SPTBN1 cDNA ORF clone, Canis lupus familiaris(dog)

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe13797 XM_022424749.1
Latest version!
Canis lupus familiaris spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe13797 XM_531827.5
Latest version!
Canis lupus familiaris spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe13798 XM_005626108.3
Latest version!
Canis lupus familiaris spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OCe13799 XM_003639327.4
Latest version!
Canis lupus familiaris spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OCe13799 XM_003639327.3 Canis lupus familiaris spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe13798 XM_005626108.2 Canis lupus familiaris spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe13797
    Clone ID Related Accession (Same CDS sequence) XM_531827.5 , XM_022424749.1
    Accession Version XM_531827.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_531827.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGATGT GGACGACTGG GACAACGAGA ACAGTTCTGC AAGGCTCTTC
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAGG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCA GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAAG TCCTCTCTGG GGAGAGGCTG
    CCTAAACCCA CCAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTCGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAAAATGC ATTTAACTTG
    GCAGAGCAGC ACCTTGGCCT CACTAAGCTG TTGGATCCAG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATTAT CACCTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG
    CTTCAGGCAT TCAACACCTA TCGCACTGTG GAGAAACCGC CCAAGTTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GAGCCAACAA TCAGAAGGTC
    TACATGCCCC GCGAGGGCAA ACTCATCTCT GACATTAATA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATTAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACCTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCTGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCGT ACGAAGAACG CGTGCAGGCT
    GTGGTGGCCG TGGCTAGGGA GCTTGAGGCA GAGAACTACC ATGACATCAA GCGCATCACA
    GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAGTACCTAC TGGAATTGCT CAAAGCCCGG
    AGGCAGCGTC TCGAGATGAA CCTGGGGTTG CAAAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTATCTC AAGACTATGG CAAACACTTG
    CTTGGCGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCCGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCTTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGT
    TACAAGCCCT GCGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAACTCT GCCAGTTGGC GGCTGAACGC CGGGCTCGTC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAAAT GGCAGAAGAG GAGGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG GAAGGACCTG ACCAGCGTCA TGCGTCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGCGGCCACT TCGAGCAGGC CATCAAAGAA
    GGTGAAGACA TGATCGCAGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GAGGATCCTT
    TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA TGCTTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAT GACGTGGGCC ACGACGAGTA CTCCACACAG
    TCTCTGGTCA AGAAACACAA GGATGTGGCA GAGGAGATCG CCAACTACAG GCCCACCATT
    GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCAGGATGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAACGG TACAAGGAGG TAGCAGAGCT GACGCGGCTG
    AGGAAGCAGG CGCTGCAGGA CACGCTCGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT TCCAGAAAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGG TTTGAGAGCC TTGAACCGGA AATGAATAAC
    CAGGCTTCCC GGGTCGCCGT GGTGAACCAG ATTGCACGGC AGCTCATGCA CAGTGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC
    CGAGAGCTGG TTGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC
    CACCTTGAGT GCAACGAAAC CAAATCCTGG ATTCGAGAGA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATTGAGGCG AAGCTCAGCG ACCTGCAGAA AGAGGCCGAG
    AAGCTCGAGT CTGAGCACCC TGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCCGG
    ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TCACTGAAGC TGAGAAGCTC
    CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGG TGGAATGAGC TCCACAAAAT GTGGGAGAAT
    CGGCAGAGTC TCCTCTCCCA GTCCCACGCC TACCAGCAGT TTCTTAGGGA CACCAAGCAG
    GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTGTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGACGCC
    AACGAGGAGA AGATTAATGC TGTTGTGGAG ACGGGCCGGA GGCTGGTGAG CGATGGAAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGAAAGAAT
    CGTGAGGCCG CCAGTGAGCT TCTGATGAGA TTGAAAGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAGGAGCT CTCTCTCTGG ATCAATGAAA AGATGCTTAC CGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTACAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCCGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAC
    ATGTGGGAAG TCCTTGAATC CACCACCCAG ACCAAGGCCC AGAGGCTCTT TGATGCAAAC
    AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTCAAA
    AAGCAACAGA TGCTGGAGAA CCAAATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG
    AGCCAGGCCC AGGCCCTGAG TCAGGAGGGA AAGAGCACCG ACGAGGTGGA TAGCAAGCGC
    CTCACGGTAC AGACCAAGTT CATGGAACTG CTGGAGCCCT TAAGCGAAAG AAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATTTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACTTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGACA CCAGCCTCGC
    ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAATGCT
    GAGGCCATCC GGCGGAGGCT TGCTGACCTC AAGGAGCTGT GGGGCCTCCT CATCGAGGAG
    ACAGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT
    GCGGCTGAGG CTGAAGCATG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC CGTCTCCATG TTAAAGAAGC ACCAGATTCT GGAGCAAGCC
    GTGGAGGACT ACGCAGAGAC AGTGCACCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT
    GACAGTCATC CTGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC
    GCCGGCCTGA AGGACCTTGC TGAGGAGCGG AGAGGCAAGC TGGACGAGAG GCACAGGCTC
    TTCCAGCTCA ACCGGGAGGT GGATGACCTG GAGCAGTGGA TCGCCGAGAG GGAGGTGGTC
    GCAGGGTCGC ACGAGCTGGG ACAGGACTAC GAACACGTCA CGATGTTACA AGAACGATTC
    CGGGAATTTG CCCGAGACAC AGGGAACATT GGGCAAGAGC GCGTGGACAC GGTCAATCAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCCG CCACCATTGC TGAGTGGAAG
    GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTAGAGCTCA TTGACACAAG AACACAGATT
    CTCGCTGCCT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGC
    ATACAGGACA AACACAAGAA ACTGCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTGCAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTAC AGGAGGACGC AGCCCGTCTC CAGGCAGCCT ATGCGGGTGA CAAGGCTGAT
    GACATCCAGA AGCGGGAGAA CGAGGTCCTG GAAGCCTGGA AAGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGAGACCTCA TGCTGTGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCACC AAGGCATCAA AGCTGAGATC
    GATGCTCGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GCTGGCGAGA
    AAGCACTATG CGTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA AAAGAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC
    CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGGCAGGA GCCATACCTA
    TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCTTTTGAAA AATCTGCAGC CACCTGGGAC GAGAGGTTCT CCGCCCTGGA GAGGCTGACA
    ACGTTGGAGT TATTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCCTCTCCCG AGCCGAGCAC GAAAGCTTCT GAGGATACTG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AGGTGAAATG GTCAACGGCG CCGCGGAACA GAGAACAAGC
    TCAAAGGAGT CCAGCCCCAT CCCCTCCCCG ACCTCGGACC GCAAAGCCAA GACTGCCCTC
    CCGGCCCAGA GTGCTGCCAC CTTGCCGGCC AGAACCCAGG AGACCCCTTC GGCCCAGATG
    GAAGGCTTCC TAAATCGGAA GCACGAGTGG GAGGCTCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACGGCTG CCTCTGGAAT CCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT GAGATTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT
    AGCCCCGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGCGGTT CAGCATTTTT
    GGCAAAAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_531827.2
    CDS72..7166
    Translation

    Target ORF information:

    RefSeq Version XM_531827.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_531827.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGATGT GGACGACTGG GACAACGAGA ACAGTTCTGC AAGGCTCTTC
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAGG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCA GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAAG TCCTCTCTGG GGAGAGGCTG
    CCTAAACCCA CCAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTCGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAAAATGC ATTTAACTTG
    GCAGAGCAGC ACCTTGGCCT CACTAAGCTG TTGGATCCAG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATTAT CACCTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG
    CTTCAGGCAT TCAACACCTA TCGCACTGTG GAGAAACCGC CCAAGTTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GAGCCAACAA TCAGAAGGTC
    TACATGCCCC GCGAGGGCAA ACTCATCTCT GACATTAATA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATTAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACCTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCTGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCGT ACGAAGAACG CGTGCAGGCT
    GTGGTGGCCG TGGCTAGGGA GCTTGAGGCA GAGAACTACC ATGACATCAA GCGCATCACA
    GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAGTACCTAC TGGAATTGCT CAAAGCCCGG
    AGGCAGCGTC TCGAGATGAA CCTGGGGTTG CAAAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTATCTC AAGACTATGG CAAACACTTG
    CTTGGCGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCCGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCTTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGT
    TACAAGCCCT GCGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAACTCT GCCAGTTGGC GGCTGAACGC CGGGCTCGTC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAAAT GGCAGAAGAG GAGGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG GAAGGACCTG ACCAGCGTCA TGCGTCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGCGGCCACT TCGAGCAGGC CATCAAAGAA
    GGTGAAGACA TGATCGCAGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GAGGATCCTT
    TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA TGCTTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAT GACGTGGGCC ACGACGAGTA CTCCACACAG
    TCTCTGGTCA AGAAACACAA GGATGTGGCA GAGGAGATCG CCAACTACAG GCCCACCATT
    GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCAGGATGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAACGG TACAAGGAGG TAGCAGAGCT GACGCGGCTG
    AGGAAGCAGG CGCTGCAGGA CACGCTCGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT TCCAGAAAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGG TTTGAGAGCC TTGAACCGGA AATGAATAAC
    CAGGCTTCCC GGGTCGCCGT GGTGAACCAG ATTGCACGGC AGCTCATGCA CAGTGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC
    CGAGAGCTGG TTGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC
    CACCTTGAGT GCAACGAAAC CAAATCCTGG ATTCGAGAGA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATTGAGGCG AAGCTCAGCG ACCTGCAGAA AGAGGCCGAG
    AAGCTCGAGT CTGAGCACCC TGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCCGG
    ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TCACTGAAGC TGAGAAGCTC
    CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGG TGGAATGAGC TCCACAAAAT GTGGGAGAAT
    CGGCAGAGTC TCCTCTCCCA GTCCCACGCC TACCAGCAGT TTCTTAGGGA CACCAAGCAG
    GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTGTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGACGCC
    AACGAGGAGA AGATTAATGC TGTTGTGGAG ACGGGCCGGA GGCTGGTGAG CGATGGAAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGAAAGAAT
    CGTGAGGCCG CCAGTGAGCT TCTGATGAGA TTGAAAGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAGGAGCT CTCTCTCTGG ATCAATGAAA AGATGCTTAC CGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTACAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCCGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAC
    ATGTGGGAAG TCCTTGAATC CACCACCCAG ACCAAGGCCC AGAGGCTCTT TGATGCAAAC
    AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTCAAA
    AAGCAACAGA TGCTGGAGAA CCAAATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG
    AGCCAGGCCC AGGCCCTGAG TCAGGAGGGA AAGAGCACCG ACGAGGTGGA TAGCAAGCGC
    CTCACGGTAC AGACCAAGTT CATGGAACTG CTGGAGCCCT TAAGCGAAAG AAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATTTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACTTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGACA CCAGCCTCGC
    ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAATGCT
    GAGGCCATCC GGCGGAGGCT TGCTGACCTC AAGGAGCTGT GGGGCCTCCT CATCGAGGAG
    ACAGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT
    GCGGCTGAGG CTGAAGCATG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC CGTCTCCATG TTAAAGAAGC ACCAGATTCT GGAGCAAGCC
    GTGGAGGACT ACGCAGAGAC AGTGCACCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT
    GACAGTCATC CTGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC
    GCCGGCCTGA AGGACCTTGC TGAGGAGCGG AGAGGCAAGC TGGACGAGAG GCACAGGCTC
    TTCCAGCTCA ACCGGGAGGT GGATGACCTG GAGCAGTGGA TCGCCGAGAG GGAGGTGGTC
    GCAGGGTCGC ACGAGCTGGG ACAGGACTAC GAACACGTCA CGATGTTACA AGAACGATTC
    CGGGAATTTG CCCGAGACAC AGGGAACATT GGGCAAGAGC GCGTGGACAC GGTCAATCAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCCG CCACCATTGC TGAGTGGAAG
    GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTAGAGCTCA TTGACACAAG AACACAGATT
    CTCGCTGCCT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGC
    ATACAGGACA AACACAAGAA ACTGCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTGCAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTAC AGGAGGACGC AGCCCGTCTC CAGGCAGCCT ATGCGGGTGA CAAGGCTGAT
    GACATCCAGA AGCGGGAGAA CGAGGTCCTG GAAGCCTGGA AAGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGAGACCTCA TGCTGTGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCACC AAGGCATCAA AGCTGAGATC
    GATGCTCGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GCTGGCGAGA
    AAGCACTATG CGTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA AAAGAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC
    CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGGCAGGA GCCATACCTA
    TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCTTTTGAAA AATCTGCAGC CACCTGGGAC GAGAGGTTCT CCGCCCTGGA GAGGCTGACA
    ACGTTGGAGT TATTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCCTCTCCCG AGCCGAGCAC GAAAGCTTCT GAGGATACTG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AGGTGAAATG GTCAACGGCG CCGCGGAACA GAGAACAAGC
    TCAAAGGAGT CCAGCCCCAT CCCCTCCCCG ACCTCGGACC GCAAAGCCAA GACTGCCCTC
    CCGGCCCAGA GTGCTGCCAC CTTGCCGGCC AGAACCCAGG AGACCCCTTC GGCCCAGATG
    GAAGGCTTCC TAAATCGGAA GCACGAGTGG GAGGCTCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACGGCTG CCTCTGGAAT CCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT GAGATTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT
    AGCCCCGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGCGGTT CAGCATTTTT
    GGCAAAAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13797
    Clone ID Related Accession (Same CDS sequence) XM_531827.5 , XM_022424749.1
    Accession Version XM_022424749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7095bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGATGT GGACGACTGG GACAACGAGA ACAGTTCTGC AAGGCTCTTC
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAGG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCA GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAAG TCCTCTCTGG GGAGAGGCTG
    CCTAAACCCA CCAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTCGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAAAATGC ATTTAACTTG
    GCAGAGCAGC ACCTTGGCCT CACTAAGCTG TTGGATCCAG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATTAT CACCTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG
    CTTCAGGCAT TCAACACCTA TCGCACTGTG GAGAAACCGC CCAAGTTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GAGCCAACAA TCAGAAGGTC
    TACATGCCCC GCGAGGGCAA ACTCATCTCT GACATTAATA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATTAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACCTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCTGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCGT ACGAAGAACG CGTGCAGGCT
    GTGGTGGCCG TGGCTAGGGA GCTTGAGGCA GAGAACTACC ATGACATCAA GCGCATCACA
    GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAGTACCTAC TGGAATTGCT CAAAGCCCGG
    AGGCAGCGTC TCGAGATGAA CCTGGGGTTG CAAAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTATCTC AAGACTATGG CAAACACTTG
    CTTGGCGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCCGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCTTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGT
    TACAAGCCCT GCGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAACTCT GCCAGTTGGC GGCTGAACGC CGGGCTCGTC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAAAT GGCAGAAGAG GAGGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG GAAGGACCTG ACCAGCGTCA TGCGTCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGCGGCCACT TCGAGCAGGC CATCAAAGAA
    GGTGAAGACA TGATCGCAGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GAGGATCCTT
    TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA TGCTTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAT GACGTGGGCC ACGACGAGTA CTCCACACAG
    TCTCTGGTCA AGAAACACAA GGATGTGGCA GAGGAGATCG CCAACTACAG GCCCACCATT
    GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCAGGATGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAACGG TACAAGGAGG TAGCAGAGCT GACGCGGCTG
    AGGAAGCAGG CGCTGCAGGA CACGCTCGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT TCCAGAAAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGG TTTGAGAGCC TTGAACCGGA AATGAATAAC
    CAGGCTTCCC GGGTCGCCGT GGTGAACCAG ATTGCACGGC AGCTCATGCA CAGTGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC
    CGAGAGCTGG TTGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC
    CACCTTGAGT GCAACGAAAC CAAATCCTGG ATTCGAGAGA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATTGAGGCG AAGCTCAGCG ACCTGCAGAA AGAGGCCGAG
    AAGCTCGAGT CTGAGCACCC TGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCCGG
    ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TCACTGAAGC TGAGAAGCTC
    CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGG TGGAATGAGC TCCACAAAAT GTGGGAGAAT
    CGGCAGAGTC TCCTCTCCCA GTCCCACGCC TACCAGCAGT TTCTTAGGGA CACCAAGCAG
    GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTGTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGACGCC
    AACGAGGAGA AGATTAATGC TGTTGTGGAG ACGGGCCGGA GGCTGGTGAG CGATGGAAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGAAAGAAT
    CGTGAGGCCG CCAGTGAGCT TCTGATGAGA TTGAAAGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAGGAGCT CTCTCTCTGG ATCAATGAAA AGATGCTTAC CGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTACAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCCGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAC
    ATGTGGGAAG TCCTTGAATC CACCACCCAG ACCAAGGCCC AGAGGCTCTT TGATGCAAAC
    AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTCAAA
    AAGCAACAGA TGCTGGAGAA CCAAATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG
    AGCCAGGCCC AGGCCCTGAG TCAGGAGGGA AAGAGCACCG ACGAGGTGGA TAGCAAGCGC
    CTCACGGTAC AGACCAAGTT CATGGAACTG CTGGAGCCCT TAAGCGAAAG AAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATTTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACTTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGACA CCAGCCTCGC
    ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAATGCT
    GAGGCCATCC GGCGGAGGCT TGCTGACCTC AAGGAGCTGT GGGGCCTCCT CATCGAGGAG
    ACAGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT
    GCGGCTGAGG CTGAAGCATG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC CGTCTCCATG TTAAAGAAGC ACCAGATTCT GGAGCAAGCC
    GTGGAGGACT ACGCAGAGAC AGTGCACCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT
    GACAGTCATC CTGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC
    GCCGGCCTGA AGGACCTTGC TGAGGAGCGG AGAGGCAAGC TGGACGAGAG GCACAGGCTC
    TTCCAGCTCA ACCGGGAGGT GGATGACCTG GAGCAGTGGA TCGCCGAGAG GGAGGTGGTC
    GCAGGGTCGC ACGAGCTGGG ACAGGACTAC GAACACGTCA CGATGTTACA AGAACGATTC
    CGGGAATTTG CCCGAGACAC AGGGAACATT GGGCAAGAGC GCGTGGACAC GGTCAATCAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCCG CCACCATTGC TGAGTGGAAG
    GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTAGAGCTCA TTGACACAAG AACACAGATT
    CTCGCTGCCT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGC
    ATACAGGACA AACACAAGAA ACTGCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTGCAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTAC AGGAGGACGC AGCCCGTCTC CAGGCAGCCT ATGCGGGTGA CAAGGCTGAT
    GACATCCAGA AGCGGGAGAA CGAGGTCCTG GAAGCCTGGA AAGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGAGACCTCA TGCTGTGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCACC AAGGCATCAA AGCTGAGATC
    GATGCTCGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GCTGGCGAGA
    AAGCACTATG CGTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA AAAGAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC
    CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGGCAGGA GCCATACCTA
    TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCTTTTGAAA AATCTGCAGC CACCTGGGAC GAGAGGTTCT CCGCCCTGGA GAGGCTGACA
    ACGTTGGAGT TATTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCCTCTCCCG AGCCGAGCAC GAAAGCTTCT GAGGATACTG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AGGTGAAATG GTCAACGGCG CCGCGGAACA GAGAACAAGC
    TCAAAGGAGT CCAGCCCCAT CCCCTCCCCG ACCTCGGACC GCAAAGCCAA GACTGCCCTC
    CCGGCCCAGA GTGCTGCCAC CTTGCCGGCC AGAACCCAGG AGACCCCTTC GGCCCAGATG
    GAAGGCTTCC TAAATCGGAA GCACGAGTGG GAGGCTCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACGGCTG CCTCTGGAAT CCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT GAGATTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT
    AGCCCCGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGCGGTT CAGCATTTTT
    GGCAAAAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022280457.1
