SLIT2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLIT2
Entrez Gene ID	534164
Full Name	slit guidance ligand 2
General protein information	Preferred Names slit guidance ligand 2 Names slit homolog 2 protein slit homolog 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005207790.3	XP_005207847.1	slit homolog 2 protein isoform X1
XM_005207792.4	XP_005207849.1	slit homolog 2 protein isoform X2
XM_005207791.3	XP_005207848.1	slit homolog 2 protein isoform X2
NM_001191516.2	NP_001178445.2	slit homolog 2 protein precursor
XM_005207790.4	XP_005207847.1	slit homolog 2 protein isoform X1
XM_005207792.3	XP_005207849.1	slit homolog 2 protein isoform X3

Pathways from BioSystems

Homologies

	SLIT2	XP_002808451.1
Canis lupus familiaris (dog)	SLIT2	XP_005618806.1
Danio rerio (zebrafish)	slit2	NP_571810.1
Xenopus tropicalis (tropical clawed frog)	slit2	XP_004911285.1
Homo sapiens (human)	SLIT2	NP_004778.1
Pan troglodytes (chimpanzee)	SLIT2	XP_003950343.1
Gallus gallus (chicken)	SLIT2	NP_001254004.1
Caenorhabditis elegans (roundworm)	slt-1	NP_510437.2
Bos taurus (cattle)	SLIT2	NP_001178445.2
Mus musculus (house mouse)	Slit2	NP_848919.3
Rattus norvegicus (Norway rat)	Slit2	NP_072154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002793	XP_312121.5
Drosophila melanogaster (fruit fly)	sli	NP_001261017.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SLIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39440	NM_001191516.2 Latest version!	Bos taurus slit guidance ligand 2 (SLIT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
OBa20297	XM_005207790.4 Latest version!	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
OBa20298	XM_005207791.3 Latest version!	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
OBa20299	XM_005207792.4 Latest version!	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
View Old Accession Versions >>
OBa20297	XM_005207790.3	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
OBa20299	XM_005207792.3	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLIT2 ( NM_001191516.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178445.2 Bos taurus slit guidance ligand 2 (SLIT2), mRNA.

Price & Availability↑

CloneID	OBa39440
Clone ID Related Accession (Same CDS sequence)	NM_001191516.2
Accession Version	NM_001191516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Bos taurus(cattle)
Product	slit homolog 2 protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000006.1. On Jan 12, 2012 this sequence version replaced NM_001191516.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145437 [ECO:0000348] ##Evidence-Data-END##

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGCAGTG TTTTGCACTG CCCAGCTGCT 
TGTACCTGTA GCAACAACAT CGTAGATTGC CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AATCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATCG ACCTGAGCAA TAATCAGATC 
TCTGAGCTAG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGTTA 
CTATTACTGA ATGCCAACAA GATAAACTGC CTCCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAACC TTCTCTCCTT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTCC GGGCCATTCA AACCATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA TTATCTCCAT ACCAACCCAA TCGAGACCAG TGGTGCCCGC 
TGCACCAGTC CCCGGCGACT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGGGACT GCTTTGCCGA TTTGGCTTGC CCTGAAAAGT GCCGCTGCGA AGGGACCACA 
GTAGACTGCT CCAATCAAAA ACTCACCAAA ATCCCAGATC ACATTCCCCA GTACACTGCA 
GAGCTGCGCC TCAACAATAA TGAATTTACA GTGTTGGAAG CTACCGGGAT CTTCAAGAAA 
CTTCCTCAGT TACGTAAAAT AAACTTTAGC AACAATAAGA TCACAGACAT TGAAGAGGGA 
GCGTTTGAAG GAGCATCTGG TGTGAATGAA ATACTTCTCA CGAGTAATCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGCTTGGAA AGCCTCAAGA CTTTGATGTT GAGAAGTAAT 
CGCATAAGCT GTGTAGGGAA TGACAGTTTC ATAGGACTCA GCTCTGTGCG TTTGCTTTCT 
TTATATGATA ATCAGATTAC TACCATTGCA CCAGGAGCTT TTGATACTCT CCATTCTTTA 
TCTACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GCTACCTGGC TTGGTTGGGA 
GAATGGCTTA GGAAGAAAAG AATTGTAACA GGAAATCCTC GATGTCAGAA ACCCTATTTC 
CTCAAAGAAA TCCCCATCCA GGATGTGGCC ATTCAAGACT TCACTTGTGA TGATGGAAAT 
GATGACAATA GCTGTTCCCC ACTCTCTCGC TGTCCTGCCG AGTGTACCTG CTTGGACACA 
GTGGTTCGAT GTAGCAACAA AGCCTTGAAG GTCTTGCCCA AAGGAATTCC AAGAGATGTC 
ACTGAATTGT ATCTGGATGG GAACCAGTTT ACCTTGGTTC CTAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTCTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACTTTA ATTCTTAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTGAAGTCT CTTCGGTTAC TTTCTTTACA TGGAAACGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAAT GATCTTGCTG CATTATCACA CCTAGCAATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTAAAGTCG 
GAATACAAAG AGCCGGGAAT TGCTCGCTGT GCTGGTCCTG GAGAAATGGC AGATAAACTA 
CTTCTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATGT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCAGTTGACT TTTATCGCTG CACCTGTCCA TATGGTTTCA AGGGGCAGGA TTGTGATGTT 
CCAATTCATG CGTGCATCAG CAACCCATGT AAACATGGAG GAACTTGCCA CTTAAAAGAA 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCTGATGGAT TTGAAGGAGA AAATTGTGAA 
ATCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACATG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGCCCACCT GAGTACACAG GAGAGTTGTG TGAGGAGAAA 
CTGGACTTCT GTGCCCAGGA CTTGAACCCC TGCCAGCATG ACTCCAAGTG CATCCTGACG 
CCAAAGGGAT ACAAATGTGA CTGCACTCCA GGATACATAG GCGAACATTG TGACATTGAC 
TTCGATGACT GCCAAGATAA CAAGTGTAAG AACGGAGCCC ACTGCACCGA TGCAGTGAAC 
GGTTACACAT GCACCTGTCC TGAAGGCTAC AGTGGCTTGT TTTGTGAATT TTCTCCACCT 
ATGGTTCTCC CTCGTACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCTCAATGC 
ATCATCAGGA TCAATGAGCC AATATGCCAG TGTTTGCCTG GCTACCAGGG AGAAAAGTGT 
GAAAAACTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCTT ATCTTCAGAT CCCTTCCGCC 
AAGGTCCGGC CTCAAACAAA CATCACTCTT CAGATCGCCA CAGATGAAGA CAGTGGAATC 
CTCCTGTATA AGGGTGATAA AGACCATATT GCTGTAGAAC TCTACCGAGG ACGTGTTCGT 
GCCAGCTATG ACACCGGCTC CCACCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GACGGAAATT TTCACATTGT GGAACTACTT GCCCTGGATC AAAGTCTCTC CCTCTCAGTG 
GATGGAGGGA GCCCCAAAAT CATTACCAAC TTGTCAAAAC AGTCCACTCT GAATTTTGAC 
TCCCCACTCT ATGTTGGAGG CATGCCCGGG AAGAACAACG TGGCCGCAGC TCTGCGCCAG 
GCCCCTGGGC AGAATGGCAC CAGCTTCCAC GGTTGCATCC GGAACCTTTA CATCAACAGC 
GAACTTCAGG ACTTCCGGAA GGTGCCCATG CAGACCGGCA TCCTGCCTGG CTGTGAACCA 
TGCCACAAGA AGGTGTGTGC CCACGGCACA TGCCAGCCCA GCAGCCAGGC CGGCTTCACC 
TGCGAGTGCG AGGAAGGATG GACAGGGCCC CTCTGTGATC AGAGGACCAA TGACCCCTGT 
CTTGGAAATA AATGCGTCCA CGGCACCTGC CTGCCCATCA ATGCGTTCTC CTACAGCTGC 
AAATGCCTAG AGGGCCATGG GGGCGTCCTC TGTGATGAAG AGGAGGATCT GTTTAACCCA 
TGCCAGGCGA TCAAGTGCAA GCATGGGAAA TGCAGGCTCT CAGGACTGGG GCAGCCCTAC 
TGTGAATGCA GCAGTGGATA CACCGGGGAC AGCTGTGATC GAGAAATCTC TTGTCGAGGG 
GAACGGATAA GAGATTATTA CCAAAAGCAG CAGGGCTACG CCGCTTGCCA GACGACCAAG 
AAGGTGTCTC GGTTGGAATG CAGAGGGGGC TGTGCAGGCG GGCAGTGCTG CGGACCTCTG 
AGGAGCAAGA GAAGGAAATA CTCTTTCGAA TGCACTGATG GGTCCTCGTT TGTGGACGAG 
GTGGAGAAGG TGGTAAAGTG TGGCTGTACC CGCTGCGCTT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178445.2
CDS	320..4924
Translation	MHGVGWQTLSLSLGLVLAILNEVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQITTIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPAECTCLDTVVRCSNKALKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLAALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEKDGFWCICADGFEGENCEINVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGYKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYQGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGKNNVAAALRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS

Target ORF information:

RefSeq Version	NM_001191516.2
Organism	Bos taurus(cattle)
Definition	Bos taurus slit guidance ligand 2 (SLIT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191516.2

ORF Insert Sequence:

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGCAGTG TTTTGCACTG CCCAGCTGCT 
TGTACCTGTA GCAACAACAT CGTAGATTGC CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AATCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATCG ACCTGAGCAA TAATCAGATC 
TCTGAGCTAG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGTTA 
CTATTACTGA ATGCCAACAA GATAAACTGC CTCCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAACC TTCTCTCCTT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTCC GGGCCATTCA AACCATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA TTATCTCCAT ACCAACCCAA TCGAGACCAG TGGTGCCCGC 
TGCACCAGTC CCCGGCGACT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG CTAAAGAACA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA 
AGTGGGGACT GCTTTGCCGA TTTGGCTTGC CCTGAAAAGT GCCGCTGCGA AGGGACCACA 
GTAGACTGCT CCAATCAAAA ACTCACCAAA ATCCCAGATC ACATTCCCCA GTACACTGCA 
GAGCTGCGCC TCAACAATAA TGAATTTACA GTGTTGGAAG CTACCGGGAT CTTCAAGAAA 
CTTCCTCAGT TACGTAAAAT AAACTTTAGC AACAATAAGA TCACAGACAT TGAAGAGGGA 
GCGTTTGAAG GAGCATCTGG TGTGAATGAA ATACTTCTCA CGAGTAATCG TTTGGAAAAT 
GTTCAGCATA AGATGTTCAA GGGCTTGGAA AGCCTCAAGA CTTTGATGTT GAGAAGTAAT 
CGCATAAGCT GTGTAGGGAA TGACAGTTTC ATAGGACTCA GCTCTGTGCG TTTGCTTTCT 
TTATATGATA ATCAGATTAC TACCATTGCA CCAGGAGCTT TTGATACTCT CCATTCTTTA 
TCTACTCTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GCTACCTGGC TTGGTTGGGA 
GAATGGCTTA GGAAGAAAAG AATTGTAACA GGAAATCCTC GATGTCAGAA ACCCTATTTC 
CTCAAAGAAA TCCCCATCCA GGATGTGGCC ATTCAAGACT TCACTTGTGA TGATGGAAAT 
GATGACAATA GCTGTTCCCC ACTCTCTCGC TGTCCTGCCG AGTGTACCTG CTTGGACACA 
GTGGTTCGAT GTAGCAACAA AGCCTTGAAG GTCTTGCCCA AAGGAATTCC AAGAGATGTC 
ACTGAATTGT ATCTGGATGG GAACCAGTTT ACCTTGGTTC CTAAGGAACT CTCTAACTAC 
AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTCTC TAATCAGAGC 
TTCAGCAACA TGACCCAGCT CCTCACTTTA ATTCTTAGTT ACAACCGTTT GAGATGTATT 
CCTCCTCGAA CCTTCGATGG ACTGAAGTCT CTTCGGTTAC TTTCTTTACA TGGAAACGAC 
ATTTCTGTTG TGCCTGAAGG TGCTTTCAAT GATCTTGCTG CATTATCACA CCTAGCAATT 
GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTAAAGTCG 
GAATACAAAG AGCCGGGAAT TGCTCGCTGT GCTGGTCCTG GAGAAATGGC AGATAAACTA 
CTTCTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATGT CAATATTCTA 
GCTAAATGTA ATCCCTGCTT ATCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC 
CCAGTTGACT TTTATCGCTG CACCTGTCCA TATGGTTTCA AGGGGCAGGA TTGTGATGTT 
CCAATTCATG CGTGCATCAG CAACCCATGT AAACATGGAG GAACTTGCCA CTTAAAAGAA 
GGAGAAAAAG ATGGATTCTG GTGTATTTGT GCTGATGGAT TTGAAGGAGA AAATTGTGAA 
ATCAATGTTG ATGACTGTGA AGATAATGAC TGTGAAAATA ACTCTACATG TGTCGATGGA 
ATTAATAACT ACACATGCCT TTGCCCACCT GAGTACACAG GAGAGTTGTG TGAGGAGAAA 
CTGGACTTCT GTGCCCAGGA CTTGAACCCC TGCCAGCATG ACTCCAAGTG CATCCTGACG 
CCAAAGGGAT ACAAATGTGA CTGCACTCCA GGATACATAG GCGAACATTG TGACATTGAC 
TTCGATGACT GCCAAGATAA CAAGTGTAAG AACGGAGCCC ACTGCACCGA TGCAGTGAAC 
GGTTACACAT GCACCTGTCC TGAAGGCTAC AGTGGCTTGT TTTGTGAATT TTCTCCACCT 
ATGGTTCTCC CTCGTACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCTCAATGC 
ATCATCAGGA TCAATGAGCC AATATGCCAG TGTTTGCCTG GCTACCAGGG AGAAAAGTGT 
GAAAAACTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCTT ATCTTCAGAT CCCTTCCGCC 
AAGGTCCGGC CTCAAACAAA CATCACTCTT CAGATCGCCA CAGATGAAGA CAGTGGAATC 
CTCCTGTATA AGGGTGATAA AGACCATATT GCTGTAGAAC TCTACCGAGG ACGTGTTCGT 
GCCAGCTATG ACACCGGCTC CCACCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT 
GACGGAAATT TTCACATTGT GGAACTACTT GCCCTGGATC AAAGTCTCTC CCTCTCAGTG 
GATGGAGGGA GCCCCAAAAT CATTACCAAC TTGTCAAAAC AGTCCACTCT GAATTTTGAC 
TCCCCACTCT ATGTTGGAGG CATGCCCGGG AAGAACAACG TGGCCGCAGC TCTGCGCCAG 
GCCCCTGGGC AGAATGGCAC CAGCTTCCAC GGTTGCATCC GGAACCTTTA CATCAACAGC 
GAACTTCAGG ACTTCCGGAA GGTGCCCATG CAGACCGGCA TCCTGCCTGG CTGTGAACCA 
TGCCACAAGA AGGTGTGTGC CCACGGCACA TGCCAGCCCA GCAGCCAGGC CGGCTTCACC 
TGCGAGTGCG AGGAAGGATG GACAGGGCCC CTCTGTGATC AGAGGACCAA TGACCCCTGT 
CTTGGAAATA AATGCGTCCA CGGCACCTGC CTGCCCATCA ATGCGTTCTC CTACAGCTGC 
AAATGCCTAG AGGGCCATGG GGGCGTCCTC TGTGATGAAG AGGAGGATCT GTTTAACCCA 
TGCCAGGCGA TCAAGTGCAA GCATGGGAAA TGCAGGCTCT CAGGACTGGG GCAGCCCTAC 
TGTGAATGCA GCAGTGGATA CACCGGGGAC AGCTGTGATC GAGAAATCTC TTGTCGAGGG 
GAACGGATAA GAGATTATTA CCAAAAGCAG CAGGGCTACG CCGCTTGCCA GACGACCAAG 
AAGGTGTCTC GGTTGGAATG CAGAGGGGGC TGTGCAGGCG GGCAGTGCTG CGGACCTCTG 
AGGAGCAAGA GAAGGAAATA CTCTTTCGAA TGCACTGATG GGTCCTCGTT TGTGGACGAG 
GTGGAGAAGG TGGTAAAGTG TGGCTGTACC CGCTGCGCTT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_005207790.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207847.1 Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20297
Clone ID Related Accession (Same CDS sequence)	XM_005207790.3 , XM_005207790.4
Accession Version	XM_005207790.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103970.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGGGACTGC 
TTTGCCGATT TGGCTTGCCC TGAAAAGTGC CGCTGCGAAG GGACCACAGT AGACTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGATCAC ATTCCCCAGT ACACTGCAGA GCTGCGCCTC 
AACAATAATG AATTTACAGT GTTGGAAGCT ACCGGGATCT TCAAGAAACT TCCTCAGTTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGACATTG AAGAGGGAGC GTTTGAAGGA 
GCATCTGGTG TGAATGAAAT ACTTCTCACG AGTAATCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GCTTGGAAAG CCTCAAGACT TTGATGTTGA GAAGTAATCG CATAAGCTGT 
GTAGGGAATG ACAGTTTCAT AGGACTCAGC TCTGTGCGTT TGCTTTCTTT ATATGATAAT 
CAGATTACTA CCATTGCACC AGGAGCTTTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA ATGGCTTAGG 
AAGAAAAGAA TTGTAACAGG AAATCCTCGA TGTCAGAAAC CCTATTTCCT CAAAGAAATC 
CCCATCCAGG ATGTGGCCAT TCAAGACTTC ACTTGTGATG ATGGAAATGA TGACAATAGC 
TGTTCCCCAC TCTCTCGCTG TCCTGCCGAG TGTACCTGCT TGGACACAGT GGTTCGATGT 
AGCAACAAAG CCTTGAAGGT CTTGCCCAAA GGAATTCCAA GAGATGTCAC TGAATTGTAT 
CTGGATGGGA ACCAGTTTAC CTTGGTTCCT AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACCCTCTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACTTTAAT TCTTAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TGAAGTCTCT TCGGTTACTT TCTTTACATG GAAACGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTGCTGCA TTATCACACC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TAAAGTCGGA ATACAAAGAG 
CCGGGAATTG CTCGCTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTACT TCTCACAACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC AGTTGACTTT 
TATCGCTGCA CCTGTCCATA TGGTTTCAAG GGGCAGGATT GTGATGTTCC AATTCATGCG 
TGCATCAGCA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAAGAAGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAAT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACATGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTACACAGGA GAGTTGTGTG AGGAGAAACT GGACTTCTGT 
GCCCAGGACT TGAACCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACGCC AAAGGGATAC 
AAATGTGACT GCACTCCAGG ATACATAGGC GAACATTGTG ACATTGACTT CGATGACTGC 
CAAGATAACA AGTGTAAGAA CGGAGCCCAC TGCACCGATG CAGTGAACGG TTACACATGC 
ACCTGTCCTG AAGGCTACAG TGGCTTGTTT TGTGAATTTT CTCCACCTAT GGTTCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAATGCAT CATCAGGATC 
AATGAGCCAA TATGCCAGTG TTTGCCTGGC TACCAGGGAG AAAAGTGTGA AAAACTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCTTAT CTTCAGATCC CTTCCGCCAA GGTCCGGCCT 
CAAACAAACA TCACTCTTCA GATCGCCACA GATGAAGACA GTGGAATCCT CCTGTATAAG 
GGTGATAAAG ACCATATTGC TGTAGAACTC TACCGAGGAC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCCC ACCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA CGGAAATTTT 
CACATTGTGG AACTACTTGC CCTGGATCAA AGTCTCTCCC TCTCAGTGGA TGGAGGGAGC 
CCCAAAATCA TTACCAACTT GTCAAAACAG TCCACTCTGA ATTTTGACTC CCCACTCTAT 
GTTGGAGGCA TGCCCGGGAA GAACAACGTG GCCGCAGCTC TGCGCCAGGC CCCTGGGCAG 
AATGGCACCA GCTTCCACGG TTGCATCCGG AACCTTTACA TCAACAGCGA ACTTCAGGAC 
TTCCGGAAGG TGCCCATGCA GACCGGCATC CTGCCTGGCT GTGAACCATG CCACAAGAAG 
GTGTGTGCCC ACGGCACATG CCAGCCCAGC AGCCAGGCCG GCTTCACCTG CGAGTGCGAG 
GAAGGATGGA CAGGGCCCCT CTGTGATCAG AGGACCAATG ACCCCTGTCT TGGAAATAAA 
TGCGTCCACG GCACCTGCCT GCCCATCAAT GCGTTCTCCT ACAGCTGCAA ATGCCTAGAG 
GGCCATGGGG GCGTCCTCTG TGATGAAGAG GAGGATCTGT TTAACCCATG CCAGGCGATC 
AAGTGCAAGC ATGGGAAATG CAGGCTCTCA GGACTGGGGC AGCCCTACTG TGAATGCAGC 
AGTGGATACA CCGGGGACAG CTGTGATCGA GAAATCTCTT GTCGAGGGGA ACGGATAAGA 
GATTATTACC AAAAGCAGCA GGGCTACGCC GCTTGCCAGA CGACCAAGAA GGTGTCTCGG 
TTGGAATGCA GAGGGGGCTG TGCAGGCGGG CAGTGCTGCG GACCTCTGAG GAGCAAGAGA 
AGGAAATACT CTTTCGAATG CACTGATGGG TCCTCGTTTG TGGACGAGGT GGAGAAGGTG 
GTAAAGTGTG GCTGTACCCG CTGCGCTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207847.1
CDS	616..5208
Translation	MHGVGWQTLSLSLGLVLAILNEVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQITTIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPAECTCLDTVVRCSNKALKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLAALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEKDGFWCICADGFEGENCEINVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGYKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYQGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGKNNVAAALRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS

Target ORF information:

RefSeq Version	XM_005207790.3
Organism	Bos taurus(cattle)
Definition	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207790.3

ORF Insert Sequence:

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGGGACTGC 
TTTGCCGATT TGGCTTGCCC TGAAAAGTGC CGCTGCGAAG GGACCACAGT AGACTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGATCAC ATTCCCCAGT ACACTGCAGA GCTGCGCCTC 
AACAATAATG AATTTACAGT GTTGGAAGCT ACCGGGATCT TCAAGAAACT TCCTCAGTTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGACATTG AAGAGGGAGC GTTTGAAGGA 
GCATCTGGTG TGAATGAAAT ACTTCTCACG AGTAATCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GCTTGGAAAG CCTCAAGACT TTGATGTTGA GAAGTAATCG CATAAGCTGT 
GTAGGGAATG ACAGTTTCAT AGGACTCAGC TCTGTGCGTT TGCTTTCTTT ATATGATAAT 
CAGATTACTA CCATTGCACC AGGAGCTTTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA ATGGCTTAGG 
AAGAAAAGAA TTGTAACAGG AAATCCTCGA TGTCAGAAAC CCTATTTCCT CAAAGAAATC 
CCCATCCAGG ATGTGGCCAT TCAAGACTTC ACTTGTGATG ATGGAAATGA TGACAATAGC 
TGTTCCCCAC TCTCTCGCTG TCCTGCCGAG TGTACCTGCT TGGACACAGT GGTTCGATGT 
AGCAACAAAG CCTTGAAGGT CTTGCCCAAA GGAATTCCAA GAGATGTCAC TGAATTGTAT 
CTGGATGGGA ACCAGTTTAC CTTGGTTCCT AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACCCTCTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACTTTAAT TCTTAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TGAAGTCTCT TCGGTTACTT TCTTTACATG GAAACGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTGCTGCA TTATCACACC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TAAAGTCGGA ATACAAAGAG 
CCGGGAATTG CTCGCTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTACT TCTCACAACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC AGTTGACTTT 
TATCGCTGCA CCTGTCCATA TGGTTTCAAG GGGCAGGATT GTGATGTTCC AATTCATGCG 
TGCATCAGCA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAAGAAGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAAT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACATGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTACACAGGA GAGTTGTGTG AGGAGAAACT GGACTTCTGT 
GCCCAGGACT TGAACCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACGCC AAAGGGATAC 
AAATGTGACT GCACTCCAGG ATACATAGGC GAACATTGTG ACATTGACTT CGATGACTGC 
CAAGATAACA AGTGTAAGAA CGGAGCCCAC TGCACCGATG CAGTGAACGG TTACACATGC 
ACCTGTCCTG AAGGCTACAG TGGCTTGTTT TGTGAATTTT CTCCACCTAT GGTTCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAATGCAT CATCAGGATC 
AATGAGCCAA TATGCCAGTG TTTGCCTGGC TACCAGGGAG AAAAGTGTGA AAAACTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCTTAT CTTCAGATCC CTTCCGCCAA GGTCCGGCCT 
CAAACAAACA TCACTCTTCA GATCGCCACA GATGAAGACA GTGGAATCCT CCTGTATAAG 
GGTGATAAAG ACCATATTGC TGTAGAACTC TACCGAGGAC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCCC ACCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA CGGAAATTTT 
CACATTGTGG AACTACTTGC CCTGGATCAA AGTCTCTCCC TCTCAGTGGA TGGAGGGAGC 
CCCAAAATCA TTACCAACTT GTCAAAACAG TCCACTCTGA ATTTTGACTC CCCACTCTAT 
GTTGGAGGCA TGCCCGGGAA GAACAACGTG GCCGCAGCTC TGCGCCAGGC CCCTGGGCAG 
AATGGCACCA GCTTCCACGG TTGCATCCGG AACCTTTACA TCAACAGCGA ACTTCAGGAC 
TTCCGGAAGG TGCCCATGCA GACCGGCATC CTGCCTGGCT GTGAACCATG CCACAAGAAG 
GTGTGTGCCC ACGGCACATG CCAGCCCAGC AGCCAGGCCG GCTTCACCTG CGAGTGCGAG 
GAAGGATGGA CAGGGCCCCT CTGTGATCAG AGGACCAATG ACCCCTGTCT TGGAAATAAA 
TGCGTCCACG GCACCTGCCT GCCCATCAAT GCGTTCTCCT ACAGCTGCAA ATGCCTAGAG 
GGCCATGGGG GCGTCCTCTG TGATGAAGAG GAGGATCTGT TTAACCCATG CCAGGCGATC 
AAGTGCAAGC ATGGGAAATG CAGGCTCTCA GGACTGGGGC AGCCCTACTG TGAATGCAGC 
AGTGGATACA CCGGGGACAG CTGTGATCGA GAAATCTCTT GTCGAGGGGA ACGGATAAGA 
GATTATTACC AAAAGCAGCA GGGCTACGCC GCTTGCCAGA CGACCAAGAA GGTGTCTCGG 
TTGGAATGCA GAGGGGGCTG TGCAGGCGGG CAGTGCTGCG GACCTCTGAG GAGCAAGAGA 
AGGAAATACT CTTTCGAATG CACTGATGGG TCCTCGTTTG TGGACGAGGT GGAGAAGGTG 
GTAAAGTGTG GCTGTACCCG CTGCGCTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_005207790.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207847.1 Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

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CloneID	OBa20297
Clone ID Related Accession (Same CDS sequence)	XM_005207790.3 , XM_005207790.4
Accession Version	XM_005207790.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	slit homolog 2 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207790.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGGGACTGC 
TTTGCCGATT TGGCTTGCCC TGAAAAGTGC CGCTGCGAAG GGACCACAGT AGACTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGATCAC ATTCCCCAGT ACACTGCAGA GCTGCGCCTC 
AACAATAATG AATTTACAGT GTTGGAAGCT ACCGGGATCT TCAAGAAACT TCCTCAGTTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGACATTG AAGAGGGAGC GTTTGAAGGA 
GCATCTGGTG TGAATGAAAT ACTTCTCACG AGTAATCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GCTTGGAAAG CCTCAAGACT TTGATGTTGA GAAGTAATCG CATAAGCTGT 
GTAGGGAATG ACAGTTTCAT AGGACTCAGC TCTGTGCGTT TGCTTTCTTT ATATGATAAT 
CAGATTACTA CCATTGCACC AGGAGCTTTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA ATGGCTTAGG 
AAGAAAAGAA TTGTAACAGG AAATCCTCGA TGTCAGAAAC CCTATTTCCT CAAAGAAATC 
CCCATCCAGG ATGTGGCCAT TCAAGACTTC ACTTGTGATG ATGGAAATGA TGACAATAGC 
TGTTCCCCAC TCTCTCGCTG TCCTGCCGAG TGTACCTGCT TGGACACAGT GGTTCGATGT 
AGCAACAAAG CCTTGAAGGT CTTGCCCAAA GGAATTCCAA GAGATGTCAC TGAATTGTAT 
CTGGATGGGA ACCAGTTTAC CTTGGTTCCT AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACCCTCTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACTTTAAT TCTTAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TGAAGTCTCT TCGGTTACTT TCTTTACATG GAAACGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTGCTGCA TTATCACACC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TAAAGTCGGA ATACAAAGAG 
CCGGGAATTG CTCGCTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTACT TCTCACAACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC AGTTGACTTT 
TATCGCTGCA CCTGTCCATA TGGTTTCAAG GGGCAGGATT GTGATGTTCC AATTCATGCG 
TGCATCAGCA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAAGAAGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAAT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACATGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTACACAGGA GAGTTGTGTG AGGAGAAACT GGACTTCTGT 
GCCCAGGACT TGAACCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACGCC AAAGGGATAC 
AAATGTGACT GCACTCCAGG ATACATAGGC GAACATTGTG ACATTGACTT CGATGACTGC 
CAAGATAACA AGTGTAAGAA CGGAGCCCAC TGCACCGATG CAGTGAACGG TTACACATGC 
ACCTGTCCTG AAGGCTACAG TGGCTTGTTT TGTGAATTTT CTCCACCTAT GGTTCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAATGCAT CATCAGGATC 
AATGAGCCAA TATGCCAGTG TTTGCCTGGC TACCAGGGAG AAAAGTGTGA AAAACTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCTTAT CTTCAGATCC CTTCCGCCAA GGTCCGGCCT 
CAAACAAACA TCACTCTTCA GATCGCCACA GATGAAGACA GTGGAATCCT CCTGTATAAG 
GGTGATAAAG ACCATATTGC TGTAGAACTC TACCGAGGAC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCCC ACCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA CGGAAATTTT 
CACATTGTGG AACTACTTGC CCTGGATCAA AGTCTCTCCC TCTCAGTGGA TGGAGGGAGC 
CCCAAAATCA TTACCAACTT GTCAAAACAG TCCACTCTGA ATTTTGACTC CCCACTCTAT 
GTTGGAGGCA TGCCCGGGAA GAACAACGTG GCCGCAGCTC TGCGCCAGGC CCCTGGGCAG 
AATGGCACCA GCTTCCACGG TTGCATCCGG AACCTTTACA TCAACAGCGA ACTTCAGGAC 
TTCCGGAAGG TGCCCATGCA GACCGGCATC CTGCCTGGCT GTGAACCATG CCACAAGAAG 
GTGTGTGCCC ACGGCACATG CCAGCCCAGC AGCCAGGCCG GCTTCACCTG CGAGTGCGAG 
GAAGGATGGA CAGGGCCCCT CTGTGATCAG AGGACCAATG ACCCCTGTCT TGGAAATAAA 
TGCGTCCACG GCACCTGCCT GCCCATCAAT GCGTTCTCCT ACAGCTGCAA ATGCCTAGAG 
GGCCATGGGG GCGTCCTCTG TGATGAAGAG GAGGATCTGT TTAACCCATG CCAGGCGATC 
AAGTGCAAGC ATGGGAAATG CAGGCTCTCA GGACTGGGGC AGCCCTACTG TGAATGCAGC 
AGTGGATACA CCGGGGACAG CTGTGATCGA GAAATCTCTT GTCGAGGGGA ACGGATAAGA 
GATTATTACC AAAAGCAGCA GGGCTACGCC GCTTGCCAGA CGACCAAGAA GGTGTCTCGG 
TTGGAATGCA GAGGGGGCTG TGCAGGCGGG CAGTGCTGCG GACCTCTGAG GAGCAAGAGA 
AGGAAATACT CTTTCGAATG CACTGATGGG TCCTCGTTTG TGGACGAGGT GGAGAAGGTG 
GTAAAGTGTG GCTGTACCCG CTGCGCTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207847.1
CDS	621..5213
Translation	MHGVGWQTLSLSLGLVLAILNEVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQITTIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPAECTCLDTVVRCSNKALKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLAALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEKDGFWCICADGFEGENCEINVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGYKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYQGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGKNNVAAALRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS

Target ORF information:

RefSeq Version	XM_005207790.4
Organism	Bos taurus(cattle)
Definition	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207790.4

ORF Insert Sequence:

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT 
AAAGAACAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGGGACTGC 
TTTGCCGATT TGGCTTGCCC TGAAAAGTGC CGCTGCGAAG GGACCACAGT AGACTGCTCC 
AATCAAAAAC TCACCAAAAT CCCAGATCAC ATTCCCCAGT ACACTGCAGA GCTGCGCCTC 
AACAATAATG AATTTACAGT GTTGGAAGCT ACCGGGATCT TCAAGAAACT TCCTCAGTTA 
CGTAAAATAA ACTTTAGCAA CAATAAGATC ACAGACATTG AAGAGGGAGC GTTTGAAGGA 
GCATCTGGTG TGAATGAAAT ACTTCTCACG AGTAATCGTT TGGAAAATGT TCAGCATAAG 
ATGTTCAAGG GCTTGGAAAG CCTCAAGACT TTGATGTTGA GAAGTAATCG CATAAGCTGT 
GTAGGGAATG ACAGTTTCAT AGGACTCAGC TCTGTGCGTT TGCTTTCTTT ATATGATAAT 
CAGATTACTA CCATTGCACC AGGAGCTTTT GATACTCTCC ATTCTTTATC TACTCTAAAC 
CTCTTGGCCA ATCCTTTCAA CTGTAACTGC TACCTGGCTT GGTTGGGAGA ATGGCTTAGG 
AAGAAAAGAA TTGTAACAGG AAATCCTCGA TGTCAGAAAC CCTATTTCCT CAAAGAAATC 
CCCATCCAGG ATGTGGCCAT TCAAGACTTC ACTTGTGATG ATGGAAATGA TGACAATAGC 
TGTTCCCCAC TCTCTCGCTG TCCTGCCGAG TGTACCTGCT TGGACACAGT GGTTCGATGT 
AGCAACAAAG CCTTGAAGGT CTTGCCCAAA GGAATTCCAA GAGATGTCAC TGAATTGTAT 
CTGGATGGGA ACCAGTTTAC CTTGGTTCCT AAGGAACTCT CTAACTACAA ACATTTAACA 
CTTATAGACT TAAGTAACAA CAGAATAAGC ACCCTCTCTA ATCAGAGCTT CAGCAACATG 
ACCCAGCTCC TCACTTTAAT TCTTAGTTAC AACCGTTTGA GATGTATTCC TCCTCGAACC 
TTCGATGGAC TGAAGTCTCT TCGGTTACTT TCTTTACATG GAAACGACAT TTCTGTTGTG 
CCTGAAGGTG CTTTCAATGA TCTTGCTGCA TTATCACACC TAGCAATTGG AGCCAACCCT 
CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TAAAGTCGGA ATACAAAGAG 
CCGGGAATTG CTCGCTGTGC TGGTCCTGGA GAAATGGCAG ATAAACTACT TCTCACAACT 
CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATGTCA ATATTCTAGC TAAATGTAAT 
CCCTGCTTAT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC AGTTGACTTT 
TATCGCTGCA CCTGTCCATA TGGTTTCAAG GGGCAGGATT GTGATGTTCC AATTCATGCG 
TGCATCAGCA ACCCATGTAA ACATGGAGGA ACTTGCCACT TAAAAGAAGG AGAAAAAGAT 
GGATTCTGGT GTATTTGTGC TGATGGATTT GAAGGAGAAA ATTGTGAAAT CAATGTTGAT 
GACTGTGAAG ATAATGACTG TGAAAATAAC TCTACATGTG TCGATGGAAT TAATAACTAC 
ACATGCCTTT GCCCACCTGA GTACACAGGA GAGTTGTGTG AGGAGAAACT GGACTTCTGT 
GCCCAGGACT TGAACCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACGCC AAAGGGATAC 
AAATGTGACT GCACTCCAGG ATACATAGGC GAACATTGTG ACATTGACTT CGATGACTGC 
CAAGATAACA AGTGTAAGAA CGGAGCCCAC TGCACCGATG CAGTGAACGG TTACACATGC 
ACCTGTCCTG AAGGCTACAG TGGCTTGTTT TGTGAATTTT CTCCACCTAT GGTTCTCCCT 
CGTACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CTCAATGCAT CATCAGGATC 
AATGAGCCAA TATGCCAGTG TTTGCCTGGC TACCAGGGAG AAAAGTGTGA AAAACTGGTC 
AGTGTGAATT TTGTAAACAA AGAGTCTTAT CTTCAGATCC CTTCCGCCAA GGTCCGGCCT 
CAAACAAACA TCACTCTTCA GATCGCCACA GATGAAGACA GTGGAATCCT CCTGTATAAG 
GGTGATAAAG ACCATATTGC TGTAGAACTC TACCGAGGAC GTGTTCGTGC CAGCTATGAC 
ACCGGCTCCC ACCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA CGGAAATTTT 
CACATTGTGG AACTACTTGC CCTGGATCAA AGTCTCTCCC TCTCAGTGGA TGGAGGGAGC 
CCCAAAATCA TTACCAACTT GTCAAAACAG TCCACTCTGA ATTTTGACTC CCCACTCTAT 
GTTGGAGGCA TGCCCGGGAA GAACAACGTG GCCGCAGCTC TGCGCCAGGC CCCTGGGCAG 
AATGGCACCA GCTTCCACGG TTGCATCCGG AACCTTTACA TCAACAGCGA ACTTCAGGAC 
TTCCGGAAGG TGCCCATGCA GACCGGCATC CTGCCTGGCT GTGAACCATG CCACAAGAAG 
GTGTGTGCCC ACGGCACATG CCAGCCCAGC AGCCAGGCCG GCTTCACCTG CGAGTGCGAG 
GAAGGATGGA CAGGGCCCCT CTGTGATCAG AGGACCAATG ACCCCTGTCT TGGAAATAAA 
TGCGTCCACG GCACCTGCCT GCCCATCAAT GCGTTCTCCT ACAGCTGCAA ATGCCTAGAG 
GGCCATGGGG GCGTCCTCTG TGATGAAGAG GAGGATCTGT TTAACCCATG CCAGGCGATC 
AAGTGCAAGC ATGGGAAATG CAGGCTCTCA GGACTGGGGC AGCCCTACTG TGAATGCAGC 
AGTGGATACA CCGGGGACAG CTGTGATCGA GAAATCTCTT GTCGAGGGGA ACGGATAAGA 
GATTATTACC AAAAGCAGCA GGGCTACGCC GCTTGCCAGA CGACCAAGAA GGTGTCTCGG 
TTGGAATGCA GAGGGGGCTG TGCAGGCGGG CAGTGCTGCG GACCTCTGAG GAGCAAGAGA 
AGGAAATACT CTTTCGAATG CACTGATGGG TCCTCGTTTG TGGACGAGGT GGAGAAGGTG 
GTAAAGTGTG GCTGTACCCG CTGCGCTTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_005207791.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207848.1 Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

