IleRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	IleRS
Entrez Gene ID	45785
Full Name	Isoleucyl-tRNA synthetase
Synonyms	Aats-ile,CG11471,Dmel\CG11471,IRS,l(3)00827
General protein information	Preferred Names Isoleucyl-tRNA synthetase Names CG11471 gene product from transcript CG11471-RA CG11471-PA CG11471-PC CG11471-PD IleRS-PA IleRS-PC IleRS-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 79D4-79D4

mRNA and Protein(s)

mRNA	Protein	Name
NM_168952.4	NP_730716.1	Isoleucyl-tRNA synthetase, isoform A
NM_080101.4	NP_524840.2	Isoleucyl-tRNA synthetase, isoform C
NM_176387.2	NP_788565.1	Isoleucyl-tRNA synthetase, isoform D

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IleRS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IleRS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19418	NM_176387.2 Latest version!	Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa19418	NM_080101.4 Latest version!	Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa19418	NM_168952.4 Latest version!	Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IleRS ( NM_168952.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730716.1 Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa19418
Clone ID Related Accession (Same CDS sequence)	NM_168952.4 , NM_080101.4 , NM_176387.2
Accession Version	NM_168952.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Isoleucyl-tRNA synthetase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168952.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC 
CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG 
CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA 
TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG 
GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA 
ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG 
AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC 
CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC 
TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC 
GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC 
GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA 
AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC 
GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG 
TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT 
TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG 
TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC 
GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA 
GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA 
GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC 
CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG 
GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC 
CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG 
GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG 
TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC 
GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA 
GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA 
GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC 
AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA 
CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT 
GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT 
CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG 
CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA 
GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT 
GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG 
AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC 
GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA 
AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT 
GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA 
ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG 
GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG 
CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA 
TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT 
GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG 
CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG 
AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG 
GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG 
CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC 
ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG 
GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT 
GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC 
GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT 
CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG 
TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG 
CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG 
CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA 
AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG 
CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT 
TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC 
GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA 
CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG 
GAAAACCCCT TGGGACGCGC CTTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730716.1
CDS	133..3822
Translation	MGKKLERNDVCRVPENINFPAEEENVLQKWRHENIFEKCSQLSKGKPKYTFYDGPPFATGLPHYGHILAGTIKDIVTRYAYQQGYHVDRRFGWDCHGLPVEFEIDKLLNIKGPEDVAKMGIAAYNAECRKIVMRYADEWENVVTRVGRWIDFKNDYKTLYPWYMESIWWIFKQLFDKGLVYQGVKVMPYSTACTTSLSNFEANQNYKEVVDPCVVVALEAVSLPNTFFLVWTTTPWTLPSNFACCVHPTMTYVKVRDVKSDRLFVLAESRLSYVYKSETEYEVKEKFVGKTLKDLHYKPLFPYFAKRGAEVKAYRVLVDEYVTEDSGTGIVHNAPYFGEDDYRVCLAAGLITKSSEVLCPVDEAGRFTNEASDFEGQYVKDSDKQIMAALKARGNLVSSGQVKHSYPFCWRSDTPLIYKAVPSWFVRVEHMSKNLLDCSSQTYWVPDFVKEKRFGNWLKEARDWAISRNRYWGTPIPIWRSPSGDETVVIGSIKQLAELSGVQVEDLHRESIDHIEIPSAVPGNPPLRRIAPVFDCWFESGSMPFAQQHFPFENEKDFMNNFPADFIAEGIDQTRGWFYTLLVISTALFNKAPFKNLIASGLVLAADGQKMSKRKKNYPDPMEVVHKYGADALRLYLINSPVVRAESLRFKEEGVRDIIKDVFLPWYNAYRFLLQNIVRYEKEDLAGNGQYTYDRERHLKNMDKASVIDVWILSFKESLLEFFATEMKMYRLYTVVPRLTKFIDQLTNWYVRLNRRRIKGELGADQCIQSLDTLYDVLYTMVKMMAPFTPYLTEYIFQRLVLFQPAGTLEHADSVHYQMMPVSQKKFIRNDIERSVALMQSVVELGRVMRDRRTLPVKYPVSEIIAIHKDSQILEAIKTLQDFILSELNVRKLTLSSDKEKYGVTLRAEPDHKALGQRLKGNFKAVMAAIKALRDDEIQKQVSQGYFDILDQRIELDEVRIIYCTSEQVGGNFEAHSDNEVLVLLDMTPNEELLEEGLAREVINRVQKLKKKAQLIPTDPVLIFHELAADNKAKQEVLEAQAQLAKVLSNYASIIKTAIKSEFAPYSSEQASKKRLIASELVDLKGVPLKLTICSTEELQLPNLPWLNISLAEDLVPRFGNSSKASLFLQHNVSKEIISLPTLRSELEHLFGLYGVNFNIYVVDHQKRTTALKSIDENLSGKLLVLTRSQDAPKLSAGYELSPAPYSKFINQHSGKSIFTENPLGRALC

