SLIT2 cDNA ORF clone, Gallus gallus(chicken)

The following SLIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa149977 XM_025149454.1
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa149978 XM_025149455.1
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OGa149972 XM_025149450.1
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa149973 XM_025149451.1
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa149974 XM_015285716.2
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa149975 XM_025149452.1
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa149976 XM_025149453.1
Latest version!
Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa35677 XM_015285716.1
Latest version!
Gallus gallus slit homolog 2 (Drosophila) (SLIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa23967 NM_001267075.1
Latest version!
Gallus gallus slit homolog 2 (Drosophila) (SLIT2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa149977
    Clone ID Related Accession (Same CDS sequence) XM_025149454.1
    Accession Version XM_025149454.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA GGACATGGGG GAGTCCTCTG TGATGAAGAG GAAATGCTGT
    TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA GGACTTGGGA
    AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA GCCAAATCCA
    ATGATTCTGC TCCTAGTTTT ATCACAAACT CTACTTTTGA CCTCTCAGCT TCAACTGATT
    TATCACAGCG AATTATGCAG AAATCTCTTG TCGAGGGGAA CGAATCCGAG ATTACTACCA
    AAAGCAGCAA GGGTATGCTG CGTGCCAGAC GACCAAGAAG GTATCGAGAC TAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005222.1
    CDS27..4526
    Translation

    Target ORF information:

    RefSeq Version XM_025149454.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149454.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA GGACATGGGG GAGTCCTCTG TGATGAAGAG GAAATGCTGT
    TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA GGACTTGGGA
    AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA GCCAAATCCA
    ATGATTCTGC TCCTAGTTTT ATCACAAACT CTACTTTTGA CCTCTCAGCT TCAACTGATT
    TATCACAGCG AATTATGCAG AAATCTCTTG TCGAGGGGAA CGAATCCGAG ATTACTACCA
    AAAGCAGCAA GGGTATGCTG CGTGCCAGAC GACCAAGAAG GTATCGAGAC TAGAATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149978
    Clone ID Related Accession (Same CDS sequence) XM_025149455.1
    Accession Version XM_025149455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4404bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA GGACATGGGG GAGTCCTCTG TGATGAAGAG GAAATGCTGT
    TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA GGACTTGGGA
    AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA GAAATCTCTT
    GTCGAGGGGA ACGAATCCGA GATTACTACC AAAAGCAGCA AGGGTATGCT GCGTGCCAGA
    CGACCAAGAA GGTATCGAGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005223.1
    CDS27..4430
    Translation

    Target ORF information:

    RefSeq Version XM_025149455.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA GGACATGGGG GAGTCCTCTG TGATGAAGAG GAAATGCTGT
    TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA GGACTTGGGA
    AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA GAAATCTCTT
    GTCGAGGGGA ACGAATCCGA GATTACTACC AAAAGCAGCA AGGGTATGCT GCGTGCCAGA
    CGACCAAGAA GGTATCGAGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149972
    Clone ID Related Accession (Same CDS sequence) XM_025149450.1
    Accession Version XM_025149450.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA ATGTGTGCAT GGTACCTGCT TGCCGATCAA TGCATTTTCA
    TACAGTTGTA AATGCCTGCA GGGACATGGG GGAGTCCTCT GTGATGAAGA GGAAATGCTG
    TTTAACCCCT GCCAATCCAT CAGGTGTAAA CATGGCAAAT GCAGGCTTTC AGGACTTGGG
    AAACCATATT GCGAATGCAG CAGCGGATAC ACGGGGGACA GCTGTGATAA AGAAATCTCT
    TGTCGAGGGG AACGAATCCG AGATTACTAC CAAAAGCAGC AAGGGTATGC TGCGTGCCAG
    ACGACCAAGA AGGTATCGAG ACTAGAATGT AAAGGAGGAT GTTCAACCGG GCAGTGCTGT
    GGACCACTAA GGAGCAAGAG ACGGAAATAC TCTTTTGAAT GCACTGATGG GTCGTCATTT
    GTGGACGAGA TTGAAAAAGT GGTGAAGTGT GGCTGTACAA ATTGTCCCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005218.1
    CDS27..4640
    Translation

    Target ORF information:

    RefSeq Version XM_025149450.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA ATGTGTGCAT GGTACCTGCT TGCCGATCAA TGCATTTTCA
    TACAGTTGTA AATGCCTGCA GGGACATGGG GGAGTCCTCT GTGATGAAGA GGAAATGCTG
    TTTAACCCCT GCCAATCCAT CAGGTGTAAA CATGGCAAAT GCAGGCTTTC AGGACTTGGG
    AAACCATATT GCGAATGCAG CAGCGGATAC ACGGGGGACA GCTGTGATAA AGAAATCTCT
    TGTCGAGGGG AACGAATCCG AGATTACTAC CAAAAGCAGC AAGGGTATGC TGCGTGCCAG
    ACGACCAAGA AGGTATCGAG ACTAGAATGT AAAGGAGGAT GTTCAACCGG GCAGTGCTGT
    GGACCACTAA GGAGCAAGAG ACGGAAATAC TCTTTTGAAT GCACTGATGG GTCGTCATTT
    GTGGACGAGA TTGAAAAAGT GGTGAAGTGT GGCTGTACAA ATTGTCCCTC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149973
    Clone ID Related Accession (Same CDS sequence) XM_025149451.1
    Accession Version XM_025149451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGGCACT
    GAAGATTACA GATCCAAATT AAGTGGTGAC TGCTTTGCAG ATTTGGCTTG CCCTGAGAAA
    TGTCGCTGTG AAGGGACCAC AGTGGACTGC TCCAATCAGA AACTCAACAA AATTCCTGAT
    CACATCCCAC AGTACACAGC AGAGTTGCGA CTCAATAACA ATGAATTTTC AGTCCTGGAA
    GCTACTGGGA TCTTTAAGAA GCTTCCTCAA CTGCGAAAAA TAAACCTGAG CAATAACAAG
    ATTACAGATA TTGAAGAAGG TGCATTTGAT GGAGCCTCTG GTGTCAATGA ACTATTGCTC
    ACTAGCAATC GTTTGGAAAC TGTTAGAGAC AAAATGTTCA AAGGACTGGA AAGTCTTAAA
    ACACTGATGC TGAGGAGTAA CCGTGTGAGC TGTGTGGGGA ACGACAGTTT CACAGGCCTG
    AGCTCTGTCC GTCTGCTCTC ACTATATGAC AACCAGATCA CCACCGTGGC ACCCGGCTCC
    TTCGATACCC TGCATTCACT CTCTACATTA AACCTCTTGG CCAATCCTTT CAACTGCAAC
    TGCCATCTTG CATGGCTTGG AGATTGGCTA AGGAAGAAAC GCATTGTGAC GGGAAACCCT
    CGCTGTCAGA AACCTTATTT CCTCAAAGAG ATTCCTATCC AGGATGTGGC AATTCAGGAT
    TTTACATGTG ATGATGGAAA TGATGACAAT AGCTGCTCTC CGCTGTCCCG CTGTCCTGCA
    GAATGTACTT GTCTAGACAC AGTTGTTCGC TGCAGCAACA AAGGCCTAAA AGCTTTGCCT
    AAAGGCATCC CAAAAGATGT AACTGAACTA TATTTGGATG GAAACCAGTT TACTCTTGTT
    CCTAAAGAGC TCTCCAACTA CAAACATTTA ACACTTATAG ATTTAAGTAA CAACAGAATC
    AGCACTCTTT CTAATCAGAG CTTCAGCAAC ATGACTCAGC TGCTCACCTT AATTCTTAGT
    TACAACCGCC TGAGGTGTAT CCCTGCACGG ACTTTTGATG GGTTGAAATC ACTTAGGTTG
    CTGTCTTTAC ATGGCAATGA TATTTCTGTG GTTCCTGAAG GAGCCTTTAA TGATCTTTCA
    GCGTTATCAC ACCTGGCTAT TGGAGCAAAT CCTCTTTATT GTGATTGTAA CATGCAATGG
    CTGTCTGACT GGGTAAAATC AGAATACAAA GAACCTGGTA TTGCACGATG TGCTGGCCCT
    GGAGAAATGG CAGATAAACT TCTACTTACA ACTCCATCTA AAAAATTTAC TTGCCAAGGG
    CCCGTGGATG TCAATATTCT TGCTAAGTGT AACCCCTGCT TATCAAATCC ATGTAAAAAT
    GATGGAACCT GCAATAATGA TCCAGTTGAC TTCTATAGAT GTACTTGCCC ATATGGTTTC
    AAGGGTCAAG ACTGTGATAT TCCCATTCAT GCCTGCATTA GTAACCCTTG CAACCATGGT
    GGAACTTGTC ATTTGAAAGA AGGAGAAAAA GATGGTTTCT GGTGCACTTG TGCAGATGGA
    TTTGAAGGAG AAAATTGTGA AATAAATGTT GATGACTGTG AAGACAATGA CTGTGAAAAT
    AACTCTACTT GTGTGGATGG AATTAATAAT TATACTTGCC TTTGTCCACC TGAATATACA
    GCTGCTAATC TGAATGAAGT GGAAAAAGGT GAGCTCTGTG AGGAGAAACT AGATTTCTGT
    GCTCAAAACC TGAACCCTTG CCAGCACGAC TCAAAGTGTA TCTTGACTCC CAAAGGTTAC
    AAGTGTGATT GCACACCTGG ATATGTAGGT GAACACTGCG ATATTGACTT CGATGACTGC
    CAGGACAATA AATGTAAAAA CGGAGCACAG TGTACGGATG CAGTTAACGG GTATACTTGT
    ATTTGCCCAG AGGGATACAG TGGCTTGTTT TGTGAGTTTT CGCCACCAAT GGTTTTACCT
    CGCACCAGCC CTTGTGATAA TTATGAATGC CAAAATGGAG CCCAGTGTAT TGTAAAGGAG
    AGTGAACCAA TCTGCCAGTG TTTATCAGGC TACCAGGGTG AGAAATGTGA AAAGCTGATC
    AGTATAAACT TTGTCAACAA AGAATCCTAT CTACAAATCC CTTCAGCTAA GATACACTCC
    CAAACCAATA TCACTCTTCA GATTGCCACA GACGAAGACA GTGGGATCCT GCTCTACAAA
    GGCGATAAGG ATCATATAGC AGTAGAGCTG TACCGTGGTA GAGTGAGGGT CAGTTATGAC
    ACAGGATCTT ATCCAGCCTC TGCTATTTAC AGTGTGGAAA CTATTAATGA TGGCAATTTC
    CACATTGTGG AGCTGCTTGC CATGGATCAG ATTCTGTCTT TGTCTATTGA TGGAGGAAGC
    CCCAAGATAA TTACCAATTT GTCCAAGCAG TCCACTTTGA ATTTTGATTC TCCACTGTAT
    GTCGGAGGCA TGCCTGTGAA AAATAACATT GCAGCTCTAC GTCAGTCTCC AGGACAGAAT
    GGCACAAGCT TCCATGGCTG CATCCGTAAT CTGTATATCA ACAGCGAACT CCAGGACTTC
    AGAAATGTGC CACTGCAAGT GGGAATTCTG CCAGGTTGCG AGCCTTGTCA CAAGAAAGTT
    TGTGTGCATG GAACATGCCA TGCTACCAGC CAGTCAAGCT TTACCTGTGA GTGTGAAGGA
    GGATGGACTG GACCCCTCTG TGATCAACAA ACTAATGACC CGTGTCTCGG AAATAAATGT
    GTGCATGGTA CCTGCTTGCC GATCAATGCA TTTTCATACA GTTGTAAATG CCTGCAGGGA
    CATGGGGGAG TCCTCTGTGA TGAAGAGGAA ATGCTGTTTA ACCCCTGCCA ATCCATCAGG
    TGTAAACATG GCAAATGCAG GCTTTCAGGA CTTGGGAAAC CATATTGCGA ATGCAGCAGC
    GGATACACGG GGGACAGCTG TGATAAAGAA ATCTCTTGTC GAGGGGAACG AATCCGAGAT
    TACTACCAAA AGCAGCAAGG GTATGCTGCG TGCCAGACGA CCAAGAAGGT ATCGAGACTA
    GAATGTAAAG GAGGATGTTC AACCGGGCAG TGCTGTGGAC CACTAAGGAG CAAGAGACGG
    AAATACTCTT TTGAATGCAC TGATGGGTCG TCATTTGTGG ACGAGATTGA AAAAGTGGTG
    AAGTGTGGCT GTACAAATTG TCCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005219.1
    CDS27..4616
    Translation

