iars1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	iars1
Entrez Gene ID	334393
Full Name	isoleucyl-tRNA synthetase
Synonyms	fi46h05,wu:fi46h05,zgc:63790
General protein information	Preferred Names isoleucyl-tRNA synthetase Names isoleucine--tRNA ligase, cytoplasmic
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_199896.2	NP_956190.2	isoleucine--tRNA ligase, cytoplasmic

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases.
Waldron AL, Cahan SH, Franklyn CS, Ebert AM
PloS one12(9)e0185317(2017)

Biallelic IARS Mutations Cause Growth Retardation with Prenatal Onset, Intellectual Disability, Muscular Hypotonia, and Infantile Hepatopathy.
Kopajtich R, Murayama K, Janecke AR, Haack TB, Breuer M, Knisely AS, Harting I, Ohashi T, Okazaki Y, Watanabe D, Tokuzawa Y, Kotzaeridou U, K?lker S, Sauer S, Carl M, Straub S, Entenmann A, Gizewski E, Feichtinger RG, Mayr JA, Lackner K, Strom TM, Meitinger T, M?ller T, Ohtake A, Hoffmann GF, Prokisch H, Staufner C
American journal of human genetics99(2)414-22(2016 Aug)

Aminoacyl-Transfer RNA Synthetase Deficiency Promotes Angiogenesis via the Unfolded Protein Response Pathway.
Castranova D, Davis AE, Lo BD, Miller MF, Paukstelis PJ, Swift MR, Pham VN, Torres-V?zquez J, Bell K, Shaw KM, Kamei M, Weinstein BM
Arteriosclerosis, thrombosis, and vascular biology36(4)655-62(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in isoleucyl tRNA synthetase (iars(y68)) leads to increased branching angiogenesis in developing zebrafish.
Title: Aminoacyl-Transfer RNA Synthetase Deficiency Promotes Angiogenesis via the Unfolded Protein Response Pathway.

The following iars1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the iars1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa53215	NM_199896.2 Latest version!	Danio rerio isoleucyl-tRNA synthetase 1 (iars1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

iars1 ( NM_199896.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956190.2 Danio rerio isoleucyl-tRNA synthetase 1 (iars1), mRNA.

