Sptbn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sptbn1
Entrez Gene ID	305614
Full Name	spectrin, beta, non-erythrocytic 1
Synonyms	Spnb1,Spnb2
General protein information	Preferred Names spectrin, beta, non-erythrocytic 1 Names spectrin beta chain, non-erythrocytic 1 bSPII- beta II spectrin-like (short form) cytoskeleton protein non-erythroid spectrin beta short form of beta II spectrin spectrin beta 2 spectrin beta chain, brain 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22
Summary	a short form of beta II spectrin that lacks a pleckstrin homology domain and has a different C-terminal sequence; subcellular localization differs from that of the long form [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008770460.2	XP_008768682.1	spectrin beta chain, non-erythrocytic 1 isoform X2
XM_008770461.2	XP_008768683.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_008770458.2	XP_008768680.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_017599259.1	XP_017454748.1	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_008770463.2	XP_008768685.1	spectrin beta chain, non-erythrocytic 1 isoform X4
NM_001013130.1	NP_001013148.1	spectrin beta chain, non-erythrocytic 1
XM_008770462.2	XP_008768684.1	spectrin beta chain, non-erythrocytic 1 isoform X3

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPTBN1	NP_001186354.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010895	XP_309796.2
Caenorhabditis elegans (roundworm)	unc-70	NP_001024053.1
Canis lupus familiaris (dog)	SPTBN1	XP_531827.2
Pan troglodytes (chimpanzee)	SPTBN1	XP_001154155.1
	SPTBN1	XP_001114804.1
Bos taurus (cattle)	SPTBN1	NP_001179205.1
Drosophila melanogaster (fruit fly)	beta-Spec	NP_001259660.1
Homo sapiens (human)	SPTBN1	NP_003119.2
Mus musculus (house mouse)	Sptbn1	NP_787030.2
Rattus norvegicus (Norway rat)	Sptbn1	NP_001013148.1
Danio rerio (zebrafish)	sptb	NP_571600.1

Gene Ontology
Bibliography

Related articles in PubMed

Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.
Sidenstein SC, D'Este E, B?hm MJ, Danzl JG, Belov VN, Hell SW
Scientific reports626725(2016 May)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton.
Forster F, Paster W, Supper V, Schatzlmaier P, Sunzenauer S, Ostler N, Saliba A, Eckerstorfer P, Britzen-Laurent N, Sch?tz G, Schmid JA, Zlabinger GJ, Naschberger E, St?rzl M, Stockinger H
Journal of immunology (Baltimore, Md. : 1950)192(2)771-81(2014 Jan)

Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons.
Xu K, Zhong G, Zhuang X
Science (New York, N.Y.)339(6118)452-6(2013 Jan)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study of the organization of actin, spectrin and associated proteins in neurons; spectrin exhibited periodic structures alternating with those of actin and adducin; the distance between adjacent actin-adducin rings was comparable to the length of a spectrin tetramer
Title: Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons.

The following Sptbn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sptbn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03480	NM_001013130.1 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa46315	XM_008770461.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa46315	XM_017599259.1 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa46315	XM_008770458.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa46316	XM_008770460.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
ORa46317	XM_008770462.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa46318	XM_008770463.2 Latest version!	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sptbn1 ( NM_001013130.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013148.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), mRNA.

Price & Availability↑

CloneID	ORa03480
Clone ID Related Accession (Same CDS sequence)	NM_001013130.1
Accession Version	NM_001013130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-03
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY238344.1. On Apr 20, 2005 this sequence version replaced XM_240072.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY238344.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA CTAGCGCCCA AAACAAAGTT 
CTAGATGGAG CCTCTGTGTT CCTTCTGATG TACTCTTGCC CCCCCAGATG CTGGAGAATC 
AGATGGAAGA GGGGAAGAGC ACAGATGAGG GTGGACAGCA AACGCCTCAC TGTGCAGACC 
AAGTTCATGG AGCTTCTGGA GCCCTTGAAT GAAAGGAAGC ATAATCTGCT GGCTTCCAAG 
GAGATCCACC AGTTCAACAG GGATGTAGAG GACGAGATTC TGTGGGTTGG CGAGAGGATG 
CCTTTGGCAA CTTCCACAGA TCATGGCCAC AACCTTCAAA CTGTGCAGCT GTTAATAAAG 
AAAAACCAGA CCCTCCAGAA AGAAATCCAG GGACACCAGC CTCGTATTGA TGACATCTTT 
GAGAGGAGCC AAAACATCAT CACAGATAGC AGCAGCCTCA ATGCCGAGGC TATCAGACAG 
AGGCTCGCCG ATCTGAAGCA GCTGTGGGGG CTCCTCATTG AGGAAACTGA GAAACGCCAT 
AGACGGCTCG AGGAGGCACA CAAGGCCCAG CAATACTACT TTGATGCAGC TGAAGCAGAG 
GCATGGATGA GTGAACAGGA ACTGTACATG ATGTCTGAGG AAAAGGCCAA GGATGAGCAG 
AGTGCTGTCT CCATGTTGAA AAAGCACCAG ATTTTGGAAC AGGCTGTTGA GGACTATGCA 
GAGACAGTGC ACCAGCTCTC CAAGACCAGC CGGGCACTGG TGGCTGACAG CCATCCCGAA 
AGTGAGCGTA TTAGCATGCG GCAGTCAAAG GTCGACAAGC TGTATGCTGG CCTGAAGGAC 
CTTGCTGAGG AGAGGAGAGG GAAACTTGAT GAGAGGCACA GGCTATTCCA GCTCAACAGG 
GAGGTAGATG ACCTGGAACA GTGGATCGCC GAGAGGGAAG TGGTTGCAGG CTCCCATGAG 
TTGGGACAGG ACTATGAGCA TGTCACGATG TTACAAGAAC GGTTCCGAGA ATTTGCTCGA 
GACACAGGAA ACATTGGGCA GGAGCGTGTA GATACAGTTA ACCACATGGC AGATGACCTC 
ATCAATTCTG GACATTCAGA TGCTGCGACT ATTGCTGAGT GGAAAGATGG TCTCAATGAA 
GCCTGGGCTG ACCTCCTGGA GCTCATTGAC ACGAGAACAC AGATTCTTGC TGCCTCTTAC 
GAACTTCATA AGTTTTACCA TGATGCCAAG GAGATCTTTG GCCGCATCCA GGACAAACAC 
AAGAAACTCC CTGAGGAGCT TGGAAGAGAT CAGAACACCG TGGAGACTTT ACAGAGAATG 
CACACCACCT TTGAACATGA CATCCAAGCT CTGGGCACTC AGGTGAGGCA GCTGCAGGAG 
GATGCAGCCC GCCTCCAAGC AGCCTATGCA GGGGACAAGG CTGACGACAT CCAAAAGCGT 
GAGAACGAGG TCCTGGAAGC CTGGAAGTCC CTGCTGGATG CTTGTGAGGG TCGCAGGGTG 
CGGCTAGTAG ACACGGGAGA CAAGTTCCGC TTCTTCAGCA TGGTGCGTGA CCTCATGCTC 
TGGATGGAAG ATGTCATCCG GCAAATCGAG GCCCAGGAGA AACCCCGGGA TGTGTCATCT 
GTTGAACTGT TAATGAATAA TCATCAAGGC ATCAAAGCTG AAATCGATGC TCGTAATGAC 
AGTTTTACAG CTTGCATTGA ACTTGGGAAA GCCCTGCTGG CAAGGAAACA CTATGCTTCT 
GAGGAGATCA AGGAAAAGTT ACTGCAGTTG ACGGAGAAAA GAAAAGAAAT GATCGACAAG 
TGGGAAGACC GATGGGAGTG GTTAAGACTG ATTTTGGAGG TCCATCAGTT CTCAAGAGAT 
GCCAGTGTGG CTGAGGCTTG GCTGCTGGGA CAGGAGCCAT ACCTATCCAG CCGCGAAATC 
GGCCAGAGTG TGGATGAAGT GGAGAAGCTT ATTAAGCGCC ATGAGGCATT TGAAAAATCT 
GCAGCGACCT GGGATGAGAG ATTCTCTGCC CTGGAAAGGT TGACAACATT GGAGCTACTG 
GAAGTGCGCA GACAGCAAGA GGAAGAAGAA AGAAAGAGGC GACCACCGTC TCCGGAGCCA 
AGCGCGAAGG TTTCAGAGGA GGCTGAGTCC CAGCAATGGG ACACTTCAAA AGGAGACCAA 
GTTTCCCAGA ATGGTTTGCC GGCTGAGCAG GGATCTCCAC GGATGGCAGG AACCATGGAA 
ACAAGTGAAA TGGTCAACGG TGCTGCTGAG CAGAGGACAA GCTCCAAAGA ATCCAGTCCT 
GTTCCTTCTC CCACCTCGGA CCGCAAGGCC AAATCTGCAC TTCCAGCCCA GAGTGCTGCC 
ACCCTGCCAG CCAGGACCCT GGAGACGCCT GCGGCCCAGA TGGAAGGCTT CCTCAATCGG 
AAGCACGAGT GGGAGGCCCA CAATAAGAAA GCCTCAAGCA GGTCCTGGCA CAATGTTTAT 
TGTGTCATAA ATAACCAAGA AATGGGCTTC TACAAAGATG CCAAGAGTGC TGCTTCTGGC 
GTCCCCTACC ACAGTGAGGT CCCTGTGAGT TTGAAAGAGG CCATCTGCGA AGTGGCCCTT 
GATTACAAGA AGAAGAAGCA TGTGTTCAAG CTAAGACTAA GTGATGGCAA CGAGTACCTC 
TTCCAAGCCA AAGATGATGA TGAAATGAAC ACATGGATCC AGGCTATCAC CTCTGCCATC 
TCCTCTGACA AGCACGACAC GTCTGCCAGC ACCCAGAGTA CGCCAGCATC CAGCCGAGCG 
CAGACCTTAC CCACCAGCGT CGTCACCATC ACCAGCGAGT CCAGTCCTGG CAAGAGGGAG 
AAGGACAAAG AGAAAGACAA AGAGAAGCGG TTCAGCCTTT TCGGCAAGAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013148.1
CDS	126..7202
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTTSAQNKVLDGASVFLLMYSCPPRCWRIRWKRGRAQMRVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTSDRKAKSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASGVPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDDEMNTWIQAITSAISSDKHDTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	NM_001013130.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013130.1

