SPTBN1 cDNA ORF clone, Bos taurus(cattle)

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa23931 NM_001192276.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_024998774.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_024998773.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_015473439.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_005212632.2
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_005212631.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_024998775.1
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23932 XM_005212633.4
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23933 XM_005212634.3
Latest version!
Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa23931 XM_015473439.1 Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23932 XM_005212633.3 Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
OBa23931 XM_005212632.1 Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1371.30
$1959.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version NM_001192276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000011.1. On Jul 17, 2010 this sequence version replaced XM_588094.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571260.49110.1, SRR5571258.2549.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179205.1
    CDS107..7198
    Translation

    Target ORF information:

    RefSeq Version NM_001192276.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_015473439.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328925.1
    CDS290..7381
    Translation

    Target ORF information:

    RefSeq Version XM_015473439.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473439.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_005212632.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212689.1
    CDS134..7225
    Translation

    Target ORF information:

    RefSeq Version XM_005212632.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212632.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_015473439.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328925.1
    CDS333..7424
    Translation

    Target ORF information:

    RefSeq Version XM_015473439.2
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473439.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_005212631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212688.1
    CDS282..7373
    Translation

    Target ORF information:

    RefSeq Version XM_005212631.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_024998774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854542.1
    CDS97..7188
    Translation

    Target ORF information:

    RefSeq Version XM_024998774.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_024998775.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854543.1
    CDS211..7302
    Translation

    Target ORF information:

    RefSeq Version XM_024998775.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_005212632.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212689.1
    CDS483..7574
    Translation

    Target ORF information:

    RefSeq Version XM_005212632.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212632.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23931
    Clone ID Related Accession (Same CDS sequence) XM_015473439.1 , XM_005212632.2 , XM_015473439.2 , XM_005212631.1 , XM_024998774.1 , XM_024998775.1 , XM_005212632.1 , NM_001192276.1 , XM_024998773.1
    Accession Version XM_024998773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024854541.1
    CDS288..7379
    Translation

    Target ORF information:

