MGG_03220 cDNA ORF clone, Pyricularia oryzae 70-15

Gene
Clones
gRNAs

Summary

Gene Symbol	MGG_03220
Entrez Gene ID	2676419
Full Name	isoleucyl-tRNA synthetase
Gene Type	protein-coding
Organism	Pyricularia oryzae 70-15

mRNA and Protein(s)

mRNA	Protein	Name
XM_003716711.1	XP_003716759.1	isoleucyl-tRNA synthetase

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the rice blast fungus Magnaporthe grisea.
Dean RA, Talbot NJ, Ebbole DJ, Farman ML, Mitchell TK, Orbach MJ, Thon M, Kulkarni R, Xu JR, Pan H, Read ND, Lee YH, Carbone I, Brown D, Oh YY, Donofrio N, Jeong JS, Soanes DM, Djonovic S, Kolomiets E, Rehmeyer C, Li W, Harding M, Kim S, Lebrun MH, Bohnert H, Coughlan S, Butler J, Calvo S, Ma LJ, Nicol R, Purcell S, Nusbaum C, Galagan JE, Birren BW
Nature434(7036)980-6(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MGG_03220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_03220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe01829	XM_003716711.1 Latest version!	Pyricularia oryzae 70-15 isoleucyl-tRNA synthetase (MGG_03220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MGG_03220 ( XM_003716711.1 ) cDNA ORF clone, Pyricularia oryzae 70-15 -> XP_003716759.1 Pyricularia oryzae 70-15 isoleucyl-tRNA synthetase (MGG_03220), mRNA.

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CloneID	OMe01829
Clone ID Related Accession (Same CDS sequence)	XM_003716711.1
Accession Version	XM_003716711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-28
Organism	Pyricularia oryzae 70-15
Product	isoleucyl-tRNA synthetase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017851).