ORF Insert Sequence:

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA CTAGCGCCCA AAACAAAGTT 
CTAGATGGAG CCTCTGTGTT CCTTCTGATG TACTCTTGCC CCCCCAGATG CTGGAGAATC 
AGATGGAAGA GGGGAAGAGC ACAGATGAGG GTGGACAGCA AACGCCTCAC TGTGCAGACC 
AAGTTCATGG AGCTTCTGGA GCCCTTGAAT GAAAGGAAGC ATAATCTGCT GGCTTCCAAG 
GAGATCCACC AGTTCAACAG GGATGTAGAG GACGAGATTC TGTGGGTTGG CGAGAGGATG 
CCTTTGGCAA CTTCCACAGA TCATGGCCAC AACCTTCAAA CTGTGCAGCT GTTAATAAAG 
AAAAACCAGA CCCTCCAGAA AGAAATCCAG GGACACCAGC CTCGTATTGA TGACATCTTT 
GAGAGGAGCC AAAACATCAT CACAGATAGC AGCAGCCTCA ATGCCGAGGC TATCAGACAG 
AGGCTCGCCG ATCTGAAGCA GCTGTGGGGG CTCCTCATTG AGGAAACTGA GAAACGCCAT 
AGACGGCTCG AGGAGGCACA CAAGGCCCAG CAATACTACT TTGATGCAGC TGAAGCAGAG 
GCATGGATGA GTGAACAGGA ACTGTACATG ATGTCTGAGG AAAAGGCCAA GGATGAGCAG 
AGTGCTGTCT CCATGTTGAA AAAGCACCAG ATTTTGGAAC AGGCTGTTGA GGACTATGCA 
GAGACAGTGC ACCAGCTCTC CAAGACCAGC CGGGCACTGG TGGCTGACAG CCATCCCGAA 
AGTGAGCGTA TTAGCATGCG GCAGTCAAAG GTCGACAAGC TGTATGCTGG CCTGAAGGAC 
CTTGCTGAGG AGAGGAGAGG GAAACTTGAT GAGAGGCACA GGCTATTCCA GCTCAACAGG 
GAGGTAGATG ACCTGGAACA GTGGATCGCC GAGAGGGAAG TGGTTGCAGG CTCCCATGAG 
TTGGGACAGG ACTATGAGCA TGTCACGATG TTACAAGAAC GGTTCCGAGA ATTTGCTCGA 
GACACAGGAA ACATTGGGCA GGAGCGTGTA GATACAGTTA ACCACATGGC AGATGACCTC 
ATCAATTCTG GACATTCAGA TGCTGCGACT ATTGCTGAGT GGAAAGATGG TCTCAATGAA 
GCCTGGGCTG ACCTCCTGGA GCTCATTGAC ACGAGAACAC AGATTCTTGC TGCCTCTTAC 
GAACTTCATA AGTTTTACCA TGATGCCAAG GAGATCTTTG GCCGCATCCA GGACAAACAC 
AAGAAACTCC CTGAGGAGCT TGGAAGAGAT CAGAACACCG TGGAGACTTT ACAGAGAATG 
CACACCACCT TTGAACATGA CATCCAAGCT CTGGGCACTC AGGTGAGGCA GCTGCAGGAG 
GATGCAGCCC GCCTCCAAGC AGCCTATGCA GGGGACAAGG CTGACGACAT CCAAAAGCGT 
GAGAACGAGG TCCTGGAAGC CTGGAAGTCC CTGCTGGATG CTTGTGAGGG TCGCAGGGTG 
CGGCTAGTAG ACACGGGAGA CAAGTTCCGC TTCTTCAGCA TGGTGCGTGA CCTCATGCTC 
TGGATGGAAG ATGTCATCCG GCAAATCGAG GCCCAGGAGA AACCCCGGGA TGTGTCATCT 
GTTGAACTGT TAATGAATAA TCATCAAGGC ATCAAAGCTG AAATCGATGC TCGTAATGAC 
AGTTTTACAG CTTGCATTGA ACTTGGGAAA GCCCTGCTGG CAAGGAAACA CTATGCTTCT 
GAGGAGATCA AGGAAAAGTT ACTGCAGTTG ACGGAGAAAA GAAAAGAAAT GATCGACAAG 
TGGGAAGACC GATGGGAGTG GTTAAGACTG ATTTTGGAGG TCCATCAGTT CTCAAGAGAT 
GCCAGTGTGG CTGAGGCTTG GCTGCTGGGA CAGGAGCCAT ACCTATCCAG CCGCGAAATC 
GGCCAGAGTG TGGATGAAGT GGAGAAGCTT ATTAAGCGCC ATGAGGCATT TGAAAAATCT 
GCAGCGACCT GGGATGAGAG ATTCTCTGCC CTGGAAAGGT TGACAACATT GGAGCTACTG 
GAAGTGCGCA GACAGCAAGA GGAAGAAGAA AGAAAGAGGC GACCACCGTC TCCGGAGCCA 
AGCGCGAAGG TTTCAGAGGA GGCTGAGTCC CAGCAATGGG ACACTTCAAA AGGAGACCAA 
GTTTCCCAGA ATGGTTTGCC GGCTGAGCAG GGATCTCCAC GGATGGCAGG AACCATGGAA 
ACAAGTGAAA TGGTCAACGG TGCTGCTGAG CAGAGGACAA GCTCCAAAGA ATCCAGTCCT 
GTTCCTTCTC CCACCTCGGA CCGCAAGGCC AAATCTGCAC TTCCAGCCCA GAGTGCTGCC 
ACCCTGCCAG CCAGGACCCT GGAGACGCCT GCGGCCCAGA TGGAAGGCTT CCTCAATCGG 
AAGCACGAGT GGGAGGCCCA CAATAAGAAA GCCTCAAGCA GGTCCTGGCA CAATGTTTAT 
TGTGTCATAA ATAACCAAGA AATGGGCTTC TACAAAGATG CCAAGAGTGC TGCTTCTGGC 
GTCCCCTACC ACAGTGAGGT CCCTGTGAGT TTGAAAGAGG CCATCTGCGA AGTGGCCCTT 
GATTACAAGA AGAAGAAGCA TGTGTTCAAG CTAAGACTAA GTGATGGCAA CGAGTACCTC 
TTCCAAGCCA AAGATGATGA TGAAATGAAC ACATGGATCC AGGCTATCAC CTCTGCCATC 
TCCTCTGACA AGCACGACAC GTCTGCCAGC ACCCAGAGTA CGCCAGCATC CAGCCGAGCG 
CAGACCTTAC CCACCAGCGT CGTCACCATC ACCAGCGAGT CCAGTCCTGG CAAGAGGGAG 
AAGGACAAAG AGAAAGACAA AGAGAAGCGG TTCAGCCTTT TCGGCAAGAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn1 ( XM_008770461.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768683.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA.

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CloneID	ORa46315
Clone ID Related Accession (Same CDS sequence)	XM_008770461.2 , XM_008770458.2 , XM_017599259.1
Accession Version	XM_008770461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG 
GCAGGAACCA TGGAAACAAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC 
AAAGAATCCA GTCCTGTTCC TTCTCCCACC TCGGACCGCA AGGCCAAATC TGCACTTCCA 
GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CGCCTGCGGC CCAGATGGAA 
GGCTTCCTCA ATCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC AAGCAGGTCC 
TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAAATGG GCTTCTACAA AGATGCCAAG 
AGTGCTGCTT CTGGCGTCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC 
TGCGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCATGTGT TCAAGCTAAG ACTAAGTGAT 
GGCAACGAGT ACCTCTTCCA AGCCAAAGAT GATGATGAAA TGAACACATG GATCCAGGCT 
ATCACCTCTG CCATCTCCTC TGACAAGCAC GACACGTCTG CCAGCACCCA GAGTACGCCA 
GCATCCAGCC GAGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT 
CCTGGCAAGA GGGAGAAGGA CAAAGAGAAA GACAAAGAGA AGCGGTTCAG CCTTTTCGGC 
AAGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768683.1
CDS	141..7232
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTSDRKAKSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASGVPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDDEMNTWIQAITSAISSDKHDTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_008770461.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770461.2

ORF Insert Sequence:

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG 
GCAGGAACCA TGGAAACAAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC 
AAAGAATCCA GTCCTGTTCC TTCTCCCACC TCGGACCGCA AGGCCAAATC TGCACTTCCA 
GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CGCCTGCGGC CCAGATGGAA 
GGCTTCCTCA ATCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC AAGCAGGTCC 
TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAAATGG GCTTCTACAA AGATGCCAAG 
AGTGCTGCTT CTGGCGTCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC 
TGCGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCATGTGT TCAAGCTAAG ACTAAGTGAT 
GGCAACGAGT ACCTCTTCCA AGCCAAAGAT GATGATGAAA TGAACACATG GATCCAGGCT 
ATCACCTCTG CCATCTCCTC TGACAAGCAC GACACGTCTG CCAGCACCCA GAGTACGCCA 
GCATCCAGCC GAGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT 
CCTGGCAAGA GGGAGAAGGA CAAAGAGAAA GACAAAGAGA AGCGGTTCAG CCTTTTCGGC 
AAGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn1 ( XM_008770458.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768680.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA.