    CDS1..7095
    Translation

    Target ORF information:

    RefSeq Version XM_022424749.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022424749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAACAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGATGT GGACGACTGG GACAACGAGA ACAGTTCTGC AAGGCTCTTC
    GAGCGGTCCC GCATCAAGGC TCTGGCAGAT GAGCGTGAGG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAATTCCCA CCTTGCCCGA GTGTCCTGCA GGATCACAGA CCTGTACACT
    GACCTTCGAG ATGGACGGAT GCTCATCAAG CTGCTGGAAG TCCTCTCTGG GGAGAGGCTG
    CCTAAACCCA CCAAGGGACG GATGCGCATC CACTGTCTGG AGAATGTGGA CAAAGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GCTCCCATGA CATTGTCGAT
    GGGAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCATTGCTG
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCGAACGTCA ACATTCACAA TTTCACCACT
    AGCTGGAGGG ACGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACCTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAAAATGC ATTTAACTTG
    GCAGAGCAGC ACCTTGGCCT CACTAAGCTG TTGGATCCAG AAGATATCAG TGTGGACCAC
    CCTGATGAGA AGTCTATTAT CACCTACGTG GTGACTTATT ACCACTACTT CTCTAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAATGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCACTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAATTCTC TGGTTGGGGT TCAACAACAG
    CTTCAGGCAT TCAACACCTA TCGCACTGTG GAGAAACCGC CCAAGTTTAC TGAGAAGGGG
    AACTTGGAAG TGCTGCTTTT TACTATTCAG AGCAAGATGA GAGCCAACAA TCAGAAGGTC
    TACATGCCCC GCGAGGGCAA ACTCATCTCT GACATTAATA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCTCTG CGGAACGAGC TCATTAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGATCGCAAG GCAGCTATGA GGGAGACCTG GCTGAGCGAA
    AACCAGCGTC TGGTGTCTCA GGACAACTTT GGGTTTGACC TTCCTGCAGT TGAGGCTGCC
    ACGAAAAAGC ACGAGGCCAT TGAGACAGAC ATTGCCGCGT ACGAAGAACG CGTGCAGGCT
    GTGGTGGCCG TGGCTAGGGA GCTTGAGGCA GAGAACTACC ATGACATCAA GCGCATCACA
    GCAAGGAAGG ACAATGTCAT CCGACTCTGG GAGTACCTAC TGGAATTGCT CAAAGCCCGG
    AGGCAGCGTC TCGAGATGAA CCTGGGGTTG CAAAAGATAT TCCAGGAAAT GCTCTACATT
    ATGGACTGGA TGGACGAAAT GAAGGTGCTA TTACTATCTC AAGACTATGG CAAACACTTG
    CTTGGCGTGG AAGACCTGTT ACAGAAGCAC GCCCTGGTTG AAGCCGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAATGCTTCT GCCCAGAAGT TTGCAACAGA TGGGGAAGGT
    TACAAGCCCT GCGACCCTCA GGTGATCCGA GACCGCGTGG CCCACATGGA GTTCTGTTAT
    CAAGAACTCT GCCAGTTGGC GGCTGAACGC CGGGCTCGTC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAAAT GGCAGAAGAG GAGGGCTGGA TACGGGAGAA GGAGAAGATC
    CTGTCCTCTG ACGACTACGG GAAGGACCTG ACCAGCGTCA TGCGTCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGCGGCCACT TCGAGCAGGC CATCAAAGAA
    GGTGAAGACA TGATCGCAGA GGAGCACTTT GGGTCAGAGA AGATCCGAGA GAGGATCCTT
    TACATCCGGG AGCAGTGGGC CAACCTGGAG CAGCTCTCGG CCATTCGGAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCCG ACGACATCGA TGCTTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAT GACGTGGGCC ACGACGAGTA CTCCACACAG
    TCTCTGGTCA AGAAACACAA GGATGTGGCA GAGGAGATCG CCAACTACAG GCCCACCATT
    GACACGCTGC ACGAGCAGGC CAGCGCCCTC CCCCAGGAGC ATGCCGAGTC TCAGGATGTG
    AGGGGCAGGC TGTCGGGCAT CGAGGAACGG TACAAGGAGG TAGCAGAGCT GACGCGGCTG
    AGGAAGCAGG CGCTGCAGGA CACGCTCGCC TTGTACAAGA TGTTCAGTGA GGCTGATGCC
    TGTGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT TCCAGAAAAG
    CTGGAGGACC TGGAAGTCAT CCAGCACAGG TTTGAGAGCC TTGAACCGGA AATGAATAAC
    CAGGCTTCCC GGGTCGCCGT GGTGAACCAG ATTGCACGGC AGCTCATGCA CAGTGGCCAC
    CCAAGTGAGA AGGAAATCAA AGCCCAGCAG GACAAACTCA ACACGAGGTG GAGTCAGTTC
    CGAGAGCTGG TTGACAGGAA GAAGGACGCT CTTCTCTCTG CCCTGAGCAT ACAGAACTAC
    CACCTTGAGT GCAACGAAAC CAAATCCTGG ATTCGAGAGA AGACCAAGGT CATCGAGTCC
    ACCCAGGACC TGGGCAATGA CCTGGCTGGC GTCATGGCCC TGCAGCGCAA GCTGACCGGC
    ATGGAGCGGG ACCTGGTGGC CATTGAGGCG AAGCTCAGCG ACCTGCAGAA AGAGGCCGAG
    AAGCTCGAGT CTGAGCACCC TGACCAGGCT CAGGCCATCC TGTCTCGGCT GGCCGAGATC
    AGCGACGTGT GGGAGGAGAT GAAGACCACC CTGAAAAACC GCGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGACGACT TCCAGTCCTG GCTCTCCCGG
    ACCCAGACGG CCATCGCCTC TGAGGACATG CCCAACACCC TCACTGAAGC TGAGAAGCTC
    CTCACGCAGC ATGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGACAGACCG ACGCCCAGTA CATGTTTCTG
    CGGCAGCGGC TGCAGGCCCT GGACACTGGG TGGAATGAGC TCCACAAAAT GTGGGAGAAT
    CGGCAGAGTC TCCTCTCCCA GTCCCACGCC TACCAGCAGT TTCTTAGGGA CACCAAGCAG
    GCCGAGGCCT TCCTTAACAA CCAGGAGTAT GTGTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGACT TCATGACCAC CATGGACGCC
    AACGAGGAGA AGATTAATGC TGTTGTGGAG ACGGGCCGGA GGCTGGTGAG CGATGGAAAC
    ATCAACTCAG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA TAGAAAGAAT
    CGTGAGGCCG CCAGTGAGCT TCTGATGAGA TTGAAAGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGATT GTCAGGAGCT CTCTCTCTGG ATCAATGAAA AGATGCTTAC CGCCCAGGAC
    ATGTCTTATG ATGAAGCCAG AAATCTACAC AGTAAATGGT TAAAACATCA AGCATTTATG
    GCAGAACTTG CATCCAACAA AGAATGGCTT GACAAAATTG AGAAGGAAGG AATGCAGCTC
    ATTTCAGAAA AGCCCGAGAC GGAAGCTGTG GTGAAGGAGA AACTCACTGG TTTACATAAC
    ATGTGGGAAG TCCTTGAATC CACCACCCAG ACCAAGGCCC AGAGGCTCTT TGATGCAAAC
    AAGGCTGAAC TTTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA CGGCCTGGAG
    AGTCAGATTC AGTCTGATGA CTATGGCAAA GACCTTACCA GCGTCAACAT CCTGCTCAAA
    AAGCAACAGA TGCTGGAGAA CCAAATGGAG GTGCGGAAGA AGGAGATCGA GGAGCTCCAG
    AGCCAGGCCC AGGCCCTGAG TCAGGAGGGA AAGAGCACCG ACGAGGTGGA TAGCAAGCGC
    CTCACGGTAC AGACCAAGTT CATGGAACTG CTGGAGCCCT TAAGCGAAAG AAAGCATAAC
