unc-70 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-70
Entrez Gene ID	179077
Full Name	Spectrin beta chain
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001028882.3	NP_001024053.2	Spectrin beta chain
NM_001307743.1	NP_001294672.1	Spectrin beta chain
NM_001028881.3	NP_001024052.1	Spectrin beta chain
NM_001269144.1	NP_001256073.1	Spectrin beta chain
NM_001269144.2	NP_001256073.1	Spectrin beta chain
NM_001028882.4	NP_001024053.2	Spectrin beta chain
NM_001028881.4	NP_001024052.1	Spectrin beta chain
NM_001307743.2	NP_001294672.1	Spectrin beta chain

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	SPTBN1	NP_001186354.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010895	XP_309796.2
Caenorhabditis elegans (roundworm)	unc-70	NP_001024053.1
Canis lupus familiaris (dog)	SPTBN1	XP_531827.2
Pan troglodytes (chimpanzee)	SPTBN1	XP_001154155.1
	SPTBN1	XP_001114804.1
Bos taurus (cattle)	SPTBN1	NP_001179205.1
Drosophila melanogaster (fruit fly)	beta-Spec	NP_001259660.1
Homo sapiens (human)	SPTBN1	NP_003119.2
Mus musculus (house mouse)	Sptbn1	NP_787030.2
Rattus norvegicus (Norway rat)	Sptbn1	NP_001013148.1
Danio rerio (zebrafish)	sptb	NP_571600.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Unc-70 is held under constitutive tension in living animals, which contributes to elevated pre-stress in touch receptor neurons.
Title: Mechanical control of the sense of touch by ?-spectrin.

beta-spectrin is required for the physical integrity of neuronal processes in the nematode Caenorhabditis elegans
Title: Axons break in animals lacking beta-spectrin.

The following unc-70 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-70 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa344840	NM_001028882.4 Latest version!	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa346226	NM_001307743.2 Latest version!	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa347847	NM_001028881.4 Latest version!	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OCa347849	NM_001269144.2 Latest version!	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa347849	NM_001269144.1	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa344840	NM_001028882.3	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa347847	NM_001028881.3	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCa346226	NM_001307743.1	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-70 ( NM_001028882.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024053.2 Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

Price & Availability↑

CloneID	OCa344840
Clone ID Related Accession (Same CDS sequence)	NM_001028882.3 , NM_001028882.4
Accession Version	NM_001028882.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Feb 27, 2013 this sequence version replaced NM_001028882.2. COMPLETENESS: incomplete on both ends.

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGACG CCGATATAAC CCACAGCAGT 
TTGGAGGGAC ACCGAAAGTC ATCACATGAG AAACGATTGG CCCACTCCGA GTCAAGCAGC 
TGGCGAAAGT CATTGGCGCG CGCGCCAAAG TTCGACAGCA AGGATCCAAA GGGAGCCAAA 
CAAGGAGAAG CATTCGAAGG AACGCTTATC AGAAAGCACA CTTACGAGAG TCTCGACCGG 
AAAGCGGCTA ACCGCTCATG GGAGAAACTT TACGCAGTGT TGCGGCAGAA TGAACTGTCA 
TTCTACAAAG ATCCGAAGCA TAGAGATGAA TCTGTGCATG GAGAGCCACC AATGGCACTG 
CCAGGATGCT CTGTCAACGT GGCATCAGAC TATCAAAAGA AGAAGAACGT ACTGTCGCTG 
AGGTTACCCA TCGGCGCCGA GTACCTATTC CAATGCGGTA GCGAAGAAGA CATGCAGCGA 
TGGCTCACCG AGTTGCAAGT CGCCACGGGG CAAGCCCAAC TGGAAGAGGC TAGTAGATCA 
CAGACGCTAC CAGCAGAAGG CAGTGCCACA AAGAAGAAGG GAGGATTTTT CTCTCGAGGC 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024053.2
CDS	1..6909
Translation	MATVDGNSGAYTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDDADITHSSLEGHRKSSHEKRLAHSESSSWRKSLARAPKFDSKDPKGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001028882.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028882.3

ORF Insert Sequence:

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGACG CCGATATAAC CCACAGCAGT 
TTGGAGGGAC ACCGAAAGTC ATCACATGAG AAACGATTGG CCCACTCCGA GTCAAGCAGC 
TGGCGAAAGT CATTGGCGCG CGCGCCAAAG TTCGACAGCA AGGATCCAAA GGGAGCCAAA 
CAAGGAGAAG CATTCGAAGG AACGCTTATC AGAAAGCACA CTTACGAGAG TCTCGACCGG 
AAAGCGGCTA ACCGCTCATG GGAGAAACTT TACGCAGTGT TGCGGCAGAA TGAACTGTCA 
TTCTACAAAG ATCCGAAGCA TAGAGATGAA TCTGTGCATG GAGAGCCACC AATGGCACTG 
CCAGGATGCT CTGTCAACGT GGCATCAGAC TATCAAAAGA AGAAGAACGT ACTGTCGCTG 
AGGTTACCCA TCGGCGCCGA GTACCTATTC CAATGCGGTA GCGAAGAAGA CATGCAGCGA 
TGGCTCACCG AGTTGCAAGT CGCCACGGGG CAAGCCCAAC TGGAAGAGGC TAGTAGATCA 
CAGACGCTAC CAGCAGAAGG CAGTGCCACA AAGAAGAAGG GAGGATTTTT CTCTCGAGGC 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001028882.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024053.2 Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

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CloneID	OCa344840
Clone ID Related Accession (Same CDS sequence)	NM_001028882.3 , NM_001028882.4
Accession Version	NM_001028882.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001028882.3.

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGACG CCGATATAAC CCACAGCAGT 
TTGGAGGGAC ACCGAAAGTC ATCACATGAG AAACGATTGG CCCACTCCGA GTCAAGCAGC 
TGGCGAAAGT CATTGGCGCG CGCGCCAAAG TTCGACAGCA AGGATCCAAA GGGAGCCAAA 
CAAGGAGAAG CATTCGAAGG AACGCTTATC AGAAAGCACA CTTACGAGAG TCTCGACCGG 
AAAGCGGCTA ACCGCTCATG GGAGAAACTT TACGCAGTGT TGCGGCAGAA TGAACTGTCA 
TTCTACAAAG ATCCGAAGCA TAGAGATGAA TCTGTGCATG GAGAGCCACC AATGGCACTG 
CCAGGATGCT CTGTCAACGT GGCATCAGAC TATCAAAAGA AGAAGAACGT ACTGTCGCTG 
AGGTTACCCA TCGGCGCCGA GTACCTATTC CAATGCGGTA GCGAAGAAGA CATGCAGCGA 
TGGCTCACCG AGTTGCAAGT CGCCACGGGG CAAGCCCAAC TGGAAGAGGC TAGTAGATCA 
CAGACGCTAC CAGCAGAAGG CAGTGCCACA AAGAAGAAGG GAGGATTTTT CTCTCGAGGC 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024053.2
CDS	49..6957
Translation	MATVDGNSGAYTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDDADITHSSLEGHRKSSHEKRLAHSESSSWRKSLARAPKFDSKDPKGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001028882.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028882.4

ORF Insert Sequence:

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGACG CCGATATAAC CCACAGCAGT 
TTGGAGGGAC ACCGAAAGTC ATCACATGAG AAACGATTGG CCCACTCCGA GTCAAGCAGC 
TGGCGAAAGT CATTGGCGCG CGCGCCAAAG TTCGACAGCA AGGATCCAAA GGGAGCCAAA 
CAAGGAGAAG CATTCGAAGG AACGCTTATC AGAAAGCACA CTTACGAGAG TCTCGACCGG 
AAAGCGGCTA ACCGCTCATG GGAGAAACTT TACGCAGTGT TGCGGCAGAA TGAACTGTCA 
TTCTACAAAG ATCCGAAGCA TAGAGATGAA TCTGTGCATG GAGAGCCACC AATGGCACTG 
CCAGGATGCT CTGTCAACGT GGCATCAGAC TATCAAAAGA AGAAGAACGT ACTGTCGCTG 
AGGTTACCCA TCGGCGCCGA GTACCTATTC CAATGCGGTA GCGAAGAAGA CATGCAGCGA 
TGGCTCACCG AGTTGCAAGT CGCCACGGGG CAAGCCCAAC TGGAAGAGGC TAGTAGATCA 
CAGACGCTAC CAGCAGAAGG CAGTGCCACA AAGAAGAAGG GAGGATTTTT CTCTCGAGGC 
AAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001307743.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294672.1 Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

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CloneID	OCa346226
Clone ID Related Accession (Same CDS sequence)	NM_001307743.1 , NM_001307743.2
Accession Version	NM_001307743.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGAGA AACGATTGGC CCACTCCGAG 
TCAAGCAGCT GGCGAAAGTC ATTGGCGCGC GCGCCAAAGT TCGACAGCAA GGATCCAAAG 
GGAGCCAAAC AAGGAGAAGC ATTCGAAGGA ACGCTTATCA GAAAGCACAC TTACGAGAGT 
CTCGACCGGA AAGCGGCTAA CCGCTCATGG GAGAAACTTT ACGCAGTGTT GCGGCAGAAT 
GAACTGTCAT TCTACAAAGA TCCGAAGCAT AGAGATGAAT CTGTGCATGG AGAGCCACCA 
ATGGCACTGC CAGGATGCTC TGTCAACGTG GCATCAGACT ATCAAAAGAA GAAGAACGTA 
CTGTCGCTGA GGTTACCCAT CGGCGCCGAG TACCTATTCC AATGCGGTAG CGAAGAAGAC 
ATGCAGCGAT GGCTCACCGA GTTGCAAGTC GCCACGGGGC AAGCCCAACT GGAAGAGGCT 
AGTAGATCAC AGACGCTACC AGCAGAAGGC AGTGCCACAA AGAAGAAGGG AGGATTTTTC 
TCTCGAGGCA AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294672.1
CDS	1..6858
Translation	MATVDGNSGAYTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDEKRLAHSESSSWRKSLARAPKFDSKDPKGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001307743.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307743.1

ORF Insert Sequence:

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGAGA AACGATTGGC CCACTCCGAG 
TCAAGCAGCT GGCGAAAGTC ATTGGCGCGC GCGCCAAAGT TCGACAGCAA GGATCCAAAG 
GGAGCCAAAC AAGGAGAAGC ATTCGAAGGA ACGCTTATCA GAAAGCACAC TTACGAGAGT 
CTCGACCGGA AAGCGGCTAA CCGCTCATGG GAGAAACTTT ACGCAGTGTT GCGGCAGAAT 
GAACTGTCAT TCTACAAAGA TCCGAAGCAT AGAGATGAAT CTGTGCATGG AGAGCCACCA 
ATGGCACTGC CAGGATGCTC TGTCAACGTG GCATCAGACT ATCAAAAGAA GAAGAACGTA 
CTGTCGCTGA GGTTACCCAT CGGCGCCGAG TACCTATTCC AATGCGGTAG CGAAGAAGAC 
ATGCAGCGAT GGCTCACCGA GTTGCAAGTC GCCACGGGGC AAGCCCAACT GGAAGAGGCT 
AGTAGATCAC AGACGCTACC AGCAGAAGGC AGTGCCACAA AGAAGAAGGG AGGATTTTTC 
TCTCGAGGCA AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001307743.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294672.1 Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

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CloneID	OCa346226
Clone ID Related Accession (Same CDS sequence)	NM_001307743.1 , NM_001307743.2
Accession Version	NM_001307743.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001307743.1.

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGAGA AACGATTGGC CCACTCCGAG 
TCAAGCAGCT GGCGAAAGTC ATTGGCGCGC GCGCCAAAGT TCGACAGCAA GGATCCAAAG 
GGAGCCAAAC AAGGAGAAGC ATTCGAAGGA ACGCTTATCA GAAAGCACAC TTACGAGAGT 
CTCGACCGGA AAGCGGCTAA CCGCTCATGG GAGAAACTTT ACGCAGTGTT GCGGCAGAAT 
GAACTGTCAT TCTACAAAGA TCCGAAGCAT AGAGATGAAT CTGTGCATGG AGAGCCACCA 
ATGGCACTGC CAGGATGCTC TGTCAACGTG GCATCAGACT ATCAAAAGAA GAAGAACGTA 
CTGTCGCTGA GGTTACCCAT CGGCGCCGAG TACCTATTCC AATGCGGTAG CGAAGAAGAC 
ATGCAGCGAT GGCTCACCGA GTTGCAAGTC GCCACGGGGC AAGCCCAACT GGAAGAGGCT 
AGTAGATCAC AGACGCTACC AGCAGAAGGC AGTGCCACAA AGAAGAAGGG AGGATTTTTC 
TCTCGAGGCA AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294672.1
CDS	47..6904
Translation	MATVDGNSGAYTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDEKRLAHSESSSWRKSLARAPKFDSKDPKGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001307743.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307743.2

ORF Insert Sequence:

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGAGA AACGATTGGC CCACTCCGAG 
TCAAGCAGCT GGCGAAAGTC ATTGGCGCGC GCGCCAAAGT TCGACAGCAA GGATCCAAAG 
GGAGCCAAAC AAGGAGAAGC ATTCGAAGGA ACGCTTATCA GAAAGCACAC TTACGAGAGT 
CTCGACCGGA AAGCGGCTAA CCGCTCATGG GAGAAACTTT ACGCAGTGTT GCGGCAGAAT 
GAACTGTCAT TCTACAAAGA TCCGAAGCAT AGAGATGAAT CTGTGCATGG AGAGCCACCA 
ATGGCACTGC CAGGATGCTC TGTCAACGTG GCATCAGACT ATCAAAAGAA GAAGAACGTA 
CTGTCGCTGA GGTTACCCAT CGGCGCCGAG TACCTATTCC AATGCGGTAG CGAAGAAGAC 
ATGCAGCGAT GGCTCACCGA GTTGCAAGTC GCCACGGGGC AAGCCCAACT GGAAGAGGCT 
AGTAGATCAC AGACGCTACC AGCAGAAGGC AGTGCCACAA AGAAGAAGGG AGGATTTTTC 
TCTCGAGGCA AGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001028881.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024052.1 Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

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CloneID	OCa347847
Clone ID Related Accession (Same CDS sequence)	NM_001028881.3 , NM_001028881.4
Accession Version	NM_001028881.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Jun 26, 2012 this sequence version replaced NM_001028881.2. COMPLETENESS: incomplete on both ends.

