IARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS1
Entrez Gene ID	3376
Full Name	isoleucyl-tRNA synthetase
Synonyms	GRIDHH,IARS1,ILERS,ILRS,IRS,PRO0785
General protein information	Preferred Names isoleucyl-tRNA synthetase Names isoleucine--tRNA ligase, cytoplasmic isoleucine tRNA ligase 1, cytoplasmic
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q22.31
Summary	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. Alternatively spliced transcript variants have been found. [provided by RefSeq, Nov 2012].
Disorder MIM:	600709
Disorder Html:	Growth retardation, intellectual developmental disorder, hypotonia, and hepatopathy, 617093 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001374300.1	NP_001361229.1	isoleucine--tRNA ligase, cytoplasmic isoform 2
NM_013417.3	NP_038203.2	isoleucine--tRNA ligase, cytoplasmic
NM_013417.4	NP_038203.2	isoleucine--tRNA ligase, cytoplasmic isoform 1
NM_001374301.1	NP_001361230.1	isoleucine--tRNA ligase, cytoplasmic isoform 3
NM_002161.5	NP_002152.2	isoleucine--tRNA ligase, cytoplasmic
NM_002161.6	NP_002152.2	isoleucine--tRNA ligase, cytoplasmic isoform 1
NM_001374299.1	NP_001361228.1	isoleucine--tRNA ligase, cytoplasmic isoform 2

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology
Bibliography

Related articles in PubMed

New evidence for association of recessive IARS gene mutations with hepatopathy, hypotonia, intellectual disability and growth retardation.
Smigiel R, Biela M, Biernacka A, Stembalska A, Sasiadek M, Kosinska J, Rydzanicz M, Ploski R
Clinical genetics92(6)671-673(2017 Dec)

Bi-allelic IARS mutations in a child with intra-uterine growth retardation, neonatal cholestasis, and mild developmental delay.
Orenstein N, Weiss K, Oprescu SN, Shapira R, Kidron D, Vanagaite-Basel L, Antonellis A, Muenke M
Clinical genetics91(6)913-917(2017 Jun)

Biallelic IARS Mutations Cause Growth Retardation with Prenatal Onset, Intellectual Disability, Muscular Hypotonia, and Infantile Hepatopathy.
Kopajtich R, Murayama K, Janecke AR, Haack TB, Breuer M, Knisely AS, Harting I, Ohashi T, Okazaki Y, Watanabe D, Tokuzawa Y, Kotzaeridou U, K?lker S, Sauer S, Carl M, Straub S, Entenmann A, Gizewski E, Feichtinger RG, Mayr JA, Lackner K, Strom TM, Meitinger T, M?ller T, Ohtake A, Hoffmann GF, Prokisch H, Staufner C
American journal of human genetics99(2)414-22(2016 Aug)

Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton.
Forster F, Paster W, Supper V, Schatzlmaier P, Sunzenauer S, Ostler N, Saliba A, Eckerstorfer P, Britzen-Laurent N, Sch?tz G, Schmid JA, Zlabinger GJ, Naschberger E, St?rzl M, Stockinger H
Journal of immunology (Baltimore, Md. : 1950)192(2)771-81(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

bi-allelic mutations in cytosolic isoleucyl-tRNA synthetase (IARS) have been described in three individuals with growth delay, hepatic dysfunction, and neurodevelopmental disabilities. Here we report an additional subject with this condition identified by whole-exome sequencing. Our findings support the association between this disorder and neonatal cholestasis with distinct liver pathology
Title: Bi-allelic IARS mutations in a child with intra-uterine growth retardation, neonatal cholestasis, and mild developmental delay.

Biallelic IARS Mutations Cause Growth Retardation with Prenatal Onset, Intellectual Disability, Muscular Hypotonia, and Infantile Hepatopathy
Title: Biallelic IARS Mutations Cause Growth Retardation with Prenatal Onset, Intellectual Disability, Muscular Hypotonia, and Infantile Hepatopathy.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following IARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu108895	NM_001374299.1 Latest version!	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu108895	NM_001374300.1 Latest version!	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu108896	NM_001374301.1 Latest version!	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu24821	NM_013417.4 Latest version!	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu24821	NM_002161.6 Latest version!	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OHu24821	NM_013417.3	Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu24821	NM_002161.5	Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS1 ( NM_001374300.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361229.1 Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu108895
Clone ID Related Accession (Same CDS sequence)	NM_001374300.1 , NM_001374299.1
Accession Version	NM_001374300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.146576.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG 
CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG 
CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT 
CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT 
TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA 
AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG 
AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG 
CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC 
CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA 
GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA 
ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA 
ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT 
ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA 
GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG 
GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT 
AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT 
GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA 
AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG 
AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT 
TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG 
TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC 
ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA 
AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT 
TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361229.1
CDS	265..3978
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_001374300.1
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374300.1

