Slit2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Slit2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slit2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa58344 XM_017599269.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58345 XM_017599270.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58346 XM_017599271.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58347 XM_017599272.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58348 XM_017599273.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58349 XM_017599274.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58350 XM_017599275.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58351 XM_017599276.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58352 XM_017599277.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa58353 XM_017599278.1
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
ORa06182 NM_022632.2
Latest version!
Rattus norvegicus slit guidance ligand 2 (Slit2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa58344
    Clone ID Related Accession (Same CDS sequence) XM_017599269.1
    Accession Version XM_017599269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGAATTC ACCATTTGGC CTGTACAGAA
    GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAAAAATGT
    CGCTGTGAAG GGACCACAGT AGACTGCTCC AATCAAAAAC TCAACAAAAT CCCAGACCAT
    ATTCCCCAGT ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC
    ACGGGAATAT TTAAGAAACT TCCTCAATTG CGTAAAATCA ACCTTAGCAA CAATAAGATC
    ACTGATATCG AGGAGGGGGC ATTCGAAGGT GCGTCTGGTG TGAATGAGAT TCTGCTTACC
    AGTAACCGTT TGGAAAATGT TCAGCATAAG ATGTTCAAAG GATTGGAGAG CCTCAAAACA
    TTGATGCTGA GAAGTAATCG AATAAGCTGT GTGGGAAACG ACAGTTTCAC AGGACTCGGT
    TCTGTGCGTC TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTTGCACC AGGAGCATTT
    GGTACTCTCC ATTCATTATC TACACTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT
    CACCTGGCAT GGCTTGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA
    TGCCAAAAAC CCTACTTCTT GAAGGAAATA CCAATCCAGG ATGTAGCCAT TCAGGACTTC
    ACCTGTGATG ACGGAAACGA TGATAATAGC TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA
    TGTACTTGCT TGGATACAGT AGTACGATGT AGCAACAAGG GCTTGAAGGT CTTACCTAAA
    GGCATTCCAA GAGATGTCAC AGAACTGTAT CTGGATGGGA ACCAGTTTAC ACTGGTCCCG
    AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC
    ACCCTTTCCA ACCAAAGCTT CAGCAACATG ACCCAACTTC TCACCTTAAT TCTCAGTTAC
    AACCGTCTGA GATGTATCCC TCCACGGACC TTTGATGGAT TGAAATCTCT TCGTTTACTG
    TCTCTACATG GAAATGACAT TTCTGTCGTG CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC
    TTGTCACACT TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA
    TCCGACTGGG TGAAGTCGGA ATATAAGGAA CCTGGAATTG CCCGCTGTGC CGGTCCCGGA
    GAAATGGCAG ATAAATTGTT ACTCACAACT CCCTCCAAAA AATTTACATG TCAAGGGCTC
    CCAGGGCCCT GTGGCTTCCT AATCCCAGGT CCTGTGGATG TTACTATTCA AGCCAAGTGT
    AACCCCTGCT TGTCAAATCC ATGTAAAAAT GATGGCACCT GTAACAATGA CCCGGTGGAT
    TTTTATCGAT GCACCTGCCC ATATGGTTTC AAGGGCCAGG ACTGTGATGT CCCCATTCAT
    GCCTGTATCA GTAATCCATG TAAACATGGA GGAACTTGCC ACTTAAAAGA AGGAGAGAAT
    GATGGATTCT GGTGTACTTG TGCTGATGGG TTTGAAGGAG AAAGCTGTGA CATCAATATT
    GATGATTGCG AAGATAATGA TTGTGAAAAT AATTCTACAT GCGTTGATGG AATTAACAAC
    TACACGTGTC TTTGCCCACC GGAATACACA GCTGCTAATC TGAATGAGGT GGAAAAAGGC
    GAACTGTGTG AGGAAAAACT GGACTTCTGT GCACAAGACC TGAATCCCTG CCAGCATGAC
    TCCAAGTGCA TCCTGACGCC AAAGGGATTC AAGTGTGACT GCACTCCGGG ATACATTGGT
    GAGCACTGTG ACATCGACTT TGATGACTGC CAAGATAACA AGTGCAAAAA CGGTGCTCAT
    TGCACAGATG CAGTGAACGG ATACACATGT GTCTGTCCTG AAGGCTACAG TGGCTTGTTC
    TGTGAGTTTT CTCCACCCAT GGTCCTCCCT CGCACCAGCC CCTGTGATAA TTTTGATTGT
    CAGAATGGAG CCCAGTGTAT CATCAGGGTG AATGAACCAA TATGCCAGTG TTTGCCTGGC
    TACTTGGGAG AGAAGTGTGA GAAATTGGTC AGTGTGAATT TTGTAAACAA AGAGTCCTAT
    CTTCAGATTC CTTCAGCCAA GGTTCGACCT CAGACAAACA TCACACTTCA GATTGCCACA
    GATGAAGACA GCGGCATCCT CTTGTACAAG GGTGACAAGG ACCACATTGC TGTGGAACTC
    TATCGAGGGC GAGTTCGAGC CAGCTATGAC ACCGGCTCTC ACCCGGCTTC TGCCATTTAC
    AGTGTGGAGA CAATCAATGA TGGAAACTTC CACATTGTAG AGCTACTGAC CCTGGATTCG
    AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC CCTAAAATCA TCACCAATTT GTCAAAACAA
    TCTACTCTGA ATTTCGACTC TCCACTTTAC GTAGGAGGTA TGCCTGGGAA AAATAACGTG
    GCTTCGCTGC GCCAGGCCCC TGGGCAGAAC GGCACCAGCT TCCATGGCTG TATCCGGAAC
    CTTTACATTA ACAGTGAACT GCAGGACTTC CGGAAAGTGC CTATGCAAAC CGGAATTCTG
    CCTGGCTGTG AACCATGCCA CAAGAAAGTG TGTGCCCATG GCACATGCCA GCCCAGCAGC
    CAATCAGGCT TCACCTGTGA ATGTGAGGAA GGGTGGATGG GGCCCCTCTG TGACCAGAGA
    ACCAATGATC CCTGTCTCGG AAACAAATGT GTACATGGGA CCTGCTTGCC CATCAACGCC
    TTCTCCTACA GCTGCAAGTG CCTGGAGGGC CACGGCGGGG TCCTCTGTGA TGAAGAAGAA
    GATCTGTTTA ACCCCTGCCA GGTGATCAAG TGCAAGCACG GGAAGTGCAG GCTCTCTGGG
    CTCGGGCAGC CCTATTGTGA ATGCAGCAGT GGATTCACCG GGGACAGCTG TGACAGAGAA
    ATTTCTTGTC GAGGGGAACG GATAAGGGAT TATTACCAAA AGCAGCAGGG TTACGCTGCC
    TGTCAAACGA CTAAGAAAGT ATCTCGCTTG GAGTGCAGAG GCGGGTGTGC TGGGGGGCAG
    TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG AAATACTCTT TCGAATGCAC AGATGGATCT
    TCATTTGTGG ACGAGGTCGA GAAGGTGGTG AAGTGCGGCT GCACGAGATG TGCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454758.1
    CDS1650..6329
    Translation

    Target ORF information:

    RefSeq Version XM_017599269.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGAATTC ACCATTTGGC CTGTACAGAA
    GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAAAAATGT
    CGCTGTGAAG GGACCACAGT AGACTGCTCC AATCAAAAAC TCAACAAAAT CCCAGACCAT
    ATTCCCCAGT ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC
    ACGGGAATAT TTAAGAAACT TCCTCAATTG CGTAAAATCA ACCTTAGCAA CAATAAGATC
    ACTGATATCG AGGAGGGGGC ATTCGAAGGT GCGTCTGGTG TGAATGAGAT TCTGCTTACC
    AGTAACCGTT TGGAAAATGT TCAGCATAAG ATGTTCAAAG GATTGGAGAG CCTCAAAACA
    TTGATGCTGA GAAGTAATCG AATAAGCTGT GTGGGAAACG ACAGTTTCAC AGGACTCGGT
    TCTGTGCGTC TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTTGCACC AGGAGCATTT
    GGTACTCTCC ATTCATTATC TACACTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT
    CACCTGGCAT GGCTTGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA
    TGCCAAAAAC CCTACTTCTT GAAGGAAATA CCAATCCAGG ATGTAGCCAT TCAGGACTTC
    ACCTGTGATG ACGGAAACGA TGATAATAGC TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA
    TGTACTTGCT TGGATACAGT AGTACGATGT AGCAACAAGG GCTTGAAGGT CTTACCTAAA
    GGCATTCCAA GAGATGTCAC AGAACTGTAT CTGGATGGGA ACCAGTTTAC ACTGGTCCCG
    AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC
    ACCCTTTCCA ACCAAAGCTT CAGCAACATG ACCCAACTTC TCACCTTAAT TCTCAGTTAC
    AACCGTCTGA GATGTATCCC TCCACGGACC TTTGATGGAT TGAAATCTCT TCGTTTACTG
    TCTCTACATG GAAATGACAT TTCTGTCGTG CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC
    TTGTCACACT TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA
    TCCGACTGGG TGAAGTCGGA ATATAAGGAA CCTGGAATTG CCCGCTGTGC CGGTCCCGGA
    GAAATGGCAG ATAAATTGTT ACTCACAACT CCCTCCAAAA AATTTACATG TCAAGGGCTC
    CCAGGGCCCT GTGGCTTCCT AATCCCAGGT CCTGTGGATG TTACTATTCA AGCCAAGTGT
    AACCCCTGCT TGTCAAATCC ATGTAAAAAT GATGGCACCT GTAACAATGA CCCGGTGGAT
    TTTTATCGAT GCACCTGCCC ATATGGTTTC AAGGGCCAGG ACTGTGATGT CCCCATTCAT
    GCCTGTATCA GTAATCCATG TAAACATGGA GGAACTTGCC ACTTAAAAGA AGGAGAGAAT
    GATGGATTCT GGTGTACTTG TGCTGATGGG TTTGAAGGAG AAAGCTGTGA CATCAATATT
    GATGATTGCG AAGATAATGA TTGTGAAAAT AATTCTACAT GCGTTGATGG AATTAACAAC
    TACACGTGTC TTTGCCCACC GGAATACACA GCTGCTAATC TGAATGAGGT GGAAAAAGGC
    GAACTGTGTG AGGAAAAACT GGACTTCTGT GCACAAGACC TGAATCCCTG CCAGCATGAC
    TCCAAGTGCA TCCTGACGCC AAAGGGATTC AAGTGTGACT GCACTCCGGG ATACATTGGT
    GAGCACTGTG ACATCGACTT TGATGACTGC CAAGATAACA AGTGCAAAAA CGGTGCTCAT
    TGCACAGATG CAGTGAACGG ATACACATGT GTCTGTCCTG AAGGCTACAG TGGCTTGTTC
    TGTGAGTTTT CTCCACCCAT GGTCCTCCCT CGCACCAGCC CCTGTGATAA TTTTGATTGT
    CAGAATGGAG CCCAGTGTAT CATCAGGGTG AATGAACCAA TATGCCAGTG TTTGCCTGGC
    TACTTGGGAG AGAAGTGTGA GAAATTGGTC AGTGTGAATT TTGTAAACAA AGAGTCCTAT
    CTTCAGATTC CTTCAGCCAA GGTTCGACCT CAGACAAACA TCACACTTCA GATTGCCACA
    GATGAAGACA GCGGCATCCT CTTGTACAAG GGTGACAAGG ACCACATTGC TGTGGAACTC
    TATCGAGGGC GAGTTCGAGC CAGCTATGAC ACCGGCTCTC ACCCGGCTTC TGCCATTTAC
    AGTGTGGAGA CAATCAATGA TGGAAACTTC CACATTGTAG AGCTACTGAC CCTGGATTCG
    AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC CCTAAAATCA TCACCAATTT GTCAAAACAA
    TCTACTCTGA ATTTCGACTC TCCACTTTAC GTAGGAGGTA TGCCTGGGAA AAATAACGTG
    GCTTCGCTGC GCCAGGCCCC TGGGCAGAAC GGCACCAGCT TCCATGGCTG TATCCGGAAC
    CTTTACATTA ACAGTGAACT GCAGGACTTC CGGAAAGTGC CTATGCAAAC CGGAATTCTG
    CCTGGCTGTG AACCATGCCA CAAGAAAGTG TGTGCCCATG GCACATGCCA GCCCAGCAGC
    CAATCAGGCT TCACCTGTGA ATGTGAGGAA GGGTGGATGG GGCCCCTCTG TGACCAGAGA
    ACCAATGATC CCTGTCTCGG AAACAAATGT GTACATGGGA CCTGCTTGCC CATCAACGCC
    TTCTCCTACA GCTGCAAGTG CCTGGAGGGC CACGGCGGGG TCCTCTGTGA TGAAGAAGAA
    GATCTGTTTA ACCCCTGCCA GGTGATCAAG TGCAAGCACG GGAAGTGCAG GCTCTCTGGG
    CTCGGGCAGC CCTATTGTGA ATGCAGCAGT GGATTCACCG GGGACAGCTG TGACAGAGAA
    ATTTCTTGTC GAGGGGAACG GATAAGGGAT TATTACCAAA AGCAGCAGGG TTACGCTGCC
    TGTCAAACGA CTAAGAAAGT ATCTCGCTTG GAGTGCAGAG GCGGGTGTGC TGGGGGGCAG
    TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG AAATACTCTT TCGAATGCAC AGATGGATCT
    TCATTTGTGG ACGAGGTCGA GAAGGTGGTG AAGTGCGGCT GCACGAGATG TGCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58345
    Clone ID Related Accession (Same CDS sequence) XM_017599270.1
    Accession Version XM_017599270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC
    CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CGATTGCTTG TACCTGTAGC
    AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG TCATCCCTCC AGGAGCATTC
    TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA ACCAGATCTC GGAACTTGCT
    CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG TCCTGTATGG AAATAAAATC
    ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTACT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT
    CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG GCACCTTCTC AGCTCTCAGA
    GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG
    CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGTCCC
    CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAGCAGT ATTTCATTCC AGGAATTCAC CATTTGGCCT GTACAGAAGA TTATCGATCA
    AAATTAAGTG GAGACTGCTT TGCAGACTTG GCTTGTCCTG AAAAATGTCG CTGTGAAGGG
    ACCACAGTAG ACTGCTCCAA TCAAAAACTC AACAAAATCC CAGACCATAT TCCCCAGTAC
    ACAGCAGAGC TGCGTCTCAA TAATAATGAA TTCACAGTGT TAGAAGCCAC GGGAATATTT
    AAGAAACTTC CTCAATTGCG TAAAATCAAC CTTAGCAACA ATAAGATCAC TGATATCGAG
    GAGGGGGCAT TCGAAGGTGC GTCTGGTGTG AATGAGATTC TGCTTACCAG TAACCGTTTG
    GAAAATGTTC AGCATAAGAT GTTCAAAGGA TTGGAGAGCC TCAAAACATT GATGCTGAGA
    AGTAATCGAA TAAGCTGTGT GGGAAACGAC AGTTTCACAG GACTCGGTTC TGTGCGTCTG
    CTCTCTTTAT ATGACAATCA AATTACCACA GTTGCACCAG GAGCATTTGG TACTCTCCAT
    TCATTATCTA CACTAAACCT CTTGGCCAAT CCTTTCAACT GTAACTGTCA CCTGGCATGG
    CTTGGAGAAT GGCTCAGAAG GAAAAGAATT GTAACAGGAA ATCCTCGATG CCAAAAACCC
    TACTTCTTGA AGGAAATACC AATCCAGGAT GTAGCCATTC AGGACTTCAC CTGTGATGAC
    GGAAACGATG ATAATAGCTG CTCTCCACTC TCCCGTTGTC CTTCGGAATG TACTTGCTTG
    GATACAGTAG TACGATGTAG CAACAAGGGC TTGAAGGTCT TACCTAAAGG CATTCCAAGA
    GATGTCACAG AACTGTATCT GGATGGGAAC CAGTTTACAC TGGTCCCGAA GGAACTCTCC
    AACTACAAAC ATTTAACACT TATAGACTTA AGTAACAACA GAATAAGCAC CCTTTCCAAC
    CAAAGCTTCA GCAACATGAC CCAACTTCTC ACCTTAATTC TCAGTTACAA CCGTCTGAGA
    TGTATCCCTC CACGGACCTT TGATGGATTG AAATCTCTTC GTTTACTGTC TCTACATGGA
    AATGACATTT CTGTCGTGCC TGAAGGTGCC TTTGGTGACC TTTCAGCCTT GTCACACTTA
    GCAATTGGAG CCAACCCTCT TTACTGTGAT TGTAACATGC AGTGGTTATC CGACTGGGTG
    AAGTCGGAAT ATAAGGAACC TGGAATTGCC CGCTGTGCCG GTCCCGGAGA AATGGCAGAT
    AAATTGTTAC TCACAACTCC CTCCAAAAAA TTTACATGTC AAGGGCTCCC AGGGCCCTGT
    GGCTTCCTAA TCCCAGGTCC TGTGGATGTT ACTATTCAAG CCAAGTGTAA CCCCTGCTTG
    TCAAATCCAT GTAAAAATGA TGGCACCTGT AACAATGACC CGGTGGATTT TTATCGATGC
    ACCTGCCCAT ATGGTTTCAA GGGCCAGGAC TGTGATGTCC CCATTCATGC CTGTATCAGT
    AATCCATGTA AACATGGAGG AACTTGCCAC TTAAAAGAAG GAGAGAATGA TGGATTCTGG
    TGTACTTGTG CTGATGGGTT TGAAGGAGAA AGCTGTGACA TCAATATTGA TGATTGCGAA
    GATAATGATT GTGAAAATAA TTCTACATGC GTTGATGGAA TTAACAACTA CACGTGTCTT
    TGCCCACCGG AATACACAGC TGCTAATCTG AATGAGGTGG AAAAAGGCGA ACTGTGTGAG
    GAAAAACTGG ACTTCTGTGC ACAAGACCTG AATCCCTGCC AGCATGACTC CAAGTGCATC
    CTGACGCCAA AGGGATTCAA GTGTGACTGC ACTCCGGGAT ACATTGGTGA GCACTGTGAC
    ATCGACTTTG ATGACTGCCA AGATAACAAG TGCAAAAACG GTGCTCATTG CACAGATGCA
    GTGAACGGAT ACACATGTGT CTGTCCTGAA GGCTACAGTG GCTTGTTCTG TGAGTTTTCT
    CCACCCATGG TCCTCCCTCG CACCAGCCCC TGTGATAATT TTGATTGTCA GAATGGAGCC
    CAGTGTATCA TCAGGGTGAA TGAACCAATA TGCCAGTGTT TGCCTGGCTA CTTGGGAGAG
    AAGTGTGAGA AATTGGTCAG TGTGAATTTT GTAAACAAAG AGTCCTATCT TCAGATTCCT
    TCAGCCAAGG TTCGACCTCA GACAAACATC ACACTTCAGA TTGCCACAGA TGAAGACAGC
    GGCATCCTCT TGTACAAGGG TGACAAGGAC CACATTGCTG TGGAACTCTA TCGAGGGCGA
    GTTCGAGCCA GCTATGACAC CGGCTCTCAC CCGGCTTCTG CCATTTACAG TGTGGAGACA
    ATCAATGATG GAAACTTCCA CATTGTAGAG CTACTGACCC TGGATTCGAG TCTTTCCCTC
    TCTGTGGATG GAGGAAGCCC TAAAATCATC ACCAATTTGT CAAAACAATC TACTCTGAAT
    TTCGACTCTC CACTTTACGT AGGAGGTATG CCTGGGAAAA ATAACGTGGC TTCGCTGCGC
    CAGGCCCCTG GGCAGAACGG CACCAGCTTC CATGGCTGTA TCCGGAACCT TTACATTAAC
    AGTGAACTGC AGGACTTCCG GAAAGTGCCT ATGCAAACCG GAATTCTGCC TGGCTGTGAA
    CCATGCCACA AGAAAGTGTG TGCCCATGGC ACATGCCAGC CCAGCAGCCA ATCAGGCTTC
    ACCTGTGAAT GTGAGGAAGG GTGGATGGGG CCCCTCTGTG ACCAGAGAAC CAATGATCCC
    TGTCTCGGAA ACAAATGTGT ACATGGGACC TGCTTGCCCA TCAACGCCTT CTCCTACAGC
    TGCAAGTGCC TGGAGGGCCA CGGCGGGGTC CTCTGTGATG AAGAAGAAGA TCTGTTTAAC
    CCCTGCCAGG TGATCAAGTG CAAGCACGGG AAGTGCAGGC TCTCTGGGCT CGGGCAGCCC
    TATTGTGAAT GCAGCAGTGG ATTCACCGGG GACAGCTGTG ACAGAGAAAT TTCTTGTCGA
    GGGGAACGGA TAAGGGATTA TTACCAAAAG CAGCAGGGTT ACGCTGCCTG TCAAACGACT
    AAGAAAGTAT CTCGCTTGGA GTGCAGAGGC GGGTGTGCTG GGGGGCAGTG CTGTGGACCT
    CTGAGAAGCA AGAGGCGGAA ATACTCTTTC GAATGCACAG ATGGATCTTC ATTTGTGGAC
    GAGGTCGAGA AGGTGGTGAA GTGCGGCTGC ACGAGATGTG CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454759.1
    CDS1650..6317
    Translation