ATGTCTATCA GCTTCCCGAA GGAGGAGGAG GCGGTCATCG CCAGATGGCG TGAGATCAAT 
GCTTTCGAGA GACAGGTCGA GCTCTCTCAA GGCCGTCCTC TGTATACCTT CTACGATGGT 
CCCCCTTTCG CCACTGGACT TCCTCACTAT GGCCACCTGC TCGCTTCTAC CATCAAGGAC 
ATCATCCCCA GGTATTGGTC CATGAAGGGT TACCATGTTG AGAGGCGGTT CGGGTGGGAT 
ACTCACGGCC TGCCTATTGA ACATGAGATC GACAAGAAGC ATGGAATCTC CGGCAAAGCA 
GCAGTTGAAA AGTTTGGCCT TGCCAACTAC AATGAGGAGT GCCGGTCTAT TGTTATGCGC 
TACCGCGAAG AATGGAGGCA TACTATCGAG CGGTTGGGTC GGTGGATTGA TTTCGACAAC 
GATTACAAGA CCATGGACCC CAAATTCATG GAATCGGAGT GGTGGGTTTT CAAACAGCTG 
TTCGAAAAGG GACAAGTCTA CCAGGGCTTC CGTGTGATGC CATACTCGAC TGTCCTTACC 
ACAGCTCTGA GCAACTTCGA GGCAAACCAA AATTACCAGG ACGTCACAGA CCCTGCCGTT 
GTGGTCTCTT TCCCGCTTAA GTCAGACCCA GAGACTTCCC TGCTTGCTTG GACGACAACA 
CCGTGGACAT TACCCTCGAA CCTTGGCCTT GCAGCACACC CTGATTTCGA ATACGTCAAG 
ATTCGCGACG AAAAGTCCGG CAAGAACTAC ATTCTTCTAG AAAAATTGAT GGGAACTTTG 
TACAAGGATC CCAAGAAAGC CAAGTTCCAA ATCCTTCAGA AAATCAAAGG CAAGGATATG 
CTTGGTTGGG AGTATGAACC TTTGTTCGAC TACTTCTACG AGGAGTTCAA GGATGTCGGA 
TTCCGCGTCC TGAACGCCAC CTATGTTACT GACGACGGTG GTGTCGGTAT CGTTCACCAG 
GCTCCCGCTT TCGGTGAAGA TGATTACAAT GTTGCATCTG AAGCAGGAAT CATCAACGAG 
AACCGGCCAC CAGCAGACCC TGTCAACGAG ACCGGCCACT TCACCGACAA GGTTCGCGAC 
TTTGCAGGCA TGCACGTCAA AGAAGCCGAT AAACACATCA TCAAATATCT GAAAGGCACG 
GGGCGCTTGG TAGCCGACTC ACAACTCAAG CACTCTTACC CTATGTGCCC AAGATCTGAT 
ACCCCTCTTA TTTACCGCGC CGTTCCATCA TGGTTTGTGC GCATTCCAGA GATCGTACCG 
AAGATGCTCG AGAATATCGA GACGCAACAT TGGGTCCCAA GTTTCGTCAA AGAGAAACGC 
TTTGCCAGCT GGATCGCGAA CGCGAGGGAC TGGAATGTGT CGCGCAACAG GTATTGGGGT 
ACACCTATCC CGCTCTGGGT CAGTGATGAT CTTGAGGAGA GGGTATGTGT TGGCAGCATT 
GAGGAGCTCA AGAAGCTCAG TGGTTACGAG GGAGAAATCA CCGATCTACA CCGACACCAC 
ATCGATCACA TCACCATCCC CAGCAAGCAG GGCAAGGGCG TTCTGAAGCG TATTGAGGAA 
GTCTTTGATT GTTGGTTCGA GTCTGGTTCC ATGCCTTACG CCAGCCAACA CTACCCCTTT 
GAAAATGTCG AGAAGTTTGA GAAATCATTC CCAGGCGATT TCATTGCCGA GGGTCTGGAT 
CAGACGCGTG GTTGGTTTTA CACAATGCTG GTTCTCGGCA CCCATCTCTT TGGTATTGCA 
CCATTCAAGA ACTGTGTTGT AAACGGCATT GTGCTGGCCG AGGACGGAAA GAAGATGTCG 
AAGAGGCTCA AGAACTACCC GGCGCCTGAC ATCGTCATGG ACAAATATGG ATCTGATGCG 
CTGCGATTAT ATCTGATCAA CTCCCCGGTG GTGCGGGCTG AGCCACTTCG TTTCAAGGAG 
ACCGGCGTGA AGGAGGTCAT TCAAAGGGTC CTGCTACCAT TCTGGAACAG CTATAAATTC 
TTCGAGGGAC AAGTCGCTCT TTTGAAGAAG GCCGAAGAAA AGGACTATAT GTGGAACCCC 
ACTGCAGAAT CCTCAAACGA GAACGTGATG GACCGCTGGA TCTTGGCCAG CTGTCAAAGT 
CTTCTTGAGT TCGTGAACGA AGAGATGAGG CATTACCGAC TTTACACTGT CGTCCCCCGC 
