iars-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	iars-1
Entrez Gene ID	177926
Full Name	Isoleucine--tRNA ligase, cytoplasmic
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_069513.5	NP_501914.1	Isoleucine--tRNA ligase, cytoplasmic
NM_069513.4	NP_501914.1	Isoleucine--tRNA ligase, cytoplasmic

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following iars-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the iars-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa344677	NM_069513.5 Latest version!	Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa344677	NM_069513.4	Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

iars-1 ( NM_069513.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501914.1 Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), mRNA.

Price & Availability↑

CloneID	OCa344677
Clone ID Related Accession (Same CDS sequence)	NM_069513.5 , NM_069513.4
Accession Version	NM_069513.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_069513.4.

ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT 
CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT 
ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT 
GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG 
ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT 
GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT 
GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT 
GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT 
ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC 
AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG 
CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT 
CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT 
CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT 
GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC 
GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC 
GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT 
GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA 
TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG 
AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC 
TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC 
GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT 
GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA 
AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA 
TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT 
AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG 
GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT 
TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA 
GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC 
AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA 
AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA 
GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA 
TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC 
TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC 
ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC 
AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA 
CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA 
GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT 
CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG 
CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA 
AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT 
GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG 
AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA 
TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA 
TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA 
GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA 
TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT 
GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG 
ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA 
GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT 
GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC 
AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC 
GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT 
TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA 
GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT 
CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA 
TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA 
CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501914.1
CDS	9..3434
Translation	MSGLSTVPDNINFAREEDKVAQKWQDENTFQRSVELSKDRPHFTFYDGPPFATGLPHYGHMLTSTIKDVVGRWAHQNGHYVERRFGWDTHGLPVEYEIDKTLGISGPQDVMKMGIANYNNECRKIVMRYSGEWEKTMGRLGRWVDFKHDYKTLYPWFMESVWWAFSELHKKGLVYKGVKVMPFSTACSTPLSNFEAGQNYKDVVDPAVFVGFKLLDCPNRQLVAWTTTPWTLPSNLALVVHPDMLYVVTKDKTTGIEYVVLEERLGELKNDNLEVIEKLAGSQLKDLRYEPLFPYFAYMREERNAFRVLNDTFVTSDSGTGVVHQAPYFGEIDFQVCVANGVIAKDQKMICPVDESGKYTSEVPDYQGVYVKDADKLIIKRLKEMGNLVRQAEVKHSYPFCWRSDTPLLYKAVPSWFINVETLIPRLLANNDETYWVPAFVKEKRFANWLRDARDWAVSRNRFWGTPINLWVSEDGEEVVCVGSIAELEELSGQKITDLHRESVDDVTIPSRSGRGVLKRVSEVFDCWFESGSMPYAQNHYPFENRKIFEDNFPADFIAEGIDQTRGWFYTLLVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADALRLYLINSPVVRGENLRFREEGVRDLLKDVFLPWFNAYRFFVQNVQAYEHETGNVFDMNVHVASENVMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTKFFDTLTNIYIRLNRKRVKGDNGLHEQHHALAALGRVLILIVRLMAPFTPFFCEYIWLNLKKVIGSTEESVHFLMLPKPDESLIDETVERRVEVMRNVIDLVRLVRDREGLAVKYPLKEMIVINRDSQFLEDVKSLESYILLELNVRKLTVSQDKQKYGITLKAEPNFKILGARLKGEQKKVADYLKNQITESELEKFLLEGKLTVLGHELSSEEVAVSYAAGSDQGHGYKTHSDAKTIVMIDTTEDESLIEEGLCREVTNRVQRLRKQAKLVSTDTAHVHIVVSPADSQLAKVVAAKLNDIVSATGTPIKLGSPPAAAKAPTATSKSAIKDSEVELWLFAEGDSFDGITVVDGSKKVRVHVKTEKEHLKGYADLLYHVRSALDLWNGKIALKNVDGSAVHPTVDVTSLVGQTLQLVR

Target ORF information:

RefSeq Version	NM_069513.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069513.5

ORF Insert Sequence:

ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT 
CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT 
ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT 
GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG 
ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT 
GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT 
GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT 
GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT 
ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC 
AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG 
CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT 
CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT 
CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT 
GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC 
GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC 
GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT 
GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA 
TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG 
AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC 
TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC 
GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT 
GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA 
AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA 
TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT 
AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG 
GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT 
TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA 
GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC 
AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA 
AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA 
GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA 
TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC 
TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC 
ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC 
AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA 
CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA 
GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT 
CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG 
CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA 
AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT 
GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG 
AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA 
TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA 
TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA 
GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA 
TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT 
GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG 
ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA 
GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT 
GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC 
AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC 
GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT 
TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA 
GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT 
CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA 
TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA 
CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

iars-1 ( NM_069513.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501914.1 Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), partial mRNA.