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG 
CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG 
CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT 
CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT 
TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA 
AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG 
AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG 
CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC 
CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA 
GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA 
ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA 
ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT 
ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA 
GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG 
GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT 
AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT 
GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA 
AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG 
AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT 
TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG 
TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC 
ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA 
AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT 
TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS1 ( NM_001374299.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361228.1 Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu108895
Clone ID Related Accession (Same CDS sequence)	NM_001374300.1 , NM_001374299.1
Accession Version	NM_001374299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.153835.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG 
CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG 
CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT 
CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT 
TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA 
AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG 
AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG 
CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC 
CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA 
GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA 
ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA 
ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT 
ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA 
GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG 
GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT 
AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT 
GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA 
AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG 
AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT 
TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG 
TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC 
ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA 
AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT 
TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361228.1
CDS	95..3808
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_001374299.1
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374299.1

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGAC AAGGACACAC TCAGCATTCA CTACCTCATG 
CTGCCCCGTG TTCGAGAAGA ATTGATTGAC AAGAAAACAG AGAGTGCAGT ATCTCAGATG 
CAGTCTGTGA TTGAACTTGG AAGAGTGATC AGAGACCGAA AAACTATTCC CATAAAGTAT 
CCTTTGAAAG AAATTGTGGT TATCCATCAA GATCCAGAAG CTCTTAAAGA TATCAAGTCT 
TTGGAGAAGT ATATCATTGA GGAACTCAAT GTTCGAAAAG TTACACTGTC TACAGATAAA 
AACAAGTATG GCATTCGGCT AAGGGCAGAA CCAGATCACA TGGTCCTGGG GAAGCGTCTG 
AAGGGAGCCT TTAAGGCAGT GATGACGTCC ATCAAGCAGT TGAGCAGTGA GGAGCTGGAG 
CAGTTCCAGA AGACTGGGAC CATTGTTGTG GAAGGCCATG AATTGCACGA TGAAGACATC 
CGCCTCATGT ACACCTTTGA TCAGGCCACA GGTGGGACTG CGCAATTTGA AGCACACTCA 
GATGCTCAGG CTTTGGTCCT CTTAGATGTC ACTCCTGACC AGTCAATGGT AGATGAAGGA 
ATGGCTCGGG AAGTCATCAA TCGCATACAG AAACTTCGCA AAAAGTGCAA TCTGGTTCCA 
ACTGATGAAA TCACAGTGTA CTATAAAGCA AAGTCTGAAG GAACATATCT GAATAGTGTT 
ATTGAAAGCC ACACAGAGTT CATATTTACC ACCATAAAGG CTCCCTTGAA ACCATATCCA 
GTTTCTCCAT CGGATAAAGT CCTTATTCAA GAAAAAACAC AGTTGAAGGG ATCTGAACTG 
GAAATTACAC TCACCAGAGG ATCTTCCCTT CCTGGTCCTG CTTGTGCATA TGTCAATCTT 
AACATTTGTG CAAATGGCAG TGAACAAGGT GGAGTATTGC TCCTGGAAAA TCCAAAAGGT 
GACAATAGGT TGGACCTTTT AAAGCTGAAG AGTGTTGTCA CTAGCATTTT TGGTGTGAAA 
AATACAGAGC TGGCTGTCTT CCATGATGAA ACAGAAATAC AAAACCAAAC TGACTTACTG 
AGTCTTAGTG GAAAAACACT TTGTGTGACT GCAGGATCGG CTCCCTCTCT GATCAACAGT 
TCTAGTACTC TTCTTTGTCA GTATATCAAC CTACAGCTCC TGAATGCAAA GCCACAAGAG 
TGTTTAATGG GGACAGTGGG CACTCTCCTG CTTGAAAACC CACTTGGGCA GAATGGACTC 
ACCCACCAAG GTCTTCTGTA TGAAGCAGCC AAGGTGTTTG GCCTTCGGAG CAGGAAGCTA 
AAGCTGTTTC TGAATGAGAC CCAAACGCAG GAAATTACAG AAGACATCCC CGTGAAGACT 
TTGAATATGA AGACTGTGTA TGTTTCTGTG TTACCAACAA CAGCAGACTT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS1 ( NM_001374301.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361230.1 Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 6, mRNA.