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CloneID	OBa20298
Clone ID Related Accession (Same CDS sequence)	XM_005207791.3
Accession Version	XM_005207791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103970.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGCAGTG TTTTGCACTG CCCAGCTGCT 
TGTACCTGTA GCAACAACAT CGTAGATTGC CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AATCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATCG ACCTGAGCAA TAATCAGATC 
TCTGAGCTAG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGTTA 
CTATTACTGA ATGCCAACAA GATAAACTGC CTCCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAACC TTCTCTCCTT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTCC GGGCCATTCA AACCATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA TTATCTCCAT ACCAACCCAA TCGAGACCAG TGGTGCCCGC 
TGCACCAGTC CCCGGCGACT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GGGACTGCTT TGCCGATTTG 
GCTTGCCCTG AAAAGTGCCG CTGCGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAAACTC 
ACCAAAATCC CAGATCACAT TCCCCAGTAC ACTGCAGAGC TGCGCCTCAA CAATAATGAA 
TTTACAGTGT TGGAAGCTAC CGGGATCTTC AAGAAACTTC CTCAGTTACG TAAAATAAAC 
TTTAGCAACA ATAAGATCAC AGACATTGAA GAGGGAGCGT TTGAAGGAGC ATCTGGTGTG 
AATGAAATAC TTCTCACGAG TAATCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGC 
TTGGAAAGCC TCAAGACTTT GATGTTGAGA AGTAATCGCA TAAGCTGTGT AGGGAATGAC 
AGTTTCATAG GACTCAGCTC TGTGCGTTTG CTTTCTTTAT ATGATAATCA GATTACTACC 
ATTGCACCAG GAGCTTTTGA TACTCTCCAT TCTTTATCTA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAAT GGCTTAGGAA GAAAAGAATT 
GTAACAGGAA ATCCTCGATG TCAGAAACCC TATTTCCTCA AAGAAATCCC CATCCAGGAT 
GTGGCCATTC AAGACTTCAC TTGTGATGAT GGAAATGATG ACAATAGCTG TTCCCCACTC 
TCTCGCTGTC CTGCCGAGTG TACCTGCTTG GACACAGTGG TTCGATGTAG CAACAAAGCC 
TTGAAGGTCT TGCCCAAAGG AATTCCAAGA GATGTCACTG AATTGTATCT GGATGGGAAC 
CAGTTTACCT TGGTTCCTAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACA GAATAAGCAC CCTCTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACTTTAATTC TTAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTG 
AAGTCTCTTC GGTTACTTTC TTTACATGGA AACGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAATGATC TTGCTGCATT ATCACACCTA GCAATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTA AAGTCGGAAT ACAAAGAGCC GGGAATTGCT 
CGCTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTACTTC TCACAACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCAG TTGACTTTTA TCGCTGCACC 
TGTCCATATG GTTTCAAGGG GCAGGATTGT GATGTTCCAA TTCATGCGTG CATCAGCAAC 
CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAAGAAGGAG AAAAAGATGG ATTCTGGTGT 
ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAATCA ATGTTGATGA CTGTGAAGAT 
AATGACTGTG AAAATAACTC TACATGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGC 
CCACCTGAGT ACACAGGAGA GTTGTGTGAG GAGAAACTGG ACTTCTGTGC CCAGGACTTG 
AACCCCTGCC AGCATGACTC CAAGTGCATC CTGACGCCAA AGGGATACAA ATGTGACTGC 
ACTCCAGGAT ACATAGGCGA ACATTGTGAC ATTGACTTCG ATGACTGCCA AGATAACAAG 
TGTAAGAACG GAGCCCACTG CACCGATGCA GTGAACGGTT ACACATGCAC CTGTCCTGAA 
GGCTACAGTG GCTTGTTTTG TGAATTTTCT CCACCTATGG TTCTCCCTCG TACCAGCCCC 
TGTGATAATT TTGATTGTCA GAATGGAGCT CAATGCATCA TCAGGATCAA TGAGCCAATA 
TGCCAGTGTT TGCCTGGCTA CCAGGGAGAA AAGTGTGAAA AACTGGTCAG TGTGAATTTT 
GTAAACAAAG AGTCTTATCT TCAGATCCCT TCCGCCAAGG TCCGGCCTCA AACAAACATC 
ACTCTTCAGA TCGCCACAGA TGAAGACAGT GGAATCCTCC TGTATAAGGG TGATAAAGAC 
CATATTGCTG TAGAACTCTA CCGAGGACGT GTTCGTGCCA GCTATGACAC CGGCTCCCAC 
CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGACG GAAATTTTCA CATTGTGGAA 
CTACTTGCCC TGGATCAAAG TCTCTCCCTC TCAGTGGATG GAGGGAGCCC CAAAATCATT 
ACCAACTTGT CAAAACAGTC CACTCTGAAT TTTGACTCCC CACTCTATGT TGGAGGCATG 
CCCGGGAAGA ACAACGTGGC CGCAGCTCTG CGCCAGGCCC CTGGGCAGAA TGGCACCAGC 
TTCCACGGTT GCATCCGGAA CCTTTACATC AACAGCGAAC TTCAGGACTT CCGGAAGGTG 
CCCATGCAGA CCGGCATCCT GCCTGGCTGT GAACCATGCC ACAAGAAGGT GTGTGCCCAC 
GGCACATGCC AGCCCAGCAG CCAGGCCGGC TTCACCTGCG AGTGCGAGGA AGGATGGACA 
GGGCCCCTCT GTGATCAGAG GACCAATGAC CCCTGTCTTG GAAATAAATG CGTCCACGGC 
ACCTGCCTGC CCATCAATGC GTTCTCCTAC AGCTGCAAAT GCCTAGAGGG CCATGGGGGC 
GTCCTCTGTG ATGAAGAGGA GGATCTGTTT AACCCATGCC AGGCGATCAA GTGCAAGCAT 
GGGAAATGCA GGCTCTCAGG ACTGGGGCAG CCCTACTGTG AATGCAGCAG TGGATACACC 
GGGGACAGCT GTGATCGAGA AATCTCTTGT CGAGGGGAAC GGATAAGAGA TTATTACCAA 
AAGCAGCAGG GCTACGCCGC TTGCCAGACG ACCAAGAAGG TGTCTCGGTT GGAATGCAGA 
GGGGGCTGTG CAGGCGGGCA GTGCTGCGGA CCTCTGAGGA GCAAGAGAAG GAAATACTCT 
TTCGAATGCA CTGATGGGTC CTCGTTTGTG GACGAGGTGG AGAAGGTGGT AAAGTGTGGC 
TGTACCCGCT GCGCTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207848.1
CDS	617..5197
Translation	MHGVGWQTLSLSLGLVLAILNEVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQITTIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPAECTCLDTVVRCSNKALKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLAALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEKDGFWCICADGFEGENCEINVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGYKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYQGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGKNNVAAALRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS

Target ORF information:

RefSeq Version	XM_005207791.3
Organism	Bos taurus(cattle)
Definition	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207791.3

ORF Insert Sequence:

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGATGAG 
GAAGAAGGTC ACCAGTCATT TATGGCTCCT TCTTGCAGTG TTTTGCACTG CCCAGCTGCT 
TGTACCTGTA GCAACAACAT CGTAGATTGC CGTGGGAAAG GTCTCACTGA GATCCCCACG 
AATCTGCCAG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCAATCAA GGTCATCCCT 
CCTGGAGCTT TCTCACCATA TAAAAAGCTT AGAAGAATCG ACCTGAGCAA TAATCAGATC 
TCTGAGCTAG CACCAGATGC TTTCCAAGGA CTACGCTCTC TGAATTCACT TGTCCTCTAT 
GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGTTA 
CTATTACTGA ATGCCAACAA GATAAACTGC CTCCGGGTAG ATGCTTTTCA GGATCTGCAC 
AACCTGAACC TTCTCTCCTT ATATGACAAC AAGCTTCAGA CCATCGCCAA GGGGACCTTT 
TCACCTCTCC GGGCCATTCA AACCATGCAT TTGGCCCAGA ACCCCTTTAT TTGTGACTGC 
CATCTCAAGT GGCTGGCGGA TTATCTCCAT ACCAACCCAA TCGAGACCAG TGGTGCCCGC 
TGCACCAGTC CCCGGCGACT GGCAAACAAA AGAATCGGAC AGATCAAAAG CAAGAAATTC 
CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GGGACTGCTT TGCCGATTTG 
GCTTGCCCTG AAAAGTGCCG CTGCGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAAACTC 
ACCAAAATCC CAGATCACAT TCCCCAGTAC ACTGCAGAGC TGCGCCTCAA CAATAATGAA 
TTTACAGTGT TGGAAGCTAC CGGGATCTTC AAGAAACTTC CTCAGTTACG TAAAATAAAC 
TTTAGCAACA ATAAGATCAC AGACATTGAA GAGGGAGCGT TTGAAGGAGC ATCTGGTGTG 
AATGAAATAC TTCTCACGAG TAATCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAGGGC 
TTGGAAAGCC TCAAGACTTT GATGTTGAGA AGTAATCGCA TAAGCTGTGT AGGGAATGAC 
AGTTTCATAG GACTCAGCTC TGTGCGTTTG CTTTCTTTAT ATGATAATCA GATTACTACC 
ATTGCACCAG GAGCTTTTGA TACTCTCCAT TCTTTATCTA CTCTAAACCT CTTGGCCAAT 
CCTTTCAACT GTAACTGCTA CCTGGCTTGG TTGGGAGAAT GGCTTAGGAA GAAAAGAATT 
GTAACAGGAA ATCCTCGATG TCAGAAACCC TATTTCCTCA AAGAAATCCC CATCCAGGAT 
GTGGCCATTC AAGACTTCAC TTGTGATGAT GGAAATGATG ACAATAGCTG TTCCCCACTC 
TCTCGCTGTC CTGCCGAGTG TACCTGCTTG GACACAGTGG TTCGATGTAG CAACAAAGCC 
TTGAAGGTCT TGCCCAAAGG AATTCCAAGA GATGTCACTG AATTGTATCT GGATGGGAAC 
CAGTTTACCT TGGTTCCTAA GGAACTCTCT AACTACAAAC ATTTAACACT TATAGACTTA 
AGTAACAACA GAATAAGCAC CCTCTCTAAT CAGAGCTTCA GCAACATGAC CCAGCTCCTC 
ACTTTAATTC TTAGTTACAA CCGTTTGAGA TGTATTCCTC CTCGAACCTT CGATGGACTG 
AAGTCTCTTC GGTTACTTTC TTTACATGGA AACGACATTT CTGTTGTGCC TGAAGGTGCT 
TTCAATGATC TTGCTGCATT ATCACACCTA GCAATTGGAG CCAACCCTCT TTACTGTGAT 
TGTAACATGC AGTGGTTATC CGACTGGGTA AAGTCGGAAT ACAAAGAGCC GGGAATTGCT 
CGCTGTGCTG GTCCTGGAGA AATGGCAGAT AAACTACTTC TCACAACTCC CTCCAAAAAA 
TTTACATGTC AAGGTCCTGT GGATGTCAAT ATTCTAGCTA AATGTAATCC CTGCTTATCA 
AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCAG TTGACTTTTA TCGCTGCACC 
TGTCCATATG GTTTCAAGGG GCAGGATTGT GATGTTCCAA TTCATGCGTG CATCAGCAAC 
CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAAGAAGGAG AAAAAGATGG ATTCTGGTGT 
ATTTGTGCTG ATGGATTTGA AGGAGAAAAT TGTGAAATCA ATGTTGATGA CTGTGAAGAT 
AATGACTGTG AAAATAACTC TACATGTGTC GATGGAATTA ATAACTACAC ATGCCTTTGC 
CCACCTGAGT ACACAGGAGA GTTGTGTGAG GAGAAACTGG ACTTCTGTGC CCAGGACTTG 
AACCCCTGCC AGCATGACTC CAAGTGCATC CTGACGCCAA AGGGATACAA ATGTGACTGC 
ACTCCAGGAT ACATAGGCGA ACATTGTGAC ATTGACTTCG ATGACTGCCA AGATAACAAG 
TGTAAGAACG GAGCCCACTG CACCGATGCA GTGAACGGTT ACACATGCAC CTGTCCTGAA 
GGCTACAGTG GCTTGTTTTG TGAATTTTCT CCACCTATGG TTCTCCCTCG TACCAGCCCC 
TGTGATAATT TTGATTGTCA GAATGGAGCT CAATGCATCA TCAGGATCAA TGAGCCAATA 
TGCCAGTGTT TGCCTGGCTA CCAGGGAGAA AAGTGTGAAA AACTGGTCAG TGTGAATTTT 
GTAAACAAAG AGTCTTATCT TCAGATCCCT TCCGCCAAGG TCCGGCCTCA AACAAACATC 
ACTCTTCAGA TCGCCACAGA TGAAGACAGT GGAATCCTCC TGTATAAGGG TGATAAAGAC 
CATATTGCTG TAGAACTCTA CCGAGGACGT GTTCGTGCCA GCTATGACAC CGGCTCCCAC 
CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGACG GAAATTTTCA CATTGTGGAA 
CTACTTGCCC TGGATCAAAG TCTCTCCCTC TCAGTGGATG GAGGGAGCCC CAAAATCATT 
ACCAACTTGT CAAAACAGTC CACTCTGAAT TTTGACTCCC CACTCTATGT TGGAGGCATG 
CCCGGGAAGA ACAACGTGGC CGCAGCTCTG CGCCAGGCCC CTGGGCAGAA TGGCACCAGC 
TTCCACGGTT GCATCCGGAA CCTTTACATC AACAGCGAAC TTCAGGACTT CCGGAAGGTG 
CCCATGCAGA CCGGCATCCT GCCTGGCTGT GAACCATGCC ACAAGAAGGT GTGTGCCCAC 
GGCACATGCC AGCCCAGCAG CCAGGCCGGC TTCACCTGCG AGTGCGAGGA AGGATGGACA 
GGGCCCCTCT GTGATCAGAG GACCAATGAC CCCTGTCTTG GAAATAAATG CGTCCACGGC 
ACCTGCCTGC CCATCAATGC GTTCTCCTAC AGCTGCAAAT GCCTAGAGGG CCATGGGGGC 
GTCCTCTGTG ATGAAGAGGA GGATCTGTTT AACCCATGCC AGGCGATCAA GTGCAAGCAT 
GGGAAATGCA GGCTCTCAGG ACTGGGGCAG CCCTACTGTG AATGCAGCAG TGGATACACC 
GGGGACAGCT GTGATCGAGA AATCTCTTGT CGAGGGGAAC GGATAAGAGA TTATTACCAA 
AAGCAGCAGG GCTACGCCGC TTGCCAGACG ACCAAGAAGG TGTCTCGGTT GGAATGCAGA 
GGGGGCTGTG CAGGCGGGCA GTGCTGCGGA CCTCTGAGGA GCAAGAGAAG GAAATACTCT 
TTCGAATGCA CTGATGGGTC CTCGTTTGTG GACGAGGTGG AGAAGGTGGT AAAGTGTGGC 
TGTACCCGCT GCGCTTCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_005207792.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207849.1 Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

