SPTBN1 cDNA ORF clone, Gallus gallus(chicken)

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa33037 XM_015283351.2
Latest version!
Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa33038 XM_015283353.1
Latest version!
Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa00878 NM_001199425.2
Latest version!
Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OGa00878 XM_015283350.2
Latest version!
Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
View Old Accession Versions >>
OGa00878 XM_015283350.1 Gallus gallus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OGa33037 XM_015283351.1 Gallus gallus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OGa00878 NM_001199425.1 Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa33037
    Clone ID Related Accession (Same CDS sequence) XM_015283351.1 , XM_015283351.2
    Accession Version XM_015283351.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTAC AAAAATCAAC CAGTATGCCT GGGCCGTTGT CCCCAGGATA CGCAGCTCAG 
    GTGCCCTATA ACTATAATCA GTTAGAAGGA AGATTCAAGC AGTTACAAGA TGAACGTGAG
    GCAGTTCAGA AGAAGACTTT CACCAAGTGG GTTAATTCCC ACTTGGCACG TGTGTCATGC
    AGAATTACAG ATTTGTACAC CGACCTTCGC GATGGAAGGA TGCTCATCAA ACTCCTGGAA
    GTTCTTTCAG GAGAGAGACT TCCCAAACCA ACTAAGGGAA GGATGCGCAT CCATTGTTTG
    GAGAATGTTG ATAAAGCCCT GCAGTTCCTG AAGGAGCAAC GAGTGCACCT GGAAAACATG
    GGATCTCATG ATATTGTGGA TGGGAATCAC AGGCTGACTC TTGGCCTTAT TTGGACTATT
    ATCCTGAGAT TCCAGATCCA AGATATCAGT GTTGAAACTG AGGACAACAA GGAGAAGAAG
    TCTGCTAAGG ATGCACTGCT TTTGTGGTGC CAGATGAAGA CAGCTGGTTA CCCCAACGTC
    AACATTCACA ACTTTACAAC AAGCTGGAGG GATGGAATGG CTTTCAATGC ATTGATACAC
    AAGCACAGGC CTGATCTGAT TGATTTTGAC AAGCTGAAGA AATCAAATGC ACACTACAAT
    CTGCAGAATG CTTTTAACCT TGCTGAGCAA CACCTTGGTC TTACTAAGCT CCTGGACCCT
    GAGGATATCA GTGTTGATCA TCCCGATGAA AAGTCAATCA TCACCTATGT GGTGACATAT
    TACCACTACT TCTCTAAAAT GAAAGCTTTA GCTGTTGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGACAATG CCATTGAAAC AGAGAAAATG ATTGAAAAAT ATGAATCGCT GGCTTCTGAT
    CTTCTGGAGT GGATTGAGCA AACCATTATC ATCCTCAACA ACCGCAAATT TGCCAACTCG
    CTAGTTGGTG TGCAACAGCA GCTGCAGGCA TTCAACACCT ACCGCACAGT AGAGAAACCA
    CCCAAATTTA CAGAAAAAGG AAACCTGGAA GTGCTGCTTT TTACCATCCA AAGCAAAATG
    AGAGCCAACA ATCAGAAAGT ATACATGCCA AGGGAGGGCA AACTCATCTC TGACATAAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCTGAA CATGAAAGAG AACTGGCACT GCGAAATGAG
    CTCATCAGAC AAGAGAAACT GGAACAACTT GCACGCCGAT TTGATCGCAA GGCAGCTATG
    AGAGAAACTT GGCTGAGTGA AAATCAACGT CTGGTTTCTC AGGATAACTT TGGCTTTGAC
    CTTCCAGCAG TAGAGGCTGC AACGAAAAAG CACGAGGCCA TTGAAACAGA CATTGCAGCA
    TATGAAGAGC GAGTCCAGGC TGTGGTTGCA GTTGCAAAGG AGCTTGAGAC TGAAAATTAT
    CATGATATCA AACGTATCAC TGCAAGAAAG GACAATGTTA TACGCCTGTG GGAGTACCTC
    CTGGAGCTAC TCAGGGCACG AAGACAACGG CTGGAAATGA ATCTTGGCCT ACAAAAGATT
    TTCCAGGAAA TGCTATACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TCTGCTGTCA
    CAAGACTATG GCAAACATTT GTTAGGAGTC GAAGACCTGT TGCAGAAACA TGCTCTAGTG
    GAAGCTGACA TTGCTATTCA GGCTGAGAGA GTGAGAGGGG TCAATGCATC TGCTCAGAAG
    TTTGCCACTG ATGGAGAAGG TTACAAGCCA TGTGACCCAC AGGTTATTAG GGATCGCGTC
    GCTCACATGG AGTTCTGTTA CCAAGAGCTG TGCCAGCTTT CAGCTGAGCG CCGGGCCCGC
    TTGGAGGAGT CCCGGCGCCT CTGGAAATTC TTCTGGGAAA TGGCTGAAGA AGAGGGTTGG
    ATCAGGGAGA AGGAGCAGAT CCTTTCATCT GATGACTACG GGAAGGACCT GACCAGCGTC
    GTCCGTCTCA TGAGTAAACA TAAGGCATTT GAAGATGAAA TGAGTGGTCG CAGTGGCCAC
    TTTCAGCAGG CGATAAAAGA AGGTGAAGAC ATGATCGCGG AAGAGAATTT TGGGTCTGAG
    AAAATCAGAG AAAGAATTAA GGATATTCGG GAGCAGTGGG CTAACTTGGA ACAGTTATCT
    GCCATTAGGA AGAAACGTCT GGAGGAAGCC TCTCTCCTTC ACCAGTTCCA GGCTGATGCT
    GATGACATTG ATGCATGGAT GTTGGACATC CTCAAAATTG TCTCCAGCAA TGATGTTGGC
    CATGACGAAT ATTCCACTCA GTCCTTGGTC AAGAAACACA AGGATGTTGC TGAAGAGATT
    GCAAGCTATC GGCCAACCAT TGACTCGCTT CATGAACAGG CAAAGGCTCT ACCACAGGAG
    CATGCCGGGT CTCCAGATGT GCAAGGCAGG TTGTCTGGAA TAGAAGAAAG ATACAAAGAG
    GTTGCTGAGC TAACTCGGCT GCGTAAACAG GCTTTGCAGG ATACCCTTGC TCTTTATAAG
    ATGTTCAGTG AGGCAGATGC TTGTGAGCTT TGGATTGATG AGAAGGAGAA GTGGCTGAAT
    AATATGCAGA TTCCAGAGAA GCTGGAGGAC CTGGAAGTGA TCCAGCACAG ATTTGAAAGC
    TTAGAACCAG AAATGAACAA TCAGGCTTCC CGTGTTGCTG TAGTGAACCA AATTGCAAGA
    CAGCTAATGC ATAGTGGCCA TCCAAGTGAG AAGGAGATAA AAGCACAGCA AGATAAACTC
    AACACAAGAT GGAGCCAGTT CAGAGAACTG GTAGACAGGA AGAAAGATGC CCTGATCTCT
    GCCTTAAGTA TCCAGAATTA CCACCTTGAA TGTAATGAAA CCAAGTCCTG GATCAGGGAG
    AAAACCAAAG TGATTGAATC CACACAAGAT TTGGGTAACG ATCTTGCTGG AGTGATGGCT
    CTGCAGCGGA AGCTGACAGG CATGGAACGT GACCTGGTAG CCATTGAAGC TAAGCTCAGT
    GACCTGCAAA AGGAGGCAGA AAAGTTGGAA TCAGAGCATC CTGACCAGGC GCAAGCTATC
    CTTTCACGGC TTGCGGAAAT CAATGATGTA TGGGAAGAAA TGAAGACCAC CCTGAAAAAC
    CGGGAGGAGT CTCTTGGAGA GGCAAGCAAA CTGCAGCAGT TCCTGAGAGA TCTGGATGAC
    TTCCAGTCTT GGCTATCCAG AACACAGACT GCAATCGCCT CTGAAGACAT GCCGAACACA
    CTGACAGAGG CTGAGAAACT ACTCACTCAA CATGAGAATA TTAAGAACGA AATCAACAAT
    TACGAGGAAG ACTATCAGAA AATGAGGGAC ATGGGTGAGA TGGTGACACA AGGGCAGACT
    GATGCTCAGT ACATGTTCTT ACGCCAGCGC CTACAGGCTT TGGATACTGG ATGGAATGAG
    CTTCACAAAA TGTGGGAGAA CCGGCAAAAT CTTCTTTCCC AGTCTCATGC CTACCAGCTC
    TTTCTCAGAG ATACCAAGCA AGCAGAAGCA TTTCTTAACA ACCAGGAGTA TGTTTTGGCA
    CACACAGAAA TGCCCACTAC CTTGGAAGGA GCAGAAGCTG CTATTAAAAA ACAGGAGGAT
    TTCATGACCA CCATGGATGC GAATGAAGAA AAGATCAATG CAGTTGTAGA GACGGGCAGG
    AGGCTAGTTA GTGATGGGAA CATTAACTCT GACAAAATCC AGGAGAAAGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA CCGTGAAGCA GCCAGCGAGC TTCTGATGAG ACTGAAGGAT
    AACAGGGATC TTCAGAAGTT TCTTCAGGAT TGTCAAGAGC TCTCACTCTG GATCAATGAG
    AAGATGCTTA CAGCCCAGGA TATGTCTTAC GACGAGGCAA GAAACCTGCA CAGCAAATGG
    CTGAAGCATC AGGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT GGAGAAGATT
    GAAAAGGAGG GAATGCAGCT CATTGCAGAG AAACCAGAGA CTGAAGCTGT AGTGAAAGAA
    AAGCTAACAG GTCTTCATCA AATGTGGGAA GAGCTCGAAT CCACTACTCA GACCAAGGCT
    CAGCGTCTCT TTGATGCAAA CAAGGCGGAG CTTTTTACCC AGAGCTGTGC TGATTTGGAT
    AAGTGGCTGA ATGGTTTGGA GAGCCAAATT CAGTCAGATG ACTACGGAAA AGACCTCACC
    AGTGTCAACA TCCTATTGAA GAAGCAGCAG ATGCTAGAGA ATCAAATGGA TGTCCGTAAG
    AAGGAGATAG AAGAGCTACA AAGCCAGGCA CGGGCTCTAA GTCAAGAAGG GAAGAGTACA
    GATGAGGTAG ATGGTAAACG CCTTACCGTT GAAAAGAAAT TTTTGGAGTT GTTGGAACCT
    TTGAATGAAA GAAAAGCAAA CCTGCTGGCC TCCAAAGAAA TCCACCAGTT CAACCGGGAC
    GTGGAAGATG AAATTTTGTG GGTTGGAGAG AGGATGCCTA TAGCAACATC TACTGATCAC
    GGACACAACC TCCAGACTGT GCAGCTACTG ATAAAGAAAA ATCAGACACT TCAGAAAGAA
    ATCCAAGGAC ATCAGCCTCG TATTGATGAT ATTTTTGAGA GGAGTCAAAA CATTATCACA
    GACAGTAGCC CTAATGCTGA AGCAATTCAG CAGAGACTTG CAGACCTGAA GCAGCTGTGG
    AACCTCCTAA TTGAGGAGAC AGAGAAACGC CACAAACGCC TGGAGGAGTC TCACCGGGCA
    CAGCAGTACT ACTTTGATGC TGCCGAGGCT GAGGCCTGGA TGAGTGAGCA GGAGCTTTAC
    ATGATGTCAG AAGAGAAAGC CAAGGATGAA CAGAGTGCGG TATCCATGTT GAAGAAACAT
    CAGATTCTGG AACAGGCTGT GGAAGACTAT GCTGAGACTG TGCACCAGCT TTCAAAGACC
    AGCAGAACTT TGGTGGCAGA TAATCATCCT GAAAGTGAGC GTATCAGCAT GCGCCAGTCC
    AAAGTGGATA AGCTGTATGC AGGCCTGAAG GATCTGGCAG AAGAGAGAAG GGGCAAGCTG
    GATGAGAGAC ACAGGCTGTT CCAGCTGAAC AGAGAGGTGG ATGACCTGGA GCAGTGGATT
    GCTGAAAGGG AGGTGGTGGC AGGATCCCAT GAGCTGGGAC AGGATTATGA ACACGTAACG
    ATGCTACAGG AGCGATTCCG AGAATTTGCC CGAGACACTG GAAACATTGG ACAGGAGCGT
    GTTGATACCG TTAACCATCT GGCTGACGAG CTCATCAACT CTGGGCACTC AGATGCTGCC
    ACAATTGCAG AATGGAAGGA TGGTCTCAAT GAGGCCTGGG CTGATCTCCT GGAGCTCATT
    GACACAAGAA CACAAATTCT TGCAGCCTCT TACGAACTGC ACAAATTTTA CCACGATGCC
    AAGGAAATCT TAGGACGCAT TCAAGACAAA CACAAGAAAC TGCCAGAAGA GCTTGGTAGA
    GACCAAAACA CAGTGGAAAC ACTACAGAGA ATGCACACAA CATTTGAACA TGATATCCAA
    GCGCTCGGCA CACAGGTAAG ACAGCTCCAG GAGGATGCTG CACGCCTTCA GGCTGCCTAT
    GCTGGAGACA AAGCTGATGA CATCCAGAAG AGGGAAAATG AGGTCCTAGA AGCATGGAAG
    GCGCTGTTGG ATGCCTGTGA GGGCCGCAGG GTTAGGCTGG TGGACACAGG AGACAAATTC
    CGTTTCTTCA GCATGGTTCG GGATCTCATG CTTTGGATGG AAGATGTTAT CCGGCAGATC
    GAAGCCCAGG AAAAGCCAAG GGATGTTTCA TCTGTTGAAC TACTAATGAA CAATCACCAG
    GGTATCAAAG CGGAAATTGA TGCCCGAAAT GACAGCTTTA CTACCTGTAT TGAACTCGGC
    AAATCTCTTC TGGCAAGAAA ACACTATGCA TCAGAGGAGA TCAAGGAAAA GCTACTGCAG
    CTGACAGAGA AAAGAAAAGA AATGATTGAC AAGTGGGAAG ACAGATGGGA GTGGCTGAGA
    CTGATTTTGG AGGTGCATCA GTTTTCAAGA GACGCAAGTG TGGCTGAGGC CTGGCTGCTG
    GGACAGGAGC CCTACCTATC CAGTCGTGAA ATAGGTCAGA GTGTTGACGA AGTGGAAAAA
    TTAATTAAGC GGCATGAAGC ATTTGAGAAA TCTGCAGCTA CTTGGGATGA GAGATTTGCA
    GCGCTGGAAA GACTGACTAC GTTGGAACTG CTGGAAGTTC GTCGACAACA AGAGGAAGAA
    GAGAGGAAGA GAAAGCCACC TACTCCAGAG CCAAGCCCCA AAGTTGCAGA AGATGGAGGC
    TCCCAGCAGC AATGGGATGG GACAAAAGGA GAACAGGTTT CCCAAAATGG TTTGCCATCA
    GACCAGGAAT CTCCACGGGT GGCAGAAACT GCGGAAACAA ATGAAATGGT TAATGGAGCT
    GCAGAACAGA GGACAAGTTC GAAAGAGTCT AGTCCCGTTC CTTCTCCAAC AGCAGACCGC
    AAAGCCAAGG CTGCCATTCA GGCTCAGACT GCTGCTACCC TACCAGCCAA AACACAGGAG
    ATTCCTTCAG CTCAGATGGA GGGCTTCTTG CATCGTAAAC ATGAATGGGA GACACACAAC
    AAGAAAGCCT CAAGCAGGTC ATGGCATAAC GTGTACTGTG TCATAAACAA CCAAGAGATG
    GGCTTCTACA AGGATTCAAA AGCTGCTGCT TCTGGCATTC CCTATCACAA TGAGATTCCA
    GTCAGCTTGA AAGAAGCAGT CTGTGAAATA GCAGTCGATT ATAAAAAGAA AAAGCATGTG
    TTCAAGCTAA GATTGACTGA TGGCAATGAA TACCTCTTCC AAGCCAAAGA CGATGAGGAG
    ATGAACACGT GGATTCAGGC CATCACATCA GCTATCTCAT CTGACAAAAT CGAAGTGTCC
    CCGACAACTC AGAGCACTCC AGCCTCCAGC CGTGCCCAGA CCCTGCCTGC CAGCGTCACA
    ATAACCAGTG AGTCCAGTCC TGGCAAGAGA GAGAAAGACA AAGAGAAAGA CAAAGAGAAA
    AGATTCAGCC TTTTTGGTAA AAAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138837.1
    CDS380..7429
    Translation