    RefSeq Version XM_024998773.1
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024998773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGACGACCA CAGTAGCCAC AGACTATGAC AACATTGAGA TCCAGCAGCA GTACAGCGAT 
    GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAGA ACAGCTCCGC ACGGCTCTTT
    GAGCGGTCGC GCATCAAGGC TCTGGCAGAT GAACGTGAAG CTGTGCAAAA GAAGACCTTC
    ACCAAGTGGG TCAACTCCCA CCTTGCCCGG GTGTCCTGCC GGATCACAGA TCTGTACACT
    GACCTTCGGG ATGGACGGAT GCTCATCAAG CTACTGGAGG TCCTCTCTGG AGAGAGACTG
    CCTAAACCCA CCAAGGGACG AATGCGCATC CACTGTCTGG AGAATGTGGA CAAGGCCCTT
    CAGTTCCTGA AGGAGCAGAG AGTCCATCTT GAGAACATGG GGTCCCACGA CATCGTGGAT
    GGAAACCACC GGCTGACCCT CGGCCTCATC TGGACCATCA TCCTGCGCTT CCAGATCCAG
    GATATCAGTG TAGAAACTGA AGACAACAAA GAGAAGAAAT CTGCCAAGGA TGCTTTGCTC
    TTGTGGTGCC AGATGAAGAC AGCTGGGTAC CCCAACGTCA ATATTCACAA TTTCACAACC
    AGCTGGAGGG ATGGCATGGC CTTCAATGCA CTGATACACA AACACCGGCC TGACTTGATA
    GATTTTGACA AACTAAAGAA ATCCAATGCA CACTACAATC TGCAGAACGC CTTTAACCTG
    GCAGAGCAAC ACCTTGGCCT CACTAAACTC TTGGACCCCG AAGATATCAG TGTGGACCAT
    CCTGATGAGA AGTCTATTAT CACTTACGTG GTGACTTACT ACCACTACTT CTCAAAGATG
    AAGGCCTTGG CTGTTGAAGG AAAACGAATT GGAAAGGTGC TTGACAACGC TATTGAAACA
    GAAAAAATGA TTGAAAAGTA TGAGTCCCTT GCCTCTGACC TTCTGGAGTG GATTGAACAA
    ACTATCATTA TTTTGAACAA TCGCAAATTT GCCAACTCAC TCGTTGGGGT CCAGCAGCAG
    CTTCAGGCGT TCAACACTTA CCGCACTGTG GAGAAGCCCC CCAAATTTAC TGAGAAGGGG
    AACTTGGAAG TCCTGCTTTT CACCATTCAG AGCAAGATGA GGGCCAATAA CCAGAAGGTC
    TACATGCCCC GGGAGGGGAA GCTCATCTCT GACATTAACA AGGCCTGGGA AAGACTGGAA
    AAAGCAGAAC ACGAGCGAGA ACTGGCCCTG CGAAATGAGC TCATAAGACA GGAGAAACTG
    GAACAGCTCG CCCGCAGATT TGACCGCAAG GCAGCTATGA GAGAGACCTG GCTGAGCGAG
    AACCAGCGTC TGGTGTCCCA GGACAACTTT GGATTTGACC TTCCTGCTGT TGAGGCCGCC
    ACGAAAAAGC ATGAAGCCAT TGAGACTGAC ATCGCTGCAT ACGAGGAGCG CGTGCAGGCT
    GTGGTGGCTG TGGCCAGGGA ACTGGAGGCA GAGAACTACC ACGACATCAA GCGCATCACA
    GCACGGAAGG ACAACGTCAT CCGGCTCTGG GAGTACCTGC TGGAGCTGCT CAGGGCCCGG
    AGACAGCGCC TCGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTGTACATC
    ATGGACTGGA TGGATGAAAT GAAGGTGCTG TTATTGTCTC AAGACTATGG CAAACACTTG
    CTCGGCGTGG AAGACCTGTT ACAGAAGCAT GCCCTGGTTG AAGCAGACAT TGGCATCCAG
    GCAGAGCGGG TGAGAGGTGT CAACGCTTCT GCCCAGAAGT TTGCAACCGA CGGGGAAGGT
    TACAAGCCCT GCGACCCCCA GGTAATCCGA GACAGGGTGG CCCACATGGA GTTCTGTTAC
    CAAGAGCTTT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAAGAGTC CCGTCGCCTC
    TGGAAGTTCT TCTGGGAGAT GGCAGAGGAG GAGGGCTGGA TCCGGGAGAA GGAGAAGATC
    CTGTCCTCTG ATGACTACGG CAAGGACCTC ACAAGCGTCA TGCGCCTGCT CAGCAAGCAC
    CGGGCATTCG AGGATGAGAT GAGCGGCCGC AGTGGCCACT TCGAGCAAGC CATCAAGGAG
    GGCGAAGACA TGATCGCCGA GGAGCACTTC GGGTCAGAGA AGATCCGGGA GCGGATTGCC
    TACATCCGCG AGCAGTGGGC CCACCTGGAG CAGCTCTCGG CCATCCGCAA GAAGCGCCTG
    GAGGAGGCCT CGCTGCTGCA CCAGTTCCAG GCGGATGCTG ACGACATCGA TGCCTGGATG
    CTGGACATCC TCAAGATCGT CTCCAGCAAC GACGTGGGCC ACGATGAGTA CTCCACCCAG
    TCCCTGGTCA AGAAGCACAA GGACGTGGCG GAGGAGATCG CCAACTACCG GCCCACCATC
    GACTCGCTGC ACGAGCAAGC CAGCGCCCTG CCCCAGGAGC ACGCCGAGTC CCCCGACGTG
    CAGGGCCGGC TGGCGGGCAT TGAGGAGCGC TACAAGGAGG TGGCCGAGCT GACGCGGCTG
    CGGAAGCAGG CGCTGCAGGA CACTCTGGCC CTGTACAAGA TGTTCAGCGA GGCCGATGCC
    TGTGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA ACATGCAGAT CCCAGAGAAG
    CTGGAAGACC TGGAGGTCAT CCAGCACAGA TTCGAGAGCC TCGAACCGGA GATGAACAAC
    CAGGCTTCCC GGGTGGCAGT GGTGAACCAG ATTGCCCGGC AGCTGATGCA CAGCGGCCAC
    CCAAGTGAGA AGGAGATCAA AGCCCAGCAG GACAAGCTCA ACACGAGGTG GAGCCAGTTC
    AGGGAGCTGG TGGACCGGAA GAAGGATGCT CTCCTCTCCG CCCTGAGCAT CCAGAACTAC
    CACCTCGAGT GCAACGAAAC CAAGTCATGG ATCCGGGAGA AGACCAAAGT CATCGAATCC
    ACCCAGGACC TGGGCAACGA CCTGGCGGGT GTCATGGCCC TGCAGCGCAA GCTGACTGGC
    ATGGAGCGGG ACCTCGTGGC CATCGAGGCG AAACTGAGTG ACCTGCAGAA GGAGGCCGAG
    AAGCTGGAGT CCGAGCACCC CGACCAGGCC CAGGCTATCC TGTCTCGGCT GGCCGAGATC
    AGTGACGTGT GGGAGGAGAT GAAGACCACG CTGAAGAACC GTGAGGCCTC CCTGGGAGAG
    GCCAGCAAGC TGCAGCAGTT CCTGCGGGAC CTGGATGACT TCCAGTCCTG GCTCTCCAGG
    ACACAGACGG CCATTGCCTC GGAGGACATG CCTAACACCC TGACCGAGGC TGAGAAGCTC
    CTCACGCAGC ACGAGAACAT CAAAAACGAG ATCGACAACT ACGAGGAGGA CTACCAGAAG
    ATGAGGGACA TGGGCGAGAT GGTCACCCAG GGCCAGACAG ATGCCCAGTA CATGTTCCTG
    CGGCAGCGGC TGCAGGCCCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC
    AGGCAGAGCC TCCTCTCCCA GTCCCACGCT CACCAGCAGT TCCTGCGGGA CACCAAGCAG
    GCCGAAGCCT TCCTGAACAA CCAGGAGTAT GTTTTGGCCC ATACTGAAAT GCCCACCACC
    TTGGAAGGAG CTGAAGCAGC TATTAAAAAA CAAGAGGATT TCATGACCAC CATGGATGCC
    AATGAGGAGA AAATCAACGC GGTTGTGGAA ACGGGCCGGA GGCTGGTGAG CGATGGGAAC
    ATCAACTCGG ATCGCATCCA GGAGAAGGTG GACTCCATTG ATGACAGACA CAGGAAGAAT
    CGCGAGGCAG CCAGTGAGCT TCTGATGAGA CTGAAGGACA ACAGGGATCT TCAGAAATTC
    CTGCAAGACT GTCAGGAGCT GTCTCTCTGG ATCAATGAAA AGATGCTTAC AGCCCAGGAC
    ATGTCTTACG ATGAAGCCAG AAATCTGCAC AGTAAATGGT TGAAGCATCA AGCCTTTATG
    GCAGAACTTG CTTCCAACAA AGAATGGCTT GACAAAATCG AGAAGGAGGG AATGCAGCTC
    ATTTCAGAAA AGCCAGAGAC AGAAGCCGTG GTGAAGGAAA AACTCACAGG TTTACATAAA
    ATGTGGGAAG TCCTTGAATC CACCACACAG ACCAAGGCCC AGCGGCTCTT CGATGCGAAC
    AAAGCCGAAC TCTTCACCCA GAGCTGTGCA GATCTGGACA AATGGCTGCA TGGCCTGGAG
    AGTCAGATTC AGTCTGACGA CTATGGCAAA GACCTCACCA GCGTCAACAT CCTTCTGAAA
    AAGCAGCAGA TGCTGGAGAA TCAGATGGAG GTGCGGAAGA AGGAGATAGA AGAGCTCCAG
    AGCCAGGCGC AGGCCCTGAG CCAGGAGGGG AAGAGCACGG ACGAGGTGGA CAGCAAGCGC
    CTCACCGTGC AGACCAAGTT CATGGAGCTG CTGGAGCCCC TCAACGAGAG GAAGCAGAAC
    CTGCTGGCCT CCAAGGAGAT CCATCAGTTC AACCGGGACG TGGAGGACGA GATCTTGTGG
    GTTGGAGAGA GGATGCCTTT GGCGACCTCC ACGGATCACG GCCACAACCT CCAGACTGTG
    CAGTTGTTAA TAAAGAAAAA TCAGACCCTC CAGAAGGAGA TCCAGGGGCA CCAGCCTCGC
    ATCGATGACA TCTTTGAGAG GAGCCAGAAC ATCATCACTG ACAGCAGCCT CAACGCCGAG
    GCCATCCGGC AGAGACTGGC TGACCTGAAG CAGCTGTGGG GCCTCCTCAT CGAGGAGACG
    GAGAAGCGGC ACCGGCGGCT GGAGGAGGCG CACAGGGCCC AGCAGTACTA CTTCGACGCG
    GCTGAGGCTG AGGCGTGGAT GAGCGAGCAG GAGCTGTACA TGATGTCCGA GGAGAAGGCC
    AAGGATGAGC AGAGTGCCGT CTCCATGTTG AAGAAGCACC AGATTCTGGA ACAAGCTGTG
    GAGGACTATG CGGAGACGGT GCACCAGCTC TCCAAGACCA GCCGGGCCCT GGTGGCCGAC
    AGCCATCCTG AAAGTGAACG TATCAGTATG CGGCAGTCCA AGGTGGACAA GCTGTATGCA
    GGCCTCAAGG ACCTGGCCGA AGAGAGGCGG GGCAAGCTGG ACGAGAGACA CCGGCTGTTC
    CAGCTCAACC GCGAGGTGGA CGACCTGGAG CAGTGGATTG CCGAGAGGGA GGTGGTCGCG
    GGGTCGCATG AGCTGGGCCA GGACTACGAG CACGTCACGA TGCTCCAAGA GCGATTCCGA
    GAGTTCGCCC GGGACACGGG GAACATTGGG CAGGAACGCG TGGACACAGT CAACCACATG
    GCAGACGAGC TCATCAACTC CGGACATTCA GATGCCGCTA CTATTGCTGA GTGGAAGGAT
    GGGCTCAACG AGGCCTGGGC CGACCTCCTG GAGCTCATTG ACACCAGAAC ACAGATCCTC
    GCTGCCTCCT ATGAACTGCA CAAGTTTTAC CACGACGCCA AGGAGATCTT TGGGCGCATC
    CAGGACAAAC ACAAGAAACT CCCCGAGGAG CTCGGGAGAG ATCAGAACAC AGTGGAGACG
    TTACAGAGAA TGCACACCAC CTTTGAGCAC GACATCCAGG CCCTGGGCAC CCAGGTGCGG
    CAGCTGCAGG AAGACGCGGC CCGCCTCCAG GCAGCCTACG CGGGCGACAA GGCCGACGAC
    ATCCAAAAAC GGGAAAACGA GGTGCTGGAA GCCTGGAAGG CCCTGCTAGA CGCCTGCGAA
    GGCCGCAGGG TACGGCTGGT GGACACGGGA GACAAGTTCC GCTTCTTCAG CATGGTGCGC
    GACCTCATGC TGTGGATGGA GGATGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG
    GATGTGTCAT CTGTTGAACT ATTGATGAAT AATCATCAAG GTATCAAAGC TGAAATCGAC
    GCTCGTAATG ACAGTTTTAC GACCTGCATT GAACTGGGGA AATCCCTGCT GGCAAGGAAG
    CACTACGCAT CTGAGGAGAT CAAGGAGAAG TTACTGCAGT TGACAGAAAA GAGAAAAGAA
    ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGAC TGATCTTAGA GGTCCATCAG
    TTCTCAAGGG ATGCCAGTGT GGCCGAGGCC TGGCTGCTCG GGCAGGAGCC CTACCTGTCC
    AGCCGAGAGA TAGGCCAGAG CGTGGACGAG GTTGAGAAGC TCATCAAGCG CCATGAGGCT
    TTTGAAAAGT CTGCAGCGAC CTGGGACGAG AGGTTCTCTG CCCTGGAAAG ACTGACGACG
    TTGGAGCTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGGAAGAG GCGGCCGCCC
    TCCCCCGAGC CGAGCACGAA GGTTTCCGAG GAGACTGAGT CCCAGCAGCA GTGGGATACT
    TCAAAAGGAG AGCAAGTTTC CCAGAATGGT TTGCCGGCTG AACAGGGATC TCCACGGATG
    GCAGAAACAG TGGATACCAG CGAAATGGTT AACGGCGCTG CAGAACAGAG GACGAGCTCG
    AAAGAGTCCA GCCCCATCCC CTCCCCCACC TCTGATCGCA AAGCCAAGAC TGCTCTCCCA
    GCCCAGAGTG CCGCCACCTT GCCAGCCAGG ACCCAGGAGA CACCTTCGGC CCAGATGGAA
    GGCTTCCTGA ACCGGAAGCA CGAGTGGGAG GCCCACAATA AGAAGGCCTC GAGCAGGTCC
    TGGCACAATG TTTATTGTGT CATAAATAAC CAAGAGATGG GTTTCTACAA AGACGCAAAG
    ACAGCTGCTT CAGGAATTCC CTACCACAGC GAGGTCCCTG TGAGTTTGAA AGAAGCCATC
    TGTGAAGTGG CCCTTGATTA CAAGAAGAAG AAGCACGTGT TCAAGCTCAG ACTAAATGAT