Target ORF information:

RefSeq Version	NM_168952.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168952.4

ORF Insert Sequence:

ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC 
CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG 
CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA 
TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG 
GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA 
ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG 
AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC 
CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC 
TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC 
GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC 
GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA 
AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC 
GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG 
TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT 
TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG 
TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC 
GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA 
GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA 
GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC 
CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG 
GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC 
CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG 
GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG 
TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC 
GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA 
GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA 
GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC 
AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA 
CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT 
GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT 
CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG 
CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA 
GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT 
GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG 
AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC 
GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA 
AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT 
GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA 
ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG 
GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG 
CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA 
TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT 
GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG 
CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG 
AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG 
GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG 
CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC 
ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG 
GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT 
GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC 
GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT 
CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG 
TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG 
CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG 
CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA 
AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG 
CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT 
TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC 
GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA 
CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG 
GAAAACCCCT TGGGACGCGC CTTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IleRS ( NM_080101.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524840.2 Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa19418
Clone ID Related Accession (Same CDS sequence)	NM_168952.4 , NM_080101.4 , NM_176387.2
Accession Version	NM_080101.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Isoleucyl-tRNA synthetase, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_080101.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC 
CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG 
CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA 
TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG 
GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA 
ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG 
AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC 
CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC 
TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC 
GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC 
GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA 
AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC 
GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG 
TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT 
TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG 
TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC 
GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA 
GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA 
GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC 
CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG 
GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC 
CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG 
GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG 
TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC 
GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA 
GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA 
GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC 
AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA 
CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT 
GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT 
CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG 
CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA 
GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT 
GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG 
AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC 
GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA 
AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT 
GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA 
ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG 
GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG 
CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA 
TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT 
GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG 
CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG 
AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG 
GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG 
CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC 
ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG 
GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT 
GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC 
GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT 
CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG 
TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG 
CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG 
CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA 
AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG 
CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT 
TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC 
GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA 
CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG 
GAAAACCCCT TGGGACGCGC CTTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524840.2
CDS	54..3743
Translation	MGKKLERNDVCRVPENINFPAEEENVLQKWRHENIFEKCSQLSKGKPKYTFYDGPPFATGLPHYGHILAGTIKDIVTRYAYQQGYHVDRRFGWDCHGLPVEFEIDKLLNIKGPEDVAKMGIAAYNAECRKIVMRYADEWENVVTRVGRWIDFKNDYKTLYPWYMESIWWIFKQLFDKGLVYQGVKVMPYSTACTTSLSNFEANQNYKEVVDPCVVVALEAVSLPNTFFLVWTTTPWTLPSNFACCVHPTMTYVKVRDVKSDRLFVLAESRLSYVYKSETEYEVKEKFVGKTLKDLHYKPLFPYFAKRGAEVKAYRVLVDEYVTEDSGTGIVHNAPYFGEDDYRVCLAAGLITKSSEVLCPVDEAGRFTNEASDFEGQYVKDSDKQIMAALKARGNLVSSGQVKHSYPFCWRSDTPLIYKAVPSWFVRVEHMSKNLLDCSSQTYWVPDFVKEKRFGNWLKEARDWAISRNRYWGTPIPIWRSPSGDETVVIGSIKQLAELSGVQVEDLHRESIDHIEIPSAVPGNPPLRRIAPVFDCWFESGSMPFAQQHFPFENEKDFMNNFPADFIAEGIDQTRGWFYTLLVISTALFNKAPFKNLIASGLVLAADGQKMSKRKKNYPDPMEVVHKYGADALRLYLINSPVVRAESLRFKEEGVRDIIKDVFLPWYNAYRFLLQNIVRYEKEDLAGNGQYTYDRERHLKNMDKASVIDVWILSFKESLLEFFATEMKMYRLYTVVPRLTKFIDQLTNWYVRLNRRRIKGELGADQCIQSLDTLYDVLYTMVKMMAPFTPYLTEYIFQRLVLFQPAGTLEHADSVHYQMMPVSQKKFIRNDIERSVALMQSVVELGRVMRDRRTLPVKYPVSEIIAIHKDSQILEAIKTLQDFILSELNVRKLTLSSDKEKYGVTLRAEPDHKALGQRLKGNFKAVMAAIKALRDDEIQKQVSQGYFDILDQRIELDEVRIIYCTSEQVGGNFEAHSDNEVLVLLDMTPNEELLEEGLAREVINRVQKLKKKAQLIPTDPVLIFHELAADNKAKQEVLEAQAQLAKVLSNYASIIKTAIKSEFAPYSSEQASKKRLIASELVDLKGVPLKLTICSTEELQLPNLPWLNISLAEDLVPRFGNSSKASLFLQHNVSKEIISLPTLRSELEHLFGLYGVNFNIYVVDHQKRTTALKSIDENLSGKLLVLTRSQDAPKLSAGYELSPAPYSKFINQHSGKSIFTENPLGRALC