    Target ORF information:

    RefSeq Version XM_015283351.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015283351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTAC AAAAATCAAC CAGTATGCCT GGGCCGTTGT CCCCAGGATA CGCAGCTCAG 
    GTGCCCTATA ACTATAATCA GTTAGAAGGA AGATTCAAGC AGTTACAAGA TGAACGTGAG
    GCAGTTCAGA AGAAGACTTT CACCAAGTGG GTTAATTCCC ACTTGGCACG TGTGTCATGC
    AGAATTACAG ATTTGTACAC CGACCTTCGC GATGGAAGGA TGCTCATCAA ACTCCTGGAA
    GTTCTTTCAG GAGAGAGACT TCCCAAACCA ACTAAGGGAA GGATGCGCAT CCATTGTTTG
    GAGAATGTTG ATAAAGCCCT GCAGTTCCTG AAGGAGCAAC GAGTGCACCT GGAAAACATG
    GGATCTCATG ATATTGTGGA TGGGAATCAC AGGCTGACTC TTGGCCTTAT TTGGACTATT
    ATCCTGAGAT TCCAGATCCA AGATATCAGT GTTGAAACTG AGGACAACAA GGAGAAGAAG
    TCTGCTAAGG ATGCACTGCT TTTGTGGTGC CAGATGAAGA CAGCTGGTTA CCCCAACGTC
    AACATTCACA ACTTTACAAC AAGCTGGAGG GATGGAATGG CTTTCAATGC ATTGATACAC
    AAGCACAGGC CTGATCTGAT TGATTTTGAC AAGCTGAAGA AATCAAATGC ACACTACAAT
    CTGCAGAATG CTTTTAACCT TGCTGAGCAA CACCTTGGTC TTACTAAGCT CCTGGACCCT
    GAGGATATCA GTGTTGATCA TCCCGATGAA AAGTCAATCA TCACCTATGT GGTGACATAT
    TACCACTACT TCTCTAAAAT GAAAGCTTTA GCTGTTGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGACAATG CCATTGAAAC AGAGAAAATG ATTGAAAAAT ATGAATCGCT GGCTTCTGAT
    CTTCTGGAGT GGATTGAGCA AACCATTATC ATCCTCAACA ACCGCAAATT TGCCAACTCG
    CTAGTTGGTG TGCAACAGCA GCTGCAGGCA TTCAACACCT ACCGCACAGT AGAGAAACCA
    CCCAAATTTA CAGAAAAAGG AAACCTGGAA GTGCTGCTTT TTACCATCCA AAGCAAAATG
    AGAGCCAACA ATCAGAAAGT ATACATGCCA AGGGAGGGCA AACTCATCTC TGACATAAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCTGAA CATGAAAGAG AACTGGCACT GCGAAATGAG
    CTCATCAGAC AAGAGAAACT GGAACAACTT GCACGCCGAT TTGATCGCAA GGCAGCTATG
    AGAGAAACTT GGCTGAGTGA AAATCAACGT CTGGTTTCTC AGGATAACTT TGGCTTTGAC
    CTTCCAGCAG TAGAGGCTGC AACGAAAAAG CACGAGGCCA TTGAAACAGA CATTGCAGCA
    TATGAAGAGC GAGTCCAGGC TGTGGTTGCA GTTGCAAAGG AGCTTGAGAC TGAAAATTAT
    CATGATATCA AACGTATCAC TGCAAGAAAG GACAATGTTA TACGCCTGTG GGAGTACCTC
    CTGGAGCTAC TCAGGGCACG AAGACAACGG CTGGAAATGA ATCTTGGCCT ACAAAAGATT
    TTCCAGGAAA TGCTATACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TCTGCTGTCA
    CAAGACTATG GCAAACATTT GTTAGGAGTC GAAGACCTGT TGCAGAAACA TGCTCTAGTG
    GAAGCTGACA TTGCTATTCA GGCTGAGAGA GTGAGAGGGG TCAATGCATC TGCTCAGAAG
    TTTGCCACTG ATGGAGAAGG TTACAAGCCA TGTGACCCAC AGGTTATTAG GGATCGCGTC
    GCTCACATGG AGTTCTGTTA CCAAGAGCTG TGCCAGCTTT CAGCTGAGCG CCGGGCCCGC
    TTGGAGGAGT CCCGGCGCCT CTGGAAATTC TTCTGGGAAA TGGCTGAAGA AGAGGGTTGG
    ATCAGGGAGA AGGAGCAGAT CCTTTCATCT GATGACTACG GGAAGGACCT GACCAGCGTC
    GTCCGTCTCA TGAGTAAACA TAAGGCATTT GAAGATGAAA TGAGTGGTCG CAGTGGCCAC
    TTTCAGCAGG CGATAAAAGA AGGTGAAGAC ATGATCGCGG AAGAGAATTT TGGGTCTGAG
    AAAATCAGAG AAAGAATTAA GGATATTCGG GAGCAGTGGG CTAACTTGGA ACAGTTATCT
    GCCATTAGGA AGAAACGTCT GGAGGAAGCC TCTCTCCTTC ACCAGTTCCA GGCTGATGCT
    GATGACATTG ATGCATGGAT GTTGGACATC CTCAAAATTG TCTCCAGCAA TGATGTTGGC
    CATGACGAAT ATTCCACTCA GTCCTTGGTC AAGAAACACA AGGATGTTGC TGAAGAGATT
    GCAAGCTATC GGCCAACCAT TGACTCGCTT CATGAACAGG CAAAGGCTCT ACCACAGGAG
    CATGCCGGGT CTCCAGATGT GCAAGGCAGG TTGTCTGGAA TAGAAGAAAG ATACAAAGAG
    GTTGCTGAGC TAACTCGGCT GCGTAAACAG GCTTTGCAGG ATACCCTTGC TCTTTATAAG
    ATGTTCAGTG AGGCAGATGC TTGTGAGCTT TGGATTGATG AGAAGGAGAA GTGGCTGAAT
    AATATGCAGA TTCCAGAGAA GCTGGAGGAC CTGGAAGTGA TCCAGCACAG ATTTGAAAGC
    TTAGAACCAG AAATGAACAA TCAGGCTTCC CGTGTTGCTG TAGTGAACCA AATTGCAAGA
    CAGCTAATGC ATAGTGGCCA TCCAAGTGAG AAGGAGATAA AAGCACAGCA AGATAAACTC
    AACACAAGAT GGAGCCAGTT CAGAGAACTG GTAGACAGGA AGAAAGATGC CCTGATCTCT
    GCCTTAAGTA TCCAGAATTA CCACCTTGAA TGTAATGAAA CCAAGTCCTG GATCAGGGAG
    AAAACCAAAG TGATTGAATC CACACAAGAT TTGGGTAACG ATCTTGCTGG AGTGATGGCT
    CTGCAGCGGA AGCTGACAGG CATGGAACGT GACCTGGTAG CCATTGAAGC TAAGCTCAGT
    GACCTGCAAA AGGAGGCAGA AAAGTTGGAA TCAGAGCATC CTGACCAGGC GCAAGCTATC
    CTTTCACGGC TTGCGGAAAT CAATGATGTA TGGGAAGAAA TGAAGACCAC CCTGAAAAAC
    CGGGAGGAGT CTCTTGGAGA GGCAAGCAAA CTGCAGCAGT TCCTGAGAGA TCTGGATGAC
    TTCCAGTCTT GGCTATCCAG AACACAGACT GCAATCGCCT CTGAAGACAT GCCGAACACA
    CTGACAGAGG CTGAGAAACT ACTCACTCAA CATGAGAATA TTAAGAACGA AATCAACAAT
    TACGAGGAAG ACTATCAGAA AATGAGGGAC ATGGGTGAGA TGGTGACACA AGGGCAGACT
    GATGCTCAGT ACATGTTCTT ACGCCAGCGC CTACAGGCTT TGGATACTGG ATGGAATGAG
    CTTCACAAAA TGTGGGAGAA CCGGCAAAAT CTTCTTTCCC AGTCTCATGC CTACCAGCTC
    TTTCTCAGAG ATACCAAGCA AGCAGAAGCA TTTCTTAACA ACCAGGAGTA TGTTTTGGCA
    CACACAGAAA TGCCCACTAC CTTGGAAGGA GCAGAAGCTG CTATTAAAAA ACAGGAGGAT
    TTCATGACCA CCATGGATGC GAATGAAGAA AAGATCAATG CAGTTGTAGA GACGGGCAGG
    AGGCTAGTTA GTGATGGGAA CATTAACTCT GACAAAATCC AGGAGAAAGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA CCGTGAAGCA GCCAGCGAGC TTCTGATGAG ACTGAAGGAT
    AACAGGGATC TTCAGAAGTT TCTTCAGGAT TGTCAAGAGC TCTCACTCTG GATCAATGAG
    AAGATGCTTA CAGCCCAGGA TATGTCTTAC GACGAGGCAA GAAACCTGCA CAGCAAATGG
    CTGAAGCATC AGGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT GGAGAAGATT
    GAAAAGGAGG GAATGCAGCT CATTGCAGAG AAACCAGAGA CTGAAGCTGT AGTGAAAGAA
    AAGCTAACAG GTCTTCATCA AATGTGGGAA GAGCTCGAAT CCACTACTCA GACCAAGGCT
    CAGCGTCTCT TTGATGCAAA CAAGGCGGAG CTTTTTACCC AGAGCTGTGC TGATTTGGAT
    AAGTGGCTGA ATGGTTTGGA GAGCCAAATT CAGTCAGATG ACTACGGAAA AGACCTCACC
    AGTGTCAACA TCCTATTGAA GAAGCAGCAG ATGCTAGAGA ATCAAATGGA TGTCCGTAAG
    AAGGAGATAG AAGAGCTACA AAGCCAGGCA CGGGCTCTAA GTCAAGAAGG GAAGAGTACA
    GATGAGGTAG ATGGTAAACG CCTTACCGTT GAAAAGAAAT TTTTGGAGTT GTTGGAACCT
    TTGAATGAAA GAAAAGCAAA CCTGCTGGCC TCCAAAGAAA TCCACCAGTT CAACCGGGAC
    GTGGAAGATG AAATTTTGTG GGTTGGAGAG AGGATGCCTA TAGCAACATC TACTGATCAC
    GGACACAACC TCCAGACTGT GCAGCTACTG ATAAAGAAAA ATCAGACACT TCAGAAAGAA
    ATCCAAGGAC ATCAGCCTCG TATTGATGAT ATTTTTGAGA GGAGTCAAAA CATTATCACA
    GACAGTAGCC CTAATGCTGA AGCAATTCAG CAGAGACTTG CAGACCTGAA GCAGCTGTGG