    GGCAATGAGT ACCTCTTCCA AGCCAAAGAT GATGAGGAAA TGAACACATG GATCCAGGCC
    ATCTCCTCTG CCATCTCCTC TGATAAACAC GAGGTGTCCG CCAGCACCCA GAGCACGCCA
    GCATCCAGCC GGGCTCAGAC CCTGCCCACC AGCGTCGTCA CCATCACCAG CGAGTCCAGT
    CCCGGCAAAC GGGAGAAGGA CAAAGAGAAA GACAAAGAAA AGAGGTTCAG CCTTTTTGGC
    AAAAAGAAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23932
    Clone ID Related Accession (Same CDS sequence) XM_005212633.3 , XM_005212633.4
    Accession Version XM_005212633.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212633.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCGCTGT CGCCGGCTTA TACGGGGCAG 
    GTGCCTTACA ACTTCAACCA GCTGGAAGGG AGATTCAAGC AGCTCCAAGA TGAACGTGAA
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG GGTGTCCTGC
    CGGATCACAG ATCTGTACAC TGACCTTCGG GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGACT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCACG ACATCGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCTTTGCT CTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AATATTCACA ATTTCACAAC CAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACTTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAGAACG CCTTTAACCT GGCAGAGCAA CACCTTGGCC TCACTAAACT CTTGGACCCC
    GAAGATATCA GTGTGGACCA TCCTGATGAG AAGTCTATTA TCACTTACGT GGTGACTTAC
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAACG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCCCT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACTATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCA
    CTCGTTGGGG TCCAGCAGCA GCTTCAGGCG TTCAACACTT ACCGCACTGT GGAGAAGCCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACCATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATTAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCCCT GCGAAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TTGAGGCCGC CACGAAAAAG CATGAAGCCA TTGAGACTGA CATCGCTGCA
    TACGAGGAGC GCGTGCAGGC TGTGGTGGCT GTGGCCAGGG AACTGGAGGC AGAGAACTAC
    CACGACATCA AGCGCATCAC AGCACGGAAG GACAACGTCA TCCGGCTCTG GGAGTACCTG
    CTGGAGCTGC TCAGGGCCCG GAGACAGCGC CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT CATGGACTGG ATGGATGAAA TGAAGGTGCT GTTATTGTCT
    CAAGACTATG GCAAACACTT GCTCGGCGTG GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAACGCTTC TGCCCAGAAG
    TTTGCAACCG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTAATCCG AGACAGGGTG
    GCCCACATGG AGTTCTGTTA CCAAGAGCTT TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAGGA GGAGGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTACG GCAAGGACCT CACAAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC
    TTCGAGCAAG CCATCAAGGA GGGCGAAGAC ATGATCGCCG AGGAGCACTT CGGGTCAGAG
    AAGATCCGGG AGCGGATTGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCT
    GACGACATCG ATGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC GGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAAG CCAGCGCCCT GCCCCAGGAG
    CACGCCGAGT CCCCCGACGT GCAGGGCCGG CTGGCGGGCA TTGAGGAGCG CTACAAGGAG
    GTGGCCGAGC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCCGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAAGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    CTCGAACCGG AGATGAACAA CCAGGCTTCC CGGGTGGCAG TGGTGAACCA GATTGCCCGG
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGCCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC TCTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCATG GATCCGGGAG
    AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTCGTGG CCATCGAGGC GAAACTGAGT
    GACCTGCAGA AGGAGGCCGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACCAC GCTGAAGAAC
    CGTGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGATGAC
    TTCCAGTCCT GGCTCTCCAG GACACAGACG GCCATTGCCT CGGAGGACAT GCCTAACACC
    CTGACCGAGG CTGAGAAGCT CCTCACGCAG CACGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGCCAGACA
    GATGCCCAGT ACATGTTCCT GCGGCAGCGG CTGCAGGCCC TGGACACAGG CTGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGGCAGAGC CTCCTCTCCC AGTCCCACGC TCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCCGAAGCC TTCCTGAACA ACCAGGAGTA TGTTTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAT
    TTCATGACCA CCATGGATGC CAATGAGGAG AAAATCAACG CGGTTGTGGA AACGGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ACAGGAAGAA TCGCGAGGCA GCCAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAC TGTCAGGAGC TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCCTTTAT GGCAGAACTT GCTTCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAGG GAATGCAGCT CATTTCAGAA AAGCCAGAGA CAGAAGCCGT GGTGAAGGAA
    AAACTCACAG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACCACACA GACCAAGGCC
    CAGCGGCTCT TCGATGCGAA CAAAGCCGAA CTCTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ATGGCCTGGA GAGTCAGATT CAGTCTGACG ACTATGGCAA AGACCTCACC
    AGCGTCAACA TCCTTCTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATAG AAGAGCTCCA GAGCCAGGCG CAGGCCCTGA GCCAGGAGGG GAAGAGCACG
    GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTCAACGAGA GGAAGCAGAA CCTGCTGGCC TCCAAGGAGA TCCATCAGTT CAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACCTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGTTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATCGATGAC ATCTTTGAGA GGAGCCAGAA CATCATCACT
    GACAGCAGCC TCAACGCCGA GGCCATCCGG CAGAGACTGG CTGACCTGAA GCAGCTGTGG
    GGCCTCCTCA TCGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAGGC GCACAGGGCC
    CAGCAGTACT ACTTCGACGC GGCTGAGGCT GAGGCGTGGA TGAGCGAGCA GGAGCTGTAC
    ATGATGTCCG AGGAGAAGGC CAAGGATGAG CAGAGTGCCG TCTCCATGTT GAAGAAGCAC
    CAGATTCTGG AACAAGCTGT GGAGGACTAT GCGGAGACGG TGCACCAGCT CTCCAAGACC
    AGCCGGGCCC TGGTGGCCGA CAGCCATCCT GAAAGTGAAC GTATCAGTAT GCGGCAGTCC
    AAGGTGGACA AGCTGTATGC AGGCCTCAAG GACCTGGCCG AAGAGAGGCG GGGCAAGCTG
    GACGAGAGAC ACCGGCTGTT CCAGCTCAAC CGCGAGGTGG ACGACCTGGA GCAGTGGATT
    GCCGAGAGGG AGGTGGTCGC GGGGTCGCAT GAGCTGGGCC AGGACTACGA GCACGTCACG
    ATGCTCCAAG AGCGATTCCG AGAGTTCGCC CGGGACACGG GGAACATTGG GCAGGAACGC
    GTGGACACAG TCAACCACAT GGCAGACGAG CTCATCAACT CCGGACATTC AGATGCCGCT
    ACTATTGCTG AGTGGAAGGA TGGGCTCAAC GAGGCCTGGG CCGACCTCCT GGAGCTCATT
    GACACCAGAA CACAGATCCT CGCTGCCTCC TATGAACTGC ACAAGTTTTA CCACGACGCC
    AAGGAGATCT TTGGGCGCAT CCAGGACAAA CACAAGAAAC TCCCCGAGGA GCTCGGGAGA
    GATCAGAACA CAGTGGAGAC GTTACAGAGA ATGCACACCA CCTTTGAGCA CGACATCCAG
    GCCCTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGACGCGG CCCGCCTCCA GGCAGCCTAC
    GCGGGCGACA AGGCCGACGA CATCCAAAAA CGGGAAAACG AGGTGCTGGA AGCCTGGAAG
    GCCCTGCTAG ACGCCTGCGA AGGCCGCAGG GTACGGCTGG TGGACACGGG AGACAAGTTC
    CGCTTCTTCA GCATGGTGCG CGACCTCATG CTGTGGATGG AGGATGTCAT CCGGCAGATC
    GAAGCCCAGG AGAAGCCCCG GGATGTGTCA TCTGTTGAAC TATTGATGAA TAATCATCAA
    GGTATCAAAG CTGAAATCGA CGCTCGTAAT GACAGTTTTA CGACCTGCAT TGAACTGGGG
    AAATCCCTGC TGGCAAGGAA GCACTACGCA TCTGAGGAGA TCAAGGAGAA GTTACTGCAG
    TTGACAGAAA AGAGAAAAGA AATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGA
    CTGATCTTAG AGGTCCATCA GTTCTCAAGG GATGCCAGTG TGGCCGAGGC CTGGCTGCTC
    GGGCAGGAGC CCTACCTGTC CAGCCGAGAG ATAGGCCAGA GCGTGGACGA GGTTGAGAAG
    CTCATCAAGC GCCATGAGGC TTTTGAAAAG TCTGCAGCGA CCTGGGACGA GAGGTTCTCT
    GCCCTGGAAA GACTGACGAC GTTGGAGCTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG
    GAGAGGAAGA GGCGGCCGCC CTCCCCCGAG CCGAGCACGA AGGTTTCCGA GGAGACTGAG
    TCCCAGCAGC AGTGGGATAC TTCAAAAGGA GAGCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAACAGGGAT CTCCACGGAT GGCAGAAACA GTGGATACCA GCGAAATGGT TAACGGCGCT
    GCAGAACAGA GGACGAGCTC GAAAGAGTCC AGCCCCATCC CCTCCCCCAC CTCTGATCGC
    AAAGCCAAGA CTGCTCTCCC AGCCCAGAGT GCCGCCACCT TGCCAGCCAG GACCCAGGAG
    ACACCTTCGG CCCAGATGGA AGGCTTCCTG AACCGGAAGC ACGAGTGGGA GGCCCACAAT
    AAGAAGGCCT CGAGCAGGTC CTGGCACAAT GTTTATTGTG TCATAAATAA CCAAGAGATG
    GGTTTCTACA AAGACGCAAA GACAGCTGCT TCAGGAATTC CCTACCACAG CGAGGTCCCT
    GTGAGTTTGA AAGAAGCCAT CTGTGAAGTG GCCCTTGATT ACAAGAAGAA GAAGCACGTG
    TTCAAGCTCA GACTAAATGA TGGCAATGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC CATCTCCTCT GCCATCTCCT CTGATAAACA CGAGGTGTCC
    GCCAGCACCC AGAGCACGCC AGCATCCAGC CGGGCTCAGA CCCTGCCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCCGGCAAA CGGGAGAAGG ACAAAGAGAA AGACAAAGAA
    AAGAGGTTCA GCCTTTTTGG CAAAAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212690.1
    CDS438..7490
    Translation