Price & Availability↑

CloneID	ODa53215
Clone ID Related Accession (Same CDS sequence)	NM_199896.2
Accession Version	NM_199896.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-28
Organism	Danio rerio(zebrafish)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01073876.1, CABZ01073875.1, CABZ01073874.1, EH583791.1, CABZ01073873.1, CABZ01073872.1, CABZ01073871.1, CABZ01073870.1, CABZ01073869.1, CABZ01073868.1, EH601215.1, CABZ01073867.1 and CABZ01073866.1. On Oct 21, 2015 this sequence version replaced NM_199896.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC056826.1, GDQH01008029.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGAAG CAGTGCCAGA GTCCATCCAC TTCCCCACTG AGGAGGAGCA AATCCTGCAG 
TTCTGGCAGA AGAACGACTG TTTCCAGGAG TGTCTGAAGC AGTCAAAGAG CCGGCCCAGG 
TTCACGTTCT ACGACGGGCC GCCCTTCGCC ACAGGTCTGC CTCACTATGG TCATATTCTG 
GCGGGAACCA TCAAGGACAT CGTGACGCGA TTCGCTCATC AGAACGGCTT CCACGTGGAG 
CGGCGCTTCG GCTGGGACTG CCATGGCCTG CCGGTGGAGT ATGAGATCGA TAAATCTCTG 
AACATCAAAG GACCCGAGGA TGTGATGAAG ATGGGCATCG AGGAGTACAA CAAACAGTGC 
CGGAGCATCG TCATGAGATA TGCTGATGAG TGGGAGAACT CGGTGAGGCG GATGGGGCGC 
TGGATTGACT TCAGGAACGA CTATAAGACC CTCTACCCCT GGTTCATGGA GACTGTGTGG 
TGGGTGTTTA AGCAGCTGTA TGACAGTGGT CTGGTGTACC GCGGGGTGAA GGTGATGCCG 
TTCTCCACCG CCTGCAACAC TCCGCTCTCC AACTTTGAGG CCAATCAGAA CTACAAGGAT 
GTGCAGGACC CGTCAGTGAT CGTCAATTTC CCGTTGCTGG AGGATCCGTC TGTCAGCTTG 
ATCGCCTGGA CCACCACACC CTGGACCCTC CCCAGCAACC TGGCACTCTG CGTCAACCCA 
GAGCTCATCT ACGTCCGGGT CAAAGACAAC AGCTCACAGA AGATCTACAT CATGATGGAG 
GCCAGACTCG TCGCTCTCTT CAAGTCTGAC TCTGAATACA CCATTCTGGA GCGATTTCCT 
GGCAAGACTC TGAAGGGCAA AAAATACAAG CCTCTTTTCG AGTACTTCAT CAAGTGTAAG 
GAGACCGGTG CCTTCTCGGT AGTGATGGAT AATTATGTTC GTGATGATGA CGGTACAGGA 
GTCGTCCATC AGGCCCCGTA CTTCGGAGCT GATGATTATC GTGTGTGTAT GGAGTATGAC 
ATCATCCAGA GAGACTCCGC CCCCATCTGC CCGGTCGACC CATCTGGCAT ATTCACTCCG 
GAGGTCACAC ACTTCGCCGG ACAGTATGTG AAGGATGCAG ACAAAAACAT CATCAAGTGG 
CTGAAGGAGA ACGGACGTCT GGTGAGCGCC AGCTCCTTCA AGCACAGCTA CCCGTTCTGC 
TGGAGGTCGG ACACGCCGCT GATCTACAAA GCAGTGCCCA GCTGGTTTAT AAGGGTGGAG 
CACATGGTGG AGAAACTTCT GGACAACAAC AGCAAGTGCT ACTGGGTGCC AGAGTTTGTG 
AAGGAGAAGC GTTTCGGGAA CTGGCTGAGG GACGCCAGGG ACTGGGCGGT GTCCCGCAAC 
CGCTACTGGG GCACACCTAT CCCTCTGTGG GTCAGTGACG ACTTCGAGGA GGTGGTGTGT 
GTGGGATCCA TGGCGGAGCT GGAGGAGCTG ACGGGAATTA AAGTCACTGA TCTCCACCGA 
GAAAGCATTG ACGGCCTCAC CATCCCCTCT CGCTGTGGGA AAGGGCAGCT CCGGCGGGTT 
TCGGAGGTGT TCGACTGCTG GTTCGAGAGC GGCAGTATGC CCTACGCTCA GGTGCACTAC 
CCGTTTGAGA ACAGGCGCGA GTTCGAAGAC GCATTCCCTG CAGACTTCAT CGCAGAGGGC 
ATCGACCAGA CCAGAGGATG GTTCTACACG TTACTCGTGC TCTCGACGGC TCTGTTTGGG 
AAACCGCCAT TCAAGAACGT GATCGTCAAC GGTTTGGTTT TGGCCAGTGA TGGGCAGAAA 
ATGAGCAAGC GGAAGAAGAA TTACCCCGAC CCCGGACTCA TCGTGCAGAG CTACGGTGCA 
GACGCACTAC GGCTGTATCT GATCAACTCT CCTGTGGTTC GAGCTGAAAA CCTGCGCTTC 
AAAGAGGAAG GAGTTCGAGA TGTGCTGAAA GACGTCTTCC TACCCTGGTA CAATGCATAC 
CGGTTCCTGG TGCAGAACGT CCAGAGACTG CAGAAGGAGG AAGGCGTTGA GTTCCTGTAC 
AACGAGAGGA CGTCCAGCGT CAGCAACAAC ATCATGGATA AATGGATCCA GTCTTTCACA 
CAGTCACTCA TTCAGTTCTT CCGGGACGAG ATGGGAGCCT ACAGACTCTA TACTGTGGTG 
CCCAAGCTTG TCAAGTTTGT GGATATGCTC ACTAACTGGT ACGTGCGGAT GAACCGCCGG 
CGGCTGAAGG GTGAAAGTGG TTCTGAGGAC TGCGTTCGTG CTCTGGAAAC CCTCTTCAGT 
