SPTB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTB
Entrez Gene ID	58040
Full Name	spectrin, beta, erythrocytic
Synonyms	wu:fa09g12
General protein information	Preferred Names spectrin, beta, erythrocytic Names spectrin beta chain, erythrocyte b-spectrin beta-spectrin fa09g12 riesling ris
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005158849.3	XP_005158906.1	spectrin beta chain, erythrocyte isoform X1
XM_005158849.4	XP_005158906.1	spectrin beta chain, erythrocyte isoform X1
XM_005158850.3	XP_005158907.1	spectrin beta chain, erythrocyte isoform X2
XM_005158851.4	XP_005158908.1	spectrin beta chain, erythrocyte isoform X2
NM_131525.1	NP_571600.1	spectrin beta chain, erythrocyte
XM_005158850.2	XP_005158907.1	spectrin beta chain, erythrocyte isoform X2
XM_005158851.3	XP_005158908.1	spectrin beta chain, erythrocyte isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPTBN1	NP_001186354.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010895	XP_309796.2
Caenorhabditis elegans (roundworm)	unc-70	NP_001024053.1
Canis lupus familiaris (dog)	SPTBN1	XP_531827.2
Pan troglodytes (chimpanzee)	SPTBN1	XP_001154155.1
	SPTBN1	XP_001114804.1
Bos taurus (cattle)	SPTBN1	NP_001179205.1
Drosophila melanogaster (fruit fly)	beta-Spec	NP_001259660.1
Homo sapiens (human)	SPTBN1	NP_003119.2
Mus musculus (house mouse)	Sptbn1	NP_787030.2
Rattus norvegicus (Norway rat)	Sptbn1	NP_001013148.1
Danio rerio (zebrafish)	sptb	NP_571600.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
Tang Q, Iyer S, Lobbardi R, Moore JC, Chen H, Lareau C, Hebert C, Shaw ML, Neftel C, Suva ML, Ceol CJ, Bernards A, Aryee M, Pinello L, Drummond IA, Langenau DM
The Journal of experimental medicine214(10)2875-2887(2017 Oct)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Smith LC, Clark JC, Bisesi JH Jr, Ferguson PL, Sabo-Attwood T
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
Moore FE, Garcia EG, Lobbardi R, Jain E, Tang Q, Moore JC, Cortes M, Molodtsov A, Kasheta M, Luo CC, Garcia AJ, Mylvaganam R, Yoder JA, Blackburn JS, Sadreyev RI, Ceol CJ, North TE, Langenau DM
The Journal of experimental medicine213(6)979-92(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPTB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa32569	NM_131525.1 Latest version!	Danio rerio spectrin, beta, erythrocytic (sptb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa22741	XM_005158849.4 Latest version!	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa22742	XM_005158850.3 Latest version!	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa22742	XM_005158851.4 Latest version!	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa22741	XM_005158849.3	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa22742	XM_005158850.2	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa22742	XM_005158851.3	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTB ( NM_131525.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571600.1 Danio rerio spectrin, beta, erythrocytic (sptb), mRNA.

Price & Availability↑

CloneID	ODa32569
Clone ID Related Accession (Same CDS sequence)	NM_131525.1
Accession Version	NM_131525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, erythrocyte
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF262336.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF262336.1, BC162568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505385, SAMEA3505389 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAATGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GACGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCGCG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGCAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CTCGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCTAG CCGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAGCTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGACGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGAGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCA 
GCCCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGAAT 
TTCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTGATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAC AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCTTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG TGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGAGG TGTGGGCACA GCTACAGGAA GAGATGGCCA AACGAAGAGA ACGGCTCTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAGTTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGTGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCTGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGAYGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAAGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCTCTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCAA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTCA AACCTGGACA CCTCTCCATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571600.1
CDS	1..7074
Translation	MATMTSTTDYDNAEITQQYSRINTRFELSDDELDNDNSSARLFERSRIKALADEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQQLCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAASRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLNFLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVQQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQEVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKnullVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	NM_131525.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131525.1

ORF Insert Sequence:

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAATGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GACGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCGCG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGCAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CTCGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCTAG CCGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAGCTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGACGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGAGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCA 
GCCCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGAAT 
TTCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTGATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAC AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCTTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG TGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGAGG TGTGGGCACA GCTACAGGAA GAGATGGCCA AACGAAGAGA ACGGCTCTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAGTTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGTGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCTGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGAYGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAAGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCTCTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCAA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTCA AACCTGGACA CCTCTCCATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005158849.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158906.1 Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.

