IARS cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS
Entrez Gene ID	509885
Full Name	isoleucyl-tRNA synthetase
General protein information	Preferred Names isoleucyl-tRNA synthetase Names isoleucine--tRNA ligase, cytoplasmic isoleucine tRNA synthetase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005210426.4	XP_005210483.1	isoleucine--tRNA ligase, cytoplasmic isoform X1
NM_001101069.2	NP_001094539.1	isoleucine--tRNA ligase, cytoplasmic
XM_005210426.3	XP_005210483.1	isoleucine--tRNA ligase, cytoplasmic isoform X1
XM_024995578.1	XP_024851346.1	isoleucine--tRNA ligase, cytoplasmic isoform X2

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology
Bibliography

Related articles in PubMed

IARS mutation causes prenatal death in Japanese Black cattle.
Hirano T, Matsuhashi T, Takeda K, Hara H, Kobayashi N, Kita K, Sugimoto Y, Hanzawa K
Animal science journal = Nihon chikusan Gakkaiho87(9)1178-81(2016 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Its mutation causes prenatal death in Japanese Black cattle
Title: IARS mutation causes prenatal death in Japanese Black cattle.

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa237006	XM_024995578.1 Latest version!	Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa40687	NM_001101069.2 Latest version!	Bos taurus isoleucyl-tRNA synthetase 1 (IARS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa22284	XM_005210426.4 Latest version!	Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OBa22284	XM_005210426.3	Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS ( XM_024995578.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851346.1 Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa237006
Clone ID Related Accession (Same CDS sequence)	XM_024995578.1
Accession Version	XM_024995578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATACT CTACTGAATG GAAGTCTACT ATTACCAGAC TTGGCCGATG GATTGACTTT 
GACAATGACT ACAAAACTCT GTATCCACAA TTCATGGAAT CTGTTTGGTG GATCTTCAAA 
CAACTCTATG ATAAAGGCCT TGTTTATAGA GGTGTGAAAG TCATGCCCTT CTCCACTGCA 
TGCAATACTC CACTCTCCAA CTTTGAATCT CACCAGAATT ACAAGGATGT TCAAGATCCT 
TCAATATTTG TAACTTTCCC TTTGGAGGAA GATGAAAATA TTTCTTTGGT TGCCTGGACA 
ACTACTCCCT GGACTCTTCC TAGTAACCTT GCCCTGTGTG TTAATCCCGA CTTGCAATAT 
