SPTBN1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006712087.3	XP_006712150.1	spectrin beta chain, non-erythrocytic 1 isoform X1
NM_178313.2	NP_842565.2	spectrin beta chain, non-erythrocytic 1 isoform 2
XM_017004780.1	XP_016860269.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_005264518.1	XP_005264575.1	spectrin beta chain, non-erythrocytic 1 isoform X2
XM_005264518.2	XP_005264575.1	spectrin beta chain, non-erythrocytic 1 isoform X2
XM_006712087.2	XP_006712150.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_005264517.2	XP_005264574.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_017004779.1	XP_016860268.1	spectrin beta chain, non-erythrocytic 1 isoform X1
NM_003128.2	NP_003119.2	spectrin beta chain, non-erythrocytic 1 isoform 1
NM_003128.3	NP_003119.2	spectrin beta chain, non-erythrocytic 1 isoform 1
XM_017004781.1	XP_016860270.1	spectrin beta chain, non-erythrocytic 1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18923	NM_003128.3 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu30267	NM_178313.2 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu18923	XM_005264517.2 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu18923	XM_017004781.1 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu18923	XM_006712087.3 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu18923	XM_017004779.1 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu18923	XM_017004780.1 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu35574	XM_005264518.2 Latest version!	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
View Old Accession Versions >>
OHu35574	XM_005264518.1	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu18923	XM_006712087.2	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu18923	NM_003128.2	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

SPTBN1 ( NM_003128.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003119.2 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	NM_003128.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP218428.1, BP312037.1, AB209748.1, AC093110.4 and BM679686.1. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2006 this sequence version replaced NM_003128.1. Transcript Variant: This variant (1), also known as long or sigma-1, represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794662.1, AB371586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003119.2
CDS	250..7344
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	NM_003128.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003128.2

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( NM_003128.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003119.2 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	NM_003128.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP218428.1, BP312037.1, AB209748.1, AC093110.4 and BM679686.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003128.2. Transcript Variant: This variant (1), also known as long or sigma-1, represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794662.1, AB371586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000356805.9/ ENSP00000349259.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003119.2
CDS	240..7334
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	NM_003128.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003128.3

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( NM_178313.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_842565.2 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 2, mRNA.

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CloneID	OHu30267
Clone ID Related Accession (Same CDS sequence)	NM_178313.2
Accession Version	NM_178313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF327441.1, CR980403.1, AC093110.4 and AK025071.1. On Aug 16, 2006 this sequence version replaced NM_178313.1. Transcript Variant: This variant (2), also known as short or sigma-2, differs in the 5' UTR, 3' UTR, and coding region (compared to variant 1). The resulting protein (isoform 2) maintains the reading frame but has shorter and distinct sequences at both the N- and C-termini, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF327441.1, BC137282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC 
CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC 
TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC 
CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC 
GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC 
CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT 
GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG 
CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC 
GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC 
TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC 
GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA 
GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCATGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_842565.2
CDS	386..6853
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDQPWSGL

Target ORF information:

RefSeq Version	NM_178313.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178313.2

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC 
CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC 
TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC 
CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC 
GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC 
CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT 
GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG 
CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC 
GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC 
TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC 
GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA 
GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC 
TTTAATAGCA GACGGACAGC CAGTGACCAG CCATGGTCTG GACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_006712087.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712150.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	XM_006712087.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712087.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712150.1
CDS	239..7333
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_006712087.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712087.3

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_017004780.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860269.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	XM_017004780.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860269.1
CDS	220..7314
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_017004780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004780.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_006712087.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712150.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	XM_006712087.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712150.1
CDS	123..7217
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_006712087.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712087.2

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_005264517.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264574.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	XM_005264517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264574.1
CDS	159..7253
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_005264517.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264517.2

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_017004779.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860268.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	XM_017004779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860268.1
CDS	266..7360
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_017004779.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004779.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_017004781.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860270.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.