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CloneID	OHu108896
Clone ID Related Accession (Same CDS sequence)	NM_001374301.1
Accession Version	NM_001374301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.129230.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGATT ATATCTGATT 
AACTCCCCTG TGGTGAGAGC AGAAAACCTC CGCTTTAAAG AAGAGGGTGT GCGGGACGTC 
CTTAAGGATG TACTGCTCCC ATGGTACAAT GCCTATCGCT TCTTAATCCA GAACGTTCTG 
AGGCTCCAGA AGGAGGAAGA AATAGAATTT CTCTACAATG AGAACACGGT TAGAGAAAGC 
CCCAACATTA CAGACCGGTG GATCCTGTCC TTCATGCAGT CTCTCATTGG CTTCTTTGAG 
ACTGAAATGG CAGCTTATAG GCTTTATACT GTGGTGCCTC GCCTGGTCAA GTTTGTAGAT 
ATTCTGACCA ATTGGTATGT TAGAATGAAC CGCAGAAGAT TAAAGGGTGA AAATGGGATG 
GAGGATTGTG TCATGGCCCT AGAAACCTTG TTTAGTGTTC TGCTTTCTCT TTGCAGACTT 
ATGGCTCCCT ACACACCTTT TCTCACTGAA TTGATGTACC AGAATCTAAA GGTGCTGATT 
GACCCTGTTT CTGTTCAGGA CAAGGACACA CTCAGCATTC ACTACCTCAT GCTGCCCCGT 
GTTCGAGAAG AATTGATTGA CAAGAAAACA GAGAGTGCAG TATCTCAGAT GCAGTCTGTG 
ATTGAACTTG GAAGAGTGAT CAGAGACCGA AAAACTATTC CCATAAAGTA TCCTTTGAAA 
GAAATTGTGG TTATCCATCA AGATCCAGAA GCTCTTAAAG ATATCAAGTC TTTGGAGAAG 
TATATCATTG AGGAACTCAA TGTTCGAAAA GTTACACTGT CTACAGATAA AAACAAGTAT 
GGCATTCGGC TAAGGGCAGA ACCAGATCAC ATGGTCCTGG GGAAGCGTCT GAAGGGAGCC 
TTTAAGGCAG TGATGACGTC CATCAAGCAG TTGAGCAGTG AGGAGCTGGA GCAGTTCCAG 
AAGACTGGGA CCATTGTTGT GGAAGGCCAT GAATTGCACG ATGAAGACAT CCGCCTCATG 
TACACCTTTG ATCAGGCCAC AGGTGGGACT GCGCAATTTG AAGCACACTC AGATGCTCAG 
GCTTTGGTCC TCTTAGATGT CACTCCTGAC CAGTCAATGG TAGATGAAGG AATGGCTCGG 
GAAGTCATCA ATCGCATACA GAAACTTCGC AAAAAGTGCA ATCTGGTTCC AACTGATGAA 
ATCACAGTGT ACTATAAAGC AAAGTCTGAA GGAACATATC TGAATAGTGT TATTGAAAGC 
CACACAGAGT TCATATTTAC CACCATAAAG GCTCCCTTGA AACCATATCC AGTTTCTCCA 
TCGGATAAAG TCCTTATTCA AGAAAAAACA CAGTTGAAGG GATCTGAACT GGAAATTACA 
CTCACCAGAG GATCTTCCCT TCCTGGTCCT GCTTGTGCAT ATGTCAATCT TAACATTTGT 
GCAAATGGCA GTGAACAAGG TGGAGTATTG CTCCTGGAAA ATCCAAAAGG TGACAATAGG 
TTGGACCTTT TAAAGCTGAA GAGTGTTGTC ACTAGCATTT TTGGTGTGAA AAATACAGAG 
CTGGCTGTCT TCCATGATGA AACAGAAATA CAAAACCAAA CTGACTTACT GAGTCTTAGT 
GGAAAAACAC TTTGTGTGAC TGCAGGATCG GCTCCCTCTC TGATCAACAG TTCTAGTACT 
CTTCTTTGTC AGTATATCAA CCTACAGCTC CTGAATGCAA AGCCACAAGA GTGTTTAATG 
GGGACAGTGG GCACTCTCCT GCTTGAAAAC CCACTTGGGC AGAATGGACT CACCCACCAA 
GGTCTTCTGT ATGAAGCAGC CAAGGTGTTT GGCCTTCGGA GCAGGAAGCT AAAGCTGTTT 
CTGAATGAGA CCCAAACGCA GGAAATTACA GAAGACATCC CCGTGAAGAC TTTGAATATG 
AAGACTGTGT ATGTTTCTGT GTTACCAACA ACAGCAGACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361230.1
CDS	95..3799
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLARLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_001374301.1
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374301.1