GTGAAGATTA AAGATTCTGT CAGAGGAAAA TTACTCATTT TAATGGAAGC CAGATTATCA 
GCCCTCTATA AATCGGAGAG TGACTATGAG ATCCTTGAAA GATTTCCTGG TGCCTCTCTC 
AGAGGCAAGA AATACCGGCC CCTGTTTGAC TATTTTGTAA AGTGTAAAGA GAGTGGTGCT 
TTTACTGTGC TTGTGGACAA CTATGTGAAA GAGGAAGAAG GCACTGGGAT TGTACACCAA 
GCTCCTTACT TTGGTGCTGA CGATTATCGG GTCTGTATGG ATTTCAACAT CATTCAGAAA 
GACTCTCTCC CTATTTGCCC TGTGGATGCC TCAGGCTGTT TTACAGCTGA AGTGACAGAT 
TTTGCAGGAC AGTATGTGAA GGATGCTGAC AAAAATATCA TCAAGACTCT AAAGGAACAA 
GGTCGACTTC TTGTTGCCAG CACCTTCACC CATAGCTACC CTTTTTGTTG GAGGTCAGAC 
ACTCCACTCA TTTACAAAGC AGTGCCTAGC TGGTTCGTTA GGGTGGAGCA CATGGTGGAT 
CAGCTGCTGA GGAACAATGA CCTGTGTTAC TGGGTCCCAG AGTTTGTGCG AGAAAAGCGA 
TTTGGAAATT GGCTGAAAGA TGCACGTGAC TGGGCAATTT CCAGAAACAG ATACTGGGGC 
ACTCCTATCC CACTGTGGGT CAGCAGTGAC TTCGAGGAGG TAGTATGTAT TGGGTCAATA 
GCAGAACTGG AAGAACTATC AGGAACAAAG ATCTCAGATC TCCACAGAGA GAGCATTGAC 
CATCTGACCA TTCCTTCACG CTGTGGAAAG GGATTGTTGC ATCGTGTCTC TGAAGTGTTT 
GACTGTTGGT TCGAGAGTGG CAGCATGCCA TATGCTCAAA TTCATTATCC ATTTGAAAAC 
AAGAGGGAGT TTGAGGATGC ATTTCCTGCA GACTTCATTG CTGAAGGCAT TGACCAAACC 
AGAGGATGGT TTTACACCTT GCTGGTGCTA GCCACATCTC TCTTTGGACG GCCACCATTC 
AAAAATGTGA TTGTGAATGG ACTTGTCCTG GCAAGTGATG GTCAAAAAAT GAGCAAACGC 
AAAAAGAATT ATCCAGACCC ACTTTCAGTA ATTGATAAAT ATGGTGCTGA TGCCCTCAGA 
TTATATCTGA TTAACTCCCC TGTGGTGAGA GCAGAAAACC TTCGCTTTAA GGAAGAGGGT 
GTCCGAGATG TCCTGAAGGA TGTACTGCTC CCTTGGTACA ATGCCTATCG CTTCTTCATC 
CAGAACGTCC TAAGGCTACA GAAGGAAGAA GAAGTGGAAT TCCTTTACAA CGAGAACATG 
GTTAAAGAAA GTACCAACAT CACAGACCGG TGGGTCCTGT CCTTTATGCA GTCGCTCGTT 
GGGTTCTTTG AGACCGAAAT GGCAGCTTAC AGGCTCTATA CTGTGGTTCC TCGCCTGGTC 
AAGTTTGTGG ATATTTTGAC CAATTGGTAT GTTCGGATGA ACCGTAGAAG ATTAAAGGGT 
GAAAATGGAG TAGAGGATTG TGTTAAGGCC CTGGAGACCT TGTTTAGTGT TCTGCTGTCT 
TTGTGCAGAC TTATGGCCCC CTATACCCCT TTTCTTACTG AATTGATGTA TCAGAATCTG 
AAGACACTGA TTGACCCTGT TTCTGTCCAG GACAAGGACA CATTCAGTAT TCACTACCTC 
ATGCTGCCCC ATGTTCGTGA GGAATTGATC GACAAGAAAA CTGAGAGTGC AGTATCTAGA 
ATGCAGTCTG TGATTGAACT TGGCCGAGTG ATTCGAGACC GCAAAACTAT TCCAATAAAG 
TATCCTTTGA AAGAAATTGT TGTTATCCAT CAAGATCAGG AAGCTCTTAA TGAAGTTAAG 
TCTTTGGAGA AATATATCAT TGAGGAATTG AATGTTCGAA AAGTTACATT ATCTACAGAT 
AAAAACAAGT ATGGCGTTCG CCTAAGGGCA GAGCCAGATC ACATGGTCCT GGGGAAGCGT 