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CloneID	ORa46315
Clone ID Related Accession (Same CDS sequence)	XM_008770461.2 , XM_008770458.2 , XM_017599259.1
Accession Version	XM_008770458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770458.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG 
GCAGGAACCA TGGAAACAAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC 
AAAGAATCCA GTCCTGTTCC TTCTCCCACC TCGGACCGCA AGGCCAAATC TGCACTTCCA 
GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CGCCTGCGGC CCAGATGGAA 
GGCTTCCTCA ATCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC AAGCAGGTCC 
TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAAATGG GCTTCTACAA AGATGCCAAG 
AGTGCTGCTT CTGGCGTCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC 
TGCGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCATGTGT TCAAGCTAAG ACTAAGTGAT 
GGCAACGAGT ACCTCTTCCA AGCCAAAGAT GATGATGAAA TGAACACATG GATCCAGGCT 
ATCACCTCTG CCATCTCCTC TGACAAGCAC GACACGTCTG CCAGCACCCA GAGTACGCCA 
GCATCCAGCC GAGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT 
CCTGGCAAGA GGGAGAAGGA CAAAGAGAAA GACAAAGAGA AGCGGTTCAG CCTTTTCGGC 
AAGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768680.1
CDS	1660..8751
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTSDRKAKSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASGVPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDDEMNTWIQAITSAISSDKHDTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_008770458.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770458.2

ORF Insert Sequence:

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG 
GCAGGAACCA TGGAAACAAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC 
AAAGAATCCA GTCCTGTTCC TTCTCCCACC TCGGACCGCA AGGCCAAATC TGCACTTCCA 
GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CGCCTGCGGC CCAGATGGAA 
GGCTTCCTCA ATCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC AAGCAGGTCC 
TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAAATGG GCTTCTACAA AGATGCCAAG 
AGTGCTGCTT CTGGCGTCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC 
TGCGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCATGTGT TCAAGCTAAG ACTAAGTGAT 
GGCAACGAGT ACCTCTTCCA AGCCAAAGAT GATGATGAAA TGAACACATG GATCCAGGCT 
ATCACCTCTG CCATCTCCTC TGACAAGCAC GACACGTCTG CCAGCACCCA GAGTACGCCA 
GCATCCAGCC GAGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT 
CCTGGCAAGA GGGAGAAGGA CAAAGAGAAA GACAAAGAGA AGCGGTTCAG CCTTTTCGGC 
AAGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn1 ( XM_017599259.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454748.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA.

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CloneID	ORa46315
Clone ID Related Accession (Same CDS sequence)	XM_008770461.2 , XM_008770458.2 , XM_017599259.1
Accession Version	XM_017599259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG 
GCAGGAACCA TGGAAACAAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC 
AAAGAATCCA GTCCTGTTCC TTCTCCCACC TCGGACCGCA AGGCCAAATC TGCACTTCCA 
GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CGCCTGCGGC CCAGATGGAA 
GGCTTCCTCA ATCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC AAGCAGGTCC 
TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAAATGG GCTTCTACAA AGATGCCAAG 
AGTGCTGCTT CTGGCGTCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC 
TGCGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCATGTGT TCAAGCTAAG ACTAAGTGAT 
GGCAACGAGT ACCTCTTCCA AGCCAAAGAT GATGATGAAA TGAACACATG GATCCAGGCT 
ATCACCTCTG CCATCTCCTC TGACAAGCAC GACACGTCTG CCAGCACCCA GAGTACGCCA 
GCATCCAGCC GAGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT 
CCTGGCAAGA GGGAGAAGGA CAAAGAGAAA GACAAAGAGA AGCGGTTCAG CCTTTTCGGC 
AAGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454748.1
CDS	228..7319
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTSDRKAKSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASGVPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDDEMNTWIQAITSAISSDKHDTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_017599259.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599259.1

ORF Insert Sequence:

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGATG 
GCAGGAACCA TGGAAACAAG TGAAATGGTC AACGGTGCTG CTGAGCAGAG GACAAGCTCC 
AAAGAATCCA GTCCTGTTCC TTCTCCCACC TCGGACCGCA AGGCCAAATC TGCACTTCCA 
GCCCAGAGTG CTGCCACCCT GCCAGCCAGG ACCCTGGAGA CGCCTGCGGC CCAGATGGAA 
GGCTTCCTCA ATCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC AAGCAGGTCC 
TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAAATGG GCTTCTACAA AGATGCCAAG 
AGTGCTGCTT CTGGCGTCCC CTACCACAGT GAGGTCCCTG TGAGTTTGAA AGAGGCCATC 
TGCGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCATGTGT TCAAGCTAAG ACTAAGTGAT 
GGCAACGAGT ACCTCTTCCA AGCCAAAGAT GATGATGAAA TGAACACATG GATCCAGGCT 
ATCACCTCTG CCATCTCCTC TGACAAGCAC GACACGTCTG CCAGCACCCA GAGTACGCCA 
GCATCCAGCC GAGCGCAGAC CTTACCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT 
CCTGGCAAGA GGGAGAAGGA CAAAGAGAAA GACAAAGAGA AGCGGTTCAG CCTTTTCGGC 
AAGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn1 ( XM_008770460.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768682.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X4, mRNA.

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CloneID	ORa46316
Clone ID Related Accession (Same CDS sequence)	XM_008770460.2
Accession Version	XM_008770460.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770460.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
GTGCCTTACA ACTACAATCA ACTGGAAGGG AGATTCAAAC AGCTCCAAGA TGAGCGTGAA 
GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG AGTGTCCTGC 
CGAATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA ACTACTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGCAT CCACTGTCTG 
GAGAATGTCG ACAAGGCTCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT CGAGAACATG 
GGCTCCCATG ATATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATT 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG 
TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCTAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCGAATGC ACACTACAAT 
CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TTACTAAACT GTTAGACCCT 
GAAGATATCA GCGTGGACCA CCCTGATGAG AAGTCCATCA TCACGTATGT GGTCACTTAC 
TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGGAAGGTG 
CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ATGAGTCGCT TGCTTCTGAC 
CTTCTGGAGT GGATTGAACA AACCATCATC ATTCTAAACA ACCGCAAATT TGCTAATTCA 
CTGGTTGGGG TCCAGCAGCA GCTTCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA 
CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTGCTTT TCACAATTCA GAGCAAGATG 
AGAGCTAATA ATCAGAAGGT CTACATGCCC CGAGAAGGGA AGCTCATCTC TGATATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG 
CTCATACGGC AGGAAAAACT GGAACAACTT GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGAGAGACAT GGCTGAGCGA AAACCAGCGT CTTGTCTCTC AGGACAACTT TGGATTTGAC 
CTTCCTGCAG TTGAGGCCGC CACCAAAAAA CATGAGGCCA TCGAGACTGA CATTGCGGCG 
TATGAAGAGC GAGTGCAGGC TGTGGTGGCT GTGGCCAGGG AGCTTGAAGC AGAGAGCTAT 
CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGCCTCTG GGAGTACTTG 
CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGACT GCAAAAGATA 
TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT 
CAGGACTATG GCAAACACTT GCTCGGTGTT GAAGACTTGT TACAGAAGCA TGCCCTGGTT 
GAAGCAGACA TTGCAATCCA AGCAGAACGT GTGAGAGGTG TGAATGCCTC TGCCCAGAAG 
TTCGCAACAG ATGGGGAAGG CTACAAGCCA TGCGACCCCC AGGTAATCCG AGACCGTGTG 
GCCCACATGG AATTCTGCTA TCAAGAACTC TGTCAGCTGG CAGCTGAGCG TAGGGCTCGC 
CTGGAAGAGT CTCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAGGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTATG GGAAAGACCT GACTAGTGTC 
ATGCGCCTGC TGAGCAAGCA CAGGGCTTTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT 
TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCAGAA 
AAGATCCGTG AGAGAATCAG TTACATTCGA GAGCAGTGGG CCAACCTGGA GCAGCTCTCA 
GCCATTAGGA AGAAGCGCCT AGAGGAGGCC TCATTGCTGC ACCAGTTCCA GGCTGATGCT 
GATGACATCG ACGCTTGGAT GTTAGATATA CTCAAGATTG TGTCCAGCAA CGATGTGGGC 
CATGATGAGT ATTCCACACA GTCTCTGGTC AAGAAGCATA AAGATGTGGC AGAAGAGATC 
TCCAACTACA GACCCACTAT TGACACACTG CATGAGCAAG CCGGTGCTCT TCCTCAAGCA 
CACGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG TTACAAGGAG 
GTGGCAGAGT TAACACGGCT AAGGAAGCAG GCACTGCAGG ACACCCTGGC TCTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG 
CAGCTGATGC ACAGTGGCCA TCCCAGCGAA AAGGAAATCA GAGCTCAGCA GGACAAACTC 
AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AGAAGGATGC TCTTCTGTCC 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG 
AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAACGA GACTTGGTAG CCATCGAGGC GAAGCTGAGT 
GACCTGCAGA AGGAAGCCGA GAAGCTTGAG TCCGAGCACC CTGACCAGGC TCAAGCCATC 
CTGTCTCGAC TGGCTGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAGAAC 
CGAGAAGCCT CGCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CTTGGATGAC 
TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATTGCCT CAGAGGACAT GCCCAATACC 
CTGACTGAGG CAGAGAAGCT TCTCACACAG CATGAGAACA TCAAAAACGA GATTGACAAT 
TACGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACGCA GGGACAGACT 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG TTGCAGGCCT TGGACACAGG CTGGAACGAG 
CTCCACAAAA TGTGGGAGAA CAGGCAAAAT CTCCTCTCCC AGTCCCATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAACA GGCTGAAGCT TTCCTTAATA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAGAA GCAGGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA TATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGGC ACAGAAAGAA TCGAGAAGCA GCCAGTGAAC TTTTGATGAG GTTAAAGGAC 
AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA 
AAGATGCTTA CAGCTCAGGA CATGTCTTAT GATGAAGCCA GAAACCTGCA CAGTAAATGG 
TTAAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAGGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC 
CAGAGGCTCT TCGATGCGAA CAAGGCTGAG CTCTTCACGC AGAGCTGTGC AGACCTCGAC 
AAATGGCTGC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTCACC 
AGTGTCAACA TTCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGAGGAAG 
AAGGAGATCG AGGAACTGCA AAGCCAAGCC CAGGCACTGA GTCAGGAGGG GAAGAGCACA 
GATGAGGTGG ACAGCAAACG CCTCACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC 
TTGAATGAAA GGAAGCATAA TCTGCTGGCT TCCAAGGAGA TCCACCAGTT CAACAGGGAT 
GTAGAGGACG AGATTCTGTG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCATCACA 
GATAGCAGCA GCCTCAATGC CGAGGCTATC AGACAGAGGC TCGCCGATCT GAAGCAGCTG 
TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTCGAGGA GGCACACAAG 
GCCCAGCAAT ACTACTTTGA TGCAGCTGAA GCAGAGGCAT GGATGAGTGA ACAGGAACTG 
TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAAAAG 
CACCAGATTT TGGAACAGGC TGTTGAGGAC TATGCAGAGA CAGTGCACCA GCTCTCCAAG 
ACCAGCCGGG CACTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG 
TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGGAAA 
CTTGATGAGA GGCACAGGCT ATTCCAGCTC AACAGGGAGG TAGATGACCT GGAACAGTGG 
ATCGCCGAGA GGGAAGTGGT TGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG 
CGTGTAGATA CAGTTAACCA CATGGCAGAT GACCTCATCA ATTCTGGACA TTCAGATGCT 
GCGACTATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATTGACACGA GAACACAGAT TCTTGCTGCC TCTTACGAAC TTCATAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGCCG CATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA 
AGAGATCAGA ACACCGTGGA GACTTTACAG AGAATGCACA CCACCTTTGA ACATGACATC 
CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAAGCAGCC 
TATGCAGGGG ACAAGGCTGA CGACATCCAA AAGCGTGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TAGTAGACAC GGGAGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAA 
ATCGAGGCCC AGGAGAAACC CCGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT CGATGCTCGT AATGACAGTT TTACAGCTTG CATTGAACTT 
GGGAAAGCCC TGCTGGCAAG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG 
CAGTTGACGG AGAAAAGAAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAGTGGTTA 
AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGAGATGCCA GTGTGGCTGA GGCTTGGCTG 
CTGGGACAGG AGCCATACCT ATCCAGCCGC GAAATCGGCC AGAGTGTGGA TGAAGTGGAG 
AAGCTTATTA AGCGCCATGA GGCATTTGAA AAATCTGCAG CGACCTGGGA TGAGAGATTC 
TCTGCCCTGG AAAGGTTGAC AACATTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAAGAAAGAA AGAGGCGACC ACCGTCTCCG GAGCCAAGCG CGAAGGTTTC AGAGGAGGCT 
GAGTCCCAGC AATGGGACAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT 
GAGCAGGGAT CTCCACGGAT GGCAGGAACC ATGGAAACAA GTGAAATGGT CAACGGTGCT 
GCTGAGCAGA GGACAAGCTC CAAAGAATCC AGTCCTGTTC CTTCTCCCAC CTCGGACCGC 
AAGGCCAAAT CTGCACTTCC AGCCCAGAGT GCTGCCACCC TGCCAGCCAG GACCCTGGAG 
ACGCCTGCGG CCCAGATGGA AGGCTTCCTC AATCGGAAGC ACGAGTGGGA GGCCCACAAT 
AAGAAAGCCT CAAGCAGGTC CTGGCACAAT GTTTATTGTG TCATAAATAA CCAAGAAATG 
GGCTTCTACA AAGATGCCAA GAGTGCTGCT TCTGGCGTCC CCTACCACAG TGAGGTCCCT 
GTGAGTTTGA AAGAGGCCAT CTGCGAAGTG GCCCTTGATT ACAAGAAGAA GAAGCATGTG 
TTCAAGCTAA GACTAAGTGA TGGCAACGAG TACCTCTTCC AAGCCAAAGA TGATGATGAA 
ATGAACACAT GGATCCAGGC TATCACCTCT GCCATCTCCT CTGACAAGCA CGACACGTCT 
GCCAGCACCC AGAGTACGCC AGCATCCAGC CGAGCGCAGA CCTTACCCAC CAGCGTCGTC 
ACCATCACCA GCGAGTCCAG TCCTGGCAAG AGGGAGAAGG ACAAAGAGAA AGACAAAGAG 
AAGCGGTTCA GCCTTTTCGG CAAGAAGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768682.1
CDS	440..7492
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPTSDRKAKSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKSAASGVPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDDEMNTWIQAITSAISSDKHDTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_008770460.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770460.2

