beta-Spec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	beta-Spec
Entrez Gene ID	32746
Full Name	beta Spectrin
Synonyms	B-spec,CG5870,Dmel\CG5870,SPEC8,Spec,Spec-beta,Specbeta,anon-EST:fe1B3,b spectrin,b-Spec,beta,beta-spec,betaSpec,betaspec,i168,i6,kus,l(1)G0074,l(1)G0108,l(1)G0198
General protein information	Preferred Names beta Spectrin Names CG5870 gene product from transcript CG5870-RC CG5870-PA CG5870-PB CG5870-PC anon-fast-evolving-1B3 beta spectrin beta-Spec-PA beta-Spec-PB beta-Spec-PC beta-spectrin karussell spectrin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 16B10-16B12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272731.1	NP_001259660.1	beta spectrin, isoform B
NM_078664.3	NP_523388.1	beta spectrin, isoform A
NM_001272732.1	NP_001259661.1	beta spectrin, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPTBN1	NP_001186354.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010895	XP_309796.2
Caenorhabditis elegans (roundworm)	unc-70	NP_001024053.1
Canis lupus familiaris (dog)	SPTBN1	XP_531827.2
Pan troglodytes (chimpanzee)	SPTBN1	XP_001154155.1
	SPTBN1	XP_001114804.1
Bos taurus (cattle)	SPTBN1	NP_001179205.1
Drosophila melanogaster (fruit fly)	beta-Spec	NP_001259660.1
Homo sapiens (human)	SPTBN1	NP_003119.2
Mus musculus (house mouse)	Sptbn1	NP_787030.2
Rattus norvegicus (Norway rat)	Sptbn1	NP_001013148.1
Danio rerio (zebrafish)	sptb	NP_571600.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The abnormal stress fiber-like actin structure on the basal side of follicle cells provides a likely link between the b-spectrin mutations and the loss of the Hippo signaling activity phenotype.
Title: ?-Spectrin regulates the hippo signaling pathway and modulates the basal actin network.

Model in which alphabeta-spectrin directly couples lipid uptake at the plasma membrane to lipid droplet growth in the cytoplasm. In contrast, strong overexpression of beta-spectrin caused fat body atrophy and larval lethality.
Title: Genetic studies of spectrin in the larval fat body of Drosophila melanogaster: evidence for a novel lipid uptake apparatus.

Data show that DAnk2-binding is critical for beta spectrin function in vivo.
Title: Transgene rescue identifies an essential function for Drosophila beta spectrin in the nervous system and a selective requirement for ankyrin-2-binding activity.

beta-Spectrin is required in neurons for proper midline axon guidance in the embryonic CNS.
Title: beta-Spectrin functions independently of Ankyrin to regulate the establishment and maintenance of axon connections in the Drosophila embryonic CNS.

At the Drosophila neuromuscular junction postsynaptic Spectrin-actin lattice acts as an organizing scaffold upon which pre- and postsynaptic development are arranged.
Title: A postsynaptic spectrin scaffold defines active zone size, spacing, and efficacy at the Drosophila neuromuscular junction.

The following beta-Spec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the beta-Spec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01925	NM_078664.3 Latest version!	Drosophila melanogaster beta spectrin, transcript variant A (beta-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03704	NM_001272732.1 Latest version!	Drosophila melanogaster beta spectrin, transcript variant C (beta-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03705	NM_001272731.1 Latest version!	Drosophila melanogaster beta spectrin, transcript variant B (beta-Spec), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

beta-Spec ( NM_078664.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523388.1 Drosophila melanogaster beta spectrin, transcript variant A (beta-Spec), mRNA.

Price & Availability↑

CloneID	OFa01925
Clone ID Related Accession (Same CDS sequence)	NM_078664.3
Accession Version	NM_078664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta spectrin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078664.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGG ACATTTCGAT TGTTCGCTGG GATCCCAGCC AGGGTCCTGG CAACGAGTAC 
ATCGATGAGT ACGAGTACGA TGGCGGCAAC TCCAGTTCCC GACTGTTCGA GCGGTCCAGG 
ATCAAGGCTC TGGCCGAGGA GCGTGAGAGT GTGCAAAAGA AGACATTCAC CAAGTGGGTC 
AATTCGCATC TGTGCCGGGT CAACTGCCGC ATTGCCGATC TCTATGTGGA TATGCGGGAT 
GGCAAGCACC TGATCAAACT GCTGGAGGTC CTCTCCGGCG AGCGACTGCC CAAGCCCACC 
AAGGGCAAGA TGCGTATCCA CTGCCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGCGC 
GAACAGCGCG TTCATCTGGA GAATATTGGC TCCCATGACA TAGTCGATGG CAATGCTTCC 
CTCAATTTGG GCCTGATTTG GACCATCATC CTGCGCTTCC AGATCCAGGA CATCACCATC 
GAGGAGGTGG ACAACAAGGA GACCAAGTCC GCCAAGGACG CCCTGCTGCT CTGGTGCCAG 
ATGAAGACTG CCGGCTATCA CAATGTGAAC GTGCGCAACT TCACCACCTC GTGGCGCGAT 
GGCCTGGCCT TTAATGCCAT CATCCACAAA CACCGTCCGG ATTTGGTTCA GTTCGAGAAA 
CTCTCGAAGA CGAACGCCAT CCACAACCTA AACAATGCCT TCGACGTGGC CGAGGACAAA 
CTGGGCCTGG CCAAGCTCCT TGATGCCGAG GATGTGTTCG TCGAGCATCC GGACGAGAAG 
TCCATCATCA CCTACGTGGT CACCTACTAT CACTACTTTA GCAAACTCAA GCAGGAGACG 
GTGCAGGGCA AGCGTATCGG TAAGGTGGTC GGCATTGCCA TGGAGAACGA CAAAATGGTC 
CACGACTACG AGAACTTCAC AAGCGATCTG CTCAAGTGGA TCGAAACGAC CATCCAGTCG 
CTGGGCGAGC GGGAGTTCGA AAACTCGCTG GCCGGCGTCC AAGGGCAGTT GGCCCAGTTC 
TCCAACTACC GCACCATCGA GAAGCCGCCC AAGTTTGTGG AAAAGGGCAA CCTCGAGGTG 
CTCCTTTTCA CCCTGCAGTC CAAGATGCGG GCCAACAACC AGAAGCCCTA CACACCCAAA 
GAGGGCAAGA TGATTTCGGA CATCAACAAG GCCTGGGAGC GTCTGGAGAA GGCCGAGCAC 
GAACGCGAAT TGGCCCTGCG CGAGGAGCTC ATCCGGCAGG AGAAACTGGA GCAGCTAGCC 
GCCCGCTTCG ACCGCAAGGC GTCCATGCGT GAGACCTGGC TGTCGGAGAA TCAACGTCTG 
GTCAGCCAGG ATAACTTTGG TTTCGATCTG GCGGCCGTTG AGGCGGCGGC CAAGAAGCAC 
GAAGCCATCG AAACCGACAT CTTCGCCTAC GAGGAGCGCG TCCAGGCCGT GGTTGCCGTT 
TGCGATGAAC TGGAATCGGA GCGTTATCAC GATGTGAAGC GCATCCTTCT GCGCAAGGAC 
AACGTTATGC GCTTGTGGAC CTATTTGTTG GAGCTTCTGA GAGCGCGCCG CATGCGCCTG 
GAGATCTCGT TGCAGCTGCA GCAGAACTTC CAGGAGATGC TGTACATCCT CGACAACATG 
GAGGAGATCA AGCAGCTGCT GATGACCGAC GATTATGGCA AGCATCTGAT GGGCGTGGAG 
GATCTGCTGC AGAAGCACTC GCTTGTCGAA GCCGATATCA ATATCCTGGG CGAGCGCGTC 
AAGGTGGTTG TTCAGAACTC GCAGAAGTTC CTGAGCGACG ACCCAGAGTC GTACAAACCG 
TGCGATCCCG AGATCATTGT CTCGCGCGTC CAGCAACTGG AGGATGCTTA CGCAGAGTTG 
GTCCGTTTGG CCGTGGAACG TCGCAGCCGC CTGGAGGAGA GCCGCAAACT CTGGCAGTTC 
TACTGGGACA CCGCCGACGA GGAGAACTGG ATCAAGGAGA AGGAGCAGAT CGTCTCCACC 
GACGAGGTGG GTCACGACCT GACCACCGTC AATCTGATGC TCAGCAAGCA CAAGGCCCTG 
GAGTCGGAGA TCACGTCGCA CGATCCCCAG CTGCAAAATG TGGCCAAGGT CGGTTCGGAG 
CTGATCACCG AGGGTCACTT TGGTGCCGAT CGCATCAAGG ATCGATTGAA GGAGATCCTC 
AACAAGTGGG ACCACCTTCT GGACCTCACC AAGTACCGCC GCCAGCGCTT GGAGAATGCC 
GTCGAGTACT TCCAGCTGTT TGCCGATGCC GACGATGTGG ACAACTGGAT GTTGGACACC 
CTGCGCATTG TTTCCAGCGA GGATGTTGGT CGCGACGAGG CTAACGTACA GTCCCTCCTA 
AAGAAGCACA AGGACGTGGC CGACGAGCTG AAGAATTACG CCGAAGTGAT CGATGCCCTG 
CACAAACAGG CGGAGAGCCT GAAGCTAAAC GAGGCGGAAA AGGCCAATGT GGACAAACGC 
CTGGAGGCGA TCGACAACAG GTACAAGGAG CTTACCGAAC TGGCCAAGCT GCGCAAGCAG 
CGACTGCTCG ACGCCCTCAG CCTGTACAAG CTGATGTCCG AAGCGGATGG CGTGGAGCAA 
TGGATCAAGG AGAAGACCAA GATGCTGGAC ACGATGACTC CTGGCAAGGA CATCGAGGAT 
GTGGAGATCA TGAAGCATCG CTTCGAGGGC TTCGACAAGG AAATGAACGC CAATGCCTCG 
