SPTBN1 cDNA ORF clone, Homo sapiens(human)

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18923 NM_003128.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu30267 NM_178313.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu18923 XM_005264517.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu18923 XM_017004781.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu18923 XM_006712087.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu18923 XM_017004779.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu18923 XM_017004780.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu35574 XM_005264518.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OHu35574 XM_005264518.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu18923 XM_006712087.2 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu18923 NM_003128.2 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version NM_003128.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP218428.1, BP312037.1, AB209748.1, AC093110.4 and BM679686.1. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2006 this sequence version replaced NM_003128.1. Transcript Variant: This variant (1), also known as long or sigma-1, represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794662.1, AB371586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003119.2
    CDS250..7344
    Translation

    Target ORF information:

    RefSeq Version NM_003128.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003128.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version NM_003128.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-16
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP218428.1, BP312037.1, AB209748.1, AC093110.4 and BM679686.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003128.2. Transcript Variant: This variant (1), also known as long or sigma-1, represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794662.1, AB371586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356805.9/ ENSP00000349259.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003119.2
    CDS240..7334
    Translation

    Target ORF information:

    RefSeq Version NM_003128.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003128.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu30267
    Clone ID Related Accession (Same CDS sequence) NM_178313.2
    Accession Version NM_178313.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF327441.1, CR980403.1, AC093110.4 and AK025071.1. On Aug 16, 2006 this sequence version replaced NM_178313.1. Transcript Variant: This variant (2), also known as short or sigma-2, differs in the 5' UTR, 3' UTR, and coding region (compared to variant 1). The resulting protein (isoform 2) maintains the reading frame but has shorter and distinct sequences at both the N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF327441.1, BC137282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA
    GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC
    CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC
    CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC
    GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC
    CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA
    CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG
    AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC
    CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA
    CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG
    TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT
    CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC
    TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG
    AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT
    GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC
    CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC
    GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG
    GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT
    GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC
    TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC
    CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC
    GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG
    TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT
    CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC
    TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC
    AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG
    AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC
    CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC
    AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG
    AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC
    GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC
    TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA
    ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT
    GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG
    TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG
    TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG
    CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG
    ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG
    TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG
    CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG
    ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG
    CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC
    TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG
    AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG
    CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC
    TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA
    GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC
    TTTAATAGCA GACGGACAGC CAGTGACCAG CCATGGTCTG GACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_842565.2
    CDS386..6853
    Translation

    Target ORF information:

    RefSeq Version NM_178313.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_178313.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA
    GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC
    CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC
    CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC
    GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC
    CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA
    CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG
    AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC
    CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA
    CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG
    TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT
    CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC
    TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG
    AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT
    GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC
    CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC
    GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG
    GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT
    GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC
    TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC
    CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC
    GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG
    TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT
    CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC
    TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC
    AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG
    AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC
    CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC
    AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG
    AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC
    GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC
    TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA
    ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT
    GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG
    TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG
    TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG
    CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG
    ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG
    TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG
    CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG
    ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG
    CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC
    TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG
    AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG
    CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC
    TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA
    GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC
    TTTAATAGCA GACGGACAGC CAGTGACCAG CCATGGTCTG GACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version XM_006712087.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712150.1
    CDS239..7333
    Translation

    Target ORF information:

    RefSeq Version XM_006712087.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712087.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version XM_017004780.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860269.1
    CDS220..7314
    Translation

    Target ORF information:

    RefSeq Version XM_017004780.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version XM_006712087.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712150.1
    CDS123..7217
    Translation

    Target ORF information:

    RefSeq Version XM_006712087.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712087.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version XM_005264517.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264574.1
    CDS159..7253
    Translation

    Target ORF information:

    RefSeq Version XM_005264517.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264517.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version XM_017004779.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860268.1
    CDS266..7360
    Translation

    Target ORF information:

    RefSeq Version XM_017004779.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004779.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu18923
    Clone ID Related Accession (Same CDS sequence) XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
    Accession Version XM_017004781.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860270.1
    CDS221..7315
    Translation