ORF Insert Sequence:

ATGGAATTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
GTGCCTTACA ACTACAATCA ACTGGAAGGG AGATTCAAAC AGCTCCAAGA TGAGCGTGAA 
GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG AGTGTCCTGC 
CGAATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA ACTACTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGCAT CCACTGTCTG 
GAGAATGTCG ACAAGGCTCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT CGAGAACATG 
GGCTCCCATG ATATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATT 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG 
TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCTAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCGAATGC ACACTACAAT 
CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TTACTAAACT GTTAGACCCT 
GAAGATATCA GCGTGGACCA CCCTGATGAG AAGTCCATCA TCACGTATGT GGTCACTTAC 
TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGGAAGGTG 
CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ATGAGTCGCT TGCTTCTGAC 
CTTCTGGAGT GGATTGAACA AACCATCATC ATTCTAAACA ACCGCAAATT TGCTAATTCA 
CTGGTTGGGG TCCAGCAGCA GCTTCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA 
CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTGCTTT TCACAATTCA GAGCAAGATG 
AGAGCTAATA ATCAGAAGGT CTACATGCCC CGAGAAGGGA AGCTCATCTC TGATATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG 
CTCATACGGC AGGAAAAACT GGAACAACTT GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGAGAGACAT GGCTGAGCGA AAACCAGCGT CTTGTCTCTC AGGACAACTT TGGATTTGAC 
CTTCCTGCAG TTGAGGCCGC CACCAAAAAA CATGAGGCCA TCGAGACTGA CATTGCGGCG 
TATGAAGAGC GAGTGCAGGC TGTGGTGGCT GTGGCCAGGG AGCTTGAAGC AGAGAGCTAT 
CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGCCTCTG GGAGTACTTG 
CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGACT GCAAAAGATA 
TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT 
CAGGACTATG GCAAACACTT GCTCGGTGTT GAAGACTTGT TACAGAAGCA TGCCCTGGTT 
GAAGCAGACA TTGCAATCCA AGCAGAACGT GTGAGAGGTG TGAATGCCTC TGCCCAGAAG 
TTCGCAACAG ATGGGGAAGG CTACAAGCCA TGCGACCCCC AGGTAATCCG AGACCGTGTG 
GCCCACATGG AATTCTGCTA TCAAGAACTC TGTCAGCTGG CAGCTGAGCG TAGGGCTCGC 
CTGGAAGAGT CTCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAGGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTATG GGAAAGACCT GACTAGTGTC 
ATGCGCCTGC TGAGCAAGCA CAGGGCTTTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT 
TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCAGAA 
AAGATCCGTG AGAGAATCAG TTACATTCGA GAGCAGTGGG CCAACCTGGA GCAGCTCTCA 
GCCATTAGGA AGAAGCGCCT AGAGGAGGCC TCATTGCTGC ACCAGTTCCA GGCTGATGCT 
GATGACATCG ACGCTTGGAT GTTAGATATA CTCAAGATTG TGTCCAGCAA CGATGTGGGC 
CATGATGAGT ATTCCACACA GTCTCTGGTC AAGAAGCATA AAGATGTGGC AGAAGAGATC 
TCCAACTACA GACCCACTAT TGACACACTG CATGAGCAAG CCGGTGCTCT TCCTCAAGCA 
CACGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG TTACAAGGAG 
GTGGCAGAGT TAACACGGCT AAGGAAGCAG GCACTGCAGG ACACCCTGGC TCTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG 
CAGCTGATGC ACAGTGGCCA TCCCAGCGAA AAGGAAATCA GAGCTCAGCA GGACAAACTC 
AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AGAAGGATGC TCTTCTGTCC 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG 
AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAACGA GACTTGGTAG CCATCGAGGC GAAGCTGAGT 
GACCTGCAGA AGGAAGCCGA GAAGCTTGAG TCCGAGCACC CTGACCAGGC TCAAGCCATC 
CTGTCTCGAC TGGCTGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAGAAC 
CGAGAAGCCT CGCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CTTGGATGAC 
TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATTGCCT CAGAGGACAT GCCCAATACC 
CTGACTGAGG CAGAGAAGCT TCTCACACAG CATGAGAACA TCAAAAACGA GATTGACAAT 
TACGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACGCA GGGACAGACT 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG TTGCAGGCCT TGGACACAGG CTGGAACGAG 
CTCCACAAAA TGTGGGAGAA CAGGCAAAAT CTCCTCTCCC AGTCCCATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAACA GGCTGAAGCT TTCCTTAATA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAGAA GCAGGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA TATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGGC ACAGAAAGAA TCGAGAAGCA GCCAGTGAAC TTTTGATGAG GTTAAAGGAC 
AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA 
AAGATGCTTA CAGCTCAGGA CATGTCTTAT GATGAAGCCA GAAACCTGCA CAGTAAATGG 
TTAAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAGGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC 
CAGAGGCTCT TCGATGCGAA CAAGGCTGAG CTCTTCACGC AGAGCTGTGC AGACCTCGAC 
AAATGGCTGC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTCACC 
AGTGTCAACA TTCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGAGGAAG 
AAGGAGATCG AGGAACTGCA AAGCCAAGCC CAGGCACTGA GTCAGGAGGG GAAGAGCACA 
GATGAGGTGG ACAGCAAACG CCTCACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC 
TTGAATGAAA GGAAGCATAA TCTGCTGGCT TCCAAGGAGA TCCACCAGTT CAACAGGGAT 
GTAGAGGACG AGATTCTGTG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCATCACA 
GATAGCAGCA GCCTCAATGC CGAGGCTATC AGACAGAGGC TCGCCGATCT GAAGCAGCTG 
TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTCGAGGA GGCACACAAG 
GCCCAGCAAT ACTACTTTGA TGCAGCTGAA GCAGAGGCAT GGATGAGTGA ACAGGAACTG 
TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAAAAG 
CACCAGATTT TGGAACAGGC TGTTGAGGAC TATGCAGAGA CAGTGCACCA GCTCTCCAAG 
ACCAGCCGGG CACTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG 
TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGGAAA 
CTTGATGAGA GGCACAGGCT ATTCCAGCTC AACAGGGAGG TAGATGACCT GGAACAGTGG 
ATCGCCGAGA GGGAAGTGGT TGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG 
CGTGTAGATA CAGTTAACCA CATGGCAGAT GACCTCATCA ATTCTGGACA TTCAGATGCT 
GCGACTATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATTGACACGA GAACACAGAT TCTTGCTGCC TCTTACGAAC TTCATAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGCCG CATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA 
AGAGATCAGA ACACCGTGGA GACTTTACAG AGAATGCACA CCACCTTTGA ACATGACATC 
CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAAGCAGCC 
TATGCAGGGG ACAAGGCTGA CGACATCCAA AAGCGTGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TAGTAGACAC GGGAGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAA 
ATCGAGGCCC AGGAGAAACC CCGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT CGATGCTCGT AATGACAGTT TTACAGCTTG CATTGAACTT 
GGGAAAGCCC TGCTGGCAAG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG 
CAGTTGACGG AGAAAAGAAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAGTGGTTA 
AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGAGATGCCA GTGTGGCTGA GGCTTGGCTG 
CTGGGACAGG AGCCATACCT ATCCAGCCGC GAAATCGGCC AGAGTGTGGA TGAAGTGGAG 
AAGCTTATTA AGCGCCATGA GGCATTTGAA AAATCTGCAG CGACCTGGGA TGAGAGATTC 
TCTGCCCTGG AAAGGTTGAC AACATTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAAGAAAGAA AGAGGCGACC ACCGTCTCCG GAGCCAAGCG CGAAGGTTTC AGAGGAGGCT 
GAGTCCCAGC AATGGGACAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT 
GAGCAGGGAT CTCCACGGAT GGCAGGAACC ATGGAAACAA GTGAAATGGT CAACGGTGCT 
GCTGAGCAGA GGACAAGCTC CAAAGAATCC AGTCCTGTTC CTTCTCCCAC CTCGGACCGC 
AAGGCCAAAT CTGCACTTCC AGCCCAGAGT GCTGCCACCC TGCCAGCCAG GACCCTGGAG 
ACGCCTGCGG CCCAGATGGA AGGCTTCCTC AATCGGAAGC ACGAGTGGGA GGCCCACAAT 
AAGAAAGCCT CAAGCAGGTC CTGGCACAAT GTTTATTGTG TCATAAATAA CCAAGAAATG 
GGCTTCTACA AAGATGCCAA GAGTGCTGCT TCTGGCGTCC CCTACCACAG TGAGGTCCCT 
GTGAGTTTGA AAGAGGCCAT CTGCGAAGTG GCCCTTGATT ACAAGAAGAA GAAGCATGTG 
TTCAAGCTAA GACTAAGTGA TGGCAACGAG TACCTCTTCC AAGCCAAAGA TGATGATGAA 
ATGAACACAT GGATCCAGGC TATCACCTCT GCCATCTCCT CTGACAAGCA CGACACGTCT 
GCCAGCACCC AGAGTACGCC AGCATCCAGC CGAGCGCAGA CCTTACCCAC CAGCGTCGTC 
ACCATCACCA GCGAGTCCAG TCCTGGCAAG AGGGAGAAGG ACAAAGAGAA AGACAAAGAG 
AAGCGGTTCA GCCTTTTCGG CAAGAAGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn1 ( XM_008770462.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768684.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X5, mRNA.

