sli cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sli
Entrez Gene ID	36746
Full Name	slit
Synonyms	CG33464,CG43758,CG8355,CT21700,CT37068,Dmel\CG43758,Dmel_CG33464,Dmel_CG8355,Sli,Slit,dSlit,fus3,l(2)05248,wdh
General protein information	Preferred Names slit Names CG43758 gene product from transcript CG43758-RD CG43758-PA CG43758-PB CG43758-PC CG43758-PD CG43758-PE CG43758-PF SGP cluster fusion defects 3 lethal (2) 05248 sli-PA sli-PB sli-PC sli-PD sli-PE sli-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 52C9-52D2\|2-77 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_057381.4	NP_476729.1	slit, isoform B
NM_057380.4	NP_476728.1	slit, isoform A
NM_057379.4	NP_476727.1	slit, isoform C
NM_001103864.3	NP_001097334.1	slit, isoform E
NM_001274088.1	NP_001261017.1	slit, isoform F
NM_001103863.3	NP_001097333.1	slit, isoform D

Pathways from BioSystems

Homologies

	SLIT2	XP_002808451.1
Canis lupus familiaris (dog)	SLIT2	XP_005618806.1
Danio rerio (zebrafish)	slit2	NP_571810.1
Xenopus tropicalis (tropical clawed frog)	slit2	XP_004911285.1
Homo sapiens (human)	SLIT2	NP_004778.1
Pan troglodytes (chimpanzee)	SLIT2	XP_003950343.1
Gallus gallus (chicken)	SLIT2	NP_001254004.1
Caenorhabditis elegans (roundworm)	slt-1	NP_510437.2
Bos taurus (cattle)	SLIT2	NP_001178445.2
Mus musculus (house mouse)	Slit2	NP_848919.3
Rattus norvegicus (Norway rat)	Slit2	NP_072154.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002793	XP_312121.5
Drosophila melanogaster (fruit fly)	sli	NP_001261017.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

loss of Atg16 or its binding partner Rab19 impairs Slit production, which normally inhibits enteroendocrine cell generation by activating Robo signaling in stem cells.

repulsive Slit-Robo2-Ena signaling exerts an extrusive force downstream of JNK to eliminate scrib cells from epithelia by disrupting E-cadherin.
Title: Slit-Robo Repulsive Signaling Extrudes Tumorigenic Cells from Epithelia.

the role of Slit and Robo receptors in wiring Drosophila higher-order brain circuits and identified differences in the cellular and molecular mechanisms of Robo/Slit function, was investigated.
Title: Regulation of Drosophila Brain Wiring by Neuropil Interactions via a Slit-Robo-RPTP Signaling Complex.

a Robo2-mediated mechanism, independent of signaling, is essential to limiting Slit distribution and which might be relevant to the regulation of Slit-mediated short-range signaling in additional systems
Title: A non-signaling role of Robo2 in tendons is essential for Slit processing and muscle patterning.

These results suggest that Slit cleaveage, and the formation of Slit-N oligomers on the tendon cell surfaces direct muscle elongation, provide a stop signal for the approaching muscle.
Title: Cleaved Slit directs embryonic muscles.

The following sli gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sli cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa10125	NM_057380.4 Latest version!	Drosophila melanogaster slit (sli), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa10125	NM_001103863.3 Latest version!	Drosophila melanogaster slit (sli), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa10126	NM_001103864.3 Latest version!	Drosophila melanogaster slit (sli), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa10126	NM_057379.4 Latest version!	Drosophila melanogaster slit (sli), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa10127	NM_057381.4 Latest version!	Drosophila melanogaster slit (sli), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa12089	NM_001274088.1 Latest version!	Drosophila melanogaster slit (sli), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sli ( NM_057380.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476728.1 Drosophila melanogaster slit (sli), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa10125
Clone ID Related Accession (Same CDS sequence)	NM_057380.4 , NM_001103863.3
Accession Version	NM_057380.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slit, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057380.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTGG ACATCTCCAA CAATGTCATC 
ACGACCGTGG GTAGACGCGT CTTCAAGGGA GCCCAATCGT TGCGGAGTCT TCAGCTGGAC 
AATAACCAAA TCACCTGCCT GGATGAGCAC GCCTTTAAGG GATTGGTGGA GCTGGAGATA 
CTCACGCTGA ACAACAACAA CCTGACTTCC CTGCCGCACA ACATCTTCGG CGGACTGGGA 
CGTTTGCGGG CACTCCGGCT GTCGGACAAT CCGTTCGCCT GCGACTGCCA TCTGTCCTGG 
CTGTCGCGAT TCCTTCGCAG TGCCACCCGC CTGGCGCCCT ACACCCGCTG CCAGTCGCCA 
TCGCAGCTGA AGGGCCAAAA CGTGGCGGAC CTGCACGACC AGGAGTTCAA ATGCTCGGGT 
CTGACGGAGC ACGCACCGAT GGAATGCGGG GCGGAGAACA GCTGTCCGCA CCCATGTCGC 
TGTGCGGACG GGATCGTCGA TTGCCGTGAG AAGAGTCTGA CCAGCGTGCC CGTCACTTTG 
CCCGACGACA CCACCGAGCT CCGCCTCGAG CAGAATTTCA TTACGGAACT GCCGCCGAAA 
TCGTTCTCCA GCTTTCGACG ACTGCGACGC ATCGACCTGT CCAACAACAA CATATCCCGG 
ATTGCCCACG ATGCACTAAG CGGCCTAAAG CAGTTAACCA CTCTCGTGCT GTACGGCAAT 
AAAATAAAGG ATTTACCCTC GGGCGTGTTC AAAGGACTCG GCTCGCTGCA GCTGCTGCTG 
CTGAACGCCA ACGAGATCTC GTGCATACGC AAGGATGCCT TTCGCGACCT GCACAGTTTG 
AGCCTGCTCT CCCTGTACGA CAACAACATC CAGTCGCTGG CTAATGGCAC ATTCGACGCC 
ATGAAGAGCA TCAAAACGGT ACATCTGGCC AAGAATCCTT TCATCTGCGA CTGCAATCTG 
CGCTGGCTGG CCGACTATTT GCACAAAAAT CCCATAGAGA CGAGTGGAGC CCGCTGCGAG 
TCACCGAAGC GGATGCATCG TCGTCGGATT GAATCGCTGC GCGAGGAGAA ATTCAAATGC 
TCCTGGGATG AATTGCGGAT GAAGCTGTCC GGCGAGTGCC GCATGGACTC CGACTGTCCG 
GCCATGTGCC ACTGCGAGGG CACCACCGTG GATTGCACGG GCCGGGGCCT GAAGGAGATT 
CCGCGCGACA TTCCCCTGCA CACAACTGAG CTTTTGCTCA ACGACAACGA ACTGGGACGC 
ATCAGTTCCG ATGGCCTCTT TGGTCGCCTG CCGCACTTGG TGAAGCTGGA ATTGAAGCGC 
AACCAGCTGA CCGGCATCGA GCCCAACGCC TTCGAGGGAG CATCCCACAT CCAGGAGTTG 
CAGCTGGGCG AGAACAAGAT CAAGGAGATA TCGAACAAGA TGTTCCTGGG ACTGCACCAA 
CTAAAAACGC TCAATCTGTA CGACAATCAA ATCTCATGCG TTATGCCCGG TTCCTTTGAG 
CATCTCAACT CTCTGACGTC GCTGAACCTC GCATCGAATC CATTCAATTG CAATTGTCAT 
TTGGCCTGGT TCGCGGAATG GCTGCGCAAA AAATCACTGA ACGGCGGAGC GGCACGTTGT 
GGAGCCCCGT CGAAGGTACG TGACGTGCAG ATCAAGGACT TGCCCCACTC GGAATTCAAG 
TGTAGCAGCG AGAACAGCGA GGGCTGCCTG GGCGATGGCT ACTGTCCGCC ATCCTGCACC 
TGCACCGGCA CCGTGGTCCG CTGTTCGCGT AACCAGCTGA AGGAGATACC GCGCGGCATT 
CCCGCCGAGA CATCGGAGCT GTATCTGGAG TCCAATGAGA TCGAGCAGAT TCACTACGAA 
CGCATACGCC ATTTGCGCTC CCTTACCCGA CTCGATCTCA GCAACAACCA GATCACCATT 
CTCTCCAACT ACACCTTTGC CAATCTGACC AAGCTGTCCA CGCTCATCAT CTCATACAAC 
AAGCTGCAGT GTCTGCAGCG GCATGCGTTG TCTGGCCTGA ATAACCTGCG CGTGCTTTCG 
CTGCACGGTA ACCGCATCTC GATGCTGCCG GAAGGCTCCT TCGAGGACCT CAAGTCGTTG 
ACCCACATCG CACTAGGCAG CAATCCCTTG TACTGCGATT GCGGTCTGAA GTGGTTCTCC 
GATTGGATAA AGCTGGACTA CGTGGAACCG GGAATTGCAC GTTGCGCCGA ACCGGAACAG 
ATGAAGGATA AGCTGATCCT GTCCACACCC TCGTCGAGCT TCGTTTGCCG CGGCCGCGTG 
CGCAATGATA TTCTGGCCAA GTGCAACGCC TGTTTTGAGC AGCCGTGCCA GAATCAAGCG 
CAGTGCGTGG CCCTTCCGCA GCGAGAGTAC CAGTGCCTCT GCCAGCCGGG CTATCATGGC 
AAACACTGTG AGTTTATGAT CGATGCTTGC TACGGAAATC CGTGCCGCAA CAATGCCACT 
TGCACGGTGC TGGAGGAGGG TCGTTTCAGC TGTCAGTGCG CTCCGGGATA CACAGGTGCC 
CGCTGCGAGA CGAATATCGA CGATTGCCTG GGCGAGATCA AGTGCCAGAA CAATGCCACC 
TGCATCGACG GAGTGGAGTC GTACAAATGT GAGTGCCAGC CGGGATTCAG TGGCGAGTTC 
TGCGACACCA AAATCCAGTT CTGCAGTCCG GAGTTCAATC CCTGCGCGAA TGGAGCCAAG 
TGCATGGACC ACTTTACCCA CTACAGCTGC GATTGTCAGG CAGGTTTCCA TGGCACCAAC 
TGCACGGACA ATATTGACGA CTGCCAGAAC CACATGTGCC AGAACGGTGG AACGTGCGTG 
GACGGCATCA ACGACTACCA ATGCCGCTGT CCAGACGACT ATACGGGCAA GTACTGTGAA 
GGCCACAACA TGATCTCGAT GATGTATCCA CAGACGTCGC CTTGTCAAAA CCACGAGTGC 
AAGCACGGTG TCTGCTTCCA ACCGAACGCT CAGGGCAGCG ACTACCTATG CAGGTGTCAT 
CCGGGTTACA CTGGAAAGTG GTGCGAGTAC CTCACCAGCA TAAGCTTTGT CCACAACAAC 
TCGTTTGTAG AACTGGAGCC ACTGCGAACA CGTCCGGAGG CGAACGTGAC GATAGTCTTC 
AGTAGCGCGG AGCAGAATGG AATTCTCATG TACGACGGCC AGGATGCTCA TCTTGCAGTG 
GAACTGTTTA ATGGGCGTAT TCGGGTTAGC TACGATGTGG GTAATCACCC TGTGTCCACG 
ATGTACAGCT TTGAGATGGT GGCCGATGGA AAGTACCATG CCGTGGAGCT TCTGGCCATC 
AAGAAGAATT TCACGCTGCG CGTGGATCGC GGATTGGCCC GTTCCATCAT CAACGAGGGC 
TCCAACGACT ACCTGAAACT CACGACGCCC ATGTTCCTGG GCGGCCTACC CGTGGATCCT 
GCACAGCAGG CGTACAAGAA CTGGCAAATA CGCAACCTTA CCAGCTTTAA GGGCTGCATG 
AAGGAGGTGT GGATCAATCA TAAGCTGGTC GACTTTGGCA ATGCCCAGCG CCAGCAAAAG 
ATCACACCAG GATGTGCCCT GCTCGAAGGA GAGCAGCAAG AGGAGGAAGA CGACGAGCAG 
GATTTCATGG ACGAGACACC GCACATCAAA GAGGAGCCGG TGGATCCTTG CCTGGAGAAC 
AAATGCCGTC GGGGCAGTCG CTGTGTGCCG AATTCCAATG CCAGGGACGG CTACCAGTGC 
AAGTGCAAGC ACGGCCAGCG CGGCCGCTAC TGCGATCAAG GTGAGGGCAG CACTGAGCCC 
CCAACAGTCA CCGCGGCGTC CACCTGTCGC AAGGAGCAGG TGCGCGAGTA CTACACGGAG 
AACGACTGTC GCTCGAGGCA GCCGTTGAAG TACGCCAAGT GCGTGGGCGG CTGCGGCAAC 
CAGTGCTGCG CGGCCAAAAT TGTGAGACGG CGCAAGGTGC GCATGGTGTG CAGCAACAAC 
CGCAAGTACA TCAAGAACTT GGACATCGTG CGCAAGTGCG GATGCACCAA GAAATGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476728.1
CDS	1158..5600
Translation	MAAPSRTTLMPPPFRLQLRLLILPILLLLRHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY

Target ORF information:

RefSeq Version	NM_057380.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slit (sli), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057380.4

ORF Insert Sequence:

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTGG ACATCTCCAA CAATGTCATC 
ACGACCGTGG GTAGACGCGT CTTCAAGGGA GCCCAATCGT TGCGGAGTCT TCAGCTGGAC 
AATAACCAAA TCACCTGCCT GGATGAGCAC GCCTTTAAGG GATTGGTGGA GCTGGAGATA 
CTCACGCTGA ACAACAACAA CCTGACTTCC CTGCCGCACA ACATCTTCGG CGGACTGGGA 
CGTTTGCGGG CACTCCGGCT GTCGGACAAT CCGTTCGCCT GCGACTGCCA TCTGTCCTGG 
CTGTCGCGAT TCCTTCGCAG TGCCACCCGC CTGGCGCCCT ACACCCGCTG CCAGTCGCCA 
TCGCAGCTGA AGGGCCAAAA CGTGGCGGAC CTGCACGACC AGGAGTTCAA ATGCTCGGGT 
CTGACGGAGC ACGCACCGAT GGAATGCGGG GCGGAGAACA GCTGTCCGCA CCCATGTCGC 
TGTGCGGACG GGATCGTCGA TTGCCGTGAG AAGAGTCTGA CCAGCGTGCC CGTCACTTTG 
CCCGACGACA CCACCGAGCT CCGCCTCGAG CAGAATTTCA TTACGGAACT GCCGCCGAAA 
TCGTTCTCCA GCTTTCGACG ACTGCGACGC ATCGACCTGT CCAACAACAA CATATCCCGG 
ATTGCCCACG ATGCACTAAG CGGCCTAAAG CAGTTAACCA CTCTCGTGCT GTACGGCAAT 
AAAATAAAGG ATTTACCCTC GGGCGTGTTC AAAGGACTCG GCTCGCTGCA GCTGCTGCTG 
CTGAACGCCA ACGAGATCTC GTGCATACGC AAGGATGCCT TTCGCGACCT GCACAGTTTG 
AGCCTGCTCT CCCTGTACGA CAACAACATC CAGTCGCTGG CTAATGGCAC ATTCGACGCC 
ATGAAGAGCA TCAAAACGGT ACATCTGGCC AAGAATCCTT TCATCTGCGA CTGCAATCTG 
CGCTGGCTGG CCGACTATTT GCACAAAAAT CCCATAGAGA CGAGTGGAGC CCGCTGCGAG 
TCACCGAAGC GGATGCATCG TCGTCGGATT GAATCGCTGC GCGAGGAGAA ATTCAAATGC 
TCCTGGGATG AATTGCGGAT GAAGCTGTCC GGCGAGTGCC GCATGGACTC CGACTGTCCG 
GCCATGTGCC ACTGCGAGGG CACCACCGTG GATTGCACGG GCCGGGGCCT GAAGGAGATT 
CCGCGCGACA TTCCCCTGCA CACAACTGAG CTTTTGCTCA ACGACAACGA ACTGGGACGC 
ATCAGTTCCG ATGGCCTCTT TGGTCGCCTG CCGCACTTGG TGAAGCTGGA ATTGAAGCGC 
AACCAGCTGA CCGGCATCGA GCCCAACGCC TTCGAGGGAG CATCCCACAT CCAGGAGTTG 
CAGCTGGGCG AGAACAAGAT CAAGGAGATA TCGAACAAGA TGTTCCTGGG ACTGCACCAA 
CTAAAAACGC TCAATCTGTA CGACAATCAA ATCTCATGCG TTATGCCCGG TTCCTTTGAG 
CATCTCAACT CTCTGACGTC GCTGAACCTC GCATCGAATC CATTCAATTG CAATTGTCAT 
TTGGCCTGGT TCGCGGAATG GCTGCGCAAA AAATCACTGA ACGGCGGAGC GGCACGTTGT 
GGAGCCCCGT CGAAGGTACG TGACGTGCAG ATCAAGGACT TGCCCCACTC GGAATTCAAG 
TGTAGCAGCG AGAACAGCGA GGGCTGCCTG GGCGATGGCT ACTGTCCGCC ATCCTGCACC 
TGCACCGGCA CCGTGGTCCG CTGTTCGCGT AACCAGCTGA AGGAGATACC GCGCGGCATT 
CCCGCCGAGA CATCGGAGCT GTATCTGGAG TCCAATGAGA TCGAGCAGAT TCACTACGAA 
CGCATACGCC ATTTGCGCTC CCTTACCCGA CTCGATCTCA GCAACAACCA GATCACCATT 
CTCTCCAACT ACACCTTTGC CAATCTGACC AAGCTGTCCA CGCTCATCAT CTCATACAAC 
AAGCTGCAGT GTCTGCAGCG GCATGCGTTG TCTGGCCTGA ATAACCTGCG CGTGCTTTCG 
CTGCACGGTA ACCGCATCTC GATGCTGCCG GAAGGCTCCT TCGAGGACCT CAAGTCGTTG 
ACCCACATCG CACTAGGCAG CAATCCCTTG TACTGCGATT GCGGTCTGAA GTGGTTCTCC 
GATTGGATAA AGCTGGACTA CGTGGAACCG GGAATTGCAC GTTGCGCCGA ACCGGAACAG 
ATGAAGGATA AGCTGATCCT GTCCACACCC TCGTCGAGCT TCGTTTGCCG CGGCCGCGTG 
CGCAATGATA TTCTGGCCAA GTGCAACGCC TGTTTTGAGC AGCCGTGCCA GAATCAAGCG 
CAGTGCGTGG CCCTTCCGCA GCGAGAGTAC CAGTGCCTCT GCCAGCCGGG CTATCATGGC 
AAACACTGTG AGTTTATGAT CGATGCTTGC TACGGAAATC CGTGCCGCAA CAATGCCACT 
TGCACGGTGC TGGAGGAGGG TCGTTTCAGC TGTCAGTGCG CTCCGGGATA CACAGGTGCC 
CGCTGCGAGA CGAATATCGA CGATTGCCTG GGCGAGATCA AGTGCCAGAA CAATGCCACC 
TGCATCGACG GAGTGGAGTC GTACAAATGT GAGTGCCAGC CGGGATTCAG TGGCGAGTTC 
TGCGACACCA AAATCCAGTT CTGCAGTCCG GAGTTCAATC CCTGCGCGAA TGGAGCCAAG 
TGCATGGACC ACTTTACCCA CTACAGCTGC GATTGTCAGG CAGGTTTCCA TGGCACCAAC 
TGCACGGACA ATATTGACGA CTGCCAGAAC CACATGTGCC AGAACGGTGG AACGTGCGTG 
GACGGCATCA ACGACTACCA ATGCCGCTGT CCAGACGACT ATACGGGCAA GTACTGTGAA 
GGCCACAACA TGATCTCGAT GATGTATCCA CAGACGTCGC CTTGTCAAAA CCACGAGTGC 
AAGCACGGTG TCTGCTTCCA ACCGAACGCT CAGGGCAGCG ACTACCTATG CAGGTGTCAT 
CCGGGTTACA CTGGAAAGTG GTGCGAGTAC CTCACCAGCA TAAGCTTTGT CCACAACAAC 
TCGTTTGTAG AACTGGAGCC ACTGCGAACA CGTCCGGAGG CGAACGTGAC GATAGTCTTC 
AGTAGCGCGG AGCAGAATGG AATTCTCATG TACGACGGCC AGGATGCTCA TCTTGCAGTG 
GAACTGTTTA ATGGGCGTAT TCGGGTTAGC TACGATGTGG GTAATCACCC TGTGTCCACG 
ATGTACAGCT TTGAGATGGT GGCCGATGGA AAGTACCATG CCGTGGAGCT TCTGGCCATC 
AAGAAGAATT TCACGCTGCG CGTGGATCGC GGATTGGCCC GTTCCATCAT CAACGAGGGC 
TCCAACGACT ACCTGAAACT CACGACGCCC ATGTTCCTGG GCGGCCTACC CGTGGATCCT 
GCACAGCAGG CGTACAAGAA CTGGCAAATA CGCAACCTTA CCAGCTTTAA GGGCTGCATG 
AAGGAGGTGT GGATCAATCA TAAGCTGGTC GACTTTGGCA ATGCCCAGCG CCAGCAAAAG 
ATCACACCAG GATGTGCCCT GCTCGAAGGA GAGCAGCAAG AGGAGGAAGA CGACGAGCAG 
GATTTCATGG ACGAGACACC GCACATCAAA GAGGAGCCGG TGGATCCTTG CCTGGAGAAC 
AAATGCCGTC GGGGCAGTCG CTGTGTGCCG AATTCCAATG CCAGGGACGG CTACCAGTGC 
AAGTGCAAGC ACGGCCAGCG CGGCCGCTAC TGCGATCAAG GTGAGGGCAG CACTGAGCCC 
CCAACAGTCA CCGCGGCGTC CACCTGTCGC AAGGAGCAGG TGCGCGAGTA CTACACGGAG 
AACGACTGTC GCTCGAGGCA GCCGTTGAAG TACGCCAAGT GCGTGGGCGG CTGCGGCAAC 
CAGTGCTGCG CGGCCAAAAT TGTGAGACGG CGCAAGGTGC GCATGGTGTG CAGCAACAAC 
CGCAAGTACA TCAAGAACTT GGACATCGTG CGCAAGTGCG GATGCACCAA GAAATGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sli ( NM_001103863.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097333.1 Drosophila melanogaster slit (sli), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa10125
