Sptbn1 cDNA ORF clone, Mus musculus(house mouse)

The following Sptbn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptbn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu22354 XM_030245736.1
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu22354 XM_006514600.4
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu22354 XM_030245738.1
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu22354 XM_006514599.4
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu22354 XM_030245737.1
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu45140 XM_006514601.3
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OMu22257 NM_009260.2
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1581.30
$2259.00
OMu22354 NM_175836.2
Latest version!
Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OMu45140 XM_006514601.2 Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu22354 XM_006514599.3 Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OMu22354 XM_006514600.3 Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_006514599.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514599.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006514662.1
    CDS484..7575
    Translation

    Target ORF information:

    RefSeq Version XM_006514599.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006514599.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_030245738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101598.1
    CDS65..7156
    Translation

    Target ORF information:

    RefSeq Version XM_030245738.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_006514600.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514600.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006514663.1
    CDS120..7211
    Translation

    Target ORF information:

    RefSeq Version XM_006514600.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006514600.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_030245736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101596.1
    CDS2701..9792
    Translation

    Target ORF information:

    RefSeq Version XM_030245736.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_030245737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030101597.1
    CDS64..7155
    Translation

    Target ORF information:

    RefSeq Version XM_030245737.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030245737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45140
    Clone ID Related Accession (Same CDS sequence) XM_006514601.3 , XM_006514601.2
    Accession Version XM_006514601.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006514601.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAGTTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
    GTGCCTTACA ACTACAACCA ACTGGAAGGA AGATTCAAAC AGCTCCAAGA TGAGCGTGAA
    GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCGAG AGTGTCCTGC
    CGAATCACAG ACCTGTACAC GGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGGAT CCACTGTCTG
    GAGAATGTCG ACAAGGCTCT TCAATTCCTG AAAGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACAATT
    ATTCTGCGCT TCCAGATCCA GGATATTAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG
    TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAT
    AAACATCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCTAATGC ACACTACAAT
    CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TCACTAAACT GTTAGACCCT
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATCA TCACATACGT GGTGACTTAC
    TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ACGAGTCACT TGCTTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATC ATCCTAAACA ACCGCAAATT TGCTAATTCA
    CTGGTTGGGG TCCAGCAGCA GCTCCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA
    CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTCCTTT TCACAATTCA GAGCAAGATG
    CGAGCGAATA ATCAGAAGGT CTACATGCCC CGCGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG
    CTCATACGGC AGGAAAAACT GGAACAACTC GCCCGAAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACAT GGCTGAGTGA AAACCAGCGT CTTGTGTCTC AGGACAACTT TGGATTTGAC
    CTTCCCGCTG TTGAGGCTGC TACCAAAAAA CACGAGGCCA TTGAGACAGA CATCGCTGCA
    TATGAAGAAC GAGTTCAGGC CGTGGTGGCT GTGGCCAGGG AACTTGAAGC CGAGAACTAC
    CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACTTG
    CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGATT GCAAAAGATA
    TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTT GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGCAATCCA AGCAGAGCGT GTAAGAGGTG TGAATGCCTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG CTACAAGCCA TGTGACCCCC AGGTAATTCG AGACCGTGTT
    GCCCACATGG AGTTCTGCTA TCAAGAGCTT TGTCAGCTGG CTGCCGAGCG TAGGGCTCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGAGAGA AGGAGAAGAT CCTGTCCTCT GATGATTACG GGAAAGACTT GACCAGTGTC
    ATGCGCCTGC TGAGCAAGCA CCGGGCATTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT
    TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCGGAA
    AAGATCCGTG AGAGAATCAT TTATATCCGG GAGCAGTGGG CCAACCTGGA ACAGCTCTCA
    GCCATTAGGA AGAAGCGCCT AGAGGAAGCC TCATTACTGC ACCAGTTCCA GGCTGATGCT
    GATGATATTG ATGCTTGGAT GTTAGATATA CTCAAGATTG TCTCCAGCAA TGATGTGGGC
    CATGATGAGT ACTCCACGCA GTCTCTGGTC AAGAAGCATA AAGATGTAGC AGAAGAGATC
    ACCAACTACA GGCCCACTAT TGACACACTG CATGAGCAAG CCAGTGCCCT TCCACAAGCA
    CATGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG CTGCAAGGAG
    ATGGCAGAGT TAACACGGCT AAGGAAGCAG GCTCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG
    CAGCTGATGC ACAATGGCCA CCCCAGTGAA AAGGAAATCA GAGCTCAGCA AGACAAACTC
    AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AAAAGGATGC TCTTCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG
    AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAACGA GACTTGGTAG CCATTGAGGC GAAGCTGAGT
    GACCTGCAGA AAGAAGCTGA GAAGCTGGAG TCCGAGCACC CTGACCAGGC TCAAGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGATGTG TGGGAGGAAA TGAAGACAAC CCTGAAGAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TTCTGCGGGA CTTGGACGAC
    TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATCGCCT CAGAGGACAT GCCCAATACC
    CTCACTGAGG CAGAGAAGCT TCTCACACAG CACGAGAATA TCAAAAATGA GATCGACAAT
    TATGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACT
    GATGCCCAGT ATATGTTTCT GCGGCAGCGG CTGCAGGCCT TAGACACTGG CTGGAATGAG
    CTCCACAAAA TGTGGGAGAA CAGGCAAAAC CTCCTCTCCC AGTCCCATGC CTACCAGCAG
    TTCCTTAGGG ACACCAAACA AGCTGAAGCT TTTCTTAATA ACCAGGAGTA TGTTTTGGCT
    CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAAAA GCAGGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGA
    AGACTGGTGA GCGATGGGAA CATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT
    GACGACAGAC ACAGGAAGAA TCGAGAAGCA GCCAGTGAAC TTCTGATGAG GTTAAAGGAC
    AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA
    AAGATGCTTA CAGCTCAAGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAGCATC AAGCATTTAT GGCGGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAAGCTGT GGTAAAGGAA
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC
    CAGCGGCTCT TTGATGCAAA TAAGGCTGAG CTTTTCACAC AAAGCTGCGC AGATCTTGAC
    AAATGGCTAC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTTACC
    AGTGTCAATA TTCTTCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTTCGGAAG
    AAAGAGATCG AGGAACTGCA GAGCCAAGCC CAGGCGCTGA GTCAGGAGGG GAAGAGCACA
    GATGAGGTGG ACAGCAAACG CCTTACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC
    TTGAGTGAGA GGAAGCATAA CCTGTTAGCT TCCAAGGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AAATCCTATG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT
    GGCCATAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA
    ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGTCAAAA CATCATCACA
    GATAGCAGCA GCCTCAATGC CGAGGCTATC AGGCAGAGGC TCGCTGACCT GAAGCAGCTG
    TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTGGAGGA GGCACACAAG
    GCGCAGCAGT ACTACTTTGA TGCAGCTGAA GCCGAGGCAT GGATGAGTGA ACAGGAGTTG
    TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCTAT GTTGAAAAAG
    CACCAGATTT TAGAGCAAGC TGTTGAGGAC TATGCAGAGA CAGTACACCA GCTCTCCAAG
    ACTAGCCGGG CGCTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG
    TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGAAAA
    CTTGATGAGA GGCACAGGCT GTTCCAGCTC AACAGAGAGG TGGATGACCT GGAACAGTGG
    ATCGCTGAGA GGGAAGTGGT CGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG
    CGTGTGGATA CAGTTAATAA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCT
    GCCACCATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCTGCC TCATATGAAC TTCATAAGTT TTACCATGAT
    GCCAAGGAGA TCTTTGGCCG AATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA
    AGAGATCAAA ACACTGTGGA AACTTTACAG AGAATGCACA CCACCTTTGA GCACGACATC
    CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCTCGCCT CCAGGCAGCC
    TATGCAGGGG ACAAGGCTGA TGACATCCAG AAGCGTGAGA ATGAGGTCCT GGAAGCCTGG
    AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TGGTAGACAC AGGAGACAAG
    TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAACC ACGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT TGATGCTCGT AATGACAGCT TTACAGCCTG CATTGAGCTT
    GGGAAATCCC TGCTGGCACG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGCTGACAG AGAAAAGAAA AGAAATGATT GACAAGTGGG AAGACCGGTG GGAGTGGTTA
    AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGGGATGCCA GTGTGGCAGA GGCTTGGCTG
    CTTGGACAGG AACCATACCT ATCCAGCCGT GAAATTGGCC AGAGTGTAGA CGAAGTGGAG
    AAGCTTATTA AGCGCCATGA GGCGTTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGATTC
    TCTGCTCTGG AAAGGCTGAC AACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAAGAAAGAA AGAGGCGGCC ACCTTCTCCG GACCCAAACA CGAAGGTTTC AGAGGAGGCT
    GAGTCCCAGC AATGGGATAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAGCAGGGAT CTCCACGGAT GGCAGGAACC ATGGAAACGA GTGAAATGGT CAACGGTGCT
    GCTGAGCAGA GGACAAGCTC CAAAGAGTCC AGTCCTGTTC CCTCTCCCAC CTTGGACCGA
    AAGGCCAAAT CTGCACTTCC AGCCCAGAGT GCTGCCACCC TGCCAGCCAG GACCCTGGAG
    ACACCCGCTG CCCAGATGGA AGGCTTCCTC AATCGGAAGC ATGAGTGGGA GGCCCACAAT
    AAGAAAGCCT CGAGCAGGTC CTGGCACAAT GTATATTGTG TCATAAATAA CCAAGAAATG
    GGCTTCTATA AAGATGCCAA GAGTGCTGCT TCTGGCATCC CCTACCACAG TGAGGTCCCT
    GTGAGTTTGA AAGAGGCCAT CTGCGAAGTG GCCCTTGATT ACAAAAAGAA GAAGCACGTG
    TTCAAGCTAA GACTAAGTGA TGGAAACGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC TATCTCCTCT GCCATCTCCT CTGACAAACA CGACACATCT
    GCCAGCACCC AGAGTACGCC AGCATCCAGT CGGGCGCAGA CCTTACCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCTGGCAAG AGGGAGAAGG ATAAAGAGAA AGACAAAGAG
    AAGAGGTTCA GCCTTTTCGG CAAGAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006514664.1
    CDS768..7820
    Translation

