ORF » Species Summary » Schizosaccharomyces pombe » irs1 cDNA ORF clone

irs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following irs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100438 NM_001021464.2
Latest version!		 Schizosaccharomyces pombe putative isoleucine--tRNA(Ile) ligase Irs1 (irs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    irs1 ( NM_001021464.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595569.1 Schizosaccharomyces pombe putative isoleucine--tRNA(Ile) ligase Irs1 (irs1), mRNA.
    Price & Availability↑
    CloneID OSc100438
    Clone ID Related Accession (Same CDS sequence) NM_001021464.2
    Accession Version NM_001021464.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative isoleucine--tRNA(Ile) ligase Irs1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021464.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCGTTTA ACGTTCCTAA AGAAGAGGAA AAAATCGTTG AGTTTTGGCG CGAAATCGAT 
    GCGTTCCATA CTCAGTTAAA ACTTTCTCAA GGTCGTCCTA CTTATACATT TTTCGATGGA 
    CCTCCATTCG CTACCGGTCG TCCTCATCAT GGTCATCTTT TGGCTTCTAC CATTAAGGAT 
    AGTGTCACCC GTTATGCTTG CTTGAAGGGT TATCACGTCG AGCGTAGATT CGGTTGGGAT 
    ACACATGGAC TTCCTGTTGA GCATGAAATC GACAAAAAGT TGGGTATTAC TGGAAGTGAT 
    GATGTTATGG CTATGGGTAT TGACAAATAC AATGCCGAAT GTCGTAAAAT TGTCATGACT 
    TATGCATCCG AATGGCGTGC AACAGTTGAA AGACTGGGCC GTTGGATTGA TTTTGATAAC 
    GATTATAAAA CGTTGTATCC TTCTTTCATG GAAAGTGTTT GGTGGGTTTT CAAAGAGTTG 
    CATACAAAAG GAAAAGTTTA CCGCGGATAT CGTGTTATGC CTTACTCGAC TGCATGTACA 
    ACACCTTTGA GTAACTTTGA AGCTCAACAA AACTATAAGG AAGTCCCAGA TCCGGCTATT 
    GTTGTTGCCT TTCAATCCAT TAGTGATCCT GAAGTTTCTT TCTTAGCTTG GACTACTACT 
    CCATGGACCT TGCCTTCTAA CCTTGCTTTG GCTGTTCACC CTGACTTACA GTACATAAAA 
    ATTTTGGACA AGGATTCTAA TAAGAAATAT ATCCTTATGG AGAGCTGCCT TGGCATCCTT 
    TACAAGAACC CAAAAAAGGC GAACTTTGAA ATTTTGGAAC GTTTCCAAGG AAAGGCCTTG 
    GATGGTCAAA AGTATGAACC TCTCTTCCCT TATTTCAAGA GCACTTTTGG CGAAAGAGCA 
    TTTAAATTAT ATTCTGCTGA CTACGTTGAA GAAGGCAGTG GTACTGGTAT CGTCCATCAA 
    GCTCCTGCTT TTGGTGAAGC AGATTATGAC GCTGCTTGGG CTGCTGGCAT CATCGATGCT 
    GATCACCAAC CTCCTTGCCC TGTTGATGAG CAAGGTTTGC TTACTTCGGA AATCACAGAC 
    TTCGCTGGCC AATATGTTAA AGACGCAGAT AAGGAAATTA TCCGCTCCTT GAAAGCATCT 