Clone ID Related Accession (Same CDS sequence)	NM_057380.4 , NM_001103863.3
Accession Version	NM_001103863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slit, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103863.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTGG ACATCTCCAA CAATGTCATC 
ACGACCGTGG GTAGACGCGT CTTCAAGGGA GCCCAATCGT TGCGGAGTCT TCAGCTGGAC 
AATAACCAAA TCACCTGCCT GGATGAGCAC GCCTTTAAGG GATTGGTGGA GCTGGAGATA 
CTCACGCTGA ACAACAACAA CCTGACTTCC CTGCCGCACA ACATCTTCGG CGGACTGGGA 
CGTTTGCGGG CACTCCGGCT GTCGGACAAT CCGTTCGCCT GCGACTGCCA TCTGTCCTGG 
CTGTCGCGAT TCCTTCGCAG TGCCACCCGC CTGGCGCCCT ACACCCGCTG CCAGTCGCCA 
TCGCAGCTGA AGGGCCAAAA CGTGGCGGAC CTGCACGACC AGGAGTTCAA ATGCTCGGGT 
CTGACGGAGC ACGCACCGAT GGAATGCGGG GCGGAGAACA GCTGTCCGCA CCCATGTCGC 
TGTGCGGACG GGATCGTCGA TTGCCGTGAG AAGAGTCTGA CCAGCGTGCC CGTCACTTTG 
CCCGACGACA CCACCGAGCT CCGCCTCGAG CAGAATTTCA TTACGGAACT GCCGCCGAAA 
TCGTTCTCCA GCTTTCGACG ACTGCGACGC ATCGACCTGT CCAACAACAA CATATCCCGG 
ATTGCCCACG ATGCACTAAG CGGCCTAAAG CAGTTAACCA CTCTCGTGCT GTACGGCAAT 
AAAATAAAGG ATTTACCCTC GGGCGTGTTC AAAGGACTCG GCTCGCTGCA GCTGCTGCTG 
CTGAACGCCA ACGAGATCTC GTGCATACGC AAGGATGCCT TTCGCGACCT GCACAGTTTG 
AGCCTGCTCT CCCTGTACGA CAACAACATC CAGTCGCTGG CTAATGGCAC ATTCGACGCC 
ATGAAGAGCA TCAAAACGGT ACATCTGGCC AAGAATCCTT TCATCTGCGA CTGCAATCTG 
CGCTGGCTGG CCGACTATTT GCACAAAAAT CCCATAGAGA CGAGTGGAGC CCGCTGCGAG 
TCACCGAAGC GGATGCATCG TCGTCGGATT GAATCGCTGC GCGAGGAGAA ATTCAAATGC 
TCCTGGGATG AATTGCGGAT GAAGCTGTCC GGCGAGTGCC GCATGGACTC CGACTGTCCG 
GCCATGTGCC ACTGCGAGGG CACCACCGTG GATTGCACGG GCCGGGGCCT GAAGGAGATT 
CCGCGCGACA TTCCCCTGCA CACAACTGAG CTTTTGCTCA ACGACAACGA ACTGGGACGC 
ATCAGTTCCG ATGGCCTCTT TGGTCGCCTG CCGCACTTGG TGAAGCTGGA ATTGAAGCGC 
AACCAGCTGA CCGGCATCGA GCCCAACGCC TTCGAGGGAG CATCCCACAT CCAGGAGTTG 
CAGCTGGGCG AGAACAAGAT CAAGGAGATA TCGAACAAGA TGTTCCTGGG ACTGCACCAA 
CTAAAAACGC TCAATCTGTA CGACAATCAA ATCTCATGCG TTATGCCCGG TTCCTTTGAG 
CATCTCAACT CTCTGACGTC GCTGAACCTC GCATCGAATC CATTCAATTG CAATTGTCAT 
TTGGCCTGGT TCGCGGAATG GCTGCGCAAA AAATCACTGA ACGGCGGAGC GGCACGTTGT 
GGAGCCCCGT CGAAGGTACG TGACGTGCAG ATCAAGGACT TGCCCCACTC GGAATTCAAG 
TGTAGCAGCG AGAACAGCGA GGGCTGCCTG GGCGATGGCT ACTGTCCGCC ATCCTGCACC 
TGCACCGGCA CCGTGGTCCG CTGTTCGCGT AACCAGCTGA AGGAGATACC GCGCGGCATT 
CCCGCCGAGA CATCGGAGCT GTATCTGGAG TCCAATGAGA TCGAGCAGAT TCACTACGAA 
CGCATACGCC ATTTGCGCTC CCTTACCCGA CTCGATCTCA GCAACAACCA GATCACCATT 
CTCTCCAACT ACACCTTTGC CAATCTGACC AAGCTGTCCA CGCTCATCAT CTCATACAAC 
AAGCTGCAGT GTCTGCAGCG GCATGCGTTG TCTGGCCTGA ATAACCTGCG CGTGCTTTCG 
CTGCACGGTA ACCGCATCTC GATGCTGCCG GAAGGCTCCT TCGAGGACCT CAAGTCGTTG 
ACCCACATCG CACTAGGCAG CAATCCCTTG TACTGCGATT GCGGTCTGAA GTGGTTCTCC 
GATTGGATAA AGCTGGACTA CGTGGAACCG GGAATTGCAC GTTGCGCCGA ACCGGAACAG 
ATGAAGGATA AGCTGATCCT GTCCACACCC TCGTCGAGCT TCGTTTGCCG CGGCCGCGTG 
CGCAATGATA TTCTGGCCAA GTGCAACGCC TGTTTTGAGC AGCCGTGCCA GAATCAAGCG 
CAGTGCGTGG CCCTTCCGCA GCGAGAGTAC CAGTGCCTCT GCCAGCCGGG CTATCATGGC 
AAACACTGTG AGTTTATGAT CGATGCTTGC TACGGAAATC CGTGCCGCAA CAATGCCACT 
TGCACGGTGC TGGAGGAGGG TCGTTTCAGC TGTCAGTGCG CTCCGGGATA CACAGGTGCC 
CGCTGCGAGA CGAATATCGA CGATTGCCTG GGCGAGATCA AGTGCCAGAA CAATGCCACC 
TGCATCGACG GAGTGGAGTC GTACAAATGT GAGTGCCAGC CGGGATTCAG TGGCGAGTTC 
TGCGACACCA AAATCCAGTT CTGCAGTCCG GAGTTCAATC CCTGCGCGAA TGGAGCCAAG 
TGCATGGACC ACTTTACCCA CTACAGCTGC GATTGTCAGG CAGGTTTCCA TGGCACCAAC 
TGCACGGACA ATATTGACGA CTGCCAGAAC CACATGTGCC AGAACGGTGG AACGTGCGTG 
GACGGCATCA ACGACTACCA ATGCCGCTGT CCAGACGACT ATACGGGCAA GTACTGTGAA 
GGCCACAACA TGATCTCGAT GATGTATCCA CAGACGTCGC CTTGTCAAAA CCACGAGTGC 
AAGCACGGTG TCTGCTTCCA ACCGAACGCT CAGGGCAGCG ACTACCTATG CAGGTGTCAT 
CCGGGTTACA CTGGAAAGTG GTGCGAGTAC CTCACCAGCA TAAGCTTTGT CCACAACAAC 
TCGTTTGTAG AACTGGAGCC ACTGCGAACA CGTCCGGAGG CGAACGTGAC GATAGTCTTC 
AGTAGCGCGG AGCAGAATGG AATTCTCATG TACGACGGCC AGGATGCTCA TCTTGCAGTG 
GAACTGTTTA ATGGGCGTAT TCGGGTTAGC TACGATGTGG GTAATCACCC TGTGTCCACG 
ATGTACAGCT TTGAGATGGT GGCCGATGGA AAGTACCATG CCGTGGAGCT TCTGGCCATC 
AAGAAGAATT TCACGCTGCG CGTGGATCGC GGATTGGCCC GTTCCATCAT CAACGAGGGC 
TCCAACGACT ACCTGAAACT CACGACGCCC ATGTTCCTGG GCGGCCTACC CGTGGATCCT 
GCACAGCAGG CGTACAAGAA CTGGCAAATA CGCAACCTTA CCAGCTTTAA GGGCTGCATG 
AAGGAGGTGT GGATCAATCA TAAGCTGGTC GACTTTGGCA ATGCCCAGCG CCAGCAAAAG 
ATCACACCAG GATGTGCCCT GCTCGAAGGA GAGCAGCAAG AGGAGGAAGA CGACGAGCAG 
GATTTCATGG ACGAGACACC GCACATCAAA GAGGAGCCGG TGGATCCTTG CCTGGAGAAC 
AAATGCCGTC GGGGCAGTCG CTGTGTGCCG AATTCCAATG CCAGGGACGG CTACCAGTGC 
AAGTGCAAGC ACGGCCAGCG CGGCCGCTAC TGCGATCAAG GTGAGGGCAG CACTGAGCCC 
CCAACAGTCA CCGCGGCGTC CACCTGTCGC AAGGAGCAGG TGCGCGAGTA CTACACGGAG 
AACGACTGTC GCTCGAGGCA GCCGTTGAAG TACGCCAAGT GCGTGGGCGG CTGCGGCAAC 
CAGTGCTGCG CGGCCAAAAT TGTGAGACGG CGCAAGGTGC GCATGGTGTG CAGCAACAAC 
CGCAAGTACA TCAAGAACTT GGACATCGTG CGCAAGTGCG GATGCACCAA GAAATGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097333.1
CDS	1158..5600
Translation	MAAPSRTTLMPPPFRLQLRLLILPILLLLRHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY

Target ORF information:

RefSeq Version	NM_001103863.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slit (sli), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103863.3

ORF Insert Sequence:

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTGG ACATCTCCAA CAATGTCATC 
ACGACCGTGG GTAGACGCGT CTTCAAGGGA GCCCAATCGT TGCGGAGTCT TCAGCTGGAC 
AATAACCAAA TCACCTGCCT GGATGAGCAC GCCTTTAAGG GATTGGTGGA GCTGGAGATA 
CTCACGCTGA ACAACAACAA CCTGACTTCC CTGCCGCACA ACATCTTCGG CGGACTGGGA 
CGTTTGCGGG CACTCCGGCT GTCGGACAAT CCGTTCGCCT GCGACTGCCA TCTGTCCTGG 
CTGTCGCGAT TCCTTCGCAG TGCCACCCGC CTGGCGCCCT ACACCCGCTG CCAGTCGCCA 
TCGCAGCTGA AGGGCCAAAA CGTGGCGGAC CTGCACGACC AGGAGTTCAA ATGCTCGGGT 
CTGACGGAGC ACGCACCGAT GGAATGCGGG GCGGAGAACA GCTGTCCGCA CCCATGTCGC 
TGTGCGGACG GGATCGTCGA TTGCCGTGAG AAGAGTCTGA CCAGCGTGCC CGTCACTTTG 
CCCGACGACA CCACCGAGCT CCGCCTCGAG CAGAATTTCA TTACGGAACT GCCGCCGAAA 
TCGTTCTCCA GCTTTCGACG ACTGCGACGC ATCGACCTGT CCAACAACAA CATATCCCGG 
ATTGCCCACG ATGCACTAAG CGGCCTAAAG CAGTTAACCA CTCTCGTGCT GTACGGCAAT 
AAAATAAAGG ATTTACCCTC GGGCGTGTTC AAAGGACTCG GCTCGCTGCA GCTGCTGCTG 
CTGAACGCCA ACGAGATCTC GTGCATACGC AAGGATGCCT TTCGCGACCT GCACAGTTTG 
AGCCTGCTCT CCCTGTACGA CAACAACATC CAGTCGCTGG CTAATGGCAC ATTCGACGCC 
ATGAAGAGCA TCAAAACGGT ACATCTGGCC AAGAATCCTT TCATCTGCGA CTGCAATCTG 
CGCTGGCTGG CCGACTATTT GCACAAAAAT CCCATAGAGA CGAGTGGAGC CCGCTGCGAG 
TCACCGAAGC GGATGCATCG TCGTCGGATT GAATCGCTGC GCGAGGAGAA ATTCAAATGC 
TCCTGGGATG AATTGCGGAT GAAGCTGTCC GGCGAGTGCC GCATGGACTC CGACTGTCCG 
GCCATGTGCC ACTGCGAGGG CACCACCGTG GATTGCACGG GCCGGGGCCT GAAGGAGATT 
CCGCGCGACA TTCCCCTGCA CACAACTGAG CTTTTGCTCA ACGACAACGA ACTGGGACGC 
ATCAGTTCCG ATGGCCTCTT TGGTCGCCTG CCGCACTTGG TGAAGCTGGA ATTGAAGCGC 
AACCAGCTGA CCGGCATCGA GCCCAACGCC TTCGAGGGAG CATCCCACAT CCAGGAGTTG 
CAGCTGGGCG AGAACAAGAT CAAGGAGATA TCGAACAAGA TGTTCCTGGG ACTGCACCAA 
CTAAAAACGC TCAATCTGTA CGACAATCAA ATCTCATGCG TTATGCCCGG TTCCTTTGAG 
CATCTCAACT CTCTGACGTC GCTGAACCTC GCATCGAATC CATTCAATTG CAATTGTCAT 
TTGGCCTGGT TCGCGGAATG GCTGCGCAAA AAATCACTGA ACGGCGGAGC GGCACGTTGT 
GGAGCCCCGT CGAAGGTACG TGACGTGCAG ATCAAGGACT TGCCCCACTC GGAATTCAAG 
TGTAGCAGCG AGAACAGCGA GGGCTGCCTG GGCGATGGCT ACTGTCCGCC ATCCTGCACC 
TGCACCGGCA CCGTGGTCCG CTGTTCGCGT AACCAGCTGA AGGAGATACC GCGCGGCATT 
CCCGCCGAGA CATCGGAGCT GTATCTGGAG TCCAATGAGA TCGAGCAGAT TCACTACGAA 
CGCATACGCC ATTTGCGCTC CCTTACCCGA CTCGATCTCA GCAACAACCA GATCACCATT 
CTCTCCAACT ACACCTTTGC CAATCTGACC AAGCTGTCCA CGCTCATCAT CTCATACAAC 
AAGCTGCAGT GTCTGCAGCG GCATGCGTTG TCTGGCCTGA ATAACCTGCG CGTGCTTTCG 
CTGCACGGTA ACCGCATCTC GATGCTGCCG GAAGGCTCCT TCGAGGACCT CAAGTCGTTG 
ACCCACATCG CACTAGGCAG CAATCCCTTG TACTGCGATT GCGGTCTGAA GTGGTTCTCC 
GATTGGATAA AGCTGGACTA CGTGGAACCG GGAATTGCAC GTTGCGCCGA ACCGGAACAG 
ATGAAGGATA AGCTGATCCT GTCCACACCC TCGTCGAGCT TCGTTTGCCG CGGCCGCGTG 
CGCAATGATA TTCTGGCCAA GTGCAACGCC TGTTTTGAGC AGCCGTGCCA GAATCAAGCG 
CAGTGCGTGG CCCTTCCGCA GCGAGAGTAC CAGTGCCTCT GCCAGCCGGG CTATCATGGC 
AAACACTGTG AGTTTATGAT CGATGCTTGC TACGGAAATC CGTGCCGCAA CAATGCCACT 
TGCACGGTGC TGGAGGAGGG TCGTTTCAGC TGTCAGTGCG CTCCGGGATA CACAGGTGCC 
CGCTGCGAGA CGAATATCGA CGATTGCCTG GGCGAGATCA AGTGCCAGAA CAATGCCACC 
TGCATCGACG GAGTGGAGTC GTACAAATGT GAGTGCCAGC CGGGATTCAG TGGCGAGTTC 
TGCGACACCA AAATCCAGTT CTGCAGTCCG GAGTTCAATC CCTGCGCGAA TGGAGCCAAG 
TGCATGGACC ACTTTACCCA CTACAGCTGC GATTGTCAGG CAGGTTTCCA TGGCACCAAC 
TGCACGGACA ATATTGACGA CTGCCAGAAC CACATGTGCC AGAACGGTGG AACGTGCGTG 
GACGGCATCA ACGACTACCA ATGCCGCTGT CCAGACGACT ATACGGGCAA GTACTGTGAA 
GGCCACAACA TGATCTCGAT GATGTATCCA CAGACGTCGC CTTGTCAAAA CCACGAGTGC 
AAGCACGGTG TCTGCTTCCA ACCGAACGCT CAGGGCAGCG ACTACCTATG CAGGTGTCAT 
CCGGGTTACA CTGGAAAGTG GTGCGAGTAC CTCACCAGCA TAAGCTTTGT CCACAACAAC 
TCGTTTGTAG AACTGGAGCC ACTGCGAACA CGTCCGGAGG CGAACGTGAC GATAGTCTTC 
AGTAGCGCGG AGCAGAATGG AATTCTCATG TACGACGGCC AGGATGCTCA TCTTGCAGTG 
GAACTGTTTA ATGGGCGTAT TCGGGTTAGC TACGATGTGG GTAATCACCC TGTGTCCACG 
ATGTACAGCT TTGAGATGGT GGCCGATGGA AAGTACCATG CCGTGGAGCT TCTGGCCATC 
AAGAAGAATT TCACGCTGCG CGTGGATCGC GGATTGGCCC GTTCCATCAT CAACGAGGGC 
TCCAACGACT ACCTGAAACT CACGACGCCC ATGTTCCTGG GCGGCCTACC CGTGGATCCT 
GCACAGCAGG CGTACAAGAA CTGGCAAATA CGCAACCTTA CCAGCTTTAA GGGCTGCATG 
AAGGAGGTGT GGATCAATCA TAAGCTGGTC GACTTTGGCA ATGCCCAGCG CCAGCAAAAG 
ATCACACCAG GATGTGCCCT GCTCGAAGGA GAGCAGCAAG AGGAGGAAGA CGACGAGCAG 
GATTTCATGG ACGAGACACC GCACATCAAA GAGGAGCCGG TGGATCCTTG CCTGGAGAAC 
AAATGCCGTC GGGGCAGTCG CTGTGTGCCG AATTCCAATG CCAGGGACGG CTACCAGTGC 
AAGTGCAAGC ACGGCCAGCG CGGCCGCTAC TGCGATCAAG GTGAGGGCAG CACTGAGCCC 
CCAACAGTCA CCGCGGCGTC CACCTGTCGC AAGGAGCAGG TGCGCGAGTA CTACACGGAG 
AACGACTGTC GCTCGAGGCA GCCGTTGAAG TACGCCAAGT GCGTGGGCGG CTGCGGCAAC 
CAGTGCTGCG CGGCCAAAAT TGTGAGACGG CGCAAGGTGC GCATGGTGTG CAGCAACAAC 
CGCAAGTACA TCAAGAACTT GGACATCGTG CGCAAGTGCG GATGCACCAA GAAATGCTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sli ( NM_057379.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476727.1 Drosophila melanogaster slit (sli), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa10126
Clone ID Related Accession (Same CDS sequence)	NM_057379.4 , NM_001103864.3
Accession Version	NM_057379.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slit, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057379.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT TGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACGACCGT GGGTAGACGC GTCTTCAAGG GAGCCCAATC GTTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGC CTGGATGAGC ACGCCTTTAA GGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAACAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTTTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCGCG ATTCCTTCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGTGCGGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACTT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAATTT CATTACGGAA 
CTGCCGCCGA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATATCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGCTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTTCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCCATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACAACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGTCGCC TGCCGCACTT GGTGAAGCTG 
GAATTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG AGCATCCCAC 
ATCCAGGAGT TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TATCGAACAA GATGTTCCTG 
GGACTGCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCATG CGTTATGCCC 
GGTTCCTTTG AGCATCTCAA CTCTCTGACG TCGCTGAACC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGCGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CTTGCCCCAC 
TCGGAATTCA AGTGTAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTC CGCTGTTCGC GTAACCAGCT GAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATACG CCATTTGCGC TCCCTTACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTT GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CGCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAC 
CTCAAGTCGT TGACCCACAT CGCACTAGGC AGCAATCCCT TGTACTGCGA TTGCGGTCTG 
AAGTGGTTCT CCGATTGGAT AAAGCTGGAC TACGTGGAAC CGGGAATTGC ACGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACAC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGCAACG CCTGTTTTGA GCAGCCGTGC 
CAGAATCAAG CGCAGTGCGT GGCCCTTCCG CAGCGAGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG TGAGTTTATG ATCGATGCTT GCTACGGAAA TCCGTGCCGC 
AACAATGCCA CTTGCACGGT GCTGGAGGAG GGTCGTTTCA GCTGTCAGTG CGCTCCGGGA 
TACACAGGTG CCCGCTGCGA GACGAATATC GACGATTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CCTGCATCGA CGGAGTGGAG TCGTACAAAT GTGAGTGCCA GCCGGGATTC 
AGTGGCGAGT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CGGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATGGA CCACTTTACC CACTACAGCT GCGATTGTCA GGCAGGTTTC 
CATGGCACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGT 
GGAACGTGCG TGGACGGCAT CAACGACTAC CAATGCCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGCCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGTCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGTTA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATAAGCTTT 
GTCCACAACA ACTCGTTTGT AGAACTGGAG CCACTGCGAA CACGTCCGGA GGCGAACGTG 
ACGATAGTCT TCAGTAGCGC GGAGCAGAAT GGAATTCTCA TGTACGACGG CCAGGATGCT 
CATCTTGCAG TGGAACTGTT TAATGGGCGT ATTCGGGTTA GCTACGATGT GGGTAATCAC 
CCTGTGTCCA CGATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCCGTGGAG 
CTTCTGGCCA TCAAGAAGAA TTTCACGCTG CGCGTGGATC GCGGATTGGC CCGTTCCATC 
ATCAACGAGG GCTCCAACGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGCCTA 
CCCGTGGATC CTGCACAGCA GGCGTACAAG AACTGGCAAA TACGCAACCT TACCAGCTTT 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAT CATAAGCTGG TCGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC AGGATGTGCC CTGCTCGAAG GAGAGCAGCA AGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACA CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAATGCCG TCGGGGCAGT CGCTGTGTGC CGAATTCCAA TGCCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTACACGG AGAACGACTG TCGCTCGAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAA ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476727.1
CDS	1158..5672
Translation	MAAPSRTTLMPPPFRLQLRLLILPILLLLRHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY

Target ORF information:

RefSeq Version	NM_057379.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slit (sli), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057379.4

ORF Insert Sequence:

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT TGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACGACCGT GGGTAGACGC GTCTTCAAGG GAGCCCAATC GTTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGC CTGGATGAGC ACGCCTTTAA GGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAACAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTTTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCGCG ATTCCTTCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGTGCGGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACTT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAATTT CATTACGGAA 
CTGCCGCCGA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATATCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGCTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTTCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCCATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACAACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGTCGCC TGCCGCACTT GGTGAAGCTG 
GAATTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG AGCATCCCAC 
ATCCAGGAGT TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TATCGAACAA GATGTTCCTG 
GGACTGCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCATG CGTTATGCCC 
GGTTCCTTTG AGCATCTCAA CTCTCTGACG TCGCTGAACC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGCGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CTTGCCCCAC 
TCGGAATTCA AGTGTAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTC CGCTGTTCGC GTAACCAGCT GAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATACG CCATTTGCGC TCCCTTACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTT GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CGCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAC 
CTCAAGTCGT TGACCCACAT CGCACTAGGC AGCAATCCCT TGTACTGCGA TTGCGGTCTG 
AAGTGGTTCT CCGATTGGAT AAAGCTGGAC TACGTGGAAC CGGGAATTGC ACGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACAC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGCAACG CCTGTTTTGA GCAGCCGTGC 
CAGAATCAAG CGCAGTGCGT GGCCCTTCCG CAGCGAGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG TGAGTTTATG ATCGATGCTT GCTACGGAAA TCCGTGCCGC 
AACAATGCCA CTTGCACGGT GCTGGAGGAG GGTCGTTTCA GCTGTCAGTG CGCTCCGGGA 
TACACAGGTG CCCGCTGCGA GACGAATATC GACGATTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CCTGCATCGA CGGAGTGGAG TCGTACAAAT GTGAGTGCCA GCCGGGATTC 
AGTGGCGAGT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CGGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATGGA CCACTTTACC CACTACAGCT GCGATTGTCA GGCAGGTTTC 
CATGGCACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGT 
GGAACGTGCG TGGACGGCAT CAACGACTAC CAATGCCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGCCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGTCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGTTA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATAAGCTTT 
GTCCACAACA ACTCGTTTGT AGAACTGGAG CCACTGCGAA CACGTCCGGA GGCGAACGTG 
ACGATAGTCT TCAGTAGCGC GGAGCAGAAT GGAATTCTCA TGTACGACGG CCAGGATGCT 
CATCTTGCAG TGGAACTGTT TAATGGGCGT ATTCGGGTTA GCTACGATGT GGGTAATCAC 
CCTGTGTCCA CGATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCCGTGGAG 
CTTCTGGCCA TCAAGAAGAA TTTCACGCTG CGCGTGGATC GCGGATTGGC CCGTTCCATC 
ATCAACGAGG GCTCCAACGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGCCTA 
CCCGTGGATC CTGCACAGCA GGCGTACAAG AACTGGCAAA TACGCAACCT TACCAGCTTT 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAT CATAAGCTGG TCGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC AGGATGTGCC CTGCTCGAAG GAGAGCAGCA AGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACA CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAATGCCG TCGGGGCAGT CGCTGTGTGC CGAATTCCAA TGCCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTACACGG AGAACGACTG TCGCTCGAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAA ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sli ( NM_001103864.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097334.1 Drosophila melanogaster slit (sli), transcript variant E, mRNA.