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CloneID	OHu18923
Clone ID Related Accession (Same CDS sequence)	XM_006712087.3 , XM_017004780.1 , XM_006712087.2 , XM_005264517.2 , XM_017004779.1 , NM_003128.2 , NM_003128.3 , XM_017004781.1
Accession Version	XM_017004781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860270.1
CDS	221..7315
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_017004781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004781.1

ORF Insert Sequence:

ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
GTCAACAACC GCTGGGATGT CGACGACTGG GACAATGAGA ACAGCTCTGC GCGGCTTTTT 
GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAAG CCGTGCAGAA GAAGACCTTC 
ACCAAGTGGG TCAATTCCCA CCTTGCCCGT GTGTCCTGCC GGATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGCTTAG AGAATGTGGA CAAGGCCCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCATGA CATCGTGGAT 
GGAAACCACC GGCTGACCCT TGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG 
TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGACA AACTAAAGAA ATCTAACGCA CACTACAACC TGCAGAATGC ATTTAATCTG 
GCAGAACAGC ACCTCGGCCT CACTAAACTG TTGGACCCCG AAGACATCAG CGTGGACCAT 
CCTGATGAGA AGTCCATAAT CACTTATGTG GTGACTTATT ACCACTACTT CTCTAAGATG 
AAGGCCTTAG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA 
GAAAAAATGA TTGAAAAGTA TGAATCACTT GCCTCTGACC TTCTGGAATG GATTGAACAA 
ACCATCATCA TTCTGAACAA TCGCAAATTT GCCAATTCAC TGGTCGGGGT TCAACAGCAG 
CTTCAGGCAT TCAACACTTA CCGCACTGTG GAGAAACCAC CCAAATTTAC TGAGAAGGGG 
AACTTGGAAG TGCTGCTCTT CACCATTCAG AGCAAGATGA GGGCCAACAA CCAGAAGGTC 
TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCGGAAC ACGAAAGAGA ACTGGCTTTG CGGAATGAGC TCATAAGACA GGAGAAACTG 
GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACTTG GCTGAGCGAA 
AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCCGCC 
ACAAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCAT ACGAGGAGCG TGTGCAGGCT 
GTGGTAGCCG TGGCCAGGGA GCTCGAGGCC GAGAATTACC ACGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACCTAC TGGAACTGCT CAGGGCCCGG 
AGACAGCGGC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA GTATTGTCTC AAGACTATGG CAAACACTTA 
CTTGGTGTGG AAGACCTGTT ACAGAAGCAC ACCCTGGTTG AAGCAGACAT TGGCATCCAG 
GCAGAGCGGG TGAGAGGTGT CAATGCCTCC GCCCAGAAGT TCGCAACAGA CGGGGAAGGT 
TACAAGCCCT GTGACCCCCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT 
CAAGAGCTTT GCCAGCTGGC GGCTGAGCGC AGGGCCCGTC TGGAAGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCGG ACGATTACGG GAAAGACCTG ACCAGCGTCA TGCGCCTGCT CAGCAAGCAC 
CGGGCGTTCG AGGACGAGAT GAGCGGCCGC AGTGGCCACT TTGAGCAGGC CATCAAGGAA 
GGCGAAGACA TGATCGCGGA GGAGCACTTC GGGTCGGAGA AGATCCGTGA GAGGATCATT 
TACATCCGGG AGCAGTGGGC CAACCTAGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG 
GAGGAGGCCT CCCTGCTGCA CCAGTTCCAG GCAGATGCTG ATGACATTGA TGCCTGGATG 
CTGGACATCC TCAAGATTGT CTCCAGCAGC GACGTGGGCC ACGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAACACAA GGACGTGGCG GAAGAGATCG CCAATTACAG GCCCACCCTT 
GACACGCTGC ACGAACAAGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCCAGACGTG 
AGGGGCAGGC TGTCGGGCAT CGAGGAGCGG TATAAGGAGG TGGCAGAGCT GACGCGGCTG 
CGGAAGCAGG CACTCCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGATC TGGAGGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCAGT GGTGAACCAG ATTGCACGCC AGCTGATGCA CAGCGGCCAC 
CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACAAGGTG GAGCCAGTTC 
AGAGAACTGG TTGACAGGAA GAAGGATGCC CTCCTGTCTG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATTCGGGAAA AGACCAAGGT CATCGAGTCC 
ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAGCGGG ACTTGGTGGC CATTGAGGCA AAGCTGAGTG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCCATCC TGTCTCGGCT GGCCGAGATC 
AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GAGAGGCCTC CCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTACGGGAC TTGGACGACT TCCAGTCCTG GCTCTCTAGG 
ACCCAGACAG CGATCGCCTC GGAGGACATG CCAAACACCC TGACCGAGGC TGAGAAGCTG 
CTCACGCAGC ACGAGAACAT CAAGAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACCG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGC TGCAGGCCCT GGACACTGGA TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGACAAAATC TCCTATCCCA GTCACATGCC TACCAGCAGT TCCTCAGAGA CACGAAGCAA 
GCCGAAGCCT TTCTTAACAA CCAGGAGTAT GTTCTGGCTC ACACTGAAAT GCCTACCACC 