    GGCCATTTGG TTAAGCATAG TCAAATTTTC CATAGTTATC CATTTTGCTG GCGTTCCGAT 
    ACACCTCTAA TTTATCGTGC CGTTCCCAGT TGGTTTGTTC GCGTGAAGGA GATCACTAAT 
    GAGATGGTTG AAAATGTCAT GAGCACTCAC TGGGTCCCTC AAAACATTCG TGATAAACGT 
    TTTGCGAATT GGTTGAAGAA TGCTCGTGAC TGGAACATTT CCCGTAACAG ATACTGGGGT 
    ACCCCTATCC CACTTTGGGT TAGTGATGAC TACGAAGAAG TCGTTTGCAT TGGTTCCATT 
    AAGGAGCTTG AAGAACTTTC CGGCGTTTCT AATATTACAG ACATTCATCG TGACAGTATT 
    GATCATATCA CTATTCCTTC TAAAAAAGGT AAAGGAACAT TGCATCGTGT CAGTGAAGTA 
    TTTGACTGTT GGTTTGAATC TGGCAGCATG CCTTATGCTT CTCGTCATTA CCCTTTCGAA 
    AGAATTGAGG AATTCAAGCA TGGCTTCCCT GCTGACTTTA TTTCCGAGGG TGTTGATCAA 
    ACCCGTGGAT GGTTTTATAC TTTAACTGTT TTGGGAACTT TGTTATTCGA CAAAGCACCT 
    TATAAAAATG TAATTGTTAG TGGTCTGGTA ATGGCTGAGG ATGGTAAAAA AATGTCCAAA 
    CGTCTTAAAA ATTACCCTGA ACCAAATCTC ATCATTGAAA AATATGGTTC TGATGCCCTT 
    CGTTTGTATC TCATCAACTC TCCAGTTGTT CGTGCTGAGA TTCTTAAATT TAAGGAAGAT 
    GGTGTAAGAG AAGTTGTTAC TAGAGTTTTG ATTCCTTGGT GGAACAGTTA TAAGTTTTTT 
    GAGGCGCAAG CTGCTCTTTA CAAGAAGGTT ACTGGTAAAG ACTTTGTTTT CGATGATGCT 
    GCCACTTTGA GTTCCAATGT AATGGATCGC TGGATTTTAG CTCGTTGCCA GAGCTTGATT 
    GGATTTGTTG ATGAAGAAAT GAAACAATAC CGTTTGTACA CCGTGGTCCC TCAACTTCTC 
    GGTTTAATTG AGGAGATGAC TAACTGGTAT ATCCGGTTTA ACAGACGTCG TTTGAAGGGT 
    GAAGATGGTG AAATTGAAAC TATTAATGCT TTGAACGTTT TGTTTGAAGT CTTGTTCACG 
    TTAGTTCGTA TTATGGGCCC ATTCACCCCT TTCATTACTG AAAACATTTA TCAACACCTT 
    AGAAACTACA TGCCTATTGA CAAAAATGAA ATTTCACTTC GTTCAGTTCA TTTCCTTCCT 
    TTCCCTACTT ACAAGTCTGA GCTTGATGAT GAAACTGTTT TACGCCGTGT TAAGCGTATG 
    CAAACTATTA TCGAATTGGC GCGTTATGTT CGTGAGCAGA ATAATATTTC TTTGAAGACT 
    CCCTTAAAAA CCCTTATTGT TATTCTCACT AACGAAGAAT ACTTGGAAGA TGCTAAGCTC 
    TTAGAAAGAT ATATTGCAGA GGAGCTTAAC GTCCGTGAAG TAGTTTTCAC CTCCAATGAA 
    GAGAAGTATG GAGTCGTATA CAGCGTTCAA GCAGATTGGC CTGTTTTGGG TAAGAAGCTT 
    CGCAAAGATA TGGCTCGTGT TAAGAAGGCT CTACCAAATG TTACTTCTGA GGAAGTTAAA 
    GAGTTTCAGA AGAACAAGAA AATGGTTTTG GATGGTATCG AACTTGTCGA GGGAGATTTA 
    CAAATTATTC GTTCTGTGGA GGTTAAGAAC GAGTTTTTGA AATCGAACAC TGATGGCATC 
    TGTATTGTTT TATTAGATAT AGAGATCGAT GCTCAGTTGC AAGCTGAAGG TTTGGCTCGC 
    GAAGTTATCA ACCGTGTTCA ACGTTTGCGC AAGAAGAGCA ATTTACAAGT TACGGACGAT 