    Target ORF information:

    RefSeq Version XM_006514601.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006514601.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAGTTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
    GTGCCTTACA ACTACAACCA ACTGGAAGGA AGATTCAAAC AGCTCCAAGA TGAGCGTGAA
    GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCGAG AGTGTCCTGC
    CGAATCACAG ACCTGTACAC GGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGGAT CCACTGTCTG
    GAGAATGTCG ACAAGGCTCT TCAATTCCTG AAAGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACAATT
    ATTCTGCGCT TCCAGATCCA GGATATTAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG
    TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAT
    AAACATCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCTAATGC ACACTACAAT
    CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TCACTAAACT GTTAGACCCT
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATCA TCACATACGT GGTGACTTAC
    TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ACGAGTCACT TGCTTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATC ATCCTAAACA ACCGCAAATT TGCTAATTCA
    CTGGTTGGGG TCCAGCAGCA GCTCCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA
    CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTCCTTT TCACAATTCA GAGCAAGATG
    CGAGCGAATA ATCAGAAGGT CTACATGCCC CGCGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG
    CTCATACGGC AGGAAAAACT GGAACAACTC GCCCGAAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACAT GGCTGAGTGA AAACCAGCGT CTTGTGTCTC AGGACAACTT TGGATTTGAC
    CTTCCCGCTG TTGAGGCTGC TACCAAAAAA CACGAGGCCA TTGAGACAGA CATCGCTGCA
    TATGAAGAAC GAGTTCAGGC CGTGGTGGCT GTGGCCAGGG AACTTGAAGC CGAGAACTAC
    CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACTTG
    CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGATT GCAAAAGATA
    TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTT GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGCAATCCA AGCAGAGCGT GTAAGAGGTG TGAATGCCTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG CTACAAGCCA TGTGACCCCC AGGTAATTCG AGACCGTGTT
    GCCCACATGG AGTTCTGCTA TCAAGAGCTT TGTCAGCTGG CTGCCGAGCG TAGGGCTCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGAGAGA AGGAGAAGAT CCTGTCCTCT GATGATTACG GGAAAGACTT GACCAGTGTC
    ATGCGCCTGC TGAGCAAGCA CCGGGCATTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT
    TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCGGAA
    AAGATCCGTG AGAGAATCAT TTATATCCGG GAGCAGTGGG CCAACCTGGA ACAGCTCTCA
    GCCATTAGGA AGAAGCGCCT AGAGGAAGCC TCATTACTGC ACCAGTTCCA GGCTGATGCT
    GATGATATTG ATGCTTGGAT GTTAGATATA CTCAAGATTG TCTCCAGCAA TGATGTGGGC
    CATGATGAGT ACTCCACGCA GTCTCTGGTC AAGAAGCATA AAGATGTAGC AGAAGAGATC
    ACCAACTACA GGCCCACTAT TGACACACTG CATGAGCAAG CCAGTGCCCT TCCACAAGCA
    CATGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG CTGCAAGGAG
    ATGGCAGAGT TAACACGGCT AAGGAAGCAG GCTCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG
    CAGCTGATGC ACAATGGCCA CCCCAGTGAA AAGGAAATCA GAGCTCAGCA AGACAAACTC
    AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AAAAGGATGC TCTTCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG
    AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAACGA GACTTGGTAG CCATTGAGGC GAAGCTGAGT
    GACCTGCAGA AAGAAGCTGA GAAGCTGGAG TCCGAGCACC CTGACCAGGC TCAAGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGATGTG TGGGAGGAAA TGAAGACAAC CCTGAAGAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TTCTGCGGGA CTTGGACGAC
    TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATCGCCT CAGAGGACAT GCCCAATACC
    CTCACTGAGG CAGAGAAGCT TCTCACACAG CACGAGAATA TCAAAAATGA GATCGACAAT
    TATGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACT
    GATGCCCAGT ATATGTTTCT GCGGCAGCGG CTGCAGGCCT TAGACACTGG CTGGAATGAG
    CTCCACAAAA TGTGGGAGAA CAGGCAAAAC CTCCTCTCCC AGTCCCATGC CTACCAGCAG
    TTCCTTAGGG ACACCAAACA AGCTGAAGCT TTTCTTAATA ACCAGGAGTA TGTTTTGGCT
    CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAAAA GCAGGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGA
    AGACTGGTGA GCGATGGGAA CATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT
    GACGACAGAC ACAGGAAGAA TCGAGAAGCA GCCAGTGAAC TTCTGATGAG GTTAAAGGAC
    AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA
    AAGATGCTTA CAGCTCAAGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAGCATC AAGCATTTAT GGCGGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAAGCTGT GGTAAAGGAA
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC
    CAGCGGCTCT TTGATGCAAA TAAGGCTGAG CTTTTCACAC AAAGCTGCGC AGATCTTGAC
    AAATGGCTAC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTTACC
    AGTGTCAATA TTCTTCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTTCGGAAG
    AAAGAGATCG AGGAACTGCA GAGCCAAGCC CAGGCGCTGA GTCAGGAGGG GAAGAGCACA
    GATGAGGTGG ACAGCAAACG CCTTACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC
    TTGAGTGAGA GGAAGCATAA CCTGTTAGCT TCCAAGGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AAATCCTATG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT
    GGCCATAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA
    ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGTCAAAA CATCATCACA
    GATAGCAGCA GCCTCAATGC CGAGGCTATC AGGCAGAGGC TCGCTGACCT GAAGCAGCTG
    TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTGGAGGA GGCACACAAG
    GCGCAGCAGT ACTACTTTGA TGCAGCTGAA GCCGAGGCAT GGATGAGTGA ACAGGAGTTG
    TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCTAT GTTGAAAAAG
    CACCAGATTT TAGAGCAAGC TGTTGAGGAC TATGCAGAGA CAGTACACCA GCTCTCCAAG
    ACTAGCCGGG CGCTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG
    TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGAAAA
    CTTGATGAGA GGCACAGGCT GTTCCAGCTC AACAGAGAGG TGGATGACCT GGAACAGTGG
    ATCGCTGAGA GGGAAGTGGT CGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG
    CGTGTGGATA CAGTTAATAA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCT
    GCCACCATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCTGCC TCATATGAAC TTCATAAGTT TTACCATGAT
    GCCAAGGAGA TCTTTGGCCG AATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA
    AGAGATCAAA ACACTGTGGA AACTTTACAG AGAATGCACA CCACCTTTGA GCACGACATC
    CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCTCGCCT CCAGGCAGCC
    TATGCAGGGG ACAAGGCTGA TGACATCCAG AAGCGTGAGA ATGAGGTCCT GGAAGCCTGG
    AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TGGTAGACAC AGGAGACAAG
    TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAACC ACGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT TGATGCTCGT AATGACAGCT TTACAGCCTG CATTGAGCTT
    GGGAAATCCC TGCTGGCACG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGCTGACAG AGAAAAGAAA AGAAATGATT GACAAGTGGG AAGACCGGTG GGAGTGGTTA
    AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGGGATGCCA GTGTGGCAGA GGCTTGGCTG
    CTTGGACAGG AACCATACCT ATCCAGCCGT GAAATTGGCC AGAGTGTAGA CGAAGTGGAG
    AAGCTTATTA AGCGCCATGA GGCGTTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGATTC
    TCTGCTCTGG AAAGGCTGAC AACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAAGAAAGAA AGAGGCGGCC ACCTTCTCCG GACCCAAACA CGAAGGTTTC AGAGGAGGCT
    GAGTCCCAGC AATGGGATAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAGCAGGGAT CTCCACGGAT GGCAGGAACC ATGGAAACGA GTGAAATGGT CAACGGTGCT
    GCTGAGCAGA GGACAAGCTC CAAAGAGTCC AGTCCTGTTC CCTCTCCCAC CTTGGACCGA
    AAGGCCAAAT CTGCACTTCC AGCCCAGAGT GCTGCCACCC TGCCAGCCAG GACCCTGGAG
    ACACCCGCTG CCCAGATGGA AGGCTTCCTC AATCGGAAGC ATGAGTGGGA GGCCCACAAT
    AAGAAAGCCT CGAGCAGGTC CTGGCACAAT GTATATTGTG TCATAAATAA CCAAGAAATG
    GGCTTCTATA AAGATGCCAA GAGTGCTGCT TCTGGCATCC CCTACCACAG TGAGGTCCCT
    GTGAGTTTGA AAGAGGCCAT CTGCGAAGTG GCCCTTGATT ACAAAAAGAA GAAGCACGTG
    TTCAAGCTAA GACTAAGTGA TGGAAACGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC TATCTCCTCT GCCATCTCCT CTGACAAACA CGACACATCT
    GCCAGCACCC AGAGTACGCC AGCATCCAGT CGGGCGCAGA CCTTACCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCTGGCAAG AGGGAGAAGG ATAAAGAGAA AGACAAAGAG