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CloneID	OFa10126
Clone ID Related Accession (Same CDS sequence)	NM_057379.4 , NM_001103864.3
Accession Version	NM_001103864.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slit, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103864.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT TGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACGACCGT GGGTAGACGC GTCTTCAAGG GAGCCCAATC GTTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGC CTGGATGAGC ACGCCTTTAA GGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAACAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTTTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCGCG ATTCCTTCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGTGCGGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACTT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAATTT CATTACGGAA 
CTGCCGCCGA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATATCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGCTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTTCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCCATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACAACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGTCGCC TGCCGCACTT GGTGAAGCTG 
GAATTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG AGCATCCCAC 
ATCCAGGAGT TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TATCGAACAA GATGTTCCTG 
GGACTGCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCATG CGTTATGCCC 
GGTTCCTTTG AGCATCTCAA CTCTCTGACG TCGCTGAACC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGCGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CTTGCCCCAC 
TCGGAATTCA AGTGTAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTC CGCTGTTCGC GTAACCAGCT GAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATACG CCATTTGCGC TCCCTTACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTT GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CGCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAC 
CTCAAGTCGT TGACCCACAT CGCACTAGGC AGCAATCCCT TGTACTGCGA TTGCGGTCTG 
AAGTGGTTCT CCGATTGGAT AAAGCTGGAC TACGTGGAAC CGGGAATTGC ACGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACAC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGCAACG CCTGTTTTGA GCAGCCGTGC 
CAGAATCAAG CGCAGTGCGT GGCCCTTCCG CAGCGAGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG TGAGTTTATG ATCGATGCTT GCTACGGAAA TCCGTGCCGC 
AACAATGCCA CTTGCACGGT GCTGGAGGAG GGTCGTTTCA GCTGTCAGTG CGCTCCGGGA 
TACACAGGTG CCCGCTGCGA GACGAATATC GACGATTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CCTGCATCGA CGGAGTGGAG TCGTACAAAT GTGAGTGCCA GCCGGGATTC 
AGTGGCGAGT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CGGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATGGA CCACTTTACC CACTACAGCT GCGATTGTCA GGCAGGTTTC 
CATGGCACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGT 
GGAACGTGCG TGGACGGCAT CAACGACTAC CAATGCCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGCCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGTCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGTTA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATAAGCTTT 
GTCCACAACA ACTCGTTTGT AGAACTGGAG CCACTGCGAA CACGTCCGGA GGCGAACGTG 
ACGATAGTCT TCAGTAGCGC GGAGCAGAAT GGAATTCTCA TGTACGACGG CCAGGATGCT 
CATCTTGCAG TGGAACTGTT TAATGGGCGT ATTCGGGTTA GCTACGATGT GGGTAATCAC 
CCTGTGTCCA CGATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCCGTGGAG 
CTTCTGGCCA TCAAGAAGAA TTTCACGCTG CGCGTGGATC GCGGATTGGC CCGTTCCATC 
ATCAACGAGG GCTCCAACGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGCCTA 
CCCGTGGATC CTGCACAGCA GGCGTACAAG AACTGGCAAA TACGCAACCT TACCAGCTTT 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAT CATAAGCTGG TCGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC AGGATGTGCC CTGCTCGAAG GAGAGCAGCA AGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACA CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAATGCCG TCGGGGCAGT CGCTGTGTGC CGAATTCCAA TGCCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTACACGG AGAACGACTG TCGCTCGAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAA ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097334.1
CDS	315..4829
Translation	MAAPSRTTLMPPPFRLQLRLLILPILLLLRHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY

Target ORF information:

RefSeq Version	NM_001103864.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slit (sli), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103864.3

ORF Insert Sequence:

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT TGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACGACCGT GGGTAGACGC GTCTTCAAGG GAGCCCAATC GTTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGC CTGGATGAGC ACGCCTTTAA GGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAACAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTTTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCGCG ATTCCTTCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGTGCGGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACTT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAATTT CATTACGGAA 
CTGCCGCCGA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATATCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGCTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTTCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCCATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACAACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGTCGCC TGCCGCACTT GGTGAAGCTG 
GAATTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG AGCATCCCAC 
ATCCAGGAGT TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TATCGAACAA GATGTTCCTG 
GGACTGCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCATG CGTTATGCCC 
GGTTCCTTTG AGCATCTCAA CTCTCTGACG TCGCTGAACC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGCGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CTTGCCCCAC 
TCGGAATTCA AGTGTAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTC CGCTGTTCGC GTAACCAGCT GAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATACG CCATTTGCGC TCCCTTACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTT GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CGCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAC 
CTCAAGTCGT TGACCCACAT CGCACTAGGC AGCAATCCCT TGTACTGCGA TTGCGGTCTG 
AAGTGGTTCT CCGATTGGAT AAAGCTGGAC TACGTGGAAC CGGGAATTGC ACGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACAC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGCAACG CCTGTTTTGA GCAGCCGTGC 
CAGAATCAAG CGCAGTGCGT GGCCCTTCCG CAGCGAGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG TGAGTTTATG ATCGATGCTT GCTACGGAAA TCCGTGCCGC 
AACAATGCCA CTTGCACGGT GCTGGAGGAG GGTCGTTTCA GCTGTCAGTG CGCTCCGGGA 
TACACAGGTG CCCGCTGCGA GACGAATATC GACGATTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CCTGCATCGA CGGAGTGGAG TCGTACAAAT GTGAGTGCCA GCCGGGATTC 
AGTGGCGAGT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CGGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATGGA CCACTTTACC CACTACAGCT GCGATTGTCA GGCAGGTTTC 
CATGGCACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGT 
GGAACGTGCG TGGACGGCAT CAACGACTAC CAATGCCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGCCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGTCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGTTA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATAAGCTTT 
GTCCACAACA ACTCGTTTGT AGAACTGGAG CCACTGCGAA CACGTCCGGA GGCGAACGTG 
ACGATAGTCT TCAGTAGCGC GGAGCAGAAT GGAATTCTCA TGTACGACGG CCAGGATGCT 
CATCTTGCAG TGGAACTGTT TAATGGGCGT ATTCGGGTTA GCTACGATGT GGGTAATCAC 
CCTGTGTCCA CGATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCCGTGGAG 
CTTCTGGCCA TCAAGAAGAA TTTCACGCTG CGCGTGGATC GCGGATTGGC CCGTTCCATC 
ATCAACGAGG GCTCCAACGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGCCTA 
CCCGTGGATC CTGCACAGCA GGCGTACAAG AACTGGCAAA TACGCAACCT TACCAGCTTT 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAT CATAAGCTGG TCGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC AGGATGTGCC CTGCTCGAAG GAGAGCAGCA AGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACA CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAATGCCG TCGGGGCAGT CGCTGTGTGC CGAATTCCAA TGCCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTACACGG AGAACGACTG TCGCTCGAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAA ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sli ( NM_057381.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476729.1 Drosophila melanogaster slit (sli), transcript variant B, mRNA.

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CloneID	OFa10127
Clone ID Related Accession (Same CDS sequence)	NM_057381.4
Accession Version	NM_057381.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slit, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_057381.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTGG ACATCTCCAA CAATGTCATC 
ACGACCGTGG GTAGACGCGT CTTCAAGGGA GCCCAATCGT TGCGGAGTCT TCAGCTGGAC 
AATAACCAAA TCACCTGCCT GGATGAGCAC GCCTTTAAGG GATTGGTGGA GCTGGAGATA 
CTCACGCTGA ACAACAACAA CCTGACTTCC CTGCCGCACA ACATCTTCGG CGGACTGGGA 
CGTTTGCGGG CACTCCGGCT GTCGGACAAT CCGTTCGCCT GCGACTGCCA TCTGTCCTGG 
CTGTCGCGAT TCCTTCGCAG TGCCACCCGC CTGGCGCCCT ACACCCGCTG CCAGTCGCCA 
TCGCAGCTGA AGGGCCAAAA CGTGGCGGAC CTGCACGACC AGGAGTTCAA ATGCTCGGGT 
CTGACGGAGC ACGCACCGAT GGAATGCGGG GCGGAGAACA GCTGTCCGCA CCCATGTCGC 
TGTGCGGACG GGATCGTCGA TTGCCGTGAG AAGAGTCTGA CCAGCGTGCC CGTCACTTTG 
CCCGACGACA CCACCGAGCT CCGCCTCGAG CAGAATTTCA TTACGGAACT GCCGCCGAAA 
TCGTTCTCCA GCTTTCGACG ACTGCGACGC ATCGACCTGT CCAACAACAA CATATCCCGG 
ATTGCCCACG ATGCACTAAG CGGCCTAAAG CAGTTAACCA CTCTCGTGCT GTACGGCAAT 
AAAATAAAGG ATTTACCCTC GGGCGTGTTC AAAGGACTCG GCTCGCTGCA GCTGCTGCTG 
CTGAACGCCA ACGAGATCTC GTGCATACGC AAGGATGCCT TTCGCGACCT GCACAGTTTG 
AGCCTGCTCT CCCTGTACGA CAACAACATC CAGTCGCTGG CTAATGGCAC ATTCGACGCC 
ATGAAGAGCA TCAAAACGGT ACATCTGGCC AAGAATCCTT TCATCTGCGA CTGCAATCTG 
CGCTGGCTGG CCGACTATTT GCACAAAAAT CCCATAGAGA CGAGTGGAGC CCGCTGCGAG 
TCACCGAAGC GGATGCATCG TCGTCGGATT GAATCGCTGC GCGAGGAGAA ATTCAAATGC 
TCCTGGGATG AATTGCGGAT GAAGCTGTCC GGCGAGTGCC GCATGGACTC CGACTGTCCG 
GCCATGTGCC ACTGCGAGGG CACCACCGTG GATTGCACGG GCCGGGGCCT GAAGGAGATT 
CCGCGCGACA TTCCCCTGCA CACAACTGAG CTTTTGCTCA ACGACAACGA ACTGGGACGC 
ATCAGTTCCG ATGGCCTCTT TGGTCGCCTG CCGCACTTGG TGAAGCTGGA ATTGAAGCGC 
AACCAGCTGA CCGGCATCGA GCCCAACGCC TTCGAGGGAG CATCCCACAT CCAGGAGTTG 
CAGCTGGGCG AGAACAAGAT CAAGGAGATA TCGAACAAGA TGTTCCTGGG ACTGCACCAA 
CTAAAAACGC TCAATCTGTA CGACAATCAA ATCTCATGCG TTATGCCCGG TTCCTTTGAG 
CATCTCAACT CTCTGACGTC GCTGAACCTC GCATCGAATC CATTCAATTG CAATTGTCAT 
TTGGCCTGGT TCGCGGAATG GCTGCGCAAA AAATCACTGA ACGGCGGAGC GGCACGTTGT 
GGAGCCCCGT CGAAGGTACG TGACGTGCAG ATCAAGGACT TGCCCCACTC GGAATTCAAG 
TGTAGCAGCG AGAACAGCGA GGGCTGCCTG GGCGATGGCT ACTGTCCGCC ATCCTGCACC 
TGCACCGGCA CCGTGGTCCG CTGTTCGCGT AACCAGCTGA AGGAGATACC GCGCGGCATT 
CCCGCCGAGA CATCGGAGCT GTATCTGGAG TCCAATGAGA TCGAGCAGAT TCACTACGAA 
CGCATACGCC ATTTGCGCTC CCTTACCCGA CTCGATCTCA GCAACAACCA GATCACCATT 
CTCTCCAACT ACACCTTTGC CAATCTGACC AAGCTGTCCA CGCTCATCAT CTCATACAAC 
AAGCTGCAGT GTCTGCAGCG GCATGCGTTG TCTGGCCTGA ATAACCTGCG CGTGCTTTCG 
CTGCACGGTA ACCGCATCTC GATGCTGCCG GAAGGCTCCT TCGAGGACCT CAAGTCGTTG 
ACCCACATCG CACTAGGCAG CAATCCCTTG TACTGCGATT GCGGTCTGAA GTGGTTCTCC 
GATTGGATAA AGCTGGACTA CGTGGAACCG GGAATTGCAC GTTGCGCCGA ACCGGAACAG 
ATGAAGGATA AGCTGATCCT GTCCACACCC TCGTCGAGCT TCGTTTGCCG CGGCCGCGTG 
CGCAATGATA TTCTGGCCAA GTGCAACGCC TGTTTTGAGC AGCCGTGCCA GAATCAAGCG 
CAGTGCGTGG CCCTTCCGCA GCGAGAGTAC CAGTGCCTCT GCCAGCCGGG CTATCATGGC 
AAACACTGTG AGTTTATGAT CGATGCTTGC TACGGAAATC CGTGCCGCAA CAATGCCACT 
TGCACGGTGC TGGAGGAGGG TCGTTTCAGC TGTCAGTGCG CTCCGGGATA CACAGGTGCC 
CGCTGCGAGA CGAATATCGA CGATTGCCTG GGCGAGATCA AGTGCCAGAA CAATGCCACC 
TGCATCGACG GAGTGGAGTC GTACAAATGT GAGTGCCAGC CGGGATTCAG TGGCGAGTTC 
TGCGACACCA AAATCCAGTT CTGCAGTCCG GAGTTCAATC CCTGCGCGAA TGGAGCCAAG 
TGCATGGACC ACTTTACCCA CTACAGCTGC GATTGTCAGG CAGGTTTCCA TGGCACCAAC 
TGCACGGACA ATATTGACGA CTGCCAGAAC CACATGTGCC AGAACGGTGG AACGTGCGTG 
GACGGCATCA ACGACTACCA ATGCCGCTGT CCAGACGACT ATACGGGCAA GTACTGTGAA 
GGCCACAACA TGATCTCGAT GATGTATCCA CAGACGTCGC CTTGTCAAAA CCACGAGTGC 
AAGCACGGTG TCTGCTTCCA ACCGAACGCT CAGGGCAGCG ACTACCTATG CAGGTGTCAT 
CCGGGTTACA CTGGAAAGTG GTGCGAGTAC CTCACCAGCA TAAGCTTTGT CCACAACAAC 
TCGTTTGTAG AACTGGAGCC ACTGCGAACA CGTCCGGAGG CGAACGTGAC GATAGTCTTC 
AGTAGCGCGG AGCAGAATGG AATTCTCATG TACGACGGCC AGGATGCTCA TCTTGCAGTG 
GAACTGTTTA ATGGGCGTAT TCGGGTTAGC TACGATGTGG GTAATCACCC TGTGTCCACG 
ATGTACAGCT TTGAGATGGT GGCCGATGGA AAGTACCATG CCGTGGAGCT TCTGGCCATC 
AAGAAGAATT TCACGCTGCG CGTGGATCGC GGATTGGCCC GTTCCATCAT CAACGAGGGC 
TCCAACGACT ACCTGAAACT CACGACGCCC ATGTTCCTGG GCGGCCTACC CGTGGATCCT 
GCACAGCAGG CGTACAAGAA CTGGCAAATA CGCAACCTTA CCAGCTTTAA GGGCTGCATG 
AAGGAGGTGT GGATCAATCA TAAGCTGGTC GACTTTGGCA ATGCCCAGCG CCAGCAAAAG 
ATCACACCAG GATGTGCCCT GCTCGAAGGA GAGCAGCAAG AGGAGGAAGA CGACGAGCAG 
GATTTCATGG ACGAGACACC GCACATCAAA GAGGAGCCGG TGGATCCTTG CCTGGAGAAC 
AAATGCCGTC GGGGCAGTCG CTGTGTGCCG AATTCCAATG CCAGGGACGG CTACCAGTGC 
AAGTGCAAGC ACGGCCAGCG CGGCCGCTAC TGCGATCAAG CGGCGTCCAC CTGTCGCAAG 
GAGCAGGTGC GCGAGTACTA CACGGAGAAC GACTGTCGCT CGAGGCAGCC GTTGAAGTAC 
GCCAAGTGCG TGGGCGGCTG CGGCAACCAG TGCTGCGCGG CCAAAATTGT GAGACGGCGC 
AAGGTGCGCA TGGTGTGCAG CAACAACCGC AAGTACATCA AGAACTTGGA CATCGTGCGC 
AAGTGCGGAT GCACCAAGAA ATGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476729.1
CDS	1220..5629
Translation	MAAPSRTTLMPPPFRLQLRLLILPILLLLRHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARDGYQCKCKHGQRGRYCDQAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY

Target ORF information:

RefSeq Version	NM_057381.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slit (sli), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057381.4

ORF Insert Sequence:

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTGG ACATCTCCAA CAATGTCATC 
ACGACCGTGG GTAGACGCGT CTTCAAGGGA GCCCAATCGT TGCGGAGTCT TCAGCTGGAC 
AATAACCAAA TCACCTGCCT GGATGAGCAC GCCTTTAAGG GATTGGTGGA GCTGGAGATA 
CTCACGCTGA ACAACAACAA CCTGACTTCC CTGCCGCACA ACATCTTCGG CGGACTGGGA 
CGTTTGCGGG CACTCCGGCT GTCGGACAAT CCGTTCGCCT GCGACTGCCA TCTGTCCTGG 
CTGTCGCGAT TCCTTCGCAG TGCCACCCGC CTGGCGCCCT ACACCCGCTG CCAGTCGCCA 
TCGCAGCTGA AGGGCCAAAA CGTGGCGGAC CTGCACGACC AGGAGTTCAA ATGCTCGGGT 
CTGACGGAGC ACGCACCGAT GGAATGCGGG GCGGAGAACA GCTGTCCGCA CCCATGTCGC 
TGTGCGGACG GGATCGTCGA TTGCCGTGAG AAGAGTCTGA CCAGCGTGCC CGTCACTTTG 
CCCGACGACA CCACCGAGCT CCGCCTCGAG CAGAATTTCA TTACGGAACT GCCGCCGAAA 
TCGTTCTCCA GCTTTCGACG ACTGCGACGC ATCGACCTGT CCAACAACAA CATATCCCGG 
ATTGCCCACG ATGCACTAAG CGGCCTAAAG CAGTTAACCA CTCTCGTGCT GTACGGCAAT 
AAAATAAAGG ATTTACCCTC GGGCGTGTTC AAAGGACTCG GCTCGCTGCA GCTGCTGCTG 
CTGAACGCCA ACGAGATCTC GTGCATACGC AAGGATGCCT TTCGCGACCT GCACAGTTTG 
AGCCTGCTCT CCCTGTACGA CAACAACATC CAGTCGCTGG CTAATGGCAC ATTCGACGCC 
ATGAAGAGCA TCAAAACGGT ACATCTGGCC AAGAATCCTT TCATCTGCGA CTGCAATCTG 
CGCTGGCTGG CCGACTATTT GCACAAAAAT CCCATAGAGA CGAGTGGAGC CCGCTGCGAG 
TCACCGAAGC GGATGCATCG TCGTCGGATT GAATCGCTGC GCGAGGAGAA ATTCAAATGC 
TCCTGGGATG AATTGCGGAT GAAGCTGTCC GGCGAGTGCC GCATGGACTC CGACTGTCCG 
GCCATGTGCC ACTGCGAGGG CACCACCGTG GATTGCACGG GCCGGGGCCT GAAGGAGATT 
CCGCGCGACA TTCCCCTGCA CACAACTGAG CTTTTGCTCA ACGACAACGA ACTGGGACGC 
ATCAGTTCCG ATGGCCTCTT TGGTCGCCTG CCGCACTTGG TGAAGCTGGA ATTGAAGCGC 
AACCAGCTGA CCGGCATCGA GCCCAACGCC TTCGAGGGAG CATCCCACAT CCAGGAGTTG 
CAGCTGGGCG AGAACAAGAT CAAGGAGATA TCGAACAAGA TGTTCCTGGG ACTGCACCAA 
CTAAAAACGC TCAATCTGTA CGACAATCAA ATCTCATGCG TTATGCCCGG TTCCTTTGAG 
CATCTCAACT CTCTGACGTC GCTGAACCTC GCATCGAATC CATTCAATTG CAATTGTCAT 
TTGGCCTGGT TCGCGGAATG GCTGCGCAAA AAATCACTGA ACGGCGGAGC GGCACGTTGT 
GGAGCCCCGT CGAAGGTACG TGACGTGCAG ATCAAGGACT TGCCCCACTC GGAATTCAAG 
TGTAGCAGCG AGAACAGCGA GGGCTGCCTG GGCGATGGCT ACTGTCCGCC ATCCTGCACC 
TGCACCGGCA CCGTGGTCCG CTGTTCGCGT AACCAGCTGA AGGAGATACC GCGCGGCATT 
CCCGCCGAGA CATCGGAGCT GTATCTGGAG TCCAATGAGA TCGAGCAGAT TCACTACGAA 
CGCATACGCC ATTTGCGCTC CCTTACCCGA CTCGATCTCA GCAACAACCA GATCACCATT 
CTCTCCAACT ACACCTTTGC CAATCTGACC AAGCTGTCCA CGCTCATCAT CTCATACAAC 
AAGCTGCAGT GTCTGCAGCG GCATGCGTTG TCTGGCCTGA ATAACCTGCG CGTGCTTTCG 
CTGCACGGTA ACCGCATCTC GATGCTGCCG GAAGGCTCCT TCGAGGACCT CAAGTCGTTG 
ACCCACATCG CACTAGGCAG CAATCCCTTG TACTGCGATT GCGGTCTGAA GTGGTTCTCC 
GATTGGATAA AGCTGGACTA CGTGGAACCG GGAATTGCAC GTTGCGCCGA ACCGGAACAG 
ATGAAGGATA AGCTGATCCT GTCCACACCC TCGTCGAGCT TCGTTTGCCG CGGCCGCGTG 
CGCAATGATA TTCTGGCCAA GTGCAACGCC TGTTTTGAGC AGCCGTGCCA GAATCAAGCG 
CAGTGCGTGG CCCTTCCGCA GCGAGAGTAC CAGTGCCTCT GCCAGCCGGG CTATCATGGC 
AAACACTGTG AGTTTATGAT CGATGCTTGC TACGGAAATC CGTGCCGCAA CAATGCCACT 
TGCACGGTGC TGGAGGAGGG TCGTTTCAGC TGTCAGTGCG CTCCGGGATA CACAGGTGCC 
CGCTGCGAGA CGAATATCGA CGATTGCCTG GGCGAGATCA AGTGCCAGAA CAATGCCACC 
TGCATCGACG GAGTGGAGTC GTACAAATGT GAGTGCCAGC CGGGATTCAG TGGCGAGTTC 
TGCGACACCA AAATCCAGTT CTGCAGTCCG GAGTTCAATC CCTGCGCGAA TGGAGCCAAG 
TGCATGGACC ACTTTACCCA CTACAGCTGC GATTGTCAGG CAGGTTTCCA TGGCACCAAC 
TGCACGGACA ATATTGACGA CTGCCAGAAC CACATGTGCC AGAACGGTGG AACGTGCGTG 
GACGGCATCA ACGACTACCA ATGCCGCTGT CCAGACGACT ATACGGGCAA GTACTGTGAA 
GGCCACAACA TGATCTCGAT GATGTATCCA CAGACGTCGC CTTGTCAAAA CCACGAGTGC 
AAGCACGGTG TCTGCTTCCA ACCGAACGCT CAGGGCAGCG ACTACCTATG CAGGTGTCAT 
CCGGGTTACA CTGGAAAGTG GTGCGAGTAC CTCACCAGCA TAAGCTTTGT CCACAACAAC 
TCGTTTGTAG AACTGGAGCC ACTGCGAACA CGTCCGGAGG CGAACGTGAC GATAGTCTTC 
AGTAGCGCGG AGCAGAATGG AATTCTCATG TACGACGGCC AGGATGCTCA TCTTGCAGTG 
GAACTGTTTA ATGGGCGTAT TCGGGTTAGC TACGATGTGG GTAATCACCC TGTGTCCACG 
ATGTACAGCT TTGAGATGGT GGCCGATGGA AAGTACCATG CCGTGGAGCT TCTGGCCATC 
AAGAAGAATT TCACGCTGCG CGTGGATCGC GGATTGGCCC GTTCCATCAT CAACGAGGGC 
TCCAACGACT ACCTGAAACT CACGACGCCC ATGTTCCTGG GCGGCCTACC CGTGGATCCT 
GCACAGCAGG CGTACAAGAA CTGGCAAATA CGCAACCTTA CCAGCTTTAA GGGCTGCATG 
AAGGAGGTGT GGATCAATCA TAAGCTGGTC GACTTTGGCA ATGCCCAGCG CCAGCAAAAG 
ATCACACCAG GATGTGCCCT GCTCGAAGGA GAGCAGCAAG AGGAGGAAGA CGACGAGCAG 
GATTTCATGG ACGAGACACC GCACATCAAA GAGGAGCCGG TGGATCCTTG CCTGGAGAAC 
AAATGCCGTC GGGGCAGTCG CTGTGTGCCG AATTCCAATG CCAGGGACGG CTACCAGTGC 
AAGTGCAAGC ACGGCCAGCG CGGCCGCTAC TGCGATCAAG CGGCGTCCAC CTGTCGCAAG 
GAGCAGGTGC GCGAGTACTA CACGGAGAAC GACTGTCGCT CGAGGCAGCC GTTGAAGTAC 
GCCAAGTGCG TGGGCGGCTG CGGCAACCAG TGCTGCGCGG CCAAAATTGT GAGACGGCGC 
AAGGTGCGCA TGGTGTGCAG CAACAACCGC AAGTACATCA AGAACTTGGA CATCGTGCGC 
AAGTGCGGAT GCACCAAGAA ATGCTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sli ( NM_001274088.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261017.1 Drosophila melanogaster slit (sli), transcript variant F, mRNA.