CGCGTGGCCG TCGTTAATCA GCTGGCCCGC CAGCTGCTCC ACGTCGAGCA TCCCAACTCC 
GATGAGATTC TGGAGAGGCA GAACCACCTC AACCAGGAGT GGTCGACACT GCGCGAGAAG 
GCCGAGGCCA AGATGGATGA TCTGAAGTCC GCCCATGGCG TCCAGACCTT CTACATTGAG 
TGCCGCGAGA CCATTTCGTG GATTGAGGAT AAGAAGCGCA TCCTTACCGA GACCGACAGT 
CTGGAGATGG ATCTGACCGG TGTGATGACC CTGCAGCGTC GCCTTAGCGG CATGGACCGC 
GACTTGGCCG CCATTCAGGC CAAGCTCTCG AGCTTGGAGC GCGAAGCCAA CAGCATCGAG 
GATGAGCATC CTGAGGAGGC CAAGATCATC CGCGAGCGCA TTGCCCAGAT CGAGTTGATT 
TGGGAGCAGC TCACCCAGAT GCTCAAGGAG CGCGACTCGA AACTGGAGGA GGCCGGCGAT 
CTGCATCGCT TCTTGCGCGA TCTGGATCAC TTCCAGACTT GGTTGACCAA GACCCAGACA 
GATGTAGCTT CCGAGGACAC TCCCACTTCC CTGCCGGAGG CTGAGAAGCT TCTGAACCAG 
CATCAATCGA TCCGCGAGGA GATCGACAAC TACACTGAGG ACTACAAGAA CATGATGGAG 
TACGGCGAGC GACTGACCTC CGAGGGAAGC ACCTCAGACG ATCCACAGTA CATGTTCTTG 
AGGGAGCGCC TGAATGCCCT GAAGGATGGC TGGGAGGAGC TGCACCAGAT GTGGGAGAAC 
AGACAGGTGC TGTTGTCGCA GAGCTTGGAC CAGCAACTGT TCAACCGCGA TGCCCGCCAG 
ACGGAGGTGT TGCTCAGCCA ACAGGAGCAC TTCCTCAGCA AGGATGATAC CCCCGTCAAC 
CTGGAGCAGG CTGAGAACCA GTTGAAGCGC CACGAGGCCT TCCTCACCAC CATGGAAGCT 
AACGACGACA AGATCAACAC CCTGCTGCAG GTGGCCGACA CCCTGGTGGA AAAGGATCAC 
TTCGATGCGG ACAAGATTGG CAAGCGTGCG GAGAACATTA CCGGACGTCG GGATGACAAC 
CGTCAGCGTG CTCTGGACCA ACACGAGAAG CTGAAGAATC AGGTGAAACT GCACGAGTTC 
CTGCAGGATC TCGAAGAGCT GGCCGAGTGG GTGCAGGAGA AATACGCCAC TTCACAGGAC 
GAGTCGTACA GGAGCGCAAA GACCATCCAC TCCAAGTGGA CGCGCCATCA GGCCTTCGAG 
GCAGAGATTG CCGCCAACAA GGAGCGCCTA TTCGAGGCAG AGAAGTCTGC CCAGGAGCTA 
TCCAAGGAGA AGCCCGAGTT CAAGGACGTC ATCGAGCCCA AGCTTAAGGA GCTGGCGAAG 
CAATTCGACG ACCTGGAGGT GCACACCAAG GAGAAGGGCG CCATGCTGTT CGACGCCAAC 
CGCGAGGTGC TTGTCCAGCA GACATGCGAC GACATCGACT CCTACATCAC CGACCTGGAG 
AAGCAAATTG TCAGCGGAGA CACTGCCAAC GATCTGACAT CCGTGAACAT CCTGATGCAG 
AAGCAGCAGG TTATCCAGAC CCAGATGGCG GTGAAGGCTC GCCAAGTGGA GGAGATCGAC 
AAGCAGACCG AATACCTCCA GAAGACTGTG CCCGAGGAGA AGATCGAACC GATTGTGGTG 
AAGAAGACGG CCGTGCTCGA GCGCTTTGAG AAGATCAAGG CGCCGCTTCT GGAGCGCCAG 
AAGGCGCTGG AGAAGAAGAA GGAGGCCTTC CAGTTCTGTC GCGATGTCGA GGACGAGAAG 
CTCTGGATTG ACGAGAAGCT GCCGGTGGCC AACTCACCGG ATTACGGCAA CTCCCTGTTC 
AACGTACATG TGCTGAAGAA GAAGAACCAG TCGCTGGCCA CAGAGATCGA CAACCACGAG 
CCGCGCATCA ATGCAATCTG CAACAATGGC CGCAAGCTGA TCGACGAGGG CCACGAGGAT 
GCCAAGAAGT TCGAGGCGCT GATCAGCGAT CTGACCCAGA AGTGGCAGGA GCTGAAGGAC 
GCCATCGAGA ACCGACGGAA GCATCTGCTG GAGTCGGAGA AGGTGCAGCA GTACTTCTTC 
GATGCCCAGG AGGCGGAGTC CTGGATGAGC GAGCAGGAGC TCTACATGAT GGTCGAGGAT 
CGCGGCAAGG ACGAGATCAG TGCCCAGAAC CTGATGAAGA AGCACGAAAA CCTGGAGCAA 
TCCGTAGAGG ACTACGCCAA CACCATTCGC CAGCTCGGCG AGGTGGCGCG TCAGTTCAGC 
GGGGATGACA TCTCCTCCGG CGATGCCGTG GCCGTCAAGC AATCGCAGCT GGATAAGCTT 
TACGCTGGTC TCAAGGATCT GGCTGGGGAG AGGAGGGCTC GCCTCAACGA GGCTCTGCAG 
CTGTTCATGC TGAGCCGCGA GGTCGATGAT CTGGAGCAAT GGATTACCGA TCGCGAGGTT 
GTCGCCGGTT CCCAGGAATT GGGCCAGGAC TTTGACCACG TCACTCTGCT GTCGGAGCGC 
TTCAACGAGT TTGCCCGCGA CACAGAGGCC GTTGGCGGCG AGCGAGTGGC CAAGGTGAAC 
GGCATTGCCG ATAATCTTAT CCAGGCCGGA CACTCTGATT CCGCCACGAT TGCCGAATGG 
AAGGACAACC TCAACGAATC GTGGCAGGAT CTGCTGGAGC TGATCGAGAC ACGCACTCAG 
ATGCTGGCCG CTTCTCGGGA GCTGCACAAA TTCTTCCACG ACTGCAAGGA TGTGTTGGGA 
CGCATCCTCG AGAAGCAGCA CGGTGTGTCC GATGAACTGG GACGCGATGC CGGCTCCGTG 
TCGACGCTGC AGCGCAAGCA CTACAACTTC CTGCAGGACC TCATCACCCT ATACTCACAA 
GTCCAGCAGA TCCAGGAGGA ATCCGCCAAG CTGCAGGACG CCTATGCCGG TGATAAGGCC 
AAGGAAATCA CCAATCGCGA ACAGGAGGTG CTCCATGCCT GGGACAACCT CCAGGCTATG 
TGCGATGCCC GCAAACAGAA ACTGGCCGAC ACAGGCGACT TATTCCGCTT CTTCAACATG 
GTGCGCATCC TGATGATCTG GATGGAGGAC CTCGTCCGCC AAATGAACAC ATCAGAGAAG 
CCGCGCGACG TGTCCGGCGT GGAGCTGCTG ATGAACAACC ACCAGAGCCT TAAGGCCGAG 
ATCGACACAC GGGAGGACAA CTTCGGTGCG TGCATCTCGC TGGGCAAGGA GCTGCTCACC 
CGCAACCACT ACGCCTCCGC CGATATCAAG GATCGCCTGA TGACGCTGAG CAATAGCCGC 
AATGCCCTGC TCCGCCGCTG GGAGGAGCGC TGGGAGAATC TCCAGCTGAT CCTGGAGGTG 
TACCAGTTCG CCAGAGACGC TGCCGTGGCC GAGGCCTGGC TGATCGCCCA GGAGCCTTAC 
CTGCTCTCTT CGGAGCTGGG CCACACAATC GACGAGGTGG AGAACCTGAT TAAGAAGCAC 
GAGGCCTTTG AAAAGTCTGC CGCCGCCCAA GAGGAGCGCT TCAGTGCTCT TGAGCGTTTG 
ACAACATTTG AGCTTAAGGA AATGAAGAGG CGCCAGGAGC TGGCGGAAGA GGCTGAGCGA 
CAACGCATCA AGGAGGAGCA GGAGGCCAAG GCCGCCTCGG AGGCGGCGGA ACAGGCTAAA 
CGAGAGGCTG AGCGACGCGA CGACGTGGAT GTTGGTGCCT CGCACGACGA TTCAGAACGA 
GGCGGAACCC CAGGCGCCGG CGAGGGTCAC GAAGGATATG TGACACGAAA GCACGAGTGG 
GACTCGACCA CCAAGAAGGC CTCCAACCGA TCTTGGGATA AGGTATACAT GGCTGCCAAG 
GCCGGACGCA TTTCGTTCTA CAAAGATCAG AAGGGTTATA AGAGTAATCC CGAATTGACC 
TTCCGCGGTG AGCCCAGCTA CGATCTGCAA AACGCTGCCA TCGAAATTGC CAGTGATTAC 
ACCAAGAAGA AGCACGTTCT GCGAGTCAAG CTCGCCAACG GTGCCTTGTT CTTGCTGCAG 
GCCCATGACG ACACCGAGAT GTCCCAGTGG GTGACCTCCC TGAAAGCCCA GAGCGATTCG 
ACAGCGGTGG CCGCCAGCAG ATCCCAGACT CTGCCCGCCA CCTCACAAAA GGACGAACCA 
AAGCGCAGAT CGTTTTTTAC TTTAAAGAAA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523388.1
CDS	362..7237
Translation	MTTDISIVRWDPSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGIAMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAGVQGQLAQFSNYRTIEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAARFDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCDELESERYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQQNFQEMLYILDNMEEIKQLLMTDDYGKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLSDDPESYKPCDPEIIVSRVQQLEDAYAELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQIVSTDEVGHDLTTVNLMLSKHKALESEITSHDPQLQNVAKVGSELITEGHFGADRIKDRLKEILNKWDHLLDLTKYRRQRLENAVEYFQLFADADDVDNWMLDTLRIVSSEDVGRDEANVQSLLKKHKDVADELKNYAEVIDALHKQAESLKLNEAEKANVDKRLEAIDNRYKELTELAKLRKQRLLDALSLYKLMSEADGVEQWIKEKTKMLDTMTPGKDIEDVEIMKHRFEGFDKEMNANASRVAVVNQLARQLLHVEHPNSDEILERQNHLNQEWSTLREKAEAKMDDLKSAHGVQTFYIECRETISWIEDKKRILTETDSLEMDLTGVMTLQRRLSGMDRDLAAIQAKLSSLEREANSIEDEHPEEAKIIRERIAQIELIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQTWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTEDYKNMMEYGERLTSEGSTSDDPQYMFLRERLNALKDGWEELHQMWENRQVLLSQSLDQQLFNRDARQTEVLLSQQEHFLSKDDTPVNLEQAENQLKRHEAFLTTMEANDDKINTLLQVADTLVEKDHFDADKIGKRAENITGRRDDNRQRALDQHEKLKNQVKLHEFLQDLEELAEWVQEKYATSQDESYRSAKTIHSKWTRHQAFEAEIAANKERLFEAEKSAQELSKEKPEFKDVIEPKLKELAKQFDDLEVHTKEKGAMLFDANREVLVQQTCDDIDSYITDLEKQIVSGDTANDLTSVNILMQKQQVIQTQMAVKARQVEEIDKQTEYLQKTVPEEKIEPIVVKKTAVLERFEKIKAPLLERQKALEKKKEAFQFCRDVEDEKLWIDEKLPVANSPDYGNSLFNVHVLKKKNQSLATEIDNHEPRINAICNNGRKLIDEGHEDAKKFEALISDLTQKWQELKDAIENRRKHLLESEKVQQYFFDAQEAESWMSEQELYMMVEDRGKDEISAQNLMKKHENLEQSVEDYANTIRQLGEVARQFSGDDISSGDAVAVKQSQLDKLYAGLKDLAGERRARLNEALQLFMLSREVDDLEQWITDREVVAGSQELGQDFDHVTLLSERFNEFARDTEAVGGERVAKVNGIADNLIQAGHSDSATIAEWKDNLNESWQDLLELIETRTQMLAASRELHKFFHDCKDVLGRILEKQHGVSDELGRDAGSVSTLQRKHYNFLQDLITLYSQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHAWDNLQAMCDARKQKLADTGDLFRFFNMVRILMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFGACISLGKELLTRNHYASADIKDRLMTLSNSRNALLRRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLLSSELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRRQELAEEAERQRIKEEQEAKAASEAAEQAKREAERRDDVDVGASHDDSERGGTPGAGEGHEGYVTRKHEWDSTTKKASNRSWDKVYMAAKAGRISFYKDQKGYKSNPELTFRGEPSYDLQNAAIEIASDYTKKKHVLRVKLANGALFLLQAHDDTEMSQWVTSLKAQSDSTAVAASRSQTLPATSQKDEPKRRSFFTLKKK