Price & Availability↑

CloneID	OCa344677
Clone ID Related Accession (Same CDS sequence)	NM_069513.5 , NM_069513.4
Accession Version	NM_069513.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Jun 26, 2012 this sequence version replaced NM_069513.3. COMPLETENESS: incomplete on both ends.

ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT 
CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT 
ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT 
GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG 
ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT 
GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT 
GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT 
GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT 
ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC 
AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG 
CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT 
CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT 
CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT 
GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC 
GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC 
GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT 
GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA 
TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG 
AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC 
TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC 
GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT 
GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA 
AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA 
TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT 
AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG 
GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT 
TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA 
GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC 
AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA 
AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA 
GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA 
TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC 
TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC 
ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC 
AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA 
CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA 
GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT 
CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG 
CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA 
AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT 
GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG 
AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA 
TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA 
TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA 
GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA 
TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT 
GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG 
ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA 
GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT 
GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC 
AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC 
GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT 
TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA 
GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT 
CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA 
TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA 
CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501914.1
CDS	1..3426
Translation	MSGLSTVPDNINFAREEDKVAQKWQDENTFQRSVELSKDRPHFTFYDGPPFATGLPHYGHMLTSTIKDVVGRWAHQNGHYVERRFGWDTHGLPVEYEIDKTLGISGPQDVMKMGIANYNNECRKIVMRYSGEWEKTMGRLGRWVDFKHDYKTLYPWFMESVWWAFSELHKKGLVYKGVKVMPFSTACSTPLSNFEAGQNYKDVVDPAVFVGFKLLDCPNRQLVAWTTTPWTLPSNLALVVHPDMLYVVTKDKTTGIEYVVLEERLGELKNDNLEVIEKLAGSQLKDLRYEPLFPYFAYMREERNAFRVLNDTFVTSDSGTGVVHQAPYFGEIDFQVCVANGVIAKDQKMICPVDESGKYTSEVPDYQGVYVKDADKLIIKRLKEMGNLVRQAEVKHSYPFCWRSDTPLLYKAVPSWFINVETLIPRLLANNDETYWVPAFVKEKRFANWLRDARDWAVSRNRFWGTPINLWVSEDGEEVVCVGSIAELEELSGQKITDLHRESVDDVTIPSRSGRGVLKRVSEVFDCWFESGSMPYAQNHYPFENRKIFEDNFPADFIAEGIDQTRGWFYTLLVLSTALFNKPPFKNLICNGLVLASDGAKMSKSKKNYPDPMLIVNKYGADALRLYLINSPVVRGENLRFREEGVRDLLKDVFLPWFNAYRFFVQNVQAYEHETGNVFDMNVHVASENVMDRWIESFTNSLVAFVRKEMDSYRLYAVVGPLTKFFDTLTNIYIRLNRKRVKGDNGLHEQHHALAALGRVLILIVRLMAPFTPFFCEYIWLNLKKVIGSTEESVHFLMLPKPDESLIDETVERRVEVMRNVIDLVRLVRDREGLAVKYPLKEMIVINRDSQFLEDVKSLESYILLELNVRKLTVSQDKQKYGITLKAEPNFKILGARLKGEQKKVADYLKNQITESELEKFLLEGKLTVLGHELSSEEVAVSYAAGSDQGHGYKTHSDAKTIVMIDTTEDESLIEEGLCREVTNRVQRLRKQAKLVSTDTAHVHIVVSPADSQLAKVVAAKLNDIVSATGTPIKLGSPPAAAKAPTATSKSAIKDSEVELWLFAEGDSFDGITVVDGSKKVRVHVKTEKEHLKGYADLLYHVRSALDLWNGKIALKNVDGSAVHPTVDVTSLVGQTLQLVR