TTGGAAGGAG CTGAAGCAGC AATTAAAAAG CAAGAGGACT TCATGACCAC CATGGACGCC 
AATGAGGAGA AGATCAATGC TGTGGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAC 
ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGACA TAGGAAGAAT 
CGTGAGACAG CCAGTGAACT TTTGATGAGG TTGAAGGACA ACAGGGATCT ACAGAAATTC 
CTGCAAGATT GTCAAGAGCT GTCTCTCTGG ATCAATGAGA AGATGCTCAC AGCCCAGGAC 
ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAAGG AATGCAGCTC 
ATTTCAGAAA AGCCTGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACTACCCAG ACAAAGGCCC AGCGGCTCTT TGATGCAAAC 
AAGGCCGAAC TTTTCACCCA GAGCTGTGCA GATCTAGACA AATGGCTGCA CGGCCTGGAG 
AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTGACCA GTGTCAATAT CCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGCGGAAGA AGGAGATCGA AGAGCTCCAA 
AGCCAAGCCC AGGCCCTGAG TCAGGAAGGG AAGAGCACCG ACGAGGTAGA CAGCAAGCGC 
CTCACCGTGC AGACCAAGTT CATGGAGTTG CTGGAGCCCT TGAACGAGAG GAAGCATAAC 
CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATCTTGTGG 
GTTGGAGAGA GGATGCCTTT GGCAACTTCC ACGGATCATG GCCACAACCT CCAGACTGTG 
CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAAGAAA TCCAGGGGCA CCAGCCTCGC 
ATTGACGACA TCTTTGAGAG GAGCCAAAAC ATCGTCACTG ACAGCAGCAG CCTCAGCGCT 
GAGGCCATCA GACAGAGGCT TGCCGACCTG AAGCAGCTGT GGGGTCTCCT CATTGAGGAG 
ACAGAGAAAC GCCACAGGCG GCTGGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTTGAC 
GCTGCTGAGG CCGAAGCCTG GATGAGCGAG CAGGAGCTGT ACATGATGTC AGAGGAGAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAGAAGC ACCAGATCTT AGAACAAGCT 
GTGGAGGACT ATGCAGAGAC CGTGCATCAG CTCTCCAAGA CCAGCCGGGC CCTGGTGGCC 
GACAGCCATC CTGAAAGTGA GCGCATTAGC ATGCGGCAGT CCAAAGTGGA TAAACTGTAC 
GCTGGTCTGA AAGACCTTGC TGAAGAGAGA AGAGGCAAGC TGGATGAGAG ACACAGGTTA 
TTCCAGCTCA ACCGGGAGGT GGACGACCTG GAGCAGTGGA TCGCTGAGAG GGAGGTGGTC 
GCAGGGTCCC ATGAACTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGATTC 
CGGGAGTTTG CCCGAGACAC CGGGAACATT GGGCAGGAGC GCGTGGACAC GGTCAATCAC 
CTGGCAGATG AGCTCATCAA CTCTGGACAT TCAGATGCCG CCACCATCGC TGAATGGAAG 
GATGGCCTCA ATGAAGCCTG GGCCGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT 
CTTGCCGCTT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGT 
ATACAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG 
ACCTTACAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACACAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAGGCGGCCT ATGCGGGTGA CAAGGCCGAC 
GATATCCAGA AGCGCGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTCCT GGACGCCTGT 
GAGAGCCGCA GGGTGCGGCT GGTGGACACA GGGGACAAGT TCCGCTTCTT CAGCATGGTG 
CGCGACCTCA TGCTCTGGAT GGAGGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAGCCA 
AGGGATGTAT CATCTGTTGA ACTCTTAATG AATAATCATC AAGGCATCAA AGCTGAAATT 
GATGCACGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GTTGGCGAGA 
AAACACTATG CATCTGAGGA GATCAAGGAA AAATTACTGC AGTTGACGGA AAAGAGGAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTCT GGAGGTCCAT 
CAGTTCTCAA GAGACGCCAG TGTGGCCGAG GCCTGGCTGC TTGGACAGGA GCCGTACCTA 
TCCAGCCGAG AGATAGGCCA GAGCGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG 
GCATTTGAAA AGTCTGCAGC AACCTGGGAT GAGAGGTTCT CTGCCCTGGA AAGGCTGACT 
ACATTGGAGT TACTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG 
CCTTCTCCCG AGCCGAGCAC GAAGGTTTCA GAGGAAGCCG AGTCCCAGCA GCAGTGGGAT 
ACTTCAAAAG GAGAACAAGT TTCCCAAAAC GGTTTGCCAG CTGAACAGGG ATCTCCACGG 
ATGGCAGAAA CGGTGGACAC AAGCGAAATG GTCAACGGCG CTACAGAACA AAGGACGAGC 
TCTAAAGAGT CCAGCCCCAT CCCCTCCCCG ACCTCTGATC GTAAAGCCAA GACTGCCCTC 
CCAGCCCAGA GTGCCGCCAC CTTACCAGCC AGAACCCAGG AGACACCTTC GGCCCAGATG 
GAAGGCTTCC TCAATCGGAA ACACGAGTGG GAGGCCCACA ATAAGAAAGC CTCAAGCAGG 
TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA 
AAGACTGCTG CTTCTGGAAT TCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCT 
GTCTGCGAAG TGGCCCTTGA TTACAAAAAG AAGAAACACG TATTCAAGCT AAGACTAAAT 
GATGGCAATG AGTACCTCTT CCAAGCCAAA GACGATGAGG AAATGAACAC ATGGATCCAG 
GCTATCTCTT CCGCCATCTC CTCTGATAAA CACGAGGTGT CTGCCAGCAC CCAGAGCACG 
CCAGCATCCA GCCGCGCGCA GACCCTCCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCC 
AGTCCCGGCA AGCGGGAAAA GGACAAAGAG AAAGACAAAG AGAAGCGGTT CAGCCTTTTT 
GGCAAAAAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_005264518.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264575.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