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CloneID	ODa22741
Clone ID Related Accession (Same CDS sequence)	XM_005158849.3 , XM_005158849.4
Accession Version	XM_005158849.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	spectrin beta chain, erythrocyte isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC CCTGTTTGTG TCGTGCGGTG CCCGTGAGCC AGACCCGGCT GAGGGAGGTC 
AAACTTAGCT TGAAGACCAG CAGAGAGACT ATTGCAATCC GCTCTACCCC AGTCGTCCGA 
GAAGTCAGCA GCCGGCAGTA TACTACAGTT AAAGATGTCC CAACCCGGAC CAATGCACCA 
GGCAGTCGGA GCAGGAGCAC CCCACCAGTT TACAGGGCCC AGAGCAAGAG AGGGGCCAAA 
AACAACAGTC CTCATATTGG TGAACCTGAG CACCTGCCGG AGAGAAATGG AACTTTATTG 
GGGATCACAC CTAAACTGGC ACCCATGACT CTCACTTCAA TCTCCTCAAC CTCATCATCA 
TCATCTTCCT GCACCCCAAG GGGCCATCGG CCTTCACCTC TGCGATCCTC ACCATCTTGT 
CCCCTATCCC CGTCCCGGGG TATGAGACGA GCACGCTGGT TGAGCTACAG TGCTTCCAAC 
ATTTGCTTCA ATAATTCCAT TCTAGACGGC CGCTTCAGAC AACTGCAAGA TGAGCGAGAG 
GCTGTCCAGA AGAAGACTTT TACAAAATGG GTAAACTCCA TACTAGCCAG AGTCAGTTGC 
CGAATCTCAG ATCTCTATCT AGACCTGCGG GATGGGAGAA TGCTCATCAA ACTATTGGAA 
GTTCTGTCAG GGGAGAGATT GCCCAAGCCG ACCAAGGGCC GAATGCGTAT CCATTGTCTG 
GAGAATGTAG ACAAAGCTCT ACAGTTTCTA AAGGAACAGA AGGTGCACTT AGAGAACATG 
GGATCACATG ATATTGTAGA TGGGAACCAC CGGCTCATCC TGGGTCTCAT CTGGACCATC 
ATTCTTCGCT TCCAGATTCA AGATATCATA GTAGAAACAG GACAAGCTGA TCAGACGGGC 
CGGCAAGAGA CTCGCTCAGC CAAAGATGCA CTGCTCCTCT GGTGCCAGAT GAAGACTGCA 
GGGTACCCAA ACATAAATAT CACCAATTTC ACCACGAGCT GGAAAGACGG AATGGCTTTT 
AATGCTCTCA TTCACAAGCA CAGGCCAGAT CTGGTGGACT ATGGCAATCT AAAGAGGTCC 
AATCCCACAC ACAACCTGCA ACAGGCTTTT AATGTAGCTG AAAAGAAGCT TGGTGTCACC 
AAGTTATTGG ACCCAGAAGA TGTGTTCACT GAGAATCCAG ATGAGAAGTC CATAATCACA 
TATGTTGTTG CATTTTACCA CTATTTCTCC AAGATGAAAG CTCTGGCAGT TGAGGGCAAG 
CGAATTGGAA AGGTGCTGGA CCAGGCCATT GAAACTGAGA AGATGATTGA GAAGTATGAG 
ACACTGTCAT CGGACCTGCT AACATGGATT GAGCAAACCA TCGTAGTGCT AAACAACCGC 
AAGCTCGCTA ACTCCCTTAC AGGCGTCCAA CAGCAACTGC AGGCTTTCAA CTCTTACCGT 
ACTGTGGAGA AACCACCAAA GTTTCAGGAG AAAGGAAACT TGGAAGTACT GCTGTTTACC 
ATCCAGAGCA GAATGAGGGC CAACAATCAG AAAGTCTACA CACCTAAAGA GGGTGCATTG 
GTGTCAGATA TCAACAAGGC TTGGGAGAGG TTGGAGAAGG CAGAATATGA TCGTGAGCGG 
GTCTTAAGAG ATGAACTCAT TAGGCAAGAG AAGCTGGAAC AGATGGCTCG TCGCTTTGAC 
AGAAAGGCAG CCATGAGAGA AACCTGGCTT ATGGAGAATC AAAGACTGGT TTCACAGGAC 
AACTTTGGCT ATGACTTACC AGCAGTGGAG GCAGCTAAGA AGAAACATGA TGCTATAGAG 
ACAGACATTG CCGCATATGA AGAACGTGTT CAGGCACTGG TTGCCCTGTC TAAAGAACTG 
GAGTCAGAAA GATACCATGA TGCTAAACGC ATTGATGCTA GAAAGGACAA TATCTTGAGG 
CTGTGGGATT ACCTACAGGA GCTCCTAAAA GCTCGCAGAG GTCGCCTAGA CAAGAACCTC 
ACCCTTCAGA GAATTTTCCA GGAAATGCTC CACATCATCA GCTGGATGGA TGAGATGAAG 
GGTCGTCTGT TGTCCCCAGA CTTTGGAAAA CACTTATTGG AAGTGGAAGA CTTGTTGCAG 
AAACATTCAT TGGTAGAGGC TGATATAGCA GTGCAGGCAG AAAGAGTGAA ATCAGCCAAT 
GCAGCTGCTC TTAAATTTGC TAATGGTGAC AGTTATAAAC CATGTGACCC CCAAGTTATC 
CGTGATCGGG TACAGCATTT GGACCTGTGC TACCAGGAGT TGTGTGCACT GGCAGCTCAA 
AGAAAAGCAC GCCTTGAGCA GTCACGGCGC CTTTGGAACT TTTTATGGGA GATAGCAGAG 
CTGGAAAGCT GGATTCGTGA AAGGGAGCAA ATATTTTCCT CTCTTGACTA TGGTAAGGAC 
CTGACCAGTG TGTTGATACT GCAAAGCAAA CACAGTGTCT TTGAGGATGA GCTTGCAGCA 
AGACGGGACA ACTTGAAGCA GGTGATGGAC GAGGGAGAGA GCATGATCCA GATCAAGCAC 
TTGGGTTCCC CCAAAGTGCA GCAACGCATG AATGATGTAC AAAGACAGTG GCAACAACTG 
GAGGAACTGG CTGCATTCCG TAAACAGAAT CTGCAAGACA CCCAACGTTT CTTCCAGTTT 
CAGGGTGATG CTGATGACCT CAAGGCTTGG CTGGTCGATG CAATGAGACA GATGAGCAGT 
GACGATGTTG GGCATGATGA GTACACTACA CAGCGACTGC TCAAGAAACA CCGTGACTTA 
AGAGATGAGG CTGCTAAGAA TGGAGCCACC ATTGATGCTC TATCCAAACA GGCCAATGCA 
CTTCCTGAAG AACTACGGAA TACCCCTGAC ATCCAGGGCC GTCTGAATGA TATACGGGAT 
ATGTATATTG AGCTTCTGAC CCTCTCTGAT TTGAGGCAGA AGAAACTGGA TGACACCATG 
GCTCTTTACA CTATCTTCAG TGAGACAGAT GCTTGTGAGC TCTGGATGGG CCAGAAAGAG 
ACCTGGCTGG TTGGGTTAGA GACACCTGAG AATTTGGAAG ATCTAGAAAT TGTACAAAAC 
AGGCTAAGCA TTCTTGCTCA AGAAATGGGT AATATGCAGA CTCGAGTTGA TAACGTTAAT 
AAAGCAGCCA AGCAACTTGA AGACAGCAGA CATCCACAAA CAAAACAAGT GAAAGACTGT 
CAAATTCGTC TCAATAAGAG ATGGGAGGCT TTTAAAGCAA TGGTCGAAGA TAAGAAACAT 
AGGGTAGATT CTGCTCTTAG CCTACATAAC TATGATCTTG ATTGTGATGA GACAGAATCA 
TGGATAAAAG AAAAGACGAG AGTCATTGAG TCTACACAGG ATCTTGGGAA TGACCTTGCT 
GCTGTCATTA CTATTCAAAG GAAGCTTTTT GGCATGGAAA GAGACCTTGC TGCCATCCAA 
GATAAGTTGG ATTCCCTGCG TGACGAGGCC CAGAAGCTTG TAAAGGAGCA CCCAGAAAAT 
GCTTCTGATA TACTTGCCAG ACAGGAAGAG TTGGATGCAG CATGGGACAC CTTGAAACGT 
ACCCTGAAAG ACCGTGAAGA CTCTCTTGGG GAGGTCAGCA AGCTGCAGAC CTTCTTACAG 
GACATGGACG ATTTTCAAGC TTGGTTGTTC AAGTCACAGA AGGCTGTTGC TTCTGAAGAT 
ATGCCAGATG GTTTACCAGA GGCTGAGCAA CTCCTGAATC TCCATGATGC TTTGAGGCAT 
GATATGGATG GCCATGAGGA AGACTACCAT CGTGTGAAAG ACACAGGAGC TGCTGTTATT 
