Slit2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006503815.3	XP_006503878.1	slit homolog 2 protein isoform X3
XM_006503816.4	XP_006503879.1	slit homolog 2 protein isoform X4
NM_001291228.2	NP_001278157.1	slit homolog 2 protein isoform b precursor
XM_006503814.3	XP_006503877.1	slit homolog 2 protein isoform X2
XM_006503813.4	XP_006503876.1	slit homolog 2 protein isoform X1
XM_006503814.4	XP_006503877.1	slit homolog 2 protein isoform X2
XM_006503813.3	XP_006503876.1	slit homolog 2 protein isoform X1
NM_178804.5	NP_848919.3	slit homolog 2 protein isoform c precursor
XM_006503816.3	XP_006503879.1	slit homolog 2 protein isoform X4
NM_001291227.2	NP_001278156.1	slit homolog 2 protein isoform a precursor
XM_006503815.4	XP_006503878.1	slit homolog 2 protein isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu34990	XM_006503813.4 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu34991	XM_006503814.4 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu34992	XM_006503815.4 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu34993	XM_006503816.4 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu17705	NM_178804.5 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$895.30 $1279.00
OMu17713	NM_001291227.2 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu17803	NM_001291228.2 Latest version!	Mus musculus slit guidance ligand 2 (Slit2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu34991	XM_006503814.3	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu34990	XM_006503813.3	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu34993	XM_006503816.3	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu34992	XM_006503815.3	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
Hide Old Accession Versions >>

Slit2 ( XM_006503813.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503876.1 Mus musculus slit guidance ligand 2 (Slit2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34990
Clone ID Related Accession (Same CDS sequence)	XM_006503813.4 , XM_006503813.3
Accession Version	XM_006503813.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503813.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGCT GCTAATCTGA ATGAGGTGGA AAAAGGTGAA 
CTGTGTGAGG AAAAGCTGGA CTTCTGTGCA CAAGACTTGA ATCCCTGCCA GCATGACTCC 
AAGTGCATCC TGACTCCAAA GGGATTCAAG TGTGACTGCA CTCCAGGATA CATTGGTGAG 
CACTGTGACA TTGACTTTGA TGACTGCCAA GATAACAAGT GTAAAAACGG TGCTCACTGC 
ACAGATGCCG TGAACGGATA CACGTGCGTC TGTCCTGAAG GCTACAGTGG CTTGTTCTGT 
GAGTTTTCTC CACCCATGGT CCTCCCTCGC ACCAGCCCCT GTGATAATTT TGATTGCCAG 
AATGGAGCCC AGTGTATCAT CAGGATAAAT GAACCAATAT GCCAGTGTTT GCCTGGCTAC 
CTGGGAGAGA AGTGTGAGAA ATTGGTCAGT GTGAATTTTG TAAACAAAGA GTCCTATCTT 
CAGATTCCTT CAGCCAAGGT TCGGCCTCAG ACAAACATCA CACTTCAGAT TGCCACAGAT 
GAAGACAGCG GCATCCTCTT GTATAAAGGT GACAAAGACC ACATTGCCGT GGAACTCTAT 
AGAGGGCGAG TTCGAGCCAG CTATGACACC GGCTCTCATC CGGCTTCTGC CATTTACAGT 
GTGGAGACAA TCAATGATGG AAACTTCCAC ATTGTGGAGC TACTGACCCT GGATTCCAGT 
CTTTCCCTCT CTGTGGATGG AGGAAGCCCT AAAGTCATCA CCAATTTGTC AAAACAATCT 
ACTCTGAATT TCGACTCTCC ACTCTATGTA GGAGGCATGC CTGGGAAAAA TAACGTGGCA 
TCCCTGCGCC AGGCCCCTGG GCAAAATGGC ACCAGCTTCC ATGGCTGTAT CCGGAACCTT 
TACATTAACA GTGAGCTGCA GGACTTCCGG AAAATGCCTA TGCAAACCGG AATTCTGCCT 
GGCTGTGAAC CATGCCACAA GAAAGTATGT GCCCATGGCA TGTGCCAGCC CAGCAGCCAA 
TCAGGCTTCA CCTGTGAATG TGAGGAAGGG TGGATGGGGC CCCTCTGTGA CCAGAGAACC 
AATGATCCCT GCCTCGGAAA CAAATGTGTG CATGGGACCT GCCTGCCCAT CAATGCCTTC 
TCCTATAGTT GCAAGTGCCT GGAGGGCCAT GGCGGTGTCC TCTGTGATGA AGAAGAAGAT 
CTCTTTAACC CCTGCCAGAT GATCAAGTGC AAGCATGGGA AGTGCAGGCT TTCTGGAGTG 
GGCCAGCCCT ATTGTGAATG CAACAGTGGA TTCACCGGGG ACAGCTGTGA TAGAGAAATT 
TCTTGTCGAG GGGAACGGAT AAGGGACTAT TACCAGAAGC AGCAGGGTTA CGCTGCCTGT 
CAAACAACTA AGAAAGTATC TCGCTTGGAA TGCAGAGGCG GGTGCGCTGG AGGCCAGTGC 
TGTGGACCTC TGAGAAGCAA GAGGCGGAAA TACTCTTTCG AATGCACAGA TGGCTCCTCA 
TTTGTGGACG AGGTTGAGAA AGTGGTGAAG TGCGGCTGCG CGAGATGTGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503876.1
CDS	3750..8366
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503813.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503813.4

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGCT GCTAATCTGA ATGAGGTGGA AAAAGGTGAA 
CTGTGTGAGG AAAAGCTGGA CTTCTGTGCA CAAGACTTGA ATCCCTGCCA GCATGACTCC 
AAGTGCATCC TGACTCCAAA GGGATTCAAG TGTGACTGCA CTCCAGGATA CATTGGTGAG 
CACTGTGACA TTGACTTTGA TGACTGCCAA GATAACAAGT GTAAAAACGG TGCTCACTGC 
ACAGATGCCG TGAACGGATA CACGTGCGTC TGTCCTGAAG GCTACAGTGG CTTGTTCTGT 
GAGTTTTCTC CACCCATGGT CCTCCCTCGC ACCAGCCCCT GTGATAATTT TGATTGCCAG 
AATGGAGCCC AGTGTATCAT CAGGATAAAT GAACCAATAT GCCAGTGTTT GCCTGGCTAC 
CTGGGAGAGA AGTGTGAGAA ATTGGTCAGT GTGAATTTTG TAAACAAAGA GTCCTATCTT 
CAGATTCCTT CAGCCAAGGT TCGGCCTCAG ACAAACATCA CACTTCAGAT TGCCACAGAT 
GAAGACAGCG GCATCCTCTT GTATAAAGGT GACAAAGACC ACATTGCCGT GGAACTCTAT 
AGAGGGCGAG TTCGAGCCAG CTATGACACC GGCTCTCATC CGGCTTCTGC CATTTACAGT 
GTGGAGACAA TCAATGATGG AAACTTCCAC ATTGTGGAGC TACTGACCCT GGATTCCAGT 
CTTTCCCTCT CTGTGGATGG AGGAAGCCCT AAAGTCATCA CCAATTTGTC AAAACAATCT 
ACTCTGAATT TCGACTCTCC ACTCTATGTA GGAGGCATGC CTGGGAAAAA TAACGTGGCA 
TCCCTGCGCC AGGCCCCTGG GCAAAATGGC ACCAGCTTCC ATGGCTGTAT CCGGAACCTT 
TACATTAACA GTGAGCTGCA GGACTTCCGG AAAATGCCTA TGCAAACCGG AATTCTGCCT 
GGCTGTGAAC CATGCCACAA GAAAGTATGT GCCCATGGCA TGTGCCAGCC CAGCAGCCAA 
TCAGGCTTCA CCTGTGAATG TGAGGAAGGG TGGATGGGGC CCCTCTGTGA CCAGAGAACC 
AATGATCCCT GCCTCGGAAA CAAATGTGTG CATGGGACCT GCCTGCCCAT CAATGCCTTC 
TCCTATAGTT GCAAGTGCCT GGAGGGCCAT GGCGGTGTCC TCTGTGATGA AGAAGAAGAT 
CTCTTTAACC CCTGCCAGAT GATCAAGTGC AAGCATGGGA AGTGCAGGCT TTCTGGAGTG 
GGCCAGCCCT ATTGTGAATG CAACAGTGGA TTCACCGGGG ACAGCTGTGA TAGAGAAATT 
TCTTGTCGAG GGGAACGGAT AAGGGACTAT TACCAGAAGC AGCAGGGTTA CGCTGCCTGT 
CAAACAACTA AGAAAGTATC TCGCTTGGAA TGCAGAGGCG GGTGCGCTGG AGGCCAGTGC 
TGTGGACCTC TGAGAAGCAA GAGGCGGAAA TACTCTTTCG AATGCACAGA TGGCTCCTCA 
TTTGTGGACG AGGTTGAGAA AGTGGTGAAG TGCGGCTGCG CGAGATGTGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503814.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503877.1 Mus musculus slit guidance ligand 2 (Slit2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34991
Clone ID Related Accession (Same CDS sequence)	XM_006503814.3 , XM_006503814.4
Accession Version	XM_006503814.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503814.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG 
GCTTGTCCTG AGAAGTGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAGACTC 
AACAAAATCC CTGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAGTTACG TAAAATCAAC 
TTTAGCAACA ATAAGATCAC GGATATCGAG GAGGGTGCAT TTGAAGGCGC GTCTGGTGTG 
AATGAAATTC TTCTCACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA 
CTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT TGGGAACGAC 
AGTTTCATAG GACTCGGCTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA 
GTGGCACCAG GAGCATTTGA TTCTCTCCAT TCATTATCCA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGTCA CCTGGCATGG CTGGGAGAAT GGCTCAGAAG GAAAAGAATT 
GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCCTGA AGGAAATCCC AATCCAGGAT 
GTAGCCATTC AGGACTTCAC CTGTGATGAT GGAAATGATG ACAATAGTTG CTCTCCACTC 
TCCCGTTGTC CTTCTGAATG TACCTGCTTG GATACAGTGG TACGATGTAG CAACAAGGGC 
TTGAAGGTTT TGCCTAAAGG TATTCCAAAA GATGTCACAG AGCTGTATCT GGATGGGAAC 
CAGTTTACGC TGGTCCCGAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACC GAATAAGCAC CCTTTCCAAT CAAAGCTTCA GCAACATGAC CCAGCTTCTC 
ACCTTAATCC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGAACCTT TGATGGATTG 
AAGTCTCTTC GGTTACTGTC TTTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCC 
TTCAATGACT TGTCAGCCTT GTCACACTTA GCGATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCA 
CGCTGTGCCG GCCCTGGAGA AATGGCAGAT AAATTATTAC TCACTACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCCGT GGATATCACT ATTCAAGCCA AGTGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCCG TTGATTTTTA TCGATGTACC 
TGCCCATATG GATTCAAGGG TCAGGACTGT GATGTCCCCA TTCATGCTTG TATCAGTAAT 
CCATGTAAAC ATGGAGGAAC TTGTCACTTA AAGGAAGGAG AGAATGCTGG ATTCTGGTGC 
ACTTGTGCTG ATGGGTTTGA AGGAGAAAAC TGTGAAGTCA ATATTGATGA TTGTGAAGAT 
AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC ATGTCTTTGC 
CCACCGGAAT ACACAGCTGC TAATCTGAAT GAGGTGGAAA AAGGTGAACT GTGTGAGGAA 
AAGCTGGACT TCTGTGCACA AGACTTGAAT CCCTGCCAGC ATGACTCCAA GTGCATCCTG 
ACTCCAAAGG GATTCAAGTG TGACTGCACT CCAGGATACA TTGGTGAGCA CTGTGACATT 
GACTTTGATG ACTGCCAAGA TAACAAGTGT AAAAACGGTG CTCACTGCAC AGATGCCGTG 
AACGGATACA CGTGCGTCTG TCCTGAAGGC TACAGTGGCT TGTTCTGTGA GTTTTCTCCA 
CCCATGGTCC TCCCTCGCAC CAGCCCCTGT GATAATTTTG ATTGCCAGAA TGGAGCCCAG 
TGTATCATCA GGATAAATGA ACCAATATGC CAGTGTTTGC CTGGCTACCT GGGAGAGAAG 
TGTGAGAAAT TGGTCAGTGT GAATTTTGTA AACAAAGAGT CCTATCTTCA GATTCCTTCA 
GCCAAGGTTC GGCCTCAGAC AAACATCACA CTTCAGATTG CCACAGATGA AGACAGCGGC 
ATCCTCTTGT ATAAAGGTGA CAAAGACCAC ATTGCCGTGG AACTCTATAG AGGGCGAGTT 
CGAGCCAGCT ATGACACCGG CTCTCATCCG GCTTCTGCCA TTTACAGTGT GGAGACAATC 
AATGATGGAA ACTTCCACAT TGTGGAGCTA CTGACCCTGG ATTCCAGTCT TTCCCTCTCT 
GTGGATGGAG GAAGCCCTAA AGTCATCACC AATTTGTCAA AACAATCTAC TCTGAATTTC 
GACTCTCCAC TCTATGTAGG AGGCATGCCT GGGAAAAATA ACGTGGCATC CCTGCGCCAG 
GCCCCTGGGC AAAATGGCAC CAGCTTCCAT GGCTGTATCC GGAACCTTTA CATTAACAGT 
GAGCTGCAGG ACTTCCGGAA AATGCCTATG CAAACCGGAA TTCTGCCTGG CTGTGAACCA 
TGCCACAAGA AAGTATGTGC CCATGGCATG TGCCAGCCCA GCAGCCAATC AGGCTTCACC 
TGTGAATGTG AGGAAGGGTG GATGGGGCCC CTCTGTGACC AGAGAACCAA TGATCCCTGC 
CTCGGAAACA AATGTGTGCA TGGGACCTGC CTGCCCATCA ATGCCTTCTC CTATAGTTGC 
AAGTGCCTGG AGGGCCATGG CGGTGTCCTC TGTGATGAAG AAGAAGATCT CTTTAACCCC 
TGCCAGATGA TCAAGTGCAA GCATGGGAAG TGCAGGCTTT CTGGAGTGGG CCAGCCCTAT 
TGTGAATGCA ACAGTGGATT CACCGGGGAC AGCTGTGATA GAGAAATTTC TTGTCGAGGG 
GAACGGATAA GGGACTATTA CCAGAAGCAG CAGGGTTACG CTGCCTGTCA AACAACTAAG 
AAAGTATCTC GCTTGGAATG CAGAGGCGGG TGCGCTGGAG GCCAGTGCTG TGGACCTCTG 
AGAAGCAAGA GGCGGAAATA CTCTTTCGAA TGCACAGATG GCTCCTCATT TGTGGACGAG 
GTTGAGAAAG TGGTGAAGTG CGGCTGCGCG AGATGTGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503877.1
CDS	3750..8354
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503814.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503814.4