    AACCTCCTAA TTGAGGAGAC AGAGAAACGC CACAAACGCC TGGAGGAGTC TCACCGGGCA
    CAGCAGTACT ACTTTGATGC TGCCGAGGCT GAGGCCTGGA TGAGTGAGCA GGAGCTTTAC
    ATGATGTCAG AAGAGAAAGC CAAGGATGAA CAGAGTGCGG TATCCATGTT GAAGAAACAT
    CAGATTCTGG AACAGGCTGT GGAAGACTAT GCTGAGACTG TGCACCAGCT TTCAAAGACC
    AGCAGAACTT TGGTGGCAGA TAATCATCCT GAAAGTGAGC GTATCAGCAT GCGCCAGTCC
    AAAGTGGATA AGCTGTATGC AGGCCTGAAG GATCTGGCAG AAGAGAGAAG GGGCAAGCTG
    GATGAGAGAC ACAGGCTGTT CCAGCTGAAC AGAGAGGTGG ATGACCTGGA GCAGTGGATT
    GCTGAAAGGG AGGTGGTGGC AGGATCCCAT GAGCTGGGAC AGGATTATGA ACACGTAACG
    ATGCTACAGG AGCGATTCCG AGAATTTGCC CGAGACACTG GAAACATTGG ACAGGAGCGT
    GTTGATACCG TTAACCATCT GGCTGACGAG CTCATCAACT CTGGGCACTC AGATGCTGCC
    ACAATTGCAG AATGGAAGGA TGGTCTCAAT GAGGCCTGGG CTGATCTCCT GGAGCTCATT
    GACACAAGAA CACAAATTCT TGCAGCCTCT TACGAACTGC ACAAATTTTA CCACGATGCC
    AAGGAAATCT TAGGACGCAT TCAAGACAAA CACAAGAAAC TGCCAGAAGA GCTTGGTAGA
    GACCAAAACA CAGTGGAAAC ACTACAGAGA ATGCACACAA CATTTGAACA TGATATCCAA
    GCGCTCGGCA CACAGGTAAG ACAGCTCCAG GAGGATGCTG CACGCCTTCA GGCTGCCTAT
    GCTGGAGACA AAGCTGATGA CATCCAGAAG AGGGAAAATG AGGTCCTAGA AGCATGGAAG
    GCGCTGTTGG ATGCCTGTGA GGGCCGCAGG GTTAGGCTGG TGGACACAGG AGACAAATTC
    CGTTTCTTCA GCATGGTTCG GGATCTCATG CTTTGGATGG AAGATGTTAT CCGGCAGATC
    GAAGCCCAGG AAAAGCCAAG GGATGTTTCA TCTGTTGAAC TACTAATGAA CAATCACCAG
    GGTATCAAAG CGGAAATTGA TGCCCGAAAT GACAGCTTTA CTACCTGTAT TGAACTCGGC
    AAATCTCTTC TGGCAAGAAA ACACTATGCA TCAGAGGAGA TCAAGGAAAA GCTACTGCAG
    CTGACAGAGA AAAGAAAAGA AATGATTGAC AAGTGGGAAG ACAGATGGGA GTGGCTGAGA
    CTGATTTTGG AGGTGCATCA GTTTTCAAGA GACGCAAGTG TGGCTGAGGC CTGGCTGCTG
    GGACAGGAGC CCTACCTATC CAGTCGTGAA ATAGGTCAGA GTGTTGACGA AGTGGAAAAA
    TTAATTAAGC GGCATGAAGC ATTTGAGAAA TCTGCAGCTA CTTGGGATGA GAGATTTGCA
    GCGCTGGAAA GACTGACTAC GTTGGAACTG CTGGAAGTTC GTCGACAACA AGAGGAAGAA
    GAGAGGAAGA GAAAGCCACC TACTCCAGAG CCAAGCCCCA AAGTTGCAGA AGATGGAGGC
    TCCCAGCAGC AATGGGATGG GACAAAAGGA GAACAGGTTT CCCAAAATGG TTTGCCATCA
    GACCAGGAAT CTCCACGGGT GGCAGAAACT GCGGAAACAA ATGAAATGGT TAATGGAGCT
    GCAGAACAGA GGACAAGTTC GAAAGAGTCT AGTCCCGTTC CTTCTCCAAC AGCAGACCGC
    AAAGCCAAGG CTGCCATTCA GGCTCAGACT GCTGCTACCC TACCAGCCAA AACACAGGAG
    ATTCCTTCAG CTCAGATGGA GGGCTTCTTG CATCGTAAAC ATGAATGGGA GACACACAAC
    AAGAAAGCCT CAAGCAGGTC ATGGCATAAC GTGTACTGTG TCATAAACAA CCAAGAGATG
    GGCTTCTACA AGGATTCAAA AGCTGCTGCT TCTGGCATTC CCTATCACAA TGAGATTCCA
    GTCAGCTTGA AAGAAGCAGT CTGTGAAATA GCAGTCGATT ATAAAAAGAA AAAGCATGTG
    TTCAAGCTAA GATTGACTGA TGGCAATGAA TACCTCTTCC AAGCCAAAGA CGATGAGGAG
    ATGAACACGT GGATTCAGGC CATCACATCA GCTATCTCAT CTGACAAAAT CGAAGTGTCC
    CCGACAACTC AGAGCACTCC AGCCTCCAGC CGTGCCCAGA CCCTGCCTGC CAGCGTCACA
    ATAACCAGTG AGTCCAGTCC TGGCAAGAGA GAGAAAGACA AAGAGAAAGA CAAAGAGAAA
    AGATTCAGCC TTTTTGGTAA AAAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33037
    Clone ID Related Accession (Same CDS sequence) XM_015283351.1 , XM_015283351.2
    Accession Version XM_015283351.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTAC AAAAATCAAC CAGTATGCCT GGGCCGTTGT CCCCAGGATA CGCAGCTCAG 
    GTGCCCTATA ACTATAATCA GTTAGAAGGA AGATTCAAGC AGTTACAAGA TGAACGTGAG
    GCAGTTCAGA AGAAGACTTT CACCAAGTGG GTTAATTCCC ACTTGGCACG TGTGTCATGC
    AGAATTACAG ATTTGTACAC CGACCTTCGC GATGGAAGGA TGCTCATCAA ACTCCTGGAA
    GTTCTTTCAG GAGAGAGACT TCCCAAACCA ACTAAGGGAA GGATGCGCAT CCATTGTTTG
    GAGAATGTTG ATAAAGCCCT GCAGTTCCTG AAGGAGCAAC GAGTGCACCT GGAAAACATG
    GGATCTCATG ATATTGTGGA TGGGAATCAC AGGCTGACTC TTGGCCTTAT TTGGACTATT
    ATCCTGAGAT TCCAGATCCA AGATATCAGT GTTGAAACTG AGGACAACAA GGAGAAGAAG
    TCTGCTAAGG ATGCACTGCT TTTGTGGTGC CAGATGAAGA CAGCTGGTTA CCCCAACGTC
    AACATTCACA ACTTTACAAC AAGCTGGAGG GATGGAATGG CTTTCAATGC ATTGATACAC
    AAGCACAGGC CTGATCTGAT TGATTTTGAC AAGCTGAAGA AATCAAATGC ACACTACAAT
    CTGCAGAATG CTTTTAACCT TGCTGAGCAA CACCTTGGTC TTACTAAGCT CCTGGACCCT
    GAGGATATCA GTGTTGATCA TCCCGATGAA AAGTCAATCA TCACCTATGT GGTGACATAT
    TACCACTACT TCTCTAAAAT GAAAGCTTTA GCTGTTGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGACAATG CCATTGAAAC AGAGAAAATG ATTGAAAAAT ATGAATCGCT GGCTTCTGAT
    CTTCTGGAGT GGATTGAGCA AACCATTATC ATCCTCAACA ACCGCAAATT TGCCAACTCG
    CTAGTTGGTG TGCAACAGCA GCTGCAGGCA TTCAACACCT ACCGCACAGT AGAGAAACCA
    CCCAAATTTA CAGAAAAAGG AAACCTGGAA GTGCTGCTTT TTACCATCCA AAGCAAAATG
    AGAGCCAACA ATCAGAAAGT ATACATGCCA AGGGAGGGCA AACTCATCTC TGACATAAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCTGAA CATGAAAGAG AACTGGCACT GCGAAATGAG
    CTCATCAGAC AAGAGAAACT GGAACAACTT GCACGCCGAT TTGATCGCAA GGCAGCTATG
    AGAGAAACTT GGCTGAGTGA AAATCAACGT CTGGTTTCTC AGGATAACTT TGGCTTTGAC
    CTTCCAGCAG TAGAGGCTGC AACGAAAAAG CACGAGGCCA TTGAAACAGA CATTGCAGCA
    TATGAAGAGC GAGTCCAGGC TGTGGTTGCA GTTGCAAAGG AGCTTGAGAC TGAAAATTAT
    CATGATATCA AACGTATCAC TGCAAGAAAG GACAATGTTA TACGCCTGTG GGAGTACCTC
    CTGGAGCTAC TCAGGGCACG AAGACAACGG CTGGAAATGA ATCTTGGCCT ACAAAAGATT
    TTCCAGGAAA TGCTATACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TCTGCTGTCA
    CAAGACTATG GCAAACATTT GTTAGGAGTC GAAGACCTGT TGCAGAAACA TGCTCTAGTG
    GAAGCTGACA TTGCTATTCA GGCTGAGAGA GTGAGAGGGG TCAATGCATC TGCTCAGAAG
    TTTGCCACTG ATGGAGAAGG TTACAAGCCA TGTGACCCAC AGGTTATTAG GGATCGCGTC
    GCTCACATGG AGTTCTGTTA CCAAGAGCTG TGCCAGCTTT CAGCTGAGCG CCGGGCCCGC
    TTGGAGGAGT CCCGGCGCCT CTGGAAATTC TTCTGGGAAA TGGCTGAAGA AGAGGGTTGG
    ATCAGGGAGA AGGAGCAGAT CCTTTCATCT GATGACTACG GGAAGGACCT GACCAGCGTC
    GTCCGTCTCA TGAGTAAACA TAAGGCATTT GAAGATGAAA TGAGTGGTCG CAGTGGCCAC
    TTTCAGCAGG CGATAAAAGA AGGTGAAGAC ATGATCGCGG AAGAGAATTT TGGGTCTGAG
    AAAATCAGAG AAAGAATTAA GGATATTCGG GAGCAGTGGG CTAACTTGGA ACAGTTATCT
    GCCATTAGGA AGAAACGTCT GGAGGAAGCC TCTCTCCTTC ACCAGTTCCA GGCTGATGCT
    GATGACATTG ATGCATGGAT GTTGGACATC CTCAAAATTG TCTCCAGCAA TGATGTTGGC