CAAGGTCAAG AAGATGATCC TCAGTACCAG CAACTGGAAC AGAGGCTGGA GGGTCTTGAT 
AAGGGCTGGG GTGAGCTTCA CAAAATGTGG GACAGTCGCA AGAACTTCCT AGATCAGGGT 
CTCGGTTTCC AACAATTCAT GAGAGATGCC AAACAGGCTG ATGCCATCTT AAATAATCAG 
GAATACACTC TAGCCCACGT AGACAAGCCT GACACCCTTG ATGGAGCAGA GAAGGCCTTG 
AAGAAACATG AGGACTTTGT TACCACCATG GATGCTAATA AGGAGAAGAT ACTTAGCACA 
CTGGAGACTG GTCAGAGGCT GGTGGATAGT GAAAACCTTT ACTCTGGAAA AGTGAAAGAC 
AAGATGAGTT CAATCGAGGA AAGGAACAAA AAGAACCAAG ACAAAGCCAA GGAAGTCTCA 
GGGAAACTTA AAGACAACAG GGAACTTCAG CACTTCCTTC AAAACACTCA GGATCTTACA 
TTGTGGATTA ATGAGAAAAT GCTGACTGCA CAAGATACAT CATATGATGA GGCTCGTAAC 
CTTCATAGCA AGTGGCAAAA ACACCAAGCT TTCATGGCAG AACTAGCATC AAACAAGGAT 
TGGCTTCATA ACATTGATAA GGAGGGACAG GAACTGATGG AATCCAAGCC TGAGTTTGAG 
CCTATTGTTA AGGATCGCCT TGCCAAGCTT CATGAACTAT GGGATAAGCT GGAGAGCACC 
ACACAAGAAA AGGCTCGGCT ACTGTTTGAT GCAAACCGTT CTGAGCTGTT CGATCAGAGC 
CTAGCTGACC TAAAGAAATG GTTAGCTGAA TTGCAGCAGC AACTTCAGGG TGATGTGGAA 
GAAGAAGTTA AGGATCTTAC CAGTGCCAAC ATCCTGCTAA AGAAACATCA GATAACAGAG 
AATCAGGTTC GGGACCGTGC ACGAGAGCTT GAAGAACTTC AGGAGGCTGT AGAGCAGCAT 
GGTTCTCTTA GAGAGGACCA GCCTGAGCTT GAGATTGAGC AACAAAATCT TCAGAGAGAT 
TTCCAGAAGC TCCTCACACC TTTATCACAA CGCAAGGGAA AGCTGGAGGC TGCTAAGGCA 
GTGCACCAGT TCTTTAGAGA TCTTGCTGAT GAAATACTCT GGGTTAATGA AAGGTTACCA 
ATGGCTATGT CAGATGATCA TGGTAACAAT CTTCAGACTG TCCAACTTTT GCTTAAGAAG 
AACCAATCCT TACAAAAAGA GATTGATGGT CATCAACCAC GCATTGATGA AGTGTTAGAG 
CGGGGTCGGC GAATGGCAGC AGCAGCAGAG GGCAGTCCAG AGGAAGAACG CATGTCTGAG 
GAGATGAAGA AGCTTCAGGT GGTGTGGGCA CAGCTACAGG AAGAGATGGC AAAACGAAGA 
GAACGGCTTT ATGGCTCTAA TGAGGCCCAA CAGTATTACA ATGATGCAGA TGATTCTGAG 
GCCTGGATAG GTGAACAAGA ACTCTACATG ATTGCTGATG AGATGGCAAA GGATGAACAG 
AGTGCTATGA TCATGCTGAA GCGGCACCTG GTTCTTAAGC AGACAGTGGA TGACTATGCA 
TATTCCATCC AGCAATTGGC AGACCGTGCC CAGAAGATGC TGGCTGAAGA GCACCCAGAT 
GGTGAGGCTA TCATCCGAAG ACAAGGACAA GTTGATAAGC AGTATGCTGG GCTGAAGGAG 
CTGGCAGAAG ACCGCAAAAA GAAGCTGGAC CACACATATC ACCATTTCCT GCTGAGTCGG 
GAAGTGGAGG ATCTAGAGCA GTGGATTGCA GAGCGAGATG TAGTGGCTTC ATCACAAGAA 
ATGGGCCAAG ACCTTGATCA TGTGACGATC TTGCGAGACA AGTTCAGGGA GTTTGCACGG 
GAAACAGGGA CAGTGGGTCA AGAACGTGTA GATACTGTGA ACCGGATCAT AGATGAACTA 
ATTGAAGGAG GCCACAGTGA GTCAGCGACA TTGGCCGAGT GGAAGGATGG AGTAAATGAG 
AGTTGGGCAG ATTTGCTGGA GCTCATTGAC ACTCGTGCCC AGCTCCTCAC ATCCTCTTAT 
GACCTCCTTA AGTATTTTTA TGATGGAAAA GAGCTGGTTG GTCACATTGA GGAAAAGAAG 
AATGAACTTC CTGAAGACTT GGGAGAAGAC TTCAGCAAAG CAGAATCATT CCACCGAATG 
CATGCTGCAT TTGAGAGGGA TATCAGCTCC TTGGGCAAAC AGGTAAAACA GTTCCAGGAG 
ACAGCAGCTA GATTGCATGC CCAGTATGCA GGAGATCAGG CTACTGCCAT TCAAGCAACT 
GAAAAAGAGG TTGTTGAGGC CTGGAAGGGT CTGTTGGATG CTTGTGCGGG TAGGAGGAAA 
CAGCTGGAGG AGACAGCAGA CAAATTCCGC TTTTTCACCA TGGTGAGAGA CCTAATGGCC 
TGGATGGAAA GCATCCTTCA ACAGATAGAG ACACAGGAAA AACCAAGAGA CGTCTCCTCA 
GTGGAGCTTC TGATGAAGTA TCATCAAGGG ATCCGTGCTG AAATTGAGAC CCGTGGGCCA 
AAGTTTAATC AGTGTGTGCA ACTTGGACAG GCTCTCCTGG AACGCAAGCA CAAGGACTCT 
GCAGAGATTA AAGAGAAGCT GATGCAACTG GTAGAGAAGA GAAAAGAGAT GATGCTCAAA 
TGGGATGATC GATGGGACTG GCTCAGACTT TTACTGGAGG TATGTCAGTT TGCTCGAGAT 
GCTTCTGTGG CAGAGGCATG GTTGATTGCC CAGGAGCCAT ATGTGGCCAG CAAGGACGTG 
GGTCAGACAG TCGATGAGGT TGAAAAACTG CTCAAAAGGC ATGAAGCATT TGAAAAATCT 
ACTGCAACAT GGGAAGAGCG TTTCTCAGCA TTGGAACGTC TTACAACATT GGAGTTGTTG 
GAAATTCGGA AACAACAACA AGAAATACAA CAGTACAGAC AAGAGGCAGA GAAAGAAAGC 
AGGAGAGAGG ACACTGGTTT TGCAGAGGAG TCTTCACAAC TTTACCCTAC AGAAGAACAG 
TCTCTGTCTG GTTTAGGAGT AATAGAGCCT AGTTCTGGTG GAGTTGATGG CACAGCAGGG 
GAATCCACTG TGCCTTTAAT CTCTGAGATG CAGGAAAGTG TCTCACTAGA GCTGGACCCT 
TCCACATCCA TTCAAGTTAC CAAAGAGACA GAAAAAGCTG CCTCGCTTCC TACAGAATCT 
ACACAAGTTC AACCAGTCCT GATGGAGGGA ACTCTAGCCC GCAAGCATGA GTTGGAGGGA 
CCTAACAAGA AGGCACCCAA CAGGTCCTGG AACAATCTGT ATTGTGTGCT TAAACCTGGA 
CACCTCTCTA TCTACAAGGA TGCCAAGAGC TTTAGTCACA GTGTAACCTT CCATGGTGAG 
GAACCCCTGA CCCTCACCAA TTCTTCATGC GAGATCTTGA CCAACTATAA GAAAAAGAAG 
CAGGTGTTCA AACTCCGACT TGGAGATGGA AGTGAATATC TGTTCCAGTG TAAAGACGAG 
GAGGAGCTTC AGAACTGGAC TCAGGCTATT GAGCAGGCTG CACAAGTTCA AATAGAAGAA 
CCAGTTGCAG GGCCATCAGG AGTGAAGGCA CTAAGCCTAC CTCCACCTTC CTCCACGACT 
CTAGAGGTTC CCTCAACGAA GAAGGACAAG GAAAAGAGGT TTAGTCTGTT TGCTAAGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158906.1
CDS	87..7532
Translation	MSSPCLCRAVPVSQTRLREVKLSLKTSRETIAIRSTPVVREVSSRQYTTVKDVPTRTNAPGSRSRSTPPVYRAQSKRGAKNNSPHIGEPEHLPERNGTLLGITPKLAPMTLTSISSTSSSSSSCTPRGHRPSPLRSSPSCPLSPSRGMRRARWLSYSASNICFNNSILDGRFRQLQDEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQVVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	XM_005158849.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158849.3

ORF Insert Sequence:

ATGAGCAGCC CCTGTTTGTG TCGTGCGGTG CCCGTGAGCC AGACCCGGCT GAGGGAGGTC 
AAACTTAGCT TGAAGACCAG CAGAGAGACT ATTGCAATCC GCTCTACCCC AGTCGTCCGA 
GAAGTCAGCA GCCGGCAGTA TACTACAGTT AAAGATGTCC CAACCCGGAC CAATGCACCA 
GGCAGTCGGA GCAGGAGCAC CCCACCAGTT TACAGGGCCC AGAGCAAGAG AGGGGCCAAA 
AACAACAGTC CTCATATTGG TGAACCTGAG CACCTGCCGG AGAGAAATGG AACTTTATTG 
GGGATCACAC CTAAACTGGC ACCCATGACT CTCACTTCAA TCTCCTCAAC CTCATCATCA 
TCATCTTCCT GCACCCCAAG GGGCCATCGG CCTTCACCTC TGCGATCCTC ACCATCTTGT 
CCCCTATCCC CGTCCCGGGG TATGAGACGA GCACGCTGGT TGAGCTACAG TGCTTCCAAC 
ATTTGCTTCA ATAATTCCAT TCTAGACGGC CGCTTCAGAC AACTGCAAGA TGAGCGAGAG 
GCTGTCCAGA AGAAGACTTT TACAAAATGG GTAAACTCCA TACTAGCCAG AGTCAGTTGC 
CGAATCTCAG ATCTCTATCT AGACCTGCGG GATGGGAGAA TGCTCATCAA ACTATTGGAA 
GTTCTGTCAG GGGAGAGATT GCCCAAGCCG ACCAAGGGCC GAATGCGTAT CCATTGTCTG 
GAGAATGTAG ACAAAGCTCT ACAGTTTCTA AAGGAACAGA AGGTGCACTT AGAGAACATG 
GGATCACATG ATATTGTAGA TGGGAACCAC CGGCTCATCC TGGGTCTCAT CTGGACCATC 
ATTCTTCGCT TCCAGATTCA AGATATCATA GTAGAAACAG GACAAGCTGA TCAGACGGGC 
CGGCAAGAGA CTCGCTCAGC CAAAGATGCA CTGCTCCTCT GGTGCCAGAT GAAGACTGCA 
GGGTACCCAA ACATAAATAT CACCAATTTC ACCACGAGCT GGAAAGACGG AATGGCTTTT 
AATGCTCTCA TTCACAAGCA CAGGCCAGAT CTGGTGGACT ATGGCAATCT AAAGAGGTCC 
AATCCCACAC ACAACCTGCA ACAGGCTTTT AATGTAGCTG AAAAGAAGCT TGGTGTCACC 
AAGTTATTGG ACCCAGAAGA TGTGTTCACT GAGAATCCAG ATGAGAAGTC CATAATCACA 
TATGTTGTTG CATTTTACCA CTATTTCTCC AAGATGAAAG CTCTGGCAGT TGAGGGCAAG 
CGAATTGGAA AGGTGCTGGA CCAGGCCATT GAAACTGAGA AGATGATTGA GAAGTATGAG 
ACACTGTCAT CGGACCTGCT AACATGGATT GAGCAAACCA TCGTAGTGCT AAACAACCGC 
AAGCTCGCTA ACTCCCTTAC AGGCGTCCAA CAGCAACTGC AGGCTTTCAA CTCTTACCGT 
ACTGTGGAGA AACCACCAAA GTTTCAGGAG AAAGGAAACT TGGAAGTACT GCTGTTTACC 
ATCCAGAGCA GAATGAGGGC CAACAATCAG AAAGTCTACA CACCTAAAGA GGGTGCATTG 
GTGTCAGATA TCAACAAGGC TTGGGAGAGG TTGGAGAAGG CAGAATATGA TCGTGAGCGG 
GTCTTAAGAG ATGAACTCAT TAGGCAAGAG AAGCTGGAAC AGATGGCTCG TCGCTTTGAC 
AGAAAGGCAG CCATGAGAGA AACCTGGCTT ATGGAGAATC AAAGACTGGT TTCACAGGAC 
AACTTTGGCT ATGACTTACC AGCAGTGGAG GCAGCTAAGA AGAAACATGA TGCTATAGAG 
ACAGACATTG CCGCATATGA AGAACGTGTT CAGGCACTGG TTGCCCTGTC TAAAGAACTG 
GAGTCAGAAA GATACCATGA TGCTAAACGC ATTGATGCTA GAAAGGACAA TATCTTGAGG 
CTGTGGGATT ACCTACAGGA GCTCCTAAAA GCTCGCAGAG GTCGCCTAGA CAAGAACCTC 
ACCCTTCAGA GAATTTTCCA GGAAATGCTC CACATCATCA GCTGGATGGA TGAGATGAAG 
GGTCGTCTGT TGTCCCCAGA CTTTGGAAAA CACTTATTGG AAGTGGAAGA CTTGTTGCAG 
AAACATTCAT TGGTAGAGGC TGATATAGCA GTGCAGGCAG AAAGAGTGAA ATCAGCCAAT 
GCAGCTGCTC TTAAATTTGC TAATGGTGAC AGTTATAAAC CATGTGACCC CCAAGTTATC 
CGTGATCGGG TACAGCATTT GGACCTGTGC TACCAGGAGT TGTGTGCACT GGCAGCTCAA 
AGAAAAGCAC GCCTTGAGCA GTCACGGCGC CTTTGGAACT TTTTATGGGA GATAGCAGAG 
CTGGAAAGCT GGATTCGTGA AAGGGAGCAA ATATTTTCCT CTCTTGACTA TGGTAAGGAC 
CTGACCAGTG TGTTGATACT GCAAAGCAAA CACAGTGTCT TTGAGGATGA GCTTGCAGCA 
AGACGGGACA ACTTGAAGCA GGTGATGGAC GAGGGAGAGA GCATGATCCA GATCAAGCAC 
TTGGGTTCCC CCAAAGTGCA GCAACGCATG AATGATGTAC AAAGACAGTG GCAACAACTG 
GAGGAACTGG CTGCATTCCG TAAACAGAAT CTGCAAGACA CCCAACGTTT CTTCCAGTTT 
CAGGGTGATG CTGATGACCT CAAGGCTTGG CTGGTCGATG CAATGAGACA GATGAGCAGT 
GACGATGTTG GGCATGATGA GTACACTACA CAGCGACTGC TCAAGAAACA CCGTGACTTA 
AGAGATGAGG CTGCTAAGAA TGGAGCCACC ATTGATGCTC TATCCAAACA GGCCAATGCA 
CTTCCTGAAG AACTACGGAA TACCCCTGAC ATCCAGGGCC GTCTGAATGA TATACGGGAT 
ATGTATATTG AGCTTCTGAC CCTCTCTGAT TTGAGGCAGA AGAAACTGGA TGACACCATG 
GCTCTTTACA CTATCTTCAG TGAGACAGAT GCTTGTGAGC TCTGGATGGG CCAGAAAGAG 
ACCTGGCTGG TTGGGTTAGA GACACCTGAG AATTTGGAAG ATCTAGAAAT TGTACAAAAC 
AGGCTAAGCA TTCTTGCTCA AGAAATGGGT AATATGCAGA CTCGAGTTGA TAACGTTAAT 
AAAGCAGCCA AGCAACTTGA AGACAGCAGA CATCCACAAA CAAAACAAGT GAAAGACTGT 
CAAATTCGTC TCAATAAGAG ATGGGAGGCT TTTAAAGCAA TGGTCGAAGA TAAGAAACAT 
AGGGTAGATT CTGCTCTTAG CCTACATAAC TATGATCTTG ATTGTGATGA GACAGAATCA 
TGGATAAAAG AAAAGACGAG AGTCATTGAG TCTACACAGG ATCTTGGGAA TGACCTTGCT 
GCTGTCATTA CTATTCAAAG GAAGCTTTTT GGCATGGAAA GAGACCTTGC TGCCATCCAA 
GATAAGTTGG ATTCCCTGCG TGACGAGGCC CAGAAGCTTG TAAAGGAGCA CCCAGAAAAT 
GCTTCTGATA TACTTGCCAG ACAGGAAGAG TTGGATGCAG CATGGGACAC CTTGAAACGT 
ACCCTGAAAG ACCGTGAAGA CTCTCTTGGG GAGGTCAGCA AGCTGCAGAC CTTCTTACAG 
GACATGGACG ATTTTCAAGC TTGGTTGTTC AAGTCACAGA AGGCTGTTGC TTCTGAAGAT 
ATGCCAGATG GTTTACCAGA GGCTGAGCAA CTCCTGAATC TCCATGATGC TTTGAGGCAT 
GATATGGATG GCCATGAGGA AGACTACCAT CGTGTGAAAG ACACAGGAGC TGCTGTTATT 
CAAGGTCAAG AAGATGATCC TCAGTACCAG CAACTGGAAC AGAGGCTGGA GGGTCTTGAT 
AAGGGCTGGG GTGAGCTTCA CAAAATGTGG GACAGTCGCA AGAACTTCCT AGATCAGGGT 
CTCGGTTTCC AACAATTCAT GAGAGATGCC AAACAGGCTG ATGCCATCTT AAATAATCAG 
GAATACACTC TAGCCCACGT AGACAAGCCT GACACCCTTG ATGGAGCAGA GAAGGCCTTG 
AAGAAACATG AGGACTTTGT TACCACCATG GATGCTAATA AGGAGAAGAT ACTTAGCACA 
CTGGAGACTG GTCAGAGGCT GGTGGATAGT GAAAACCTTT ACTCTGGAAA AGTGAAAGAC 
AAGATGAGTT CAATCGAGGA AAGGAACAAA AAGAACCAAG ACAAAGCCAA GGAAGTCTCA 
GGGAAACTTA AAGACAACAG GGAACTTCAG CACTTCCTTC AAAACACTCA GGATCTTACA 
TTGTGGATTA ATGAGAAAAT GCTGACTGCA CAAGATACAT CATATGATGA GGCTCGTAAC 
CTTCATAGCA AGTGGCAAAA ACACCAAGCT TTCATGGCAG AACTAGCATC AAACAAGGAT 
TGGCTTCATA ACATTGATAA GGAGGGACAG GAACTGATGG AATCCAAGCC TGAGTTTGAG 
CCTATTGTTA AGGATCGCCT TGCCAAGCTT CATGAACTAT GGGATAAGCT GGAGAGCACC 
ACACAAGAAA AGGCTCGGCT ACTGTTTGAT GCAAACCGTT CTGAGCTGTT CGATCAGAGC 
CTAGCTGACC TAAAGAAATG GTTAGCTGAA TTGCAGCAGC AACTTCAGGG TGATGTGGAA 
GAAGAAGTTA AGGATCTTAC CAGTGCCAAC ATCCTGCTAA AGAAACATCA GATAACAGAG 
AATCAGGTTC GGGACCGTGC ACGAGAGCTT GAAGAACTTC AGGAGGCTGT AGAGCAGCAT 
GGTTCTCTTA GAGAGGACCA GCCTGAGCTT GAGATTGAGC AACAAAATCT TCAGAGAGAT 
TTCCAGAAGC TCCTCACACC TTTATCACAA CGCAAGGGAA AGCTGGAGGC TGCTAAGGCA 
GTGCACCAGT TCTTTAGAGA TCTTGCTGAT GAAATACTCT GGGTTAATGA AAGGTTACCA 
ATGGCTATGT CAGATGATCA TGGTAACAAT CTTCAGACTG TCCAACTTTT GCTTAAGAAG 
AACCAATCCT TACAAAAAGA GATTGATGGT CATCAACCAC GCATTGATGA AGTGTTAGAG 
CGGGGTCGGC GAATGGCAGC AGCAGCAGAG GGCAGTCCAG AGGAAGAACG CATGTCTGAG 
GAGATGAAGA AGCTTCAGGT GGTGTGGGCA CAGCTACAGG AAGAGATGGC AAAACGAAGA 
GAACGGCTTT ATGGCTCTAA TGAGGCCCAA CAGTATTACA ATGATGCAGA TGATTCTGAG 
GCCTGGATAG GTGAACAAGA ACTCTACATG ATTGCTGATG AGATGGCAAA GGATGAACAG 
AGTGCTATGA TCATGCTGAA GCGGCACCTG GTTCTTAAGC AGACAGTGGA TGACTATGCA 
TATTCCATCC AGCAATTGGC AGACCGTGCC CAGAAGATGC TGGCTGAAGA GCACCCAGAT 
GGTGAGGCTA TCATCCGAAG ACAAGGACAA GTTGATAAGC AGTATGCTGG GCTGAAGGAG 
CTGGCAGAAG ACCGCAAAAA GAAGCTGGAC CACACATATC ACCATTTCCT GCTGAGTCGG 
GAAGTGGAGG ATCTAGAGCA GTGGATTGCA GAGCGAGATG TAGTGGCTTC ATCACAAGAA 
ATGGGCCAAG ACCTTGATCA TGTGACGATC TTGCGAGACA AGTTCAGGGA GTTTGCACGG 
GAAACAGGGA CAGTGGGTCA AGAACGTGTA GATACTGTGA ACCGGATCAT AGATGAACTA 
ATTGAAGGAG GCCACAGTGA GTCAGCGACA TTGGCCGAGT GGAAGGATGG AGTAAATGAG 
AGTTGGGCAG ATTTGCTGGA GCTCATTGAC ACTCGTGCCC AGCTCCTCAC ATCCTCTTAT 
GACCTCCTTA AGTATTTTTA TGATGGAAAA GAGCTGGTTG GTCACATTGA GGAAAAGAAG 
AATGAACTTC CTGAAGACTT GGGAGAAGAC TTCAGCAAAG CAGAATCATT CCACCGAATG 
CATGCTGCAT TTGAGAGGGA TATCAGCTCC TTGGGCAAAC AGGTAAAACA GTTCCAGGAG 
ACAGCAGCTA GATTGCATGC CCAGTATGCA GGAGATCAGG CTACTGCCAT TCAAGCAACT 
GAAAAAGAGG TTGTTGAGGC CTGGAAGGGT CTGTTGGATG CTTGTGCGGG TAGGAGGAAA 
CAGCTGGAGG AGACAGCAGA CAAATTCCGC TTTTTCACCA TGGTGAGAGA CCTAATGGCC 
TGGATGGAAA GCATCCTTCA ACAGATAGAG ACACAGGAAA AACCAAGAGA CGTCTCCTCA 
GTGGAGCTTC TGATGAAGTA TCATCAAGGG ATCCGTGCTG AAATTGAGAC CCGTGGGCCA 
AAGTTTAATC AGTGTGTGCA ACTTGGACAG GCTCTCCTGG AACGCAAGCA CAAGGACTCT 
GCAGAGATTA AAGAGAAGCT GATGCAACTG GTAGAGAAGA GAAAAGAGAT GATGCTCAAA 
TGGGATGATC GATGGGACTG GCTCAGACTT TTACTGGAGG TATGTCAGTT TGCTCGAGAT 
GCTTCTGTGG CAGAGGCATG GTTGATTGCC CAGGAGCCAT ATGTGGCCAG CAAGGACGTG 
GGTCAGACAG TCGATGAGGT TGAAAAACTG CTCAAAAGGC ATGAAGCATT TGAAAAATCT 
ACTGCAACAT GGGAAGAGCG TTTCTCAGCA TTGGAACGTC TTACAACATT GGAGTTGTTG 
GAAATTCGGA AACAACAACA AGAAATACAA CAGTACAGAC AAGAGGCAGA GAAAGAAAGC 
AGGAGAGAGG ACACTGGTTT TGCAGAGGAG TCTTCACAAC TTTACCCTAC AGAAGAACAG 
TCTCTGTCTG GTTTAGGAGT AATAGAGCCT AGTTCTGGTG GAGTTGATGG CACAGCAGGG 
GAATCCACTG TGCCTTTAAT CTCTGAGATG CAGGAAAGTG TCTCACTAGA GCTGGACCCT 
TCCACATCCA TTCAAGTTAC CAAAGAGACA GAAAAAGCTG CCTCGCTTCC TACAGAATCT 
ACACAAGTTC AACCAGTCCT GATGGAGGGA ACTCTAGCCC GCAAGCATGA GTTGGAGGGA 
CCTAACAAGA AGGCACCCAA CAGGTCCTGG AACAATCTGT ATTGTGTGCT TAAACCTGGA 
CACCTCTCTA TCTACAAGGA TGCCAAGAGC TTTAGTCACA GTGTAACCTT CCATGGTGAG 
GAACCCCTGA CCCTCACCAA TTCTTCATGC GAGATCTTGA CCAACTATAA GAAAAAGAAG 
CAGGTGTTCA AACTCCGACT TGGAGATGGA AGTGAATATC TGTTCCAGTG TAAAGACGAG 
GAGGAGCTTC AGAACTGGAC TCAGGCTATT GAGCAGGCTG CACAAGTTCA AATAGAAGAA 
CCAGTTGCAG GGCCATCAGG AGTGAAGGCA CTAAGCCTAC CTCCACCTTC CTCCACGACT 
CTAGAGGTTC CCTCAACGAA GAAGGACAAG GAAAAGAGGT TTAGTCTGTT TGCTAAGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005158849.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158906.1 Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.