    AAGAGGTTCA GCCTTTTCGG CAAGAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22257
    Clone ID Related Accession (Same CDS sequence) NM_009260.2
    Accession Version NM_009260.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK169544.1, AK157727.1, CK627078.1, CF539329.1, AF017112.1, AK161119.1 and AK075675.1. On Nov 13, 2006 this sequence version replaced NM_009260.1. Transcript Variant: This variant (2) contains different segments for its 5' UTR, 5' coding region, 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 2) has shorter and distinct N- and C- termini when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF017112.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAGTTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
    GTGCCTTACA ACTACAACCA ACTGGAAGGA AGATTCAAAC AGCTCCAAGA TGAGCGTGAA
    GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCGAG AGTGTCCTGC
    CGAATCACAG ACCTGTACAC GGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGGAT CCACTGTCTG
    GAGAATGTCG ACAAGGCTCT TCAATTCCTG AAAGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACAATT
    ATTCTGCGCT TCCAGATCCA GGATATTAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG
    TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAT
    AAACATCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCTAATGC ACACTACAAT
    CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TCACTAAACT GTTAGACCCT
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATCA TCACATACGT GGTGACTTAC
    TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ACGAGTCACT TGCTTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATC ATCCTAAACA ACCGCAAATT TGCTAATTCA
    CTGGTTGGGG TCCAGCAGCA GCTCCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA
    CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTCCTTT TCACAATTCA GAGCAAGATG
    CGAGCGAATA ATCAGAAGGT CTACATGCCC CGCGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG
    CTCATACGGC AGGAAAAACT GGAACAACTC GCCCGAAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACAT GGCTGAGTGA AAACCAGCGT CTTGTGTCTC AGGACAACTT TGGATTTGAC
    CTTCCCGCTG TTGAGGCTGC TACCAAAAAA CACGAGGCCA TTGAGACAGA CATCGCTGCA
    TATGAAGAAC GAGTTCAGGC CGTGGTGGCT GTGGCCAGGG AACTTGAAGC CGAGAACTAC
    CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACTTG
    CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGATT GCAAAAGATA
    TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTT GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGCAATCCA AGCAGAGCGT GTAAGAGGTG TGAATGCCTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG CTACAAGCCA TGTGACCCCC AGGTAATTCG AGACCGTGTT
    GCCCACATGG AGTTCTGCTA TCAAGAGCTT TGTCAGCTGG CTGCCGAGCG TAGGGCTCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGAGAGA AGGAGAAGAT CCTGTCCTCT GATGATTACG GGAAAGACTT GACCAGTGTC
    ATGCGCCTGC TGAGCAAGCA CCGGGCATTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT
    TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCGGAA
    AAGATCCGTG AGAGAATCAT TTATATCCGG GAGCAGTGGG CCAACCTGGA ACAGCTCTCA
    GCCATTAGGA AGAAGCGCCT AGAGGAAGCC TCATTACTGC ACCAGTTCCA GGCTGATGCT
    GATGATATTG ATGCTTGGAT GTTAGATATA CTCAAGATTG TCTCCAGCAA TGATGTGGGC
    CATGATGAGT ACTCCACGCA GTCTCTGGTC AAGAAGCATA AAGATGTAGC AGAAGAGATC
    ACCAACTACA GGCCCACTAT TGACACACTG CATGAGCAAG CCAGTGCCCT TCCACAAGCA
    CATGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG CTGCAAGGAG
    ATGGCAGAGT TAACACGGCT AAGGAAGCAG GCTCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG
    CAGCTGATGC ACAATGGCCA CCCCAGTGAA AAGGAAATCA GAGCTCAGCA AGACAAACTC
    AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AAAAGGATGC TCTTCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG
    AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAACGA GACTTGGTAG CCATTGAGGC GAAGCTGAGT
    GACCTGCAGA AAGAAGCTGA GAAGCTGGAG TCCGAGCACC CTGACCAGGC TCAAGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGATGTG TGGGAGGAAA TGAAGACAAC CCTGAAGAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TTCTGCGGGA CTTGGACGAC
    TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATCGCCT CAGAGGACAT GCCCAATACC
    CTCACTGAGG CAGAGAAGCT TCTCACACAG CACGAGAATA TCAAAAATGA GATCGACAAT
    TATGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACT
    GATGCCCAGT ATATGTTTCT GCGGCAGCGG CTGCAGGCCT TAGACACTGG CTGGAATGAG
    CTCCACAAAA TGTGGGAGAA CAGGCAAAAC CTCCTCTCCC AGTCCCATGC CTACCAGCAG
    TTCCTTAGGG ACACCAAACA AGCTGAAGCT TTTCTTAATA ACCAGGAGTA TGTTTTGGCT
    CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAAAA GCAGGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGA
    AGACTGGTGA GCGATGGGAA CATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT
    GACGACAGAC ACAGGAAGAA TCGAGAAGCA GCCAGTGAAC TTCTGATGAG GTTAAAGGAC
    AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA
    AAGATGCTTA CAGCTCAAGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAGCATC AAGCATTTAT GGCGGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAAGCTGT GGTAAAGGAA
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC
    CAGCGGCTCT TTGATGCAAA TAAGGCTGAG CTTTTCACAC AAAGCTGCGC AGATCTTGAC
    AAATGGCTAC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTTACC
    AGTGTCAATA TTCTTCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTTCGGAAG
    AAAGAGATCG AGGAACTGCA GAGCCAAGCC CAGGCGCTGA GTCAGGAGGG GAAGAGCACA
    GATGAGGTGG ACAGCAAACG CCTTACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC
    TTGAGTGAGA GGAAGCATAA CCTGTTAGCT TCCAAGGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AAATCCTATG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT
    GGCCATAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA
    ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGTCAAAA CATCATCACA
    GATAGCAGCA GCCTCAATGC CGAGGCTATC AGGCAGAGGC TCGCTGACCT GAAGCAGCTG
    TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTGGAGGA GGCACACAAG
    GCGCAGCAGT ACTACTTTGA TGCAGCTGAA GCCGAGGCAT GGATGAGTGA ACAGGAGTTG
    TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCTAT GTTGAAAAAG
    CACCAGATTT TAGAGCAAGC TGTTGAGGAC TATGCAGAGA CAGTACACCA GCTCTCCAAG
    ACTAGCCGGG CGCTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG
    TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGAAAA
    CTTGATGAGA GGCACAGGCT GTTCCAGCTC AACAGAGAGG TGGATGACCT GGAACAGTGG
    ATCGCTGAGA GGGAAGTGGT CGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG
    CGTGTGGATA CAGTTAATAA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCT
    GCCACCATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCTGCC TCATATGAAC TTCATAAGTT TTACCATGAT
    GCCAAGGAGA TCTTTGGCCG AATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA
    AGAGATCAAA ACACTGTGGA AACTTTACAG AGAATGCACA CCACCTTTGA GCACGACATC
    CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCTCGCCT CCAGGCAGCC
    TATGCAGGGG ACAAGGCTGA TGACATCCAG AAGCGTGAGA ATGAGGTCCT GGAAGCCTGG
    AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TGGTAGACAC AGGAGACAAG
    TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAACC ACGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT TGATGCTCGT AATGACAGCT TTACAGCCTG CATTGAGCTT
    GGGAAATCCC TGCTGGCACG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGCTGACAG AGAAAAGAAA AGAAATGATT GACAAGTGGG AAGACCGGTG GGAGTGGTTA
    AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGGGATGCCA GTGTGGCAGA GGCTTGGCTG
    CTTGGACAGG AACCATACCT ATCCAGCCGT GAAATTGGCC AGAGTGTAGA CGAAGTGGAG
    AAGCTTATTA AGCGCCATGA GGCGTTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGATTC
    TCTGCTCTGG AAAGGCTGAC AACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAAGAAAGAA AGAGGCGGCC ACCTTCTCCG GACCCAAACA CGAAGGTTTC AGAGGAGGCT
    GAGTCCCAGC AATGGGATAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAGCAGGGAT CTCCACGGGT TAGTTACCGC TCTCAAACGT ACCAAAACTA CAAAAACTTT
    AATAGCAGAC GGACAGCCAG TGACCATTCA TGGTCTGGAA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_033286.2
    CDS426..6890
    Translation