Target ORF information:

RefSeq Version	NM_078664.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta spectrin, transcript variant A (beta-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078664.3

ORF Insert Sequence:

ATGACGACGG ACATTTCGAT TGTTCGCTGG GATCCCAGCC AGGGTCCTGG CAACGAGTAC 
ATCGATGAGT ACGAGTACGA TGGCGGCAAC TCCAGTTCCC GACTGTTCGA GCGGTCCAGG 
ATCAAGGCTC TGGCCGAGGA GCGTGAGAGT GTGCAAAAGA AGACATTCAC CAAGTGGGTC 
AATTCGCATC TGTGCCGGGT CAACTGCCGC ATTGCCGATC TCTATGTGGA TATGCGGGAT 
GGCAAGCACC TGATCAAACT GCTGGAGGTC CTCTCCGGCG AGCGACTGCC CAAGCCCACC 
AAGGGCAAGA TGCGTATCCA CTGCCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGCGC 
GAACAGCGCG TTCATCTGGA GAATATTGGC TCCCATGACA TAGTCGATGG CAATGCTTCC 
CTCAATTTGG GCCTGATTTG GACCATCATC CTGCGCTTCC AGATCCAGGA CATCACCATC 
GAGGAGGTGG ACAACAAGGA GACCAAGTCC GCCAAGGACG CCCTGCTGCT CTGGTGCCAG 
ATGAAGACTG CCGGCTATCA CAATGTGAAC GTGCGCAACT TCACCACCTC GTGGCGCGAT 
GGCCTGGCCT TTAATGCCAT CATCCACAAA CACCGTCCGG ATTTGGTTCA GTTCGAGAAA 
CTCTCGAAGA CGAACGCCAT CCACAACCTA AACAATGCCT TCGACGTGGC CGAGGACAAA 
CTGGGCCTGG CCAAGCTCCT TGATGCCGAG GATGTGTTCG TCGAGCATCC GGACGAGAAG 
TCCATCATCA CCTACGTGGT CACCTACTAT CACTACTTTA GCAAACTCAA GCAGGAGACG 
GTGCAGGGCA AGCGTATCGG TAAGGTGGTC GGCATTGCCA TGGAGAACGA CAAAATGGTC 
CACGACTACG AGAACTTCAC AAGCGATCTG CTCAAGTGGA TCGAAACGAC CATCCAGTCG 
CTGGGCGAGC GGGAGTTCGA AAACTCGCTG GCCGGCGTCC AAGGGCAGTT GGCCCAGTTC 
TCCAACTACC GCACCATCGA GAAGCCGCCC AAGTTTGTGG AAAAGGGCAA CCTCGAGGTG 
CTCCTTTTCA CCCTGCAGTC CAAGATGCGG GCCAACAACC AGAAGCCCTA CACACCCAAA 
GAGGGCAAGA TGATTTCGGA CATCAACAAG GCCTGGGAGC GTCTGGAGAA GGCCGAGCAC 
GAACGCGAAT TGGCCCTGCG CGAGGAGCTC ATCCGGCAGG AGAAACTGGA GCAGCTAGCC 
GCCCGCTTCG ACCGCAAGGC GTCCATGCGT GAGACCTGGC TGTCGGAGAA TCAACGTCTG 
GTCAGCCAGG ATAACTTTGG TTTCGATCTG GCGGCCGTTG AGGCGGCGGC CAAGAAGCAC 
GAAGCCATCG AAACCGACAT CTTCGCCTAC GAGGAGCGCG TCCAGGCCGT GGTTGCCGTT 
TGCGATGAAC TGGAATCGGA GCGTTATCAC GATGTGAAGC GCATCCTTCT GCGCAAGGAC 
AACGTTATGC GCTTGTGGAC CTATTTGTTG GAGCTTCTGA GAGCGCGCCG CATGCGCCTG 
GAGATCTCGT TGCAGCTGCA GCAGAACTTC CAGGAGATGC TGTACATCCT CGACAACATG 
GAGGAGATCA AGCAGCTGCT GATGACCGAC GATTATGGCA AGCATCTGAT GGGCGTGGAG 
GATCTGCTGC AGAAGCACTC GCTTGTCGAA GCCGATATCA ATATCCTGGG CGAGCGCGTC 
AAGGTGGTTG TTCAGAACTC GCAGAAGTTC CTGAGCGACG ACCCAGAGTC GTACAAACCG 
TGCGATCCCG AGATCATTGT CTCGCGCGTC CAGCAACTGG AGGATGCTTA CGCAGAGTTG 
GTCCGTTTGG CCGTGGAACG TCGCAGCCGC CTGGAGGAGA GCCGCAAACT CTGGCAGTTC 
TACTGGGACA CCGCCGACGA GGAGAACTGG ATCAAGGAGA AGGAGCAGAT CGTCTCCACC 
GACGAGGTGG GTCACGACCT GACCACCGTC AATCTGATGC TCAGCAAGCA CAAGGCCCTG 
GAGTCGGAGA TCACGTCGCA CGATCCCCAG CTGCAAAATG TGGCCAAGGT CGGTTCGGAG 
CTGATCACCG AGGGTCACTT TGGTGCCGAT CGCATCAAGG ATCGATTGAA GGAGATCCTC 
AACAAGTGGG ACCACCTTCT GGACCTCACC AAGTACCGCC GCCAGCGCTT GGAGAATGCC 
GTCGAGTACT TCCAGCTGTT TGCCGATGCC GACGATGTGG ACAACTGGAT GTTGGACACC 
CTGCGCATTG TTTCCAGCGA GGATGTTGGT CGCGACGAGG CTAACGTACA GTCCCTCCTA 
AAGAAGCACA AGGACGTGGC CGACGAGCTG AAGAATTACG CCGAAGTGAT CGATGCCCTG 
CACAAACAGG CGGAGAGCCT GAAGCTAAAC GAGGCGGAAA AGGCCAATGT GGACAAACGC 
CTGGAGGCGA TCGACAACAG GTACAAGGAG CTTACCGAAC TGGCCAAGCT GCGCAAGCAG 
CGACTGCTCG ACGCCCTCAG CCTGTACAAG CTGATGTCCG AAGCGGATGG CGTGGAGCAA 
TGGATCAAGG AGAAGACCAA GATGCTGGAC ACGATGACTC CTGGCAAGGA CATCGAGGAT 
GTGGAGATCA TGAAGCATCG CTTCGAGGGC TTCGACAAGG AAATGAACGC CAATGCCTCG 
CGCGTGGCCG TCGTTAATCA GCTGGCCCGC CAGCTGCTCC ACGTCGAGCA TCCCAACTCC 
GATGAGATTC TGGAGAGGCA GAACCACCTC AACCAGGAGT GGTCGACACT GCGCGAGAAG 
GCCGAGGCCA AGATGGATGA TCTGAAGTCC GCCCATGGCG TCCAGACCTT CTACATTGAG 
TGCCGCGAGA CCATTTCGTG GATTGAGGAT AAGAAGCGCA TCCTTACCGA GACCGACAGT 
CTGGAGATGG ATCTGACCGG TGTGATGACC CTGCAGCGTC GCCTTAGCGG CATGGACCGC 
GACTTGGCCG CCATTCAGGC CAAGCTCTCG AGCTTGGAGC GCGAAGCCAA CAGCATCGAG 
GATGAGCATC CTGAGGAGGC CAAGATCATC CGCGAGCGCA TTGCCCAGAT CGAGTTGATT 
TGGGAGCAGC TCACCCAGAT GCTCAAGGAG CGCGACTCGA AACTGGAGGA GGCCGGCGAT 
CTGCATCGCT TCTTGCGCGA TCTGGATCAC TTCCAGACTT GGTTGACCAA GACCCAGACA 
GATGTAGCTT CCGAGGACAC TCCCACTTCC CTGCCGGAGG CTGAGAAGCT TCTGAACCAG 
CATCAATCGA TCCGCGAGGA GATCGACAAC TACACTGAGG ACTACAAGAA CATGATGGAG 
TACGGCGAGC GACTGACCTC CGAGGGAAGC ACCTCAGACG ATCCACAGTA CATGTTCTTG 
AGGGAGCGCC TGAATGCCCT GAAGGATGGC TGGGAGGAGC TGCACCAGAT GTGGGAGAAC 
AGACAGGTGC TGTTGTCGCA GAGCTTGGAC CAGCAACTGT TCAACCGCGA TGCCCGCCAG 
ACGGAGGTGT TGCTCAGCCA ACAGGAGCAC TTCCTCAGCA AGGATGATAC CCCCGTCAAC 
CTGGAGCAGG CTGAGAACCA GTTGAAGCGC CACGAGGCCT TCCTCACCAC CATGGAAGCT 
AACGACGACA AGATCAACAC CCTGCTGCAG GTGGCCGACA CCCTGGTGGA AAAGGATCAC 
TTCGATGCGG ACAAGATTGG CAAGCGTGCG GAGAACATTA CCGGACGTCG GGATGACAAC 
CGTCAGCGTG CTCTGGACCA ACACGAGAAG CTGAAGAATC AGGTGAAACT GCACGAGTTC 
CTGCAGGATC TCGAAGAGCT GGCCGAGTGG GTGCAGGAGA AATACGCCAC TTCACAGGAC 
GAGTCGTACA GGAGCGCAAA GACCATCCAC TCCAAGTGGA CGCGCCATCA GGCCTTCGAG 
GCAGAGATTG CCGCCAACAA GGAGCGCCTA TTCGAGGCAG AGAAGTCTGC CCAGGAGCTA 
TCCAAGGAGA AGCCCGAGTT CAAGGACGTC ATCGAGCCCA AGCTTAAGGA GCTGGCGAAG 
CAATTCGACG ACCTGGAGGT GCACACCAAG GAGAAGGGCG CCATGCTGTT CGACGCCAAC 
CGCGAGGTGC TTGTCCAGCA GACATGCGAC GACATCGACT CCTACATCAC CGACCTGGAG 
AAGCAAATTG TCAGCGGAGA CACTGCCAAC GATCTGACAT CCGTGAACAT CCTGATGCAG 
AAGCAGCAGG TTATCCAGAC CCAGATGGCG GTGAAGGCTC GCCAAGTGGA GGAGATCGAC 
AAGCAGACCG AATACCTCCA GAAGACTGTG CCCGAGGAGA AGATCGAACC GATTGTGGTG 
AAGAAGACGG CCGTGCTCGA GCGCTTTGAG AAGATCAAGG CGCCGCTTCT GGAGCGCCAG 
AAGGCGCTGG AGAAGAAGAA GGAGGCCTTC CAGTTCTGTC GCGATGTCGA GGACGAGAAG 
CTCTGGATTG ACGAGAAGCT GCCGGTGGCC AACTCACCGG ATTACGGCAA CTCCCTGTTC 
AACGTACATG TGCTGAAGAA GAAGAACCAG TCGCTGGCCA CAGAGATCGA CAACCACGAG 
CCGCGCATCA ATGCAATCTG CAACAATGGC CGCAAGCTGA TCGACGAGGG CCACGAGGAT 
GCCAAGAAGT TCGAGGCGCT GATCAGCGAT CTGACCCAGA AGTGGCAGGA GCTGAAGGAC 
GCCATCGAGA ACCGACGGAA GCATCTGCTG GAGTCGGAGA AGGTGCAGCA GTACTTCTTC 
GATGCCCAGG AGGCGGAGTC CTGGATGAGC GAGCAGGAGC TCTACATGAT GGTCGAGGAT 
CGCGGCAAGG ACGAGATCAG TGCCCAGAAC CTGATGAAGA AGCACGAAAA CCTGGAGCAA 
TCCGTAGAGG ACTACGCCAA CACCATTCGC CAGCTCGGCG AGGTGGCGCG TCAGTTCAGC 
GGGGATGACA TCTCCTCCGG CGATGCCGTG GCCGTCAAGC AATCGCAGCT GGATAAGCTT 
TACGCTGGTC TCAAGGATCT GGCTGGGGAG AGGAGGGCTC GCCTCAACGA GGCTCTGCAG 
CTGTTCATGC TGAGCCGCGA GGTCGATGAT CTGGAGCAAT GGATTACCGA TCGCGAGGTT 
GTCGCCGGTT CCCAGGAATT GGGCCAGGAC TTTGACCACG TCACTCTGCT GTCGGAGCGC 
TTCAACGAGT TTGCCCGCGA CACAGAGGCC GTTGGCGGCG AGCGAGTGGC CAAGGTGAAC 
GGCATTGCCG ATAATCTTAT CCAGGCCGGA CACTCTGATT CCGCCACGAT TGCCGAATGG 
AAGGACAACC TCAACGAATC GTGGCAGGAT CTGCTGGAGC TGATCGAGAC ACGCACTCAG 
ATGCTGGCCG CTTCTCGGGA GCTGCACAAA TTCTTCCACG ACTGCAAGGA TGTGTTGGGA 
CGCATCCTCG AGAAGCAGCA CGGTGTGTCC GATGAACTGG GACGCGATGC CGGCTCCGTG 
TCGACGCTGC AGCGCAAGCA CTACAACTTC CTGCAGGACC TCATCACCCT ATACTCACAA 
GTCCAGCAGA TCCAGGAGGA ATCCGCCAAG CTGCAGGACG CCTATGCCGG TGATAAGGCC 
AAGGAAATCA CCAATCGCGA ACAGGAGGTG CTCCATGCCT GGGACAACCT CCAGGCTATG 
TGCGATGCCC GCAAACAGAA ACTGGCCGAC ACAGGCGACT TATTCCGCTT CTTCAACATG 
GTGCGCATCC TGATGATCTG GATGGAGGAC CTCGTCCGCC AAATGAACAC ATCAGAGAAG 
CCGCGCGACG TGTCCGGCGT GGAGCTGCTG ATGAACAACC ACCAGAGCCT TAAGGCCGAG 
ATCGACACAC GGGAGGACAA CTTCGGTGCG TGCATCTCGC TGGGCAAGGA GCTGCTCACC 
CGCAACCACT ACGCCTCCGC CGATATCAAG GATCGCCTGA TGACGCTGAG CAATAGCCGC 
AATGCCCTGC TCCGCCGCTG GGAGGAGCGC TGGGAGAATC TCCAGCTGAT CCTGGAGGTG 
TACCAGTTCG CCAGAGACGC TGCCGTGGCC GAGGCCTGGC TGATCGCCCA GGAGCCTTAC 
CTGCTCTCTT CGGAGCTGGG CCACACAATC GACGAGGTGG AGAACCTGAT TAAGAAGCAC 
GAGGCCTTTG AAAAGTCTGC CGCCGCCCAA GAGGAGCGCT TCAGTGCTCT TGAGCGTTTG 
ACAACATTTG AGCTTAAGGA AATGAAGAGG CGCCAGGAGC TGGCGGAAGA GGCTGAGCGA 
CAACGCATCA AGGAGGAGCA GGAGGCCAAG GCCGCCTCGG AGGCGGCGGA ACAGGCTAAA 
CGAGAGGCTG AGCGACGCGA CGACGTGGAT GTTGGTGCCT CGCACGACGA TTCAGAACGA 
GGCGGAACCC CAGGCGCCGG CGAGGGTCAC GAAGGATATG TGACACGAAA GCACGAGTGG 
GACTCGACCA CCAAGAAGGC CTCCAACCGA TCTTGGGATA AGGTATACAT GGCTGCCAAG 
GCCGGACGCA TTTCGTTCTA CAAAGATCAG AAGGGTTATA AGAGTAATCC CGAATTGACC 
TTCCGCGGTG AGCCCAGCTA CGATCTGCAA AACGCTGCCA TCGAAATTGC CAGTGATTAC 
ACCAAGAAGA AGCACGTTCT GCGAGTCAAG CTCGCCAACG GTGCCTTGTT CTTGCTGCAG 
GCCCATGACG ACACCGAGAT GTCCCAGTGG GTGACCTCCC TGAAAGCCCA GAGCGATTCG 
ACAGCGGTGG CCGCCAGCAG ATCCCAGACT CTGCCCGCCA CCTCACAAAA GGACGAACCA 
AAGCGCAGAT CGTTTTTTAC TTTAAAGAAA AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

beta-Spec ( NM_001272732.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259661.1 Drosophila melanogaster beta spectrin, transcript variant C (beta-Spec), mRNA.