    Target ORF information:

    RefSeq Version XM_025149451.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGGCACT
    GAAGATTACA GATCCAAATT AAGTGGTGAC TGCTTTGCAG ATTTGGCTTG CCCTGAGAAA
    TGTCGCTGTG AAGGGACCAC AGTGGACTGC TCCAATCAGA AACTCAACAA AATTCCTGAT
    CACATCCCAC AGTACACAGC AGAGTTGCGA CTCAATAACA ATGAATTTTC AGTCCTGGAA
    GCTACTGGGA TCTTTAAGAA GCTTCCTCAA CTGCGAAAAA TAAACCTGAG CAATAACAAG
    ATTACAGATA TTGAAGAAGG TGCATTTGAT GGAGCCTCTG GTGTCAATGA ACTATTGCTC
    ACTAGCAATC GTTTGGAAAC TGTTAGAGAC AAAATGTTCA AAGGACTGGA AAGTCTTAAA
    ACACTGATGC TGAGGAGTAA CCGTGTGAGC TGTGTGGGGA ACGACAGTTT CACAGGCCTG
    AGCTCTGTCC GTCTGCTCTC ACTATATGAC AACCAGATCA CCACCGTGGC ACCCGGCTCC
    TTCGATACCC TGCATTCACT CTCTACATTA AACCTCTTGG CCAATCCTTT CAACTGCAAC
    TGCCATCTTG CATGGCTTGG AGATTGGCTA AGGAAGAAAC GCATTGTGAC GGGAAACCCT
    CGCTGTCAGA AACCTTATTT CCTCAAAGAG ATTCCTATCC AGGATGTGGC AATTCAGGAT
    TTTACATGTG ATGATGGAAA TGATGACAAT AGCTGCTCTC CGCTGTCCCG CTGTCCTGCA
    GAATGTACTT GTCTAGACAC AGTTGTTCGC TGCAGCAACA AAGGCCTAAA AGCTTTGCCT
    AAAGGCATCC CAAAAGATGT AACTGAACTA TATTTGGATG GAAACCAGTT TACTCTTGTT
    CCTAAAGAGC TCTCCAACTA CAAACATTTA ACACTTATAG ATTTAAGTAA CAACAGAATC
    AGCACTCTTT CTAATCAGAG CTTCAGCAAC ATGACTCAGC TGCTCACCTT AATTCTTAGT
    TACAACCGCC TGAGGTGTAT CCCTGCACGG ACTTTTGATG GGTTGAAATC ACTTAGGTTG
    CTGTCTTTAC ATGGCAATGA TATTTCTGTG GTTCCTGAAG GAGCCTTTAA TGATCTTTCA
    GCGTTATCAC ACCTGGCTAT TGGAGCAAAT CCTCTTTATT GTGATTGTAA CATGCAATGG
    CTGTCTGACT GGGTAAAATC AGAATACAAA GAACCTGGTA TTGCACGATG TGCTGGCCCT
    GGAGAAATGG CAGATAAACT TCTACTTACA ACTCCATCTA AAAAATTTAC TTGCCAAGGG
    CCCGTGGATG TCAATATTCT TGCTAAGTGT AACCCCTGCT TATCAAATCC ATGTAAAAAT
    GATGGAACCT GCAATAATGA TCCAGTTGAC TTCTATAGAT GTACTTGCCC ATATGGTTTC
    AAGGGTCAAG ACTGTGATAT TCCCATTCAT GCCTGCATTA GTAACCCTTG CAACCATGGT
    GGAACTTGTC ATTTGAAAGA AGGAGAAAAA GATGGTTTCT GGTGCACTTG TGCAGATGGA
    TTTGAAGGAG AAAATTGTGA AATAAATGTT GATGACTGTG AAGACAATGA CTGTGAAAAT
    AACTCTACTT GTGTGGATGG AATTAATAAT TATACTTGCC TTTGTCCACC TGAATATACA
    GCTGCTAATC TGAATGAAGT GGAAAAAGGT GAGCTCTGTG AGGAGAAACT AGATTTCTGT
    GCTCAAAACC TGAACCCTTG CCAGCACGAC TCAAAGTGTA TCTTGACTCC CAAAGGTTAC
    AAGTGTGATT GCACACCTGG ATATGTAGGT GAACACTGCG ATATTGACTT CGATGACTGC
    CAGGACAATA AATGTAAAAA CGGAGCACAG TGTACGGATG CAGTTAACGG GTATACTTGT
    ATTTGCCCAG AGGGATACAG TGGCTTGTTT TGTGAGTTTT CGCCACCAAT GGTTTTACCT
    CGCACCAGCC CTTGTGATAA TTATGAATGC CAAAATGGAG CCCAGTGTAT TGTAAAGGAG
    AGTGAACCAA TCTGCCAGTG TTTATCAGGC TACCAGGGTG AGAAATGTGA AAAGCTGATC
    AGTATAAACT TTGTCAACAA AGAATCCTAT CTACAAATCC CTTCAGCTAA GATACACTCC
    CAAACCAATA TCACTCTTCA GATTGCCACA GACGAAGACA GTGGGATCCT GCTCTACAAA
    GGCGATAAGG ATCATATAGC AGTAGAGCTG TACCGTGGTA GAGTGAGGGT CAGTTATGAC
    ACAGGATCTT ATCCAGCCTC TGCTATTTAC AGTGTGGAAA CTATTAATGA TGGCAATTTC
    CACATTGTGG AGCTGCTTGC CATGGATCAG ATTCTGTCTT TGTCTATTGA TGGAGGAAGC
    CCCAAGATAA TTACCAATTT GTCCAAGCAG TCCACTTTGA ATTTTGATTC TCCACTGTAT
    GTCGGAGGCA TGCCTGTGAA AAATAACATT GCAGCTCTAC GTCAGTCTCC AGGACAGAAT
    GGCACAAGCT TCCATGGCTG CATCCGTAAT CTGTATATCA ACAGCGAACT CCAGGACTTC
    AGAAATGTGC CACTGCAAGT GGGAATTCTG CCAGGTTGCG AGCCTTGTCA CAAGAAAGTT
    TGTGTGCATG GAACATGCCA TGCTACCAGC CAGTCAAGCT TTACCTGTGA GTGTGAAGGA
    GGATGGACTG GACCCCTCTG TGATCAACAA ACTAATGACC CGTGTCTCGG AAATAAATGT
    GTGCATGGTA CCTGCTTGCC GATCAATGCA TTTTCATACA GTTGTAAATG CCTGCAGGGA
    CATGGGGGAG TCCTCTGTGA TGAAGAGGAA ATGCTGTTTA ACCCCTGCCA ATCCATCAGG
    TGTAAACATG GCAAATGCAG GCTTTCAGGA CTTGGGAAAC CATATTGCGA ATGCAGCAGC
    GGATACACGG GGGACAGCTG TGATAAAGAA ATCTCTTGTC GAGGGGAACG AATCCGAGAT
    TACTACCAAA AGCAGCAAGG GTATGCTGCG TGCCAGACGA CCAAGAAGGT ATCGAGACTA
    GAATGTAAAG GAGGATGTTC AACCGGGCAG TGCTGTGGAC CACTAAGGAG CAAGAGACGG
    AAATACTCTT TTGAATGCAC TGATGGGTCG TCATTTGTGG ACGAGATTGA AAAAGTGGTG
    AAGTGTGGCT GTACAAATTG TCCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149974
    Clone ID Related Accession (Same CDS sequence) XM_015285716.2