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG 
GCTTGTCCTG AGAAGTGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAGACTC 
AACAAAATCC CTGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAGTTACG TAAAATCAAC 
TTTAGCAACA ATAAGATCAC GGATATCGAG GAGGGTGCAT TTGAAGGCGC GTCTGGTGTG 
AATGAAATTC TTCTCACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA 
CTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT TGGGAACGAC 
AGTTTCATAG GACTCGGCTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA 
GTGGCACCAG GAGCATTTGA TTCTCTCCAT TCATTATCCA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGTCA CCTGGCATGG CTGGGAGAAT GGCTCAGAAG GAAAAGAATT 
GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCCTGA AGGAAATCCC AATCCAGGAT 
GTAGCCATTC AGGACTTCAC CTGTGATGAT GGAAATGATG ACAATAGTTG CTCTCCACTC 
TCCCGTTGTC CTTCTGAATG TACCTGCTTG GATACAGTGG TACGATGTAG CAACAAGGGC 
TTGAAGGTTT TGCCTAAAGG TATTCCAAAA GATGTCACAG AGCTGTATCT GGATGGGAAC 
CAGTTTACGC TGGTCCCGAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACC GAATAAGCAC CCTTTCCAAT CAAAGCTTCA GCAACATGAC CCAGCTTCTC 
ACCTTAATCC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGAACCTT TGATGGATTG 
AAGTCTCTTC GGTTACTGTC TTTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCC 
TTCAATGACT TGTCAGCCTT GTCACACTTA GCGATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCA 
CGCTGTGCCG GCCCTGGAGA AATGGCAGAT AAATTATTAC TCACTACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCCGT GGATATCACT ATTCAAGCCA AGTGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCCG TTGATTTTTA TCGATGTACC 
TGCCCATATG GATTCAAGGG TCAGGACTGT GATGTCCCCA TTCATGCTTG TATCAGTAAT 
CCATGTAAAC ATGGAGGAAC TTGTCACTTA AAGGAAGGAG AGAATGCTGG ATTCTGGTGC 
ACTTGTGCTG ATGGGTTTGA AGGAGAAAAC TGTGAAGTCA ATATTGATGA TTGTGAAGAT 
AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC ATGTCTTTGC 
CCACCGGAAT ACACAGCTGC TAATCTGAAT GAGGTGGAAA AAGGTGAACT GTGTGAGGAA 
AAGCTGGACT TCTGTGCACA AGACTTGAAT CCCTGCCAGC ATGACTCCAA GTGCATCCTG 
ACTCCAAAGG GATTCAAGTG TGACTGCACT CCAGGATACA TTGGTGAGCA CTGTGACATT 
GACTTTGATG ACTGCCAAGA TAACAAGTGT AAAAACGGTG CTCACTGCAC AGATGCCGTG 
AACGGATACA CGTGCGTCTG TCCTGAAGGC TACAGTGGCT TGTTCTGTGA GTTTTCTCCA 
CCCATGGTCC TCCCTCGCAC CAGCCCCTGT GATAATTTTG ATTGCCAGAA TGGAGCCCAG 
TGTATCATCA GGATAAATGA ACCAATATGC CAGTGTTTGC CTGGCTACCT GGGAGAGAAG 
TGTGAGAAAT TGGTCAGTGT GAATTTTGTA AACAAAGAGT CCTATCTTCA GATTCCTTCA 
GCCAAGGTTC GGCCTCAGAC AAACATCACA CTTCAGATTG CCACAGATGA AGACAGCGGC 
ATCCTCTTGT ATAAAGGTGA CAAAGACCAC ATTGCCGTGG AACTCTATAG AGGGCGAGTT 
CGAGCCAGCT ATGACACCGG CTCTCATCCG GCTTCTGCCA TTTACAGTGT GGAGACAATC 
AATGATGGAA ACTTCCACAT TGTGGAGCTA CTGACCCTGG ATTCCAGTCT TTCCCTCTCT 
GTGGATGGAG GAAGCCCTAA AGTCATCACC AATTTGTCAA AACAATCTAC TCTGAATTTC 
GACTCTCCAC TCTATGTAGG AGGCATGCCT GGGAAAAATA ACGTGGCATC CCTGCGCCAG 
GCCCCTGGGC AAAATGGCAC CAGCTTCCAT GGCTGTATCC GGAACCTTTA CATTAACAGT 
GAGCTGCAGG ACTTCCGGAA AATGCCTATG CAAACCGGAA TTCTGCCTGG CTGTGAACCA 
TGCCACAAGA AAGTATGTGC CCATGGCATG TGCCAGCCCA GCAGCCAATC AGGCTTCACC 
TGTGAATGTG AGGAAGGGTG GATGGGGCCC CTCTGTGACC AGAGAACCAA TGATCCCTGC 
CTCGGAAACA AATGTGTGCA TGGGACCTGC CTGCCCATCA ATGCCTTCTC CTATAGTTGC 
AAGTGCCTGG AGGGCCATGG CGGTGTCCTC TGTGATGAAG AAGAAGATCT CTTTAACCCC 
TGCCAGATGA TCAAGTGCAA GCATGGGAAG TGCAGGCTTT CTGGAGTGGG CCAGCCCTAT 
TGTGAATGCA ACAGTGGATT CACCGGGGAC AGCTGTGATA GAGAAATTTC TTGTCGAGGG 
GAACGGATAA GGGACTATTA CCAGAAGCAG CAGGGTTACG CTGCCTGTCA AACAACTAAG 
AAAGTATCTC GCTTGGAATG CAGAGGCGGG TGCGCTGGAG GCCAGTGCTG TGGACCTCTG 
AGAAGCAAGA GGCGGAAATA CTCTTTCGAA TGCACAGATG GCTCCTCATT TGTGGACGAG 
GTTGAGAAAG TGGTGAAGTG CGGCTGCGCG AGATGTGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503815.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503878.1 Mus musculus slit guidance ligand 2 (Slit2), transcript variant X3, mRNA.

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CloneID	OMu34992
Clone ID Related Accession (Same CDS sequence)	XM_006503815.3 , XM_006503815.4
Accession Version	XM_006503815.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503815.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAGAAGT GTCGCTGTGA AGGGACCACA 
GTAGACTGCT CCAATCAAAG ACTCAACAAA ATCCCTGACC ATATTCCCCA GTACACAGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA 
CTTCCTCAGT TACGTAAAAT CAACTTTAGC AACAATAAGA TCACGGATAT CGAGGAGGGT 
GCATTTGAAG GCGCGTCTGG TGTGAATGAA ATTCTTCTCA CCAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA AGGACTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT 
CGAATAAGCT GTGTTGGGAA CGACAGTTTC ATAGGACTCG GCTCTGTGCG TCTGCTCTCT 
TTATATGACA ATCAAATTAC CACAGTGGCA CCAGGAGCAT TTGATTCTCT CCATTCATTA 
TCCACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTGGGA 
GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC 
CTGAAGGAAA TCCCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGATGGAAAT 
GATGACAATA GTTGCTCTCC ACTCTCCCGT TGTCCTTCTG AATGTACCTG CTTGGATACA 
GTGGTACGAT GTAGCAACAA GGGCTTGAAG GTTTTGCCTA AAGGTATTCC AAAAGATGTC 
ACAGAGCTGT ATCTGGATGG GAACCAGTTT ACGCTGGTCC CGAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACCGAATAA GCACCCTTTC CAATCAAAGC 
TTCAGCAACA TGACCCAGCT TCTCACCTTA ATCCTCAGTT ACAACCGTCT GAGATGTATC 
CCTCCACGAA CCTTTGATGG ATTGAAGTCT CTTCGGTTAC TGTCTTTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCCTTCAAT GACTTGTCAG CCTTGTCACA CTTAGCGATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AACCTGGAAT TGCACGCTGT GCCGGCCCTG GAGAAATGGC AGATAAATTA 
TTACTCACTA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CCGTGGATAT CACTATTCAA 
GCCAAGTGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCCGTTGATT TTTATCGATG TACCTGCCCA TATGGATTCA AGGGTCAGGA CTGTGATGTC 
CCCATTCATG CTTGTATCAG TAATCCATGT AAACATGGAG GAACTTGTCA CTTAAAGGAA 
GGAGAGAATG CTGGATTCTG GTGCACTTGT GCTGATGGGT TTGAAGGAGA AAACTGTGAA 
GTCAATATTG ATGATTGTGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA 
ATTAACAACT ACACATGTCT TTGCCCACCG GAATACACAG GTGAACTGTG TGAGGAAAAG 
CTGGACTTCT GTGCACAAGA CTTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACT 
CCAAAGGGAT TCAAGTGTGA CTGCACTCCA GGATACATTG GTGAGCACTG TGACATTGAC 
TTTGATGACT GCCAAGATAA CAAGTGTAAA AACGGTGCTC ACTGCACAGA TGCCGTGAAC 
GGATACACGT GCGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC 
ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GCCAGAATGG AGCCCAGTGT 
ATCATCAGGA TAAATGAACC AATATGCCAG TGTTTGCCTG GCTACCTGGG AGAGAAGTGT 
GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC 
AAGGTTCGGC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC 
CTCTTGTATA AAGGTGACAA AGACCACATT GCCGTGGAAC TCTATAGAGG GCGAGTTCGA 
GCCAGCTATG ACACCGGCTC TCATCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GATGGAAACT TCCACATTGT GGAGCTACTG ACCCTGGATT CCAGTCTTTC CCTCTCTGTG 
GATGGAGGAA GCCCTAAAGT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC 
TCTCCACTCT ATGTAGGAGG CATGCCTGGG AAAAATAACG TGGCATCCCT GCGCCAGGCC 
CCTGGGCAAA ATGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAG 
CTGCAGGACT TCCGGAAAAT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC 
CACAAGAAAG TATGTGCCCA TGGCATGTGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT 
GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGCCTC 
GGAAACAAAT GTGTGCATGG GACCTGCCTG CCCATCAATG CCTTCTCCTA TAGTTGCAAG 
TGCCTGGAGG GCCATGGCGG TGTCCTCTGT GATGAAGAAG AAGATCTCTT TAACCCCTGC 
CAGATGATCA AGTGCAAGCA TGGGAAGTGC AGGCTTTCTG GAGTGGGCCA GCCCTATTGT 
GAATGCAACA GTGGATTCAC CGGGGACAGC TGTGATAGAG AAATTTCTTG TCGAGGGGAA 
CGGATAAGGG ACTATTACCA GAAGCAGCAG GGTTACGCTG CCTGTCAAAC AACTAAGAAA 
GTATCTCGCT TGGAATGCAG AGGCGGGTGC GCTGGAGGCC AGTGCTGTGG ACCTCTGAGA 
AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGCT CCTCATTTGT GGACGAGGTT 
GAGAAAGTGG TGAAGTGCGG CTGCGCGAGA TGTGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503878.1
CDS	3750..8351
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503815.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503815.4

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAGAAGT GTCGCTGTGA AGGGACCACA 
GTAGACTGCT CCAATCAAAG ACTCAACAAA ATCCCTGACC ATATTCCCCA GTACACAGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA 
CTTCCTCAGT TACGTAAAAT CAACTTTAGC AACAATAAGA TCACGGATAT CGAGGAGGGT 
GCATTTGAAG GCGCGTCTGG TGTGAATGAA ATTCTTCTCA CCAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA AGGACTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT 
CGAATAAGCT GTGTTGGGAA CGACAGTTTC ATAGGACTCG GCTCTGTGCG TCTGCTCTCT 
TTATATGACA ATCAAATTAC CACAGTGGCA CCAGGAGCAT TTGATTCTCT CCATTCATTA 
TCCACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTGGGA 
GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC 
CTGAAGGAAA TCCCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGATGGAAAT 
GATGACAATA GTTGCTCTCC ACTCTCCCGT TGTCCTTCTG AATGTACCTG CTTGGATACA 
GTGGTACGAT GTAGCAACAA GGGCTTGAAG GTTTTGCCTA AAGGTATTCC AAAAGATGTC 
ACAGAGCTGT ATCTGGATGG GAACCAGTTT ACGCTGGTCC CGAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACCGAATAA GCACCCTTTC CAATCAAAGC 
TTCAGCAACA TGACCCAGCT TCTCACCTTA ATCCTCAGTT ACAACCGTCT GAGATGTATC 
CCTCCACGAA CCTTTGATGG ATTGAAGTCT CTTCGGTTAC TGTCTTTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCCTTCAAT GACTTGTCAG CCTTGTCACA CTTAGCGATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AACCTGGAAT TGCACGCTGT GCCGGCCCTG GAGAAATGGC AGATAAATTA 
TTACTCACTA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CCGTGGATAT CACTATTCAA 
GCCAAGTGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCCGTTGATT TTTATCGATG TACCTGCCCA TATGGATTCA AGGGTCAGGA CTGTGATGTC 
CCCATTCATG CTTGTATCAG TAATCCATGT AAACATGGAG GAACTTGTCA CTTAAAGGAA 
GGAGAGAATG CTGGATTCTG GTGCACTTGT GCTGATGGGT TTGAAGGAGA AAACTGTGAA 
GTCAATATTG ATGATTGTGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA 
ATTAACAACT ACACATGTCT TTGCCCACCG GAATACACAG GTGAACTGTG TGAGGAAAAG 
CTGGACTTCT GTGCACAAGA CTTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACT 
CCAAAGGGAT TCAAGTGTGA CTGCACTCCA GGATACATTG GTGAGCACTG TGACATTGAC 
TTTGATGACT GCCAAGATAA CAAGTGTAAA AACGGTGCTC ACTGCACAGA TGCCGTGAAC 
GGATACACGT GCGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC 
ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GCCAGAATGG AGCCCAGTGT 
ATCATCAGGA TAAATGAACC AATATGCCAG TGTTTGCCTG GCTACCTGGG AGAGAAGTGT 
GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC 
AAGGTTCGGC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC 
CTCTTGTATA AAGGTGACAA AGACCACATT GCCGTGGAAC TCTATAGAGG GCGAGTTCGA 
GCCAGCTATG ACACCGGCTC TCATCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GATGGAAACT TCCACATTGT GGAGCTACTG ACCCTGGATT CCAGTCTTTC CCTCTCTGTG 
GATGGAGGAA GCCCTAAAGT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC 
TCTCCACTCT ATGTAGGAGG CATGCCTGGG AAAAATAACG TGGCATCCCT GCGCCAGGCC 
CCTGGGCAAA ATGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAG 
CTGCAGGACT TCCGGAAAAT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC 
CACAAGAAAG TATGTGCCCA TGGCATGTGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT 
GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGCCTC 
GGAAACAAAT GTGTGCATGG GACCTGCCTG CCCATCAATG CCTTCTCCTA TAGTTGCAAG 
TGCCTGGAGG GCCATGGCGG TGTCCTCTGT GATGAAGAAG AAGATCTCTT TAACCCCTGC 
CAGATGATCA AGTGCAAGCA TGGGAAGTGC AGGCTTTCTG GAGTGGGCCA GCCCTATTGT 
GAATGCAACA GTGGATTCAC CGGGGACAGC TGTGATAGAG AAATTTCTTG TCGAGGGGAA 
CGGATAAGGG ACTATTACCA GAAGCAGCAG GGTTACGCTG CCTGTCAAAC AACTAAGAAA 
GTATCTCGCT TGGAATGCAG AGGCGGGTGC GCTGGAGGCC AGTGCTGTGG ACCTCTGAGA 
AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGCT CCTCATTTGT GGACGAGGTT 
GAGAAAGTGG TGAAGTGCGG CTGCGCGAGA TGTGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503816.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503879.1 Mus musculus slit guidance ligand 2 (Slit2), transcript variant X4, mRNA.