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CloneID	OHu35574
Clone ID Related Accession (Same CDS sequence)	XM_005264518.1 , XM_005264518.2
Accession Version	XM_005264518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC 
CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC 
TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC 
CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC 
GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC 
CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT 
GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG 
CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC 
GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC 
TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC 
GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA 
GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT 
CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG 
GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC 
GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG 
GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA 
GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264575.1
CDS	1044..8099
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_005264518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264518.1

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC 
CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC 
TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC 
CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC 
GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC 
CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT 
GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG 
CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC 
GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC 
TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC 
GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA 
GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT 
CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG 
GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC 
GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG 
GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA 
GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_005264518.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264575.1 Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

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CloneID	OHu35574
Clone ID Related Accession (Same CDS sequence)	XM_005264518.1 , XM_005264518.2
Accession Version	XM_005264518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC 
CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC 
TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC 
CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC 
GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC 
CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT 
GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG 
CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC 
GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC 
TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC 
GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA 
GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT 
CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG 
GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC 
GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG 
GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA 
GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264575.1
CDS	1057..8112
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLVLSQDYGKHLLGVEDLLQKHTLVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSSDVGHDEYSTQSLVKKHKDVAEEIANYRPTLDTLHEQASALPQEHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNRETASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHLADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACESRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKTAASGIPYHSEVPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMNTWIQAISSAISSDKHEVSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_005264518.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264518.2