    Target ORF information:

    RefSeq Version XM_005212633.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212633.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCGCTGT CGCCGGCTTA TACGGGGCAG 
    GTGCCTTACA ACTTCAACCA GCTGGAAGGG AGATTCAAGC AGCTCCAAGA TGAACGTGAA
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG GGTGTCCTGC
    CGGATCACAG ATCTGTACAC TGACCTTCGG GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGACT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCACG ACATCGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCTTTGCT CTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AATATTCACA ATTTCACAAC CAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACTTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAGAACG CCTTTAACCT GGCAGAGCAA CACCTTGGCC TCACTAAACT CTTGGACCCC
    GAAGATATCA GTGTGGACCA TCCTGATGAG AAGTCTATTA TCACTTACGT GGTGACTTAC
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAACG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCCCT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACTATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCA
    CTCGTTGGGG TCCAGCAGCA GCTTCAGGCG TTCAACACTT ACCGCACTGT GGAGAAGCCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACCATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATTAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCCCT GCGAAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TTGAGGCCGC CACGAAAAAG CATGAAGCCA TTGAGACTGA CATCGCTGCA
    TACGAGGAGC GCGTGCAGGC TGTGGTGGCT GTGGCCAGGG AACTGGAGGC AGAGAACTAC
    CACGACATCA AGCGCATCAC AGCACGGAAG GACAACGTCA TCCGGCTCTG GGAGTACCTG
    CTGGAGCTGC TCAGGGCCCG GAGACAGCGC CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT CATGGACTGG ATGGATGAAA TGAAGGTGCT GTTATTGTCT
    CAAGACTATG GCAAACACTT GCTCGGCGTG GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAACGCTTC TGCCCAGAAG
    TTTGCAACCG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTAATCCG AGACAGGGTG
    GCCCACATGG AGTTCTGTTA CCAAGAGCTT TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAGGA GGAGGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTACG GCAAGGACCT CACAAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC
    TTCGAGCAAG CCATCAAGGA GGGCGAAGAC ATGATCGCCG AGGAGCACTT CGGGTCAGAG
    AAGATCCGGG AGCGGATTGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCT
    GACGACATCG ATGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC GGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAAG CCAGCGCCCT GCCCCAGGAG
    CACGCCGAGT CCCCCGACGT GCAGGGCCGG CTGGCGGGCA TTGAGGAGCG CTACAAGGAG
    GTGGCCGAGC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCCGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAAGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    CTCGAACCGG AGATGAACAA CCAGGCTTCC CGGGTGGCAG TGGTGAACCA GATTGCCCGG
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGCCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC TCTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCATG GATCCGGGAG
    AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTCGTGG CCATCGAGGC GAAACTGAGT
    GACCTGCAGA AGGAGGCCGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACCAC GCTGAAGAAC
    CGTGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGATGAC
    TTCCAGTCCT GGCTCTCCAG GACACAGACG GCCATTGCCT CGGAGGACAT GCCTAACACC
    CTGACCGAGG CTGAGAAGCT CCTCACGCAG CACGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGCCAGACA
    GATGCCCAGT ACATGTTCCT GCGGCAGCGG CTGCAGGCCC TGGACACAGG CTGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGGCAGAGC CTCCTCTCCC AGTCCCACGC TCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCCGAAGCC TTCCTGAACA ACCAGGAGTA TGTTTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAT
    TTCATGACCA CCATGGATGC CAATGAGGAG AAAATCAACG CGGTTGTGGA AACGGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ACAGGAAGAA TCGCGAGGCA GCCAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAC TGTCAGGAGC TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCCTTTAT GGCAGAACTT GCTTCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAGG GAATGCAGCT CATTTCAGAA AAGCCAGAGA CAGAAGCCGT GGTGAAGGAA
    AAACTCACAG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACCACACA GACCAAGGCC
    CAGCGGCTCT TCGATGCGAA CAAAGCCGAA CTCTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ATGGCCTGGA GAGTCAGATT CAGTCTGACG ACTATGGCAA AGACCTCACC
    AGCGTCAACA TCCTTCTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATAG AAGAGCTCCA GAGCCAGGCG CAGGCCCTGA GCCAGGAGGG GAAGAGCACG
    GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTCAACGAGA GGAAGCAGAA CCTGCTGGCC TCCAAGGAGA TCCATCAGTT CAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACCTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGTTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATCGATGAC ATCTTTGAGA GGAGCCAGAA CATCATCACT
    GACAGCAGCC TCAACGCCGA GGCCATCCGG CAGAGACTGG CTGACCTGAA GCAGCTGTGG
    GGCCTCCTCA TCGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAGGC GCACAGGGCC
    CAGCAGTACT ACTTCGACGC GGCTGAGGCT GAGGCGTGGA TGAGCGAGCA GGAGCTGTAC
    ATGATGTCCG AGGAGAAGGC CAAGGATGAG CAGAGTGCCG TCTCCATGTT GAAGAAGCAC
    CAGATTCTGG AACAAGCTGT GGAGGACTAT GCGGAGACGG TGCACCAGCT CTCCAAGACC
    AGCCGGGCCC TGGTGGCCGA CAGCCATCCT GAAAGTGAAC GTATCAGTAT GCGGCAGTCC
    AAGGTGGACA AGCTGTATGC AGGCCTCAAG GACCTGGCCG AAGAGAGGCG GGGCAAGCTG
    GACGAGAGAC ACCGGCTGTT CCAGCTCAAC CGCGAGGTGG ACGACCTGGA GCAGTGGATT
    GCCGAGAGGG AGGTGGTCGC GGGGTCGCAT GAGCTGGGCC AGGACTACGA GCACGTCACG
    ATGCTCCAAG AGCGATTCCG AGAGTTCGCC CGGGACACGG GGAACATTGG GCAGGAACGC
    GTGGACACAG TCAACCACAT GGCAGACGAG CTCATCAACT CCGGACATTC AGATGCCGCT
    ACTATTGCTG AGTGGAAGGA TGGGCTCAAC GAGGCCTGGG CCGACCTCCT GGAGCTCATT
    GACACCAGAA CACAGATCCT CGCTGCCTCC TATGAACTGC ACAAGTTTTA CCACGACGCC
    AAGGAGATCT TTGGGCGCAT CCAGGACAAA CACAAGAAAC TCCCCGAGGA GCTCGGGAGA
    GATCAGAACA CAGTGGAGAC GTTACAGAGA ATGCACACCA CCTTTGAGCA CGACATCCAG
    GCCCTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGACGCGG CCCGCCTCCA GGCAGCCTAC
    GCGGGCGACA AGGCCGACGA CATCCAAAAA CGGGAAAACG AGGTGCTGGA AGCCTGGAAG
    GCCCTGCTAG ACGCCTGCGA AGGCCGCAGG GTACGGCTGG TGGACACGGG AGACAAGTTC
    CGCTTCTTCA GCATGGTGCG CGACCTCATG CTGTGGATGG AGGATGTCAT CCGGCAGATC
    GAAGCCCAGG AGAAGCCCCG GGATGTGTCA TCTGTTGAAC TATTGATGAA TAATCATCAA
    GGTATCAAAG CTGAAATCGA CGCTCGTAAT GACAGTTTTA CGACCTGCAT TGAACTGGGG
    AAATCCCTGC TGGCAAGGAA GCACTACGCA TCTGAGGAGA TCAAGGAGAA GTTACTGCAG
    TTGACAGAAA AGAGAAAAGA AATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGA
    CTGATCTTAG AGGTCCATCA GTTCTCAAGG GATGCCAGTG TGGCCGAGGC CTGGCTGCTC
    GGGCAGGAGC CCTACCTGTC CAGCCGAGAG ATAGGCCAGA GCGTGGACGA GGTTGAGAAG
    CTCATCAAGC GCCATGAGGC TTTTGAAAAG TCTGCAGCGA CCTGGGACGA GAGGTTCTCT
    GCCCTGGAAA GACTGACGAC GTTGGAGCTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG
    GAGAGGAAGA GGCGGCCGCC CTCCCCCGAG CCGAGCACGA AGGTTTCCGA GGAGACTGAG
    TCCCAGCAGC AGTGGGATAC TTCAAAAGGA GAGCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAACAGGGAT CTCCACGGAT GGCAGAAACA GTGGATACCA GCGAAATGGT TAACGGCGCT
    GCAGAACAGA GGACGAGCTC GAAAGAGTCC AGCCCCATCC CCTCCCCCAC CTCTGATCGC
    AAAGCCAAGA CTGCTCTCCC AGCCCAGAGT GCCGCCACCT TGCCAGCCAG GACCCAGGAG
    ACACCTTCGG CCCAGATGGA AGGCTTCCTG AACCGGAAGC ACGAGTGGGA GGCCCACAAT
    AAGAAGGCCT CGAGCAGGTC CTGGCACAAT GTTTATTGTG TCATAAATAA CCAAGAGATG
    GGTTTCTACA AAGACGCAAA GACAGCTGCT TCAGGAATTC CCTACCACAG CGAGGTCCCT
    GTGAGTTTGA AAGAAGCCAT CTGTGAAGTG GCCCTTGATT ACAAGAAGAA GAAGCACGTG
    TTCAAGCTCA GACTAAATGA TGGCAATGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC CATCTCCTCT GCCATCTCCT CTGATAAACA CGAGGTGTCC
    GCCAGCACCC AGAGCACGCC AGCATCCAGC CGGGCTCAGA CCCTGCCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCCGGCAAA CGGGAGAAGG ACAAAGAGAA AGACAAAGAA
    AAGAGGTTCA GCCTTTTTGG CAAAAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23932
    Clone ID Related Accession (Same CDS sequence) XM_005212633.3 , XM_005212633.4