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CloneID	OMu34993
Clone ID Related Accession (Same CDS sequence)	XM_006503816.4 , XM_006503816.3
Accession Version	XM_006503816.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006503816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGGT GAACTGTGTG AGGAAAAGCT GGACTTCTGT 
GCACAAGACT TGAATCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACTCC AAAGGGATTC 
AAGTGTGACT GCACTCCAGG ATACATTGGT GAGCACTGTG ACATTGACTT TGATGACTGC 
CAAGATAACA AGTGTAAAAA CGGTGCTCAC TGCACAGATG CCGTGAACGG ATACACGTGC 
GTCTGTCCTG AAGGCTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGCACCAGCC CCTGTGATAA TTTTGATTGC CAGAATGGAG CCCAGTGTAT CATCAGGATA 
AATGAACCAA TATGCCAGTG TTTGCCTGGC TACCTGGGAG AGAAGTGTGA GAAATTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCCTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACAAACA TCACACTTCA GATTGCCACA GATGAAGACA GCGGCATCCT CTTGTATAAA 
GGTGACAAAG ACCACATTGC CGTGGAACTC TATAGAGGGC GAGTTCGAGC CAGCTATGAC 
ACCGGCTCTC ATCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AGCTACTGAC CCTGGATTCC AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC 
CCTAAAGTCA TCACCAATTT GTCAAAACAA TCTACTCTGA ATTTCGACTC TCCACTCTAT 
GTAGGAGGCA TGCCTGGGAA AAATAACGTG GCATCCCTGC GCCAGGCCCC TGGGCAAAAT 
GGCACCAGCT TCCATGGCTG TATCCGGAAC CTTTACATTA ACAGTGAGCT GCAGGACTTC 
CGGAAAATGC CTATGCAAAC CGGAATTCTG CCTGGCTGTG AACCATGCCA CAAGAAAGTA 
TGTGCCCATG GCATGTGCCA GCCCAGCAGC CAATCAGGCT TCACCTGTGA ATGTGAGGAA 
GGGTGGATGG GGCCCCTCTG TGACCAGAGA ACCAATGATC CCTGCCTCGG AAACAAATGT 
GTGCATGGGA CCTGCCTGCC CATCAATGCC TTCTCCTATA GTTGCAAGTG CCTGGAGGGC 
CATGGCGGTG TCCTCTGTGA TGAAGAAGAA GATCTCTTTA ACCCCTGCCA GATGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCTGGA GTGGGCCAGC CCTATTGTGA ATGCAACAGT 
GGATTCACCG GGGACAGCTG TGATAGAGAA ATTTCTTGTC GAGGGGAACG GATAAGGGAC 
TATTACCAGA AGCAGCAGGG TTACGCTGCC TGTCAAACAA CTAAGAAAGT ATCTCGCTTG 
GAATGCAGAG GCGGGTGCGC TGGAGGCCAG TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG 
AAATACTCTT TCGAATGCAC AGATGGCTCC TCATTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GCGCGAGATG TGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503879.1
CDS	3750..8339
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503816.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503816.4

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGGT GAACTGTGTG AGGAAAAGCT GGACTTCTGT 
GCACAAGACT TGAATCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACTCC AAAGGGATTC 
AAGTGTGACT GCACTCCAGG ATACATTGGT GAGCACTGTG ACATTGACTT TGATGACTGC 
CAAGATAACA AGTGTAAAAA CGGTGCTCAC TGCACAGATG CCGTGAACGG ATACACGTGC 
GTCTGTCCTG AAGGCTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGCACCAGCC CCTGTGATAA TTTTGATTGC CAGAATGGAG CCCAGTGTAT CATCAGGATA 
AATGAACCAA TATGCCAGTG TTTGCCTGGC TACCTGGGAG AGAAGTGTGA GAAATTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCCTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACAAACA TCACACTTCA GATTGCCACA GATGAAGACA GCGGCATCCT CTTGTATAAA 
GGTGACAAAG ACCACATTGC CGTGGAACTC TATAGAGGGC GAGTTCGAGC CAGCTATGAC 
ACCGGCTCTC ATCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AGCTACTGAC CCTGGATTCC AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC 
CCTAAAGTCA TCACCAATTT GTCAAAACAA TCTACTCTGA ATTTCGACTC TCCACTCTAT 
GTAGGAGGCA TGCCTGGGAA AAATAACGTG GCATCCCTGC GCCAGGCCCC TGGGCAAAAT 
GGCACCAGCT TCCATGGCTG TATCCGGAAC CTTTACATTA ACAGTGAGCT GCAGGACTTC 
CGGAAAATGC CTATGCAAAC CGGAATTCTG CCTGGCTGTG AACCATGCCA CAAGAAAGTA 
TGTGCCCATG GCATGTGCCA GCCCAGCAGC CAATCAGGCT TCACCTGTGA ATGTGAGGAA 
GGGTGGATGG GGCCCCTCTG TGACCAGAGA ACCAATGATC CCTGCCTCGG AAACAAATGT 
GTGCATGGGA CCTGCCTGCC CATCAATGCC TTCTCCTATA GTTGCAAGTG CCTGGAGGGC 
CATGGCGGTG TCCTCTGTGA TGAAGAAGAA GATCTCTTTA ACCCCTGCCA GATGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCTGGA GTGGGCCAGC CCTATTGTGA ATGCAACAGT 
GGATTCACCG GGGACAGCTG TGATAGAGAA ATTTCTTGTC GAGGGGAACG GATAAGGGAC 
TATTACCAGA AGCAGCAGGG TTACGCTGCC TGTCAAACAA CTAAGAAAGT ATCTCGCTTG 
GAATGCAGAG GCGGGTGCGC TGGAGGCCAG TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG 
AAATACTCTT TCGAATGCAC AGATGGCTCC TCATTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GCGCGAGATG TGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( NM_178804.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_848919.3 Mus musculus slit guidance ligand 2 (Slit2), transcript variant 3, mRNA.

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CloneID	OMu17705
Clone ID Related Accession (Same CDS sequence)	NM_178804.5
Accession Version	NM_178804.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK053182.1, BC079911.1, AK033292.1, AC101004.6, AW556809.1 and BE944330.1. On Sep 11, 2015 this sequence version replaced NM_178804.4. Transcript Variant: This variant (3) lacks three alternate in-frame exons compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF144628.1, BC079911.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CAGACTTGGC TTGTCCTGAG 
AAGTGTCGCT GTGAAGGGAC CACAGTAGAC TGCTCCAATC AAAGACTCAA CAAAATCCCT 
GACCATATTC CCCAGTACAC AGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGTTA 
GAAGCCACGG GAATATTTAA GAAACTTCCT CAGTTACGTA AAATCAACTT TAGCAACAAT 
AAGATCACGG ATATCGAGGA GGGTGCATTT GAAGGCGCGT CTGGTGTGAA TGAAATTCTT 
CTCACCAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAAGGACT GGAGAGCCTC 
AAAACATTGA TGCTGAGAAG TAATCGAATA AGCTGTGTTG GGAACGACAG TTTCATAGGA 
CTCGGCTCTG TGCGTCTGCT CTCTTTATAT GACAATCAAA TTACCACAGT GGCACCAGGA 
GCATTTGATT CTCTCCATTC ATTATCCACT CTAAACCTCT TGGCCAATCC TTTCAACTGT 
AACTGTCACC TGGCATGGCT GGGAGAATGG CTCAGAAGGA AAAGAATTGT AACAGGAAAT 
CCTCGATGCC AAAAACCCTA CTTCCTGAAG GAAATCCCAA TCCAGGATGT AGCCATTCAG 
GACTTCACCT GTGATGATGG AAATGATGAC AATAGTTGCT CTCCACTCTC CCGTTGTCCT 
TCTGAATGTA CCTGCTTGGA TACAGTGGTA CGATGTAGCA ACAAGGGCTT GAAGGTTTTG 
CCTAAAGGTA TTCCAAAAGA TGTCACAGAG CTGTATCTGG ATGGGAACCA GTTTACGCTG 
GTCCCGAAGG AACTCTCTAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACCGA 
ATAAGCACCC TTTCCAATCA AAGCTTCAGC AACATGACCC AGCTTCTCAC CTTAATCCTC 
AGTTACAACC GTCTGAGATG TATCCCTCCA CGAACCTTTG ATGGATTGAA GTCTCTTCGG 
TTACTGTCTT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCCTT CAATGACTTG 
TCAGCCTTGT CACACTTAGC GATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAACCTG GAATTGCACG CTGTGCCGGC 
CCTGGAGAAA TGGCAGATAA ATTATTACTC ACTACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCCGTGG ATATCACTAT TCAAGCCAAG TGTAATCCCT GCTTATCAAA TCCATGTAAA 
AATGATGGCA CCTGTAACAA TGACCCCGTT GATTTTTATC GATGTACCTG CCCATATGGA 
TTCAAGGGTC AGGACTGTGA TGTCCCCATT CATGCTTGTA TCAGTAATCC ATGTAAACAT 
GGAGGAACTT GTCACTTAAA GGAAGGAGAG AATGCTGGAT TCTGGTGCAC TTGTGCTGAT 
GGGTTTGAAG GAGAAAACTG TGAAGTCAAT ATTGATGATT GTGAAGATAA TGATTGTGAA 
AATAATTCTA CATGCGTTGA TGGAATTAAC AACTACACAT GTCTTTGCCC ACCGGAATAC 
ACAGGTGAAC TGTGTGAGGA AAAGCTGGAC TTCTGTGCAC AAGACTTGAA TCCCTGCCAG 
CATGACTCCA AGTGCATCCT GACTCCAAAG GGATTCAAGT GTGACTGCAC TCCAGGATAC 
ATTGGTGAGC ACTGTGACAT TGACTTTGAT GACTGCCAAG ATAACAAGTG TAAAAACGGT 
GCTCACTGCA CAGATGCCGT GAACGGATAC ACGTGCGTCT GTCCTGAAGG CTACAGTGGC 
TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGCA CCAGCCCCTG TGATAATTTT 
GATTGCCAGA ATGGAGCCCA GTGTATCATC AGGATAAATG AACCAATATG CCAGTGTTTG 
CCTGGCTACC TGGGAGAGAA GTGTGAGAAA TTGGTCAGTG TGAATTTTGT AAACAAAGAG 
TCCTATCTTC AGATTCCTTC AGCCAAGGTT CGGCCTCAGA CAAACATCAC ACTTCAGATT 
GCCACAGATG AAGACAGCGG CATCCTCTTG TATAAAGGTG ACAAAGACCA CATTGCCGTG 
GAACTCTATA GAGGGCGAGT TCGAGCCAGC TATGACACCG GCTCTCATCC GGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTGGAGCT ACTGACCCTG 
GATTCCAGTC TTTCCCTCTC TGTGGATGGA GGAAGCCCTA AAGTCATCAC CAATTTGTCA 
AAACAATCTA CTCTGAATTT CGACTCTCCA CTCTATGTAG GAGGCATGCC TGGGAAAAAT 
AACGTGGCAT CCCTGCGCCA GGCCCCTGGG CAAAATGGCA CCAGCTTCCA TGGCTGTATC 
CGGAACCTTT ACATTAACAG TGAGCTGCAG GACTTCCGGA AAATGCCTAT GCAAACCGGA 
ATTCTGCCTG GCTGTGAACC ATGCCACAAG AAAGTATGTG CCCATGGCAT GTGCCAGCCC 
AGCAGCCAAT CAGGCTTCAC CTGTGAATGT GAGGAAGGGT GGATGGGGCC CCTCTGTGAC 
CAGAGAACCA ATGATCCCTG CCTCGGAAAC AAATGTGTGC ATGGGACCTG CCTGCCCATC 
AATGCCTTCT CCTATAGTTG CAAGTGCCTG GAGGGCCATG GCGGTGTCCT CTGTGATGAA 
GAAGAAGATC TCTTTAACCC CTGCCAGATG ATCAAGTGCA AGCATGGGAA GTGCAGGCTT 
TCTGGAGTGG GCCAGCCCTA TTGTGAATGC AACAGTGGAT TCACCGGGGA CAGCTGTGAT 
AGAGAAATTT CTTGTCGAGG GGAACGGATA AGGGACTATT ACCAGAAGCA GCAGGGTTAC 
GCTGCCTGTC AAACAACTAA GAAAGTATCT CGCTTGGAAT GCAGAGGCGG GTGCGCTGGA 
GGCCAGTGCT GTGGACCTCT GAGAAGCAAG AGGCGGAAAT ACTCTTTCGA ATGCACAGAT 
GGCTCCTCAT TTGTGGACGA GGTTGAGAAA GTGGTGAAGT GCGGCTGCGC GAGATGTGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_848919.3
CDS	1886..6451
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	NM_178804.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178804.5

