iars-1 cDNA ORF clone, Caenorhabditis elegans

The following iars-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the iars-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCa344677 NM_069513.5
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Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
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OCa344677 NM_069513.4 Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCa344677
    Clone ID Related Accession (Same CDS sequence) NM_069513.5 , NM_069513.4
    Accession Version NM_069513.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-17
    Organism Caenorhabditis elegans
    Product Isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069513.4.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
    GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT
    CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT
    ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT
    GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG
    ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT
    GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT
    GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT
    GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT
    ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC
    AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG
    CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT
    CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT
    CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT
    GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC
    GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC
    GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT
    GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA
    TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG
    AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC
    TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC
    GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT
    GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA
    AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA
    TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT
    AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG
    GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT
    TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA
    GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC
    AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA
    AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA
    GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA
    TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC
    TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC
    ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC
    AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA
    CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA
    GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT
    CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG
    CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA
    AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT
    GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG
    AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA
    TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA
    TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA
    GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA
    TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT
    GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG
    ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA
    GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT
    GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC
    AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC
    GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT
    TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA
    GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT
    CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA
    TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA
    CGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_501914.1
    CDS9..3434
    Translation

    Target ORF information:

    RefSeq Version NM_069513.5
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_069513.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
    GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT
    CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT
    ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT
    GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG
    ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT
    GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT
    GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT
    GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT
    ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC
    AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG
    CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT
    CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT
    CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT
    GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC
    GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC
    GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT
    GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA
    TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG
    AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC
    TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC
    GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT
    GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA
    AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA
    TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT
    AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG
    GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT
    TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA
    GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC
    AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA
    AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA
    GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA
    TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC
    TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC
    ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC
    AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA
    CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA
    GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT
    CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG
    CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA
    AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT
    GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG
    AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA
    TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA
    TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA
    GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA
    TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT
    GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG
    ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA
    GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT
    GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC
    AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC
    GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT
    TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA
    GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT
    CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA
    TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA
    CGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCa344677
    Clone ID Related Accession (Same CDS sequence) NM_069513.5 , NM_069513.4
    Accession Version NM_069513.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3426bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-08
    Organism Caenorhabditis elegans
    Product Isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069513.3. COMPLETENESS: incomplete on both ends.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
    GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT
    CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT
    ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT
    GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG
    ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT
    GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT
    GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT
    GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT
    ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC
    AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG
    CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT
    CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT
    CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT
    GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC
    GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC
    GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT
    GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA
    TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG
    AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC
    TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC
    GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT
    GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA
    AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA
    TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT
    AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG
    GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT
    TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA
    GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC
    AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA
    AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA
    GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA
    TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC
    TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC
    ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC
    AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA
    CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA
    GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT
    CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG
    CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA
    AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT
    GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG
    AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA
    TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA
    TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA
    GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA
    TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT
    GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG
    ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA
    GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT
    GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC
    AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC
    GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT
    TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA
    GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT
    CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA
    TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA
    CGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_501914.1
    CDS1..3426
    Translation

    Target ORF information:

    RefSeq Version NM_069513.4
    Organism Caenorhabditis elegans
    Definition Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_069513.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
    GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT
    CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT
    ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT
    GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG
    ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT
    GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT
    GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT
    GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT
    ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC
    AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG
    CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT
    CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT
    CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT
    GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC
    GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC
    GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT
    GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA
    TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG
    AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC
    TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC
    GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT
    GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA
    AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA
    TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT
    AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG
    GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT
    TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA
    GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC
    AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA
    AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA
    GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA
    TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC
    TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC
    ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC
    AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA
    CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA
    GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT
    CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG
    CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA
    AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT
    GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG
    AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA
    TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA
    TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA
    GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA
    TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT
    GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG
    ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA
    GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT
    GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC
    AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC
    GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT
    TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA
    GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT
    CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA
    TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA
    CGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.