    Accession Version XM_005212633.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7053bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005212633.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCGCTGT CGCCGGCTTA TACGGGGCAG 
    GTGCCTTACA ACTTCAACCA GCTGGAAGGG AGATTCAAGC AGCTCCAAGA TGAACGTGAA
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG GGTGTCCTGC
    CGGATCACAG ATCTGTACAC TGACCTTCGG GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGACT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCACG ACATCGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCTTTGCT CTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AATATTCACA ATTTCACAAC CAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACTTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAGAACG CCTTTAACCT GGCAGAGCAA CACCTTGGCC TCACTAAACT CTTGGACCCC
    GAAGATATCA GTGTGGACCA TCCTGATGAG AAGTCTATTA TCACTTACGT GGTGACTTAC
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAACG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCCCT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACTATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCA
    CTCGTTGGGG TCCAGCAGCA GCTTCAGGCG TTCAACACTT ACCGCACTGT GGAGAAGCCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACCATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATTAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCCCT GCGAAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TTGAGGCCGC CACGAAAAAG CATGAAGCCA TTGAGACTGA CATCGCTGCA
    TACGAGGAGC GCGTGCAGGC TGTGGTGGCT GTGGCCAGGG AACTGGAGGC AGAGAACTAC
    CACGACATCA AGCGCATCAC AGCACGGAAG GACAACGTCA TCCGGCTCTG GGAGTACCTG
    CTGGAGCTGC TCAGGGCCCG GAGACAGCGC CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT CATGGACTGG ATGGATGAAA TGAAGGTGCT GTTATTGTCT
    CAAGACTATG GCAAACACTT GCTCGGCGTG GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAACGCTTC TGCCCAGAAG
    TTTGCAACCG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTAATCCG AGACAGGGTG
    GCCCACATGG AGTTCTGTTA CCAAGAGCTT TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAGGA GGAGGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTACG GCAAGGACCT CACAAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC
    TTCGAGCAAG CCATCAAGGA GGGCGAAGAC ATGATCGCCG AGGAGCACTT CGGGTCAGAG
    AAGATCCGGG AGCGGATTGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCT
    GACGACATCG ATGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC GGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAAG CCAGCGCCCT GCCCCAGGAG
    CACGCCGAGT CCCCCGACGT GCAGGGCCGG CTGGCGGGCA TTGAGGAGCG CTACAAGGAG
    GTGGCCGAGC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCCGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAAGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    CTCGAACCGG AGATGAACAA CCAGGCTTCC CGGGTGGCAG TGGTGAACCA GATTGCCCGG
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGCCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC TCTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCATG GATCCGGGAG
    AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTCGTGG CCATCGAGGC GAAACTGAGT
    GACCTGCAGA AGGAGGCCGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACCAC GCTGAAGAAC
    CGTGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGATGAC
    TTCCAGTCCT GGCTCTCCAG GACACAGACG GCCATTGCCT CGGAGGACAT GCCTAACACC
    CTGACCGAGG CTGAGAAGCT CCTCACGCAG CACGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGCCAGACA
    GATGCCCAGT ACATGTTCCT GCGGCAGCGG CTGCAGGCCC TGGACACAGG CTGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGGCAGAGC CTCCTCTCCC AGTCCCACGC TCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCCGAAGCC TTCCTGAACA ACCAGGAGTA TGTTTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAT
    TTCATGACCA CCATGGATGC CAATGAGGAG AAAATCAACG CGGTTGTGGA AACGGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ACAGGAAGAA TCGCGAGGCA GCCAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAC TGTCAGGAGC TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCCTTTAT GGCAGAACTT GCTTCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAGG GAATGCAGCT CATTTCAGAA AAGCCAGAGA CAGAAGCCGT GGTGAAGGAA
    AAACTCACAG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACCACACA GACCAAGGCC
    CAGCGGCTCT TCGATGCGAA CAAAGCCGAA CTCTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ATGGCCTGGA GAGTCAGATT CAGTCTGACG ACTATGGCAA AGACCTCACC
    AGCGTCAACA TCCTTCTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATAG AAGAGCTCCA GAGCCAGGCG CAGGCCCTGA GCCAGGAGGG GAAGAGCACG
    GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTCAACGAGA GGAAGCAGAA CCTGCTGGCC TCCAAGGAGA TCCATCAGTT CAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACCTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGTTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATCGATGAC ATCTTTGAGA GGAGCCAGAA CATCATCACT
    GACAGCAGCC TCAACGCCGA GGCCATCCGG CAGAGACTGG CTGACCTGAA GCAGCTGTGG
    GGCCTCCTCA TCGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAGGC GCACAGGGCC
    CAGCAGTACT ACTTCGACGC GGCTGAGGCT GAGGCGTGGA TGAGCGAGCA GGAGCTGTAC
    ATGATGTCCG AGGAGAAGGC CAAGGATGAG CAGAGTGCCG TCTCCATGTT GAAGAAGCAC
    CAGATTCTGG AACAAGCTGT GGAGGACTAT GCGGAGACGG TGCACCAGCT CTCCAAGACC
    AGCCGGGCCC TGGTGGCCGA CAGCCATCCT GAAAGTGAAC GTATCAGTAT GCGGCAGTCC
    AAGGTGGACA AGCTGTATGC AGGCCTCAAG GACCTGGCCG AAGAGAGGCG GGGCAAGCTG
    GACGAGAGAC ACCGGCTGTT CCAGCTCAAC CGCGAGGTGG ACGACCTGGA GCAGTGGATT
    GCCGAGAGGG AGGTGGTCGC GGGGTCGCAT GAGCTGGGCC AGGACTACGA GCACGTCACG
    ATGCTCCAAG AGCGATTCCG AGAGTTCGCC CGGGACACGG GGAACATTGG GCAGGAACGC
    GTGGACACAG TCAACCACAT GGCAGACGAG CTCATCAACT CCGGACATTC AGATGCCGCT
    ACTATTGCTG AGTGGAAGGA TGGGCTCAAC GAGGCCTGGG CCGACCTCCT GGAGCTCATT
    GACACCAGAA CACAGATCCT CGCTGCCTCC TATGAACTGC ACAAGTTTTA CCACGACGCC
    AAGGAGATCT TTGGGCGCAT CCAGGACAAA CACAAGAAAC TCCCCGAGGA GCTCGGGAGA
    GATCAGAACA CAGTGGAGAC GTTACAGAGA ATGCACACCA CCTTTGAGCA CGACATCCAG
    GCCCTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGACGCGG CCCGCCTCCA GGCAGCCTAC
    GCGGGCGACA AGGCCGACGA CATCCAAAAA CGGGAAAACG AGGTGCTGGA AGCCTGGAAG
    GCCCTGCTAG ACGCCTGCGA AGGCCGCAGG GTACGGCTGG TGGACACGGG AGACAAGTTC
    CGCTTCTTCA GCATGGTGCG CGACCTCATG CTGTGGATGG AGGATGTCAT CCGGCAGATC
    GAAGCCCAGG AGAAGCCCCG GGATGTGTCA TCTGTTGAAC TATTGATGAA TAATCATCAA
    GGTATCAAAG CTGAAATCGA CGCTCGTAAT GACAGTTTTA CGACCTGCAT TGAACTGGGG
    AAATCCCTGC TGGCAAGGAA GCACTACGCA TCTGAGGAGA TCAAGGAGAA GTTACTGCAG
    TTGACAGAAA AGAGAAAAGA AATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGA
    CTGATCTTAG AGGTCCATCA GTTCTCAAGG GATGCCAGTG TGGCCGAGGC CTGGCTGCTC
    GGGCAGGAGC CCTACCTGTC CAGCCGAGAG ATAGGCCAGA GCGTGGACGA GGTTGAGAAG
    CTCATCAAGC GCCATGAGGC TTTTGAAAAG TCTGCAGCGA CCTGGGACGA GAGGTTCTCT
    GCCCTGGAAA GACTGACGAC GTTGGAGCTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG
    GAGAGGAAGA GGCGGCCGCC CTCCCCCGAG CCGAGCACGA AGGTTTCCGA GGAGACTGAG
    TCCCAGCAGC AGTGGGATAC TTCAAAAGGA GAGCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAACAGGGAT CTCCACGGAT GGCAGAAACA GTGGATACCA GCGAAATGGT TAACGGCGCT
    GCAGAACAGA GGACGAGCTC GAAAGAGTCC AGCCCCATCC CCTCCCCCAC CTCTGATCGC
    AAAGCCAAGA CTGCTCTCCC AGCCCAGAGT GCCGCCACCT TGCCAGCCAG GACCCAGGAG
    ACACCTTCGG CCCAGATGGA AGGCTTCCTG AACCGGAAGC ACGAGTGGGA GGCCCACAAT
    AAGAAGGCCT CGAGCAGGTC CTGGCACAAT GTTTATTGTG TCATAAATAA CCAAGAGATG
    GGTTTCTACA AAGACGCAAA GACAGCTGCT TCAGGAATTC CCTACCACAG CGAGGTCCCT
    GTGAGTTTGA AAGAAGCCAT CTGTGAAGTG GCCCTTGATT ACAAGAAGAA GAAGCACGTG
    TTCAAGCTCA GACTAAATGA TGGCAATGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC CATCTCCTCT GCCATCTCCT CTGATAAACA CGAGGTGTCC
    GCCAGCACCC AGAGCACGCC AGCATCCAGC CGGGCTCAGA CCCTGCCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCCGGCAAA CGGGAGAAGG ACAAAGAGAA AGACAAAGAA
    AAGAGGTTCA GCCTTTTTGG CAAAAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212690.1
    CDS1056..8108
    Translation