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CAGACTTGGC TTGTCCTGAG 
AAGTGTCGCT GTGAAGGGAC CACAGTAGAC TGCTCCAATC AAAGACTCAA CAAAATCCCT 
GACCATATTC CCCAGTACAC AGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGTTA 
GAAGCCACGG GAATATTTAA GAAACTTCCT CAGTTACGTA AAATCAACTT TAGCAACAAT 
AAGATCACGG ATATCGAGGA GGGTGCATTT GAAGGCGCGT CTGGTGTGAA TGAAATTCTT 
CTCACCAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAAGGACT GGAGAGCCTC 
AAAACATTGA TGCTGAGAAG TAATCGAATA AGCTGTGTTG GGAACGACAG TTTCATAGGA 
CTCGGCTCTG TGCGTCTGCT CTCTTTATAT GACAATCAAA TTACCACAGT GGCACCAGGA 
GCATTTGATT CTCTCCATTC ATTATCCACT CTAAACCTCT TGGCCAATCC TTTCAACTGT 
AACTGTCACC TGGCATGGCT GGGAGAATGG CTCAGAAGGA AAAGAATTGT AACAGGAAAT 
CCTCGATGCC AAAAACCCTA CTTCCTGAAG GAAATCCCAA TCCAGGATGT AGCCATTCAG 
GACTTCACCT GTGATGATGG AAATGATGAC AATAGTTGCT CTCCACTCTC CCGTTGTCCT 
TCTGAATGTA CCTGCTTGGA TACAGTGGTA CGATGTAGCA ACAAGGGCTT GAAGGTTTTG 
CCTAAAGGTA TTCCAAAAGA TGTCACAGAG CTGTATCTGG ATGGGAACCA GTTTACGCTG 
GTCCCGAAGG AACTCTCTAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACCGA 
ATAAGCACCC TTTCCAATCA AAGCTTCAGC AACATGACCC AGCTTCTCAC CTTAATCCTC 
AGTTACAACC GTCTGAGATG TATCCCTCCA CGAACCTTTG ATGGATTGAA GTCTCTTCGG 
TTACTGTCTT TACATGGAAA TGACATTTCT GTTGTGCCTG AAGGTGCCTT CAATGACTTG 
TCAGCCTTGT CACACTTAGC GATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAACCTG GAATTGCACG CTGTGCCGGC 
CCTGGAGAAA TGGCAGATAA ATTATTACTC ACTACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCCGTGG ATATCACTAT TCAAGCCAAG TGTAATCCCT GCTTATCAAA TCCATGTAAA 
AATGATGGCA CCTGTAACAA TGACCCCGTT GATTTTTATC GATGTACCTG CCCATATGGA 
TTCAAGGGTC AGGACTGTGA TGTCCCCATT CATGCTTGTA TCAGTAATCC ATGTAAACAT 
GGAGGAACTT GTCACTTAAA GGAAGGAGAG AATGCTGGAT TCTGGTGCAC TTGTGCTGAT 
GGGTTTGAAG GAGAAAACTG TGAAGTCAAT ATTGATGATT GTGAAGATAA TGATTGTGAA 
AATAATTCTA CATGCGTTGA TGGAATTAAC AACTACACAT GTCTTTGCCC ACCGGAATAC 
ACAGGTGAAC TGTGTGAGGA AAAGCTGGAC TTCTGTGCAC AAGACTTGAA TCCCTGCCAG 
CATGACTCCA AGTGCATCCT GACTCCAAAG GGATTCAAGT GTGACTGCAC TCCAGGATAC 
ATTGGTGAGC ACTGTGACAT TGACTTTGAT GACTGCCAAG ATAACAAGTG TAAAAACGGT 
GCTCACTGCA CAGATGCCGT GAACGGATAC ACGTGCGTCT GTCCTGAAGG CTACAGTGGC 
TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGCA CCAGCCCCTG TGATAATTTT 
GATTGCCAGA ATGGAGCCCA GTGTATCATC AGGATAAATG AACCAATATG CCAGTGTTTG 
CCTGGCTACC TGGGAGAGAA GTGTGAGAAA TTGGTCAGTG TGAATTTTGT AAACAAAGAG 
TCCTATCTTC AGATTCCTTC AGCCAAGGTT CGGCCTCAGA CAAACATCAC ACTTCAGATT 
GCCACAGATG AAGACAGCGG CATCCTCTTG TATAAAGGTG ACAAAGACCA CATTGCCGTG 
GAACTCTATA GAGGGCGAGT TCGAGCCAGC TATGACACCG GCTCTCATCC GGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTGGAGCT ACTGACCCTG 
GATTCCAGTC TTTCCCTCTC TGTGGATGGA GGAAGCCCTA AAGTCATCAC CAATTTGTCA 
AAACAATCTA CTCTGAATTT CGACTCTCCA CTCTATGTAG GAGGCATGCC TGGGAAAAAT 
AACGTGGCAT CCCTGCGCCA GGCCCCTGGG CAAAATGGCA CCAGCTTCCA TGGCTGTATC 
CGGAACCTTT ACATTAACAG TGAGCTGCAG GACTTCCGGA AAATGCCTAT GCAAACCGGA 
ATTCTGCCTG GCTGTGAACC ATGCCACAAG AAAGTATGTG CCCATGGCAT GTGCCAGCCC 
AGCAGCCAAT CAGGCTTCAC CTGTGAATGT GAGGAAGGGT GGATGGGGCC CCTCTGTGAC 
CAGAGAACCA ATGATCCCTG CCTCGGAAAC AAATGTGTGC ATGGGACCTG CCTGCCCATC 
AATGCCTTCT CCTATAGTTG CAAGTGCCTG GAGGGCCATG GCGGTGTCCT CTGTGATGAA 
GAAGAAGATC TCTTTAACCC CTGCCAGATG ATCAAGTGCA AGCATGGGAA GTGCAGGCTT 
TCTGGAGTGG GCCAGCCCTA TTGTGAATGC AACAGTGGAT TCACCGGGGA CAGCTGTGAT 
AGAGAAATTT CTTGTCGAGG GGAACGGATA AGGGACTATT ACCAGAAGCA GCAGGGTTAC 
GCTGCCTGTC AAACAACTAA GAAAGTATCT CGCTTGGAAT GCAGAGGCGG GTGCGCTGGA 
GGCCAGTGCT GTGGACCTCT GAGAAGCAAG AGGCGGAAAT ACTCTTTCGA ATGCACAGAT 
GGCTCCTCAT TTGTGGACGA GGTTGAGAAA GTGGTGAAGT GCGGCTGCGC GAGATGTGCC 
TCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( NM_001291227.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278156.1 Mus musculus slit guidance ligand 2 (Slit2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17713
Clone ID Related Accession (Same CDS sequence)	NM_001291227.2
Accession Version	NM_001291227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK053182.1, AC113542.13, AC157351.11, AC101004.6, AW556809.1 and BE944330.1. On Sep 11, 2015 this sequence version replaced NM_001291227.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC145487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164132 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAGAAGT GTCGCTGTGA AGGGACCACA 
GTAGACTGCT CCAATCAAAG ACTCAACAAA ATCCCTGACC ATATTCCCCA GTACACAGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA 
CTTCCTCAGT TACGTAAAAT CAACTTTAGC AACAATAAGA TCACGGATAT CGAGGAGGGT 
GCATTTGAAG GCGCGTCTGG TGTGAATGAA ATTCTTCTCA CCAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA AGGACTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT 
CGAATAAGCT GTGTTGGGAA CGACAGTTTC ATAGGACTCG GCTCTGTGCG TCTGCTCTCT 
TTATATGACA ATCAAATTAC CACAGTGGCA CCAGGAGCAT TTGATTCTCT CCATTCATTA 
TCCACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTGGGA 
GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC 
CTGAAGGAAA TCCCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGATGGAAAT 
GATGACAATA GTTGCTCTCC ACTCTCCCGT TGTCCTTCTG AATGTACCTG CTTGGATACA 
GTGGTACGAT GTAGCAACAA GGGCTTGAAG GTTTTGCCTA AAGGTATTCC AAAAGATGTC 
ACAGAGCTGT ATCTGGATGG GAACCAGTTT ACGCTGGTCC CGAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACCGAATAA GCACCCTTTC CAATCAAAGC 
TTCAGCAACA TGACCCAGCT TCTCACCTTA ATCCTCAGTT ACAACCGTCT GAGATGTATC 
CCTCCACGAA CCTTTGATGG ATTGAAGTCT CTTCGGTTAC TGTCTTTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCCTTCAAT GACTTGTCAG CCTTGTCACA CTTAGCGATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AACCTGGAAT TGCACGCTGT GCCGGCCCTG GAGAAATGGC AGATAAATTA 
TTACTCACTA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CCGTGGATAT CACTATTCAA 
GCCAAGTGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCCGTTGATT TTTATCGATG TACCTGCCCA TATGGATTCA AGGGTCAGGA CTGTGATGTC 
CCCATTCATG CTTGTATCAG TAATCCATGT AAACATGGAG GAACTTGTCA CTTAAAGGAA 
GGAGAGAATG CTGGATTCTG GTGCACTTGT GCTGATGGGT TTGAAGGAGA AAACTGTGAA 
GTCAATATTG ATGATTGTGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA 
ATTAACAACT ACACATGTCT TTGCCCACCG GAATACACAG CTGCTAATCT GAATGAGGTG 
GAAAAAGGTG AACTGTGTGA GGAAAAGCTG GACTTCTGTG CACAAGACTT GAATCCCTGC 
CAGCATGACT CCAAGTGCAT CCTGACTCCA AAGGGATTCA AGTGTGACTG CACTCCAGGA 
TACATTGGTG AGCACTGTGA CATTGACTTT GATGACTGCC AAGATAACAA GTGTAAAAAC 
GGTGCTCACT GCACAGATGC CGTGAACGGA TACACGTGCG TCTGTCCTGA AGGCTACAGT 
GGCTTGTTCT GTGAGTTTTC TCCACCCATG GTCCTCCCTC GCACCAGCCC CTGTGATAAT 
TTTGATTGCC AGAATGGAGC CCAGTGTATC ATCAGGATAA ATGAACCAAT ATGCCAGTGT 
TTGCCTGGCT ACCTGGGAGA GAAGTGTGAG AAATTGGTCA GTGTGAATTT TGTAAACAAA 
GAGTCCTATC TTCAGATTCC TTCAGCCAAG GTTCGGCCTC AGACAAACAT CACACTTCAG 
ATTGCCACAG ATGAAGACAG CGGCATCCTC TTGTATAAAG GTGACAAAGA CCACATTGCC 
GTGGAACTCT ATAGAGGGCG AGTTCGAGCC AGCTATGACA CCGGCTCTCA TCCGGCTTCT 
GCCATTTACA GTGTGGAGAC AATCAATGAT GGAAACTTCC ACATTGTGGA GCTACTGACC 
CTGGATTCCA GTCTTTCCCT CTCTGTGGAT GGAGGAAGCC CTAAAGTCAT CACCAATTTG 
TCAAAACAAT CTACTCTGAA TTTCGACTCT CCACTCTATG TAGGAGGCAT GCCTGGGAAA 
AATAACGTGG CATCCCTGCG CCAGGCCCCT GGGCAAAATG GCACCAGCTT CCATGGCTGT 
ATCCGGAACC TTTACATTAA CAGTGAGCTG CAGGACTTCC GGAAAATGCC TATGCAAACC 
GGAATTCTGC CTGGCTGTGA ACCATGCCAC AAGAAAGTAT GTGCCCATGG CATGTGCCAG 
CCCAGCAGCC AATCAGGCTT CACCTGTGAA TGTGAGGAAG GGTGGATGGG GCCCCTCTGT 
GACCAGAGAA CCAATGATCC CTGCCTCGGA AACAAATGTG TGCATGGGAC CTGCCTGCCC 
ATCAATGCCT TCTCCTATAG TTGCAAGTGC CTGGAGGGCC ATGGCGGTGT CCTCTGTGAT 
GAAGAAGAAG ATCTCTTTAA CCCCTGCCAG ATGATCAAGT GCAAGCATGG GAAGTGCAGG 
CTTTCTGGAG TGGGCCAGCC CTATTGTGAA TGCAACAGTG GATTCACCGG GGACAGCTGT 
GATAGAGAAA TTTCTTGTCG AGGGGAACGG ATAAGGGACT ATTACCAGAA GCAGCAGGGT 
TACGCTGCCT GTCAAACAAC TAAGAAAGTA TCTCGCTTGG AATGCAGAGG CGGGTGCGCT 
GGAGGCCAGT GCTGTGGACC TCTGAGAAGC AAGAGGCGGA AATACTCTTT CGAATGCACA 
GATGGCTCCT CATTTGTGGA CGAGGTTGAG AAAGTGGTGA AGTGCGGCTG CGCGAGATGT 
GCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278156.1
CDS	1886..6514
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	NM_001291227.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291227.2

