slit2 cDNA ORF clone, Danio rerio(zebrafish)

The following slit2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slit2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa147307 XM_021475863.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa147293 XM_021475849.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147294 XM_021475850.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147295 XM_021475851.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147296 XM_021475852.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147297 XM_021475853.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147298 XM_021475854.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147299 XM_021475855.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147300 XM_021475856.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147301 XM_021475857.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147302 XM_021475858.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147303 XM_021475859.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147304 XM_021475860.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147305 XM_021475861.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa147306 XM_021475862.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa03853 NM_131735.1
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa39428 XM_009291085.2
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa39429 XM_009291088.2
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa39430 XM_009291092.2
Latest version!
Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa147307
    Clone ID Related Accession (Same CDS sequence) XM_021475863.1
    Accession Version XM_021475863.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGCTACA GTGGGTTGTT CTGCGAGTTT TCTCCTCCGA TGGTCCTGCC AAGAACAAGT
    CCATGCGATC ACTACGACTG TGCCAACGGT GCACAGTGTG TTGTGAAGGA CACGGACCCC
    GTGTGTCAGT GTCTGCCAGG TTATGAGGGT GTGCACTGTG AAAGACTGGT CAGCGTCAAC
    TTCATCAACA GAGAATCCTT CCTGCAAATC CCCTCCAACC TCATTACAGA ACAGGCCAAT
    ATATCTCTGC AGATCGCCAC AGATGAGGAT AATGGAGTGT TGCTGTACAA AGGAGATAAT
    GAACACATTG CTGTGGAGCT TTACAGAGGC CGACTGAGAG TCAGTTATGA CTCTGGATCA
    TATCCTCCAT CTGCAATCTA CAGTGTCGAG ACAATAAATG ATGGCAGCTT TCATGTAGTG
    GAGCTGGTGG CAAAGGATCA GTCTCTGTCA CTGTCCATTG ATGGAGGAAG CCCGAAATCC
    ATCAACACTG CCAATAGCCC CAGCCCAGTG CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT
    CTTCCTCAGC AGTCTGGATT GGCCTCGTTG CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT
    TTCCATGGCT GCATCCGAAA TCTCTACATT AATGACCAAC TCCAGGATCT CACCCAGTTT
    TTGCTGCAGG AGGGTGTTGT GCCCGGATGC CAGCCATGCC AGCGGAGCAT GTGTGCTCAT
    GGCCAGTGCC ATGCCACTGG ACAGTCCTCT TTCAGCTGTG AGTGTGAGGC GGGATGGACT
    GGCCCGCTCT GTGACCAGCA GGTCAACAAC CCATGTGACG GGAACAAGTG TATCCATGGC
    TCGTGTATGG CCATAAACTC ATACTCGTAC AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT
    GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC CCCTGTCAGT ATATAGCATG CAAATACGGC
    CGATGTCGTG TGTCTGGACT GGGCAAAGCC TATTGTGAGT GCAACAGCGG CTACACGGGG
    CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG GGGGAACGGG TCCGAGATCA CTATCAGACG
    CAGCAGGGCT ACGCCGCGTG CCAGAGCACA GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC
    AGCTGTGGGG ACGGGACCTC CTGCTGCGCC CCGCTCCGCA GCAAACGCAG GAAATACACC
    TTCCAATGCA CAGACGGGTC CTCTTTTGTA CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC
    TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331538.1
    CDS833..5113
    Translation

    Target ORF information:

    RefSeq Version XM_021475863.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475863.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGCTACA GTGGGTTGTT CTGCGAGTTT TCTCCTCCGA TGGTCCTGCC AAGAACAAGT
    CCATGCGATC ACTACGACTG TGCCAACGGT GCACAGTGTG TTGTGAAGGA CACGGACCCC
    GTGTGTCAGT GTCTGCCAGG TTATGAGGGT GTGCACTGTG AAAGACTGGT CAGCGTCAAC
    TTCATCAACA GAGAATCCTT CCTGCAAATC CCCTCCAACC TCATTACAGA ACAGGCCAAT
    ATATCTCTGC AGATCGCCAC AGATGAGGAT AATGGAGTGT TGCTGTACAA AGGAGATAAT
    GAACACATTG CTGTGGAGCT TTACAGAGGC CGACTGAGAG TCAGTTATGA CTCTGGATCA
    TATCCTCCAT CTGCAATCTA CAGTGTCGAG ACAATAAATG ATGGCAGCTT TCATGTAGTG
    GAGCTGGTGG CAAAGGATCA GTCTCTGTCA CTGTCCATTG ATGGAGGAAG CCCGAAATCC
    ATCAACACTG CCAATAGCCC CAGCCCAGTG CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT
    CTTCCTCAGC AGTCTGGATT GGCCTCGTTG CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT
    TTCCATGGCT GCATCCGAAA TCTCTACATT AATGACCAAC TCCAGGATCT CACCCAGTTT
    TTGCTGCAGG AGGGTGTTGT GCCCGGATGC CAGCCATGCC AGCGGAGCAT GTGTGCTCAT
    GGCCAGTGCC ATGCCACTGG ACAGTCCTCT TTCAGCTGTG AGTGTGAGGC GGGATGGACT
    GGCCCGCTCT GTGACCAGCA GGTCAACAAC CCATGTGACG GGAACAAGTG TATCCATGGC
    TCGTGTATGG CCATAAACTC ATACTCGTAC AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT
    GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC CCCTGTCAGT ATATAGCATG CAAATACGGC
    CGATGTCGTG TGTCTGGACT GGGCAAAGCC TATTGTGAGT GCAACAGCGG CTACACGGGG
    CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG GGGGAACGGG TCCGAGATCA CTATCAGACG
    CAGCAGGGCT ACGCCGCGTG CCAGAGCACA GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC
    AGCTGTGGGG ACGGGACCTC CTGCTGCGCC CCGCTCCGCA GCAAACGCAG GAAATACACC
    TTCCAATGCA CAGACGGGTC CTCTTTTGTA CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC
    TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147293
    Clone ID Related Accession (Same CDS sequence) XM_021475849.1
    Accession Version XM_021475849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGATTTG AGGGTGATGA ATGTGAGATA AACATTGACG ACTGTGAGGA TAATGACTGT
    GAGAATAACT CTACATGCGT GGACGGTATC AACAACTATA CCTGTCTCTG CTCACCTGAA
    TACACAGCCG CTAATAATAA TGAAGTGGAG AAAGGGGAGC TGTGTGAAGA TAAGCTGGAT
    TTTTGTGCTT CAGAGCTTAA CCTGTGTCAG CATGACTCCA AATGTATTCT GACTGCTAAA
    GGCTTCATGT GTGAATGCAC TCCAGGTTAT ACCGGTGAGC ACTGTGAGGT GGATTTTGAT
    GATTGTGAAG ATAATAAGTG TAAAAACGGG GCTCAGTGTA TTGATGCTGT GAACGGCTAC
    ACGTGTGTGT GTCCAGAAGG CTACAGTGGG TTGTTCTGCG AGTTTTCTCC TCCGATGGTC
    CTGCCAAGAA CAAGTCCATG CGATCACTAC GACTGTGCCA ACGGTGCACA GTGTGTTGTG
    AAGGACACGG ACCCCGTGTG TCAGTGTCTG CCAGGTTATG AGGGTGTGCA CTGTGAAAGA
    CTGGTCAGCG TCAACTTCAT CAACAGAGAA TCCTTCCTGC AAATCCCCTC CAACCTCATT
    ACAGAACAGG CCAATATATC TCTGCAGATC GCCACAGATG AGGATAATGG AGTGTTGCTG
    TACAAAGGAG ATAATGAACA CATTGCTGTG GAGCTTTACA GAGGCCGACT GAGAGTCAGT
    TATGACTCTG GATCATATCC TCCATCTGCA ATCTACAGTG TCGAGACAAT AAATGATGGC
    AGCTTTCATG TAGTGGAGCT GGTGGCAAAG GATCAGTCTC TGTCACTGTC CATTGATGGA
    GGAAGCCCGA AATCCATCAA CACTGCCAAT AGCCCCAGCC CAGTGCCCAG CCCGGCCCCG
    CTTTATCTTG GTGGTCTTCC TCAGCAGTCT GGATTGGCCT CGTTGCGTCA GGGCTCTGGC
    AGGAATGGCT CTAGTTTCCA TGGCTGCATC CGAAATCTCT ACATTAATGA CCAACTCCAG
    GATCTCACCC AGTTTTTGCT GCAGGAGGGT GTTGTGCCCG GATGCCAGCC ATGCCAGCGG
    AGCATGTGTG CTCATGGCCA GTGCCATGCC ACTGGACAGT CCTCTTTCAG CTGTGAGTGT
    GAGGCGGGAT GGACTGGCCC GCTCTGTGAC CAGCAGGTCA ACAACCCATG TGACGGGAAC
    AAGTGTATCC ATGGCTCGTG TATGGCCATA AACTCATACT CGTACAGCTG CCGTTGTCTT
    CCAGGCTTTG CAGGTGTGCT GTGTGATGAA GAGGAGCAGC TCAGCCCCTG TCAGTATATA
    GCATGCAAAT ACGGCCGATG TCGTGTGTCT GGACTGGGCA AAGCCTATTG TGAGTGCAAC
    AGCGGCTACA CGGGGCAAAG CTGCGACAGA GAGATGGCGT GTCGGGGGGA ACGGGTCCGA
    GATCACTATC AGACGCAGCA GGGCTACGCC GCGTGCCAGA GCACAGAGAA GGTCTCGCGT
    TTAGAGTGTC GGGGCAGCTG TGGGGACGGG ACCTCCTGCT GCGCCCCGCT CCGCAGCAAA
    CGCAGGAAAT ACACCTTCCA ATGCACAGAC GGGTCCTCTT TTGTACAGGA GGTGGAAAAG
    GTTGTCAAGT GTGGCTGCAC AAAATGTCCA TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331524.1
    CDS827..5482
    Translation

    Target ORF information:

    RefSeq Version XM_021475849.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGATTTG AGGGTGATGA ATGTGAGATA AACATTGACG ACTGTGAGGA TAATGACTGT
    GAGAATAACT CTACATGCGT GGACGGTATC AACAACTATA CCTGTCTCTG CTCACCTGAA
    TACACAGCCG CTAATAATAA TGAAGTGGAG AAAGGGGAGC TGTGTGAAGA TAAGCTGGAT
    TTTTGTGCTT CAGAGCTTAA CCTGTGTCAG CATGACTCCA AATGTATTCT GACTGCTAAA
    GGCTTCATGT GTGAATGCAC TCCAGGTTAT ACCGGTGAGC ACTGTGAGGT GGATTTTGAT
    GATTGTGAAG ATAATAAGTG TAAAAACGGG GCTCAGTGTA TTGATGCTGT GAACGGCTAC
    ACGTGTGTGT GTCCAGAAGG CTACAGTGGG TTGTTCTGCG AGTTTTCTCC TCCGATGGTC
    CTGCCAAGAA CAAGTCCATG CGATCACTAC GACTGTGCCA ACGGTGCACA GTGTGTTGTG
    AAGGACACGG ACCCCGTGTG TCAGTGTCTG CCAGGTTATG AGGGTGTGCA CTGTGAAAGA
    CTGGTCAGCG TCAACTTCAT CAACAGAGAA TCCTTCCTGC AAATCCCCTC CAACCTCATT
    ACAGAACAGG CCAATATATC TCTGCAGATC GCCACAGATG AGGATAATGG AGTGTTGCTG
    TACAAAGGAG ATAATGAACA CATTGCTGTG GAGCTTTACA GAGGCCGACT GAGAGTCAGT
    TATGACTCTG GATCATATCC TCCATCTGCA ATCTACAGTG TCGAGACAAT AAATGATGGC
    AGCTTTCATG TAGTGGAGCT GGTGGCAAAG GATCAGTCTC TGTCACTGTC CATTGATGGA
    GGAAGCCCGA AATCCATCAA CACTGCCAAT AGCCCCAGCC CAGTGCCCAG CCCGGCCCCG
    CTTTATCTTG GTGGTCTTCC TCAGCAGTCT GGATTGGCCT CGTTGCGTCA GGGCTCTGGC
    AGGAATGGCT CTAGTTTCCA TGGCTGCATC CGAAATCTCT ACATTAATGA CCAACTCCAG
    GATCTCACCC AGTTTTTGCT GCAGGAGGGT GTTGTGCCCG GATGCCAGCC ATGCCAGCGG
    AGCATGTGTG CTCATGGCCA GTGCCATGCC ACTGGACAGT CCTCTTTCAG CTGTGAGTGT
    GAGGCGGGAT GGACTGGCCC GCTCTGTGAC CAGCAGGTCA ACAACCCATG TGACGGGAAC
    AAGTGTATCC ATGGCTCGTG TATGGCCATA AACTCATACT CGTACAGCTG CCGTTGTCTT
    CCAGGCTTTG CAGGTGTGCT GTGTGATGAA GAGGAGCAGC TCAGCCCCTG TCAGTATATA
    GCATGCAAAT ACGGCCGATG TCGTGTGTCT GGACTGGGCA AAGCCTATTG TGAGTGCAAC
    AGCGGCTACA CGGGGCAAAG CTGCGACAGA GAGATGGCGT GTCGGGGGGA ACGGGTCCGA
    GATCACTATC AGACGCAGCA GGGCTACGCC GCGTGCCAGA GCACAGAGAA GGTCTCGCGT
    TTAGAGTGTC GGGGCAGCTG TGGGGACGGG ACCTCCTGCT GCGCCCCGCT CCGCAGCAAA
    CGCAGGAAAT ACACCTTCCA ATGCACAGAC GGGTCCTCTT TTGTACAGGA GGTGGAAAAG
    GTTGTCAAGT GTGGCTGCAC AAAATGTCCA TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147294
    Clone ID Related Accession (Same CDS sequence) XM_021475850.1
    Accession Version XM_021475850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GCTAAAGAGC AATATTTTAT ACCAGGGATT
    CATCATATCA ATAAGTGCGT AGAAGACTAC CGCTCAAAAC TAGGTGGGGA TTGCTTTGCT
    GATTTGGCCT GTCCAGAGAA ATGTCGATGT GAAGGAACCA CAGTGGACTG CTCTGGACAA
    AAGCTCACCA AGATCCCAGA CCACATCCCT CAGTATACAG CAGAACTCCG CTTGAATAAT
    AATGAATTCA CAGTGCTGGA GGCTACAGGA ATCTTCAAGA AGCTTCCTCA GTTGCGCAAA
    ATCAATCTTA GCAACAATAA GATCACAGAT ATCGAAGAAG GCACATTTGA AGGGGCCAGT
    GGAGTAAATG AACTCATACT GACCAGCAAT CGCTTGGAGG GGGTTCACTA CAGCATGCTG
    AAAGGCCTCG GTGGTCTCCG CACACTGATG TTGAGGAGTA ACAGGATAAG CTGTGTCAAT
    AACGGCAGTT TTACCGGTCT GAGCTCTGTG AGGTTGCTTT CCCTCTATGA TAACCTGATC
    ACCTCCATGT CCCCAGGAGC CTTTGACACA CTACATTCGC TCTCCACATT GAACTTGTTG
    GCCAATCCGT TTAACTGTAA CTGCCATCTG GCCTGGCTTG GTGAATGGCT CCGCAAGAAA
    CGGATTGTCA CAGGCAACCC CCGCTGTCAG AGCCCCTACT TTCTCAAGGA GATCCCTATT
    CAAGATGTAG CTGTACAAGA CTTTGCCTGT GAAGAAGGTA ACGATGAGAA TAGCTGTTCA
    CCACTGGCTC GCTGTCCTGC CGAGTGTTCG TGTTTGGATA CAGTGGTTCG TTGCAGTAAT
    AAAGGACTCA AGGTTTTGCC TAAGGGCATT CCTAGAGACG TTACAGAGCT CTATCTCGAT
    GGCAACGAGT TCACACAAGT CCCTCTGGAA CTATCCAATT ATAAACACCT CACTCTCATT
    GATTTGAGTA ATAACCAGAT CAGCACATTG TCCAACCACA GCTTCAGTAA CATGTCCGAG
    CTGCTCACAC TAATTTTAAG CTACAACAGA TTAAGGTGTA TTCCTGCAAA AGCGTTCGAC
    GGCCTCAAGT CTCTGCGCTT GTTGTCTCTC CATGGTAATG ATATTGCTGT GATCCCAGAT
    GGAGCTTTCA AAGATCTGTC CTCACTCTCT CACCTGGCTC TGGGCGCAAA CCCCCTGTAC
    TGTGACTGTC ACATGCAGTG GCTCTCTGAC TGGGTAAAGA GTGGATACAA GGAGCCTGGC
    ATCGCCCGCT GTACCGGTCC TGGAGACATG ACTGACAAAC TGCTGCTCAC TACACCCTCT
    AAGAAATTCA CATGCACAGA GGTGCCGGGG ACTTGTGGTT TTCCTGTGCC AGGTCCAGTG
    GATGTAAGCA TTTTGGCTAA ATGTAACCCT TGCTTGTCCA ATCCCTGCAA GAATGATGGG
    ACCTGCAGTA ACCATCCTGT AGATTTTTAC AGATGCACCT GCCCGTATGG TTTTAAGGGT
    CAAGACTGTG AGGAACCAAT TCATGCATGC ATCAGTAACC CCTGTCAGAA TGGAGGAACC
    TGTCATCTGA AGGATGGAGA GGAAAACACT CACTGGTGTG TGTGTCCAGA AGGATTTGAG
    GGTGATGAAT GTGAGATAAA CATTGACGAC TGTGAGGATA ATGACTGTGA GAATAACTCT
    ACATGCGTGG ACGGTATCAA CAACTATACC TGTCTCTGCT CACCTGAATA CACAGCCGCT
    AATAATAATG AAGTGGAGAA AGGGGAGCTG TGTGAAGATA AGCTGGATTT TTGTGCTTCA
    GAGCTTAACC TGTGTCAGCA TGACTCCAAA TGTATTCTGA CTGCTAAAGG CTTCATGTGT
    GAATGCACTC CAGGTTATAC CGGTGAGCAC TGTGAGGTGG ATTTTGATGA TTGTGAAGAT
    AATAAGTGTA AAAACGGGGC TCAGTGTATT GATGCTGTGA ACGGCTACAC GTGTGTGTGT
    CCAGAAGGCT ACAGTGGGTT GTTCTGCGAG TTTTCTCCTC CGATGGTCCT GCCAAGAACA
    AGTCCATGCG ATCACTACGA CTGTGCCAAC GGTGCACAGT GTGTTGTGAA GGACACGGAC
    CCCGTGTGTC AGTGTCTGCC AGGTTATGAG GGTGTGCACT GTGAAAGACT GGTCAGCGTC
    AACTTCATCA ACAGAGAATC CTTCCTGCAA ATCCCCTCCA ACCTCATTAC AGAACAGGCC
    AATATATCTC TGCAGATCGC CACAGATGAG GATAATGGAG TGTTGCTGTA CAAAGGAGAT
    AATGAACACA TTGCTGTGGA GCTTTACAGA GGCCGACTGA GAGTCAGTTA TGACTCTGGA
    TCATATCCTC CATCTGCAAT CTACAGTGTC GAGACAATAA ATGATGGCAG CTTTCATGTA
    GTGGAGCTGG TGGCAAAGGA TCAGTCTCTG TCACTGTCCA TTGATGGAGG AAGCCCGAAA
    TCCATCAACA CTGCCAATAG CCCCAGCCCA GTGCCCAGCC CGGCCCCGCT TTATCTTGGT
    GGTCTTCCTC AGCAGTCTGG ATTGGCCTCG TTGCGTCAGG GCTCTGGCAG GAATGGCTCT
    AGTTTCCATG GCTGCATCCG AAATCTCTAC ATTAATGACC AACTCCAGGA TCTCACCCAG
    TTTTTGCTGC AGGAGGGTGT TGTGCCCGGA TGCCAGCCAT GCCAGCGGAG CATGTGTGCT
    CATGGCCAGT GCCATGCCAC TGGACAGTCC TCTTTCAGCT GTGAGTGTGA GGCGGGATGG
    ACTGGCCCGC TCTGTGACCA GCAGGTCAAC AACCCATGTG ACGGGAACAA GTGTATCCAT
    GGCTCGTGTA TGGCCATAAA CTCATACTCG TACAGCTGCC GTTGTCTTCC AGGCTTTGCA
    GGTGTGCTGT GTGATGAAGA GGAGCAGCTC AGCCCCTGTC AGTATATAGC ATGCAAATAC
    GGCCGATGTC GTGTGTCTGG ACTGGGCAAA GCCTATTGTG AGTGCAACAG CGGCTACACG
    GGGCAAAGCT GCGACAGAGA GATGGCGTGT CGGGGGGAAC GGGTCCGAGA TCACTATCAG
    ACGCAGCAGG GCTACGCCGC GTGCCAGAGC ACAGAGAAGG TCTCGCGTTT AGAGTGTCGG
    GGCAGCTGTG GGGACGGGAC CTCCTGCTGC GCCCCGCTCC GCAGCAAACG CAGGAAATAC
    ACCTTCCAAT GCACAGACGG GTCCTCTTTT GTACAGGAGG TGGAAAAGGT TGTCAAGTGT
    GGCTGCACAA AATGTCCATC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331525.1
    CDS827..5470
    Translation

    Target ORF information:

    RefSeq Version XM_021475850.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GCTAAAGAGC AATATTTTAT ACCAGGGATT
    CATCATATCA ATAAGTGCGT AGAAGACTAC CGCTCAAAAC TAGGTGGGGA TTGCTTTGCT
    GATTTGGCCT GTCCAGAGAA ATGTCGATGT GAAGGAACCA CAGTGGACTG CTCTGGACAA
    AAGCTCACCA AGATCCCAGA CCACATCCCT CAGTATACAG CAGAACTCCG CTTGAATAAT
    AATGAATTCA CAGTGCTGGA GGCTACAGGA ATCTTCAAGA AGCTTCCTCA GTTGCGCAAA
    ATCAATCTTA GCAACAATAA GATCACAGAT ATCGAAGAAG GCACATTTGA AGGGGCCAGT
    GGAGTAAATG AACTCATACT GACCAGCAAT CGCTTGGAGG GGGTTCACTA CAGCATGCTG
    AAAGGCCTCG GTGGTCTCCG CACACTGATG TTGAGGAGTA ACAGGATAAG CTGTGTCAAT
    AACGGCAGTT TTACCGGTCT GAGCTCTGTG AGGTTGCTTT CCCTCTATGA TAACCTGATC
    ACCTCCATGT CCCCAGGAGC CTTTGACACA CTACATTCGC TCTCCACATT GAACTTGTTG
    GCCAATCCGT TTAACTGTAA CTGCCATCTG GCCTGGCTTG GTGAATGGCT CCGCAAGAAA
    CGGATTGTCA CAGGCAACCC CCGCTGTCAG AGCCCCTACT TTCTCAAGGA GATCCCTATT
    CAAGATGTAG CTGTACAAGA CTTTGCCTGT GAAGAAGGTA ACGATGAGAA TAGCTGTTCA
    CCACTGGCTC GCTGTCCTGC CGAGTGTTCG TGTTTGGATA CAGTGGTTCG TTGCAGTAAT
    AAAGGACTCA AGGTTTTGCC TAAGGGCATT CCTAGAGACG TTACAGAGCT CTATCTCGAT
    GGCAACGAGT TCACACAAGT CCCTCTGGAA CTATCCAATT ATAAACACCT CACTCTCATT
    GATTTGAGTA ATAACCAGAT CAGCACATTG TCCAACCACA GCTTCAGTAA CATGTCCGAG
    CTGCTCACAC TAATTTTAAG CTACAACAGA TTAAGGTGTA TTCCTGCAAA AGCGTTCGAC
    GGCCTCAAGT CTCTGCGCTT GTTGTCTCTC CATGGTAATG ATATTGCTGT GATCCCAGAT
    GGAGCTTTCA AAGATCTGTC CTCACTCTCT CACCTGGCTC TGGGCGCAAA CCCCCTGTAC
    TGTGACTGTC ACATGCAGTG GCTCTCTGAC TGGGTAAAGA GTGGATACAA GGAGCCTGGC
    ATCGCCCGCT GTACCGGTCC TGGAGACATG ACTGACAAAC TGCTGCTCAC TACACCCTCT
    AAGAAATTCA CATGCACAGA GGTGCCGGGG ACTTGTGGTT TTCCTGTGCC AGGTCCAGTG
    GATGTAAGCA TTTTGGCTAA ATGTAACCCT TGCTTGTCCA ATCCCTGCAA GAATGATGGG
    ACCTGCAGTA ACCATCCTGT AGATTTTTAC AGATGCACCT GCCCGTATGG TTTTAAGGGT
    CAAGACTGTG AGGAACCAAT TCATGCATGC ATCAGTAACC CCTGTCAGAA TGGAGGAACC
    TGTCATCTGA AGGATGGAGA GGAAAACACT CACTGGTGTG TGTGTCCAGA AGGATTTGAG
    GGTGATGAAT GTGAGATAAA CATTGACGAC TGTGAGGATA ATGACTGTGA GAATAACTCT
    ACATGCGTGG ACGGTATCAA CAACTATACC TGTCTCTGCT CACCTGAATA CACAGCCGCT
    AATAATAATG AAGTGGAGAA AGGGGAGCTG TGTGAAGATA AGCTGGATTT TTGTGCTTCA
    GAGCTTAACC TGTGTCAGCA TGACTCCAAA TGTATTCTGA CTGCTAAAGG CTTCATGTGT
    GAATGCACTC CAGGTTATAC CGGTGAGCAC TGTGAGGTGG ATTTTGATGA TTGTGAAGAT
    AATAAGTGTA AAAACGGGGC TCAGTGTATT GATGCTGTGA ACGGCTACAC GTGTGTGTGT
    CCAGAAGGCT ACAGTGGGTT GTTCTGCGAG TTTTCTCCTC CGATGGTCCT GCCAAGAACA
    AGTCCATGCG ATCACTACGA CTGTGCCAAC GGTGCACAGT GTGTTGTGAA GGACACGGAC
    CCCGTGTGTC AGTGTCTGCC AGGTTATGAG GGTGTGCACT GTGAAAGACT GGTCAGCGTC
    AACTTCATCA ACAGAGAATC CTTCCTGCAA ATCCCCTCCA ACCTCATTAC AGAACAGGCC
    AATATATCTC TGCAGATCGC CACAGATGAG GATAATGGAG TGTTGCTGTA CAAAGGAGAT
    AATGAACACA TTGCTGTGGA GCTTTACAGA GGCCGACTGA GAGTCAGTTA TGACTCTGGA
    TCATATCCTC CATCTGCAAT CTACAGTGTC GAGACAATAA ATGATGGCAG CTTTCATGTA
    GTGGAGCTGG TGGCAAAGGA TCAGTCTCTG TCACTGTCCA TTGATGGAGG AAGCCCGAAA
    TCCATCAACA CTGCCAATAG CCCCAGCCCA GTGCCCAGCC CGGCCCCGCT TTATCTTGGT
    GGTCTTCCTC AGCAGTCTGG ATTGGCCTCG TTGCGTCAGG GCTCTGGCAG GAATGGCTCT
    AGTTTCCATG GCTGCATCCG AAATCTCTAC ATTAATGACC AACTCCAGGA TCTCACCCAG
    TTTTTGCTGC AGGAGGGTGT TGTGCCCGGA TGCCAGCCAT GCCAGCGGAG CATGTGTGCT
    CATGGCCAGT GCCATGCCAC TGGACAGTCC TCTTTCAGCT GTGAGTGTGA GGCGGGATGG
    ACTGGCCCGC TCTGTGACCA GCAGGTCAAC AACCCATGTG ACGGGAACAA GTGTATCCAT
    GGCTCGTGTA TGGCCATAAA CTCATACTCG TACAGCTGCC GTTGTCTTCC AGGCTTTGCA
    GGTGTGCTGT GTGATGAAGA GGAGCAGCTC AGCCCCTGTC AGTATATAGC ATGCAAATAC
    GGCCGATGTC GTGTGTCTGG ACTGGGCAAA GCCTATTGTG AGTGCAACAG CGGCTACACG
    GGGCAAAGCT GCGACAGAGA GATGGCGTGT CGGGGGGAAC GGGTCCGAGA TCACTATCAG
    ACGCAGCAGG GCTACGCCGC GTGCCAGAGC ACAGAGAAGG TCTCGCGTTT AGAGTGTCGG
    GGCAGCTGTG GGGACGGGAC CTCCTGCTGC GCCCCGCTCC GCAGCAAACG CAGGAAATAC
    ACCTTCCAAT GCACAGACGG GTCCTCTTTT GTACAGGAGG TGGAAAAGGT TGTCAAGTGT
    GGCTGCACAA AATGTCCATC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147295
    Clone ID Related Accession (Same CDS sequence) XM_021475851.1
    Accession Version XM_021475851.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGCG TAGAAGACTA CCGCTCAAAA CTAGGTGGGG ATTGCTTTGC TGATTTGGCC
    TGTCCAGAGA AATGTCGATG TGAAGGAACC ACAGTGGACT GCTCTGGACA AAAGCTCACC
    AAGATCCCAG ACCACATCCC TCAGTATACA GCAGAACTCC GCTTGAATAA TAATGAATTC
    ACAGTGCTGG AGGCTACAGG AATCTTCAAG AAGCTTCCTC AGTTGCGCAA AATCAATCTT
    AGCAACAATA AGATCACAGA TATCGAAGAA GGCACATTTG AAGGGGCCAG TGGAGTAAAT
    GAACTCATAC TGACCAGCAA TCGCTTGGAG GGGGTTCACT ACAGCATGCT GAAAGGCCTC
    GGTGGTCTCC GCACACTGAT GTTGAGGAGT AACAGGATAA GCTGTGTCAA TAACGGCAGT
    TTTACCGGTC TGAGCTCTGT GAGGTTGCTT TCCCTCTATG ATAACCTGAT CACCTCCATG
    TCCCCAGGAG CCTTTGACAC ACTACATTCG CTCTCCACAT TGAACTTGTT GGCCAATCCG
    TTTAACTGTA ACTGCCATCT GGCCTGGCTT GGTGAATGGC TCCGCAAGAA ACGGATTGTC
    ACAGGCAACC CCCGCTGTCA GAGCCCCTAC TTTCTCAAGG AGATCCCTAT TCAAGATGTA
    GCTGTACAAG ACTTTGCCTG TGAAGAAGGT AACGATGAGA ATAGCTGTTC ACCACTGGCT
    CGCTGTCCTG CCGAGTGTTC GTGTTTGGAT ACAGTGGTTC GTTGCAGTAA TAAAGGACTC
    AAGGTTTTGC CTAAGGGCAT TCCTAGAGAC GTTACAGAGC TCTATCTCGA TGGCAACGAG
    TTCACACAAG TCCCTCTGGA ACTATCCAAT TATAAACACC TCACTCTCAT TGATTTGAGT
    AATAACCAGA TCAGCACATT GTCCAACCAC AGCTTCAGTA ACATGTCCGA GCTGCTCACA
    CTAATTTTAA GCTACAACAG ATTAAGGTGT ATTCCTGCAA AAGCGTTCGA CGGCCTCAAG
    TCTCTGCGCT TGTTGTCTCT CCATGGTAAT GATATTGCTG TGATCCCAGA TGGAGCTTTC
    AAAGATCTGT CCTCACTCTC TCACCTGGCT CTGGGCGCAA ACCCCCTGTA CTGTGACTGT
    CACATGCAGT GGCTCTCTGA CTGGGTAAAG AGTGGATACA AGGAGCCTGG CATCGCCCGC
    TGTACCGGTC CTGGAGACAT GACTGACAAA CTGCTGCTCA CTACACCCTC TAAGAAATTC
    ACATGCACAG AGGTGCCGGG GACTTGTGGT TTTCCTGTGC CAGGTCCAGT GGATGTAAGC
    ATTTTGGCTA AATGTAACCC TTGCTTGTCC AATCCCTGCA AGAATGATGG GACCTGCAGT
    AACCATCCTG TAGATTTTTA CAGATGCACC TGCCCGTATG GTTTTAAGGG TCAAGACTGT
    GAGGAACCAA TTCATGCATG CATCAGTAAC CCCTGTCAGA ATGGAGGAAC CTGTCATCTG
    AAGGATGGAG AGGAAAACAC TCACTGGTGT GTGTGTCCAG AAGGATTTGA GGGTGATGAA
    TGTGAGATAA ACATTGACGA CTGTGAGGAT AATGACTGTG AGAATAACTC TACATGCGTG
    GACGGTATCA ACAACTATAC CTGTCTCTGC TCACCTGAAT ACACAGCCGC TAATAATAAT
    GAAGTGGAGA AAGGGGAGCT GTGTGAAGAT AAGCTGGATT TTTGTGCTTC AGAGCTTAAC
    CTGTGTCAGC ATGACTCCAA ATGTATTCTG ACTGCTAAAG GCTTCATGTG TGAATGCACT
    CCAGGTTATA CCGGTGAGCA CTGTGAGGTG GATTTTGATG ATTGTGAAGA TAATAAGTGT
    AAAAACGGGG CTCAGTGTAT TGATGCTGTG AACGGCTACA CGTGTGTGTG TCCAGAAGGC
    TACAGTGGGT TGTTCTGCGA GTTTTCTCCT CCGATGGTCC TGCCAAGAAC AAGTCCATGC
    GATCACTACG ACTGTGCCAA CGGTGCACAG TGTGTTGTGA AGGACACGGA CCCCGTGTGT
    CAGTGTCTGC CAGGTTATGA GGGTGTGCAC TGTGAAAGAC TGGTCAGCGT CAACTTCATC
    AACAGAGAAT CCTTCCTGCA AATCCCCTCC AACCTCATTA CAGAACAGGC CAATATATCT
    CTGCAGATCG CCACAGATGA GGATAATGGA GTGTTGCTGT ACAAAGGAGA TAATGAACAC
    ATTGCTGTGG AGCTTTACAG AGGCCGACTG AGAGTCAGTT ATGACTCTGG ATCATATCCT
    CCATCTGCAA TCTACAGTGT CGAGACAATA AATGATGGCA GCTTTCATGT AGTGGAGCTG
    GTGGCAAAGG ATCAGTCTCT GTCACTGTCC ATTGATGGAG GAAGCCCGAA ATCCATCAAC
    ACTGCCAATA GCCCCAGCCC AGTGCCCAGC CCGGCCCCGC TTTATCTTGG TGGTCTTCCT
    CAGCAGTCTG GATTGGCCTC GTTGCGTCAG GGCTCTGGCA GGAATGGCTC TAGTTTCCAT
    GGCTGCATCC GAAATCTCTA CATTAATGAC CAACTCCAGG ATCTCACCCA GTTTTTGCTG
    CAGGAGGGTG TTGTGCCCGG ATGCCAGCCA TGCCAGCGGA GCATGTGTGC TCATGGCCAG
    TGCCATGCCA CTGGACAGTC CTCTTTCAGC TGTGAGTGTG AGGCGGGATG GACTGGCCCG
    CTCTGTGACC AGCAGGTCAA CAACCCATGT GACGGGAACA AGTGTATCCA TGGCTCGTGT
    ATGGCCATAA ACTCATACTC GTACAGCTGC CGTTGTCTTC CAGGCTTTGC AGGTGTGCTG
    TGTGATGAAG AGGAGCAGCT CAGCCCCTGT CAGTATATAG CATGCAAATA CGGCCGATGT
    CGTGTGTCTG GACTGGGCAA AGCCTATTGT GAGTGCAACA GCGGCTACAC GGGGCAAAGC
    TGCGACAGAG AGATGGCGTG TCGGGGGGAA CGGGTCCGAG ATCACTATCA GACGCAGCAG
    GGCTACGCCG CGTGCCAGAG CACAGAGAAG GTCTCGCGTT TAGAGTGTCG GGGCAGCTGT
    GGGGACGGGA CCTCCTGCTG CGCCCCGCTC CGCAGCAAAC GCAGGAAATA CACCTTCCAA
    TGCACAGACG GGTCCTCTTT TGTACAGGAG GTGGAAAAGG TTGTCAAGTG TGGCTGCACA
    AAATGTCCAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331526.1
    CDS827..5461
    Translation

    Target ORF information:

    RefSeq Version XM_021475851.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475851.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGCG TAGAAGACTA CCGCTCAAAA CTAGGTGGGG ATTGCTTTGC TGATTTGGCC
    TGTCCAGAGA AATGTCGATG TGAAGGAACC ACAGTGGACT GCTCTGGACA AAAGCTCACC
    AAGATCCCAG ACCACATCCC TCAGTATACA GCAGAACTCC GCTTGAATAA TAATGAATTC
    ACAGTGCTGG AGGCTACAGG AATCTTCAAG AAGCTTCCTC AGTTGCGCAA AATCAATCTT
    AGCAACAATA AGATCACAGA TATCGAAGAA GGCACATTTG AAGGGGCCAG TGGAGTAAAT
    GAACTCATAC TGACCAGCAA TCGCTTGGAG GGGGTTCACT ACAGCATGCT GAAAGGCCTC
    GGTGGTCTCC GCACACTGAT GTTGAGGAGT AACAGGATAA GCTGTGTCAA TAACGGCAGT
    TTTACCGGTC TGAGCTCTGT GAGGTTGCTT TCCCTCTATG ATAACCTGAT CACCTCCATG
    TCCCCAGGAG CCTTTGACAC ACTACATTCG CTCTCCACAT TGAACTTGTT GGCCAATCCG
    TTTAACTGTA ACTGCCATCT GGCCTGGCTT GGTGAATGGC TCCGCAAGAA ACGGATTGTC
    ACAGGCAACC CCCGCTGTCA GAGCCCCTAC TTTCTCAAGG AGATCCCTAT TCAAGATGTA
    GCTGTACAAG ACTTTGCCTG TGAAGAAGGT AACGATGAGA ATAGCTGTTC ACCACTGGCT
    CGCTGTCCTG CCGAGTGTTC GTGTTTGGAT ACAGTGGTTC GTTGCAGTAA TAAAGGACTC
    AAGGTTTTGC CTAAGGGCAT TCCTAGAGAC GTTACAGAGC TCTATCTCGA TGGCAACGAG
    TTCACACAAG TCCCTCTGGA ACTATCCAAT TATAAACACC TCACTCTCAT TGATTTGAGT
    AATAACCAGA TCAGCACATT GTCCAACCAC AGCTTCAGTA ACATGTCCGA GCTGCTCACA
    CTAATTTTAA GCTACAACAG ATTAAGGTGT ATTCCTGCAA AAGCGTTCGA CGGCCTCAAG
    TCTCTGCGCT TGTTGTCTCT CCATGGTAAT GATATTGCTG TGATCCCAGA TGGAGCTTTC
    AAAGATCTGT CCTCACTCTC TCACCTGGCT CTGGGCGCAA ACCCCCTGTA CTGTGACTGT
    CACATGCAGT GGCTCTCTGA CTGGGTAAAG AGTGGATACA AGGAGCCTGG CATCGCCCGC
    TGTACCGGTC CTGGAGACAT GACTGACAAA CTGCTGCTCA CTACACCCTC TAAGAAATTC
    ACATGCACAG AGGTGCCGGG GACTTGTGGT TTTCCTGTGC CAGGTCCAGT GGATGTAAGC
    ATTTTGGCTA AATGTAACCC TTGCTTGTCC AATCCCTGCA AGAATGATGG GACCTGCAGT
    AACCATCCTG TAGATTTTTA CAGATGCACC TGCCCGTATG GTTTTAAGGG TCAAGACTGT
    GAGGAACCAA TTCATGCATG CATCAGTAAC CCCTGTCAGA ATGGAGGAAC CTGTCATCTG
    AAGGATGGAG AGGAAAACAC TCACTGGTGT GTGTGTCCAG AAGGATTTGA GGGTGATGAA
    TGTGAGATAA ACATTGACGA CTGTGAGGAT AATGACTGTG AGAATAACTC TACATGCGTG
    GACGGTATCA ACAACTATAC CTGTCTCTGC TCACCTGAAT ACACAGCCGC TAATAATAAT
    GAAGTGGAGA AAGGGGAGCT GTGTGAAGAT AAGCTGGATT TTTGTGCTTC AGAGCTTAAC
    CTGTGTCAGC ATGACTCCAA ATGTATTCTG ACTGCTAAAG GCTTCATGTG TGAATGCACT
    CCAGGTTATA CCGGTGAGCA CTGTGAGGTG GATTTTGATG ATTGTGAAGA TAATAAGTGT
    AAAAACGGGG CTCAGTGTAT TGATGCTGTG AACGGCTACA CGTGTGTGTG TCCAGAAGGC
    TACAGTGGGT TGTTCTGCGA GTTTTCTCCT CCGATGGTCC TGCCAAGAAC AAGTCCATGC
    GATCACTACG ACTGTGCCAA CGGTGCACAG TGTGTTGTGA AGGACACGGA CCCCGTGTGT
    CAGTGTCTGC CAGGTTATGA GGGTGTGCAC TGTGAAAGAC TGGTCAGCGT CAACTTCATC
    AACAGAGAAT CCTTCCTGCA AATCCCCTCC AACCTCATTA CAGAACAGGC CAATATATCT
    CTGCAGATCG CCACAGATGA GGATAATGGA GTGTTGCTGT ACAAAGGAGA TAATGAACAC
    ATTGCTGTGG AGCTTTACAG AGGCCGACTG AGAGTCAGTT ATGACTCTGG ATCATATCCT
    CCATCTGCAA TCTACAGTGT CGAGACAATA AATGATGGCA GCTTTCATGT AGTGGAGCTG
    GTGGCAAAGG ATCAGTCTCT GTCACTGTCC ATTGATGGAG GAAGCCCGAA ATCCATCAAC
    ACTGCCAATA GCCCCAGCCC AGTGCCCAGC CCGGCCCCGC TTTATCTTGG TGGTCTTCCT
    CAGCAGTCTG GATTGGCCTC GTTGCGTCAG GGCTCTGGCA GGAATGGCTC TAGTTTCCAT
    GGCTGCATCC GAAATCTCTA CATTAATGAC CAACTCCAGG ATCTCACCCA GTTTTTGCTG
    CAGGAGGGTG TTGTGCCCGG ATGCCAGCCA TGCCAGCGGA GCATGTGTGC TCATGGCCAG
    TGCCATGCCA CTGGACAGTC CTCTTTCAGC TGTGAGTGTG AGGCGGGATG GACTGGCCCG
    CTCTGTGACC AGCAGGTCAA CAACCCATGT GACGGGAACA AGTGTATCCA TGGCTCGTGT
    ATGGCCATAA ACTCATACTC GTACAGCTGC CGTTGTCTTC CAGGCTTTGC AGGTGTGCTG
    TGTGATGAAG AGGAGCAGCT CAGCCCCTGT CAGTATATAG CATGCAAATA CGGCCGATGT
    CGTGTGTCTG GACTGGGCAA AGCCTATTGT GAGTGCAACA GCGGCTACAC GGGGCAAAGC
    TGCGACAGAG AGATGGCGTG TCGGGGGGAA CGGGTCCGAG ATCACTATCA GACGCAGCAG
    GGCTACGCCG CGTGCCAGAG CACAGAGAAG GTCTCGCGTT TAGAGTGTCG GGGCAGCTGT
    GGGGACGGGA CCTCCTGCTG CGCCCCGCTC CGCAGCAAAC GCAGGAAATA CACCTTCCAA
    TGCACAGACG GGTCCTCTTT TGTACAGGAG GTGGAAAAGG TTGTCAAGTG TGGCTGCACA
    AAATGTCCAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147296
    Clone ID Related Accession (Same CDS sequence) XM_021475852.1
    Accession Version XM_021475852.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGGGATTCA TCATATCAAT
    AAGTGCGTAG AAGACTACCG CTCAAAACTA GGTGGGGATT GCTTTGCTGA TTTGGCCTGT
    CCAGAGAAAT GTCGATGTGA AGGAACCACA GTGGACTGCT CTGGACAAAA GCTCACCAAG
    ATCCCAGACC ACATCCCTCA GTATACAGCA GAACTCCGCT TGAATAATAA TGAATTCACA
    GTGCTGGAGG CTACAGGAAT CTTCAAGAAG CTTCCTCAGT TGCGCAAAAT CAATCTTAGC
    AACAATAAGA TCACAGATAT CGAAGAAGGC ACATTTGAAG GGGCCAGTGG AGTAAATGAA
    CTCATACTGA CCAGCAATCG CTTGGAGGGG GTTCACTACA GCATGCTGAA AGGCCTCGGT
    GGTCTCCGCA CACTGATGTT GAGGAGTAAC AGGATAAGCT GTGTCAATAA CGGCAGTTTT
    ACCGGTCTGA GCTCTGTGAG GTTGCTTTCC CTCTATGATA ACCTGATCAC CTCCATGTCC
    CCAGGAGCCT TTGACACACT ACATTCGCTC TCCACATTGA ACTTGTTGGC CAATCCGTTT
    AACTGTAACT GCCATCTGGC CTGGCTTGGT GAATGGCTCC GCAAGAAACG GATTGTCACA
    GGCAACCCCC GCTGTCAGAG CCCCTACTTT CTCAAGGAGA TCCCTATTCA AGATGTAGCT
    GTACAAGACT TTGCCTGTGA AGAAGGTAAC GATGAGAATA GCTGTTCACC ACTGGCTCGC
    TGTCCTGCCG AGTGTTCGTG TTTGGATACA GTGGTTCGTT GCAGTAATAA AGGACTCAAG
    GTTTTGCCTA AGGGCATTCC TAGAGACGTT ACAGAGCTCT ATCTCGATGG CAACGAGTTC
    ACACAAGTCC CTCTGGAACT ATCCAATTAT AAACACCTCA CTCTCATTGA TTTGAGTAAT
    AACCAGATCA GCACATTGTC CAACCACAGC TTCAGTAACA TGTCCGAGCT GCTCACACTA
    ATTTTAAGCT ACAACAGATT AAGGTGTATT CCTGCAAAAG CGTTCGACGG CCTCAAGTCT
    CTGCGCTTGT TGTCTCTCCA TGGTAATGAT ATTGCTGTGA TCCCAGATGG AGCTTTCAAA
    GATCTGTCCT CACTCTCTCA CCTGGCTCTG GGCGCAAACC CCCTGTACTG TGACTGTCAC
    ATGCAGTGGC TCTCTGACTG GGTAAAGAGT GGATACAAGG AGCCTGGCAT CGCCCGCTGT
    ACCGGTCCTG GAGACATGAC TGACAAACTG CTGCTCACTA CACCCTCTAA GAAATTCACA
    TGCACAGAGG TGCCGGGGAC TTGTGGTTTT CCTGTGCCAG GTCCAGTGGA TGTAAGCATT
    TTGGCTAAAT GTAACCCTTG CTTGTCCAAT CCCTGCAAGA ATGATGGGAC CTGCAGTAAC
    CATCCTGTAG ATTTTTACAG ATGCACCTGC CCGTATGGTT TTAAGGGTCA AGACTGTGAG
    GAACCAATTC ATGCATGCAT CAGTAACCCC TGTCAGAATG GAGGAACCTG TCATCTGAAG
    GATGGAGAGG AAAACACTCA CTGGTGTGTG TGTCCAGAAG GATTTGAGGG TGATGAATGT
    GAGATAAACA TTGACGACTG TGAGGATAAT GACTGTGAGA ATAACTCTAC ATGCGTGGAC
    GGTATCAACA ACTATACCTG TCTCTGCTCA CCTGAATACA CAGCCGCTAA TAATAATGAA
    GTGGAGAAAG GGGAGCTGTG TGAAGATAAG CTGGATTTTT GTGCTTCAGA GCTTAACCTG
    TGTCAGCATG ACTCCAAATG TATTCTGACT GCTAAAGGCT TCATGTGTGA ATGCACTCCA
    GGTTATACCG GTGAGCACTG TGAGGTGGAT TTTGATGATT GTGAAGATAA TAAGTGTAAA
    AACGGGGCTC AGTGTATTGA TGCTGTGAAC GGCTACACGT GTGTGTGTCC AGAAGGCTAC
    AGTGGGTTGT TCTGCGAGTT TTCTCCTCCG ATGGTCCTGC CAAGAACAAG TCCATGCGAT
    CACTACGACT GTGCCAACGG TGCACAGTGT GTTGTGAAGG ACACGGACCC CGTGTGTCAG
    TGTCTGCCAG GTTATGAGGG TGTGCACTGT GAAAGACTGG TCAGCGTCAA CTTCATCAAC
    AGAGAATCCT TCCTGCAAAT CCCCTCCAAC CTCATTACAG AACAGGCCAA TATATCTCTG
    CAGATCGCCA CAGATGAGGA TAATGGAGTG TTGCTGTACA AAGGAGATAA TGAACACATT
    GCTGTGGAGC TTTACAGAGG CCGACTGAGA GTCAGTTATG ACTCTGGATC ATATCCTCCA
    TCTGCAATCT ACAGTGTCGA GACAATAAAT GATGGCAGCT TTCATGTAGT GGAGCTGGTG
    GCAAAGGATC AGTCTCTGTC ACTGTCCATT GATGGAGGAA GCCCGAAATC CATCAACACT
    GCCAATAGCC CCAGCCCAGT GCCCAGCCCG GCCCCGCTTT ATCTTGGTGG TCTTCCTCAG
    CAGTCTGGAT TGGCCTCGTT GCGTCAGGGC TCTGGCAGGA ATGGCTCTAG TTTCCATGGC
    TGCATCCGAA ATCTCTACAT TAATGACCAA CTCCAGGATC TCACCCAGTT TTTGCTGCAG
    GAGGGTGTTG TGCCCGGATG CCAGCCATGC CAGCGGAGCA TGTGTGCTCA TGGCCAGTGC
    CATGCCACTG GACAGTCCTC TTTCAGCTGT GAGTGTGAGG CGGGATGGAC TGGCCCGCTC
    TGTGACCAGC AGGTCAACAA CCCATGTGAC GGGAACAAGT GTATCCATGG CTCGTGTATG
    GCCATAAACT CATACTCGTA CAGCTGCCGT TGTCTTCCAG GCTTTGCAGG TGTGCTGTGT
    GATGAAGAGG AGCAGCTCAG CCCCTGTCAG TATATAGCAT GCAAATACGG CCGATGTCGT
    GTGTCTGGAC TGGGCAAAGC CTATTGTGAG TGCAACAGCG GCTACACGGG GCAAAGCTGC
    GACAGAGAGA TGGCGTGTCG GGGGGAACGG GTCCGAGATC ACTATCAGAC GCAGCAGGGC
    TACGCCGCGT GCCAGAGCAC AGAGAAGGTC TCGCGTTTAG AGTGTCGGGG CAGCTGTGGG
    GACGGGACCT CCTGCTGCGC CCCGCTCCGC AGCAAACGCA GGAAATACAC CTTCCAATGC
    ACAGACGGGT CCTCTTTTGT ACAGGAGGTG GAAAAGGTTG TCAAGTGTGG CTGCACAAAA
    TGTCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331527.1
    CDS827..5458
    Translation

    Target ORF information:

    RefSeq Version XM_021475852.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475852.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGGGATTCA TCATATCAAT
    AAGTGCGTAG AAGACTACCG CTCAAAACTA GGTGGGGATT GCTTTGCTGA TTTGGCCTGT
    CCAGAGAAAT GTCGATGTGA AGGAACCACA GTGGACTGCT CTGGACAAAA GCTCACCAAG
    ATCCCAGACC ACATCCCTCA GTATACAGCA GAACTCCGCT TGAATAATAA TGAATTCACA
    GTGCTGGAGG CTACAGGAAT CTTCAAGAAG CTTCCTCAGT TGCGCAAAAT CAATCTTAGC
    AACAATAAGA TCACAGATAT CGAAGAAGGC ACATTTGAAG GGGCCAGTGG AGTAAATGAA
    CTCATACTGA CCAGCAATCG CTTGGAGGGG GTTCACTACA GCATGCTGAA AGGCCTCGGT
    GGTCTCCGCA CACTGATGTT GAGGAGTAAC AGGATAAGCT GTGTCAATAA CGGCAGTTTT
    ACCGGTCTGA GCTCTGTGAG GTTGCTTTCC CTCTATGATA ACCTGATCAC CTCCATGTCC
    CCAGGAGCCT TTGACACACT ACATTCGCTC TCCACATTGA ACTTGTTGGC CAATCCGTTT
    AACTGTAACT GCCATCTGGC CTGGCTTGGT GAATGGCTCC GCAAGAAACG GATTGTCACA
    GGCAACCCCC GCTGTCAGAG CCCCTACTTT CTCAAGGAGA TCCCTATTCA AGATGTAGCT
    GTACAAGACT TTGCCTGTGA AGAAGGTAAC GATGAGAATA GCTGTTCACC ACTGGCTCGC
    TGTCCTGCCG AGTGTTCGTG TTTGGATACA GTGGTTCGTT GCAGTAATAA AGGACTCAAG
    GTTTTGCCTA AGGGCATTCC TAGAGACGTT ACAGAGCTCT ATCTCGATGG CAACGAGTTC
    ACACAAGTCC CTCTGGAACT ATCCAATTAT AAACACCTCA CTCTCATTGA TTTGAGTAAT
    AACCAGATCA GCACATTGTC CAACCACAGC TTCAGTAACA TGTCCGAGCT GCTCACACTA
    ATTTTAAGCT ACAACAGATT AAGGTGTATT CCTGCAAAAG CGTTCGACGG CCTCAAGTCT
    CTGCGCTTGT TGTCTCTCCA TGGTAATGAT ATTGCTGTGA TCCCAGATGG AGCTTTCAAA
    GATCTGTCCT CACTCTCTCA CCTGGCTCTG GGCGCAAACC CCCTGTACTG TGACTGTCAC
    ATGCAGTGGC TCTCTGACTG GGTAAAGAGT GGATACAAGG AGCCTGGCAT CGCCCGCTGT
    ACCGGTCCTG GAGACATGAC TGACAAACTG CTGCTCACTA CACCCTCTAA GAAATTCACA
    TGCACAGAGG TGCCGGGGAC TTGTGGTTTT CCTGTGCCAG GTCCAGTGGA TGTAAGCATT
    TTGGCTAAAT GTAACCCTTG CTTGTCCAAT CCCTGCAAGA ATGATGGGAC CTGCAGTAAC
    CATCCTGTAG ATTTTTACAG ATGCACCTGC CCGTATGGTT TTAAGGGTCA AGACTGTGAG
    GAACCAATTC ATGCATGCAT CAGTAACCCC TGTCAGAATG GAGGAACCTG TCATCTGAAG
    GATGGAGAGG AAAACACTCA CTGGTGTGTG TGTCCAGAAG GATTTGAGGG TGATGAATGT
    GAGATAAACA TTGACGACTG TGAGGATAAT GACTGTGAGA ATAACTCTAC ATGCGTGGAC
    GGTATCAACA ACTATACCTG TCTCTGCTCA CCTGAATACA CAGCCGCTAA TAATAATGAA
    GTGGAGAAAG GGGAGCTGTG TGAAGATAAG CTGGATTTTT GTGCTTCAGA GCTTAACCTG
    TGTCAGCATG ACTCCAAATG TATTCTGACT GCTAAAGGCT TCATGTGTGA ATGCACTCCA
    GGTTATACCG GTGAGCACTG TGAGGTGGAT TTTGATGATT GTGAAGATAA TAAGTGTAAA
    AACGGGGCTC AGTGTATTGA TGCTGTGAAC GGCTACACGT GTGTGTGTCC AGAAGGCTAC
    AGTGGGTTGT TCTGCGAGTT TTCTCCTCCG ATGGTCCTGC CAAGAACAAG TCCATGCGAT
    CACTACGACT GTGCCAACGG TGCACAGTGT GTTGTGAAGG ACACGGACCC CGTGTGTCAG
    TGTCTGCCAG GTTATGAGGG TGTGCACTGT GAAAGACTGG TCAGCGTCAA CTTCATCAAC
    AGAGAATCCT TCCTGCAAAT CCCCTCCAAC CTCATTACAG AACAGGCCAA TATATCTCTG
    CAGATCGCCA CAGATGAGGA TAATGGAGTG TTGCTGTACA AAGGAGATAA TGAACACATT
    GCTGTGGAGC TTTACAGAGG CCGACTGAGA GTCAGTTATG ACTCTGGATC ATATCCTCCA
    TCTGCAATCT ACAGTGTCGA GACAATAAAT GATGGCAGCT TTCATGTAGT GGAGCTGGTG
    GCAAAGGATC AGTCTCTGTC ACTGTCCATT GATGGAGGAA GCCCGAAATC CATCAACACT
    GCCAATAGCC CCAGCCCAGT GCCCAGCCCG GCCCCGCTTT ATCTTGGTGG TCTTCCTCAG
    CAGTCTGGAT TGGCCTCGTT GCGTCAGGGC TCTGGCAGGA ATGGCTCTAG TTTCCATGGC
    TGCATCCGAA ATCTCTACAT TAATGACCAA CTCCAGGATC TCACCCAGTT TTTGCTGCAG
    GAGGGTGTTG TGCCCGGATG CCAGCCATGC CAGCGGAGCA TGTGTGCTCA TGGCCAGTGC
    CATGCCACTG GACAGTCCTC TTTCAGCTGT GAGTGTGAGG CGGGATGGAC TGGCCCGCTC
    TGTGACCAGC AGGTCAACAA CCCATGTGAC GGGAACAAGT GTATCCATGG CTCGTGTATG
    GCCATAAACT CATACTCGTA CAGCTGCCGT TGTCTTCCAG GCTTTGCAGG TGTGCTGTGT
    GATGAAGAGG AGCAGCTCAG CCCCTGTCAG TATATAGCAT GCAAATACGG CCGATGTCGT
    GTGTCTGGAC TGGGCAAAGC CTATTGTGAG TGCAACAGCG GCTACACGGG GCAAAGCTGC
    GACAGAGAGA TGGCGTGTCG GGGGGAACGG GTCCGAGATC ACTATCAGAC GCAGCAGGGC
    TACGCCGCGT GCCAGAGCAC AGAGAAGGTC TCGCGTTTAG AGTGTCGGGG CAGCTGTGGG
    GACGGGACCT CCTGCTGCGC CCCGCTCCGC AGCAAACGCA GGAAATACAC CTTCCAATGC
    ACAGACGGGT CCTCTTTTGT ACAGGAGGTG GAAAAGGTTG TCAAGTGTGG CTGCACAAAA
    TGTCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147297
    Clone ID Related Accession (Same CDS sequence) XM_021475853.1
    Accession Version XM_021475853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGATTTG AGGGTGATGA ATGTGAGATA AACATTGACG ACTGTGAGGA TAATGACTGT
    GAGAATAACT CTACATGCGT GGACGGTATC AACAACTATA CCTGTCTCTG CTCACCTGAA
    TACACAGGGG AGCTGTGTGA AGATAAGCTG GATTTTTGTG CTTCAGAGCT TAACCTGTGT
    CAGCATGACT CCAAATGTAT TCTGACTGCT AAAGGCTTCA TGTGTGAATG CACTCCAGGT
    TATACCGGTG AGCACTGTGA GGTGGATTTT GATGATTGTG AAGATAATAA GTGTAAAAAC
    GGGGCTCAGT GTATTGATGC TGTGAACGGC TACACGTGTG TGTGTCCAGA AGGCTACAGT
    GGGTTGTTCT GCGAGTTTTC TCCTCCGATG GTCCTGCCAA GAACAAGTCC ATGCGATCAC
    TACGACTGTG CCAACGGTGC ACAGTGTGTT GTGAAGGACA CGGACCCCGT GTGTCAGTGT
    CTGCCAGGTT ATGAGGGTGT GCACTGTGAA AGACTGGTCA GCGTCAACTT CATCAACAGA
    GAATCCTTCC TGCAAATCCC CTCCAACCTC ATTACAGAAC AGGCCAATAT ATCTCTGCAG
    ATCGCCACAG ATGAGGATAA TGGAGTGTTG CTGTACAAAG GAGATAATGA ACACATTGCT
    GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC AGTTATGACT CTGGATCATA TCCTCCATCT
    GCAATCTACA GTGTCGAGAC AATAAATGAT GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA
    AAGGATCAGT CTCTGTCACT GTCCATTGAT GGAGGAAGCC CGAAATCCAT CAACACTGCC
    AATAGCCCCA GCCCAGTGCC CAGCCCGGCC CCGCTTTATC TTGGTGGTCT TCCTCAGCAG
    TCTGGATTGG CCTCGTTGCG TCAGGGCTCT GGCAGGAATG GCTCTAGTTT CCATGGCTGC
    ATCCGAAATC TCTACATTAA TGACCAACTC CAGGATCTCA CCCAGTTTTT GCTGCAGGAG
    GGTGTTGTGC CCGGATGCCA GCCATGCCAG CGGAGCATGT GTGCTCATGG CCAGTGCCAT
    GCCACTGGAC AGTCCTCTTT CAGCTGTGAG TGTGAGGCGG GATGGACTGG CCCGCTCTGT
    GACCAGCAGG TCAACAACCC ATGTGACGGG AACAAGTGTA TCCATGGCTC GTGTATGGCC
    ATAAACTCAT ACTCGTACAG CTGCCGTTGT CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT
    GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT ATAGCATGCA AATACGGCCG ATGTCGTGTG
    TCTGGACTGG GCAAAGCCTA TTGTGAGTGC AACAGCGGCT ACACGGGGCA AAGCTGCGAC
    AGAGAGATGG CGTGTCGGGG GGAACGGGTC CGAGATCACT ATCAGACGCA GCAGGGCTAC
    GCCGCGTGCC AGAGCACAGA GAAGGTCTCG CGTTTAGAGT GTCGGGGCAG CTGTGGGGAC
    GGGACCTCCT GCTGCGCCCC GCTCCGCAGC AAACGCAGGA AATACACCTT CCAATGCACA
    GACGGGTCCT CTTTTGTACA GGAGGTGGAA AAGGTTGTCA AGTGTGGCTG CACAAAATGT
    CCATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331528.1
    CDS827..5455
    Translation

    Target ORF information:

    RefSeq Version XM_021475853.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGATTTG AGGGTGATGA ATGTGAGATA AACATTGACG ACTGTGAGGA TAATGACTGT
    GAGAATAACT CTACATGCGT GGACGGTATC AACAACTATA CCTGTCTCTG CTCACCTGAA
    TACACAGGGG AGCTGTGTGA AGATAAGCTG GATTTTTGTG CTTCAGAGCT TAACCTGTGT
    CAGCATGACT CCAAATGTAT TCTGACTGCT AAAGGCTTCA TGTGTGAATG CACTCCAGGT
    TATACCGGTG AGCACTGTGA GGTGGATTTT GATGATTGTG AAGATAATAA GTGTAAAAAC
    GGGGCTCAGT GTATTGATGC TGTGAACGGC TACACGTGTG TGTGTCCAGA AGGCTACAGT
    GGGTTGTTCT GCGAGTTTTC TCCTCCGATG GTCCTGCCAA GAACAAGTCC ATGCGATCAC
    TACGACTGTG CCAACGGTGC ACAGTGTGTT GTGAAGGACA CGGACCCCGT GTGTCAGTGT
    CTGCCAGGTT ATGAGGGTGT GCACTGTGAA AGACTGGTCA GCGTCAACTT CATCAACAGA
    GAATCCTTCC TGCAAATCCC CTCCAACCTC ATTACAGAAC AGGCCAATAT ATCTCTGCAG
    ATCGCCACAG ATGAGGATAA TGGAGTGTTG CTGTACAAAG GAGATAATGA ACACATTGCT
    GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC AGTTATGACT CTGGATCATA TCCTCCATCT
    GCAATCTACA GTGTCGAGAC AATAAATGAT GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA
    AAGGATCAGT CTCTGTCACT GTCCATTGAT GGAGGAAGCC CGAAATCCAT CAACACTGCC
    AATAGCCCCA GCCCAGTGCC CAGCCCGGCC CCGCTTTATC TTGGTGGTCT TCCTCAGCAG
    TCTGGATTGG CCTCGTTGCG TCAGGGCTCT GGCAGGAATG GCTCTAGTTT CCATGGCTGC
    ATCCGAAATC TCTACATTAA TGACCAACTC CAGGATCTCA CCCAGTTTTT GCTGCAGGAG
    GGTGTTGTGC CCGGATGCCA GCCATGCCAG CGGAGCATGT GTGCTCATGG CCAGTGCCAT
    GCCACTGGAC AGTCCTCTTT CAGCTGTGAG TGTGAGGCGG GATGGACTGG CCCGCTCTGT
    GACCAGCAGG TCAACAACCC ATGTGACGGG AACAAGTGTA TCCATGGCTC GTGTATGGCC
    ATAAACTCAT ACTCGTACAG CTGCCGTTGT CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT
    GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT ATAGCATGCA AATACGGCCG ATGTCGTGTG
    TCTGGACTGG GCAAAGCCTA TTGTGAGTGC AACAGCGGCT ACACGGGGCA AAGCTGCGAC
    AGAGAGATGG CGTGTCGGGG GGAACGGGTC CGAGATCACT ATCAGACGCA GCAGGGCTAC
    GCCGCGTGCC AGAGCACAGA GAAGGTCTCG CGTTTAGAGT GTCGGGGCAG CTGTGGGGAC
    GGGACCTCCT GCTGCGCCCC GCTCCGCAGC AAACGCAGGA AATACACCTT CCAATGCACA
    GACGGGTCCT CTTTTGTACA GGAGGTGGAA AAGGTTGTCA AGTGTGGCTG CACAAAATGT
    CCATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147298
    Clone ID Related Accession (Same CDS sequence) XM_021475854.1
    Accession Version XM_021475854.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4623bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GGTCCAGTGG ATGTAAGCAT TTTGGCTAAA
    TGTAACCCTT GCTTGTCCAA TCCCTGCAAG AATGATGGGA CCTGCAGTAA CCATCCTGTA
    GATTTTTACA GATGCACCTG CCCGTATGGT TTTAAGGGTC AAGACTGTGA GGAACCAATT
    CATGCATGCA TCAGTAACCC CTGTCAGAAT GGAGGAACCT GTCATCTGAA GGATGGAGAG
    GAAAACACTC ACTGGTGTGT GTGTCCAGAA GGATTTGAGG GTGATGAATG TGAGATAAAC
    ATTGACGACT GTGAGGATAA TGACTGTGAG AATAACTCTA CATGCGTGGA CGGTATCAAC
    AACTATACCT GTCTCTGCTC ACCTGAATAC ACAGCCGCTA ATAATAATGA AGTGGAGAAA
    GGGGAGCTGT GTGAAGATAA GCTGGATTTT TGTGCTTCAG AGCTTAACCT GTGTCAGCAT
    GACTCCAAAT GTATTCTGAC TGCTAAAGGC TTCATGTGTG AATGCACTCC AGGTTATACC
    GGTGAGCACT GTGAGGTGGA TTTTGATGAT TGTGAAGATA ATAAGTGTAA AAACGGGGCT
    CAGTGTATTG ATGCTGTGAA CGGCTACACG TGTGTGTGTC CAGAAGGCTA CAGTGGGTTG
    TTCTGCGAGT TTTCTCCTCC GATGGTCCTG CCAAGAACAA GTCCATGCGA TCACTACGAC
    TGTGCCAACG GTGCACAGTG TGTTGTGAAG GACACGGACC CCGTGTGTCA GTGTCTGCCA
    GGTTATGAGG GTGTGCACTG TGAAAGACTG GTCAGCGTCA ACTTCATCAA CAGAGAATCC
    TTCCTGCAAA TCCCCTCCAA CCTCATTACA GAACAGGCCA ATATATCTCT GCAGATCGCC
    ACAGATGAGG ATAATGGAGT GTTGCTGTAC AAAGGAGATA ATGAACACAT TGCTGTGGAG
    CTTTACAGAG GCCGACTGAG AGTCAGTTAT GACTCTGGAT CATATCCTCC ATCTGCAATC
    TACAGTGTCG AGACAATAAA TGATGGCAGC TTTCATGTAG TGGAGCTGGT GGCAAAGGAT
    CAGTCTCTGT CACTGTCCAT TGATGGAGGA AGCCCGAAAT CCATCAACAC TGCCAATAGC
    CCCAGCCCAG TGCCCAGCCC GGCCCCGCTT TATCTTGGTG GTCTTCCTCA GCAGTCTGGA
    TTGGCCTCGT TGCGTCAGGG CTCTGGCAGG AATGGCTCTA GTTTCCATGG CTGCATCCGA
    AATCTCTACA TTAATGACCA ACTCCAGGAT CTCACCCAGT TTTTGCTGCA GGAGGGTGTT
    GTGCCCGGAT GCCAGCCATG CCAGCGGAGC ATGTGTGCTC ATGGCCAGTG CCATGCCACT
    GGACAGTCCT CTTTCAGCTG TGAGTGTGAG GCGGGATGGA CTGGCCCGCT CTGTGACCAG
    CAGGTCAACA ACCCATGTGA CGGGAACAAG TGTATCCATG GCTCGTGTAT GGCCATAAAC
    TCATACTCGT ACAGCTGCCG TTGTCTTCCA GGCTTTGCAG GTGTGCTGTG TGATGAAGAG
    GAGCAGCTCA GCCCCTGTCA GTATATAGCA TGCAAATACG GCCGATGTCG TGTGTCTGGA
    CTGGGCAAAG CCTATTGTGA GTGCAACAGC GGCTACACGG GGCAAAGCTG CGACAGAGAG
    ATGGCGTGTC GGGGGGAACG GGTCCGAGAT CACTATCAGA CGCAGCAGGG CTACGCCGCG
    TGCCAGAGCA CAGAGAAGGT CTCGCGTTTA GAGTGTCGGG GCAGCTGTGG GGACGGGACC
    TCCTGCTGCG CCCCGCTCCG CAGCAAACGC AGGAAATACA CCTTCCAATG CACAGACGGG
    TCCTCTTTTG TACAGGAGGT GGAAAAGGTT GTCAAGTGTG GCTGCACAAA ATGTCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331529.1
    CDS827..5449
    Translation

    Target ORF information:

    RefSeq Version XM_021475854.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475854.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GGTCCAGTGG ATGTAAGCAT TTTGGCTAAA
    TGTAACCCTT GCTTGTCCAA TCCCTGCAAG AATGATGGGA CCTGCAGTAA CCATCCTGTA
    GATTTTTACA GATGCACCTG CCCGTATGGT TTTAAGGGTC AAGACTGTGA GGAACCAATT
    CATGCATGCA TCAGTAACCC CTGTCAGAAT GGAGGAACCT GTCATCTGAA GGATGGAGAG
    GAAAACACTC ACTGGTGTGT GTGTCCAGAA GGATTTGAGG GTGATGAATG TGAGATAAAC
    ATTGACGACT GTGAGGATAA TGACTGTGAG AATAACTCTA CATGCGTGGA CGGTATCAAC
    AACTATACCT GTCTCTGCTC ACCTGAATAC ACAGCCGCTA ATAATAATGA AGTGGAGAAA
    GGGGAGCTGT GTGAAGATAA GCTGGATTTT TGTGCTTCAG AGCTTAACCT GTGTCAGCAT
    GACTCCAAAT GTATTCTGAC TGCTAAAGGC TTCATGTGTG AATGCACTCC AGGTTATACC
    GGTGAGCACT GTGAGGTGGA TTTTGATGAT TGTGAAGATA ATAAGTGTAA AAACGGGGCT
    CAGTGTATTG ATGCTGTGAA CGGCTACACG TGTGTGTGTC CAGAAGGCTA CAGTGGGTTG
    TTCTGCGAGT TTTCTCCTCC GATGGTCCTG CCAAGAACAA GTCCATGCGA TCACTACGAC
    TGTGCCAACG GTGCACAGTG TGTTGTGAAG GACACGGACC CCGTGTGTCA GTGTCTGCCA
    GGTTATGAGG GTGTGCACTG TGAAAGACTG GTCAGCGTCA ACTTCATCAA CAGAGAATCC
    TTCCTGCAAA TCCCCTCCAA CCTCATTACA GAACAGGCCA ATATATCTCT GCAGATCGCC
    ACAGATGAGG ATAATGGAGT GTTGCTGTAC AAAGGAGATA ATGAACACAT TGCTGTGGAG
    CTTTACAGAG GCCGACTGAG AGTCAGTTAT GACTCTGGAT CATATCCTCC ATCTGCAATC
    TACAGTGTCG AGACAATAAA TGATGGCAGC TTTCATGTAG TGGAGCTGGT GGCAAAGGAT
    CAGTCTCTGT CACTGTCCAT TGATGGAGGA AGCCCGAAAT CCATCAACAC TGCCAATAGC
    CCCAGCCCAG TGCCCAGCCC GGCCCCGCTT TATCTTGGTG GTCTTCCTCA GCAGTCTGGA
    TTGGCCTCGT TGCGTCAGGG CTCTGGCAGG AATGGCTCTA GTTTCCATGG CTGCATCCGA
    AATCTCTACA TTAATGACCA ACTCCAGGAT CTCACCCAGT TTTTGCTGCA GGAGGGTGTT
    GTGCCCGGAT GCCAGCCATG CCAGCGGAGC ATGTGTGCTC ATGGCCAGTG CCATGCCACT
    GGACAGTCCT CTTTCAGCTG TGAGTGTGAG GCGGGATGGA CTGGCCCGCT CTGTGACCAG
    CAGGTCAACA ACCCATGTGA CGGGAACAAG TGTATCCATG GCTCGTGTAT GGCCATAAAC
    TCATACTCGT ACAGCTGCCG TTGTCTTCCA GGCTTTGCAG GTGTGCTGTG TGATGAAGAG
    GAGCAGCTCA GCCCCTGTCA GTATATAGCA TGCAAATACG GCCGATGTCG TGTGTCTGGA
    CTGGGCAAAG CCTATTGTGA GTGCAACAGC GGCTACACGG GGCAAAGCTG CGACAGAGAG
    ATGGCGTGTC GGGGGGAACG GGTCCGAGAT CACTATCAGA CGCAGCAGGG CTACGCCGCG
    TGCCAGAGCA CAGAGAAGGT CTCGCGTTTA GAGTGTCGGG GCAGCTGTGG GGACGGGACC
    TCCTGCTGCG CCCCGCTCCG CAGCAAACGC AGGAAATACA CCTTCCAATG CACAGACGGG
    TCCTCTTTTG TACAGGAGGT GGAAAAGGTT GTCAAGTGTG GCTGCACAAA ATGTCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147299
    Clone ID Related Accession (Same CDS sequence) XM_021475855.1
    Accession Version XM_021475855.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGGCGTAGA AGACTACCGC
    TCAAAACTAG GTGGGGATTG CTTTGCTGAT TTGGCCTGTC CAGAGAAATG TCGATGTGAA
    GGAACCACAG TGGACTGCTC TGGACAAAAG CTCACCAAGA TCCCAGACCA CATCCCTCAG
    TATACAGCAG AACTCCGCTT GAATAATAAT GAATTCACAG TGCTGGAGGC TACAGGAATC
    TTCAAGAAGC TTCCTCAGTT GCGCAAAATC AATCTTAGCA ACAATAAGAT CACAGATATC
    GAAGAAGGCA CATTTGAAGG GGCCAGTGGA GTAAATGAAC TCATACTGAC CAGCAATCGC
    TTGGAGGGGG TTCACTACAG CATGCTGAAA GGCCTCGGTG GTCTCCGCAC ACTGATGTTG
    AGGAGTAACA GGATAAGCTG TGTCAATAAC GGCAGTTTTA CCGGTCTGAG CTCTGTGAGG
    TTGCTTTCCC TCTATGATAA CCTGATCACC TCCATGTCCC CAGGAGCCTT TGACACACTA
    CATTCGCTCT CCACATTGAA CTTGTTGGCC AATCCGTTTA ACTGTAACTG CCATCTGGCC
    TGGCTTGGTG AATGGCTCCG CAAGAAACGG ATTGTCACAG GCAACCCCCG CTGTCAGAGC
    CCCTACTTTC TCAAGGAGAT CCCTATTCAA GATGTAGCTG TACAAGACTT TGCCTGTGAA
    GAAGGTAACG ATGAGAATAG CTGTTCACCA CTGGCTCGCT GTCCTGCCGA GTGTTCGTGT
    TTGGATACAG TGGTTCGTTG CAGTAATAAA GGACTCAAGG TTTTGCCTAA GGGCATTCCT
    AGAGACGTTA CAGAGCTCTA TCTCGATGGC AACGAGTTCA CACAAGTCCC TCTGGAACTA
    TCCAATTATA AACACCTCAC TCTCATTGAT TTGAGTAATA ACCAGATCAG CACATTGTCC
    AACCACAGCT TCAGTAACAT GTCCGAGCTG CTCACACTAA TTTTAAGCTA CAACAGATTA
    AGGTGTATTC CTGCAAAAGC GTTCGACGGC CTCAAGTCTC TGCGCTTGTT GTCTCTCCAT
    GGTAATGATA TTGCTGTGAT CCCAGATGGA GCTTTCAAAG ATCTGTCCTC ACTCTCTCAC
    CTGGCTCTGG GCGCAAACCC CCTGTACTGT GACTGTCACA TGCAGTGGCT CTCTGACTGG
    GTAAAGAGTG GATACAAGGA GCCTGGCATC GCCCGCTGTA CCGGTCCTGG AGACATGACT
    GACAAACTGC TGCTCACTAC ACCCTCTAAG AAATTCACAT GCACAGAGGT GCCGGGGACT
    TGTGGTTTTC CTGTGCCAGG TCCAGTGGAT GTAAGCATTT TGGCTAAATG TAACCCTTGC
    TTGTCCAATC CCTGCAAGAA TGATGGGACC TGCAGTAACC ATCCTGTAGA TTTTTACAGA
    TGCACCTGCC CGTATGGTTT TAAGGGTCAA GACTGTGAGG AACCAATTCA TGCATGCATC
    AGTAACCCCT GTCAGAATGG AGGAACCTGT CATCTGAAGG ATGGAGAGGA AAACACTCAC
    TGGTGTGTGT GTCCAGAAGG ATTTGAGGGT GATGAATGTG AGATAAACAT TGACGACTGT
    GAGGATAATG ACTGTGAGAA TAACTCTACA TGCGTGGACG GTATCAACAA CTATACCTGT
    CTCTGCTCAC CTGAATACAC AGCCGCTAAT AATAATGAAG TGGAGAAAGG GGAGCTGTGT
    GAAGATAAGC TGGATTTTTG TGCTTCAGAG CTTAACCTGT GTCAGCATGA CTCCAAATGT
    ATTCTGACTG CTAAAGGCTT CATGTGTGAA TGCACTCCAG GTTATACCGG TGAGCACTGT
    GAGGTGGATT TTGATGATTG TGAAGATAAT AAGTGTAAAA ACGGGGCTCA GTGTATTGAT
    GCTGTGAACG GCTACACGTG TGTGTGTCCA GAAGGCTACA GTGGGTTGTT CTGCGAGTTT
    TCTCCTCCGA TGGTCCTGCC AAGAACAAGT CCATGCGATC ACTACGACTG TGCCAACGGT
    GCACAGTGTG TTGTGAAGGA CACGGACCCC GTGTGTCAGT GTCTGCCAGG TTATGAGGGT
    GTGCACTGTG AAAGACTGGT CAGCGTCAAC TTCATCAACA GAGAATCCTT CCTGCAAATC
    CCCTCCAACC TCATTACAGA ACAGGCCAAT ATATCTCTGC AGATCGCCAC AGATGAGGAT
    AATGGAGTGT TGCTGTACAA AGGAGATAAT GAACACATTG CTGTGGAGCT TTACAGAGGC
    CGACTGAGAG TCAGTTATGA CTCTGGATCA TATCCTCCAT CTGCAATCTA CAGTGTCGAG
    ACAATAAATG ATGGCAGCTT TCATGTAGTG GAGCTGGTGG CAAAGGATCA GTCTCTGTCA
    CTGTCCATTG ATGGAGGAAG CCCGAAATCC ATCAACACTG CCAATAGCCC CAGCCCAGTG
    CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT CTTCCTCAGC AGTCTGGATT GGCCTCGTTG
    CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT TTCCATGGCT GCATCCGAAA TCTCTACATT
    AATGACCAAC TCCAGGATCT CACCCAGTTT TTGCTGCAGG AGGGTGTTGT GCCCGGATGC
    CAGCCATGCC AGCGGAGCAT GTGTGCTCAT GGCCAGTGCC ATGCCACTGG ACAGTCCTCT
    TTCAGCTGTG AGTGTGAGGC GGGATGGACT GGCCCGCTCT GTGACCAGCA GGTCAACAAC
    CCATGTGACG GGAACAAGTG TATCCATGGC TCGTGTATGG CCATAAACTC ATACTCGTAC
    AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC
    CCCTGTCAGT ATATAGCATG CAAATACGGC CGATGTCGTG TGTCTGGACT GGGCAAAGCC
    TATTGTGAGT GCAACAGCGG CTACACGGGG CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG
    GGGGAACGGG TCCGAGATCA CTATCAGACG CAGCAGGGCT ACGCCGCGTG CCAGAGCACA
    GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC AGCTGTGGGG ACGGGACCTC CTGCTGCGCC
    CCGCTCCGCA GCAAACGCAG GAAATACACC TTCCAATGCA CAGACGGGTC CTCTTTTGTA
    CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331530.1
    CDS827..5437
    Translation