    Target ORF information:

    RefSeq Version XM_017599270.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC
    CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CGATTGCTTG TACCTGTAGC
    AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG TCATCCCTCC AGGAGCATTC
    TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA ACCAGATCTC GGAACTTGCT
    CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG TCCTGTATGG AAATAAAATC
    ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTACT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT
    CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG GCACCTTCTC AGCTCTCAGA
    GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG
    CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGTCCC
    CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAGCAGT ATTTCATTCC AGGAATTCAC CATTTGGCCT GTACAGAAGA TTATCGATCA
    AAATTAAGTG GAGACTGCTT TGCAGACTTG GCTTGTCCTG AAAAATGTCG CTGTGAAGGG
    ACCACAGTAG ACTGCTCCAA TCAAAAACTC AACAAAATCC CAGACCATAT TCCCCAGTAC
    ACAGCAGAGC TGCGTCTCAA TAATAATGAA TTCACAGTGT TAGAAGCCAC GGGAATATTT
    AAGAAACTTC CTCAATTGCG TAAAATCAAC CTTAGCAACA ATAAGATCAC TGATATCGAG
    GAGGGGGCAT TCGAAGGTGC GTCTGGTGTG AATGAGATTC TGCTTACCAG TAACCGTTTG
    GAAAATGTTC AGCATAAGAT GTTCAAAGGA TTGGAGAGCC TCAAAACATT GATGCTGAGA
    AGTAATCGAA TAAGCTGTGT GGGAAACGAC AGTTTCACAG GACTCGGTTC TGTGCGTCTG
    CTCTCTTTAT ATGACAATCA AATTACCACA GTTGCACCAG GAGCATTTGG TACTCTCCAT
    TCATTATCTA CACTAAACCT CTTGGCCAAT CCTTTCAACT GTAACTGTCA CCTGGCATGG
    CTTGGAGAAT GGCTCAGAAG GAAAAGAATT GTAACAGGAA ATCCTCGATG CCAAAAACCC
    TACTTCTTGA AGGAAATACC AATCCAGGAT GTAGCCATTC AGGACTTCAC CTGTGATGAC
    GGAAACGATG ATAATAGCTG CTCTCCACTC TCCCGTTGTC CTTCGGAATG TACTTGCTTG
    GATACAGTAG TACGATGTAG CAACAAGGGC TTGAAGGTCT TACCTAAAGG CATTCCAAGA
    GATGTCACAG AACTGTATCT GGATGGGAAC CAGTTTACAC TGGTCCCGAA GGAACTCTCC
    AACTACAAAC ATTTAACACT TATAGACTTA AGTAACAACA GAATAAGCAC CCTTTCCAAC
    CAAAGCTTCA GCAACATGAC CCAACTTCTC ACCTTAATTC TCAGTTACAA CCGTCTGAGA
    TGTATCCCTC CACGGACCTT TGATGGATTG AAATCTCTTC GTTTACTGTC TCTACATGGA
    AATGACATTT CTGTCGTGCC TGAAGGTGCC TTTGGTGACC TTTCAGCCTT GTCACACTTA
    GCAATTGGAG CCAACCCTCT TTACTGTGAT TGTAACATGC AGTGGTTATC CGACTGGGTG
    AAGTCGGAAT ATAAGGAACC TGGAATTGCC CGCTGTGCCG GTCCCGGAGA AATGGCAGAT
    AAATTGTTAC TCACAACTCC CTCCAAAAAA TTTACATGTC AAGGGCTCCC AGGGCCCTGT
    GGCTTCCTAA TCCCAGGTCC TGTGGATGTT ACTATTCAAG CCAAGTGTAA CCCCTGCTTG
    TCAAATCCAT GTAAAAATGA TGGCACCTGT AACAATGACC CGGTGGATTT TTATCGATGC
    ACCTGCCCAT ATGGTTTCAA GGGCCAGGAC TGTGATGTCC CCATTCATGC CTGTATCAGT
    AATCCATGTA AACATGGAGG AACTTGCCAC TTAAAAGAAG GAGAGAATGA TGGATTCTGG
    TGTACTTGTG CTGATGGGTT TGAAGGAGAA AGCTGTGACA TCAATATTGA TGATTGCGAA
    GATAATGATT GTGAAAATAA TTCTACATGC GTTGATGGAA TTAACAACTA CACGTGTCTT
    TGCCCACCGG AATACACAGC TGCTAATCTG AATGAGGTGG AAAAAGGCGA ACTGTGTGAG
    GAAAAACTGG ACTTCTGTGC ACAAGACCTG AATCCCTGCC AGCATGACTC CAAGTGCATC
    CTGACGCCAA AGGGATTCAA GTGTGACTGC ACTCCGGGAT ACATTGGTGA GCACTGTGAC
    ATCGACTTTG ATGACTGCCA AGATAACAAG TGCAAAAACG GTGCTCATTG CACAGATGCA
    GTGAACGGAT ACACATGTGT CTGTCCTGAA GGCTACAGTG GCTTGTTCTG TGAGTTTTCT
    CCACCCATGG TCCTCCCTCG CACCAGCCCC TGTGATAATT TTGATTGTCA GAATGGAGCC
    CAGTGTATCA TCAGGGTGAA TGAACCAATA TGCCAGTGTT TGCCTGGCTA CTTGGGAGAG
    AAGTGTGAGA AATTGGTCAG TGTGAATTTT GTAAACAAAG AGTCCTATCT TCAGATTCCT
    TCAGCCAAGG TTCGACCTCA GACAAACATC ACACTTCAGA TTGCCACAGA TGAAGACAGC
    GGCATCCTCT TGTACAAGGG TGACAAGGAC CACATTGCTG TGGAACTCTA TCGAGGGCGA
    GTTCGAGCCA GCTATGACAC CGGCTCTCAC CCGGCTTCTG CCATTTACAG TGTGGAGACA
    ATCAATGATG GAAACTTCCA CATTGTAGAG CTACTGACCC TGGATTCGAG TCTTTCCCTC
    TCTGTGGATG GAGGAAGCCC TAAAATCATC ACCAATTTGT CAAAACAATC TACTCTGAAT
    TTCGACTCTC CACTTTACGT AGGAGGTATG CCTGGGAAAA ATAACGTGGC TTCGCTGCGC
    CAGGCCCCTG GGCAGAACGG CACCAGCTTC CATGGCTGTA TCCGGAACCT TTACATTAAC
    AGTGAACTGC AGGACTTCCG GAAAGTGCCT ATGCAAACCG GAATTCTGCC TGGCTGTGAA
    CCATGCCACA AGAAAGTGTG TGCCCATGGC ACATGCCAGC CCAGCAGCCA ATCAGGCTTC
    ACCTGTGAAT GTGAGGAAGG GTGGATGGGG CCCCTCTGTG ACCAGAGAAC CAATGATCCC
    TGTCTCGGAA ACAAATGTGT ACATGGGACC TGCTTGCCCA TCAACGCCTT CTCCTACAGC
    TGCAAGTGCC TGGAGGGCCA CGGCGGGGTC CTCTGTGATG AAGAAGAAGA TCTGTTTAAC
    CCCTGCCAGG TGATCAAGTG CAAGCACGGG AAGTGCAGGC TCTCTGGGCT CGGGCAGCCC
    TATTGTGAAT GCAGCAGTGG ATTCACCGGG GACAGCTGTG ACAGAGAAAT TTCTTGTCGA
    GGGGAACGGA TAAGGGATTA TTACCAAAAG CAGCAGGGTT ACGCTGCCTG TCAAACGACT
    AAGAAAGTAT CTCGCTTGGA GTGCAGAGGC GGGTGTGCTG GGGGGCAGTG CTGTGGACCT
    CTGAGAAGCA AGAGGCGGAA ATACTCTTTC GAATGCACAG ATGGATCTTC ATTTGTGGAC
    GAGGTCGAGA AGGTGGTGAA GTGCGGCTGC ACGAGATGTG CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58346
    Clone ID Related Accession (Same CDS sequence) XM_017599271.1
    Accession Version XM_017599271.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAAAAAT GTCGCTGTGA AGGGACCACA
    GTAGACTGCT CCAATCAAAA ACTCAACAAA ATCCCAGACC ATATTCCCCA GTACACAGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA
    CTTCCTCAAT TGCGTAAAAT CAACCTTAGC AACAATAAGA TCACTGATAT CGAGGAGGGG
    GCATTCGAAG GTGCGTCTGG TGTGAATGAG ATTCTGCTTA CCAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA AGGATTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT
    CGAATAAGCT GTGTGGGAAA CGACAGTTTC ACAGGACTCG GTTCTGTGCG TCTGCTCTCT
    TTATATGACA ATCAAATTAC CACAGTTGCA CCAGGAGCAT TTGGTACTCT CCATTCATTA
    TCTACACTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTTGGA
    GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC
    TTGAAGGAAA TACCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGACGGAAAC
    GATGATAATA GCTGCTCTCC ACTCTCCCGT TGTCCTTCGG AATGTACTTG CTTGGATACA
    GTAGTACGAT GTAGCAACAA GGGCTTGAAG GTCTTACCTA AAGGCATTCC AAGAGATGTC
    ACAGAACTGT ATCTGGATGG GAACCAGTTT ACACTGGTCC CGAAGGAACT CTCCAACTAC
    AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTTTC CAACCAAAGC
    TTCAGCAACA TGACCCAACT TCTCACCTTA ATTCTCAGTT ACAACCGTCT GAGATGTATC
    CCTCCACGGA CCTTTGATGG ATTGAAATCT CTTCGTTTAC TGTCTCTACA TGGAAATGAC
    ATTTCTGTCG TGCCTGAAGG TGCCTTTGGT GACCTTTCAG CCTTGTCACA CTTAGCAATT
    GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG
    GAATATAAGG AACCTGGAAT TGCCCGCTGT GCCGGTCCCG GAGAAATGGC AGATAAATTG
    TTACTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGGC TCCCAGGGCC CTGTGGCTTC
    CTAATCCCAG GTCCTGTGGA TGTTACTATT CAAGCCAAGT GTAACCCCTG CTTGTCAAAT
    CCATGTAAAA ATGATGGCAC CTGTAACAAT GACCCGGTGG ATTTTTATCG ATGCACCTGC
    CCATATGGTT TCAAGGGCCA GGACTGTGAT GTCCCCATTC ATGCCTGTAT CAGTAATCCA
    TGTAAACATG GAGGAACTTG CCACTTAAAA GAAGGAGAGA ATGATGGATT CTGGTGTACT
    TGTGCTGATG GGTTTGAAGG AGAAAGCTGT GACATCAATA TTGATGATTG CGAAGATAAT
    GATTGTGAAA ATAATTCTAC ATGCGTTGAT GGAATTAACA ACTACACGTG TCTTTGCCCA
    CCGGAATACA CAGCTGCTAA TCTGAATGAG GTGGAAAAAG GCGAACTGTG TGAGGAAAAA
    CTGGACTTCT GTGCACAAGA CCTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACG
    CCAAAGGGAT TCAAGTGTGA CTGCACTCCG GGATACATTG GTGAGCACTG TGACATCGAC
    TTTGATGACT GCCAAGATAA CAAGTGCAAA AACGGTGCTC ATTGCACAGA TGCAGTGAAC
    GGATACACAT GTGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC
    ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCCCAGTGT
    ATCATCAGGG TGAATGAACC AATATGCCAG TGTTTGCCTG GCTACTTGGG AGAGAAGTGT
    GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC
    AAGGTTCGAC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC
    CTCTTGTACA AGGGTGACAA GGACCACATT GCTGTGGAAC TCTATCGAGG GCGAGTTCGA
    GCCAGCTATG ACACCGGCTC TCACCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT
    GATGGAAACT TCCACATTGT AGAGCTACTG ACCCTGGATT CGAGTCTTTC CCTCTCTGTG
    GATGGAGGAA GCCCTAAAAT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC
    TCTCCACTTT ACGTAGGAGG TATGCCTGGG AAAAATAACG TGGCTTCGCT GCGCCAGGCC
    CCTGGGCAGA ACGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAA
    CTGCAGGACT TCCGGAAAGT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC
    CACAAGAAAG TGTGTGCCCA TGGCACATGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT
    GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGTCTC
    GGAAACAAAT GTGTACATGG GACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGCAAG
    TGCCTGGAGG GCCACGGCGG GGTCCTCTGT GATGAAGAAG AAGATCTGTT TAACCCCTGC
    CAGGTGATCA AGTGCAAGCA CGGGAAGTGC AGGCTCTCTG GGCTCGGGCA GCCCTATTGT
    GAATGCAGCA GTGGATTCAC CGGGGACAGC TGTGACAGAG AAATTTCTTG TCGAGGGGAA
    CGGATAAGGG ATTATTACCA AAAGCAGCAG GGTTACGCTG CCTGTCAAAC GACTAAGAAA
    GTATCTCGCT TGGAGTGCAG AGGCGGGTGT GCTGGGGGGC AGTGCTGTGG ACCTCTGAGA
    AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGAT CTTCATTTGT GGACGAGGTC
    GAGAAGGTGG TGAAGTGCGG CTGCACGAGA TGTGCCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454760.1
    CDS1650..6311
    Translation

    Target ORF information:

    RefSeq Version XM_017599271.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599271.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAAAAAT GTCGCTGTGA AGGGACCACA
    GTAGACTGCT CCAATCAAAA ACTCAACAAA ATCCCAGACC ATATTCCCCA GTACACAGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA
    CTTCCTCAAT TGCGTAAAAT CAACCTTAGC AACAATAAGA TCACTGATAT CGAGGAGGGG
    GCATTCGAAG GTGCGTCTGG TGTGAATGAG ATTCTGCTTA CCAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA AGGATTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT
    CGAATAAGCT GTGTGGGAAA CGACAGTTTC ACAGGACTCG GTTCTGTGCG TCTGCTCTCT
    TTATATGACA ATCAAATTAC CACAGTTGCA CCAGGAGCAT TTGGTACTCT CCATTCATTA
    TCTACACTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTTGGA
    GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC
    TTGAAGGAAA TACCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGACGGAAAC
    GATGATAATA GCTGCTCTCC ACTCTCCCGT TGTCCTTCGG AATGTACTTG CTTGGATACA
    GTAGTACGAT GTAGCAACAA GGGCTTGAAG GTCTTACCTA AAGGCATTCC AAGAGATGTC
    ACAGAACTGT ATCTGGATGG GAACCAGTTT ACACTGGTCC CGAAGGAACT CTCCAACTAC
    AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTTTC CAACCAAAGC
    TTCAGCAACA TGACCCAACT TCTCACCTTA ATTCTCAGTT ACAACCGTCT GAGATGTATC
    CCTCCACGGA CCTTTGATGG ATTGAAATCT CTTCGTTTAC TGTCTCTACA TGGAAATGAC
    ATTTCTGTCG TGCCTGAAGG TGCCTTTGGT GACCTTTCAG CCTTGTCACA CTTAGCAATT
    GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG
    GAATATAAGG AACCTGGAAT TGCCCGCTGT GCCGGTCCCG GAGAAATGGC AGATAAATTG
    TTACTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGGC TCCCAGGGCC CTGTGGCTTC
    CTAATCCCAG GTCCTGTGGA TGTTACTATT CAAGCCAAGT GTAACCCCTG CTTGTCAAAT
    CCATGTAAAA ATGATGGCAC CTGTAACAAT GACCCGGTGG ATTTTTATCG ATGCACCTGC
    CCATATGGTT TCAAGGGCCA GGACTGTGAT GTCCCCATTC ATGCCTGTAT CAGTAATCCA
    TGTAAACATG GAGGAACTTG CCACTTAAAA GAAGGAGAGA ATGATGGATT CTGGTGTACT
    TGTGCTGATG GGTTTGAAGG AGAAAGCTGT GACATCAATA TTGATGATTG CGAAGATAAT
    GATTGTGAAA ATAATTCTAC ATGCGTTGAT GGAATTAACA ACTACACGTG TCTTTGCCCA
    CCGGAATACA CAGCTGCTAA TCTGAATGAG GTGGAAAAAG GCGAACTGTG TGAGGAAAAA
    CTGGACTTCT GTGCACAAGA CCTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACG
    CCAAAGGGAT TCAAGTGTGA CTGCACTCCG GGATACATTG GTGAGCACTG TGACATCGAC
    TTTGATGACT GCCAAGATAA CAAGTGCAAA AACGGTGCTC ATTGCACAGA TGCAGTGAAC
    GGATACACAT GTGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC
    ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCCCAGTGT
    ATCATCAGGG TGAATGAACC AATATGCCAG TGTTTGCCTG GCTACTTGGG AGAGAAGTGT
    GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC
    AAGGTTCGAC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC
    CTCTTGTACA AGGGTGACAA GGACCACATT GCTGTGGAAC TCTATCGAGG GCGAGTTCGA
    GCCAGCTATG ACACCGGCTC TCACCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT
    GATGGAAACT TCCACATTGT AGAGCTACTG ACCCTGGATT CGAGTCTTTC CCTCTCTGTG
    GATGGAGGAA GCCCTAAAAT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC
    TCTCCACTTT ACGTAGGAGG TATGCCTGGG AAAAATAACG TGGCTTCGCT GCGCCAGGCC
    CCTGGGCAGA ACGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAA
    CTGCAGGACT TCCGGAAAGT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC
    CACAAGAAAG TGTGTGCCCA TGGCACATGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT
    GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGTCTC
    GGAAACAAAT GTGTACATGG GACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGCAAG
    TGCCTGGAGG GCCACGGCGG GGTCCTCTGT GATGAAGAAG AAGATCTGTT TAACCCCTGC
    CAGGTGATCA AGTGCAAGCA CGGGAAGTGC AGGCTCTCTG GGCTCGGGCA GCCCTATTGT
    GAATGCAGCA GTGGATTCAC CGGGGACAGC TGTGACAGAG AAATTTCTTG TCGAGGGGAA
    CGGATAAGGG ATTATTACCA AAAGCAGCAG GGTTACGCTG CCTGTCAAAC GACTAAGAAA
    GTATCTCGCT TGGAGTGCAG AGGCGGGTGT GCTGGGGGGC AGTGCTGTGG ACCTCTGAGA
    AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGAT CTTCATTTGT GGACGAGGTC
    GAGAAGGTGG TGAAGTGCGG CTGCACGAGA TGTGCCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58347
    Clone ID Related Accession (Same CDS sequence) XM_017599272.1
    Accession Version XM_017599272.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4653bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGAATTC ACCATTTGGC CTGTACAGAA
    GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAAAAATGT
    CGCTGTGAAG GGACCACAGT AGACTGCTCC AATCAAAAAC TCAACAAAAT CCCAGACCAT
    ATTCCCCAGT ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC
    ACGGGAATAT TTAAGAAACT TCCTCAATTG CGTAAAATCA ACCTTAGCAA CAATAAGATC
    ACTGATATCG AGGAGGGGGC ATTCGAAGGT GCGTCTGGTG TGAATGAGAT TCTGCTTACC
    AGTAACCGTT TGGAAAATGT TCAGCATAAG ATGTTCAAAG GATTGGAGAG CCTCAAAACA
    TTGATGCTGA GAAGTAATCG AATAAGCTGT GTGGGAAACG ACAGTTTCAC AGGACTCGGT
    TCTGTGCGTC TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTTGCACC AGGAGCATTT
    GGTACTCTCC ATTCATTATC TACACTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT
    CACCTGGCAT GGCTTGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA
    TGCCAAAAAC CCTACTTCTT GAAGGAAATA CCAATCCAGG ATGTAGCCAT TCAGGACTTC
    ACCTGTGATG ACGGAAACGA TGATAATAGC TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA
    TGTACTTGCT TGGATACAGT AGTACGATGT AGCAACAAGG GCTTGAAGGT CTTACCTAAA
    GGCATTCCAA GAGATGTCAC AGAACTGTAT CTGGATGGGA ACCAGTTTAC ACTGGTCCCG
    AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC
    ACCCTTTCCA ACCAAAGCTT CAGCAACATG ACCCAACTTC TCACCTTAAT TCTCAGTTAC
    AACCGTCTGA GATGTATCCC TCCACGGACC TTTGATGGAT TGAAATCTCT TCGTTTACTG
    TCTCTACATG GAAATGACAT TTCTGTCGTG CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC
    TTGTCACACT TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA
    TCCGACTGGG TGAAGTCGGA ATATAAGGAA CCTGGAATTG CCCGCTGTGC CGGTCCCGGA
    GAAATGGCAG ATAAATTGTT ACTCACAACT CCCTCCAAAA AATTTACATG TCAAGGGCTC
    CCAGGGCCCT GTGGCTTCCT AATCCCAGGT CCTGTGGATG TTACTATTCA AGCCAAGTGT
    AACCCCTGCT TGTCAAATCC ATGTAAAAAT GATGGCACCT GTAACAATGA CCCGGTGGAT
    TTTTATCGAT GCACCTGCCC ATATGGTTTC AAGGGCCAGG ACTGTGATGT CCCCATTCAT
    GCCTGTATCA GTAATCCATG TAAACATGGA GGAACTTGCC ACTTAAAAGA AGGAGAGAAT
    GATGGATTCT GGTGTACTTG TGCTGATGGG TTTGAAGGAG AAAGCTGTGA CATCAATATT
    GATGATTGCG AAGATAATGA TTGTGAAAAT AATTCTACAT GCGTTGATGG AATTAACAAC
    TACACGTGTC TTTGCCCACC GGAATACACA GGCGAACTGT GTGAGGAAAA ACTGGACTTC
    TGTGCACAAG ACCTGAATCC CTGCCAGCAT GACTCCAAGT GCATCCTGAC GCCAAAGGGA
    TTCAAGTGTG ACTGCACTCC GGGATACATT GGTGAGCACT GTGACATCGA CTTTGATGAC
    TGCCAAGATA ACAAGTGCAA AAACGGTGCT CATTGCACAG ATGCAGTGAA CGGATACACA
    TGTGTCTGTC CTGAAGGCTA CAGTGGCTTG TTCTGTGAGT TTTCTCCACC CATGGTCCTC
    CCTCGCACCA GCCCCTGTGA TAATTTTGAT TGTCAGAATG GAGCCCAGTG TATCATCAGG
    GTGAATGAAC CAATATGCCA GTGTTTGCCT GGCTACTTGG GAGAGAAGTG TGAGAAATTG
    GTCAGTGTGA ATTTTGTAAA CAAAGAGTCC TATCTTCAGA TTCCTTCAGC CAAGGTTCGA
    CCTCAGACAA ACATCACACT TCAGATTGCC ACAGATGAAG ACAGCGGCAT CCTCTTGTAC
    AAGGGTGACA AGGACCACAT TGCTGTGGAA CTCTATCGAG GGCGAGTTCG AGCCAGCTAT
    GACACCGGCT CTCACCCGGC TTCTGCCATT TACAGTGTGG AGACAATCAA TGATGGAAAC
    TTCCACATTG TAGAGCTACT GACCCTGGAT TCGAGTCTTT CCCTCTCTGT GGATGGAGGA
    AGCCCTAAAA TCATCACCAA TTTGTCAAAA CAATCTACTC TGAATTTCGA CTCTCCACTT
    TACGTAGGAG GTATGCCTGG GAAAAATAAC GTGGCTTCGC TGCGCCAGGC CCCTGGGCAG
    AACGGCACCA GCTTCCATGG CTGTATCCGG AACCTTTACA TTAACAGTGA ACTGCAGGAC
    TTCCGGAAAG TGCCTATGCA AACCGGAATT CTGCCTGGCT GTGAACCATG CCACAAGAAA
    GTGTGTGCCC ATGGCACATG CCAGCCCAGC AGCCAATCAG GCTTCACCTG TGAATGTGAG
    GAAGGGTGGA TGGGGCCCCT CTGTGACCAG AGAACCAATG ATCCCTGTCT CGGAAACAAA
    TGTGTACATG GGACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGCAA GTGCCTGGAG
    GGCCACGGCG GGGTCCTCTG TGATGAAGAA GAAGATCTGT TTAACCCCTG CCAGGTGATC
    AAGTGCAAGC ACGGGAAGTG CAGGCTCTCT GGGCTCGGGC AGCCCTATTG TGAATGCAGC
    AGTGGATTCA CCGGGGACAG CTGTGACAGA GAAATTTCTT GTCGAGGGGA ACGGATAAGG
    GATTATTACC AAAAGCAGCA GGGTTACGCT GCCTGTCAAA CGACTAAGAA AGTATCTCGC
    TTGGAGTGCA GAGGCGGGTG TGCTGGGGGG CAGTGCTGTG GACCTCTGAG AAGCAAGAGG
    CGGAAATACT CTTTCGAATG CACAGATGGA TCTTCATTTG TGGACGAGGT CGAGAAGGTG
    GTGAAGTGCG GCTGCACGAG ATGTGCCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454761.1
    CDS1650..6302
    Translation

    Target ORF information:

    RefSeq Version XM_017599272.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599272.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGAATTC ACCATTTGGC CTGTACAGAA
    GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAAAAATGT
    CGCTGTGAAG GGACCACAGT AGACTGCTCC AATCAAAAAC TCAACAAAAT CCCAGACCAT
    ATTCCCCAGT ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC
    ACGGGAATAT TTAAGAAACT TCCTCAATTG CGTAAAATCA ACCTTAGCAA CAATAAGATC
    ACTGATATCG AGGAGGGGGC ATTCGAAGGT GCGTCTGGTG TGAATGAGAT TCTGCTTACC
    AGTAACCGTT TGGAAAATGT TCAGCATAAG ATGTTCAAAG GATTGGAGAG CCTCAAAACA
    TTGATGCTGA GAAGTAATCG AATAAGCTGT GTGGGAAACG ACAGTTTCAC AGGACTCGGT
    TCTGTGCGTC TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTTGCACC AGGAGCATTT
    GGTACTCTCC ATTCATTATC TACACTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT
    CACCTGGCAT GGCTTGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA
    TGCCAAAAAC CCTACTTCTT GAAGGAAATA CCAATCCAGG ATGTAGCCAT TCAGGACTTC
    ACCTGTGATG ACGGAAACGA TGATAATAGC TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA
    TGTACTTGCT TGGATACAGT AGTACGATGT AGCAACAAGG GCTTGAAGGT CTTACCTAAA
    GGCATTCCAA GAGATGTCAC AGAACTGTAT CTGGATGGGA ACCAGTTTAC ACTGGTCCCG
    AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC
    ACCCTTTCCA ACCAAAGCTT CAGCAACATG ACCCAACTTC TCACCTTAAT TCTCAGTTAC
    AACCGTCTGA GATGTATCCC TCCACGGACC TTTGATGGAT TGAAATCTCT TCGTTTACTG
    TCTCTACATG GAAATGACAT TTCTGTCGTG CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC
    TTGTCACACT TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA
    TCCGACTGGG TGAAGTCGGA ATATAAGGAA CCTGGAATTG CCCGCTGTGC CGGTCCCGGA
    GAAATGGCAG ATAAATTGTT ACTCACAACT CCCTCCAAAA AATTTACATG TCAAGGGCTC
    CCAGGGCCCT GTGGCTTCCT AATCCCAGGT CCTGTGGATG TTACTATTCA AGCCAAGTGT
    AACCCCTGCT TGTCAAATCC ATGTAAAAAT GATGGCACCT GTAACAATGA CCCGGTGGAT
    TTTTATCGAT GCACCTGCCC ATATGGTTTC AAGGGCCAGG ACTGTGATGT CCCCATTCAT
    GCCTGTATCA GTAATCCATG TAAACATGGA GGAACTTGCC ACTTAAAAGA AGGAGAGAAT
    GATGGATTCT GGTGTACTTG TGCTGATGGG TTTGAAGGAG AAAGCTGTGA CATCAATATT
    GATGATTGCG AAGATAATGA TTGTGAAAAT AATTCTACAT GCGTTGATGG AATTAACAAC
    TACACGTGTC TTTGCCCACC GGAATACACA GGCGAACTGT GTGAGGAAAA ACTGGACTTC
    TGTGCACAAG ACCTGAATCC CTGCCAGCAT GACTCCAAGT GCATCCTGAC GCCAAAGGGA
    TTCAAGTGTG ACTGCACTCC GGGATACATT GGTGAGCACT GTGACATCGA CTTTGATGAC
    TGCCAAGATA ACAAGTGCAA AAACGGTGCT CATTGCACAG ATGCAGTGAA CGGATACACA
    TGTGTCTGTC CTGAAGGCTA CAGTGGCTTG TTCTGTGAGT TTTCTCCACC CATGGTCCTC
    CCTCGCACCA GCCCCTGTGA TAATTTTGAT TGTCAGAATG GAGCCCAGTG TATCATCAGG
    GTGAATGAAC CAATATGCCA GTGTTTGCCT GGCTACTTGG GAGAGAAGTG TGAGAAATTG
    GTCAGTGTGA ATTTTGTAAA CAAAGAGTCC TATCTTCAGA TTCCTTCAGC CAAGGTTCGA
    CCTCAGACAA ACATCACACT TCAGATTGCC ACAGATGAAG ACAGCGGCAT CCTCTTGTAC
    AAGGGTGACA AGGACCACAT TGCTGTGGAA CTCTATCGAG GGCGAGTTCG AGCCAGCTAT
    GACACCGGCT CTCACCCGGC TTCTGCCATT TACAGTGTGG AGACAATCAA TGATGGAAAC
    TTCCACATTG TAGAGCTACT GACCCTGGAT TCGAGTCTTT CCCTCTCTGT GGATGGAGGA
    AGCCCTAAAA TCATCACCAA TTTGTCAAAA CAATCTACTC TGAATTTCGA CTCTCCACTT
    TACGTAGGAG GTATGCCTGG GAAAAATAAC GTGGCTTCGC TGCGCCAGGC CCCTGGGCAG
    AACGGCACCA GCTTCCATGG CTGTATCCGG AACCTTTACA TTAACAGTGA ACTGCAGGAC
    TTCCGGAAAG TGCCTATGCA AACCGGAATT CTGCCTGGCT GTGAACCATG CCACAAGAAA
    GTGTGTGCCC ATGGCACATG CCAGCCCAGC AGCCAATCAG GCTTCACCTG TGAATGTGAG
    GAAGGGTGGA TGGGGCCCCT CTGTGACCAG AGAACCAATG ATCCCTGTCT CGGAAACAAA
    TGTGTACATG GGACCTGCTT GCCCATCAAC GCCTTCTCCT ACAGCTGCAA GTGCCTGGAG
    GGCCACGGCG GGGTCCTCTG TGATGAAGAA GAAGATCTGT TTAACCCCTG CCAGGTGATC
    AAGTGCAAGC ACGGGAAGTG CAGGCTCTCT GGGCTCGGGC AGCCCTATTG TGAATGCAGC
    AGTGGATTCA CCGGGGACAG CTGTGACAGA GAAATTTCTT GTCGAGGGGA ACGGATAAGG
    GATTATTACC AAAAGCAGCA GGGTTACGCT GCCTGTCAAA CGACTAAGAA AGTATCTCGC
    TTGGAGTGCA GAGGCGGGTG TGCTGGGGGG CAGTGCTGTG GACCTCTGAG AAGCAAGAGG
    CGGAAATACT CTTTCGAATG CACAGATGGA TCTTCATTTG TGGACGAGGT CGAGAAGGTG
    GTGAAGTGCG GCTGCACGAG ATGTGCCTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58348
    Clone ID Related Accession (Same CDS sequence) XM_017599273.1
    Accession Version XM_017599273.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGAATTC ACCATTTGGC CTGTACAGAA
    GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAAAAATGT
    CGCTGTGAAG GGACCACAGT AGACTGCTCC AATCAAAAAC TCAACAAAAT CCCAGACCAT
    ATTCCCCAGT ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC
    ACGGGAATAT TTAAGAAACT TCCTCAATTG CGTAAAATCA ACCTTAGCAA CAATAAGATC
    ACTGATATCG AGGAGGGGGC ATTCGAAGGT GCGTCTGGTG TGAATGAGAT TCTGCTTACC
    AGTAACCGTT TGGAAAATGT TCAGCATAAG ATGTTCAAAG GATTGGAGAG CCTCAAAACA
    TTGATGCTGA GAAGTAATCG AATAAGCTGT GTGGGAAACG ACAGTTTCAC AGGACTCGGT
    TCTGTGCGTC TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTTGCACC AGGAGCATTT
    GGTACTCTCC ATTCATTATC TACACTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT
    CACCTGGCAT GGCTTGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA
    TGCCAAAAAC CCTACTTCTT GAAGGAAATA CCAATCCAGG ATGTAGCCAT TCAGGACTTC
    ACCTGTGATG ACGGAAACGA TGATAATAGC TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA
    TGTACTTGCT TGGATACAGT AGTACGATGT AGCAACAAGG GCTTGAAGGT CTTACCTAAA
    GGCATTCCAA GAGATGTCAC AGAACTGTAT CTGGATGGGA ACCAGTTTAC ACTGGTCCCG
    AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC
    ACCCTTTCCA ACCAAAGCTT CAGCAACATG ACCCAACTTC TCACCTTAAT TCTCAGTTAC
    AACCGTCTGA GATGTATCCC TCCACGGACC TTTGATGGAT TGAAATCTCT TCGTTTACTG
    TCTCTACATG GAAATGACAT TTCTGTCGTG CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC
    TTGTCACACT TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA
    TCCGACTGGG TGAAGTCGGA ATATAAGGAA CCTGGAATTG CCCGCTGTGC CGGTCCCGGA
    GAAATGGCAG ATAAATTGTT ACTCACAACT CCCTCCAAAA AATTTACATG TCAAGGTCCT
    GTGGATGTTA CTATTCAAGC CAAGTGTAAC CCCTGCTTGT CAAATCCATG TAAAAATGAT
    GGCACCTGTA ACAATGACCC GGTGGATTTT TATCGATGCA CCTGCCCATA TGGTTTCAAG
    GGCCAGGACT GTGATGTCCC CATTCATGCC TGTATCAGTA ATCCATGTAA ACATGGAGGA
    ACTTGCCACT TAAAAGAAGG AGAGAATGAT GGATTCTGGT GTACTTGTGC TGATGGGTTT
    GAAGGAGAAA GCTGTGACAT CAATATTGAT GATTGCGAAG ATAATGATTG TGAAAATAAT
    TCTACATGCG TTGATGGAAT TAACAACTAC ACGTGTCTTT GCCCACCGGA ATACACAGCT
    GCTAATCTGA ATGAGGTGGA AAAAGGCGAA CTGTGTGAGG AAAAACTGGA CTTCTGTGCA
    CAAGACCTGA ATCCCTGCCA GCATGACTCC AAGTGCATCC TGACGCCAAA GGGATTCAAG
    TGTGACTGCA CTCCGGGATA CATTGGTGAG CACTGTGACA TCGACTTTGA TGACTGCCAA
    GATAACAAGT GCAAAAACGG TGCTCATTGC ACAGATGCAG TGAACGGATA CACATGTGTC
    TGTCCTGAAG GCTACAGTGG CTTGTTCTGT GAGTTTTCTC CACCCATGGT CCTCCCTCGC
    ACCAGCCCCT GTGATAATTT TGATTGTCAG AATGGAGCCC AGTGTATCAT CAGGGTGAAT
    GAACCAATAT GCCAGTGTTT GCCTGGCTAC TTGGGAGAGA AGTGTGAGAA ATTGGTCAGT
    GTGAATTTTG TAAACAAAGA GTCCTATCTT CAGATTCCTT CAGCCAAGGT TCGACCTCAG
    ACAAACATCA CACTTCAGAT TGCCACAGAT GAAGACAGCG GCATCCTCTT GTACAAGGGT
    GACAAGGACC ACATTGCTGT GGAACTCTAT CGAGGGCGAG TTCGAGCCAG CTATGACACC
    GGCTCTCACC CGGCTTCTGC CATTTACAGT GTGGAGACAA TCAATGATGG AAACTTCCAC
    ATTGTAGAGC TACTGACCCT GGATTCGAGT CTTTCCCTCT CTGTGGATGG AGGAAGCCCT
    AAAATCATCA CCAATTTGTC AAAACAATCT ACTCTGAATT TCGACTCTCC ACTTTACGTA
    GGAGGTATGC CTGGGAAAAA TAACGTGGCT TCGCTGCGCC AGGCCCCTGG GCAGAACGGC
    ACCAGCTTCC ATGGCTGTAT CCGGAACCTT TACATTAACA GTGAACTGCA GGACTTCCGG
    AAAGTGCCTA TGCAAACCGG AATTCTGCCT GGCTGTGAAC CATGCCACAA GAAAGTGTGT
    GCCCATGGCA CATGCCAGCC CAGCAGCCAA TCAGGCTTCA CCTGTGAATG TGAGGAAGGG
    TGGATGGGGC CCCTCTGTGA CCAGAGAACC AATGATCCCT GTCTCGGAAA CAAATGTGTA
    CATGGGACCT GCTTGCCCAT CAACGCCTTC TCCTACAGCT GCAAGTGCCT GGAGGGCCAC
    GGCGGGGTCC TCTGTGATGA AGAAGAAGAT CTGTTTAACC CCTGCCAGGT GATCAAGTGC
    AAGCACGGGA AGTGCAGGCT CTCTGGGCTC GGGCAGCCCT ATTGTGAATG CAGCAGTGGA
    TTCACCGGGG ACAGCTGTGA CAGAGAAATT TCTTGTCGAG GGGAACGGAT AAGGGATTAT
    TACCAAAAGC AGCAGGGTTA CGCTGCCTGT CAAACGACTA AGAAAGTATC TCGCTTGGAG
    TGCAGAGGCG GGTGTGCTGG GGGGCAGTGC TGTGGACCTC TGAGAAGCAA GAGGCGGAAA
    TACTCTTTCG AATGCACAGA TGGATCTTCA TTTGTGGACG AGGTCGAGAA GGTGGTGAAG
    TGCGGCTGCA CGAGATGTGC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454762.1
    CDS1650..6296
    Translation