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGGAG CCAAACAAGG AGAAGCATTC 
GAAGGAACGC TTATCAGAAA GCACACTTAC GAGAGTCTCG ACCGGAAAGC GGCTAACCGC 
TCATGGGAGA AACTTTACGC AGTGTTGCGG CAGAATGAAC TGTCATTCTA CAAAGATCCG 
AAGCATAGAG ATGAATCTGT GCATGGAGAG CCACCAATGG CACTGCCAGG ATGCTCTGTC 
AACGTGGCAT CAGACTATCA AAAGAAGAAG AACGTACTGT CGCTGAGGTT ACCCATCGGC 
GCCGAGTACC TATTCCAATG CGGTAGCGAA GAAGACATGC AGCGATGGCT CACCGAGTTG 
CAAGTCGCCA CGGGGCAAGC CCAACTGGAA GAGGCTAGTA GATCACAGAC GCTACCAGCA 
GAAGGCAGTG CCACAAAGAA GAAGGGAGGA TTTTTCTCTC GAGGCAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024052.1
CDS	1..6774
Translation	MATVDGNSGAYTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001028881.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028881.3

ORF Insert Sequence:

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGGAG CCAAACAAGG AGAAGCATTC 
GAAGGAACGC TTATCAGAAA GCACACTTAC GAGAGTCTCG ACCGGAAAGC GGCTAACCGC 
TCATGGGAGA AACTTTACGC AGTGTTGCGG CAGAATGAAC TGTCATTCTA CAAAGATCCG 
AAGCATAGAG ATGAATCTGT GCATGGAGAG CCACCAATGG CACTGCCAGG ATGCTCTGTC 
AACGTGGCAT CAGACTATCA AAAGAAGAAG AACGTACTGT CGCTGAGGTT ACCCATCGGC 
GCCGAGTACC TATTCCAATG CGGTAGCGAA GAAGACATGC AGCGATGGCT CACCGAGTTG 
CAAGTCGCCA CGGGGCAAGC CCAACTGGAA GAGGCTAGTA GATCACAGAC GCTACCAGCA 
GAAGGCAGTG CCACAAAGAA GAAGGGAGGA TTTTTCTCTC GAGGCAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001028881.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001024052.1 Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

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CloneID	OCa347847
Clone ID Related Accession (Same CDS sequence)	NM_001028881.3 , NM_001028881.4
Accession Version	NM_001028881.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001028881.3.

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGGAG CCAAACAAGG AGAAGCATTC 
GAAGGAACGC TTATCAGAAA GCACACTTAC GAGAGTCTCG ACCGGAAAGC GGCTAACCGC 
TCATGGGAGA AACTTTACGC AGTGTTGCGG CAGAATGAAC TGTCATTCTA CAAAGATCCG 
AAGCATAGAG ATGAATCTGT GCATGGAGAG CCACCAATGG CACTGCCAGG ATGCTCTGTC 
AACGTGGCAT CAGACTATCA AAAGAAGAAG AACGTACTGT CGCTGAGGTT ACCCATCGGC 
GCCGAGTACC TATTCCAATG CGGTAGCGAA GAAGACATGC AGCGATGGCT CACCGAGTTG 
CAAGTCGCCA CGGGGCAAGC CCAACTGGAA GAGGCTAGTA GATCACAGAC GCTACCAGCA 
GAAGGCAGTG CCACAAAGAA GAAGGGAGGA TTTTTCTCTC GAGGCAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001024052.1
CDS	53..6826
Translation	MATVDGNSGAYTQDEDYDDNSSARLFERSRIKALADERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001028881.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001028881.4

ORF Insert Sequence:

ATGGCTACGG TCGACGGCAA TTCAGGCGCC TATACCCAAG ATGAGGACTA TGATGACAAC 
TCGTCCGCCC GCCTCTTCGA ACGTTCTCGT ATCAAGGCAC TAGCAGATGA ACGTGAACTC 
GTGCAAAAGA AAACATTTAC AAAATGGGTC AACTCACACT TGGTTCGAGT CAGCTGCAAA 
GTACAAGACC TCTACATGGA TATGCGAGAC GGAAAAATGC TCCTCCGACT CCTCGCAGTT 
CTTTCCGGTG AACGTCTGCC GAAACCGACG CCGGGAAAAA TGCGTATCCA CTGTCTTGAG 
AACGTTGAGA AAGGTTTGCA ATTCTTGCGC AATCAACACG TGCATCTCGA AAACTTGGGT 
TCACACGATA TCGTTGACGG AAATTCACGC CTCACGTTGG GTCTCATCTG GACGATCATC 
CTTCGATTCC AGATCCAAGA TATCACATTT GAAGACGCTG ACAATCACGA GACACGTTCA 
GCGAAGGAGG CCTTGTTACT GTGGTGCCAG ATGAAAACTG CTGGATATCC AAATGTCAAC 
GTCAAGAACT TCTCAACCAG TTGGAGAGAT GGTCTTGCAT TCAATGCACT TATTCATAAA 
CATAGACCTG ATCTCGTAGA CTACGATAAT TTACAAAAAT CGAATGCTCT TTACAATTTG 
CAATCTGCTT TTGATACCGC AGAAAATCAA TTGGGATTGG CCAAGTTCCT CGATGCCGAA 
GATGTGAACG TTGACCAACC TGATGAGAAA TCCATTATCA CTTATGTAGT TACATATTAT 
CATTACTTCA ACAAACTGAA ACAAGATAAC ATCCAAGGAA AACGTATCGG AAAAGTGATT 
AATGAGTTGA TGGAGAACGA CAAAATGATC AATCGATACG AAACTCTTTC ATCTGATTTG 
CTCGAATGGA TCAATGCCAA GATCCAACTT CTCAACGAAC GTCATTTTGA GAATAATTTG 
GAAGGTGTTC AAAGACAGCT CACCGAGTTC AACGACTACC GTACTCAGGA GAAACCACCA 
AAGTTCGATG AAAAGGGAGA GCTCGAAGTA CTTCTGTTCA CTCTTCAATC AGCTATGCGT 
GCCAACAATC AGAGACCTTT CGTGCCGAGA GAAGGAAAAC TGATTGCTGA TATCAACCGA 
GCATGGCAAT CCCTTGAGAA GGCAGAACAC GAACGAGAAC TTGTCCTGAA AGAGGAACTC 
ATTCGTCAAG AGAAACTCGA ACAACTCGCA GCCCGATTCA ACAGAAAGGC CGAGATGCGA 
GAGACTTGGC TCACAGAAAA CCAACGGCTT GTCAGTCAGG ACAATTTCGG AAACGACTTG 
TCTTCGGTGG AAGCTGCCAC CAAAAAGCAC GAAGCCATCG AGACGGACAT CTTTGCTTAC 
GAGGAGCGTG TTCAAGCCGT CGTCGCTGTT GCTGGAGAGC TTGAAGCCGA GAACTACCAT 
GATCAAGCCA AAATCAATGA GAGAAAGGAG AATGTTCTTC AACTCTGGAA CTATCTTTTC 
CAACTTCTTT TGGCTCGCAG AGTTCGATTG GAGTTGTCAA TGGCCATTCA AAAGATCTTC 
CACGATATGC TTCTTACTCT CGATCTCATG GATGATATTA AGAGCAGACT TTTAAGCGAG 
GATCTTGGAG CCCATCTTAT GGACGTAGAA GATTTGCTTC AAAAGCACGC TCTTCTTGAG 
TCTGATATTA ATATTATTGG AGAACGAGTT AACAATTCTA TTGCTCAAGC TCAACGCTTC 
CGAAATCCAG ATGGACCAGA TGGAAGTGGA TACAAGCCAG TTGAACCAGG AACCATTGAT 
GAGAGAAGTG ATGTTCTACA AAAACGTTAC AAGGAGCTTC TTGATTTGGC TGCTGAACGT 
AAGCGTCGTC TCGAAGACAA CAAGAGACTT TGCCAGTTCT GGTGGGATGT TGCTGAGCTT 
GAACATGGTA TTAAGGAACA AGAGCAAGTT CTTTCGTCCA CTGATACTGG CCGTGATATT 
GTCACTGTTT CGCATTTGTT GGCCAAACAT AAGAACGCTG AGAACAACCT TCGCGACTTG 
GAGAAGTACC TCGATCGTCT TGATGTTTCT GGAAAAGAAT TGCAAGATGA AAGCATCCCA 
GGAAGTGACA ACATTCCTCC AAGGCTTGCC GAGATTCGTG ATTACATCAA CAAGCTCAAG 
GAACTCTCTG CTTCTCGCAA AGAAAGACTT GCCGGTGGAG TCGAATACTA CCAATTCTTC 
ACCGACGCCG ACGATGTCGA TCGCTACTTG TATGATACTC TCCGCGTCAT GTCATCCGAA 
GACGTTGGAA AAGACGAGGG AACTGTTCAA CTTCTCCTCA AGAAGCACGA CGATGTCCAT 