TTACTGGGCC TTATTGACAA CACGACGAAC TGGTACATTC GTTTCAACCG AAAGCGTCTC 
AAGGGCGAGA ATGGCGTAGA AGATACCGTA CACGCGCTCA ACACCCTCTT CGAGGTGTTG 
TTTACCCTTT GCAAGGGACT CGCTCCGTTC ACGCCTTTCA TCACGGAAAC CATCTACCAG 
AAGCTGGCGC CGTTCATCCC TAAAGACCTG CCCAAGGAGC TGCAGAGCAG CGACATGAGG 
AGTGTTCACT TTTTGCCGTT CCCGACTGTT CGACAGGAGC TGTTTGACCC AGTGGTTGAA 
CGCAAAGTCG GGCGTATGCA GAAGGTCATC GAGCTTGCCC GAGTGTCTCG TGAGAGGAGA 
GCCATCGGCT TGAAAACGCC ACTCAAGACA CTGATCGTTA TCCACCATAG CCAAGAGTAC 
TTGGACGATG TAAAATCTCT CCAGGGCTAT ATTAGCGAGG AGCTCAACAT ACGGGATCTT 
GTTTTGACGT CAGACGAGGC CAAGTATGGT GTTCAGTACA GCGCTACGGC TGACTGGCCA 
GTTCTTGGAA AGAAGCTCAA GAAGGACATG GCCAAGGTCA AGAAAGCGCT GCCCCTGCTC 
CAAAGCGAGC AGGTTCACGC ATATGTGCAG CAGGGGACGA TTGAGGTTGA CGGCATTAAG 
CTGGATGCTG GAGATTTGGT GGTCAAGCGC GGATTGAGCC CGGCGGCGGA TGGATCCTCG 
CAGTACCTCG AGACAAACAC AGATAACGAT GTGCTCATCA TCCTTGACTG CGAACTCCAT 
GCTGATCTTG CAGATGAGGG TTTGGCACGA GAGATTATCA ATCGTGTGCA GAGGCTGCGT 
AAAAAGGCGG GCTTGGTAGC TACTGATGAT GTGCGGATGG AGTACAAGGT GCTGGGAGAC 
CCCGAGACCA CCGGCATCGA AAAGGTTTTC CAGTCGCAGT CGAGCATGCT AGAGAAAGCC 
TTGAGAAGAC CCATGGAGGG CGAGGTCGTC GACCAGCCCG AGGACGGTGT CATTGCAGAG 
GAGATGCAAG AAGTCCAGAA GGCTACGTTC AGGCTCCGGT TGCTGAAGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003716759.1
CDS	278..3511
Translation	MSISFPKEEEAVIARWREINAFERQVELSQGRPLYTFYDGPPFATGLPHYGHLLASTIKDIIPRYWSMKGYHVERRFGWDTHGLPIEHEIDKKHGISGKAAVEKFGLANYNEECRSIVMRYREEWRHTIERLGRWIDFDNDYKTMDPKFMESEWWVFKQLFEKGQVYQGFRVMPYSTVLTTALSNFEANQNYQDVTDPAVVVSFPLKSDPETSLLAWTTTPWTLPSNLGLAAHPDFEYVKIRDEKSGKNYILLEKLMGTLYKDPKKAKFQILQKIKGKDMLGWEYEPLFDYFYEEFKDVGFRVLNATYVTDDGGVGIVHQAPAFGEDDYNVASEAGIINENRPPADPVNETGHFTDKVRDFAGMHVKEADKHIIKYLKGTGRLVADSQLKHSYPMCPRSDTPLIYRAVPSWFVRIPEIVPKMLENIETQHWVPSFVKEKRFASWIANARDWNVSRNRYWGTPIPLWVSDDLEERVCVGSIEELKKLSGYEGEITDLHRHHIDHITIPSKQGKGVLKRIEEVFDCWFESGSMPYASQHYPFENVEKFEKSFPGDFIAEGLDQTRGWFYTMLVLGTHLFGIAPFKNCVVNGIVLAEDGKKMSKRLKNYPAPDIVMDKYGSDALRLYLINSPVVRAEPLRFKETGVKEVIQRVLLPFWNSYKFFEGQVALLKKAEEKDYMWNPTAESSNENVMDRWILASCQSLLEFVNEEMRHYRLYTVVPRLLGLIDNTTNWYIRFNRKRLKGENGVEDTVHALNTLFEVLFTLCKGLAPFTPFITETIYQKLAPFIPKDLPKELQSSDMRSVHFLPFPTVRQELFDPVVERKVGRMQKVIELARVSRERRAIGLKTPLKTLIVIHHSQEYLDDVKSLQGYISEELNIRDLVLTSDEAKYGVQYSATADWPVLGKKLKKDMAKVKKALPLLQSEQVHAYVQQGTIEVDGIKLDAGDLVVKRGLSPAADGSSQYLETNTDNDVLIILDCELHADLADEGLAREIINRVQRLRKKAGLVATDDVRMEYKVLGDPETTGIEKVFQSQSSMLEKALRRPMEGEVVDQPEDGVIAEEMQEVQKATFRLRLLKL