    Target ORF information:

    RefSeq Version XM_005212633.4
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212633.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCGCTGT CGCCGGCTTA TACGGGGCAG 
    GTGCCTTACA ACTTCAACCA GCTGGAAGGG AGATTCAAGC AGCTCCAAGA TGAACGTGAA
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG GGTGTCCTGC
    CGGATCACAG ATCTGTACAC TGACCTTCGG GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGACT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCACG ACATCGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCTTTGCT CTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AATATTCACA ATTTCACAAC CAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACTTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAGAACG CCTTTAACCT GGCAGAGCAA CACCTTGGCC TCACTAAACT CTTGGACCCC
    GAAGATATCA GTGTGGACCA TCCTGATGAG AAGTCTATTA TCACTTACGT GGTGACTTAC
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAACG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCCCT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACTATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCA
    CTCGTTGGGG TCCAGCAGCA GCTTCAGGCG TTCAACACTT ACCGCACTGT GGAGAAGCCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACCATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATTAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCCCT GCGAAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TTGAGGCCGC CACGAAAAAG CATGAAGCCA TTGAGACTGA CATCGCTGCA
    TACGAGGAGC GCGTGCAGGC TGTGGTGGCT GTGGCCAGGG AACTGGAGGC AGAGAACTAC
    CACGACATCA AGCGCATCAC AGCACGGAAG GACAACGTCA TCCGGCTCTG GGAGTACCTG
    CTGGAGCTGC TCAGGGCCCG GAGACAGCGC CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT CATGGACTGG ATGGATGAAA TGAAGGTGCT GTTATTGTCT
    CAAGACTATG GCAAACACTT GCTCGGCGTG GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAACGCTTC TGCCCAGAAG
    TTTGCAACCG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTAATCCG AGACAGGGTG
    GCCCACATGG AGTTCTGTTA CCAAGAGCTT TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAGGA GGAGGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTACG GCAAGGACCT CACAAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC
    TTCGAGCAAG CCATCAAGGA GGGCGAAGAC ATGATCGCCG AGGAGCACTT CGGGTCAGAG
    AAGATCCGGG AGCGGATTGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCT
    GACGACATCG ATGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC GGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAAG CCAGCGCCCT GCCCCAGGAG
    CACGCCGAGT CCCCCGACGT GCAGGGCCGG CTGGCGGGCA TTGAGGAGCG CTACAAGGAG
    GTGGCCGAGC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCCGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAAGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    CTCGAACCGG AGATGAACAA CCAGGCTTCC CGGGTGGCAG TGGTGAACCA GATTGCCCGG
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGCCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC TCTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCATG GATCCGGGAG
    AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTCGTGG CCATCGAGGC GAAACTGAGT
    GACCTGCAGA AGGAGGCCGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACCAC GCTGAAGAAC
    CGTGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGATGAC
    TTCCAGTCCT GGCTCTCCAG GACACAGACG GCCATTGCCT CGGAGGACAT GCCTAACACC
    CTGACCGAGG CTGAGAAGCT CCTCACGCAG CACGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGCCAGACA
    GATGCCCAGT ACATGTTCCT GCGGCAGCGG CTGCAGGCCC TGGACACAGG CTGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGGCAGAGC CTCCTCTCCC AGTCCCACGC TCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCCGAAGCC TTCCTGAACA ACCAGGAGTA TGTTTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAT
    TTCATGACCA CCATGGATGC CAATGAGGAG AAAATCAACG CGGTTGTGGA AACGGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ACAGGAAGAA TCGCGAGGCA GCCAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAC TGTCAGGAGC TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCCTTTAT GGCAGAACTT GCTTCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAGG GAATGCAGCT CATTTCAGAA AAGCCAGAGA CAGAAGCCGT GGTGAAGGAA
    AAACTCACAG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACCACACA GACCAAGGCC
    CAGCGGCTCT TCGATGCGAA CAAAGCCGAA CTCTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ATGGCCTGGA GAGTCAGATT CAGTCTGACG ACTATGGCAA AGACCTCACC
    AGCGTCAACA TCCTTCTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATAG AAGAGCTCCA GAGCCAGGCG CAGGCCCTGA GCCAGGAGGG GAAGAGCACG
    GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTCAACGAGA GGAAGCAGAA CCTGCTGGCC TCCAAGGAGA TCCATCAGTT CAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACCTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGTTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATCGATGAC ATCTTTGAGA GGAGCCAGAA CATCATCACT
    GACAGCAGCC TCAACGCCGA GGCCATCCGG CAGAGACTGG CTGACCTGAA GCAGCTGTGG
    GGCCTCCTCA TCGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAGGC GCACAGGGCC
    CAGCAGTACT ACTTCGACGC GGCTGAGGCT GAGGCGTGGA TGAGCGAGCA GGAGCTGTAC
    ATGATGTCCG AGGAGAAGGC CAAGGATGAG CAGAGTGCCG TCTCCATGTT GAAGAAGCAC
    CAGATTCTGG AACAAGCTGT GGAGGACTAT GCGGAGACGG TGCACCAGCT CTCCAAGACC
    AGCCGGGCCC TGGTGGCCGA CAGCCATCCT GAAAGTGAAC GTATCAGTAT GCGGCAGTCC
    AAGGTGGACA AGCTGTATGC AGGCCTCAAG GACCTGGCCG AAGAGAGGCG GGGCAAGCTG
    GACGAGAGAC ACCGGCTGTT CCAGCTCAAC CGCGAGGTGG ACGACCTGGA GCAGTGGATT
    GCCGAGAGGG AGGTGGTCGC GGGGTCGCAT GAGCTGGGCC AGGACTACGA GCACGTCACG
    ATGCTCCAAG AGCGATTCCG AGAGTTCGCC CGGGACACGG GGAACATTGG GCAGGAACGC
    GTGGACACAG TCAACCACAT GGCAGACGAG CTCATCAACT CCGGACATTC AGATGCCGCT
    ACTATTGCTG AGTGGAAGGA TGGGCTCAAC GAGGCCTGGG CCGACCTCCT GGAGCTCATT
    GACACCAGAA CACAGATCCT CGCTGCCTCC TATGAACTGC ACAAGTTTTA CCACGACGCC
    AAGGAGATCT TTGGGCGCAT CCAGGACAAA CACAAGAAAC TCCCCGAGGA GCTCGGGAGA
    GATCAGAACA CAGTGGAGAC GTTACAGAGA ATGCACACCA CCTTTGAGCA CGACATCCAG
    GCCCTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGACGCGG CCCGCCTCCA GGCAGCCTAC
    GCGGGCGACA AGGCCGACGA CATCCAAAAA CGGGAAAACG AGGTGCTGGA AGCCTGGAAG
    GCCCTGCTAG ACGCCTGCGA AGGCCGCAGG GTACGGCTGG TGGACACGGG AGACAAGTTC
    CGCTTCTTCA GCATGGTGCG CGACCTCATG CTGTGGATGG AGGATGTCAT CCGGCAGATC
    GAAGCCCAGG AGAAGCCCCG GGATGTGTCA TCTGTTGAAC TATTGATGAA TAATCATCAA
    GGTATCAAAG CTGAAATCGA CGCTCGTAAT GACAGTTTTA CGACCTGCAT TGAACTGGGG
    AAATCCCTGC TGGCAAGGAA GCACTACGCA TCTGAGGAGA TCAAGGAGAA GTTACTGCAG
    TTGACAGAAA AGAGAAAAGA AATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGA
    CTGATCTTAG AGGTCCATCA GTTCTCAAGG GATGCCAGTG TGGCCGAGGC CTGGCTGCTC
    GGGCAGGAGC CCTACCTGTC CAGCCGAGAG ATAGGCCAGA GCGTGGACGA GGTTGAGAAG
    CTCATCAAGC GCCATGAGGC TTTTGAAAAG TCTGCAGCGA CCTGGGACGA GAGGTTCTCT
    GCCCTGGAAA GACTGACGAC GTTGGAGCTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG
    GAGAGGAAGA GGCGGCCGCC CTCCCCCGAG CCGAGCACGA AGGTTTCCGA GGAGACTGAG
    TCCCAGCAGC AGTGGGATAC TTCAAAAGGA GAGCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAACAGGGAT CTCCACGGAT GGCAGAAACA GTGGATACCA GCGAAATGGT TAACGGCGCT
    GCAGAACAGA GGACGAGCTC GAAAGAGTCC AGCCCCATCC CCTCCCCCAC CTCTGATCGC
    AAAGCCAAGA CTGCTCTCCC AGCCCAGAGT GCCGCCACCT TGCCAGCCAG GACCCAGGAG
    ACACCTTCGG CCCAGATGGA AGGCTTCCTG AACCGGAAGC ACGAGTGGGA GGCCCACAAT
    AAGAAGGCCT CGAGCAGGTC CTGGCACAAT GTTTATTGTG TCATAAATAA CCAAGAGATG
    GGTTTCTACA AAGACGCAAA GACAGCTGCT TCAGGAATTC CCTACCACAG CGAGGTCCCT
    GTGAGTTTGA AAGAAGCCAT CTGTGAAGTG GCCCTTGATT ACAAGAAGAA GAAGCACGTG
    TTCAAGCTCA GACTAAATGA TGGCAATGAG TACCTCTTCC AAGCCAAAGA TGATGAGGAA
    ATGAACACAT GGATCCAGGC CATCTCCTCT GCCATCTCCT CTGATAAACA CGAGGTGTCC