GACGAGCTCC AGAACTTTGA CCAACACATC AAGGTTCTTC ACGCCAAGGC CGAGTCTCTT 
CCACAGGAAG CCAGAGAACA TCCAGATATC AGACAACGAT TGGACACAAC TCTCAAGCAG 
AAGGCCGAAC TCGAAAACCT TTCTCAACTT CGCAAGCAGA GACTTATCGA TGCTCTCTCC 
CTGTACAAGT TGTACTCGGA TGCCGATTCA GTCGAGTCTT GGATCGATGA GAAGGGAAAA 
CTTTTGGCAA CATTGGTCCC TGGACGCGAT ATCGAAGAGG TTGAGATCAT GAAGCATCGT 
TTCGATACTC TTGAGCAAGA TATGAAGAAT CAAGAAGCTA AGGTTACTAA CGTCAATGAC 
TTGGCTCGTC AATTGCTTAA TGTGGAACAT CCAAACTCCG ACGACATTCT TCACCGTCAA 
AACAAGCTCA ACGCTCGCTG GGCTCAACTT CGCGACATGG TCGATCAAAA GAGAAACGAG 
CTCGAGAGAG CTCACCGCCT CGAAACTTTC CGCATCGATT GCCAAGAAAC AGTTACTTGG 
ATCGAGGACA AGACTCGTGT ACTCGAGGAT TCGGACGCGT TGACCAACGA TCTTTCCGGT 
GTCATGAAAC TTCAAAGAAG ATTGTCCATG ATGGAACGCG ATTTGGGAGC GATTCAAGCC 
AAGCTCGACT CGCTTCACAA AGAAGCCGAT GACATCGAAA GAGAACGACC ACAAGAAGCT 
CAAGCCATTC GTGAAGATAT CAAGAGAATC CACCAAGTCT GGGATATCCT TAACAAGAAA 
GTTCGTGAGC ACGAAGCTAA ACTCGATGAA GCTGGAGATC TTCAACGCTT CCTCCGTGAT 
TTGGATCACT TCCAAGCCTG GCTTACTGCC ACCCAGAGAC AAGTTGCCTC AGAGGAAGAG 
CCACAATCCT TGGCTGAAGC TGAGCAACTT CTCAATCAAC ACGCTGCCAT CCGTGAAGAG 
ATTGACGGAT ACGCTGAGGA TTACAAGAAG ATGCGTGCAA TGGGAGATCG TGTCACTCAA 
GATCAAACCG ATCCACAATA CATGTTCCTT CGTCAACGTC TTGCTGGACT TCAAGAAGGA 
TGGGAAGAGT TGCAGCGAAT GTGGGACAAC AGACAACATT TGCTTTCTCA AGGACTCAAC 
CTTCAAATGT TCCTTCGCGA TGCCAAACAA GCAGAGGTTA TGCTCTCTCA ACAAGAAAAC 
TATCTCGCCA AGGACGATAT CCCACAATCT TTGGAGCAAG CCGAGAACCA ACTCAAGAGA 
CACCAAGACT TCATCACCAC TATGGATGCC AACGACGAAA AGATTCGCGC CGTCGGAATG 
TTCGGTGATC AGCTCTGCCA AGATGGTCAC TATGCCGCTG ACAAGATCCA TAAGAAGGCT 
AGAAACATTG ATGAACGTCG TGGAGCCAAC CGCGAGAAGG CTCAAGAAGT ACTCAAGAAG 
CTGAAGGACG CCTTGTCTCT CCAACAATTC TTGTCCGACT GCGACGAGCT GAGAGAATGG 
ATCGAGGAGA AGATGATCCG TGCTCAAGAT GAGACTTACA GAGACGCCAA GACTATCACC 
TCCAAATTCG TCCGACATCA AGCATTCCAA TCCGAACTTG CTGCTAACAA GGAACGTCTT 
GACCAACTTA AACATGCTGC CATCAACCTT GGTGACGACA AGCCAGAGTA CCACGGAACC 
ATTGATCCAC AGATCGAGGA ACTCGCTACT CAATGGGATG AGTTGGAAAA GACTACAGAG 
GAGAAGGGAC AGAAGTTGTT TGATGCTAAC CGCCAACAAC TTTACGTACA AAGTATTGCT 
GACATGAAGG AATGGGCAAC CCAACTTGAG AACGAGATGA CTCGTGAAGA CCAACCAGGA 
GATCTTACCA CTGTCAATGT GGCCATGCAA AAACAACATT TGATCGAAAC TGAAATGATC 
AAGAAGGCTC AACACATTGA TCAACTCATG GAGATGGAGC CACAGCTCGA GGAACTTCAC 
CCAGACGAGC TTGAAAACAT TAAGGCTCAT CGTTTGGCAG TTCAAGAACA ACTTCAAAGA 
CTCCAGGCTC CATTGGACGA CCGACGTAAG GCATTGGAAC GCAAGAAGGC CGCCTTCCAA 
TTTGGTCGCG ATGTTGATGA CGAGAAATTG TGGATTTCTG AGAGACTTGT TCTCGCTAAA 
GCTCAAAATC TTGGAGAAAG CCTTCCAGAT TGTCATCGTC TCCAGAAGAA CCTACAACTT 
CTTTCCAATG AAATCGACAA CCACGAACCA TGGATCAACC AGATCTGTAA TAATGGTCAA 
GAGTTGATTG ACGAAGGACA TGCTAACGGA CCAGCATTCG AGAAGAAGAT TCAAGAACTG 
CGCAGTGCTT GGCAAGAGTT GAAGGAAGCC GTCAAAGACA GAAAGGGAGA TCTTGGAGAA 
AGCGAGAAGG CTCATCAATT CTTGTATGAT TGCGGAGAGG CTGAAGCCTG GATGAGCGAA 
CAAGAATTGT ACATGATGCA AGACGAAAGA GGAAAAGACG AGTTCTCAAC CAAGAACCAA 
ATCAAGAAAC ACGAACGCCT TCAATCCGAT ATTGACAAAT TTGCTGATAC GATCCGTGCT 
CTTGCCACCA AGGCTCACAA GTTCGTCGAA GAGAAGTCAC CACTCACCGA ACAGATCCAA 
GTTCGTCAAG CTCAAATCGA GAAGCTCTAC GCAGGTCTTC AAGATTTGTC GAAGGAGCGC 
AGAAAGAGAC TCGAAGAGAC CTTGGAACTT TACGCTCTTC ATCGTGAAAT CGACGATCTT 
CTTCAATGGA TCGCCGACAA GGAAGTTGTT GCAGGAAGTC AAGAGAACGG ACAAGACTAC 
GAACACGTCC AAATGCTTCA AGAGAGATTC CAACAGTTTG CTCGTGACAC CGAAAACATC 
GGATCCGAAC GTGTTGCCAA TGCTAATGAT GGATGTGATA CTCTCATTGG TCACGGACAC 
ACTGACGCTC CGACTATAGC ATTGTGGAAG GATTCGTTGA ATGAGGCCTG GGAGAATCTT 
CTGGAGTTGA TGGACACTCG TGCCCAAATT CTCGAGGCTT CTAGACTTCT TCACAAGTTC 
TACCACGACT GCCGTGATTG CTTGTCAAGA ATCATGGAAA AGACTCATGC TATGCCTGAT 
GATCTTGGAC GTGACTCAAG CAGTGTTGGT GCACTCTCCA GAAAACATCA GAACTATCTC 
AAGGATATTG CTGCAATTGG AGAACAAGTT GCTCAAATCG AAAGAGATGC CGCAGAGCTT 
CGAGATGGTT ACGCTGGAGA CAAGGCTCTT GATATCGGAT CTCGCGAAAG CGAGGTTGTC 
AAGGCATGGA GGCATCTCCG CGGACTCTGT GACGCCCGTA CATCTCGTCT CATGGATACT 
TCTGATCTTT TCAAGTTCAT GAATATGGTC CGTGATTTGT TGTTGTGGAT GGATGAGGTG 
AAGAGAGAAA TGAACTCACA AGAACGTCCG AAGGATGTGA GCGGAGTCGA GTTGCTCATG 
AACAATCATC AATCGCTCAA GGCTGAAATC GATGCTCGCG AGGAGAACTT CAACGCGTGC 
ATCTCATTGG GACGAGATCT GCTCAACAGA AAACACTACG CATCAAGTGA AATCGAGAAG 
AAGCTGATCA AGTTGACAAC CGAAAGAGCC GAAATGATGC GCAGATGGGA AGATCGTTGG 
GAATATCTTC AGCTCATTCT GGAAGTGTAC CAATTCGCCC GCGACGCTGC TGTCGCCGAA 
TCTTGGCTCT TCGCCCAAGA ACCATATCTT ATTTCGAAGG AATACGGTAG AAACCTCGAA 
GAAACCATCA AACTCATCAA GAAACACGAG GCGTTCGAGA AATCGGCATT CGCACAAGAA 
GAACGTTTCT TGGCGCTCGA GAAATTGACA ACGTTCGAAT TGAAAGAAAC TCAACACCGC 
GAGGAGGAGA CCGCCAAGCG ACGTGGTCCA GCTCATATTG GAAGTCCTTC TAGGTCAACT 
CCAGCCGCGG AAACATCTTT CGGCGCCCAG GATGATGGAG CCAAACAAGG AGAAGCATTC 
GAAGGAACGC TTATCAGAAA GCACACTTAC GAGAGTCTCG ACCGGAAAGC GGCTAACCGC 
TCATGGGAGA AACTTTACGC AGTGTTGCGG CAGAATGAAC TGTCATTCTA CAAAGATCCG 
AAGCATAGAG ATGAATCTGT GCATGGAGAG CCACCAATGG CACTGCCAGG ATGCTCTGTC 
AACGTGGCAT CAGACTATCA AAAGAAGAAG AACGTACTGT CGCTGAGGTT ACCCATCGGC 
GCCGAGTACC TATTCCAATG CGGTAGCGAA GAAGACATGC AGCGATGGCT CACCGAGTTG 
CAAGTCGCCA CGGGGCAAGC CCAACTGGAA GAGGCTAGTA GATCACAGAC GCTACCAGCA 
GAAGGCAGTG CCACAAAGAA GAAGGGAGGA TTTTTCTCTC GAGGCAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001269144.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256073.1 Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