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGATT ATATCTGATT 
AACTCCCCTG TGGTGAGAGC AGAAAACCTC CGCTTTAAAG AAGAGGGTGT GCGGGACGTC 
CTTAAGGATG TACTGCTCCC ATGGTACAAT GCCTATCGCT TCTTAATCCA GAACGTTCTG 
AGGCTCCAGA AGGAGGAAGA AATAGAATTT CTCTACAATG AGAACACGGT TAGAGAAAGC 
CCCAACATTA CAGACCGGTG GATCCTGTCC TTCATGCAGT CTCTCATTGG CTTCTTTGAG 
ACTGAAATGG CAGCTTATAG GCTTTATACT GTGGTGCCTC GCCTGGTCAA GTTTGTAGAT 
ATTCTGACCA ATTGGTATGT TAGAATGAAC CGCAGAAGAT TAAAGGGTGA AAATGGGATG 
GAGGATTGTG TCATGGCCCT AGAAACCTTG TTTAGTGTTC TGCTTTCTCT TTGCAGACTT 
ATGGCTCCCT ACACACCTTT TCTCACTGAA TTGATGTACC AGAATCTAAA GGTGCTGATT 
GACCCTGTTT CTGTTCAGGA CAAGGACACA CTCAGCATTC ACTACCTCAT GCTGCCCCGT 
GTTCGAGAAG AATTGATTGA CAAGAAAACA GAGAGTGCAG TATCTCAGAT GCAGTCTGTG 
ATTGAACTTG GAAGAGTGAT CAGAGACCGA AAAACTATTC CCATAAAGTA TCCTTTGAAA 
GAAATTGTGG TTATCCATCA AGATCCAGAA GCTCTTAAAG ATATCAAGTC TTTGGAGAAG 
TATATCATTG AGGAACTCAA TGTTCGAAAA GTTACACTGT CTACAGATAA AAACAAGTAT 
GGCATTCGGC TAAGGGCAGA ACCAGATCAC ATGGTCCTGG GGAAGCGTCT GAAGGGAGCC 
TTTAAGGCAG TGATGACGTC CATCAAGCAG TTGAGCAGTG AGGAGCTGGA GCAGTTCCAG 
AAGACTGGGA CCATTGTTGT GGAAGGCCAT GAATTGCACG ATGAAGACAT CCGCCTCATG 
TACACCTTTG ATCAGGCCAC AGGTGGGACT GCGCAATTTG AAGCACACTC AGATGCTCAG 
GCTTTGGTCC TCTTAGATGT CACTCCTGAC CAGTCAATGG TAGATGAAGG AATGGCTCGG 
GAAGTCATCA ATCGCATACA GAAACTTCGC AAAAAGTGCA ATCTGGTTCC AACTGATGAA 
ATCACAGTGT ACTATAAAGC AAAGTCTGAA GGAACATATC TGAATAGTGT TATTGAAAGC 
CACACAGAGT TCATATTTAC CACCATAAAG GCTCCCTTGA AACCATATCC AGTTTCTCCA 
TCGGATAAAG TCCTTATTCA AGAAAAAACA CAGTTGAAGG GATCTGAACT GGAAATTACA 
CTCACCAGAG GATCTTCCCT TCCTGGTCCT GCTTGTGCAT ATGTCAATCT TAACATTTGT 
GCAAATGGCA GTGAACAAGG TGGAGTATTG CTCCTGGAAA ATCCAAAAGG TGACAATAGG 
TTGGACCTTT TAAAGCTGAA GAGTGTTGTC ACTAGCATTT TTGGTGTGAA AAATACAGAG 
CTGGCTGTCT TCCATGATGA AACAGAAATA CAAAACCAAA CTGACTTACT GAGTCTTAGT 
GGAAAAACAC TTTGTGTGAC TGCAGGATCG GCTCCCTCTC TGATCAACAG TTCTAGTACT 
CTTCTTTGTC AGTATATCAA CCTACAGCTC CTGAATGCAA AGCCACAAGA GTGTTTAATG 
GGGACAGTGG GCACTCTCCT GCTTGAAAAC CCACTTGGGC AGAATGGACT CACCCACCAA 
GGTCTTCTGT ATGAAGCAGC CAAGGTGTTT GGCCTTCGGA GCAGGAAGCT AAAGCTGTTT 
CTGAATGAGA CCCAAACGCA GGAAATTACA GAAGACATCC CCGTGAAGAC TTTGAATATG 
AAGACTGTGT ATGTTTCTGT GTTACCAACA ACAGCAGACT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS1 ( NM_013417.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_038203.2 Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24821
Clone ID Related Accession (Same CDS sequence)	NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
Accession Version	NM_013417.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. On Sep 19, 2019 this sequence version replaced NM_013417.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D28473.1, U04953.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038203.2
CDS	265..4053
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_013417.4
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013417.4