    Accession Version XM_015285716.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGGCACT
    GAAGATTACA GATCCAAATT AAGTGGTGAC TGCTTTGCAG ATTTGGCTTG CCCTGAGAAA
    TGTCGCTGTG AAGGGACCAC AGTGGACTGC TCCAATCAGA AACTCAACAA AATTCCTGAT
    CACATCCCAC AGTACACAGC AGAGTTGCGA CTCAATAACA ATGAATTTTC AGTCCTGGAA
    GCTACTGGGA TCTTTAAGAA GCTTCCTCAA CTGCGAAAAA TAAACCTGAG CAATAACAAG
    ATTACAGATA TTGAAGAAGG TGCATTTGAT GGAGCCTCTG GTGTCAATGA ACTATTGCTC
    ACTAGCAATC GTTTGGAAAC TGTTAGAGAC AAAATGTTCA AAGGACTGGA AAGTCTTAAA
    ACACTGATGC TGAGGAGTAA CCGTGTGAGC TGTGTGGGGA ACGACAGTTT CACAGGCCTG
    AGCTCTGTCC GTCTGCTCTC ACTATATGAC AACCAGATCA CCACCGTGGC ACCCGGCTCC
    TTCGATACCC TGCATTCACT CTCTACATTA AACCTCTTGG CCAATCCTTT CAACTGCAAC
    TGCCATCTTG CATGGCTTGG AGATTGGCTA AGGAAGAAAC GCATTGTGAC GGGAAACCCT
    CGCTGTCAGA AACCTTATTT CCTCAAAGAG ATTCCTATCC AGGATGTGGC AATTCAGGAT
    TTTACATGTG ATGATGGAAA TGATGACAAT AGCTGCTCTC CGCTGTCCCG CTGTCCTGCA
    GAATGTACTT GTCTAGACAC AGTTGTTCGC TGCAGCAACA AAGGCCTAAA AGCTTTGCCT
    AAAGGCATCC CAAAAGATGT AACTGAACTA TATTTGGATG GAAACCAGTT TACTCTTGTT
    CCTAAAGAGC TCTCCAACTA CAAACATTTA ACACTTATAG ATTTAAGTAA CAACAGAATC
    AGCACTCTTT CTAATCAGAG CTTCAGCAAC ATGACTCAGC TGCTCACCTT AATTCTTAGT
    TACAACCGCC TGAGGTGTAT CCCTGCACGG ACTTTTGATG GGTTGAAATC ACTTAGGTTG
    CTGTCTTTAC ATGGCAATGA TATTTCTGTG GTTCCTGAAG GAGCCTTTAA TGATCTTTCA
    GCGTTATCAC ACCTGGCTAT TGGAGCAAAT CCTCTTTATT GTGATTGTAA CATGCAATGG
    CTGTCTGACT GGGTAAAATC AGAATACAAA GAACCTGGTA TTGCACGATG TGCTGGCCCT
    GGAGAAATGG CAGATAAACT TCTACTTACA ACTCCATCTA AAAAATTTAC TTGCCAAGGG
    CCCGTGGATG TCAATATTCT TGCTAAGTGT AACCCCTGCT TATCAAATCC ATGTAAAAAT
    GATGGAACCT GCAATAATGA TCCAGTTGAC TTCTATAGAT GTACTTGCCC ATATGGTTTC
    AAGGGTCAAG ACTGTGATAT TCCCATTCAT GCCTGCATTA GTAACCCTTG CAACCATGGT
    GGAACTTGTC ATTTGAAAGA AGGAGAAAAA GATGGTTTCT GGTGCACTTG TGCAGATGGA
    TTTGAAGGAG AAAATTGTGA AATAAATGTT GATGACTGTG AAGACAATGA CTGTGAAAAT
    AACTCTACTT GTGTGGATGG AATTAATAAT TATACTTGCC TTTGTCCACC TGAATATACA
    GGTGAGCTCT GTGAGGAGAA ACTAGATTTC TGTGCTCAAA ACCTGAACCC TTGCCAGCAC
    GACTCAAAGT GTATCTTGAC TCCCAAAGGT TACAAGTGTG ATTGCACACC TGGATATGTA
    GGTGAACACT GCGATATTGA CTTCGATGAC TGCCAGGACA ATAAATGTAA AAACGGAGCA
    CAGTGTACGG ATGCAGTTAA CGGGTATACT TGTATTTGCC CAGAGGGATA CAGTGGCTTG
    TTTTGTGAGT TTTCGCCACC AATGGTTTTA CCTCGCACCA GCCCTTGTGA TAATTATGAA
    TGCCAAAATG GAGCCCAGTG TATTGTAAAG GAGAGTGAAC CAATCTGCCA GTGTTTATCA
    GGCTACCAGG GTGAGAAATG TGAAAAGCTG ATCAGTATAA ACTTTGTCAA CAAAGAATCC
    TATCTACAAA TCCCTTCAGC TAAGATACAC TCCCAAACCA ATATCACTCT TCAGATTGCC
    ACAGACGAAG ACAGTGGGAT CCTGCTCTAC AAAGGCGATA AGGATCATAT AGCAGTAGAG
    CTGTACCGTG GTAGAGTGAG GGTCAGTTAT GACACAGGAT CTTATCCAGC CTCTGCTATT
    TACAGTGTGG AAACTATTAA TGATGGCAAT TTCCACATTG TGGAGCTGCT TGCCATGGAT
    CAGATTCTGT CTTTGTCTAT TGATGGAGGA AGCCCCAAGA TAATTACCAA TTTGTCCAAG
    CAGTCCACTT TGAATTTTGA TTCTCCACTG TATGTCGGAG GCATGCCTGT GAAAAATAAC
    ATTGCAGCTC TACGTCAGTC TCCAGGACAG AATGGCACAA GCTTCCATGG CTGCATCCGT
    AATCTGTATA TCAACAGCGA ACTCCAGGAC TTCAGAAATG TGCCACTGCA AGTGGGAATT
    CTGCCAGGTT GCGAGCCTTG TCACAAGAAA GTTTGTGTGC ATGGAACATG CCATGCTACC
    AGCCAGTCAA GCTTTACCTG TGAGTGTGAA GGAGGATGGA CTGGACCCCT CTGTGATCAA
    CAAACTAATG ACCCGTGTCT CGGAAATAAA TGTGTGCATG GTACCTGCTT GCCGATCAAT
    GCATTTTCAT ACAGTTGTAA ATGCCTGCAG GGACATGGGG GAGTCCTCTG TGATGAAGAG
    GAAATGCTGT TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA
    GGACTTGGGA AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA
    GAAATCTCTT GTCGAGGGGA ACGAATCCGA GATTACTACC AAAAGCAGCA AGGGTATGCT
    GCGTGCCAGA CGACCAAGAA GGTATCGAGA CTAGAATGTA AAGGAGGATG TTCAACCGGG
    CAGTGCTGTG GACCACTAAG GAGCAAGAGA CGGAAATACT CTTTTGAATG CACTGATGGG
    TCGTCATTTG TGGACGAGAT TGAAAAAGTG GTGAAGTGTG GCTGTACAAA TTGTCCCTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141202.2
    CDS27..4589
    Translation

    Target ORF information:

    RefSeq Version XM_015285716.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285716.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGGCACT
    GAAGATTACA GATCCAAATT AAGTGGTGAC TGCTTTGCAG ATTTGGCTTG CCCTGAGAAA
    TGTCGCTGTG AAGGGACCAC AGTGGACTGC TCCAATCAGA AACTCAACAA AATTCCTGAT
    CACATCCCAC AGTACACAGC AGAGTTGCGA CTCAATAACA ATGAATTTTC AGTCCTGGAA
    GCTACTGGGA TCTTTAAGAA GCTTCCTCAA CTGCGAAAAA TAAACCTGAG CAATAACAAG
    ATTACAGATA TTGAAGAAGG TGCATTTGAT GGAGCCTCTG GTGTCAATGA ACTATTGCTC
    ACTAGCAATC GTTTGGAAAC TGTTAGAGAC AAAATGTTCA AAGGACTGGA AAGTCTTAAA
    ACACTGATGC TGAGGAGTAA CCGTGTGAGC TGTGTGGGGA ACGACAGTTT CACAGGCCTG
    AGCTCTGTCC GTCTGCTCTC ACTATATGAC AACCAGATCA CCACCGTGGC ACCCGGCTCC
    TTCGATACCC TGCATTCACT CTCTACATTA AACCTCTTGG CCAATCCTTT CAACTGCAAC
    TGCCATCTTG CATGGCTTGG AGATTGGCTA AGGAAGAAAC GCATTGTGAC GGGAAACCCT
    CGCTGTCAGA AACCTTATTT CCTCAAAGAG ATTCCTATCC AGGATGTGGC AATTCAGGAT
    TTTACATGTG ATGATGGAAA TGATGACAAT AGCTGCTCTC CGCTGTCCCG CTGTCCTGCA
    GAATGTACTT GTCTAGACAC AGTTGTTCGC TGCAGCAACA AAGGCCTAAA AGCTTTGCCT
    AAAGGCATCC CAAAAGATGT AACTGAACTA TATTTGGATG GAAACCAGTT TACTCTTGTT
    CCTAAAGAGC TCTCCAACTA CAAACATTTA ACACTTATAG ATTTAAGTAA CAACAGAATC
    AGCACTCTTT CTAATCAGAG CTTCAGCAAC ATGACTCAGC TGCTCACCTT AATTCTTAGT
    TACAACCGCC TGAGGTGTAT CCCTGCACGG ACTTTTGATG GGTTGAAATC ACTTAGGTTG
    CTGTCTTTAC ATGGCAATGA TATTTCTGTG GTTCCTGAAG GAGCCTTTAA TGATCTTTCA
    GCGTTATCAC ACCTGGCTAT TGGAGCAAAT CCTCTTTATT GTGATTGTAA CATGCAATGG
    CTGTCTGACT GGGTAAAATC AGAATACAAA GAACCTGGTA TTGCACGATG TGCTGGCCCT
    GGAGAAATGG CAGATAAACT TCTACTTACA ACTCCATCTA AAAAATTTAC TTGCCAAGGG
    CCCGTGGATG TCAATATTCT TGCTAAGTGT AACCCCTGCT TATCAAATCC ATGTAAAAAT
    GATGGAACCT GCAATAATGA TCCAGTTGAC TTCTATAGAT GTACTTGCCC ATATGGTTTC
    AAGGGTCAAG ACTGTGATAT TCCCATTCAT GCCTGCATTA GTAACCCTTG CAACCATGGT
    GGAACTTGTC ATTTGAAAGA AGGAGAAAAA GATGGTTTCT GGTGCACTTG TGCAGATGGA
    TTTGAAGGAG AAAATTGTGA AATAAATGTT GATGACTGTG AAGACAATGA CTGTGAAAAT
    AACTCTACTT GTGTGGATGG AATTAATAAT TATACTTGCC TTTGTCCACC TGAATATACA
    GGTGAGCTCT GTGAGGAGAA ACTAGATTTC TGTGCTCAAA ACCTGAACCC TTGCCAGCAC
    GACTCAAAGT GTATCTTGAC TCCCAAAGGT TACAAGTGTG ATTGCACACC TGGATATGTA
    GGTGAACACT GCGATATTGA CTTCGATGAC TGCCAGGACA ATAAATGTAA AAACGGAGCA
    CAGTGTACGG ATGCAGTTAA CGGGTATACT TGTATTTGCC CAGAGGGATA CAGTGGCTTG
    TTTTGTGAGT TTTCGCCACC AATGGTTTTA CCTCGCACCA GCCCTTGTGA TAATTATGAA
    TGCCAAAATG GAGCCCAGTG TATTGTAAAG GAGAGTGAAC CAATCTGCCA GTGTTTATCA
    GGCTACCAGG GTGAGAAATG TGAAAAGCTG ATCAGTATAA ACTTTGTCAA CAAAGAATCC
    TATCTACAAA TCCCTTCAGC TAAGATACAC TCCCAAACCA ATATCACTCT TCAGATTGCC
    ACAGACGAAG ACAGTGGGAT CCTGCTCTAC AAAGGCGATA AGGATCATAT AGCAGTAGAG
    CTGTACCGTG GTAGAGTGAG GGTCAGTTAT GACACAGGAT CTTATCCAGC CTCTGCTATT
    TACAGTGTGG AAACTATTAA TGATGGCAAT TTCCACATTG TGGAGCTGCT TGCCATGGAT
    CAGATTCTGT CTTTGTCTAT TGATGGAGGA AGCCCCAAGA TAATTACCAA TTTGTCCAAG
    CAGTCCACTT TGAATTTTGA TTCTCCACTG TATGTCGGAG GCATGCCTGT GAAAAATAAC
    ATTGCAGCTC TACGTCAGTC TCCAGGACAG AATGGCACAA GCTTCCATGG CTGCATCCGT
    AATCTGTATA TCAACAGCGA ACTCCAGGAC TTCAGAAATG TGCCACTGCA AGTGGGAATT
    CTGCCAGGTT GCGAGCCTTG TCACAAGAAA GTTTGTGTGC ATGGAACATG CCATGCTACC
    AGCCAGTCAA GCTTTACCTG TGAGTGTGAA GGAGGATGGA CTGGACCCCT CTGTGATCAA
    CAAACTAATG ACCCGTGTCT CGGAAATAAA TGTGTGCATG GTACCTGCTT GCCGATCAAT
    GCATTTTCAT ACAGTTGTAA ATGCCTGCAG GGACATGGGG GAGTCCTCTG TGATGAAGAG
    GAAATGCTGT TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA
    GGACTTGGGA AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA
    GAAATCTCTT GTCGAGGGGA ACGAATCCGA GATTACTACC AAAAGCAGCA AGGGTATGCT
    GCGTGCCAGA CGACCAAGAA GGTATCGAGA CTAGAATGTA AAGGAGGATG TTCAACCGGG
    CAGTGCTGTG GACCACTAAG GAGCAAGAGA CGGAAATACT CTTTTGAATG CACTGATGGG
    TCGTCATTTG TGGACGAGAT TGAAAAAGTG GTGAAGTGTG GCTGTACAAA TTGTCCCTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149975
    Clone ID Related Accession (Same CDS sequence) XM_025149452.1
    Accession Version XM_025149452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA ATGTGTGCAT GGTACCTGCT TGCCGATCAA TGCATTTTCA
    TACAGTTGTA AATGCCTGCA GGGACATGGG GGAGTCCTCT GTGATGAAGA GGAAATGCTG
    TTTAACCCCT GCCAATCCAT CAGGTGTAAA CATGGCAAAT GCAGGCTTTC AGGACTTGGG
    AAACCATATT GCGAATGCAG CAGCGGATAC ACGGGGGACA GCTGTGATAA AGCCAAATCC
    AATGATTCTG CTCCTAGTTT TATCACAAAC TCTACTTTTG ACCTCTCAGC TTCAACTGAT
    TTATCACAGC GAATTATGCA GAAATCTCTT GTCGAGGGGA ACGAATCCGA GATTACTACC
    AAAAGCAGCA AGGGTATGCT GCGTGCCAGA CGACCAAGAA GGTATCGAGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005220.1
    CDS27..4580
    Translation

    Target ORF information:

    RefSeq Version XM_025149452.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGCTGCT AATCTGAATG AAGTGGAAAA AGGTGAGCTC
    TGTGAGGAGA AACTAGATTT CTGTGCTCAA AACCTGAACC CTTGCCAGCA CGACTCAAAG
    TGTATCTTGA CTCCCAAAGG TTACAAGTGT GATTGCACAC CTGGATATGT AGGTGAACAC
    TGCGATATTG ACTTCGATGA CTGCCAGGAC AATAAATGTA AAAACGGAGC ACAGTGTACG
    GATGCAGTTA ACGGGTATAC TTGTATTTGC CCAGAGGGAT ACAGTGGCTT GTTTTGTGAG
    TTTTCGCCAC CAATGGTTTT ACCTCGCACC AGCCCTTGTG ATAATTATGA ATGCCAAAAT
    GGAGCCCAGT GTATTGTAAA GGAGAGTGAA CCAATCTGCC AGTGTTTATC AGGCTACCAG
    GGTGAGAAAT GTGAAAAGCT GATCAGTATA AACTTTGTCA ACAAAGAATC CTATCTACAA
    ATCCCTTCAG CTAAGATACA CTCCCAAACC AATATCACTC TTCAGATTGC CACAGACGAA
    GACAGTGGGA TCCTGCTCTA CAAAGGCGAT AAGGATCATA TAGCAGTAGA GCTGTACCGT
    GGTAGAGTGA GGGTCAGTTA TGACACAGGA TCTTATCCAG CCTCTGCTAT TTACAGTGTG
    GAAACTATTA ATGATGGCAA TTTCCACATT GTGGAGCTGC TTGCCATGGA TCAGATTCTG
    TCTTTGTCTA TTGATGGAGG AAGCCCCAAG ATAATTACCA ATTTGTCCAA GCAGTCCACT
    TTGAATTTTG ATTCTCCACT GTATGTCGGA GGCATGCCTG TGAAAAATAA CATTGCAGCT
    CTACGTCAGT CTCCAGGACA GAATGGCACA AGCTTCCATG GCTGCATCCG TAATCTGTAT
    ATCAACAGCG AACTCCAGGA CTTCAGAAAT GTGCCACTGC AAGTGGGAAT TCTGCCAGGT
    TGCGAGCCTT GTCACAAGAA AGTTTGTGTG CATGGAACAT GCCATGCTAC CAGCCAGTCA
    AGCTTTACCT GTGAGTGTGA AGGAGGATGG ACTGGACCCC TCTGTGATCA ACAAACTAAT
    GACCCGTGTC TCGGAAATAA ATGTGTGCAT GGTACCTGCT TGCCGATCAA TGCATTTTCA
    TACAGTTGTA AATGCCTGCA GGGACATGGG GGAGTCCTCT GTGATGAAGA GGAAATGCTG
    TTTAACCCCT GCCAATCCAT CAGGTGTAAA CATGGCAAAT GCAGGCTTTC AGGACTTGGG
    AAACCATATT GCGAATGCAG CAGCGGATAC ACGGGGGACA GCTGTGATAA AGCCAAATCC
    AATGATTCTG CTCCTAGTTT TATCACAAAC TCTACTTTTG ACCTCTCAGC TTCAACTGAT
    TTATCACAGC GAATTATGCA GAAATCTCTT GTCGAGGGGA ACGAATCCGA GATTACTACC
    AAAAGCAGCA AGGGTATGCT GCGTGCCAGA CGACCAAGAA GGTATCGAGA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149976
    Clone ID Related Accession (Same CDS sequence) XM_025149453.1
    Accession Version XM_025149453.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4542bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006091.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAATTCTTA GTTACAACCG CCTGAGGTGT ATCCCTGCAC GGACTTTTGA TGGGTTGAAA
    TCACTTAGGT TGCTGTCTTT ACATGGCAAT GATATTTCTG TGGTTCCTGA AGGAGCCTTT
    AATGATCTTT CAGCGTTATC ACACCTGGCT ATTGGAGCAA ATCCTCTTTA TTGTGATTGT
    AACATGCAAT GGCTGTCTGA CTGGGTAAAA TCAGAATACA AAGAACCTGG TATTGCACGA
    TGTGCTGGCC CTGGAGAAAT GGCAGATAAA CTTCTACTTA CAACTCCATC TAAAAAATTT
    ACTTGCCAAG GGCCCGTGGA TGTCAATATT CTTGCTAAGT GTAACCCCTG CTTATCAAAT
    CCATGTAAAA ATGATGGAAC CTGCAATAAT GATCCAGTTG ACTTCTATAG ATGTACTTGC
    CCATATGGTT TCAAGGGTCA AGACTGTGAT ATTCCCATTC ATGCCTGCAT TAGTAACCCT
    TGCAACCATG GTGGAACTTG TCATTTGAAA GAAGGAGAAA AAGATGGTTT CTGGTGCACT
    TGTGCAGATG GATTTGAAGG AGAAAATTGT GAAATAAATG TTGATGACTG TGAAGACAAT
    GACTGTGAAA ATAACTCTAC TTGTGTGGAT GGAATTAATA ATTATACTTG CCTTTGTCCA
    CCTGAATATA CAGCTGCTAA TCTGAATGAA GTGGAAAAAG GTGAGCTCTG TGAGGAGAAA
    CTAGATTTCT GTGCTCAAAA CCTGAACCCT TGCCAGCACG ACTCAAAGTG TATCTTGACT
    CCCAAAGGTT ACAAGTGTGA TTGCACACCT GGATATGTAG GTGAACACTG CGATATTGAC
    TTCGATGACT GCCAGGACAA TAAATGTAAA AACGGAGCAC AGTGTACGGA TGCAGTTAAC
    GGGTATACTT GTATTTGCCC AGAGGGATAC AGTGGCTTGT TTTGTGAGTT TTCGCCACCA
    ATGGTTTTAC CTCGCACCAG CCCTTGTGAT AATTATGAAT GCCAAAATGG AGCCCAGTGT
    ATTGTAAAGG AGAGTGAACC AATCTGCCAG TGTTTATCAG GCTACCAGGG TGAGAAATGT
    GAAAAGCTGA TCAGTATAAA CTTTGTCAAC AAAGAATCCT ATCTACAAAT CCCTTCAGCT
    AAGATACACT CCCAAACCAA TATCACTCTT CAGATTGCCA CAGACGAAGA CAGTGGGATC
    CTGCTCTACA AAGGCGATAA GGATCATATA GCAGTAGAGC TGTACCGTGG TAGAGTGAGG
    GTCAGTTATG ACACAGGATC TTATCCAGCC TCTGCTATTT ACAGTGTGGA AACTATTAAT
    GATGGCAATT TCCACATTGT GGAGCTGCTT GCCATGGATC AGATTCTGTC TTTGTCTATT
    GATGGAGGAA GCCCCAAGAT AATTACCAAT TTGTCCAAGC AGTCCACTTT GAATTTTGAT
    TCTCCACTGT ATGTCGGAGG CATGCCTGTG AAAAATAACA TTGCAGCTCT ACGTCAGTCT
    CCAGGACAGA ATGGCACAAG CTTCCATGGC TGCATCCGTA ATCTGTATAT CAACAGCGAA
    CTCCAGGACT TCAGAAATGT GCCACTGCAA GTGGGAATTC TGCCAGGTTG CGAGCCTTGT
    CACAAGAAAG TTTGTGTGCA TGGAACATGC CATGCTACCA GCCAGTCAAG CTTTACCTGT
    GAGTGTGAAG GAGGATGGAC TGGACCCCTC TGTGATCAAC AAACTAATGA CCCGTGTCTC
    GGAAATAAAT GTGTGCATGG TACCTGCTTG CCGATCAATG CATTTTCATA CAGTTGTAAA
    TGCCTGCAGG GACATGGGGG AGTCCTCTGT GATGAAGAGG AAATGCTGTT TAACCCCTGC
    CAATCCATCA GGTGTAAACA TGGCAAATGC AGGCTTTCAG GACTTGGGAA ACCATATTGC
    GAATGCAGCA GCGGATACAC GGGGGACAGC TGTGATAAAG AAATCTCTTG TCGAGGGGAA
    CGAATCCGAG ATTACTACCA AAAGCAGCAA GGGTATGCTG CGTGCCAGAC GACCAAGAAG
    GTATCGAGAC TAGAATGTAA AGGAGGATGT TCAACCGGGC AGTGCTGTGG ACCACTAAGG
    AGCAAGAGAC GGAAATACTC TTTTGAATGC ACTGATGGGT CGTCATTTGT GGACGAGATT
    GAAAAAGTGG TGAAGTGTGG CTGTACAAAT TGTCCCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025005221.1
    CDS27..4568
    Translation

    Target ORF information:

    RefSeq Version XM_025149453.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit guidance ligand 2 (SLIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025149453.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACCGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTGC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAATTCTTA GTTACAACCG CCTGAGGTGT ATCCCTGCAC GGACTTTTGA TGGGTTGAAA
    TCACTTAGGT TGCTGTCTTT ACATGGCAAT GATATTTCTG TGGTTCCTGA AGGAGCCTTT
    AATGATCTTT CAGCGTTATC ACACCTGGCT ATTGGAGCAA ATCCTCTTTA TTGTGATTGT
    AACATGCAAT GGCTGTCTGA CTGGGTAAAA TCAGAATACA AAGAACCTGG TATTGCACGA
    TGTGCTGGCC CTGGAGAAAT GGCAGATAAA CTTCTACTTA CAACTCCATC TAAAAAATTT
    ACTTGCCAAG GGCCCGTGGA TGTCAATATT CTTGCTAAGT GTAACCCCTG CTTATCAAAT
    CCATGTAAAA ATGATGGAAC CTGCAATAAT GATCCAGTTG ACTTCTATAG ATGTACTTGC
    CCATATGGTT TCAAGGGTCA AGACTGTGAT ATTCCCATTC ATGCCTGCAT TAGTAACCCT
    TGCAACCATG GTGGAACTTG TCATTTGAAA GAAGGAGAAA AAGATGGTTT CTGGTGCACT
    TGTGCAGATG GATTTGAAGG AGAAAATTGT GAAATAAATG TTGATGACTG TGAAGACAAT
    GACTGTGAAA ATAACTCTAC TTGTGTGGAT GGAATTAATA ATTATACTTG CCTTTGTCCA
    CCTGAATATA CAGCTGCTAA TCTGAATGAA GTGGAAAAAG GTGAGCTCTG TGAGGAGAAA
    CTAGATTTCT GTGCTCAAAA CCTGAACCCT TGCCAGCACG ACTCAAAGTG TATCTTGACT
    CCCAAAGGTT ACAAGTGTGA TTGCACACCT GGATATGTAG GTGAACACTG CGATATTGAC
    TTCGATGACT GCCAGGACAA TAAATGTAAA AACGGAGCAC AGTGTACGGA TGCAGTTAAC
    GGGTATACTT GTATTTGCCC AGAGGGATAC AGTGGCTTGT TTTGTGAGTT TTCGCCACCA
    ATGGTTTTAC CTCGCACCAG CCCTTGTGAT AATTATGAAT GCCAAAATGG AGCCCAGTGT
    ATTGTAAAGG AGAGTGAACC AATCTGCCAG TGTTTATCAG GCTACCAGGG TGAGAAATGT
    GAAAAGCTGA TCAGTATAAA CTTTGTCAAC AAAGAATCCT ATCTACAAAT CCCTTCAGCT
    AAGATACACT CCCAAACCAA TATCACTCTT CAGATTGCCA CAGACGAAGA CAGTGGGATC
    CTGCTCTACA AAGGCGATAA GGATCATATA GCAGTAGAGC TGTACCGTGG TAGAGTGAGG
    GTCAGTTATG ACACAGGATC TTATCCAGCC TCTGCTATTT ACAGTGTGGA AACTATTAAT
    GATGGCAATT TCCACATTGT GGAGCTGCTT GCCATGGATC AGATTCTGTC TTTGTCTATT
    GATGGAGGAA GCCCCAAGAT AATTACCAAT TTGTCCAAGC AGTCCACTTT GAATTTTGAT
    TCTCCACTGT ATGTCGGAGG CATGCCTGTG AAAAATAACA TTGCAGCTCT ACGTCAGTCT
    CCAGGACAGA ATGGCACAAG CTTCCATGGC TGCATCCGTA ATCTGTATAT CAACAGCGAA
    CTCCAGGACT TCAGAAATGT GCCACTGCAA GTGGGAATTC TGCCAGGTTG CGAGCCTTGT
    CACAAGAAAG TTTGTGTGCA TGGAACATGC CATGCTACCA GCCAGTCAAG CTTTACCTGT
    GAGTGTGAAG GAGGATGGAC TGGACCCCTC TGTGATCAAC AAACTAATGA CCCGTGTCTC
    GGAAATAAAT GTGTGCATGG TACCTGCTTG CCGATCAATG CATTTTCATA CAGTTGTAAA
    TGCCTGCAGG GACATGGGGG AGTCCTCTGT GATGAAGAGG AAATGCTGTT TAACCCCTGC
    CAATCCATCA GGTGTAAACA TGGCAAATGC AGGCTTTCAG GACTTGGGAA ACCATATTGC
    GAATGCAGCA GCGGATACAC GGGGGACAGC TGTGATAAAG AAATCTCTTG TCGAGGGGAA
    CGAATCCGAG ATTACTACCA AAAGCAGCAA GGGTATGCTG CGTGCCAGAC GACCAAGAAG
    GTATCGAGAC TAGAATGTAA AGGAGGATGT TCAACCGGGC AGTGCTGTGG ACCACTAAGG
    AGCAAGAGAC GGAAATACTC TTTTGAATGC ACTGATGGGT CGTCATTTGT GGACGAGATT
    GAAAAAGTGG TGAAGTGTGG CTGTACAAAT TGTCCCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa35677
    Clone ID Related Accession (Same CDS sequence) XM_015285716.1
    Accession Version XM_015285716.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455862.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACTGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTAC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCA
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCAACCT GAAGTGGTTG
    GCGGATTTCC TTCGTGCCAA CCCCATTGAG ACCAGCGGTG CCCGCTGTGC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGGCACT
    GAAGATTACA GATCCAAATT AAGTGGTGAC TGCTTTGCAG ATTTGGCTTG CCCTGAGAAA
    TGTCGCTGTG AAGGGACCAC AGTGGACTGC TCCAATCAGA AACTCAACAA AATTCCTGAT
    CACATCCCAC AGTACACAGC AGAGTTGCGA CTCAATAACA ATGAATTTTC AGTCCTGGAA
    GCTACTGGGA TCTTTAAGAA GCTTCCTCAA CTGCGAAAAA TAAACCTGAG CAATAACAAG
    ATTACAGATA TTGAAGAAGG TGCATTTGAT GGAGCCTCTG GTGTCAATGA ACTATTGCTC
    ACTAGCAATC GTTTGGAAAC TGTTAGAGAC AAAATGTTCA AAGGACTGGA AAGTCTTAAA
    ACACTGATGC TGAGGAGTAA CCGTGTGAGC TGTGTGGGGA ACGACAGTTT CACAGGCCTG
    AGCTCTGTCC GTCTGCTCTC ACTATATGAC AACCAGATCA CCACCGTGGC ACCCGGCTCC
    TTCGATACCC TGCATTCACT CTCTACATTA AACCTCTTGG CCAATCCTTT CAACTGCAAC
    TGCCATCTTG CATGGCTTGG AGATTGGCTA AGGAAGAAAC GCATTGTGAC GGGAAACCCT
    CGCTGTCAGA AACCTTATTT CCTCAAAGAG ATTCCTATCC AGGATGTGGC AATTCAGGAT
    TTTACATGTG ATGATGGAAA TGATGACAAT AGCTGCTCTC CGCTGTCCCG CTGTCCTGCA
    GAATGTACTT GTCTAGACAC AGTTGTTCGC TGCAGCAACA AAGGCCTAAA AGCTTTGCCT
    AAAGGCATCC CAAAAGATGT AACTGAACTA TATTTGGATG GAAACCAGTT TACTCTTGTT
    CCTAAAGAGC TCTCCAACTA CAAACATTTA ACACTTATAG ATTTAAGTAA CAACAGAATC
    AGCACTCTTT CTAATCAGAG CTTCAGCAAC ATGACTCAGC TGCTCACCTT AATTCTTAGT
    TACAACCGCC TGAGGTGTAT CCCTGCACGG ACTTTTGATG GGTTGAAATC ACTTAGGTTG
    CTGTCTTTAC ATGGCAATGA TATTTCTGTG GTTCCTGAAG GAGCCTTTAA TGATCTTTCA
    GCGTTATCAC ACCTGGCTAT TGGAGCAAAT CCTCTTTATT GTGATTGTAA CATGCAATGG
    CTGTCTGACT GGGTAAAATC AGAATACAAA GAACCTGGTA TTGCACGATG TGCTGGCCCT
    GGAGAAATGG CAGATAAACT TCTACTTACA ACTCCATCTA AAAAATTTAC TTGCCAAGGG
    CCCGTGGATG TCAATATTCT TGCTAAGTGT AACCCCTGCT TATCAAATCC ATGTAAAAAT
    GATGGAACCT GCAATAATGA TCCAGTTGAC TTCTATAGAT GTACTTGCCC ATATGGTTTC
    AAGGGTCAAG ACTGTGATAT TCCCATTCAT GCCTGCATTA GTAACCCTTG CAACCATGGT
    GGAACTTGTC ATTTGAAAGA AGGAGAAAAA GATGGTTTCT GGTGCACTTG TGCAGATGGA
    TTTGAAGGAG AAAATTGTGA AATAAATGTT GATGACTGTG AAGACAATGA CTGTGAAAAT
    AACTCTACTT GTGTGGATGG AATTAATAAT TATACTTGCC TTTGTCCACC TGAATATACA
    GGTGAGCTCT GTGAGGAGAA ACTAGATTTC TGTGCTCAAA ACCTGAACCC TTGCCAGCAC
    GACTCAAAGT GTATCTTGAC TCCCAAAGGT TACAAGTGTG ATTGCACACC TGGATATGTA
    GGTGAACACT GCGATATTGA CTTCGATGAC TGCCAGGACA ATAAATGTAA AAACGGAGCA
    CAGTGTACGG ATGCAGTTAA CGGGTATACT TGTATTTGCC CAGAGGGATA CAGTGGCTTG
    TTTTGTGAGT TTTCGCCACC AATGGTTTTA CCTCGCACCA GCCCTTGTGA TAATTATGAA
    TGCCAAAATG GAGCCCAGTG TATTGTAAAG GAGAGTGAAC CAATCTGCCA GTGTTTATCA
    GGCTACCAGG GTGAGAAATG TGAAAAGCTG ATCAGTATAA ACTTTGTCAA CAAAGAATCC
    TATCTACAAA TCCCTTCAGC TAAGATACAC TCCCAAACCA ATATCACTCT TCAGATTGCC
    ACAGACGAAG ACAGTGGGAT CCTGCTCTAC AAAGGCGATA AGGATCATAT AGCAGTAGAG
    CTGTACCGTG GTAGAGTGAG GGTCAGTTAT GACACAGGAT CTTATCCAGC CTCTGCTATT
    TACAGTGTGG AAACTATTAA TGATGGCAAT TTCCACATTG TGGAGCTGCT TGCCATGGAT
    CAGATTCTGT CTTTGTCTAT TGATGGAGGA AGCCCCAAGA TAATTACCAA TTTGTCCAAG
    CAGTCCACTT TGAATTTTGA TTCTCCACTG TATGTCGGAG GCATGCCTGT GAAAAATAAC
    ATTGCAGCTC TACGTCAGTC TCCAGGACAG AATGGCACAA GCTTCCATGG CTGCATCCGT
    AATCTGTATA TCAACAGCGA ACTCCAGGAC TTCAGAAATG TGCCACTGCA AGTGGGAATT
    CTGCCAGGTT GCGAGCCTTG TCACAAGAAA GTTTGTGTGC ATGGAACATG CCATGCTACC
    AGCCAGTCAA GCTTTACCTG TGAGTGTGAA GGAGGATGGA CTGGACCCCT CTGTGATCAA
    CAAACTAATG ACCCGTGTCT CGGAAATAAA TGTGTGCATG GTACCTGCTT GCCGATCAAT
    GCATTTTCAT ACAGTTGTAA ATGCCTGCAG GGACATGGGG GAGTCCTCTG TGATGAAGAG
    GAAATGCTGT TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA
    GGACTTGGGA AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA
    GAAATCTCTT GTCGAGGGGA ACGAATCCGA GATTACTACC AAAAGCAGCA AGGGTATGCT
    GCGTGCCAGA CGACCAAGAA GGTATCGAGA CTAGAATGTA AAGGAGGATG TTCAACCGGG
    CAGTGCTGTG GACCACTAAG GAGCAAGAGA CGGAAATACT CTTTTGAATG CACTGATGGG
    TCGTCATTTG TGGACGAGAT TGAAAAAGTG GTGAAGTGTG GCTGTACAAA TTGTCCCTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015141202.1
    CDS1262..5824
    Translation

    Target ORF information:

    RefSeq Version XM_015285716.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit homolog 2 (Drosophila) (SLIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285716.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACTGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTAC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCA
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCAACCT GAAGTGGTTG
    GCGGATTTCC TTCGTGCCAA CCCCATTGAG ACCAGCGGTG CCCGCTGTGC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGGCACT
    GAAGATTACA GATCCAAATT AAGTGGTGAC TGCTTTGCAG ATTTGGCTTG CCCTGAGAAA
    TGTCGCTGTG AAGGGACCAC AGTGGACTGC TCCAATCAGA AACTCAACAA AATTCCTGAT
    CACATCCCAC AGTACACAGC AGAGTTGCGA CTCAATAACA ATGAATTTTC AGTCCTGGAA
    GCTACTGGGA TCTTTAAGAA GCTTCCTCAA CTGCGAAAAA TAAACCTGAG CAATAACAAG
    ATTACAGATA TTGAAGAAGG TGCATTTGAT GGAGCCTCTG GTGTCAATGA ACTATTGCTC
    ACTAGCAATC GTTTGGAAAC TGTTAGAGAC AAAATGTTCA AAGGACTGGA AAGTCTTAAA
    ACACTGATGC TGAGGAGTAA CCGTGTGAGC TGTGTGGGGA ACGACAGTTT CACAGGCCTG
    AGCTCTGTCC GTCTGCTCTC ACTATATGAC AACCAGATCA CCACCGTGGC ACCCGGCTCC
    TTCGATACCC TGCATTCACT CTCTACATTA AACCTCTTGG CCAATCCTTT CAACTGCAAC
    TGCCATCTTG CATGGCTTGG AGATTGGCTA AGGAAGAAAC GCATTGTGAC GGGAAACCCT
    CGCTGTCAGA AACCTTATTT CCTCAAAGAG ATTCCTATCC AGGATGTGGC AATTCAGGAT
    TTTACATGTG ATGATGGAAA TGATGACAAT AGCTGCTCTC CGCTGTCCCG CTGTCCTGCA
    GAATGTACTT GTCTAGACAC AGTTGTTCGC TGCAGCAACA AAGGCCTAAA AGCTTTGCCT
    AAAGGCATCC CAAAAGATGT AACTGAACTA TATTTGGATG GAAACCAGTT TACTCTTGTT
    CCTAAAGAGC TCTCCAACTA CAAACATTTA ACACTTATAG ATTTAAGTAA CAACAGAATC
    AGCACTCTTT CTAATCAGAG CTTCAGCAAC ATGACTCAGC TGCTCACCTT AATTCTTAGT
    TACAACCGCC TGAGGTGTAT CCCTGCACGG ACTTTTGATG GGTTGAAATC ACTTAGGTTG
    CTGTCTTTAC ATGGCAATGA TATTTCTGTG GTTCCTGAAG GAGCCTTTAA TGATCTTTCA
    GCGTTATCAC ACCTGGCTAT TGGAGCAAAT CCTCTTTATT GTGATTGTAA CATGCAATGG
    CTGTCTGACT GGGTAAAATC AGAATACAAA GAACCTGGTA TTGCACGATG TGCTGGCCCT
    GGAGAAATGG CAGATAAACT TCTACTTACA ACTCCATCTA AAAAATTTAC TTGCCAAGGG
    CCCGTGGATG TCAATATTCT TGCTAAGTGT AACCCCTGCT TATCAAATCC ATGTAAAAAT
    GATGGAACCT GCAATAATGA TCCAGTTGAC TTCTATAGAT GTACTTGCCC ATATGGTTTC
    AAGGGTCAAG ACTGTGATAT TCCCATTCAT GCCTGCATTA GTAACCCTTG CAACCATGGT
    GGAACTTGTC ATTTGAAAGA AGGAGAAAAA GATGGTTTCT GGTGCACTTG TGCAGATGGA
    TTTGAAGGAG AAAATTGTGA AATAAATGTT GATGACTGTG AAGACAATGA CTGTGAAAAT
    AACTCTACTT GTGTGGATGG AATTAATAAT TATACTTGCC TTTGTCCACC TGAATATACA
    GGTGAGCTCT GTGAGGAGAA ACTAGATTTC TGTGCTCAAA ACCTGAACCC TTGCCAGCAC
    GACTCAAAGT GTATCTTGAC TCCCAAAGGT TACAAGTGTG ATTGCACACC TGGATATGTA
    GGTGAACACT GCGATATTGA CTTCGATGAC TGCCAGGACA ATAAATGTAA AAACGGAGCA
    CAGTGTACGG ATGCAGTTAA CGGGTATACT TGTATTTGCC CAGAGGGATA CAGTGGCTTG
    TTTTGTGAGT TTTCGCCACC AATGGTTTTA CCTCGCACCA GCCCTTGTGA TAATTATGAA
    TGCCAAAATG GAGCCCAGTG TATTGTAAAG GAGAGTGAAC CAATCTGCCA GTGTTTATCA
    GGCTACCAGG GTGAGAAATG TGAAAAGCTG ATCAGTATAA ACTTTGTCAA CAAAGAATCC
    TATCTACAAA TCCCTTCAGC TAAGATACAC TCCCAAACCA ATATCACTCT TCAGATTGCC
    ACAGACGAAG ACAGTGGGAT CCTGCTCTAC AAAGGCGATA AGGATCATAT AGCAGTAGAG
    CTGTACCGTG GTAGAGTGAG GGTCAGTTAT GACACAGGAT CTTATCCAGC CTCTGCTATT
    TACAGTGTGG AAACTATTAA TGATGGCAAT TTCCACATTG TGGAGCTGCT TGCCATGGAT
    CAGATTCTGT CTTTGTCTAT TGATGGAGGA AGCCCCAAGA TAATTACCAA TTTGTCCAAG
    CAGTCCACTT TGAATTTTGA TTCTCCACTG TATGTCGGAG GCATGCCTGT GAAAAATAAC
    ATTGCAGCTC TACGTCAGTC TCCAGGACAG AATGGCACAA GCTTCCATGG CTGCATCCGT
    AATCTGTATA TCAACAGCGA ACTCCAGGAC TTCAGAAATG TGCCACTGCA AGTGGGAATT
    CTGCCAGGTT GCGAGCCTTG TCACAAGAAA GTTTGTGTGC ATGGAACATG CCATGCTACC
    AGCCAGTCAA GCTTTACCTG TGAGTGTGAA GGAGGATGGA CTGGACCCCT CTGTGATCAA
    CAAACTAATG ACCCGTGTCT CGGAAATAAA TGTGTGCATG GTACCTGCTT GCCGATCAAT
    GCATTTTCAT ACAGTTGTAA ATGCCTGCAG GGACATGGGG GAGTCCTCTG TGATGAAGAG
    GAAATGCTGT TTAACCCCTG CCAATCCATC AGGTGTAAAC ATGGCAAATG CAGGCTTTCA
    GGACTTGGGA AACCATATTG CGAATGCAGC AGCGGATACA CGGGGGACAG CTGTGATAAA
    GAAATCTCTT GTCGAGGGGA ACGAATCCGA GATTACTACC AAAAGCAGCA AGGGTATGCT
    GCGTGCCAGA CGACCAAGAA GGTATCGAGA CTAGAATGTA AAGGAGGATG TTCAACCGGG
    CAGTGCTGTG GACCACTAAG GAGCAAGAGA CGGAAATACT CTTTTGAATG CACTGATGGG
    TCGTCATTTG TGGACGAGAT TGAAAAAGTG GTGAAGTGTG GCTGTACAAA TTGTCCCTCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa23967
    Clone ID Related Accession (Same CDS sequence) NM_001267075.1
    Accession Version NM_001267075.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4587bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-12-24
    Organism Gallus gallus(chicken)
    Product slit homolog 2 protein precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN03003818.1. On or before May 31, 2012 this sequence version replaced XM_001232065.2, XM_001232040.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SRS000289, SRS213961 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACTGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTAC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGGTGAG CTCTGTGAGG AGAAACTAGA TTTCTGTGCT
    CAAAACCTGA ACCCTTGCCA GCACGACTCA AAGTGTATCT TGACTCCCAA AGGTTACAAG
    TGTGATTGCA CACCTGGATA TGTAGGTGAA CACTGCGATA TTGACTTCGA TGACTGCCAG
    GACAATAAAT GTAAAAACGG AGCACAGTGT ACGGATGCAG TTAACGGGTA TACTTGTATT
    TGCCCAGAGG GATACAGTGG CTTGTTTTGT GAGTTTTCGC CACCAATGGT TTTACCTCGC
    ACCAGCCCTT GTGATAATTA TGAATGCCAA AATGGAGCCC AGTGTATTGT AAAGGAGAGT
    GAACCAATCT GCCAGTGTTT ATCAGGCTAC CAGGGTGAGA AATGTGAAAA GCTGATCAGT
    ATAAACTTTG TCAACAAAGA ATCCTATCTA CAAATCCCTT CAGCTAAGAT ACACTCCCAA
    ACCAATATCA CTCTTCAGAT TGCCACAGAC GAAGACAGTG GGATCCTGCT CTACAAAGGC
    GATAAGGATC ATATAGCAGT AGAGCTGTAC CGTGGTAGAG TGAGGGTCAG TTATGACACA
    GGATCTTATC CAGCCTCTGC TATTTACAGT GTGGAAACTA TTAATGATGG CAATTTCCAC
    ATTGTGGAGC TGCTTGCCAT GGATCAGATT CTGTCTTTGT CTATTGATGG AGGAAGCCCC
    AAGATAATTA CCAATTTGTC CAAGCAGTCC ACTTTGAATT TTGATTCTCC ACTGTATGTC
    GGAGGCATGC CTGTGAAAAA TAACATTGCA GCTCTACGTC AGTCTCCAGG ACAGAATGGC
    ACAAGCTTCC ATGGCTGCAT CCGTAATCTG TATATCAACA GCGAACTCCA GGACTTCAGA
    AATGTGCCAC TGCAAGTGGG AATTCTGCCA GGTTGCGAGC CTTGTCACAA GAAAGTTTGT
    GTGCATGGAA CATGCCATGC TACCAGCCAG TCAAGCTTTA CCTGTGAGTG TGAAGGAGGA
    TGGACTGGAC CCCTCTGTGA TCAACAAACT AATGACCCGT GTCTCGGAAA TAAATGTGTG
    CATGGTACCT GCTTGCCGAT CAATGCATTT TCATACAGTT GTAAATGCCT GCAGGGACAT
    GGGGGAGTCC TCTGTGATGA AGAGGAAATG CTGTTTAACC CCTGCCAATC CATCAGGTGT
    AAACATGGCA AATGCAGGCT TTCAGGACTT GGGAAACCAT ATTGCGAATG CAGCAGCGGA
    TACACGGGGG ACAGCTGTGA TAAAGAAATC TCTTGTCGAG GGGAACGAAT CCGAGATTAC
    TACCAAAAGC AGCAAGGGTA TGCTGCGTGC CAGACGACCA AGAAGGTATC GAGACTAGAA
    TGTAAAGGAG GATGTTCAAC CGGGCAGTGC TGTGGACCAC TAAGGAGCAA GAGACGGAAA
    TACTCTTTTG AATGCACTGA TGGGTCGTCA TTTGTGGACG AGATTGAAAA AGTGGTGAAG
    TGTGGCTGTA CAAATTGTCC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001254004.1
    CDS360..4946
    Translation

    Target ORF information:

    RefSeq Version NM_001267075.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus slit homolog 2 (Drosophila) (SLIT2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001267075.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGATGTGCG CCTGGGGGAG GCTCCCCCTG GCCCTGGGGC TGCTGCTGGT GCTGGCGGGC 
    GAGGCGGCGC CGCAGCCGTG CCCGGCGCAG TGCTCCTGCT CAGGAAGCAC GGTGGACTGT
    CACGGGCTGG CGCTGCGCGG CGTCCCGAGG AACATCCCCC GCAACACTGA GCGGCTGGAC
    CTTAATGGAA ATAACATCAC CAGAATCACC AAGACCGACT TTGCTGGTCT AAGGCACCTT
    CGAGTTCTTC AGCTCATGGA GAACAAGATT AGCACTATTG AGAGAGGAGC ATTCCAGGAT
    TTAAAAGAAC TGGAGAGGCT GCGCCTAAAC AGAAATAACC TCCAGTTGCT TTCTGAACTG
    CTCTTTCTGG GGACGCCGAA GTTATACAGG CTTGATCTTA GTGAAAATCA GATTCAAGCC
    ATACCCAGGA AGGCATTTCG TGGAGCAGTA GACATAAAAA ATCTGCAACT GGATTACAAC
    CAGATCAGCT GTATTGAAGA TGGGGCATTT AGGGCTCTAC GCGACCTGGA AGTGCTCACT
    CTCAACAACA ATAACATTAC TCGACTGTCC GTCGCAAGTT TCAATCATAT GCCCAAACTC
    AGAACTTTTC GCCTGCACTC CAACAACCTC TACTGTGACT GCCACCTGGC CTGGCTGTCG
    GACTGGCTGC GGCAGCGGCC ACGTGTAGGC CTCTACACTC AGTGCATGGG CCCAGCACAC
    CTGCGGGGCC ATAACGTGGC TGAGGTCCAG AAGCGGGAGT TCGTCTGCAG TGGTCACCAA
    TCATTTATGG CTCCATCCTG CAGTGTCTTG CATTGTCCTG CTGCATGCAC CTGTAGTAAC
    AACATTGTGG ACTGTCGTGG GAAAGGCCTT ACTGAAATTC CAACAAATCT TCCAGAAACC
    ATTACTGAAA TACGGTTAGA ACAAAATTCA ATCAAAGTCA TACCTCCTGG AGCTTTCTCA
    CCCTATAAAA AGCTTCGAAG AATTGACCTG AGCAATAACC AGATCTCTGA AGCAGCTCCA
    GATGCTTTCC AGGGCTTACG TTCTCTCAAT TCACTTGTCC TCTATGGCAA TAAAATTACA
    GAACTTCCAA AAGGCCTATT TGAAGGACTG TTTTCTCTGC AATTGCTATT ATTAAATGCC
    AACAAGATCA ATTGCCTGCG TGTTGATGCT TTTCAAGATC TGCACAACTT GAATCTCCTA
    TCTTTATATG ACAACAAGCT TCAGACCATT GCAAAAGGCA CCTTTTCACC TCTACGTGCA
    ATTCAGACCT TGCATTTGGC TCAGAACCCA TTTATCTGTG ACTGCCATCT GAAGTGGCTG
    GCGGATTATC TTCATACAAA CCCCATTGAG ACCAGTGGTG CCCGCTGCAC CAGCCCCCGC
    CGTCTGGCAA ACAAAAGGAT CGGCCAGATC AAAAGCAAGA AATTCCGCTG CTCAGCTAAA
    GAGCAGTATT TCATTCCAGG CACTGAAGAT TACAGATCCA AATTAAGTGG TGACTGCTTT
    GCAGATTTGG CTTGCCCTGA GAAATGTCGC TGTGAAGGGA CCACAGTGGA CTGCTCCAAT
    CAGAAACTCA ACAAAATTCC TGATCACATC CCACAGTACA CAGCAGAGTT GCGACTCAAT
    AACAATGAAT TTTCAGTCCT GGAAGCTACT GGGATCTTTA AGAAGCTTCC TCAACTGCGA
    AAAATAAACC TGAGCAATAA CAAGATTACA GATATTGAAG AAGGTGCATT TGATGGAGCC
    TCTGGTGTCA ATGAACTATT GCTCACTAGC AATCGTTTGG AAACTGTTAG AGACAAAATG
    TTCAAAGGAC TGGAAAGTCT TAAAACACTG ATGCTGAGGA GTAACCGTGT GAGCTGTGTG
    GGGAACGACA GTTTCACAGG CCTGAGCTCT GTCCGTCTGC TCTCACTATA TGACAACCAG
    ATCACCACCG TGGCACCCGG CTCCTTCGAT ACCCTGCATT CACTCTCTAC ATTAAACCTC
    TTGGCCAATC CTTTCAACTG CAACTGCCAT CTTGCATGGC TTGGAGATTG GCTAAGGAAG
    AAACGCATTG TGACGGGAAA CCCTCGCTGT CAGAAACCTT ATTTCCTCAA AGAGATTCCT
    ATCCAGGATG TGGCAATTCA GGATTTTACA TGTGATGATG GAAATGATGA CAATAGCTGC
    TCTCCGCTGT CCCGCTGTCC TGCAGAATGT ACTTGTCTAG ACACAGTTGT TCGCTGCAGC
    AACAAAGGCC TAAAAGCTTT GCCTAAAGGC ATCCCAAAAG ATGTAACTGA ACTATATTTG
    GATGGAAACC AGTTTACTCT TGTTCCTAAA GAGCTCTCCA ACTACAAACA TTTAACACTT
    ATAGATTTAA GTAACAACAG AATCAGCACT CTTTCTAATC AGAGCTTCAG CAACATGACT
    CAGCTGCTCA CCTTAATTCT TAGTTACAAC CGCCTGAGGT GTATCCCTGC ACGGACTTTT
    GATGGGTTGA AATCACTTAG GTTGCTGTCT TTACATGGCA ATGATATTTC TGTGGTTCCT
    GAAGGAGCCT TTAATGATCT TTCAGCGTTA TCACACCTGG CTATTGGAGC AAATCCTCTT
    TATTGTGATT GTAACATGCA ATGGCTGTCT GACTGGGTAA AATCAGAATA CAAAGAACCT
    GGTATTGCAC GATGTGCTGG CCCTGGAGAA ATGGCAGATA AACTTCTACT TACAACTCCA
    TCTAAAAAAT TTACTTGCCA AGGGCCCGTG GATGTCAATA TTCTTGCTAA GTGTAACCCC
    TGCTTATCAA ATCCATGTAA AAATGATGGA ACCTGCAATA ATGATCCAGT TGACTTCTAT
    AGATGTACTT GCCCATATGG TTTCAAGGGT CAAGACTGTG ATATTCCCAT TCATGCCTGC
    ATTAGTAACC CTTGCAACCA TGGTGGAACT TGTCATTTGA AAGAAGGAGA AAAAGATGGT
    TTCTGGTGCA CTTGTGCAGA TGGATTTGAA GGAGAAAATT GTGAAATAAA TGTTGATGAC
    TGTGAAGACA ATGACTGTGA AAATAACTCT ACTTGTGTGG ATGGAATTAA TAATTATACT
    TGCCTTTGTC CACCTGAATA TACAGGTGAG CTCTGTGAGG AGAAACTAGA TTTCTGTGCT
    CAAAACCTGA ACCCTTGCCA GCACGACTCA AAGTGTATCT TGACTCCCAA AGGTTACAAG
    TGTGATTGCA CACCTGGATA TGTAGGTGAA CACTGCGATA TTGACTTCGA TGACTGCCAG
    GACAATAAAT GTAAAAACGG AGCACAGTGT ACGGATGCAG TTAACGGGTA TACTTGTATT
    TGCCCAGAGG GATACAGTGG CTTGTTTTGT GAGTTTTCGC CACCAATGGT TTTACCTCGC
    ACCAGCCCTT GTGATAATTA TGAATGCCAA AATGGAGCCC AGTGTATTGT AAAGGAGAGT
    GAACCAATCT GCCAGTGTTT ATCAGGCTAC CAGGGTGAGA AATGTGAAAA GCTGATCAGT
    ATAAACTTTG TCAACAAAGA ATCCTATCTA CAAATCCCTT CAGCTAAGAT ACACTCCCAA
    ACCAATATCA CTCTTCAGAT TGCCACAGAC GAAGACAGTG GGATCCTGCT CTACAAAGGC
    GATAAGGATC ATATAGCAGT AGAGCTGTAC CGTGGTAGAG TGAGGGTCAG TTATGACACA
    GGATCTTATC CAGCCTCTGC TATTTACAGT GTGGAAACTA TTAATGATGG CAATTTCCAC
    ATTGTGGAGC TGCTTGCCAT GGATCAGATT CTGTCTTTGT CTATTGATGG AGGAAGCCCC
    AAGATAATTA CCAATTTGTC CAAGCAGTCC ACTTTGAATT TTGATTCTCC ACTGTATGTC
    GGAGGCATGC CTGTGAAAAA TAACATTGCA GCTCTACGTC AGTCTCCAGG ACAGAATGGC
    ACAAGCTTCC ATGGCTGCAT CCGTAATCTG TATATCAACA GCGAACTCCA GGACTTCAGA
    AATGTGCCAC TGCAAGTGGG AATTCTGCCA GGTTGCGAGC CTTGTCACAA GAAAGTTTGT
    GTGCATGGAA CATGCCATGC TACCAGCCAG TCAAGCTTTA CCTGTGAGTG TGAAGGAGGA
    TGGACTGGAC CCCTCTGTGA TCAACAAACT AATGACCCGT GTCTCGGAAA TAAATGTGTG
    CATGGTACCT GCTTGCCGAT CAATGCATTT TCATACAGTT GTAAATGCCT GCAGGGACAT
    GGGGGAGTCC TCTGTGATGA AGAGGAAATG CTGTTTAACC CCTGCCAATC CATCAGGTGT
    AAACATGGCA AATGCAGGCT TTCAGGACTT GGGAAACCAT ATTGCGAATG CAGCAGCGGA
    TACACGGGGG ACAGCTGTGA TAAAGAAATC TCTTGTCGAG GGGAACGAAT CCGAGATTAC
    TACCAAAAGC AGCAAGGGTA TGCTGCGTGC CAGACGACCA AGAAGGTATC GAGACTAGAA
    TGTAAAGGAG GATGTTCAAC CGGGCAGTGC TGTGGACCAC TAAGGAGCAA GAGACGGAAA
    TACTCTTTTG AATGCACTGA TGGGTCGTCA TTTGTGGACG AGATTGAAAA AGTGGTGAAG
    TGTGGCTGTA CAAATTGTCC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.