    CATGACGAAT ATTCCACTCA GTCCTTGGTC AAGAAACACA AGGATGTTGC TGAAGAGATT
    GCAAGCTATC GGCCAACCAT TGACTCGCTT CATGAACAGG CAAAGGCTCT ACCACAGGAG
    CATGCCGGGT CTCCAGATGT GCAAGGCAGG TTGTCTGGAA TAGAAGAAAG ATACAAAGAG
    GTTGCTGAGC TAACTCGGCT GCGTAAACAG GCTTTGCAGG ATACCCTTGC TCTTTATAAG
    ATGTTCAGTG AGGCAGATGC TTGTGAGCTT TGGATTGATG AGAAGGAGAA GTGGCTGAAT
    AATATGCAGA TTCCAGAGAA GCTGGAGGAC CTGGAAGTGA TCCAGCACAG ATTTGAAAGC
    TTAGAACCAG AAATGAACAA TCAGGCTTCC CGTGTTGCTG TAGTGAACCA AATTGCAAGA
    CAGCTAATGC ATAGTGGCCA TCCAAGTGAG AAGGAGATAA AAGCACAGCA AGATAAACTC
    AACACAAGAT GGAGCCAGTT CAGAGAACTG GTAGACAGGA AGAAAGATGC CCTGATCTCT
    GCCTTAAGTA TCCAGAATTA CCACCTTGAA TGTAATGAAA CCAAGTCCTG GATCAGGGAG
    AAAACCAAAG TGATTGAATC CACACAAGAT TTGGGTAACG ATCTTGCTGG AGTGATGGCT
    CTGCAGCGGA AGCTGACAGG CATGGAACGT GACCTGGTAG CCATTGAAGC TAAGCTCAGT
    GACCTGCAAA AGGAGGCAGA AAAGTTGGAA TCAGAGCATC CTGACCAGGC GCAAGCTATC
    CTTTCACGGC TTGCGGAAAT CAATGATGTA TGGGAAGAAA TGAAGACCAC CCTGAAAAAC
    CGGGAGGAGT CTCTTGGAGA GGCAAGCAAA CTGCAGCAGT TCCTGAGAGA TCTGGATGAC
    TTCCAGTCTT GGCTATCCAG AACACAGACT GCAATCGCCT CTGAAGACAT GCCGAACACA
    CTGACAGAGG CTGAGAAACT ACTCACTCAA CATGAGAATA TTAAGAACGA AATCAACAAT
    TACGAGGAAG ACTATCAGAA AATGAGGGAC ATGGGTGAGA TGGTGACACA AGGGCAGACT
    GATGCTCAGT ACATGTTCTT ACGCCAGCGC CTACAGGCTT TGGATACTGG ATGGAATGAG
    CTTCACAAAA TGTGGGAGAA CCGGCAAAAT CTTCTTTCCC AGTCTCATGC CTACCAGCTC
    TTTCTCAGAG ATACCAAGCA AGCAGAAGCA TTTCTTAACA ACCAGGAGTA TGTTTTGGCA
    CACACAGAAA TGCCCACTAC CTTGGAAGGA GCAGAAGCTG CTATTAAAAA ACAGGAGGAT
    TTCATGACCA CCATGGATGC GAATGAAGAA AAGATCAATG CAGTTGTAGA GACGGGCAGG
    AGGCTAGTTA GTGATGGGAA CATTAACTCT GACAAAATCC AGGAGAAAGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA CCGTGAAGCA GCCAGCGAGC TTCTGATGAG ACTGAAGGAT
    AACAGGGATC TTCAGAAGTT TCTTCAGGAT TGTCAAGAGC TCTCACTCTG GATCAATGAG
    AAGATGCTTA CAGCCCAGGA TATGTCTTAC GACGAGGCAA GAAACCTGCA CAGCAAATGG
    CTGAAGCATC AGGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT GGAGAAGATT
    GAAAAGGAGG GAATGCAGCT CATTGCAGAG AAACCAGAGA CTGAAGCTGT AGTGAAAGAA
    AAGCTAACAG GTCTTCATCA AATGTGGGAA GAGCTCGAAT CCACTACTCA GACCAAGGCT
    CAGCGTCTCT TTGATGCAAA CAAGGCGGAG CTTTTTACCC AGAGCTGTGC TGATTTGGAT
    AAGTGGCTGA ATGGTTTGGA GAGCCAAATT CAGTCAGATG ACTACGGAAA AGACCTCACC
    AGTGTCAACA TCCTATTGAA GAAGCAGCAG ATGCTAGAGA ATCAAATGGA TGTCCGTAAG
    AAGGAGATAG AAGAGCTACA AAGCCAGGCA CGGGCTCTAA GTCAAGAAGG GAAGAGTACA
    GATGAGGTAG ATGGTAAACG CCTTACCGTT GAAAAGAAAT TTTTGGAGTT GTTGGAACCT
    TTGAATGAAA GAAAAGCAAA CCTGCTGGCC TCCAAAGAAA TCCACCAGTT CAACCGGGAC
    GTGGAAGATG AAATTTTGTG GGTTGGAGAG AGGATGCCTA TAGCAACATC TACTGATCAC
    GGACACAACC TCCAGACTGT GCAGCTACTG ATAAAGAAAA ATCAGACACT TCAGAAAGAA
    ATCCAAGGAC ATCAGCCTCG TATTGATGAT ATTTTTGAGA GGAGTCAAAA CATTATCACA
    GACAGTAGCC CTAATGCTGA AGCAATTCAG CAGAGACTTG CAGACCTGAA GCAGCTGTGG
    AACCTCCTAA TTGAGGAGAC AGAGAAACGC CACAAACGCC TGGAGGAGTC TCACCGGGCA
    CAGCAGTACT ACTTTGATGC TGCCGAGGCT GAGGCCTGGA TGAGTGAGCA GGAGCTTTAC
    ATGATGTCAG AAGAGAAAGC CAAGGATGAA CAGAGTGCGG TATCCATGTT GAAGAAACAT
    CAGATTCTGG AACAGGCTGT GGAAGACTAT GCTGAGACTG TGCACCAGCT TTCAAAGACC
    AGCAGAACTT TGGTGGCAGA TAATCATCCT GAAAGTGAGC GTATCAGCAT GCGCCAGTCC
    AAAGTGGATA AGCTGTATGC AGGCCTGAAG GATCTGGCAG AAGAGAGAAG GGGCAAGCTG
    GATGAGAGAC ACAGGCTGTT CCAGCTGAAC AGAGAGGTGG ATGACCTGGA GCAGTGGATT
    GCTGAAAGGG AGGTGGTGGC AGGATCCCAT GAGCTGGGAC AGGATTATGA ACACGTAACG
    ATGCTACAGG AGCGATTCCG AGAATTTGCC CGAGACACTG GAAACATTGG ACAGGAGCGT
    GTTGATACCG TTAACCATCT GGCTGACGAG CTCATCAACT CTGGGCACTC AGATGCTGCC
    ACAATTGCAG AATGGAAGGA TGGTCTCAAT GAGGCCTGGG CTGATCTCCT GGAGCTCATT
    GACACAAGAA CACAAATTCT TGCAGCCTCT TACGAACTGC ACAAATTTTA CCACGATGCC
    AAGGAAATCT TAGGACGCAT TCAAGACAAA CACAAGAAAC TGCCAGAAGA GCTTGGTAGA
    GACCAAAACA CAGTGGAAAC ACTACAGAGA ATGCACACAA CATTTGAACA TGATATCCAA
    GCGCTCGGCA CACAGGTAAG ACAGCTCCAG GAGGATGCTG CACGCCTTCA GGCTGCCTAT
    GCTGGAGACA AAGCTGATGA CATCCAGAAG AGGGAAAATG AGGTCCTAGA AGCATGGAAG
    GCGCTGTTGG ATGCCTGTGA GGGCCGCAGG GTTAGGCTGG TGGACACAGG AGACAAATTC
    CGTTTCTTCA GCATGGTTCG GGATCTCATG CTTTGGATGG AAGATGTTAT CCGGCAGATC
    GAAGCCCAGG AAAAGCCAAG GGATGTTTCA TCTGTTGAAC TACTAATGAA CAATCACCAG
    GGTATCAAAG CGGAAATTGA TGCCCGAAAT GACAGCTTTA CTACCTGTAT TGAACTCGGC
    AAATCTCTTC TGGCAAGAAA ACACTATGCA TCAGAGGAGA TCAAGGAAAA GCTACTGCAG
    CTGACAGAGA AAAGAAAAGA AATGATTGAC AAGTGGGAAG ACAGATGGGA GTGGCTGAGA
    CTGATTTTGG AGGTGCATCA GTTTTCAAGA GACGCAAGTG TGGCTGAGGC CTGGCTGCTG
    GGACAGGAGC CCTACCTATC CAGTCGTGAA ATAGGTCAGA GTGTTGACGA AGTGGAAAAA
    TTAATTAAGC GGCATGAAGC ATTTGAGAAA TCTGCAGCTA CTTGGGATGA GAGATTTGCA
    GCGCTGGAAA GACTGACTAC GTTGGAACTG CTGGAAGTTC GTCGACAACA AGAGGAAGAA
    GAGAGGAAGA GAAAGCCACC TACTCCAGAG CCAAGCCCCA AAGTTGCAGA AGATGGAGGC
    TCCCAGCAGC AATGGGATGG GACAAAAGGA GAACAGGTTT CCCAAAATGG TTTGCCATCA
    GACCAGGAAT CTCCACGGGT GGCAGAAACT GCGGAAACAA ATGAAATGGT TAATGGAGCT
    GCAGAACAGA GGACAAGTTC GAAAGAGTCT AGTCCCGTTC CTTCTCCAAC AGCAGACCGC
    AAAGCCAAGG CTGCCATTCA GGCTCAGACT GCTGCTACCC TACCAGCCAA AACACAGGAG
    ATTCCTTCAG CTCAGATGGA GGGCTTCTTG CATCGTAAAC ATGAATGGGA GACACACAAC
    AAGAAAGCCT CAAGCAGGTC ATGGCATAAC GTGTACTGTG TCATAAACAA CCAAGAGATG
    GGCTTCTACA AGGATTCAAA AGCTGCTGCT TCTGGCATTC CCTATCACAA TGAGATTCCA
    GTCAGCTTGA AAGAAGCAGT CTGTGAAATA GCAGTCGATT ATAAAAAGAA AAAGCATGTG
    TTCAAGCTAA GATTGACTGA TGGCAATGAA TACCTCTTCC AAGCCAAAGA CGATGAGGAG
    ATGAACACGT GGATTCAGGC CATCACATCA GCTATCTCAT CTGACAAAAT CGAAGTGTCC
    CCGACAACTC AGAGCACTCC AGCCTCCAGC CGTGCCCAGA CCCTGCCTGC CAGCGTCACA
    ATAACCAGTG AGTCCAGTCC TGGCAAGAGA GAGAAAGACA AAGAGAAAGA CAAAGAGAAA
    AGATTCAGCC TTTTTGGTAA AAAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138837.1
    CDS385..7434
    Translation