    Target ORF information:

    RefSeq Version XM_017004781.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG
    GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT
    CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA
    ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG
    CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC
    ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT
    GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG
    AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT
    TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA
    GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT
    TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG
    CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG
    TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT
    GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG
    CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC
    AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG
    ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG
    CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA
    GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC
    TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC
    AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT
    CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC
    CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC
    AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA
    AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC
    ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT
    GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG
    ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC
    GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT
    GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC
    GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC
    GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA
    TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC
    GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC
    CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC
    CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG
    GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT
    ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG
    AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC
    GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT
    GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT
    GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA
    AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA
    GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT
    CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA
    TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT
    ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC
    TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC
    CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG
    GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT
    GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG
    GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC
    AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT
    GGCAAAAAGA AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35574
    Clone ID Related Accession (Same CDS sequence) XM_005264518.1 , XM_005264518.2
    Accession Version XM_005264518.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA
    GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC
    CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC
    CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC
    GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC
    CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA
    CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG
    AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC
    CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA
    CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG
    TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT
    CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC
    TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG
    AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT
    GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC
    CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC
    GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG
    GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT
    GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC
    TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC
    CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC
    GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG
    TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT
    CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC
    TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC
    AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG
    AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC
    CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC
    AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG
    AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC
    GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC
    TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA
    ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT
    GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG
    TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG
    TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG
    CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG
    ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG
    TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG
    CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG
    ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG
    CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC
    TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG
    AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG
    CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC
    TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC
    GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT
    CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG
    GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC
    AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC
    GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG
    GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA
    GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264575.1
    CDS1044..8099
    Translation

    Target ORF information:

    RefSeq Version XM_005264518.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264518.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA
    GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC
    CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC
    CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC
    GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC
    CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA
    CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG
    AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC
    CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA
    CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG
    TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT
    CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC
    TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG
    AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT
    GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC
    CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC
    GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG
    GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT
    GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC
    TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC
    CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC
    GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG
    TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT
    CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC
    TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC
    AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG
    AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC
    CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC
    AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG
    AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC
    GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC
    TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA
    ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT
    GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG
    TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG
    TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG
    CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG
    ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG
    TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG
    CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG
    ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG
    CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC
    TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG
    AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG
    CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC
    TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC
    GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT
    CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG
    GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC
    AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC
    GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG
    GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA
    GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35574
    Clone ID Related Accession (Same CDS sequence) XM_005264518.1 , XM_005264518.2
    Accession Version XM_005264518.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA
    GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC
    CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC
    CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC
    GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC
    CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA
    CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG
    AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC
    CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA
    CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG
    TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT
    CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC
    TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG
    AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT
    GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC
    CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC
    GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG
    GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT
    GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC
    TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC
    CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC
    GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG
    TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT
    CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC
    TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC
    AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG
    AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC
    CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC
    AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG
    AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC
    GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC
    TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA
    ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT
    GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG
    TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG
    TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG
    CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG
    ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG
    TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG
    CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG
    ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG
    CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC
    TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG
    AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG
    CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC
    TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC
    GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT
    CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG
    GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC
    AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC
    GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG
    GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA
    GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264575.1
    CDS1057..8112
    Translation

    Target ORF information:

    RefSeq Version XM_005264518.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA
    GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC
    CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC
    CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC
    GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC
    CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA
    CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG
    AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA
    TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC
    CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA
    CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG
    TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT
    CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC
    TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG
    AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT
    GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC
    CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC
    GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG
    GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT
    GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC
    TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC
    CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC
    GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG
    TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT
    CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC
    TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC
    AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG
    AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC
    CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC
    AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG
    AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC
    GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC
    TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA
    ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT
    GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG
    TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG
    TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG
    CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG
    ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG
    TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG
    CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG
    ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG
    CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC
    TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG
    AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT
    CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG
    CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG
    CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC
    TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC
    GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT
    CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG
    GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC
    AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC
    GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG
    GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA
    GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.