ORF Insert Sequence:

ATGGAATTGC AGAGGACGTC TAGTATCTCC GGGCCGCTGT CGCCGGCGTA CACGGGGCAG 
GTGCCTTACA ACTACAACCA GCTGGAAGGC AGATTCAAGC AGCTGCAAGA TGAGCGTGAA 
GCCGTGCAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG TGTGTCCTGC 
CGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGCTTA 
GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG 
GGGTCCCATG ACATCGTGGA TGGAAACCAC CGGCTGACCC TTGGCCTCAT CTGGACCATC 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAAACTG AAGACAACAA AGAGAAGAAA 
TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCTAACGC ACACTACAAC 
CTGCAGAATG CATTTAATCT GGCAGAACAG CACCTCGGCC TCACTAAACT GTTGGACCCC 
GAAGACATCA GCGTGGACCA TCCTGATGAG AAGTCCATAA TCACTTATGT GGTGACTTAT 
TACCACTACT TCTCTAAGAT GAAGGCCTTA GCTGTTGAAG GAAAACGAAT TGGAAAGGTG 
CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAATCACT TGCCTCTGAC 
CTTCTGGAAT GGATTGAACA AACCATCATC ATTCTGAACA ATCGCAAATT TGCCAATTCA 
CTGGTCGGGG TTCAACAGCA GCTTCAGGCA TTCAACACTT ACCGCACTGT GGAGAAACCA 
CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTCT TCACCATTCA GAGCAAGATG 
AGGGCCAACA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCGGAA CACGAAAGAG AACTGGCTTT GCGGAATGAG 
CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG 
AGGGAGACTT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC 
CTTCCTGCAG TTGAGGCCGC CACAAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCA 
TACGAGGAGC GTGTGCAGGC TGTGGTAGCC GTGGCCAGGG AGCTCGAGGC CGAGAATTAC 
CACGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACCTA 
CTGGAACTGC TCAGGGCCCG GAGACAGCGG CTCGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAAA TGAAGGTGCT AGTATTGTCT 
CAAGACTATG GCAAACACTT ACTTGGTGTG GAAGACCTGT TACAGAAGCA CACCCTGGTT 
GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCCTC CGCCCAGAAG 
TTCGCAACAG ACGGGGAAGG TTACAAGCCC TGTGACCCCC AGGTGATCCG AGACCGCGTG 
GCCCACATGG AGTTCTGTTA TCAAGAGCTT TGCCAGCTGG CGGCTGAGCG CAGGGCCCGT 
CTGGAAGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCG GACGATTACG GGAAAGACCT GACCAGCGTC 
ATGCGCCTGC TCAGCAAGCA CCGGGCGTTC GAGGACGAGA TGAGCGGCCG CAGTGGCCAC 
TTTGAGCAGG CCATCAAGGA AGGCGAAGAC ATGATCGCGG AGGAGCACTT CGGGTCGGAG 
AAGATCCGTG AGAGGATCAT TTACATCCGG GAGCAGTGGG CCAACCTAGA GCAGCTCTCG 
GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCCCTGCTGC ACCAGTTCCA GGCAGATGCT 
GATGACATTG ATGCCTGGAT GCTGGACATC CTCAAGATTG TCTCCAGCAG CGACGTGGGC 
CACGATGAGT ATTCCACACA GTCTCTGGTC AAGAAACACA AGGACGTGGC GGAAGAGATC 
GCCAATTACA GGCCCACCCT TGACACGCTG CACGAACAAG CCAGCGCCCT CCCCCAGGAG 
CATGCCGAGT CTCCAGACGT GAGGGGCAGG CTGTCGGGCA TCGAGGAGCG GTATAAGGAG 
GTGGCAGAGC TGACGCGGCT GCGGAAGCAG GCACTCCAGG ACACTCTGGC CCTGTACAAG 
ATGTTCAGCG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAT CTGGAGGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCAG TGGTGAACCA GATTGCACGC 
CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC 
AACACAAGGT GGAGCCAGTT CAGAGAACTG GTTGACAGGA AGAAGGATGC CCTCCTGTCT 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATTCGGGAA 
AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACTTGGTGG CCATTGAGGC AAAGCTGAGT 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCCATC 
CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC 
CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTACGGGA CTTGGACGAC 
TTCCAGTCCT GGCTCTCTAG GACCCAGACA GCGATCGCCT CGGAGGACAT GCCAAACACC 
CTGACCGAGG CTGAGAAGCT GCTCACGCAG CACGAGAACA TCAAGAACGA GATCGACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACC 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG ATGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGACAAAAT CTCCTATCCC AGTCACATGC CTACCAGCAG 
TTCCTCAGAG ACACGAAGCA AGCCGAAGCC TTTCTTAACA ACCAGGAGTA TGTTCTGGCT 
CACACTGAAA TGCCTACCAC CTTGGAAGGA GCTGAAGCAG CAATTAAAAA GCAAGAGGAC 
TTCATGACCA CCATGGACGC CAATGAGGAG AAGATCAATG CTGTGGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGAC ATAGGAAGAA TCGTGAGACA GCCAGTGAAC TTTTGATGAG GTTGAAGGAC 
AACAGGGATC TACAGAAATT CCTGCAAGAT TGTCAAGAGC TGTCTCTCTG GATCAATGAG 
AAGATGCTCA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG 
TTGAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATC 
GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCTGAGA CGGAAGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACTACCCA GACAAAGGCC 
CAGCGGCTCT TTGATGCAAA CAAGGCCGAA CTTTTCACCC AGAGCTGTGC AGATCTAGAC 
AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTGACC 
AGTGTCAATA TCCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGCGGAAG 
AAGGAGATCG AAGAGCTCCA AAGCCAAGCC CAGGCCCTGA GTCAGGAAGG GAAGAGCACC 
GACGAGGTAG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGTT GCTGGAGCCC 
TTGAACGAGA GGAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT 
GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCAACTTC CACGGATCAT 
GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAAGAA 
ATCCAGGGGC ACCAGCCTCG CATTGACGAC ATCTTTGAGA GGAGCCAAAA CATCGTCACT 
GACAGCAGCA GCCTCAGCGC TGAGGCCATC AGACAGAGGC TTGCCGACCT GAAGCAGCTG 
TGGGGTCTCC TCATTGAGGA GACAGAGAAA CGCCACAGGC GGCTGGAGGA GGCGCACAGG 
GCCCAGCAGT ACTACTTTGA CGCTGCTGAG GCCGAAGCCT GGATGAGCGA GCAGGAGCTG 
TACATGATGT CAGAGGAGAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAGAAG 
CACCAGATCT TAGAACAAGC TGTGGAGGAC TATGCAGAGA CCGTGCATCA GCTCTCCAAG 
ACCAGCCGGG CCCTGGTGGC CGACAGCCAT CCTGAAAGTG AGCGCATTAG CATGCGGCAG 
TCCAAAGTGG ATAAACTGTA CGCTGGTCTG AAAGACCTTG CTGAAGAGAG AAGAGGCAAG 
CTGGATGAGA GACACAGGTT ATTCCAGCTC AACCGGGAGG TGGACGACCT GGAGCAGTGG 
ATCGCTGAGA GGGAGGTGGT CGCAGGGTCC CATGAACTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGATT CCGGGAGTTT GCCCGAGACA CCGGGAACAT TGGGCAGGAG 
CGCGTGGACA CGGTCAATCA CCTGGCAGAT GAGCTCATCA ACTCTGGACA TTCAGATGCC 
GCCACCATCG CTGAATGGAA GGATGGCCTC AATGAAGCCT GGGCCGACCT CCTGGAGCTC 
ATTGACACAA GAACACAGAT TCTTGCCGCT TCCTATGAAC TGCACAAGTT TTACCACGAT 
GCCAAGGAGA TCTTTGGGCG TATACAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGG 
AGAGATCAGA ACACAGTGGA GACCTTACAG AGAATGCACA CTACATTTGA GCATGACATC 
CAGGCTCTGG GCACACAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAGGCGGCC 
TATGCGGGTG ACAAGGCCGA CGATATCCAG AAGCGCGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTCC TGGACGCCTG TGAGAGCCGC AGGGTGCGGC TGGTGGACAC AGGGGACAAG 