    Target ORF information:

    RefSeq Version XM_017599273.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599273.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGAATTC ACCATTTGGC CTGTACAGAA
    GATTATCGAT CAAAATTAAG TGGAGACTGC TTTGCAGACT TGGCTTGTCC TGAAAAATGT
    CGCTGTGAAG GGACCACAGT AGACTGCTCC AATCAAAAAC TCAACAAAAT CCCAGACCAT
    ATTCCCCAGT ACACAGCAGA GCTGCGTCTC AATAATAATG AATTCACAGT GTTAGAAGCC
    ACGGGAATAT TTAAGAAACT TCCTCAATTG CGTAAAATCA ACCTTAGCAA CAATAAGATC
    ACTGATATCG AGGAGGGGGC ATTCGAAGGT GCGTCTGGTG TGAATGAGAT TCTGCTTACC
    AGTAACCGTT TGGAAAATGT TCAGCATAAG ATGTTCAAAG GATTGGAGAG CCTCAAAACA
    TTGATGCTGA GAAGTAATCG AATAAGCTGT GTGGGAAACG ACAGTTTCAC AGGACTCGGT
    TCTGTGCGTC TGCTCTCTTT ATATGACAAT CAAATTACCA CAGTTGCACC AGGAGCATTT
    GGTACTCTCC ATTCATTATC TACACTAAAC CTCTTGGCCA ATCCTTTCAA CTGTAACTGT
    CACCTGGCAT GGCTTGGAGA ATGGCTCAGA AGGAAAAGAA TTGTAACAGG AAATCCTCGA
    TGCCAAAAAC CCTACTTCTT GAAGGAAATA CCAATCCAGG ATGTAGCCAT TCAGGACTTC
    ACCTGTGATG ACGGAAACGA TGATAATAGC TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA
    TGTACTTGCT TGGATACAGT AGTACGATGT AGCAACAAGG GCTTGAAGGT CTTACCTAAA
    GGCATTCCAA GAGATGTCAC AGAACTGTAT CTGGATGGGA ACCAGTTTAC ACTGGTCCCG
    AAGGAACTCT CCAACTACAA ACATTTAACA CTTATAGACT TAAGTAACAA CAGAATAAGC
    ACCCTTTCCA ACCAAAGCTT CAGCAACATG ACCCAACTTC TCACCTTAAT TCTCAGTTAC
    AACCGTCTGA GATGTATCCC TCCACGGACC TTTGATGGAT TGAAATCTCT TCGTTTACTG
    TCTCTACATG GAAATGACAT TTCTGTCGTG CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC
    TTGTCACACT TAGCAATTGG AGCCAACCCT CTTTACTGTG ATTGTAACAT GCAGTGGTTA
    TCCGACTGGG TGAAGTCGGA ATATAAGGAA CCTGGAATTG CCCGCTGTGC CGGTCCCGGA
    GAAATGGCAG ATAAATTGTT ACTCACAACT CCCTCCAAAA AATTTACATG TCAAGGTCCT
    GTGGATGTTA CTATTCAAGC CAAGTGTAAC CCCTGCTTGT CAAATCCATG TAAAAATGAT
    GGCACCTGTA ACAATGACCC GGTGGATTTT TATCGATGCA CCTGCCCATA TGGTTTCAAG
    GGCCAGGACT GTGATGTCCC CATTCATGCC TGTATCAGTA ATCCATGTAA ACATGGAGGA
    ACTTGCCACT TAAAAGAAGG AGAGAATGAT GGATTCTGGT GTACTTGTGC TGATGGGTTT
    GAAGGAGAAA GCTGTGACAT CAATATTGAT GATTGCGAAG ATAATGATTG TGAAAATAAT
    TCTACATGCG TTGATGGAAT TAACAACTAC ACGTGTCTTT GCCCACCGGA ATACACAGCT
    GCTAATCTGA ATGAGGTGGA AAAAGGCGAA CTGTGTGAGG AAAAACTGGA CTTCTGTGCA
    CAAGACCTGA ATCCCTGCCA GCATGACTCC AAGTGCATCC TGACGCCAAA GGGATTCAAG
    TGTGACTGCA CTCCGGGATA CATTGGTGAG CACTGTGACA TCGACTTTGA TGACTGCCAA
    GATAACAAGT GCAAAAACGG TGCTCATTGC ACAGATGCAG TGAACGGATA CACATGTGTC
    TGTCCTGAAG GCTACAGTGG CTTGTTCTGT GAGTTTTCTC CACCCATGGT CCTCCCTCGC
    ACCAGCCCCT GTGATAATTT TGATTGTCAG AATGGAGCCC AGTGTATCAT CAGGGTGAAT
    GAACCAATAT GCCAGTGTTT GCCTGGCTAC TTGGGAGAGA AGTGTGAGAA ATTGGTCAGT
    GTGAATTTTG TAAACAAAGA GTCCTATCTT CAGATTCCTT CAGCCAAGGT TCGACCTCAG
    ACAAACATCA CACTTCAGAT TGCCACAGAT GAAGACAGCG GCATCCTCTT GTACAAGGGT
    GACAAGGACC ACATTGCTGT GGAACTCTAT CGAGGGCGAG TTCGAGCCAG CTATGACACC
    GGCTCTCACC CGGCTTCTGC CATTTACAGT GTGGAGACAA TCAATGATGG AAACTTCCAC
    ATTGTAGAGC TACTGACCCT GGATTCGAGT CTTTCCCTCT CTGTGGATGG AGGAAGCCCT
    AAAATCATCA CCAATTTGTC AAAACAATCT ACTCTGAATT TCGACTCTCC ACTTTACGTA
    GGAGGTATGC CTGGGAAAAA TAACGTGGCT TCGCTGCGCC AGGCCCCTGG GCAGAACGGC
    ACCAGCTTCC ATGGCTGTAT CCGGAACCTT TACATTAACA GTGAACTGCA GGACTTCCGG
    AAAGTGCCTA TGCAAACCGG AATTCTGCCT GGCTGTGAAC CATGCCACAA GAAAGTGTGT
    GCCCATGGCA CATGCCAGCC CAGCAGCCAA TCAGGCTTCA CCTGTGAATG TGAGGAAGGG
    TGGATGGGGC CCCTCTGTGA CCAGAGAACC AATGATCCCT GTCTCGGAAA CAAATGTGTA
    CATGGGACCT GCTTGCCCAT CAACGCCTTC TCCTACAGCT GCAAGTGCCT GGAGGGCCAC
    GGCGGGGTCC TCTGTGATGA AGAAGAAGAT CTGTTTAACC CCTGCCAGGT GATCAAGTGC
    AAGCACGGGA AGTGCAGGCT CTCTGGGCTC GGGCAGCCCT ATTGTGAATG CAGCAGTGGA
    TTCACCGGGG ACAGCTGTGA CAGAGAAATT TCTTGTCGAG GGGAACGGAT AAGGGATTAT
    TACCAAAAGC AGCAGGGTTA CGCTGCCTGT CAAACGACTA AGAAAGTATC TCGCTTGGAG
    TGCAGAGGCG GGTGTGCTGG GGGGCAGTGC TGTGGACCTC TGAGAAGCAA GAGGCGGAAA
    TACTCTTTCG AATGCACAGA TGGATCTTCA TTTGTGGACG AGGTCGAGAA GGTGGTGAAG
    TGCGGCTGCA CGAGATGTGC CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58349
    Clone ID Related Accession (Same CDS sequence) XM_017599274.1
    Accession Version XM_017599274.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG
    GCTTGTCCTG AAAAATGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAAACTC
    AACAAAATCC CAGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAATTGCG TAAAATCAAC
    CTTAGCAACA ATAAGATCAC TGATATCGAG GAGGGGGCAT TCGAAGGTGC GTCTGGTGTG
    AATGAGATTC TGCTTACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA
    TTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT GGGAAACGAC
    AGTTTCACAG GACTCGGTTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA
    GTTGCACCAG GAGCATTTGG TACTCTCCAT TCATTATCTA CACTAAACCT CTTGGCCAAT
    CCTTTCAACT GTAACTGTCA CCTGGCATGG CTTGGAGAAT GGCTCAGAAG GAAAAGAATT
    GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCTTGA AGGAAATACC AATCCAGGAT
    GTAGCCATTC AGGACTTCAC CTGTGATGAC GGAAACGATG ATAATAGCTG CTCTCCACTC
    TCCCGTTGTC CTTCGGAATG TACTTGCTTG GATACAGTAG TACGATGTAG CAACAAGGGC
    TTGAAGGTCT TACCTAAAGG CATTCCAAGA GATGTCACAG AACTGTATCT GGATGGGAAC
    CAGTTTACAC TGGTCCCGAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA
    AGTAACAACA GAATAAGCAC CCTTTCCAAC CAAAGCTTCA GCAACATGAC CCAACTTCTC
    ACCTTAATTC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGGACCTT TGATGGATTG
    AAATCTCTTC GTTTACTGTC TCTACATGGA AATGACATTT CTGTCGTGCC TGAAGGTGCC
    TTTGGTGACC TTTCAGCCTT GTCACACTTA GCAATTGGAG CCAACCCTCT TTACTGTGAT
    TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCC
    CGCTGTGCCG GTCCCGGAGA AATGGCAGAT AAATTGTTAC TCACAACTCC CTCCAAAAAA
    TTTACATGTC AAGGGCTCCC AGGGCCCTGT GGCTTCCTAA TCCCAGGTCC TGTGGATGTT
    ACTATTCAAG CCAAGTGTAA CCCCTGCTTG TCAAATCCAT GTAAAAATGA TGGCACCTGT
    AACAATGACC CGGTGGATTT TTATCGATGC ACCTGCCCAT ATGGTTTCAA GGGCCAGGAC
    TGTGATGTCC CCATTCATGC CTGTATCAGT AATCCATGTA AACATGGAGG AACTTGCCAC
    TTAAAAGAAG GAGAGAATGA TGGATTCTGG TGTACTTGTG CTGATGGGTT TGAAGGAGAA
    AGCTGTGACA TCAATATTGA TGATTGCGAA GATAATGATT GTGAAAATAA TTCTACATGC
    GTTGATGGAA TTAACAACTA CACGTGTCTT TGCCCACCGG AATACACAGC TGCTAATCTG
    AATGAGGTGG AAAAAGGCGA ACTGTGTGAG GAAAAACTGG ACTTCTGTGC ACAAGACCTG
    AATCCCTGCC AGCATGACTC CAAGTGCATC CTGACGCCAA AGGGATTCAA GTGTGACTGC
    ACTCCGGGAT ACATTGGTGA GCACTGTGAC ATCGACTTTG ATGACTGCCA AGATAACAAG
    TGCAAAAACG GTGCTCATTG CACAGATGCA GTGAACGGAT ACACATGTGT CTGTCCTGAA
    GGCTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG CACCAGCCCC
    TGTGATAATT TTGATTGTCA GAATGGAGCC CAGTGTATCA TCAGGGTGAA TGAACCAATA
    TGCCAGTGTT TGCCTGGCTA CTTGGGAGAG AAGTGTGAGA AATTGGTCAG TGTGAATTTT
    GTAAACAAAG AGTCCTATCT TCAGATTCCT TCAGCCAAGG TTCGACCTCA GACAAACATC
    ACACTTCAGA TTGCCACAGA TGAAGACAGC GGCATCCTCT TGTACAAGGG TGACAAGGAC
    CACATTGCTG TGGAACTCTA TCGAGGGCGA GTTCGAGCCA GCTATGACAC CGGCTCTCAC
    CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTAGAG
    CTACTGACCC TGGATTCGAG TCTTTCCCTC TCTGTGGATG GAGGAAGCCC TAAAATCATC
    ACCAATTTGT CAAAACAATC TACTCTGAAT TTCGACTCTC CACTTTACGT AGGAGGTATG
    CCTGGGAAAA ATAACGTGGC TTCGCTGCGC CAGGCCCCTG GGCAGAACGG CACCAGCTTC
    CATGGCTGTA TCCGGAACCT TTACATTAAC AGTGAACTGC AGGACTTCCG GAAAGTGCCT
    ATGCAAACCG GAATTCTGCC TGGCTGTGAA CCATGCCACA AGAAAGTGTG TGCCCATGGC
    ACATGCCAGC CCAGCAGCCA ATCAGGCTTC ACCTGTGAAT GTGAGGAAGG GTGGATGGGG
    CCCCTCTGTG ACCAGAGAAC CAATGATCCC TGTCTCGGAA ACAAATGTGT ACATGGGACC
    TGCTTGCCCA TCAACGCCTT CTCCTACAGC TGCAAGTGCC TGGAGGGCCA CGGCGGGGTC
    CTCTGTGATG AAGAAGAAGA TCTGTTTAAC CCCTGCCAGG TGATCAAGTG CAAGCACGGG
    AAGTGCAGGC TCTCTGGGCT CGGGCAGCCC TATTGTGAAT GCAGCAGTGG ATTCACCGGG
    GACAGCTGTG ACAGAGAAAT TTCTTGTCGA GGGGAACGGA TAAGGGATTA TTACCAAAAG
    CAGCAGGGTT ACGCTGCCTG TCAAACGACT AAGAAAGTAT CTCGCTTGGA GTGCAGAGGC
    GGGTGTGCTG GGGGGCAGTG CTGTGGACCT CTGAGAAGCA AGAGGCGGAA ATACTCTTTC
    GAATGCACAG ATGGATCTTC ATTTGTGGAC GAGGTCGAGA AGGTGGTGAA GTGCGGCTGC
    ACGAGATGTG CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454763.1
    CDS1650..6287
    Translation

    Target ORF information:

    RefSeq Version XM_017599274.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599274.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG
    GCTTGTCCTG AAAAATGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAAACTC
    AACAAAATCC CAGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAATTGCG TAAAATCAAC
    CTTAGCAACA ATAAGATCAC TGATATCGAG GAGGGGGCAT TCGAAGGTGC GTCTGGTGTG
    AATGAGATTC TGCTTACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA
    TTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT GGGAAACGAC
    AGTTTCACAG GACTCGGTTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA
    GTTGCACCAG GAGCATTTGG TACTCTCCAT TCATTATCTA CACTAAACCT CTTGGCCAAT
    CCTTTCAACT GTAACTGTCA CCTGGCATGG CTTGGAGAAT GGCTCAGAAG GAAAAGAATT
    GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCTTGA AGGAAATACC AATCCAGGAT
    GTAGCCATTC AGGACTTCAC CTGTGATGAC GGAAACGATG ATAATAGCTG CTCTCCACTC
    TCCCGTTGTC CTTCGGAATG TACTTGCTTG GATACAGTAG TACGATGTAG CAACAAGGGC
    TTGAAGGTCT TACCTAAAGG CATTCCAAGA GATGTCACAG AACTGTATCT GGATGGGAAC
    CAGTTTACAC TGGTCCCGAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA
    AGTAACAACA GAATAAGCAC CCTTTCCAAC CAAAGCTTCA GCAACATGAC CCAACTTCTC
    ACCTTAATTC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGGACCTT TGATGGATTG
    AAATCTCTTC GTTTACTGTC TCTACATGGA AATGACATTT CTGTCGTGCC TGAAGGTGCC
    TTTGGTGACC TTTCAGCCTT GTCACACTTA GCAATTGGAG CCAACCCTCT TTACTGTGAT
    TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCC
    CGCTGTGCCG GTCCCGGAGA AATGGCAGAT AAATTGTTAC TCACAACTCC CTCCAAAAAA
    TTTACATGTC AAGGGCTCCC AGGGCCCTGT GGCTTCCTAA TCCCAGGTCC TGTGGATGTT
    ACTATTCAAG CCAAGTGTAA CCCCTGCTTG TCAAATCCAT GTAAAAATGA TGGCACCTGT
    AACAATGACC CGGTGGATTT TTATCGATGC ACCTGCCCAT ATGGTTTCAA GGGCCAGGAC
    TGTGATGTCC CCATTCATGC CTGTATCAGT AATCCATGTA AACATGGAGG AACTTGCCAC
    TTAAAAGAAG GAGAGAATGA TGGATTCTGG TGTACTTGTG CTGATGGGTT TGAAGGAGAA
    AGCTGTGACA TCAATATTGA TGATTGCGAA GATAATGATT GTGAAAATAA TTCTACATGC
    GTTGATGGAA TTAACAACTA CACGTGTCTT TGCCCACCGG AATACACAGC TGCTAATCTG
    AATGAGGTGG AAAAAGGCGA ACTGTGTGAG GAAAAACTGG ACTTCTGTGC ACAAGACCTG
    AATCCCTGCC AGCATGACTC CAAGTGCATC CTGACGCCAA AGGGATTCAA GTGTGACTGC
    ACTCCGGGAT ACATTGGTGA GCACTGTGAC ATCGACTTTG ATGACTGCCA AGATAACAAG
    TGCAAAAACG GTGCTCATTG CACAGATGCA GTGAACGGAT ACACATGTGT CTGTCCTGAA
    GGCTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG CACCAGCCCC
    TGTGATAATT TTGATTGTCA GAATGGAGCC CAGTGTATCA TCAGGGTGAA TGAACCAATA
    TGCCAGTGTT TGCCTGGCTA CTTGGGAGAG AAGTGTGAGA AATTGGTCAG TGTGAATTTT
    GTAAACAAAG AGTCCTATCT TCAGATTCCT TCAGCCAAGG TTCGACCTCA GACAAACATC
    ACACTTCAGA TTGCCACAGA TGAAGACAGC GGCATCCTCT TGTACAAGGG TGACAAGGAC
    CACATTGCTG TGGAACTCTA TCGAGGGCGA GTTCGAGCCA GCTATGACAC CGGCTCTCAC
    CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTAGAG
    CTACTGACCC TGGATTCGAG TCTTTCCCTC TCTGTGGATG GAGGAAGCCC TAAAATCATC
    ACCAATTTGT CAAAACAATC TACTCTGAAT TTCGACTCTC CACTTTACGT AGGAGGTATG
    CCTGGGAAAA ATAACGTGGC TTCGCTGCGC CAGGCCCCTG GGCAGAACGG CACCAGCTTC
    CATGGCTGTA TCCGGAACCT TTACATTAAC AGTGAACTGC AGGACTTCCG GAAAGTGCCT
    ATGCAAACCG GAATTCTGCC TGGCTGTGAA CCATGCCACA AGAAAGTGTG TGCCCATGGC
    ACATGCCAGC CCAGCAGCCA ATCAGGCTTC ACCTGTGAAT GTGAGGAAGG GTGGATGGGG
    CCCCTCTGTG ACCAGAGAAC CAATGATCCC TGTCTCGGAA ACAAATGTGT ACATGGGACC
    TGCTTGCCCA TCAACGCCTT CTCCTACAGC TGCAAGTGCC TGGAGGGCCA CGGCGGGGTC
    CTCTGTGATG AAGAAGAAGA TCTGTTTAAC CCCTGCCAGG TGATCAAGTG CAAGCACGGG
    AAGTGCAGGC TCTCTGGGCT CGGGCAGCCC TATTGTGAAT GCAGCAGTGG ATTCACCGGG
    GACAGCTGTG ACAGAGAAAT TTCTTGTCGA GGGGAACGGA TAAGGGATTA TTACCAAAAG
    CAGCAGGGTT ACGCTGCCTG TCAAACGACT AAGAAAGTAT CTCGCTTGGA GTGCAGAGGC
    GGGTGTGCTG GGGGGCAGTG CTGTGGACCT CTGAGAAGCA AGAGGCGGAA ATACTCTTTC
    GAATGCACAG ATGGATCTTC ATTTGTGGAC GAGGTCGAGA AGGTGGTGAA GTGCGGCTGC
    ACGAGATGTG CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58350
    Clone ID Related Accession (Same CDS sequence) XM_017599275.1
    Accession Version XM_017599275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAAAAAT GTCGCTGTGA AGGGACCACA
    GTAGACTGCT CCAATCAAAA ACTCAACAAA ATCCCAGACC ATATTCCCCA GTACACAGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA
    CTTCCTCAAT TGCGTAAAAT CAACCTTAGC AACAATAAGA TCACTGATAT CGAGGAGGGG
    GCATTCGAAG GTGCGTCTGG TGTGAATGAG ATTCTGCTTA CCAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA AGGATTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT
    CGAATAAGCT GTGTGGGAAA CGACAGTTTC ACAGGACTCG GTTCTGTGCG TCTGCTCTCT
    TTATATGACA ATCAAATTAC CACAGTTGCA CCAGGAGCAT TTGGTACTCT CCATTCATTA
    TCTACACTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTTGGA
    GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC
    TTGAAGGAAA TACCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGACGGAAAC
    GATGATAATA GCTGCTCTCC ACTCTCCCGT TGTCCTTCGG AATGTACTTG CTTGGATACA
    GTAGTACGAT GTAGCAACAA GGGCTTGAAG GTCTTACCTA AAGGCATTCC AAGAGATGTC
    ACAGAACTGT ATCTGGATGG GAACCAGTTT ACACTGGTCC CGAAGGAACT CTCCAACTAC
    AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTTTC CAACCAAAGC
    TTCAGCAACA TGACCCAACT TCTCACCTTA ATTCTCAGTT ACAACCGTCT GAGATGTATC
    CCTCCACGGA CCTTTGATGG ATTGAAATCT CTTCGTTTAC TGTCTCTACA TGGAAATGAC
    ATTTCTGTCG TGCCTGAAGG TGCCTTTGGT GACCTTTCAG CCTTGTCACA CTTAGCAATT
    GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG
    GAATATAAGG AACCTGGAAT TGCCCGCTGT GCCGGTCCCG GAGAAATGGC AGATAAATTG
    TTACTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATGT TACTATTCAA
    GCCAAGTGTA ACCCCTGCTT GTCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC
    CCGGTGGATT TTTATCGATG CACCTGCCCA TATGGTTTCA AGGGCCAGGA CTGTGATGTC
    CCCATTCATG CCTGTATCAG TAATCCATGT AAACATGGAG GAACTTGCCA CTTAAAAGAA
    GGAGAGAATG ATGGATTCTG GTGTACTTGT GCTGATGGGT TTGAAGGAGA AAGCTGTGAC
    ATCAATATTG ATGATTGCGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA
    ATTAACAACT ACACGTGTCT TTGCCCACCG GAATACACAG CTGCTAATCT GAATGAGGTG
    GAAAAAGGCG AACTGTGTGA GGAAAAACTG GACTTCTGTG CACAAGACCT GAATCCCTGC
    CAGCATGACT CCAAGTGCAT CCTGACGCCA AAGGGATTCA AGTGTGACTG CACTCCGGGA
    TACATTGGTG AGCACTGTGA CATCGACTTT GATGACTGCC AAGATAACAA GTGCAAAAAC
    GGTGCTCATT GCACAGATGC AGTGAACGGA TACACATGTG TCTGTCCTGA AGGCTACAGT
    GGCTTGTTCT GTGAGTTTTC TCCACCCATG GTCCTCCCTC GCACCAGCCC CTGTGATAAT
    TTTGATTGTC AGAATGGAGC CCAGTGTATC ATCAGGGTGA ATGAACCAAT ATGCCAGTGT
    TTGCCTGGCT ACTTGGGAGA GAAGTGTGAG AAATTGGTCA GTGTGAATTT TGTAAACAAA
    GAGTCCTATC TTCAGATTCC TTCAGCCAAG GTTCGACCTC AGACAAACAT CACACTTCAG
    ATTGCCACAG ATGAAGACAG CGGCATCCTC TTGTACAAGG GTGACAAGGA CCACATTGCT
    GTGGAACTCT ATCGAGGGCG AGTTCGAGCC AGCTATGACA CCGGCTCTCA CCCGGCTTCT
    GCCATTTACA GTGTGGAGAC AATCAATGAT GGAAACTTCC ACATTGTAGA GCTACTGACC
    CTGGATTCGA GTCTTTCCCT CTCTGTGGAT GGAGGAAGCC CTAAAATCAT CACCAATTTG
    TCAAAACAAT CTACTCTGAA TTTCGACTCT CCACTTTACG TAGGAGGTAT GCCTGGGAAA
    AATAACGTGG CTTCGCTGCG CCAGGCCCCT GGGCAGAACG GCACCAGCTT CCATGGCTGT
    ATCCGGAACC TTTACATTAA CAGTGAACTG CAGGACTTCC GGAAAGTGCC TATGCAAACC
    GGAATTCTGC CTGGCTGTGA ACCATGCCAC AAGAAAGTGT GTGCCCATGG CACATGCCAG
    CCCAGCAGCC AATCAGGCTT CACCTGTGAA TGTGAGGAAG GGTGGATGGG GCCCCTCTGT
    GACCAGAGAA CCAATGATCC CTGTCTCGGA AACAAATGTG TACATGGGAC CTGCTTGCCC
    ATCAACGCCT TCTCCTACAG CTGCAAGTGC CTGGAGGGCC ACGGCGGGGT CCTCTGTGAT
    GAAGAAGAAG ATCTGTTTAA CCCCTGCCAG GTGATCAAGT GCAAGCACGG GAAGTGCAGG
    CTCTCTGGGC TCGGGCAGCC CTATTGTGAA TGCAGCAGTG GATTCACCGG GGACAGCTGT
    GACAGAGAAA TTTCTTGTCG AGGGGAACGG ATAAGGGATT ATTACCAAAA GCAGCAGGGT
    TACGCTGCCT GTCAAACGAC TAAGAAAGTA TCTCGCTTGG AGTGCAGAGG CGGGTGTGCT
    GGGGGGCAGT GCTGTGGACC TCTGAGAAGC AAGAGGCGGA AATACTCTTT CGAATGCACA
    GATGGATCTT CATTTGTGGA CGAGGTCGAG AAGGTGGTGA AGTGCGGCTG CACGAGATGT
    GCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454764.1
    CDS1650..6278
    Translation

    Target ORF information:

    RefSeq Version XM_017599275.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAAAAAT GTCGCTGTGA AGGGACCACA
    GTAGACTGCT CCAATCAAAA ACTCAACAAA ATCCCAGACC ATATTCCCCA GTACACAGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA
    CTTCCTCAAT TGCGTAAAAT CAACCTTAGC AACAATAAGA TCACTGATAT CGAGGAGGGG
    GCATTCGAAG GTGCGTCTGG TGTGAATGAG ATTCTGCTTA CCAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA AGGATTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT
    CGAATAAGCT GTGTGGGAAA CGACAGTTTC ACAGGACTCG GTTCTGTGCG TCTGCTCTCT
    TTATATGACA ATCAAATTAC CACAGTTGCA CCAGGAGCAT TTGGTACTCT CCATTCATTA
    TCTACACTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTTGGA
    GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC
    TTGAAGGAAA TACCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGACGGAAAC
    GATGATAATA GCTGCTCTCC ACTCTCCCGT TGTCCTTCGG AATGTACTTG CTTGGATACA
    GTAGTACGAT GTAGCAACAA GGGCTTGAAG GTCTTACCTA AAGGCATTCC AAGAGATGTC
    ACAGAACTGT ATCTGGATGG GAACCAGTTT ACACTGGTCC CGAAGGAACT CTCCAACTAC
    AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTTTC CAACCAAAGC
    TTCAGCAACA TGACCCAACT TCTCACCTTA ATTCTCAGTT ACAACCGTCT GAGATGTATC
    CCTCCACGGA CCTTTGATGG ATTGAAATCT CTTCGTTTAC TGTCTCTACA TGGAAATGAC
    ATTTCTGTCG TGCCTGAAGG TGCCTTTGGT GACCTTTCAG CCTTGTCACA CTTAGCAATT
    GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG
    GAATATAAGG AACCTGGAAT TGCCCGCTGT GCCGGTCCCG GAGAAATGGC AGATAAATTG
    TTACTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATGT TACTATTCAA
    GCCAAGTGTA ACCCCTGCTT GTCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC
    CCGGTGGATT TTTATCGATG CACCTGCCCA TATGGTTTCA AGGGCCAGGA CTGTGATGTC
    CCCATTCATG CCTGTATCAG TAATCCATGT AAACATGGAG GAACTTGCCA CTTAAAAGAA
    GGAGAGAATG ATGGATTCTG GTGTACTTGT GCTGATGGGT TTGAAGGAGA AAGCTGTGAC
    ATCAATATTG ATGATTGCGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA
    ATTAACAACT ACACGTGTCT TTGCCCACCG GAATACACAG CTGCTAATCT GAATGAGGTG
    GAAAAAGGCG AACTGTGTGA GGAAAAACTG GACTTCTGTG CACAAGACCT GAATCCCTGC
    CAGCATGACT CCAAGTGCAT CCTGACGCCA AAGGGATTCA AGTGTGACTG CACTCCGGGA
    TACATTGGTG AGCACTGTGA CATCGACTTT GATGACTGCC AAGATAACAA GTGCAAAAAC
    GGTGCTCATT GCACAGATGC AGTGAACGGA TACACATGTG TCTGTCCTGA AGGCTACAGT
    GGCTTGTTCT GTGAGTTTTC TCCACCCATG GTCCTCCCTC GCACCAGCCC CTGTGATAAT
    TTTGATTGTC AGAATGGAGC CCAGTGTATC ATCAGGGTGA ATGAACCAAT ATGCCAGTGT
    TTGCCTGGCT ACTTGGGAGA GAAGTGTGAG AAATTGGTCA GTGTGAATTT TGTAAACAAA
    GAGTCCTATC TTCAGATTCC TTCAGCCAAG GTTCGACCTC AGACAAACAT CACACTTCAG
    ATTGCCACAG ATGAAGACAG CGGCATCCTC TTGTACAAGG GTGACAAGGA CCACATTGCT
    GTGGAACTCT ATCGAGGGCG AGTTCGAGCC AGCTATGACA CCGGCTCTCA CCCGGCTTCT
    GCCATTTACA GTGTGGAGAC AATCAATGAT GGAAACTTCC ACATTGTAGA GCTACTGACC
    CTGGATTCGA GTCTTTCCCT CTCTGTGGAT GGAGGAAGCC CTAAAATCAT CACCAATTTG
    TCAAAACAAT CTACTCTGAA TTTCGACTCT CCACTTTACG TAGGAGGTAT GCCTGGGAAA
    AATAACGTGG CTTCGCTGCG CCAGGCCCCT GGGCAGAACG GCACCAGCTT CCATGGCTGT
    ATCCGGAACC TTTACATTAA CAGTGAACTG CAGGACTTCC GGAAAGTGCC TATGCAAACC
    GGAATTCTGC CTGGCTGTGA ACCATGCCAC AAGAAAGTGT GTGCCCATGG CACATGCCAG
    CCCAGCAGCC AATCAGGCTT CACCTGTGAA TGTGAGGAAG GGTGGATGGG GCCCCTCTGT
    GACCAGAGAA CCAATGATCC CTGTCTCGGA AACAAATGTG TACATGGGAC CTGCTTGCCC
    ATCAACGCCT TCTCCTACAG CTGCAAGTGC CTGGAGGGCC ACGGCGGGGT CCTCTGTGAT
    GAAGAAGAAG ATCTGTTTAA CCCCTGCCAG GTGATCAAGT GCAAGCACGG GAAGTGCAGG
    CTCTCTGGGC TCGGGCAGCC CTATTGTGAA TGCAGCAGTG GATTCACCGG GGACAGCTGT
    GACAGAGAAA TTTCTTGTCG AGGGGAACGG ATAAGGGATT ATTACCAAAA GCAGCAGGGT
    TACGCTGCCT GTCAAACGAC TAAGAAAGTA TCTCGCTTGG AGTGCAGAGG CGGGTGTGCT
    GGGGGGCAGT GCTGTGGACC TCTGAGAAGC AAGAGGCGGA AATACTCTTT CGAATGCACA
    GATGGATCTT CATTTGTGGA CGAGGTCGAG AAGGTGGTGA AGTGCGGCTG CACGAGATGT
    GCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58351
    Clone ID Related Accession (Same CDS sequence) XM_017599276.1
    Accession Version XM_017599276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAAAAAT GTCGCTGTGA AGGGACCACA
    GTAGACTGCT CCAATCAAAA ACTCAACAAA ATCCCAGACC ATATTCCCCA GTACACAGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA
    CTTCCTCAAT TGCGTAAAAT CAACCTTAGC AACAATAAGA TCACTGATAT CGAGGAGGGG
    GCATTCGAAG GTGCGTCTGG TGTGAATGAG ATTCTGCTTA CCAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA AGGATTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT
    CGAATAAGCT GTGTGGGAAA CGACAGTTTC ACAGGACTCG GTTCTGTGCG TCTGCTCTCT
    TTATATGACA ATCAAATTAC CACAGTTGCA CCAGGAGCAT TTGGTACTCT CCATTCATTA
    TCTACACTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTTGGA
    GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC
    TTGAAGGAAA TACCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGACGGAAAC
    GATGATAATA GCTGCTCTCC ACTCTCCCGT TGTCCTTCGG AATGTACTTG CTTGGATACA
    GTAGTACGAT GTAGCAACAA GGGCTTGAAG GTCTTACCTA AAGGCATTCC AAGAGATGTC
    ACAGAACTGT ATCTGGATGG GAACCAGTTT ACACTGGTCC CGAAGGAACT CTCCAACTAC
    AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTTTC CAACCAAAGC
    TTCAGCAACA TGACCCAACT TCTCACCTTA ATTCTCAGTT ACAACCGTCT GAGATGTATC
    CCTCCACGGA CCTTTGATGG ATTGAAATCT CTTCGTTTAC TGTCTCTACA TGGAAATGAC
    ATTTCTGTCG TGCCTGAAGG TGCCTTTGGT GACCTTTCAG CCTTGTCACA CTTAGCAATT
    GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG
    GAATATAAGG AACCTGGAAT TGCCCGCTGT GCCGGTCCCG GAGAAATGGC AGATAAATTG
    TTACTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATGT TACTATTCAA
    GCCAAGTGTA ACCCCTGCTT GTCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC
    CCGGTGGATT TTTATCGATG CACCTGCCCA TATGGTTTCA AGGGCCAGGA CTGTGATGTC
    CCCATTCATG CCTGTATCAG TAATCCATGT AAACATGGAG GAACTTGCCA CTTAAAAGAA
    GGAGAGAATG ATGGATTCTG GTGTACTTGT GCTGATGGGT TTGAAGGAGA AAGCTGTGAC
    ATCAATATTG ATGATTGCGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA
    ATTAACAACT ACACGTGTCT TTGCCCACCG GAATACACAG GCGAACTGTG TGAGGAAAAA
    CTGGACTTCT GTGCACAAGA CCTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACG
    CCAAAGGGAT TCAAGTGTGA CTGCACTCCG GGATACATTG GTGAGCACTG TGACATCGAC
    TTTGATGACT GCCAAGATAA CAAGTGCAAA AACGGTGCTC ATTGCACAGA TGCAGTGAAC
    GGATACACAT GTGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC
    ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCCCAGTGT
    ATCATCAGGG TGAATGAACC AATATGCCAG TGTTTGCCTG GCTACTTGGG AGAGAAGTGT
    GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC
    AAGGTTCGAC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC
    CTCTTGTACA AGGGTGACAA GGACCACATT GCTGTGGAAC TCTATCGAGG GCGAGTTCGA
    GCCAGCTATG ACACCGGCTC TCACCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT
    GATGGAAACT TCCACATTGT AGAGCTACTG ACCCTGGATT CGAGTCTTTC CCTCTCTGTG
    GATGGAGGAA GCCCTAAAAT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC
    TCTCCACTTT ACGTAGGAGG TATGCCTGGG AAAAATAACG TGGCTTCGCT GCGCCAGGCC
    CCTGGGCAGA ACGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAA
    CTGCAGGACT TCCGGAAAGT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC
    CACAAGAAAG TGTGTGCCCA TGGCACATGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT
    GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGTCTC
    GGAAACAAAT GTGTACATGG GACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGCAAG
    TGCCTGGAGG GCCACGGCGG GGTCCTCTGT GATGAAGAAG AAGATCTGTT TAACCCCTGC
    CAGGTGATCA AGTGCAAGCA CGGGAAGTGC AGGCTCTCTG GGCTCGGGCA GCCCTATTGT
    GAATGCAGCA GTGGATTCAC CGGGGACAGC TGTGACAGAG AAATTTCTTG TCGAGGGGAA
    CGGATAAGGG ATTATTACCA AAAGCAGCAG GGTTACGCTG CCTGTCAAAC GACTAAGAAA
    GTATCTCGCT TGGAGTGCAG AGGCGGGTGT GCTGGGGGGC AGTGCTGTGG ACCTCTGAGA
    AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGAT CTTCATTTGT GGACGAGGTC
    GAGAAGGTGG TGAAGTGCGG CTGCACGAGA TGTGCCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454765.1
    CDS1650..6251
    Translation