    Target ORF information:

    RefSeq Version NM_009260.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_009260.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAGTTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
    GTGCCTTACA ACTACAACCA ACTGGAAGGA AGATTCAAAC AGCTCCAAGA TGAGCGTGAA
    GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCGAG AGTGTCCTGC
    CGAATCACAG ACCTGTACAC GGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGGAT CCACTGTCTG
    GAGAATGTCG ACAAGGCTCT TCAATTCCTG AAAGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACAATT
    ATTCTGCGCT TCCAGATCCA GGATATTAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG
    TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAT
    AAACATCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCTAATGC ACACTACAAT
    CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TCACTAAACT GTTAGACCCT
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATCA TCACATACGT GGTGACTTAC
    TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ACGAGTCACT TGCTTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATC ATCCTAAACA ACCGCAAATT TGCTAATTCA
    CTGGTTGGGG TCCAGCAGCA GCTCCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA
    CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTCCTTT TCACAATTCA GAGCAAGATG
    CGAGCGAATA ATCAGAAGGT CTACATGCCC CGCGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG
    CTCATACGGC AGGAAAAACT GGAACAACTC GCCCGAAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACAT GGCTGAGTGA AAACCAGCGT CTTGTGTCTC AGGACAACTT TGGATTTGAC
    CTTCCCGCTG TTGAGGCTGC TACCAAAAAA CACGAGGCCA TTGAGACAGA CATCGCTGCA
    TATGAAGAAC GAGTTCAGGC CGTGGTGGCT GTGGCCAGGG AACTTGAAGC CGAGAACTAC
    CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACTTG
    CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGATT GCAAAAGATA
    TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTT GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGCAATCCA AGCAGAGCGT GTAAGAGGTG TGAATGCCTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG CTACAAGCCA TGTGACCCCC AGGTAATTCG AGACCGTGTT
    GCCCACATGG AGTTCTGCTA TCAAGAGCTT TGTCAGCTGG CTGCCGAGCG TAGGGCTCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGAGAGA AGGAGAAGAT CCTGTCCTCT GATGATTACG GGAAAGACTT GACCAGTGTC
    ATGCGCCTGC TGAGCAAGCA CCGGGCATTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT
    TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCGGAA
    AAGATCCGTG AGAGAATCAT TTATATCCGG GAGCAGTGGG CCAACCTGGA ACAGCTCTCA
    GCCATTAGGA AGAAGCGCCT AGAGGAAGCC TCATTACTGC ACCAGTTCCA GGCTGATGCT
    GATGATATTG ATGCTTGGAT GTTAGATATA CTCAAGATTG TCTCCAGCAA TGATGTGGGC
    CATGATGAGT ACTCCACGCA GTCTCTGGTC AAGAAGCATA AAGATGTAGC AGAAGAGATC
    ACCAACTACA GGCCCACTAT TGACACACTG CATGAGCAAG CCAGTGCCCT TCCACAAGCA
    CATGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG CTGCAAGGAG
    ATGGCAGAGT TAACACGGCT AAGGAAGCAG GCTCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG
    CAGCTGATGC ACAATGGCCA CCCCAGTGAA AAGGAAATCA GAGCTCAGCA AGACAAACTC
    AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AAAAGGATGC TCTTCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG
    AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAACGA GACTTGGTAG CCATTGAGGC GAAGCTGAGT
    GACCTGCAGA AAGAAGCTGA GAAGCTGGAG TCCGAGCACC CTGACCAGGC TCAAGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGATGTG TGGGAGGAAA TGAAGACAAC CCTGAAGAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TTCTGCGGGA CTTGGACGAC
    TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATCGCCT CAGAGGACAT GCCCAATACC
    CTCACTGAGG CAGAGAAGCT TCTCACACAG CACGAGAATA TCAAAAATGA GATCGACAAT
    TATGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACT
    GATGCCCAGT ATATGTTTCT GCGGCAGCGG CTGCAGGCCT TAGACACTGG CTGGAATGAG
    CTCCACAAAA TGTGGGAGAA CAGGCAAAAC CTCCTCTCCC AGTCCCATGC CTACCAGCAG
    TTCCTTAGGG ACACCAAACA AGCTGAAGCT TTTCTTAATA ACCAGGAGTA TGTTTTGGCT
    CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAAAA GCAGGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGA
    AGACTGGTGA GCGATGGGAA CATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT
    GACGACAGAC ACAGGAAGAA TCGAGAAGCA GCCAGTGAAC TTCTGATGAG GTTAAAGGAC
    AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA
    AAGATGCTTA CAGCTCAAGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAGCATC AAGCATTTAT GGCGGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAAGCTGT GGTAAAGGAA
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC
    CAGCGGCTCT TTGATGCAAA TAAGGCTGAG CTTTTCACAC AAAGCTGCGC AGATCTTGAC
    AAATGGCTAC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTTACC
    AGTGTCAATA TTCTTCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTTCGGAAG
    AAAGAGATCG AGGAACTGCA GAGCCAAGCC CAGGCGCTGA GTCAGGAGGG GAAGAGCACA
    GATGAGGTGG ACAGCAAACG CCTTACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC
    TTGAGTGAGA GGAAGCATAA CCTGTTAGCT TCCAAGGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AAATCCTATG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT
    GGCCATAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA
    ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGTCAAAA CATCATCACA
    GATAGCAGCA GCCTCAATGC CGAGGCTATC AGGCAGAGGC TCGCTGACCT GAAGCAGCTG
    TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTGGAGGA GGCACACAAG
    GCGCAGCAGT ACTACTTTGA TGCAGCTGAA GCCGAGGCAT GGATGAGTGA ACAGGAGTTG
    TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCTAT GTTGAAAAAG
    CACCAGATTT TAGAGCAAGC TGTTGAGGAC TATGCAGAGA CAGTACACCA GCTCTCCAAG
    ACTAGCCGGG CGCTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG
    TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGAAAA
    CTTGATGAGA GGCACAGGCT GTTCCAGCTC AACAGAGAGG TGGATGACCT GGAACAGTGG
    ATCGCTGAGA GGGAAGTGGT CGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG
    CGTGTGGATA CAGTTAATAA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCT
    GCCACCATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCTGCC TCATATGAAC TTCATAAGTT TTACCATGAT
    GCCAAGGAGA TCTTTGGCCG AATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA
    AGAGATCAAA ACACTGTGGA AACTTTACAG AGAATGCACA CCACCTTTGA GCACGACATC
    CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCTCGCCT CCAGGCAGCC
    TATGCAGGGG ACAAGGCTGA TGACATCCAG AAGCGTGAGA ATGAGGTCCT GGAAGCCTGG
    AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TGGTAGACAC AGGAGACAAG
    TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAACC ACGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT TGATGCTCGT AATGACAGCT TTACAGCCTG CATTGAGCTT
    GGGAAATCCC TGCTGGCACG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGCTGACAG AGAAAAGAAA AGAAATGATT GACAAGTGGG AAGACCGGTG GGAGTGGTTA
    AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGGGATGCCA GTGTGGCAGA GGCTTGGCTG
    CTTGGACAGG AACCATACCT ATCCAGCCGT GAAATTGGCC AGAGTGTAGA CGAAGTGGAG
    AAGCTTATTA AGCGCCATGA GGCGTTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGATTC
    TCTGCTCTGG AAAGGCTGAC AACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAAGAAAGAA AGAGGCGGCC ACCTTCTCCG GACCCAAACA CGAAGGTTTC AGAGGAGGCT
    GAGTCCCAGC AATGGGATAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAGCAGGGAT CTCCACGGGT TAGTTACCGC TCTCAAACGT ACCAAAACTA CAAAAACTTT
    AATAGCAGAC GGACAGCCAG TGACCATTCA TGGTCTGGAA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version NM_175836.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-26
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL672225.15, AK169544.1, AK157727.1, CK627078.1, CF539329.1, AF017112.1, AK051630.1 and BC024833.1. On Nov 13, 2006 this sequence version replaced NM_175836.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_787030.2
    CDS47..7138
    Translation

