGCACGCCG GGGTACGTGG GCGAGCACTG CGACATCGAC 
TTCGACGACT GCCAGGATCA CAAGTGTAAA AACGGAGCGC ACTGCACGGA CGCGGTGAAC 
GGCTACACGT GCACCTGCCC CGAAGGCTAC AGCGGCTTGT TCTGTGAATT CTCCCCGCCC 
ATGGTCCTCC CACGCACCAG CCCCTGTGAC AACTTCGACT GTCAGAACGG GGCGCAGTGC 
ATCGTCAGGG CGGGCGAGCC AATCTGCCAG TGTCTGCCCG GCTACCAGGG GGACAAGTGT 
GAGAAGTTGG TCAGCGTGAA CTTCGTGAAC AAAGAGTCGT ATCTTCAAAT TCCTTCAGCC 
AAGGTCCGGC CCCAAACGAA CATCACCCTG CAGATTGCCA CCGACGAAGA CAGCGGGATC 
CTCCTGTACA AGGGCGACAA GGACCACATT GCCGTGGAGC TGTATCGGGG ACGGGTGCGC 
GCCAGCTACG ACACCGGCTC GCACCCCGCT TCTGCCATTT ACAGCGTGGA GACGATCAAT 
GATGGAAACT TTCACATTGT GGAACTACTT GCCCTGGATC AGAGCCTGTC CCTCTCCGTG 
GATGGAGGGA GCCCCAAAAT CATCACCAAC TTGTCAAAGC AGTCCACTCT GAATTTTGAC 
TCTCCACTCT ATGTAGGAGG CATGCCCGGG AGGAACAACG TGGCCGCGGC CCTGCGCCAG 
GCCCCGGGGC ACAACGGCAC CAGCTTCCAC GGCTGCATCC GGAACCTGTA TATCAACAGC 
GAGCTCCAGG ACTTCCGCCA GGTGCCCATG CAGACCGGCA TCCTGCCCGG CTGCGAGCCG 
TGCCACAGGA AGGTGTGTGC CCACGGCGCG TGCCAGCCCA GCAGCCAGTC GGGCTTCACC 
TGCGAGTGCG AGGAGGGCTG GACGGGGCCC CTGTGTGACC AGAGGACCAA CGACCCCTGT 
CTCGGGAACA ACTGTAAGTG TCTGGAGGGC CACGGGGGCG TCCTCTGCGA CGAAGAGGAG 
GACCTGTTCA ACCCCTGCCA GGCCATCAGG TGCAAGCACG GGAAATGCAG GCTCTCGGGC 
CTGGGCCAGC CCTACTGCGA ATGCAGCAGC GGGTACACGG GGGATAGCTG CGACCGAGAA 
GTGTCCTGTC GGGGCGAGCG CGTCCGGGAC TACTACCCAA AGCAGCAGGG CTACGCGGCC 
TGCCAGACCA CCAAGAAGGT GTCGCGGCTG GAGTGCAGGG GCGGCTGCGC GGCCGGGCAG 
TGCTGCGGGC CGCTGCGGAG CAAGCGGCGG AAATACTCCT TCGAGTGCAC GGACGGCTCG 
TCGTTCGTGG ACGAGGTGGA GAAGGTGGTC AAGTGCGGCT GCAGCAGGTG CGCCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SLIT2
Entrez Gene ID	595148
Full Name	slit guidance ligand 2
General protein information	Preferred Names slit guidance ligand 2 Names slit homolog 2 protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	SLIT2	XP_002808451.1
Canis lupus familiaris (dog)	SLIT2	XP_005618806.1
Danio rerio (zebrafish)	slit2	NP_571810.1
Xenopus tropicalis (tropical clawed frog)	slit2	XP_004911285.1
Homo sapiens (human)	SLIT2	NP_004778.1
Pan troglodytes (chimpanzee)	SLIT2	XP_003950343.1
Gallus gallus (chicken)	SLIT2	NP_001254004.1
Caenorhabditis elegans (roundworm)	slt-1	NP_510437.2
Bos taurus (cattle)	SLIT2	NP_001178445.2
Mus musculus (house mouse)	Slit2	NP_848919.3
Rattus norvegicus (Norway rat)	Slit2	NP_072154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002793	XP_312121.5
Drosophila melanogaster (fruit fly)	sli	NP_001261017.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SLIT2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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