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CloneID	OCa347849
Clone ID Related Accession (Same CDS sequence)	NM_001269144.1 , NM_001269144.2
Accession Version	NM_001269144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). COMPLETENESS: incomplete on both ends.

ATGTCGTCGG TGGATGACGA GCATCATCCT TCAATTGAAG TTGATATTCC TGACGTTTAT 
GAAAGTGCAT CTGACTGTGA AAAAACTGAA GACTGCACCT ATCTAGGATC TATTCTCAAG 
GCGAAAAAAT CATTGCGAAA AACCAAGAAA GTCGCTAGTC CTGGTATCTA TCCGGGCTCG 
GCGGGTGCTC TTTCATCTCT TTCAAATGGC CTAGCCGATG AGAATAGATC GCCAACTAGT 
GCTGAAGTTT CTGATTCAGT ATGTGGACAA AAATCTATAA ATTCAGTTGA CTTGAGGTTC 
AGAGGATTGA GAGATGAACG TGAACTCGTG CAAAAGAAAA CATTTACAAA ATGGGTCAAC 
TCACACTTGG TTCGAGTCAG CTGCAAAGTA CAAGACCTCT ACATGGATAT GCGAGACGGA 
AAAATGCTCC TCCGACTCCT CGCAGTTCTT TCCGGTGAAC GTCTGCCGAA ACCGACGCCG 
GGAAAAATGC GTATCCACTG TCTTGAGAAC GTTGAGAAAG GTTTGCAATT CTTGCGCAAT 
CAACACGTGC ATCTCGAAAA CTTGGGTTCA CACGATATCG TTGACGGAAA TTCACGCCTC 
ACGTTGGGTC TCATCTGGAC GATCATCCTT CGATTCCAGA TCCAAGATAT CACATTTGAA 
GACGCTGACA ATCACGAGAC ACGTTCAGCG AAGGAGGCCT TGTTACTGTG GTGCCAGATG 
AAAACTGCTG GATATCCAAA TGTCAACGTC AAGAACTTCT CAACCAGTTG GAGAGATGGT 
CTTGCATTCA ATGCACTTAT TCATAAACAT AGACCTGATC TCGTAGACTA CGATAATTTA 
CAAAAATCGA ATGCTCTTTA CAATTTGCAA TCTGCTTTTG ATACCGCAGA AAATCAATTG 
GGATTGGCCA AGTTCCTCGA TGCCGAAGAT GTGAACGTTG ACCAACCTGA TGAGAAATCC 
ATTATCACTT ATGTAGTTAC ATATTATCAT TACTTCAACA AACTGAAACA AGATAACATC 
CAAGGAAAAC GTATCGGAAA AGTGATTAAT GAGTTGATGG AGAACGACAA AATGATCAAT 
CGATACGAAA CTCTTTCATC TGATTTGCTC GAATGGATCA ATGCCAAGAT CCAACTTCTC 
AACGAACGTC ATTTTGAGAA TAATTTGGAA GGTGTTCAAA GACAGCTCAC CGAGTTCAAC 
GACTACCGTA CTCAGGAGAA ACCACCAAAG TTCGATGAAA AGGGAGAGCT CGAAGTACTT 
CTGTTCACTC TTCAATCAGC TATGCGTGCC AACAATCAGA GACCTTTCGT GCCGAGAGAA 
GGAAAACTGA TTGCTGATAT CAACCGAGCA TGGCAATCCC TTGAGAAGGC AGAACACGAA 
CGAGAACTTG TCCTGAAAGA GGAACTCATT CGTCAAGAGA AACTCGAACA ACTCGCAGCC 
CGATTCAACA GAAAGGCCGA GATGCGAGAG ACTTGGCTCA CAGAAAACCA ACGGCTTGTC 
AGTCAGGACA ATTTCGGAAA CGACTTGTCT TCGGTGGAAG CTGCCACCAA AAAGCACGAA 
GCCATCGAGA CGGACATCTT TGCTTACGAG GAGCGTGTTC AAGCCGTCGT CGCTGTTGCT 
GGAGAGCTTG AAGCCGAGAA CTACCATGAT CAAGCCAAAA TCAATGAGAG AAAGGAGAAT 
GTTCTTCAAC TCTGGAACTA TCTTTTCCAA CTTCTTTTGG CTCGCAGAGT TCGATTGGAG 
TTGTCAATGG CCATTCAAAA GATCTTCCAC GATATGCTTC TTACTCTCGA TCTCATGGAT 
GATATTAAGA GCAGACTTTT AAGCGAGGAT CTTGGAGCCC ATCTTATGGA CGTAGAAGAT 
TTGCTTCAAA AGCACGCTCT TCTTGAGTCT GATATTAATA TTATTGGAGA ACGAGTTAAC 
AATTCTATTG CTCAAGCTCA ACGCTTCCGA AATCCAGATG GACCAGATGG AAGTGGATAC 
AAGCCAGTTG AACCAGGAAC CATTGATGAG AGAAGTGATG TTCTACAAAA ACGTTACAAG 
GAGCTTCTTG ATTTGGCTGC TGAACGTAAG CGTCGTCTCG AAGACAACAA GAGACTTTGC 
CAGTTCTGGT GGGATGTTGC TGAGCTTGAA CATGGTATTA AGGAACAAGA GCAAGTTCTT 
TCGTCCACTG ATACTGGCCG TGATATTGTC ACTGTTTCGC ATTTGTTGGC CAAACATAAG 
AACGCTGAGA ACAACCTTCG CGACTTGGAG AAGTACCTCG ATCGTCTTGA TGTTTCTGGA 
AAAGAATTGC AAGATGAAAG CATCCCAGGA AGTGACAACA TTCCTCCAAG GCTTGCCGAG 
ATTCGTGATT ACATCAACAA GCTCAAGGAA CTCTCTGCTT CTCGCAAAGA AAGACTTGCC 
GGTGGAGTCG AATACTACCA ATTCTTCACC GACGCCGACG ATGTCGATCG CTACTTGTAT 
GATACTCTCC GCGTCATGTC ATCCGAAGAC GTTGGAAAAG ACGAGGGAAC TGTTCAACTT 
CTCCTCAAGA AGCACGACGA TGTCCATGAC GAGCTCCAGA ACTTTGACCA ACACATCAAG 
GTTCTTCACG CCAAGGCCGA GTCTCTTCCA CAGGAAGCCA GAGAACATCC AGATATCAGA 
CAACGATTGG ACACAACTCT CAAGCAGAAG GCCGAACTCG AAAACCTTTC TCAACTTCGC 
AAGCAGAGAC TTATCGATGC TCTCTCCCTG TACAAGTTGT ACTCGGATGC CGATTCAGTC 
GAGTCTTGGA TCGATGAGAA GGGAAAACTT TTGGCAACAT TGGTCCCTGG ACGCGATATC 
GAAGAGGTTG AGATCATGAA GCATCGTTTC GATACTCTTG AGCAAGATAT GAAGAATCAA 
GAAGCTAAGG TTACTAACGT CAATGACTTG GCTCGTCAAT TGCTTAATGT GGAACATCCA 
AACTCCGACG ACATTCTTCA CCGTCAAAAC AAGCTCAACG CTCGCTGGGC TCAACTTCGC 
GACATGGTCG ATCAAAAGAG AAACGAGCTC GAGAGAGCTC ACCGCCTCGA AACTTTCCGC 
ATCGATTGCC AAGAAACAGT TACTTGGATC GAGGACAAGA CTCGTGTACT CGAGGATTCG 
GACGCGTTGA CCAACGATCT TTCCGGTGTC ATGAAACTTC AAAGAAGATT GTCCATGATG 
GAACGCGATT TGGGAGCGAT TCAAGCCAAG CTCGACTCGC TTCACAAAGA AGCCGATGAC 
ATCGAAAGAG AACGACCACA AGAAGCTCAA GCCATTCGTG AAGATATCAA GAGAATCCAC 
CAAGTCTGGG ATATCCTTAA CAAGAAAGTT CGTGAGCACG AAGCTAAACT CGATGAAGCT 
GGAGATCTTC AACGCTTCCT CCGTGATTTG GATCACTTCC AAGCCTGGCT TACTGCCACC 
CAGAGACAAG TTGCCTCAGA GGAAGAGCCA CAATCCTTGG CTGAAGCTGA GCAACTTCTC 
AATCAACACG CTGCCATCCG TGAAGAGATT GACGGATACG CTGAGGATTA CAAGAAGATG 
CGTGCAATGG GAGATCGTGT CACTCAAGAT CAAACCGATC CACAATACAT GTTCCTTCGT 
CAACGTCTTG CTGGACTTCA AGAAGGATGG GAAGAGTTGC AGCGAATGTG GGACAACAGA 
CAACATTTGC TTTCTCAAGG ACTCAACCTT CAAATGTTCC TTCGCGATGC CAAACAAGCA 
GAGGTTATGC TCTCTCAACA AGAAAACTAT CTCGCCAAGG ACGATATCCC ACAATCTTTG 
GAGCAAGCCG AGAACCAACT CAAGAGACAC CAAGACTTCA TCACCACTAT GGATGCCAAC 
GACGAAAAGA TTCGCGCCGT CGGAATGTTC GGTGATCAGC TCTGCCAAGA TGGTCACTAT 
GCCGCTGACA AGATCCATAA GAAGGCTAGA AACATTGATG AACGTCGTGG AGCCAACCGC 
GAGAAGGCTC AAGAAGTACT CAAGAAGCTG AAGGACGCCT TGTCTCTCCA ACAATTCTTG 
TCCGACTGCG ACGAGCTGAG AGAATGGATC GAGGAGAAGA TGATCCGTGC TCAAGATGAG 
ACTTACAGAG ACGCCAAGAC TATCACCTCC AAATTCGTCC GACATCAAGC ATTCCAATCC 
GAACTTGCTG CTAACAAGGA ACGTCTTGAC CAACTTAAAC ATGCTGCCAT CAACCTTGGT 
GACGACAAGC CAGAGTACCA CGGAACCATT GATCCACAGA TCGAGGAACT CGCTACTCAA 
TGGGATGAGT TGGAAAAGAC TACAGAGGAG AAGGGACAGA AGTTGTTTGA TGCTAACCGC 
CAACAACTTT ACGTACAAAG TATTGCTGAC ATGAAGGAAT GGGCAACCCA ACTTGAGAAC 
GAGATGACTC GTGAAGACCA ACCAGGAGAT CTTACCACTG TCAATGTGGC CATGCAAAAA 
CAACATTTGA TCGAAACTGA AATGATCAAG AAGGCTCAAC ACATTGATCA ACTCATGGAG 
ATGGAGCCAC AGCTCGAGGA ACTTCACCCA GACGAGCTTG AAAACATTAA GGCTCATCGT 
TTGGCAGTTC AAGAACAACT TCAAAGACTC CAGGCTCCAT TGGACGACCG ACGTAAGGCA 
TTGGAACGCA AGAAGGCCGC CTTCCAATTT GGTCGCGATG TTGATGACGA GAAATTGTGG 
ATTTCTGAGA GACTTGTTCT CGCTAAAGCT CAAAATCTTG GAGAAAGCCT TCCAGATTGT 
CATCGTCTCC AGAAGAACCT ACAACTTCTT TCCAATGAAA TCGACAACCA CGAACCATGG 
ATCAACCAGA TCTGTAATAA TGGTCAAGAG TTGATTGACG AAGGACATGC TAACGGACCA 
GCATTCGAGA AGAAGATTCA AGAACTGCGC AGTGCTTGGC AAGAGTTGAA GGAAGCCGTC 
AAAGACAGAA AGGGAGATCT TGGAGAAAGC GAGAAGGCTC ATCAATTCTT GTATGATTGC 
GGAGAGGCTG AAGCCTGGAT GAGCGAACAA GAATTGTACA TGATGCAAGA CGAAAGAGGA 
AAAGACGAGT TCTCAACCAA GAACCAAATC AAGAAACACG AACGCCTTCA ATCCGATATT 
GACAAATTTG CTGATACGAT CCGTGCTCTT GCCACCAAGG CTCACAAGTT CGTCGAAGAG 
AAGTCACCAC TCACCGAACA GATCCAAGTT CGTCAAGCTC AAATCGAGAA GCTCTACGCA 
GGTCTTCAAG ATTTGTCGAA GGAGCGCAGA AAGAGACTCG AAGAGACCTT GGAACTTTAC 
GCTCTTCATC GTGAAATCGA CGATCTTCTT CAATGGATCG CCGACAAGGA AGTTGTTGCA 
GGAAGTCAAG AGAACGGACA AGACTACGAA CACGTCCAAA TGCTTCAAGA GAGATTCCAA 
CAGTTTGCTC GTGACACCGA AAACATCGGA TCCGAACGTG TTGCCAATGC TAATGATGGA 
TGTGATACTC TCATTGGTCA CGGACACACT GACGCTCCGA CTATAGCATT GTGGAAGGAT 
TCGTTGAATG AGGCCTGGGA GAATCTTCTG GAGTTGATGG ACACTCGTGC CCAAATTCTC 
GAGGCTTCTA GACTTCTTCA CAAGTTCTAC CACGACTGCC GTGATTGCTT GTCAAGAATC 
ATGGAAAAGA CTCATGCTAT GCCTGATGAT CTTGGACGTG ACTCAAGCAG TGTTGGTGCA 
CTCTCCAGAA AACATCAGAA CTATCTCAAG GATATTGCTG CAATTGGAGA ACAAGTTGCT 
CAAATCGAAA GAGATGCCGC AGAGCTTCGA GATGGTTACG CTGGAGACAA GGCTCTTGAT 
ATCGGATCTC GCGAAAGCGA GGTTGTCAAG GCATGGAGGC ATCTCCGCGG ACTCTGTGAC 
GCCCGTACAT CTCGTCTCAT GGATACTTCT GATCTTTTCA AGTTCATGAA TATGGTCCGT 
GATTTGTTGT TGTGGATGGA TGAGGTGAAG AGAGAAATGA ACTCACAAGA ACGTCCGAAG 
GATGTGAGCG GAGTCGAGTT GCTCATGAAC AATCATCAAT CGCTCAAGGC TGAAATCGAT 
GCTCGCGAGG AGAACTTCAA CGCGTGCATC TCATTGGGAC GAGATCTGCT CAACAGAAAA 
CACTACGCAT CAAGTGAAAT CGAGAAGAAG CTGATCAAGT TGACAACCGA AAGAGCCGAA 
ATGATGCGCA GATGGGAAGA TCGTTGGGAA TATCTTCAGC TCATTCTGGA AGTGTACCAA 
TTCGCCCGCG ACGCTGCTGT CGCCGAATCT TGGCTCTTCG CCCAAGAACC ATATCTTATT 
TCGAAGGAAT ACGGTAGAAA CCTCGAAGAA ACCATCAAAC TCATCAAGAA ACACGAGGCG 
TTCGAGAAAT CGGCATTCGC ACAAGAAGAA CGTTTCTTGG CGCTCGAGAA ATTGACAACG 
TTCGAATTGA AAGAAACTCA ACACCGCGAG GAGGAGACCG CCAAGCGACG TGGTCCAGCT 
CATATTGGAA GTCCTTCTAG GTCAACTCCA GCCGCGGAAA CATCTTTCGG CGCCCAGGAT 
GATGGAGCCA AACAAGGAGA AGCATTCGAA GGAACGCTTA TCAGAAAGCA CACTTACGAG 
AGTCTCGACC GGAAAGCGGC TAACCGCTCA TGGGAGAAAC TTTACGCAGT GTTGCGGCAG 
AATGAACTGT CATTCTACAA AGATCCGAAG CATAGAGATG AATCTGTGCA TGGAGAGCCA 
CCAATGGCAC TGCCAGGATG CTCTGTCAAC GTGGCATCAG ACTATCAAAA GAAGAAGAAC 
GTACTGTCGC TGAGGTTACC CATCGGCGCC GAGTACCTAT TCCAATGCGG TAGCGAAGAA 
GACATGCAGC GATGGCTCAC CGAGTTGCAA GTCGCCACGG GGCAAGCCCA ACTGGAAGAG 
GCTAGTAGAT CACAGACGCT ACCAGCAGAA GGCAGTGCCA CAAAGAAGAA GGGAGGATTT 
TTCTCTCGAG GCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256073.1
CDS	1..6981
Translation	MSSVDDEHHPSIEVDIPDVYESASDCEKTEDCTYLGSILKAKKSLRKTKKVASPGIYPGSAGALSSLSNGLADENRSPTSAEVSDSVCGQKSINSVDLRFRGLRDERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001269144.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269144.1

ORF Insert Sequence:

ATGTCGTCGG TGGATGACGA GCATCATCCT TCAATTGAAG TTGATATTCC TGACGTTTAT 
GAAAGTGCAT CTGACTGTGA AAAAACTGAA GACTGCACCT ATCTAGGATC TATTCTCAAG 
GCGAAAAAAT CATTGCGAAA AACCAAGAAA GTCGCTAGTC CTGGTATCTA TCCGGGCTCG 
GCGGGTGCTC TTTCATCTCT TTCAAATGGC CTAGCCGATG AGAATAGATC GCCAACTAGT 
GCTGAAGTTT CTGATTCAGT ATGTGGACAA AAATCTATAA ATTCAGTTGA CTTGAGGTTC 
AGAGGATTGA GAGATGAACG TGAACTCGTG CAAAAGAAAA CATTTACAAA ATGGGTCAAC 
TCACACTTGG TTCGAGTCAG CTGCAAAGTA CAAGACCTCT ACATGGATAT GCGAGACGGA 
AAAATGCTCC TCCGACTCCT CGCAGTTCTT TCCGGTGAAC GTCTGCCGAA ACCGACGCCG 
GGAAAAATGC GTATCCACTG TCTTGAGAAC GTTGAGAAAG GTTTGCAATT CTTGCGCAAT 
CAACACGTGC ATCTCGAAAA CTTGGGTTCA CACGATATCG TTGACGGAAA TTCACGCCTC 
ACGTTGGGTC TCATCTGGAC GATCATCCTT CGATTCCAGA TCCAAGATAT CACATTTGAA 
GACGCTGACA ATCACGAGAC ACGTTCAGCG AAGGAGGCCT TGTTACTGTG GTGCCAGATG 
AAAACTGCTG GATATCCAAA TGTCAACGTC AAGAACTTCT CAACCAGTTG GAGAGATGGT 
CTTGCATTCA ATGCACTTAT TCATAAACAT AGACCTGATC TCGTAGACTA CGATAATTTA 
CAAAAATCGA ATGCTCTTTA CAATTTGCAA TCTGCTTTTG ATACCGCAGA AAATCAATTG 
GGATTGGCCA AGTTCCTCGA TGCCGAAGAT GTGAACGTTG ACCAACCTGA TGAGAAATCC 
ATTATCACTT ATGTAGTTAC ATATTATCAT TACTTCAACA AACTGAAACA AGATAACATC 
CAAGGAAAAC GTATCGGAAA AGTGATTAAT GAGTTGATGG AGAACGACAA AATGATCAAT 
CGATACGAAA CTCTTTCATC TGATTTGCTC GAATGGATCA ATGCCAAGAT CCAACTTCTC 
AACGAACGTC ATTTTGAGAA TAATTTGGAA GGTGTTCAAA GACAGCTCAC CGAGTTCAAC 
GACTACCGTA CTCAGGAGAA ACCACCAAAG TTCGATGAAA AGGGAGAGCT CGAAGTACTT 
CTGTTCACTC TTCAATCAGC TATGCGTGCC AACAATCAGA GACCTTTCGT GCCGAGAGAA 
GGAAAACTGA TTGCTGATAT CAACCGAGCA TGGCAATCCC TTGAGAAGGC AGAACACGAA 
CGAGAACTTG TCCTGAAAGA GGAACTCATT CGTCAAGAGA AACTCGAACA ACTCGCAGCC 
CGATTCAACA GAAAGGCCGA GATGCGAGAG ACTTGGCTCA CAGAAAACCA ACGGCTTGTC 
AGTCAGGACA ATTTCGGAAA CGACTTGTCT TCGGTGGAAG CTGCCACCAA AAAGCACGAA 
GCCATCGAGA CGGACATCTT TGCTTACGAG GAGCGTGTTC AAGCCGTCGT CGCTGTTGCT 
GGAGAGCTTG AAGCCGAGAA CTACCATGAT CAAGCCAAAA TCAATGAGAG AAAGGAGAAT 
GTTCTTCAAC TCTGGAACTA TCTTTTCCAA CTTCTTTTGG CTCGCAGAGT TCGATTGGAG 
TTGTCAATGG CCATTCAAAA GATCTTCCAC GATATGCTTC TTACTCTCGA TCTCATGGAT 
GATATTAAGA GCAGACTTTT AAGCGAGGAT CTTGGAGCCC ATCTTATGGA CGTAGAAGAT 
TTGCTTCAAA AGCACGCTCT TCTTGAGTCT GATATTAATA TTATTGGAGA ACGAGTTAAC 
AATTCTATTG CTCAAGCTCA ACGCTTCCGA AATCCAGATG GACCAGATGG AAGTGGATAC 
AAGCCAGTTG AACCAGGAAC CATTGATGAG AGAAGTGATG TTCTACAAAA ACGTTACAAG 
GAGCTTCTTG ATTTGGCTGC TGAACGTAAG CGTCGTCTCG AAGACAACAA GAGACTTTGC 
CAGTTCTGGT GGGATGTTGC TGAGCTTGAA CATGGTATTA AGGAACAAGA GCAAGTTCTT 
TCGTCCACTG ATACTGGCCG TGATATTGTC ACTGTTTCGC ATTTGTTGGC CAAACATAAG 
AACGCTGAGA ACAACCTTCG CGACTTGGAG AAGTACCTCG ATCGTCTTGA TGTTTCTGGA 
AAAGAATTGC AAGATGAAAG CATCCCAGGA AGTGACAACA TTCCTCCAAG GCTTGCCGAG 
ATTCGTGATT ACATCAACAA GCTCAAGGAA CTCTCTGCTT CTCGCAAAGA AAGACTTGCC 
GGTGGAGTCG AATACTACCA ATTCTTCACC GACGCCGACG ATGTCGATCG CTACTTGTAT 
GATACTCTCC GCGTCATGTC ATCCGAAGAC GTTGGAAAAG ACGAGGGAAC TGTTCAACTT 
CTCCTCAAGA AGCACGACGA TGTCCATGAC GAGCTCCAGA ACTTTGACCA ACACATCAAG 
GTTCTTCACG CCAAGGCCGA GTCTCTTCCA CAGGAAGCCA GAGAACATCC AGATATCAGA 
CAACGATTGG ACACAACTCT CAAGCAGAAG GCCGAACTCG AAAACCTTTC TCAACTTCGC 
AAGCAGAGAC TTATCGATGC TCTCTCCCTG TACAAGTTGT ACTCGGATGC CGATTCAGTC 
GAGTCTTGGA TCGATGAGAA GGGAAAACTT TTGGCAACAT TGGTCCCTGG ACGCGATATC 
GAAGAGGTTG AGATCATGAA GCATCGTTTC GATACTCTTG AGCAAGATAT GAAGAATCAA 
GAAGCTAAGG TTACTAACGT CAATGACTTG GCTCGTCAAT TGCTTAATGT GGAACATCCA 
AACTCCGACG ACATTCTTCA CCGTCAAAAC AAGCTCAACG CTCGCTGGGC TCAACTTCGC 
GACATGGTCG ATCAAAAGAG AAACGAGCTC GAGAGAGCTC ACCGCCTCGA AACTTTCCGC 
ATCGATTGCC AAGAAACAGT TACTTGGATC GAGGACAAGA CTCGTGTACT CGAGGATTCG 
GACGCGTTGA CCAACGATCT TTCCGGTGTC ATGAAACTTC AAAGAAGATT GTCCATGATG 
GAACGCGATT TGGGAGCGAT TCAAGCCAAG CTCGACTCGC TTCACAAAGA AGCCGATGAC 
ATCGAAAGAG AACGACCACA AGAAGCTCAA GCCATTCGTG AAGATATCAA GAGAATCCAC 
CAAGTCTGGG ATATCCTTAA CAAGAAAGTT CGTGAGCACG AAGCTAAACT CGATGAAGCT 
GGAGATCTTC AACGCTTCCT CCGTGATTTG GATCACTTCC AAGCCTGGCT TACTGCCACC 
CAGAGACAAG TTGCCTCAGA GGAAGAGCCA CAATCCTTGG CTGAAGCTGA GCAACTTCTC 
AATCAACACG CTGCCATCCG TGAAGAGATT GACGGATACG CTGAGGATTA CAAGAAGATG 
CGTGCAATGG GAGATCGTGT CACTCAAGAT CAAACCGATC CACAATACAT GTTCCTTCGT 
CAACGTCTTG CTGGACTTCA AGAAGGATGG GAAGAGTTGC AGCGAATGTG GGACAACAGA 
CAACATTTGC TTTCTCAAGG ACTCAACCTT CAAATGTTCC TTCGCGATGC CAAACAAGCA 
GAGGTTATGC TCTCTCAACA AGAAAACTAT CTCGCCAAGG ACGATATCCC ACAATCTTTG 
GAGCAAGCCG AGAACCAACT CAAGAGACAC CAAGACTTCA TCACCACTAT GGATGCCAAC 
GACGAAAAGA TTCGCGCCGT CGGAATGTTC GGTGATCAGC TCTGCCAAGA TGGTCACTAT 
GCCGCTGACA AGATCCATAA GAAGGCTAGA AACATTGATG AACGTCGTGG AGCCAACCGC 
GAGAAGGCTC AAGAAGTACT CAAGAAGCTG AAGGACGCCT TGTCTCTCCA ACAATTCTTG 
TCCGACTGCG ACGAGCTGAG AGAATGGATC GAGGAGAAGA TGATCCGTGC TCAAGATGAG 
ACTTACAGAG ACGCCAAGAC TATCACCTCC AAATTCGTCC GACATCAAGC ATTCCAATCC 
GAACTTGCTG CTAACAAGGA ACGTCTTGAC CAACTTAAAC ATGCTGCCAT CAACCTTGGT 
GACGACAAGC CAGAGTACCA CGGAACCATT GATCCACAGA TCGAGGAACT CGCTACTCAA 
TGGGATGAGT TGGAAAAGAC TACAGAGGAG AAGGGACAGA AGTTGTTTGA TGCTAACCGC 
CAACAACTTT ACGTACAAAG TATTGCTGAC ATGAAGGAAT GGGCAACCCA ACTTGAGAAC 
GAGATGACTC GTGAAGACCA ACCAGGAGAT CTTACCACTG TCAATGTGGC CATGCAAAAA 
CAACATTTGA TCGAAACTGA AATGATCAAG AAGGCTCAAC ACATTGATCA ACTCATGGAG 
ATGGAGCCAC AGCTCGAGGA ACTTCACCCA GACGAGCTTG AAAACATTAA GGCTCATCGT 
TTGGCAGTTC AAGAACAACT TCAAAGACTC CAGGCTCCAT TGGACGACCG ACGTAAGGCA 
TTGGAACGCA AGAAGGCCGC CTTCCAATTT GGTCGCGATG TTGATGACGA GAAATTGTGG 
ATTTCTGAGA GACTTGTTCT CGCTAAAGCT CAAAATCTTG GAGAAAGCCT TCCAGATTGT 
CATCGTCTCC AGAAGAACCT ACAACTTCTT TCCAATGAAA TCGACAACCA CGAACCATGG 
ATCAACCAGA TCTGTAATAA TGGTCAAGAG TTGATTGACG AAGGACATGC TAACGGACCA 
GCATTCGAGA AGAAGATTCA AGAACTGCGC AGTGCTTGGC AAGAGTTGAA GGAAGCCGTC 
AAAGACAGAA AGGGAGATCT TGGAGAAAGC GAGAAGGCTC ATCAATTCTT GTATGATTGC 
GGAGAGGCTG AAGCCTGGAT GAGCGAACAA GAATTGTACA TGATGCAAGA CGAAAGAGGA 
AAAGACGAGT TCTCAACCAA GAACCAAATC AAGAAACACG AACGCCTTCA ATCCGATATT 
GACAAATTTG CTGATACGAT CCGTGCTCTT GCCACCAAGG CTCACAAGTT CGTCGAAGAG 
AAGTCACCAC TCACCGAACA GATCCAAGTT CGTCAAGCTC AAATCGAGAA GCTCTACGCA 
GGTCTTCAAG ATTTGTCGAA GGAGCGCAGA AAGAGACTCG AAGAGACCTT GGAACTTTAC 
GCTCTTCATC GTGAAATCGA CGATCTTCTT CAATGGATCG CCGACAAGGA AGTTGTTGCA 
GGAAGTCAAG AGAACGGACA AGACTACGAA CACGTCCAAA TGCTTCAAGA GAGATTCCAA 
CAGTTTGCTC GTGACACCGA AAACATCGGA TCCGAACGTG TTGCCAATGC TAATGATGGA 
TGTGATACTC TCATTGGTCA CGGACACACT GACGCTCCGA CTATAGCATT GTGGAAGGAT 
TCGTTGAATG AGGCCTGGGA GAATCTTCTG GAGTTGATGG ACACTCGTGC CCAAATTCTC 
GAGGCTTCTA GACTTCTTCA CAAGTTCTAC CACGACTGCC GTGATTGCTT GTCAAGAATC 
ATGGAAAAGA CTCATGCTAT GCCTGATGAT CTTGGACGTG ACTCAAGCAG TGTTGGTGCA 
CTCTCCAGAA AACATCAGAA CTATCTCAAG GATATTGCTG CAATTGGAGA ACAAGTTGCT 
CAAATCGAAA GAGATGCCGC AGAGCTTCGA GATGGTTACG CTGGAGACAA GGCTCTTGAT 
ATCGGATCTC GCGAAAGCGA GGTTGTCAAG GCATGGAGGC ATCTCCGCGG ACTCTGTGAC 
GCCCGTACAT CTCGTCTCAT GGATACTTCT GATCTTTTCA AGTTCATGAA TATGGTCCGT 
GATTTGTTGT TGTGGATGGA TGAGGTGAAG AGAGAAATGA ACTCACAAGA ACGTCCGAAG 
GATGTGAGCG GAGTCGAGTT GCTCATGAAC AATCATCAAT CGCTCAAGGC TGAAATCGAT 
GCTCGCGAGG AGAACTTCAA CGCGTGCATC TCATTGGGAC GAGATCTGCT CAACAGAAAA 
CACTACGCAT CAAGTGAAAT CGAGAAGAAG CTGATCAAGT TGACAACCGA AAGAGCCGAA 
ATGATGCGCA GATGGGAAGA TCGTTGGGAA TATCTTCAGC TCATTCTGGA AGTGTACCAA 
TTCGCCCGCG ACGCTGCTGT CGCCGAATCT TGGCTCTTCG CCCAAGAACC ATATCTTATT 
TCGAAGGAAT ACGGTAGAAA CCTCGAAGAA ACCATCAAAC TCATCAAGAA ACACGAGGCG 
TTCGAGAAAT CGGCATTCGC ACAAGAAGAA CGTTTCTTGG CGCTCGAGAA ATTGACAACG 
TTCGAATTGA AAGAAACTCA ACACCGCGAG GAGGAGACCG CCAAGCGACG TGGTCCAGCT 
CATATTGGAA GTCCTTCTAG GTCAACTCCA GCCGCGGAAA CATCTTTCGG CGCCCAGGAT 
GATGGAGCCA AACAAGGAGA AGCATTCGAA GGAACGCTTA TCAGAAAGCA CACTTACGAG 
AGTCTCGACC GGAAAGCGGC TAACCGCTCA TGGGAGAAAC TTTACGCAGT GTTGCGGCAG 
AATGAACTGT CATTCTACAA AGATCCGAAG CATAGAGATG AATCTGTGCA TGGAGAGCCA 
CCAATGGCAC TGCCAGGATG CTCTGTCAAC GTGGCATCAG ACTATCAAAA GAAGAAGAAC 
GTACTGTCGC TGAGGTTACC CATCGGCGCC GAGTACCTAT TCCAATGCGG TAGCGAAGAA 
GACATGCAGC GATGGCTCAC CGAGTTGCAA GTCGCCACGG GGCAAGCCCA ACTGGAAGAG 
GCTAGTAGAT CACAGACGCT ACCAGCAGAA GGCAGTGCCA CAAAGAAGAA GGGAGGATTT 
TTCTCTCGAG GCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-70 ( NM_001269144.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001256073.1 Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

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CloneID	OCa347849
Clone ID Related Accession (Same CDS sequence)	NM_001269144.1 , NM_001269144.2
Accession Version	NM_001269144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Spectrin beta chain
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003283). On Sep 4, 2019 this sequence version replaced NM_001269144.1.