GTCCTGTTCT CCATGTGCAG ACTGATGGCT CCGTTCACTC CGTTTATAAC CGAGCTGATG 
TACCAGAACC TGCGGCACCT GCTGGACCCG GCAGCAGTGG AGGAGAAGGA CACCGGCAGC 
ATACACTACC TCATGCTGCC ACAAGTCAGG GAGTCTCTGA TTGACCAGCG TATTGAGAGT 
GCGGTGTCTC GGATGCAGTC GGTCATTGAG TTGGGTCGTG TAATCAGAGA CAGGAAGACT 
CTGCCCATCA AGTATCCGCT GAAGGAGGTG GTGGTCATCC ATCAGGACCC AGAGGCTCTC 
ACTGATATCC AGTCCCTGCA GAACTACATC CTGGAGGAGC TGAACGTGCG CCAGCTCACT 
CTCTCCACTG ATAAGGACCG ATACGGCATC CGTCTGCGGG CCGAACCCGA TCACATGGTG 
CTGGGCAAAC GGCTGAAAGG AGCCTTTAAA TCCGTGACCG CGTCCATCAA AGAGCTGAAG 
AGTGAAGAGC TGGAGGAGTT CCAGAAGACA GGTTGTATAG TGGTGGATGG TCATGACCTG 
CGTGAGGAGG ATCTGCGTCT TATGTACACC TTCGATCAGT CGTCTGGATC CGCGTCTCAG 
TACGAGGCTC ACTCAGACGC TCAGGTGCTG GTTCTGCTGG ACGTCACGCC GGATCAGGCC 
ATGCTGGACG AAGGAGTGGC CCGAGAGGTC ATCAACCGCA TCCAGAAACT GCGTAAGAAG 
GGTCACCTGG TGCCGTCAGA TGAGATCACG GTGTTCTACC GCTGTCAGCC GGCTGGTGAA 
TATCTGCATC AGGTGATTGA GGCACACACA GACTTCATCC TGGCCACCAC TAAAGCTCCG 
CTCAGAGCGT ACCCCGTGTG TCCCAACGCC AGCGTCATCG TGCAGGAGAA CACACAGCTG 
AAGGGCTCAG ATCTGGAGTT GACTATCGTG CGAGGCTCCT CCTCTTCCTC CGTCTGCAGG 
GCTCCTGTGA GCGGCTCGGC GTGTGCGTTT GTGAACCTCA GAGTGTCGCT CAAAGATAAA 
CAGCAGGACG GTGTGGTTCT GCTGGAGAAC CCCAAGGGAG ACAACAGACT GGACCTGGAG 
CAGCTGAAGA GGATCTGCTG CAGTGTGTTC GGCCTCAACG GAGCTCCAGT CTCCATATTC 
AACGGCAAGA CAGCACTCAG CAGCAGCACT GATCTGCTGT CTCTGAGCGG CAGGACTCTG 
ACTGTGAGCT CGGCTGCGGG TTCGAGTCCT GCAGATGTGA GCAGCTCCGA GTCTCTGCTG 
TGTCCGTATG TGAACCTGCA GCTCGTCAAC GCCAGTCCTG CAGAGTGTCG GACTGGAGAC 
GTGGCGACGC TGCTCCTGAT GAACCCTGAG GGTAAAAACC TCCTCCAGTT CTCAGATCTG 
CTGAGAGAAA CGGCTAAAGT GTTCGGTCTG AGAGGAAGAA GACTGATGCT CTACACTGAT 
CCACAGCTCA CTGCAGAGCT GAAGGCGTCC TCTATGAAGA CTCTGAACTC GAAGACGCTC 
TACGTGAAGG TTCTTCCAAC AACAGCAGAA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956190.2
CDS	53..3868
Translation	MVEAVPESIHFPTEEEQILQFWQKNDCFQECLKQSKSRPRFTFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQNGFHVERRFGWDCHGLPVEYEIDKSLNIKGPEDVMKMGIEEYNKQCRSIVMRYADEWENSVRRMGRWIDFRNDYKTLYPWFMETVWWVFKQLYDSGLVYRGVKVMPFSTACNTPLSNFEANQNYKDVQDPSVIVNFPLLEDPSVSLIAWTTTPWTLPSNLALCVNPELIYVRVKDNSSQKIYIMMEARLVALFKSDSEYTILERFPGKTLKGKKYKPLFEYFIKCKETGAFSVVMDNYVRDDDGTGVVHQAPYFGADDYRVCMEYDIIQRDSAPICPVDPSGIFTPEVTHFAGQYVKDADKNIIKWLKENGRLVSASSFKHSYPFCWRSDTPLIYKAVPSWFIRVEHMVEKLLDNNSKCYWVPEFVKEKRFGNWLRDARDWAVSRNRYWGTPIPLWVSDDFEEVVCVGSMAELEELTGIKVTDLHRESIDGLTIPSRCGKGQLRRVSEVFDCWFESGSMPYAQVHYPFENRREFEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGKPPFKNVIVNGLVLASDGQKMSKRKKNYPDPGLIVQSYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVFLPWYNAYRFLVQNVQRLQKEEGVEFLYNERTSSVSNNIMDKWIQSFTQSLIQFFRDEMGAYRLYTVVPKLVKFVDMLTNWYVRMNRRRLKGESGSEDCVRALETLFSVLFSMCRLMAPFTPFITELMYQNLRHLLDPAAVEEKDTGSIHYLMLPQVRESLIDQRIESAVSRMQSVIELGRVIRDRKTLPIKYPLKEVVVIHQDPEALTDIQSLQNYILEELNVRQLTLSTDKDRYGIRLRAEPDHMVLGKRLKGAFKSVTASIKELKSEELEEFQKTGCIVVDGHDLREEDLRLMYTFDQSSGSASQYEAHSDAQVLVLLDVTPDQAMLDEGVAREVINRIQKLRKKGHLVPSDEITVFYRCQPAGEYLHQVIEAHTDFILATTKAPLRAYPVCPNASVIVQENTQLKGSDLELTIVRGSSSSSVCRAPVSGSACAFVNLRVSLKDKQQDGVVLLENPKGDNRLDLEQLKRICCSVFGLNGAPVSIFNGKTALSSSTDLLSLSGRTLTVSSAAGSSPADVSSSESLLCPYVNLQLVNASPAECRTGDVATLLLMNPEGKNLLQFSDLLRETAKVFGLRGRRLMLYTDPQLTAELKASSMKTLNSKTLYVKVLPTTAEA