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CloneID	OFa03704
Clone ID Related Accession (Same CDS sequence)	NM_001272732.1
Accession Version	NM_001272732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta spectrin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGG ACATTTCGAT TGTTCGCTGG GATCCCAGCC AGGGTCCTGG CAACGAGTAC 
ATCGATGAGT ACGAGTACGA TGGCGGCAAC TCCAGTTCCC GACTGTTCGA GCGGTCCAGG 
ATCAAGGCTC TGGCCGAGGA GCGTGAGAGT GTGCAAAAGA AGACATTCAC CAAGTGGGTC 
AATTCGCATC TGTGCCGGGT CAACTGCCGC ATTGCCGATC TCTATGTGGA TATGCGGGAT 
GGCAAGCACC TGATCAAACT GCTGGAGGTC CTCTCCGGCG AGCGACTGCC CAAGCCCACC 
AAGGGCAAGA TGCGTATCCA CTGCCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGCGC 
GAACAGCGCG TTCATCTGGA GAATATTGGC TCCCATGACA TAGTCGATGG CAATGCTTCC 
CTCAATTTGG GCCTGATTTG GACCATCATC CTGCGCTTCC AGATCCAGGA CATCACCATC 
GAGGAGGTGG ACAACAAGGA GACCAAGTCC GCCAAGGACG CCCTGCTGCT CTGGTGCCAG 
ATGAAGACTG CCGGCTATCA CAATGTGAAC GTGCGCAACT TCACCACCTC GTGGCGCGAT 
GGCCTGGCCT TTAATGCCAT CATCCACAAA CACCGTCCGG ATTTGGTTCA GTTCGAGAAA 
CTCTCGAAGA CGAACGCCAT CCACAACCTA AACAATGCCT TCGACGTGGC CGAGGACAAA 
CTGGGCCTGG CCAAGCTCCT TGATGCCGAG GATGTGTTCG TCGAGCATCC GGACGAGAAG 
TCCATCATCA CCTACGTGGT CACCTACTAT CACTACTTTA GCAAACTCAA GCAGGAGACG 
GTGCAGGGCA AGCGTATCGG TAAGGTGGTC GGCATTGCCA TGGAGAACGA CAAAATGGTC 
CACGACTACG AGAACTTCAC AAGCGATCTG CTCAAGTGGA TCGAAACGAC CATCCAGTCG 
CTGGGCGAGC GGGAGTTCGA AAACTCGCTG GCCGGCGTCC AAGGGCAGTT GGCCCAGTTC 
TCCAACTACC GCACCATCGA GAAGCCGCCC AAGTTTGTGG AAAAGGGCAA CCTCGAGGTG 
CTCCTTTTCA CCCTGCAGTC CAAGATGCGG GCCAACAACC AGAAGCCCTA CACACCCAAA 
GAGGGCAAGA TGATTTCGGA CATCAACAAG GCCTGGGAGC GTCTGGAGAA GGCCGAGCAC 
GAACGCGAAT TGGCCCTGCG CGAGGAGCTC ATCCGGCAGG AGAAACTGGA GCAGCTAGCC 
GCCCGCTTCG ACCGCAAGGC GTCCATGCGT GAGACCTGGC TGTCGGAGAA TCAACGTCTG 
GTCAGCCAGG ATAACTTTGG TTTCGATCTG GCGGCCGTTG AGGCGGCGGC CAAGAAGCAC 
GAAGCCATCG AAACCGACAT CTTCGCCTAC GAGGAGCGCG TCCAGGCCGT GGTTGCCGTT 
TGCGATGAAC TGGAATCGGA GCGTTATCAC GATGTGAAGC GCATCCTTCT GCGCAAGGAC 
AACGTTATGC GCTTGTGGAC CTATTTGTTG GAGCTTCTGA GAGCGCGCCG CATGCGCCTG 
GAGATCTCGT TGCAGCTGCA GCAGAACTTC CAGGAGATGC TGTACATCCT CGACAACATG 
GAGGAGATCA AGCAGCTGCT GATGACCGAC GATTATGGCA AGCATCTGAT GGGCGTGGAG 
GATCTGCTGC AGAAGCACTC GCTTGTCGAA GCCGATATCA ATATCCTGGG CGAGCGCGTC 
AAGGTGGTTG TTCAGAACTC GCAGAAGTTC CTGAGCGACG ACCCAGAGTC GTACAAACCG 
TGCGATCCCG AGATCATTGT CTCGCGCGTC CAGCAACTGG AGGATGCTTA CGCAGAGTTG 
GTCCGTTTGG CCGTGGAACG TCGCAGCCGC CTGGAGGAGA GCCGCAAACT CTGGCAGTTC 
TACTGGGACA CCGCCGACGA GGAGAACTGG ATCAAGGAGA AGGAGCAGAT CGTCTCCACC 
GACGAGGTGG GTCACGACCT GACCACCGTC AATCTGATGC TCAGCAAGCA CAAGGCCCTG 
GAGTCGGAGA TCACGTCGCA CGATCCCCAG CTGCAAAATG TGGCCAAGGT CGGTTCGGAG 
CTGATCACCG AGGGTCACTT TGGTGCCGAT CGCATCAAGG ATCGATTGAA GGAGATCCTC 
AACAAGTGGG ACCACCTTCT GGACCTCACC AAGTACCGCC GCCAGCGCTT GGAGAATGCC 
GTCGAGTACT TCCAGCTGTT TGCCGATGCC GACGATGTGG ACAACTGGAT GTTGGACACC 
CTGCGCATTG TTTCCAGCGA GGATGTTGGT CGCGACGAGG CTAACGTACA GTCCCTCCTA 
AAGAAGCACA AGGACGTGGC CGACGAGCTG AAGAATTACG CCGAAGTGAT CGATGCCCTG 
CACAAACAGG CGGAGAGCCT GAAGCTAAAC GAGGCGGAAA AGGCCAATGT GGACAAACGC 
CTGGAGGCGA TCGACAACAG GTACAAGGAG CTTACCGAAC TGGCCAAGCT GCGCAAGCAG 
CGACTGCTCG ACGCCCTCAG CCTGTACAAG CTGATGTCCG AAGCGGATGG CGTGGAGCAA 
TGGATCAAGG AGAAGACCAA GATGCTGGAC ACGATGACTC CTGGCAAGGA CATCGAGGAT 
GTGGAGATCA TGAAGCATCG CTTCGAGGGC TTCGACAAGG AAATGAACGC CAATGCCTCG 
CGCGTGGCCG TCGTTAATCA GCTGGCCCGC CAGCTGCTCC ACGTCGAGCA TCCCAACTCC 
GATGAGATTC TGGAGAGGCA GAACCACCTC AACCAGGAGT GGTCGACACT GCGCGAGAAG 
GCCGAGGCCA AGATGGATGA TCTGAAGTCC GCCCATGGCG TCCAGACCTT CTACATTGAG 
TGCCGCGAGA CCATTTCGTG GATTGAGGAT AAGAAGCGCA TCCTTACCGA GACCGACAGT 
CTGGAGATGG ATCTGACCGG TGTGATGACC CTGCAGCGTC GCCTTAGCGG CATGGACCGC 
GACTTGGCCG CCATTCAGGC CAAGCTCTCG AGCTTGGAGC GCGAAGCCAA CAGCATCGAG 
GATGAGCATC CTGAGGAGGC CAAGATCATC CGCGAGCGCA TTGCCCAGAT CGAGTTGATT 
TGGGAGCAGC TCACCCAGAT GCTCAAGGAG CGCGACTCGA AACTGGAGGA GGCCGGCGAT 
CTGCATCGCT TCTTGCGCGA TCTGGATCAC TTCCAGACTT GGTTGACCAA GACCCAGACA 
GATGTAGCTT CCGAGGACAC TCCCACTTCC CTGCCGGAGG CTGAGAAGCT TCTGAACCAG 
CATCAATCGA TCCGCGAGGA GATCGACAAC TACACTGAGG ACTACAAGAA CATGATGGAG 
TACGGCGAGC GACTGACCTC CGAGGGAAGC ACCTCAGACG ATCCACAGTA CATGTTCTTG 
AGGGAGCGCC TGAATGCCCT GAAGGATGGC TGGGAGGAGC TGCACCAGAT GTGGGAGAAC 
AGACAGGTGC TGTTGTCGCA GAGCTTGGAC CAGCAACTGT TCAACCGCGA TGCCCGCCAG 
ACGGAGGTGT TGCTCAGCCA ACAGGAGCAC TTCCTCAGCA AGGATGATAC CCCCGTCAAC 
CTGGAGCAGG CTGAGAACCA GTTGAAGCGC CACGAGGCCT TCCTCACCAC CATGGAAGCT 
AACGACGACA AGATCAACAC CCTGCTGCAG GTGGCCGACA CCCTGGTGGA AAAGGATCAC 
TTCGATGCGG ACAAGATTGG CAAGCGTGCG GAGAACATTA CCGGACGTCG GGATGACAAC 
CGTCAGCGTG CTCTGGACCA ACACGAGAAG CTGAAGAATC AGGTGAAACT GCACGAGTTC 
CTGCAGGATC TCGAAGAGCT GGCCGAGTGG GTGCAGGAGA AATACGCCAC TTCACAGGAC 
GAGTCGTACA GGAGCGCAAA GACCATCCAC TCCAAGTGGA CGCGCCATCA GGCCTTCGAG 
GCAGAGATTG CCGCCAACAA GGAGCGCCTA TTCGAGGCAG AGAAGTCTGC CCAGGAGCTA 
TCCAAGGAGA AGCCCGAGTT CAAGGACGTC ATCGAGCCCA AGCTTAAGGA GCTGGCGAAG 
CAATTCGACG ACCTGGAGGT GCACACCAAG GAGAAGGGCG CCATGCTGTT CGACGCCAAC 
CGCGAGGTGC TTGTCCAGCA GACATGCGAC GACATCGACT CCTACATCAC CGACCTGGAG 
AAGCAAATTG TCAGCGGAGA CACTGCCAAC GATCTGACAT CCGTGAACAT CCTGATGCAG 
AAGCAGCAGG TTATCCAGAC CCAGATGGCG GTGAAGGCTC GCCAAGTGGA GGAGATCGAC 
AAGCAGACCG AATACCTCCA GAAGACTGTG CCCGAGGAGA AGATCGAACC GATTGTGGTG 
AAGAAGACGG CCGTGCTCGA GCGCTTTGAG AAGATCAAGG CGCCGCTTCT GGAGCGCCAG 
AAGGCGCTGG AGAAGAAGAA GGAGGCCTTC CAGTTCTGTC GCGATGTCGA GGACGAGAAG 
CTCTGGATTG ACGAGAAGCT GCCGGTGGCC AACTCACCGG ATTACGGCAA CTCCCTGTTC 
AACGTACATG TGCTGAAGAA GAAGAACCAG TCGCTGGCCA CAGAGATCGA CAACCACGAG 
CCGCGCATCA ATGCAATCTG CAACAATGGC CGCAAGCTGA TCGACGAGGG CCACGAGGAT 
GCCAAGAAGT TCGAGGCGCT GATCAGCGAT CTGACCCAGA AGTGGCAGGA GCTGAAGGAC 
GCCATCGAGA ACCGACGGAA GCATCTGCTG GAGTCGGAGA AGGTGCAGCA GTACTTCTTC 
GATGCCCAGG AGGCGGAGTC CTGGATGAGC GAGCAGGAGC TCTACATGAT GGTCGAGGAT 
CGCGGCAAGG ACGAGATCAG TGCCCAGAAC CTGATGAAGA AGCACGAAAA CCTGGAGCAA 
TCCGTAGAGG ACTACGCCAA CACCATTCGC CAGCTCGGCG AGGTGGCGCG TCAGTTCAGC 
GGGGATGACA TCTCCTCCGG CGATGCCGTG GCCGTCAAGC AATCGCAGCT GGATAAGCTT 
TACGCTGGTC TCAAGGATCT GGCTGGGGAG AGGAGGGCTC GCCTCAACGA GGCTCTGCAG 
CTGTTCATGC TGAGCCGCGA GGTCGATGAT CTGGAGCAAT GGATTACCGA TCGCGAGGTT 
GTCGCCGGTT CCCAGGAATT GGGCCAGGAC TTTGACCACG TCACTCTGCT GTCGGAGCGC 
TTCAACGAGT TTGCCCGCGA CACAGAGGCC GTTGGCGGCG AGCGAGTGGC CAAGGTGAAC 
GGCATTGCCG ATAATCTTAT CCAGGCCGGA CACTCTGATT CCGCCACGAT TGCCGAATGG 
AAGGACAACC TCAACGAATC GTGGCAGGAT CTGCTGGAGC TGATCGAGAC ACGCACTCAG 
ATGCTGGCCG CTTCTCGGGA GCTGCACAAA TTCTTCCACG ACTGCAAGGA TGTGTTGGGA 
CGCATCCTCG AGAAGCAGCA CGGTGTGTCC GATGAACTGG GACGCGATGC CGGCTCCGTG 
TCGACGCTGC AGCGCAAGCA CTACAACTTC CTGCAGGACC TCATCACCCT ATACTCACAA 
GTCCAGCAGA TCCAGGAGGA ATCCGCCAAG CTGCAGGACG CCTATGCCGG TGATAAGGCC 
AAGGAAATCA CCAATCGCGA ACAGGAGGTG CTCCATGCCT GGGACAACCT CCAGGCTATG 
TGCGATGCCC GCAAACAGAA ACTGGCCGAC ACAGGCGACT TATTCCGCTT CTTCAACATG 
GTGCGCATCC TGATGATCTG GATGGAGGAC CTCGTCCGCC AAATGAACAC ATCAGAGAAG 
CCGCGCGACG TGTCCGGCGT GGAGCTGCTG ATGAACAACC ACCAGAGCCT TAAGGCCGAG 
ATCGACACAC GGGAGGACAA CTTCGGTGCG TGCATCTCGC TGGGCAAGGA GCTGCTCACC 
CGCAACCACT ACGCCTCCGC CGATATCAAG GATCGCCTGA TGACGCTGAG CAATAGCCGC 
AATGCCCTGC TCCGCCGCTG GGAGGAGCGC TGGGAGAATC TCCAGCTGAT CCTGGAGGTG 
TACCAGTTCG CCAGAGACGC TGCCGTGGCC GAGGCCTGGC TGATCGCCCA GGAGCCTTAC 
CTGCTCTCTT CGGAGCTGGG CCACACAATC GACGAGGTGG AGAACCTGAT TAAGAAGCAC 
GAGGCCTTTG AAAAGTCTGC CGCCGCCCAA GAGGAGCGCT TCAGTGCTCT TGAGCGTTTG 
ACAACATTTG AGCTTAAGGA AATGAAGAGG CGCCAGGAGC TGGCGGAAGA GGCTGAGCGA 
CAACGCATCA AGGAGGAGCA GGAGGCCAAG GCCGCCTCGG AGGCGGCGGA ACAGGCTAAA 
CGAGAGGCTG AGCGACGCGA CGACGTGGAT GTTGGTGCCT CGCACGACGA TTCAGCCGCA 
GTGTCAAGAC CCCAGTCAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259661.1
CDS	362..6805
Translation	MTTDISIVRWDPSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGIAMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAGVQGQLAQFSNYRTIEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAARFDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCDELESERYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQQNFQEMLYILDNMEEIKQLLMTDDYGKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLSDDPESYKPCDPEIIVSRVQQLEDAYAELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQIVSTDEVGHDLTTVNLMLSKHKALESEITSHDPQLQNVAKVGSELITEGHFGADRIKDRLKEILNKWDHLLDLTKYRRQRLENAVEYFQLFADADDVDNWMLDTLRIVSSEDVGRDEANVQSLLKKHKDVADELKNYAEVIDALHKQAESLKLNEAEKANVDKRLEAIDNRYKELTELAKLRKQRLLDALSLYKLMSEADGVEQWIKEKTKMLDTMTPGKDIEDVEIMKHRFEGFDKEMNANASRVAVVNQLARQLLHVEHPNSDEILERQNHLNQEWSTLREKAEAKMDDLKSAHGVQTFYIECRETISWIEDKKRILTETDSLEMDLTGVMTLQRRLSGMDRDLAAIQAKLSSLEREANSIEDEHPEEAKIIRERIAQIELIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQTWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTEDYKNMMEYGERLTSEGSTSDDPQYMFLRERLNALKDGWEELHQMWENRQVLLSQSLDQQLFNRDARQTEVLLSQQEHFLSKDDTPVNLEQAENQLKRHEAFLTTMEANDDKINTLLQVADTLVEKDHFDADKIGKRAENITGRRDDNRQRALDQHEKLKNQVKLHEFLQDLEELAEWVQEKYATSQDESYRSAKTIHSKWTRHQAFEAEIAANKERLFEAEKSAQELSKEKPEFKDVIEPKLKELAKQFDDLEVHTKEKGAMLFDANREVLVQQTCDDIDSYITDLEKQIVSGDTANDLTSVNILMQKQQVIQTQMAVKARQVEEIDKQTEYLQKTVPEEKIEPIVVKKTAVLERFEKIKAPLLERQKALEKKKEAFQFCRDVEDEKLWIDEKLPVANSPDYGNSLFNVHVLKKKNQSLATEIDNHEPRINAICNNGRKLIDEGHEDAKKFEALISDLTQKWQELKDAIENRRKHLLESEKVQQYFFDAQEAESWMSEQELYMMVEDRGKDEISAQNLMKKHENLEQSVEDYANTIRQLGEVARQFSGDDISSGDAVAVKQSQLDKLYAGLKDLAGERRARLNEALQLFMLSREVDDLEQWITDREVVAGSQELGQDFDHVTLLSERFNEFARDTEAVGGERVAKVNGIADNLIQAGHSDSATIAEWKDNLNESWQDLLELIETRTQMLAASRELHKFFHDCKDVLGRILEKQHGVSDELGRDAGSVSTLQRKHYNFLQDLITLYSQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHAWDNLQAMCDARKQKLADTGDLFRFFNMVRILMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFGACISLGKELLTRNHYASADIKDRLMTLSNSRNALLRRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLLSSELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRRQELAEEAERQRIKEEQEAKAASEAAEQAKREAERRDDVDVGASHDDSAAVSRPQSR