    CTGCTGGCCT CCAAAGAGAT CCATCAGTTC AACAGGGATG TGGAGGACGA GATTTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACTTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGCTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGACA CCAGCCTCGC
    ATTGATGACA TCTTTGAGAG GAGCCAGAAC ATTGTCACCG ACAGCAGCAG CCTCAATGCT
    GAGGCCATCC GGCGGAGGCT TGCTGACCTC AAGGAGCTGT GGGGCCTCCT CATCGAGGAG
    ACAGAGAAGC GCCACAGGCG GCTTGAGGAG GCGCACAGGG CCCAGCAGTA CTACTTCGAT
    GCGGCTGAGG CTGAAGCATG GATGAGTGAG CAGGAGCTGT ACATGATGTC TGAGGAGAAG
    GCCAAGGATG AGCAGAGTGC CGTCTCCATG TTAAAGAAGC ACCAGATTCT GGAGCAAGCC
    GTGGAGGACT ACGCAGAGAC AGTGCACCAG CTCTCTAAGA CCAGCCGGGC CCTGGTGGCT
    GACAGTCATC CTGAAAGTGA GCGCATCAGC ATGCGGCAGT CCAAAGTGGA CAAGCTGTAC
    GCCGGCCTGA AGGACCTTGC TGAGGAGCGG AGAGGCAAGC TGGACGAGAG GCACAGGCTC
    TTCCAGCTCA ACCGGGAGGT GGATGACCTG GAGCAGTGGA TCGCCGAGAG GGAGGTGGTC
    GCAGGGTCGC ACGAGCTGGG ACAGGACTAC GAACACGTCA CGATGTTACA AGAACGATTC
    CGGGAATTTG CCCGAGACAC AGGGAACATT GGGCAAGAGC GCGTGGACAC GGTCAATCAC
    ATGGCAGATG AACTCATCAA CTCTGGACAT TCAGATGCCG CCACCATTGC TGAGTGGAAG
    GATGGGCTCA ACGAAGCCTG GGCTGACCTC CTAGAGCTCA TTGACACAAG AACACAGATT
    CTCGCTGCCT CCTATGAACT GCACAAGTTT TACCACGATG CCAAGGAGAT CTTTGGGCGC
    ATACAGGACA AACACAAGAA ACTGCCCGAG GAGCTTGGGA GAGATCAGAA CACAGTGGAG
    ACCTTGCAGA GAATGCACAC TACATTTGAG CATGACATCC AGGCTCTGGG CACTCAGGTG
    AGGCAGCTAC AGGAGGACGC AGCCCGTCTC CAGGCAGCCT ATGCGGGTGA CAAGGCTGAT
    GACATCCAGA AGCGGGAGAA CGAGGTCCTG GAAGCCTGGA AAGCCCTGCT GGATGCCTGT
    GAGGGCCGCA GGGTGCGGCT GGTGGACACG GGGGACAAGT TCCGCTTCTT CAGCATGGTG
    CGAGACCTCA TGCTGTGGAT GGAGGATGTC ATCCGGCAGA TCGAAGCCCA GGAGAAGCCA
    AGGGATGTAT CATCTGTTGA ACTATTAATG AATAATCACC AAGGCATCAA AGCTGAGATC
    GATGCTCGTA ATGACAGTTT CACAACCTGC ATTGAACTTG GGAAATCCCT GCTGGCGAGA
    AAGCACTATG CGTCTGAGGA GATCAAGGAA AAGTTACTGC AGTTGACGGA AAAGAGAAAA
    GAAATGATTG ACAAGTGGGA AGACCGATGG GAATGGTTAA GACTGATTTT GGAGGTCCAC
    CAGTTCTCAC GGGACGCCAG TGTGGCTGAG GCCTGGCTGC TCGGGCAGGA GCCATACCTA
    TCCAGCCGAG AAATAGGCCA GAGTGTGGAC GAGGTGGAGA AGCTCATCAA GCGCCACGAG
    GCTTTTGAAA AATCTGCAGC CACCTGGGAC GAGAGGTTCT CCGCCCTGGA GAGGCTGACA
    ACGTTGGAGT TATTGGAAGT GCGCAGACAG CAAGAGGAAG AGGAGAGGAA GAGGCGGCCG
    CCCTCTCCCG AGCCGAGCAC GAAAGCTTCT GAGGATACTG AGTCCCAGCA GCAGTGGGAT
    ACTTCAAAAG GAGAGCAAGT TTCCCAGAAC GGTTTGCCGG CTGAACAGGG ATCTCCACGG
    ATGGCAGAAA CGGTGGACAC AGGTGAAATG GTCAACGGCG CCGCGGAACA GAGAACAAGC
    TCAAAGGAGT CCAGCCCCAT CCCCTCCCCG ACCTCGGACC GCAAAGCCAA GACTGCCCTC
    CCGGCCCAGA GTGCTGCCAC CTTGCCGGCC AGAACCCAGG AGACCCCTTC GGCCCAGATG
    GAAGGCTTCC TAAATCGGAA GCACGAGTGG GAGGCTCACA ACAAGAAAGC CTCAAGCAGG
    TCCTGGCACA ATGTTTATTG TGTCATAAAT AACCAAGAAA TGGGTTTCTA CAAAGATGCA
    AAGACGGCTG CCTCTGGAAT CCCCTACCAC AGCGAGGTCC CTGTGAGTTT GAAAGAAGCC
    ATCTGTGAAG TGGCCCTTGA TTACAAGAAG AAGAAACACG TGTTCAAGCT GAGATTAAAT
    GATGGCAATG AGTACCTCTT CCAAGCCAAA GATGATGAGG AAATGAACAC ATGGATCCAG
    GCCATCTCCT CCGCCATCTC CTCTGATAAA CACGAGGTGT CCGCCAGCAC CCAGAGCACG
    CCAGCATCCA GCCGGGCACA GACCTTGCCC ACCAGCGTCG TCACCATCAC CAGCGAGTCT
    AGCCCCGGCA AGCGGGAGAA GGACAAAGAG AAAGACAAGG AGAAGCGGTT CAGCATTTTT
    GGCAAAAAGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13798
    Clone ID Related Accession (Same CDS sequence) XM_005626108.3 , XM_005626108.2
    Accession Version XM_005626108.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626108.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAGGTGAAAT GGTCAACGGC
    GCCGCGGAAC AGAGAACAAG CTCAAAGGAG TCCAGCCCCA TCCCCTCCCC GACCTCGGAC
    CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCTGCCA CCTTGCCGGC CAGAACCCAG
    GAGACCCCTT CGGCCCAGAT GGAAGGCTTC CTAAATCGGA AGCACGAGTG GGAGGCTCAC
    AACAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACGGCT GCCTCTGGAA TCCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC
    GTGTTCAAGC TGAGATTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGATGATGAG
    GAAATGAACA CATGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCCGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCAC AGACCTTGCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC TAGCCCCGGC AAGCGGGAGA AGGACAAAGA GAAAGACAAG
    GAGAAGCGGT TCAGCATTTT TGGCAAAAAG AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626165.1
    CDS54..7109
    Translation

    Target ORF information:

    RefSeq Version XM_005626108.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626108.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAGGTGAAAT GGTCAACGGC
    GCCGCGGAAC AGAGAACAAG CTCAAAGGAG TCCAGCCCCA TCCCCTCCCC GACCTCGGAC
    CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCTGCCA CCTTGCCGGC CAGAACCCAG
    GAGACCCCTT CGGCCCAGAT GGAAGGCTTC CTAAATCGGA AGCACGAGTG GGAGGCTCAC
    AACAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACGGCT GCCTCTGGAA TCCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC
    GTGTTCAAGC TGAGATTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGATGATGAG
    GAAATGAACA CATGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCCGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCAC AGACCTTGCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC TAGCCCCGGC AAGCGGGAGA AGGACAAAGA GAAAGACAAG
    GAGAAGCGGT TCAGCATTTT TGGCAAAAAG AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13798
    Clone ID Related Accession (Same CDS sequence) XM_005626108.3 , XM_005626108.2
    Accession Version XM_005626108.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAGGTGAAAT GGTCAACGGC
    GCCGCGGAAC AGAGAACAAG CTCAAAGGAG TCCAGCCCCA TCCCCTCCCC GACCTCGGAC
    CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCTGCCA CCTTGCCGGC CAGAACCCAG
    GAGACCCCTT CGGCCCAGAT GGAAGGCTTC CTAAATCGGA AGCACGAGTG GGAGGCTCAC
    AACAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACGGCT GCCTCTGGAA TCCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC
    GTGTTCAAGC TGAGATTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGATGATGAG
    GAAATGAACA CATGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCCGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCAC AGACCTTGCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC TAGCCCCGGC AAGCGGGAGA AGGACAAAGA GAAAGACAAG
    GAGAAGCGGT TCAGCATTTT TGGCAAAAAG AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626165.1
    CDS286..7341
    Translation

    Target ORF information:

    RefSeq Version XM_005626108.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626108.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GATGGCAGAA ACGGTGGACA CAGGTGAAAT GGTCAACGGC
    GCCGCGGAAC AGAGAACAAG CTCAAAGGAG TCCAGCCCCA TCCCCTCCCC GACCTCGGAC
    CGCAAAGCCA AGACTGCCCT CCCGGCCCAG AGTGCTGCCA CCTTGCCGGC CAGAACCCAG
    GAGACCCCTT CGGCCCAGAT GGAAGGCTTC CTAAATCGGA AGCACGAGTG GGAGGCTCAC
    AACAAGAAAG CCTCAAGCAG GTCCTGGCAC AATGTTTATT GTGTCATAAA TAACCAAGAA
    ATGGGTTTCT ACAAAGATGC AAAGACGGCT GCCTCTGGAA TCCCCTACCA CAGCGAGGTC
    CCTGTGAGTT TGAAAGAAGC CATCTGTGAA GTGGCCCTTG ATTACAAGAA GAAGAAACAC
    GTGTTCAAGC TGAGATTAAA TGATGGCAAT GAGTACCTCT TCCAAGCCAA AGATGATGAG
    GAAATGAACA CATGGATCCA GGCCATCTCC TCCGCCATCT CCTCTGATAA ACACGAGGTG
    TCCGCCAGCA CCCAGAGCAC GCCAGCATCC AGCCGGGCAC AGACCTTGCC CACCAGCGTC
    GTCACCATCA CCAGCGAGTC TAGCCCCGGC AAGCGGGAGA AGGACAAAGA GAAAGACAAG
    GAGAAGCGGT TCAGCATTTT TGGCAAAAAG AAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13799
    Clone ID Related Accession (Same CDS sequence) XM_003639327.3 , XM_003639327.4
    Accession Version XM_003639327.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product spectrin beta chain, non-erythrocytic 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003639327.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC
    TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003639375.1
    CDS1..6468
    Translation

    Target ORF information:

    RefSeq Version XM_003639327.3
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris spectrin, beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003639327.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC
    TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe13799
    Clone ID Related Accession (Same CDS sequence) XM_003639327.3 , XM_003639327.4
    Accession Version XM_003639327.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6468bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product spectrin beta chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003639327.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC
    TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003639375.1
    CDS1..6468
    Translation

    Target ORF information:

    RefSeq Version XM_003639327.4
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003639327.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC CAGCATCTCC GGGCCGCTGT CGCCCGCGTA CACGGGGCAG 
    GTGCCTTACA ACTACAACCA GCTGGAAGGG AGATTCAAGC AGCTTCAAGA TGAGCGTGAG
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAATTCCC ACCTTGCCCG AGTGTCCTGC
    AGGATCACAG ACCTGTACAC TGACCTTCGA GATGGACGGA TGCTCATCAA GCTGCTGGAA
    GTCCTCTCTG GGGAGAGGCT GCCTAAACCC ACCAAGGGAC GGATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAAGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGCTCCCATG ACATTGTCGA TGGGAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCATTGCT GTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCGAACGTC
    AACATTCACA ATTTCACCAC TAGCTGGAGG GACGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACCTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAAAATG CATTTAACTT GGCAGAGCAG CACCTTGGCC TCACTAAGCT GTTGGATCCA
    GAAGATATCA GTGTGGACCA CCCTGATGAG AAGTCTATTA TCACCTACGT GGTGACTTAT
    TACCACTACT TCTCTAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAATG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCACT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACCATCATT ATCTTGAACA ATCGCAAATT TGCCAATTCT
    CTGGTTGGGG TTCAACAACA GCTTCAGGCA TTCAACACCT ATCGCACTGT GGAGAAACCG
    CCCAAGTTTA CTGAGAAGGG GAACTTGGAA GTGCTGCTTT TTACTATTCA GAGCAAGATG
    AGAGCCAACA ATCAGAAGGT CTACATGCCC CGCGAGGGCA AACTCATCTC TGACATTAAT
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCTCT GCGGAACGAG
    CTCATTAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGATCGCAA GGCAGCTATG
    AGGGAGACCT GGCTGAGCGA AAACCAGCGT CTGGTGTCTC AGGACAACTT TGGGTTTGAC
    CTTCCTGCAG TTGAGGCTGC CACGAAAAAG CACGAGGCCA TTGAGACAGA CATTGCCGCG
    TACGAAGAAC GCGTGCAGGC TGTGGTGGCC GTGGCTAGGG AGCTTGAGGC AGAGAACTAC
    CATGACATCA AGCGCATCAC AGCAAGGAAG GACAATGTCA TCCGACTCTG GGAGTACCTA
    CTGGAATTGC TCAAAGCCCG GAGGCAGCGT CTCGAGATGA ACCTGGGGTT GCAAAAGATA
    TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGACGAAA TGAAGGTGCT ATTACTATCT
    CAAGACTATG GCAAACACTT GCTTGGCGTG GAAGACCTGT TACAGAAGCA CGCCCTGGTT
    GAAGCCGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAATGCTTC TGCCCAGAAG
    TTTGCAACAG ATGGGGAAGG TTACAAGCCC TGCGACCCTC AGGTGATCCG AGACCGCGTG
    GCCCACATGG AGTTCTGTTA TCAAGAACTC TGCCAGTTGG CGGCTGAACG CCGGGCTCGT
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAAA TGGCAGAAGA GGAGGGCTGG