    Target ORF information:

    RefSeq Version NM_175836.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175836.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_006514600.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006514663.1
    CDS351..7442
    Translation

    Target ORF information:

    RefSeq Version XM_006514600.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006514600.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu22354
    Clone ID Related Accession (Same CDS sequence) XM_006514599.4 , NM_175836.2 , XM_006514600.3 , XM_030245738.1 , XM_006514599.3 , XM_006514600.4 , XM_030245736.1 , XM_030245737.1
    Accession Version XM_006514599.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514599.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006514662.1
    CDS305..7396
    Translation

    Target ORF information:

    RefSeq Version XM_006514599.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006514599.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CGGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGTGAT 
    GTGAACAACC GCTGGGATGT GGATGACTGG GACAATGAGA ACAGCTCTGC AAGGCTCTTT
    GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCGAGA GTGTCCTGCC GAATCACAGA CCTGTACACG
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGGCTG
    CCTAAACCCA CTAAGGGACG GATGCGGATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT
    CAATTCCTGA AAGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACAATTA TTCTGCGCTT CCAGATCCAG
    GATATTAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG
    CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAATGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACATA AACATCGGCC TGACCTGATA
    GATTTTGATA AACTGAAGAA ATCTAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG
    GCAGAGCAGC ACCTTGGCCT CACTAAACTG TTAGACCCTG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATCAT CACATACGTG GTGACTTACT ACCACTACTT CTCCAAGATG
    AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGAAAGGTGC TTGATAATGC TATAGAAACA
    GAGAAAATGA TTGAGAAGTA CGAGTCACTT GCTTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATCA TCCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG
    CTCCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG
    AATTTGGAGG TGCTCCTTTT CACAATTCAG AGCAAGATGC GAGCGAATAA TCAGAAGGTC
    TACATGCCCC GCGAGGGGAA GCTCATCTCT GACATCAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG
    GAACAACTCG CCCGAAGATT TGATCGCAAG GCAGCTATGA GGGAGACATG GCTGAGTGAA
    AACCAGCGTC TTGTGTCTCA GGACAACTTT GGATTTGACC TTCCCGCTGT TGAGGCTGCT
    ACCAAAAAAC ACGAGGCCAT TGAGACAGAC ATCGCTGCAT ATGAAGAACG AGTTCAGGCC
    GTGGTGGCTG TGGCCAGGGA ACTTGAAGCC GAGAACTACC ATGACATCAA GCGCATCACA
    GCGAGGAAGG ACAATGTCAT CCGGCTCTGG GAATACTTGC TGGAACTGCT CAGGGCCAGG
    AGGCAGCGTC TTGAGATGAA CCTGGGATTG CAAAAGATAT TCCAGGAAAT GCTTTATATT
    ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AAGACTATGG CAAACACTTA
    CTTGGTGTTG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA
    GCAGAGCGTG TAAGAGGTGT GAATGCCTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGC
    TACAAGCCAT GTGACCCCCA GGTAATTCGA GACCGTGTTG CCCACATGGA GTTCTGCTAT
    CAAGAGCTTT GTCAGCTGGC TGCCGAGCGT AGGGCTCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAAGAG GAAGGCTGGA TACGAGAGAA GGAGAAGATC
    CTGTCCTCTG ATGATTACGG GAAAGACTTG ACCAGTGTCA TGCGCCTGCT GAGCAAGCAC
    CGGGCATTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA
    GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCGGAAA AGATCCGTGA GAGAATCATT
    TATATCCGGG AGCAGTGGGC CAACCTGGAA CAGCTCTCAG CCATTAGGAA GAAGCGCCTA
    GAGGAAGCCT CATTACTGCA CCAGTTCCAG GCTGATGCTG ATGATATTGA TGCTTGGATG
    TTAGATATAC TCAAGATTGT CTCCAGCAAT GATGTGGGCC ATGATGAGTA CTCCACGCAG
    TCTCTGGTCA AGAAGCATAA AGATGTAGCA GAAGAGATCA CCAACTACAG GCCCACTATT
    GACACACTGC ATGAGCAAGC CAGTGCCCTT CCACAAGCAC ATGCAGAGTC TCCAGATGTG
    AAGGGCCGGC TGGCAGGAAT TGAGGAGCGC TGCAAGGAGA TGGCAGAGTT AACACGGCTA
    AGGAAGCAGG CTCTGCAGGA CACCCTGGCC CTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC
    CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAATGGCCAC
    CCCAGTGAAA AGGAAATCAG AGCTCAGCAA GACAAACTCA ACACGAGGTG GAGTCAGTTC
    AGAGAACTGG TGGACAGGAA AAAGGATGCT CTTCTGTCTG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT
    ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAACGAG ACTTGGTAGC CATTGAGGCG AAGCTGAGTG ACCTGCAGAA AGAAGCTGAG
    AAGCTGGAGT CCGAGCACCC TGACCAGGCT CAAGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGATGTGT GGGAGGAAAT GAAGACAACC CTGAAGAACC GAGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT TCTGCGGGAC TTGGACGACT TCCAGTCTTG GCTCTCCAGG
    ACCCAGACTG CTATCGCCTC AGAGGACATG CCCAATACCC TCACTGAGGC AGAGAAGCTT
    CTCACACAGC ACGAGAATAT CAAAAATGAG ATCGACAATT ATGAGGAAGA CTACCAGAAG
    ATGCGGGACA TGGGCGAGAT GGTCACCCAG GGGCAGACTG ATGCCCAGTA TATGTTTCTG
    CGGCAGCGGC TGCAGGCCTT AGACACTGGC TGGAATGAGC TCCACAAAAT GTGGGAGAAC
    AGGCAAAACC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACCAAACAA
    GCTGAAGCTT TTCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC
    CTGGAAGGAG CTGAAGCAGC CATTAAAAAG CAGGAGGACT TCATGACCAC CATGGATGCC
    AACGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGAA GACTGGTGAG CGATGGGAAC
    ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ACGACAGACA CAGGAAGAAT
    CGAGAAGCAG CCAGTGAACT TCTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC
    CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAAGAC
    ATGTCTTATG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG
    GCGGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT
    ATTTCAGAAA AGCCAGAAAC AGAAGCTGTG GTAAAGGAAA AACTCACTGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGCGGCTCTT TGATGCAAAT
    AAGGCTGAGC TTTTCACACA AAGCTGCGCA GATCTTGACA AATGGCTACA TGGCCTGGAG
    AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTTACCA GTGTCAATAT TCTTCTGAAA
    AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTTCGGAAGA AAGAGATCGA GGAACTGCAG
    AGCCAAGCCC AGGCGCTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC
    CTTACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAGTGAGAG GAAGCATAAC
    CTGTTAGCTT CCAAGGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA AATCCTATGG
    GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCATAACCT TCAAACTGTG
    CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT
    ATTGATGACA TCTTTGAGAG GAGTCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC
    GAGGCTATCA GGCAGAGGCT CGCTGACCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA
    ACTGAGAAAC GCCATAGACG GCTGGAGGAG GCACACAAGG CGCAGCAGTA CTACTTTGAT
    GCAGCTGAAG CCGAGGCATG GATGAGTGAA CAGGAGTTGT ACATGATGTC TGAGGAAAAG
    GCCAAGGATG AGCAGAGTGC TGTCTCTATG TTGAAAAAGC ACCAGATTTT AGAGCAAGCT
    GTTGAGGACT ATGCAGAGAC AGTACACCAG CTCTCCAAGA CTAGCCGGGC GCTGGTGGCT
    GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT
    GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGAAAAC TTGATGAGAG GCACAGGCTG
    TTCCAGCTCA ACAGAGAGGT GGATGACCTG GAACAGTGGA TCGCTGAGAG GGAAGTGGTC
    GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC
    CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTGGATAC AGTTAATAAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCTG CCACCATTGC TGAGTGGAAA
    GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACAAG AACACAGATT
    CTTGCTGCCT CATATGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGA
    ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAAAA CACTGTGGAA
    ACTTTACAGA GAATGCACAC CACCTTTGAG CACGACATCC AAGCTCTGGG CACTCAGGTG
    AGGCAGCTGC AGGAGGATGC AGCTCGCCTC CAGGCAGCCT ATGCAGGGGA CAAGGCTGAT
    GACATCCAGA AGCGTGAGAA TGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT
    GAGGGTCGCA GGGTGCGGCT GGTAGACACA GGAGACAAGT TCCGCTTCTT CAGCATGGTG
    CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAGA TCGAGGCCCA GGAGAAACCA
    CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGTATCAA AGCTGAAATT
    GATGCTCGTA ATGACAGCTT TACAGCCTGC ATTGAGCTTG GGAAATCCCT GCTGGCACGG
    AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGCTGACAGA GAAAAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGGTGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT
    CAGTTCTCAA GGGATGCCAG TGTGGCAGAG GCTTGGCTGC TTGGACAGGA ACCATACCTA
    TCCAGCCGTG AAATTGGCCA GAGTGTAGAC GAAGTGGAGA AGCTTATTAA GCGCCATGAG
    GCGTTTGAAA AGTCTGCAGC GACCTGGGAT GAGAGATTCT CTGCTCTGGA AAGGCTGACA
    ACGTTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGGCCA
    CCTTCTCCGG ACCCAAACAC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGATACT
    TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG
    GCAGGAACCA TGGAAACGAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC
    AAAGAGTCCA GTCCTGTTCC CTCTCCCACC TTGGACCGAA AGGCCAAATC TGCACTTCCA
    GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CACCCGCTGC CCAGATGGAA
    GGCTTCCTCA ATCGGAAGCA TGAGTGGGAG GCCCACAATA AGAAAGCCTC GAGCAGGTCC
    TGGCACAATG TATATTGTGT CATAAATAAC CAAGAAATGG GCTTCTATAA AGATGCCAAG
    AGTGCTGCTT CTGGCATCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC
    TGCGAAGTGG CCCTTGATTA CAAAAAGAAG AAGCACGTGT TCAAGCTAAG ACTAAGTGAT
    GGAAACGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCT
    ATCTCCTCTG CCATCTCCTC TGACAAACAC GACACATCTG CCAGCACCCA GAGTACGCCA
    GCATCCAGTC GGGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCTGGCAAGA GGGAGAAGGA TAAAGAGAAA GACAAAGAGA AGAGGTTCAG CCTTTTCGGC
    AAGAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu45140
    Clone ID Related Accession (Same CDS sequence) XM_006514601.3 , XM_006514601.2
    Accession Version XM_006514601.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000077.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006514601.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAGTTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
    GTGCCTTACA ACTACAACCA ACTGGAAGGA AGATTCAAAC AGCTCCAAGA TGAGCGTGAA
    GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCGAG AGTGTCCTGC
    CGAATCACAG ACCTGTACAC GGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGGAT CCACTGTCTG
    GAGAATGTCG ACAAGGCTCT TCAATTCCTG AAAGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACAATT
    ATTCTGCGCT TCCAGATCCA GGATATTAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG
    TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAT
    AAACATCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCTAATGC ACACTACAAT
    CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TCACTAAACT GTTAGACCCT
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATCA TCACATACGT GGTGACTTAC
    TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ACGAGTCACT TGCTTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATC ATCCTAAACA ACCGCAAATT TGCTAATTCA
    CTGGTTGGGG TCCAGCAGCA GCTCCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA
    CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTCCTTT TCACAATTCA GAGCAAGATG
    CGAGCGAATA ATCAGAAGGT CTACATGCCC CGCGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG
    CTCATACGGC AGGAAAAACT GGAACAACTC GCCCGAAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACAT GGCTGAGTGA AAACCAGCGT CTTGTGTCTC AGGACAACTT TGGATTTGAC
    CTTCCCGCTG TTGAGGCTGC TACCAAAAAA CACGAGGCCA TTGAGACAGA CATCGCTGCA
    TATGAAGAAC GAGTTCAGGC CGTGGTGGCT GTGGCCAGGG AACTTGAAGC CGAGAACTAC
    CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACTTG
    CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGATT GCAAAAGATA
    TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTT GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGCAATCCA AGCAGAGCGT GTAAGAGGTG TGAATGCCTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG CTACAAGCCA TGTGACCCCC AGGTAATTCG AGACCGTGTT
    GCCCACATGG AGTTCTGCTA TCAAGAGCTT TGTCAGCTGG CTGCCGAGCG TAGGGCTCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGAGAGA AGGAGAAGAT CCTGTCCTCT GATGATTACG GGAAAGACTT GACCAGTGTC