CTGAAAGGAG CTTTCAAGAT GGTAATGACA TCCATCAAAC AGCTGAGCAA TGAGGAGCTG 
GAGCGGTTCC AGGAAAATGG GACCATTGTT GTGGAAGGCC ATGAATTGCA TGAAGAAGAT 
ATCCGTCTCA TATACACCTT TGATCAGGCA ACAGGTGGAA CAACACAGTT TGAAGCACAC 
TCGGATGCCC AGGTTTTGGT TCTCTTAGAT GTCACCCCTG ACCAGTCAAT GGTGGATGAA 
GGAATGGCTC GGGAAGTCAT CAATCGTATC CAGAAACTTC GCAAAAAGTG TAATCTGGTT 
CCAACTGATG AAATAACAGT TTATTATAAA GCAACTTCTG AGGGAAAATA TCTGAATAAT 
GTTATCGAAA GCCACACAGA CTTCATATTT GCTACCATAA AGTCTCCTTT GAAACCATAT 
CCAGTTCCTA TATCGGATGA AATACTTATT CAAGAAAAAA TGCAGTTAAA AGGGTCTGAC 
CTGGAAATTA CACTCACCAA AGGATCTTCC ACGCATGGCC CTGCTTGTGC ATATGTCAAT 
CTTAACATTT GTACAAATGG CAGTGAGCAA GGTGGAGTAT TACTTCTGGA AAATCCAAAA 
GGAGATAATA GGTTGGACCT TGTAAAACTG AAGAATGTTG TTACTAGCAT TTTTGGTGTA 
AAAAATACAG AGCTGGCTGT CCTCCTTGGT GAAACAGAAA TAAAAAACCA AACCAACTTA 
CTGAATCTTA GTGGGAAAAC ACTGTGTGTG ACTGCAGGAT CAGCGCTGTC TGTGGTGGAC 
AGTCCTAGTA CTCTTCTCTG TCAGTACATC AACCTACAGC TCTTGAATGC AGATCCACAA 
GAGTGTTTAA TGGGAACAGT AGGCACTCTT CTGCTTGAAA ATCCACTTGG ACAGAATGGG 
CTTACCCACC AAGGTCTTCT GTATGAAGCT GCCAAGATGT TTGGCCTCCG GAGCAGGAAG 
CTAAAGCTGT TTCTGAATGA GACTCAAACA GAAGAAATTA CAGAAGAAAT CCCTATGAAG 
ACTTTGAATA CGAAGACTGT GTATGTTTCT GTGTTACCAA CTACAGCAGA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851346.1
CDS	394..3810
Translation	MRYSTEWKSTITRLGRWIDFDNDYKTLYPQFMESVWWIFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENISLVAWTTTPWTLPSNLALCVNPDLQYVKIKDSVRGKLLILMEARLSALYKSESDYEILERFPGASLRGKKYRPLFDYFVKCKESGAFTVLVDNYVKEEEGTGIVHQAPYFGADDYRVCMDFNIIQKDSLPICPVDASGCFTAEVTDFAGQYVKDADKNIIKTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVDQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSSDFEEVVCIGSIAELEELSGTKISDLHRESIDHLTIPSRCGKGLLHRVSEVFDCWFESGSMPYAQIHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATSLFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPLSVIDKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEEVEFLYNENMVKESTNITDRWVLSFMQSLVGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGVEDCVKALETLFSVLLSLCRLMAPYTPFLTELMYQNLKTLIDPVSVQDKDTFSIHYLMLPHVREELIDKKTESAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDQEALNEVKSLEKYIIEELNVRKVTLSTDKNKYGVRLRAEPDHMVLGKRLKGAFKMVMTSIKQLSNEELERFQENGTIVVEGHELHEEDIRLIYTFDQATGGTTQFEAHSDAQVLVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTDFIFATIKSPLKPYPVPISDEILIQEKMQLKGSDLEITLTKGSSTHGPACAYVNLNICTNGSEQGGVLLLENPKGDNRLDLVKLKNVVTSIFGVKNTELAVLLGETEIKNQTNLLNLSGKTLCVTAGSALSVVDSPSTLLCQYINLQLLNADPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKMFGLRSRKLKLFLNETQTEEITEEIPMKTLNTKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_024995578.1
Organism	Bos taurus(cattle)
Definition	Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995578.1

ORF Insert Sequence:

ATGCGATACT CTACTGAATG GAAGTCTACT ATTACCAGAC TTGGCCGATG GATTGACTTT 
GACAATGACT ACAAAACTCT GTATCCACAA TTCATGGAAT CTGTTTGGTG GATCTTCAAA 
CAACTCTATG ATAAAGGCCT TGTTTATAGA GGTGTGAAAG TCATGCCCTT CTCCACTGCA 
TGCAATACTC CACTCTCCAA CTTTGAATCT CACCAGAATT ACAAGGATGT TCAAGATCCT 
TCAATATTTG TAACTTTCCC TTTGGAGGAA GATGAAAATA TTTCTTTGGT TGCCTGGACA 
ACTACTCCCT GGACTCTTCC TAGTAACCTT GCCCTGTGTG TTAATCCCGA CTTGCAATAT 
GTGAAGATTA AAGATTCTGT CAGAGGAAAA TTACTCATTT TAATGGAAGC CAGATTATCA 
GCCCTCTATA AATCGGAGAG TGACTATGAG ATCCTTGAAA GATTTCCTGG TGCCTCTCTC 
AGAGGCAAGA AATACCGGCC CCTGTTTGAC TATTTTGTAA AGTGTAAAGA GAGTGGTGCT 
TTTACTGTGC TTGTGGACAA CTATGTGAAA GAGGAAGAAG GCACTGGGAT TGTACACCAA 
GCTCCTTACT TTGGTGCTGA CGATTATCGG GTCTGTATGG ATTTCAACAT CATTCAGAAA 
GACTCTCTCC CTATTTGCCC TGTGGATGCC TCAGGCTGTT TTACAGCTGA AGTGACAGAT 
TTTGCAGGAC AGTATGTGAA GGATGCTGAC AAAAATATCA TCAAGACTCT AAAGGAACAA 
GGTCGACTTC TTGTTGCCAG CACCTTCACC CATAGCTACC CTTTTTGTTG GAGGTCAGAC 
ACTCCACTCA TTTACAAAGC AGTGCCTAGC TGGTTCGTTA GGGTGGAGCA CATGGTGGAT 
CAGCTGCTGA GGAACAATGA CCTGTGTTAC TGGGTCCCAG AGTTTGTGCG AGAAAAGCGA 
TTTGGAAATT GGCTGAAAGA TGCACGTGAC TGGGCAATTT CCAGAAACAG ATACTGGGGC 
ACTCCTATCC CACTGTGGGT CAGCAGTGAC TTCGAGGAGG TAGTATGTAT TGGGTCAATA 
GCAGAACTGG AAGAACTATC AGGAACAAAG ATCTCAGATC TCCACAGAGA GAGCATTGAC 
CATCTGACCA TTCCTTCACG CTGTGGAAAG GGATTGTTGC ATCGTGTCTC TGAAGTGTTT 
GACTGTTGGT TCGAGAGTGG CAGCATGCCA TATGCTCAAA TTCATTATCC ATTTGAAAAC 
AAGAGGGAGT TTGAGGATGC ATTTCCTGCA GACTTCATTG CTGAAGGCAT TGACCAAACC 
AGAGGATGGT TTTACACCTT GCTGGTGCTA GCCACATCTC TCTTTGGACG GCCACCATTC 
AAAAATGTGA TTGTGAATGG ACTTGTCCTG GCAAGTGATG GTCAAAAAAT GAGCAAACGC 
AAAAAGAATT ATCCAGACCC ACTTTCAGTA ATTGATAAAT ATGGTGCTGA TGCCCTCAGA 
TTATATCTGA TTAACTCCCC TGTGGTGAGA GCAGAAAACC TTCGCTTTAA GGAAGAGGGT 