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CloneID	ODa22741
Clone ID Related Accession (Same CDS sequence)	XM_005158849.3 , XM_005158849.4
Accession Version	XM_005158849.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, erythrocyte isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158849.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCC CCTGTTTGTG TCGTGCGGTG CCCGTGAGCC AGACCCGGCT GAGGGAGGTC 
AAACTTAGCT TGAAGACCAG CAGAGAGACT ATTGCAATCC GCTCTACCCC AGTCGTCCGA 
GAAGTCAGCA GCCGGCAGTA TACTACAGTT AAAGATGTCC CAACCCGGAC CAATGCACCA 
GGCAGTCGGA GCAGGAGCAC CCCACCAGTT TACAGGGCCC AGAGCAAGAG AGGGGCCAAA 
AACAACAGTC CTCATATTGG TGAACCTGAG CACCTGCCGG AGAGAAATGG AACTTTATTG 
GGGATCACAC CTAAACTGGC ACCCATGACT CTCACTTCAA TCTCCTCAAC CTCATCATCA 
TCATCTTCCT GCACCCCAAG GGGCCATCGG CCTTCACCTC TGCGATCCTC ACCATCTTGT 
CCCCTATCCC CGTCCCGGGG TATGAGACGA GCACGCTGGT TGAGCTACAG TGCTTCCAAC 
ATTTGCTTCA ATAATTCCAT TCTAGACGGC CGCTTCAGAC AACTGCAAGA TGAGCGAGAG 
GCTGTCCAGA AGAAGACTTT TACAAAATGG GTAAACTCCA TACTAGCCAG AGTCAGTTGC 
CGAATCTCAG ATCTCTATCT AGACCTGCGG GATGGGAGAA TGCTCATCAA ACTATTGGAA 
GTTCTGTCAG GGGAGAGATT GCCCAAGCCG ACCAAGGGCC GAATGCGTAT CCATTGTCTG 
GAGAATGTAG ACAAAGCTCT ACAGTTTCTA AAGGAACAGA AGGTGCACTT AGAGAACATG 
GGATCACATG ATATTGTAGA TGGGAACCAC CGGCTCATCC TGGGTCTCAT CTGGACCATC 
ATTCTTCGCT TCCAGATTCA AGATATCATA GTAGAAACAG GACAAGCTGA TCAGACGGGC 
CGGCAAGAGA CTCGCTCAGC CAAAGATGCA CTGCTCCTCT GGTGCCAGAT GAAGACTGCA 
GGGTACCCAA ACATAAATAT CACCAATTTC ACCACGAGCT GGAAAGACGG AATGGCTTTT 
AATGCTCTCA TTCACAAGCA CAGGCCAGAT CTGGTGGACT ATGGCAATCT AAAGAGGTCC 
AATCCCACAC ACAACCTGCA ACAGGCTTTT AATGTAGCTG AAAAGAAGCT TGGTGTCACC 
AAGTTATTGG ACCCAGAAGA TGTGTTCACT GAGAATCCAG ATGAGAAGTC CATAATCACA 
TATGTTGTTG CATTTTACCA CTATTTCTCC AAGATGAAAG CTCTGGCAGT TGAGGGCAAG 
CGAATTGGAA AGGTGCTGGA CCAGGCCATT GAAACTGAGA AGATGATTGA GAAGTATGAG 
ACACTGTCAT CGGACCTGCT AACATGGATT GAGCAAACCA TCGTAGTGCT AAACAACCGC 
AAGCTCGCTA ACTCCCTTAC AGGCGTCCAA CAGCAACTGC AGGCTTTCAA CTCTTACCGT 
ACTGTGGAGA AACCACCAAA GTTTCAGGAG AAAGGAAACT TGGAAGTACT GCTGTTTACC 
ATCCAGAGCA GAATGAGGGC CAACAATCAG AAAGTCTACA CACCTAAAGA GGGTGCATTG 
GTGTCAGATA TCAACAAGGC TTGGGAGAGG TTGGAGAAGG CAGAATATGA TCGTGAGCGG 
GTCTTAAGAG ATGAACTCAT TAGGCAAGAG AAGCTGGAAC AGATGGCTCG TCGCTTTGAC 
AGAAAGGCAG CCATGAGAGA AACCTGGCTT ATGGAGAATC AAAGACTGGT TTCACAGGAC 
AACTTTGGCT ATGACTTACC AGCAGTGGAG GCAGCTAAGA AGAAACATGA TGCTATAGAG 
ACAGACATTG CCGCATATGA AGAACGTGTT CAGGCACTGG TTGCCCTGTC TAAAGAACTG 
GAGTCAGAAA GATACCATGA TGCTAAACGC ATTGATGCTA GAAAGGACAA TATCTTGAGG 
CTGTGGGATT ACCTACAGGA GCTCCTAAAA GCTCGCAGAG GTCGCCTAGA CAAGAACCTC 
ACCCTTCAGA GAATTTTCCA GGAAATGCTC CACATCATCA GCTGGATGGA TGAGATGAAG 
GGTCGTCTGT TGTCCCCAGA CTTTGGAAAA CACTTATTGG AAGTGGAAGA CTTGTTGCAG 
AAACATTCAT TGGTAGAGGC TGATATAGCA GTGCAGGCAG AAAGAGTGAA ATCAGCCAAT 
GCAGCTGCTC TTAAATTTGC TAATGGTGAC AGTTATAAAC CATGTGACCC CCAAGTTATC 
CGTGATCGGG TACAGCATTT GGACCTGTGC TACCAGGAGT TGTGTGCACT GGCAGCTCAA 
AGAAAAGCAC GCCTTGAGCA GTCACGGCGC CTTTGGAACT TTTTATGGGA GATAGCAGAG 
CTGGAAAGCT GGATTCGTGA AAGGGAGCAA ATATTTTCCT CTCTTGACTA TGGTAAGGAC 
CTGACCAGTG TGTTGATACT GCAAAGCAAA CACAGTGTCT TTGAGGATGA GCTTGCAGCA 
AGACGGGACA ACTTGAAGCA GGTGATGGAC GAGGGAGAGA GCATGATCCA GATCAAGCAC 
TTGGGTTCCC CCAAAGTGCA GCAACGCATG AATGATGTAC AAAGACAGTG GCAACAACTG 
GAGGAACTGG CTGCATTCCG TAAACAGAAT CTGCAAGACA CCCAACGTTT CTTCCAGTTT 
CAGGGTGATG CTGATGACCT CAAGGCTTGG CTGGTCGATG CAATGAGACA GATGAGCAGT 
GACGATGTTG GGCATGATGA GTACACTACA CAGCGACTGC TCAAGAAACA CCGTGACTTA 
AGAGATGAGG CTGCTAAGAA TGGAGCCACC ATTGATGCTC TATCCAAACA GGCCAATGCA 
CTTCCTGAAG AACTACGGAA TACCCCTGAC ATCCAGGGCC GTCTGAATGA TATACGGGAT 
ATGTATATTG AGCTTCTGAC CCTCTCTGAT TTGAGGCAGA AGAAACTGGA TGACACCATG 
GCTCTTTACA CTATCTTCAG TGAGACAGAT GCTTGTGAGC TCTGGATGGG CCAGAAAGAG 
ACCTGGCTGG TTGGGTTAGA GACACCTGAG AATTTGGAAG ATCTAGAAAT TGTACAAAAC 
AGGCTAAGCA TTCTTGCTCA AGAAATGGGT AATATGCAGA CTCGAGTTGA TAACGTTAAT 
AAAGCAGCCA AGCAACTTGA AGACAGCAGA CATCCACAAA CAAAACAAGT GAAAGACTGT 
CAAATTCGTC TCAATAAGAG ATGGGAGGCT TTTAAAGCAA TGGTCGAAGA TAAGAAACAT 
AGGGTAGATT CTGCTCTTAG CCTACATAAC TATGATCTTG ATTGTGATGA GACAGAATCA 
TGGATAAAAG AAAAGACGAG AGTCATTGAG TCTACACAGG ATCTTGGGAA TGACCTTGCT 
GCTGTCATTA CTATTCAAAG GAAGCTTTTT GGCATGGAAA GAGACCTTGC TGCCATCCAA 
GATAAGTTGG ATTCCCTGCG TGACGAGGCC CAGAAGCTTG TAAAGGAGCA CCCAGAAAAT 
GCTTCTGATA TACTTGCCAG ACAGGAAGAG TTGGATGCAG CATGGGACAC CTTGAAACGT 
ACCCTGAAAG ACCGTGAAGA CTCTCTTGGG GAGGTCAGCA AGCTGCAGAC CTTCTTACAG 
GACATGGACG ATTTTCAAGC TTGGTTGTTC AAGTCACAGA AGGCTGTTGC TTCTGAAGAT 
ATGCCAGATG GTTTACCAGA GGCTGAGCAA CTCCTGAATC TCCATGATGC TTTGAGGCAT 
GATATGGATG GCCATGAGGA AGACTACCAT CGTGTGAAAG ACACAGGAGC TGCTGTTATT 
CAAGGTCAAG AAGATGATCC TCAGTACCAG CAACTGGAAC AGAGGCTGGA GGGTCTTGAT 
AAGGGCTGGG GTGAGCTTCA CAAAATGTGG GACAGTCGCA AGAACTTCCT AGATCAGGGT 
CTCGGTTTCC AACAATTCAT GAGAGATGCC AAACAGGCTG ATGCCATCTT AAATAATCAG 
GAATACACTC TAGCCCACGT AGACAAGCCT GACACCCTTG ATGGAGCAGA GAAGGCCTTG 
AAGAAACATG AGGACTTTGT TACCACCATG GATGCTAATA AGGAGAAGAT ACTTAGCACA 
CTGGAGACTG GTCAGAGGCT GGTGGATAGT GAAAACCTTT ACTCTGGAAA AGTGAAAGAC 
AAGATGAGTT CAATCGAGGA AAGGAACAAA AAGAACCAAG ACAAAGCCAA GGAAGTCTCA 
GGGAAACTTA AAGACAACAG GGAACTTCAG CACTTCCTTC AAAACACTCA GGATCTTACA 
TTGTGGATTA ATGAGAAAAT GCTGACTGCA CAAGATACAT CATATGATGA GGCTCGTAAC 
CTTCATAGCA AGTGGCAAAA ACACCAAGCT TTCATGGCAG AACTAGCATC AAACAAGGAT 
TGGCTTCATA ACATTGATAA GGAGGGACAG GAACTGATGG AATCCAAGCC TGAGTTTGAG 
CCTATTGTTA AGGATCGCCT TGCCAAGCTT CATGAACTAT GGGATAAGCT GGAGAGCACC 
ACACAAGAAA AGGCTCGGCT ACTGTTTGAT GCAAACCGTT CTGAGCTGTT CGATCAGAGC 
CTAGCTGACC TAAAGAAATG GTTAGCTGAA TTGCAGCAGC AACTTCAGGG TGATGTGGAA 
GAAGAAGTTA AGGATCTTAC CAGTGCCAAC ATCCTGCTAA AGAAACATCA GATAACAGAG 
AATCAGGTTC GGGACCGTGC ACGAGAGCTT GAAGAACTTC AGGAGGCTGT AGAGCAGCAT 
GGTTCTCTTA GAGAGGACCA GCCTGAGCTT GAGATTGAGC AACAAAATCT TCAGAGAGAT 
TTCCAGAAGC TCCTCACACC TTTATCACAA CGCAAGGGAA AGCTGGAGGC TGCTAAGGCA 
GTGCACCAGT TCTTTAGAGA TCTTGCTGAT GAAATACTCT GGGTTAATGA AAGGTTACCA 
ATGGCTATGT CAGATGATCA TGGTAACAAT CTTCAGACTG TCCAACTTTT GCTTAAGAAG 
AACCAATCCT TACAAAAAGA GATTGATGGT CATCAACCAC GCATTGATGA AGTGTTAGAG 
CGGGGTCGGC GAATGGCAGC AGCAGCAGAG GGCAGTCCAG AGGAAGAACG CATGTCTGAG 
GAGATGAAGA AGCTTCAGGT GGTGTGGGCA CAGCTACAGG AAGAGATGGC AAAACGAAGA 
GAACGGCTTT ATGGCTCTAA TGAGGCCCAA CAGTATTACA ATGATGCAGA TGATTCTGAG 
GCCTGGATAG GTGAACAAGA ACTCTACATG ATTGCTGATG AGATGGCAAA GGATGAACAG 
AGTGCTATGA TCATGCTGAA GCGGCACCTG GTTCTTAAGC AGACAGTGGA TGACTATGCA 
TATTCCATCC AGCAATTGGC AGACCGTGCC CAGAAGATGC TGGCTGAAGA GCACCCAGAT 
GGTGAGGCTA TCATCCGAAG ACAAGGACAA GTTGATAAGC AGTATGCTGG GCTGAAGGAG 
CTGGCAGAAG ACCGCAAAAA GAAGCTGGAC CACACATATC ACCATTTCCT GCTGAGTCGG 
GAAGTGGAGG ATCTAGAGCA GTGGATTGCA GAGCGAGATG TAGTGGCTTC ATCACAAGAA 
ATGGGCCAAG ACCTTGATCA TGTGACGATC TTGCGAGACA AGTTCAGGGA GTTTGCACGG 
GAAACAGGGA CAGTGGGTCA AGAACGTGTA GATACTGTGA ACCGGATCAT AGATGAACTA 
ATTGAAGGAG GCCACAGTGA GTCAGCGACA TTGGCCGAGT GGAAGGATGG AGTAAATGAG 
AGTTGGGCAG ATTTGCTGGA GCTCATTGAC ACTCGTGCCC AGCTCCTCAC ATCCTCTTAT 
GACCTCCTTA AGTATTTTTA TGATGGAAAA GAGCTGGTTG GTCACATTGA GGAAAAGAAG 
AATGAACTTC CTGAAGACTT GGGAGAAGAC TTCAGCAAAG CAGAATCATT CCACCGAATG 
CATGCTGCAT TTGAGAGGGA TATCAGCTCC TTGGGCAAAC AGGTAAAACA GTTCCAGGAG 
ACAGCAGCTA GATTGCATGC CCAGTATGCA GGAGATCAGG CTACTGCCAT TCAAGCAACT 
GAAAAAGAGG TTGTTGAGGC CTGGAAGGGT CTGTTGGATG CTTGTGCGGG TAGGAGGAAA 
CAGCTGGAGG AGACAGCAGA CAAATTCCGC TTTTTCACCA TGGTGAGAGA CCTAATGGCC 
TGGATGGAAA GCATCCTTCA ACAGATAGAG ACACAGGAAA AACCAAGAGA CGTCTCCTCA 
GTGGAGCTTC TGATGAAGTA TCATCAAGGG ATCCGTGCTG AAATTGAGAC CCGTGGGCCA 
AAGTTTAATC AGTGTGTGCA ACTTGGACAG GCTCTCCTGG AACGCAAGCA CAAGGACTCT 
GCAGAGATTA AAGAGAAGCT GATGCAACTG GTAGAGAAGA GAAAAGAGAT GATGCTCAAA 
TGGGATGATC GATGGGACTG GCTCAGACTT TTACTGGAGG TATGTCAGTT TGCTCGAGAT 
GCTTCTGTGG CAGAGGCATG GTTGATTGCC CAGGAGCCAT ATGTGGCCAG CAAGGACGTG 
GGTCAGACAG TCGATGAGGT TGAAAAACTG CTCAAAAGGC ATGAAGCATT TGAAAAATCT 
ACTGCAACAT GGGAAGAGCG TTTCTCAGCA TTGGAACGTC TTACAACATT GGAGTTGTTG 
GAAATTCGGA AACAACAACA AGAAATACAA CAGTACAGAC AAGAGGCAGA GAAAGAAAGC 
AGGAGAGAGG ACACTGGTTT TGCAGAGGAG TCTTCACAAC TTTACCCTAC AGAAGAACAG 
TCTCTGTCTG GTTTAGGAGT AATAGAGCCT AGTTCTGGTG GAGTTGATGG CACAGCAGGG 
GAATCCACTG TGCCTTTAAT CTCTGAGATG CAGGAAAGTG TCTCACTAGA GCTGGACCCT 
TCCACATCCA TTCAAGTTAC CAAAGAGACA GAAAAAGCTG CCTCGCTTCC TACAGAATCT 
ACACAAGTTC AACCAGTCCT GATGGAGGGA ACTCTAGCCC GCAAGCATGA GTTGGAGGGA 
CCTAACAAGA AGGCACCCAA CAGGTCCTGG AACAATCTGT ATTGTGTGCT TAAACCTGGA 
CACCTCTCTA TCTACAAGGA TGCCAAGAGC TTTAGTCACA GTGTAACCTT CCATGGTGAG 
GAACCCCTGA CCCTCACCAA TTCTTCATGC GAGATCTTGA CCAACTATAA GAAAAAGAAG 
CAGGTGTTCA AACTCCGACT TGGAGATGGA AGTGAATATC TGTTCCAGTG TAAAGACGAG 
GAGGAGCTTC AGAACTGGAC TCAGGCTATT GAGCAGGCTG CACAAGTTCA AATAGAAGAA 
CCAGTTGCAG GGCCATCAGG AGTGAAGGCA CTAAGCCTAC CTCCACCTTC CTCCACGACT 
CTAGAGGTTC CCTCAACGAA GAAGGACAAG GAAAAGAGGT TTAGTCTGTT TGCTAAGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158906.1
CDS	73..7518
Translation	MSSPCLCRAVPVSQTRLREVKLSLKTSRETIAIRSTPVVREVSSRQYTTVKDVPTRTNAPGSRSRSTPPVYRAQSKRGAKNNSPHIGEPEHLPERNGTLLGITPKLAPMTLTSISSTSSSSSSCTPRGHRPSPLRSSPSCPLSPSRGMRRARWLSYSASNICFNNSILDGRFRQLQDEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQVVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	XM_005158849.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158849.4