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAGAAGT GTCGCTGTGA AGGGACCACA 
GTAGACTGCT CCAATCAAAG ACTCAACAAA ATCCCTGACC ATATTCCCCA GTACACAGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA 
CTTCCTCAGT TACGTAAAAT CAACTTTAGC AACAATAAGA TCACGGATAT CGAGGAGGGT 
GCATTTGAAG GCGCGTCTGG TGTGAATGAA ATTCTTCTCA CCAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA AGGACTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT 
CGAATAAGCT GTGTTGGGAA CGACAGTTTC ATAGGACTCG GCTCTGTGCG TCTGCTCTCT 
TTATATGACA ATCAAATTAC CACAGTGGCA CCAGGAGCAT TTGATTCTCT CCATTCATTA 
TCCACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTGGGA 
GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC 
CTGAAGGAAA TCCCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGATGGAAAT 
GATGACAATA GTTGCTCTCC ACTCTCCCGT TGTCCTTCTG AATGTACCTG CTTGGATACA 
GTGGTACGAT GTAGCAACAA GGGCTTGAAG GTTTTGCCTA AAGGTATTCC AAAAGATGTC 
ACAGAGCTGT ATCTGGATGG GAACCAGTTT ACGCTGGTCC CGAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACCGAATAA GCACCCTTTC CAATCAAAGC 
TTCAGCAACA TGACCCAGCT TCTCACCTTA ATCCTCAGTT ACAACCGTCT GAGATGTATC 
CCTCCACGAA CCTTTGATGG ATTGAAGTCT CTTCGGTTAC TGTCTTTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCCTTCAAT GACTTGTCAG CCTTGTCACA CTTAGCGATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AACCTGGAAT TGCACGCTGT GCCGGCCCTG GAGAAATGGC AGATAAATTA 
TTACTCACTA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CCGTGGATAT CACTATTCAA 
GCCAAGTGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCCGTTGATT TTTATCGATG TACCTGCCCA TATGGATTCA AGGGTCAGGA CTGTGATGTC 
CCCATTCATG CTTGTATCAG TAATCCATGT AAACATGGAG GAACTTGTCA CTTAAAGGAA 
GGAGAGAATG CTGGATTCTG GTGCACTTGT GCTGATGGGT TTGAAGGAGA AAACTGTGAA 
GTCAATATTG ATGATTGTGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA 
ATTAACAACT ACACATGTCT TTGCCCACCG GAATACACAG CTGCTAATCT GAATGAGGTG 
GAAAAAGGTG AACTGTGTGA GGAAAAGCTG GACTTCTGTG CACAAGACTT GAATCCCTGC 
CAGCATGACT CCAAGTGCAT CCTGACTCCA AAGGGATTCA AGTGTGACTG CACTCCAGGA 
TACATTGGTG AGCACTGTGA CATTGACTTT GATGACTGCC AAGATAACAA GTGTAAAAAC 
GGTGCTCACT GCACAGATGC CGTGAACGGA TACACGTGCG TCTGTCCTGA AGGCTACAGT 
GGCTTGTTCT GTGAGTTTTC TCCACCCATG GTCCTCCCTC GCACCAGCCC CTGTGATAAT 
TTTGATTGCC AGAATGGAGC CCAGTGTATC ATCAGGATAA ATGAACCAAT ATGCCAGTGT 
TTGCCTGGCT ACCTGGGAGA GAAGTGTGAG AAATTGGTCA GTGTGAATTT TGTAAACAAA 
GAGTCCTATC TTCAGATTCC TTCAGCCAAG GTTCGGCCTC AGACAAACAT CACACTTCAG 
ATTGCCACAG ATGAAGACAG CGGCATCCTC TTGTATAAAG GTGACAAAGA CCACATTGCC 
GTGGAACTCT ATAGAGGGCG AGTTCGAGCC AGCTATGACA CCGGCTCTCA TCCGGCTTCT 
GCCATTTACA GTGTGGAGAC AATCAATGAT GGAAACTTCC ACATTGTGGA GCTACTGACC 
CTGGATTCCA GTCTTTCCCT CTCTGTGGAT GGAGGAAGCC CTAAAGTCAT CACCAATTTG 
TCAAAACAAT CTACTCTGAA TTTCGACTCT CCACTCTATG TAGGAGGCAT GCCTGGGAAA 
AATAACGTGG CATCCCTGCG CCAGGCCCCT GGGCAAAATG GCACCAGCTT CCATGGCTGT 
ATCCGGAACC TTTACATTAA CAGTGAGCTG CAGGACTTCC GGAAAATGCC TATGCAAACC 
GGAATTCTGC CTGGCTGTGA ACCATGCCAC AAGAAAGTAT GTGCCCATGG CATGTGCCAG 
CCCAGCAGCC AATCAGGCTT CACCTGTGAA TGTGAGGAAG GGTGGATGGG GCCCCTCTGT 
GACCAGAGAA CCAATGATCC CTGCCTCGGA AACAAATGTG TGCATGGGAC CTGCCTGCCC 
ATCAATGCCT TCTCCTATAG TTGCAAGTGC CTGGAGGGCC ATGGCGGTGT CCTCTGTGAT 
GAAGAAGAAG ATCTCTTTAA CCCCTGCCAG ATGATCAAGT GCAAGCATGG GAAGTGCAGG 
CTTTCTGGAG TGGGCCAGCC CTATTGTGAA TGCAACAGTG GATTCACCGG GGACAGCTGT 
GATAGAGAAA TTTCTTGTCG AGGGGAACGG ATAAGGGACT ATTACCAGAA GCAGCAGGGT 
TACGCTGCCT GTCAAACAAC TAAGAAAGTA TCTCGCTTGG AATGCAGAGG CGGGTGCGCT 
GGAGGCCAGT GCTGTGGACC TCTGAGAAGC AAGAGGCGGA AATACTCTTT CGAATGCACA 
GATGGCTCCT CATTTGTGGA CGAGGTTGAG AAAGTGGTGA AGTGCGGCTG CGCGAGATGT 
GCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( NM_001291228.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278157.1 Mus musculus slit guidance ligand 2 (Slit2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17803
Clone ID Related Accession (Same CDS sequence)	NM_001291228.2
Accession Version	NM_001291228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK053182.1, AC113542.13, AC157351.11, AC101004.6, AW556809.1 and BE944330.1. On Sep 11, 2015 this sequence version replaced NM_001291228.1. Transcript Variant: This variant (2) lacks alternate in-frame exons in the 5' and central coding regions compared to variant 1. It encodes isoform b, which is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660821.84165.1, AK220505.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG 
GCTTGTCCTG AGAAGTGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAGACTC 
AACAAAATCC CTGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAGTTACG TAAAATCAAC 
TTTAGCAACA ATAAGATCAC GGATATCGAG GAGGGTGCAT TTGAAGGCGC GTCTGGTGTG 
AATGAAATTC TTCTCACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA 
CTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT TGGGAACGAC 
AGTTTCATAG GACTCGGCTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA 
GTGGCACCAG GAGCATTTGA TTCTCTCCAT TCATTATCCA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGTCA CCTGGCATGG CTGGGAGAAT GGCTCAGAAG GAAAAGAATT 
GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCCTGA AGGAAATCCC AATCCAGGAT 
GTAGCCATTC AGGACTTCAC CTGTGATGAT GGAAATGATG ACAATAGTTG CTCTCCACTC 
TCCCGTTGTC CTTCTGAATG TACCTGCTTG GATACAGTGG TACGATGTAG CAACAAGGGC 
TTGAAGGTTT TGCCTAAAGG TATTCCAAAA GATGTCACAG AGCTGTATCT GGATGGGAAC 
CAGTTTACGC TGGTCCCGAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACC GAATAAGCAC CCTTTCCAAT CAAAGCTTCA GCAACATGAC CCAGCTTCTC 
ACCTTAATCC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGAACCTT TGATGGATTG 
AAGTCTCTTC GGTTACTGTC TTTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCC 
TTCAATGACT TGTCAGCCTT GTCACACTTA GCGATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCA 
CGCTGTGCCG GCCCTGGAGA AATGGCAGAT AAATTATTAC TCACTACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCCGT GGATATCACT ATTCAAGCCA AGTGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCCG TTGATTTTTA TCGATGTACC 
TGCCCATATG GATTCAAGGG TCAGGACTGT GATGTCCCCA TTCATGCTTG TATCAGTAAT 
CCATGTAAAC ATGGAGGAAC TTGTCACTTA AAGGAAGGAG AGAATGCTGG ATTCTGGTGC 
ACTTGTGCTG ATGGGTTTGA AGGAGAAAAC TGTGAAGTCA ATATTGATGA TTGTGAAGAT 
AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC ATGTCTTTGC 
CCACCGGAAT ACACAGGTGA ACTGTGTGAG GAAAAGCTGG ACTTCTGTGC ACAAGACTTG 
AATCCCTGCC AGCATGACTC CAAGTGCATC CTGACTCCAA AGGGATTCAA GTGTGACTGC 
ACTCCAGGAT ACATTGGTGA GCACTGTGAC ATTGACTTTG ATGACTGCCA AGATAACAAG 
TGTAAAAACG GTGCTCACTG CACAGATGCC GTGAACGGAT ACACGTGCGT CTGTCCTGAA 
GGCTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG CACCAGCCCC 
TGTGATAATT TTGATTGCCA GAATGGAGCC CAGTGTATCA TCAGGATAAA TGAACCAATA 
TGCCAGTGTT TGCCTGGCTA CCTGGGAGAG AAGTGTGAGA AATTGGTCAG TGTGAATTTT 
GTAAACAAAG AGTCCTATCT TCAGATTCCT TCAGCCAAGG TTCGGCCTCA GACAAACATC 
ACACTTCAGA TTGCCACAGA TGAAGACAGC GGCATCCTCT TGTATAAAGG TGACAAAGAC 
CACATTGCCG TGGAACTCTA TAGAGGGCGA GTTCGAGCCA GCTATGACAC CGGCTCTCAT 
CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTGGAG 
CTACTGACCC TGGATTCCAG TCTTTCCCTC TCTGTGGATG GAGGAAGCCC TAAAGTCATC 
ACCAATTTGT CAAAACAATC TACTCTGAAT TTCGACTCTC CACTCTATGT AGGAGGCATG 
CCTGGGAAAA ATAACGTGGC ATCCCTGCGC CAGGCCCCTG GGCAAAATGG CACCAGCTTC 
CATGGCTGTA TCCGGAACCT TTACATTAAC AGTGAGCTGC AGGACTTCCG GAAAATGCCT 
ATGCAAACCG GAATTCTGCC TGGCTGTGAA CCATGCCACA AGAAAGTATG TGCCCATGGC 
ATGTGCCAGC CCAGCAGCCA ATCAGGCTTC ACCTGTGAAT GTGAGGAAGG GTGGATGGGG 
CCCCTCTGTG ACCAGAGAAC CAATGATCCC TGCCTCGGAA ACAAATGTGT GCATGGGACC 
TGCCTGCCCA TCAATGCCTT CTCCTATAGT TGCAAGTGCC TGGAGGGCCA TGGCGGTGTC 
CTCTGTGATG AAGAAGAAGA TCTCTTTAAC CCCTGCCAGA TGATCAAGTG CAAGCATGGG 
AAGTGCAGGC TTTCTGGAGT GGGCCAGCCC TATTGTGAAT GCAACAGTGG ATTCACCGGG 
GACAGCTGTG ATAGAGAAAT TTCTTGTCGA GGGGAACGGA TAAGGGACTA TTACCAGAAG 
CAGCAGGGTT ACGCTGCCTG TCAAACAACT AAGAAAGTAT CTCGCTTGGA ATGCAGAGGC 
GGGTGCGCTG GAGGCCAGTG CTGTGGACCT CTGAGAAGCA AGAGGCGGAA ATACTCTTTC 
GAATGCACAG ATGGCTCCTC ATTTGTGGAC GAGGTTGAGA AAGTGGTGAA GTGCGGCTGC 
GCGAGATGTG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278157.1
CDS	1886..6463
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	NM_001291228.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit guidance ligand 2 (Slit2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291228.2

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG 
GCTTGTCCTG AGAAGTGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAGACTC 
AACAAAATCC CTGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAGTTACG TAAAATCAAC 
TTTAGCAACA ATAAGATCAC GGATATCGAG GAGGGTGCAT TTGAAGGCGC GTCTGGTGTG 
AATGAAATTC TTCTCACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA 
CTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT TGGGAACGAC 
AGTTTCATAG GACTCGGCTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA 
GTGGCACCAG GAGCATTTGA TTCTCTCCAT TCATTATCCA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGTCA CCTGGCATGG CTGGGAGAAT GGCTCAGAAG GAAAAGAATT 
GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCCTGA AGGAAATCCC AATCCAGGAT 
GTAGCCATTC AGGACTTCAC CTGTGATGAT GGAAATGATG ACAATAGTTG CTCTCCACTC 
TCCCGTTGTC CTTCTGAATG TACCTGCTTG GATACAGTGG TACGATGTAG CAACAAGGGC 
TTGAAGGTTT TGCCTAAAGG TATTCCAAAA GATGTCACAG AGCTGTATCT GGATGGGAAC 
CAGTTTACGC TGGTCCCGAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACC GAATAAGCAC CCTTTCCAAT CAAAGCTTCA GCAACATGAC CCAGCTTCTC 
ACCTTAATCC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGAACCTT TGATGGATTG 
AAGTCTCTTC GGTTACTGTC TTTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCC 
TTCAATGACT TGTCAGCCTT GTCACACTTA GCGATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCA 
CGCTGTGCCG GCCCTGGAGA AATGGCAGAT AAATTATTAC TCACTACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCCGT GGATATCACT ATTCAAGCCA AGTGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCCG TTGATTTTTA TCGATGTACC 
TGCCCATATG GATTCAAGGG TCAGGACTGT GATGTCCCCA TTCATGCTTG TATCAGTAAT 
CCATGTAAAC ATGGAGGAAC TTGTCACTTA AAGGAAGGAG AGAATGCTGG ATTCTGGTGC 
ACTTGTGCTG ATGGGTTTGA AGGAGAAAAC TGTGAAGTCA ATATTGATGA TTGTGAAGAT 
AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC ATGTCTTTGC 
CCACCGGAAT ACACAGGTGA ACTGTGTGAG GAAAAGCTGG ACTTCTGTGC ACAAGACTTG 
AATCCCTGCC AGCATGACTC CAAGTGCATC CTGACTCCAA AGGGATTCAA GTGTGACTGC 
ACTCCAGGAT ACATTGGTGA GCACTGTGAC ATTGACTTTG ATGACTGCCA AGATAACAAG 
TGTAAAAACG GTGCTCACTG CACAGATGCC GTGAACGGAT ACACGTGCGT CTGTCCTGAA 
GGCTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG CACCAGCCCC 
TGTGATAATT TTGATTGCCA GAATGGAGCC CAGTGTATCA TCAGGATAAA TGAACCAATA 
TGCCAGTGTT TGCCTGGCTA CCTGGGAGAG AAGTGTGAGA AATTGGTCAG TGTGAATTTT 
GTAAACAAAG AGTCCTATCT TCAGATTCCT TCAGCCAAGG TTCGGCCTCA GACAAACATC 
ACACTTCAGA TTGCCACAGA TGAAGACAGC GGCATCCTCT TGTATAAAGG TGACAAAGAC 
CACATTGCCG TGGAACTCTA TAGAGGGCGA GTTCGAGCCA GCTATGACAC CGGCTCTCAT 
CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTGGAG 
CTACTGACCC TGGATTCCAG TCTTTCCCTC TCTGTGGATG GAGGAAGCCC TAAAGTCATC 
ACCAATTTGT CAAAACAATC TACTCTGAAT TTCGACTCTC CACTCTATGT AGGAGGCATG 
CCTGGGAAAA ATAACGTGGC ATCCCTGCGC CAGGCCCCTG GGCAAAATGG CACCAGCTTC 
CATGGCTGTA TCCGGAACCT TTACATTAAC AGTGAGCTGC AGGACTTCCG GAAAATGCCT 
ATGCAAACCG GAATTCTGCC TGGCTGTGAA CCATGCCACA AGAAAGTATG TGCCCATGGC 
ATGTGCCAGC CCAGCAGCCA ATCAGGCTTC ACCTGTGAAT GTGAGGAAGG GTGGATGGGG 
CCCCTCTGTG ACCAGAGAAC CAATGATCCC TGCCTCGGAA ACAAATGTGT GCATGGGACC 
TGCCTGCCCA TCAATGCCTT CTCCTATAGT TGCAAGTGCC TGGAGGGCCA TGGCGGTGTC 
CTCTGTGATG AAGAAGAAGA TCTCTTTAAC CCCTGCCAGA TGATCAAGTG CAAGCATGGG 
AAGTGCAGGC TTTCTGGAGT GGGCCAGCCC TATTGTGAAT GCAACAGTGG ATTCACCGGG 
GACAGCTGTG ATAGAGAAAT TTCTTGTCGA GGGGAACGGA TAAGGGACTA TTACCAGAAG 
CAGCAGGGTT ACGCTGCCTG TCAAACAACT AAGAAAGTAT CTCGCTTGGA ATGCAGAGGC 
GGGTGCGCTG GAGGCCAGTG CTGTGGACCT CTGAGAAGCA AGAGGCGGAA ATACTCTTTC 
GAATGCACAG ATGGCTCCTC ATTTGTGGAC GAGGTTGAGA AAGTGGTGAA GTGCGGCTGC 
GCGAGATGTG CCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503813.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503876.1 Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X1, mRNA.