Target ORF information:

RefSeq Version	XM_003716711.1
Organism	Pyricularia oryzae 70-15
Definition	Pyricularia oryzae 70-15 isoleucyl-tRNA synthetase (MGG_03220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003716711.1

ORF Insert Sequence:

ATGTCTATCA GCTTCCCGAA GGAGGAGGAG GCGGTCATCG CCAGATGGCG TGAGATCAAT 
GCTTTCGAGA GACAGGTCGA GCTCTCTCAA GGCCGTCCTC TGTATACCTT CTACGATGGT 
CCCCCTTTCG CCACTGGACT TCCTCACTAT GGCCACCTGC TCGCTTCTAC CATCAAGGAC 
ATCATCCCCA GGTATTGGTC CATGAAGGGT TACCATGTTG AGAGGCGGTT CGGGTGGGAT 
ACTCACGGCC TGCCTATTGA ACATGAGATC GACAAGAAGC ATGGAATCTC CGGCAAAGCA 
GCAGTTGAAA AGTTTGGCCT TGCCAACTAC AATGAGGAGT GCCGGTCTAT TGTTATGCGC 
TACCGCGAAG AATGGAGGCA TACTATCGAG CGGTTGGGTC GGTGGATTGA TTTCGACAAC 
GATTACAAGA CCATGGACCC CAAATTCATG GAATCGGAGT GGTGGGTTTT CAAACAGCTG 
TTCGAAAAGG GACAAGTCTA CCAGGGCTTC CGTGTGATGC CATACTCGAC TGTCCTTACC 
ACAGCTCTGA GCAACTTCGA GGCAAACCAA AATTACCAGG ACGTCACAGA CCCTGCCGTT 
GTGGTCTCTT TCCCGCTTAA GTCAGACCCA GAGACTTCCC TGCTTGCTTG GACGACAACA 
CCGTGGACAT TACCCTCGAA CCTTGGCCTT GCAGCACACC CTGATTTCGA ATACGTCAAG 
ATTCGCGACG AAAAGTCCGG CAAGAACTAC ATTCTTCTAG AAAAATTGAT GGGAACTTTG 
TACAAGGATC CCAAGAAAGC CAAGTTCCAA ATCCTTCAGA AAATCAAAGG CAAGGATATG 
CTTGGTTGGG AGTATGAACC TTTGTTCGAC TACTTCTACG AGGAGTTCAA GGATGTCGGA 
TTCCGCGTCC TGAACGCCAC CTATGTTACT GACGACGGTG GTGTCGGTAT CGTTCACCAG 
GCTCCCGCTT TCGGTGAAGA TGATTACAAT GTTGCATCTG AAGCAGGAAT CATCAACGAG 
AACCGGCCAC CAGCAGACCC TGTCAACGAG ACCGGCCACT TCACCGACAA GGTTCGCGAC 
TTTGCAGGCA TGCACGTCAA AGAAGCCGAT AAACACATCA TCAAATATCT GAAAGGCACG 
GGGCGCTTGG TAGCCGACTC ACAACTCAAG CACTCTTACC CTATGTGCCC AAGATCTGAT 
ACCCCTCTTA TTTACCGCGC CGTTCCATCA TGGTTTGTGC GCATTCCAGA GATCGTACCG 
AAGATGCTCG AGAATATCGA GACGCAACAT TGGGTCCCAA GTTTCGTCAA AGAGAAACGC 
TTTGCCAGCT GGATCGCGAA CGCGAGGGAC TGGAATGTGT CGCGCAACAG GTATTGGGGT 
ACACCTATCC CGCTCTGGGT CAGTGATGAT CTTGAGGAGA GGGTATGTGT TGGCAGCATT 
GAGGAGCTCA AGAAGCTCAG TGGTTACGAG GGAGAAATCA CCGATCTACA CCGACACCAC 
ATCGATCACA TCACCATCCC CAGCAAGCAG GGCAAGGGCG TTCTGAAGCG TATTGAGGAA 
GTCTTTGATT GTTGGTTCGA GTCTGGTTCC ATGCCTTACG CCAGCCAACA CTACCCCTTT 
GAAAATGTCG AGAAGTTTGA GAAATCATTC CCAGGCGATT TCATTGCCGA GGGTCTGGAT 