ORF Insert Sequence:

ATGAGCAGCC CCTGTTTGTG TCGTGCGGTG CCCGTGAGCC AGACCCGGCT GAGGGAGGTC 
AAACTTAGCT TGAAGACCAG CAGAGAGACT ATTGCAATCC GCTCTACCCC AGTCGTCCGA 
GAAGTCAGCA GCCGGCAGTA TACTACAGTT AAAGATGTCC CAACCCGGAC CAATGCACCA 
GGCAGTCGGA GCAGGAGCAC CCCACCAGTT TACAGGGCCC AGAGCAAGAG AGGGGCCAAA 
AACAACAGTC CTCATATTGG TGAACCTGAG CACCTGCCGG AGAGAAATGG AACTTTATTG 
GGGATCACAC CTAAACTGGC ACCCATGACT CTCACTTCAA TCTCCTCAAC CTCATCATCA 
TCATCTTCCT GCACCCCAAG GGGCCATCGG CCTTCACCTC TGCGATCCTC ACCATCTTGT 
CCCCTATCCC CGTCCCGGGG TATGAGACGA GCACGCTGGT TGAGCTACAG TGCTTCCAAC 
ATTTGCTTCA ATAATTCCAT TCTAGACGGC CGCTTCAGAC AACTGCAAGA TGAGCGAGAG 
GCTGTCCAGA AGAAGACTTT TACAAAATGG GTAAACTCCA TACTAGCCAG AGTCAGTTGC 
CGAATCTCAG ATCTCTATCT AGACCTGCGG GATGGGAGAA TGCTCATCAA ACTATTGGAA 
GTTCTGTCAG GGGAGAGATT GCCCAAGCCG ACCAAGGGCC GAATGCGTAT CCATTGTCTG 
GAGAATGTAG ACAAAGCTCT ACAGTTTCTA AAGGAACAGA AGGTGCACTT AGAGAACATG 
GGATCACATG ATATTGTAGA TGGGAACCAC CGGCTCATCC TGGGTCTCAT CTGGACCATC 
ATTCTTCGCT TCCAGATTCA AGATATCATA GTAGAAACAG GACAAGCTGA TCAGACGGGC 
CGGCAAGAGA CTCGCTCAGC CAAAGATGCA CTGCTCCTCT GGTGCCAGAT GAAGACTGCA 
GGGTACCCAA ACATAAATAT CACCAATTTC ACCACGAGCT GGAAAGACGG AATGGCTTTT 
AATGCTCTCA TTCACAAGCA CAGGCCAGAT CTGGTGGACT ATGGCAATCT AAAGAGGTCC 
AATCCCACAC ACAACCTGCA ACAGGCTTTT AATGTAGCTG AAAAGAAGCT TGGTGTCACC 
AAGTTATTGG ACCCAGAAGA TGTGTTCACT GAGAATCCAG ATGAGAAGTC CATAATCACA 
TATGTTGTTG CATTTTACCA CTATTTCTCC AAGATGAAAG CTCTGGCAGT TGAGGGCAAG 
CGAATTGGAA AGGTGCTGGA CCAGGCCATT GAAACTGAGA AGATGATTGA GAAGTATGAG 
ACACTGTCAT CGGACCTGCT AACATGGATT GAGCAAACCA TCGTAGTGCT AAACAACCGC 
AAGCTCGCTA ACTCCCTTAC AGGCGTCCAA CAGCAACTGC AGGCTTTCAA CTCTTACCGT 
ACTGTGGAGA AACCACCAAA GTTTCAGGAG AAAGGAAACT TGGAAGTACT GCTGTTTACC 
ATCCAGAGCA GAATGAGGGC CAACAATCAG AAAGTCTACA CACCTAAAGA GGGTGCATTG 
GTGTCAGATA TCAACAAGGC TTGGGAGAGG TTGGAGAAGG CAGAATATGA TCGTGAGCGG 
GTCTTAAGAG ATGAACTCAT TAGGCAAGAG AAGCTGGAAC AGATGGCTCG TCGCTTTGAC 
AGAAAGGCAG CCATGAGAGA AACCTGGCTT ATGGAGAATC AAAGACTGGT TTCACAGGAC 
AACTTTGGCT ATGACTTACC AGCAGTGGAG GCAGCTAAGA AGAAACATGA TGCTATAGAG 
ACAGACATTG CCGCATATGA AGAACGTGTT CAGGCACTGG TTGCCCTGTC TAAAGAACTG 
GAGTCAGAAA GATACCATGA TGCTAAACGC ATTGATGCTA GAAAGGACAA TATCTTGAGG 
CTGTGGGATT ACCTACAGGA GCTCCTAAAA GCTCGCAGAG GTCGCCTAGA CAAGAACCTC 
ACCCTTCAGA GAATTTTCCA GGAAATGCTC CACATCATCA GCTGGATGGA TGAGATGAAG 
GGTCGTCTGT TGTCCCCAGA CTTTGGAAAA CACTTATTGG AAGTGGAAGA CTTGTTGCAG 
AAACATTCAT TGGTAGAGGC TGATATAGCA GTGCAGGCAG AAAGAGTGAA ATCAGCCAAT 
GCAGCTGCTC TTAAATTTGC TAATGGTGAC AGTTATAAAC CATGTGACCC CCAAGTTATC 
CGTGATCGGG TACAGCATTT GGACCTGTGC TACCAGGAGT TGTGTGCACT GGCAGCTCAA 
AGAAAAGCAC GCCTTGAGCA GTCACGGCGC CTTTGGAACT TTTTATGGGA GATAGCAGAG 
CTGGAAAGCT GGATTCGTGA AAGGGAGCAA ATATTTTCCT CTCTTGACTA TGGTAAGGAC 
CTGACCAGTG TGTTGATACT GCAAAGCAAA CACAGTGTCT TTGAGGATGA GCTTGCAGCA 
AGACGGGACA ACTTGAAGCA GGTGATGGAC GAGGGAGAGA GCATGATCCA GATCAAGCAC 
TTGGGTTCCC CCAAAGTGCA GCAACGCATG AATGATGTAC AAAGACAGTG GCAACAACTG 
GAGGAACTGG CTGCATTCCG TAAACAGAAT CTGCAAGACA CCCAACGTTT CTTCCAGTTT 
CAGGGTGATG CTGATGACCT CAAGGCTTGG CTGGTCGATG CAATGAGACA GATGAGCAGT 
GACGATGTTG GGCATGATGA GTACACTACA CAGCGACTGC TCAAGAAACA CCGTGACTTA 
AGAGATGAGG CTGCTAAGAA TGGAGCCACC ATTGATGCTC TATCCAAACA GGCCAATGCA 
CTTCCTGAAG AACTACGGAA TACCCCTGAC ATCCAGGGCC GTCTGAATGA TATACGGGAT 
ATGTATATTG AGCTTCTGAC CCTCTCTGAT TTGAGGCAGA AGAAACTGGA TGACACCATG 
GCTCTTTACA CTATCTTCAG TGAGACAGAT GCTTGTGAGC TCTGGATGGG CCAGAAAGAG 
ACCTGGCTGG TTGGGTTAGA GACACCTGAG AATTTGGAAG ATCTAGAAAT TGTACAAAAC 
AGGCTAAGCA TTCTTGCTCA AGAAATGGGT AATATGCAGA CTCGAGTTGA TAACGTTAAT 
AAAGCAGCCA AGCAACTTGA AGACAGCAGA CATCCACAAA CAAAACAAGT GAAAGACTGT 
CAAATTCGTC TCAATAAGAG ATGGGAGGCT TTTAAAGCAA TGGTCGAAGA TAAGAAACAT 
AGGGTAGATT CTGCTCTTAG CCTACATAAC TATGATCTTG ATTGTGATGA GACAGAATCA 
TGGATAAAAG AAAAGACGAG AGTCATTGAG TCTACACAGG ATCTTGGGAA TGACCTTGCT 
GCTGTCATTA CTATTCAAAG GAAGCTTTTT GGCATGGAAA GAGACCTTGC TGCCATCCAA 
GATAAGTTGG ATTCCCTGCG TGACGAGGCC CAGAAGCTTG TAAAGGAGCA CCCAGAAAAT 
GCTTCTGATA TACTTGCCAG ACAGGAAGAG TTGGATGCAG CATGGGACAC CTTGAAACGT 
ACCCTGAAAG ACCGTGAAGA CTCTCTTGGG GAGGTCAGCA AGCTGCAGAC CTTCTTACAG 
GACATGGACG ATTTTCAAGC TTGGTTGTTC AAGTCACAGA AGGCTGTTGC TTCTGAAGAT 
ATGCCAGATG GTTTACCAGA GGCTGAGCAA CTCCTGAATC TCCATGATGC TTTGAGGCAT 
GATATGGATG GCCATGAGGA AGACTACCAT CGTGTGAAAG ACACAGGAGC TGCTGTTATT 
CAAGGTCAAG AAGATGATCC TCAGTACCAG CAACTGGAAC AGAGGCTGGA GGGTCTTGAT 
AAGGGCTGGG GTGAGCTTCA CAAAATGTGG GACAGTCGCA AGAACTTCCT AGATCAGGGT 
CTCGGTTTCC AACAATTCAT GAGAGATGCC AAACAGGCTG ATGCCATCTT AAATAATCAG 
GAATACACTC TAGCCCACGT AGACAAGCCT GACACCCTTG ATGGAGCAGA GAAGGCCTTG 
AAGAAACATG AGGACTTTGT TACCACCATG GATGCTAATA AGGAGAAGAT ACTTAGCACA 
CTGGAGACTG GTCAGAGGCT GGTGGATAGT GAAAACCTTT ACTCTGGAAA AGTGAAAGAC 
AAGATGAGTT CAATCGAGGA AAGGAACAAA AAGAACCAAG ACAAAGCCAA GGAAGTCTCA 
GGGAAACTTA AAGACAACAG GGAACTTCAG CACTTCCTTC AAAACACTCA GGATCTTACA 
TTGTGGATTA ATGAGAAAAT GCTGACTGCA CAAGATACAT CATATGATGA GGCTCGTAAC 
CTTCATAGCA AGTGGCAAAA ACACCAAGCT TTCATGGCAG AACTAGCATC AAACAAGGAT 
TGGCTTCATA ACATTGATAA GGAGGGACAG GAACTGATGG AATCCAAGCC TGAGTTTGAG 
CCTATTGTTA AGGATCGCCT TGCCAAGCTT CATGAACTAT GGGATAAGCT GGAGAGCACC 
ACACAAGAAA AGGCTCGGCT ACTGTTTGAT GCAAACCGTT CTGAGCTGTT CGATCAGAGC 
CTAGCTGACC TAAAGAAATG GTTAGCTGAA TTGCAGCAGC AACTTCAGGG TGATGTGGAA 
GAAGAAGTTA AGGATCTTAC CAGTGCCAAC ATCCTGCTAA AGAAACATCA GATAACAGAG 
AATCAGGTTC GGGACCGTGC ACGAGAGCTT GAAGAACTTC AGGAGGCTGT AGAGCAGCAT 
GGTTCTCTTA GAGAGGACCA GCCTGAGCTT GAGATTGAGC AACAAAATCT TCAGAGAGAT 
TTCCAGAAGC TCCTCACACC TTTATCACAA CGCAAGGGAA AGCTGGAGGC TGCTAAGGCA 
GTGCACCAGT TCTTTAGAGA TCTTGCTGAT GAAATACTCT GGGTTAATGA AAGGTTACCA 
ATGGCTATGT CAGATGATCA TGGTAACAAT CTTCAGACTG TCCAACTTTT GCTTAAGAAG 
AACCAATCCT TACAAAAAGA GATTGATGGT CATCAACCAC GCATTGATGA AGTGTTAGAG 
CGGGGTCGGC GAATGGCAGC AGCAGCAGAG GGCAGTCCAG AGGAAGAACG CATGTCTGAG 
GAGATGAAGA AGCTTCAGGT GGTGTGGGCA CAGCTACAGG AAGAGATGGC AAAACGAAGA 
GAACGGCTTT ATGGCTCTAA TGAGGCCCAA CAGTATTACA ATGATGCAGA TGATTCTGAG 
GCCTGGATAG GTGAACAAGA ACTCTACATG ATTGCTGATG AGATGGCAAA GGATGAACAG 
AGTGCTATGA TCATGCTGAA GCGGCACCTG GTTCTTAAGC AGACAGTGGA TGACTATGCA 
TATTCCATCC AGCAATTGGC AGACCGTGCC CAGAAGATGC TGGCTGAAGA GCACCCAGAT 
GGTGAGGCTA TCATCCGAAG ACAAGGACAA GTTGATAAGC AGTATGCTGG GCTGAAGGAG 
CTGGCAGAAG ACCGCAAAAA GAAGCTGGAC CACACATATC ACCATTTCCT GCTGAGTCGG 
GAAGTGGAGG ATCTAGAGCA GTGGATTGCA GAGCGAGATG TAGTGGCTTC ATCACAAGAA 
ATGGGCCAAG ACCTTGATCA TGTGACGATC TTGCGAGACA AGTTCAGGGA GTTTGCACGG 
GAAACAGGGA CAGTGGGTCA AGAACGTGTA GATACTGTGA ACCGGATCAT AGATGAACTA 
ATTGAAGGAG GCCACAGTGA GTCAGCGACA TTGGCCGAGT GGAAGGATGG AGTAAATGAG 
AGTTGGGCAG ATTTGCTGGA GCTCATTGAC ACTCGTGCCC AGCTCCTCAC ATCCTCTTAT 
GACCTCCTTA AGTATTTTTA TGATGGAAAA GAGCTGGTTG GTCACATTGA GGAAAAGAAG 
AATGAACTTC CTGAAGACTT GGGAGAAGAC TTCAGCAAAG CAGAATCATT CCACCGAATG 
CATGCTGCAT TTGAGAGGGA TATCAGCTCC TTGGGCAAAC AGGTAAAACA GTTCCAGGAG 
ACAGCAGCTA GATTGCATGC CCAGTATGCA GGAGATCAGG CTACTGCCAT TCAAGCAACT 
GAAAAAGAGG TTGTTGAGGC CTGGAAGGGT CTGTTGGATG CTTGTGCGGG TAGGAGGAAA 
CAGCTGGAGG AGACAGCAGA CAAATTCCGC TTTTTCACCA TGGTGAGAGA CCTAATGGCC 
TGGATGGAAA GCATCCTTCA ACAGATAGAG ACACAGGAAA AACCAAGAGA CGTCTCCTCA 
GTGGAGCTTC TGATGAAGTA TCATCAAGGG ATCCGTGCTG AAATTGAGAC CCGTGGGCCA 
AAGTTTAATC AGTGTGTGCA ACTTGGACAG GCTCTCCTGG AACGCAAGCA CAAGGACTCT 
GCAGAGATTA AAGAGAAGCT GATGCAACTG GTAGAGAAGA GAAAAGAGAT GATGCTCAAA 
TGGGATGATC GATGGGACTG GCTCAGACTT TTACTGGAGG TATGTCAGTT TGCTCGAGAT 
GCTTCTGTGG CAGAGGCATG GTTGATTGCC CAGGAGCCAT ATGTGGCCAG CAAGGACGTG 
GGTCAGACAG TCGATGAGGT TGAAAAACTG CTCAAAAGGC ATGAAGCATT TGAAAAATCT 
ACTGCAACAT GGGAAGAGCG TTTCTCAGCA TTGGAACGTC TTACAACATT GGAGTTGTTG 
GAAATTCGGA AACAACAACA AGAAATACAA CAGTACAGAC AAGAGGCAGA GAAAGAAAGC 
AGGAGAGAGG ACACTGGTTT TGCAGAGGAG TCTTCACAAC TTTACCCTAC AGAAGAACAG 
TCTCTGTCTG GTTTAGGAGT AATAGAGCCT AGTTCTGGTG GAGTTGATGG CACAGCAGGG 
GAATCCACTG TGCCTTTAAT CTCTGAGATG CAGGAAAGTG TCTCACTAGA GCTGGACCCT 
TCCACATCCA TTCAAGTTAC CAAAGAGACA GAAAAAGCTG CCTCGCTTCC TACAGAATCT 
ACACAAGTTC AACCAGTCCT GATGGAGGGA ACTCTAGCCC GCAAGCATGA GTTGGAGGGA 
CCTAACAAGA AGGCACCCAA CAGGTCCTGG AACAATCTGT ATTGTGTGCT TAAACCTGGA 
CACCTCTCTA TCTACAAGGA TGCCAAGAGC TTTAGTCACA GTGTAACCTT CCATGGTGAG 
GAACCCCTGA CCCTCACCAA TTCTTCATGC GAGATCTTGA CCAACTATAA GAAAAAGAAG 
CAGGTGTTCA AACTCCGACT TGGAGATGGA AGTGAATATC TGTTCCAGTG TAAAGACGAG 
GAGGAGCTTC AGAACTGGAC TCAGGCTATT GAGCAGGCTG CACAAGTTCA AATAGAAGAA 
CCAGTTGCAG GGCCATCAGG AGTGAAGGCA CTAAGCCTAC CTCCACCTTC CTCCACGACT 
CTAGAGGTTC CCTCAACGAA GAAGGACAAG GAAAAGAGGT TTAGTCTGTT TGCTAAGAAG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005158850.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158907.1 Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.