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CloneID	OMu34990
Clone ID Related Accession (Same CDS sequence)	XM_006503813.4 , XM_006503813.3
Accession Version	XM_006503813.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503813.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGCT GCTAATCTGA ATGAGGTGGA AAAAGGTGAA 
CTGTGTGAGG AAAAGCTGGA CTTCTGTGCA CAAGACTTGA ATCCCTGCCA GCATGACTCC 
AAGTGCATCC TGACTCCAAA GGGATTCAAG TGTGACTGCA CTCCAGGATA CATTGGTGAG 
CACTGTGACA TTGACTTTGA TGACTGCCAA GATAACAAGT GTAAAAACGG TGCTCACTGC 
ACAGATGCCG TGAACGGATA CACGTGCGTC TGTCCTGAAG GCTACAGTGG CTTGTTCTGT 
GAGTTTTCTC CACCCATGGT CCTCCCTCGC ACCAGCCCCT GTGATAATTT TGATTGCCAG 
AATGGAGCCC AGTGTATCAT CAGGATAAAT GAACCAATAT GCCAGTGTTT GCCTGGCTAC 
CTGGGAGAGA AGTGTGAGAA ATTGGTCAGT GTGAATTTTG TAAACAAAGA GTCCTATCTT 
CAGATTCCTT CAGCCAAGGT TCGGCCTCAG ACAAACATCA CACTTCAGAT TGCCACAGAT 
GAAGACAGCG GCATCCTCTT GTATAAAGGT GACAAAGACC ACATTGCCGT GGAACTCTAT 
AGAGGGCGAG TTCGAGCCAG CTATGACACC GGCTCTCATC CGGCTTCTGC CATTTACAGT 
GTGGAGACAA TCAATGATGG AAACTTCCAC ATTGTGGAGC TACTGACCCT GGATTCCAGT 
CTTTCCCTCT CTGTGGATGG AGGAAGCCCT AAAGTCATCA CCAATTTGTC AAAACAATCT 
ACTCTGAATT TCGACTCTCC ACTCTATGTA GGAGGCATGC CTGGGAAAAA TAACGTGGCA 
TCCCTGCGCC AGGCCCCTGG GCAAAATGGC ACCAGCTTCC ATGGCTGTAT CCGGAACCTT 
TACATTAACA GTGAGCTGCA GGACTTCCGG AAAATGCCTA TGCAAACCGG AATTCTGCCT 
GGCTGTGAAC CATGCCACAA GAAAGTATGT GCCCATGGCA TGTGCCAGCC CAGCAGCCAA 
TCAGGCTTCA CCTGTGAATG TGAGGAAGGG TGGATGGGGC CCCTCTGTGA CCAGAGAACC 
AATGATCCCT GCCTCGGAAA CAAATGTGTG CATGGGACCT GCCTGCCCAT CAATGCCTTC 
TCCTATAGTT GCAAGTGCCT GGAGGGCCAT GGCGGTGTCC TCTGTGATGA AGAAGAAGAT 
CTCTTTAACC CCTGCCAGAT GATCAAGTGC AAGCATGGGA AGTGCAGGCT TTCTGGAGTG 
GGCCAGCCCT ATTGTGAATG CAACAGTGGA TTCACCGGGG ACAGCTGTGA TAGAGAAATT 
TCTTGTCGAG GGGAACGGAT AAGGGACTAT TACCAGAAGC AGCAGGGTTA CGCTGCCTGT 
CAAACAACTA AGAAAGTATC TCGCTTGGAA TGCAGAGGCG GGTGCGCTGG AGGCCAGTGC 
TGTGGACCTC TGAGAAGCAA GAGGCGGAAA TACTCTTTCG AATGCACAGA TGGCTCCTCA 
TTTGTGGACG AGGTTGAGAA AGTGGTGAAG TGCGGCTGCG CGAGATGTGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503876.1
CDS	1938..6554
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503813.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503813.3

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGCT GCTAATCTGA ATGAGGTGGA AAAAGGTGAA 
CTGTGTGAGG AAAAGCTGGA CTTCTGTGCA CAAGACTTGA ATCCCTGCCA GCATGACTCC 
AAGTGCATCC TGACTCCAAA GGGATTCAAG TGTGACTGCA CTCCAGGATA CATTGGTGAG 
CACTGTGACA TTGACTTTGA TGACTGCCAA GATAACAAGT GTAAAAACGG TGCTCACTGC 
ACAGATGCCG TGAACGGATA CACGTGCGTC TGTCCTGAAG GCTACAGTGG CTTGTTCTGT 
GAGTTTTCTC CACCCATGGT CCTCCCTCGC ACCAGCCCCT GTGATAATTT TGATTGCCAG 
AATGGAGCCC AGTGTATCAT CAGGATAAAT GAACCAATAT GCCAGTGTTT GCCTGGCTAC 
CTGGGAGAGA AGTGTGAGAA ATTGGTCAGT GTGAATTTTG TAAACAAAGA GTCCTATCTT 
CAGATTCCTT CAGCCAAGGT TCGGCCTCAG ACAAACATCA CACTTCAGAT TGCCACAGAT 
GAAGACAGCG GCATCCTCTT GTATAAAGGT GACAAAGACC ACATTGCCGT GGAACTCTAT 
AGAGGGCGAG TTCGAGCCAG CTATGACACC GGCTCTCATC CGGCTTCTGC CATTTACAGT 
GTGGAGACAA TCAATGATGG AAACTTCCAC ATTGTGGAGC TACTGACCCT GGATTCCAGT 
CTTTCCCTCT CTGTGGATGG AGGAAGCCCT AAAGTCATCA CCAATTTGTC AAAACAATCT 
ACTCTGAATT TCGACTCTCC ACTCTATGTA GGAGGCATGC CTGGGAAAAA TAACGTGGCA 
TCCCTGCGCC AGGCCCCTGG GCAAAATGGC ACCAGCTTCC ATGGCTGTAT CCGGAACCTT 
TACATTAACA GTGAGCTGCA GGACTTCCGG AAAATGCCTA TGCAAACCGG AATTCTGCCT 
GGCTGTGAAC CATGCCACAA GAAAGTATGT GCCCATGGCA TGTGCCAGCC CAGCAGCCAA 
TCAGGCTTCA CCTGTGAATG TGAGGAAGGG TGGATGGGGC CCCTCTGTGA CCAGAGAACC 
AATGATCCCT GCCTCGGAAA CAAATGTGTG CATGGGACCT GCCTGCCCAT CAATGCCTTC 
TCCTATAGTT GCAAGTGCCT GGAGGGCCAT GGCGGTGTCC TCTGTGATGA AGAAGAAGAT 
CTCTTTAACC CCTGCCAGAT GATCAAGTGC AAGCATGGGA AGTGCAGGCT TTCTGGAGTG 
GGCCAGCCCT ATTGTGAATG CAACAGTGGA TTCACCGGGG ACAGCTGTGA TAGAGAAATT 
TCTTGTCGAG GGGAACGGAT AAGGGACTAT TACCAGAAGC AGCAGGGTTA CGCTGCCTGT 
CAAACAACTA AGAAAGTATC TCGCTTGGAA TGCAGAGGCG GGTGCGCTGG AGGCCAGTGC 
TGTGGACCTC TGAGAAGCAA GAGGCGGAAA TACTCTTTCG AATGCACAGA TGGCTCCTCA 
TTTGTGGACG AGGTTGAGAA AGTGGTGAAG TGCGGCTGCG CGAGATGTGC CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503814.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503877.1 Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X2, mRNA.

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CloneID	OMu34991
Clone ID Related Accession (Same CDS sequence)	XM_006503814.3 , XM_006503814.4
Accession Version	XM_006503814.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503814.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG 
GCTTGTCCTG AGAAGTGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAGACTC 
AACAAAATCC CTGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAGTTACG TAAAATCAAC 
TTTAGCAACA ATAAGATCAC GGATATCGAG GAGGGTGCAT TTGAAGGCGC GTCTGGTGTG 
AATGAAATTC TTCTCACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA 
CTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT TGGGAACGAC 
AGTTTCATAG GACTCGGCTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA 
GTGGCACCAG GAGCATTTGA TTCTCTCCAT TCATTATCCA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGTCA CCTGGCATGG CTGGGAGAAT GGCTCAGAAG GAAAAGAATT 
GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCCTGA AGGAAATCCC AATCCAGGAT 
GTAGCCATTC AGGACTTCAC CTGTGATGAT GGAAATGATG ACAATAGTTG CTCTCCACTC 
TCCCGTTGTC CTTCTGAATG TACCTGCTTG GATACAGTGG TACGATGTAG CAACAAGGGC 
TTGAAGGTTT TGCCTAAAGG TATTCCAAAA GATGTCACAG AGCTGTATCT GGATGGGAAC 
CAGTTTACGC TGGTCCCGAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACC GAATAAGCAC CCTTTCCAAT CAAAGCTTCA GCAACATGAC CCAGCTTCTC 
ACCTTAATCC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGAACCTT TGATGGATTG 
AAGTCTCTTC GGTTACTGTC TTTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCC 
TTCAATGACT TGTCAGCCTT GTCACACTTA GCGATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCA 
CGCTGTGCCG GCCCTGGAGA AATGGCAGAT AAATTATTAC TCACTACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCCGT GGATATCACT ATTCAAGCCA AGTGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCCG TTGATTTTTA TCGATGTACC 
TGCCCATATG GATTCAAGGG TCAGGACTGT GATGTCCCCA TTCATGCTTG TATCAGTAAT 
CCATGTAAAC ATGGAGGAAC TTGTCACTTA AAGGAAGGAG AGAATGCTGG ATTCTGGTGC 
ACTTGTGCTG ATGGGTTTGA AGGAGAAAAC TGTGAAGTCA ATATTGATGA TTGTGAAGAT 
AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC ATGTCTTTGC 
CCACCGGAAT ACACAGCTGC TAATCTGAAT GAGGTGGAAA AAGGTGAACT GTGTGAGGAA 
AAGCTGGACT TCTGTGCACA AGACTTGAAT CCCTGCCAGC ATGACTCCAA GTGCATCCTG 
ACTCCAAAGG GATTCAAGTG TGACTGCACT CCAGGATACA TTGGTGAGCA CTGTGACATT 
GACTTTGATG ACTGCCAAGA TAACAAGTGT AAAAACGGTG CTCACTGCAC AGATGCCGTG 
AACGGATACA CGTGCGTCTG TCCTGAAGGC TACAGTGGCT TGTTCTGTGA GTTTTCTCCA 
CCCATGGTCC TCCCTCGCAC CAGCCCCTGT GATAATTTTG ATTGCCAGAA TGGAGCCCAG 
TGTATCATCA GGATAAATGA ACCAATATGC CAGTGTTTGC CTGGCTACCT GGGAGAGAAG 
TGTGAGAAAT TGGTCAGTGT GAATTTTGTA AACAAAGAGT CCTATCTTCA GATTCCTTCA 
GCCAAGGTTC GGCCTCAGAC AAACATCACA CTTCAGATTG CCACAGATGA AGACAGCGGC 
ATCCTCTTGT ATAAAGGTGA CAAAGACCAC ATTGCCGTGG AACTCTATAG AGGGCGAGTT 
CGAGCCAGCT ATGACACCGG CTCTCATCCG GCTTCTGCCA TTTACAGTGT GGAGACAATC 
AATGATGGAA ACTTCCACAT TGTGGAGCTA CTGACCCTGG ATTCCAGTCT TTCCCTCTCT 
GTGGATGGAG GAAGCCCTAA AGTCATCACC AATTTGTCAA AACAATCTAC TCTGAATTTC 
GACTCTCCAC TCTATGTAGG AGGCATGCCT GGGAAAAATA ACGTGGCATC CCTGCGCCAG 
GCCCCTGGGC AAAATGGCAC CAGCTTCCAT GGCTGTATCC GGAACCTTTA CATTAACAGT 
GAGCTGCAGG ACTTCCGGAA AATGCCTATG CAAACCGGAA TTCTGCCTGG CTGTGAACCA 
TGCCACAAGA AAGTATGTGC CCATGGCATG TGCCAGCCCA GCAGCCAATC AGGCTTCACC 
TGTGAATGTG AGGAAGGGTG GATGGGGCCC CTCTGTGACC AGAGAACCAA TGATCCCTGC 
CTCGGAAACA AATGTGTGCA TGGGACCTGC CTGCCCATCA ATGCCTTCTC CTATAGTTGC 
AAGTGCCTGG AGGGCCATGG CGGTGTCCTC TGTGATGAAG AAGAAGATCT CTTTAACCCC 
TGCCAGATGA TCAAGTGCAA GCATGGGAAG TGCAGGCTTT CTGGAGTGGG CCAGCCCTAT 
TGTGAATGCA ACAGTGGATT CACCGGGGAC AGCTGTGATA GAGAAATTTC TTGTCGAGGG 
GAACGGATAA GGGACTATTA CCAGAAGCAG CAGGGTTACG CTGCCTGTCA AACAACTAAG 
AAAGTATCTC GCTTGGAATG CAGAGGCGGG TGCGCTGGAG GCCAGTGCTG TGGACCTCTG 
AGAAGCAAGA GGCGGAAATA CTCTTTCGAA TGCACAGATG GCTCCTCATT TGTGGACGAG 
GTTGAGAAAG TGGTGAAGTG CGGCTGCGCG AGATGTGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503877.1
CDS	1939..6543
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503814.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503814.3