ATGTCGTCGG TGGATGACGA GCATCATCCT TCAATTGAAG TTGATATTCC TGACGTTTAT 
GAAAGTGCAT CTGACTGTGA AAAAACTGAA GACTGCACCT ATCTAGGATC TATTCTCAAG 
GCGAAAAAAT CATTGCGAAA AACCAAGAAA GTCGCTAGTC CTGGTATCTA TCCGGGCTCG 
GCGGGTGCTC TTTCATCTCT TTCAAATGGC CTAGCCGATG AGAATAGATC GCCAACTAGT 
GCTGAAGTTT CTGATTCAGT ATGTGGACAA AAATCTATAA ATTCAGTTGA CTTGAGGTTC 
AGAGGATTGA GAGATGAACG TGAACTCGTG CAAAAGAAAA CATTTACAAA ATGGGTCAAC 
TCACACTTGG TTCGAGTCAG CTGCAAAGTA CAAGACCTCT ACATGGATAT GCGAGACGGA 
AAAATGCTCC TCCGACTCCT CGCAGTTCTT TCCGGTGAAC GTCTGCCGAA ACCGACGCCG 
GGAAAAATGC GTATCCACTG TCTTGAGAAC GTTGAGAAAG GTTTGCAATT CTTGCGCAAT 
CAACACGTGC ATCTCGAAAA CTTGGGTTCA CACGATATCG TTGACGGAAA TTCACGCCTC 
ACGTTGGGTC TCATCTGGAC GATCATCCTT CGATTCCAGA TCCAAGATAT CACATTTGAA 
GACGCTGACA ATCACGAGAC ACGTTCAGCG AAGGAGGCCT TGTTACTGTG GTGCCAGATG 
AAAACTGCTG GATATCCAAA TGTCAACGTC AAGAACTTCT CAACCAGTTG GAGAGATGGT 
CTTGCATTCA ATGCACTTAT TCATAAACAT AGACCTGATC TCGTAGACTA CGATAATTTA 
CAAAAATCGA ATGCTCTTTA CAATTTGCAA TCTGCTTTTG ATACCGCAGA AAATCAATTG 
GGATTGGCCA AGTTCCTCGA TGCCGAAGAT GTGAACGTTG ACCAACCTGA TGAGAAATCC 
ATTATCACTT ATGTAGTTAC ATATTATCAT TACTTCAACA AACTGAAACA AGATAACATC 
CAAGGAAAAC GTATCGGAAA AGTGATTAAT GAGTTGATGG AGAACGACAA AATGATCAAT 
CGATACGAAA CTCTTTCATC TGATTTGCTC GAATGGATCA ATGCCAAGAT CCAACTTCTC 
AACGAACGTC ATTTTGAGAA TAATTTGGAA GGTGTTCAAA GACAGCTCAC CGAGTTCAAC 
GACTACCGTA CTCAGGAGAA ACCACCAAAG TTCGATGAAA AGGGAGAGCT CGAAGTACTT 
CTGTTCACTC TTCAATCAGC TATGCGTGCC AACAATCAGA GACCTTTCGT GCCGAGAGAA 
GGAAAACTGA TTGCTGATAT CAACCGAGCA TGGCAATCCC TTGAGAAGGC AGAACACGAA 
CGAGAACTTG TCCTGAAAGA GGAACTCATT CGTCAAGAGA AACTCGAACA ACTCGCAGCC 
CGATTCAACA GAAAGGCCGA GATGCGAGAG ACTTGGCTCA CAGAAAACCA ACGGCTTGTC 
AGTCAGGACA ATTTCGGAAA CGACTTGTCT TCGGTGGAAG CTGCCACCAA AAAGCACGAA 
GCCATCGAGA CGGACATCTT TGCTTACGAG GAGCGTGTTC AAGCCGTCGT CGCTGTTGCT 
GGAGAGCTTG AAGCCGAGAA CTACCATGAT CAAGCCAAAA TCAATGAGAG AAAGGAGAAT 
GTTCTTCAAC TCTGGAACTA TCTTTTCCAA CTTCTTTTGG CTCGCAGAGT TCGATTGGAG 
TTGTCAATGG CCATTCAAAA GATCTTCCAC GATATGCTTC TTACTCTCGA TCTCATGGAT 
GATATTAAGA GCAGACTTTT AAGCGAGGAT CTTGGAGCCC ATCTTATGGA CGTAGAAGAT 
TTGCTTCAAA AGCACGCTCT TCTTGAGTCT GATATTAATA TTATTGGAGA ACGAGTTAAC 
AATTCTATTG CTCAAGCTCA ACGCTTCCGA AATCCAGATG GACCAGATGG AAGTGGATAC 
AAGCCAGTTG AACCAGGAAC CATTGATGAG AGAAGTGATG TTCTACAAAA ACGTTACAAG 
GAGCTTCTTG ATTTGGCTGC TGAACGTAAG CGTCGTCTCG AAGACAACAA GAGACTTTGC 
CAGTTCTGGT GGGATGTTGC TGAGCTTGAA CATGGTATTA AGGAACAAGA GCAAGTTCTT 
TCGTCCACTG ATACTGGCCG TGATATTGTC ACTGTTTCGC ATTTGTTGGC CAAACATAAG 
AACGCTGAGA ACAACCTTCG CGACTTGGAG AAGTACCTCG ATCGTCTTGA TGTTTCTGGA 
AAAGAATTGC AAGATGAAAG CATCCCAGGA AGTGACAACA TTCCTCCAAG GCTTGCCGAG 
ATTCGTGATT ACATCAACAA GCTCAAGGAA CTCTCTGCTT CTCGCAAAGA AAGACTTGCC 
GGTGGAGTCG AATACTACCA ATTCTTCACC GACGCCGACG ATGTCGATCG CTACTTGTAT 
GATACTCTCC GCGTCATGTC ATCCGAAGAC GTTGGAAAAG ACGAGGGAAC TGTTCAACTT 
CTCCTCAAGA AGCACGACGA TGTCCATGAC GAGCTCCAGA ACTTTGACCA ACACATCAAG 
GTTCTTCACG CCAAGGCCGA GTCTCTTCCA CAGGAAGCCA GAGAACATCC AGATATCAGA 
CAACGATTGG ACACAACTCT CAAGCAGAAG GCCGAACTCG AAAACCTTTC TCAACTTCGC 
AAGCAGAGAC TTATCGATGC TCTCTCCCTG TACAAGTTGT ACTCGGATGC CGATTCAGTC 
GAGTCTTGGA TCGATGAGAA GGGAAAACTT TTGGCAACAT TGGTCCCTGG ACGCGATATC 
GAAGAGGTTG AGATCATGAA GCATCGTTTC GATACTCTTG AGCAAGATAT GAAGAATCAA 
GAAGCTAAGG TTACTAACGT CAATGACTTG GCTCGTCAAT TGCTTAATGT GGAACATCCA 
AACTCCGACG ACATTCTTCA CCGTCAAAAC AAGCTCAACG CTCGCTGGGC TCAACTTCGC 
GACATGGTCG ATCAAAAGAG AAACGAGCTC GAGAGAGCTC ACCGCCTCGA AACTTTCCGC 
ATCGATTGCC AAGAAACAGT TACTTGGATC GAGGACAAGA CTCGTGTACT CGAGGATTCG 
GACGCGTTGA CCAACGATCT TTCCGGTGTC ATGAAACTTC AAAGAAGATT GTCCATGATG 
GAACGCGATT TGGGAGCGAT TCAAGCCAAG CTCGACTCGC TTCACAAAGA AGCCGATGAC 
ATCGAAAGAG AACGACCACA AGAAGCTCAA GCCATTCGTG AAGATATCAA GAGAATCCAC 
CAAGTCTGGG ATATCCTTAA CAAGAAAGTT CGTGAGCACG AAGCTAAACT CGATGAAGCT 
GGAGATCTTC AACGCTTCCT CCGTGATTTG GATCACTTCC AAGCCTGGCT TACTGCCACC 
CAGAGACAAG TTGCCTCAGA GGAAGAGCCA CAATCCTTGG CTGAAGCTGA GCAACTTCTC 
AATCAACACG CTGCCATCCG TGAAGAGATT GACGGATACG CTGAGGATTA CAAGAAGATG 
CGTGCAATGG GAGATCGTGT CACTCAAGAT CAAACCGATC CACAATACAT GTTCCTTCGT 
CAACGTCTTG CTGGACTTCA AGAAGGATGG GAAGAGTTGC AGCGAATGTG GGACAACAGA 
CAACATTTGC TTTCTCAAGG ACTCAACCTT CAAATGTTCC TTCGCGATGC CAAACAAGCA 
GAGGTTATGC TCTCTCAACA AGAAAACTAT CTCGCCAAGG ACGATATCCC ACAATCTTTG 
GAGCAAGCCG AGAACCAACT CAAGAGACAC CAAGACTTCA TCACCACTAT GGATGCCAAC 
GACGAAAAGA TTCGCGCCGT CGGAATGTTC GGTGATCAGC TCTGCCAAGA TGGTCACTAT 
GCCGCTGACA AGATCCATAA GAAGGCTAGA AACATTGATG AACGTCGTGG AGCCAACCGC 
GAGAAGGCTC AAGAAGTACT CAAGAAGCTG AAGGACGCCT TGTCTCTCCA ACAATTCTTG 
TCCGACTGCG ACGAGCTGAG AGAATGGATC GAGGAGAAGA TGATCCGTGC TCAAGATGAG 
ACTTACAGAG ACGCCAAGAC TATCACCTCC AAATTCGTCC GACATCAAGC ATTCCAATCC 
GAACTTGCTG CTAACAAGGA ACGTCTTGAC CAACTTAAAC ATGCTGCCAT CAACCTTGGT 
GACGACAAGC CAGAGTACCA CGGAACCATT GATCCACAGA TCGAGGAACT CGCTACTCAA 
TGGGATGAGT TGGAAAAGAC TACAGAGGAG AAGGGACAGA AGTTGTTTGA TGCTAACCGC 
CAACAACTTT ACGTACAAAG TATTGCTGAC ATGAAGGAAT GGGCAACCCA ACTTGAGAAC 
GAGATGACTC GTGAAGACCA ACCAGGAGAT CTTACCACTG TCAATGTGGC CATGCAAAAA 
CAACATTTGA TCGAAACTGA AATGATCAAG AAGGCTCAAC ACATTGATCA ACTCATGGAG 
ATGGAGCCAC AGCTCGAGGA ACTTCACCCA GACGAGCTTG AAAACATTAA GGCTCATCGT 