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CloneID	ODa22742
Clone ID Related Accession (Same CDS sequence)	XM_005158850.3 , XM_005158851.4 , XM_005158850.2 , XM_005158851.3
Accession Version	XM_005158850.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, erythrocyte isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158850.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158907.1
CDS	344..7417
Translation	MATMTSTTDYDNAEITQQYSRINTRFELSDEELDNDNSSARLFERSRIKALADEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQVVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	XM_005158850.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158850.3

ORF Insert Sequence:

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005158851.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158908.1 Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X3, mRNA.

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CloneID	ODa22742
Clone ID Related Accession (Same CDS sequence)	XM_005158850.3 , XM_005158851.4 , XM_005158850.2 , XM_005158851.3
Accession Version	XM_005158851.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	spectrin beta chain, erythrocyte isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158851.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158908.1
CDS	340..7413
Translation	MATMTSTTDYDNAEITQQYSRINTRFELSDEELDNDNSSARLFERSRIKALADEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQVVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	XM_005158851.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158851.4

ORF Insert Sequence:

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005158850.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158907.1 Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.

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CloneID	ODa22742
Clone ID Related Accession (Same CDS sequence)	XM_005158850.3 , XM_005158851.4 , XM_005158850.2 , XM_005158851.3
Accession Version	XM_005158850.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	spectrin beta chain, erythrocyte isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158907.1
CDS	344..7417
Translation	MATMTSTTDYDNAEITQQYSRINTRFELSDEELDNDNSSARLFERSRIKALADEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQVVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	XM_005158850.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158850.2

ORF Insert Sequence:

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTB ( XM_005158851.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005158908.1 Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X3, mRNA.

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CloneID	ODa22742
Clone ID Related Accession (Same CDS sequence)	XM_005158850.3 , XM_005158851.4 , XM_005158850.2 , XM_005158851.3
Accession Version	XM_005158851.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	spectrin beta chain, erythrocyte isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158851.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158908.1
CDS	326..7399
Translation	MATMTSTTDYDNAEITQQYSRINTRFELSDEELDNDNSSARLFERSRIKALADEREAVQKKTFTKWVNSILARVSCRISDLYLDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQKVHLENMGSHDIVDGNHRLILGLIWTIILRFQIQDIIVETGQADQTGRQETRSAKDALLLWCQMKTAGYPNINITNFTTSWKDGMAFNALIHKHRPDLVDYGNLKRSNPTHNLQQAFNVAEKKLGVTKLLDPEDVFTENPDEKSIITYVVAFYHYFSKMKALAVEGKRIGKVLDQAIETEKMIEKYETLSSDLLTWIEQTIVVLNNRKLANSLTGVQQQLQAFNSYRTVEKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPKEGALVSDINKAWERLEKAEYDRERVLRDELIRQEKLEQMARRFDRKAAMRETWLMENQRLVSQDNFGYDLPAVEAAKKKHDAIETDIAAYEERVQALVALSKELESERYHDAKRIDARKDNILRLWDYLQELLKARRGRLDKNLTLQRIFQEMLHIISWMDEMKGRLLSPDFGKHLLEVEDLLQKHSLVEADIAVQAERVKSANAAALKFANGDSYKPCDPQVIRDRVQHLDLCYQELCALAAQRKARLEQSRRLWNFLWEIAELESWIREREQIFSSLDYGKDLTSVLILQSKHSVFEDELAARRDNLKQVMDEGESMIQIKHLGSPKVQQRMNDVQRQWQQLEELAAFRKQNLQDTQRFFQFQGDADDLKAWLVDAMRQMSSDDVGHDEYTTQRLLKKHRDLRDEAAKNGATIDALSKQANALPEELRNTPDIQGRLNDIRDMYIELLTLSDLRQKKLDDTMALYTIFSETDACELWMGQKETWLVGLETPENLEDLEIVQNRLSILAQEMGNMQTRVDNVNKAAKQLEDSRHPQTKQVKDCQIRLNKRWEAFKAMVEDKKHRVDSALSLHNYDLDCDETESWIKEKTRVIESTQDLGNDLAAVITIQRKLFGMERDLAAIQDKLDSLRDEAQKLVKEHPENASDILARQEELDAAWDTLKRTLKDREDSLGEVSKLQTFLQDMDDFQAWLFKSQKAVASEDMPDGLPEAEQLLNLHDALRHDMDGHEEDYHRVKDTGAAVIQGQEDDPQYQQLEQRLEGLDKGWGELHKMWDSRKNFLDQGLGFQQFMRDAKQADAILNNQEYTLAHVDKPDTLDGAEKALKKHEDFVTTMDANKEKILSTLETGQRLVDSENLYSGKVKDKMSSIEERNKKNQDKAKEVSGKLKDNRELQHFLQNTQDLTLWINEKMLTAQDTSYDEARNLHSKWQKHQAFMAELASNKDWLHNIDKEGQELMESKPEFEPIVKDRLAKLHELWDKLESTTQEKARLLFDANRSELFDQSLADLKKWLAELQQQLQGDVEEEVKDLTSANILLKKHQITENQVRDRARELEELQEAVEQHGSLREDQPELEIEQQNLQRDFQKLLTPLSQRKGKLEAAKAVHQFFRDLADEILWVNERLPMAMSDDHGNNLQTVQLLLKKNQSLQKEIDGHQPRIDEVLERGRRMAAAAEGSPEEERMSEEMKKLQVVWAQLQEEMAKRRERLYGSNEAQQYYNDADDSEAWIGEQELYMIADEMAKDEQSAMIMLKRHLVLKQTVDDYAYSIQQLADRAQKMLAEEHPDGEAIIRRQGQVDKQYAGLKELAEDRKKKLDHTYHHFLLSREVEDLEQWIAERDVVASSQEMGQDLDHVTILRDKFREFARETGTVGQERVDTVNRIIDELIEGGHSESATLAEWKDGVNESWADLLELIDTRAQLLTSSYDLLKYFYDGKELVGHIEEKKNELPEDLGEDFSKAESFHRMHAAFERDISSLGKQVKQFQETAARLHAQYAGDQATAIQATEKEVVEAWKGLLDACAGRRKQLEETADKFRFFTMVRDLMAWMESILQQIETQEKPRDVSSVELLMKYHQGIRAEIETRGPKFNQCVQLGQALLERKHKDSAEIKEKLMQLVEKRKEMMLKWDDRWDWLRLLLEVCQFARDASVAEAWLIAQEPYVASKDVGQTVDEVEKLLKRHEAFEKSTATWEERFSALERLTTLELLEIRKQQQEIQQYRQEAEKESRREDTGFAEESSQLYPTEEQSLSGLGVIEPSSGGVDGTAGESTVPLISEMQESVSLELDPSTSIQVTKETEKAASLPTESTQVQPVLMEGTLARKHELEGPNKKAPNRSWNNLYCVLKPGHLSIYKDAKSFSHSVTFHGEEPLTLTNSSCEILTNYKKKKQVFKLRLGDGSEYLFQCKDEEELQNWTQAIEQAAQVQIEEPVAGPSGVKALSLPPPSSTTLEVPSTKKDKEKRFSLFAKKK

Target ORF information:

RefSeq Version	XM_005158851.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio spectrin, beta, erythrocytic (sptb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158851.3

ORF Insert Sequence:

ATGGCAACTA TGACCTCCAC CACAGACTAT GACAACGCTG AAATCACTCA GCAGTACAGC 
CGTATCAACA CTCGCTTCGA ACTGTCTGAT GAAGAACTTG ACAATGACAA CAGCTCAGCC 
AGACTCTTTG AGCGCTCACG CATTAAAGCT CTTGCAGATG AGCGAGAGGC TGTCCAGAAG 
AAGACTTTTA CAAAATGGGT AAACTCCATA CTAGCCAGAG TCAGTTGCCG AATCTCAGAT 
CTCTATCTAG ACCTGCGGGA TGGGAGAATG CTCATCAAAC TATTGGAAGT TCTGTCAGGG 
GAGAGATTGC CCAAGCCGAC CAAGGGCCGA ATGCGTATCC ATTGTCTGGA GAATGTAGAC 
AAAGCTCTAC AGTTTCTAAA GGAACAGAAG GTGCACTTAG AGAACATGGG ATCACATGAT 
ATTGTAGATG GGAACCACCG GCTCATCCTG GGTCTCATCT GGACCATCAT TCTTCGCTTC 
CAGATTCAAG ATATCATAGT AGAAACAGGA CAAGCTGATC AGACGGGCCG GCAAGAGACT 
CGCTCAGCCA AAGATGCACT GCTCCTCTGG TGCCAGATGA AGACTGCAGG GTACCCAAAC 
ATAAATATCA CCAATTTCAC CACGAGCTGG AAAGACGGAA TGGCTTTTAA TGCTCTCATT 
CACAAGCACA GGCCAGATCT GGTGGACTAT GGCAATCTAA AGAGGTCCAA TCCCACACAC 
AACCTGCAAC AGGCTTTTAA TGTAGCTGAA AAGAAGCTTG GTGTCACCAA GTTATTGGAC 
CCAGAAGATG TGTTCACTGA GAATCCAGAT GAGAAGTCCA TAATCACATA TGTTGTTGCA 
TTTTACCACT ATTTCTCCAA GATGAAAGCT CTGGCAGTTG AGGGCAAGCG AATTGGAAAG 
GTGCTGGACC AGGCCATTGA AACTGAGAAG ATGATTGAGA AGTATGAGAC ACTGTCATCG 
GACCTGCTAA CATGGATTGA GCAAACCATC GTAGTGCTAA ACAACCGCAA GCTCGCTAAC 
TCCCTTACAG GCGTCCAACA GCAACTGCAG GCTTTCAACT CTTACCGTAC TGTGGAGAAA 
CCACCAAAGT TTCAGGAGAA AGGAAACTTG GAAGTACTGC TGTTTACCAT CCAGAGCAGA 
ATGAGGGCCA ACAATCAGAA AGTCTACACA CCTAAAGAGG GTGCATTGGT GTCAGATATC 
AACAAGGCTT GGGAGAGGTT GGAGAAGGCA GAATATGATC GTGAGCGGGT CTTAAGAGAT 
GAACTCATTA GGCAAGAGAA GCTGGAACAG ATGGCTCGTC GCTTTGACAG AAAGGCAGCC 
ATGAGAGAAA CCTGGCTTAT GGAGAATCAA AGACTGGTTT CACAGGACAA CTTTGGCTAT 
GACTTACCAG CAGTGGAGGC AGCTAAGAAG AAACATGATG CTATAGAGAC AGACATTGCC 
GCATATGAAG AACGTGTTCA GGCACTGGTT GCCCTGTCTA AAGAACTGGA GTCAGAAAGA 
TACCATGATG CTAAACGCAT TGATGCTAGA AAGGACAATA TCTTGAGGCT GTGGGATTAC 
CTACAGGAGC TCCTAAAAGC TCGCAGAGGT CGCCTAGACA AGAACCTCAC CCTTCAGAGA 
ATTTTCCAGG AAATGCTCCA CATCATCAGC TGGATGGATG AGATGAAGGG TCGTCTGTTG 
TCCCCAGACT TTGGAAAACA CTTATTGGAA GTGGAAGACT TGTTGCAGAA ACATTCATTG 
GTAGAGGCTG ATATAGCAGT GCAGGCAGAA AGAGTGAAAT CAGCCAATGC AGCTGCTCTT 
AAATTTGCTA ATGGTGACAG TTATAAACCA TGTGACCCCC AAGTTATCCG TGATCGGGTA 
CAGCATTTGG ACCTGTGCTA CCAGGAGTTG TGTGCACTGG CAGCTCAAAG AAAAGCACGC 
CTTGAGCAGT CACGGCGCCT TTGGAACTTT TTATGGGAGA TAGCAGAGCT GGAAAGCTGG 
ATTCGTGAAA GGGAGCAAAT ATTTTCCTCT CTTGACTATG GTAAGGACCT GACCAGTGTG 
TTGATACTGC AAAGCAAACA CAGTGTCTTT GAGGATGAGC TTGCAGCAAG ACGGGACAAC 
TTGAAGCAGG TGATGGACGA GGGAGAGAGC ATGATCCAGA TCAAGCACTT GGGTTCCCCC 
AAAGTGCAGC AACGCATGAA TGATGTACAA AGACAGTGGC AACAACTGGA GGAACTGGCT 
GCATTCCGTA AACAGAATCT GCAAGACACC CAACGTTTCT TCCAGTTTCA GGGTGATGCT 
GATGACCTCA AGGCTTGGCT GGTCGATGCA ATGAGACAGA TGAGCAGTGA CGATGTTGGG 
CATGATGAGT ACACTACACA GCGACTGCTC AAGAAACACC GTGACTTAAG AGATGAGGCT 
GCTAAGAATG GAGCCACCAT TGATGCTCTA TCCAAACAGG CCAATGCACT TCCTGAAGAA 
CTACGGAATA CCCCTGACAT CCAGGGCCGT CTGAATGATA TACGGGATAT GTATATTGAG 
CTTCTGACCC TCTCTGATTT GAGGCAGAAG AAACTGGATG ACACCATGGC TCTTTACACT 
ATCTTCAGTG AGACAGATGC TTGTGAGCTC TGGATGGGCC AGAAAGAGAC CTGGCTGGTT 
GGGTTAGAGA CACCTGAGAA TTTGGAAGAT CTAGAAATTG TACAAAACAG GCTAAGCATT 
CTTGCTCAAG AAATGGGTAA TATGCAGACT CGAGTTGATA ACGTTAATAA AGCAGCCAAG 
CAACTTGAAG ACAGCAGACA TCCACAAACA AAACAAGTGA AAGACTGTCA AATTCGTCTC 
AATAAGAGAT GGGAGGCTTT TAAAGCAATG GTCGAAGATA AGAAACATAG GGTAGATTCT 
GCTCTTAGCC TACATAACTA TGATCTTGAT TGTGATGAGA CAGAATCATG GATAAAAGAA 
AAGACGAGAG TCATTGAGTC TACACAGGAT CTTGGGAATG ACCTTGCTGC TGTCATTACT 
ATTCAAAGGA AGCTTTTTGG CATGGAAAGA GACCTTGCTG CCATCCAAGA TAAGTTGGAT 
TCCCTGCGTG ACGAGGCCCA GAAGCTTGTA AAGGAGCACC CAGAAAATGC TTCTGATATA 
CTTGCCAGAC AGGAAGAGTT GGATGCAGCA TGGGACACCT TGAAACGTAC CCTGAAAGAC 
CGTGAAGACT CTCTTGGGGA GGTCAGCAAG CTGCAGACCT TCTTACAGGA CATGGACGAT 
TTTCAAGCTT GGTTGTTCAA GTCACAGAAG GCTGTTGCTT CTGAAGATAT GCCAGATGGT 
TTACCAGAGG CTGAGCAACT CCTGAATCTC CATGATGCTT TGAGGCATGA TATGGATGGC 
CATGAGGAAG ACTACCATCG TGTGAAAGAC ACAGGAGCTG CTGTTATTCA AGGTCAAGAA 
GATGATCCTC AGTACCAGCA ACTGGAACAG AGGCTGGAGG GTCTTGATAA GGGCTGGGGT 
GAGCTTCACA AAATGTGGGA CAGTCGCAAG AACTTCCTAG ATCAGGGTCT CGGTTTCCAA 
CAATTCATGA GAGATGCCAA ACAGGCTGAT GCCATCTTAA ATAATCAGGA ATACACTCTA 
GCCCACGTAG ACAAGCCTGA CACCCTTGAT GGAGCAGAGA AGGCCTTGAA GAAACATGAG 
GACTTTGTTA CCACCATGGA TGCTAATAAG GAGAAGATAC TTAGCACACT GGAGACTGGT 
CAGAGGCTGG TGGATAGTGA AAACCTTTAC TCTGGAAAAG TGAAAGACAA GATGAGTTCA 
ATCGAGGAAA GGAACAAAAA GAACCAAGAC AAAGCCAAGG AAGTCTCAGG GAAACTTAAA 
GACAACAGGG AACTTCAGCA CTTCCTTCAA AACACTCAGG ATCTTACATT GTGGATTAAT 
GAGAAAATGC TGACTGCACA AGATACATCA TATGATGAGG CTCGTAACCT TCATAGCAAG 
TGGCAAAAAC ACCAAGCTTT CATGGCAGAA CTAGCATCAA ACAAGGATTG GCTTCATAAC 
ATTGATAAGG AGGGACAGGA ACTGATGGAA TCCAAGCCTG AGTTTGAGCC TATTGTTAAG 
GATCGCCTTG CCAAGCTTCA TGAACTATGG GATAAGCTGG AGAGCACCAC ACAAGAAAAG 
GCTCGGCTAC TGTTTGATGC AAACCGTTCT GAGCTGTTCG ATCAGAGCCT AGCTGACCTA 
AAGAAATGGT TAGCTGAATT GCAGCAGCAA CTTCAGGGTG ATGTGGAAGA AGAAGTTAAG 
GATCTTACCA GTGCCAACAT CCTGCTAAAG AAACATCAGA TAACAGAGAA TCAGGTTCGG 
GACCGTGCAC GAGAGCTTGA AGAACTTCAG GAGGCTGTAG AGCAGCATGG TTCTCTTAGA 
GAGGACCAGC CTGAGCTTGA GATTGAGCAA CAAAATCTTC AGAGAGATTT CCAGAAGCTC 
CTCACACCTT TATCACAACG CAAGGGAAAG CTGGAGGCTG CTAAGGCAGT GCACCAGTTC 
TTTAGAGATC TTGCTGATGA AATACTCTGG GTTAATGAAA GGTTACCAAT GGCTATGTCA 
GATGATCATG GTAACAATCT TCAGACTGTC CAACTTTTGC TTAAGAAGAA CCAATCCTTA 
CAAAAAGAGA TTGATGGTCA TCAACCACGC ATTGATGAAG TGTTAGAGCG GGGTCGGCGA 
ATGGCAGCAG CAGCAGAGGG CAGTCCAGAG GAAGAACGCA TGTCTGAGGA GATGAAGAAG 
CTTCAGGTGG TGTGGGCACA GCTACAGGAA GAGATGGCAA AACGAAGAGA ACGGCTTTAT 
GGCTCTAATG AGGCCCAACA GTATTACAAT GATGCAGATG ATTCTGAGGC CTGGATAGGT 
GAACAAGAAC TCTACATGAT TGCTGATGAG ATGGCAAAGG ATGAACAGAG TGCTATGATC 
ATGCTGAAGC GGCACCTGGT TCTTAAGCAG ACAGTGGATG ACTATGCATA TTCCATCCAG 
CAATTGGCAG ACCGTGCCCA GAAGATGCTG GCTGAAGAGC ACCCAGATGG TGAGGCTATC 
ATCCGAAGAC AAGGACAAGT TGATAAGCAG TATGCTGGGC TGAAGGAGCT GGCAGAAGAC 
CGCAAAAAGA AGCTGGACCA CACATATCAC CATTTCCTGC TGAGTCGGGA AGTGGAGGAT 
CTAGAGCAGT GGATTGCAGA GCGAGATGTA GTGGCTTCAT CACAAGAAAT GGGCCAAGAC 
CTTGATCATG TGACGATCTT GCGAGACAAG TTCAGGGAGT TTGCACGGGA AACAGGGACA 
GTGGGTCAAG AACGTGTAGA TACTGTGAAC CGGATCATAG ATGAACTAAT TGAAGGAGGC 
CACAGTGAGT CAGCGACATT GGCCGAGTGG AAGGATGGAG TAAATGAGAG TTGGGCAGAT 
TTGCTGGAGC TCATTGACAC TCGTGCCCAG CTCCTCACAT CCTCTTATGA CCTCCTTAAG 
TATTTTTATG ATGGAAAAGA GCTGGTTGGT CACATTGAGG AAAAGAAGAA TGAACTTCCT 
GAAGACTTGG GAGAAGACTT CAGCAAAGCA GAATCATTCC ACCGAATGCA TGCTGCATTT 
GAGAGGGATA TCAGCTCCTT GGGCAAACAG GTAAAACAGT TCCAGGAGAC AGCAGCTAGA 
TTGCATGCCC AGTATGCAGG AGATCAGGCT ACTGCCATTC AAGCAACTGA AAAAGAGGTT 
GTTGAGGCCT GGAAGGGTCT GTTGGATGCT TGTGCGGGTA GGAGGAAACA GCTGGAGGAG 
ACAGCAGACA AATTCCGCTT TTTCACCATG GTGAGAGACC TAATGGCCTG GATGGAAAGC 
ATCCTTCAAC AGATAGAGAC ACAGGAAAAA CCAAGAGACG TCTCCTCAGT GGAGCTTCTG 
ATGAAGTATC ATCAAGGGAT CCGTGCTGAA ATTGAGACCC GTGGGCCAAA GTTTAATCAG 
TGTGTGCAAC TTGGACAGGC TCTCCTGGAA CGCAAGCACA AGGACTCTGC AGAGATTAAA 
GAGAAGCTGA TGCAACTGGT AGAGAAGAGA AAAGAGATGA TGCTCAAATG GGATGATCGA 
TGGGACTGGC TCAGACTTTT ACTGGAGGTA TGTCAGTTTG CTCGAGATGC TTCTGTGGCA 
GAGGCATGGT TGATTGCCCA GGAGCCATAT GTGGCCAGCA AGGACGTGGG TCAGACAGTC 
GATGAGGTTG AAAAACTGCT CAAAAGGCAT GAAGCATTTG AAAAATCTAC TGCAACATGG 
GAAGAGCGTT TCTCAGCATT GGAACGTCTT ACAACATTGG AGTTGTTGGA AATTCGGAAA 
CAACAACAAG AAATACAACA GTACAGACAA GAGGCAGAGA AAGAAAGCAG GAGAGAGGAC 
ACTGGTTTTG CAGAGGAGTC TTCACAACTT TACCCTACAG AAGAACAGTC TCTGTCTGGT 
TTAGGAGTAA TAGAGCCTAG TTCTGGTGGA GTTGATGGCA CAGCAGGGGA ATCCACTGTG 
CCTTTAATCT CTGAGATGCA GGAAAGTGTC TCACTAGAGC TGGACCCTTC CACATCCATT 
CAAGTTACCA AAGAGACAGA AAAAGCTGCC TCGCTTCCTA CAGAATCTAC ACAAGTTCAA 
CCAGTCCTGA TGGAGGGAAC TCTAGCCCGC AAGCATGAGT TGGAGGGACC TAACAAGAAG 
GCACCCAACA GGTCCTGGAA CAATCTGTAT TGTGTGCTTA AACCTGGACA CCTCTCTATC 
TACAAGGATG CCAAGAGCTT TAGTCACAGT GTAACCTTCC ATGGTGAGGA ACCCCTGACC 
CTCACCAATT CTTCATGCGA GATCTTGACC AACTATAAGA AAAAGAAGCA GGTGTTCAAA 
CTCCGACTTG GAGATGGAAG TGAATATCTG TTCCAGTGTA AAGACGAGGA GGAGCTTCAG 
AACTGGACTC AGGCTATTGA GCAGGCTGCA CAAGTTCAAA TAGAAGAACC AGTTGCAGGG 
CCATCAGGAG TGAAGGCACT AAGCCTACCT CCACCTTCCT CCACGACTCT AGAGGTTCCC 
TCAACGAAGA AGGACAAGGA AAAGAGGTTT AGTCTGTTTG CTAAGAAGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting hematopoietic and renal cell heterogeneity in adult zebrafish at single-cell resolution using RNA sequencing.
The Journal of experimental medicine214(10)2875-2887(2017 Oct)
Tang Q,Iyer S,Lobbardi R,Moore JC,Chen H,Lareau C,Hebert C,Shaw ML,Neftel C,Suva ML,Ceol CJ,Bernards A,Aryee M,Pinello L,Drummond IA,Langenau DM

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)
Smith LC,Clark JC,Bisesi JH Jr,Ferguson PL,Sabo-Attwood T

Single-cell transcriptional analysis of normal, aberrant, and malignant hematopoiesis in zebrafish.
The Journal of experimental medicine213(6)979-92(2016 May)
Moore FE,Garcia EG,Lobbardi R,Jain E,Tang Q,Moore JC,Cortes M,Molodtsov A,Kasheta M,Luo CC,Garcia AJ,Mylvaganam R,Yoder JA,Blackburn JS,Sadreyev RI,Ceol CJ,North TE,Langenau DM

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SPTB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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