GTCCGAGATG TCCTGAAGGA TGTACTGCTC CCTTGGTACA ATGCCTATCG CTTCTTCATC 
CAGAACGTCC TAAGGCTACA GAAGGAAGAA GAAGTGGAAT TCCTTTACAA CGAGAACATG 
GTTAAAGAAA GTACCAACAT CACAGACCGG TGGGTCCTGT CCTTTATGCA GTCGCTCGTT 
GGGTTCTTTG AGACCGAAAT GGCAGCTTAC AGGCTCTATA CTGTGGTTCC TCGCCTGGTC 
AAGTTTGTGG ATATTTTGAC CAATTGGTAT GTTCGGATGA ACCGTAGAAG ATTAAAGGGT 
GAAAATGGAG TAGAGGATTG TGTTAAGGCC CTGGAGACCT TGTTTAGTGT TCTGCTGTCT 
TTGTGCAGAC TTATGGCCCC CTATACCCCT TTTCTTACTG AATTGATGTA TCAGAATCTG 
AAGACACTGA TTGACCCTGT TTCTGTCCAG GACAAGGACA CATTCAGTAT TCACTACCTC 
ATGCTGCCCC ATGTTCGTGA GGAATTGATC GACAAGAAAA CTGAGAGTGC AGTATCTAGA 
ATGCAGTCTG TGATTGAACT TGGCCGAGTG ATTCGAGACC GCAAAACTAT TCCAATAAAG 
TATCCTTTGA AAGAAATTGT TGTTATCCAT CAAGATCAGG AAGCTCTTAA TGAAGTTAAG 
TCTTTGGAGA AATATATCAT TGAGGAATTG AATGTTCGAA AAGTTACATT ATCTACAGAT 
AAAAACAAGT ATGGCGTTCG CCTAAGGGCA GAGCCAGATC ACATGGTCCT GGGGAAGCGT 
CTGAAAGGAG CTTTCAAGAT GGTAATGACA TCCATCAAAC AGCTGAGCAA TGAGGAGCTG 
GAGCGGTTCC AGGAAAATGG GACCATTGTT GTGGAAGGCC ATGAATTGCA TGAAGAAGAT 
ATCCGTCTCA TATACACCTT TGATCAGGCA ACAGGTGGAA CAACACAGTT TGAAGCACAC 
TCGGATGCCC AGGTTTTGGT TCTCTTAGAT GTCACCCCTG ACCAGTCAAT GGTGGATGAA 
GGAATGGCTC GGGAAGTCAT CAATCGTATC CAGAAACTTC GCAAAAAGTG TAATCTGGTT 
CCAACTGATG AAATAACAGT TTATTATAAA GCAACTTCTG AGGGAAAATA TCTGAATAAT 
GTTATCGAAA GCCACACAGA CTTCATATTT GCTACCATAA AGTCTCCTTT GAAACCATAT 
CCAGTTCCTA TATCGGATGA AATACTTATT CAAGAAAAAA TGCAGTTAAA AGGGTCTGAC 
CTGGAAATTA CACTCACCAA AGGATCTTCC ACGCATGGCC CTGCTTGTGC ATATGTCAAT 
CTTAACATTT GTACAAATGG CAGTGAGCAA GGTGGAGTAT TACTTCTGGA AAATCCAAAA 
GGAGATAATA GGTTGGACCT TGTAAAACTG AAGAATGTTG TTACTAGCAT TTTTGGTGTA 
AAAAATACAG AGCTGGCTGT CCTCCTTGGT GAAACAGAAA TAAAAAACCA AACCAACTTA 
CTGAATCTTA GTGGGAAAAC ACTGTGTGTG ACTGCAGGAT CAGCGCTGTC TGTGGTGGAC 
AGTCCTAGTA CTCTTCTCTG TCAGTACATC AACCTACAGC TCTTGAATGC AGATCCACAA 
GAGTGTTTAA TGGGAACAGT AGGCACTCTT CTGCTTGAAA ATCCACTTGG ACAGAATGGG 
CTTACCCACC AAGGTCTTCT GTATGAAGCT GCCAAGATGT TTGGCCTCCG GAGCAGGAAG 
CTAAAGCTGT TTCTGAATGA GACTCAAACA GAAGAAATTA CAGAAGAAAT CCCTATGAAG 
ACTTTGAATA CGAAGACTGT GTATGTTTCT GTGTTACCAA CTACAGCAGA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( NM_001101069.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001094539.1 Bos taurus isoleucyl-tRNA synthetase 1 (IARS1), mRNA.