    Target ORF information:

    RefSeq Version XM_015283351.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015283351.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTAC AAAAATCAAC CAGTATGCCT GGGCCGTTGT CCCCAGGATA CGCAGCTCAG 
    GTGCCCTATA ACTATAATCA GTTAGAAGGA AGATTCAAGC AGTTACAAGA TGAACGTGAG
    GCAGTTCAGA AGAAGACTTT CACCAAGTGG GTTAATTCCC ACTTGGCACG TGTGTCATGC
    AGAATTACAG ATTTGTACAC CGACCTTCGC GATGGAAGGA TGCTCATCAA ACTCCTGGAA
    GTTCTTTCAG GAGAGAGACT TCCCAAACCA ACTAAGGGAA GGATGCGCAT CCATTGTTTG
    GAGAATGTTG ATAAAGCCCT GCAGTTCCTG AAGGAGCAAC GAGTGCACCT GGAAAACATG
    GGATCTCATG ATATTGTGGA TGGGAATCAC AGGCTGACTC TTGGCCTTAT TTGGACTATT
    ATCCTGAGAT TCCAGATCCA AGATATCAGT GTTGAAACTG AGGACAACAA GGAGAAGAAG
    TCTGCTAAGG ATGCACTGCT TTTGTGGTGC CAGATGAAGA CAGCTGGTTA CCCCAACGTC
    AACATTCACA ACTTTACAAC AAGCTGGAGG GATGGAATGG CTTTCAATGC ATTGATACAC
    AAGCACAGGC CTGATCTGAT TGATTTTGAC AAGCTGAAGA AATCAAATGC ACACTACAAT
    CTGCAGAATG CTTTTAACCT TGCTGAGCAA CACCTTGGTC TTACTAAGCT CCTGGACCCT
    GAGGATATCA GTGTTGATCA TCCCGATGAA AAGTCAATCA TCACCTATGT GGTGACATAT
    TACCACTACT TCTCTAAAAT GAAAGCTTTA GCTGTTGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGACAATG CCATTGAAAC AGAGAAAATG ATTGAAAAAT ATGAATCGCT GGCTTCTGAT
    CTTCTGGAGT GGATTGAGCA AACCATTATC ATCCTCAACA ACCGCAAATT TGCCAACTCG
    CTAGTTGGTG TGCAACAGCA GCTGCAGGCA TTCAACACCT ACCGCACAGT AGAGAAACCA
    CCCAAATTTA CAGAAAAAGG AAACCTGGAA GTGCTGCTTT TTACCATCCA AAGCAAAATG
    AGAGCCAACA ATCAGAAAGT ATACATGCCA AGGGAGGGCA AACTCATCTC TGACATAAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCTGAA CATGAAAGAG AACTGGCACT GCGAAATGAG
    CTCATCAGAC AAGAGAAACT GGAACAACTT GCACGCCGAT TTGATCGCAA GGCAGCTATG
    AGAGAAACTT GGCTGAGTGA AAATCAACGT CTGGTTTCTC AGGATAACTT TGGCTTTGAC
    CTTCCAGCAG TAGAGGCTGC AACGAAAAAG CACGAGGCCA TTGAAACAGA CATTGCAGCA
    TATGAAGAGC GAGTCCAGGC TGTGGTTGCA GTTGCAAAGG AGCTTGAGAC TGAAAATTAT
    CATGATATCA AACGTATCAC TGCAAGAAAG GACAATGTTA TACGCCTGTG GGAGTACCTC
    CTGGAGCTAC TCAGGGCACG AAGACAACGG CTGGAAATGA ATCTTGGCCT ACAAAAGATT
    TTCCAGGAAA TGCTATACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TCTGCTGTCA
    CAAGACTATG GCAAACATTT GTTAGGAGTC GAAGACCTGT TGCAGAAACA TGCTCTAGTG
    GAAGCTGACA TTGCTATTCA GGCTGAGAGA GTGAGAGGGG TCAATGCATC TGCTCAGAAG
    TTTGCCACTG ATGGAGAAGG TTACAAGCCA TGTGACCCAC AGGTTATTAG GGATCGCGTC
    GCTCACATGG AGTTCTGTTA CCAAGAGCTG TGCCAGCTTT CAGCTGAGCG CCGGGCCCGC
    TTGGAGGAGT CCCGGCGCCT CTGGAAATTC TTCTGGGAAA TGGCTGAAGA AGAGGGTTGG
    ATCAGGGAGA AGGAGCAGAT CCTTTCATCT GATGACTACG GGAAGGACCT GACCAGCGTC
    GTCCGTCTCA TGAGTAAACA TAAGGCATTT GAAGATGAAA TGAGTGGTCG CAGTGGCCAC
    TTTCAGCAGG CGATAAAAGA AGGTGAAGAC ATGATCGCGG AAGAGAATTT TGGGTCTGAG
    AAAATCAGAG AAAGAATTAA GGATATTCGG GAGCAGTGGG CTAACTTGGA ACAGTTATCT
    GCCATTAGGA AGAAACGTCT GGAGGAAGCC TCTCTCCTTC ACCAGTTCCA GGCTGATGCT
    GATGACATTG ATGCATGGAT GTTGGACATC CTCAAAATTG TCTCCAGCAA TGATGTTGGC
    CATGACGAAT ATTCCACTCA GTCCTTGGTC AAGAAACACA AGGATGTTGC TGAAGAGATT
    GCAAGCTATC GGCCAACCAT TGACTCGCTT CATGAACAGG CAAAGGCTCT ACCACAGGAG
    CATGCCGGGT CTCCAGATGT GCAAGGCAGG TTGTCTGGAA TAGAAGAAAG ATACAAAGAG
    GTTGCTGAGC TAACTCGGCT GCGTAAACAG GCTTTGCAGG ATACCCTTGC TCTTTATAAG
    ATGTTCAGTG AGGCAGATGC TTGTGAGCTT TGGATTGATG AGAAGGAGAA GTGGCTGAAT
    AATATGCAGA TTCCAGAGAA GCTGGAGGAC CTGGAAGTGA TCCAGCACAG ATTTGAAAGC
    TTAGAACCAG AAATGAACAA TCAGGCTTCC CGTGTTGCTG TAGTGAACCA AATTGCAAGA
    CAGCTAATGC ATAGTGGCCA TCCAAGTGAG AAGGAGATAA AAGCACAGCA AGATAAACTC
    AACACAAGAT GGAGCCAGTT CAGAGAACTG GTAGACAGGA AGAAAGATGC CCTGATCTCT
    GCCTTAAGTA TCCAGAATTA CCACCTTGAA TGTAATGAAA CCAAGTCCTG GATCAGGGAG
    AAAACCAAAG TGATTGAATC CACACAAGAT TTGGGTAACG ATCTTGCTGG AGTGATGGCT
    CTGCAGCGGA AGCTGACAGG CATGGAACGT GACCTGGTAG CCATTGAAGC TAAGCTCAGT
    GACCTGCAAA AGGAGGCAGA AAAGTTGGAA TCAGAGCATC CTGACCAGGC GCAAGCTATC
    CTTTCACGGC TTGCGGAAAT CAATGATGTA TGGGAAGAAA TGAAGACCAC CCTGAAAAAC
    CGGGAGGAGT CTCTTGGAGA GGCAAGCAAA CTGCAGCAGT TCCTGAGAGA TCTGGATGAC
    TTCCAGTCTT GGCTATCCAG AACACAGACT GCAATCGCCT CTGAAGACAT GCCGAACACA
    CTGACAGAGG CTGAGAAACT ACTCACTCAA CATGAGAATA TTAAGAACGA AATCAACAAT
    TACGAGGAAG ACTATCAGAA AATGAGGGAC ATGGGTGAGA TGGTGACACA AGGGCAGACT
    GATGCTCAGT ACATGTTCTT ACGCCAGCGC CTACAGGCTT TGGATACTGG ATGGAATGAG
    CTTCACAAAA TGTGGGAGAA CCGGCAAAAT CTTCTTTCCC AGTCTCATGC CTACCAGCTC
    TTTCTCAGAG ATACCAAGCA AGCAGAAGCA TTTCTTAACA ACCAGGAGTA TGTTTTGGCA
    CACACAGAAA TGCCCACTAC CTTGGAAGGA GCAGAAGCTG CTATTAAAAA ACAGGAGGAT
    TTCATGACCA CCATGGATGC GAATGAAGAA AAGATCAATG CAGTTGTAGA GACGGGCAGG
    AGGCTAGTTA GTGATGGGAA CATTAACTCT GACAAAATCC AGGAGAAAGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA CCGTGAAGCA GCCAGCGAGC TTCTGATGAG ACTGAAGGAT
    AACAGGGATC TTCAGAAGTT TCTTCAGGAT TGTCAAGAGC TCTCACTCTG GATCAATGAG
    AAGATGCTTA CAGCCCAGGA TATGTCTTAC GACGAGGCAA GAAACCTGCA CAGCAAATGG
    CTGAAGCATC AGGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT GGAGAAGATT
    GAAAAGGAGG GAATGCAGCT CATTGCAGAG AAACCAGAGA CTGAAGCTGT AGTGAAAGAA
    AAGCTAACAG GTCTTCATCA AATGTGGGAA GAGCTCGAAT CCACTACTCA GACCAAGGCT
    CAGCGTCTCT TTGATGCAAA CAAGGCGGAG CTTTTTACCC AGAGCTGTGC TGATTTGGAT
    AAGTGGCTGA ATGGTTTGGA GAGCCAAATT CAGTCAGATG ACTACGGAAA AGACCTCACC
    AGTGTCAACA TCCTATTGAA GAAGCAGCAG ATGCTAGAGA ATCAAATGGA TGTCCGTAAG
    AAGGAGATAG AAGAGCTACA AAGCCAGGCA CGGGCTCTAA GTCAAGAAGG GAAGAGTACA
    GATGAGGTAG ATGGTAAACG CCTTACCGTT GAAAAGAAAT TTTTGGAGTT GTTGGAACCT
    TTGAATGAAA GAAAAGCAAA CCTGCTGGCC TCCAAAGAAA TCCACCAGTT CAACCGGGAC
    GTGGAAGATG AAATTTTGTG GGTTGGAGAG AGGATGCCTA TAGCAACATC TACTGATCAC
    GGACACAACC TCCAGACTGT GCAGCTACTG ATAAAGAAAA ATCAGACACT TCAGAAAGAA
    ATCCAAGGAC ATCAGCCTCG TATTGATGAT ATTTTTGAGA GGAGTCAAAA CATTATCACA
    GACAGTAGCC CTAATGCTGA AGCAATTCAG CAGAGACTTG CAGACCTGAA GCAGCTGTGG
    AACCTCCTAA TTGAGGAGAC AGAGAAACGC CACAAACGCC TGGAGGAGTC TCACCGGGCA
    CAGCAGTACT ACTTTGATGC TGCCGAGGCT GAGGCCTGGA TGAGTGAGCA GGAGCTTTAC
    ATGATGTCAG AAGAGAAAGC CAAGGATGAA CAGAGTGCGG TATCCATGTT GAAGAAACAT
    CAGATTCTGG AACAGGCTGT GGAAGACTAT GCTGAGACTG TGCACCAGCT TTCAAAGACC
    AGCAGAACTT TGGTGGCAGA TAATCATCCT GAAAGTGAGC GTATCAGCAT GCGCCAGTCC
    AAAGTGGATA AGCTGTATGC AGGCCTGAAG GATCTGGCAG AAGAGAGAAG GGGCAAGCTG
    GATGAGAGAC ACAGGCTGTT CCAGCTGAAC AGAGAGGTGG ATGACCTGGA GCAGTGGATT
    GCTGAAAGGG AGGTGGTGGC AGGATCCCAT GAGCTGGGAC AGGATTATGA ACACGTAACG
    ATGCTACAGG AGCGATTCCG AGAATTTGCC CGAGACACTG GAAACATTGG ACAGGAGCGT
    GTTGATACCG TTAACCATCT GGCTGACGAG CTCATCAACT CTGGGCACTC AGATGCTGCC
    ACAATTGCAG AATGGAAGGA TGGTCTCAAT GAGGCCTGGG CTGATCTCCT GGAGCTCATT
    GACACAAGAA CACAAATTCT TGCAGCCTCT TACGAACTGC ACAAATTTTA CCACGATGCC
    AAGGAAATCT TAGGACGCAT TCAAGACAAA CACAAGAAAC TGCCAGAAGA GCTTGGTAGA
    GACCAAAACA CAGTGGAAAC ACTACAGAGA ATGCACACAA CATTTGAACA TGATATCCAA
    GCGCTCGGCA CACAGGTAAG ACAGCTCCAG GAGGATGCTG CACGCCTTCA GGCTGCCTAT
    GCTGGAGACA AAGCTGATGA CATCCAGAAG AGGGAAAATG AGGTCCTAGA AGCATGGAAG
    GCGCTGTTGG ATGCCTGTGA GGGCCGCAGG GTTAGGCTGG TGGACACAGG AGACAAATTC
    CGTTTCTTCA GCATGGTTCG GGATCTCATG CTTTGGATGG AAGATGTTAT CCGGCAGATC
    GAAGCCCAGG AAAAGCCAAG GGATGTTTCA TCTGTTGAAC TACTAATGAA CAATCACCAG
    GGTATCAAAG CGGAAATTGA TGCCCGAAAT GACAGCTTTA CTACCTGTAT TGAACTCGGC
    AAATCTCTTC TGGCAAGAAA ACACTATGCA TCAGAGGAGA TCAAGGAAAA GCTACTGCAG
    CTGACAGAGA AAAGAAAAGA AATGATTGAC AAGTGGGAAG ACAGATGGGA GTGGCTGAGA
    CTGATTTTGG AGGTGCATCA GTTTTCAAGA GACGCAAGTG TGGCTGAGGC CTGGCTGCTG
    GGACAGGAGC CCTACCTATC CAGTCGTGAA ATAGGTCAGA GTGTTGACGA AGTGGAAAAA
    TTAATTAAGC GGCATGAAGC ATTTGAGAAA TCTGCAGCTA CTTGGGATGA GAGATTTGCA
    GCGCTGGAAA GACTGACTAC GTTGGAACTG CTGGAAGTTC GTCGACAACA AGAGGAAGAA
    GAGAGGAAGA GAAAGCCACC TACTCCAGAG CCAAGCCCCA AAGTTGCAGA AGATGGAGGC
    TCCCAGCAGC AATGGGATGG GACAAAAGGA GAACAGGTTT CCCAAAATGG TTTGCCATCA
    GACCAGGAAT CTCCACGGGT GGCAGAAACT GCGGAAACAA ATGAAATGGT TAATGGAGCT
    GCAGAACAGA GGACAAGTTC GAAAGAGTCT AGTCCCGTTC CTTCTCCAAC AGCAGACCGC
    AAAGCCAAGG CTGCCATTCA GGCTCAGACT GCTGCTACCC TACCAGCCAA AACACAGGAG
    ATTCCTTCAG CTCAGATGGA GGGCTTCTTG CATCGTAAAC ATGAATGGGA GACACACAAC
    AAGAAAGCCT CAAGCAGGTC ATGGCATAAC GTGTACTGTG TCATAAACAA CCAAGAGATG
    GGCTTCTACA AGGATTCAAA AGCTGCTGCT TCTGGCATTC CCTATCACAA TGAGATTCCA
    GTCAGCTTGA AAGAAGCAGT CTGTGAAATA GCAGTCGATT ATAAAAAGAA AAAGCATGTG