    ATACGGGAGA AGGAGAAGAT CCTGTCCTCT GACGACTACG GGAAGGACCT GACCAGCGTC
    ATGCGTCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGCGGCCAC
    TTCGAGCAGG CCATCAAAGA AGGTGAAGAC ATGATCGCAG AGGAGCACTT TGGGTCAGAG
    AAGATCCGAG AGAGGATCCT TTACATCCGG GAGCAGTGGG CCAACCTGGA GCAGCTCTCG
    GCCATTCGGA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCC
    GACGACATCG ATGCTTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA TGACGTGGGC
    CACGACGAGT ACTCCACACA GTCTCTGGTC AAGAAACACA AGGATGTGGC AGAGGAGATC
    GCCAACTACA GGCCCACCAT TGACACGCTG CACGAGCAGG CCAGCGCCCT CCCCCAGGAG
    CATGCCGAGT CTCAGGATGT GAGGGGCAGG CTGTCGGGCA TCGAGGAACG GTACAAGGAG
    GTAGCAGAGC TGACGCGGCT GAGGAAGCAG GCGCTGCAGG ACACGCTCGC CTTGTACAAG
    ATGTTCAGTG AGGCTGATGC CTGTGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TTCCAGAAAA GCTGGAGGAC CTGGAAGTCA TCCAGCACAG GTTTGAGAGC
    CTTGAACCGG AAATGAATAA CCAGGCTTCC CGGGTCGCCG TGGTGAACCA GATTGCACGG
    CAGCTCATGC ACAGTGGCCA CCCAAGTGAG AAGGAAATCA AAGCCCAGCA GGACAAACTC
    AACACGAGGT GGAGTCAGTT CCGAGAGCTG GTTGACAGGA AGAAGGACGC TCTTCTCTCT
    GCCCTGAGCA TACAGAACTA CCACCTTGAG TGCAACGAAA CCAAATCCTG GATTCGAGAG
    AAGACCAAGG TCATCGAGTC CACCCAGGAC CTGGGCAATG ACCTGGCTGG CGTCATGGCC
    CTGCAGCGCA AGCTGACCGG CATGGAGCGG GACCTGGTGG CCATTGAGGC GAAGCTCAGC
    GACCTGCAGA AAGAGGCCGA GAAGCTCGAG TCTGAGCACC CTGACCAGGC TCAGGCCATC
    CTGTCTCGGC TGGCCGAGAT CAGCGACGTG TGGGAGGAGA TGAAGACCAC CCTGAAAAAC
    CGCGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGACGAC
    TTCCAGTCCT GGCTCTCCCG GACCCAGACG GCCATCGCCT CTGAGGACAT GCCCAACACC
    CTCACTGAAG CTGAGAAGCT CCTCACGCAG CATGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGACAGACC
    GACGCCCAGT ACATGTTTCT GCGGCAGCGG CTGCAGGCCC TGGACACTGG GTGGAATGAG
    CTCCACAAAA TGTGGGAGAA TCGGCAGAGT CTCCTCTCCC AGTCCCACGC CTACCAGCAG
    TTTCTTAGGG ACACCAAGCA GGCCGAGGCC TTCCTTAACA ACCAGGAGTA TGTGTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAC
    TTCATGACCA CCATGGACGC CAACGAGGAG AAGATTAATG CTGTTGTGGA GACGGGCCGG
    AGGCTGGTGA GCGATGGAAA CATCAACTCA GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ATAGAAAGAA TCGTGAGGCC GCCAGTGAGC TTCTGATGAG ATTGAAAGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAT TGTCAGGAGC TCTCTCTCTG GATCAATGAA
    AAGATGCTTA CCGCCCAGGA CATGTCTTAT GATGAAGCCA GAAATCTACA CAGTAAATGG
    TTAAAACATC AAGCATTTAT GGCAGAACTT GCATCCAACA AAGAATGGCT TGACAAAATT
    GAGAAGGAAG GAATGCAGCT CATTTCAGAA AAGCCCGAGA CGGAAGCTGT GGTGAAGGAG
    AAACTCACTG GTTTACATAA CATGTGGGAA GTCCTTGAAT CCACCACCCA GACCAAGGCC
    CAGAGGCTCT TTGATGCAAA CAAGGCTGAA CTTTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ACGGCCTGGA GAGTCAGATT CAGTCTGATG ACTATGGCAA AGACCTTACC
    AGCGTCAACA TCCTGCTCAA AAAGCAACAG ATGCTGGAGA ACCAAATGGA GGTGCGGAAG
    AAGGAGATCG AGGAGCTCCA GAGCCAGGCC CAGGCCCTGA GTCAGGAGGG AAAGAGCACC
    GACGAGGTGG ATAGCAAGCG CCTCACGGTA CAGACCAAGT TCATGGAACT GCTGGAGCCC
    TTAAGCGAAA GAAAGCATAA CCTGCTGGCC TCCAAAGAGA TCCATCAGTT CAACAGGGAT
    GTGGAGGACG AGATTTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACTTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGCTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGAC ACCAGCCTCG CATTGATGAC ATCTTTGAGA GGAGCCAGAA CATTGTCACC
    GACAGCAGCA GCCTCAATGC TGAGGCCATC CGGCGGAGGC TTGCTGACCT CAAGGAGCTG
    TGGGGCCTCC TCATCGAGGA GACAGAGAAG CGCCACAGGC GGCTTGAGGA GGCGCACAGG
    GCCCAGCAGT ACTACTTCGA TGCGGCTGAG GCTGAAGCAT GGATGAGTGA GCAGGAGCTG
    TACATGATGT CTGAGGAGAA GGCCAAGGAT GAGCAGAGTG CCGTCTCCAT GTTAAAGAAG
    CACCAGATTC TGGAGCAAGC CGTGGAGGAC TACGCAGAGA CAGTGCACCA GCTCTCTAAG
    ACCAGCCGGG CCCTGGTGGC TGACAGTCAT CCTGAAAGTG AGCGCATCAG CATGCGGCAG
    TCCAAAGTGG ACAAGCTGTA CGCCGGCCTG AAGGACCTTG CTGAGGAGCG GAGAGGCAAG
    CTGGACGAGA GGCACAGGCT CTTCCAGCTC AACCGGGAGG TGGATGACCT GGAGCAGTGG
    ATCGCCGAGA GGGAGGTGGT CGCAGGGTCG CACGAGCTGG GACAGGACTA CGAACACGTC
    ACGATGTTAC AAGAACGATT CCGGGAATTT GCCCGAGACA CAGGGAACAT TGGGCAAGAG
    CGCGTGGACA CGGTCAATCA CATGGCAGAT GAACTCATCA ACTCTGGACA TTCAGATGCC
    GCCACCATTG CTGAGTGGAA GGATGGGCTC AACGAAGCCT GGGCTGACCT CCTAGAGCTC
    ATTGACACAA GAACACAGAT TCTCGCTGCC TCCTATGAAC TGCACAAGTT TTACCACGAT
    GCCAAGGAGA TCTTTGGGCG CATACAGGAC AAACACAAGA AACTGCCCGA GGAGCTTGGG
    AGAGATCAGA ACACAGTGGA GACCTTGCAG AGAATGCACA CTACATTTGA GCATGACATC
    CAGGCTCTGG GCACTCAGGT GAGGCAGCTA CAGGAGGACG CAGCCCGTCT CCAGGCAGCC
    TATGCGGGTG ACAAGGCTGA TGACATCCAG AAGCGGGAGA ACGAGGTCCT GGAAGCCTGG
    AAAGCCCTGC TGGATGCCTG TGAGGGCCGC AGGGTGCGGC TGGTGGACAC GGGGGACAAG
    TTCCGCTTCT TCAGCATGGT GCGAGACCTC ATGCTGTGGA TGGAGGATGT CATCCGGCAG
    ATCGAAGCCC AGGAGAAGCC AAGGGATGTA TCATCTGTTG AACTATTAAT GAATAATCAC
    CAAGGCATCA AAGCTGAGAT CGATGCTCGT AATGACAGTT TCACAACCTG CATTGAACTT
    GGGAAATCCC TGCTGGCGAG AAAGCACTAT GCGTCTGAGG AGATCAAGGA AAAGTTACTG
    CAGTTGACGG AAAAGAGAAA AGAAATGATT GACAAGTGGG AAGACCGATG GGAATGGTTA
    AGACTGATTT TGGAGGTCCA CCAGTTCTCA CGGGACGCCA GTGTGGCTGA GGCCTGGCTG
    CTCGGGCAGG AGCCATACCT ATCCAGCCGA GAAATAGGCC AGAGTGTGGA CGAGGTGGAG
    AAGCTCATCA AGCGCCACGA GGCTTTTGAA AAATCTGCAG CCACCTGGGA CGAGAGGTTC
    TCCGCCCTGG AGAGGCTGAC AACGTTGGAG TTATTGGAAG TGCGCAGACA GCAAGAGGAA
    GAGGAGAGGA AGAGGCGGCC GCCCTCTCCC GAGCCGAGCA CGAAAGCTTC TGAGGATACT
    GAGTCCCAGC AGCAGTGGGA TACTTCAAAA GGAGAGCAAG TTTCCCAGAA CGGTTTGCCG
    GCTGAACAGG GATCTCCACG GGTTAGTTAC CGCTCTCAAA CCTACCAAAA CTACAAAAAC
    TTTAATAGCA GACGGACAGC CAGTGACCAG CCGTGGTCTG GACTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.