Target ORF information:

RefSeq Version	NM_199896.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio isoleucyl-tRNA synthetase 1 (iars1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199896.2

ORF Insert Sequence:

ATGGTGGAAG CAGTGCCAGA GTCCATCCAC TTCCCCACTG AGGAGGAGCA AATCCTGCAG 
TTCTGGCAGA AGAACGACTG TTTCCAGGAG TGTCTGAAGC AGTCAAAGAG CCGGCCCAGG 
TTCACGTTCT ACGACGGGCC GCCCTTCGCC ACAGGTCTGC CTCACTATGG TCATATTCTG 
GCGGGAACCA TCAAGGACAT CGTGACGCGA TTCGCTCATC AGAACGGCTT CCACGTGGAG 
CGGCGCTTCG GCTGGGACTG CCATGGCCTG CCGGTGGAGT ATGAGATCGA TAAATCTCTG 
AACATCAAAG GACCCGAGGA TGTGATGAAG ATGGGCATCG AGGAGTACAA CAAACAGTGC 
CGGAGCATCG TCATGAGATA TGCTGATGAG TGGGAGAACT CGGTGAGGCG GATGGGGCGC 
TGGATTGACT TCAGGAACGA CTATAAGACC CTCTACCCCT GGTTCATGGA GACTGTGTGG 
TGGGTGTTTA AGCAGCTGTA TGACAGTGGT CTGGTGTACC GCGGGGTGAA GGTGATGCCG 
TTCTCCACCG CCTGCAACAC TCCGCTCTCC AACTTTGAGG CCAATCAGAA CTACAAGGAT 
GTGCAGGACC CGTCAGTGAT CGTCAATTTC CCGTTGCTGG AGGATCCGTC TGTCAGCTTG 
ATCGCCTGGA CCACCACACC CTGGACCCTC CCCAGCAACC TGGCACTCTG CGTCAACCCA 
GAGCTCATCT ACGTCCGGGT CAAAGACAAC AGCTCACAGA AGATCTACAT CATGATGGAG 
GCCAGACTCG TCGCTCTCTT CAAGTCTGAC TCTGAATACA CCATTCTGGA GCGATTTCCT 
GGCAAGACTC TGAAGGGCAA AAAATACAAG CCTCTTTTCG AGTACTTCAT CAAGTGTAAG 
GAGACCGGTG CCTTCTCGGT AGTGATGGAT AATTATGTTC GTGATGATGA CGGTACAGGA 
GTCGTCCATC AGGCCCCGTA CTTCGGAGCT GATGATTATC GTGTGTGTAT GGAGTATGAC 
ATCATCCAGA GAGACTCCGC CCCCATCTGC CCGGTCGACC CATCTGGCAT ATTCACTCCG 
GAGGTCACAC ACTTCGCCGG ACAGTATGTG AAGGATGCAG ACAAAAACAT CATCAAGTGG 
CTGAAGGAGA ACGGACGTCT GGTGAGCGCC AGCTCCTTCA AGCACAGCTA CCCGTTCTGC 
TGGAGGTCGG ACACGCCGCT GATCTACAAA GCAGTGCCCA GCTGGTTTAT AAGGGTGGAG 
CACATGGTGG AGAAACTTCT GGACAACAAC AGCAAGTGCT ACTGGGTGCC AGAGTTTGTG 
AAGGAGAAGC GTTTCGGGAA CTGGCTGAGG GACGCCAGGG ACTGGGCGGT GTCCCGCAAC 
CGCTACTGGG GCACACCTAT CCCTCTGTGG GTCAGTGACG ACTTCGAGGA GGTGGTGTGT 
GTGGGATCCA TGGCGGAGCT GGAGGAGCTG ACGGGAATTA AAGTCACTGA TCTCCACCGA 
GAAAGCATTG ACGGCCTCAC CATCCCCTCT CGCTGTGGGA AAGGGCAGCT CCGGCGGGTT 
TCGGAGGTGT TCGACTGCTG GTTCGAGAGC GGCAGTATGC CCTACGCTCA GGTGCACTAC 
CCGTTTGAGA ACAGGCGCGA GTTCGAAGAC GCATTCCCTG CAGACTTCAT CGCAGAGGGC 
ATCGACCAGA CCAGAGGATG GTTCTACACG TTACTCGTGC TCTCGACGGC TCTGTTTGGG 
AAACCGCCAT TCAAGAACGT GATCGTCAAC GGTTTGGTTT TGGCCAGTGA TGGGCAGAAA 