    TTCAAGCTAA GATTGACTGA TGGCAATGAA TACCTCTTCC AAGCCAAAGA CGATGAGGAG
    ATGAACACGT GGATTCAGGC CATCACATCA GCTATCTCAT CTGACAAAAT CGAAGTGTCC
    CCGACAACTC AGAGCACTCC AGCCTCCAGC CGTGCCCAGA CCCTGCCTGC CAGCGTCACA
    ATAACCAGTG AGTCCAGTCC TGGCAAGAGA GAGAAAGACA AAGAGAAAGA CAAAGAGAAA
    AGATTCAGCC TTTTTGGTAA AAAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa33038
    Clone ID Related Accession (Same CDS sequence) XM_015283353.1
    Accession Version XM_015283353.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTAC AAAAATCAAC CAGTATGCCT GGGCCGTTGT CCCCAGGATA CGCAGCTCAG 
    GTGCCCTATA ACTATAATCA GTTAGAAGGA AGATTCAAGC AGTTACAAGA TGAACGTGAG
    GCAGTTCAGA AGAAGACTTT CACCAAGTGG GTTAATTCCC ACTTGGCACG TGTGTCATGC
    AGAATTACAG ATTTGTACAC CGACCTTCGC GATGGAAGGA TGCTCATCAA ACTCCTGGAA
    GTTCTTTCAG GAGAGAGACT TCCCAAACCA ACTAAGGGAA GGATGCGCAT CCATTGTTTG
    GAGAATGTTG ATAAAGCCCT GCAGTTCCTG AAGGAGCAAC GAGTGCACCT GGAAAACATG
    GGATCTCATG ATATTGTGGA TGGGAATCAC AGGCTGACTC TTGGCCTTAT TTGGACTATT
    ATCCTGAGAT TCCAGATCCA AGATATCAGT GTTGAAACTG AGGACAACAA GGAGAAGAAG
    TCTGCTAAGG ATGCACTGCT TTTGTGGTGC CAGATGAAGA CAGCTGGTTA CCCCAACGTC
    AACATTCACA ACTTTACAAC AAGCTGGAGG GATGGAATGG CTTTCAATGC ATTGATACAC
    AAGCACAGGC CTGATCTGAT TGATTTTGAC AAGCTGAAGA AATCAAATGC ACACTACAAT
    CTGCAGAATG CTTTTAACCT TGCTGAGCAA CACCTTGGTC TTACTAAGCT CCTGGACCCT
    GAGGATATCA GTGTTGATCA TCCCGATGAA AAGTCAATCA TCACCTATGT GGTGACATAT
    TACCACTACT TCTCTAAAAT GAAAGCTTTA GCTGTTGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGACAATG CCATTGAAAC AGAGAAAATG ATTGAAAAAT ATGAATCGCT GGCTTCTGAT
    CTTCTGGAGT GGATTGAGCA AACCATTATC ATCCTCAACA ACCGCAAATT TGCCAACTCG
    CTAGTTGGTG TGCAACAGCA GCTGCAGGCA TTCAACACCT ACCGCACAGT AGAGAAACCA
    CCCAAATTTA CAGAAAAAGG AAACCTGGAA GTGCTGCTTT TTACCATCCA AAGCAAAATG
    AGAGCCAACA ATCAGAAAGT ATACATGCCA AGGGAGGGCA AACTCATCTC TGACATAAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCTGAA CATGAAAGAG AACTGGCACT GCGAAATGAG
    CTCATCAGAC AAGAGAAACT GGAACAACTT GCACGCCGAT TTGATCGCAA GGCAGCTATG
    AGAGAAACTT GGCTGAGTGA AAATCAACGT CTGGTTTCTC AGGATAACTT TGGCTTTGAC
    CTTCCAGCAG TAGAGGCTGC AACGAAAAAG CACGAGGCCA TTGAAACAGA CATTGCAGCA
    TATGAAGAGC GAGTCCAGGC TGTGGTTGCA GTTGCAAAGG AGCTTGAGAC TGAAAATTAT
    CATGATATCA AACGTATCAC TGCAAGAAAG GACAATGTTA TACGCCTGTG GGAGTACCTC
    CTGGAGCTAC TCAGGGCACG AAGACAACGG CTGGAAATGA ATCTTGGCCT ACAAAAGATT
    TTCCAGGAAA TGCTATACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TCTGCTGTCA
    CAAGACTATG GCAAACATTT GTTAGGAGTC GAAGACCTGT TGCAGAAACA TGCTCTAGTG
    GAAGCTGACA TTGCTATTCA GGCTGAGAGA GTGAGAGGGG TCAATGCATC TGCTCAGAAG
    TTTGCCACTG ATGGAGAAGG TTACAAGCCA TGTGACCCAC AGGTTATTAG GGATCGCGTC
    GCTCACATGG AGTTCTGTTA CCAAGAGCTG TGCCAGCTTT CAGCTGAGCG CCGGGCCCGC
    TTGGAGGAGT CCCGGCGCCT CTGGAAATTC TTCTGGGAAA TGGCTGAAGA AGAGGGTTGG
    ATCAGGGAGA AGGAGCAGAT CCTTTCATCT GATGACTACG GGAAGGACCT GACCAGCGTC
    GTCCGTCTCA TGAGTAAACA TAAGGCATTT GAAGATGAAA TGAGTGGTCG CAGTGGCCAC
    TTTCAGCAGG CGATAAAAGA AGGTGAAGAC ATGATCGCGG AAGAGAATTT TGGGTCTGAG
    AAAATCAGAG AAAGAATTAA GGATATTCGG GAGCAGTGGG CTAACTTGGA ACAGTTATCT
    GCCATTAGGA AGAAACGTCT GGAGGAAGCC TCTCTCCTTC ACCAGTTCCA GGCTGATGCT
    GATGACATTG ATGCATGGAT GTTGGACATC CTCAAAATTG TCTCCAGCAA TGATGTTGGC
    CATGACGAAT ATTCCACTCA GTCCTTGGTC AAGAAACACA AGGATGTTGC TGAAGAGATT
    GCAAGCTATC GGCCAACCAT TGACTCGCTT CATGAACAGG CAAAGGCTCT ACCACAGGAG
    CATGCCGGGT CTCCAGATGT GCAAGGCAGG TTGTCTGGAA TAGAAGAAAG ATACAAAGAG
    GTTGCTGAGC TAACTCGGCT GCGTAAACAG GCTTTGCAGG ATACCCTTGC TCTTTATAAG
    ATGTTCAGTG AGGCAGATGC TTGTGAGCTT TGGATTGATG AGAAGGAGAA GTGGCTGAAT
    AATATGCAGA TTCCAGAGAA GCTGGAGGAC CTGGAAGTGA TCCAGCACAG ATTTGAAAGC
    TTAGAACCAG AAATGAACAA TCAGGCTTCC CGTGTTGCTG TAGTGAACCA AATTGCAAGA
    CAGCTAATGC ATAGTGGCCA TCCAAGTGAG AAGGAGATAA AAGCACAGCA AGATAAACTC
    AACACAAGAT GGAGCCAGTT CAGAGAACTG GTAGACAGGA AGAAAGATGC CCTGATCTCT
    GCCTTAAGTA TCCAGAATTA CCACCTTGAA TGTAATGAAA CCAAGTCCTG GATCAGGGAG
    AAAACCAAAG TGATTGAATC CACACAAGAT TTGGGTAACG ATCTTGCTGG AGTGATGGCT
    CTGCAGCGGA AGCTGACAGG CATGGAACGT GACCTGGTAG CCATTGAAGC TAAGCTCAGT
    GACCTGCAAA AGGAGGCAGA AAAGTTGGAA TCAGAGCATC CTGACCAGGC GCAAGCTATC
    CTTTCACGGC TTGCGGAAAT CAATGATGTA TGGGAAGAAA TGAAGACCAC CCTGAAAAAC
    CGGGAGGAGT CTCTTGGAGA GGCAAGCAAA CTGCAGCAGT TCCTGAGAGA TCTGGATGAC
    TTCCAGTCTT GGCTATCCAG AACACAGACT GCAATCGCCT CTGAAGACAT GCCGAACACA
    CTGACAGAGG CTGAGAAACT ACTCACTCAA CATGAGAATA TTAAGAACGA AATCAACAAT
    TACGAGGAAG ACTATCAGAA AATGAGGGAC ATGGGTGAGA TGGTGACACA AGGGCAGACT
    GATGCTCAGT ACATGTTCTT ACGCCAGCGC CTACAGGCTT TGGATACTGG ATGGAATGAG
    CTTCACAAAA TGTGGGAGAA CCGGCAAAAT CTTCTTTCCC AGTCTCATGC CTACCAGCTC
    TTTCTCAGAG ATACCAAGCA AGCAGAAGCA TTTCTTAACA ACCAGGAGTA TGTTTTGGCA
    CACACAGAAA TGCCCACTAC CTTGGAAGGA GCAGAAGCTG CTATTAAAAA ACAGGAGGAT
    TTCATGACCA CCATGGATGC GAATGAAGAA AAGATCAATG CAGTTGTAGA GACGGGCAGG
    AGGCTAGTTA GTGATGGGAA CATTAACTCT GACAAAATCC AGGAGAAAGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA CCGTGAAGCA GCCAGCGAGC TTCTGATGAG ACTGAAGGAT
    AACAGGGATC TTCAGAAGTT TCTTCAGGAT TGTCAAGAGC TCTCACTCTG GATCAATGAG
    AAGATGCTTA CAGCCCAGGA TATGTCTTAC GACGAGGCAA GAAACCTGCA CAGCAAATGG
    CTGAAGCATC AGGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT GGAGAAGATT
    GAAAAGGAGG GAATGCAGCT CATTGCAGAG AAACCAGAGA CTGAAGCTGT AGTGAAAGAA
    AAGCTAACAG GTCTTCATCA AATGTGGGAA GAGCTCGAAT CCACTACTCA GACCAAGGCT
    CAGCGTCTCT TTGATGCAAA CAAGGCGGAG CTTTTTACCC AGAGCTGTGC TGATTTGGAT
    AAGTGGCTGA ATGGTTTGGA GAGCCAAATT CAGTCAGATG ACTACGGAAA AGACCTCACC
    AGTGTCAACA TCCTATTGAA GAAGCAGCAG ATGCTAGAGA ATCAAATGGA TGTCCGTAAG
    AAGGAGATAG AAGAGCTACA AAGCCAGGCA CGGGCTCTAA GTCAAGAAGG GAAGAGTACA
    GATGAGGTAG ATGGTAAACG CCTTACCGTT GAAAAGAAAT TTTTGGAGTT GTTGGAACCT
    TTGAATGAAA GAAAAGCAAA CCTGCTGGCC TCCAAAGAAA TCCACCAGTT CAACCGGGAC
    GTGGAAGATG AAATTTTGTG GGTTGGAGAG AGGATGCCTA TAGCAACATC TACTGATCAC
    GGACACAACC TCCAGACTGT GCAGCTACTG ATAAAGAAAA ATCAGACACT TCAGAAAGAA
    ATCCAAGGAC ATCAGCCTCG TATTGATGAT ATTTTTGAGA GGAGTCAAAA CATTATCACA
    GACAGTAGCC CTAATGCTGA AGCAATTCAG CAGAGACTTG CAGACCTGAA GCAGCTGTGG
    AACCTCCTAA TTGAGGAGAC AGAGAAACGC CACAAACGCC TGGAGGAGTC TCACCGGGCA
    CAGCAGTACT ACTTTGATGC TGCCGAGGCT GAGGCCTGGA TGAGTGAGCA GGAGCTTTAC
    ATGATGTCAG AAGAGAAAGC CAAGGATGAA CAGAGTGCGG TATCCATGTT GAAGAAACAT
    CAGATTCTGG AACAGGCTGT GGAAGACTAT GCTGAGACTG TGCACCAGCT TTCAAAGACC
    AGCAGAACTT TGGTGGCAGA TAATCATCCT GAAAGTGAGC GTATCAGCAT GCGCCAGTCC
    AAAGTGGATA AGCTGTATGC AGGCCTGAAG GATCTGGCAG AAGAGAGAAG GGGCAAGCTG
    GATGAGAGAC ACAGGCTGTT CCAGCTGAAC AGAGAGGTGG ATGACCTGGA GCAGTGGATT
    GCTGAAAGGG AGGTGGTGGC AGGATCCCAT GAGCTGGGAC AGGATTATGA ACACGTAACG
    ATGCTACAGG AGCGATTCCG AGAATTTGCC CGAGACACTG GAAACATTGG ACAGGAGCGT
    GTTGATACCG TTAACCATCT GGCTGACGAG CTCATCAACT CTGGGCACTC AGATGCTGCC
    ACAATTGCAG AATGGAAGGA TGGTCTCAAT GAGGCCTGGG CTGATCTCCT GGAGCTCATT
    GACACAAGAA CACAAATTCT TGCAGCCTCT TACGAACTGC ACAAATTTTA CCACGATGCC
    AAGGAAATCT TAGGACGCAT TCAAGACAAA CACAAGAAAC TGCCAGAAGA GCTTGGTAGA
    GACCAAAACA CAGTGGAAAC ACTACAGAGA ATGCACACAA CATTTGAACA TGATATCCAA
    GCGCTCGGCA CACAGGTAAG ACAGCTCCAG GAGGATGCTG CACGCCTTCA GGCTGCCTAT
    GCTGGAGACA AAGCTGATGA CATCCAGAAG AGGGAAAATG AGGTCCTAGA AGCATGGAAG
    GCGCTGTTGG ATGCCTGTGA GGGCCGCAGG GTTAGGCTGG TGGACACAGG AGACAAATTC
    CGTTTCTTCA GCATGGTTCG GGATCTCATG CTTTGGATGG AAGATGTTAT CCGGCAGATC
    GAAGCCCAGG AAAAGCCAAG GGATGTTTCA TCTGTTGAAC TACTAATGAA CAATCACCAG
    GGTATCAAAG CGGAAATTGA TGCCCGAAAT GACAGCTTTA CTACCTGTAT TGAACTCGGC
    AAATCTCTTC TGGCAAGAAA ACACTATGCA TCAGAGGAGA TCAAGGAAAA GCTACTGCAG
    CTGACAGAGA AAAGAAAAGA AATGATTGAC AAGTGGGAAG ACAGATGGGA GTGGCTGAGA
    CTGATTTTGG AGGTGCATCA GTTTTCAAGA GACGCAAGTG TGGCTGAGGC CTGGCTGCTG
    GGACAGGAGC CCTACCTATC CAGTCGTGAA ATAGGTCAGA GTGTTGACGA AGTGGAAAAA
    TTAATTAAGC GGCATGAAGC ATTTGAGAAA TCTGCAGCTA CTTGGGATGA GAGATTTGCA
    GCGCTGGAAA GACTGACTAC GTTGGAACTG CTGGAAGTTC GTCGACAACA AGAGGAAGAA
    GAGAGGAAGA GAAAGCCACC TACTCCAGAG CCAAGCCCCA AAGTTGCAGA AGATGGAGGC
    TCCCAGCAGC AATGGGATGG GACAAAAGGA GAACAGGTTT CCCAAAATGG TTTGCCATCA
    GACCAGGAAT CTCCACGGGT TAGTTACCGC TCCCAAACCT ACCAAAACTA CAAAAACTTT
    ATTAGCAGAC GGACAGCCAA TGACCGATCG TGGTCTGGAC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138839.1
    CDS1..6465
    Translation