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG 
GCTTGTCCTG AGAAGTGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAGACTC 
AACAAAATCC CTGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA 
TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAGTTACG TAAAATCAAC 
TTTAGCAACA ATAAGATCAC GGATATCGAG GAGGGTGCAT TTGAAGGCGC GTCTGGTGTG 
AATGAAATTC TTCTCACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA 
CTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT TGGGAACGAC 
AGTTTCATAG GACTCGGCTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA 
GTGGCACCAG GAGCATTTGA TTCTCTCCAT TCATTATCCA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGTCA CCTGGCATGG CTGGGAGAAT GGCTCAGAAG GAAAAGAATT 
GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCCTGA AGGAAATCCC AATCCAGGAT 
GTAGCCATTC AGGACTTCAC CTGTGATGAT GGAAATGATG ACAATAGTTG CTCTCCACTC 
TCCCGTTGTC CTTCTGAATG TACCTGCTTG GATACAGTGG TACGATGTAG CAACAAGGGC 
TTGAAGGTTT TGCCTAAAGG TATTCCAAAA GATGTCACAG AGCTGTATCT GGATGGGAAC 
CAGTTTACGC TGGTCCCGAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACC GAATAAGCAC CCTTTCCAAT CAAAGCTTCA GCAACATGAC CCAGCTTCTC 
ACCTTAATCC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGAACCTT TGATGGATTG 
AAGTCTCTTC GGTTACTGTC TTTACATGGA AATGACATTT CTGTTGTGCC TGAAGGTGCC 
TTCAATGACT TGTCAGCCTT GTCACACTTA GCGATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCA 
CGCTGTGCCG GCCCTGGAGA AATGGCAGAT AAATTATTAC TCACTACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCCGT GGATATCACT ATTCAAGCCA AGTGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCCG TTGATTTTTA TCGATGTACC 
TGCCCATATG GATTCAAGGG TCAGGACTGT GATGTCCCCA TTCATGCTTG TATCAGTAAT 
CCATGTAAAC ATGGAGGAAC TTGTCACTTA AAGGAAGGAG AGAATGCTGG ATTCTGGTGC 
ACTTGTGCTG ATGGGTTTGA AGGAGAAAAC TGTGAAGTCA ATATTGATGA TTGTGAAGAT 
AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC ATGTCTTTGC 
CCACCGGAAT ACACAGCTGC TAATCTGAAT GAGGTGGAAA AAGGTGAACT GTGTGAGGAA 
AAGCTGGACT TCTGTGCACA AGACTTGAAT CCCTGCCAGC ATGACTCCAA GTGCATCCTG 
ACTCCAAAGG GATTCAAGTG TGACTGCACT CCAGGATACA TTGGTGAGCA CTGTGACATT 
GACTTTGATG ACTGCCAAGA TAACAAGTGT AAAAACGGTG CTCACTGCAC AGATGCCGTG 
AACGGATACA CGTGCGTCTG TCCTGAAGGC TACAGTGGCT TGTTCTGTGA GTTTTCTCCA 
CCCATGGTCC TCCCTCGCAC CAGCCCCTGT GATAATTTTG ATTGCCAGAA TGGAGCCCAG 
TGTATCATCA GGATAAATGA ACCAATATGC CAGTGTTTGC CTGGCTACCT GGGAGAGAAG 
TGTGAGAAAT TGGTCAGTGT GAATTTTGTA AACAAAGAGT CCTATCTTCA GATTCCTTCA 
GCCAAGGTTC GGCCTCAGAC AAACATCACA CTTCAGATTG CCACAGATGA AGACAGCGGC 
ATCCTCTTGT ATAAAGGTGA CAAAGACCAC ATTGCCGTGG AACTCTATAG AGGGCGAGTT 
CGAGCCAGCT ATGACACCGG CTCTCATCCG GCTTCTGCCA TTTACAGTGT GGAGACAATC 
AATGATGGAA ACTTCCACAT TGTGGAGCTA CTGACCCTGG ATTCCAGTCT TTCCCTCTCT 
GTGGATGGAG GAAGCCCTAA AGTCATCACC AATTTGTCAA AACAATCTAC TCTGAATTTC 
GACTCTCCAC TCTATGTAGG AGGCATGCCT GGGAAAAATA ACGTGGCATC CCTGCGCCAG 
GCCCCTGGGC AAAATGGCAC CAGCTTCCAT GGCTGTATCC GGAACCTTTA CATTAACAGT 
GAGCTGCAGG ACTTCCGGAA AATGCCTATG CAAACCGGAA TTCTGCCTGG CTGTGAACCA 
TGCCACAAGA AAGTATGTGC CCATGGCATG TGCCAGCCCA GCAGCCAATC AGGCTTCACC 
TGTGAATGTG AGGAAGGGTG GATGGGGCCC CTCTGTGACC AGAGAACCAA TGATCCCTGC 
CTCGGAAACA AATGTGTGCA TGGGACCTGC CTGCCCATCA ATGCCTTCTC CTATAGTTGC 
AAGTGCCTGG AGGGCCATGG CGGTGTCCTC TGTGATGAAG AAGAAGATCT CTTTAACCCC 
TGCCAGATGA TCAAGTGCAA GCATGGGAAG TGCAGGCTTT CTGGAGTGGG CCAGCCCTAT 
TGTGAATGCA ACAGTGGATT CACCGGGGAC AGCTGTGATA GAGAAATTTC TTGTCGAGGG 
GAACGGATAA GGGACTATTA CCAGAAGCAG CAGGGTTACG CTGCCTGTCA AACAACTAAG 
AAAGTATCTC GCTTGGAATG CAGAGGCGGG TGCGCTGGAG GCCAGTGCTG TGGACCTCTG 
AGAAGCAAGA GGCGGAAATA CTCTTTCGAA TGCACAGATG GCTCCTCATT TGTGGACGAG 
GTTGAGAAAG TGGTGAAGTG CGGCTGCGCG AGATGTGCCT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503815.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503878.1 Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu34992
Clone ID Related Accession (Same CDS sequence)	XM_006503815.3 , XM_006503815.4
Accession Version	XM_006503815.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAGAAGT GTCGCTGTGA AGGGACCACA 
GTAGACTGCT CCAATCAAAG ACTCAACAAA ATCCCTGACC ATATTCCCCA GTACACAGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA 
CTTCCTCAGT TACGTAAAAT CAACTTTAGC AACAATAAGA TCACGGATAT CGAGGAGGGT 
GCATTTGAAG GCGCGTCTGG TGTGAATGAA ATTCTTCTCA CCAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA AGGACTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT 
CGAATAAGCT GTGTTGGGAA CGACAGTTTC ATAGGACTCG GCTCTGTGCG TCTGCTCTCT 
TTATATGACA ATCAAATTAC CACAGTGGCA CCAGGAGCAT TTGATTCTCT CCATTCATTA 
TCCACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTGGGA 
GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC 
CTGAAGGAAA TCCCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGATGGAAAT 
GATGACAATA GTTGCTCTCC ACTCTCCCGT TGTCCTTCTG AATGTACCTG CTTGGATACA 
GTGGTACGAT GTAGCAACAA GGGCTTGAAG GTTTTGCCTA AAGGTATTCC AAAAGATGTC 
ACAGAGCTGT ATCTGGATGG GAACCAGTTT ACGCTGGTCC CGAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACCGAATAA GCACCCTTTC CAATCAAAGC 
TTCAGCAACA TGACCCAGCT TCTCACCTTA ATCCTCAGTT ACAACCGTCT GAGATGTATC 
CCTCCACGAA CCTTTGATGG ATTGAAGTCT CTTCGGTTAC TGTCTTTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCCTTCAAT GACTTGTCAG CCTTGTCACA CTTAGCGATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AACCTGGAAT TGCACGCTGT GCCGGCCCTG GAGAAATGGC AGATAAATTA 
TTACTCACTA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CCGTGGATAT CACTATTCAA 
GCCAAGTGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCCGTTGATT TTTATCGATG TACCTGCCCA TATGGATTCA AGGGTCAGGA CTGTGATGTC 
CCCATTCATG CTTGTATCAG TAATCCATGT AAACATGGAG GAACTTGTCA CTTAAAGGAA 
GGAGAGAATG CTGGATTCTG GTGCACTTGT GCTGATGGGT TTGAAGGAGA AAACTGTGAA 
GTCAATATTG ATGATTGTGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA 
ATTAACAACT ACACATGTCT TTGCCCACCG GAATACACAG GTGAACTGTG TGAGGAAAAG 
CTGGACTTCT GTGCACAAGA CTTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACT 
CCAAAGGGAT TCAAGTGTGA CTGCACTCCA GGATACATTG GTGAGCACTG TGACATTGAC 
TTTGATGACT GCCAAGATAA CAAGTGTAAA AACGGTGCTC ACTGCACAGA TGCCGTGAAC 
GGATACACGT GCGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC 
ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GCCAGAATGG AGCCCAGTGT 
ATCATCAGGA TAAATGAACC AATATGCCAG TGTTTGCCTG GCTACCTGGG AGAGAAGTGT 
GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC 
AAGGTTCGGC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC 
CTCTTGTATA AAGGTGACAA AGACCACATT GCCGTGGAAC TCTATAGAGG GCGAGTTCGA 
GCCAGCTATG ACACCGGCTC TCATCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GATGGAAACT TCCACATTGT GGAGCTACTG ACCCTGGATT CCAGTCTTTC CCTCTCTGTG 
GATGGAGGAA GCCCTAAAGT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC 
TCTCCACTCT ATGTAGGAGG CATGCCTGGG AAAAATAACG TGGCATCCCT GCGCCAGGCC 
CCTGGGCAAA ATGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAG 
CTGCAGGACT TCCGGAAAAT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC 
CACAAGAAAG TATGTGCCCA TGGCATGTGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT 
GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGCCTC 
GGAAACAAAT GTGTGCATGG GACCTGCCTG CCCATCAATG CCTTCTCCTA TAGTTGCAAG 
TGCCTGGAGG GCCATGGCGG TGTCCTCTGT GATGAAGAAG AAGATCTCTT TAACCCCTGC 
CAGATGATCA AGTGCAAGCA TGGGAAGTGC AGGCTTTCTG GAGTGGGCCA GCCCTATTGT 
GAATGCAACA GTGGATTCAC CGGGGACAGC TGTGATAGAG AAATTTCTTG TCGAGGGGAA 
CGGATAAGGG ACTATTACCA GAAGCAGCAG GGTTACGCTG CCTGTCAAAC AACTAAGAAA 
GTATCTCGCT TGGAATGCAG AGGCGGGTGC GCTGGAGGCC AGTGCTGTGG ACCTCTGAGA 
AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGCT CCTCATTTGT GGACGAGGTT 
GAGAAAGTGG TGAAGTGCGG CTGCGCGAGA TGTGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503878.1
CDS	1938..6539
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503815.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503815.3