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CloneID	OBa20299
Clone ID Related Accession (Same CDS sequence)	XM_005207792.4 , XM_005207792.3
Accession Version	XM_005207792.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	slit homolog 2 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207792.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGG GACTGCTTTG CCGATTTGGC TTGCCCTGAA 
AAGTGCCGCT GCGAAGGGAC CACAGTAGAC TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GATCACATTC CCCAGTACAC TGCAGAGCTG CGCCTCAACA ATAATGAATT TACAGTGTTG 
GAAGCTACCG GGATCTTCAA GAAACTTCCT CAGTTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ACATTGAAGA GGGAGCGTTT GAAGGAGCAT CTGGTGTGAA TGAAATACTT 
CTCACGAGTA ATCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGCTT GGAAAGCCTC 
AAGACTTTGA TGTTGAGAAG TAATCGCATA AGCTGTGTAG GGAATGACAG TTTCATAGGA 
CTCAGCTCTG TGCGTTTGCT TTCTTTATAT GATAATCAGA TTACTACCAT TGCACCAGGA 
GCTTTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTCAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAATGG CTTAGGAAGA AAAGAATTGT AACAGGAAAT 
CCTCGATGTC AGAAACCCTA TTTCCTCAAA GAAATCCCCA TCCAGGATGT GGCCATTCAA 
GACTTCACTT GTGATGATGG AAATGATGAC AATAGCTGTT CCCCACTCTC TCGCTGTCCT 
GCCGAGTGTA CCTGCTTGGA CACAGTGGTT CGATGTAGCA ACAAAGCCTT GAAGGTCTTG 
CCCAAAGGAA TTCCAAGAGA TGTCACTGAA TTGTATCTGG ATGGGAACCA GTTTACCTTG 
GTTCCTAAGG AACTCTCTAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACCC TCTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC TTTAATTCTT 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTGAA GTCTCTTCGG 
TTACTTTCTT TACATGGAAA CGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
GCTGCATTAT CACACCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTAAA GTCGGAATAC AAAGAGCCGG GAATTGCTCG CTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTACTTCTC ACAACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA TCCATGTAAA 
AATGATGGCA CCTGTAACAA TGACCCAGTT GACTTTTATC GCTGCACCTG TCCATATGGT 
TTCAAGGGGC AGGATTGTGA TGTTCCAATT CATGCGTGCA TCAGCAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA AGAAGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAATCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CATGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAC 
ACAGGAGAGT TGTGTGAGGA GAAACTGGAC TTCTGTGCCC AGGACTTGAA CCCCTGCCAG 
CATGACTCCA AGTGCATCCT GACGCCAAAG GGATACAAAT GTGACTGCAC TCCAGGATAC 
ATAGGCGAAC ATTGTGACAT TGACTTCGAT GACTGCCAAG ATAACAAGTG TAAGAACGGA 
GCCCACTGCA CCGATGCAGT GAACGGTTAC ACATGCACCT GTCCTGAAGG CTACAGTGGC 
TTGTTTTGTG AATTTTCTCC ACCTATGGTT CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA ATGCATCATC AGGATCAATG AGCCAATATG CCAGTGTTTG 
CCTGGCTACC AGGGAGAAAA GTGTGAAAAA CTGGTCAGTG TGAATTTTGT AAACAAAGAG 
TCTTATCTTC AGATCCCTTC CGCCAAGGTC CGGCCTCAAA CAAACATCAC TCTTCAGATC 
GCCACAGATG AAGACAGTGG AATCCTCCTG TATAAGGGTG ATAAAGACCA TATTGCTGTA 
GAACTCTACC GAGGACGTGT TCGTGCCAGC TATGACACCG GCTCCCACCC GGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGACGGA AATTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAAAGTC TCTCCCTCTC AGTGGATGGA GGGAGCCCCA AAATCATTAC CAACTTGTCA 
AAACAGTCCA CTCTGAATTT TGACTCCCCA CTCTATGTTG GAGGCATGCC CGGGAAGAAC 
AACGTGGCCG CAGCTCTGCG CCAGGCCCCT GGGCAGAATG GCACCAGCTT CCACGGTTGC 
ATCCGGAACC TTTACATCAA CAGCGAACTT CAGGACTTCC GGAAGGTGCC CATGCAGACC 
GGCATCCTGC CTGGCTGTGA ACCATGCCAC AAGAAGGTGT GTGCCCACGG CACATGCCAG 
CCCAGCAGCC AGGCCGGCTT CACCTGCGAG TGCGAGGAAG GATGGACAGG GCCCCTCTGT 
GATCAGAGGA CCAATGACCC CTGTCTTGGA AATAAATGCG TCCACGGCAC CTGCCTGCCC 
ATCAATGCGT TCTCCTACAG CTGCAAATGC CTAGAGGGCC ATGGGGGCGT CCTCTGTGAT 
GAAGAGGAGG ATCTGTTTAA CCCATGCCAG GCGATCAAGT GCAAGCATGG GAAATGCAGG 
CTCTCAGGAC TGGGGCAGCC CTACTGTGAA TGCAGCAGTG GATACACCGG GGACAGCTGT 
GATCGAGAAA TCTCTTGTCG AGGGGAACGG ATAAGAGATT ATTACCAAAA GCAGCAGGGC 
TACGCCGCTT GCCAGACGAC CAAGAAGGTG TCTCGGTTGG AATGCAGAGG GGGCTGTGCA 
GGCGGGCAGT GCTGCGGACC TCTGAGGAGC AAGAGAAGGA AATACTCTTT CGAATGCACT 
GATGGGTCCT CGTTTGTGGA CGAGGTGGAG AAGGTGGTAA AGTGTGGCTG TACCCGCTGC 
GCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207849.1
CDS	621..5189
Translation	MHGVGWQTLSLSLGLVLAILNEVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQITTIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPAECTCLDTVVRCSNKALKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLAALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEKDGFWCICADGFEGENCEINVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGYKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYQGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGKNNVAAALRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS

Target ORF information:

RefSeq Version	XM_005207792.4
Organism	Bos taurus(cattle)
Definition	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207792.4

ORF Insert Sequence:

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGG GACTGCTTTG CCGATTTGGC TTGCCCTGAA 
AAGTGCCGCT GCGAAGGGAC CACAGTAGAC TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GATCACATTC CCCAGTACAC TGCAGAGCTG CGCCTCAACA ATAATGAATT TACAGTGTTG 
GAAGCTACCG GGATCTTCAA GAAACTTCCT CAGTTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ACATTGAAGA GGGAGCGTTT GAAGGAGCAT CTGGTGTGAA TGAAATACTT 
CTCACGAGTA ATCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGCTT GGAAAGCCTC 
AAGACTTTGA TGTTGAGAAG TAATCGCATA AGCTGTGTAG GGAATGACAG TTTCATAGGA 
CTCAGCTCTG TGCGTTTGCT TTCTTTATAT GATAATCAGA TTACTACCAT TGCACCAGGA 
GCTTTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTCAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAATGG CTTAGGAAGA AAAGAATTGT AACAGGAAAT 
CCTCGATGTC AGAAACCCTA TTTCCTCAAA GAAATCCCCA TCCAGGATGT GGCCATTCAA 
GACTTCACTT GTGATGATGG AAATGATGAC AATAGCTGTT CCCCACTCTC TCGCTGTCCT 
GCCGAGTGTA CCTGCTTGGA CACAGTGGTT CGATGTAGCA ACAAAGCCTT GAAGGTCTTG 
CCCAAAGGAA TTCCAAGAGA TGTCACTGAA TTGTATCTGG ATGGGAACCA GTTTACCTTG 
GTTCCTAAGG AACTCTCTAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACCC TCTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC TTTAATTCTT 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTGAA GTCTCTTCGG 
TTACTTTCTT TACATGGAAA CGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
GCTGCATTAT CACACCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTAAA GTCGGAATAC AAAGAGCCGG GAATTGCTCG CTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTACTTCTC ACAACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA TCCATGTAAA 
AATGATGGCA CCTGTAACAA TGACCCAGTT GACTTTTATC GCTGCACCTG TCCATATGGT 
TTCAAGGGGC AGGATTGTGA TGTTCCAATT CATGCGTGCA TCAGCAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA AGAAGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAATCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CATGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAC 
ACAGGAGAGT TGTGTGAGGA GAAACTGGAC TTCTGTGCCC AGGACTTGAA CCCCTGCCAG 
CATGACTCCA AGTGCATCCT GACGCCAAAG GGATACAAAT GTGACTGCAC TCCAGGATAC 
ATAGGCGAAC ATTGTGACAT TGACTTCGAT GACTGCCAAG ATAACAAGTG TAAGAACGGA 
GCCCACTGCA CCGATGCAGT GAACGGTTAC ACATGCACCT GTCCTGAAGG CTACAGTGGC 
TTGTTTTGTG AATTTTCTCC ACCTATGGTT CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA ATGCATCATC AGGATCAATG AGCCAATATG CCAGTGTTTG 
CCTGGCTACC AGGGAGAAAA GTGTGAAAAA CTGGTCAGTG TGAATTTTGT AAACAAAGAG 
TCTTATCTTC AGATCCCTTC CGCCAAGGTC CGGCCTCAAA CAAACATCAC TCTTCAGATC 
GCCACAGATG AAGACAGTGG AATCCTCCTG TATAAGGGTG ATAAAGACCA TATTGCTGTA 
GAACTCTACC GAGGACGTGT TCGTGCCAGC TATGACACCG GCTCCCACCC GGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGACGGA AATTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAAAGTC TCTCCCTCTC AGTGGATGGA GGGAGCCCCA AAATCATTAC CAACTTGTCA 
AAACAGTCCA CTCTGAATTT TGACTCCCCA CTCTATGTTG GAGGCATGCC CGGGAAGAAC 
AACGTGGCCG CAGCTCTGCG CCAGGCCCCT GGGCAGAATG GCACCAGCTT CCACGGTTGC 
ATCCGGAACC TTTACATCAA CAGCGAACTT CAGGACTTCC GGAAGGTGCC CATGCAGACC 
GGCATCCTGC CTGGCTGTGA ACCATGCCAC AAGAAGGTGT GTGCCCACGG CACATGCCAG 
CCCAGCAGCC AGGCCGGCTT CACCTGCGAG TGCGAGGAAG GATGGACAGG GCCCCTCTGT 
GATCAGAGGA CCAATGACCC CTGTCTTGGA AATAAATGCG TCCACGGCAC CTGCCTGCCC 
ATCAATGCGT TCTCCTACAG CTGCAAATGC CTAGAGGGCC ATGGGGGCGT CCTCTGTGAT 
GAAGAGGAGG ATCTGTTTAA CCCATGCCAG GCGATCAAGT GCAAGCATGG GAAATGCAGG 
CTCTCAGGAC TGGGGCAGCC CTACTGTGAA TGCAGCAGTG GATACACCGG GGACAGCTGT 
GATCGAGAAA TCTCTTGTCG AGGGGAACGG ATAAGAGATT ATTACCAAAA GCAGCAGGGC 
TACGCCGCTT GCCAGACGAC CAAGAAGGTG TCTCGGTTGG AATGCAGAGG GGGCTGTGCA 
GGCGGGCAGT GCTGCGGACC TCTGAGGAGC AAGAGAAGGA AATACTCTTT CGAATGCACT 
GATGGGTCCT CGTTTGTGGA CGAGGTGGAG AAGGTGGTAA AGTGTGGCTG TACCCGCTGC 
GCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLIT2 ( XM_005207792.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207849.1 Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

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CloneID	OBa20299
Clone ID Related Accession (Same CDS sequence)	XM_005207792.4 , XM_005207792.3
Accession Version	XM_005207792.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	slit homolog 2 protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103970.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207792.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGG GACTGCTTTG CCGATTTGGC TTGCCCTGAA 
AAGTGCCGCT GCGAAGGGAC CACAGTAGAC TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GATCACATTC CCCAGTACAC TGCAGAGCTG CGCCTCAACA ATAATGAATT TACAGTGTTG 
GAAGCTACCG GGATCTTCAA GAAACTTCCT CAGTTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ACATTGAAGA GGGAGCGTTT GAAGGAGCAT CTGGTGTGAA TGAAATACTT 
CTCACGAGTA ATCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGCTT GGAAAGCCTC 
AAGACTTTGA TGTTGAGAAG TAATCGCATA AGCTGTGTAG GGAATGACAG TTTCATAGGA 
CTCAGCTCTG TGCGTTTGCT TTCTTTATAT GATAATCAGA TTACTACCAT TGCACCAGGA 
GCTTTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTCAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAATGG CTTAGGAAGA AAAGAATTGT AACAGGAAAT 
CCTCGATGTC AGAAACCCTA TTTCCTCAAA GAAATCCCCA TCCAGGATGT GGCCATTCAA 
GACTTCACTT GTGATGATGG AAATGATGAC AATAGCTGTT CCCCACTCTC TCGCTGTCCT 
GCCGAGTGTA CCTGCTTGGA CACAGTGGTT CGATGTAGCA ACAAAGCCTT GAAGGTCTTG 
CCCAAAGGAA TTCCAAGAGA TGTCACTGAA TTGTATCTGG ATGGGAACCA GTTTACCTTG 
GTTCCTAAGG AACTCTCTAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACCC TCTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC TTTAATTCTT 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTGAA GTCTCTTCGG 
TTACTTTCTT TACATGGAAA CGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
GCTGCATTAT CACACCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTAAA GTCGGAATAC AAAGAGCCGG GAATTGCTCG CTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTACTTCTC ACAACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA TCCATGTAAA 
AATGATGGCA CCTGTAACAA TGACCCAGTT GACTTTTATC GCTGCACCTG TCCATATGGT 
TTCAAGGGGC AGGATTGTGA TGTTCCAATT CATGCGTGCA TCAGCAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA AGAAGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAATCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CATGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAC 
ACAGGAGAGT TGTGTGAGGA GAAACTGGAC TTCTGTGCCC AGGACTTGAA CCCCTGCCAG 
CATGACTCCA AGTGCATCCT GACGCCAAAG GGATACAAAT GTGACTGCAC TCCAGGATAC 
ATAGGCGAAC ATTGTGACAT TGACTTCGAT GACTGCCAAG ATAACAAGTG TAAGAACGGA 
GCCCACTGCA CCGATGCAGT GAACGGTTAC ACATGCACCT GTCCTGAAGG CTACAGTGGC 
TTGTTTTGTG AATTTTCTCC ACCTATGGTT CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA ATGCATCATC AGGATCAATG AGCCAATATG CCAGTGTTTG 
CCTGGCTACC AGGGAGAAAA GTGTGAAAAA CTGGTCAGTG TGAATTTTGT AAACAAAGAG 
TCTTATCTTC AGATCCCTTC CGCCAAGGTC CGGCCTCAAA CAAACATCAC TCTTCAGATC 
GCCACAGATG AAGACAGTGG AATCCTCCTG TATAAGGGTG ATAAAGACCA TATTGCTGTA 
GAACTCTACC GAGGACGTGT TCGTGCCAGC TATGACACCG GCTCCCACCC GGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGACGGA AATTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAAAGTC TCTCCCTCTC AGTGGATGGA GGGAGCCCCA AAATCATTAC CAACTTGTCA 
AAACAGTCCA CTCTGAATTT TGACTCCCCA CTCTATGTTG GAGGCATGCC CGGGAAGAAC 
AACGTGGCCG CAGCTCTGCG CCAGGCCCCT GGGCAGAATG GCACCAGCTT CCACGGTTGC 
ATCCGGAACC TTTACATCAA CAGCGAACTT CAGGACTTCC GGAAGGTGCC CATGCAGACC 
GGCATCCTGC CTGGCTGTGA ACCATGCCAC AAGAAGGTGT GTGCCCACGG CACATGCCAG 
CCCAGCAGCC AGGCCGGCTT CACCTGCGAG TGCGAGGAAG GATGGACAGG GCCCCTCTGT 
GATCAGAGGA CCAATGACCC CTGTCTTGGA AATAAATGCG TCCACGGCAC CTGCCTGCCC 
ATCAATGCGT TCTCCTACAG CTGCAAATGC CTAGAGGGCC ATGGGGGCGT CCTCTGTGAT 
GAAGAGGAGG ATCTGTTTAA CCCATGCCAG GCGATCAAGT GCAAGCATGG GAAATGCAGG 
CTCTCAGGAC TGGGGCAGCC CTACTGTGAA TGCAGCAGTG GATACACCGG GGACAGCTGT 
GATCGAGAAA TCTCTTGTCG AGGGGAACGG ATAAGAGATT ATTACCAAAA GCAGCAGGGC 
TACGCCGCTT GCCAGACGAC CAAGAAGGTG TCTCGGTTGG AATGCAGAGG GGGCTGTGCA 
GGCGGGCAGT GCTGCGGACC TCTGAGGAGC AAGAGAAGGA AATACTCTTT CGAATGCACT 
GATGGGTCCT CGTTTGTGGA CGAGGTGGAG AAGGTGGTAA AGTGTGGCTG TACCCGCTGC 
GCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207849.1
CDS	616..5184
Translation	MHGVGWQTLSLSLGLVLAILNEVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKITTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTSKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNHISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLTKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLSSVRLLSLYDNQITTIAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPAECTCLDTVVRCSNKALKVLPKGIPRDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLAALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDVNILAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEKDGFWCICADGFEGENCEINVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPKGYKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCTCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYQGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGSPKIITNLSKQSTLNFDSPLYVGGMPGKNNVAAALRQAPGQNGTSFHGCIRNLYINSELQDFRKVPMQTGILPGCEPCHKKVCAHGTCQPSSQAGFTCECEEGWTGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCTRCAS

Target ORF information:

RefSeq Version	XM_005207792.3
Organism	Bos taurus(cattle)
Definition	Bos taurus slit guidance ligand 2 (SLIT2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207792.3

ORF Insert Sequence:

ATGCACGGCG TCGGCTGGCA GACGCTGTCC CTGTCTCTGG GGTTAGTGCT GGCGATCCTG 
AACGAGGTGG CGCCGCAAGC GTGTCCGGCG CAGTGCTCCT GCTCCGGGAG CACAGTGGAC 
TGTCACGGGC TGGCGTTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC CGAGAGATTG 
GATTTGAATG GAAATAACAT CACAAGGATT ACCAAGACAG ATTTTGCTGG TCTTCGACAC 
CTAAGAGTTC TTCAGCTTAT GGAGAATAAG ATTACCACCA TTGAAAGAGG AGCATTCCAG 
GATCTTAAAG AACTGGAGAG ACTGCGTTTA AACAGAAATC ACCTTCAGCT GTTTCCTGAG 
TTGCTGTTTC TTGGGACTTC GAAGCTATAC AGGCTTGACC TCAGTGAAAA CCAGATTCAG 
GCAATTCCAA GGAAAGCTTT TCGTGGGGCA GTTGATATTA AAAATCTGCA ACTGGATTAC 
AACCACATCA GCTGTATTGA AGATGGGGCA TTCAGGGCTC TCCGGGACCT GGAAGTGCTC 
ACTCTCAACA ATAACAACAT TACTAGACTT TCTGTGGCAA GTTTCAACCA TATGCCTAAA 
CTTAGGACTT TTCGACTCCA TTCGAACAAC CTATATTGTG ACTGCCACCT GGCCTGGCTC 
TCGGACTGGC TGCGCCAAAG GCCTCGGGTG GGCCTCTACA CTCAGTGTAT GGGGCCATCT 
CACCTGAGGG GCCACAATGT AGCTGAGGTT CAAAAACGAG AATTTGTCTG CAGCGGTCAC 
CAGTCATTTA TGGCTCCTTC TTGCAGTGTT TTGCACTGCC CAGCTGCTTG TACCTGTAGC 
AACAACATCG TAGATTGCCG TGGGAAAGGT CTCACTGAGA TCCCCACGAA TCTGCCAGAG 
ACCATCACAG AAATACGTTT GGAACAGAAC TCAATCAAGG TCATCCCTCC TGGAGCTTTC 
TCACCATATA AAAAGCTTAG AAGAATCGAC CTGAGCAATA ATCAGATCTC TGAGCTAGCA 
CCAGATGCTT TCCAAGGACT ACGCTCTCTG AATTCACTTG TCCTCTATGG AAATAAAATC 
ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGTTACT ATTACTGAAT 
GCCAACAAGA TAAACTGCCT CCGGGTAGAT GCTTTTCAGG ATCTGCACAA CCTGAACCTT 
CTCTCCTTAT ATGACAACAA GCTTCAGACC ATCGCCAAGG GGACCTTTTC ACCTCTCCGG 
GCCATTCAAA CCATGCATTT GGCCCAGAAC CCCTTTATTT GTGACTGCCA TCTCAAGTGG 
CTGGCGGATT ATCTCCATAC CAACCCAATC GAGACCAGTG GTGCCCGCTG CACCAGTCCC 
CGGCGACTGG CAAACAAAAG AATCGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT 
ACAGAAGATT ATCGATCAAA ATTAAGTGGG GACTGCTTTG CCGATTTGGC TTGCCCTGAA 
AAGTGCCGCT GCGAAGGGAC CACAGTAGAC TGCTCCAATC AAAAACTCAC CAAAATCCCA 
GATCACATTC CCCAGTACAC TGCAGAGCTG CGCCTCAACA ATAATGAATT TACAGTGTTG 
GAAGCTACCG GGATCTTCAA GAAACTTCCT CAGTTACGTA AAATAAACTT TAGCAACAAT 
AAGATCACAG ACATTGAAGA GGGAGCGTTT GAAGGAGCAT CTGGTGTGAA TGAAATACTT 
CTCACGAGTA ATCGTTTGGA AAATGTTCAG CATAAGATGT TCAAGGGCTT GGAAAGCCTC 
AAGACTTTGA TGTTGAGAAG TAATCGCATA AGCTGTGTAG GGAATGACAG TTTCATAGGA 
CTCAGCTCTG TGCGTTTGCT TTCTTTATAT GATAATCAGA TTACTACCAT TGCACCAGGA 
GCTTTTGATA CTCTCCATTC TTTATCTACT CTAAACCTCT TGGCCAATCC TTTCAACTGT 
AACTGCTACC TGGCTTGGTT GGGAGAATGG CTTAGGAAGA AAAGAATTGT AACAGGAAAT 
CCTCGATGTC AGAAACCCTA TTTCCTCAAA GAAATCCCCA TCCAGGATGT GGCCATTCAA 
GACTTCACTT GTGATGATGG AAATGATGAC AATAGCTGTT CCCCACTCTC TCGCTGTCCT 
GCCGAGTGTA CCTGCTTGGA CACAGTGGTT CGATGTAGCA ACAAAGCCTT GAAGGTCTTG 
CCCAAAGGAA TTCCAAGAGA TGTCACTGAA TTGTATCTGG ATGGGAACCA GTTTACCTTG 
GTTCCTAAGG AACTCTCTAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA 
ATAAGCACCC TCTCTAATCA GAGCTTCAGC AACATGACCC AGCTCCTCAC TTTAATTCTT 
AGTTACAACC GTTTGAGATG TATTCCTCCT CGAACCTTCG ATGGACTGAA GTCTCTTCGG 
TTACTTTCTT TACATGGAAA CGACATTTCT GTTGTGCCTG AAGGTGCTTT CAATGATCTT 
GCTGCATTAT CACACCTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG 
TGGTTATCCG ACTGGGTAAA GTCGGAATAC AAAGAGCCGG GAATTGCTCG CTGTGCTGGT 
CCTGGAGAAA TGGCAGATAA ACTACTTCTC ACAACTCCCT CCAAAAAATT TACATGTCAA 
GGTCCTGTGG ATGTCAATAT TCTAGCTAAA TGTAATCCCT GCTTATCAAA TCCATGTAAA 
AATGATGGCA CCTGTAACAA TGACCCAGTT GACTTTTATC GCTGCACCTG TCCATATGGT 
TTCAAGGGGC AGGATTGTGA TGTTCCAATT CATGCGTGCA TCAGCAACCC ATGTAAACAT 
GGAGGAACTT GCCACTTAAA AGAAGGAGAA AAAGATGGAT TCTGGTGTAT TTGTGCTGAT 
GGATTTGAAG GAGAAAATTG TGAAATCAAT GTTGATGACT GTGAAGATAA TGACTGTGAA 
AATAACTCTA CATGTGTCGA TGGAATTAAT AACTACACAT GCCTTTGCCC ACCTGAGTAC 
ACAGGAGAGT TGTGTGAGGA GAAACTGGAC TTCTGTGCCC AGGACTTGAA CCCCTGCCAG 
CATGACTCCA AGTGCATCCT GACGCCAAAG GGATACAAAT GTGACTGCAC TCCAGGATAC 
ATAGGCGAAC ATTGTGACAT TGACTTCGAT GACTGCCAAG ATAACAAGTG TAAGAACGGA 
GCCCACTGCA CCGATGCAGT GAACGGTTAC ACATGCACCT GTCCTGAAGG CTACAGTGGC 
TTGTTTTGTG AATTTTCTCC ACCTATGGTT CTCCCTCGTA CCAGCCCCTG TGATAATTTT 
GATTGTCAGA ATGGAGCTCA ATGCATCATC AGGATCAATG AGCCAATATG CCAGTGTTTG 
CCTGGCTACC AGGGAGAAAA GTGTGAAAAA CTGGTCAGTG TGAATTTTGT AAACAAAGAG 
TCTTATCTTC AGATCCCTTC CGCCAAGGTC CGGCCTCAAA CAAACATCAC TCTTCAGATC 
GCCACAGATG AAGACAGTGG AATCCTCCTG TATAAGGGTG ATAAAGACCA TATTGCTGTA 
GAACTCTACC GAGGACGTGT TCGTGCCAGC TATGACACCG GCTCCCACCC GGCTTCTGCC 
ATTTACAGTG TGGAGACAAT CAATGACGGA AATTTTCACA TTGTGGAACT ACTTGCCCTG 
GATCAAAGTC TCTCCCTCTC AGTGGATGGA GGGAGCCCCA AAATCATTAC CAACTTGTCA 
AAACAGTCCA CTCTGAATTT TGACTCCCCA CTCTATGTTG GAGGCATGCC CGGGAAGAAC 
AACGTGGCCG CAGCTCTGCG CCAGGCCCCT GGGCAGAATG GCACCAGCTT CCACGGTTGC 
ATCCGGAACC TTTACATCAA CAGCGAACTT CAGGACTTCC GGAAGGTGCC CATGCAGACC 
GGCATCCTGC CTGGCTGTGA ACCATGCCAC AAGAAGGTGT GTGCCCACGG CACATGCCAG 
CCCAGCAGCC AGGCCGGCTT CACCTGCGAG TGCGAGGAAG GATGGACAGG GCCCCTCTGT 
GATCAGAGGA CCAATGACCC CTGTCTTGGA AATAAATGCG TCCACGGCAC CTGCCTGCCC 
ATCAATGCGT TCTCCTACAG CTGCAAATGC CTAGAGGGCC ATGGGGGCGT CCTCTGTGAT 
GAAGAGGAGG ATCTGTTTAA CCCATGCCAG GCGATCAAGT GCAAGCATGG GAAATGCAGG 
CTCTCAGGAC TGGGGCAGCC CTACTGTGAA TGCAGCAGTG GATACACCGG GGACAGCTGT 
GATCGAGAAA TCTCTTGTCG AGGGGAACGG ATAAGAGATT ATTACCAAAA GCAGCAGGGC 
TACGCCGCTT GCCAGACGAC CAAGAAGGTG TCTCGGTTGG AATGCAGAGG GGGCTGTGCA 
GGCGGGCAGT GCTGCGGACC TCTGAGGAGC AAGAGAAGGA AATACTCTTT CGAATGCACT 
GATGGGTCCT CGTTTGTGGA CGAGGTGGAG AAGGTGGTAA AGTGTGGCTG TACCCGCTGC 
GCTTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SLIT2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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