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS1 ( NM_002161.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002152.2 Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu24821
Clone ID Related Accession (Same CDS sequence)	NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
Accession Version	NM_002161.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL136097.10. On Oct 11, 2019 this sequence version replaced NM_002161.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293014.1, BX647236.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002152.2
CDS	95..3883
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_002161.6
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase 1 (IARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002161.6

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS1 ( NM_013417.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_038203.2 Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 2, mRNA.

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CloneID	OHu24821
Clone ID Related Accession (Same CDS sequence)	NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
Accession Version	NM_013417.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA335066.1, D28473.1 and AA731323.1. This sequence is a reference standard in the RefSeqGene project. On Nov 21, 2012 this sequence version replaced NM_013417.2. Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.169974.1, SRR1803616.208815.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038203.2
CDS	324..4112
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_013417.3
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013417.3

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS1 ( NM_002161.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002152.2 Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 1, mRNA.

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CloneID	OHu24821
Clone ID Related Accession (Same CDS sequence)	NM_013417.3 , NM_013417.4 , NM_002161.5 , NM_002161.6
Accession Version	NM_002161.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA335066.1, BC065552.1, BC008318.1 and AA731323.1. On Nov 21, 2012 this sequence version replaced NM_002161.4. Transcript Variant: This variant (1) and variant 2 encode the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.29740.1, SRR1803616.98679.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002152.2
CDS	154..3942
Translation	MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_002161.5
Organism	Homo sapiens(human)
Definition	Homo sapiens isoleucyl-tRNA synthetase (IARS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002161.5

ORF Insert Sequence:

ATGCTTCAAC AAGTTCCAGA AAACATAAAT TTTCCTGCTG AAGAAGAGAA AATCTTGGAG 
TTTTGGACTG AATTTAATTG TTTTCAGGAA TGCTTAAAGC AATCAAAACA TAAACCAAAA 
TTTACCTTCT ATGATGGTCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCGGGTACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCTTA CCTGTGGAAT ATGAAATTGA TAAGACACTG 
GGAATCAGAG GACCAGAGGA TGTGGCCAAA ATGGGGATTA CAGAGTATAA CAATCAGTGC 
CGAGCAATTG TGATGAGATA TTCTGCTGAG TGGAAGTCTA CTGTTAGCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTATAAAACT CTGTATCCAC AATTCATGGA ATCAGTCTGG 
TGGGTCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCTACGG CATGTAACAC TCCACTTTCC AACTTCGAGT CACACCAGAA TTATAAGGAT 
GTTCAAGATC CTTCAGTATT TGTAACTTTC CCTTTGGAAG AAGATGAAAC TGTATCTTTA 
GTTGCTTGGA CAACCACTCC CTGGACTCTA CCTAGTAACC TTGCTGTGTG TGTTAATCCA 
GAAATGCAAT ATGTGAAAAT TAAAGATGTT GCCAGAGGAC GATTACTCAT TTTAATGGAA 
GCCAGATTGT CAGCCCTCTA TAAATTGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTATC TTAAAGGCAA GAAGTACAGG CCCCTGTTTG ACTATTTCCT GAAGTGTAAA 
GAGAATGGCG CTTTCACTGT GCTTGTTGAC AACTATGTGA AGGAAGAAGA AGGCACAGGG 
GTTGTCCACC AAGCTCCTTA CTTCGGTGCT GAGGACTATC GGGTCTGTAT GGACTTTAAC 
ATTATTCGGA AAGACTCACT CCCTGTTTGC CCTGTGGATG CTTCAGGCTG CTTCACAACG 
GAGGTGACAG ATTTCGCAGG ACAGTATGTG AAGGATGCTG ACAAAAGTAT CATCAGGACT 
TTGAAGGAAC AAGGCCGACT TCTGGTTGCC ACCACCTTCA CTCACAGCTA CCCTTTTTGC 
TGGAGATCAG ACACTCCTCT AATTTACAAA GCAGTGCCCA GCTGGTTTGT GCGAGTGGAG 
AACATGGTGG ACCAGCTCCT AAGGAACAAT GACCTGTGCT ACTGGGTCCC AGAGTTGGTA 
CGAGAAAAAC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGACAAT TTCCAGAAAC 
AGATACTGGG GCACCCCCAT CCCACTGTGG GTCAGCGATG ACTTTGAGGA GGTGGTATGC 
ATTGGGTCAG TGGCGGAACT TGAAGAACTG TCAGGAGCAA AGATCTCAGA TCTCCACAGA 
GAGAGTGTTG ACCACCTGAC CATTCCTTCA CGCTGTGGGA AGGGATCCTT GCACCGCATC 
TCTGAAGTGT TTGACTGTTG GTTTGAGAGT GGCAGCATGC CCTATGCTCA GGTTCATTAC 
CCGTTTGAAA ACAAGAGGGA GTTTGAGGAT GCTTTTCCTG CAGATTTCAT TGCCGAGGGC 
ATCGACCAAA CCAGAGGATG GTTTTATACC CTGCTGGTGC TGGCCACGGC CCTCTTTGGA 
CAACCGCCTT TCAAGAACGT AATTGTGAAT GGGCTTGTCC TGGCAAGTGA TGGCCAAAAA 
ATGAGCAAAC GGAAAAAGAA TTATCCAGAT CCAGTTTCCA TCATCCAGAA GTATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTCCGCTTT 
AAAGAAGAGG GTGTGCGGGA CGTCCTTAAG GATGTACTGC TCCCATGGTA CAATGCCTAT 
CGCTTCTTAA TCCAGAACGT TCTGAGGCTC CAGAAGGAGG AAGAAATAGA ATTTCTCTAC 
AATGAGAACA CGGTTAGAGA AAGCCCCAAC ATTACAGACC GGTGGATCCT GTCCTTCATG 
CAGTCTCTCA TTGGCTTCTT TGAGACTGAA ATGGCAGCTT ATAGGCTTTA TACTGTGGTG 
CCTCGCCTGG TCAAGTTTGT AGATATTCTG ACCAATTGGT ATGTTAGAAT GAACCGCAGA 
AGATTAAAGG GTGAAAATGG GATGGAGGAT TGTGTCATGG CCCTAGAAAC CTTGTTTAGT 
GTTCTGCTTT CTCTTTGCAG ACTTATGGCT CCCTACACAC CTTTTCTCAC TGAATTGATG 
TACCAGAATC TAAAGGTGCT GATTGACCCT GTTTCTGTTC AGGACAAGGA CACACTCAGC 
ATTCACTACC TCATGCTGCC CCGTGTTCGA GAAGAATTGA TTGACAAGAA AACAGAGAGT 
GCAGTATCTC AGATGCAGTC TGTGATTGAA CTTGGAAGAG TGATCAGAGA CCGAAAAACT 
ATTCCCATAA AGTATCCTTT GAAAGAAATT GTGGTTATCC ATCAAGATCC AGAAGCTCTT 
AAAGATATCA AGTCTTTGGA GAAGTATATC ATTGAGGAAC TCAATGTTCG AAAAGTTACA 
CTGTCTACAG ATAAAAACAA GTATGGCATT CGGCTAAGGG CAGAACCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAGGG AGCCTTTAAG GCAGTGATGA CGTCCATCAA GCAGTTGAGC 
AGTGAGGAGC TGGAGCAGTT CCAGAAGACT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CACGATGAAG ACATCCGCCT CATGTACACC TTTGATCAGG CCACAGGTGG GACTGCGCAA 
TTTGAAGCAC ACTCAGATGC TCAGGCTTTG GTCCTCTTAG ATGTCACTCC TGACCAGTCA 
ATGGTAGATG AAGGAATGGC TCGGGAAGTC ATCAATCGCA TACAGAAACT TCGCAAAAAG 
TGCAATCTGG TTCCAACTGA TGAAATCACA GTGTACTATA AAGCAAAGTC TGAAGGAACA 
TATCTGAATA GTGTTATTGA AAGCCACACA GAGTTCATAT TTACCACCAT AAAGGCTCCC 
TTGAAACCAT ATCCAGTTTC TCCATCGGAT AAAGTCCTTA TTCAAGAAAA AACACAGTTG 
AAGGGATCTG AACTGGAAAT TACACTCACC AGAGGATCTT CCCTTCCTGG TCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTGCAAAT GGCAGTGAAC AAGGTGGAGT ATTGCTCCTG 
GAAAATCCAA AAGGTGACAA TAGGTTGGAC CTTTTAAAGC TGAAGAGTGT TGTCACTAGC 
ATTTTTGGTG TGAAAAATAC AGAGCTGGCT GTCTTCCATG ATGAAACAGA AATACAAAAC 
CAAACTGACT TACTGAGTCT TAGTGGAAAA ACACTTTGTG TGACTGCAGG ATCGGCTCCC 
TCTCTGATCA ACAGTTCTAG TACTCTTCTT TGTCAGTATA TCAACCTACA GCTCCTGAAT 
GCAAAGCCAC AAGAGTGTTT AATGGGGACA GTGGGCACTC TCCTGCTTGA AAACCCACTT 
GGGCAGAATG GACTCACCCA CCAAGGTCTT CTGTATGAAG CAGCCAAGGT GTTTGGCCTT 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACCCAAA CGCAGGAAAT TACAGAAGAC 
ATCCCCGTGA AGACTTTGAA TATGAAGACT GTGTATGTTT CTGTGTTACC AACAACAGCA 
GACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