    ATGCGCCTGC TGAGCAAGCA CCGGGCATTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT
    TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCGGAA
    AAGATCCGTG AGAGAATCAT TTATATCCGG GAGCAGTGGG CCAACCTGGA ACAGCTCTCA
    GCCATTAGGA AGAAGCGCCT AGAGGAAGCC TCATTACTGC ACCAGTTCCA GGCTGATGCT
    GATGATATTG ATGCTTGGAT GTTAGATATA CTCAAGATTG TCTCCAGCAA TGATGTGGGC
    CATGATGAGT ACTCCACGCA GTCTCTGGTC AAGAAGCATA AAGATGTAGC AGAAGAGATC
    ACCAACTACA GGCCCACTAT TGACACACTG CATGAGCAAG CCAGTGCCCT TCCACAAGCA
    CATGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG CTGCAAGGAG
    ATGGCAGAGT TAACACGGCT AAGGAAGCAG GCTCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG
    CAGCTGATGC ACAATGGCCA CCCCAGTGAA AAGGAAATCA GAGCTCAGCA AGACAAACTC
    AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AAAAGGATGC TCTTCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG
    AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAACGA GACTTGGTAG CCATTGAGGC GAAGCTGAGT
    GACCTGCAGA AAGAAGCTGA GAAGCTGGAG TCCGAGCACC CTGACCAGGC TCAAGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGATGTG TGGGAGGAAA TGAAGACAAC CCTGAAGAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TTCTGCGGGA CTTGGACGAC
    TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATCGCCT CAGAGGACAT GCCCAATACC
    CTCACTGAGG CAGAGAAGCT TCTCACACAG CACGAGAATA TCAAAAATGA GATCGACAAT
    TATGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACT
    GATGCCCAGT ATATGTTTCT GCGGCAGCGG CTGCAGGCCT TAGACACTGG CTGGAATGAG
    CTCCACAAAA TGTGGGAGAA CAGGCAAAAC CTCCTCTCCC AGTCCCATGC CTACCAGCAG
    TTCCTTAGGG ACACCAAACA AGCTGAAGCT TTTCTTAATA ACCAGGAGTA TGTTTTGGCT
    CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAAAA GCAGGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGA
    AGACTGGTGA GCGATGGGAA CATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT
    GACGACAGAC ACAGGAAGAA TCGAGAAGCA GCCAGTGAAC TTCTGATGAG GTTAAAGGAC
    AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA
    AAGATGCTTA CAGCTCAAGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAGCATC AAGCATTTAT GGCGGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAAGCTGT GGTAAAGGAA
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC
    CAGCGGCTCT TTGATGCAAA TAAGGCTGAG CTTTTCACAC AAAGCTGCGC AGATCTTGAC
    AAATGGCTAC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTTACC
    AGTGTCAATA TTCTTCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTTCGGAAG
    AAAGAGATCG AGGAACTGCA GAGCCAAGCC CAGGCGCTGA GTCAGGAGGG GAAGAGCACA
    GATGAGGTGG ACAGCAAACG CCTTACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC
    TTGAGTGAGA GGAAGCATAA CCTGTTAGCT TCCAAGGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AAATCCTATG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT
    GGCCATAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA
    ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGTCAAAA CATCATCACA
    GATAGCAGCA GCCTCAATGC CGAGGCTATC AGGCAGAGGC TCGCTGACCT GAAGCAGCTG
    TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTGGAGGA GGCACACAAG
    GCGCAGCAGT ACTACTTTGA TGCAGCTGAA GCCGAGGCAT GGATGAGTGA ACAGGAGTTG
    TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCTAT GTTGAAAAAG
    CACCAGATTT TAGAGCAAGC TGTTGAGGAC TATGCAGAGA CAGTACACCA GCTCTCCAAG
    ACTAGCCGGG CGCTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG
    TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGAAAA
    CTTGATGAGA GGCACAGGCT GTTCCAGCTC AACAGAGAGG TGGATGACCT GGAACAGTGG
    ATCGCTGAGA GGGAAGTGGT CGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG
    CGTGTGGATA CAGTTAATAA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCT
    GCCACCATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCTGCC TCATATGAAC TTCATAAGTT TTACCATGAT
    GCCAAGGAGA TCTTTGGCCG AATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA
    AGAGATCAAA ACACTGTGGA AACTTTACAG AGAATGCACA CCACCTTTGA GCACGACATC
    CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCTCGCCT CCAGGCAGCC
    TATGCAGGGG ACAAGGCTGA TGACATCCAG AAGCGTGAGA ATGAGGTCCT GGAAGCCTGG
    AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TGGTAGACAC AGGAGACAAG
    TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAACC ACGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT TGATGCTCGT AATGACAGCT TTACAGCCTG CATTGAGCTT
    GGGAAATCCC TGCTGGCACG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGCTGACAG AGAAAAGAAA AGAAATGATT GACAAGTGGG AAGACCGGTG GGAGTGGTTA
    AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGGGATGCCA GTGTGGCAGA GGCTTGGCTG
    CTTGGACAGG AACCATACCT ATCCAGCCGT GAAATTGGCC AGAGTGTAGA CGAAGTGGAG
    AAGCTTATTA AGCGCCATGA GGCGTTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGATTC
    TCTGCTCTGG AAAGGCTGAC AACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAAGAAAGAA AGAGGCGGCC ACCTTCTCCG GACCCAAACA CGAAGGTTTC AGAGGAGGCT
    GAGTCCCAGC AATGGGATAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAGCAGGGAT CTCCACGGAT GGCAGGAACC ATGGAAACGA GTGAAATGGT CAACGGTGCT
    GCTGAGCAGA GGACAAGCTC CAAAGAGTCC AGTCCTGTTC CCTCTCCCAC CTTGGACCGA
    AAGGCCAAAT CTGCACTTCC AGCCCAGAGT GCTGCCACCC TGCCAGCCAG GACCCTGGAG
    ACACCCGCTG CCCAGATGGA AGGCTTCCTC AATCGGAAGC ATGAGTGGGA GGCCCACAAT
    AAGAAAGCCT CGAGCAGGTC CTGGCACAAT GTATATTGTG TCATAAATAA CCAAGAAATG
    GGCTTCTATA AAGATGCCAA GAGTGCTGCT TCTGGCATCC CCTACCACAG TGAGGTCCCT
    GTGAGTTTGA AAGAGGCCAT CTGCGAAGTG GCCCTTGATT ACAAAAAGAA GAAGCACGTG
    TTCAAGCTAA GACTAAGTGA TGGAAACGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC TATCTCCTCT GCCATCTCCT CTGACAAACA CGACACATCT
    GCCAGCACCC AGAGTACGCC AGCATCCAGT CGGGCGCAGA CCTTACCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCTGGCAAG AGGGAGAAGG ATAAAGAGAA AGACAAAGAG
    AAGAGGTTCA GCCTTTTCGG CAAGAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006514664.1
    CDS817..7869
    Translation