Target ORF information:

RefSeq Version	NM_069513.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Isoleucine--tRNA ligase, cytoplasmic (iars-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_069513.4

ORF Insert Sequence:

ATGAGCGGCC TTTCGACCGT TCCCGACAAC ATCAATTTTG CCAGAGAAGA AGATAAAGTC 
GCTCAAAAAT GGCAAGATGA GAATACTTTC CAACGTTCTG TTGAGCTGTC AAAAGATCGT 
CCACATTTCA CTTTTTATGA TGGACCACCT TTCGCTACTG GCCTTCCTCA TTATGGTCAT 
ATGCTTACCA GCACCATCAA AGACGTTGTT GGTCGCTGGG CTCATCAAAA CGGTCACTAT 
GTAGAAAGAC GGTTTGGTTG GGATACTCAT GGTCTTCCAG TCGAATACGA AATCGATAAG 
ACGCTTGGAA TCAGTGGTCC ACAAGATGTC ATGAAAATGG GAATCGCTAA TTACAACAAT 
GAATGCCGAA AGATCGTTAT GAGATATTCT GGCGAATGGG AGAAAACAAT GGGACGTCTT 
GGTCGCTGGG TAGACTTCAA GCATGATTAC AAGACTCTCT ACCCGTGGTT CATGGAGTCT 
GTATGGTGGG CTTTCAGCGA GTTGCATAAG AAGGGCCTTG TCTACAAAGG TGTAAAAGTT 
ATGCCTTTCT CTACTGCTTG TTCTACTCCA CTCTCCAACT TTGAAGCTGG TCAGAATTAC 
AAAGATGTAG TAGATCCAGC AGTATTCGTC GGATTCAAAT TACTCGATTG TCCGAATAGG 
CAGCTAGTTG CATGGACAAC AACTCCATGG ACACTTCCAT CCAATCTAGC TCTAGTTGTT 
CATCCAGACA TGCTGTATGT GGTCACTAAA GATAAAACGA CTGGAATCGA ATACGTTGTT 
CTGGAAGAAC GTCTCGGAGA GTTGAAAAAT GATAATTTGG AAGTTATTGA GAAACTTGCT 
GGTAGTCAGC TCAAAGATCT CAGGTATGAA CCATTGTTCC CATACTTTGC CTATATGCGC 
GAAGAAAGAA ACGCATTCCG GGTATTGAAT GACACTTTTG TCACCTCTGA CTCTGGTACC 
GGAGTTGTTC ATCAAGCACC ATATTTCGGA GAGATCGATT TCCAAGTTTG TGTGGCGAAT 
GGAGTTATCG CTAAAGACCA AAAAATGATC TGTCCAGTTG ATGAAAGTGG AAAATACACA 
TCAGAAGTTC CAGATTATCA AGGAGTCTAC GTGAAAGATG CTGATAAGTT GATTATCAAG 
AGACTCAAGG AAATGGGAAA TCTTGTCAGA CAAGCGGAAG TGAAGCACTC CTATCCATTC 
TGTTGGCGGT CTGACACTCC ACTTCTCTAT AAGGCTGTTC CATCCTGGTT CATCAACGTC 
GAAACCCTGA TTCCAAGGCT TCTGGCTAAC AATGATGAGA CATACTGGGT TCCCGCTTTT 
GTCAAGGAAA AGAGATTTGC TAACTGGCTT AGAGATGCTC GCGATTGGGC TGTATCGAGA 
AATCGGTTCT GGGGAACTCC AATCAATTTG TGGGTGAGCG AAGATGGAGA GGAAGTTGTA 
TGTGTTGGAA GTATTGCTGA ATTAGAAGAG CTTTCAGGAC AAAAAATCAC TGATCTTCAT 
AGAGAAAGCG TTGATGATGT TACGATCCCA TCTCGTTCAG GACGGGGAGT TCTCAAGAGG 
GTTTCCGAAG TTTTTGATTG TTGGTTTGAA TCAGGATCCA TGCCATATGC CCAGAATCAT 
TATCCATTTG AGAACCGCAA AATCTTCGAA GACAATTTCC CAGCTGATTT CATCGCTGAA 