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CloneID	ORa46317
Clone ID Related Accession (Same CDS sequence)	XM_008770462.2
Accession Version	XM_008770462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
GTGCCTTACA ACTACAATCA ACTGGAAGGG AGATTCAAAC AGCTCCAAGA TGAGCGTGAA 
GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG AGTGTCCTGC 
CGAATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA ACTACTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGCAT CCACTGTCTG 
GAGAATGTCG ACAAGGCTCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT CGAGAACATG 
GGCTCCCATG ATATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATT 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG 
TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCTAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCGAATGC ACACTACAAT 
CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TTACTAAACT GTTAGACCCT 
GAAGATATCA GCGTGGACCA CCCTGATGAG AAGTCCATCA TCACGTATGT GGTCACTTAC 
TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGGAAGGTG 
CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ATGAGTCGCT TGCTTCTGAC 
CTTCTGGAGT GGATTGAACA AACCATCATC ATTCTAAACA ACCGCAAATT TGCTAATTCA 
CTGGTTGGGG TCCAGCAGCA GCTTCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA 
CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTGCTTT TCACAATTCA GAGCAAGATG 
AGAGCTAATA ATCAGAAGGT CTACATGCCC CGAGAAGGGA AGCTCATCTC TGATATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG 
CTCATACGGC AGGAAAAACT GGAACAACTT GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGAGAGACAT GGCTGAGCGA AAACCAGCGT CTTGTCTCTC AGGACAACTT TGGATTTGAC 
CTTCCTGCAG TTGAGGCCGC CACCAAAAAA CATGAGGCCA TCGAGACTGA CATTGCGGCG 
TATGAAGAGC GAGTGCAGGC TGTGGTGGCT GTGGCCAGGG AGCTTGAAGC AGAGAGCTAT 
CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGCCTCTG GGAGTACTTG 
CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGACT GCAAAAGATA 
TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT 
CAGGACTATG GCAAACACTT GCTCGGTGTT GAAGACTTGT TACAGAAGCA TGCCCTGGTT 
GAAGCAGACA TTGCAATCCA AGCAGAACGT GTGAGAGGTG TGAATGCCTC TGCCCAGAAG 
TTCGCAACAG ATGGGGAAGG CTACAAGCCA TGCGACCCCC AGGTAATCCG AGACCGTGTG 
GCCCACATGG AATTCTGCTA TCAAGAACTC TGTCAGCTGG CAGCTGAGCG TAGGGCTCGC 
CTGGAAGAGT CTCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAGGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTATG GGAAAGACCT GACTAGTGTC 
ATGCGCCTGC TGAGCAAGCA CAGGGCTTTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT 
TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCAGAA 
AAGATCCGTG AGAGAATCAG TTACATTCGA GAGCAGTGGG CCAACCTGGA GCAGCTCTCA 
GCCATTAGGA AGAAGCGCCT AGAGGAGGCC TCATTGCTGC ACCAGTTCCA GGCTGATGCT 
GATGACATCG ACGCTTGGAT GTTAGATATA CTCAAGATTG TGTCCAGCAA CGATGTGGGC 
CATGATGAGT ATTCCACACA GTCTCTGGTC AAGAAGCATA AAGATGTGGC AGAAGAGATC 
TCCAACTACA GACCCACTAT TGACACACTG CATGAGCAAG CCGGTGCTCT TCCTCAAGCA 
CACGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG TTACAAGGAG 
GTGGCAGAGT TAACACGGCT AAGGAAGCAG GCACTGCAGG ACACCCTGGC TCTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG 
CAGCTGATGC ACAGTGGCCA TCCCAGCGAA AAGGAAATCA GAGCTCAGCA GGACAAACTC 
AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AGAAGGATGC TCTTCTGTCC 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG 
AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAACGA GACTTGGTAG CCATCGAGGC GAAGCTGAGT 
GACCTGCAGA AGGAAGCCGA GAAGCTTGAG TCCGAGCACC CTGACCAGGC TCAAGCCATC 
CTGTCTCGAC TGGCTGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAGAAC 
CGAGAAGCCT CGCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CTTGGATGAC 
TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATTGCCT CAGAGGACAT GCCCAATACC 
CTGACTGAGG CAGAGAAGCT TCTCACACAG CATGAGAACA TCAAAAACGA GATTGACAAT 
TACGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACGCA GGGACAGACT 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG TTGCAGGCCT TGGACACAGG CTGGAACGAG 
CTCCACAAAA TGTGGGAGAA CAGGCAAAAT CTCCTCTCCC AGTCCCATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAACA GGCTGAAGCT TTCCTTAATA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAGAA GCAGGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA TATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGGC ACAGAAAGAA TCGAGAAGCA GCCAGTGAAC TTTTGATGAG GTTAAAGGAC 
AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA 
AAGATGCTTA CAGCTCAGGA CATGTCTTAT GATGAAGCCA GAAACCTGCA CAGTAAATGG 
TTAAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAGGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC 
CAGAGGCTCT TCGATGCGAA CAAGGCTGAG CTCTTCACGC AGAGCTGTGC AGACCTCGAC 
AAATGGCTGC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTCACC 
AGTGTCAACA TTCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGAGGAAG 
AAGGAGATCG AGGAACTGCA AAGCCAAGCC CAGGCACTGA GTCAGGAGGG GAAGAGCACA 
GATGAGGTGG ACAGCAAACG CCTCACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC 
TTGAATGAAA GGAAGCATAA TCTGCTGGCT TCCAAGGAGA TCCACCAGTT CAACAGGGAT 
GTAGAGGACG AGATTCTGTG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCATCACA 
GATAGCAGCA GCCTCAATGC CGAGGCTATC AGACAGAGGC TCGCCGATCT GAAGCAGCTG 
TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTCGAGGA GGCACACAAG 
GCCCAGCAAT ACTACTTTGA TGCAGCTGAA GCAGAGGCAT GGATGAGTGA ACAGGAACTG 
TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAAAAG 
CACCAGATTT TGGAACAGGC TGTTGAGGAC TATGCAGAGA CAGTGCACCA GCTCTCCAAG 
ACCAGCCGGG CACTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG 
TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGGAAA 
CTTGATGAGA GGCACAGGCT ATTCCAGCTC AACAGGGAGG TAGATGACCT GGAACAGTGG 
ATCGCCGAGA GGGAAGTGGT TGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG 
CGTGTAGATA CAGTTAACCA CATGGCAGAT GACCTCATCA ATTCTGGACA TTCAGATGCT 
GCGACTATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATTGACACGA GAACACAGAT TCTTGCTGCC TCTTACGAAC TTCATAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGCCG CATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA 
AGAGATCAGA ACACCGTGGA GACTTTACAG AGAATGCACA CCACCTTTGA ACATGACATC 
CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAAGCAGCC 
TATGCAGGGG ACAAGGCTGA CGACATCCAA AAGCGTGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TAGTAGACAC GGGAGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAA 
ATCGAGGCCC AGGAGAAACC CCGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT CGATGCTCGT AATGACAGTT TTACAGCTTG CATTGAACTT 
GGGAAAGCCC TGCTGGCAAG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG 
CAGTTGACGG AGAAAAGAAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAGTGGTTA 
AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGAGATGCCA GTGTGGCTGA GGCTTGGCTG 
CTGGGACAGG AGCCATACCT ATCCAGCCGC GAAATCGGCC AGAGTGTGGA TGAAGTGGAG 
AAGCTTATTA AGCGCCATGA GGCATTTGAA AAATCTGCAG CGACCTGGGA TGAGAGATTC 
TCTGCCCTGG AAAGGTTGAC AACATTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAAGAAAGAA AGAGGCGACC ACCGTCTCCG GAGCCAAGCG CGAAGGTTTC AGAGGAGGCT 
GAGTCCCAGC AATGGGACAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT 
GAGCAGGGAT CTCCACGGGT TAGTTACCGC TCTCAAACGT ACCAAAACTA CAAAAACTTT 
AATAGCAGAC GGACAGCCAG TGACCATTCG TGGTCTGGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768684.1
CDS	1..6465
Translation	MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDHSWSGL

Target ORF information:

RefSeq Version	XM_008770462.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770462.2

ORF Insert Sequence:

ATGGAATTGC AGAGGACATC CAGCATTTCA GGGCCGCTGT CGCCGGCCTA CACCGGGCAG 
GTGCCTTACA ACTACAATCA ACTGGAAGGG AGATTCAAAC AGCTCCAAGA TGAGCGTGAA 
GCTGTACAGA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG AGTGTCCTGC 
CGAATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA ACTACTGGAG 
GTCCTCTCTG GAGAGAGGCT GCCTAAACCC ACTAAGGGAC GGATGCGCAT CCACTGTCTG 
GAGAATGTCG ACAAGGCTCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT CGAGAACATG 
GGCTCCCATG ATATTGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATT 
ATCCTGCGCT TCCAGATCCA GGATATCAGT GTGGAGACTG AAGATAACAA AGAGAAAAAG 
TCTGCTAAGG ATGCATTGCT GCTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCTAATGTC 
AACATTCACA ATTTCACCAC TAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC 
AAACACCGGC CTGACCTGAT AGATTTTGAT AAACTGAAGA AATCGAATGC ACACTACAAT 
CTGCAGAATG CATTTAACCT GGCAGAGCAG CACCTTGGCC TTACTAAACT GTTAGACCCT 
GAAGATATCA GCGTGGACCA CCCTGATGAG AAGTCCATCA TCACGTATGT GGTCACTTAC 
TACCACTACT TCTCCAAGAT GAAGGCCTTG GCTGTCGAAG GAAAGCGCAT TGGGAAGGTG 
CTTGATAATG CTATAGAAAC AGAGAAAATG ATTGAGAAGT ATGAGTCGCT TGCTTCTGAC 
CTTCTGGAGT GGATTGAACA AACCATCATC ATTCTAAACA ACCGCAAATT TGCTAATTCA 
CTGGTTGGGG TCCAGCAGCA GCTTCAGGCA TTCAACACGT ACCGCACAGT GGAGAAACCA 
CCTAAGTTTA CTGAGAAGGG GAATTTGGAG GTGCTGCTTT TCACAATTCA GAGCAAGATG 
AGAGCTAATA ATCAGAAGGT CTACATGCCC CGAGAAGGGA AGCTCATCTC TGATATCAAC 
AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CATGAGAGAG AACTGGCTCT GCGGAATGAG 
CTCATACGGC AGGAAAAACT GGAACAACTT GCCCGCAGAT TCGATCGCAA GGCAGCTATG 
AGAGAGACAT GGCTGAGCGA AAACCAGCGT CTTGTCTCTC AGGACAACTT TGGATTTGAC 
CTTCCTGCAG TTGAGGCCGC CACCAAAAAA CATGAGGCCA TCGAGACTGA CATTGCGGCG 
TATGAAGAGC GAGTGCAGGC TGTGGTGGCT GTGGCCAGGG AGCTTGAAGC AGAGAGCTAT 
CATGACATCA AGCGCATCAC AGCGAGGAAG GACAATGTCA TCCGCCTCTG GGAGTACTTG 
CTGGAACTGC TCAGGGCCAG GAGGCAGCGT CTTGAGATGA ACCTGGGACT GCAAAAGATA 
TTCCAGGAAA TGCTTTATAT TATGGACTGG ATGGATGAAA TGAAGGTGCT ATTGCTGTCT 
CAGGACTATG GCAAACACTT GCTCGGTGTT GAAGACTTGT TACAGAAGCA TGCCCTGGTT 
GAAGCAGACA TTGCAATCCA AGCAGAACGT GTGAGAGGTG TGAATGCCTC TGCCCAGAAG 
TTCGCAACAG ATGGGGAAGG CTACAAGCCA TGCGACCCCC AGGTAATCCG AGACCGTGTG 
GCCCACATGG AATTCTGCTA TCAAGAACTC TGTCAGCTGG CAGCTGAGCG TAGGGCTCGC 
CTGGAAGAGT CTCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAGGA GGAAGGCTGG 
ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTATG GGAAAGACCT GACTAGTGTC 
ATGCGCCTGC TGAGCAAGCA CAGGGCTTTT GAGGATGAGA TGAGTGGCCG TAGTGGCCAT 
TTTGAGCAGG CCATTAAAGA AGGTGAAGAC ATGATTGCAG AGGAACACTT TGGATCAGAA 
AAGATCCGTG AGAGAATCAG TTACATTCGA GAGCAGTGGG CCAACCTGGA GCAGCTCTCA 
GCCATTAGGA AGAAGCGCCT AGAGGAGGCC TCATTGCTGC ACCAGTTCCA GGCTGATGCT 
GATGACATCG ACGCTTGGAT GTTAGATATA CTCAAGATTG TGTCCAGCAA CGATGTGGGC 
CATGATGAGT ATTCCACACA GTCTCTGGTC AAGAAGCATA AAGATGTGGC AGAAGAGATC 
TCCAACTACA GACCCACTAT TGACACACTG CATGAGCAAG CCGGTGCTCT TCCTCAAGCA 
CACGCAGAGT CTCCAGATGT GAAGGGCCGG CTGGCAGGAA TTGAGGAGCG TTACAAGGAG 
GTGGCAGAGT TAACACGGCT AAGGAAGCAG GCACTGCAGG ACACCCTGGC TCTGTACAAG 
ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AACATGCAGA TCCCAGAGAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG ATTTGAGAGC 
CTAGAACCAG AAATGAACAA CCAGGCTTCC CGGGTTGCTG TGGTGAACCA GATTGCACGG 
CAGCTGATGC ACAGTGGCCA TCCCAGCGAA AAGGAAATCA GAGCTCAGCA GGACAAACTC 
AACACGAGGT GGAGTCAGTT CAGAGAACTG GTGGACAGGA AGAAGGATGC TCTTCTGTCC 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAATGAAA CCAAATCCTG GATCCGGGAG 
AAGACCAAGG TCATCGAGTC TACCCAAGAC CTTGGCAATG ACCTGGCAGG TGTCATGGCC 
CTGCAGCGCA AGCTGACCGG CATGGAACGA GACTTGGTAG CCATCGAGGC GAAGCTGAGT 
GACCTGCAGA AGGAAGCCGA GAAGCTTGAG TCCGAGCACC CTGACCAGGC TCAAGCCATC 
CTGTCTCGAC TGGCTGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACAAC CCTGAAGAAC 
CGAGAAGCCT CGCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CTTGGATGAC 
TTCCAGTCTT GGCTCTCCAG GACCCAGACT GCTATTGCCT CAGAGGACAT GCCCAATACC 
CTGACTGAGG CAGAGAAGCT TCTCACACAG CATGAGAACA TCAAAAACGA GATTGACAAT 
TACGAGGAAG ACTACCAGAA GATGCGGGAC ATGGGCGAGA TGGTCACGCA GGGACAGACT 
GATGCCCAGT ACATGTTTCT GCGGCAGCGG TTGCAGGCCT TGGACACAGG CTGGAACGAG 
CTCCACAAAA TGTGGGAGAA CAGGCAAAAT CTCCTCTCCC AGTCCCATGC CTACCAGCAG 
TTCCTTAGGG ACACGAAACA GGCTGAAGCT TTCCTTAATA ACCAGGAGTA TGTTTTGGCT 
CATACTGAAA TGCCCACCAC CCTGGAAGGA GCTGAAGCAG CCATTAAGAA GCAGGAGGAC 
TTCATGACCA CCATGGATGC CAATGAGGAG AAGATCAATG CTGTTGTGGA GACTGGCCGG 
AGGCTGGTGA GCGATGGGAA TATCAACTCC GACCGCATCC AGGAGAAGGT GGACTCTATT 
GATGACAGGC ACAGAAAGAA TCGAGAAGCA GCCAGTGAAC TTTTGATGAG GTTAAAGGAC 
AACCGTGATC TACAGAAGTT CCTGCAAGAT TGTCAAGAGC TGTCCCTCTG GATCAATGAA 
AAGATGCTTA CAGCTCAGGA CATGTCTTAT GATGAAGCCA GAAACCTGCA CAGTAAATGG 
TTAAAGCATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT 
GAGAAGGAAG GAATGCAGCT TATTTCAGAA AAGCCAGAAA CAGAGGCTGT GGTGAAGGAG 
AAACTCACTG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACAACCCA GACCAAGGCC 
CAGAGGCTCT TCGATGCGAA CAAGGCTGAG CTCTTCACGC AGAGCTGTGC AGACCTCGAC 
AAATGGCTGC ATGGCCTGGA GAGCCAGATT CAATCTGACG ACTATGGCAA AGACCTCACC 
AGTGTCAACA TTCTGCTGAA AAAGCAACAG ATGCTGGAGA ATCAGATGGA AGTGAGGAAG 
AAGGAGATCG AGGAACTGCA AAGCCAAGCC CAGGCACTGA GTCAGGAGGG GAAGAGCACA 
GATGAGGTGG ACAGCAAACG CCTCACTGTG CAGACCAAGT TCATGGAGCT TCTGGAGCCC 
TTGAATGAAA GGAAGCATAA TCTGCTGGCT TCCAAGGAGA TCCACCAGTT CAACAGGGAT 
GTAGAGGACG AGATTCTGTG GGTTGGCGAG AGGATGCCTT TGGCAACTTC CACAGATCAT 
GGCCACAACC TTCAAACTGT GCAGCTGTTA ATAAAGAAAA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGAC ACCAGCCTCG TATTGATGAC ATCTTTGAGA GGAGCCAAAA CATCATCACA 
GATAGCAGCA GCCTCAATGC CGAGGCTATC AGACAGAGGC TCGCCGATCT GAAGCAGCTG 
TGGGGGCTCC TCATTGAGGA AACTGAGAAA CGCCATAGAC GGCTCGAGGA GGCACACAAG 
GCCCAGCAAT ACTACTTTGA TGCAGCTGAA GCAGAGGCAT GGATGAGTGA ACAGGAACTG 
TACATGATGT CTGAGGAAAA GGCCAAGGAT GAGCAGAGTG CTGTCTCCAT GTTGAAAAAG 
CACCAGATTT TGGAACAGGC TGTTGAGGAC TATGCAGAGA CAGTGCACCA GCTCTCCAAG 
ACCAGCCGGG CACTGGTGGC TGACAGCCAT CCCGAAAGTG AGCGTATTAG CATGCGGCAG 
TCAAAGGTCG ACAAGCTGTA TGCTGGCCTG AAGGACCTTG CTGAGGAGAG GAGAGGGAAA 
CTTGATGAGA GGCACAGGCT ATTCCAGCTC AACAGGGAGG TAGATGACCT GGAACAGTGG 
ATCGCCGAGA GGGAAGTGGT TGCAGGCTCC CATGAGTTGG GACAGGACTA TGAGCATGTC 
ACGATGTTAC AAGAACGGTT CCGAGAATTT GCTCGAGACA CAGGAAACAT TGGGCAGGAG 
CGTGTAGATA CAGTTAACCA CATGGCAGAT GACCTCATCA ATTCTGGACA TTCAGATGCT 
GCGACTATTG CTGAGTGGAA AGATGGTCTC AATGAAGCCT GGGCTGACCT CCTGGAGCTC 
ATTGACACGA GAACACAGAT TCTTGCTGCC TCTTACGAAC TTCATAAGTT TTACCATGAT 
GCCAAGGAGA TCTTTGGCCG CATCCAGGAC AAACACAAGA AACTCCCTGA GGAGCTTGGA 
AGAGATCAGA ACACCGTGGA GACTTTACAG AGAATGCACA CCACCTTTGA ACATGACATC 
CAAGCTCTGG GCACTCAGGT GAGGCAGCTG CAGGAGGATG CAGCCCGCCT CCAAGCAGCC 
TATGCAGGGG ACAAGGCTGA CGACATCCAA AAGCGTGAGA ACGAGGTCCT GGAAGCCTGG 
AAGTCCCTGC TGGATGCTTG TGAGGGTCGC AGGGTGCGGC TAGTAGACAC GGGAGACAAG 
TTCCGCTTCT TCAGCATGGT GCGTGACCTC ATGCTCTGGA TGGAAGATGT CATCCGGCAA 
ATCGAGGCCC AGGAGAAACC CCGGGATGTG TCATCTGTTG AACTGTTAAT GAATAATCAT 
CAAGGCATCA AAGCTGAAAT CGATGCTCGT AATGACAGTT TTACAGCTTG CATTGAACTT 
GGGAAAGCCC TGCTGGCAAG GAAACACTAT GCTTCTGAGG AGATCAAGGA AAAGTTACTG 
CAGTTGACGG AGAAAAGAAA AGAAATGATC GACAAGTGGG AAGACCGATG GGAGTGGTTA 
AGACTGATTT TGGAGGTCCA TCAGTTCTCA AGAGATGCCA GTGTGGCTGA GGCTTGGCTG 
CTGGGACAGG AGCCATACCT ATCCAGCCGC GAAATCGGCC AGAGTGTGGA TGAAGTGGAG 
AAGCTTATTA AGCGCCATGA GGCATTTGAA AAATCTGCAG CGACCTGGGA TGAGAGATTC 
TCTGCCCTGG AAAGGTTGAC AACATTGGAG CTACTGGAAG TGCGCAGACA GCAAGAGGAA 
GAAGAAAGAA AGAGGCGACC ACCGTCTCCG GAGCCAAGCG CGAAGGTTTC AGAGGAGGCT 
GAGTCCCAGC AATGGGACAC TTCAAAAGGA GACCAAGTTT CCCAGAATGG TTTGCCGGCT 
GAGCAGGGAT CTCCACGGGT TAGTTACCGC TCTCAAACGT ACCAAAACTA CAAAAACTTT 
AATAGCAGAC GGACAGCCAG TGACCATTCG TGGTCTGGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sptbn1 ( XM_008770463.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768685.1 Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X6, mRNA.