New evidence for association of recessive IARS gene mutations with hepatopathy, hypotonia, intellectual disability and growth retardation.
Clinical genetics92(6)671-673(2017 Dec)
Smigiel R,Biela M,Biernacka A,Stembalska A,Sasiadek M,Kosinska J,Rydzanicz M,Ploski R

Bi-allelic IARS mutations in a child with intra-uterine growth retardation, neonatal cholestasis, and mild developmental delay.
Clinical genetics91(6)913-917(2017 Jun)
Orenstein N,Weiss K,Oprescu SN,Shapira R,Kidron D,Vanagaite-Basel L,Antonellis A,Muenke M

Biallelic IARS Mutations Cause Growth Retardation with Prenatal Onset, Intellectual Disability, Muscular Hypotonia, and Infantile Hepatopathy.
American journal of human genetics99(2)414-22(2016 Aug)
Kopajtich R,Murayama K,Janecke AR,Haack TB,Breuer M,Knisely AS,Harting I,Ohashi T,Okazaki Y,Watanabe D,Tokuzawa Y,Kotzaeridou U,K?lker S,Sauer S,Carl M,Straub S,Entenmann A,Gizewski E,Feichtinger RG,Mayr JA,Lackner K,Strom TM,Meitinger T,M?ller T,Ohtake A,Hoffmann GF,Prokisch H,Staufner C

Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton.
Journal of immunology (Baltimore, Md. : 1950)192(2)771-81(2014 Jan)
Forster F,Paster W,Supper V,Schatzlmaier P,Sunzenauer S,Ostler N,Saliba A,Eckerstorfer P,Britzen-Laurent N,Sch?tz G,Schmid JA,Zlabinger GJ,Naschberger E,St?rzl M,Stockinger H

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IARS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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