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CloneID	OFa12089
Clone ID Related Accession (Same CDS sequence)	NM_001274088.1
Accession Version	NM_001274088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slit, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT TGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACGACCGT GGGTAGACGC GTCTTCAAGG GAGCCCAATC GTTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGC CTGGATGAGC ACGCCTTTAA GGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAACAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTTTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCGCG ATTCCTTCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGTGCGGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACTT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAATTT CATTACGGAA 
CTGCCGCCGA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATATCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGCTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTTCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCCATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACAACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGTCGCC TGCCGCACTT GGTGAAGCTG 
GAATTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG AGCATCCCAC 
ATCCAGGAGT TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TATCGAACAA GATGTTCCTG 
GGACTGCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCATG CGTTATGCCC 
GGTTCCTTTG AGCATCTCAA CTCTCTGACG TCGCTGAACC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGCGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CTTGCCCCAC 
TCGGAATTCA AGTGTAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTC CGCTGTTCGC GTAACCAGCT GAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATACG CCATTTGCGC TCCCTTACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTT GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CGCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAC 
CTCAAGTCGT TGACCCACAT CGCACTAGGC AGCAATCCCT TGTACTGCGA TTGCGGTCTG 
AAGTGGTTCT CCGATTGGAT AAAGCTGGAC TACGTGGAAC CGGGAATTGC ACGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACAC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGCAACG CCTGTTTTGA GCAGCCGTGC 
CAGAATCAAG CGCAGTGCGT GGCCCTTCCG CAGCGAGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG TGAGTTTATG ATCGATGCTT GCTACGGAAA TCCGTGCCGC 
AACAATGCCA CTTGCACGGT GCTGGAGGAG GGTCGTTTCA GCTGTCAGTG CGCTCCGGGA 
TACACAGGTG CCCGCTGCGA GACGAATATC GACGATTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CCTGCATCGA CGGAGTGGAG TCGTACAAAT GTGAGTGCCA GCCGGGATTC 
AGTGGCGAGT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CGGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATGGA CCACTTTACC CACTACAGCT GCGATTGTCA GGCAGGTTTC 
CATGGCACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGT 
GGAACGTGCG TGGACGGCAT CAACGACTAC CAATGCCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGCCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGTCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGTTA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATAAGCTTT 
GTCCACAACA ACTCGTTTGT AGAACTGGAG CCACTGCGAA CACGTCCGGA GGCGAACGTG 
ACGATAGTCT TCAGTAGCGC GGAGCAGAAT GGAATTCTCA TGTACGACGG CCAGGATGCT 
CATCTTGCAG TGGAACTGTT TAATGGGCGT ATTCGGGTTA GCTACGATGT GGGTAATCAC 
CCTGTGTCCA CGATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCCGTGGAG 
CTTCTGGCCA TCAAGAAGAA TTTCACGCTG CGCGTGGATC GCGGATTGGC CCGTTCCATC 
ATCAACGAGG GCTCCAACGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGCCTA 
CCCGTGGATC CTGCACAGCA GGCGTACAAG AACTGGCAAA TACGCAACCT TACCAGCTTT 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAT CATAAGCTGG TCGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC AGGATGTGCC CTGCTCGAAG GAGAGCAGCA AGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACA CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAATGCCG TCGGGGCAGT CGCTGTGTGC CGAATTCCAA TGCCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTACACGG AGAACGACTG TCGCTCGAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAA ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGACTGAA AGATGCGACT ACCCAATTGC TCGAACGGAG CAATAGCAGC 
TTAGATGTTA GTTTAGGAAC AGGTTTAAAT CTAACTTATA GTAGTAGTTA TAGTAACGAT 
AGTCTTAGCC ATAGCACTAG GGATAGCACG GATGTTAGGG GGACGAAGGA TGAAGCGGAG 
GAGAGTGCTG ACGCGGGGGA GACAACGGAG GAGGTGAGGG ACGAGAGTGA TGATTACCCC 
ACGGACGGCA TGCAGTCCGA TCTCTACGAC GACACCATCG ATGACGACGA TGACGATGGT 
CTCGATGATG ATTATGCAGA CGAGGAGGAT GGGGAGGAGG ATCCAGAGCA GCTTCCCGAT 
CCCAAAGGTC TGGTAAGTGT GCCAGATGAA GAGGAAGACA TGGGTTACGA CGAGGATGAC 
GAACGTATCG CCATGGAGCG GCCAAGAACG GTTAGACCCA GGCCCGACGA GGAGCATTTC 
CTTAACGAAG AGGGCAGTGG TTTCGGCGGC TTTCGATCGC GATTCCGGCC GAGCAATAGC 
TTCCGCGAGA CTCAGCTGGA GAATATCCGC AAGAAGCTGC TCACAGAAGC ACAGGCGGCT 
CCGGAAACGG CTGTTGCGGT GGCCGTGCCG AGCACTGCGA TAGATCTGCG CGAGAGCAGC 
GGCCACTTTG CCAACGATGA CGAGGATGGC GAGGACGGCG ATGACGGCGT CGATGACGAG 
TTCGCCGACA CGGGGGAGAA CCAGGGGCGC GGCTTCTTTG GCTCCCAGCA GCAACAGCGC 
AAGAACGGTC CGTATCACCG CAAGAACGGC AACGATGCCA TCAAAATCAT CTCCACGCCG 
CTGGGCAAGG TGAGCATTGT GTACCAGCAG ACGGACAAGG ATCAAAGTCC GGACAAGGAT 
GCGCAACAGC AGCAGCAAAA GAAGCCGGCG CTCACCGACT TCGACGCCCT GTCACCGGAC 
CCCGAGAGCA GTCATCGCTT TCCGTCGCCC CACCCCAAGA TTACACCTGT TCTGACGCCC 
GATGGCAAGG TGGCGCTGCT CTATCGCGGA GACTCGGAGA GCTCCAAGTA CGAGCCCATA 
CGCAACCTGA CGCACAAGTT TTCGGGACAG CCGGCCAAGG AGTCAAAGCC TAAAACCGAA 
GATTCCTCCT CGGCGGAGGA CTCTGTCTAC ACGGACAGCG AGGATACCGA GGACAGTAAA 
GGTGAAACTA GTGCTGGAAA GTCCTCACCA GTGGCCTCCA CGCCCAAGCC ACTGCAGCCA 
GAGATTTTGG AGCCTCCAGA TAACGTCCAG CCAGGAGGGT CATTTATAAT TCGGCCCACC 
TCCGACTCGC TTCTGCCGAT GATCAACAGG CCACTGTCCG AGGTTCTGGG CATCAAGAAG 
AACCAGTTCC AGGAGACCCG GGTGCGTGAC CAATTGCCCA CGCAACAGCC TCCGCCTCCA 
CTGCCGGAGG CCACGTCCCG TAGTCCCGCT TCCGGACATC AGTTCCTAGC CAAGGTGAAC 
CTGGCTGAGT TCCCGACATC CGGAAGGACG CTCCAGACGC CGCTGATCCC CAGCACCCAC 
GACTTTGACT TCAGCCGTGA CAACACGATG CTGGACGAGC GGTCGCGGGT GCGTGAGTTG 
GAAAAGCAGC GGGAGCGGGA CAAGGAGCAC AACGAGGCCA CCAGCAAGGG AGCCACCGAG 
GCACACACCA TAGCCAACGA GCAGCTGCTC TCCAAGACGG AAGTCATCAA TCTGGCCATT 
GTGCCACAGT TTGACGAGGA CTTGGAGCGT CTACAGCGGC TGCAGGAGAA TGGTGGTCGG 
CGCCACCATA GGGCGCGACA TCGTCATCGG CAACAGTCGG AGGAGGAACT GTCTGGCATC 
CATTGCATCA TGCAGGTCAT GATGGGCGTG GCCGCCGTGT CGACGGTCTT CGGCATGCTG 
GGCACCTTCT TCAAGCAACG CATCCTCGAT CAGCTGCGCA TGATGCACTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261017.1
CDS	1158..7631
Translation	MAAPSRTTLMPPPFRLQLRLLILPILLLLRHDAVHAEPYSGGFGSSAVSSGGLGSVGIHIPGGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGRVRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTHYSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLTTPMFLGGLPVDPAQQAYKNWQIRNLTSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGEQQEEEDDEQDFMDETPHIKEEPVDPCLENKCRRGSRCVPNSNARDGYQCKCKHGQRGRYCDQGEGSTEPPTVTAASTCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKKCY*LKDATTQLLERSNSSLDVSLGTGLNLTYSSSYSNDSLSHSTRDSTDVRGTKDEAEESADAGETTEEVRDESDDYPTDGMQSDLYDDTIDDDDDDGLDDDYADEEDGEEDPEQLPDPKGLVSVPDEEEDMGYDEDDERIAMERPRTVRPRPDEEHFLNEEGSGFGGFRSRFRPSNSFRETQLENIRKKLLTEAQAAPETAVAVAVPSTAIDLRESSGHFANDDEDGEDGDDGVDDEFADTGENQGRGFFGSQQQQRKNGPYHRKNGNDAIKIISTPLGKVSIVYQQTDKDQSPDKDAQQQQQKKPALTDFDALSPDPESSHRFPSPHPKITPVLTPDGKVALLYRGDSESSKYEPIRNLTHKFSGQPAKESKPKTEDSSSAEDSVYTDSEDTEDSKGETSAGKSSPVASTPKPLQPEILEPPDNVQPGGSFIIRPTSDSLLPMINRPLSEVLGIKKNQFQETRVRDQLPTQQPPPPLPEATSRSPASGHQFLAKVNLAEFPTSGRTLQTPLIPSTHDFDFSRDNTMLDERSRVRELEKQRERDKEHNEATSKGATEAHTIANEQLLSKTEVINLAIVPQFDEDLERLQRLQENGGRRHHRARHRHRQQSEEELSGIHCIMQVMMGVAAVSTVFGMLGTFFKQRILDQLRMMHW

Target ORF information:

RefSeq Version	NM_001274088.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slit (sli), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274088.1

ORF Insert Sequence:

ATGGCCGCGC CGTCCAGGAC GACGTTGATG CCACCACCCT TCCGGCTCCA GCTGCGGCTA 
CTGATACTAC CCATCCTGCT ACTCCTGCGC CATGATGCGG TCCACGCGGA ACCGTATTCC 
GGCGGATTCG GCAGCTCAGC TGTATCCAGC GGTGGACTGG GGTCAGTGGG CATTCACATA 
CCCGGCGGCG GAGTGGGCGT CATCACGGAG GCCCGCTGCC CGAGGGTCTG CTCCTGCACC 
GGATTAAATG TGGATTGCTC GCATCGAGGA CTCACCTCCG TTCCCAGGAA AATCTCAGCG 
GACGTGGAGC GACTCGAGCT GCAGGGAAAC AATTTGACCG TGATATACGA GACGGATTTC 
CAGCGGCTGA CCAAGCTGCG AATGCTCCAA CTAACTGACA ATCAGATCCA CACGATCGAG 
AGGAACTCCT TCCAAGATTT GGTCTCACTC GAGCGACTAC GCCTAAACAA CAATCGACTA 
AAGGCAATTC CTGAAAACTT TGTGACAAGT TCAGCGAGTC TTTTGCGATT GGACATCTCC 
AACAATGTCA TCACGACCGT GGGTAGACGC GTCTTCAAGG GAGCCCAATC GTTGCGGAGT 
CTTCAGCTGG ACAATAACCA AATCACCTGC CTGGATGAGC ACGCCTTTAA GGGATTGGTG 
GAGCTGGAGA TACTCACGCT GAACAACAAC AACCTGACTT CCCTGCCGCA CAACATCTTC 
GGCGGACTGG GACGTTTGCG GGCACTCCGG CTGTCGGACA ATCCGTTCGC CTGCGACTGC 
CATCTGTCCT GGCTGTCGCG ATTCCTTCGC AGTGCCACCC GCCTGGCGCC CTACACCCGC 
TGCCAGTCGC CATCGCAGCT GAAGGGCCAA AACGTGGCGG ACCTGCACGA CCAGGAGTTC 
AAATGCTCGG GTCTGACGGA GCACGCACCG ATGGAATGCG GGGCGGAGAA CAGCTGTCCG 
CACCCATGTC GCTGTGCGGA CGGGATCGTC GATTGCCGTG AGAAGAGTCT GACCAGCGTG 
CCCGTCACTT TGCCCGACGA CACCACCGAG CTCCGCCTCG AGCAGAATTT CATTACGGAA 
CTGCCGCCGA AATCGTTCTC CAGCTTTCGA CGACTGCGAC GCATCGACCT GTCCAACAAC 
AACATATCCC GGATTGCCCA CGATGCACTA AGCGGCCTAA AGCAGTTAAC CACTCTCGTG 
CTGTACGGCA ATAAAATAAA GGATTTACCC TCGGGCGTGT TCAAAGGACT CGGCTCGCTG 
CAGCTGCTGC TGCTGAACGC CAACGAGATC TCGTGCATAC GCAAGGATGC CTTTCGCGAC 
CTGCACAGTT TGAGCCTGCT CTCCCTGTAC GACAACAACA TCCAGTCGCT GGCTAATGGC 
ACATTCGACG CCATGAAGAG CATCAAAACG GTACATCTGG CCAAGAATCC TTTCATCTGC 
GACTGCAATC TGCGCTGGCT GGCCGACTAT TTGCACAAAA ATCCCATAGA GACGAGTGGA 
GCCCGCTGCG AGTCACCGAA GCGGATGCAT CGTCGTCGGA TTGAATCGCT GCGCGAGGAG 
AAATTCAAAT GCTCCTGGGA TGAATTGCGG ATGAAGCTGT CCGGCGAGTG CCGCATGGAC 
TCCGACTGTC CGGCCATGTG CCACTGCGAG GGCACCACCG TGGATTGCAC GGGCCGGGGC 
CTGAAGGAGA TTCCGCGCGA CATTCCCCTG CACACAACTG AGCTTTTGCT CAACGACAAC 
GAACTGGGAC GCATCAGTTC CGATGGCCTC TTTGGTCGCC TGCCGCACTT GGTGAAGCTG 
GAATTGAAGC GCAACCAGCT GACCGGCATC GAGCCCAACG CCTTCGAGGG AGCATCCCAC 
ATCCAGGAGT TGCAGCTGGG CGAGAACAAG ATCAAGGAGA TATCGAACAA GATGTTCCTG 
GGACTGCACC AACTAAAAAC GCTCAATCTG TACGACAATC AAATCTCATG CGTTATGCCC 
GGTTCCTTTG AGCATCTCAA CTCTCTGACG TCGCTGAACC TCGCATCGAA TCCATTCAAT 
TGCAATTGTC ATTTGGCCTG GTTCGCGGAA TGGCTGCGCA AAAAATCACT GAACGGCGGA 
GCGGCACGTT GTGGAGCCCC GTCGAAGGTA CGTGACGTGC AGATCAAGGA CTTGCCCCAC 
TCGGAATTCA AGTGTAGCAG CGAGAACAGC GAGGGCTGCC TGGGCGATGG CTACTGTCCG 
CCATCCTGCA CCTGCACCGG CACCGTGGTC CGCTGTTCGC GTAACCAGCT GAAGGAGATA 
CCGCGCGGCA TTCCCGCCGA GACATCGGAG CTGTATCTGG AGTCCAATGA GATCGAGCAG 
ATTCACTACG AACGCATACG CCATTTGCGC TCCCTTACCC GACTCGATCT CAGCAACAAC 
CAGATCACCA TTCTCTCCAA CTACACCTTT GCCAATCTGA CCAAGCTGTC CACGCTCATC 
ATCTCATACA ACAAGCTGCA GTGTCTGCAG CGGCATGCGT TGTCTGGCCT GAATAACCTG 
CGCGTGCTTT CGCTGCACGG TAACCGCATC TCGATGCTGC CGGAAGGCTC CTTCGAGGAC 
CTCAAGTCGT TGACCCACAT CGCACTAGGC AGCAATCCCT TGTACTGCGA TTGCGGTCTG 
AAGTGGTTCT CCGATTGGAT AAAGCTGGAC TACGTGGAAC CGGGAATTGC ACGTTGCGCC 
GAACCGGAAC AGATGAAGGA TAAGCTGATC CTGTCCACAC CCTCGTCGAG CTTCGTTTGC 
CGCGGCCGCG TGCGCAATGA TATTCTGGCC AAGTGCAACG CCTGTTTTGA GCAGCCGTGC 
CAGAATCAAG CGCAGTGCGT GGCCCTTCCG CAGCGAGAGT ACCAGTGCCT CTGCCAGCCG 
GGCTATCATG GCAAACACTG TGAGTTTATG ATCGATGCTT GCTACGGAAA TCCGTGCCGC 
AACAATGCCA CTTGCACGGT GCTGGAGGAG GGTCGTTTCA GCTGTCAGTG CGCTCCGGGA 
TACACAGGTG CCCGCTGCGA GACGAATATC GACGATTGCC TGGGCGAGAT CAAGTGCCAG 
AACAATGCCA CCTGCATCGA CGGAGTGGAG TCGTACAAAT GTGAGTGCCA GCCGGGATTC 
AGTGGCGAGT TCTGCGACAC CAAAATCCAG TTCTGCAGTC CGGAGTTCAA TCCCTGCGCG 
AATGGAGCCA AGTGCATGGA CCACTTTACC CACTACAGCT GCGATTGTCA GGCAGGTTTC 
CATGGCACCA ACTGCACGGA CAATATTGAC GACTGCCAGA ACCACATGTG CCAGAACGGT 
GGAACGTGCG TGGACGGCAT CAACGACTAC CAATGCCGCT GTCCAGACGA CTATACGGGC 
AAGTACTGTG AAGGCCACAA CATGATCTCG ATGATGTATC CACAGACGTC GCCTTGTCAA 
AACCACGAGT GCAAGCACGG TGTCTGCTTC CAACCGAACG CTCAGGGCAG CGACTACCTA 
TGCAGGTGTC ATCCGGGTTA CACTGGAAAG TGGTGCGAGT ACCTCACCAG CATAAGCTTT 
GTCCACAACA ACTCGTTTGT AGAACTGGAG CCACTGCGAA CACGTCCGGA GGCGAACGTG 
ACGATAGTCT TCAGTAGCGC GGAGCAGAAT GGAATTCTCA TGTACGACGG CCAGGATGCT 
CATCTTGCAG TGGAACTGTT TAATGGGCGT ATTCGGGTTA GCTACGATGT GGGTAATCAC 
CCTGTGTCCA CGATGTACAG CTTTGAGATG GTGGCCGATG GAAAGTACCA TGCCGTGGAG 
CTTCTGGCCA TCAAGAAGAA TTTCACGCTG CGCGTGGATC GCGGATTGGC CCGTTCCATC 
ATCAACGAGG GCTCCAACGA CTACCTGAAA CTCACGACGC CCATGTTCCT GGGCGGCCTA 
CCCGTGGATC CTGCACAGCA GGCGTACAAG AACTGGCAAA TACGCAACCT TACCAGCTTT 
AAGGGCTGCA TGAAGGAGGT GTGGATCAAT CATAAGCTGG TCGACTTTGG CAATGCCCAG 
CGCCAGCAAA AGATCACACC AGGATGTGCC CTGCTCGAAG GAGAGCAGCA AGAGGAGGAA 
GACGACGAGC AGGATTTCAT GGACGAGACA CCGCACATCA AAGAGGAGCC GGTGGATCCT 
TGCCTGGAGA ACAAATGCCG TCGGGGCAGT CGCTGTGTGC CGAATTCCAA TGCCAGGGAC 
GGCTACCAGT GCAAGTGCAA GCACGGCCAG CGCGGCCGCT ACTGCGATCA AGGTGAGGGC 
AGCACTGAGC CCCCAACAGT CACCGCGGCG TCCACCTGTC GCAAGGAGCA GGTGCGCGAG 
TACTACACGG AGAACGACTG TCGCTCGAGG CAGCCGTTGA AGTACGCCAA GTGCGTGGGC 
GGCTGCGGCA ACCAGTGCTG CGCGGCCAAA ATTGTGAGAC GGCGCAAGGT GCGCATGGTG 
TGCAGCAACA ACCGCAAGTA CATCAAGAAC TTGGACATCG TGCGCAAGTG CGGATGCACC 
AAGAAATGCT ACTGACTGAA AGATGCGACT ACCCAATTGC TCGAACGGAG CAATAGCAGC 
TTAGATGTTA GTTTAGGAAC AGGTTTAAAT CTAACTTATA GTAGTAGTTA TAGTAACGAT 
AGTCTTAGCC ATAGCACTAG GGATAGCACG GATGTTAGGG GGACGAAGGA TGAAGCGGAG 
GAGAGTGCTG ACGCGGGGGA GACAACGGAG GAGGTGAGGG ACGAGAGTGA TGATTACCCC 
ACGGACGGCA TGCAGTCCGA TCTCTACGAC GACACCATCG ATGACGACGA TGACGATGGT 
CTCGATGATG ATTATGCAGA CGAGGAGGAT GGGGAGGAGG ATCCAGAGCA GCTTCCCGAT 
CCCAAAGGTC TGGTAAGTGT GCCAGATGAA GAGGAAGACA TGGGTTACGA CGAGGATGAC 
GAACGTATCG CCATGGAGCG GCCAAGAACG GTTAGACCCA GGCCCGACGA GGAGCATTTC 
CTTAACGAAG AGGGCAGTGG TTTCGGCGGC TTTCGATCGC GATTCCGGCC GAGCAATAGC 
TTCCGCGAGA CTCAGCTGGA GAATATCCGC AAGAAGCTGC TCACAGAAGC ACAGGCGGCT 
CCGGAAACGG CTGTTGCGGT GGCCGTGCCG AGCACTGCGA TAGATCTGCG CGAGAGCAGC 
GGCCACTTTG CCAACGATGA CGAGGATGGC GAGGACGGCG ATGACGGCGT CGATGACGAG 
TTCGCCGACA CGGGGGAGAA CCAGGGGCGC GGCTTCTTTG GCTCCCAGCA GCAACAGCGC 
AAGAACGGTC CGTATCACCG CAAGAACGGC AACGATGCCA TCAAAATCAT CTCCACGCCG 
CTGGGCAAGG TGAGCATTGT GTACCAGCAG ACGGACAAGG ATCAAAGTCC GGACAAGGAT 
GCGCAACAGC AGCAGCAAAA GAAGCCGGCG CTCACCGACT TCGACGCCCT GTCACCGGAC 
CCCGAGAGCA GTCATCGCTT TCCGTCGCCC CACCCCAAGA TTACACCTGT TCTGACGCCC 
GATGGCAAGG TGGCGCTGCT CTATCGCGGA GACTCGGAGA GCTCCAAGTA CGAGCCCATA 
CGCAACCTGA CGCACAAGTT TTCGGGACAG CCGGCCAAGG AGTCAAAGCC TAAAACCGAA 
GATTCCTCCT CGGCGGAGGA CTCTGTCTAC ACGGACAGCG AGGATACCGA GGACAGTAAA 
GGTGAAACTA GTGCTGGAAA GTCCTCACCA GTGGCCTCCA CGCCCAAGCC ACTGCAGCCA 
GAGATTTTGG AGCCTCCAGA TAACGTCCAG CCAGGAGGGT CATTTATAAT TCGGCCCACC 
TCCGACTCGC TTCTGCCGAT GATCAACAGG CCACTGTCCG AGGTTCTGGG CATCAAGAAG 
AACCAGTTCC AGGAGACCCG GGTGCGTGAC CAATTGCCCA CGCAACAGCC TCCGCCTCCA 
CTGCCGGAGG CCACGTCCCG TAGTCCCGCT TCCGGACATC AGTTCCTAGC CAAGGTGAAC 
CTGGCTGAGT TCCCGACATC CGGAAGGACG CTCCAGACGC CGCTGATCCC CAGCACCCAC 
GACTTTGACT TCAGCCGTGA CAACACGATG CTGGACGAGC GGTCGCGGGT GCGTGAGTTG 
GAAAAGCAGC GGGAGCGGGA CAAGGAGCAC AACGAGGCCA CCAGCAAGGG AGCCACCGAG 
GCACACACCA TAGCCAACGA GCAGCTGCTC TCCAAGACGG AAGTCATCAA TCTGGCCATT 
GTGCCACAGT TTGACGAGGA CTTGGAGCGT CTACAGCGGC TGCAGGAGAA TGGTGGTCGG 
CGCCACCATA GGGCGCGACA TCGTCATCGG CAACAGTCGG AGGAGGAACT GTCTGGCATC 
CATTGCATCA TGCAGGTCAT GATGGGCGTG GCCGCCGTGT CGACGGTCTT CGGCATGCTG 
GGCACCTTCT TCAAGCAACG CATCCTCGAT CAGCTGCGCA TGATGCACTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sli cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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