Target ORF information:

RefSeq Version	NM_001272732.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta spectrin, transcript variant C (beta-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272732.1

ORF Insert Sequence:

ATGACGACGG ACATTTCGAT TGTTCGCTGG GATCCCAGCC AGGGTCCTGG CAACGAGTAC 
ATCGATGAGT ACGAGTACGA TGGCGGCAAC TCCAGTTCCC GACTGTTCGA GCGGTCCAGG 
ATCAAGGCTC TGGCCGAGGA GCGTGAGAGT GTGCAAAAGA AGACATTCAC CAAGTGGGTC 
AATTCGCATC TGTGCCGGGT CAACTGCCGC ATTGCCGATC TCTATGTGGA TATGCGGGAT 
GGCAAGCACC TGATCAAACT GCTGGAGGTC CTCTCCGGCG AGCGACTGCC CAAGCCCACC 
AAGGGCAAGA TGCGTATCCA CTGCCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGCGC 
GAACAGCGCG TTCATCTGGA GAATATTGGC TCCCATGACA TAGTCGATGG CAATGCTTCC 
CTCAATTTGG GCCTGATTTG GACCATCATC CTGCGCTTCC AGATCCAGGA CATCACCATC 
GAGGAGGTGG ACAACAAGGA GACCAAGTCC GCCAAGGACG CCCTGCTGCT CTGGTGCCAG 
ATGAAGACTG CCGGCTATCA CAATGTGAAC GTGCGCAACT TCACCACCTC GTGGCGCGAT 
GGCCTGGCCT TTAATGCCAT CATCCACAAA CACCGTCCGG ATTTGGTTCA GTTCGAGAAA 
CTCTCGAAGA CGAACGCCAT CCACAACCTA AACAATGCCT TCGACGTGGC CGAGGACAAA 
CTGGGCCTGG CCAAGCTCCT TGATGCCGAG GATGTGTTCG TCGAGCATCC GGACGAGAAG 
TCCATCATCA CCTACGTGGT CACCTACTAT CACTACTTTA GCAAACTCAA GCAGGAGACG 
GTGCAGGGCA AGCGTATCGG TAAGGTGGTC GGCATTGCCA TGGAGAACGA CAAAATGGTC 
CACGACTACG AGAACTTCAC AAGCGATCTG CTCAAGTGGA TCGAAACGAC CATCCAGTCG 
CTGGGCGAGC GGGAGTTCGA AAACTCGCTG GCCGGCGTCC AAGGGCAGTT GGCCCAGTTC 
TCCAACTACC GCACCATCGA GAAGCCGCCC AAGTTTGTGG AAAAGGGCAA CCTCGAGGTG 
CTCCTTTTCA CCCTGCAGTC CAAGATGCGG GCCAACAACC AGAAGCCCTA CACACCCAAA 
GAGGGCAAGA TGATTTCGGA CATCAACAAG GCCTGGGAGC GTCTGGAGAA GGCCGAGCAC 
GAACGCGAAT TGGCCCTGCG CGAGGAGCTC ATCCGGCAGG AGAAACTGGA GCAGCTAGCC 
GCCCGCTTCG ACCGCAAGGC GTCCATGCGT GAGACCTGGC TGTCGGAGAA TCAACGTCTG 
GTCAGCCAGG ATAACTTTGG TTTCGATCTG GCGGCCGTTG AGGCGGCGGC CAAGAAGCAC 
GAAGCCATCG AAACCGACAT CTTCGCCTAC GAGGAGCGCG TCCAGGCCGT GGTTGCCGTT 
TGCGATGAAC TGGAATCGGA GCGTTATCAC GATGTGAAGC GCATCCTTCT GCGCAAGGAC 
AACGTTATGC GCTTGTGGAC CTATTTGTTG GAGCTTCTGA GAGCGCGCCG CATGCGCCTG 
GAGATCTCGT TGCAGCTGCA GCAGAACTTC CAGGAGATGC TGTACATCCT CGACAACATG 
GAGGAGATCA AGCAGCTGCT GATGACCGAC GATTATGGCA AGCATCTGAT GGGCGTGGAG 
GATCTGCTGC AGAAGCACTC GCTTGTCGAA GCCGATATCA ATATCCTGGG CGAGCGCGTC 
AAGGTGGTTG TTCAGAACTC GCAGAAGTTC CTGAGCGACG ACCCAGAGTC GTACAAACCG 
TGCGATCCCG AGATCATTGT CTCGCGCGTC CAGCAACTGG AGGATGCTTA CGCAGAGTTG 
GTCCGTTTGG CCGTGGAACG TCGCAGCCGC CTGGAGGAGA GCCGCAAACT CTGGCAGTTC 
TACTGGGACA CCGCCGACGA GGAGAACTGG ATCAAGGAGA AGGAGCAGAT CGTCTCCACC 
GACGAGGTGG GTCACGACCT GACCACCGTC AATCTGATGC TCAGCAAGCA CAAGGCCCTG 
GAGTCGGAGA TCACGTCGCA CGATCCCCAG CTGCAAAATG TGGCCAAGGT CGGTTCGGAG 
CTGATCACCG AGGGTCACTT TGGTGCCGAT CGCATCAAGG ATCGATTGAA GGAGATCCTC 
AACAAGTGGG ACCACCTTCT GGACCTCACC AAGTACCGCC GCCAGCGCTT GGAGAATGCC 
GTCGAGTACT TCCAGCTGTT TGCCGATGCC GACGATGTGG ACAACTGGAT GTTGGACACC 
CTGCGCATTG TTTCCAGCGA GGATGTTGGT CGCGACGAGG CTAACGTACA GTCCCTCCTA 
AAGAAGCACA AGGACGTGGC CGACGAGCTG AAGAATTACG CCGAAGTGAT CGATGCCCTG 
CACAAACAGG CGGAGAGCCT GAAGCTAAAC GAGGCGGAAA AGGCCAATGT GGACAAACGC 
CTGGAGGCGA TCGACAACAG GTACAAGGAG CTTACCGAAC TGGCCAAGCT GCGCAAGCAG 
CGACTGCTCG ACGCCCTCAG CCTGTACAAG CTGATGTCCG AAGCGGATGG CGTGGAGCAA 
TGGATCAAGG AGAAGACCAA GATGCTGGAC ACGATGACTC CTGGCAAGGA CATCGAGGAT 
GTGGAGATCA TGAAGCATCG CTTCGAGGGC TTCGACAAGG AAATGAACGC CAATGCCTCG 
CGCGTGGCCG TCGTTAATCA GCTGGCCCGC CAGCTGCTCC ACGTCGAGCA TCCCAACTCC 
GATGAGATTC TGGAGAGGCA GAACCACCTC AACCAGGAGT GGTCGACACT GCGCGAGAAG 
GCCGAGGCCA AGATGGATGA TCTGAAGTCC GCCCATGGCG TCCAGACCTT CTACATTGAG 
TGCCGCGAGA CCATTTCGTG GATTGAGGAT AAGAAGCGCA TCCTTACCGA GACCGACAGT 
CTGGAGATGG ATCTGACCGG TGTGATGACC CTGCAGCGTC GCCTTAGCGG CATGGACCGC 
GACTTGGCCG CCATTCAGGC CAAGCTCTCG AGCTTGGAGC GCGAAGCCAA CAGCATCGAG 
GATGAGCATC CTGAGGAGGC CAAGATCATC CGCGAGCGCA TTGCCCAGAT CGAGTTGATT 
TGGGAGCAGC TCACCCAGAT GCTCAAGGAG CGCGACTCGA AACTGGAGGA GGCCGGCGAT 
CTGCATCGCT TCTTGCGCGA TCTGGATCAC TTCCAGACTT GGTTGACCAA GACCCAGACA 
GATGTAGCTT CCGAGGACAC TCCCACTTCC CTGCCGGAGG CTGAGAAGCT TCTGAACCAG 
CATCAATCGA TCCGCGAGGA GATCGACAAC TACACTGAGG ACTACAAGAA CATGATGGAG 
TACGGCGAGC GACTGACCTC CGAGGGAAGC ACCTCAGACG ATCCACAGTA CATGTTCTTG 
AGGGAGCGCC TGAATGCCCT GAAGGATGGC TGGGAGGAGC TGCACCAGAT GTGGGAGAAC 
AGACAGGTGC TGTTGTCGCA GAGCTTGGAC CAGCAACTGT TCAACCGCGA TGCCCGCCAG 
ACGGAGGTGT TGCTCAGCCA ACAGGAGCAC TTCCTCAGCA AGGATGATAC CCCCGTCAAC 
CTGGAGCAGG CTGAGAACCA GTTGAAGCGC CACGAGGCCT TCCTCACCAC CATGGAAGCT 
AACGACGACA AGATCAACAC CCTGCTGCAG GTGGCCGACA CCCTGGTGGA AAAGGATCAC 
TTCGATGCGG ACAAGATTGG CAAGCGTGCG GAGAACATTA CCGGACGTCG GGATGACAAC 
CGTCAGCGTG CTCTGGACCA ACACGAGAAG CTGAAGAATC AGGTGAAACT GCACGAGTTC 
CTGCAGGATC TCGAAGAGCT GGCCGAGTGG GTGCAGGAGA AATACGCCAC TTCACAGGAC 
GAGTCGTACA GGAGCGCAAA GACCATCCAC TCCAAGTGGA CGCGCCATCA GGCCTTCGAG 
GCAGAGATTG CCGCCAACAA GGAGCGCCTA TTCGAGGCAG AGAAGTCTGC CCAGGAGCTA 
TCCAAGGAGA AGCCCGAGTT CAAGGACGTC ATCGAGCCCA AGCTTAAGGA GCTGGCGAAG 
CAATTCGACG ACCTGGAGGT GCACACCAAG GAGAAGGGCG CCATGCTGTT CGACGCCAAC 
CGCGAGGTGC TTGTCCAGCA GACATGCGAC GACATCGACT CCTACATCAC CGACCTGGAG 
AAGCAAATTG TCAGCGGAGA CACTGCCAAC GATCTGACAT CCGTGAACAT CCTGATGCAG 
AAGCAGCAGG TTATCCAGAC CCAGATGGCG GTGAAGGCTC GCCAAGTGGA GGAGATCGAC 
AAGCAGACCG AATACCTCCA GAAGACTGTG CCCGAGGAGA AGATCGAACC GATTGTGGTG 
AAGAAGACGG CCGTGCTCGA GCGCTTTGAG AAGATCAAGG CGCCGCTTCT GGAGCGCCAG 
AAGGCGCTGG AGAAGAAGAA GGAGGCCTTC CAGTTCTGTC GCGATGTCGA GGACGAGAAG 
CTCTGGATTG ACGAGAAGCT GCCGGTGGCC AACTCACCGG ATTACGGCAA CTCCCTGTTC 
AACGTACATG TGCTGAAGAA GAAGAACCAG TCGCTGGCCA CAGAGATCGA CAACCACGAG 
CCGCGCATCA ATGCAATCTG CAACAATGGC CGCAAGCTGA TCGACGAGGG CCACGAGGAT 
GCCAAGAAGT TCGAGGCGCT GATCAGCGAT CTGACCCAGA AGTGGCAGGA GCTGAAGGAC 
GCCATCGAGA ACCGACGGAA GCATCTGCTG GAGTCGGAGA AGGTGCAGCA GTACTTCTTC 
GATGCCCAGG AGGCGGAGTC CTGGATGAGC GAGCAGGAGC TCTACATGAT GGTCGAGGAT 
CGCGGCAAGG ACGAGATCAG TGCCCAGAAC CTGATGAAGA AGCACGAAAA CCTGGAGCAA 
TCCGTAGAGG ACTACGCCAA CACCATTCGC CAGCTCGGCG AGGTGGCGCG TCAGTTCAGC 
GGGGATGACA TCTCCTCCGG CGATGCCGTG GCCGTCAAGC AATCGCAGCT GGATAAGCTT 
TACGCTGGTC TCAAGGATCT GGCTGGGGAG AGGAGGGCTC GCCTCAACGA GGCTCTGCAG 
CTGTTCATGC TGAGCCGCGA GGTCGATGAT CTGGAGCAAT GGATTACCGA TCGCGAGGTT 
GTCGCCGGTT CCCAGGAATT GGGCCAGGAC TTTGACCACG TCACTCTGCT GTCGGAGCGC 
TTCAACGAGT TTGCCCGCGA CACAGAGGCC GTTGGCGGCG AGCGAGTGGC CAAGGTGAAC 
GGCATTGCCG ATAATCTTAT CCAGGCCGGA CACTCTGATT CCGCCACGAT TGCCGAATGG 
AAGGACAACC TCAACGAATC GTGGCAGGAT CTGCTGGAGC TGATCGAGAC ACGCACTCAG 
ATGCTGGCCG CTTCTCGGGA GCTGCACAAA TTCTTCCACG ACTGCAAGGA TGTGTTGGGA 
CGCATCCTCG AGAAGCAGCA CGGTGTGTCC GATGAACTGG GACGCGATGC CGGCTCCGTG 
TCGACGCTGC AGCGCAAGCA CTACAACTTC CTGCAGGACC TCATCACCCT ATACTCACAA 
GTCCAGCAGA TCCAGGAGGA ATCCGCCAAG CTGCAGGACG CCTATGCCGG TGATAAGGCC 
AAGGAAATCA CCAATCGCGA ACAGGAGGTG CTCCATGCCT GGGACAACCT CCAGGCTATG 
TGCGATGCCC GCAAACAGAA ACTGGCCGAC ACAGGCGACT TATTCCGCTT CTTCAACATG 
GTGCGCATCC TGATGATCTG GATGGAGGAC CTCGTCCGCC AAATGAACAC ATCAGAGAAG 
CCGCGCGACG TGTCCGGCGT GGAGCTGCTG ATGAACAACC ACCAGAGCCT TAAGGCCGAG 
ATCGACACAC GGGAGGACAA CTTCGGTGCG TGCATCTCGC TGGGCAAGGA GCTGCTCACC 
CGCAACCACT ACGCCTCCGC CGATATCAAG GATCGCCTGA TGACGCTGAG CAATAGCCGC 
AATGCCCTGC TCCGCCGCTG GGAGGAGCGC TGGGAGAATC TCCAGCTGAT CCTGGAGGTG 
TACCAGTTCG CCAGAGACGC TGCCGTGGCC GAGGCCTGGC TGATCGCCCA GGAGCCTTAC 
CTGCTCTCTT CGGAGCTGGG CCACACAATC GACGAGGTGG AGAACCTGAT TAAGAAGCAC 
GAGGCCTTTG AAAAGTCTGC CGCCGCCCAA GAGGAGCGCT TCAGTGCTCT TGAGCGTTTG 
ACAACATTTG AGCTTAAGGA AATGAAGAGG CGCCAGGAGC TGGCGGAAGA GGCTGAGCGA 
CAACGCATCA AGGAGGAGCA GGAGGCCAAG GCCGCCTCGG AGGCGGCGGA ACAGGCTAAA 
CGAGAGGCTG AGCGACGCGA CGACGTGGAT GTTGGTGCCT CGCACGACGA TTCAGCCGCA 
GTGTCAAGAC CCCAGTCAAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