CAGACGCGTG GTTGGTTTTA CACAATGCTG GTTCTCGGCA CCCATCTCTT TGGTATTGCA 
CCATTCAAGA ACTGTGTTGT AAACGGCATT GTGCTGGCCG AGGACGGAAA GAAGATGTCG 
AAGAGGCTCA AGAACTACCC GGCGCCTGAC ATCGTCATGG ACAAATATGG ATCTGATGCG 
CTGCGATTAT ATCTGATCAA CTCCCCGGTG GTGCGGGCTG AGCCACTTCG TTTCAAGGAG 
ACCGGCGTGA AGGAGGTCAT TCAAAGGGTC CTGCTACCAT TCTGGAACAG CTATAAATTC 
TTCGAGGGAC AAGTCGCTCT TTTGAAGAAG GCCGAAGAAA AGGACTATAT GTGGAACCCC 
ACTGCAGAAT CCTCAAACGA GAACGTGATG GACCGCTGGA TCTTGGCCAG CTGTCAAAGT 
CTTCTTGAGT TCGTGAACGA AGAGATGAGG CATTACCGAC TTTACACTGT CGTCCCCCGC 
TTACTGGGCC TTATTGACAA CACGACGAAC TGGTACATTC GTTTCAACCG AAAGCGTCTC 
AAGGGCGAGA ATGGCGTAGA AGATACCGTA CACGCGCTCA ACACCCTCTT CGAGGTGTTG 
TTTACCCTTT GCAAGGGACT CGCTCCGTTC ACGCCTTTCA TCACGGAAAC CATCTACCAG 
AAGCTGGCGC CGTTCATCCC TAAAGACCTG CCCAAGGAGC TGCAGAGCAG CGACATGAGG 
AGTGTTCACT TTTTGCCGTT CCCGACTGTT CGACAGGAGC TGTTTGACCC AGTGGTTGAA 
CGCAAAGTCG GGCGTATGCA GAAGGTCATC GAGCTTGCCC GAGTGTCTCG TGAGAGGAGA 
GCCATCGGCT TGAAAACGCC ACTCAAGACA CTGATCGTTA TCCACCATAG CCAAGAGTAC 
TTGGACGATG TAAAATCTCT CCAGGGCTAT ATTAGCGAGG AGCTCAACAT ACGGGATCTT 
GTTTTGACGT CAGACGAGGC CAAGTATGGT GTTCAGTACA GCGCTACGGC TGACTGGCCA 
GTTCTTGGAA AGAAGCTCAA GAAGGACATG GCCAAGGTCA AGAAAGCGCT GCCCCTGCTC 
CAAAGCGAGC AGGTTCACGC ATATGTGCAG CAGGGGACGA TTGAGGTTGA CGGCATTAAG 
CTGGATGCTG GAGATTTGGT GGTCAAGCGC GGATTGAGCC CGGCGGCGGA TGGATCCTCG 
CAGTACCTCG AGACAAACAC AGATAACGAT GTGCTCATCA TCCTTGACTG CGAACTCCAT 
GCTGATCTTG CAGATGAGGG TTTGGCACGA GAGATTATCA ATCGTGTGCA GAGGCTGCGT 
AAAAAGGCGG GCTTGGTAGC TACTGATGAT GTGCGGATGG AGTACAAGGT GCTGGGAGAC 
CCCGAGACCA CCGGCATCGA AAAGGTTTTC CAGTCGCAGT CGAGCATGCT AGAGAAAGCC 
TTGAGAAGAC CCATGGAGGG CGAGGTCGTC GACCAGCCCG AGGACGGTGT CATTGCAGAG 
GAGATGCAAG AAGTCCAGAA GGCTACGTTC AGGCTCCGGT TGCTGAAGCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the rice blast fungus Magnaporthe grisea.
Nature434(7036)980-6(2005 Apr)
Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW

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MGG_03220 cDNA ORF clone, Pyricularia oryzae 70-15

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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