    Target ORF information:

    RefSeq Version XM_017599276.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG CTAAAGAGCA GTATTTCATT CCAGGTACAG AAGATTATCG ATCAAAATTA
    AGTGGAGACT GCTTTGCAGA CTTGGCTTGT CCTGAAAAAT GTCGCTGTGA AGGGACCACA
    GTAGACTGCT CCAATCAAAA ACTCAACAAA ATCCCAGACC ATATTCCCCA GTACACAGCA
    GAGCTGCGTC TCAATAATAA TGAATTCACA GTGTTAGAAG CCACGGGAAT ATTTAAGAAA
    CTTCCTCAAT TGCGTAAAAT CAACCTTAGC AACAATAAGA TCACTGATAT CGAGGAGGGG
    GCATTCGAAG GTGCGTCTGG TGTGAATGAG ATTCTGCTTA CCAGTAACCG TTTGGAAAAT
    GTTCAGCATA AGATGTTCAA AGGATTGGAG AGCCTCAAAA CATTGATGCT GAGAAGTAAT
    CGAATAAGCT GTGTGGGAAA CGACAGTTTC ACAGGACTCG GTTCTGTGCG TCTGCTCTCT
    TTATATGACA ATCAAATTAC CACAGTTGCA CCAGGAGCAT TTGGTACTCT CCATTCATTA
    TCTACACTAA ACCTCTTGGC CAATCCTTTC AACTGTAACT GTCACCTGGC ATGGCTTGGA
    GAATGGCTCA GAAGGAAAAG AATTGTAACA GGAAATCCTC GATGCCAAAA ACCCTACTTC
    TTGAAGGAAA TACCAATCCA GGATGTAGCC ATTCAGGACT TCACCTGTGA TGACGGAAAC
    GATGATAATA GCTGCTCTCC ACTCTCCCGT TGTCCTTCGG AATGTACTTG CTTGGATACA
    GTAGTACGAT GTAGCAACAA GGGCTTGAAG GTCTTACCTA AAGGCATTCC AAGAGATGTC
    ACAGAACTGT ATCTGGATGG GAACCAGTTT ACACTGGTCC CGAAGGAACT CTCCAACTAC
    AAACATTTAA CACTTATAGA CTTAAGTAAC AACAGAATAA GCACCCTTTC CAACCAAAGC
    TTCAGCAACA TGACCCAACT TCTCACCTTA ATTCTCAGTT ACAACCGTCT GAGATGTATC
    CCTCCACGGA CCTTTGATGG ATTGAAATCT CTTCGTTTAC TGTCTCTACA TGGAAATGAC
    ATTTCTGTCG TGCCTGAAGG TGCCTTTGGT GACCTTTCAG CCTTGTCACA CTTAGCAATT
    GGAGCCAACC CTCTTTACTG TGATTGTAAC ATGCAGTGGT TATCCGACTG GGTGAAGTCG
    GAATATAAGG AACCTGGAAT TGCCCGCTGT GCCGGTCCCG GAGAAATGGC AGATAAATTG
    TTACTCACAA CTCCCTCCAA AAAATTTACA TGTCAAGGTC CTGTGGATGT TACTATTCAA
    GCCAAGTGTA ACCCCTGCTT GTCAAATCCA TGTAAAAATG ATGGCACCTG TAACAATGAC
    CCGGTGGATT TTTATCGATG CACCTGCCCA TATGGTTTCA AGGGCCAGGA CTGTGATGTC
    CCCATTCATG CCTGTATCAG TAATCCATGT AAACATGGAG GAACTTGCCA CTTAAAAGAA
    GGAGAGAATG ATGGATTCTG GTGTACTTGT GCTGATGGGT TTGAAGGAGA AAGCTGTGAC
    ATCAATATTG ATGATTGCGA AGATAATGAT TGTGAAAATA ATTCTACATG CGTTGATGGA
    ATTAACAACT ACACGTGTCT TTGCCCACCG GAATACACAG GCGAACTGTG TGAGGAAAAA
    CTGGACTTCT GTGCACAAGA CCTGAATCCC TGCCAGCATG ACTCCAAGTG CATCCTGACG
    CCAAAGGGAT TCAAGTGTGA CTGCACTCCG GGATACATTG GTGAGCACTG TGACATCGAC
    TTTGATGACT GCCAAGATAA CAAGTGCAAA AACGGTGCTC ATTGCACAGA TGCAGTGAAC
    GGATACACAT GTGTCTGTCC TGAAGGCTAC AGTGGCTTGT TCTGTGAGTT TTCTCCACCC
    ATGGTCCTCC CTCGCACCAG CCCCTGTGAT AATTTTGATT GTCAGAATGG AGCCCAGTGT
    ATCATCAGGG TGAATGAACC AATATGCCAG TGTTTGCCTG GCTACTTGGG AGAGAAGTGT
    GAGAAATTGG TCAGTGTGAA TTTTGTAAAC AAAGAGTCCT ATCTTCAGAT TCCTTCAGCC
    AAGGTTCGAC CTCAGACAAA CATCACACTT CAGATTGCCA CAGATGAAGA CAGCGGCATC
    CTCTTGTACA AGGGTGACAA GGACCACATT GCTGTGGAAC TCTATCGAGG GCGAGTTCGA
    GCCAGCTATG ACACCGGCTC TCACCCGGCT TCTGCCATTT ACAGTGTGGA GACAATCAAT
    GATGGAAACT TCCACATTGT AGAGCTACTG ACCCTGGATT CGAGTCTTTC CCTCTCTGTG
    GATGGAGGAA GCCCTAAAAT CATCACCAAT TTGTCAAAAC AATCTACTCT GAATTTCGAC
    TCTCCACTTT ACGTAGGAGG TATGCCTGGG AAAAATAACG TGGCTTCGCT GCGCCAGGCC
    CCTGGGCAGA ACGGCACCAG CTTCCATGGC TGTATCCGGA ACCTTTACAT TAACAGTGAA
    CTGCAGGACT TCCGGAAAGT GCCTATGCAA ACCGGAATTC TGCCTGGCTG TGAACCATGC
    CACAAGAAAG TGTGTGCCCA TGGCACATGC CAGCCCAGCA GCCAATCAGG CTTCACCTGT
    GAATGTGAGG AAGGGTGGAT GGGGCCCCTC TGTGACCAGA GAACCAATGA TCCCTGTCTC
    GGAAACAAAT GTGTACATGG GACCTGCTTG CCCATCAACG CCTTCTCCTA CAGCTGCAAG
    TGCCTGGAGG GCCACGGCGG GGTCCTCTGT GATGAAGAAG AAGATCTGTT TAACCCCTGC
    CAGGTGATCA AGTGCAAGCA CGGGAAGTGC AGGCTCTCTG GGCTCGGGCA GCCCTATTGT
    GAATGCAGCA GTGGATTCAC CGGGGACAGC TGTGACAGAG AAATTTCTTG TCGAGGGGAA
    CGGATAAGGG ATTATTACCA AAAGCAGCAG GGTTACGCTG CCTGTCAAAC GACTAAGAAA
    GTATCTCGCT TGGAGTGCAG AGGCGGGTGT GCTGGGGGGC AGTGCTGTGG ACCTCTGAGA
    AGCAAGAGGC GGAAATACTC TTTCGAATGC ACAGATGGAT CTTCATTTGT GGACGAGGTC
    GAGAAGGTGG TGAAGTGCGG CTGCACGAGA TGTGCCTCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58352
    Clone ID Related Accession (Same CDS sequence) XM_017599277.1
    Accession Version XM_017599277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4590bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC
    CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CGATTGCTTG TACCTGTAGC
    AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG TCATCCCTCC AGGAGCATTC
    TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA ACCAGATCTC GGAACTTGCT
    CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG TCCTGTATGG AAATAAAATC
    ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTACT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT
    CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG GCACCTTCTC AGCTCTCAGA
    GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG
    CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGTCCC
    CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAGCAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC
    TTTGCAGACT TGGCTTGTCC TGAAAAATGT CGCTGTGAAG GGACCACAGT AGACTGCTCC
    AATCAAAAAC TCAACAAAAT CCCAGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC
    AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAATTG
    CGTAAAATCA ACCTTAGCAA CAATAAGATC ACTGATATCG AGGAGGGGGC ATTCGAAGGT
    GCGTCTGGTG TGAATGAGAT TCTGCTTACC AGTAACCGTT TGGAAAATGT TCAGCATAAG
    ATGTTCAAAG GATTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT
    GTGGGAAACG ACAGTTTCAC AGGACTCGGT TCTGTGCGTC TGCTCTCTTT ATATGACAAT
    CAAATTACCA CAGTTGCACC AGGAGCATTT GGTACTCTCC ATTCATTATC TACACTAAAC
    CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTTGGAGA ATGGCTCAGA
    AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCTT GAAGGAAATA
    CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ACGGAAACGA TGATAATAGC
    TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA TGTACTTGCT TGGATACAGT AGTACGATGT
    AGCAACAAGG GCTTGAAGGT CTTACCTAAA GGCATTCCAA GAGATGTCAC AGAACTGTAT
    CTGGATGGGA ACCAGTTTAC ACTGGTCCCG AAGGAACTCT CCAACTACAA ACATTTAACA
    CTTATAGACT TAAGTAACAA CAGAATAAGC ACCCTTTCCA ACCAAAGCTT CAGCAACATG
    ACCCAACTTC TCACCTTAAT TCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGGACC
    TTTGATGGAT TGAAATCTCT TCGTTTACTG TCTCTACATG GAAATGACAT TTCTGTCGTG
    CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC TTGTCACACT TAGCAATTGG AGCCAACCCT
    CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA
    CCTGGAATTG CCCGCTGTGC CGGTCCCGGA GAAATGGCAG ATAAATTGTT ACTCACAACT
    CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATGTTA CTATTCAAGC CAAGTGTAAC
    CCCTGCTTGT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC GGTGGATTTT
    TATCGATGCA CCTGCCCATA TGGTTTCAAG GGCCAGGACT GTGATGTCCC CATTCATGCC
    TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGCCACT TAAAAGAAGG AGAGAATGAT
    GGATTCTGGT GTACTTGTGC TGATGGGTTT GAAGGAGAAA GCTGTGACAT CAATATTGAT
    GATTGCGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC
    ACGTGTCTTT GCCCACCGGA ATACACAGGC GAACTGTGTG AGGAAAAACT GGACTTCTGT
    GCACAAGACC TGAATCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACGCC AAAGGGATTC
    AAGTGTGACT GCACTCCGGG ATACATTGGT GAGCACTGTG ACATCGACTT TGATGACTGC
    CAAGATAACA AGTGCAAAAA CGGTGCTCAT TGCACAGATG CAGTGAACGG ATACACATGT
    GTCTGTCCTG AAGGCTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT
    CGCACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CCCAGTGTAT CATCAGGGTG
    AATGAACCAA TATGCCAGTG TTTGCCTGGC TACTTGGGAG AGAAGTGTGA GAAATTGGTC
    AGTGTGAATT TTGTAAACAA AGAGTCCTAT CTTCAGATTC CTTCAGCCAA GGTTCGACCT
    CAGACAAACA TCACACTTCA GATTGCCACA GATGAAGACA GCGGCATCCT CTTGTACAAG
    GGTGACAAGG ACCACATTGC TGTGGAACTC TATCGAGGGC GAGTTCGAGC CAGCTATGAC
    ACCGGCTCTC ACCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC
    CACATTGTAG AGCTACTGAC CCTGGATTCG AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC
    CCTAAAATCA TCACCAATTT GTCAAAACAA TCTACTCTGA ATTTCGACTC TCCACTTTAC
    GTAGGAGGTA TGCCTGGGAA AAATAACGTG GCTTCGCTGC GCCAGGCCCC TGGGCAGAAC
    GGCACCAGCT TCCATGGCTG TATCCGGAAC CTTTACATTA ACAGTGAACT GCAGGACTTC
    CGGAAAGTGC CTATGCAAAC CGGAATTCTG CCTGGCTGTG AACCATGCCA CAAGAAAGTG
    TGTGCCCATG GCACATGCCA GCCCAGCAGC CAATCAGGCT TCACCTGTGA ATGTGAGGAA
    GGGTGGATGG GGCCCCTCTG TGACCAGAGA ACCAATGATC CCTGTCTCGG AAACAAATGT
    GTACATGGGA CCTGCTTGCC CATCAACGCC TTCTCCTACA GCTGCAAGTG CCTGGAGGGC
    CACGGCGGGG TCCTCTGTGA TGAAGAAGAA GATCTGTTTA ACCCCTGCCA GGTGATCAAG
    TGCAAGCACG GGAAGTGCAG GCTCTCTGGG CTCGGGCAGC CCTATTGTGA ATGCAGCAGT
    GGATTCACCG GGGACAGCTG TGACAGAGAA ATTTCTTGTC GAGGGGAACG GATAAGGGAT
    TATTACCAAA AGCAGCAGGG TTACGCTGCC TGTCAAACGA CTAAGAAAGT ATCTCGCTTG
    GAGTGCAGAG GCGGGTGTGC TGGGGGGCAG TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG
    AAATACTCTT TCGAATGCAC AGATGGATCT TCATTTGTGG ACGAGGTCGA GAAGGTGGTG
    AAGTGCGGCT GCACGAGATG TGCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454766.1
    CDS1650..6239
    Translation

    Target ORF information:

    RefSeq Version XM_017599277.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC
    CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CGATTGCTTG TACCTGTAGC
    AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG TCATCCCTCC AGGAGCATTC
    TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA ACCAGATCTC GGAACTTGCT
    CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG TCCTGTATGG AAATAAAATC
    ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTACT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT
    CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG GCACCTTCTC AGCTCTCAGA
    GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG
    CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGTCCC
    CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGCT
    AAAGAGCAGT ATTTCATTCC AGGTACAGAA GATTATCGAT CAAAATTAAG TGGAGACTGC
    TTTGCAGACT TGGCTTGTCC TGAAAAATGT CGCTGTGAAG GGACCACAGT AGACTGCTCC
    AATCAAAAAC TCAACAAAAT CCCAGACCAT ATTCCCCAGT ACACAGCAGA GCTGCGTCTC
    AATAATAATG AATTCACAGT GTTAGAAGCC ACGGGAATAT TTAAGAAACT TCCTCAATTG
    CGTAAAATCA ACCTTAGCAA CAATAAGATC ACTGATATCG AGGAGGGGGC ATTCGAAGGT
    GCGTCTGGTG TGAATGAGAT TCTGCTTACC AGTAACCGTT TGGAAAATGT TCAGCATAAG
    ATGTTCAAAG GATTGGAGAG CCTCAAAACA TTGATGCTGA GAAGTAATCG AATAAGCTGT
    GTGGGAAACG ACAGTTTCAC AGGACTCGGT TCTGTGCGTC TGCTCTCTTT ATATGACAAT
    CAAATTACCA CAGTTGCACC AGGAGCATTT GGTACTCTCC ATTCATTATC TACACTAAAC
    CTCTTGGCCA ATCCTTTCAA CTGTAACTGT CACCTGGCAT GGCTTGGAGA ATGGCTCAGA
    AGGAAAAGAA TTGTAACAGG AAATCCTCGA TGCCAAAAAC CCTACTTCTT GAAGGAAATA
    CCAATCCAGG ATGTAGCCAT TCAGGACTTC ACCTGTGATG ACGGAAACGA TGATAATAGC
    TGCTCTCCAC TCTCCCGTTG TCCTTCGGAA TGTACTTGCT TGGATACAGT AGTACGATGT
    AGCAACAAGG GCTTGAAGGT CTTACCTAAA GGCATTCCAA GAGATGTCAC AGAACTGTAT
    CTGGATGGGA ACCAGTTTAC ACTGGTCCCG AAGGAACTCT CCAACTACAA ACATTTAACA
    CTTATAGACT TAAGTAACAA CAGAATAAGC ACCCTTTCCA ACCAAAGCTT CAGCAACATG
    ACCCAACTTC TCACCTTAAT TCTCAGTTAC AACCGTCTGA GATGTATCCC TCCACGGACC
    TTTGATGGAT TGAAATCTCT TCGTTTACTG TCTCTACATG GAAATGACAT TTCTGTCGTG
    CCTGAAGGTG CCTTTGGTGA CCTTTCAGCC TTGTCACACT TAGCAATTGG AGCCAACCCT
    CTTTACTGTG ATTGTAACAT GCAGTGGTTA TCCGACTGGG TGAAGTCGGA ATATAAGGAA
    CCTGGAATTG CCCGCTGTGC CGGTCCCGGA GAAATGGCAG ATAAATTGTT ACTCACAACT
    CCCTCCAAAA AATTTACATG TCAAGGTCCT GTGGATGTTA CTATTCAAGC CAAGTGTAAC
    CCCTGCTTGT CAAATCCATG TAAAAATGAT GGCACCTGTA ACAATGACCC GGTGGATTTT
    TATCGATGCA CCTGCCCATA TGGTTTCAAG GGCCAGGACT GTGATGTCCC CATTCATGCC
    TGTATCAGTA ATCCATGTAA ACATGGAGGA ACTTGCCACT TAAAAGAAGG AGAGAATGAT
    GGATTCTGGT GTACTTGTGC TGATGGGTTT GAAGGAGAAA GCTGTGACAT CAATATTGAT
    GATTGCGAAG ATAATGATTG TGAAAATAAT TCTACATGCG TTGATGGAAT TAACAACTAC
    ACGTGTCTTT GCCCACCGGA ATACACAGGC GAACTGTGTG AGGAAAAACT GGACTTCTGT
    GCACAAGACC TGAATCCCTG CCAGCATGAC TCCAAGTGCA TCCTGACGCC AAAGGGATTC
    AAGTGTGACT GCACTCCGGG ATACATTGGT GAGCACTGTG ACATCGACTT TGATGACTGC
    CAAGATAACA AGTGCAAAAA CGGTGCTCAT TGCACAGATG CAGTGAACGG ATACACATGT
    GTCTGTCCTG AAGGCTACAG TGGCTTGTTC TGTGAGTTTT CTCCACCCAT GGTCCTCCCT
    CGCACCAGCC CCTGTGATAA TTTTGATTGT CAGAATGGAG CCCAGTGTAT CATCAGGGTG
    AATGAACCAA TATGCCAGTG TTTGCCTGGC TACTTGGGAG AGAAGTGTGA GAAATTGGTC
    AGTGTGAATT TTGTAAACAA AGAGTCCTAT CTTCAGATTC CTTCAGCCAA GGTTCGACCT
    CAGACAAACA TCACACTTCA GATTGCCACA GATGAAGACA GCGGCATCCT CTTGTACAAG
    GGTGACAAGG ACCACATTGC TGTGGAACTC TATCGAGGGC GAGTTCGAGC CAGCTATGAC
    ACCGGCTCTC ACCCGGCTTC TGCCATTTAC AGTGTGGAGA CAATCAATGA TGGAAACTTC
    CACATTGTAG AGCTACTGAC CCTGGATTCG AGTCTTTCCC TCTCTGTGGA TGGAGGAAGC
    CCTAAAATCA TCACCAATTT GTCAAAACAA TCTACTCTGA ATTTCGACTC TCCACTTTAC
    GTAGGAGGTA TGCCTGGGAA AAATAACGTG GCTTCGCTGC GCCAGGCCCC TGGGCAGAAC
    GGCACCAGCT TCCATGGCTG TATCCGGAAC CTTTACATTA ACAGTGAACT GCAGGACTTC
    CGGAAAGTGC CTATGCAAAC CGGAATTCTG CCTGGCTGTG AACCATGCCA CAAGAAAGTG
    TGTGCCCATG GCACATGCCA GCCCAGCAGC CAATCAGGCT TCACCTGTGA ATGTGAGGAA
    GGGTGGATGG GGCCCCTCTG TGACCAGAGA ACCAATGATC CCTGTCTCGG AAACAAATGT
    GTACATGGGA CCTGCTTGCC CATCAACGCC TTCTCCTACA GCTGCAAGTG CCTGGAGGGC
    CACGGCGGGG TCCTCTGTGA TGAAGAAGAA GATCTGTTTA ACCCCTGCCA GGTGATCAAG
    TGCAAGCACG GGAAGTGCAG GCTCTCTGGG CTCGGGCAGC CCTATTGTGA ATGCAGCAGT
    GGATTCACCG GGGACAGCTG TGACAGAGAA ATTTCTTGTC GAGGGGAACG GATAAGGGAT
    TATTACCAAA AGCAGCAGGG TTACGCTGCC TGTCAAACGA CTAAGAAAGT ATCTCGCTTG
    GAGTGCAGAG GCGGGTGTGC TGGGGGGCAG TGCTGTGGAC CTCTGAGAAG CAAGAGGCGG
    AAATACTCTT TCGAATGCAC AGATGGATCT TCATTTGTGG ACGAGGTCGA GAAGGTGGTG
    AAGTGCGGCT GCACGAGATG TGCCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa58353
    Clone ID Related Accession (Same CDS sequence) XM_017599278.1
    Accession Version XM_017599278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4566bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC
    CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CGATTGCTTG TACCTGTAGC
    AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG TCATCCCTCC AGGAGCATTC
    TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA ACCAGATCTC GGAACTTGCT
    CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG TCCTGTATGG AAATAAAATC
    ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTACT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT
    CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG GCACCTTCTC AGCTCTCAGA
    GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG
    CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGTCCC
    CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT
    ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CAGACTTGGC TTGTCCTGAA
    AAATGTCGCT GTGAAGGGAC CACAGTAGAC TGCTCCAATC AAAAACTCAA CAAAATCCCA
    GACCATATTC CCCAGTACAC AGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGTTA
    GAAGCCACGG GAATATTTAA GAAACTTCCT CAATTGCGTA AAATCAACCT TAGCAACAAT
    AAGATCACTG ATATCGAGGA GGGGGCATTC GAAGGTGCGT CTGGTGTGAA TGAGATTCTG
    CTTACCAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAAGGATT GGAGAGCCTC
    AAAACATTGA TGCTGAGAAG TAATCGAATA AGCTGTGTGG GAAACGACAG TTTCACAGGA
    CTCGGTTCTG TGCGTCTGCT CTCTTTATAT GACAATCAAA TTACCACAGT TGCACCAGGA
    GCATTTGGTA CTCTCCATTC ATTATCTACA CTAAACCTCT TGGCCAATCC TTTCAACTGT
    AACTGTCACC TGGCATGGCT TGGAGAATGG CTCAGAAGGA AAAGAATTGT AACAGGAAAT
    CCTCGATGCC AAAAACCCTA CTTCTTGAAG GAAATACCAA TCCAGGATGT AGCCATTCAG
    GACTTCACCT GTGATGACGG AAACGATGAT AATAGCTGCT CTCCACTCTC CCGTTGTCCT
    TCGGAATGTA CTTGCTTGGA TACAGTAGTA CGATGTAGCA ACAAGGGCTT GAAGGTCTTA
    CCTAAAGGCA TTCCAAGAGA TGTCACAGAA CTGTATCTGG ATGGGAACCA GTTTACACTG
    GTCCCGAAGG AACTCTCCAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA
    ATAAGCACCC TTTCCAACCA AAGCTTCAGC AACATGACCC AACTTCTCAC CTTAATTCTC
    AGTTACAACC GTCTGAGATG TATCCCTCCA CGGACCTTTG ATGGATTGAA ATCTCTTCGT
    TTACTGTCTC TACATGGAAA TGACATTTCT GTCGTGCCTG AAGGTGCCTT TGGTGACCTT
    TCAGCCTTGT CACACTTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG
    TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAACCTG GAATTGCCCG CTGTGCCGGT
    CCCGGAGAAA TGGCAGATAA ATTGTTACTC ACAACTCCCT CCAAAAAATT TACATGTCAA
    GGTCCTGTGG ATGTTACTAT TCAAGCCAAG TGTAACCCCT GCTTGTCAAA TCCATGTAAA
    AATGATGGCA CCTGTAACAA TGACCCGGTG GATTTTTATC GATGCACCTG CCCATATGGT
    TTCAAGGGCC AGGACTGTGA TGTCCCCATT CATGCCTGTA TCAGTAATCC ATGTAAACAT
    GGAGGAACTT GCCACTTAAA AGAAGGAGAG AATGATGGAT TCTGGTGTAC TTGTGCTGAT
    GGGTTTGAAG GAGAAAGCTG TGACATCAAT ATTGATGATT GCGAAGATAA TGATTGTGAA
    AATAATTCTA CATGCGTTGA TGGAATTAAC AACTACACGT GTCTTTGCCC ACCGGAATAC
    ACAGGCGAAC TGTGTGAGGA AAAACTGGAC TTCTGTGCAC AAGACCTGAA TCCCTGCCAG
    CATGACTCCA AGTGCATCCT GACGCCAAAG GGATTCAAGT GTGACTGCAC TCCGGGATAC
    ATTGGTGAGC ACTGTGACAT CGACTTTGAT GACTGCCAAG ATAACAAGTG CAAAAACGGT
    GCTCATTGCA CAGATGCAGT GAACGGATAC ACATGTGTCT GTCCTGAAGG CTACAGTGGC
    TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGCA CCAGCCCCTG TGATAATTTT
    GATTGTCAGA ATGGAGCCCA GTGTATCATC AGGGTGAATG AACCAATATG CCAGTGTTTG
    CCTGGCTACT TGGGAGAGAA GTGTGAGAAA TTGGTCAGTG TGAATTTTGT AAACAAAGAG
    TCCTATCTTC AGATTCCTTC AGCCAAGGTT CGACCTCAGA CAAACATCAC ACTTCAGATT
    GCCACAGATG AAGACAGCGG CATCCTCTTG TACAAGGGTG ACAAGGACCA CATTGCTGTG
    GAACTCTATC GAGGGCGAGT TCGAGCCAGC TATGACACCG GCTCTCACCC GGCTTCTGCC
    ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTAGAGCT ACTGACCCTG
    GATTCGAGTC TTTCCCTCTC TGTGGATGGA GGAAGCCCTA AAATCATCAC CAATTTGTCA
    AAACAATCTA CTCTGAATTT CGACTCTCCA CTTTACGTAG GAGGTATGCC TGGGAAAAAT
    AACGTGGCTT CGCTGCGCCA GGCCCCTGGG CAGAACGGCA CCAGCTTCCA TGGCTGTATC
    CGGAACCTTT ACATTAACAG TGAACTGCAG GACTTCCGGA AAGTGCCTAT GCAAACCGGA
    ATTCTGCCTG GCTGTGAACC ATGCCACAAG AAAGTGTGTG CCCATGGCAC ATGCCAGCCC
    AGCAGCCAAT CAGGCTTCAC CTGTGAATGT GAGGAAGGGT GGATGGGGCC CCTCTGTGAC
    CAGAGAACCA ATGATCCCTG TCTCGGAAAC AAATGTGTAC ATGGGACCTG CTTGCCCATC
    AACGCCTTCT CCTACAGCTG CAAGTGCCTG GAGGGCCACG GCGGGGTCCT CTGTGATGAA
    GAAGAAGATC TGTTTAACCC CTGCCAGGTG ATCAAGTGCA AGCACGGGAA GTGCAGGCTC
    TCTGGGCTCG GGCAGCCCTA TTGTGAATGC AGCAGTGGAT TCACCGGGGA CAGCTGTGAC
    AGAGAAATTT CTTGTCGAGG GGAACGGATA AGGGATTATT ACCAAAAGCA GCAGGGTTAC
    GCTGCCTGTC AAACGACTAA GAAAGTATCT CGCTTGGAGT GCAGAGGCGG GTGTGCTGGG
    GGGCAGTGCT GTGGACCTCT GAGAAGCAAG AGGCGGAAAT ACTCTTTCGA ATGCACAGAT
    GGATCTTCAT TTGTGGACGA GGTCGAGAAG GTGGTGAAGT GCGGCTGCAC GAGATGTGCC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017454767.1
    CDS1650..6215
    Translation

    Target ORF information:

    RefSeq Version XM_017599278.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017599278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGGTCAC
    CAGTCATTCA TGGCTCCCTC CTGCAGTGTG CTGCACTGCC CGATTGCTTG TACCTGTAGC
    AACAACATTG TAGACTGCCG AGGGAAAGGT CTCACTGAGA TCCCCACAAA TCTGCCTGAG
    ACCATCACAG AAATACGTTT GGAACAGAAC TCCATAAGGG TCATCCCTCC AGGAGCATTC
    TCACCATACA AAAAGCTTCG ACGACTAGAC CTGAGTAATA ACCAGATCTC GGAACTTGCT
    CCAGATGCCT TCCAAGGACT GCGTTCTCTG AATTCCCTTG TCCTGTATGG AAATAAAATC
    ACAGAACTCC CAAAAAGTTT ATTTGAAGGA CTGTTTTCCT TACAGCTACT ATTATTGAAT
    GCCAACAAGA TAAACTGCCT TCGGGTAGAT GCTTTTCAGG ACCTGCACAA CTTGAACCTT
    CTCTCCTTAT ACGACAATAA GCTTCAGACT GTTGCCAAGG GCACCTTCTC AGCTCTCAGA
    GCCATCCAAA CTATGCATTT GGCCCAGAAT CCTTTCATTT GTGACTGCCA TCTCAAGTGG
    CTAGCGGATT ATCTCCACAC CAACCCAATT GAGACCAGCG GTGCCCGTTG CACCAGTCCC
    CGCCGCCTGG CTAACAAAAG AATTGGACAG ATCAAAAGCA AGAAATTCCG TTGTTCAGGT
    ACAGAAGATT ATCGATCAAA ATTAAGTGGA GACTGCTTTG CAGACTTGGC TTGTCCTGAA
    AAATGTCGCT GTGAAGGGAC CACAGTAGAC TGCTCCAATC AAAAACTCAA CAAAATCCCA
    GACCATATTC CCCAGTACAC AGCAGAGCTG CGTCTCAATA ATAATGAATT CACAGTGTTA
    GAAGCCACGG GAATATTTAA GAAACTTCCT CAATTGCGTA AAATCAACCT TAGCAACAAT
    AAGATCACTG ATATCGAGGA GGGGGCATTC GAAGGTGCGT CTGGTGTGAA TGAGATTCTG
    CTTACCAGTA ACCGTTTGGA AAATGTTCAG CATAAGATGT TCAAAGGATT GGAGAGCCTC
    AAAACATTGA TGCTGAGAAG TAATCGAATA AGCTGTGTGG GAAACGACAG TTTCACAGGA
    CTCGGTTCTG TGCGTCTGCT CTCTTTATAT GACAATCAAA TTACCACAGT TGCACCAGGA
    GCATTTGGTA CTCTCCATTC ATTATCTACA CTAAACCTCT TGGCCAATCC TTTCAACTGT
    AACTGTCACC TGGCATGGCT TGGAGAATGG CTCAGAAGGA AAAGAATTGT AACAGGAAAT
    CCTCGATGCC AAAAACCCTA CTTCTTGAAG GAAATACCAA TCCAGGATGT AGCCATTCAG
    GACTTCACCT GTGATGACGG AAACGATGAT AATAGCTGCT CTCCACTCTC CCGTTGTCCT
    TCGGAATGTA CTTGCTTGGA TACAGTAGTA CGATGTAGCA ACAAGGGCTT GAAGGTCTTA
    CCTAAAGGCA TTCCAAGAGA TGTCACAGAA CTGTATCTGG ATGGGAACCA GTTTACACTG
    GTCCCGAAGG AACTCTCCAA CTACAAACAT TTAACACTTA TAGACTTAAG TAACAACAGA
    ATAAGCACCC TTTCCAACCA AAGCTTCAGC AACATGACCC AACTTCTCAC CTTAATTCTC
    AGTTACAACC GTCTGAGATG TATCCCTCCA CGGACCTTTG ATGGATTGAA ATCTCTTCGT
    TTACTGTCTC TACATGGAAA TGACATTTCT GTCGTGCCTG AAGGTGCCTT TGGTGACCTT
    TCAGCCTTGT CACACTTAGC AATTGGAGCC AACCCTCTTT ACTGTGATTG TAACATGCAG
    TGGTTATCCG ACTGGGTGAA GTCGGAATAT AAGGAACCTG GAATTGCCCG CTGTGCCGGT
    CCCGGAGAAA TGGCAGATAA ATTGTTACTC ACAACTCCCT CCAAAAAATT TACATGTCAA
    GGTCCTGTGG ATGTTACTAT TCAAGCCAAG TGTAACCCCT GCTTGTCAAA TCCATGTAAA
    AATGATGGCA CCTGTAACAA TGACCCGGTG GATTTTTATC GATGCACCTG CCCATATGGT
    TTCAAGGGCC AGGACTGTGA TGTCCCCATT CATGCCTGTA TCAGTAATCC ATGTAAACAT
    GGAGGAACTT GCCACTTAAA AGAAGGAGAG AATGATGGAT TCTGGTGTAC TTGTGCTGAT
    GGGTTTGAAG GAGAAAGCTG TGACATCAAT ATTGATGATT GCGAAGATAA TGATTGTGAA
    AATAATTCTA CATGCGTTGA TGGAATTAAC AACTACACGT GTCTTTGCCC ACCGGAATAC
    ACAGGCGAAC TGTGTGAGGA AAAACTGGAC TTCTGTGCAC AAGACCTGAA TCCCTGCCAG
    CATGACTCCA AGTGCATCCT GACGCCAAAG GGATTCAAGT GTGACTGCAC TCCGGGATAC
    ATTGGTGAGC ACTGTGACAT CGACTTTGAT GACTGCCAAG ATAACAAGTG CAAAAACGGT
    GCTCATTGCA CAGATGCAGT GAACGGATAC ACATGTGTCT GTCCTGAAGG CTACAGTGGC
    TTGTTCTGTG AGTTTTCTCC ACCCATGGTC CTCCCTCGCA CCAGCCCCTG TGATAATTTT
    GATTGTCAGA ATGGAGCCCA GTGTATCATC AGGGTGAATG AACCAATATG CCAGTGTTTG
    CCTGGCTACT TGGGAGAGAA GTGTGAGAAA TTGGTCAGTG TGAATTTTGT AAACAAAGAG
    TCCTATCTTC AGATTCCTTC AGCCAAGGTT CGACCTCAGA CAAACATCAC ACTTCAGATT
    GCCACAGATG AAGACAGCGG CATCCTCTTG TACAAGGGTG ACAAGGACCA CATTGCTGTG
    GAACTCTATC GAGGGCGAGT TCGAGCCAGC TATGACACCG GCTCTCACCC GGCTTCTGCC
    ATTTACAGTG TGGAGACAAT CAATGATGGA AACTTCCACA TTGTAGAGCT ACTGACCCTG
    GATTCGAGTC TTTCCCTCTC TGTGGATGGA GGAAGCCCTA AAATCATCAC CAATTTGTCA
    AAACAATCTA CTCTGAATTT CGACTCTCCA CTTTACGTAG GAGGTATGCC TGGGAAAAAT
    AACGTGGCTT CGCTGCGCCA GGCCCCTGGG CAGAACGGCA CCAGCTTCCA TGGCTGTATC
    CGGAACCTTT ACATTAACAG TGAACTGCAG GACTTCCGGA AAGTGCCTAT GCAAACCGGA
    ATTCTGCCTG GCTGTGAACC ATGCCACAAG AAAGTGTGTG CCCATGGCAC ATGCCAGCCC
    AGCAGCCAAT CAGGCTTCAC CTGTGAATGT GAGGAAGGGT GGATGGGGCC CCTCTGTGAC
    CAGAGAACCA ATGATCCCTG TCTCGGAAAC AAATGTGTAC ATGGGACCTG CTTGCCCATC
    AACGCCTTCT CCTACAGCTG CAAGTGCCTG GAGGGCCACG GCGGGGTCCT CTGTGATGAA
    GAAGAAGATC TGTTTAACCC CTGCCAGGTG ATCAAGTGCA AGCACGGGAA GTGCAGGCTC
    TCTGGGCTCG GGCAGCCCTA TTGTGAATGC AGCAGTGGAT TCACCGGGGA CAGCTGTGAC
    AGAGAAATTT CTTGTCGAGG GGAACGGATA AGGGATTATT ACCAAAAGCA GCAGGGTTAC
    GCTGCCTGTC AAACGACTAA GAAAGTATCT CGCTTGGAGT GCAGAGGCGG GTGTGCTGGG
    GGGCAGTGCT GTGGACCTCT GAGAAGCAAG AGGCGGAAAT ACTCTTTCGA ATGCACAGAT
    GGATCTTCAT TTGTGGACGA GGTCGAGAAG GTGGTGAAGT GCGGCTGCAC GAGATGTGCC
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa06182
    Clone ID Related Accession (Same CDS sequence) NM_022632.2
    Accession Version NM_022632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Rattus norvegicus(Norway rat)
    Product slit homolog 2 protein precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07015614.1 and AABR07015613.1. On or before Nov 18, 2009 this sequence version replaced XM_001057837.1, XM_346464.3, NM_022632.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG
    GCTTGTCCTG AAAAATGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAAACTC
    AACAAAATCC CAGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAATTGCG TAAAATCAAC
    CTTAGCAACA ATAAGATCAC TGATATCGAG GAGGGGGCAT TCGAAGGTGC GTCTGGTGTG
    AATGAGATTC TGCTTACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA
    TTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT GGGAAACGAC
    AGTTTCACAG GACTCGGTTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA
    GTTGCACCAG GAGCATTTGG TACTCTCCAT TCATTATCTA CACTAAACCT CTTGGCCAAT
    CCTTTCAACT GTAACTGTCA CCTGGCATGG CTTGGAGAAT GGCTCAGAAG GAAAAGAATT
    GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCTTGA AGGAAATACC AATCCAGGAT
    GTAGCCATTC AGGACTTCAC CTGTGATGAC GGAAACGATG ATAATAGCTG CTCTCCACTC
    TCCCGTTGTC CTTCGGAATG TACTTGCTTG GATACAGTAG TACGATGTAG CAACAAGGGC
    TTGAAGGTCT TACCTAAAGG CATTCCAAGA GATGTCACAG AACTGTATCT GGATGGGAAC
    CAGTTTACAC TGGTCCCGAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA
    AGTAACAACA GAATAAGCAC CCTTTCCAAC CAAAGCTTCA GCAACATGAC CCAACTTCTC
    ACCTTAATTC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGGACCTT TGATGGATTG
    AAATCTCTTC GTTTACTGTC TCTACATGGA AATGACATTT CTGTCGTGCC TGAAGGTGCC
    TTTGGTGACC TTTCAGCCTT GTCACACTTA GCAATTGGAG CCAACCCTCT TTACTGTGAT
    TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCC
    CGCTGTGCCG GTCCCGGAGA AATGGCAGAT AAATTGTTAC TCACAACTCC CTCCAAAAAA
    TTTACATGTC AAGGTCCTGT GGATGTTACT ATTCAAGCCA AGTGTAACCC CTGCTTGTCA
    AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCGG TGGATTTTTA TCGATGCACC
    TGCCCATATG GTTTCAAGGG CCAGGACTGT GATGTCCCCA TTCATGCCTG TATCAGTAAT
    CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAAGAAGGAG AGAATGATGG ATTCTGGTGT
    ACTTGTGCTG ATGGGTTTGA AGGAGAAAGC TGTGACATCA ATATTGATGA TTGCGAAGAT
    AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC GTGTCTTTGC
    CCACCGGAAT ACACAGGCGA ACTGTGTGAG GAAAAACTGG ACTTCTGTGC ACAAGACCTG
    AATCCCTGCC AGCATGACTC CAAGTGCATC CTGACGCCAA AGGGATTCAA GTGTGACTGC
    ACTCCGGGAT ACATTGGTGA GCACTGTGAC ATCGACTTTG ATGACTGCCA AGATAACAAG
    TGCAAAAACG GTGCTCATTG CACAGATGCA GTGAACGGAT ACACATGTGT CTGTCCTGAA
    GGCTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG CACCAGCCCC
    TGTGATAATT TTGATTGTCA GAATGGAGCC CAGTGTATCA TCAGGGTGAA TGAACCAATA
    TGCCAGTGTT TGCCTGGCTA CTTGGGAGAG AAGTGTGAGA AATTGGTCAG TGTGAATTTT
    GTAAACAAAG AGTCCTATCT TCAGATTCCT TCAGCCAAGG TTCGACCTCA GACAAACATC
    ACACTTCAGA TTGCCACAGA TGAAGACAGC GGCATCCTCT TGTACAAGGG TGACAAGGAC
    CACATTGCTG TGGAACTCTA TCGAGGGCGA GTTCGAGCCA GCTATGACAC CGGCTCTCAC
    CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTAGAG
    CTACTGACCC TGGATTCGAG TCTTTCCCTC TCTGTGGATG GAGGAAGCCC TAAAATCATC
    ACCAATTTGT CAAAACAATC TACTCTGAAT TTCGACTCTC CACTTTACGT AGGAGGTATG
    CCTGGGAAAA ATAACGTGGC TTCGCTGCGC CAGGCCCCTG GGCAGAACGG CACCAGCTTC
    CATGGCTGTA TCCGGAACCT TTACATTAAC AGTGAACTGC AGGACTTCCG GAAAGTGCCT
    ATGCAAACCG GAATTCTGCC TGGCTGTGAA CCATGCCACA AGAAAGTGTG TGCCCATGGC
    ACATGCCAGC CCAGCAGCCA ATCAGGCTTC ACCTGTGAAT GTGAGGAAGG GTGGATGGGG
    CCCCTCTGTG ACCAGAGAAC CAATGATCCC TGTCTCGGAA ACAAATGTGT ACATGGGACC
    TGCTTGCCCA TCAACGCCTT CTCCTACAGC TGCAAGTGCC TGGAGGGCCA CGGCGGGGTC
    CTCTGTGATG AAGAAGAAGA TCTGTTTAAC CCCTGCCAGG TGATCAAGTG CAAGCACGGG
    AAGTGCAGGC TCTCTGGGCT CGGGCAGCCC TATTGTGAAT GCAGCAGTGG ATTCACCGGG
    GACAGCTGTG ACAGAGAAAT TTCTTGTCGA GGGGAACGGA TAAGGGATTA TTACCAAAAG
    CAGCAGGGTT ACGCTGCCTG TCAAACGACT AAGAAAGTAT CTCGCTTGGA GTGCAGAGGC
    GGGTGTGCTG GGGGGCAGTG CTGTGGACCT CTGAGAAGCA AGAGGCGGAA ATACTCTTTC
    GAATGCACAG ATGGATCTTC ATTTGTGGAC GAGGTCGAGA AGGTGGTGAA GTGCGGCTGC
    ACGAGATGTG CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_072154.2
    CDS520..5097
    Translation

    Target ORF information:

    RefSeq Version NM_022632.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus slit guidance ligand 2 (Slit2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_022632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA TTGGCTGGCA GACACTGTCC CTATCTCTGG CGTTAGTGTT GTCGATCTTG 
    AACCAGGTGG CGCCTCAGGC GTGCCCGGCC CAGTGCTCCT GTTCAGGCAG CACAGTGGAC
    TGTCATGGGC TGGCACTGCG CAGTGTGCCC AGGAATATCC CCCGCAACAC GGAGAGACTG
    GATTTGAATG GAAATAACAT CACAAGGATC ACGAAGACAG ATTTTGCGGG TCTCAGACAC
    CTCAGAGTTC TTCAGCTCAT GGAGAACAAG ATCAGCACCA TCGAGAGGGG AGCATTCCAG
    GATCTTAAGG AGCTAGAAAG ACTGCGTTTA AACAGAAATA ACCTTCAGTT GTTTCCTGAG
    CTGCTGTTTC TTGGGACTGC GAAGCTCTAC CGGCTTGATC TCAGTGAAAA TCAGATTCAA
    GCAATTCCAA GGAAGGCTTT CCGTGGTGCA GTTGACATTA AAAATCTGCA GTTGGATTAC
    AACCAGATCA GCTGCATTGA AGATGGGGCA TTCCGAGCTC TGCGAGATCT GGAAGTGCTC
    ACTCTGAACA ATAACAATAT TACTAGACTT TCAGTGGCAA GTTTCAACCA TATGCCTAAA
    CTTAGGACAT TTCGACTCCA CTCCAACAAC CTATACTGCG ACTGCCACCT GGCCTGGCTC
    TCGGACTGGC TTCGCCAAAG GCCACGGGTG GGCTTGTACA CTCAGTGTAT GGGCCCATCC
    CACCTGAGGG GCCATAATGT AGCAGAGGTT CAAAAACGAG AGTTTGTCTG CAGTGATGAG
    GAAGAAGGTC ACCAGTCATT CATGGCTCCC TCCTGCAGTG TGCTGCACTG CCCGATTGCT
    TGTACCTGTA GCAACAACAT TGTAGACTGC CGAGGGAAAG GTCTCACTGA GATCCCCACA
    AATCTGCCTG AGACCATCAC AGAAATACGT TTGGAACAGA ACTCCATAAG GGTCATCCCT
    CCAGGAGCAT TCTCACCATA CAAAAAGCTT CGACGACTAG ACCTGAGTAA TAACCAGATC
    TCGGAACTTG CTCCAGATGC CTTCCAAGGA CTGCGTTCTC TGAATTCCCT TGTCCTGTAT
    GGAAATAAAA TCACAGAACT CCCAAAAAGT TTATTTGAAG GACTGTTTTC CTTACAGCTA
    CTATTATTGA ATGCCAACAA GATAAACTGC CTTCGGGTAG ATGCTTTTCA GGACCTGCAC
    AACTTGAACC TTCTCTCCTT ATACGACAAT AAGCTTCAGA CTGTTGCCAA GGGCACCTTC
    TCAGCTCTCA GAGCCATCCA AACTATGCAT TTGGCCCAGA ATCCTTTCAT TTGTGACTGC
    CATCTCAAGT GGCTAGCGGA TTATCTCCAC ACCAACCCAA TTGAGACCAG CGGTGCCCGT
    TGCACCAGTC CCCGCCGCCT GGCTAACAAA AGAATTGGAC AGATCAAAAG CAAGAAATTC
    CGTTGTTCAG GTACAGAAGA TTATCGATCA AAATTAAGTG GAGACTGCTT TGCAGACTTG
    GCTTGTCCTG AAAAATGTCG CTGTGAAGGG ACCACAGTAG ACTGCTCCAA TCAAAAACTC
    AACAAAATCC CAGACCATAT TCCCCAGTAC ACAGCAGAGC TGCGTCTCAA TAATAATGAA
    TTCACAGTGT TAGAAGCCAC GGGAATATTT AAGAAACTTC CTCAATTGCG TAAAATCAAC
    CTTAGCAACA ATAAGATCAC TGATATCGAG GAGGGGGCAT TCGAAGGTGC GTCTGGTGTG
    AATGAGATTC TGCTTACCAG TAACCGTTTG GAAAATGTTC AGCATAAGAT GTTCAAAGGA
    TTGGAGAGCC TCAAAACATT GATGCTGAGA AGTAATCGAA TAAGCTGTGT GGGAAACGAC
    AGTTTCACAG GACTCGGTTC TGTGCGTCTG CTCTCTTTAT ATGACAATCA AATTACCACA
    GTTGCACCAG GAGCATTTGG TACTCTCCAT TCATTATCTA CACTAAACCT CTTGGCCAAT
    CCTTTCAACT GTAACTGTCA CCTGGCATGG CTTGGAGAAT GGCTCAGAAG GAAAAGAATT
    GTAACAGGAA ATCCTCGATG CCAAAAACCC TACTTCTTGA AGGAAATACC AATCCAGGAT
    GTAGCCATTC AGGACTTCAC CTGTGATGAC GGAAACGATG ATAATAGCTG CTCTCCACTC
    TCCCGTTGTC CTTCGGAATG TACTTGCTTG GATACAGTAG TACGATGTAG CAACAAGGGC
    TTGAAGGTCT TACCTAAAGG CATTCCAAGA GATGTCACAG AACTGTATCT GGATGGGAAC
    CAGTTTACAC TGGTCCCGAA GGAACTCTCC AACTACAAAC ATTTAACACT TATAGACTTA
    AGTAACAACA GAATAAGCAC CCTTTCCAAC CAAAGCTTCA GCAACATGAC CCAACTTCTC
    ACCTTAATTC TCAGTTACAA CCGTCTGAGA TGTATCCCTC CACGGACCTT TGATGGATTG
    AAATCTCTTC GTTTACTGTC TCTACATGGA AATGACATTT CTGTCGTGCC TGAAGGTGCC
    TTTGGTGACC TTTCAGCCTT GTCACACTTA GCAATTGGAG CCAACCCTCT TTACTGTGAT
    TGTAACATGC AGTGGTTATC CGACTGGGTG AAGTCGGAAT ATAAGGAACC TGGAATTGCC
    CGCTGTGCCG GTCCCGGAGA AATGGCAGAT AAATTGTTAC TCACAACTCC CTCCAAAAAA
    TTTACATGTC AAGGTCCTGT GGATGTTACT ATTCAAGCCA AGTGTAACCC CTGCTTGTCA
    AATCCATGTA AAAATGATGG CACCTGTAAC AATGACCCGG TGGATTTTTA TCGATGCACC
    TGCCCATATG GTTTCAAGGG CCAGGACTGT GATGTCCCCA TTCATGCCTG TATCAGTAAT
    CCATGTAAAC ATGGAGGAAC TTGCCACTTA AAAGAAGGAG AGAATGATGG ATTCTGGTGT
    ACTTGTGCTG ATGGGTTTGA AGGAGAAAGC TGTGACATCA ATATTGATGA TTGCGAAGAT
    AATGATTGTG AAAATAATTC TACATGCGTT GATGGAATTA ACAACTACAC GTGTCTTTGC
    CCACCGGAAT ACACAGGCGA ACTGTGTGAG GAAAAACTGG ACTTCTGTGC ACAAGACCTG
    AATCCCTGCC AGCATGACTC CAAGTGCATC CTGACGCCAA AGGGATTCAA GTGTGACTGC
    ACTCCGGGAT ACATTGGTGA GCACTGTGAC ATCGACTTTG ATGACTGCCA AGATAACAAG
    TGCAAAAACG GTGCTCATTG CACAGATGCA GTGAACGGAT ACACATGTGT CTGTCCTGAA
    GGCTACAGTG GCTTGTTCTG TGAGTTTTCT CCACCCATGG TCCTCCCTCG CACCAGCCCC
    TGTGATAATT TTGATTGTCA GAATGGAGCC CAGTGTATCA TCAGGGTGAA TGAACCAATA
    TGCCAGTGTT TGCCTGGCTA CTTGGGAGAG AAGTGTGAGA AATTGGTCAG TGTGAATTTT
    GTAAACAAAG AGTCCTATCT TCAGATTCCT TCAGCCAAGG TTCGACCTCA GACAAACATC
    ACACTTCAGA TTGCCACAGA TGAAGACAGC GGCATCCTCT TGTACAAGGG TGACAAGGAC
    CACATTGCTG TGGAACTCTA TCGAGGGCGA GTTCGAGCCA GCTATGACAC CGGCTCTCAC
    CCGGCTTCTG CCATTTACAG TGTGGAGACA ATCAATGATG GAAACTTCCA CATTGTAGAG
    CTACTGACCC TGGATTCGAG TCTTTCCCTC TCTGTGGATG GAGGAAGCCC TAAAATCATC
    ACCAATTTGT CAAAACAATC TACTCTGAAT TTCGACTCTC CACTTTACGT AGGAGGTATG
    CCTGGGAAAA ATAACGTGGC TTCGCTGCGC CAGGCCCCTG GGCAGAACGG CACCAGCTTC
    CATGGCTGTA TCCGGAACCT TTACATTAAC AGTGAACTGC AGGACTTCCG GAAAGTGCCT
    ATGCAAACCG GAATTCTGCC TGGCTGTGAA CCATGCCACA AGAAAGTGTG TGCCCATGGC
    ACATGCCAGC CCAGCAGCCA ATCAGGCTTC ACCTGTGAAT GTGAGGAAGG GTGGATGGGG
    CCCCTCTGTG ACCAGAGAAC CAATGATCCC TGTCTCGGAA ACAAATGTGT ACATGGGACC
    TGCTTGCCCA TCAACGCCTT CTCCTACAGC TGCAAGTGCC TGGAGGGCCA CGGCGGGGTC
    CTCTGTGATG AAGAAGAAGA TCTGTTTAAC CCCTGCCAGG TGATCAAGTG CAAGCACGGG
    AAGTGCAGGC TCTCTGGGCT CGGGCAGCCC TATTGTGAAT GCAGCAGTGG ATTCACCGGG
    GACAGCTGTG ACAGAGAAAT TTCTTGTCGA GGGGAACGGA TAAGGGATTA TTACCAAAAG
    CAGCAGGGTT ACGCTGCCTG TCAAACGACT AAGAAAGTAT CTCGCTTGGA GTGCAGAGGC
    GGGTGTGCTG GGGGGCAGTG CTGTGGACCT CTGAGAAGCA AGAGGCGGAA ATACTCTTTC
    GAATGCACAG ATGGATCTTC ATTTGTGGAC GAGGTCGAGA AGGTGGTGAA GTGCGGCTGC
    ACGAGATGTG CCTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.