ATGAGCAAGC GGAAGAAGAA TTACCCCGAC CCCGGACTCA TCGTGCAGAG CTACGGTGCA 
GACGCACTAC GGCTGTATCT GATCAACTCT CCTGTGGTTC GAGCTGAAAA CCTGCGCTTC 
AAAGAGGAAG GAGTTCGAGA TGTGCTGAAA GACGTCTTCC TACCCTGGTA CAATGCATAC 
CGGTTCCTGG TGCAGAACGT CCAGAGACTG CAGAAGGAGG AAGGCGTTGA GTTCCTGTAC 
AACGAGAGGA CGTCCAGCGT CAGCAACAAC ATCATGGATA AATGGATCCA GTCTTTCACA 
CAGTCACTCA TTCAGTTCTT CCGGGACGAG ATGGGAGCCT ACAGACTCTA TACTGTGGTG 
CCCAAGCTTG TCAAGTTTGT GGATATGCTC ACTAACTGGT ACGTGCGGAT GAACCGCCGG 
CGGCTGAAGG GTGAAAGTGG TTCTGAGGAC TGCGTTCGTG CTCTGGAAAC CCTCTTCAGT 
GTCCTGTTCT CCATGTGCAG ACTGATGGCT CCGTTCACTC CGTTTATAAC CGAGCTGATG 
TACCAGAACC TGCGGCACCT GCTGGACCCG GCAGCAGTGG AGGAGAAGGA CACCGGCAGC 
ATACACTACC TCATGCTGCC ACAAGTCAGG GAGTCTCTGA TTGACCAGCG TATTGAGAGT 
GCGGTGTCTC GGATGCAGTC GGTCATTGAG TTGGGTCGTG TAATCAGAGA CAGGAAGACT 
CTGCCCATCA AGTATCCGCT GAAGGAGGTG GTGGTCATCC ATCAGGACCC AGAGGCTCTC 
ACTGATATCC AGTCCCTGCA GAACTACATC CTGGAGGAGC TGAACGTGCG CCAGCTCACT 
CTCTCCACTG ATAAGGACCG ATACGGCATC CGTCTGCGGG CCGAACCCGA TCACATGGTG 
CTGGGCAAAC GGCTGAAAGG AGCCTTTAAA TCCGTGACCG CGTCCATCAA AGAGCTGAAG 
AGTGAAGAGC TGGAGGAGTT CCAGAAGACA GGTTGTATAG TGGTGGATGG TCATGACCTG 
CGTGAGGAGG ATCTGCGTCT TATGTACACC TTCGATCAGT CGTCTGGATC CGCGTCTCAG 
TACGAGGCTC ACTCAGACGC TCAGGTGCTG GTTCTGCTGG ACGTCACGCC GGATCAGGCC 
ATGCTGGACG AAGGAGTGGC CCGAGAGGTC ATCAACCGCA TCCAGAAACT GCGTAAGAAG 
GGTCACCTGG TGCCGTCAGA TGAGATCACG GTGTTCTACC GCTGTCAGCC GGCTGGTGAA 
TATCTGCATC AGGTGATTGA GGCACACACA GACTTCATCC TGGCCACCAC TAAAGCTCCG 
CTCAGAGCGT ACCCCGTGTG TCCCAACGCC AGCGTCATCG TGCAGGAGAA CACACAGCTG 
AAGGGCTCAG ATCTGGAGTT GACTATCGTG CGAGGCTCCT CCTCTTCCTC CGTCTGCAGG 
GCTCCTGTGA GCGGCTCGGC GTGTGCGTTT GTGAACCTCA GAGTGTCGCT CAAAGATAAA 
CAGCAGGACG GTGTGGTTCT GCTGGAGAAC CCCAAGGGAG ACAACAGACT GGACCTGGAG 
CAGCTGAAGA GGATCTGCTG CAGTGTGTTC GGCCTCAACG GAGCTCCAGT CTCCATATTC 
AACGGCAAGA CAGCACTCAG CAGCAGCACT GATCTGCTGT CTCTGAGCGG CAGGACTCTG 
ACTGTGAGCT CGGCTGCGGG TTCGAGTCCT GCAGATGTGA GCAGCTCCGA GTCTCTGCTG 
TGTCCGTATG TGAACCTGCA GCTCGTCAAC GCCAGTCCTG CAGAGTGTCG GACTGGAGAC 
GTGGCGACGC TGCTCCTGAT GAACCCTGAG GGTAAAAACC TCCTCCAGTT CTCAGATCTG 
CTGAGAGAAA CGGCTAAAGT GTTCGGTCTG AGAGGAAGAA GACTGATGCT CTACACTGAT 
CCACAGCTCA CTGCAGAGCT GAAGGCGTCC TCTATGAAGA CTCTGAACTC GAAGACGCTC 
TACGTGAAGG TTCTTCCAAC AACAGCAGAA GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