beta-Spec ( NM_001272731.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259660.1 Drosophila melanogaster beta spectrin, transcript variant B (beta-Spec), mRNA.

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CloneID	OFa03705
Clone ID Related Accession (Same CDS sequence)	NM_001272731.1
Accession Version	NM_001272731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta spectrin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGACGG ACATTTCGAT TGTTCGCTGG GATCCCAGCC AGGGTCCTGG CAACGAGTAC 
ATCGATGAGT ACGAGTACGA TGGCGGCAAC TCCAGTTCCC GACTGTTCGA GCGGTCCAGG 
ATCAAGGCTC TGGCCGAGGA GCGTGAGAGT GTGCAAAAGA AGACATTCAC CAAGTGGGTC 
AATTCGCATC TGTGCCGGGT CAACTGCCGC ATTGCCGATC TCTATGTGGA TATGCGGGAT 
GGCAAGCACC TGATCAAACT GCTGGAGGTC CTCTCCGGCG AGCGACTGCC CAAGCCCACC 
AAGGGCAAGA TGCGTATCCA CTGCCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGCGC 
GAACAGCGCG TTCATCTGGA GAATATTGGC TCCCATGACA TAGTCGATGG CAATGCTTCC 
CTCAATTTGG GCCTGATTTG GACCATCATC CTGCGCTTCC AGATCCAGGA CATCACCATC 
GAGGAGGTGG ACAACAAGGA GACCAAGTCC GCCAAGGACG CCCTGCTGCT CTGGTGCCAG 
ATGAAGACTG CCGGCTATCA CAATGTGAAC GTGCGCAACT TCACCACCTC GTGGCGCGAT 
GGCCTGGCCT TTAATGCCAT CATCCACAAA CACCGTCCGG ATTTGGTTCA GTTCGAGAAA 
CTCTCGAAGA CGAACGCCAT CCACAACCTA AACAATGCCT TCGACGTGGC CGAGGACAAA 
CTGGGCCTGG CCAAGCTCCT TGATGCCGAG GATGTGTTCG TCGAGCATCC GGACGAGAAG 
TCCATCATCA CCTACGTGGT CACCTACTAT CACTACTTTA GCAAACTCAA GCAGGAGACG 
GTGCAGGGCA AGCGTATCGG TAAGGTGGTC GGCATTGCCA TGGAGAACGA CAAAATGGTC 
CACGACTACG AGAACTTCAC AAGCGATCTG CTCAAGTGGA TCGAAACGAC CATCCAGTCG 
CTGGGCGAGC GGGAGTTCGA AAACTCGCTG GCCGGCGTCC AAGGGCAGTT GGCCCAGTTC 
TCCAACTACC GCACCATCGA GAAGCCGCCC AAGTTTGTGG AAAAGGGCAA CCTCGAGGTG 
CTCCTTTTCA CCCTGCAGTC CAAGATGCGG GCCAACAACC AGAAGCCCTA CACACCCAAA 
GAGGGCAAGA TGATTTCGGA CATCAACAAG GCCTGGGAGC GTCTGGAGAA GGCCGAGCAC 
GAACGCGAAT TGGCCCTGCG CGAGGAGCTC ATCCGGCAGG AGAAACTGGA GCAGCTAGCC 
GCCCGCTTCG ACCGCAAGGC GTCCATGCGT GAGACCTGGC TGTCGGAGAA TCAACGTCTG 
GTCAGCCAGG ATAACTTTGG TTTCGATCTG GCGGCCGTTG AGGCGGCGGC CAAGAAGCAC 
GAAGCCATCG AAACCGACAT CTTCGCCTAC GAGGAGCGCG TCCAGGCCGT GGTTGCCGTT 
TGCGATGAAC TGGAATCGGA GCGTTATCAC GATGTGAAGC GCATCCTTCT GCGCAAGGAC 
AACGTTATGC GCTTGTGGAC CTATTTGTTG GAGCTTCTGA GAGCGCGCCG CATGCGCCTG 
GAGATCTCGT TGCAGCTGCA GCAGAACTTC CAGGAGATGC TGTACATCCT CGACAACATG 
GAGGAGATCA AGCAGCTGCT GATGACCGAC GATTATGGCA AGCATCTGAT GGGCGTGGAG 
GATCTGCTGC AGAAGCACTC GCTTGTCGAA GCCGATATCA ATATCCTGGG CGAGCGCGTC 
AAGGTGGTTG TTCAGAACTC GCAGAAGTTC CTGAGCGACG ACCCAGAGTC GTACAAACCG 
TGCGATCCCG AGATCATTGT CTCGCGCGTC CAGCAACTGG AGGATGCTTA CGCAGAGTTG 
GTCCGTTTGG CCGTGGAACG TCGCAGCCGC CTGGAGGAGA GCCGCAAACT CTGGCAGTTC 
TACTGGGACA CCGCCGACGA GGAGAACTGG ATCAAGGAGA AGGAGCAGAT CGTCTCCACC 
GACGAGGTGG GTCACGACCT GACCACCGTC AATCTGATGC TCAGCAAGCA CAAGGCCCTG 
GAGTCGGAGA TCACGTCGCA CGATCCCCAG CTGCAAAATG TGGCCAAGGT CGGTTCGGAG 
CTGATCACCG AGGGTCACTT TGGTGCCGAT CGCATCAAGG ATCGATTGAA GGAGATCCTC 
AACAAGTGGG ACCACCTTCT GGACCTCACC AAGTACCGCC GCCAGCGCTT GGAGAATGCC 
GTCGAGTACT TCCAGCTGTT TGCCGATGCC GACGATGTGG ACAACTGGAT GTTGGACACC 
CTGCGCATTG TTTCCAGCGA GGATGTTGGT CGCGACGAGG CTAACGTACA GTCCCTCCTA 
AAGAAGCACA AGGACGTGGC CGACGAGCTG AAGAATTACG CCGAAGTGAT CGATGCCCTG 
CACAAACAGG CGGAGAGCCT GAAGCTAAAC GAGGCGGAAA AGGCCAATGT GGACAAACGC 
CTGGAGGCGA TCGACAACAG GTACAAGGAG CTTACCGAAC TGGCCAAGCT GCGCAAGCAG 
CGACTGCTCG ACGCCCTCAG CCTGTACAAG CTGATGTCCG AAGCGGATGG CGTGGAGCAA 
TGGATCAAGG AGAAGACCAA GATGCTGGAC ACGATGACTC CTGGCAAGGA CATCGAGGAT 
GTGGAGATCA TGAAGCATCG CTTCGAGGGC TTCGACAAGG AAATGAACGC CAATGCCTCG 
CGCGTGGCCG TCGTTAATCA GCTGGCCCGC CAGCTGCTCC ACGTCGAGCA TCCCAACTCC 
GATGAGATTC TGGAGAGGCA GAACCACCTC AACCAGGAGT GGTCGACACT GCGCGAGAAG 
GCCGAGGCCA AGATGGATGA TCTGAAGTCC GCCCATGGCG TCCAGACCTT CTACATTGAG 
TGCCGCGAGA CCATTTCGTG GATTGAGGAT AAGAAGCGCA TCCTTACCGA GACCGACAGT 
CTGGAGATGG ATCTGACCGG TGTGATGACC CTGCAGCGTC GCCTTAGCGG CATGGACCGC 
GACTTGGCCG CCATTCAGGC CAAGCTCTCG AGCTTGGAGC GCGAAGCCAA CAGCATCGAG 
GATGAGCATC CTGAGGAGGC CAAGATCATC CGCGAGCGCA TTGCCCAGAT CGAGTTGATT 
TGGGAGCAGC TCACCCAGAT GCTCAAGGAG CGCGACTCGA AACTGGAGGA GGCCGGCGAT 
CTGCATCGCT TCTTGCGCGA TCTGGATCAC TTCCAGACTT GGTTGACCAA GACCCAGACA 
GATGTAGCTT CCGAGGACAC TCCCACTTCC CTGCCGGAGG CTGAGAAGCT TCTGAACCAG 
CATCAATCGA TCCGCGAGGA GATCGACAAC TACACTGAGG ACTACAAGAA CATGATGGAG 
TACGGCGAGC GACTGACCTC CGAGGGAAGC ACCTCAGACG ATCCACAGTA CATGTTCTTG 
AGGGAGCGCC TGAATGCCCT GAAGGATGGC TGGGAGGAGC TGCACCAGAT GTGGGAGAAC 
AGACAGGTGC TGTTGTCGCA GAGCTTGGAC CAGCAACTGT TCAACCGCGA TGCCCGCCAG 
ACGGAGGTGT TGCTCAGCCA ACAGGAGCAC TTCCTCAGCA AGGATGATAC CCCCGTCAAC 
CTGGAGCAGG CTGAGAACCA GTTGAAGCGC CACGAGGCCT TCCTCACCAC CATGGAAGCT 
AACGACGACA AGATCAACAC CCTGCTGCAG GTGGCCGACA CCCTGGTGGA AAAGGATCAC 
TTCGATGCGG ACAAGATTGG CAAGCGTGCG GAGAACATTA CCGGACGTCG GGATGACAAC 
CGTCAGCGTG CTCTGGACCA ACACGAGAAG CTGAAGAATC AGGTGAAACT GCACGAGTTC 
CTGCAGGATC TCGAAGAGCT GGCCGAGTGG GTGCAGGAGA AATACGCCAC TTCACAGGAC 
GAGTCGTACA GGAGCGCAAA GACCATCCAC TCCAAGTGGA CGCGCCATCA GGCCTTCGAG 
GCAGAGATTG CCGCCAACAA GGAGCGCCTA TTCGAGGCAG AGAAGTCTGC CCAGGAGCTA 
TCCAAGGAGA AGCCCGAGTT CAAGGACGTC ATCGAGCCCA AGCTTAAGGA GCTGGCGAAG 
CAATTCGACG ACCTGGAGGT GCACACCAAG GAGAAGGGCG CCATGCTGTT CGACGCCAAC 
CGCGAGGTGC TTGTCCAGCA GACATGCGAC GACATCGACT CCTACATCAC CGACCTGGAG 
AAGCAAATTG TCAGCGGAGA CACTGCCAAC GATCTGACAT CCGTGAACAT CCTGATGCAG 
AAGCAGCAGG TTATCCAGAC CCAGATGGCG GTGAAGGCTC GCCAAGTGGA GGAGATCGAC 