Target ORF information:

RefSeq Version	NM_080101.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080101.4

ORF Insert Sequence:

ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC 
CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG 
CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA 
TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG 
GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA 
ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG 
AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC 
CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC 
TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC 
GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC 
GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA 
AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC 
GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG 
TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT 
TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG 
TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC 
GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA 
GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA 
GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC 
CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG 
GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC 
CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG 
GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG 
TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC 
GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA 
GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA 
GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC 
AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA 
CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT 
GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT 
CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG 
CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA 
GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT 
GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG 
AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC 
GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA 
AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT 
GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA 
ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG 
GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG 
CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA 
TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT 
GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG 
CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG 
AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG 
GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG 
CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC 
ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG 
GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT 
GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC 
GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT 
CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG 
TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG 
CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG 
CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA 
AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG 
CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT 
TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC 
GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA 
CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG 
GAAAACCCCT TGGGACGCGC CTTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IleRS ( NM_176387.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788565.1 Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant D, mRNA.

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CloneID	OFa19418
Clone ID Related Accession (Same CDS sequence)	NM_168952.4 , NM_080101.4 , NM_176387.2
Accession Version	NM_176387.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Isoleucyl-tRNA synthetase, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_176387.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC 
CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG 
CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA 
TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG 
GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA 
ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG 
AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC 
CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC 
TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC 
GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC 
GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA 
AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC 
GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG 
TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT 
TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG 
TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC 
GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA 
GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA 
GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC 
CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG 
GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC 
CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG 
GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG 
TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC 
GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA 
GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA 
GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC 
AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA 
CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT 
GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT 
CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG 
CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA 
GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT 
GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG 
AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC 
GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA 
AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT 
GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA 
ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG 
GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG 
CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA 
TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT 
GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG 
CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG 
AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG 
GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG 
CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC 
ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG 
GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT 
GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC 
GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT 
CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG 
TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG 
CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG 
CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA 
AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG 
CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT 
TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC 
GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA 
CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG 
GAAAACCCCT TGGGACGCGC CTTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788565.1
CDS	79..3768
Translation	MGKKLERNDVCRVPENINFPAEEENVLQKWRHENIFEKCSQLSKGKPKYTFYDGPPFATGLPHYGHILAGTIKDIVTRYAYQQGYHVDRRFGWDCHGLPVEFEIDKLLNIKGPEDVAKMGIAAYNAECRKIVMRYADEWENVVTRVGRWIDFKNDYKTLYPWYMESIWWIFKQLFDKGLVYQGVKVMPYSTACTTSLSNFEANQNYKEVVDPCVVVALEAVSLPNTFFLVWTTTPWTLPSNFACCVHPTMTYVKVRDVKSDRLFVLAESRLSYVYKSETEYEVKEKFVGKTLKDLHYKPLFPYFAKRGAEVKAYRVLVDEYVTEDSGTGIVHNAPYFGEDDYRVCLAAGLITKSSEVLCPVDEAGRFTNEASDFEGQYVKDSDKQIMAALKARGNLVSSGQVKHSYPFCWRSDTPLIYKAVPSWFVRVEHMSKNLLDCSSQTYWVPDFVKEKRFGNWLKEARDWAISRNRYWGTPIPIWRSPSGDETVVIGSIKQLAELSGVQVEDLHRESIDHIEIPSAVPGNPPLRRIAPVFDCWFESGSMPFAQQHFPFENEKDFMNNFPADFIAEGIDQTRGWFYTLLVISTALFNKAPFKNLIASGLVLAADGQKMSKRKKNYPDPMEVVHKYGADALRLYLINSPVVRAESLRFKEEGVRDIIKDVFLPWYNAYRFLLQNIVRYEKEDLAGNGQYTYDRERHLKNMDKASVIDVWILSFKESLLEFFATEMKMYRLYTVVPRLTKFIDQLTNWYVRLNRRRIKGELGADQCIQSLDTLYDVLYTMVKMMAPFTPYLTEYIFQRLVLFQPAGTLEHADSVHYQMMPVSQKKFIRNDIERSVALMQSVVELGRVMRDRRTLPVKYPVSEIIAIHKDSQILEAIKTLQDFILSELNVRKLTLSSDKEKYGVTLRAEPDHKALGQRLKGNFKAVMAAIKALRDDEIQKQVSQGYFDILDQRIELDEVRIIYCTSEQVGGNFEAHSDNEVLVLLDMTPNEELLEEGLAREVINRVQKLKKKAQLIPTDPVLIFHELAADNKAKQEVLEAQAQLAKVLSNYASIIKTAIKSEFAPYSSEQASKKRLIASELVDLKGVPLKLTICSTEELQLPNLPWLNISLAEDLVPRFGNSSKASLFLQHNVSKEIISLPTLRSELEHLFGLYGVNFNIYVVDHQKRTTALKSIDENLSGKLLVLTRSQDAPKLSAGYELSPAPYSKFINQHSGKSIFTENPLGRALC

Target ORF information:

RefSeq Version	NM_176387.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Isoleucyl-tRNA synthetase (IleRS), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176387.2

ORF Insert Sequence:

ATGGGAAAAA AACTAGAGCG GAACGACGTG TGTCGGGTGC CAGAGAACAT TAATTTCCCG 
GCCGAGGAGG AGAACGTACT GCAGAAATGG CGACACGAAA ATATCTTTGA AAAGTGCAGC 
CAGCTGTCGA AGGGAAAGCC CAAATACACG TTCTACGATG GACCGCCCTT TGCAACCGGG 
CTGCCCCACT ACGGACACAT TCTTGCTGGC ACCATCAAGG ACATCGTCAC ACGGTACGCA 
TACCAGCAGG GATACCATGT GGACCGTCGC TTCGGCTGGG ATTGCCACGG ACTGCCCGTG 
GAGTTCGAGA TTGACAAGCT GCTGAATATC AAGGGTCCTG AGGACGTGGC CAAGATGGGA 
ATCGCAGCCT ACAATGCCGA GTGCCGGAAG ATTGTCATGC GTTACGCAGA TGAGTGGGAG 
AATGTGGTGA CGCGCGTGGG TCGCTGGATT GACTTCAAGA ACGATTACAA GACGTTGTAC 
CCATGGTACA TGGAGTCCAT TTGGTGGATC TTCAAGCAAC TGTTCGACAA GGGTCTGGTC 
TACCAGGGCG TTAAGGTGAT GCCCTATTCC ACCGCCTGCA CAACGTCCCT TTCGAACTTC 
GAGGCCAACC AAAACTACAA GGAGGTTGTG GACCCTTGCG TAGTAGTTGC ACTGGAGGCC 
GTTTCGTTGC CCAATACCTT CTTCCTAGTG TGGACCACCA CGCCATGGAC ACTGCCCTCA 
AACTTCGCAT GCTGTGTTCA TCCGACGATG ACCTATGTAA AAGTGCGCGA CGTAAAGAGC 
GACCGACTTT TTGTTCTCGC AGAGTCTCGT CTCTCTTACG TGTACAAATC GGAGACGGAG 
TACGAAGTGA AGGAAAAGTT TGTTGGCAAA ACCCTTAAGG ACCTTCACTA CAAGCCGCTT 
TTCCCTTACT TTGCGAAGCG AGGAGCTGAG GTGAAAGCTT ATCGTGTCCT GGTCGACGAG 
TACGTGACTG AGGATTCCGG TACGGGTATT GTGCACAATG CTCCCTACTT CGGAGAAGAC 
GATTATCGTG TGTGCCTCGC TGCGGGCCTC ATCACAAAGT CCAGTGAGGT TCTCTGCCCA 
GTGGACGAGG CAGGACGGTT TACTAACGAG GCAAGCGATT TCGAGGGCCA ATACGTCAAA 
GATTCTGATA AGCAAATAAT GGCCGCGCTG AAGGCACGAG GAAACTTGGT TTCCAGTGGC 
CAGGTGAAGC ATAGCTACCC ATTCTGCTGG CGGTCAGACA CGCCTCTTAT CTATAAGGCG 
GTTCCTTCCT GGTTTGTGCG CGTCGAGCAC ATGTCTAAAA ACCTGCTTGA TTGCAGTTCC 
CAAACGTACT GGGTGCCGGA CTTCGTCAAG GAGAAACGTT TTGGAAACTG GCTGAAAGAG 
GCCAGAGATT GGGCTATCTC CCGCAATCGC TACTGGGGCA CGCCGATTCC TATCTGGAGG 
TCGCCTAGCG GTGATGAGAC CGTCGTTATC GGCAGTATCA AGCAGTTAGC CGAGCTTTCC 
GGCGTTCAAG TCGAGGATCT TCATAGAGAA AGTATCGACC ACATTGAAAT ACCATCGGCA 
GTTCCTGGAA ATCCACCACT GCGTCGCATA GCTCCTGTTT TTGACTGTTG GTTTGAATCA 
GGTTCAATGC CGTTCGCACA ACAGCATTTC CCTTTTGAAA ACGAAAAGGA CTTTATGAAC 
AATTTCCCGG CTGACTTTAT AGCAGAGGGT ATCGATCAGA CCCGAGGCTG GTTTTACACA 
CTCCTTGTGA TTTCGACGGC GCTATTTAAT AAGGCACCAT TTAAAAACCT CATTGCAAGT 
GGACTGGTCT TAGCTGCTGA TGGTCAAAAA ATGTCGAAGC GCAAGAAGAA CTATCCCGAT 
CCAATGGAGG TGGTCCATAA GTATGGAGCG GATGCTCTCC GACTGTACCT TATCAATTCG 