A single Danio rerio hars gene encodes both cytoplasmic and mitochondrial histidyl-tRNA synthetases.
PloS one12(9)e0185317(2017)
Waldron AL,Cahan SH,Franklyn CS,Ebert AM

Biallelic IARS Mutations Cause Growth Retardation with Prenatal Onset, Intellectual Disability, Muscular Hypotonia, and Infantile Hepatopathy.
American journal of human genetics99(2)414-22(2016 Aug)
Kopajtich R,Murayama K,Janecke AR,Haack TB,Breuer M,Knisely AS,Harting I,Ohashi T,Okazaki Y,Watanabe D,Tokuzawa Y,Kotzaeridou U,K?lker S,Sauer S,Carl M,Straub S,Entenmann A,Gizewski E,Feichtinger RG,Mayr JA,Lackner K,Strom TM,Meitinger T,M?ller T,Ohtake A,Hoffmann GF,Prokisch H,Staufner C

Aminoacyl-Transfer RNA Synthetase Deficiency Promotes Angiogenesis via the Unfolded Protein Response Pathway.
Arteriosclerosis, thrombosis, and vascular biology36(4)655-62(2016 Apr)
Castranova D,Davis AE,Lo BD,Miller MF,Paukstelis PJ,Swift MR,Pham VN,Torres-V?zquez J,Bell K,Shaw KM,Kamei M,Weinstein BM

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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iars1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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