    GTTCGTATGA CTTATAAAAT AAAAAACGAT ACTATTGGTC TCGAATCTGC TGTAGATTCC 
    AATGAAGCCT TATTTTCGAA GGTATTGAGA AGACCCATCG AAAAGGAAAC TGGTGCTGAC 
    GAGTCAAACA TAATTGCTTC CGAAGAACAA GACGTGCAAG GAGCTACCTT CCTTCTCAGT 
    CTTCTTAGGC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595569.1
    CDS145..3339
    Translation

    Target ORF information:

    RefSeq Version NM_001021464.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative isoleucine--tRNA(Ile) ligase Irs1 (irs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001021464.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCGTTTA ACGTTCCTAA AGAAGAGGAA AAAATCGTTG AGTTTTGGCG CGAAATCGAT 
    GCGTTCCATA CTCAGTTAAA ACTTTCTCAA GGTCGTCCTA CTTATACATT TTTCGATGGA 
    CCTCCATTCG CTACCGGTCG TCCTCATCAT GGTCATCTTT TGGCTTCTAC CATTAAGGAT 
    AGTGTCACCC GTTATGCTTG CTTGAAGGGT TATCACGTCG AGCGTAGATT CGGTTGGGAT 
    ACACATGGAC TTCCTGTTGA GCATGAAATC GACAAAAAGT TGGGTATTAC TGGAAGTGAT 
    GATGTTATGG CTATGGGTAT TGACAAATAC AATGCCGAAT GTCGTAAAAT TGTCATGACT 
    TATGCATCCG AATGGCGTGC AACAGTTGAA AGACTGGGCC GTTGGATTGA TTTTGATAAC 
    GATTATAAAA CGTTGTATCC TTCTTTCATG GAAAGTGTTT GGTGGGTTTT CAAAGAGTTG 
    CATACAAAAG GAAAAGTTTA CCGCGGATAT CGTGTTATGC CTTACTCGAC TGCATGTACA 
    ACACCTTTGA GTAACTTTGA AGCTCAACAA AACTATAAGG AAGTCCCAGA TCCGGCTATT 
    GTTGTTGCCT TTCAATCCAT TAGTGATCCT GAAGTTTCTT TCTTAGCTTG GACTACTACT 
    CCATGGACCT TGCCTTCTAA CCTTGCTTTG GCTGTTCACC CTGACTTACA GTACATAAAA 
    ATTTTGGACA AGGATTCTAA TAAGAAATAT ATCCTTATGG AGAGCTGCCT TGGCATCCTT 
    TACAAGAACC CAAAAAAGGC GAACTTTGAA ATTTTGGAAC GTTTCCAAGG AAAGGCCTTG 
    GATGGTCAAA AGTATGAACC TCTCTTCCCT TATTTCAAGA GCACTTTTGG CGAAAGAGCA 
    TTTAAATTAT ATTCTGCTGA CTACGTTGAA GAAGGCAGTG GTACTGGTAT CGTCCATCAA 
    GCTCCTGCTT TTGGTGAAGC AGATTATGAC GCTGCTTGGG CTGCTGGCAT CATCGATGCT 
    GATCACCAAC CTCCTTGCCC TGTTGATGAG CAAGGTTTGC TTACTTCGGA AATCACAGAC 
    TTCGCTGGCC AATATGTTAA AGACGCAGAT AAGGAAATTA TCCGCTCCTT GAAAGCATCT 
    GGCCATTTGG TTAAGCATAG TCAAATTTTC CATAGTTATC CATTTTGCTG GCGTTCCGAT 
    ACACCTCTAA TTTATCGTGC CGTTCCCAGT TGGTTTGTTC GCGTGAAGGA GATCACTAAT 
    GAGATGGTTG AAAATGTCAT GAGCACTCAC TGGGTCCCTC AAAACATTCG TGATAAACGT 
    TTTGCGAATT GGTTGAAGAA TGCTCGTGAC TGGAACATTT CCCGTAACAG ATACTGGGGT 