    Target ORF information:

    RefSeq Version XM_021475855.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475855.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGGCGTAGA AGACTACCGC
    TCAAAACTAG GTGGGGATTG CTTTGCTGAT TTGGCCTGTC CAGAGAAATG TCGATGTGAA
    GGAACCACAG TGGACTGCTC TGGACAAAAG CTCACCAAGA TCCCAGACCA CATCCCTCAG
    TATACAGCAG AACTCCGCTT GAATAATAAT GAATTCACAG TGCTGGAGGC TACAGGAATC
    TTCAAGAAGC TTCCTCAGTT GCGCAAAATC AATCTTAGCA ACAATAAGAT CACAGATATC
    GAAGAAGGCA CATTTGAAGG GGCCAGTGGA GTAAATGAAC TCATACTGAC CAGCAATCGC
    TTGGAGGGGG TTCACTACAG CATGCTGAAA GGCCTCGGTG GTCTCCGCAC ACTGATGTTG
    AGGAGTAACA GGATAAGCTG TGTCAATAAC GGCAGTTTTA CCGGTCTGAG CTCTGTGAGG
    TTGCTTTCCC TCTATGATAA CCTGATCACC TCCATGTCCC CAGGAGCCTT TGACACACTA
    CATTCGCTCT CCACATTGAA CTTGTTGGCC AATCCGTTTA ACTGTAACTG CCATCTGGCC
    TGGCTTGGTG AATGGCTCCG CAAGAAACGG ATTGTCACAG GCAACCCCCG CTGTCAGAGC
    CCCTACTTTC TCAAGGAGAT CCCTATTCAA GATGTAGCTG TACAAGACTT TGCCTGTGAA
    GAAGGTAACG ATGAGAATAG CTGTTCACCA CTGGCTCGCT GTCCTGCCGA GTGTTCGTGT
    TTGGATACAG TGGTTCGTTG CAGTAATAAA GGACTCAAGG TTTTGCCTAA GGGCATTCCT
    AGAGACGTTA CAGAGCTCTA TCTCGATGGC AACGAGTTCA CACAAGTCCC TCTGGAACTA
    TCCAATTATA AACACCTCAC TCTCATTGAT TTGAGTAATA ACCAGATCAG CACATTGTCC
    AACCACAGCT TCAGTAACAT GTCCGAGCTG CTCACACTAA TTTTAAGCTA CAACAGATTA
    AGGTGTATTC CTGCAAAAGC GTTCGACGGC CTCAAGTCTC TGCGCTTGTT GTCTCTCCAT
    GGTAATGATA TTGCTGTGAT CCCAGATGGA GCTTTCAAAG ATCTGTCCTC ACTCTCTCAC
    CTGGCTCTGG GCGCAAACCC CCTGTACTGT GACTGTCACA TGCAGTGGCT CTCTGACTGG
    GTAAAGAGTG GATACAAGGA GCCTGGCATC GCCCGCTGTA CCGGTCCTGG AGACATGACT
    GACAAACTGC TGCTCACTAC ACCCTCTAAG AAATTCACAT GCACAGAGGT GCCGGGGACT
    TGTGGTTTTC CTGTGCCAGG TCCAGTGGAT GTAAGCATTT TGGCTAAATG TAACCCTTGC
    TTGTCCAATC CCTGCAAGAA TGATGGGACC TGCAGTAACC ATCCTGTAGA TTTTTACAGA
    TGCACCTGCC CGTATGGTTT TAAGGGTCAA GACTGTGAGG AACCAATTCA TGCATGCATC
    AGTAACCCCT GTCAGAATGG AGGAACCTGT CATCTGAAGG ATGGAGAGGA AAACACTCAC
    TGGTGTGTGT GTCCAGAAGG ATTTGAGGGT GATGAATGTG AGATAAACAT TGACGACTGT
    GAGGATAATG ACTGTGAGAA TAACTCTACA TGCGTGGACG GTATCAACAA CTATACCTGT
    CTCTGCTCAC CTGAATACAC AGCCGCTAAT AATAATGAAG TGGAGAAAGG GGAGCTGTGT
    GAAGATAAGC TGGATTTTTG TGCTTCAGAG CTTAACCTGT GTCAGCATGA CTCCAAATGT
    ATTCTGACTG CTAAAGGCTT CATGTGTGAA TGCACTCCAG GTTATACCGG TGAGCACTGT
    GAGGTGGATT TTGATGATTG TGAAGATAAT AAGTGTAAAA ACGGGGCTCA GTGTATTGAT
    GCTGTGAACG GCTACACGTG TGTGTGTCCA GAAGGCTACA GTGGGTTGTT CTGCGAGTTT
    TCTCCTCCGA TGGTCCTGCC AAGAACAAGT CCATGCGATC ACTACGACTG TGCCAACGGT
    GCACAGTGTG TTGTGAAGGA CACGGACCCC GTGTGTCAGT GTCTGCCAGG TTATGAGGGT
    GTGCACTGTG AAAGACTGGT CAGCGTCAAC TTCATCAACA GAGAATCCTT CCTGCAAATC
    CCCTCCAACC TCATTACAGA ACAGGCCAAT ATATCTCTGC AGATCGCCAC AGATGAGGAT
    AATGGAGTGT TGCTGTACAA AGGAGATAAT GAACACATTG CTGTGGAGCT TTACAGAGGC
    CGACTGAGAG TCAGTTATGA CTCTGGATCA TATCCTCCAT CTGCAATCTA CAGTGTCGAG
    ACAATAAATG ATGGCAGCTT TCATGTAGTG GAGCTGGTGG CAAAGGATCA GTCTCTGTCA
    CTGTCCATTG ATGGAGGAAG CCCGAAATCC ATCAACACTG CCAATAGCCC CAGCCCAGTG
    CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT CTTCCTCAGC AGTCTGGATT GGCCTCGTTG
    CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT TTCCATGGCT GCATCCGAAA TCTCTACATT
    AATGACCAAC TCCAGGATCT CACCCAGTTT TTGCTGCAGG AGGGTGTTGT GCCCGGATGC
    CAGCCATGCC AGCGGAGCAT GTGTGCTCAT GGCCAGTGCC ATGCCACTGG ACAGTCCTCT
    TTCAGCTGTG AGTGTGAGGC GGGATGGACT GGCCCGCTCT GTGACCAGCA GGTCAACAAC
    CCATGTGACG GGAACAAGTG TATCCATGGC TCGTGTATGG CCATAAACTC ATACTCGTAC
    AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC
    CCCTGTCAGT ATATAGCATG CAAATACGGC CGATGTCGTG TGTCTGGACT GGGCAAAGCC
    TATTGTGAGT GCAACAGCGG CTACACGGGG CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG
    GGGGAACGGG TCCGAGATCA CTATCAGACG CAGCAGGGCT ACGCCGCGTG CCAGAGCACA
    GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC AGCTGTGGGG ACGGGACCTC CTGCTGCGCC
    CCGCTCCGCA GCAAACGCAG GAAATACACC TTCCAATGCA CAGACGGGTC CTCTTTTGTA
    CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147300
    Clone ID Related Accession (Same CDS sequence) XM_021475856.1
    Accession Version XM_021475856.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGCG TAGAAGACTA CCGCTCAAAA CTAGGTGGGG ATTGCTTTGC TGATTTGGCC
    TGTCCAGAGA AATGTCGATG TGAAGGAACC ACAGTGGACT GCTCTGGACA AAAGCTCACC
    AAGATCCCAG ACCACATCCC TCAGTATACA GCAGAACTCC GCTTGAATAA TAATGAATTC
    ACAGTGCTGG AGGCTACAGG AATCTTCAAG AAGCTTCCTC AGTTGCGCAA AATCAATCTT
    AGCAACAATA AGATCACAGA TATCGAAGAA GGCACATTTG AAGGGGCCAG TGGAGTAAAT
    GAACTCATAC TGACCAGCAA TCGCTTGGAG GGGGTTCACT ACAGCATGCT GAAAGGCCTC
    GGTGGTCTCC GCACACTGAT GTTGAGGAGT AACAGGATAA GCTGTGTCAA TAACGGCAGT
    TTTACCGGTC TGAGCTCTGT GAGGTTGCTT TCCCTCTATG ATAACCTGAT CACCTCCATG
    TCCCCAGGAG CCTTTGACAC ACTACATTCG CTCTCCACAT TGAACTTGTT GGCCAATCCG
    TTTAACTGTA ACTGCCATCT GGCCTGGCTT GGTGAATGGC TCCGCAAGAA ACGGATTGTC
    ACAGGCAACC CCCGCTGTCA GAGCCCCTAC TTTCTCAAGG AGATCCCTAT TCAAGATGTA
    GCTGTACAAG ACTTTGCCTG TGAAGAAGGT AACGATGAGA ATAGCTGTTC ACCACTGGCT
    CGCTGTCCTG CCGAGTGTTC GTGTTTGGAT ACAGTGGTTC GTTGCAGTAA TAAAGGACTC
    AAGGTTTTGC CTAAGGGCAT TCCTAGAGAC GTTACAGAGC TCTATCTCGA TGGCAACGAG
    TTCACACAAG TCCCTCTGGA ACTATCCAAT TATAAACACC TCACTCTCAT TGATTTGAGT
    AATAACCAGA TCAGCACATT GTCCAACCAC AGCTTCAGTA ACATGTCCGA GCTGCTCACA
    CTAATTTTAA GCTACAACAG ATTAAGGTGT ATTCCTGCAA AAGCGTTCGA CGGCCTCAAG
    TCTCTGCGCT TGTTGTCTCT CCATGGTAAT GATATTGCTG TGATCCCAGA TGGAGCTTTC
    AAAGATCTGT CCTCACTCTC TCACCTGGCT CTGGGCGCAA ACCCCCTGTA CTGTGACTGT
    CACATGCAGT GGCTCTCTGA CTGGGTAAAG AGTGGATACA AGGAGCCTGG CATCGCCCGC
    TGTACCGGTC CTGGAGACAT GACTGACAAA CTGCTGCTCA CTACACCCTC TAAGAAATTC
    ACATGCACAG GTCCAGTGGA TGTAAGCATT TTGGCTAAAT GTAACCCTTG CTTGTCCAAT
    CCCTGCAAGA ATGATGGGAC CTGCAGTAAC CATCCTGTAG ATTTTTACAG ATGCACCTGC
    CCGTATGGTT TTAAGGGTCA AGACTGTGAG GAACCAATTC ATGCATGCAT CAGTAACCCC
    TGTCAGAATG GAGGAACCTG TCATCTGAAG GATGGAGAGG AAAACACTCA CTGGTGTGTG
    TGTCCAGAAG GATTTGAGGG TGATGAATGT GAGATAAACA TTGACGACTG TGAGGATAAT
    GACTGTGAGA ATAACTCTAC ATGCGTGGAC GGTATCAACA ACTATACCTG TCTCTGCTCA
    CCTGAATACA CAGCCGCTAA TAATAATGAA GTGGAGAAAG GGGAGCTGTG TGAAGATAAG
    CTGGATTTTT GTGCTTCAGA GCTTAACCTG TGTCAGCATG ACTCCAAATG TATTCTGACT
    GCTAAAGGCT TCATGTGTGA ATGCACTCCA GGTTATACCG GTGAGCACTG TGAGGTGGAT
    TTTGATGATT GTGAAGATAA TAAGTGTAAA AACGGGGCTC AGTGTATTGA TGCTGTGAAC
    GGCTACACGT GTGTGTGTCC AGAAGGCTAC AGTGGGTTGT TCTGCGAGTT TTCTCCTCCG
    ATGGTCCTGC CAAGAACAAG TCCATGCGAT CACTACGACT GTGCCAACGG TGCACAGTGT
    GTTGTGAAGG ACACGGACCC CGTGTGTCAG TGTCTGCCAG GTTATGAGGG TGTGCACTGT
    GAAAGACTGG TCAGCGTCAA CTTCATCAAC AGAGAATCCT TCCTGCAAAT CCCCTCCAAC
    CTCATTACAG AACAGGCCAA TATATCTCTG CAGATCGCCA CAGATGAGGA TAATGGAGTG
    TTGCTGTACA AAGGAGATAA TGAACACATT GCTGTGGAGC TTTACAGAGG CCGACTGAGA
    GTCAGTTATG ACTCTGGATC ATATCCTCCA TCTGCAATCT ACAGTGTCGA GACAATAAAT
    GATGGCAGCT TTCATGTAGT GGAGCTGGTG GCAAAGGATC AGTCTCTGTC ACTGTCCATT
    GATGGAGGAA GCCCGAAATC CATCAACACT GCCAATAGCC CCAGCCCAGT GCCCAGCCCG
    GCCCCGCTTT ATCTTGGTGG TCTTCCTCAG CAGTCTGGAT TGGCCTCGTT GCGTCAGGGC
    TCTGGCAGGA ATGGCTCTAG TTTCCATGGC TGCATCCGAA ATCTCTACAT TAATGACCAA
    CTCCAGGATC TCACCCAGTT TTTGCTGCAG GAGGGTGTTG TGCCCGGATG CCAGCCATGC
    CAGCGGAGCA TGTGTGCTCA TGGCCAGTGC CATGCCACTG GACAGTCCTC TTTCAGCTGT
    GAGTGTGAGG CGGGATGGAC TGGCCCGCTC TGTGACCAGC AGGTCAACAA CCCATGTGAC
    GGGAACAAGT GTATCCATGG CTCGTGTATG GCCATAAACT CATACTCGTA CAGCTGCCGT
    TGTCTTCCAG GCTTTGCAGG TGTGCTGTGT GATGAAGAGG AGCAGCTCAG CCCCTGTCAG
    TATATAGCAT GCAAATACGG CCGATGTCGT GTGTCTGGAC TGGGCAAAGC CTATTGTGAG
    TGCAACAGCG GCTACACGGG GCAAAGCTGC GACAGAGAGA TGGCGTGTCG GGGGGAACGG
    GTCCGAGATC ACTATCAGAC GCAGCAGGGC TACGCCGCGT GCCAGAGCAC AGAGAAGGTC
    TCGCGTTTAG AGTGTCGGGG CAGCTGTGGG GACGGGACCT CCTGCTGCGC CCCGCTCCGC
    AGCAAACGCA GGAAATACAC CTTCCAATGC ACAGACGGGT CCTCTTTTGT ACAGGAGGTG
    GAAAAGGTTG TCAAGTGTGG CTGCACAAAA TGTCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331531.1
    CDS827..5428
    Translation

    Target ORF information:

    RefSeq Version XM_021475856.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475856.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGCG TAGAAGACTA CCGCTCAAAA CTAGGTGGGG ATTGCTTTGC TGATTTGGCC
    TGTCCAGAGA AATGTCGATG TGAAGGAACC ACAGTGGACT GCTCTGGACA AAAGCTCACC
    AAGATCCCAG ACCACATCCC TCAGTATACA GCAGAACTCC GCTTGAATAA TAATGAATTC
    ACAGTGCTGG AGGCTACAGG AATCTTCAAG AAGCTTCCTC AGTTGCGCAA AATCAATCTT
    AGCAACAATA AGATCACAGA TATCGAAGAA GGCACATTTG AAGGGGCCAG TGGAGTAAAT
    GAACTCATAC TGACCAGCAA TCGCTTGGAG GGGGTTCACT ACAGCATGCT GAAAGGCCTC
    GGTGGTCTCC GCACACTGAT GTTGAGGAGT AACAGGATAA GCTGTGTCAA TAACGGCAGT
    TTTACCGGTC TGAGCTCTGT GAGGTTGCTT TCCCTCTATG ATAACCTGAT CACCTCCATG
    TCCCCAGGAG CCTTTGACAC ACTACATTCG CTCTCCACAT TGAACTTGTT GGCCAATCCG
    TTTAACTGTA ACTGCCATCT GGCCTGGCTT GGTGAATGGC TCCGCAAGAA ACGGATTGTC
    ACAGGCAACC CCCGCTGTCA GAGCCCCTAC TTTCTCAAGG AGATCCCTAT TCAAGATGTA
    GCTGTACAAG ACTTTGCCTG TGAAGAAGGT AACGATGAGA ATAGCTGTTC ACCACTGGCT
    CGCTGTCCTG CCGAGTGTTC GTGTTTGGAT ACAGTGGTTC GTTGCAGTAA TAAAGGACTC
    AAGGTTTTGC CTAAGGGCAT TCCTAGAGAC GTTACAGAGC TCTATCTCGA TGGCAACGAG
    TTCACACAAG TCCCTCTGGA ACTATCCAAT TATAAACACC TCACTCTCAT TGATTTGAGT
    AATAACCAGA TCAGCACATT GTCCAACCAC AGCTTCAGTA ACATGTCCGA GCTGCTCACA
    CTAATTTTAA GCTACAACAG ATTAAGGTGT ATTCCTGCAA AAGCGTTCGA CGGCCTCAAG
    TCTCTGCGCT TGTTGTCTCT CCATGGTAAT GATATTGCTG TGATCCCAGA TGGAGCTTTC
    AAAGATCTGT CCTCACTCTC TCACCTGGCT CTGGGCGCAA ACCCCCTGTA CTGTGACTGT
    CACATGCAGT GGCTCTCTGA CTGGGTAAAG AGTGGATACA AGGAGCCTGG CATCGCCCGC
    TGTACCGGTC CTGGAGACAT GACTGACAAA CTGCTGCTCA CTACACCCTC TAAGAAATTC
    ACATGCACAG GTCCAGTGGA TGTAAGCATT TTGGCTAAAT GTAACCCTTG CTTGTCCAAT
    CCCTGCAAGA ATGATGGGAC CTGCAGTAAC CATCCTGTAG ATTTTTACAG ATGCACCTGC
    CCGTATGGTT TTAAGGGTCA AGACTGTGAG GAACCAATTC ATGCATGCAT CAGTAACCCC
    TGTCAGAATG GAGGAACCTG TCATCTGAAG GATGGAGAGG AAAACACTCA CTGGTGTGTG
    TGTCCAGAAG GATTTGAGGG TGATGAATGT GAGATAAACA TTGACGACTG TGAGGATAAT
    GACTGTGAGA ATAACTCTAC ATGCGTGGAC GGTATCAACA ACTATACCTG TCTCTGCTCA
    CCTGAATACA CAGCCGCTAA TAATAATGAA GTGGAGAAAG GGGAGCTGTG TGAAGATAAG
    CTGGATTTTT GTGCTTCAGA GCTTAACCTG TGTCAGCATG ACTCCAAATG TATTCTGACT
    GCTAAAGGCT TCATGTGTGA ATGCACTCCA GGTTATACCG GTGAGCACTG TGAGGTGGAT
    TTTGATGATT GTGAAGATAA TAAGTGTAAA AACGGGGCTC AGTGTATTGA TGCTGTGAAC
    GGCTACACGT GTGTGTGTCC AGAAGGCTAC AGTGGGTTGT TCTGCGAGTT TTCTCCTCCG
    ATGGTCCTGC CAAGAACAAG TCCATGCGAT CACTACGACT GTGCCAACGG TGCACAGTGT
    GTTGTGAAGG ACACGGACCC CGTGTGTCAG TGTCTGCCAG GTTATGAGGG TGTGCACTGT
    GAAAGACTGG TCAGCGTCAA CTTCATCAAC AGAGAATCCT TCCTGCAAAT CCCCTCCAAC
    CTCATTACAG AACAGGCCAA TATATCTCTG CAGATCGCCA CAGATGAGGA TAATGGAGTG
    TTGCTGTACA AAGGAGATAA TGAACACATT GCTGTGGAGC TTTACAGAGG CCGACTGAGA
    GTCAGTTATG ACTCTGGATC ATATCCTCCA TCTGCAATCT ACAGTGTCGA GACAATAAAT
    GATGGCAGCT TTCATGTAGT GGAGCTGGTG GCAAAGGATC AGTCTCTGTC ACTGTCCATT
    GATGGAGGAA GCCCGAAATC CATCAACACT GCCAATAGCC CCAGCCCAGT GCCCAGCCCG
    GCCCCGCTTT ATCTTGGTGG TCTTCCTCAG CAGTCTGGAT TGGCCTCGTT GCGTCAGGGC
    TCTGGCAGGA ATGGCTCTAG TTTCCATGGC TGCATCCGAA ATCTCTACAT TAATGACCAA
    CTCCAGGATC TCACCCAGTT TTTGCTGCAG GAGGGTGTTG TGCCCGGATG CCAGCCATGC
    CAGCGGAGCA TGTGTGCTCA TGGCCAGTGC CATGCCACTG GACAGTCCTC TTTCAGCTGT
    GAGTGTGAGG CGGGATGGAC TGGCCCGCTC TGTGACCAGC AGGTCAACAA CCCATGTGAC
    GGGAACAAGT GTATCCATGG CTCGTGTATG GCCATAAACT CATACTCGTA CAGCTGCCGT
    TGTCTTCCAG GCTTTGCAGG TGTGCTGTGT GATGAAGAGG AGCAGCTCAG CCCCTGTCAG
    TATATAGCAT GCAAATACGG CCGATGTCGT GTGTCTGGAC TGGGCAAAGC CTATTGTGAG
    TGCAACAGCG GCTACACGGG GCAAAGCTGC GACAGAGAGA TGGCGTGTCG GGGGGAACGG
    GTCCGAGATC ACTATCAGAC GCAGCAGGGC TACGCCGCGT GCCAGAGCAC AGAGAAGGTC
    TCGCGTTTAG AGTGTCGGGG CAGCTGTGGG GACGGGACCT CCTGCTGCGC CCCGCTCCGC
    AGCAAACGCA GGAAATACAC CTTCCAATGC ACAGACGGGT CCTCTTTTGT ACAGGAGGTG
    GAAAAGGTTG TCAAGTGTGG CTGCACAAAA TGTCCATCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147301
    Clone ID Related Accession (Same CDS sequence) XM_021475857.1
    Accession Version XM_021475857.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGAGGTGC CGGGGACTTG TGGTTTTCCT
    GTGCCAGGTC CAGTGGATGT AAGCATTTTG GCTAAATGTA ACCCTTGCTT GTCCAATCCC
    TGCAAGAATG ATGGGACCTG CAGTAACCAT CCTGTAGATT TTTACAGATG CACCTGCCCG
    TATGGTTTTA AGGGTCAAGA CTGTGAGGAA CCAATTCATG CATGCATCAG TAACCCCTGT
    CAGAATGGAG GAACCTGTCA TCTGAAGGAT GGAGAGGAAA ACACTCACTG GTGTGTGTGT
    CCAGAAGGAT TTGAGGGTGA TGAATGTGAG ATAAACATTG ACGACTGTGA GGATAATGAC
    TGTGAGAATA ACTCTACATG CGTGGACGGT ATCAACAACT ATACCTGTCT CTGCTCACCT
    GAATACACAG CCGCTAATAA TAATGAAGTG GAGAAAGGGG AGCTGTGTGA AGATAAGCTG
    GATTTTTGTG CTTCAGAGCT TAACCTGTGT CAGCATGACT CCAAATGTAT TCTGACTGCT
    AAAGGCTTCA TGTGTGAATG CACTCCAGGT TATACCGGTG AGCACTGTGA GGTGGATTTT
    GATGATTGTG AAGATAATAA GTGTAAAAAC GGGGCTCAGT GTATTGATGC TGTGAACGGC
    TACACGTGTG TGTGTCCAGA AGGCTACAGT GGGTTGTTCT GCGAGTTTTC TCCTCCGATG
    GTCCTGCCAA GAACAAGTCC ATGCGATCAC TACGACTGTG CCAACGGTGC ACAGTGTGTT
    GTGAAGGACA CGGACCCCGT GTGTCAGTGT CTGCCAGGTT ATGAGGGTGT GCACTGTGAA
    AGACTGGTCA GCGTCAACTT CATCAACAGA GAATCCTTCC TGCAAATCCC CTCCAACCTC
    ATTACAGAAC AGGCCAATAT ATCTCTGCAG ATCGCCACAG ATGAGGATAA TGGAGTGTTG
    CTGTACAAAG GAGATAATGA ACACATTGCT GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC
    AGTTATGACT CTGGATCATA TCCTCCATCT GCAATCTACA GTGTCGAGAC AATAAATGAT
    GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA AAGGATCAGT CTCTGTCACT GTCCATTGAT
    GGAGGAAGCC CGAAATCCAT CAACACTGCC AATAGCCCCA GCCCAGTGCC CAGCCCGGCC
    CCGCTTTATC TTGGTGGTCT TCCTCAGCAG TCTGGATTGG CCTCGTTGCG TCAGGGCTCT
    GGCAGGAATG GCTCTAGTTT CCATGGCTGC ATCCGAAATC TCTACATTAA TGACCAACTC
    CAGGATCTCA CCCAGTTTTT GCTGCAGGAG GGTGTTGTGC CCGGATGCCA GCCATGCCAG
    CGGAGCATGT GTGCTCATGG CCAGTGCCAT GCCACTGGAC AGTCCTCTTT CAGCTGTGAG
    TGTGAGGCGG GATGGACTGG CCCGCTCTGT GACCAGCAGG TCAACAACCC ATGTGACGGG
    AACAAGTGTA TCCATGGCTC GTGTATGGCC ATAAACTCAT ACTCGTACAG CTGCCGTTGT
    CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT
    ATAGCATGCA AATACGGCCG ATGTCGTGTG TCTGGACTGG GCAAAGCCTA TTGTGAGTGC
    AACAGCGGCT ACACGGGGCA AAGCTGCGAC AGAGAGATGG CGTGTCGGGG GGAACGGGTC
    CGAGATCACT ATCAGACGCA GCAGGGCTAC GCCGCGTGCC AGAGCACAGA GAAGGTCTCG
    CGTTTAGAGT GTCGGGGCAG CTGTGGGGAC GGGACCTCCT GCTGCGCCCC GCTCCGCAGC
    AAACGCAGGA AATACACCTT CCAATGCACA GACGGGTCCT CTTTTGTACA GGAGGTGGAA
    AAGGTTGTCA AGTGTGGCTG CACAAAATGT CCATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331532.1
    CDS827..5425
    Translation

    Target ORF information:

    RefSeq Version XM_021475857.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475857.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGAGGTGC CGGGGACTTG TGGTTTTCCT
    GTGCCAGGTC CAGTGGATGT AAGCATTTTG GCTAAATGTA ACCCTTGCTT GTCCAATCCC
    TGCAAGAATG ATGGGACCTG CAGTAACCAT CCTGTAGATT TTTACAGATG CACCTGCCCG
    TATGGTTTTA AGGGTCAAGA CTGTGAGGAA CCAATTCATG CATGCATCAG TAACCCCTGT
    CAGAATGGAG GAACCTGTCA TCTGAAGGAT GGAGAGGAAA ACACTCACTG GTGTGTGTGT
    CCAGAAGGAT TTGAGGGTGA TGAATGTGAG ATAAACATTG ACGACTGTGA GGATAATGAC
    TGTGAGAATA ACTCTACATG CGTGGACGGT ATCAACAACT ATACCTGTCT CTGCTCACCT
    GAATACACAG CCGCTAATAA TAATGAAGTG GAGAAAGGGG AGCTGTGTGA AGATAAGCTG
    GATTTTTGTG CTTCAGAGCT TAACCTGTGT CAGCATGACT CCAAATGTAT TCTGACTGCT
    AAAGGCTTCA TGTGTGAATG CACTCCAGGT TATACCGGTG AGCACTGTGA GGTGGATTTT
    GATGATTGTG AAGATAATAA GTGTAAAAAC GGGGCTCAGT GTATTGATGC TGTGAACGGC
    TACACGTGTG TGTGTCCAGA AGGCTACAGT GGGTTGTTCT GCGAGTTTTC TCCTCCGATG
    GTCCTGCCAA GAACAAGTCC ATGCGATCAC TACGACTGTG CCAACGGTGC ACAGTGTGTT
    GTGAAGGACA CGGACCCCGT GTGTCAGTGT CTGCCAGGTT ATGAGGGTGT GCACTGTGAA
    AGACTGGTCA GCGTCAACTT CATCAACAGA GAATCCTTCC TGCAAATCCC CTCCAACCTC
    ATTACAGAAC AGGCCAATAT ATCTCTGCAG ATCGCCACAG ATGAGGATAA TGGAGTGTTG
    CTGTACAAAG GAGATAATGA ACACATTGCT GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC
    AGTTATGACT CTGGATCATA TCCTCCATCT GCAATCTACA GTGTCGAGAC AATAAATGAT
    GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA AAGGATCAGT CTCTGTCACT GTCCATTGAT
    GGAGGAAGCC CGAAATCCAT CAACACTGCC AATAGCCCCA GCCCAGTGCC CAGCCCGGCC
    CCGCTTTATC TTGGTGGTCT TCCTCAGCAG TCTGGATTGG CCTCGTTGCG TCAGGGCTCT
    GGCAGGAATG GCTCTAGTTT CCATGGCTGC ATCCGAAATC TCTACATTAA TGACCAACTC
    CAGGATCTCA CCCAGTTTTT GCTGCAGGAG GGTGTTGTGC CCGGATGCCA GCCATGCCAG
    CGGAGCATGT GTGCTCATGG CCAGTGCCAT GCCACTGGAC AGTCCTCTTT CAGCTGTGAG
    TGTGAGGCGG GATGGACTGG CCCGCTCTGT GACCAGCAGG TCAACAACCC ATGTGACGGG
    AACAAGTGTA TCCATGGCTC GTGTATGGCC ATAAACTCAT ACTCGTACAG CTGCCGTTGT
    CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT
    ATAGCATGCA AATACGGCCG ATGTCGTGTG TCTGGACTGG GCAAAGCCTA TTGTGAGTGC
    AACAGCGGCT ACACGGGGCA AAGCTGCGAC AGAGAGATGG CGTGTCGGGG GGAACGGGTC
    CGAGATCACT ATCAGACGCA GCAGGGCTAC GCCGCGTGCC AGAGCACAGA GAAGGTCTCG
    CGTTTAGAGT GTCGGGGCAG CTGTGGGGAC GGGACCTCCT GCTGCGCCCC GCTCCGCAGC
    AAACGCAGGA AATACACCTT CCAATGCACA GACGGGTCCT CTTTTGTACA GGAGGTGGAA
    AAGGTTGTCA AGTGTGGCTG CACAAAATGT CCATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147302
    Clone ID Related Accession (Same CDS sequence) XM_021475858.1
    Accession Version XM_021475858.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4584bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TAATTTTAAG CTACAACAGA TTAAGGTGTA TTCCTGCAAA AGCGTTCGAC
    GGCCTCAAGT CTCTGCGCTT GTTGTCTCTC CATGGTAATG ATATTGCTGT GATCCCAGAT
    GGAGCTTTCA AAGATCTGTC CTCACTCTCT CACCTGGCTC TGGGCGCAAA CCCCCTGTAC
    TGTGACTGTC ACATGCAGTG GCTCTCTGAC TGGGTAAAGA GTGGATACAA GGAGCCTGGC
    ATCGCCCGCT GTACCGGTCC TGGAGACATG ACTGACAAAC TGCTGCTCAC TACACCCTCT
    AAGAAATTCA CATGCACAGA GGTGCCGGGG ACTTGTGGTT TTCCTGTGCC AGGTCCAGTG
    GATGTAAGCA TTTTGGCTAA ATGTAACCCT TGCTTGTCCA ATCCCTGCAA GAATGATGGG
    ACCTGCAGTA ACCATCCTGT AGATTTTTAC AGATGCACCT GCCCGTATGG TTTTAAGGGT
    CAAGACTGTG AGGAACCAAT TCATGCATGC ATCAGTAACC CCTGTCAGAA TGGAGGAACC
    TGTCATCTGA AGGATGGAGA GGAAAACACT CACTGGTGTG TGTGTCCAGA AGGATTTGAG
    GGTGATGAAT GTGAGATAAA CATTGACGAC TGTGAGGATA ATGACTGTGA GAATAACTCT
    ACATGCGTGG ACGGTATCAA CAACTATACC TGTCTCTGCT CACCTGAATA CACAGCCGCT
    AATAATAATG AAGTGGAGAA AGGGGAGCTG TGTGAAGATA AGCTGGATTT TTGTGCTTCA
    GAGCTTAACC TGTGTCAGCA TGACTCCAAA TGTATTCTGA CTGCTAAAGG CTTCATGTGT
    GAATGCACTC CAGGTTATAC CGGTGAGCAC TGTGAGGTGG ATTTTGATGA TTGTGAAGAT
    AATAAGTGTA AAAACGGGGC TCAGTGTATT GATGCTGTGA ACGGCTACAC GTGTGTGTGT
    CCAGAAGGCT ACAGTGGGTT GTTCTGCGAG TTTTCTCCTC CGATGGTCCT GCCAAGAACA
    AGTCCATGCG ATCACTACGA CTGTGCCAAC GGTGCACAGT GTGTTGTGAA GGACACGGAC
    CCCGTGTGTC AGTGTCTGCC AGGTTATGAG GGTGTGCACT GTGAAAGACT GGTCAGCGTC
    AACTTCATCA ACAGAGAATC CTTCCTGCAA ATCCCCTCCA ACCTCATTAC AGAACAGGCC
    AATATATCTC TGCAGATCGC CACAGATGAG GATAATGGAG TGTTGCTGTA CAAAGGAGAT
    AATGAACACA TTGCTGTGGA GCTTTACAGA GGCCGACTGA GAGTCAGTTA TGACTCTGGA
    TCATATCCTC CATCTGCAAT CTACAGTGTC GAGACAATAA ATGATGGCAG CTTTCATGTA
    GTGGAGCTGG TGGCAAAGGA TCAGTCTCTG TCACTGTCCA TTGATGGAGG AAGCCCGAAA
    TCCATCAACA CTGCCAATAG CCCCAGCCCA GTGCCCAGCC CGGCCCCGCT TTATCTTGGT
    GGTCTTCCTC AGCAGTCTGG ATTGGCCTCG TTGCGTCAGG GCTCTGGCAG GAATGGCTCT
    AGTTTCCATG GCTGCATCCG AAATCTCTAC ATTAATGACC AACTCCAGGA TCTCACCCAG
    TTTTTGCTGC AGGAGGGTGT TGTGCCCGGA TGCCAGCCAT GCCAGCGGAG CATGTGTGCT
    CATGGCCAGT GCCATGCCAC TGGACAGTCC TCTTTCAGCT GTGAGTGTGA GGCGGGATGG
    ACTGGCCCGC TCTGTGACCA GCAGGTCAAC AACCCATGTG ACGGGAACAA GTGTATCCAT
    GGCTCGTGTA TGGCCATAAA CTCATACTCG TACAGCTGCC GTTGTCTTCC AGGCTTTGCA
    GGTGTGCTGT GTGATGAAGA GGAGCAGCTC AGCCCCTGTC AGTATATAGC ATGCAAATAC
    GGCCGATGTC GTGTGTCTGG ACTGGGCAAA GCCTATTGTG AGTGCAACAG CGGCTACACG
    GGGCAAAGCT GCGACAGAGA GATGGCGTGT CGGGGGGAAC GGGTCCGAGA TCACTATCAG
    ACGCAGCAGG GCTACGCCGC GTGCCAGAGC ACAGAGAAGG TCTCGCGTTT AGAGTGTCGG
    GGCAGCTGTG GGGACGGGAC CTCCTGCTGC GCCCCGCTCC GCAGCAAACG CAGGAAATAC
    ACCTTCCAAT GCACAGACGG GTCCTCTTTT GTACAGGAGG TGGAAAAGGT TGTCAAGTGT
    GGCTGCACAA AATGTCCATC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331533.1
    CDS828..5411
    Translation

    Target ORF information:

    RefSeq Version XM_021475858.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475858.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TAATTTTAAG CTACAACAGA TTAAGGTGTA TTCCTGCAAA AGCGTTCGAC
    GGCCTCAAGT CTCTGCGCTT GTTGTCTCTC CATGGTAATG ATATTGCTGT GATCCCAGAT
    GGAGCTTTCA AAGATCTGTC CTCACTCTCT CACCTGGCTC TGGGCGCAAA CCCCCTGTAC
    TGTGACTGTC ACATGCAGTG GCTCTCTGAC TGGGTAAAGA GTGGATACAA GGAGCCTGGC
    ATCGCCCGCT GTACCGGTCC TGGAGACATG ACTGACAAAC TGCTGCTCAC TACACCCTCT
    AAGAAATTCA CATGCACAGA GGTGCCGGGG ACTTGTGGTT TTCCTGTGCC AGGTCCAGTG
    GATGTAAGCA TTTTGGCTAA ATGTAACCCT TGCTTGTCCA ATCCCTGCAA GAATGATGGG
    ACCTGCAGTA ACCATCCTGT AGATTTTTAC AGATGCACCT GCCCGTATGG TTTTAAGGGT
    CAAGACTGTG AGGAACCAAT TCATGCATGC ATCAGTAACC CCTGTCAGAA TGGAGGAACC
    TGTCATCTGA AGGATGGAGA GGAAAACACT CACTGGTGTG TGTGTCCAGA AGGATTTGAG
    GGTGATGAAT GTGAGATAAA CATTGACGAC TGTGAGGATA ATGACTGTGA GAATAACTCT
    ACATGCGTGG ACGGTATCAA CAACTATACC TGTCTCTGCT CACCTGAATA CACAGCCGCT
    AATAATAATG AAGTGGAGAA AGGGGAGCTG TGTGAAGATA AGCTGGATTT TTGTGCTTCA
    GAGCTTAACC TGTGTCAGCA TGACTCCAAA TGTATTCTGA CTGCTAAAGG CTTCATGTGT
    GAATGCACTC CAGGTTATAC CGGTGAGCAC TGTGAGGTGG ATTTTGATGA TTGTGAAGAT
    AATAAGTGTA AAAACGGGGC TCAGTGTATT GATGCTGTGA ACGGCTACAC GTGTGTGTGT
    CCAGAAGGCT ACAGTGGGTT GTTCTGCGAG TTTTCTCCTC CGATGGTCCT GCCAAGAACA
    AGTCCATGCG ATCACTACGA CTGTGCCAAC GGTGCACAGT GTGTTGTGAA GGACACGGAC
    CCCGTGTGTC AGTGTCTGCC AGGTTATGAG GGTGTGCACT GTGAAAGACT GGTCAGCGTC
    AACTTCATCA ACAGAGAATC CTTCCTGCAA ATCCCCTCCA ACCTCATTAC AGAACAGGCC
    AATATATCTC TGCAGATCGC CACAGATGAG GATAATGGAG TGTTGCTGTA CAAAGGAGAT
    AATGAACACA TTGCTGTGGA GCTTTACAGA GGCCGACTGA GAGTCAGTTA TGACTCTGGA
    TCATATCCTC CATCTGCAAT CTACAGTGTC GAGACAATAA ATGATGGCAG CTTTCATGTA
    GTGGAGCTGG TGGCAAAGGA TCAGTCTCTG TCACTGTCCA TTGATGGAGG AAGCCCGAAA
    TCCATCAACA CTGCCAATAG CCCCAGCCCA GTGCCCAGCC CGGCCCCGCT TTATCTTGGT
    GGTCTTCCTC AGCAGTCTGG ATTGGCCTCG TTGCGTCAGG GCTCTGGCAG GAATGGCTCT
    AGTTTCCATG GCTGCATCCG AAATCTCTAC ATTAATGACC AACTCCAGGA TCTCACCCAG
    TTTTTGCTGC AGGAGGGTGT TGTGCCCGGA TGCCAGCCAT GCCAGCGGAG CATGTGTGCT
    CATGGCCAGT GCCATGCCAC TGGACAGTCC TCTTTCAGCT GTGAGTGTGA GGCGGGATGG
    ACTGGCCCGC TCTGTGACCA GCAGGTCAAC AACCCATGTG ACGGGAACAA GTGTATCCAT
    GGCTCGTGTA TGGCCATAAA CTCATACTCG TACAGCTGCC GTTGTCTTCC AGGCTTTGCA
    GGTGTGCTGT GTGATGAAGA GGAGCAGCTC AGCCCCTGTC AGTATATAGC ATGCAAATAC
    GGCCGATGTC GTGTGTCTGG ACTGGGCAAA GCCTATTGTG AGTGCAACAG CGGCTACACG
    GGGCAAAGCT GCGACAGAGA GATGGCGTGT CGGGGGGAAC GGGTCCGAGA TCACTATCAG
    ACGCAGCAGG GCTACGCCGC GTGCCAGAGC ACAGAGAAGG TCTCGCGTTT AGAGTGTCGG
    GGCAGCTGTG GGGACGGGAC CTCCTGCTGC GCCCCGCTCC GCAGCAAACG CAGGAAATAC
    ACCTTCCAAT GCACAGACGG GTCCTCTTTT GTACAGGAGG TGGAAAAGGT TGTCAAGTGT
    GGCTGCACAA AATGTCCATC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147303
    Clone ID Related Accession (Same CDS sequence) XM_021475859.1
    Accession Version XM_021475859.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGCG TAGAAGACTA CCGCTCAAAA CTAGGTGGGG ATTGCTTTGC TGATTTGGCC
    TGTCCAGAGA AATGTCGATG TGAAGGAACC ACAGTGGACT GCTCTGGACA AAAGCTCACC
    AAGATCCCAG ACCACATCCC TCAGTATACA GCAGAACTCC GCTTGAATAA TAATGAATTC
    ACAGTGCTGG AGGCTACAGG AATCTTCAAG AAGCTTCCTC AGTTGCGCAA AATCAATCTT
    AGCAACAATA AGATCACAGA TATCGAAGAA GGCACATTTG AAGGGGCCAG TGGAGTAAAT
    GAACTCATAC TGACCAGCAA TCGCTTGGAG GGGGTTCACT ACAGCATGCT GAAAGGCCTC
    GGTGGTCTCC GCACACTGAT GTTGAGGAGT AACAGGATAA GCTGTGTCAA TAACGGCAGT
    TTTACCGGTC TGAGCTCTGT GAGGTTGCTT TCCCTCTATG ATAACCTGAT CACCTCCATG
    TCCCCAGGAG CCTTTGACAC ACTACATTCG CTCTCCACAT TGAACTTGTT GGCCAATCCG
    TTTAACTGTA ACTGCCATCT GGCCTGGCTT GGTGAATGGC TCCGCAAGAA ACGGATTGTC
    ACAGGCAACC CCCGCTGTCA GAGCCCCTAC TTTCTCAAGG AGATCCCTAT TCAAGATGTA
    GCTGTACAAG ACTTTGCCTG TGAAGAAGGT AACGATGAGA ATAGCTGTTC ACCACTGGCT
    CGCTGTCCTG CCGAGTGTTC GTGTTTGGAT ACAGTGGTTC GTTGCAGTAA TAAAGGACTC
    AAGGTTTTGC CTAAGGGCAT TCCTAGAGAC GTTACAGAGC TCTATCTCGA TGGCAACGAG
    TTCACACAAG TCCCTCTGGA ACTATCCAAT TATAAACACC TCACTCTCAT TGATTTGAGT
    AATAACCAGA TCAGCACATT GTCCAACCAC AGCTTCAGTA ACATGTCCGA GCTGCTCACA
    CTAATTTTAA GCTACAACAG ATTAAGGTGT ATTCCTGCAA AAGCGTTCGA CGGCCTCAAG
    TCTCTGCGCT TGTTGTCTCT CCATGGTAAT GATATTGCTG TGATCCCAGA TGGAGCTTTC
    AAAGATCTGT CCTCACTCTC TCACCTGGCT CTGGGCGCAA ACCCCCTGTA CTGTGACTGT
    CACATGCAGT GGCTCTCTGA CTGGGTAAAG AGTGGATACA AGGAGCCTGG CATCGCCCGC
    TGTACCGGTC CTGGAGACAT GACTGACAAA CTGCTGCTCA CTACACCCTC TAAGAAATTC
    ACATGCACAG GTCCAGTGGA TGTAAGCATT TTGGCTAAAT GTAACCCTTG CTTGTCCAAT
    CCCTGCAAGA ATGATGGGAC CTGCAGTAAC CATCCTGTAG ATTTTTACAG ATGCACCTGC
    CCGTATGGTT TTAAGGGTCA AGACTGTGAG GAACCAATTC ATGCATGCAT CAGTAACCCC
    TGTCAGAATG GAGGAACCTG TCATCTGAAG GATGGAGAGG AAAACACTCA CTGGTGTGTG
    TGTCCAGAAG GATTTGAGGG TGATGAATGT GAGATAAACA TTGACGACTG TGAGGATAAT
    GACTGTGAGA ATAACTCTAC ATGCGTGGAC GGTATCAACA ACTATACCTG TCTCTGCTCA
    CCTGAATACA CAGGGGAGCT GTGTGAAGAT AAGCTGGATT TTTGTGCTTC AGAGCTTAAC
    CTGTGTCAGC ATGACTCCAA ATGTATTCTG ACTGCTAAAG GCTTCATGTG TGAATGCACT
    CCAGGTTATA CCGGTGAGCA CTGTGAGGTG GATTTTGATG ATTGTGAAGA TAATAAGTGT
    AAAAACGGGG CTCAGTGTAT TGATGCTGTG AACGGCTACA CGTGTGTGTG TCCAGAAGGC
    TACAGTGGGT TGTTCTGCGA GTTTTCTCCT CCGATGGTCC TGCCAAGAAC AAGTCCATGC
    GATCACTACG ACTGTGCCAA CGGTGCACAG TGTGTTGTGA AGGACACGGA CCCCGTGTGT
    CAGTGTCTGC CAGGTTATGA GGGTGTGCAC TGTGAAAGAC TGGTCAGCGT CAACTTCATC
    AACAGAGAAT CCTTCCTGCA AATCCCCTCC AACCTCATTA CAGAACAGGC CAATATATCT
    CTGCAGATCG CCACAGATGA GGATAATGGA GTGTTGCTGT ACAAAGGAGA TAATGAACAC
    ATTGCTGTGG AGCTTTACAG AGGCCGACTG AGAGTCAGTT ATGACTCTGG ATCATATCCT
    CCATCTGCAA TCTACAGTGT CGAGACAATA AATGATGGCA GCTTTCATGT AGTGGAGCTG
    GTGGCAAAGG ATCAGTCTCT GTCACTGTCC ATTGATGGAG GAAGCCCGAA ATCCATCAAC
    ACTGCCAATA GCCCCAGCCC AGTGCCCAGC CCGGCCCCGC TTTATCTTGG TGGTCTTCCT
    CAGCAGTCTG GATTGGCCTC GTTGCGTCAG GGCTCTGGCA GGAATGGCTC TAGTTTCCAT
    GGCTGCATCC GAAATCTCTA CATTAATGAC CAACTCCAGG ATCTCACCCA GTTTTTGCTG
    CAGGAGGGTG TTGTGCCCGG ATGCCAGCCA TGCCAGCGGA GCATGTGTGC TCATGGCCAG
    TGCCATGCCA CTGGACAGTC CTCTTTCAGC TGTGAGTGTG AGGCGGGATG GACTGGCCCG
    CTCTGTGACC AGCAGGTCAA CAACCCATGT GACGGGAACA AGTGTATCCA TGGCTCGTGT
    ATGGCCATAA ACTCATACTC GTACAGCTGC CGTTGTCTTC CAGGCTTTGC AGGTGTGCTG
    TGTGATGAAG AGGAGCAGCT CAGCCCCTGT CAGTATATAG CATGCAAATA CGGCCGATGT
    CGTGTGTCTG GACTGGGCAA AGCCTATTGT GAGTGCAACA GCGGCTACAC GGGGCAAAGC
    TGCGACAGAG AGATGGCGTG TCGGGGGGAA CGGGTCCGAG ATCACTATCA GACGCAGCAG
    GGCTACGCCG CGTGCCAGAG CACAGAGAAG GTCTCGCGTT TAGAGTGTCG GGGCAGCTGT
    GGGGACGGGA CCTCCTGCTG CGCCCCGCTC CGCAGCAAAC GCAGGAAATA CACCTTCCAA
    TGCACAGACG GGTCCTCTTT TGTACAGGAG GTGGAAAAGG TTGTCAAGTG TGGCTGCACA
    AAATGTCCAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331534.1
    CDS827..5401
    Translation

    Target ORF information:

    RefSeq Version XM_021475859.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475859.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGCG TAGAAGACTA CCGCTCAAAA CTAGGTGGGG ATTGCTTTGC TGATTTGGCC
    TGTCCAGAGA AATGTCGATG TGAAGGAACC ACAGTGGACT GCTCTGGACA AAAGCTCACC
    AAGATCCCAG ACCACATCCC TCAGTATACA GCAGAACTCC GCTTGAATAA TAATGAATTC
    ACAGTGCTGG AGGCTACAGG AATCTTCAAG AAGCTTCCTC AGTTGCGCAA AATCAATCTT
    AGCAACAATA AGATCACAGA TATCGAAGAA GGCACATTTG AAGGGGCCAG TGGAGTAAAT
    GAACTCATAC TGACCAGCAA TCGCTTGGAG GGGGTTCACT ACAGCATGCT GAAAGGCCTC
    GGTGGTCTCC GCACACTGAT GTTGAGGAGT AACAGGATAA GCTGTGTCAA TAACGGCAGT
    TTTACCGGTC TGAGCTCTGT GAGGTTGCTT TCCCTCTATG ATAACCTGAT CACCTCCATG
    TCCCCAGGAG CCTTTGACAC ACTACATTCG CTCTCCACAT TGAACTTGTT GGCCAATCCG
    TTTAACTGTA ACTGCCATCT GGCCTGGCTT GGTGAATGGC TCCGCAAGAA ACGGATTGTC
    ACAGGCAACC CCCGCTGTCA GAGCCCCTAC TTTCTCAAGG AGATCCCTAT TCAAGATGTA
    GCTGTACAAG ACTTTGCCTG TGAAGAAGGT AACGATGAGA ATAGCTGTTC ACCACTGGCT
    CGCTGTCCTG CCGAGTGTTC GTGTTTGGAT ACAGTGGTTC GTTGCAGTAA TAAAGGACTC
    AAGGTTTTGC CTAAGGGCAT TCCTAGAGAC GTTACAGAGC TCTATCTCGA TGGCAACGAG
    TTCACACAAG TCCCTCTGGA ACTATCCAAT TATAAACACC TCACTCTCAT TGATTTGAGT
    AATAACCAGA TCAGCACATT GTCCAACCAC AGCTTCAGTA ACATGTCCGA GCTGCTCACA
    CTAATTTTAA GCTACAACAG ATTAAGGTGT ATTCCTGCAA AAGCGTTCGA CGGCCTCAAG
    TCTCTGCGCT TGTTGTCTCT CCATGGTAAT GATATTGCTG TGATCCCAGA TGGAGCTTTC
    AAAGATCTGT CCTCACTCTC TCACCTGGCT CTGGGCGCAA ACCCCCTGTA CTGTGACTGT
    CACATGCAGT GGCTCTCTGA CTGGGTAAAG AGTGGATACA AGGAGCCTGG CATCGCCCGC
    TGTACCGGTC CTGGAGACAT GACTGACAAA CTGCTGCTCA CTACACCCTC TAAGAAATTC
    ACATGCACAG GTCCAGTGGA TGTAAGCATT TTGGCTAAAT GTAACCCTTG CTTGTCCAAT
    CCCTGCAAGA ATGATGGGAC CTGCAGTAAC CATCCTGTAG ATTTTTACAG ATGCACCTGC
    CCGTATGGTT TTAAGGGTCA AGACTGTGAG GAACCAATTC ATGCATGCAT CAGTAACCCC
    TGTCAGAATG GAGGAACCTG TCATCTGAAG GATGGAGAGG AAAACACTCA CTGGTGTGTG
    TGTCCAGAAG GATTTGAGGG TGATGAATGT GAGATAAACA TTGACGACTG TGAGGATAAT
    GACTGTGAGA ATAACTCTAC ATGCGTGGAC GGTATCAACA ACTATACCTG TCTCTGCTCA
    CCTGAATACA CAGGGGAGCT GTGTGAAGAT AAGCTGGATT TTTGTGCTTC AGAGCTTAAC
    CTGTGTCAGC ATGACTCCAA ATGTATTCTG ACTGCTAAAG GCTTCATGTG TGAATGCACT
    CCAGGTTATA CCGGTGAGCA CTGTGAGGTG GATTTTGATG ATTGTGAAGA TAATAAGTGT
    AAAAACGGGG CTCAGTGTAT TGATGCTGTG AACGGCTACA CGTGTGTGTG TCCAGAAGGC
    TACAGTGGGT TGTTCTGCGA GTTTTCTCCT CCGATGGTCC TGCCAAGAAC AAGTCCATGC
    GATCACTACG ACTGTGCCAA CGGTGCACAG TGTGTTGTGA AGGACACGGA CCCCGTGTGT
    CAGTGTCTGC CAGGTTATGA GGGTGTGCAC TGTGAAAGAC TGGTCAGCGT CAACTTCATC
    AACAGAGAAT CCTTCCTGCA AATCCCCTCC AACCTCATTA CAGAACAGGC CAATATATCT
    CTGCAGATCG CCACAGATGA GGATAATGGA GTGTTGCTGT ACAAAGGAGA TAATGAACAC
    ATTGCTGTGG AGCTTTACAG AGGCCGACTG AGAGTCAGTT ATGACTCTGG ATCATATCCT
    CCATCTGCAA TCTACAGTGT CGAGACAATA AATGATGGCA GCTTTCATGT AGTGGAGCTG
    GTGGCAAAGG ATCAGTCTCT GTCACTGTCC ATTGATGGAG GAAGCCCGAA ATCCATCAAC
    ACTGCCAATA GCCCCAGCCC AGTGCCCAGC CCGGCCCCGC TTTATCTTGG TGGTCTTCCT
    CAGCAGTCTG GATTGGCCTC GTTGCGTCAG GGCTCTGGCA GGAATGGCTC TAGTTTCCAT
    GGCTGCATCC GAAATCTCTA CATTAATGAC CAACTCCAGG ATCTCACCCA GTTTTTGCTG
    CAGGAGGGTG TTGTGCCCGG ATGCCAGCCA TGCCAGCGGA GCATGTGTGC TCATGGCCAG
    TGCCATGCCA CTGGACAGTC CTCTTTCAGC TGTGAGTGTG AGGCGGGATG GACTGGCCCG
    CTCTGTGACC AGCAGGTCAA CAACCCATGT GACGGGAACA AGTGTATCCA TGGCTCGTGT
    ATGGCCATAA ACTCATACTC GTACAGCTGC CGTTGTCTTC CAGGCTTTGC AGGTGTGCTG
    TGTGATGAAG AGGAGCAGCT CAGCCCCTGT CAGTATATAG CATGCAAATA CGGCCGATGT
    CGTGTGTCTG GACTGGGCAA AGCCTATTGT GAGTGCAACA GCGGCTACAC GGGGCAAAGC
    TGCGACAGAG AGATGGCGTG TCGGGGGGAA CGGGTCCGAG ATCACTATCA GACGCAGCAG
    GGCTACGCCG CGTGCCAGAG CACAGAGAAG GTCTCGCGTT TAGAGTGTCG GGGCAGCTGT
    GGGGACGGGA CCTCCTGCTG CGCCCCGCTC CGCAGCAAAC GCAGGAAATA CACCTTCCAA
    TGCACAGACG GGTCCTCTTT TGTACAGGAG GTGGAAAAGG TTGTCAAGTG TGGCTGCACA
    AAATGTCCAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147304
    Clone ID Related Accession (Same CDS sequence) XM_021475860.1
    Accession Version XM_021475860.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GCTAAAGAGC AATATTTTAT ACCAGGCGTA
    GAAGACTACC GCTCAAAACT AGGTGGGGAT TGCTTTGCTG ATTTGGCCTG TCCAGAGAAA
    TGTCGATGTG AAGGAACCAC AGTGGACTGC TCTGGACAAA AGCTCACCAA GATCCCAGAC
    CACATCCCTC AGTATACAGC AGAACTCCGC TTGAATAATA ATGAATTCAC AGTGCTGGAG
    GCTACAGGAA TCTTCAAGAA GCTTCCTCAG TTGCGCAAAA TCAATCTTAG CAACAATAAG
    ATCACAGATA TCGAAGAAGG CACATTTGAA GGGGCCAGTG GAGTAAATGA ACTCATACTG
    ACCAGCAATC GCTTGGAGGG GGTTCACTAC AGCATGCTGA AAGGCCTCGG TGGTCTCCGC
    ACACTGATGT TGAGGAGTAA CAGGATAAGC TGTGTCAATA ACGGCAGTTT TACCGGTCTG
    AGCTCTGTGA GGTTGCTTTC CCTCTATGAT AACCTGATCA CCTCCATGTC CCCAGGAGCC
    TTTGACACAC TACATTCGCT CTCCACATTG AACTTGTTGG CCAATCCGTT TAACTGTAAC
    TGCCATCTGG CCTGGCTTGG TGAATGGCTC CGCAAGAAAC GGATTGTCAC AGGCAACCCC
    CGCTGTCAGA GCCCCTACTT TCTCAAGGAG ATCCCTATTC AAGATGTAGC TGTACAAGAC
    TTTGCCTGTG AAGAAGGTAA CGATGAGAAT AGCTGTTCAC CACTGGCTCG CTGTCCTGCC
    GAGTGTTCGT GTTTGGATAC AGTGGTTCGT TGCAGTAATA AAGGACTCAA GGTTTTGCCT
    AAGGGCATTC CTAGAGACGT TACAGAGCTC TATCTCGATG GCAACGAGTT CACACAAGTC
    CCTCTGGAAC TATCCAATTA TAAACACCTC ACTCTCATTG ATTTGAGTAA TAACCAGATC
    AGCACATTGT CCAACCACAG CTTCAGTAAC ATGTCCGAGC TGCTCACACT AATTTTAAGC
    TACAACAGAT TAAGGTGTAT TCCTGCAAAA GCGTTCGACG GCCTCAAGTC TCTGCGCTTG
    TTGTCTCTCC ATGGTAATGA TATTGCTGTG ATCCCAGATG GAGCTTTCAA AGATCTGTCC
    TCACTCTCTC ACCTGGCTCT GGGCGCAAAC CCCCTGTACT GTGACTGTCA CATGCAGTGG
    CTCTCTGACT GGGTAAAGAG TGGATACAAG GAGCCTGGCA TCGCCCGCTG TACCGGTCCT
    GGAGACATGA CTGACAAACT GCTGCTCACT ACACCCTCTA AGAAATTCAC ATGCACAGGT
    CCAGTGGATG TAAGCATTTT GGCTAAATGT AACCCTTGCT TGTCCAATCC CTGCAAGAAT
    GATGGGACCT GCAGTAACCA TCCTGTAGAT TTTTACAGAT GCACCTGCCC GTATGGTTTT
    AAGGGTCAAG ACTGTGAGGA ACCAATTCAT GCATGCATCA GTAACCCCTG TCAGAATGGA
    GGAACCTGTC ATCTGAAGGA TGGAGAGGAA AACACTCACT GGTGTGTGTG TCCAGAAGGA
    TTTGAGGGTG ATGAATGTGA GATAAACATT GACGACTGTG AGGATAATGA CTGTGAGAAT
    AACTCTACAT GCGTGGACGG TATCAACAAC TATACCTGTC TCTGCTCACC TGAATACACA
    GGGGAGCTGT GTGAAGATAA GCTGGATTTT TGTGCTTCAG AGCTTAACCT GTGTCAGCAT
    GACTCCAAAT GTATTCTGAC TGCTAAAGGC TTCATGTGTG AATGCACTCC AGGTTATACC
    GGTGAGCACT GTGAGGTGGA TTTTGATGAT TGTGAAGATA ATAAGTGTAA AAACGGGGCT
    CAGTGTATTG ATGCTGTGAA CGGCTACACG TGTGTGTGTC CAGAAGGCTA CAGTGGGTTG
    TTCTGCGAGT TTTCTCCTCC GATGGTCCTG CCAAGAACAA GTCCATGCGA TCACTACGAC
    TGTGCCAACG GTGCACAGTG TGTTGTGAAG GACACGGACC CCGTGTGTCA GTGTCTGCCA
    GGTTATGAGG GTGTGCACTG TGAAAGACTG GTCAGCGTCA ACTTCATCAA CAGAGAATCC
    TTCCTGCAAA TCCCCTCCAA CCTCATTACA GAACAGGCCA ATATATCTCT GCAGATCGCC
    ACAGATGAGG ATAATGGAGT GTTGCTGTAC AAAGGAGATA ATGAACACAT TGCTGTGGAG
    CTTTACAGAG GCCGACTGAG AGTCAGTTAT GACTCTGGAT CATATCCTCC ATCTGCAATC
    TACAGTGTCG AGACAATAAA TGATGGCAGC TTTCATGTAG TGGAGCTGGT GGCAAAGGAT
    CAGTCTCTGT CACTGTCCAT TGATGGAGGA AGCCCGAAAT CCATCAACAC TGCCAATAGC
    CCCAGCCCAG TGCCCAGCCC GGCCCCGCTT TATCTTGGTG GTCTTCCTCA GCAGTCTGGA
    TTGGCCTCGT TGCGTCAGGG CTCTGGCAGG AATGGCTCTA GTTTCCATGG CTGCATCCGA
    AATCTCTACA TTAATGACCA ACTCCAGGAT CTCACCCAGT TTTTGCTGCA GGAGGGTGTT
    GTGCCCGGAT GCCAGCCATG CCAGCGGAGC ATGTGTGCTC ATGGCCAGTG CCATGCCACT
    GGACAGTCCT CTTTCAGCTG TGAGTGTGAG GCGGGATGGA CTGGCCCGCT CTGTGACCAG
    CAGGTCAACA ACCCATGTGA CGGGAACAAG TGTATCCATG GCTCGTGTAT GGCCATAAAC
    TCATACTCGT ACAGCTGCCG TTGTCTTCCA GGCTTTGCAG GTGTGCTGTG TGATGAAGAG
    GAGCAGCTCA GCCCCTGTCA GTATATAGCA TGCAAATACG GCCGATGTCG TGTGTCTGGA
    CTGGGCAAAG CCTATTGTGA GTGCAACAGC GGCTACACGG GGCAAAGCTG CGACAGAGAG
    ATGGCGTGTC GGGGGGAACG GGTCCGAGAT CACTATCAGA CGCAGCAGGG CTACGCCGCG
    TGCCAGAGCA CAGAGAAGGT CTCGCGTTTA GAGTGTCGGG GCAGCTGTGG GGACGGGACC
    TCCTGCTGCG CCCCGCTCCG CAGCAAACGC AGGAAATACA CCTTCCAATG CACAGACGGG
    TCCTCTTTTG TACAGGAGGT GGAAAAGGTT GTCAAGTGTG GCTGCACAAA ATGTCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331535.1
    CDS827..5389
    Translation

    Target ORF information:

    RefSeq Version XM_021475860.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GCTAAAGAGC AATATTTTAT ACCAGGCGTA
    GAAGACTACC GCTCAAAACT AGGTGGGGAT TGCTTTGCTG ATTTGGCCTG TCCAGAGAAA
    TGTCGATGTG AAGGAACCAC AGTGGACTGC TCTGGACAAA AGCTCACCAA GATCCCAGAC
    CACATCCCTC AGTATACAGC AGAACTCCGC TTGAATAATA ATGAATTCAC AGTGCTGGAG
    GCTACAGGAA TCTTCAAGAA GCTTCCTCAG TTGCGCAAAA TCAATCTTAG CAACAATAAG
    ATCACAGATA TCGAAGAAGG CACATTTGAA GGGGCCAGTG GAGTAAATGA ACTCATACTG
    ACCAGCAATC GCTTGGAGGG GGTTCACTAC AGCATGCTGA AAGGCCTCGG TGGTCTCCGC
    ACACTGATGT TGAGGAGTAA CAGGATAAGC TGTGTCAATA ACGGCAGTTT TACCGGTCTG
    AGCTCTGTGA GGTTGCTTTC CCTCTATGAT AACCTGATCA CCTCCATGTC CCCAGGAGCC
    TTTGACACAC TACATTCGCT CTCCACATTG AACTTGTTGG CCAATCCGTT TAACTGTAAC
    TGCCATCTGG CCTGGCTTGG TGAATGGCTC CGCAAGAAAC GGATTGTCAC AGGCAACCCC
    CGCTGTCAGA GCCCCTACTT TCTCAAGGAG ATCCCTATTC AAGATGTAGC TGTACAAGAC
    TTTGCCTGTG AAGAAGGTAA CGATGAGAAT AGCTGTTCAC CACTGGCTCG CTGTCCTGCC
    GAGTGTTCGT GTTTGGATAC AGTGGTTCGT TGCAGTAATA AAGGACTCAA GGTTTTGCCT
    AAGGGCATTC CTAGAGACGT TACAGAGCTC TATCTCGATG GCAACGAGTT CACACAAGTC
    CCTCTGGAAC TATCCAATTA TAAACACCTC ACTCTCATTG ATTTGAGTAA TAACCAGATC
    AGCACATTGT CCAACCACAG CTTCAGTAAC ATGTCCGAGC TGCTCACACT AATTTTAAGC
    TACAACAGAT TAAGGTGTAT TCCTGCAAAA GCGTTCGACG GCCTCAAGTC TCTGCGCTTG
    TTGTCTCTCC ATGGTAATGA TATTGCTGTG ATCCCAGATG GAGCTTTCAA AGATCTGTCC
    TCACTCTCTC ACCTGGCTCT GGGCGCAAAC CCCCTGTACT GTGACTGTCA CATGCAGTGG
    CTCTCTGACT GGGTAAAGAG TGGATACAAG GAGCCTGGCA TCGCCCGCTG TACCGGTCCT
    GGAGACATGA CTGACAAACT GCTGCTCACT ACACCCTCTA AGAAATTCAC ATGCACAGGT
    CCAGTGGATG TAAGCATTTT GGCTAAATGT AACCCTTGCT TGTCCAATCC CTGCAAGAAT
    GATGGGACCT GCAGTAACCA TCCTGTAGAT TTTTACAGAT GCACCTGCCC GTATGGTTTT
    AAGGGTCAAG ACTGTGAGGA ACCAATTCAT GCATGCATCA GTAACCCCTG TCAGAATGGA
    GGAACCTGTC ATCTGAAGGA TGGAGAGGAA AACACTCACT GGTGTGTGTG TCCAGAAGGA
    TTTGAGGGTG ATGAATGTGA GATAAACATT GACGACTGTG AGGATAATGA CTGTGAGAAT
    AACTCTACAT GCGTGGACGG TATCAACAAC TATACCTGTC TCTGCTCACC TGAATACACA
    GGGGAGCTGT GTGAAGATAA GCTGGATTTT TGTGCTTCAG AGCTTAACCT GTGTCAGCAT
    GACTCCAAAT GTATTCTGAC TGCTAAAGGC TTCATGTGTG AATGCACTCC AGGTTATACC
    GGTGAGCACT GTGAGGTGGA TTTTGATGAT TGTGAAGATA ATAAGTGTAA AAACGGGGCT
    CAGTGTATTG ATGCTGTGAA CGGCTACACG TGTGTGTGTC CAGAAGGCTA CAGTGGGTTG
    TTCTGCGAGT TTTCTCCTCC GATGGTCCTG CCAAGAACAA GTCCATGCGA TCACTACGAC
    TGTGCCAACG GTGCACAGTG TGTTGTGAAG GACACGGACC CCGTGTGTCA GTGTCTGCCA
    GGTTATGAGG GTGTGCACTG TGAAAGACTG GTCAGCGTCA ACTTCATCAA CAGAGAATCC
    TTCCTGCAAA TCCCCTCCAA CCTCATTACA GAACAGGCCA ATATATCTCT GCAGATCGCC
    ACAGATGAGG ATAATGGAGT GTTGCTGTAC AAAGGAGATA ATGAACACAT TGCTGTGGAG
    CTTTACAGAG GCCGACTGAG AGTCAGTTAT GACTCTGGAT CATATCCTCC ATCTGCAATC
    TACAGTGTCG AGACAATAAA TGATGGCAGC TTTCATGTAG TGGAGCTGGT GGCAAAGGAT
    CAGTCTCTGT CACTGTCCAT TGATGGAGGA AGCCCGAAAT CCATCAACAC TGCCAATAGC
    CCCAGCCCAG TGCCCAGCCC GGCCCCGCTT TATCTTGGTG GTCTTCCTCA GCAGTCTGGA
    TTGGCCTCGT TGCGTCAGGG CTCTGGCAGG AATGGCTCTA GTTTCCATGG CTGCATCCGA
    AATCTCTACA TTAATGACCA ACTCCAGGAT CTCACCCAGT TTTTGCTGCA GGAGGGTGTT
    GTGCCCGGAT GCCAGCCATG CCAGCGGAGC ATGTGTGCTC ATGGCCAGTG CCATGCCACT
    GGACAGTCCT CTTTCAGCTG TGAGTGTGAG GCGGGATGGA CTGGCCCGCT CTGTGACCAG
    CAGGTCAACA ACCCATGTGA CGGGAACAAG TGTATCCATG GCTCGTGTAT GGCCATAAAC
    TCATACTCGT ACAGCTGCCG TTGTCTTCCA GGCTTTGCAG GTGTGCTGTG TGATGAAGAG
    GAGCAGCTCA GCCCCTGTCA GTATATAGCA TGCAAATACG GCCGATGTCG TGTGTCTGGA
    CTGGGCAAAG CCTATTGTGA GTGCAACAGC GGCTACACGG GGCAAAGCTG CGACAGAGAG
    ATGGCGTGTC GGGGGGAACG GGTCCGAGAT CACTATCAGA CGCAGCAGGG CTACGCCGCG
    TGCCAGAGCA CAGAGAAGGT CTCGCGTTTA GAGTGTCGGG GCAGCTGTGG GGACGGGACC
    TCCTGCTGCG CCCCGCTCCG CAGCAAACGC AGGAAATACA CCTTCCAATG CACAGACGGG
    TCCTCTTTTG TACAGGAGGT GGAAAAGGTT GTCAAGTGTG GCTGCACAAA ATGTCCATCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147305
    Clone ID Related Accession (Same CDS sequence) XM_021475861.1
    Accession Version XM_021475861.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGGCGTAGA AGACTACCGC
    TCAAAACTAG GTGGGGATTG CTTTGCTGAT TTGGCCTGTC CAGAGAAATG TCGATGTGAA
    GGAACCACAG TGGACTGCTC TGGACAAAAG CTCACCAAGA TCCCAGACCA CATCCCTCAG
    TATACAGCAG AACTCCGCTT GAATAATAAT GAATTCACAG TGCTGGAGGC TACAGGAATC
    TTCAAGAAGC TTCCTCAGTT GCGCAAAATC AATCTTAGCA ACAATAAGAT CACAGATATC
    GAAGAAGGCA CATTTGAAGG GGCCAGTGGA GTAAATGAAC TCATACTGAC CAGCAATCGC
    TTGGAGGGGG TTCACTACAG CATGCTGAAA GGCCTCGGTG GTCTCCGCAC ACTGATGTTG
    AGGAGTAACA GGATAAGCTG TGTCAATAAC GGCAGTTTTA CCGGTCTGAG CTCTGTGAGG
    TTGCTTTCCC TCTATGATAA CCTGATCACC TCCATGTCCC CAGGAGCCTT TGACACACTA
    CATTCGCTCT CCACATTGAA CTTGTTGGCC AATCCGTTTA ACTGTAACTG CCATCTGGCC
    TGGCTTGGTG AATGGCTCCG CAAGAAACGG ATTGTCACAG GCAACCCCCG CTGTCAGAGC
    CCCTACTTTC TCAAGGAGAT CCCTATTCAA GATGTAGCTG TACAAGACTT TGCCTGTGAA
    GAAGGTAACG ATGAGAATAG CTGTTCACCA CTGGCTCGCT GTCCTGCCGA GTGTTCGTGT
    TTGGATACAG TGGTTCGTTG CAGTAATAAA GGACTCAAGG TTTTGCCTAA GGGCATTCCT
    AGAGACGTTA CAGAGCTCTA TCTCGATGGC AACGAGTTCA CACAAGTCCC TCTGGAACTA
    TCCAATTATA AACACCTCAC TCTCATTGAT TTGAGTAATA ACCAGATCAG CACATTGTCC
    AACCACAGCT TCAGTAACAT GTCCGAGCTG CTCACACTAA TTTTAAGCTA CAACAGATTA
    AGGTGTATTC CTGCAAAAGC GTTCGACGGC CTCAAGTCTC TGCGCTTGTT GTCTCTCCAT
    GGTAATGATA TTGCTGTGAT CCCAGATGGA GCTTTCAAAG ATCTGTCCTC ACTCTCTCAC
    CTGGCTCTGG GCGCAAACCC CCTGTACTGT GACTGTCACA TGCAGTGGCT CTCTGACTGG
    GTAAAGAGTG GATACAAGGA GCCTGGCATC GCCCGCTGTA CCGGTCCTGG AGACATGACT
    GACAAACTGC TGCTCACTAC ACCCTCTAAG AAATTCACAT GCACAGGTCC AGTGGATGTA
    AGCATTTTGG CTAAATGTAA CCCTTGCTTG TCCAATCCCT GCAAGAATGA TGGGACCTGC
    AGTAACCATC CTGTAGATTT TTACAGATGC ACCTGCCCGT ATGGTTTTAA GGGTCAAGAC
    TGTGAGGAAC CAATTCATGC ATGCATCAGT AACCCCTGTC AGAATGGAGG AACCTGTCAT
    CTGAAGGATG GAGAGGAAAA CACTCACTGG TGTGTGTGTC CAGAAGGATT TGAGGGTGAT
    GAATGTGAGA TAAACATTGA CGACTGTGAG GATAATGACT GTGAGAATAA CTCTACATGC
    GTGGACGGTA TCAACAACTA TACCTGTCTC TGCTCACCTG AATACACAGG GGAGCTGTGT
    GAAGATAAGC TGGATTTTTG TGCTTCAGAG CTTAACCTGT GTCAGCATGA CTCCAAATGT
    ATTCTGACTG CTAAAGGCTT CATGTGTGAA TGCACTCCAG GTTATACCGG TGAGCACTGT
    GAGGTGGATT TTGATGATTG TGAAGATAAT AAGTGTAAAA ACGGGGCTCA GTGTATTGAT
    GCTGTGAACG GCTACACGTG TGTGTGTCCA GAAGGCTACA GTGGGTTGTT CTGCGAGTTT
    TCTCCTCCGA TGGTCCTGCC AAGAACAAGT CCATGCGATC ACTACGACTG TGCCAACGGT
    GCACAGTGTG TTGTGAAGGA CACGGACCCC GTGTGTCAGT GTCTGCCAGG TTATGAGGGT
    GTGCACTGTG AAAGACTGGT CAGCGTCAAC TTCATCAACA GAGAATCCTT CCTGCAAATC
    CCCTCCAACC TCATTACAGA ACAGGCCAAT ATATCTCTGC AGATCGCCAC AGATGAGGAT
    AATGGAGTGT TGCTGTACAA AGGAGATAAT GAACACATTG CTGTGGAGCT TTACAGAGGC
    CGACTGAGAG TCAGTTATGA CTCTGGATCA TATCCTCCAT CTGCAATCTA CAGTGTCGAG
    ACAATAAATG ATGGCAGCTT TCATGTAGTG GAGCTGGTGG CAAAGGATCA GTCTCTGTCA
    CTGTCCATTG ATGGAGGAAG CCCGAAATCC ATCAACACTG CCAATAGCCC CAGCCCAGTG
    CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT CTTCCTCAGC AGTCTGGATT GGCCTCGTTG
    CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT TTCCATGGCT GCATCCGAAA TCTCTACATT
    AATGACCAAC TCCAGGATCT CACCCAGTTT TTGCTGCAGG AGGGTGTTGT GCCCGGATGC
    CAGCCATGCC AGCGGAGCAT GTGTGCTCAT GGCCAGTGCC ATGCCACTGG ACAGTCCTCT
    TTCAGCTGTG AGTGTGAGGC GGGATGGACT GGCCCGCTCT GTGACCAGCA GGTCAACAAC
    CCATGTGACG GGAACAAGTG TATCCATGGC TCGTGTATGG CCATAAACTC ATACTCGTAC
    AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC
    CCCTGTCAGT ATATAGCATG CAAATACGGC CGATGTCGTG TGTCTGGACT GGGCAAAGCC
    TATTGTGAGT GCAACAGCGG CTACACGGGG CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG
    GGGGAACGGG TCCGAGATCA CTATCAGACG CAGCAGGGCT ACGCCGCGTG CCAGAGCACA
    GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC AGCTGTGGGG ACGGGACCTC CTGCTGCGCC
    CCGCTCCGCA GCAAACGCAG GAAATACACC TTCCAATGCA CAGACGGGTC CTCTTTTGTA
    CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331536.1
    CDS827..5377
    Translation

    Target ORF information:

    RefSeq Version XM_021475861.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGGCGTAGA AGACTACCGC
    TCAAAACTAG GTGGGGATTG CTTTGCTGAT TTGGCCTGTC CAGAGAAATG TCGATGTGAA
    GGAACCACAG TGGACTGCTC TGGACAAAAG CTCACCAAGA TCCCAGACCA CATCCCTCAG
    TATACAGCAG AACTCCGCTT GAATAATAAT GAATTCACAG TGCTGGAGGC TACAGGAATC
    TTCAAGAAGC TTCCTCAGTT GCGCAAAATC AATCTTAGCA ACAATAAGAT CACAGATATC
    GAAGAAGGCA CATTTGAAGG GGCCAGTGGA GTAAATGAAC TCATACTGAC CAGCAATCGC
    TTGGAGGGGG TTCACTACAG CATGCTGAAA GGCCTCGGTG GTCTCCGCAC ACTGATGTTG
    AGGAGTAACA GGATAAGCTG TGTCAATAAC GGCAGTTTTA CCGGTCTGAG CTCTGTGAGG
    TTGCTTTCCC TCTATGATAA CCTGATCACC TCCATGTCCC CAGGAGCCTT TGACACACTA
    CATTCGCTCT CCACATTGAA CTTGTTGGCC AATCCGTTTA ACTGTAACTG CCATCTGGCC
    TGGCTTGGTG AATGGCTCCG CAAGAAACGG ATTGTCACAG GCAACCCCCG CTGTCAGAGC
    CCCTACTTTC TCAAGGAGAT CCCTATTCAA GATGTAGCTG TACAAGACTT TGCCTGTGAA
    GAAGGTAACG ATGAGAATAG CTGTTCACCA CTGGCTCGCT GTCCTGCCGA GTGTTCGTGT
    TTGGATACAG TGGTTCGTTG CAGTAATAAA GGACTCAAGG TTTTGCCTAA GGGCATTCCT
    AGAGACGTTA CAGAGCTCTA TCTCGATGGC AACGAGTTCA CACAAGTCCC TCTGGAACTA
    TCCAATTATA AACACCTCAC TCTCATTGAT TTGAGTAATA ACCAGATCAG CACATTGTCC
    AACCACAGCT TCAGTAACAT GTCCGAGCTG CTCACACTAA TTTTAAGCTA CAACAGATTA
    AGGTGTATTC CTGCAAAAGC GTTCGACGGC CTCAAGTCTC TGCGCTTGTT GTCTCTCCAT
    GGTAATGATA TTGCTGTGAT CCCAGATGGA GCTTTCAAAG ATCTGTCCTC ACTCTCTCAC
    CTGGCTCTGG GCGCAAACCC CCTGTACTGT GACTGTCACA TGCAGTGGCT CTCTGACTGG
    GTAAAGAGTG GATACAAGGA GCCTGGCATC GCCCGCTGTA CCGGTCCTGG AGACATGACT
    GACAAACTGC TGCTCACTAC ACCCTCTAAG AAATTCACAT GCACAGGTCC AGTGGATGTA
    AGCATTTTGG CTAAATGTAA CCCTTGCTTG TCCAATCCCT GCAAGAATGA TGGGACCTGC
    AGTAACCATC CTGTAGATTT TTACAGATGC ACCTGCCCGT ATGGTTTTAA GGGTCAAGAC
    TGTGAGGAAC CAATTCATGC ATGCATCAGT AACCCCTGTC AGAATGGAGG AACCTGTCAT
    CTGAAGGATG GAGAGGAAAA CACTCACTGG TGTGTGTGTC CAGAAGGATT TGAGGGTGAT
    GAATGTGAGA TAAACATTGA CGACTGTGAG GATAATGACT GTGAGAATAA CTCTACATGC
    GTGGACGGTA TCAACAACTA TACCTGTCTC TGCTCACCTG AATACACAGG GGAGCTGTGT
    GAAGATAAGC TGGATTTTTG TGCTTCAGAG CTTAACCTGT GTCAGCATGA CTCCAAATGT
    ATTCTGACTG CTAAAGGCTT CATGTGTGAA TGCACTCCAG GTTATACCGG TGAGCACTGT
    GAGGTGGATT TTGATGATTG TGAAGATAAT AAGTGTAAAA ACGGGGCTCA GTGTATTGAT
    GCTGTGAACG GCTACACGTG TGTGTGTCCA GAAGGCTACA GTGGGTTGTT CTGCGAGTTT
    TCTCCTCCGA TGGTCCTGCC AAGAACAAGT CCATGCGATC ACTACGACTG TGCCAACGGT
    GCACAGTGTG TTGTGAAGGA CACGGACCCC GTGTGTCAGT GTCTGCCAGG TTATGAGGGT
    GTGCACTGTG AAAGACTGGT CAGCGTCAAC TTCATCAACA GAGAATCCTT CCTGCAAATC
    CCCTCCAACC TCATTACAGA ACAGGCCAAT ATATCTCTGC AGATCGCCAC AGATGAGGAT
    AATGGAGTGT TGCTGTACAA AGGAGATAAT GAACACATTG CTGTGGAGCT TTACAGAGGC
    CGACTGAGAG TCAGTTATGA CTCTGGATCA TATCCTCCAT CTGCAATCTA CAGTGTCGAG
    ACAATAAATG ATGGCAGCTT TCATGTAGTG GAGCTGGTGG CAAAGGATCA GTCTCTGTCA
    CTGTCCATTG ATGGAGGAAG CCCGAAATCC ATCAACACTG CCAATAGCCC CAGCCCAGTG
    CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT CTTCCTCAGC AGTCTGGATT GGCCTCGTTG
    CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT TTCCATGGCT GCATCCGAAA TCTCTACATT
    AATGACCAAC TCCAGGATCT CACCCAGTTT TTGCTGCAGG AGGGTGTTGT GCCCGGATGC
    CAGCCATGCC AGCGGAGCAT GTGTGCTCAT GGCCAGTGCC ATGCCACTGG ACAGTCCTCT
    TTCAGCTGTG AGTGTGAGGC GGGATGGACT GGCCCGCTCT GTGACCAGCA GGTCAACAAC
    CCATGTGACG GGAACAAGTG TATCCATGGC TCGTGTATGG CCATAAACTC ATACTCGTAC
    AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC
    CCCTGTCAGT ATATAGCATG CAAATACGGC CGATGTCGTG TGTCTGGACT GGGCAAAGCC
    TATTGTGAGT GCAACAGCGG CTACACGGGG CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG
    GGGGAACGGG TCCGAGATCA CTATCAGACG CAGCAGGGCT ACGCCGCGTG CCAGAGCACA
    GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC AGCTGTGGGG ACGGGACCTC CTGCTGCGCC
    CCGCTCCGCA GCAAACGCAG GAAATACACC TTCCAATGCA CAGACGGGTC CTCTTTTGTA
    CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147306
    Clone ID Related Accession (Same CDS sequence) XM_021475862.1
    Accession Version XM_021475862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGATTTG AGGGTGATGA ATGTGAGATA AACATTGACG ACTGTGAGGA TAATGACTGT
    GAGAATAACT CTACATGCGT GGACGGTATC AACAACTATA CCTGTCTCTG CTCACCTGAA
    TACACAGCCG CTAATAATAA TGAAGTGGAG AAAGGGGAGC TGTGTGAAGA TAAGCTGGAT
    TTTTGTGCTT CAGAGCTTAA CCTGTGTCAG CATGACTCCA AATGTATTCT GACTGCTAAA
    GGCTTCATGT GTGAATGCAC TCCAGGTTAT ACCGGTGAGC ACTGTGAGGT GGATTTTGAT
    GATTGTGAAG ATAATAAGTG TAAAAACGGG GCTCAGTGTA TTGATGCTGT GAACGGCTAC
    ACGTGTGTGT GTCCAGAAGG CTACAGTGGG TTGTTCTGCG AGTTTTCTCC TCCGATGGTC
    CTGCCAAGAA CAAGTCCATG CGATCACTAC GACTGTGCCA ACGGTGCACA GTGTGTTGTG
    AAGGACACGG ACCCCGTGTG TCAGTGTCTG CCAGGTTATG AGGGTGTGCA CTGTGAAAGA
    CTGGTCAGCG TCAACTTCAT CAACAGAGAA TCCTTCCTGC AAATCCCCTC CAACCTCATT
    ACAGAACAGG CCAATATATC TCTGCAGATC GCCACAGATG AGGATAATGG AGTGTTGCTG
    TACAAAGGAG ATAATGAACA CATTGCTGTG GAGCTTTACA GAGGCCGACT GAGAGTCAGT
    TATGACTCTG GATCATATCC TCCATCTGCA ATCTACAGTG TCGAGACAAT AAATGATGGC
    AGCTTTCATG TAGTGGAGCT GGTGGCAAAG GATCAGTCTC TGTCACTGTC CATTGATGGA
    GGAAGCCCGA AATCCATCAA CACTGCCAAT AGCCCCAGCC CAGTGCCCAG CCCGGCCCCG
    CTTTATCTTG GTGGTCTTCC TCAGCAGTCT GGATTGGCCT CGTTGCGTCA GGGCTCTGGC
    AGGAATGGCT CTAGTTTCCA TGGCTGCATC CGAAATCTCT ACATTAATGA CCAACTCCAG
    GATCTCACCC AGTTTTTGCT GCAGGAGGGT GTTGTGCCCG GATGCCAGCC ATGCCAGCGG
    AGCATGTGTG CTCATGGCCA GTGCCATGCC ACTGGACAGT CCTCTTTCAG CTGTGAGTGT
    GAGGCGGGAT GGACTGGCCC GCTCTGTGAC CAGCAGGTCA ACAACCCATG TGACGGGAAC
    AAGTGTATCC ATGGCTCGTG TATGGCCATA AACTCATACT CGTACAGCTG CCGTTGTCTT
    CCAGGCTTTG CAGGTGTGCT GTGTGATGAA GAGGAGCAGC TCAGCCCCTG TCAGTATATA
    GCATGCAAAT ACGGCCGATG TCGTGTGTCT GGACTGGGCA AAGCCTATTG TGAGTGCAAC
    AGCGGCTACA CGGGGCAAAG CTGCGACAGA GCTGCTACTG AAAGCGGTAT GGACCCCACT
    CAGGTCCGCC AGAGATGGCG TGTCGGGGGG AACGGGTCCG AGATCACTAT CAGACGCAGC
    AGGGCTACGC CGCGTGCCAG AGCACAGAGA AGGTCTCGCG TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331537.1
    CDS832..5376
    Translation

    Target ORF information:

    RefSeq Version XM_021475862.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGACTTCA GTGAAGGGCC CCAGTCACAC
    TCATCCTGTA GTGTGCTGCA GTGTCCTGAG CTCTGCACCT GCAGTAATAA TGTAGTGGAC
    TGCCGGGGGA AAGGACTTAC TGAAATACCC ACCAACCTGC CAGAAACTAT CACTGAGATT
    CGACTGGAGC AAAACTCCAT CAAGATTATT CCAGCAGGAG CTTTTGCACC TTACAAGCGA
    CTCCGAAGAA TAGATCTAAG TAATAATCAA ATAACTGAAT TGGCTTCAGA CTCCTTTCAA
    GGTCTCCGTT CTTTAAACTC TCTGGTTTTA TATGGGAATA AAATCACTGA ACTTCCCAAA
    GGCCTTTTTG ATGGACTGTT CTCATTGCAG CTACTATTGC TGAATGCGAA CAAGATCAAT
    TGTTTGCGTG TAGATTCTTT CCAAGACCTG CAGAATCTGA ATCTTCTGTC GCTTTATGAC
    AACAAACTTC AGACCATCGC AAAGGGAACT TTCTCTTCTC TCAGAGCTAT CCAAACCCTT
    CATCTGGCCC AGAACCCCTT CATGTGTGAC TGCCATTTGA AGTGGTTAGC AGATTACCTG
    CAAGACAACC CTATTGAGAC CAGTGGAGCT CGGTGCACCA GTCCCCGGCG CCTTGCTAAC
    AAACGTATTG GCCAGATCAA GAGCAAAAAG TTCCGCTGTT CAGCTAAAGA GCAATATTTT
    ATACCAGGGA TTCATCATAT CAATAAGTGC GTAGAAGACT ACCGCTCAAA ACTAGGTGGG
    GATTGCTTTG CTGATTTGGC CTGTCCAGAG AAATGTCGAT GTGAAGGAAC CACAGTGGAC
    TGCTCTGGAC AAAAGCTCAC CAAGATCCCA GACCACATCC CTCAGTATAC AGCAGAACTC
    CGCTTGAATA ATAATGAATT CACAGTGCTG GAGGCTACAG GAATCTTCAA GAAGCTTCCT
    CAGTTGCGCA AAATCAATCT TAGCAACAAT AAGATCACAG ATATCGAAGA AGGCACATTT
    GAAGGGGCCA GTGGAGTAAA TGAACTCATA CTGACCAGCA ATCGCTTGGA GGGGGTTCAC
    TACAGCATGC TGAAAGGCCT CGGTGGTCTC CGCACACTGA TGTTGAGGAG TAACAGGATA
    AGCTGTGTCA ATAACGGCAG TTTTACCGGT CTGAGCTCTG TGAGGTTGCT TTCCCTCTAT
    GATAACCTGA TCACCTCCAT GTCCCCAGGA GCCTTTGACA CACTACATTC GCTCTCCACA
    TTGAACTTGT TGGCCAATCC GTTTAACTGT AACTGCCATC TGGCCTGGCT TGGTGAATGG
    CTCCGCAAGA AACGGATTGT CACAGGCAAC CCCCGCTGTC AGAGCCCCTA CTTTCTCAAG
    GAGATCCCTA TTCAAGATGT AGCTGTACAA GACTTTGCCT GTGAAGAAGG TAACGATGAG
    AATAGCTGTT CACCACTGGC TCGCTGTCCT GCCGAGTGTT CGTGTTTGGA TACAGTGGTT
    CGTTGCAGTA ATAAAGGACT CAAGGTTTTG CCTAAGGGCA TTCCTAGAGA CGTTACAGAG
    CTCTATCTCG ATGGCAACGA GTTCACACAA GTCCCTCTGG AACTATCCAA TTATAAACAC
    CTCACTCTCA TTGATTTGAG TAATAACCAG ATCAGCACAT TGTCCAACCA CAGCTTCAGT
    AACATGTCCG AGCTGCTCAC ACTAATTTTA AGCTACAACA GATTAAGGTG TATTCCTGCA
    AAAGCGTTCG ACGGCCTCAA GTCTCTGCGC TTGTTGTCTC TCCATGGTAA TGATATTGCT
    GTGATCCCAG ATGGAGCTTT CAAAGATCTG TCCTCACTCT CTCACCTGGC TCTGGGCGCA
    AACCCCCTGT ACTGTGACTG TCACATGCAG TGGCTCTCTG ACTGGGTAAA GAGTGGATAC
    AAGGAGCCTG GCATCGCCCG CTGTACCGGT CCTGGAGACA TGACTGACAA ACTGCTGCTC
    ACTACACCCT CTAAGAAATT CACATGCACA GAGGTGCCGG GGACTTGTGG TTTTCCTGTG
    CCAGGTCCAG TGGATGTAAG CATTTTGGCT AAATGTAACC CTTGCTTGTC CAATCCCTGC
    AAGAATGATG GGACCTGCAG TAACCATCCT GTAGATTTTT ACAGATGCAC CTGCCCGTAT
    GGTTTTAAGG GTCAAGACTG TGAGGAACCA ATTCATGCAT GCATCAGTAA CCCCTGTCAG
    AATGGAGGAA CCTGTCATCT GAAGGATGGA GAGGAAAACA CTCACTGGTG TGTGTGTCCA
    GAAGGATTTG AGGGTGATGA ATGTGAGATA AACATTGACG ACTGTGAGGA TAATGACTGT
    GAGAATAACT CTACATGCGT GGACGGTATC AACAACTATA CCTGTCTCTG CTCACCTGAA
    TACACAGCCG CTAATAATAA TGAAGTGGAG AAAGGGGAGC TGTGTGAAGA TAAGCTGGAT
    TTTTGTGCTT CAGAGCTTAA CCTGTGTCAG CATGACTCCA AATGTATTCT GACTGCTAAA
    GGCTTCATGT GTGAATGCAC TCCAGGTTAT ACCGGTGAGC ACTGTGAGGT GGATTTTGAT
    GATTGTGAAG ATAATAAGTG TAAAAACGGG GCTCAGTGTA TTGATGCTGT GAACGGCTAC
    ACGTGTGTGT GTCCAGAAGG CTACAGTGGG TTGTTCTGCG AGTTTTCTCC TCCGATGGTC
    CTGCCAAGAA CAAGTCCATG CGATCACTAC GACTGTGCCA ACGGTGCACA GTGTGTTGTG
    AAGGACACGG ACCCCGTGTG TCAGTGTCTG CCAGGTTATG AGGGTGTGCA CTGTGAAAGA
    CTGGTCAGCG TCAACTTCAT CAACAGAGAA TCCTTCCTGC AAATCCCCTC CAACCTCATT
    ACAGAACAGG CCAATATATC TCTGCAGATC GCCACAGATG AGGATAATGG AGTGTTGCTG
    TACAAAGGAG ATAATGAACA CATTGCTGTG GAGCTTTACA GAGGCCGACT GAGAGTCAGT
    TATGACTCTG GATCATATCC TCCATCTGCA ATCTACAGTG TCGAGACAAT AAATGATGGC
    AGCTTTCATG TAGTGGAGCT GGTGGCAAAG GATCAGTCTC TGTCACTGTC CATTGATGGA
    GGAAGCCCGA AATCCATCAA CACTGCCAAT AGCCCCAGCC CAGTGCCCAG CCCGGCCCCG
    CTTTATCTTG GTGGTCTTCC TCAGCAGTCT GGATTGGCCT CGTTGCGTCA GGGCTCTGGC
    AGGAATGGCT CTAGTTTCCA TGGCTGCATC CGAAATCTCT ACATTAATGA CCAACTCCAG
    GATCTCACCC AGTTTTTGCT GCAGGAGGGT GTTGTGCCCG GATGCCAGCC ATGCCAGCGG
    AGCATGTGTG CTCATGGCCA GTGCCATGCC ACTGGACAGT CCTCTTTCAG CTGTGAGTGT
    GAGGCGGGAT GGACTGGCCC GCTCTGTGAC CAGCAGGTCA ACAACCCATG TGACGGGAAC
    AAGTGTATCC ATGGCTCGTG TATGGCCATA AACTCATACT CGTACAGCTG CCGTTGTCTT
    CCAGGCTTTG CAGGTGTGCT GTGTGATGAA GAGGAGCAGC TCAGCCCCTG TCAGTATATA
    GCATGCAAAT ACGGCCGATG TCGTGTGTCT GGACTGGGCA AAGCCTATTG TGAGTGCAAC
    AGCGGCTACA CGGGGCAAAG CTGCGACAGA GCTGCTACTG AAAGCGGTAT GGACCCCACT
    CAGGTCCGCC AGAGATGGCG TGTCGGGGGG AACGGGTCCG AGATCACTAT CAGACGCAGC
    AGGGCTACGC CGCGTGCCAG AGCACAGAGA AGGTCTCGCG TTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa03853
    Clone ID Related Accession (Same CDS sequence) NM_131735.1
    Accession Version NM_131735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4539bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Danio rerio(zebrafish)
    Product slit homolog 2 protein precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF210321.1. On Jul 6, 2005 this sequence version replaced XM_696894.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF210321.1, CU638813.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GGGTGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGTAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGAGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGATAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGCTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGGTCCAG TGGATGTAAG CATTTTGGCT
    AAATGTAACC CTTGCTTGTC CAATCCCTGC AAGAATGATG GGACCTGCAG TAACCATCCT
    GTAGATTTTT ACAGATGCAC CTGCCCGTAT GGTTTTAAGG GTCAAGACTG TGAGGAACCA
    ATTCATGCAT GCATCAGTAA CCCCTGTCAG AATGGAGGAA CCTGTCATCT GAAGGATGGA
    GAGGAAAACA CTCACTGGTG TGTGTGTCCA GAAGGATTTG AGGGTGATGA ATGTGAGATA
    AACATTGACG ACTGTGAGGA TAATGACTGT GAGAATAACT CTACATGCGT GGACGGTATC
    AACAACTACA CCTGTCTCTG CTCACCTGAA TACACAGGGG AGCTGTGTGA AGATAAGCTG
    GATTTTTGTG CTTCAGAGCT TAACCTGTGT CAGCATGACT CCAAATGTAT TCTGACTGCT
    AAAGGCTTCA TGTGTGAATG CACTCCAGGT TATACCGGTG AGCACTGTGA GGTGGATTTT
    GATGATTGTG AAGATAATAA GTGTAAAAAC GGGGCTCAGT GTATTGATGC TGTGAACGGC
    TACACGTGTG TGTGTCCAGA AGGCTACAGT GGGTTGTTCT GCGAGTTTTC TCCTCCGATG
    GTCCTGCCAA GAACAAGTCC ATGCGATCAC TACGACTGTG CCAACGGTGC ACAGTGTGTT
    GTGAAGGACA CGGACCCCGT GTGTCAGTGT CTGCCAGGTT ATGAGGGTGT GCACTGTGAA
    AGACTGGTCA GCGTCAACTT CATCAACAGA GAATCCTTCC TGCAAATCCC CTCCAACCTC
    ATTACAGAAC AGGCCAATAT ATCTCTGCAG ATCGCCACAG ATGAGGATAA TGGAGTGTTG
    CTGTACAAAG GAGATAATGA ACACATTGCT GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC
    AGTTATGACT CTGGATCATA TCCTCCATCT GCAATCTACA GTGTCGAGAC AATAAATGAT
    GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA AAGGATCAGT CTCTGTCACT GTCCATTGAT
    GGAGGAAGCC CGAAATCCAT CAACACTGCC AATAGCCCCA GCCCAGTGCC CAGCCCGGCC
    CCGCTTTATC TTGGTGGTCT TCCTCAGCAG TCTGGATTGG CCTCGTTGCG TCAGGGCTCT
    GGCAGGAATG GCTCTAGTTT CCATGGCTGC ATCCGAAATC TCTACATTAA TGACCAACTC
    CAGGATCTCA CCCAGTTTTT GCTGCAGGAG GGTGTTGTGC CCGGATGCCA GCCATGCCAG
    CGGAGCATGT GTGCTCATGG CCAGTGCCAT GCCACTGGAC AGTCCTCTTT CAGCTGTGAG
    TGTGAGGCGG GATGGACTGG CCCGCTCTGT GACCAGCAGG TCAACAACCC ATGTGACGGG
    AACAAGTGTA TCCATGGCTC GTGTATGGCC ATAAACTCAT ACTCGTACAG CTGCCGTTGT
    CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT
    ATAGCATGCA AATACGGCCG ATGTCGTGTG TCTGGACTGG GCAAAGCCTA TTGTGAGTGC
    AACAGCGGCT ACACGGGGCA AAGCTGCGAC AGAGAGATGG CGTGTCGGGG GGAACGGGTC
    CGAGATCACT ATCAGACGCA GCAGGGCTAC GCCGCGTGCC AGAGCACAGA GAAGGTCTCG
    CGTTTAGAGT GTCGGGGCAG CTGTGGGGAC GGGACCTCCT GCTGCGCCCC GCTCCGCAGC
    AAACGCAGGA AATACACCTT CCAATGCACA GACGGGTCCT CTTTTGTACA GGAGGTGGAA
    AAGGTTGTCA AGTGTGGCTG CACAAAATGT CCATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571810.1
    CDS1..4539
    Translation

    Target ORF information:

    RefSeq Version NM_131735.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GGGTGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGTAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGAGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGATAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGCTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGGTCCAG TGGATGTAAG CATTTTGGCT
    AAATGTAACC CTTGCTTGTC CAATCCCTGC AAGAATGATG GGACCTGCAG TAACCATCCT
    GTAGATTTTT ACAGATGCAC CTGCCCGTAT GGTTTTAAGG GTCAAGACTG TGAGGAACCA
    ATTCATGCAT GCATCAGTAA CCCCTGTCAG AATGGAGGAA CCTGTCATCT GAAGGATGGA
    GAGGAAAACA CTCACTGGTG TGTGTGTCCA GAAGGATTTG AGGGTGATGA ATGTGAGATA
    AACATTGACG ACTGTGAGGA TAATGACTGT GAGAATAACT CTACATGCGT GGACGGTATC
    AACAACTACA CCTGTCTCTG CTCACCTGAA TACACAGGGG AGCTGTGTGA AGATAAGCTG
    GATTTTTGTG CTTCAGAGCT TAACCTGTGT CAGCATGACT CCAAATGTAT TCTGACTGCT
    AAAGGCTTCA TGTGTGAATG CACTCCAGGT TATACCGGTG AGCACTGTGA GGTGGATTTT
    GATGATTGTG AAGATAATAA GTGTAAAAAC GGGGCTCAGT GTATTGATGC TGTGAACGGC
    TACACGTGTG TGTGTCCAGA AGGCTACAGT GGGTTGTTCT GCGAGTTTTC TCCTCCGATG
    GTCCTGCCAA GAACAAGTCC ATGCGATCAC TACGACTGTG CCAACGGTGC ACAGTGTGTT
    GTGAAGGACA CGGACCCCGT GTGTCAGTGT CTGCCAGGTT ATGAGGGTGT GCACTGTGAA
    AGACTGGTCA GCGTCAACTT CATCAACAGA GAATCCTTCC TGCAAATCCC CTCCAACCTC
    ATTACAGAAC AGGCCAATAT ATCTCTGCAG ATCGCCACAG ATGAGGATAA TGGAGTGTTG
    CTGTACAAAG GAGATAATGA ACACATTGCT GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC
    AGTTATGACT CTGGATCATA TCCTCCATCT GCAATCTACA GTGTCGAGAC AATAAATGAT
    GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA AAGGATCAGT CTCTGTCACT GTCCATTGAT
    GGAGGAAGCC CGAAATCCAT CAACACTGCC AATAGCCCCA GCCCAGTGCC CAGCCCGGCC
    CCGCTTTATC TTGGTGGTCT TCCTCAGCAG TCTGGATTGG CCTCGTTGCG TCAGGGCTCT
    GGCAGGAATG GCTCTAGTTT CCATGGCTGC ATCCGAAATC TCTACATTAA TGACCAACTC
    CAGGATCTCA CCCAGTTTTT GCTGCAGGAG GGTGTTGTGC CCGGATGCCA GCCATGCCAG
    CGGAGCATGT GTGCTCATGG CCAGTGCCAT GCCACTGGAC AGTCCTCTTT CAGCTGTGAG
    TGTGAGGCGG GATGGACTGG CCCGCTCTGT GACCAGCAGG TCAACAACCC ATGTGACGGG
    AACAAGTGTA TCCATGGCTC GTGTATGGCC ATAAACTCAT ACTCGTACAG CTGCCGTTGT
    CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT
    ATAGCATGCA AATACGGCCG ATGTCGTGTG TCTGGACTGG GCAAAGCCTA TTGTGAGTGC
    AACAGCGGCT ACACGGGGCA AAGCTGCGAC AGAGAGATGG CGTGTCGGGG GGAACGGGTC
    CGAGATCACT ATCAGACGCA GCAGGGCTAC GCCGCGTGCC AGAGCACAGA GAAGGTCTCG
    CGTTTAGAGT GTCGGGGCAG CTGTGGGGAC GGGACCTCCT GCTGCGCCCC GCTCCGCAGC
    AAACGCAGGA AATACACCTT CCAATGCACA GACGGGTCCT CTTTTGTACA GGAGGTGGAA
    AAGGTTGTCA AGTGTGGCTG CACAAAATGT CCATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39428
    Clone ID Related Accession (Same CDS sequence) XM_009291085.2
    Accession Version XM_009291085.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4467bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product slit homolog 2 protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATAATTTT AAGCTACAAC AGATTAAGGT GTATTCCTGC AAAAGCGTTC
    GACGGCCTCA AGTCTCTGCG CTTGTTGTCT CTCCATGGTA ATGATATTGC TGTGATCCCA
    GATGGAGCTT TCAAAGATCT GTCCTCACTC TCTCACCTGG CTCTGGGCGC AAACCCCCTG
    TACTGTGACT GTCACATGCA GTGGCTCTCT GACTGGGTAA AGAGTGGATA CAAGGAGCCT
    GGCATCGCCC GCTGTACCGG TCCTGGAGAC ATGACTGACA AACTGCTGCT CACTACACCC
    TCTAAGAAAT TCACATGCAC AGGTCCAGTG GATGTAAGCA TTTTGGCTAA ATGTAACCCT
    TGCTTGTCCA ATCCCTGCAA GAATGATGGG ACCTGCAGTA ACCATCCTGT AGATTTTTAC
    AGATGCACCT GCCCGTATGG TTTTAAGGGT CAAGACTGTG AGGAACCAAT TCATGCATGC
    ATCAGTAACC CCTGTCAGAA TGGAGGAACC TGTCATCTGA AGGATGGAGA GGAAAACACT
    CACTGGTGTG TGTGTCCAGA AGGATTTGAG GGTGATGAAT GTGAGATAAA CATTGACGAC
    TGTGAGGATA ATGACTGTGA GAATAACTCT ACATGCGTGG ACGGTATCAA CAACTATACC
    TGTCTCTGCT CACCTGAATA CACAGGGGAG CTGTGTGAAG ATAAGCTGGA TTTTTGTGCT
    TCAGAGCTTA ACCTGTGTCA GCATGACTCC AAATGTATTC TGACTGCTAA AGGCTTCATG
    TGTGAATGCA CTCCAGGTTA TACCGGTGAG CACTGTGAGG TGGATTTTGA TGATTGTGAA
    GATAATAAGT GTAAAAACGG GGCTCAGTGT ATTGATGCTG TGAACGGCTA CACGTGTGTG
    TGTCCAGAAG GCTACAGTGG GTTGTTCTGC GAGTTTTCTC CTCCGATGGT CCTGCCAAGA
    ACAAGTCCAT GCGATCACTA CGACTGTGCC AACGGTGCAC AGTGTGTTGT GAAGGACACG
    GACCCCGTGT GTCAGTGTCT GCCAGGTTAT GAGGGTGTGC ACTGTGAAAG ACTGGTCAGC
    GTCAACTTCA TCAACAGAGA ATCCTTCCTG CAAATCCCCT CCAACCTCAT TACAGAACAG
    GCCAATATAT CTCTGCAGAT CGCCACAGAT GAGGATAATG GAGTGTTGCT GTACAAAGGA
    GATAATGAAC ACATTGCTGT GGAGCTTTAC AGAGGCCGAC TGAGAGTCAG TTATGACTCT
    GGATCATATC CTCCATCTGC AATCTACAGT GTCGAGACAA TAAATGATGG CAGCTTTCAT
    GTAGTGGAGC TGGTGGCAAA GGATCAGTCT CTGTCACTGT CCATTGATGG AGGAAGCCCG
    AAATCCATCA ACACTGCCAA TAGCCCCAGC CCAGTGCCCA GCCCGGCCCC GCTTTATCTT
    GGTGGTCTTC CTCAGCAGTC TGGATTGGCC TCGTTGCGTC AGGGCTCTGG CAGGAATGGC
    TCTAGTTTCC ATGGCTGCAT CCGAAATCTC TACATTAATG ACCAACTCCA GGATCTCACC
    CAGTTTTTGC TGCAGGAGGG TGTTGTGCCC GGATGCCAGC CATGCCAGCG GAGCATGTGT
    GCTCATGGCC AGTGCCATGC CACTGGACAG TCCTCTTTCA GCTGTGAGTG TGAGGCGGGA
    TGGACTGGCC CGCTCTGTGA CCAGCAGGTC AACAACCCAT GTGACGGGAA CAAGTGTATC
    CATGGCTCGT GTATGGCCAT AAACTCATAC TCGTACAGCT GCCGTTGTCT TCCAGGCTTT
    GCAGGTGTGC TGTGTGATGA AGAGGAGCAG CTCAGCCCCT GTCAGTATAT AGCATGCAAA
    TACGGCCGAT GTCGTGTGTC TGGACTGGGC AAAGCCTATT GTGAGTGCAA CAGCGGCTAC
    ACGGGGCAAA GCTGCGACAG AGAGATGGCG TGTCGGGGGG AACGGGTCCG AGATCACTAT
    CAGACGCAGC AGGGCTACGC CGCGTGCCAG AGCACAGAGA AGGTCTCGCG TTTAGAGTGT
    CGGGGCAGCT GTGGGGACGG GACCTCCTGC TGCGCCCCGC TCCGCAGCAA ACGCAGGAAA
    TACACCTTCC AATGCACAGA CGGGTCCTCT TTTGTACAGG AGGTGGAAAA GGTTGTCAAG
    TGTGGCTGCA CAAAATGTCC ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289360.1
    CDS1184..5650
    Translation