TTCCGCTTCT TCAGCATGGT GCGCGACCTC ATGCTCTGGA TGGAGGATGT CATCCGGCAG 
ATCGAGGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTCTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT TGATGCACGT AATGACAGTT TCACAACCTG CATTGAACTT 
GGGAAATCCC TGTTGGCGAG AAAACACTAT GCATCTGAGG AGATCAAGGA AAAATTACTG 
CAGTTGACGG AAAAGAGGAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAATGGTTA 
AGACTGATTC TGGAGGTCCA TCAGTTCTCA AGAGACGCCA GTGTGGCCGA GGCCTGGCTG 
CTTGGACAGG AGCCGTACCT ATCCAGCCGA GAGATAGGCC AGAGCGTGGA CGAGGTGGAG 
AAGCTCATCA AGCGCCACGA GGCATTTGAA AAGTCTGCAG CAACCTGGGA TGAGAGGTTC 
TCTGCCCTGG AAAGGCTGAC TACATTGGAG TTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAGGAGAGGA AGAGGCGGCC GCCTTCTCCC GAGCCGAGCA CGAAGGTTTC AGAGGAAGCC 
GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAACAAG TTTCCCAAAA CGGTTTGCCA 
GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAAGCGAAAT GGTCAACGGC 
GCTACAGAAC AAAGGACGAG CTCTAAAGAG TCCAGCCCCA TCCCCTCCCC GACCTCTGAT 
CGTAAAGCCA AGACTGCCCT CCCAGCCCAG AGTGCCGCCA CCTTACCAGC CAGAACCCAG 
GAGACACCTT CGGCCCAGAT GGAAGGCTTC CTCAATCGGA AACACGAGTG GGAGGCCCAC 
AATAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA 
ATGGGTTTCT ACAAAGATGC AAAGACTGCT GCTTCTGGAA TTCCCTACCA CAGCGAGGTC 
CCTGTGAGTT TGAAAGAAGC TGTCTGCGAA GTGGCCCTTG ATTACAAAAA GAAGAAACAC 
GTATTCAAGC TAAGACTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGACGATGAG 
GAAATGAACA CATGGATCCA GGCTATCTCT TCCGCCATCT CCTCTGATAA ACACGAGGTG 
TCTGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGCGCGC AGACCCTCCC CACCAGCGTC 
GTCACCATCA CCAGCGAGTC CAGTCCCGGC AAGCGGGAAA AGGACAAAGA GAAAGACAAA 
GAGAAGCGGT TCAGCCTTTT TGGCAAAAAG AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPTBN1
Entrez Gene ID	6711
Full Name	spectrin beta, non-erythrocytic 1
Synonyms	ELF,HEL102,SPTB2,betaSpII
General protein information	Preferred Names spectrin beta, non-erythrocytic 1 Names spectrin beta chain, non-erythrocytic 1 beta-G spectrin beta-II spectrin beta-fodrin beta-spectrin 2 beta-spectrin II beta-spectrin non-erythrocytic 1 embryonic liver beta-fodrin epididymis luminal protein 102 fodrin beta chain spectrin beta chain, brain 1 spectrin, non-erythroid beta chain 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p16.2
Summary	Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	182790

Gallus gallus (chicken)	SPTBN1	NP_001186354.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010895	XP_309796.2
Caenorhabditis elegans (roundworm)	unc-70	NP_001024053.1
Canis lupus familiaris (dog)	SPTBN1	XP_531827.2
Pan troglodytes (chimpanzee)	SPTBN1	XP_001154155.1
	SPTBN1	XP_001114804.1
Bos taurus (cattle)	SPTBN1	NP_001179205.1
Drosophila melanogaster (fruit fly)	beta-Spec	NP_001259660.1
Homo sapiens (human)	SPTBN1	NP_003119.2
Mus musculus (house mouse)	Sptbn1	NP_787030.2
Rattus norvegicus (Norway rat)	Sptbn1	NP_001013148.1
Danio rerio (zebrafish)	sptb	NP_571600.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPTBN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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