    GCCAGCACCC AGAGCACGCC AGCATCCAGC CGGGCTCAGA CCCTGCCCAC CAGCGTCGTC
    ACCATCACCA GCGAGTCCAG TCCCGGCAAA CGGGAGAAGG ACAAAGAGAA AGACAAAGAA
    AAGAGGTTCA GCCTTTTTGG CAAAAAGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23933
    Clone ID Related Accession (Same CDS sequence) XM_005212634.3
    Accession Version XM_005212634.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product spectrin beta chain, non-erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104293.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005212634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCGCTGT CGCCGGCTTA TACGGGGCAG 
    GTGCCTTACA ACTTCAACCA GCTGGAAGGG AGATTCAAGC AGCTCCAAGA TGAACGTGAA
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG GGTGTCCTGC
    CGGATCACAG ATCTGTACAC TGACCTTCGG GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGACT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCACG ACATCGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCTTTGCT CTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AATATTCACA ATTTCACAAC CAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACTTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAGAACG CCTTTAACCT GGCAGAGCAA CACCTTGGCC TCACTAAACT CTTGGACCCC
    GAAGATATCA GTGTGGACCA TCCTGATGAG AAGTCTATTA TCACTTACGT GGTGACTTAC
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAACG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCCCT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACTATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCA
    CTCGTTGGGG TCCAGCAGCA GCTTCAGGCG TTCAACACTT ACCGCACTGT GGAGAAGCCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACCATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATTAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCCCT GCGAAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TTGAGGCCGC CACGAAAAAG CATGAAGCCA TTGAGACTGA CATCGCTGCA
    TACGAGGAGC GCGTGCAGGC TGTGGTGGCT GTGGCCAGGG AACTGGAGGC AGAGAACTAC
    CACGACATCA AGCGCATCAC AGCACGGAAG GACAACGTCA TCCGGCTCTG GGAGTACCTG
    CTGGAGCTGC TCAGGGCCCG GAGACAGCGC CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT CATGGACTGG ATGGATGAAA TGAAGGTGCT GTTATTGTCT
    CAAGACTATG GCAAACACTT GCTCGGCGTG GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAACGCTTC TGCCCAGAAG
    TTTGCAACCG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTAATCCG AGACAGGGTG
    GCCCACATGG AGTTCTGTTA CCAAGAGCTT TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAGGA GGAGGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTACG GCAAGGACCT CACAAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC
    TTCGAGCAAG CCATCAAGGA GGGCGAAGAC ATGATCGCCG AGGAGCACTT CGGGTCAGAG
    AAGATCCGGG AGCGGATTGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCT
    GACGACATCG ATGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC GGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAAG CCAGCGCCCT GCCCCAGGAG
    CACGCCGAGT CCCCCGACGT GCAGGGCCGG CTGGCGGGCA TTGAGGAGCG CTACAAGGAG
    GTGGCCGAGC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCCGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAAGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    CTCGAACCGG AGATGAACAA CCAGGCTTCC CGGGTGGCAG TGGTGAACCA GATTGCCCGG
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGCCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC TCTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCATG GATCCGGGAG
    AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTCGTGG CCATCGAGGC GAAACTGAGT
    GACCTGCAGA AGGAGGCCGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACCAC GCTGAAGAAC
    CGTGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGATGAC
    TTCCAGTCCT GGCTCTCCAG GACACAGACG GCCATTGCCT CGGAGGACAT GCCTAACACC
    CTGACCGAGG CTGAGAAGCT CCTCACGCAG CACGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGCCAGACA
    GATGCCCAGT ACATGTTCCT GCGGCAGCGG CTGCAGGCCC TGGACACAGG CTGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGGCAGAGC CTCCTCTCCC AGTCCCACGC TCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCCGAAGCC TTCCTGAACA ACCAGGAGTA TGTTTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAT
    TTCATGACCA CCATGGATGC CAATGAGGAG AAAATCAACG CGGTTGTGGA AACGGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ACAGGAAGAA TCGCGAGGCA GCCAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAC TGTCAGGAGC TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCCTTTAT GGCAGAACTT GCTTCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAGG GAATGCAGCT CATTTCAGAA AAGCCAGAGA CAGAAGCCGT GGTGAAGGAA
    AAACTCACAG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACCACACA GACCAAGGCC
    CAGCGGCTCT TCGATGCGAA CAAAGCCGAA CTCTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ATGGCCTGGA GAGTCAGATT CAGTCTGACG ACTATGGCAA AGACCTCACC
    AGCGTCAACA TCCTTCTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATAG AAGAGCTCCA GAGCCAGGCG CAGGCCCTGA GCCAGGAGGG GAAGAGCACG
    GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTCAACGAGA GGAAGCAGAA CCTGCTGGCC TCCAAGGAGA TCCATCAGTT CAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACCTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGTTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATCGATGAC ATCTTTGAGA GGAGCCAGAA CATCATCACT
    GACAGCAGCC TCAACGCCGA GGCCATCCGG CAGAGACTGG CTGACCTGAA GCAGCTGTGG
    GGCCTCCTCA TCGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAGGC GCACAGGGCC
    CAGCAGTACT ACTTCGACGC GGCTGAGGCT GAGGCGTGGA TGAGCGAGCA GGAGCTGTAC
    ATGATGTCCG AGGAGAAGGC CAAGGATGAG CAGAGTGCCG TCTCCATGTT GAAGAAGCAC
    CAGATTCTGG AACAAGCTGT GGAGGACTAT GCGGAGACGG TGCACCAGCT CTCCAAGACC
    AGCCGGGCCC TGGTGGCCGA CAGCCATCCT GAAAGTGAAC GTATCAGTAT GCGGCAGTCC
    AAGGTGGACA AGCTGTATGC AGGCCTCAAG GACCTGGCCG AAGAGAGGCG GGGCAAGCTG
    GACGAGAGAC ACCGGCTGTT CCAGCTCAAC CGCGAGGTGG ACGACCTGGA GCAGTGGATT
    GCCGAGAGGG AGGTGGTCGC GGGGTCGCAT GAGCTGGGCC AGGACTACGA GCACGTCACG
    ATGCTCCAAG AGCGATTCCG AGAGTTCGCC CGGGACACGG GGAACATTGG GCAGGAACGC
    GTGGACACAG TCAACCACAT GGCAGACGAG CTCATCAACT CCGGACATTC AGATGCCGCT
    ACTATTGCTG AGTGGAAGGA TGGGCTCAAC GAGGCCTGGG CCGACCTCCT GGAGCTCATT
    GACACCAGAA CACAGATCCT CGCTGCCTCC TATGAACTGC ACAAGTTTTA CCACGACGCC
    AAGGAGATCT TTGGGCGCAT CCAGGACAAA CACAAGAAAC TCCCCGAGGA GCTCGGGAGA
    GATCAGAACA CAGTGGAGAC GTTACAGAGA ATGCACACCA CCTTTGAGCA CGACATCCAG
    GCCCTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGACGCGG CCCGCCTCCA GGCAGCCTAC
    GCGGGCGACA AGGCCGACGA CATCCAAAAA CGGGAAAACG AGGTGCTGGA AGCCTGGAAG
    GCCCTGCTAG ACGCCTGCGA AGGCCGCAGG GTACGGCTGG TGGACACGGG AGACAAGTTC
    CGCTTCTTCA GCATGGTGCG CGACCTCATG CTGTGGATGG AGGATGTCAT CCGGCAGATC
    GAAGCCCAGG AGAAGCCCCG GGATGTGTCA TCTGTTGAAC TATTGATGAA TAATCATCAA
    GGTATCAAAG CTGAAATCGA CGCTCGTAAT GACAGTTTTA CGACCTGCAT TGAACTGGGG
    AAATCCCTGC TGGCAAGGAA GCACTACGCA TCTGAGGAGA TCAAGGAGAA GTTACTGCAG
    TTGACAGAAA AGAGAAAAGA AATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGA
    CTGATCTTAG AGGTCCATCA GTTCTCAAGG GATGCCAGTG TGGCCGAGGC CTGGCTGCTC
    GGGCAGGAGC CCTACCTGTC CAGCCGAGAG ATAGGCCAGA GCGTGGACGA GGTTGAGAAG
    CTCATCAAGC GCCATGAGGC TTTTGAAAAG TCTGCAGCGA CCTGGGACGA GAGGTTCTCT
    GCCCTGGAAA GACTGACGAC GTTGGAGCTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG
    GAGAGGAAGA GGCGGCCGCC CTCCCCCGAG CCGAGCACGA AGGTTTCCGA GGAGACTGAG
    TCCCAGCAGC AGTGGGATAC TTCAAAAGGA GAGCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAACAGGGAT CTCCACGGGT TAGTTACCGC TCTCAAACCT ACCAAAACTA CAAAAACTTT
    AATAGCAGAC GGACAGCCAG TGACCAGCCG TGGTCTGGAC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005212691.1
    CDS1..6465
    Translation