CCCGTCGTAA GGGCCGAGAG TCTGCGTTTT AAGGAAGAAG GTGTGCGCGA CATAATCAAA 
GATGTGTTCC TACCGTGGTA CAATGCATAC CGATTCTTAC TACAAAATAT CGTTCGATAT 
GAGAAGGAGG ATTTGGCAGG AAATGGACAA TATACTTATG ATCGTGAACG CCACTTAAAG 
AATATGGACA AGGCCTCCGT TATAGATGTG TGGATTTTGT CTTTTAAGGA ATCCTTACTC 
GAGTTCTTTG CCACTGAGAT GAAAATGTAT CGCCTGTACA CTGTCGTACC CCGGCTGACA 
AAGTTCATTG ATCAACTTAC CAATTGGTAT GTTCGACTAA ATCGACGTCG CATCAAGGGT 
GAATTGGGAG CTGATCAGTG CATTCAATCA CTTGACACGC TTTACGATGT TCTATACACA 
ATGGTTAAAA TGATGGCTCC TTTCACGCCT TACCTTACGG AGTACATATT CCAGCGGCTG 
GTATTGTTCC AGCCAGCCGG AACCCTAGAG CATGCTGACT CAGTTCACTA CCAGATGATG 
CCGGTCAGTC AGAAAAAATT TATTCGAAAC GATATTGAGC GCTCGGTGGC TTTGATGCAA 
TCAGTTGTCG AGCTGGGTCG TGTAATGCGG GATCGAAGAA CCTTGCCTGT TAAGTATCCT 
GTTTCGGAGA TCATAGCAAT TCACAAGGAC TCGCAAATCC TGGAGGCAAT CAAGACCTTG 
CAGGACTTCA TTCTTAGCGA ACTCAATGTT CGAAAACTTA CGCTAAGCTC TGATAAGGAG 
AAATATGGTG TAACATTGAG AGCAGAGCCT GACCACAAGG CGCTAGGTCA GCGGCTTAAG 
GGCAATTTCA AAGCGGTTAT GGCTGCTATC AAGGCTCTCA GGGATGATGA AATTCAGAAG 
CAAGTTTCGC AGGGTTACTT TGACATTCTG GATCAAAGAA TTGAACTTGA TGAAGTTCGC 
ATCATTTACT GCACATCTGA GCAGGTTGGA GGAAACTTTG AGGCTCACAG CGATAATGAG 
GTTTTGGTAC TGTTGGACAT GACGCCTAAC GAAGAATTGC TTGAAGAGGG CTTGGCTCGT 
GAGGTCATAA ATAGAGTCCA GAAACTAAAA AAGAAGGCTC AACTTATACC GACCGATCCC 
GTTCTTATCT TCCATGAGCT AGCTGCCGAT AACAAAGCTA AGCAGGAGGT CCTGGAGGCT 
CAGGCTCAGC TAGCCAAAGT TTTGTCCAAC TATGCCAGCA TAATCAAGAC GGCCATTAAG 
TCGGAGTTTG CTCCCTATTC ATCTGAGCAG GCGTCCAAGA AGCGTTTAAT AGCTAGTGAG 
CTTGTCGATC TTAAAGGAGT TCCCTTAAAG TTGACTATTT GTTCGACTGA GGAATTACAG 
CTTCCTAATT TGCCTTGGTT AAATATATCC TTGGCAGAAG ATCTGGTGCC ACGATTTGGA 
AATAGCAGCA AAGCGTCTCT ATTTCTGCAG CACAACGTTA GCAAGGAAAT TATTTCACTG 
CCTACACTTC GCTCTGAGTT GGAGCACTTG TTTGGATTGT ATGGAGTTAA CTTTAATATT 
TACGTTGTTG ATCACCAAAA AAGGACTACT GCACTAAAGT CTATCGATGA AAACCTAAGC 
GGAAAATTGC TTGTTCTAAC TCGAAGCCAA GATGCACCGA AACTATCAGC AGGCTATGAA 
CTATCCCCCG CTCCTTACTC CAAATTCATC AACCAGCATT CAGGAAAATC TATCTTCACG 
GAAAACCCCT TGGGACGCGC CTTATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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IleRS cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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