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CloneID	ORa46318
Clone ID Related Accession (Same CDS sequence)	XM_008770463.2
Accession Version	XM_008770463.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	spectrin beta chain, non-erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGGAT 
AACATCATAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768685.1
CDS	126..6557
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAESYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSEKIRERISYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEISNYRPTIDTLHEQAGALPQAHAESPDVKGRLAGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLNERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIELGKALLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPEPSAKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRDNII

Target ORF information:

RefSeq Version	XM_008770463.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spectrin, beta, non-erythrocytic 1 (Sptbn1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770463.2

ORF Insert Sequence:

ATGACGACCA CGGTGGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
GTGAACAACC GCTGGGACGT GGATGACTGG GACAATGAGA ACAGCTCTGC ACGGCTCTTT 
GAGCGCTCCC GCATCAAGGC CCTGGCAGAT GAGCGTGAAG CTGTACAGAA GAAGACCTTC 
ACCAAGTGGG TCAACTCCCA CCTTGCCCGA GTGTCCTGCC GAATCACAGA CCTGTACACT 
GACCTTCGAG ATGGACGGAT GCTCATCAAA CTACTGGAGG TCCTCTCTGG AGAGAGGCTG 
CCTAAACCCA CTAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTCGA CAAGGCTCTT 
CAGTTCCTGA AGGAGCAGAG AGTCCATCTC GAGAACATGG GCTCCCATGA TATTGTGGAT 
GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATTA TCCTGCGCTT CCAGATCCAG 
GATATCAGTG TGGAGACTGA AGATAACAAA GAGAAAAAGT CTGCTAAGGA TGCATTGCTG 
CTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCTAATGTCA ACATTCACAA TTTCACCACT 
AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA 
GATTTTGATA AACTGAAGAA ATCGAATGCA CACTACAATC TGCAGAATGC ATTTAACCTG 
GCAGAGCAGC ACCTTGGCCT TACTAAACTG TTAGACCCTG AAGATATCAG CGTGGACCAC 
CCTGATGAGA AGTCCATCAT CACGTATGTG GTCACTTACT ACCACTACTT CTCCAAGATG 
AAGGCCTTGG CTGTCGAAGG AAAGCGCATT GGGAAGGTGC TTGATAATGC TATAGAAACA 
GAGAAAATGA TTGAGAAGTA TGAGTCGCTT GCTTCTGACC TTCTGGAGTG GATTGAACAA 
ACCATCATCA TTCTAAACAA CCGCAAATTT GCTAATTCAC TGGTTGGGGT CCAGCAGCAG 
CTTCAGGCAT TCAACACGTA CCGCACAGTG GAGAAACCAC CTAAGTTTAC TGAGAAGGGG 
AATTTGGAGG TGCTGCTTTT CACAATTCAG AGCAAGATGA GAGCTAATAA TCAGAAGGTC 
TACATGCCCC GAGAAGGGAA GCTCATCTCT GATATCAACA AGGCCTGGGA AAGACTGGAA 
AAAGCAGAAC ATGAGAGAGA ACTGGCTCTG CGGAATGAGC TCATACGGCA GGAAAAACTG 
GAACAACTTG CCCGCAGATT CGATCGCAAG GCAGCTATGA GAGAGACATG GCTGAGCGAA 
AACCAGCGTC TTGTCTCTCA GGACAACTTT GGATTTGACC TTCCTGCAGT TGAGGCCGCC 
ACCAAAAAAC ATGAGGCCAT CGAGACTGAC ATTGCGGCGT ATGAAGAGCG AGTGCAGGCT 
GTGGTGGCTG TGGCCAGGGA GCTTGAAGCA GAGAGCTATC ATGACATCAA GCGCATCACA 
GCGAGGAAGG ACAATGTCAT CCGCCTCTGG GAGTACTTGC TGGAACTGCT CAGGGCCAGG 
AGGCAGCGTC TTGAGATGAA CCTGGGACTG CAAAAGATAT TCCAGGAAAT GCTTTATATT 
ATGGACTGGA TGGATGAAAT GAAGGTGCTA TTGCTGTCTC AGGACTATGG CAAACACTTG 
CTCGGTGTTG AAGACTTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGCAATCCAA 
GCAGAACGTG TGAGAGGTGT GAATGCCTCT GCCCAGAAGT TCGCAACAGA TGGGGAAGGC 
TACAAGCCAT GCGACCCCCA GGTAATCCGA GACCGTGTGG CCCACATGGA ATTCTGCTAT 
CAAGAACTCT GTCAGCTGGC AGCTGAGCGT AGGGCTCGCC TGGAAGAGTC TCGTCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAGGAG GAAGGCTGGA TACGGGAGAA GGAGAAGATC 
CTGTCCTCTG ATGACTATGG GAAAGACCTG ACTAGTGTCA TGCGCCTGCT GAGCAAGCAC 
AGGGCTTTTG AGGATGAGAT GAGTGGCCGT AGTGGCCATT TTGAGCAGGC CATTAAAGAA 
GGTGAAGACA TGATTGCAGA GGAACACTTT GGATCAGAAA AGATCCGTGA GAGAATCAGT 
TACATTCGAG AGCAGTGGGC CAACCTGGAG CAGCTCTCAG CCATTAGGAA GAAGCGCCTA 
GAGGAGGCCT CATTGCTGCA CCAGTTCCAG GCTGATGCTG ATGACATCGA CGCTTGGATG 
TTAGATATAC TCAAGATTGT GTCCAGCAAC GATGTGGGCC ATGATGAGTA TTCCACACAG 
TCTCTGGTCA AGAAGCATAA AGATGTGGCA GAAGAGATCT CCAACTACAG ACCCACTATT 
GACACACTGC ATGAGCAAGC CGGTGCTCTT CCTCAAGCAC ACGCAGAGTC TCCAGATGTG 
AAGGGCCGGC TGGCAGGAAT TGAGGAGCGT TACAAGGAGG TGGCAGAGTT AACACGGCTA 
AGGAAGCAGG CACTGCAGGA CACCCTGGCT CTGTACAAGA TGTTCAGTGA GGCTGATGCC 
TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG 
CTGGAGGACC TGGAAGTCAT CCAGCACAGA TTTGAGAGCC TAGAACCAGA AATGAACAAC 
CAGGCTTCCC GGGTTGCTGT GGTGAACCAG ATTGCACGGC AGCTGATGCA CAGTGGCCAT 
CCCAGCGAAA AGGAAATCAG AGCTCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC 
AGAGAACTGG TGGACAGGAA GAAGGATGCT CTTCTGTCCG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAATGAAAC CAAATCCTGG ATCCGGGAGA AGACCAAGGT CATCGAGTCT 
ACCCAAGACC TTGGCAATGA CCTGGCAGGT GTCATGGCCC TGCAGCGCAA GCTGACCGGC 
ATGGAACGAG ACTTGGTAGC CATCGAGGCG AAGCTGAGTG ACCTGCAGAA GGAAGCCGAG 