TTGGCAGTTC AAGAACAACT TCAAAGACTC CAGGCTCCAT TGGACGACCG ACGTAAGGCA 
TTGGAACGCA AGAAGGCCGC CTTCCAATTT GGTCGCGATG TTGATGACGA GAAATTGTGG 
ATTTCTGAGA GACTTGTTCT CGCTAAAGCT CAAAATCTTG GAGAAAGCCT TCCAGATTGT 
CATCGTCTCC AGAAGAACCT ACAACTTCTT TCCAATGAAA TCGACAACCA CGAACCATGG 
ATCAACCAGA TCTGTAATAA TGGTCAAGAG TTGATTGACG AAGGACATGC TAACGGACCA 
GCATTCGAGA AGAAGATTCA AGAACTGCGC AGTGCTTGGC AAGAGTTGAA GGAAGCCGTC 
AAAGACAGAA AGGGAGATCT TGGAGAAAGC GAGAAGGCTC ATCAATTCTT GTATGATTGC 
GGAGAGGCTG AAGCCTGGAT GAGCGAACAA GAATTGTACA TGATGCAAGA CGAAAGAGGA 
AAAGACGAGT TCTCAACCAA GAACCAAATC AAGAAACACG AACGCCTTCA ATCCGATATT 
GACAAATTTG CTGATACGAT CCGTGCTCTT GCCACCAAGG CTCACAAGTT CGTCGAAGAG 
AAGTCACCAC TCACCGAACA GATCCAAGTT CGTCAAGCTC AAATCGAGAA GCTCTACGCA 
GGTCTTCAAG ATTTGTCGAA GGAGCGCAGA AAGAGACTCG AAGAGACCTT GGAACTTTAC 
GCTCTTCATC GTGAAATCGA CGATCTTCTT CAATGGATCG CCGACAAGGA AGTTGTTGCA 
GGAAGTCAAG AGAACGGACA AGACTACGAA CACGTCCAAA TGCTTCAAGA GAGATTCCAA 
CAGTTTGCTC GTGACACCGA AAACATCGGA TCCGAACGTG TTGCCAATGC TAATGATGGA 
TGTGATACTC TCATTGGTCA CGGACACACT GACGCTCCGA CTATAGCATT GTGGAAGGAT 
TCGTTGAATG AGGCCTGGGA GAATCTTCTG GAGTTGATGG ACACTCGTGC CCAAATTCTC 
GAGGCTTCTA GACTTCTTCA CAAGTTCTAC CACGACTGCC GTGATTGCTT GTCAAGAATC 
ATGGAAAAGA CTCATGCTAT GCCTGATGAT CTTGGACGTG ACTCAAGCAG TGTTGGTGCA 
CTCTCCAGAA AACATCAGAA CTATCTCAAG GATATTGCTG CAATTGGAGA ACAAGTTGCT 
CAAATCGAAA GAGATGCCGC AGAGCTTCGA GATGGTTACG CTGGAGACAA GGCTCTTGAT 
ATCGGATCTC GCGAAAGCGA GGTTGTCAAG GCATGGAGGC ATCTCCGCGG ACTCTGTGAC 
GCCCGTACAT CTCGTCTCAT GGATACTTCT GATCTTTTCA AGTTCATGAA TATGGTCCGT 
GATTTGTTGT TGTGGATGGA TGAGGTGAAG AGAGAAATGA ACTCACAAGA ACGTCCGAAG 
GATGTGAGCG GAGTCGAGTT GCTCATGAAC AATCATCAAT CGCTCAAGGC TGAAATCGAT 
GCTCGCGAGG AGAACTTCAA CGCGTGCATC TCATTGGGAC GAGATCTGCT CAACAGAAAA 
CACTACGCAT CAAGTGAAAT CGAGAAGAAG CTGATCAAGT TGACAACCGA AAGAGCCGAA 
ATGATGCGCA GATGGGAAGA TCGTTGGGAA TATCTTCAGC TCATTCTGGA AGTGTACCAA 
TTCGCCCGCG ACGCTGCTGT CGCCGAATCT TGGCTCTTCG CCCAAGAACC ATATCTTATT 
TCGAAGGAAT ACGGTAGAAA CCTCGAAGAA ACCATCAAAC TCATCAAGAA ACACGAGGCG 
TTCGAGAAAT CGGCATTCGC ACAAGAAGAA CGTTTCTTGG CGCTCGAGAA ATTGACAACG 
TTCGAATTGA AAGAAACTCA ACACCGCGAG GAGGAGACCG CCAAGCGACG TGGTCCAGCT 
CATATTGGAA GTCCTTCTAG GTCAACTCCA GCCGCGGAAA CATCTTTCGG CGCCCAGGAT 
GATGGAGCCA AACAAGGAGA AGCATTCGAA GGAACGCTTA TCAGAAAGCA CACTTACGAG 
AGTCTCGACC GGAAAGCGGC TAACCGCTCA TGGGAGAAAC TTTACGCAGT GTTGCGGCAG 
AATGAACTGT CATTCTACAA AGATCCGAAG CATAGAGATG AATCTGTGCA TGGAGAGCCA 
CCAATGGCAC TGCCAGGATG CTCTGTCAAC GTGGCATCAG ACTATCAAAA GAAGAAGAAC 
GTACTGTCGC TGAGGTTACC CATCGGCGCC GAGTACCTAT TCCAATGCGG TAGCGAAGAA 
GACATGCAGC GATGGCTCAC CGAGTTGCAA GTCGCCACGG GGCAAGCCCA ACTGGAAGAG 
GCTAGTAGAT CACAGACGCT ACCAGCAGAA GGCAGTGCCA CAAAGAAGAA GGGAGGATTT 
TTCTCTCGAG GCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001256073.1
CDS	62..7042
Translation	MSSVDDEHHPSIEVDIPDVYESASDCEKTEDCTYLGSILKAKKSLRKTKKVASPGIYPGSAGALSSLSNGLADENRSPTSAEVSDSVCGQKSINSVDLRFRGLRDERELVQKKTFTKWVNSHLVRVSCKVQDLYMDMRDGKMLLRLLAVLSGERLPKPTPGKMRIHCLENVEKGLQFLRNQHVHLENLGSHDIVDGNSRLTLGLIWTIILRFQIQDITFEDADNHETRSAKEALLLWCQMKTAGYPNVNVKNFSTSWRDGLAFNALIHKHRPDLVDYDNLQKSNALYNLQSAFDTAENQLGLAKFLDAEDVNVDQPDEKSIITYVVTYYHYFNKLKQDNIQGKRIGKVINELMENDKMINRYETLSSDLLEWINAKIQLLNERHFENNLEGVQRQLTEFNDYRTQEKPPKFDEKGELEVLLFTLQSAMRANNQRPFVPREGKLIADINRAWQSLEKAEHERELVLKEELIRQEKLEQLAARFNRKAEMRETWLTENQRLVSQDNFGNDLSSVEAATKKHEAIETDIFAYEERVQAVVAVAGELEAENYHDQAKINERKENVLQLWNYLFQLLLARRVRLELSMAIQKIFHDMLLTLDLMDDIKSRLLSEDLGAHLMDVEDLLQKHALLESDINIIGERVNNSIAQAQRFRNPDGPDGSGYKPVEPGTIDERSDVLQKRYKELLDLAAERKRRLEDNKRLCQFWWDVAELEHGIKEQEQVLSSTDTGRDIVTVSHLLAKHKNAENNLRDLEKYLDRLDVSGKELQDESIPGSDNIPPRLAEIRDYINKLKELSASRKERLAGGVEYYQFFTDADDVDRYLYDTLRVMSSEDVGKDEGTVQLLLKKHDDVHDELQNFDQHIKVLHAKAESLPQEAREHPDIRQRLDTTLKQKAELENLSQLRKQRLIDALSLYKLYSDADSVESWIDEKGKLLATLVPGRDIEEVEIMKHRFDTLEQDMKNQEAKVTNVNDLARQLLNVEHPNSDDILHRQNKLNARWAQLRDMVDQKRNELERAHRLETFRIDCQETVTWIEDKTRVLEDSDALTNDLSGVMKLQRRLSMMERDLGAIQAKLDSLHKEADDIERERPQEAQAIREDIKRIHQVWDILNKKVREHEAKLDEAGDLQRFLRDLDHFQAWLTATQRQVASEEEPQSLAEAEQLLNQHAAIREEIDGYAEDYKKMRAMGDRVTQDQTDPQYMFLRQRLAGLQEGWEELQRMWDNRQHLLSQGLNLQMFLRDAKQAEVMLSQQENYLAKDDIPQSLEQAENQLKRHQDFITTMDANDEKIRAVGMFGDQLCQDGHYAADKIHKKARNIDERRGANREKAQEVLKKLKDALSLQQFLSDCDELREWIEEKMIRAQDETYRDAKTITSKFVRHQAFQSELAANKERLDQLKHAAINLGDDKPEYHGTIDPQIEELATQWDELEKTTEEKGQKLFDANRQQLYVQSIADMKEWATQLENEMTREDQPGDLTTVNVAMQKQHLIETEMIKKAQHIDQLMEMEPQLEELHPDELENIKAHRLAVQEQLQRLQAPLDDRRKALERKKAAFQFGRDVDDEKLWISERLVLAKAQNLGESLPDCHRLQKNLQLLSNEIDNHEPWINQICNNGQELIDEGHANGPAFEKKIQELRSAWQELKEAVKDRKGDLGESEKAHQFLYDCGEAEAWMSEQELYMMQDERGKDEFSTKNQIKKHERLQSDIDKFADTIRALATKAHKFVEEKSPLTEQIQVRQAQIEKLYAGLQDLSKERRKRLEETLELYALHREIDDLLQWIADKEVVAGSQENGQDYEHVQMLQERFQQFARDTENIGSERVANANDGCDTLIGHGHTDAPTIALWKDSLNEAWENLLELMDTRAQILEASRLLHKFYHDCRDCLSRIMEKTHAMPDDLGRDSSSVGALSRKHQNYLKDIAAIGEQVAQIERDAAELRDGYAGDKALDIGSRESEVVKAWRHLRGLCDARTSRLMDTSDLFKFMNMVRDLLLWMDEVKREMNSQERPKDVSGVELLMNNHQSLKAEIDAREENFNACISLGRDLLNRKHYASSEIEKKLIKLTTERAEMMRRWEDRWEYLQLILEVYQFARDAAVAESWLFAQEPYLISKEYGRNLEETIKLIKKHEAFEKSAFAQEERFLALEKLTTFELKETQHREEETAKRRGPAHIGSPSRSTPAAETSFGAQDDGAKQGEAFEGTLIRKHTYESLDRKAANRSWEKLYAVLRQNELSFYKDPKHRDESVHGEPPMALPGCSVNVASDYQKKKNVLSLRLPIGAEYLFQCGSEEDMQRWLTELQVATGQAQLEEASRSQTLPAEGSATKKKGGFFSRGKK