GGAATTGACC AAACCCGTGG GTGGTTTTAC ACACTTCTAG TTCTGTCAAC TGCTTTGTTC 
AACAAGCCAC CATTCAAGAA CCTGATCTGT AACGGACTCG TATTGGCGTC GGATGGAGCA 
AAAATGTCAA AGAGTAAGAA GAACTATCCC GATCCAATGC TCATTGTTAA CAAGTACGGA 
GCCGATGCTT TGCGTCTTTA TCTCATCAAC TCTCCTGTGG TTAGAGGAGA AAATTTGAGA 
TTCAGAGAAG AAGGCGTAAG AGATCTTCTC AAGGATGTGT TCCTTCCATG GTTTAATGCC 
TACAGATTCT TTGTACAAAA CGTACAAGCA TATGAACATG AAACTGGAAA TGTGTTTGAC 
ATGAACGTTC ATGTTGCCAG TGAAAATGTC ATGGATCGGT GGATTGAATC CTTTACAAAC 
AGTCTTGTTG CGTTTGTTCG GAAAGAAATG GATAGTTATC GGCTTTATGC TGTTGTTGGA 
CCGCTTACAA AATTCTTTGA CACTCTTACA AATATCTACA TCAGATTAAA TAGAAAGAGA 
GTCAAGGGAG ACAACGGACT TCATGAGCAA CATCACGCTT TGGCAGCCCT CGGACGAGTT 
CTCATTTTGA TTGTTAGATT GATGGCTCCT TTCACACCAT TCTTCTGCGA GTATATCTGG 
CTAAATTTGA AGAAAGTCAT TGGTTCGACT GAGGAATCAG TTCACTTCCT GATGCTCCCA 
AAACCGGATG AGAGTCTAAT CGACGAGACT GTGGAAAGAC GAGTTGAAGT TATGAGAAAT 
GTTATCGACT TGGTTCGTCT TGTTCGCGAT CGTGAAGGAC TTGCTGTGAA ATATCCATTG 
AAGGAAATGA TTGTTATTAA CAGAGACAGT CAGTTTTTGG AAGACGTAAA AAGTTTGGAA 
TCATATATTC TTCTTGAGTT GAACGTCAGA AAATTGACCG TTTCACAAGA TAAACAGAAA 
TACGGAATCA CCCTAAAGGC AGAGCCAAAC TTCAAAATTC TGGGTGCTCG TCTCAAGGGA 
GAACAAAAGA AGGTTGCTGA CTACTTGAAG AACCAAATCA CTGAATCTGA GTTGGAGAAA 
TTCCTTCTTG AAGGAAAATT GACTGTGCTT GGGCATGAGC TCTCCTCGGA AGAAGTTGCT 
GTTAGCTACG CGGCTGGAAG TGATCAAGGA CATGGATACA AAACTCACTC GGATGCAAAG 
ACCATTGTAA TGATTGATAC AACTGAAGAT GAGTCTCTTA TTGAAGAAGG ACTCTGCAGA 
GAAGTGACAA ATCGTGTTCA ACGTCTTCGC AAACAGGCTA AACTTGTATC TACTGATACT 
GCTCATGTTC ATATTGTTGT TAGTCCAGCT GATTCTCAAC TCGCAAAGGT TGTTGCTGCC 
AAATTGAATG ACATTGTATC CGCTACTGGA ACACCTATCA AGTTGGGATC ACCACCAGCC 
GCTGCAAAGG CTCCAACTGC CACCAGCAAG AGTGCAATTA AGGATTCAGA AGTTGAGCTT 
TGGTTGTTCG CCGAAGGTGA CAGTTTCGAT GGGATCACTG TTGTTGATGG ATCAAAGAAA 
GTTCGAGTTC ATGTGAAAAC AGAAAAAGAA CACCTGAAGG GATATGCTGA TCTTCTATAT 
CACGTTCGCT CTGCTCTTGA TCTCTGGAAC GGTAAGATTG CCTTGAAGAA TGTCGATGGA 
TCGGCGGTCC ATCCAACAGT TGACGTGACG TCTCTTGTTG GTCAGACTCT TCAACTCGTA 
CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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iars-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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