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG 
GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCCGCTGCT 
TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA 
AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATCAG GGTCATCCCT 
CCAGGAGCCT TCTCACCATA CAAAAAGCTT AGACGACTAG ACCTGAGCAA CAACCAGATC 
TCTGAACTTG CACCAGATGC CTTCCAAGGA CTGCGCTCTC TGAATTCACT TGTCCTGTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTCGAAG GACTATTTTC CTTGCAGCTA 
CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC 
AACTTGAACC TTCTCTCCTT ATATGACAAT AAGCTTCAGA CGGTTGCCAA GGGCACCTTC 
TCAGCCCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC 
CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT 
TGCACCAGCC CCCGCCGCCT GGCAAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAGAAGT GTCGCTGTGA AGGGACCACA 
GTAGACTGCT CCAATCAAAG ACTCAACAAA ATCCCTGACC ATATTCCCCA GTACACAGCA 
GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA 
CTTCCTCAGT TACGTAAAAT CAACTTTAGC AACAATAAGA TCACGGATAT CGAGGAGGGT 
GCATTTGAAG GCGCGTCTGG TGTGAATGAA ATTCTTCTCA CCAGTAACCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA AGGACTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT 
CGAATAAGCT GTGTTGGGAA CGACAGTTTC ATAGGACTCG GCTCTGTGCG TCTGCTCTCT 
TTATATGACA ATCAAATTAC CACAGTGGCA CCAGGAGCAT TTGATTCTCT CCATTCATTA 
TCCACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTGGGA 
GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC 
CTGAAGGAAA TCCCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGATGGAAAT 
GATGACAATA GTTGCTCTCC ACTCTCCCGT TGTCCTTCTG AATGTACCTG CTTGGATACA 
GTGGTACGAT GTAGCAACAA GGGCTTGAAG GTTTTGCCTA AAGGTATTCC AAAAGATGTC 
ACAGAGCTGT ATCTGGATGG GAACCAGTTT ACGCTGGTCC CGAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACCGAATAA GCACCCTTTC CAATCAAAGC 
TTCAGCAACA TGACCCAGCT TCTCACCTTA ATCCTCAGTT ACAACCGTCT GAGATGTATC 
CCTCCACGAA CCTTTGATGG ATTGAAGTCT CTTCGGTTAC TGTCTTTACA TGGAAATGAC 
ATTTCTGTTG TGCCTGAAGG TGCCTTCAAT GACTTGTCAG CCTTGTCACA CTTAGCGATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG 
GAATATAAGG AACCTGGAAT TGCACGCTGT GCCGGCCCTG GAGAAATGGC AGATAAATTA 
TTACTCACTA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CCGTGGATAT CACTATTCAA 
GCCAAGTGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCCGTTGATT TTTATCGATG TACCTGCCCA TATGGATTCA AGGGTCAGGA CTGTGATGTC 
CCCATTCATG CTTGTATCAG TAATCCATGT AAACATGGAG GAACTTGTCA CTTAAAGGAA 
GGAGAGAATG CTGGATTCTG GTGCACTTGT GCTGATGGGT TTGAAGGAGA AAACTGTGAA 
GTCAATATTG ATGATTGTGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA 
ATTAACAACT ACACATGTCT TTGCCCACCG GAATACACAG GTGAACTGTG TGAGGAAAAG 
CTGGACTTCT GTGCACAAGA CTTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACT 
CCAAAGGGAT TCAAGTGTGA CTGCACTCCA GGATACATTG GTGAGCACTG TGACATTGAC 
TTTGATGACT GCCAAGATAA CAAGTGTAAA AACGGTGCTC ACTGCACAGA TGCCGTGAAC 
GGATACACGT GCGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC 
ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GCCAGAATGG AGCCCAGTGT 
ATCATCAGGA TAAATGAACC AATATGCCAG TGTTTGCCTG GCTACCTGGG AGAGAAGTGT 
GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC 
AAGGTTCGGC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC 
CTCTTGTATA AAGGTGACAA AGACCACATT GCCGTGGAAC TCTATAGAGG GCGAGTTCGA 
GCCAGCTATG ACACCGGCTC TCATCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GATGGAAACT TCCACATTGT GGAGCTACTG ACCCTGGATT CCAGTCTTTC CCTCTCTGTG 
GATGGAGGAA GCCCTAAAGT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC 
TCTCCACTCT ATGTAGGAGG CATGCCTGGG AAAAATAACG TGGCATCCCT GCGCCAGGCC 
CCTGGGCAAA ATGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAG 
CTGCAGGACT TCCGGAAAAT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC 
CACAAGAAAG TATGTGCCCA TGGCATGTGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT 
GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGCCTC 
GGAAACAAAT GTGTGCATGG GACCTGCCTG CCCATCAATG CCTTCTCCTA TAGTTGCAAG 
TGCCTGGAGG GCCATGGCGG TGTCCTCTGT GATGAAGAAG AAGATCTCTT TAACCCCTGC 
CAGATGATCA AGTGCAAGCA TGGGAAGTGC AGGCTTTCTG GAGTGGGCCA GCCCTATTGT 
GAATGCAACA GTGGATTCAC CGGGGACAGC TGTGATAGAG AAATTTCTTG TCGAGGGGAA 
CGGATAAGGG ACTATTACCA GAAGCAGCAG GGTTACGCTG CCTGTCAAAC AACTAAGAAA 
GTATCTCGCT TGGAATGCAG AGGCGGGTGC GCTGGAGGCC AGTGCTGTGG ACCTCTGAGA 
AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGCT CCTCATTTGT GGACGAGGTT 
GAGAAAGTGG TGAAGTGCGG CTGCGCGAGA TGTGCCTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slit2 ( XM_006503816.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006503879.1 Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu34993
Clone ID Related Accession (Same CDS sequence)	XM_006503816.4 , XM_006503816.3
Accession Version	XM_006503816.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	slit homolog 2 protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006503816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGGT GAACTGTGTG AGGAAAAGCT GGACTTCTGT 
GCACAAGACT TGAATCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACTCC AAAGGGATTC 
AAGTGTGACT GCACTCCAGG ATACATTGGT GAGCACTGTG ACATTGACTT TGATGACTGC 
CAAGATAACA AGTGTAAAAA CGGTGCTCAC TGCACAGATG CCGTGAACGG ATACACGTGC 
GTCTGTCCTG AAGGCTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGCACCAGCC CCTGTGATAA TTTTGATTGC CAGAATGGAG CCCAGTGTAT CATCAGGATA 
AATGAACCAA TATGCCAGTG TTTGCCTGGC TACCTGGGAG AGAAGTGTGA GAAATTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCCTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACAAACA TCACACTTCA GATTGCCACA GATGAAGACA GCGGCATCCT CTTGTATAAA 
GGTGACAAAG ACCACATTGC CGTGGAACTC TATAGAGGGC GAGTTCGAGC CAGCTATGAC 
ACCGGCTCTC ATCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AGCTACTGAC CCTGGATTCC AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC 
CCTAAAGTCA TCACCAATTT GTCAAAACAA TCTACTCTGA ATTTCGACTC TCCACTCTAT 
GTAGGAGGCA TGCCTGGGAA AAATAACGTG GCATCCCTGC GCCAGGCCCC TGGGCAAAAT 
GGCACCAGCT TCCATGGCTG TATCCGGAAC CTTTACATTA ACAGTGAGCT GCAGGACTTC 
CGGAAAATGC CTATGCAAAC CGGAATTCTG CCTGGCTGTG AACCATGCCA CAAGAAAGTA 
TGTGCCCATG GCATGTGCCA GCCCAGCAGC CAATCAGGCT TCACCTGTGA ATGTGAGGAA 
GGGTGGATGG GGCCCCTCTG TGACCAGAGA ACCAATGATC CCTGCCTCGG AAACAAATGT 
GTGCATGGGA CCTGCCTGCC CATCAATGCC TTCTCCTATA GTTGCAAGTG CCTGGAGGGC 
CATGGCGGTG TCCTCTGTGA TGAAGAAGAA GATCTCTTTA ACCCCTGCCA GATGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCTGGA GTGGGCCAGC CCTATTGTGA ATGCAACAGT 
GGATTCACCG GGGACAGCTG TGATAGAGAA ATTTCTTGTC GAGGGGAACG GATAAGGGAC 
TATTACCAGA AGCAGCAGGG TTACGCTGCC TGTCAAACAA CTAAGAAAGT ATCTCGCTTG 
GAATGCAGAG GCGGGTGCGC TGGAGGCCAG TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG 
AAATACTCTT TCGAATGCAC AGATGGCTCC TCATTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GCGCGAGATG TGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006503879.1
CDS	1937..6526
Translation	MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS

Target ORF information:

RefSeq Version	XM_006503816.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus slit homolog 2 (Drosophila) (Slit2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006503816.3

ORF Insert Sequence:

ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCGCTGG GGTTAGTGTT GTCGATCTTG 
AACAAGGTGG CGCCGCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACGGTGGAC 
TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGACTG 
GATTTGAATG GAAATAACAT CACGAGGATC ACGAAGATAG ATTTTGCTGG TCTCAGGCAC 
CTCAGAGTTC TTCAGCTCAT GGAGAACAGA ATCAGCACCA TCGAGAGGGG AGCATTCCAG 
GATCTTAAGG AGCTGGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG 
CTGCTGTTTC TCGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAAATTCAA 
GCAATTCCAA GGAAGGCTTT CCGTGGGGCA GTTGACATTA AAAACCTGCA ACTGGATTAC 
AACCAGATCA GCTGCATTGA AGATGGGGCG TTCAGAGCTC TACGAGATCT GGAAGTGCTC 
ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACAT TTCGACTCCA CTCGAACAAC TTGTACTGCG ACTGCCACCT AGCCTGGCTC 
TCAGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC 
CACCTGAGGG GCCACAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC 
CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CCGCTGCTTG TACCTGTAGC 
AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCCATCAGGG TCATCCCTCC AGGAGCCTTC 
TCACCATACA AAAAGCTTAG ACGACTAGAC CTGAGCAACA ACCAGATCTC TGAACTTGCA 
CCAGATGCCT TCCAAGGACT GCGCTCTCTG AATTCACTTG TCCTGTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTCGAAGGA CTATTTTCCT TGCAGCTACT ATTATTGAAT 
GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT 
CTCTCCTTAT ATGACAATAA GCTTCAGACG GTTGCCAAGG GCACCTTCTC AGCCCTCAGA 
GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG 
CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGCCCC 
CGCCGCCTGG CAAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC 
TTTGCAGACT TGGCTTGTCC TGAGAAGTGT CGCTGTGAAG GGACCACAGT AGACTGCTCC 
AATCAAAGAC TCAACAAAAT CCCTGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC 
AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAGTTA 
CGTAAAATCA ACTTTAGCAA CAATAAGATC ACGGATATCG AGGAGGGTGC ATTTGAAGGC 
GCGTCTGGTG TGAATGAAAT TCTTCTCACC AGTAACCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAAG GACTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT 
GTTGGGAACG ACAGTTTCAT AGGACTCGGC TCTGTGCGTC TGCTCTCTTT ATATGACAAT 
CAAATTACCA CAGTGGCACC AGGAGCATTT GATTCTCTCC ATTCATTATC CACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTGGGAGA ATGGCTCAGA 
AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCCT GAAGGAAATC 
CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ATGGAAATGA TGACAATAGT 
TGCTCTCCAC TCTCCCGTTG TCCTTCTGAA TGTACCTGCT TGGATACAGT GGTACGATGT 
AGCAACAAGG GCTTGAAGGT TTTGCCTAAA GGTATTCCAA AAGATGTCAC AGAGCTGTAT 
CTGGATGGGA ACCAGTTTAC GCTGGTCCCG AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CCGAATAAGC ACCCTTTCCA ATCAAAGCTT CAGCAACATG 
ACCCAGCTTC TCACCTTAAT CCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGAACC 
TTTGATGGAT TGAAGTCTCT TCGGTTACTG TCTTTACATG GAAATGACAT TTCTGTTGTG 
CCTGAAGGTG CCTTCAATGA CTTGTCAGCC TTGTCACACT TAGCGATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA 
CCTGGAATTG CACGCTGTGC CGGCCCTGGA GAAATGGCAG ATAAATTATT ACTCACTACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCC GTGGATATCA CTATTCAAGC CAAGTGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC CGTTGATTTT 
TATCGATGTA CCTGCCCATA TGGATTCAAG GGTCAGGACT GTGATGTCCC CATTCATGCT 
TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGTCACT TAAAGGAAGG AGAGAATGCT 
GGATTCTGGT GCACTTGTGC TGATGGGTTT GAAGGAGAAA ACTGTGAAGT CAATATTGAT 
GATTGTGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC 
ACATGTCTTT GCCCACCGGA ATACACAGGT GAACTGTGTG AGGAAAAGCT GGACTTCTGT 
GCACAAGACT TGAATCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACTCC AAAGGGATTC 
AAGTGTGACT GCACTCCAGG ATACATTGGT GAGCACTGTG ACATTGACTT TGATGACTGC 
CAAGATAACA AGTGTAAAAA CGGTGCTCAC TGCACAGATG CCGTGAACGG ATACACGTGC 
GTCTGTCCTG AAGGCTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT 
CGCACCAGCC CCTGTGATAA TTTTGATTGC CAGAATGGAG CCCAGTGTAT CATCAGGATA 
AATGAACCAA TATGCCAGTG TTTGCCTGGC TACCTGGGAG AGAAGTGTGA GAAATTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCCTAT CTTCAGATTC CTTCAGCCAA GGTTCGGCCT 
CAGACAAACA TCACACTTCA GATTGCCACA GATGAAGACA GCGGCATCCT CTTGTATAAA 
GGTGACAAAG ACCACATTGC CGTGGAACTC TATAGAGGGC GAGTTCGAGC CAGCTATGAC 
ACCGGCTCTC ATCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC 
CACATTGTGG AGCTACTGAC CCTGGATTCC AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC 
CCTAAAGTCA TCACCAATTT GTCAAAACAA TCTACTCTGA ATTTCGACTC TCCACTCTAT 
GTAGGAGGCA TGCCTGGGAA AAATAACGTG GCATCCCTGC GCCAGGCCCC TGGGCAAAAT 
GGCACCAGCT TCCATGGCTG TATCCGGAAC CTTTACATTA ACAGTGAGCT GCAGGACTTC 
CGGAAAATGC CTATGCAAAC CGGAATTCTG CCTGGCTGTG AACCATGCCA CAAGAAAGTA 
TGTGCCCATG GCATGTGCCA GCCCAGCAGC CAATCAGGCT TCACCTGTGA ATGTGAGGAA 
GGGTGGATGG GGCCCCTCTG TGACCAGAGA ACCAATGATC CCTGCCTCGG AAACAAATGT 
GTGCATGGGA CCTGCCTGCC CATCAATGCC TTCTCCTATA GTTGCAAGTG CCTGGAGGGC 
CATGGCGGTG TCCTCTGTGA TGAAGAAGAA GATCTCTTTA ACCCCTGCCA GATGATCAAG 
TGCAAGCATG GGAAGTGCAG GCTTTCTGGA GTGGGCCAGC CCTATTGTGA ATGCAACAGT 
GGATTCACCG GGGACAGCTG TGATAGAGAA ATTTCTTGTC GAGGGGAACG GATAAGGGAC 
TATTACCAGA AGCAGCAGGG TTACGCTGCC TGTCAAACAA CTAAGAAAGT ATCTCGCTTG 
GAATGCAGAG GCGGGTGCGC TGGAGGCCAG TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG 
AAATACTCTT TCGAATGCAC AGATGGCTCC TCATTTGTGG ACGAGGTTGA GAAAGTGGTG 
AAGTGCGGCT GCGCGAGATG TGCCTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Slit2
Entrez Gene ID	20563
Full Name	slit guidance ligand 2
Synonyms	Drad-1,E030015M03Rik,E130320P19Rik,Slil3,b2b1200.1Clo,mKIAA4141,slit-2
General protein information	Preferred Names slit guidance ligand 2 Names slit homolog 2 protein downregulated during adipocyte differentiation-1 neurogenic extracellular slit protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B3
Summary	The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

	SLIT2	XP_002808451.1
Canis lupus familiaris (dog)	SLIT2	XP_005618806.1
Danio rerio (zebrafish)	slit2	NP_571810.1
Xenopus tropicalis (tropical clawed frog)	slit2	XP_004911285.1
Homo sapiens (human)	SLIT2	NP_004778.1
Pan troglodytes (chimpanzee)	SLIT2	XP_003950343.1
Gallus gallus (chicken)	SLIT2	NP_001254004.1
Caenorhabditis elegans (roundworm)	slt-1	NP_510437.2
Bos taurus (cattle)	SLIT2	NP_001178445.2
Mus musculus (house mouse)	Slit2	NP_848919.3
Rattus norvegicus (Norway rat)	Slit2	NP_072154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002793	XP_312121.5
Drosophila melanogaster (fruit fly)	sli	NP_001261017.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Slit2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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