Target ORF information:

RefSeq Version	NM_001269144.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Spectrin beta chain (unc-70), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001269144.2

ORF Insert Sequence:

ATGTCGTCGG TGGATGACGA GCATCATCCT TCAATTGAAG TTGATATTCC TGACGTTTAT 
GAAAGTGCAT CTGACTGTGA AAAAACTGAA GACTGCACCT ATCTAGGATC TATTCTCAAG 
GCGAAAAAAT CATTGCGAAA AACCAAGAAA GTCGCTAGTC CTGGTATCTA TCCGGGCTCG 
GCGGGTGCTC TTTCATCTCT TTCAAATGGC CTAGCCGATG AGAATAGATC GCCAACTAGT 
GCTGAAGTTT CTGATTCAGT ATGTGGACAA AAATCTATAA ATTCAGTTGA CTTGAGGTTC 
AGAGGATTGA GAGATGAACG TGAACTCGTG CAAAAGAAAA CATTTACAAA ATGGGTCAAC 
TCACACTTGG TTCGAGTCAG CTGCAAAGTA CAAGACCTCT ACATGGATAT GCGAGACGGA 
AAAATGCTCC TCCGACTCCT CGCAGTTCTT TCCGGTGAAC GTCTGCCGAA ACCGACGCCG 
GGAAAAATGC GTATCCACTG TCTTGAGAAC GTTGAGAAAG GTTTGCAATT CTTGCGCAAT 
CAACACGTGC ATCTCGAAAA CTTGGGTTCA CACGATATCG TTGACGGAAA TTCACGCCTC 
ACGTTGGGTC TCATCTGGAC GATCATCCTT CGATTCCAGA TCCAAGATAT CACATTTGAA 
GACGCTGACA ATCACGAGAC ACGTTCAGCG AAGGAGGCCT TGTTACTGTG GTGCCAGATG 
AAAACTGCTG GATATCCAAA TGTCAACGTC AAGAACTTCT CAACCAGTTG GAGAGATGGT 
CTTGCATTCA ATGCACTTAT TCATAAACAT AGACCTGATC TCGTAGACTA CGATAATTTA 
CAAAAATCGA ATGCTCTTTA CAATTTGCAA TCTGCTTTTG ATACCGCAGA AAATCAATTG 
GGATTGGCCA AGTTCCTCGA TGCCGAAGAT GTGAACGTTG ACCAACCTGA TGAGAAATCC 
ATTATCACTT ATGTAGTTAC ATATTATCAT TACTTCAACA AACTGAAACA AGATAACATC 
CAAGGAAAAC GTATCGGAAA AGTGATTAAT GAGTTGATGG AGAACGACAA AATGATCAAT 
CGATACGAAA CTCTTTCATC TGATTTGCTC GAATGGATCA ATGCCAAGAT CCAACTTCTC 
AACGAACGTC ATTTTGAGAA TAATTTGGAA GGTGTTCAAA GACAGCTCAC CGAGTTCAAC 
GACTACCGTA CTCAGGAGAA ACCACCAAAG TTCGATGAAA AGGGAGAGCT CGAAGTACTT 
CTGTTCACTC TTCAATCAGC TATGCGTGCC AACAATCAGA GACCTTTCGT GCCGAGAGAA 
GGAAAACTGA TTGCTGATAT CAACCGAGCA TGGCAATCCC TTGAGAAGGC AGAACACGAA 
CGAGAACTTG TCCTGAAAGA GGAACTCATT CGTCAAGAGA AACTCGAACA ACTCGCAGCC 
CGATTCAACA GAAAGGCCGA GATGCGAGAG ACTTGGCTCA CAGAAAACCA ACGGCTTGTC 
AGTCAGGACA ATTTCGGAAA CGACTTGTCT TCGGTGGAAG CTGCCACCAA AAAGCACGAA 
GCCATCGAGA CGGACATCTT TGCTTACGAG GAGCGTGTTC AAGCCGTCGT CGCTGTTGCT 
GGAGAGCTTG AAGCCGAGAA CTACCATGAT CAAGCCAAAA TCAATGAGAG AAAGGAGAAT 
GTTCTTCAAC TCTGGAACTA TCTTTTCCAA CTTCTTTTGG CTCGCAGAGT TCGATTGGAG 
TTGTCAATGG CCATTCAAAA GATCTTCCAC GATATGCTTC TTACTCTCGA TCTCATGGAT 
GATATTAAGA GCAGACTTTT AAGCGAGGAT CTTGGAGCCC ATCTTATGGA CGTAGAAGAT 
TTGCTTCAAA AGCACGCTCT TCTTGAGTCT GATATTAATA TTATTGGAGA ACGAGTTAAC 
AATTCTATTG CTCAAGCTCA ACGCTTCCGA AATCCAGATG GACCAGATGG AAGTGGATAC 
AAGCCAGTTG AACCAGGAAC CATTGATGAG AGAAGTGATG TTCTACAAAA ACGTTACAAG 
GAGCTTCTTG ATTTGGCTGC TGAACGTAAG CGTCGTCTCG AAGACAACAA GAGACTTTGC 
CAGTTCTGGT GGGATGTTGC TGAGCTTGAA CATGGTATTA AGGAACAAGA GCAAGTTCTT 
TCGTCCACTG ATACTGGCCG TGATATTGTC ACTGTTTCGC ATTTGTTGGC CAAACATAAG 
AACGCTGAGA ACAACCTTCG CGACTTGGAG AAGTACCTCG ATCGTCTTGA TGTTTCTGGA 
AAAGAATTGC AAGATGAAAG CATCCCAGGA AGTGACAACA TTCCTCCAAG GCTTGCCGAG 
ATTCGTGATT ACATCAACAA GCTCAAGGAA CTCTCTGCTT CTCGCAAAGA AAGACTTGCC 
GGTGGAGTCG AATACTACCA ATTCTTCACC GACGCCGACG ATGTCGATCG CTACTTGTAT 
GATACTCTCC GCGTCATGTC ATCCGAAGAC GTTGGAAAAG ACGAGGGAAC TGTTCAACTT 
CTCCTCAAGA AGCACGACGA TGTCCATGAC GAGCTCCAGA ACTTTGACCA ACACATCAAG 
GTTCTTCACG CCAAGGCCGA GTCTCTTCCA CAGGAAGCCA GAGAACATCC AGATATCAGA 
CAACGATTGG ACACAACTCT CAAGCAGAAG GCCGAACTCG AAAACCTTTC TCAACTTCGC 
AAGCAGAGAC TTATCGATGC TCTCTCCCTG TACAAGTTGT ACTCGGATGC CGATTCAGTC 
GAGTCTTGGA TCGATGAGAA GGGAAAACTT TTGGCAACAT TGGTCCCTGG ACGCGATATC 
GAAGAGGTTG AGATCATGAA GCATCGTTTC GATACTCTTG AGCAAGATAT GAAGAATCAA 
GAAGCTAAGG TTACTAACGT CAATGACTTG GCTCGTCAAT TGCTTAATGT GGAACATCCA 
AACTCCGACG ACATTCTTCA CCGTCAAAAC AAGCTCAACG CTCGCTGGGC TCAACTTCGC 
GACATGGTCG ATCAAAAGAG AAACGAGCTC GAGAGAGCTC ACCGCCTCGA AACTTTCCGC 
ATCGATTGCC AAGAAACAGT TACTTGGATC GAGGACAAGA CTCGTGTACT CGAGGATTCG 
GACGCGTTGA CCAACGATCT TTCCGGTGTC ATGAAACTTC AAAGAAGATT GTCCATGATG 
GAACGCGATT TGGGAGCGAT TCAAGCCAAG CTCGACTCGC TTCACAAAGA AGCCGATGAC 
ATCGAAAGAG AACGACCACA AGAAGCTCAA GCCATTCGTG AAGATATCAA GAGAATCCAC 
CAAGTCTGGG ATATCCTTAA CAAGAAAGTT CGTGAGCACG AAGCTAAACT CGATGAAGCT 
GGAGATCTTC AACGCTTCCT CCGTGATTTG GATCACTTCC AAGCCTGGCT TACTGCCACC 
CAGAGACAAG TTGCCTCAGA GGAAGAGCCA CAATCCTTGG CTGAAGCTGA GCAACTTCTC 
AATCAACACG CTGCCATCCG TGAAGAGATT GACGGATACG CTGAGGATTA CAAGAAGATG 
CGTGCAATGG GAGATCGTGT CACTCAAGAT CAAACCGATC CACAATACAT GTTCCTTCGT 
CAACGTCTTG CTGGACTTCA AGAAGGATGG GAAGAGTTGC AGCGAATGTG GGACAACAGA 
CAACATTTGC TTTCTCAAGG ACTCAACCTT CAAATGTTCC TTCGCGATGC CAAACAAGCA 
GAGGTTATGC TCTCTCAACA AGAAAACTAT CTCGCCAAGG ACGATATCCC ACAATCTTTG 
GAGCAAGCCG AGAACCAACT CAAGAGACAC CAAGACTTCA TCACCACTAT GGATGCCAAC 
GACGAAAAGA TTCGCGCCGT CGGAATGTTC GGTGATCAGC TCTGCCAAGA TGGTCACTAT 
GCCGCTGACA AGATCCATAA GAAGGCTAGA AACATTGATG AACGTCGTGG AGCCAACCGC 
GAGAAGGCTC AAGAAGTACT CAAGAAGCTG AAGGACGCCT TGTCTCTCCA ACAATTCTTG 
TCCGACTGCG ACGAGCTGAG AGAATGGATC GAGGAGAAGA TGATCCGTGC TCAAGATGAG 
ACTTACAGAG ACGCCAAGAC TATCACCTCC AAATTCGTCC GACATCAAGC ATTCCAATCC 
GAACTTGCTG CTAACAAGGA ACGTCTTGAC CAACTTAAAC ATGCTGCCAT CAACCTTGGT 
GACGACAAGC CAGAGTACCA CGGAACCATT GATCCACAGA TCGAGGAACT CGCTACTCAA 
TGGGATGAGT TGGAAAAGAC TACAGAGGAG AAGGGACAGA AGTTGTTTGA TGCTAACCGC 
CAACAACTTT ACGTACAAAG TATTGCTGAC ATGAAGGAAT GGGCAACCCA ACTTGAGAAC 
GAGATGACTC GTGAAGACCA ACCAGGAGAT CTTACCACTG TCAATGTGGC CATGCAAAAA 
CAACATTTGA TCGAAACTGA AATGATCAAG AAGGCTCAAC ACATTGATCA ACTCATGGAG 
ATGGAGCCAC AGCTCGAGGA ACTTCACCCA GACGAGCTTG AAAACATTAA GGCTCATCGT 
TTGGCAGTTC AAGAACAACT TCAAAGACTC CAGGCTCCAT TGGACGACCG ACGTAAGGCA 
TTGGAACGCA AGAAGGCCGC CTTCCAATTT GGTCGCGATG TTGATGACGA GAAATTGTGG 
ATTTCTGAGA GACTTGTTCT CGCTAAAGCT CAAAATCTTG GAGAAAGCCT TCCAGATTGT 
CATCGTCTCC AGAAGAACCT ACAACTTCTT TCCAATGAAA TCGACAACCA CGAACCATGG 
ATCAACCAGA TCTGTAATAA TGGTCAAGAG TTGATTGACG AAGGACATGC TAACGGACCA 
GCATTCGAGA AGAAGATTCA AGAACTGCGC AGTGCTTGGC AAGAGTTGAA GGAAGCCGTC 
AAAGACAGAA AGGGAGATCT TGGAGAAAGC GAGAAGGCTC ATCAATTCTT GTATGATTGC 
GGAGAGGCTG AAGCCTGGAT GAGCGAACAA GAATTGTACA TGATGCAAGA CGAAAGAGGA 
AAAGACGAGT TCTCAACCAA GAACCAAATC AAGAAACACG AACGCCTTCA ATCCGATATT 
GACAAATTTG CTGATACGAT CCGTGCTCTT GCCACCAAGG CTCACAAGTT CGTCGAAGAG 
AAGTCACCAC TCACCGAACA GATCCAAGTT CGTCAAGCTC AAATCGAGAA GCTCTACGCA 
GGTCTTCAAG ATTTGTCGAA GGAGCGCAGA AAGAGACTCG AAGAGACCTT GGAACTTTAC 
GCTCTTCATC GTGAAATCGA CGATCTTCTT CAATGGATCG CCGACAAGGA AGTTGTTGCA 
GGAAGTCAAG AGAACGGACA AGACTACGAA CACGTCCAAA TGCTTCAAGA GAGATTCCAA 
CAGTTTGCTC GTGACACCGA AAACATCGGA TCCGAACGTG TTGCCAATGC TAATGATGGA 
TGTGATACTC TCATTGGTCA CGGACACACT GACGCTCCGA CTATAGCATT GTGGAAGGAT 
TCGTTGAATG AGGCCTGGGA GAATCTTCTG GAGTTGATGG ACACTCGTGC CCAAATTCTC 
GAGGCTTCTA GACTTCTTCA CAAGTTCTAC CACGACTGCC GTGATTGCTT GTCAAGAATC 
ATGGAAAAGA CTCATGCTAT GCCTGATGAT CTTGGACGTG ACTCAAGCAG TGTTGGTGCA 
CTCTCCAGAA AACATCAGAA CTATCTCAAG GATATTGCTG CAATTGGAGA ACAAGTTGCT 
CAAATCGAAA GAGATGCCGC AGAGCTTCGA GATGGTTACG CTGGAGACAA GGCTCTTGAT 
ATCGGATCTC GCGAAAGCGA GGTTGTCAAG GCATGGAGGC ATCTCCGCGG ACTCTGTGAC 
GCCCGTACAT CTCGTCTCAT GGATACTTCT GATCTTTTCA AGTTCATGAA TATGGTCCGT 
GATTTGTTGT TGTGGATGGA TGAGGTGAAG AGAGAAATGA ACTCACAAGA ACGTCCGAAG 
GATGTGAGCG GAGTCGAGTT GCTCATGAAC AATCATCAAT CGCTCAAGGC TGAAATCGAT 
GCTCGCGAGG AGAACTTCAA CGCGTGCATC TCATTGGGAC GAGATCTGCT CAACAGAAAA 
CACTACGCAT CAAGTGAAAT CGAGAAGAAG CTGATCAAGT TGACAACCGA AAGAGCCGAA 
ATGATGCGCA GATGGGAAGA TCGTTGGGAA TATCTTCAGC TCATTCTGGA AGTGTACCAA 
TTCGCCCGCG ACGCTGCTGT CGCCGAATCT TGGCTCTTCG CCCAAGAACC ATATCTTATT 
TCGAAGGAAT ACGGTAGAAA CCTCGAAGAA ACCATCAAAC TCATCAAGAA ACACGAGGCG 
TTCGAGAAAT CGGCATTCGC ACAAGAAGAA CGTTTCTTGG CGCTCGAGAA ATTGACAACG 
TTCGAATTGA AAGAAACTCA ACACCGCGAG GAGGAGACCG CCAAGCGACG TGGTCCAGCT 
CATATTGGAA GTCCTTCTAG GTCAACTCCA GCCGCGGAAA CATCTTTCGG CGCCCAGGAT 
GATGGAGCCA AACAAGGAGA AGCATTCGAA GGAACGCTTA TCAGAAAGCA CACTTACGAG 
AGTCTCGACC GGAAAGCGGC TAACCGCTCA TGGGAGAAAC TTTACGCAGT GTTGCGGCAG 
AATGAACTGT CATTCTACAA AGATCCGAAG CATAGAGATG AATCTGTGCA TGGAGAGCCA 
CCAATGGCAC TGCCAGGATG CTCTGTCAAC GTGGCATCAG ACTATCAAAA GAAGAAGAAC 
GTACTGTCGC TGAGGTTACC CATCGGCGCC GAGTACCTAT TCCAATGCGG TAGCGAAGAA 
GACATGCAGC GATGGCTCAC CGAGTTGCAA GTCGCCACGG GGCAAGCCCA ACTGGAAGAG 
GCTAGTAGAT CACAGACGCT ACCAGCAGAA GGCAGTGCCA CAAAGAAGAA GGGAGGATTT 
TTCTCTCGAG GCAAGAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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unc-70 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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