    Target ORF information:

    RefSeq Version XM_006514601.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus spectrin beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006514601.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAGTTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
    GTGCCTTACA ACTACAACCA ACTGGAAGGA AGATTCAAAC AGCTCCAAGA TGAGCGTGAA
    GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCGAG AGTGTCCTGC
    CGAATCACAG ACCTGTACAC GGACCTTCGA GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGGAT CCACTGTCTG
    GAGAATGTCG ACAAGGCTCT TCAATTCCTG AAAGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACAATT
    ATTCTGCGCT TCCAGATCCA GGATATTAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG
    TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAATGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAT
    AAACATCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCTAATGC ACACTACAAT
    CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TCACTAAACT GTTAGACCCT
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATCA TCACATACGT GGTGACTTAC
    TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ACGAGTCACT TGCTTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATC ATCCTAAACA ACCGCAAATT TGCTAATTCA
    CTGGTTGGGG TCCAGCAGCA GCTCCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA
    CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTCCTTT TCACAATTCA GAGCAAGATG
    CGAGCGAATA ATCAGAAGGT CTACATGCCC CGCGAGGGGA AGCTCATCTC TGACATCAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG
    CTCATACGGC AGGAAAAACT GGAACAACTC GCCCGAAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACAT GGCTGAGTGA AAACCAGCGT CTTGTGTCTC AGGACAACTT TGGATTTGAC
    CTTCCCGCTG TTGAGGCTGC TACCAAAAAA CACGAGGCCA TTGAGACAGA CATCGCTGCA
    TATGAAGAAC GAGTTCAGGC CGTGGTGGCT GTGGCCAGGG AACTTGAAGC CGAGAACTAC
    CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGGCTCTG GGAATACTTG
    CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGATT GCAAAAGATA
    TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT
    CAAGACTATG GCAAACACTT ACTTGGTGTT GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGCAATCCA AGCAGAGCGT GTAAGAGGTG TGAATGCCTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG CTACAAGCCA TGTGACCCCC AGGTAATTCG AGACCGTGTT
    GCCCACATGG AGTTCTGCTA TCAAGAGCTT TGTCAGCTGG CTGCCGAGCG TAGGGCTCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAAGA GGAAGGCTGG
    ATACGAGAGA AGGAGAAGAT CCTGTCCTCT GATGATTACG GGAAAGACTT GACCAGTGTC
    ATGCGCCTGC TGAGCAAGCA CCGGGCATTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT
    TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCGGAA
    AAGATCCGTG AGAGAATCAT TTATATCCGG GAGCAGTGGG CCAACCTGGA ACAGCTCTCA
    GCCATTAGGA AGAAGCGCCT AGAGGAAGCC TCATTACTGC ACCAGTTCCA GGCTGATGCT
    GATGATATTG ATGCTTGGAT GTTAGATATA CTCAAGATTG TCTCCAGCAA TGATGTGGGC
    CATGATGAGT ACTCCACGCA GTCTCTGGTC AAGAAGCATA AAGATGTAGC AGAAGAGATC
    ACCAACTACA GGCCCACTAT TGACACACTG CATGAGCAAG CCAGTGCCCT TCCACAAGCA
    CATGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG CTGCAAGGAG
    ATGGCAGAGT TAACACGGCT AAGGAAGCAG GCTCTGCAGG ACACCCTGGC CCTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC
    CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG
    CAGCTGATGC ACAATGGCCA CCCCAGTGAA AAGGAAATCA GAGCTCAGCA AGACAAACTC
    AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AAAAGGATGC TCTTCTGTCT
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG
    AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAACGA GACTTGGTAG CCATTGAGGC GAAGCTGAGT
    GACCTGCAGA AAGAAGCTGA GAAGCTGGAG TCCGAGCACC CTGACCAGGC TCAAGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGATGTG TGGGAGGAAA TGAAGACAAC CCTGAAGAAC
    CGAGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TTCTGCGGGA CTTGGACGAC
    TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATCGCCT CAGAGGACAT GCCCAATACC
    CTCACTGAGG CAGAGAAGCT TCTCACACAG CACGAGAATA TCAAAAATGA GATCGACAAT
    TATGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACCCA GGGGCAGACT
    GATGCCCAGT ATATGTTTCT GCGGCAGCGG CTGCAGGCCT TAGACACTGG CTGGAATGAG
    CTCCACAAAA TGTGGGAGAA CAGGCAAAAC CTCCTCTCCC AGTCCCATGC CTACCAGCAG
    TTCCTTAGGG ACACCAAACA AGCTGAAGCT TTTCTTAATA ACCAGGAGTA TGTTTTGGCT
    CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAAAA GCAGGAGGAC
    TTCATGACCA CCATGGATGC CAACGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGA
    AGACTGGTGA GCGATGGGAA CATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT
    GACGACAGAC ACAGGAAGAA TCGAGAAGCA GCCAGTGAAC TTCTGATGAG GTTAAAGGAC
    AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA
    AAGATGCTTA CAGCTCAAGA CATGTCTTAT GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTAAAGCATC AAGCATTTAT GGCGGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAAGCTGT GGTAAAGGAA
    AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC
    CAGCGGCTCT TTGATGCAAA TAAGGCTGAG CTTTTCACAC AAAGCTGCGC AGATCTTGAC
    AAATGGCTAC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTTACC
    AGTGTCAATA TTCTTCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTTCGGAAG
    AAAGAGATCG AGGAACTGCA GAGCCAAGCC CAGGCGCTGA GTCAGGAGGG GAAGAGCACA
    GATGAGGTGG ACAGCAAACG CCTTACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC
    TTGAGTGAGA GGAAGCATAA CCTGTTAGCT TCCAAGGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AAATCCTATG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT
    GGCCATAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA
    ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGTCAAAA CATCATCACA
    GATAGCAGCA GCCTCAATGC CGAGGCTATC AGGCAGAGGC TCGCTGACCT GAAGCAGCTG
    TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTGGAGGA GGCACACAAG
    GCGCAGCAGT ACTACTTTGA TGCAGCTGAA GCCGAGGCAT GGATGAGTGA ACAGGAGTTG
    TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCTAT GTTGAAAAAG
    CACCAGATTT TAGAGCAAGC TGTTGAGGAC TATGCAGAGA CAGTACACCA GCTCTCCAAG
    ACTAGCCGGG CGCTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG
    TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGAAAA
    CTTGATGAGA GGCACAGGCT GTTCCAGCTC AACAGAGAGG TGGATGACCT GGAACAGTGG
    ATCGCTGAGA GGGAAGTGGT CGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC
    ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG
    CGTGTGGATA CAGTTAATAA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCT
    GCCACCATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC
    ATTGACACAA GAACACAGAT TCTTGCTGCC TCATATGAAC TTCATAAGTT TTACCATGAT
    GCCAAGGAGA TCTTTGGCCG AATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA
    AGAGATCAAA ACACTGTGGA AACTTTACAG AGAATGCACA CCACCTTTGA GCACGACATC
    CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCTCGCCT CCAGGCAGCC
    TATGCAGGGG ACAAGGCTGA TGACATCCAG AAGCGTGAGA ATGAGGTCCT GGAAGCCTGG
    AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TGGTAGACAC AGGAGACAAG
    TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAG
    ATCGAGGCCC AGGAGAAACC ACGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT
    CAAGGTATCA AAGCTGAAAT TGATGCTCGT AATGACAGCT TTACAGCCTG CATTGAGCTT
    GGGAAATCCC TGCTGGCACG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGCTGACAG AGAAAAGAAA AGAAATGATT GACAAGTGGG AAGACCGGTG GGAGTGGTTA
    AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGGGATGCCA GTGTGGCAGA GGCTTGGCTG
    CTTGGACAGG AACCATACCT ATCCAGCCGT GAAATTGGCC AGAGTGTAGA CGAAGTGGAG
    AAGCTTATTA AGCGCCATGA GGCGTTTGAA AAGTCTGCAG CGACCTGGGA TGAGAGATTC
    TCTGCTCTGG AAAGGCTGAC AACGTTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA
    GAAGAAAGAA AGAGGCGGCC ACCTTCTCCG GACCCAAACA CGAAGGTTTC AGAGGAGGCT
    GAGTCCCAGC AATGGGATAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAGCAGGGAT CTCCACGGAT GGCAGGAACC ATGGAAACGA GTGAAATGGT CAACGGTGCT
    GCTGAGCAGA GGACAAGCTC CAAAGAGTCC AGTCCTGTTC CCTCTCCCAC CTTGGACCGA
    AAGGCCAAAT CTGCACTTCC AGCCCAGAGT GCTGCCACCC TGCCAGCCAG GACCCTGGAG
    ACACCCGCTG CCCAGATGGA AGGCTTCCTC AATCGGAAGC ATGAGTGGGA GGCCCACAAT
    AAGAAAGCCT CGAGCAGGTC CTGGCACAAT GTATATTGTG TCATAAATAA CCAAGAAATG
    GGCTTCTATA AAGATGCCAA GAGTGCTGCT TCTGGCATCC CCTACCACAG TGAGGTCCCT
    GTGAGTTTGA AAGAGGCCAT CTGCGAAGTG GCCCTTGATT ACAAAAAGAA GAAGCACGTG
    TTCAAGCTAA GACTAAGTGA TGGAAACGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC TATCTCCTCT GCCATCTCCT CTGACAAACA CGACACATCT
    GCCAGCACCC AGAGTACGCC AGCATCCAGT CGGGCGCAGA CCTTACCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCTGGCAAG AGGGAGAAGG ATAAAGAGAA AGACAAAGAG
    AAGAGGTTCA GCCTTTTCGG CAAGAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.