    Target ORF information:

    RefSeq Version XM_009291085.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291085.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATAATTTT AAGCTACAAC AGATTAAGGT GTATTCCTGC AAAAGCGTTC
    GACGGCCTCA AGTCTCTGCG CTTGTTGTCT CTCCATGGTA ATGATATTGC TGTGATCCCA
    GATGGAGCTT TCAAAGATCT GTCCTCACTC TCTCACCTGG CTCTGGGCGC AAACCCCCTG
    TACTGTGACT GTCACATGCA GTGGCTCTCT GACTGGGTAA AGAGTGGATA CAAGGAGCCT
    GGCATCGCCC GCTGTACCGG TCCTGGAGAC ATGACTGACA AACTGCTGCT CACTACACCC
    TCTAAGAAAT TCACATGCAC AGGTCCAGTG GATGTAAGCA TTTTGGCTAA ATGTAACCCT
    TGCTTGTCCA ATCCCTGCAA GAATGATGGG ACCTGCAGTA ACCATCCTGT AGATTTTTAC
    AGATGCACCT GCCCGTATGG TTTTAAGGGT CAAGACTGTG AGGAACCAAT TCATGCATGC
    ATCAGTAACC CCTGTCAGAA TGGAGGAACC TGTCATCTGA AGGATGGAGA GGAAAACACT
    CACTGGTGTG TGTGTCCAGA AGGATTTGAG GGTGATGAAT GTGAGATAAA CATTGACGAC
    TGTGAGGATA ATGACTGTGA GAATAACTCT ACATGCGTGG ACGGTATCAA CAACTATACC
    TGTCTCTGCT CACCTGAATA CACAGGGGAG CTGTGTGAAG ATAAGCTGGA TTTTTGTGCT
    TCAGAGCTTA ACCTGTGTCA GCATGACTCC AAATGTATTC TGACTGCTAA AGGCTTCATG
    TGTGAATGCA CTCCAGGTTA TACCGGTGAG CACTGTGAGG TGGATTTTGA TGATTGTGAA
    GATAATAAGT GTAAAAACGG GGCTCAGTGT ATTGATGCTG TGAACGGCTA CACGTGTGTG
    TGTCCAGAAG GCTACAGTGG GTTGTTCTGC GAGTTTTCTC CTCCGATGGT CCTGCCAAGA
    ACAAGTCCAT GCGATCACTA CGACTGTGCC AACGGTGCAC AGTGTGTTGT GAAGGACACG
    GACCCCGTGT GTCAGTGTCT GCCAGGTTAT GAGGGTGTGC ACTGTGAAAG ACTGGTCAGC
    GTCAACTTCA TCAACAGAGA ATCCTTCCTG CAAATCCCCT CCAACCTCAT TACAGAACAG
    GCCAATATAT CTCTGCAGAT CGCCACAGAT GAGGATAATG GAGTGTTGCT GTACAAAGGA
    GATAATGAAC ACATTGCTGT GGAGCTTTAC AGAGGCCGAC TGAGAGTCAG TTATGACTCT
    GGATCATATC CTCCATCTGC AATCTACAGT GTCGAGACAA TAAATGATGG CAGCTTTCAT
    GTAGTGGAGC TGGTGGCAAA GGATCAGTCT CTGTCACTGT CCATTGATGG AGGAAGCCCG
    AAATCCATCA ACACTGCCAA TAGCCCCAGC CCAGTGCCCA GCCCGGCCCC GCTTTATCTT
    GGTGGTCTTC CTCAGCAGTC TGGATTGGCC TCGTTGCGTC AGGGCTCTGG CAGGAATGGC
    TCTAGTTTCC ATGGCTGCAT CCGAAATCTC TACATTAATG ACCAACTCCA GGATCTCACC
    CAGTTTTTGC TGCAGGAGGG TGTTGTGCCC GGATGCCAGC CATGCCAGCG GAGCATGTGT
    GCTCATGGCC AGTGCCATGC CACTGGACAG TCCTCTTTCA GCTGTGAGTG TGAGGCGGGA
    TGGACTGGCC CGCTCTGTGA CCAGCAGGTC AACAACCCAT GTGACGGGAA CAAGTGTATC
    CATGGCTCGT GTATGGCCAT AAACTCATAC TCGTACAGCT GCCGTTGTCT TCCAGGCTTT
    GCAGGTGTGC TGTGTGATGA AGAGGAGCAG CTCAGCCCCT GTCAGTATAT AGCATGCAAA
    TACGGCCGAT GTCGTGTGTC TGGACTGGGC AAAGCCTATT GTGAGTGCAA CAGCGGCTAC
    ACGGGGCAAA GCTGCGACAG AGAGATGGCG TGTCGGGGGG AACGGGTCCG AGATCACTAT
    CAGACGCAGC AGGGCTACGC CGCGTGCCAG AGCACAGAGA AGGTCTCGCG TTTAGAGTGT
    CGGGGCAGCT GTGGGGACGG GACCTCCTGC TGCGCCCCGC TCCGCAGCAA ACGCAGGAAA
    TACACCTTCC AATGCACAGA CGGGTCCTCT TTTGTACAGG AGGTGGAAAA GGTTGTCAAG
    TGTGGCTGCA CAAAATGTCC ATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39429
    Clone ID Related Accession (Same CDS sequence) XM_009291088.2
    Accession Version XM_009291088.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4428bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product slit homolog 2 protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGGTCCAG TGGATGTAAG CATTTTGGCT
    AAATGTAACC CTTGCTTGTC CAATCCCTGC AAGAATGATG GGACCTGCAG TAACCATCCT
    GTAGATTTTT ACAGATGCAC CTGCCCGTAT GGTTTTAAGG GTCAAGACTG TGAGGAACCA
    ATTCATGCAT GCATCAGTAA CCCCTGTCAG AATGGAGGAA CCTGTCATCT GAAGGATGGA
    GAGGAAAACA CTCACTGGTG TGTGTGTCCA GAAGGATTTG AGGGTGATGA ATGTGAGATA
    AACATTGACG ACTGTGAGGA TAATGACTGT GAGAATAACT CTACATGCGT GGACGGTATC
    AACAACTATA CCTGTCTCTG CTCACCTGAA TACACAGGGG AGCTGTGTGA AGATAAGCTG
    GATTTTTGTG CTTCAGAGCT TAACCTGTGT CAGCATGACT CCAAATGTAT TCTGACTGCT
    AAAGGCTTCA TGTGTGAATG CACTCCAGGT TATACCGGTG AGCACTGTGA GGTGGATTTT
    GATGATTGTG AAGATAATAA GTGTAAAAAC GGGGCTCAGT GTATTGATGC TGTGAACGGC
    TACACGTGTG TGTGTCCAGA AGGCTACAGT GGGTTGTTCT GCGAGTTTTC TCCTCCGATG
    GTCCTGCCAA GAACAAGTCC ATGCGATCAC TACGACTGTG CCAACGGTGC ACAGTGTGTT
    GTGAAGGACA CGGACCCCGT GTGTCAGTGT CTGCCAGGTT ATGAGGGTGT GCACTGTGAA
    AGACTGGTCA GCGTCAACTT CATCAACAGA GAATCCTTCC TGCAAATCCC CTCCAACCTC
    ATTACAGAAC AGGCCAATAT ATCTCTGCAG ATCGCCACAG ATGAGGATAA TGGAGTGTTG
    CTGTACAAAG GAGATAATGA ACACATTGCT GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC
    AGTTATGACT CTGGATCATA TCCTCCATCT GCAATCTACA GTGTCGAGAC AATAAATGAT
    GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA AAGGATCAGT CTCTGTCACT GTCCATTGAT
    GGAGGAAGCC CGAAATCCAT CAACACTGCC AATAGCCCCA GCCCAGTGCC CAGCCCGGCC
    CCGCTTTATC TTGGTGGTCT TCCTCAGCAG TCTGGATTGG CCTCGTTGCG TCAGGGCTCT
    GGCAGGAATG GCTCTAGTTT CCATGGCTGC ATCCGAAATC TCTACATTAA TGACCAACTC
    CAGGATCTCA CCCAGTTTTT GCTGCAGGAG GGTGTTGTGC CCGGATGCCA GCCATGCCAG
    CGGAGCATGT GTGCTCATGG CCAGTGCCAT GCCACTGGAC AGTCCTCTTT CAGCTGTGAG
    TGTGAGGCGG GATGGACTGG CCCGCTCTGT GACCAGCAGG TCAACAACCC ATGTGACGGG
    AACAAGTGTA TCCATGGCTC GTGTATGGCC ATAAACTCAT ACTCGTACAG CTGCCGTTGT
    CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT
    ATAGCATGCA AATACGGCCG ATGTCGTGTG TCTGGACTGG GCAAAGCCTA TTGTGAGTGC
    AACAGCGGCT ACACGGGGCA AAGCTGCGAC AGAGCTGCTA CTGAAAGCGG TATGGACCCC
    ACTCAGGTCC GCCAGAGATG GCGTGTCGGG GGGAACGGGT CCGAGATCAC TATCAGACGC
    AGCAGGGCTA CGCCGCGTGC CAGAGCACAG AGAAGGTCTC GCGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289363.1
    CDS1187..5614
    Translation

    Target ORF information:

    RefSeq Version XM_009291088.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291088.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGGTCCAG TGGATGTAAG CATTTTGGCT
    AAATGTAACC CTTGCTTGTC CAATCCCTGC AAGAATGATG GGACCTGCAG TAACCATCCT
    GTAGATTTTT ACAGATGCAC CTGCCCGTAT GGTTTTAAGG GTCAAGACTG TGAGGAACCA
    ATTCATGCAT GCATCAGTAA CCCCTGTCAG AATGGAGGAA CCTGTCATCT GAAGGATGGA
    GAGGAAAACA CTCACTGGTG TGTGTGTCCA GAAGGATTTG AGGGTGATGA ATGTGAGATA
    AACATTGACG ACTGTGAGGA TAATGACTGT GAGAATAACT CTACATGCGT GGACGGTATC
    AACAACTATA CCTGTCTCTG CTCACCTGAA TACACAGGGG AGCTGTGTGA AGATAAGCTG
    GATTTTTGTG CTTCAGAGCT TAACCTGTGT CAGCATGACT CCAAATGTAT TCTGACTGCT
    AAAGGCTTCA TGTGTGAATG CACTCCAGGT TATACCGGTG AGCACTGTGA GGTGGATTTT
    GATGATTGTG AAGATAATAA GTGTAAAAAC GGGGCTCAGT GTATTGATGC TGTGAACGGC
    TACACGTGTG TGTGTCCAGA AGGCTACAGT GGGTTGTTCT GCGAGTTTTC TCCTCCGATG
    GTCCTGCCAA GAACAAGTCC ATGCGATCAC TACGACTGTG CCAACGGTGC ACAGTGTGTT
    GTGAAGGACA CGGACCCCGT GTGTCAGTGT CTGCCAGGTT ATGAGGGTGT GCACTGTGAA
    AGACTGGTCA GCGTCAACTT CATCAACAGA GAATCCTTCC TGCAAATCCC CTCCAACCTC
    ATTACAGAAC AGGCCAATAT ATCTCTGCAG ATCGCCACAG ATGAGGATAA TGGAGTGTTG
    CTGTACAAAG GAGATAATGA ACACATTGCT GTGGAGCTTT ACAGAGGCCG ACTGAGAGTC
    AGTTATGACT CTGGATCATA TCCTCCATCT GCAATCTACA GTGTCGAGAC AATAAATGAT
    GGCAGCTTTC ATGTAGTGGA GCTGGTGGCA AAGGATCAGT CTCTGTCACT GTCCATTGAT
    GGAGGAAGCC CGAAATCCAT CAACACTGCC AATAGCCCCA GCCCAGTGCC CAGCCCGGCC
    CCGCTTTATC TTGGTGGTCT TCCTCAGCAG TCTGGATTGG CCTCGTTGCG TCAGGGCTCT
    GGCAGGAATG GCTCTAGTTT CCATGGCTGC ATCCGAAATC TCTACATTAA TGACCAACTC
    CAGGATCTCA CCCAGTTTTT GCTGCAGGAG GGTGTTGTGC CCGGATGCCA GCCATGCCAG
    CGGAGCATGT GTGCTCATGG CCAGTGCCAT GCCACTGGAC AGTCCTCTTT CAGCTGTGAG
    TGTGAGGCGG GATGGACTGG CCCGCTCTGT GACCAGCAGG TCAACAACCC ATGTGACGGG
    AACAAGTGTA TCCATGGCTC GTGTATGGCC ATAAACTCAT ACTCGTACAG CTGCCGTTGT
    CTTCCAGGCT TTGCAGGTGT GCTGTGTGAT GAAGAGGAGC AGCTCAGCCC CTGTCAGTAT
    ATAGCATGCA AATACGGCCG ATGTCGTGTG TCTGGACTGG GCAAAGCCTA TTGTGAGTGC
    AACAGCGGCT ACACGGGGCA AAGCTGCGAC AGAGCTGCTA CTGAAAGCGG TATGGACCCC
    ACTCAGGTCC GCCAGAGATG GCGTGTCGGG GGGAACGGGT CCGAGATCAC TATCAGACGC
    AGCAGGGCTA CGCCGCGTGC CAGAGCACAG AGAAGGTCTC GCGTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39430
    Clone ID Related Accession (Same CDS sequence) XM_009291092.2
    Accession Version XM_009291092.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product slit homolog 2 protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGGTCCAG TGGATGTAAG CATTTTGGCT
    AAATGTAACC CTTGCTTGTC CAATCCCTGC AAGAATGATG GGACCTGCAG TAACCATCCT
    GTAGATTTTT ACAGATGCAC CTGCCCGTAT GGTTTTAAGG GTCAAGACTG TGAGGAACCA
    ATTCATGCAT GCATCAGTAA CCCCTGTCAG AATGGAGGAA CCTGTCATCT GAAGGATGGA
    GAGGAAAACA CTCACTGGTG TGTGTGTCCA GAAGGCTACA GTGGGTTGTT CTGCGAGTTT
    TCTCCTCCGA TGGTCCTGCC AAGAACAAGT CCATGCGATC ACTACGACTG TGCCAACGGT
    GCACAGTGTG TTGTGAAGGA CACGGACCCC GTGTGTCAGT GTCTGCCAGG TTATGAGGGT
    GTGCACTGTG AAAGACTGGT CAGCGTCAAC TTCATCAACA GAGAATCCTT CCTGCAAATC
    CCCTCCAACC TCATTACAGA ACAGGCCAAT ATATCTCTGC AGATCGCCAC AGATGAGGAT
    AATGGAGTGT TGCTGTACAA AGGAGATAAT GAACACATTG CTGTGGAGCT TTACAGAGGC
    CGACTGAGAG TCAGTTATGA CTCTGGATCA TATCCTCCAT CTGCAATCTA CAGTGTCGAG
    ACAATAAATG ATGGCAGCTT TCATGTAGTG GAGCTGGTGG CAAAGGATCA GTCTCTGTCA
    CTGTCCATTG ATGGAGGAAG CCCGAAATCC ATCAACACTG CCAATAGCCC CAGCCCAGTG
    CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT CTTCCTCAGC AGTCTGGATT GGCCTCGTTG
    CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT TTCCATGGCT GCATCCGAAA TCTCTACATT
    AATGACCAAC TCCAGGATCT CACCCAGTTT TTGCTGCAGG AGGGTGTTGT GCCCGGATGC
    CAGCCATGCC AGCGGAGCAT GTGTGCTCAT GGCCAGTGCC ATGCCACTGG ACAGTCCTCT
    TTCAGCTGTG AGTGTGAGGC GGGATGGACT GGCCCGCTCT GTGACCAGCA GGTCAACAAC
    CCATGTGACG GGAACAAGTG TATCCATGGC TCGTGTATGG CCATAAACTC ATACTCGTAC
    AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC
    CCCTGTCAGT ATATAGCATG CAAATACGGC CGATGTCGTG TGTCTGGACT GGGCAAAGCC
    TATTGTGAGT GCAACAGCGG CTACACGGGG CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG
    GGGGAACGGG TCCGAGATCA CTATCAGACG CAGCAGGGCT ACGCCGCGTG CCAGAGCACA
    GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC AGCTGTGGGG ACGGGACCTC CTGCTGCGCC
    CCGCTCCGCA GCAAACGCAG GAAATACACC TTCCAATGCA CAGACGGGTC CTCTTTTGTA
    CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009289367.1
    CDS1189..5379
    Translation

    Target ORF information:

    RefSeq Version XM_009291092.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio slit homolog 2 (Drosophila) (slit2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291092.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTTTGTGT TGAAATCAGT GGTGCTCTGT GCGTTGCTCT GCGGGGCTGG AGCCCAGTCG 
    TGCCCGTCCC AGTGCTCCTG CAGCGGGACA GCGGTGGACT GCCACGGCCA GAGTCTGCGG
    AGCGTGCCGC GGAACATTCC CCGCAACGTT GAGCGACTGG ACCTGAATGC AAACAATCTG
    ACTAAAATCA CTAAGGCAGA TTTTGCGGGA CTGAAGAACC TTCGAGTTCT GCAGTTAATG
    GAGAACAAGA TCAGTTCAAT TGAAAGAGGG GCATTTCAGG ATCTACAAGA GCTGGAAAGA
    CTGCGTCTCA ACAGGAATAA TCTTCAGGTT CTTCCGGAGT TGCTGTTTCT GGGCACGACC
    AAACTCTTCA GACTGGACCT GAGTGAAAAT CAAATCCAGG GGATTCCCAG AAAAGCCTTC
    AGAGGATCTA CAGAGATCAA AAACCTGCAG CTGGACTATA ATCAGATTTC CTGCATTGAA
    GATGGAGCGT TCAGAGCTCT GCGGGACTTA GAAGTACTCA CACTGAACAA CAACAATATC
    TCTCGTTTGT CAGTGGCCAG TTTTAACCAC ATGCCCAAGC TCAGGACATT TCGCCTGCAC
    TCTAATAATC TGCTATGTGA TTGCAATGTG GCCTGGCTCT CTGATTGGCT GAGACAGAGG
    CCCCGCCTTG GCCTCTACAC ACAGTGTATG GCTCCACCCT CCCTTAGGGG CCACAACATC
    GCAGAGGTTC AGAAGAAAGA GTTTATGTGT ACAGGGCCCC AGTCACACTC ATCCTGTAGT
    GTGCTGCAGT GTCCTGAGCT CTGCACCTGC AGTAATAATG TAGTGGACTG CCGGGGGAAA
    GGACTTACTG AAATACCCAC CAACCTGCCA GAAACTATCA CTGAGATTCG ACTGGAGCAA
    AACTCCATCA AGATTATTCC AGCAGGAGCT TTTGCACCTT ACAAGCGACT CCGAAGAATA
    GATCTAAGTA ATAATCAAAT AACTGAATTG GCTTCAGACT CCTTTCAAGG TCTCCGTTCT
    TTAAACTCTC TGGTTTTATA TGGGAATAAA ATCACTGAAC TTCCCAAAGG CCTTTTTGAT
    GGACTGTTCT CATTGCAGCT ACTATTGCTG AATGCGAACA AGATCAATTG TTTGCGTGTA
    GATTCTTTCC AAGACCTGCA GAATCTGAAT CTTCTGTCGC TTTATGACAA CAAACTTCAG
    ACCATCGCAA AGGGAACTTT CTCTTCTCTC AGAGCTATCC AAACCCTTCA TCTGGCCCAG
    AACCCCTTCA TGTGTGACTG CCATTTGAAG TGGTTAGCAG ATTACCTGCA AGACAACCCT
    ATTGAGACCA GTGGAGCTCG GTGCACCAGT CCCCGGCGCC TTGCTAACAA ACGTATTGGC
    CAGATCAAGA GCAAAAAGTT CCGCTGTTCA GGCGTAGAAG ACTACCGCTC AAAACTAGGT
    GGGGATTGCT TTGCTGATTT GGCCTGTCCA GAGAAATGTC GATGTGAAGG AACCACAGTG
    GACTGCTCTG GACAAAAGCT CACCAAGATC CCAGACCACA TCCCTCAGTA TACAGCAGAA
    CTCCGCTTGA ATAATAATGA ATTCACAGTG CTGGAGGCTA CAGGAATCTT CAAGAAGCTT
    CCTCAGTTGC GCAAAATCAA TCTTAGCAAC AATAAGATCA CAGATATCGA AGAAGGCACA
    TTTGAAGGGG CCAGTGGAGT AAATGAACTC ATACTGACCA GCAATCGCTT GGAGGGGGTT
    CACTACAGCA TGCTGAAAGG CCTCGGTGGT CTCCGCACAC TGATGTTGAG GAGTAACAGG
    ATAAGCTGTG TCAATAACGG CAGTTTTACC GGTCTGAGCT CTGTGAGGTT GCTTTCCCTC
    TATGATAACC TGATCACCTC CATGTCCCCA GGAGCCTTTG ACACACTACA TTCGCTCTCC
    ACATTGAACT TGTTGGCCAA TCCGTTTAAC TGTAACTGCC ATCTGGCCTG GCTTGGTGAA
    TGGCTCCGCA AGAAACGGAT TGTCACAGGC AACCCCCGCT GTCAGAGCCC CTACTTTCTC
    AAGGAGATCC CTATTCAAGA TGTAGCTGTA CAAGACTTTG CCTGTGAAGA AGGTAACGAT
    GAGAATAGCT GTTCACCACT GGCTCGCTGT CCTGCCGAGT GTTCGTGTTT GGATACAGTG
    GTTCGTTGCA GTAATAAAGG ACTCAAGGTT TTGCCTAAGG GCATTCCTAG AGACGTTACA
    GAGCTCTATC TCGATGGCAA CGAGTTCACA CAAGTCCCTC TGGAACTATC CAATTATAAA
    CACCTCACTC TCATTGATTT GAGTAATAAC CAGATCAGCA CATTGTCCAA CCACAGCTTC
    AGTAACATGT CCGAGCTGCT CACACTAATT TTAAGCTACA ACAGATTAAG GTGTATTCCT
    GCAAAAGCGT TCGACGGCCT CAAGTCTCTG CGCTTGTTGT CTCTCCATGG TAATGATATT
    GCTGTGATCC CAGATGGAGC TTTCAAAGAT CTGTCCTCAC TCTCTCACCT GGCTCTGGGC
    GCAAACCCCC TGTACTGTGA CTGTCACATG CAGTGGCTCT CTGACTGGGT AAAGAGTGGA
    TACAAGGAGC CTGGCATCGC CCGCTGTACC GGTCCTGGAG ACATGACTGA CAAACTGCTG
    CTCACTACAC CCTCTAAGAA ATTCACATGC ACAGGTCCAG TGGATGTAAG CATTTTGGCT
    AAATGTAACC CTTGCTTGTC CAATCCCTGC AAGAATGATG GGACCTGCAG TAACCATCCT
    GTAGATTTTT ACAGATGCAC CTGCCCGTAT GGTTTTAAGG GTCAAGACTG TGAGGAACCA
    ATTCATGCAT GCATCAGTAA CCCCTGTCAG AATGGAGGAA CCTGTCATCT GAAGGATGGA
    GAGGAAAACA CTCACTGGTG TGTGTGTCCA GAAGGCTACA GTGGGTTGTT CTGCGAGTTT
    TCTCCTCCGA TGGTCCTGCC AAGAACAAGT CCATGCGATC ACTACGACTG TGCCAACGGT
    GCACAGTGTG TTGTGAAGGA CACGGACCCC GTGTGTCAGT GTCTGCCAGG TTATGAGGGT
    GTGCACTGTG AAAGACTGGT CAGCGTCAAC TTCATCAACA GAGAATCCTT CCTGCAAATC
    CCCTCCAACC TCATTACAGA ACAGGCCAAT ATATCTCTGC AGATCGCCAC AGATGAGGAT
    AATGGAGTGT TGCTGTACAA AGGAGATAAT GAACACATTG CTGTGGAGCT TTACAGAGGC
    CGACTGAGAG TCAGTTATGA CTCTGGATCA TATCCTCCAT CTGCAATCTA CAGTGTCGAG
    ACAATAAATG ATGGCAGCTT TCATGTAGTG GAGCTGGTGG CAAAGGATCA GTCTCTGTCA
    CTGTCCATTG ATGGAGGAAG CCCGAAATCC ATCAACACTG CCAATAGCCC CAGCCCAGTG
    CCCAGCCCGG CCCCGCTTTA TCTTGGTGGT CTTCCTCAGC AGTCTGGATT GGCCTCGTTG
    CGTCAGGGCT CTGGCAGGAA TGGCTCTAGT TTCCATGGCT GCATCCGAAA TCTCTACATT
    AATGACCAAC TCCAGGATCT CACCCAGTTT TTGCTGCAGG AGGGTGTTGT GCCCGGATGC
    CAGCCATGCC AGCGGAGCAT GTGTGCTCAT GGCCAGTGCC ATGCCACTGG ACAGTCCTCT
    TTCAGCTGTG AGTGTGAGGC GGGATGGACT GGCCCGCTCT GTGACCAGCA GGTCAACAAC
    CCATGTGACG GGAACAAGTG TATCCATGGC TCGTGTATGG CCATAAACTC ATACTCGTAC
    AGCTGCCGTT GTCTTCCAGG CTTTGCAGGT GTGCTGTGTG ATGAAGAGGA GCAGCTCAGC
    CCCTGTCAGT ATATAGCATG CAAATACGGC CGATGTCGTG TGTCTGGACT GGGCAAAGCC
    TATTGTGAGT GCAACAGCGG CTACACGGGG CAAAGCTGCG ACAGAGAGAT GGCGTGTCGG
    GGGGAACGGG TCCGAGATCA CTATCAGACG CAGCAGGGCT ACGCCGCGTG CCAGAGCACA
    GAGAAGGTCT CGCGTTTAGA GTGTCGGGGC AGCTGTGGGG ACGGGACCTC CTGCTGCGCC
    CCGCTCCGCA GCAAACGCAG GAAATACACC TTCCAATGCA CAGACGGGTC CTCTTTTGTA
    CAGGAGGTGG AAAAGGTTGT CAAGTGTGGC TGCACAAAAT GTCCATCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.