    Target ORF information:

    RefSeq Version XM_015283353.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015283353.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTAC AAAAATCAAC CAGTATGCCT GGGCCGTTGT CCCCAGGATA CGCAGCTCAG 
    GTGCCCTATA ACTATAATCA GTTAGAAGGA AGATTCAAGC AGTTACAAGA TGAACGTGAG
    GCAGTTCAGA AGAAGACTTT CACCAAGTGG GTTAATTCCC ACTTGGCACG TGTGTCATGC
    AGAATTACAG ATTTGTACAC CGACCTTCGC GATGGAAGGA TGCTCATCAA ACTCCTGGAA
    GTTCTTTCAG GAGAGAGACT TCCCAAACCA ACTAAGGGAA GGATGCGCAT CCATTGTTTG
    GAGAATGTTG ATAAAGCCCT GCAGTTCCTG AAGGAGCAAC GAGTGCACCT GGAAAACATG
    GGATCTCATG ATATTGTGGA TGGGAATCAC AGGCTGACTC TTGGCCTTAT TTGGACTATT
    ATCCTGAGAT TCCAGATCCA AGATATCAGT GTTGAAACTG AGGACAACAA GGAGAAGAAG
    TCTGCTAAGG ATGCACTGCT TTTGTGGTGC CAGATGAAGA CAGCTGGTTA CCCCAACGTC
    AACATTCACA ACTTTACAAC AAGCTGGAGG GATGGAATGG CTTTCAATGC ATTGATACAC
    AAGCACAGGC CTGATCTGAT TGATTTTGAC AAGCTGAAGA AATCAAATGC ACACTACAAT
    CTGCAGAATG CTTTTAACCT TGCTGAGCAA CACCTTGGTC TTACTAAGCT CCTGGACCCT
    GAGGATATCA GTGTTGATCA TCCCGATGAA AAGTCAATCA TCACCTATGT GGTGACATAT
    TACCACTACT TCTCTAAAAT GAAAGCTTTA GCTGTTGAAG GAAAGCGCAT TGGAAAGGTG
    CTTGACAATG CCATTGAAAC AGAGAAAATG ATTGAAAAAT ATGAATCGCT GGCTTCTGAT
    CTTCTGGAGT GGATTGAGCA AACCATTATC ATCCTCAACA ACCGCAAATT TGCCAACTCG
    CTAGTTGGTG TGCAACAGCA GCTGCAGGCA TTCAACACCT ACCGCACAGT AGAGAAACCA
    CCCAAATTTA CAGAAAAAGG AAACCTGGAA GTGCTGCTTT TTACCATCCA AAGCAAAATG
    AGAGCCAACA ATCAGAAAGT ATACATGCCA AGGGAGGGCA AACTCATCTC TGACATAAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCTGAA CATGAAAGAG AACTGGCACT GCGAAATGAG
    CTCATCAGAC AAGAGAAACT GGAACAACTT GCACGCCGAT TTGATCGCAA GGCAGCTATG
    AGAGAAACTT GGCTGAGTGA AAATCAACGT CTGGTTTCTC AGGATAACTT TGGCTTTGAC
    CTTCCAGCAG TAGAGGCTGC AACGAAAAAG CACGAGGCCA TTGAAACAGA CATTGCAGCA
    TATGAAGAGC GAGTCCAGGC TGTGGTTGCA GTTGCAAAGG AGCTTGAGAC TGAAAATTAT
    CATGATATCA AACGTATCAC TGCAAGAAAG GACAATGTTA TACGCCTGTG GGAGTACCTC
    CTGGAGCTAC TCAGGGCACG AAGACAACGG CTGGAAATGA ATCTTGGCCT ACAAAAGATT
    TTCCAGGAAA TGCTATACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TCTGCTGTCA
    CAAGACTATG GCAAACATTT GTTAGGAGTC GAAGACCTGT TGCAGAAACA TGCTCTAGTG
    GAAGCTGACA TTGCTATTCA GGCTGAGAGA GTGAGAGGGG TCAATGCATC TGCTCAGAAG
    TTTGCCACTG ATGGAGAAGG TTACAAGCCA TGTGACCCAC AGGTTATTAG GGATCGCGTC
    GCTCACATGG AGTTCTGTTA CCAAGAGCTG TGCCAGCTTT CAGCTGAGCG CCGGGCCCGC
    TTGGAGGAGT CCCGGCGCCT CTGGAAATTC TTCTGGGAAA TGGCTGAAGA AGAGGGTTGG
    ATCAGGGAGA AGGAGCAGAT CCTTTCATCT GATGACTACG GGAAGGACCT GACCAGCGTC
    GTCCGTCTCA TGAGTAAACA TAAGGCATTT GAAGATGAAA TGAGTGGTCG CAGTGGCCAC
    TTTCAGCAGG CGATAAAAGA AGGTGAAGAC ATGATCGCGG AAGAGAATTT TGGGTCTGAG
    AAAATCAGAG AAAGAATTAA GGATATTCGG GAGCAGTGGG CTAACTTGGA ACAGTTATCT
    GCCATTAGGA AGAAACGTCT GGAGGAAGCC TCTCTCCTTC ACCAGTTCCA GGCTGATGCT
    GATGACATTG ATGCATGGAT GTTGGACATC CTCAAAATTG TCTCCAGCAA TGATGTTGGC
    CATGACGAAT ATTCCACTCA GTCCTTGGTC AAGAAACACA AGGATGTTGC TGAAGAGATT
    GCAAGCTATC GGCCAACCAT TGACTCGCTT CATGAACAGG CAAAGGCTCT ACCACAGGAG
    CATGCCGGGT CTCCAGATGT GCAAGGCAGG TTGTCTGGAA TAGAAGAAAG ATACAAAGAG
    GTTGCTGAGC TAACTCGGCT GCGTAAACAG GCTTTGCAGG ATACCCTTGC TCTTTATAAG
    ATGTTCAGTG AGGCAGATGC TTGTGAGCTT TGGATTGATG AGAAGGAGAA GTGGCTGAAT
    AATATGCAGA TTCCAGAGAA GCTGGAGGAC CTGGAAGTGA TCCAGCACAG ATTTGAAAGC
    TTAGAACCAG AAATGAACAA TCAGGCTTCC CGTGTTGCTG TAGTGAACCA AATTGCAAGA
    CAGCTAATGC ATAGTGGCCA TCCAAGTGAG AAGGAGATAA AAGCACAGCA AGATAAACTC
    AACACAAGAT GGAGCCAGTT CAGAGAACTG GTAGACAGGA AGAAAGATGC CCTGATCTCT
    GCCTTAAGTA TCCAGAATTA CCACCTTGAA TGTAATGAAA CCAAGTCCTG GATCAGGGAG
    AAAACCAAAG TGATTGAATC CACACAAGAT TTGGGTAACG ATCTTGCTGG AGTGATGGCT
    CTGCAGCGGA AGCTGACAGG CATGGAACGT GACCTGGTAG CCATTGAAGC TAAGCTCAGT
    GACCTGCAAA AGGAGGCAGA AAAGTTGGAA TCAGAGCATC CTGACCAGGC GCAAGCTATC
    CTTTCACGGC TTGCGGAAAT CAATGATGTA TGGGAAGAAA TGAAGACCAC CCTGAAAAAC
    CGGGAGGAGT CTCTTGGAGA GGCAAGCAAA CTGCAGCAGT TCCTGAGAGA TCTGGATGAC
    TTCCAGTCTT GGCTATCCAG AACACAGACT GCAATCGCCT CTGAAGACAT GCCGAACACA
    CTGACAGAGG CTGAGAAACT ACTCACTCAA CATGAGAATA TTAAGAACGA AATCAACAAT
    TACGAGGAAG ACTATCAGAA AATGAGGGAC ATGGGTGAGA TGGTGACACA AGGGCAGACT
    GATGCTCAGT ACATGTTCTT ACGCCAGCGC CTACAGGCTT TGGATACTGG ATGGAATGAG
    CTTCACAAAA TGTGGGAGAA CCGGCAAAAT CTTCTTTCCC AGTCTCATGC CTACCAGCTC
    TTTCTCAGAG ATACCAAGCA AGCAGAAGCA TTTCTTAACA ACCAGGAGTA TGTTTTGGCA
    CACACAGAAA TGCCCACTAC CTTGGAAGGA GCAGAAGCTG CTATTAAAAA ACAGGAGGAT
    TTCATGACCA CCATGGATGC GAATGAAGAA AAGATCAATG CAGTTGTAGA GACGGGCAGG
    AGGCTAGTTA GTGATGGGAA CATTAACTCT GACAAAATCC AGGAGAAAGT GGACTCTATT
    GATGACAGAC ATAGGAAGAA CCGTGAAGCA GCCAGCGAGC TTCTGATGAG ACTGAAGGAT
    AACAGGGATC TTCAGAAGTT TCTTCAGGAT TGTCAAGAGC TCTCACTCTG GATCAATGAG
    AAGATGCTTA CAGCCCAGGA TATGTCTTAC GACGAGGCAA GAAACCTGCA CAGCAAATGG
    CTGAAGCATC AGGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT GGAGAAGATT
    GAAAAGGAGG GAATGCAGCT CATTGCAGAG AAACCAGAGA CTGAAGCTGT AGTGAAAGAA
    AAGCTAACAG GTCTTCATCA AATGTGGGAA GAGCTCGAAT CCACTACTCA GACCAAGGCT
    CAGCGTCTCT TTGATGCAAA CAAGGCGGAG CTTTTTACCC AGAGCTGTGC TGATTTGGAT
    AAGTGGCTGA ATGGTTTGGA GAGCCAAATT CAGTCAGATG ACTACGGAAA AGACCTCACC
    AGTGTCAACA TCCTATTGAA GAAGCAGCAG ATGCTAGAGA ATCAAATGGA TGTCCGTAAG
    AAGGAGATAG AAGAGCTACA AAGCCAGGCA CGGGCTCTAA GTCAAGAAGG GAAGAGTACA
    GATGAGGTAG ATGGTAAACG CCTTACCGTT GAAAAGAAAT TTTTGGAGTT GTTGGAACCT
    TTGAATGAAA GAAAAGCAAA CCTGCTGGCC TCCAAAGAAA TCCACCAGTT CAACCGGGAC
    GTGGAAGATG AAATTTTGTG GGTTGGAGAG AGGATGCCTA TAGCAACATC TACTGATCAC
    GGACACAACC TCCAGACTGT GCAGCTACTG ATAAAGAAAA ATCAGACACT TCAGAAAGAA
    ATCCAAGGAC ATCAGCCTCG TATTGATGAT ATTTTTGAGA GGAGTCAAAA CATTATCACA
    GACAGTAGCC CTAATGCTGA AGCAATTCAG CAGAGACTTG CAGACCTGAA GCAGCTGTGG
    AACCTCCTAA TTGAGGAGAC AGAGAAACGC CACAAACGCC TGGAGGAGTC TCACCGGGCA
    CAGCAGTACT ACTTTGATGC TGCCGAGGCT GAGGCCTGGA TGAGTGAGCA GGAGCTTTAC
    ATGATGTCAG AAGAGAAAGC CAAGGATGAA CAGAGTGCGG TATCCATGTT GAAGAAACAT
    CAGATTCTGG AACAGGCTGT GGAAGACTAT GCTGAGACTG TGCACCAGCT TTCAAAGACC
    AGCAGAACTT TGGTGGCAGA TAATCATCCT GAAAGTGAGC GTATCAGCAT GCGCCAGTCC
    AAAGTGGATA AGCTGTATGC AGGCCTGAAG GATCTGGCAG AAGAGAGAAG GGGCAAGCTG
    GATGAGAGAC ACAGGCTGTT CCAGCTGAAC AGAGAGGTGG ATGACCTGGA GCAGTGGATT
    GCTGAAAGGG AGGTGGTGGC AGGATCCCAT GAGCTGGGAC AGGATTATGA ACACGTAACG
    ATGCTACAGG AGCGATTCCG AGAATTTGCC CGAGACACTG GAAACATTGG ACAGGAGCGT
    GTTGATACCG TTAACCATCT GGCTGACGAG CTCATCAACT CTGGGCACTC AGATGCTGCC
    ACAATTGCAG AATGGAAGGA TGGTCTCAAT GAGGCCTGGG CTGATCTCCT GGAGCTCATT
    GACACAAGAA CACAAATTCT TGCAGCCTCT TACGAACTGC ACAAATTTTA CCACGATGCC
    AAGGAAATCT TAGGACGCAT TCAAGACAAA CACAAGAAAC TGCCAGAAGA GCTTGGTAGA
    GACCAAAACA CAGTGGAAAC ACTACAGAGA ATGCACACAA CATTTGAACA TGATATCCAA
    GCGCTCGGCA CACAGGTAAG ACAGCTCCAG GAGGATGCTG CACGCCTTCA GGCTGCCTAT
    GCTGGAGACA AAGCTGATGA CATCCAGAAG AGGGAAAATG AGGTCCTAGA AGCATGGAAG
    GCGCTGTTGG ATGCCTGTGA GGGCCGCAGG GTTAGGCTGG TGGACACAGG AGACAAATTC
    CGTTTCTTCA GCATGGTTCG GGATCTCATG CTTTGGATGG AAGATGTTAT CCGGCAGATC
    GAAGCCCAGG AAAAGCCAAG GGATGTTTCA TCTGTTGAAC TACTAATGAA CAATCACCAG
    GGTATCAAAG CGGAAATTGA TGCCCGAAAT GACAGCTTTA CTACCTGTAT TGAACTCGGC
    AAATCTCTTC TGGCAAGAAA ACACTATGCA TCAGAGGAGA TCAAGGAAAA GCTACTGCAG
    CTGACAGAGA AAAGAAAAGA AATGATTGAC AAGTGGGAAG ACAGATGGGA GTGGCTGAGA
    CTGATTTTGG AGGTGCATCA GTTTTCAAGA GACGCAAGTG TGGCTGAGGC CTGGCTGCTG
    GGACAGGAGC CCTACCTATC CAGTCGTGAA ATAGGTCAGA GTGTTGACGA AGTGGAAAAA
    TTAATTAAGC GGCATGAAGC ATTTGAGAAA TCTGCAGCTA CTTGGGATGA GAGATTTGCA
    GCGCTGGAAA GACTGACTAC GTTGGAACTG CTGGAAGTTC GTCGACAACA AGAGGAAGAA
    GAGAGGAAGA GAAAGCCACC TACTCCAGAG CCAAGCCCCA AAGTTGCAGA AGATGGAGGC
    TCCCAGCAGC AATGGGATGG GACAAAAGGA GAACAGGTTT CCCAAAATGG TTTGCCATCA
    GACCAGGAAT CTCCACGGGT TAGTTACCGC TCCCAAACCT ACCAAAACTA CAAAAACTTT
    ATTAGCAGAC GGACAGCCAA TGACCGATCG TGGTCTGGAC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00878
    Clone ID Related Accession (Same CDS sequence) XM_015283350.1 , XM_015283350.2 , NM_001199425.2 , NM_001199425.1
    Accession Version NM_001199425.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-19
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000009.1. On Dec 20, 2018 this sequence version replaced NM_001199425.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186354.1
    CDS53..7141
    Translation

    Target ORF information:

    RefSeq Version NM_001199425.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199425.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00878
    Clone ID Related Accession (Same CDS sequence) XM_015283350.1 , XM_015283350.2 , NM_001199425.2 , NM_001199425.1
    Accession Version NM_001199425.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-07-01
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN04000266.1. On Dec 8, 2010 this sequence version replaced XM_419291.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2201357, SAMEA2201358 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001186354.1
    CDS53..7141
    Translation

    Target ORF information:

    RefSeq Version NM_001199425.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001199425.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00878
    Clone ID Related Accession (Same CDS sequence) XM_015283350.1 , XM_015283350.2 , NM_001199425.2 , NM_001199425.1
    Accession Version XM_015283350.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455831.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138836.1
    CDS135..7223
    Translation

    Target ORF information:

    RefSeq Version XM_015283350.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015283350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa00878
    Clone ID Related Accession (Same CDS sequence) XM_015283350.1 , XM_015283350.2 , NM_001199425.2 , NM_001199425.1
    Accession Version XM_015283350.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015138836.1
    CDS138..7226
    Translation

    Target ORF information:

    RefSeq Version XM_015283350.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015283350.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACAA CAGTGGCGAC AGATTATGAC AACATTGAAA TCCAGCAGCA GTACAGTGAT 
    GTCAACAACC GCTGGGATGT CGATGACTGG GACAATGAAA ATAGTTCTGC TCGACTGTTT
    GAACGATCTC GCATCAAGGC CCTAGCTGAT GAACGTGAGG CAGTTCAGAA GAAGACTTTC
    ACCAAGTGGG TTAATTCCCA CTTGGCACGT GTGTCATGCA GAATTACAGA TTTGTACACC
    GACCTTCGCG ATGGAAGGAT GCTCATCAAA CTCCTGGAAG TTCTTTCAGG AGAGAGACTT
    CCCAAACCAA CTAAGGGAAG GATGCGCATC CATTGTTTGG AGAATGTTGA TAAAGCCCTG
    CAGTTCCTGA AGGAGCAACG AGTGCACCTG GAAAACATGG GATCTCATGA TATTGTGGAT
    GGGAATCACA GGCTGACTCT TGGCCTTATT TGGACTATTA TCCTGAGATT CCAGATCCAA
    GATATCAGTG TTGAAACTGA GGACAACAAG GAGAAGAAGT CTGCTAAGGA TGCACTGCTT
    TTGTGGTGCC AGATGAAGAC AGCTGGTTAC CCCAACGTCA ACATTCACAA CTTTACAACA
    AGCTGGAGGG ATGGAATGGC TTTCAATGCA TTGATACACA AGCACAGGCC TGATCTGATT
    GATTTTGACA AGCTGAAGAA ATCAAATGCA CACTACAATC TGCAGAATGC TTTTAACCTT
    GCTGAGCAAC ACCTTGGTCT TACTAAGCTC CTGGACCCTG AGGATATCAG TGTTGATCAT
    CCCGATGAAA AGTCAATCAT CACCTATGTG GTGACATATT ACCACTACTT CTCTAAAATG
    AAAGCTTTAG CTGTTGAAGG AAAGCGCATT GGAAAGGTGC TTGACAATGC CATTGAAACA
    GAGAAAATGA TTGAAAAATA TGAATCGCTG GCTTCTGATC TTCTGGAGTG GATTGAGCAA
    ACCATTATCA TCCTCAACAA CCGCAAATTT GCCAACTCGC TAGTTGGTGT GCAACAGCAG
    CTGCAGGCAT TCAACACCTA CCGCACAGTA GAGAAACCAC CCAAATTTAC AGAAAAAGGA
    AACCTGGAAG TGCTGCTTTT TACCATCCAA AGCAAAATGA GAGCCAACAA TCAGAAAGTA
    TACATGCCAA GGGAGGGCAA ACTCATCTCT GACATAAACA AGGCCTGGGA AAGACTGGAA
    AAAGCTGAAC ATGAAAGAGA ACTGGCACTG CGAAATGAGC TCATCAGACA AGAGAAACTG
    GAACAACTTG CACGCCGATT TGATCGCAAG GCAGCTATGA GAGAAACTTG GCTGAGTGAA
    AATCAACGTC TGGTTTCTCA GGATAACTTT GGCTTTGACC TTCCAGCAGT AGAGGCTGCA
    ACGAAAAAGC ACGAGGCCAT TGAAACAGAC ATTGCAGCAT ATGAAGAGCG AGTCCAGGCT
    GTGGTTGCAG TTGCAAAGGA GCTTGAGACT GAAAATTATC ATGATATCAA ACGTATCACT
    GCAAGAAAGG ACAATGTTAT ACGCCTGTGG GAGTACCTCC TGGAGCTACT CAGGGCACGA
    AGACAACGGC TGGAAATGAA TCTTGGCCTA CAAAAGATTT TCCAGGAAAT GCTATACATT
    ATGGACTGGA TGGATGAGAT GAAGGTGCTT CTGCTGTCAC AAGACTATGG CAAACATTTG
    TTAGGAGTCG AAGACCTGTT GCAGAAACAT GCTCTAGTGG AAGCTGACAT TGCTATTCAG
    GCTGAGAGAG TGAGAGGGGT CAATGCATCT GCTCAGAAGT TTGCCACTGA TGGAGAAGGT
    TACAAGCCAT GTGACCCACA GGTTATTAGG GATCGCGTCG CTCACATGGA GTTCTGTTAC
    CAAGAGCTGT GCCAGCTTTC AGCTGAGCGC CGGGCCCGCT TGGAGGAGTC CCGGCGCCTC
    TGGAAATTCT TCTGGGAAAT GGCTGAAGAA GAGGGTTGGA TCAGGGAGAA GGAGCAGATC
    CTTTCATCTG ATGACTACGG GAAGGACCTG ACCAGCGTCG TCCGTCTCAT GAGTAAACAT
    AAGGCATTTG AAGATGAAAT GAGTGGTCGC AGTGGCCACT TTCAGCAGGC GATAAAAGAA
    GGTGAAGACA TGATCGCGGA AGAGAATTTT GGGTCTGAGA AAATCAGAGA AAGAATTAAG
    GATATTCGGG AGCAGTGGGC TAACTTGGAA CAGTTATCTG CCATTAGGAA GAAACGTCTG
    GAGGAAGCCT CTCTCCTTCA CCAGTTCCAG GCTGATGCTG ATGACATTGA TGCATGGATG
    TTGGACATCC TCAAAATTGT CTCCAGCAAT GATGTTGGCC ATGACGAATA TTCCACTCAG
    TCCTTGGTCA AGAAACACAA GGATGTTGCT GAAGAGATTG CAAGCTATCG GCCAACCATT
    GACTCGCTTC ATGAACAGGC AAAGGCTCTA CCACAGGAGC ATGCCGGGTC TCCAGATGTG
    CAAGGCAGGT TGTCTGGAAT AGAAGAAAGA TACAAAGAGG TTGCTGAGCT AACTCGGCTG
    CGTAAACAGG CTTTGCAGGA TACCCTTGCT CTTTATAAGA TGTTCAGTGA GGCAGATGCT
    TGTGAGCTTT GGATTGATGA GAAGGAGAAG TGGCTGAATA ATATGCAGAT TCCAGAGAAG
    CTGGAGGACC TGGAAGTGAT CCAGCACAGA TTTGAAAGCT TAGAACCAGA AATGAACAAT
    CAGGCTTCCC GTGTTGCTGT AGTGAACCAA ATTGCAAGAC AGCTAATGCA TAGTGGCCAT
    CCAAGTGAGA AGGAGATAAA AGCACAGCAA GATAAACTCA ACACAAGATG GAGCCAGTTC
    AGAGAACTGG TAGACAGGAA GAAAGATGCC CTGATCTCTG CCTTAAGTAT CCAGAATTAC
    CACCTTGAAT GTAATGAAAC CAAGTCCTGG ATCAGGGAGA AAACCAAAGT GATTGAATCC
    ACACAAGATT TGGGTAACGA TCTTGCTGGA GTGATGGCTC TGCAGCGGAA GCTGACAGGC
    ATGGAACGTG ACCTGGTAGC CATTGAAGCT AAGCTCAGTG ACCTGCAAAA GGAGGCAGAA
    AAGTTGGAAT CAGAGCATCC TGACCAGGCG CAAGCTATCC TTTCACGGCT TGCGGAAATC
    AATGATGTAT GGGAAGAAAT GAAGACCACC CTGAAAAACC GGGAGGAGTC TCTTGGAGAG
    GCAAGCAAAC TGCAGCAGTT CCTGAGAGAT CTGGATGACT TCCAGTCTTG GCTATCCAGA
    ACACAGACTG CAATCGCCTC TGAAGACATG CCGAACACAC TGACAGAGGC TGAGAAACTA
    CTCACTCAAC ATGAGAATAT TAAGAACGAA ATCAACAATT ACGAGGAAGA CTATCAGAAA
    ATGAGGGACA TGGGTGAGAT GGTGACACAA GGGCAGACTG ATGCTCAGTA CATGTTCTTA
    CGCCAGCGCC TACAGGCTTT GGATACTGGA TGGAATGAGC TTCACAAAAT GTGGGAGAAC
    CGGCAAAATC TTCTTTCCCA GTCTCATGCC TACCAGCTCT TTCTCAGAGA TACCAAGCAA
    GCAGAAGCAT TTCTTAACAA CCAGGAGTAT GTTTTGGCAC ACACAGAAAT GCCCACTACC
    TTGGAAGGAG CAGAAGCTGC TATTAAAAAA CAGGAGGATT TCATGACCAC CATGGATGCG
    AATGAAGAAA AGATCAATGC AGTTGTAGAG ACGGGCAGGA GGCTAGTTAG TGATGGGAAC
    ATTAACTCTG ACAAAATCCA GGAGAAAGTG GACTCTATTG ATGACAGACA TAGGAAGAAC
    CGTGAAGCAG CCAGCGAGCT TCTGATGAGA CTGAAGGATA ACAGGGATCT TCAGAAGTTT
    CTTCAGGATT GTCAAGAGCT CTCACTCTGG ATCAATGAGA AGATGCTTAC AGCCCAGGAT
    ATGTCTTACG ACGAGGCAAG AAACCTGCAC AGCAAATGGC TGAAGCATCA GGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTG GAGAAGATTG AAAAGGAGGG AATGCAGCTC
    ATTGCAGAGA AACCAGAGAC TGAAGCTGTA GTGAAAGAAA AGCTAACAGG TCTTCATCAA
    ATGTGGGAAG AGCTCGAATC CACTACTCAG ACCAAGGCTC AGCGTCTCTT TGATGCAAAC
    AAGGCGGAGC TTTTTACCCA GAGCTGTGCT GATTTGGATA AGTGGCTGAA TGGTTTGGAG
    AGCCAAATTC AGTCAGATGA CTACGGAAAA GACCTCACCA GTGTCAACAT CCTATTGAAG
    AAGCAGCAGA TGCTAGAGAA TCAAATGGAT GTCCGTAAGA AGGAGATAGA AGAGCTACAA
    AGCCAGGCAC GGGCTCTAAG TCAAGAAGGG AAGAGTACAG ATGAGGTAGA TGGTAAACGC
    CTTACCGTTG AAAAGAAATT TTTGGAGTTG TTGGAACCTT TGAATGAAAG AAAAGCAAAC
    CTGCTGGCCT CCAAAGAAAT CCACCAGTTC AACCGGGACG TGGAAGATGA AATTTTGTGG
    GTTGGAGAGA GGATGCCTAT AGCAACATCT ACTGATCACG GACACAACCT CCAGACTGTG
    CAGCTACTGA TAAAGAAAAA TCAGACACTT CAGAAAGAAA TCCAAGGACA TCAGCCTCGT
    ATTGATGATA TTTTTGAGAG GAGTCAAAAC ATTATCACAG ACAGTAGCCC TAATGCTGAA
    GCAATTCAGC AGAGACTTGC AGACCTGAAG CAGCTGTGGA ACCTCCTAAT TGAGGAGACA
    GAGAAACGCC ACAAACGCCT GGAGGAGTCT CACCGGGCAC AGCAGTACTA CTTTGATGCT
    GCCGAGGCTG AGGCCTGGAT GAGTGAGCAG GAGCTTTACA TGATGTCAGA AGAGAAAGCC
    AAGGATGAAC AGAGTGCGGT ATCCATGTTG AAGAAACATC AGATTCTGGA ACAGGCTGTG
    GAAGACTATG CTGAGACTGT GCACCAGCTT TCAAAGACCA GCAGAACTTT GGTGGCAGAT
    AATCATCCTG AAAGTGAGCG TATCAGCATG CGCCAGTCCA AAGTGGATAA GCTGTATGCA
    GGCCTGAAGG ATCTGGCAGA AGAGAGAAGG GGCAAGCTGG ATGAGAGACA CAGGCTGTTC
    CAGCTGAACA GAGAGGTGGA TGACCTGGAG CAGTGGATTG CTGAAAGGGA GGTGGTGGCA
    GGATCCCATG AGCTGGGACA GGATTATGAA CACGTAACGA TGCTACAGGA GCGATTCCGA
    GAATTTGCCC GAGACACTGG AAACATTGGA CAGGAGCGTG TTGATACCGT TAACCATCTG
    GCTGACGAGC TCATCAACTC TGGGCACTCA GATGCTGCCA CAATTGCAGA ATGGAAGGAT
    GGTCTCAATG AGGCCTGGGC TGATCTCCTG GAGCTCATTG ACACAAGAAC ACAAATTCTT
    GCAGCCTCTT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAAATCTT AGGACGCATT
    CAAGACAAAC ACAAGAAACT GCCAGAAGAG CTTGGTAGAG ACCAAAACAC AGTGGAAACA
    CTACAGAGAA TGCACACAAC ATTTGAACAT GATATCCAAG CGCTCGGCAC ACAGGTAAGA
    CAGCTCCAGG AGGATGCTGC ACGCCTTCAG GCTGCCTATG CTGGAGACAA AGCTGATGAC
    ATCCAGAAGA GGGAAAATGA GGTCCTAGAA GCATGGAAGG CGCTGTTGGA TGCCTGTGAG
    GGCCGCAGGG TTAGGCTGGT GGACACAGGA GACAAATTCC GTTTCTTCAG CATGGTTCGG
    GATCTCATGC TTTGGATGGA AGATGTTATC CGGCAGATCG AAGCCCAGGA AAAGCCAAGG
    GATGTTTCAT CTGTTGAACT ACTAATGAAC AATCACCAGG GTATCAAAGC GGAAATTGAT
    GCCCGAAATG ACAGCTTTAC TACCTGTATT GAACTCGGCA AATCTCTTCT GGCAAGAAAA
    CACTATGCAT CAGAGGAGAT CAAGGAAAAG CTACTGCAGC TGACAGAGAA AAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CAGATGGGAG TGGCTGAGAC TGATTTTGGA GGTGCATCAG
    TTTTCAAGAG ACGCAAGTGT GGCTGAGGCC TGGCTGCTGG GACAGGAGCC CTACCTATCC
    AGTCGTGAAA TAGGTCAGAG TGTTGACGAA GTGGAAAAAT TAATTAAGCG GCATGAAGCA
    TTTGAGAAAT CTGCAGCTAC TTGGGATGAG AGATTTGCAG CGCTGGAAAG ACTGACTACG
    TTGGAACTGC TGGAAGTTCG TCGACAACAA GAGGAAGAAG AGAGGAAGAG AAAGCCACCT
    ACTCCAGAGC CAAGCCCCAA AGTTGCAGAA GATGGAGGCT CCCAGCAGCA ATGGGATGGG
    ACAAAAGGAG AACAGGTTTC CCAAAATGGT TTGCCATCAG ACCAGGAATC TCCACGGGTG
    GCAGAAACTG CGGAAACAAA TGAAATGGTT AATGGAGCTG CAGAACAGAG GACAAGTTCG
    AAAGAGTCTA GTCCCGTTCC TTCTCCAACA GCAGACCGCA AAGCCAAGGC TGCCATTCAG
    GCTCAGACTG CTGCTACCCT ACCAGCCAAA ACACAGGAGA TTCCTTCAGC TCAGATGGAG
    GGCTTCTTGC ATCGTAAACA TGAATGGGAG ACACACAACA AGAAAGCCTC AAGCAGGTCA
    TGGCATAACG TGTACTGTGT CATAAACAAC CAAGAGATGG GCTTCTACAA GGATTCAAAA
    GCTGCTGCTT CTGGCATTCC CTATCACAAT GAGATTCCAG TCAGCTTGAA AGAAGCAGTC
    TGTGAAATAG CAGTCGATTA TAAAAAGAAA AAGCATGTGT TCAAGCTAAG ATTGACTGAT
    GGCAATGAAT ACCTCTTCCA AGCCAAAGAC GATGAGGAGA TGAACACGTG GATTCAGGCC
    ATCACATCAG CTATCTCATC TGACAAAATC GAAGTGTCCC CGACAACTCA GAGCACTCCA
    GCCTCCAGCC GTGCCCAGAC CCTGCCTGCC AGCGTCACAA TAACCAGTGA GTCCAGTCCT
    GGCAAGAGAG AGAAAGACAA AGAGAAAGAC AAAGAGAAAA GATTCAGCCT TTTTGGTAAA
    AAGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.