    ACCCCTATCC CACTTTGGGT TAGTGATGAC TACGAAGAAG TCGTTTGCAT TGGTTCCATT 
    AAGGAGCTTG AAGAACTTTC CGGCGTTTCT AATATTACAG ACATTCATCG TGACAGTATT 
    GATCATATCA CTATTCCTTC TAAAAAAGGT AAAGGAACAT TGCATCGTGT CAGTGAAGTA 
    TTTGACTGTT GGTTTGAATC TGGCAGCATG CCTTATGCTT CTCGTCATTA CCCTTTCGAA 
    AGAATTGAGG AATTCAAGCA TGGCTTCCCT GCTGACTTTA TTTCCGAGGG TGTTGATCAA 
    ACCCGTGGAT GGTTTTATAC TTTAACTGTT TTGGGAACTT TGTTATTCGA CAAAGCACCT 
    TATAAAAATG TAATTGTTAG TGGTCTGGTA ATGGCTGAGG ATGGTAAAAA AATGTCCAAA 
    CGTCTTAAAA ATTACCCTGA ACCAAATCTC ATCATTGAAA AATATGGTTC TGATGCCCTT 
    CGTTTGTATC TCATCAACTC TCCAGTTGTT CGTGCTGAGA TTCTTAAATT TAAGGAAGAT 
    GGTGTAAGAG AAGTTGTTAC TAGAGTTTTG ATTCCTTGGT GGAACAGTTA TAAGTTTTTT 
    GAGGCGCAAG CTGCTCTTTA CAAGAAGGTT ACTGGTAAAG ACTTTGTTTT CGATGATGCT 
    GCCACTTTGA GTTCCAATGT AATGGATCGC TGGATTTTAG CTCGTTGCCA GAGCTTGATT 
    GGATTTGTTG ATGAAGAAAT GAAACAATAC CGTTTGTACA CCGTGGTCCC TCAACTTCTC 
    GGTTTAATTG AGGAGATGAC TAACTGGTAT ATCCGGTTTA ACAGACGTCG TTTGAAGGGT 
    GAAGATGGTG AAATTGAAAC TATTAATGCT TTGAACGTTT TGTTTGAAGT CTTGTTCACG 
    TTAGTTCGTA TTATGGGCCC ATTCACCCCT TTCATTACTG AAAACATTTA TCAACACCTT 
    AGAAACTACA TGCCTATTGA CAAAAATGAA ATTTCACTTC GTTCAGTTCA TTTCCTTCCT 
    TTCCCTACTT ACAAGTCTGA GCTTGATGAT GAAACTGTTT TACGCCGTGT TAAGCGTATG 
    CAAACTATTA TCGAATTGGC GCGTTATGTT CGTGAGCAGA ATAATATTTC TTTGAAGACT 
    CCCTTAAAAA CCCTTATTGT TATTCTCACT AACGAAGAAT ACTTGGAAGA TGCTAAGCTC 
    TTAGAAAGAT ATATTGCAGA GGAGCTTAAC GTCCGTGAAG TAGTTTTCAC CTCCAATGAA 
    GAGAAGTATG GAGTCGTATA CAGCGTTCAA GCAGATTGGC CTGTTTTGGG TAAGAAGCTT 
    CGCAAAGATA TGGCTCGTGT TAAGAAGGCT CTACCAAATG TTACTTCTGA GGAAGTTAAA 
    GAGTTTCAGA AGAACAAGAA AATGGTTTTG GATGGTATCG AACTTGTCGA GGGAGATTTA 
    CAAATTATTC GTTCTGTGGA GGTTAAGAAC GAGTTTTTGA AATCGAACAC TGATGGCATC 
    TGTATTGTTT TATTAGATAT AGAGATCGAT GCTCAGTTGC AAGCTGAAGG TTTGGCTCGC 
    GAAGTTATCA ACCGTGTTCA ACGTTTGCGC AAGAAGAGCA ATTTACAAGT TACGGACGAT 
    GTTCGTATGA CTTATAAAAT AAAAAACGAT ACTATTGGTC TCGAATCTGC TGTAGATTCC 
    AATGAAGCCT TATTTTCGAA GGTATTGAGA AGACCCATCG AAAAGGAAAC TGGTGCTGAC 
    GAGTCAAACA TAATTGCTTC CGAAGAACAA GACGTGCAAG GAGCTACCTT CCTTCTCAGT 
    CTTCTTAGGC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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