AAGCTTGAGT CCGAGCACCC TGACCAGGCT CAAGCCATCC TGTCTCGACT GGCTGAGATC 
AGTGACGTGT GGGAGGAGAT GAAGACAACC CTGAAGAACC GAGAAGCCTC GCTGGGAGAG 
GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC TTGGATGACT TCCAGTCTTG GCTCTCCAGG 
ACCCAGACTG CTATTGCCTC AGAGGACATG CCCAATACCC TGACTGAGGC AGAGAAGCTT 
CTCACACAGC ATGAGAACAT CAAAAACGAG ATTGACAATT ACGAGGAAGA CTACCAGAAG 
ATGCGGGACA TGGGCGAGAT GGTCACGCAG GGACAGACTG ATGCCCAGTA CATGTTTCTG 
CGGCAGCGGT TGCAGGCCTT GGACACAGGC TGGAACGAGC TCCACAAAAT GTGGGAGAAC 
AGGCAAAATC TCCTCTCCCA GTCCCATGCC TACCAGCAGT TCCTTAGGGA CACGAAACAG 
GCTGAAGCTT TCCTTAATAA CCAGGAGTAT GTTTTGGCTC ATACTGAAAT GCCCACCACC 
CTGGAAGGAG CTGAAGCAGC CATTAAGAAG CAGGAGGACT TCATGACCAC CATGGATGCC 
AATGAGGAGA AGATCAATGC TGTTGTGGAG ACTGGCCGGA GGCTGGTGAG CGATGGGAAT 
ATCAACTCCG ACCGCATCCA GGAGAAGGTG GACTCTATTG ATGACAGGCA CAGAAAGAAT 
CGAGAAGCAG CCAGTGAACT TTTGATGAGG TTAAAGGACA ACCGTGATCT ACAGAAGTTC 
CTGCAAGATT GTCAAGAGCT GTCCCTCTGG ATCAATGAAA AGATGCTTAC AGCTCAGGAC 
ATGTCTTATG ATGAAGCCAG AAACCTGCAC AGTAAATGGT TAAAGCATCA AGCATTTATG 
GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTT 
ATTTCAGAAA AGCCAGAAAC AGAGGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAA 
ATGTGGGAAG TCCTTGAATC CACAACCCAG ACCAAGGCCC AGAGGCTCTT CGATGCGAAC 
AAGGCTGAGC TCTTCACGCA GAGCTGTGCA GACCTCGACA AATGGCTGCA TGGCCTGGAG 
AGCCAGATTC AATCTGACGA CTATGGCAAA GACCTCACCA GTGTCAACAT TCTGCTGAAA 
AAGCAACAGA TGCTGGAGAA TCAGATGGAA GTGAGGAAGA AGGAGATCGA GGAACTGCAA 
AGCCAAGCCC AGGCACTGAG TCAGGAGGGG AAGAGCACAG ATGAGGTGGA CAGCAAACGC 
CTCACTGTGC AGACCAAGTT CATGGAGCTT CTGGAGCCCT TGAATGAAAG GAAGCATAAT 
CTGCTGGCTT CCAAGGAGAT CCACCAGTTC AACAGGGATG TAGAGGACGA GATTCTGTGG 
GTTGGCGAGA GGATGCCTTT GGCAACTTCC ACAGATCATG GCCACAACCT TCAAACTGTG 
CAGCTGTTAA TAAAGAAAAA CCAGACCCTC CAGAAAGAAA TCCAGGGACA CCAGCCTCGT 
ATTGATGACA TCTTTGAGAG GAGCCAAAAC ATCATCACAG ATAGCAGCAG CCTCAATGCC 
GAGGCTATCA GACAGAGGCT CGCCGATCTG AAGCAGCTGT GGGGGCTCCT CATTGAGGAA 
ACTGAGAAAC GCCATAGACG GCTCGAGGAG GCACACAAGG CCCAGCAATA CTACTTTGAT 
GCAGCTGAAG CAGAGGCATG GATGAGTGAA CAGGAACTGT ACATGATGTC TGAGGAAAAG 
GCCAAGGATG AGCAGAGTGC TGTCTCCATG TTGAAAAAGC ACCAGATTTT GGAACAGGCT 
GTTGAGGACT ATGCAGAGAC AGTGCACCAG CTCTCCAAGA CCAGCCGGGC ACTGGTGGCT 
GACAGCCATC CCGAAAGTGA GCGTATTAGC ATGCGGCAGT CAAAGGTCGA CAAGCTGTAT 
GCTGGCCTGA AGGACCTTGC TGAGGAGAGG AGAGGGAAAC TTGATGAGAG GCACAGGCTA 
TTCCAGCTCA ACAGGGAGGT AGATGACCTG GAACAGTGGA TCGCCGAGAG GGAAGTGGTT 
GCAGGCTCCC ATGAGTTGGG ACAGGACTAT GAGCATGTCA CGATGTTACA AGAACGGTTC 
CGAGAATTTG CTCGAGACAC AGGAAACATT GGGCAGGAGC GTGTAGATAC AGTTAACCAC 
ATGGCAGATG ACCTCATCAA TTCTGGACAT TCAGATGCTG CGACTATTGC TGAGTGGAAA 
GATGGTCTCA ATGAAGCCTG GGCTGACCTC CTGGAGCTCA TTGACACGAG AACACAGATT 
CTTGCTGCCT CTTACGAACT TCATAAGTTT TACCATGATG CCAAGGAGAT CTTTGGCCGC 
ATCCAGGACA AACACAAGAA ACTCCCTGAG GAGCTTGGAA GAGATCAGAA CACCGTGGAG 
ACTTTACAGA GAATGCACAC CACCTTTGAA CATGACATCC AAGCTCTGGG CACTCAGGTG 
AGGCAGCTGC AGGAGGATGC AGCCCGCCTC CAAGCAGCCT ATGCAGGGGA CAAGGCTGAC 
GACATCCAAA AGCGTGAGAA CGAGGTCCTG GAAGCCTGGA AGTCCCTGCT GGATGCTTGT 
GAGGGTCGCA GGGTGCGGCT AGTAGACACG GGAGACAAGT TCCGCTTCTT CAGCATGGTG 
CGTGACCTCA TGCTCTGGAT GGAAGATGTC ATCCGGCAAA TCGAGGCCCA GGAGAAACCC 
CGGGATGTGT CATCTGTTGA ACTGTTAATG AATAATCATC AAGGCATCAA AGCTGAAATC 
GATGCTCGTA ATGACAGTTT TACAGCTTGC ATTGAACTTG GGAAAGCCCT GCTGGCAAGG 
AAACACTATG CTTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA GAAAAGAAAA 
GAAATGATCG ACAAGTGGGA AGACCGATGG GAGTGGTTAA GACTGATTTT GGAGGTCCAT 
CAGTTCTCAA GAGATGCCAG TGTGGCTGAG GCTTGGCTGC TGGGACAGGA GCCATACCTA 
TCCAGCCGCG AAATCGGCCA GAGTGTGGAT GAAGTGGAGA AGCTTATTAA GCGCCATGAG 
GCATTTGAAA AATCTGCAGC GACCTGGGAT GAGAGATTCT CTGCCCTGGA AAGGTTGACA 
ACATTGGAGC TACTGGAAGT GCGCAGACAG CAAGAGGAAG AAGAAAGAAA GAGGCGACCA 
CCGTCTCCGG AGCCAAGCGC GAAGGTTTCA GAGGAGGCTG AGTCCCAGCA ATGGGACACT 
TCAAAAGGAG ACCAAGTTTC CCAGAATGGT TTGCCGGCTG AGCAGGGATC TCCACGGGAT 
AACATCATAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.
Scientific reports626725(2016 May)
Sidenstein SC,D'Este E,B?hm MJ,Danzl JG,Belov VN,Hell SW

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton.
Journal of immunology (Baltimore, Md. : 1950)192(2)771-81(2014 Jan)
Forster F,Paster W,Supper V,Schatzlmaier P,Sunzenauer S,Ostler N,Saliba A,Eckerstorfer P,Britzen-Laurent N,Sch?tz G,Schmid JA,Zlabinger GJ,Naschberger E,St?rzl M,Stockinger H

Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons.
Science (New York, N.Y.)339(6118)452-6(2013 Jan)
Xu K,Zhong G,Zhuang X

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Sptbn1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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