    Target ORF information:

    RefSeq Version XM_005212634.3
    Organism Bos taurus(cattle)
    Definition Bos taurus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005212634.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGAATTGC AGAGGACGTC TAGCATCTCC GGGCCGCTGT CGCCGGCTTA TACGGGGCAG 
    GTGCCTTACA ACTTCAACCA GCTGGAAGGG AGATTCAAGC AGCTCCAAGA TGAACGTGAA
    GCTGTGCAAA AGAAGACCTT CACCAAGTGG GTCAACTCCC ACCTTGCCCG GGTGTCCTGC
    CGGATCACAG ATCTGTACAC TGACCTTCGG GATGGACGGA TGCTCATCAA GCTACTGGAG
    GTCCTCTCTG GAGAGAGACT GCCTAAACCC ACCAAGGGAC GAATGCGCAT CCACTGTCTG
    GAGAATGTGG ACAAGGCCCT TCAGTTCCTG AAGGAGCAGA GAGTCCATCT TGAGAACATG
    GGGTCCCACG ACATCGTGGA TGGAAACCAC CGGCTGACCC TCGGCCTCAT CTGGACCATC
    ATCCTGCGCT TCCAGATCCA GGATATCAGT GTAGAAACTG AAGACAACAA AGAGAAGAAA
    TCTGCCAAGG ATGCTTTGCT CTTGTGGTGC CAGATGAAGA CAGCTGGGTA CCCCAACGTC
    AATATTCACA ATTTCACAAC CAGCTGGAGG GATGGCATGG CCTTCAATGC ACTGATACAC
    AAACACCGGC CTGACTTGAT AGATTTTGAC AAACTAAAGA AATCCAATGC ACACTACAAT
    CTGCAGAACG CCTTTAACCT GGCAGAGCAA CACCTTGGCC TCACTAAACT CTTGGACCCC
    GAAGATATCA GTGTGGACCA TCCTGATGAG AAGTCTATTA TCACTTACGT GGTGACTTAC
    TACCACTACT TCTCAAAGAT GAAGGCCTTG GCTGTTGAAG GAAAACGAAT TGGAAAGGTG
    CTTGACAACG CTATTGAAAC AGAAAAAATG ATTGAAAAGT ATGAGTCCCT TGCCTCTGAC
    CTTCTGGAGT GGATTGAACA AACTATCATT ATTTTGAACA ATCGCAAATT TGCCAACTCA
    CTCGTTGGGG TCCAGCAGCA GCTTCAGGCG TTCAACACTT ACCGCACTGT GGAGAAGCCC
    CCCAAATTTA CTGAGAAGGG GAACTTGGAA GTCCTGCTTT TCACCATTCA GAGCAAGATG
    AGGGCCAATA ACCAGAAGGT CTACATGCCC CGGGAGGGGA AGCTCATCTC TGACATTAAC
    AAGGCCTGGG AAAGACTGGA AAAAGCAGAA CACGAGCGAG AACTGGCCCT GCGAAATGAG
    CTCATAAGAC AGGAGAAACT GGAACAGCTC GCCCGCAGAT TTGACCGCAA GGCAGCTATG
    AGAGAGACCT GGCTGAGCGA GAACCAGCGT CTGGTGTCCC AGGACAACTT TGGATTTGAC
    CTTCCTGCTG TTGAGGCCGC CACGAAAAAG CATGAAGCCA TTGAGACTGA CATCGCTGCA
    TACGAGGAGC GCGTGCAGGC TGTGGTGGCT GTGGCCAGGG AACTGGAGGC AGAGAACTAC
    CACGACATCA AGCGCATCAC AGCACGGAAG GACAACGTCA TCCGGCTCTG GGAGTACCTG
    CTGGAGCTGC TCAGGGCCCG GAGACAGCGC CTCGAGATGA ACCTGGGGCT GCAGAAGATA
    TTCCAGGAAA TGCTGTACAT CATGGACTGG ATGGATGAAA TGAAGGTGCT GTTATTGTCT
    CAAGACTATG GCAAACACTT GCTCGGCGTG GAAGACCTGT TACAGAAGCA TGCCCTGGTT
    GAAGCAGACA TTGGCATCCA GGCAGAGCGG GTGAGAGGTG TCAACGCTTC TGCCCAGAAG
    TTTGCAACCG ACGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTAATCCG AGACAGGGTG
    GCCCACATGG AGTTCTGTTA CCAAGAGCTT TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC
    CTGGAAGAGT CCCGTCGCCT CTGGAAGTTC TTCTGGGAGA TGGCAGAGGA GGAGGGCTGG
    ATCCGGGAGA AGGAGAAGAT CCTGTCCTCT GATGACTACG GCAAGGACCT CACAAGCGTC
    ATGCGCCTGC TCAGCAAGCA CCGGGCATTC GAGGATGAGA TGAGCGGCCG CAGTGGCCAC
    TTCGAGCAAG CCATCAAGGA GGGCGAAGAC ATGATCGCCG AGGAGCACTT CGGGTCAGAG
    AAGATCCGGG AGCGGATTGC CTACATCCGC GAGCAGTGGG CCCACCTGGA GCAGCTCTCG
    GCCATCCGCA AGAAGCGCCT GGAGGAGGCC TCGCTGCTGC ACCAGTTCCA GGCGGATGCT
    GACGACATCG ATGCCTGGAT GCTGGACATC CTCAAGATCG TCTCCAGCAA CGACGTGGGC
    CACGATGAGT ACTCCACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC GGAGGAGATC
    GCCAACTACC GGCCCACCAT CGACTCGCTG CACGAGCAAG CCAGCGCCCT GCCCCAGGAG
    CACGCCGAGT CCCCCGACGT GCAGGGCCGG CTGGCGGGCA TTGAGGAGCG CTACAAGGAG
    GTGGCCGAGC TGACGCGGCT GCGGAAGCAG GCGCTGCAGG ACACTCTGGC CCTGTACAAG
    ATGTTCAGCG AGGCCGATGC CTGTGAGCTC TGGATTGACG AGAAGGAGCA GTGGCTCAAC
    AACATGCAGA TCCCAGAGAA GCTGGAAGAC CTGGAGGTCA TCCAGCACAG ATTCGAGAGC
    CTCGAACCGG AGATGAACAA CCAGGCTTCC CGGGTGGCAG TGGTGAACCA GATTGCCCGG
    CAGCTGATGC ACAGCGGCCA CCCAAGTGAG AAGGAGATCA AAGCCCAGCA GGACAAGCTC
    AACACGAGGT GGAGCCAGTT CAGGGAGCTG GTGGACCGGA AGAAGGATGC TCTCCTCTCC
    GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCATG GATCCGGGAG
    AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG TGTCATGGCC
    CTGCAGCGCA AGCTGACTGG CATGGAGCGG GACCTCGTGG CCATCGAGGC GAAACTGAGT
    GACCTGCAGA AGGAGGCCGA GAAGCTGGAG TCCGAGCACC CCGACCAGGC CCAGGCTATC
    CTGTCTCGGC TGGCCGAGAT CAGTGACGTG TGGGAGGAGA TGAAGACCAC GCTGAAGAAC
    CGTGAGGCCT CCCTGGGAGA GGCCAGCAAG CTGCAGCAGT TCCTGCGGGA CCTGGATGAC
    TTCCAGTCCT GGCTCTCCAG GACACAGACG GCCATTGCCT CGGAGGACAT GCCTAACACC
    CTGACCGAGG CTGAGAAGCT CCTCACGCAG CACGAGAACA TCAAAAACGA GATCGACAAC
    TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACCCA GGGCCAGACA
    GATGCCCAGT ACATGTTCCT GCGGCAGCGG CTGCAGGCCC TGGACACAGG CTGGAACGAG
    CTCCACAAGA TGTGGGAGAA CAGGCAGAGC CTCCTCTCCC AGTCCCACGC TCACCAGCAG
    TTCCTGCGGG ACACCAAGCA GGCCGAAGCC TTCCTGAACA ACCAGGAGTA TGTTTTGGCC
    CATACTGAAA TGCCCACCAC CTTGGAAGGA GCTGAAGCAG CTATTAAAAA ACAAGAGGAT
    TTCATGACCA CCATGGATGC CAATGAGGAG AAAATCAACG CGGTTGTGGA AACGGGCCGG
    AGGCTGGTGA GCGATGGGAA CATCAACTCG GATCGCATCC AGGAGAAGGT GGACTCCATT
    GATGACAGAC ACAGGAAGAA TCGCGAGGCA GCCAGTGAGC TTCTGATGAG ACTGAAGGAC
    AACAGGGATC TTCAGAAATT CCTGCAAGAC TGTCAGGAGC TGTCTCTCTG GATCAATGAA
    AAGATGCTTA CAGCCCAGGA CATGTCTTAC GATGAAGCCA GAAATCTGCA CAGTAAATGG
    TTGAAGCATC AAGCCTTTAT GGCAGAACTT GCTTCCAACA AAGAATGGCT TGACAAAATC
    GAGAAGGAGG GAATGCAGCT CATTTCAGAA AAGCCAGAGA CAGAAGCCGT GGTGAAGGAA
    AAACTCACAG GTTTACATAA AATGTGGGAA GTCCTTGAAT CCACCACACA GACCAAGGCC
    CAGCGGCTCT TCGATGCGAA CAAAGCCGAA CTCTTCACCC AGAGCTGTGC AGATCTGGAC
    AAATGGCTGC ATGGCCTGGA GAGTCAGATT CAGTCTGACG ACTATGGCAA AGACCTCACC
    AGCGTCAACA TCCTTCTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA GGTGCGGAAG
    AAGGAGATAG AAGAGCTCCA GAGCCAGGCG CAGGCCCTGA GCCAGGAGGG GAAGAGCACG
    GACGAGGTGG ACAGCAAGCG CCTCACCGTG CAGACCAAGT TCATGGAGCT GCTGGAGCCC
    CTCAACGAGA GGAAGCAGAA CCTGCTGGCC TCCAAGGAGA TCCATCAGTT CAACCGGGAC
    GTGGAGGACG AGATCTTGTG GGTTGGAGAG AGGATGCCTT TGGCGACCTC CACGGATCAC
    GGCCACAACC TCCAGACTGT GCAGTTGTTA ATAAAGAAAA ATCAGACCCT CCAGAAGGAG
    ATCCAGGGGC ACCAGCCTCG CATCGATGAC ATCTTTGAGA GGAGCCAGAA CATCATCACT
    GACAGCAGCC TCAACGCCGA GGCCATCCGG CAGAGACTGG CTGACCTGAA GCAGCTGTGG
    GGCCTCCTCA TCGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAGGC GCACAGGGCC
    CAGCAGTACT ACTTCGACGC GGCTGAGGCT GAGGCGTGGA TGAGCGAGCA GGAGCTGTAC
    ATGATGTCCG AGGAGAAGGC CAAGGATGAG CAGAGTGCCG TCTCCATGTT GAAGAAGCAC
    CAGATTCTGG AACAAGCTGT GGAGGACTAT GCGGAGACGG TGCACCAGCT CTCCAAGACC
    AGCCGGGCCC TGGTGGCCGA CAGCCATCCT GAAAGTGAAC GTATCAGTAT GCGGCAGTCC
    AAGGTGGACA AGCTGTATGC AGGCCTCAAG GACCTGGCCG AAGAGAGGCG GGGCAAGCTG
    GACGAGAGAC ACCGGCTGTT CCAGCTCAAC CGCGAGGTGG ACGACCTGGA GCAGTGGATT
    GCCGAGAGGG AGGTGGTCGC GGGGTCGCAT GAGCTGGGCC AGGACTACGA GCACGTCACG
    ATGCTCCAAG AGCGATTCCG AGAGTTCGCC CGGGACACGG GGAACATTGG GCAGGAACGC
    GTGGACACAG TCAACCACAT GGCAGACGAG CTCATCAACT CCGGACATTC AGATGCCGCT
    ACTATTGCTG AGTGGAAGGA TGGGCTCAAC GAGGCCTGGG CCGACCTCCT GGAGCTCATT
    GACACCAGAA CACAGATCCT CGCTGCCTCC TATGAACTGC ACAAGTTTTA CCACGACGCC
    AAGGAGATCT TTGGGCGCAT CCAGGACAAA CACAAGAAAC TCCCCGAGGA GCTCGGGAGA
    GATCAGAACA CAGTGGAGAC GTTACAGAGA ATGCACACCA CCTTTGAGCA CGACATCCAG
    GCCCTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGACGCGG CCCGCCTCCA GGCAGCCTAC
    GCGGGCGACA AGGCCGACGA CATCCAAAAA CGGGAAAACG AGGTGCTGGA AGCCTGGAAG
    GCCCTGCTAG ACGCCTGCGA AGGCCGCAGG GTACGGCTGG TGGACACGGG AGACAAGTTC
    CGCTTCTTCA GCATGGTGCG CGACCTCATG CTGTGGATGG AGGATGTCAT CCGGCAGATC
    GAAGCCCAGG AGAAGCCCCG GGATGTGTCA TCTGTTGAAC TATTGATGAA TAATCATCAA
    GGTATCAAAG CTGAAATCGA CGCTCGTAAT GACAGTTTTA CGACCTGCAT TGAACTGGGG
    AAATCCCTGC TGGCAAGGAA GCACTACGCA TCTGAGGAGA TCAAGGAGAA GTTACTGCAG
    TTGACAGAAA AGAGAAAAGA AATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGA
    CTGATCTTAG AGGTCCATCA GTTCTCAAGG GATGCCAGTG TGGCCGAGGC CTGGCTGCTC
    GGGCAGGAGC CCTACCTGTC CAGCCGAGAG ATAGGCCAGA GCGTGGACGA GGTTGAGAAG
    CTCATCAAGC GCCATGAGGC TTTTGAAAAG TCTGCAGCGA CCTGGGACGA GAGGTTCTCT
    GCCCTGGAAA GACTGACGAC GTTGGAGCTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG
    GAGAGGAAGA GGCGGCCGCC CTCCCCCGAG CCGAGCACGA AGGTTTCCGA GGAGACTGAG
    TCCCAGCAGC AGTGGGATAC TTCAAAAGGA GAGCAAGTTT CCCAGAATGG TTTGCCGGCT
    GAACAGGGAT CTCCACGGGT TAGTTACCGC TCTCAAACCT ACCAAAACTA CAAAAACTTT
    AATAGCAGAC GGACAGCCAG TGACCAGCCG TGGTCTGGAC TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.