AAGCAGACCG AATACCTCCA GAAGACTGTG CCCGAGGAGA AGATCGAACC GATTGTGGTG 
AAGAAGACGG CCGTGCTCGA GCGCTTTGAG AAGATCAAGG CGCCGCTTCT GGAGCGCCAG 
AAGGCGCTGG AGAAGAAGAA GGAGGCCTTC CAGTTCTGTC GCGATGTCGA GGACGAGAAG 
CTCTGGATTG ACGAGAAGCT GCCGGTGGCC AACTCACCGG ATTACGGCAA CTCCCTGTTC 
AACGTACATG TGCTGAAGAA GAAGAACCAG TCGCTGGCCA CAGAGATCGA CAACCACGAG 
CCGCGCATCA ATGCAATCTG CAACAATGGC CGCAAGCTGA TCGACGAGGG CCACGAGGAT 
GCCAAGAAGT TCGAGGCGCT GATCAGCGAT CTGACCCAGA AGTGGCAGGA GCTGAAGGAC 
GCCATCGAGA ACCGACGGAA GCATCTGCTG GAGTCGGAGA AGGTGCAGCA GTACTTCTTC 
GATGCCCAGG AGGCGGAGTC CTGGATGAGC GAGCAGGAGC TCTACATGAT GGTCGAGGAT 
CGCGGCAAGG ACGAGATCAG TGCCCAGAAC CTGATGAAGA AGCACGAAAA CCTGGAGCAA 
TCCGTAGAGG ACTACGCCAA CACCATTCGC CAGCTCGGCG AGGTGGCGCG TCAGTTCAGC 
GGGGATGACA TCTCCTCCGG CGATGCCGTG GCCGTCAAGC AATCGCAGCT GGATAAGCTT 
TACGCTGGTC TCAAGGATCT GGCTGGGGAG AGGAGGGCTC GCCTCAACGA GGCTCTGCAG 
CTGTTCATGC TGAGCCGCGA GGTCGATGAT CTGGAGCAAT GGATTACCGA TCGCGAGGTT 
GTCGCCGGTT CCCAGGAATT GGGCCAGGAC TTTGACCACG TCACTCTGCT GTCGGAGCGC 
TTCAACGAGT TTGCCCGCGA CACAGAGGCC GTTGGCGGCG AGCGAGTGGC CAAGGTGAAC 
GGCATTGCCG ATAATCTTAT CCAGGCCGGA CACTCTGATT CCGCCACGAT TGCCGAATGG 
AAGGACAACC TCAACGAATC GTGGCAGGAT CTGCTGGAGC TGATCGAGAC ACGCACTCAG 
ATGCTGGCCG CTTCTCGGGA GCTGCACAAA TTCTTCCACG ACTGCAAGGA TGTGTTGGGA 
CGCATCCTCG AGAAGCAGCA CGGTGTGTCC GATGAACTGG GACGCGATGC CGGCTCCGTG 
TCGACGCTGC AGCGCAAGCA CTACAACTTC CTGCAGGACC TCATCACCCT ATACTCACAA 
GTCCAGCAGA TCCAGGAGGA ATCCGCCAAG CTGCAGGACG CCTATGCCGG TGATAAGGCC 
AAGGAAATCA CCAATCGCGA ACAGGAGGTG CTCCATGCCT GGGACAACCT CCAGGCTATG 
TGCGATGCCC GCAAACAGAA ACTGGCCGAC ACAGGCGACT TATTCCGCTT CTTCAACATG 
GTGCGCATCC TGATGATCTG GATGGAGGAC CTCGTCCGCC AAATGAACAC ATCAGAGAAG 
CCGCGCGACG TGTCCGGCGT GGAGCTGCTG ATGAACAACC ACCAGAGCCT TAAGGCCGAG 
ATCGACACAC GGGAGGACAA CTTCGGTGCG TGCATCTCGC TGGGCAAGGA GCTGCTCACC 
CGCAACCACT ACGCCTCCGC CGATATCAAG GATCGCCTGA TGACGCTGAG CAATAGCCGC 
AATGCCCTGC TCCGCCGCTG GGAGGAGCGC TGGGAGAATC TCCAGCTGAT CCTGGAGGTG 
TACCAGTTCG CCAGAGACGC TGCCGTGGCC GAGGCCTGGC TGATCGCCCA GGAGCCTTAC 
CTGCTCTCTT CGGAGCTGGG CCACACAATC GACGAGGTGG AGAACCTGAT TAAGAAGCAC 
GAGGCCTTTG AAAAGTCTGC CGCCGCCCAA GAGGAGCGCT TCAGTGCTCT TGAGCGTTTG 
ACAACATTTG AGCTTAAGGA AATGAAGAGG CGCCAGGAGC TGGCGGAAGA GGCTGAGCGA 
CAACGCATCA AGGAGGAGCA GGAGGCCAAG GCCGCCTCGG AGGCGGCGGA ACAGGCTAAA 
CGAGAGGCTG AGCGACGCGA CGACGTGGAT GTTGGTGCCT CGCACGACGA TTCAGCCGCC 
AAAGACACGG CCGTCGAGTT CGAGCGCGTT GTCGAGATCC AAACAGAACG AGGCGGAACC 
CCAGGCGCCG GCGAGGGTCA CGAAGGATAT GTGACACGAA AGCACGAGTG GGACTCGACC 
ACCAAGAAGG CCTCCAACCG ATCTTGGGAT AAGGTATACA TGGCTGCCAA GGCCGGACGC 
ATTTCGTTCT ACAAAGATCA GAAGGGTTAT AAGAGTAATC CCGAATTGAC CTTCCGCGGT 
GAGCCCAGCT ACGATCTGCA AAACGCTGCC ATCGAAATTG CCAGTGATTA CACCAAGAAG 
AAGCACGTTC TGCGAGTCAA GCTCGCCAAC GGTGCCTTGT TCTTGCTGCA GGCCCATGAC 
GACACCGAGA TGTCCCAGTG GGTGACCTCC CTGAAAGCCC AGAGCGATTC GACAGCGGTG 
GCCGCCAGCA GATCCCAGAC TCTGCCCGCC ACCTCACAAA AGGACGAACC AAAGCGCAGA 
TCGTTTTTTA CTTTAAAGAA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259660.1
CDS	358..7284
Translation	MTTDISIVRWDPSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYHYFSKLKQETVQGKRIGKVVGIAMENDKMVHDYENFTSDLLKWIETTIQSLGEREFENSLAGVQGQLAQFSNYRTIEKPPKFVEKGNLEVLLFTLQSKMRANNQKPYTPKEGKMISDINKAWERLEKAEHERELALREELIRQEKLEQLAARFDRKASMRETWLSENQRLVSQDNFGFDLAAVEAAAKKHEAIETDIFAYEERVQAVVAVCDELESERYHDVKRILLRKDNVMRLWTYLLELLRARRMRLEISLQLQQNFQEMLYILDNMEEIKQLLMTDDYGKHLMGVEDLLQKHSLVEADINILGERVKVVVQNSQKFLSDDPESYKPCDPEIIVSRVQQLEDAYAELVRLAVERRSRLEESRKLWQFYWDTADEENWIKEKEQIVSTDEVGHDLTTVNLMLSKHKALESEITSHDPQLQNVAKVGSELITEGHFGADRIKDRLKEILNKWDHLLDLTKYRRQRLENAVEYFQLFADADDVDNWMLDTLRIVSSEDVGRDEANVQSLLKKHKDVADELKNYAEVIDALHKQAESLKLNEAEKANVDKRLEAIDNRYKELTELAKLRKQRLLDALSLYKLMSEADGVEQWIKEKTKMLDTMTPGKDIEDVEIMKHRFEGFDKEMNANASRVAVVNQLARQLLHVEHPNSDEILERQNHLNQEWSTLREKAEAKMDDLKSAHGVQTFYIECRETISWIEDKKRILTETDSLEMDLTGVMTLQRRLSGMDRDLAAIQAKLSSLEREANSIEDEHPEEAKIIRERIAQIELIWEQLTQMLKERDSKLEEAGDLHRFLRDLDHFQTWLTKTQTDVASEDTPTSLPEAEKLLNQHQSIREEIDNYTEDYKNMMEYGERLTSEGSTSDDPQYMFLRERLNALKDGWEELHQMWENRQVLLSQSLDQQLFNRDARQTEVLLSQQEHFLSKDDTPVNLEQAENQLKRHEAFLTTMEANDDKINTLLQVADTLVEKDHFDADKIGKRAENITGRRDDNRQRALDQHEKLKNQVKLHEFLQDLEELAEWVQEKYATSQDESYRSAKTIHSKWTRHQAFEAEIAANKERLFEAEKSAQELSKEKPEFKDVIEPKLKELAKQFDDLEVHTKEKGAMLFDANREVLVQQTCDDIDSYITDLEKQIVSGDTANDLTSVNILMQKQQVIQTQMAVKARQVEEIDKQTEYLQKTVPEEKIEPIVVKKTAVLERFEKIKAPLLERQKALEKKKEAFQFCRDVEDEKLWIDEKLPVANSPDYGNSLFNVHVLKKKNQSLATEIDNHEPRINAICNNGRKLIDEGHEDAKKFEALISDLTQKWQELKDAIENRRKHLLESEKVQQYFFDAQEAESWMSEQELYMMVEDRGKDEISAQNLMKKHENLEQSVEDYANTIRQLGEVARQFSGDDISSGDAVAVKQSQLDKLYAGLKDLAGERRARLNEALQLFMLSREVDDLEQWITDREVVAGSQELGQDFDHVTLLSERFNEFARDTEAVGGERVAKVNGIADNLIQAGHSDSATIAEWKDNLNESWQDLLELIETRTQMLAASRELHKFFHDCKDVLGRILEKQHGVSDELGRDAGSVSTLQRKHYNFLQDLITLYSQVQQIQEESAKLQDAYAGDKAKEITNREQEVLHAWDNLQAMCDARKQKLADTGDLFRFFNMVRILMIWMEDLVRQMNTSEKPRDVSGVELLMNNHQSLKAEIDTREDNFGACISLGKELLTRNHYASADIKDRLMTLSNSRNALLRRWEERWENLQLILEVYQFARDAAVAEAWLIAQEPYLLSSELGHTIDEVENLIKKHEAFEKSAAAQEERFSALERLTTFELKEMKRRQELAEEAERQRIKEEQEAKAASEAAEQAKREAERRDDVDVGASHDDSAAKDTAVEFERVVEIQTERGGTPGAGEGHEGYVTRKHEWDSTTKKASNRSWDKVYMAAKAGRISFYKDQKGYKSNPELTFRGEPSYDLQNAAIEIASDYTKKKHVLRVKLANGALFLLQAHDDTEMSQWVTSLKAQSDSTAVAASRSQTLPATSQKDEPKRRSFFTLKKK

Target ORF information:

RefSeq Version	NM_001272731.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta spectrin, transcript variant B (beta-Spec), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272731.1

ORF Insert Sequence:

ATGACGACGG ACATTTCGAT TGTTCGCTGG GATCCCAGCC AGGGTCCTGG CAACGAGTAC 
ATCGATGAGT ACGAGTACGA TGGCGGCAAC TCCAGTTCCC GACTGTTCGA GCGGTCCAGG 
ATCAAGGCTC TGGCCGAGGA GCGTGAGAGT GTGCAAAAGA AGACATTCAC CAAGTGGGTC 
AATTCGCATC TGTGCCGGGT CAACTGCCGC ATTGCCGATC TCTATGTGGA TATGCGGGAT 
GGCAAGCACC TGATCAAACT GCTGGAGGTC CTCTCCGGCG AGCGACTGCC CAAGCCCACC 
AAGGGCAAGA TGCGTATCCA CTGCCTGGAG AACGTGGACA AGGCTCTGCA GTTCCTGCGC 
GAACAGCGCG TTCATCTGGA GAATATTGGC TCCCATGACA TAGTCGATGG CAATGCTTCC 
CTCAATTTGG GCCTGATTTG GACCATCATC CTGCGCTTCC AGATCCAGGA CATCACCATC 
GAGGAGGTGG ACAACAAGGA GACCAAGTCC GCCAAGGACG CCCTGCTGCT CTGGTGCCAG 
ATGAAGACTG CCGGCTATCA CAATGTGAAC GTGCGCAACT TCACCACCTC GTGGCGCGAT 
GGCCTGGCCT TTAATGCCAT CATCCACAAA CACCGTCCGG ATTTGGTTCA GTTCGAGAAA 
CTCTCGAAGA CGAACGCCAT CCACAACCTA AACAATGCCT TCGACGTGGC CGAGGACAAA 
CTGGGCCTGG CCAAGCTCCT TGATGCCGAG GATGTGTTCG TCGAGCATCC GGACGAGAAG 
TCCATCATCA CCTACGTGGT CACCTACTAT CACTACTTTA GCAAACTCAA GCAGGAGACG 
GTGCAGGGCA AGCGTATCGG TAAGGTGGTC GGCATTGCCA TGGAGAACGA CAAAATGGTC 
CACGACTACG AGAACTTCAC AAGCGATCTG CTCAAGTGGA TCGAAACGAC CATCCAGTCG 
CTGGGCGAGC GGGAGTTCGA AAACTCGCTG GCCGGCGTCC AAGGGCAGTT GGCCCAGTTC 
TCCAACTACC GCACCATCGA GAAGCCGCCC AAGTTTGTGG AAAAGGGCAA CCTCGAGGTG 
CTCCTTTTCA CCCTGCAGTC CAAGATGCGG GCCAACAACC AGAAGCCCTA CACACCCAAA 
GAGGGCAAGA TGATTTCGGA CATCAACAAG GCCTGGGAGC GTCTGGAGAA GGCCGAGCAC 
GAACGCGAAT TGGCCCTGCG CGAGGAGCTC ATCCGGCAGG AGAAACTGGA GCAGCTAGCC 
GCCCGCTTCG ACCGCAAGGC GTCCATGCGT GAGACCTGGC TGTCGGAGAA TCAACGTCTG 
GTCAGCCAGG ATAACTTTGG TTTCGATCTG GCGGCCGTTG AGGCGGCGGC CAAGAAGCAC 
GAAGCCATCG AAACCGACAT CTTCGCCTAC GAGGAGCGCG TCCAGGCCGT GGTTGCCGTT 
TGCGATGAAC TGGAATCGGA GCGTTATCAC GATGTGAAGC GCATCCTTCT GCGCAAGGAC 
AACGTTATGC GCTTGTGGAC CTATTTGTTG GAGCTTCTGA GAGCGCGCCG CATGCGCCTG 
GAGATCTCGT TGCAGCTGCA GCAGAACTTC CAGGAGATGC TGTACATCCT CGACAACATG 
GAGGAGATCA AGCAGCTGCT GATGACCGAC GATTATGGCA AGCATCTGAT GGGCGTGGAG 
GATCTGCTGC AGAAGCACTC GCTTGTCGAA GCCGATATCA ATATCCTGGG CGAGCGCGTC 
AAGGTGGTTG TTCAGAACTC GCAGAAGTTC CTGAGCGACG ACCCAGAGTC GTACAAACCG 
TGCGATCCCG AGATCATTGT CTCGCGCGTC CAGCAACTGG AGGATGCTTA CGCAGAGTTG 
GTCCGTTTGG CCGTGGAACG TCGCAGCCGC CTGGAGGAGA GCCGCAAACT CTGGCAGTTC 
TACTGGGACA CCGCCGACGA GGAGAACTGG ATCAAGGAGA AGGAGCAGAT CGTCTCCACC 
GACGAGGTGG GTCACGACCT GACCACCGTC AATCTGATGC TCAGCAAGCA CAAGGCCCTG 
GAGTCGGAGA TCACGTCGCA CGATCCCCAG CTGCAAAATG TGGCCAAGGT CGGTTCGGAG 
CTGATCACCG AGGGTCACTT TGGTGCCGAT CGCATCAAGG ATCGATTGAA GGAGATCCTC 
AACAAGTGGG ACCACCTTCT GGACCTCACC AAGTACCGCC GCCAGCGCTT GGAGAATGCC 
GTCGAGTACT TCCAGCTGTT TGCCGATGCC GACGATGTGG ACAACTGGAT GTTGGACACC 
CTGCGCATTG TTTCCAGCGA GGATGTTGGT CGCGACGAGG CTAACGTACA GTCCCTCCTA 
AAGAAGCACA AGGACGTGGC CGACGAGCTG AAGAATTACG CCGAAGTGAT CGATGCCCTG 
CACAAACAGG CGGAGAGCCT GAAGCTAAAC GAGGCGGAAA AGGCCAATGT GGACAAACGC 
CTGGAGGCGA TCGACAACAG GTACAAGGAG CTTACCGAAC TGGCCAAGCT GCGCAAGCAG 
CGACTGCTCG ACGCCCTCAG CCTGTACAAG CTGATGTCCG AAGCGGATGG CGTGGAGCAA 
TGGATCAAGG AGAAGACCAA GATGCTGGAC ACGATGACTC CTGGCAAGGA CATCGAGGAT 
GTGGAGATCA TGAAGCATCG CTTCGAGGGC TTCGACAAGG AAATGAACGC CAATGCCTCG 
CGCGTGGCCG TCGTTAATCA GCTGGCCCGC CAGCTGCTCC ACGTCGAGCA TCCCAACTCC 
GATGAGATTC TGGAGAGGCA GAACCACCTC AACCAGGAGT GGTCGACACT GCGCGAGAAG 
GCCGAGGCCA AGATGGATGA TCTGAAGTCC GCCCATGGCG TCCAGACCTT CTACATTGAG 
TGCCGCGAGA CCATTTCGTG GATTGAGGAT AAGAAGCGCA TCCTTACCGA GACCGACAGT 
CTGGAGATGG ATCTGACCGG TGTGATGACC CTGCAGCGTC GCCTTAGCGG CATGGACCGC 
GACTTGGCCG CCATTCAGGC CAAGCTCTCG AGCTTGGAGC GCGAAGCCAA CAGCATCGAG 
GATGAGCATC CTGAGGAGGC CAAGATCATC CGCGAGCGCA TTGCCCAGAT CGAGTTGATT 
TGGGAGCAGC TCACCCAGAT GCTCAAGGAG CGCGACTCGA AACTGGAGGA GGCCGGCGAT 
CTGCATCGCT TCTTGCGCGA TCTGGATCAC TTCCAGACTT GGTTGACCAA GACCCAGACA 
GATGTAGCTT CCGAGGACAC TCCCACTTCC CTGCCGGAGG CTGAGAAGCT TCTGAACCAG 
CATCAATCGA TCCGCGAGGA GATCGACAAC TACACTGAGG ACTACAAGAA CATGATGGAG 
TACGGCGAGC GACTGACCTC CGAGGGAAGC ACCTCAGACG ATCCACAGTA CATGTTCTTG 
AGGGAGCGCC TGAATGCCCT GAAGGATGGC TGGGAGGAGC TGCACCAGAT GTGGGAGAAC 
AGACAGGTGC TGTTGTCGCA GAGCTTGGAC CAGCAACTGT TCAACCGCGA TGCCCGCCAG 
ACGGAGGTGT TGCTCAGCCA ACAGGAGCAC TTCCTCAGCA AGGATGATAC CCCCGTCAAC 
CTGGAGCAGG CTGAGAACCA GTTGAAGCGC CACGAGGCCT TCCTCACCAC CATGGAAGCT 
AACGACGACA AGATCAACAC CCTGCTGCAG GTGGCCGACA CCCTGGTGGA AAAGGATCAC 
TTCGATGCGG ACAAGATTGG CAAGCGTGCG GAGAACATTA CCGGACGTCG GGATGACAAC 
CGTCAGCGTG CTCTGGACCA ACACGAGAAG CTGAAGAATC AGGTGAAACT GCACGAGTTC 
CTGCAGGATC TCGAAGAGCT GGCCGAGTGG GTGCAGGAGA AATACGCCAC TTCACAGGAC 
GAGTCGTACA GGAGCGCAAA GACCATCCAC TCCAAGTGGA CGCGCCATCA GGCCTTCGAG 
GCAGAGATTG CCGCCAACAA GGAGCGCCTA TTCGAGGCAG AGAAGTCTGC CCAGGAGCTA 
TCCAAGGAGA AGCCCGAGTT CAAGGACGTC ATCGAGCCCA AGCTTAAGGA GCTGGCGAAG 
CAATTCGACG ACCTGGAGGT GCACACCAAG GAGAAGGGCG CCATGCTGTT CGACGCCAAC 
CGCGAGGTGC TTGTCCAGCA GACATGCGAC GACATCGACT CCTACATCAC CGACCTGGAG 
AAGCAAATTG TCAGCGGAGA CACTGCCAAC GATCTGACAT CCGTGAACAT CCTGATGCAG 
AAGCAGCAGG TTATCCAGAC CCAGATGGCG GTGAAGGCTC GCCAAGTGGA GGAGATCGAC 
AAGCAGACCG AATACCTCCA GAAGACTGTG CCCGAGGAGA AGATCGAACC GATTGTGGTG 
AAGAAGACGG CCGTGCTCGA GCGCTTTGAG AAGATCAAGG CGCCGCTTCT GGAGCGCCAG 
AAGGCGCTGG AGAAGAAGAA GGAGGCCTTC CAGTTCTGTC GCGATGTCGA GGACGAGAAG 
CTCTGGATTG ACGAGAAGCT GCCGGTGGCC AACTCACCGG ATTACGGCAA CTCCCTGTTC 
AACGTACATG TGCTGAAGAA GAAGAACCAG TCGCTGGCCA CAGAGATCGA CAACCACGAG 
CCGCGCATCA ATGCAATCTG CAACAATGGC CGCAAGCTGA TCGACGAGGG CCACGAGGAT 
GCCAAGAAGT TCGAGGCGCT GATCAGCGAT CTGACCCAGA AGTGGCAGGA GCTGAAGGAC 
GCCATCGAGA ACCGACGGAA GCATCTGCTG GAGTCGGAGA AGGTGCAGCA GTACTTCTTC 
GATGCCCAGG AGGCGGAGTC CTGGATGAGC GAGCAGGAGC TCTACATGAT GGTCGAGGAT 
CGCGGCAAGG ACGAGATCAG TGCCCAGAAC CTGATGAAGA AGCACGAAAA CCTGGAGCAA 
TCCGTAGAGG ACTACGCCAA CACCATTCGC CAGCTCGGCG AGGTGGCGCG TCAGTTCAGC 
GGGGATGACA TCTCCTCCGG CGATGCCGTG GCCGTCAAGC AATCGCAGCT GGATAAGCTT 
TACGCTGGTC TCAAGGATCT GGCTGGGGAG AGGAGGGCTC GCCTCAACGA GGCTCTGCAG 
CTGTTCATGC TGAGCCGCGA GGTCGATGAT CTGGAGCAAT GGATTACCGA TCGCGAGGTT 
GTCGCCGGTT CCCAGGAATT GGGCCAGGAC TTTGACCACG TCACTCTGCT GTCGGAGCGC 
TTCAACGAGT TTGCCCGCGA CACAGAGGCC GTTGGCGGCG AGCGAGTGGC CAAGGTGAAC 
GGCATTGCCG ATAATCTTAT CCAGGCCGGA CACTCTGATT CCGCCACGAT TGCCGAATGG 
AAGGACAACC TCAACGAATC GTGGCAGGAT CTGCTGGAGC TGATCGAGAC ACGCACTCAG 
ATGCTGGCCG CTTCTCGGGA GCTGCACAAA TTCTTCCACG ACTGCAAGGA TGTGTTGGGA 
CGCATCCTCG AGAAGCAGCA CGGTGTGTCC GATGAACTGG GACGCGATGC CGGCTCCGTG 
TCGACGCTGC AGCGCAAGCA CTACAACTTC CTGCAGGACC TCATCACCCT ATACTCACAA 
GTCCAGCAGA TCCAGGAGGA ATCCGCCAAG CTGCAGGACG CCTATGCCGG TGATAAGGCC 
AAGGAAATCA CCAATCGCGA ACAGGAGGTG CTCCATGCCT GGGACAACCT CCAGGCTATG 
TGCGATGCCC GCAAACAGAA ACTGGCCGAC ACAGGCGACT TATTCCGCTT CTTCAACATG 
GTGCGCATCC TGATGATCTG GATGGAGGAC CTCGTCCGCC AAATGAACAC ATCAGAGAAG 
CCGCGCGACG TGTCCGGCGT GGAGCTGCTG ATGAACAACC ACCAGAGCCT TAAGGCCGAG 
ATCGACACAC GGGAGGACAA CTTCGGTGCG TGCATCTCGC TGGGCAAGGA GCTGCTCACC 
CGCAACCACT ACGCCTCCGC CGATATCAAG GATCGCCTGA TGACGCTGAG CAATAGCCGC 
AATGCCCTGC TCCGCCGCTG GGAGGAGCGC TGGGAGAATC TCCAGCTGAT CCTGGAGGTG 
TACCAGTTCG CCAGAGACGC TGCCGTGGCC GAGGCCTGGC TGATCGCCCA GGAGCCTTAC 
CTGCTCTCTT CGGAGCTGGG CCACACAATC GACGAGGTGG AGAACCTGAT TAAGAAGCAC 
GAGGCCTTTG AAAAGTCTGC CGCCGCCCAA GAGGAGCGCT TCAGTGCTCT TGAGCGTTTG 
ACAACATTTG AGCTTAAGGA AATGAAGAGG CGCCAGGAGC TGGCGGAAGA GGCTGAGCGA 
CAACGCATCA AGGAGGAGCA GGAGGCCAAG GCCGCCTCGG AGGCGGCGGA ACAGGCTAAA 
CGAGAGGCTG AGCGACGCGA CGACGTGGAT GTTGGTGCCT CGCACGACGA TTCAGCCGCC 
AAAGACACGG CCGTCGAGTT CGAGCGCGTT GTCGAGATCC AAACAGAACG AGGCGGAACC 
CCAGGCGCCG GCGAGGGTCA CGAAGGATAT GTGACACGAA AGCACGAGTG GGACTCGACC 
ACCAAGAAGG CCTCCAACCG ATCTTGGGAT AAGGTATACA TGGCTGCCAA GGCCGGACGC 
ATTTCGTTCT ACAAAGATCA GAAGGGTTAT AAGAGTAATC CCGAATTGAC CTTCCGCGGT 
GAGCCCAGCT ACGATCTGCA AAACGCTGCC ATCGAAATTG CCAGTGATTA CACCAAGAAG 
AAGCACGTTC TGCGAGTCAA GCTCGCCAAC GGTGCCTTGT TCTTGCTGCA GGCCCATGAC 
GACACCGAGA TGTCCCAGTG GGTGACCTCC CTGAAAGCCC AGAGCGATTC GACAGCGGTG 
GCCGCCAGCA GATCCCAGAC TCTGCCCGCC ACCTCACAAA AGGACGAACC AAAGCGCAGA 
TCGTTTTTTA CTTTAAAGAA AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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beta-Spec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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