Price & Availability↑

CloneID	OBa40687
Clone ID Related Accession (Same CDS sequence)	NM_001101069.2
Accession Version	NM_001101069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-30
Organism	Bos taurus(cattle)
Product	isoleucine--tRNA ligase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151484.1. On Aug 31, 2012 this sequence version replaced NM_001101069.1. ##Evidence-Data-START## Transcript exon combination :: BC151484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA 
TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC 
AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC 
CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG 
TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG 
GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC 
GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA 
GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG 
ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC 
ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT 
GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT 
CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT 
TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG 
CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC 
AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT 
ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT 
CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA 
CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT 
AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC 
AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG 
CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT 
CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA 
AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT 
GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG 
TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT 
ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT 
AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC 
AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG 
TTTGAAGCAC ACTCAGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG 
TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA 
TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT 
TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA 
AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG 
GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC 
ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC 
CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG 
TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT 
GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT 
GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA 
ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094539.1
CDS	71..3859
Translation	MVQQVPENISFPAEEEKILQFWSDFNCFQECLKQSKHRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKLGIAEYNSQCRAIVMRYSTEWKSTITRLGRWIDFDNDYKTLYPQFMESVWWIFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENISLVAWTTTPWTLPSNLALCVNPDLQYVKIKDSVRGKLLILMEARLSALYKSESDYEILERFPGASLRGKKYRPLFDYFVKCKESGAFTVLVDNYVKEEEGTGIVHQAPYFGADDYRVCMDFNIIQKDSLPICPVDASGCFTAEVTDFAGQYVKDADKNIIKTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVDQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSSDFEEVVCIGSIAELEELSGTKISDLHRESIDHLTIPSRCGKGLLHRVSEVFDCWFESGSMPYAQIHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATSLFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPLSVIDKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEEVEFLYNENMVKESTNITDRWVLSFMQSLVGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGVEDCVKALETLFSVLLSLCRLMAPYTPFLTELMYQNLKTLIDPVSVQDKDTFSIHYLMLPHVREELIDKKTESAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDQEALNEVKSLEKYIIEELNVRKVTLSTDKNKYGVRLRAEPDHMVLGKRLKGAFKMVMTSIKQLSNEELERFQENGTIVVEGHELHEEDIRLIYTFDQATGGTTQFEAHSDAQVLVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTDFIFATIKSPLKPYPVPISDEILIQEKMQLKGSDLEITLTKGSSTHGPACAYVNLNICTNGSEQGGVLLLENPKGDNRLDLVKLKNVVTSIFGVKNTELAVLLGETEIKNQTNLLNLSGKTLCVTAGSALSVVDSPSTLLCQYINLQLLNADPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKMFGLRSRKLKLFLNETQTEEITEEIPMKTLNTKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	NM_001101069.2
Organism	Bos taurus(cattle)
Definition	Bos taurus isoleucyl-tRNA synthetase 1 (IARS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101069.2

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA 
TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC 
AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC 
CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG 
TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG 
GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC 
GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA 
GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG 
ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC 
ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT 
GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT 
CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT 
TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG 
CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC 
AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT 
ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT 
CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA 
CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT 
AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC 
AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG 
CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT 
CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA 
AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT 
GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG 
TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT 
ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT 
AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC 
AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG 
TTTGAAGCAC ACTCAGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG 
TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA 
TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT 
TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA 
AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG 
GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC 
ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC 
CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG 
TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT 
GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT 
GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA 
ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_005210426.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210483.1 Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

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CloneID	OBa22284
Clone ID Related Accession (Same CDS sequence)	XM_005210426.4 , XM_005210426.3
Accession Version	XM_005210426.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210426.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA 
TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC 
AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC 
CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG 
TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG 
GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC 
GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA 
GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG 
ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC 
ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT 
GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT 
CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT 
TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG 
CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC 
AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT 
ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT 
CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA 
CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT 
AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC 
AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG 
CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT 
CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA 
AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT 
GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG 
TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT 
ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT 
AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC 
AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG 
TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG 
TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA 
TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT 
TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA 
AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG 
GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC 
ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC 
CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG 
TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT 
GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT 
GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA 
ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210483.1
CDS	152..3940
Translation	MVQQVPENISFPAEEEKILQFWSDFNCFQECLKQSKHRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKLGIAEYNSQCRAIVMRYSTEWKSTITRLGRWIDFDNDYKTLYPQFMESVWWIFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENISLVAWTTTPWTLPSNLALCVNPDLQYVKIKDSVRGKLLILMEARLSALYKSESDYEILERFPGASLRGKKYRPLFDYFVKCKESGAFTVLVDNYVKEEEGTGIVHQAPYFGADDYRVCMDFNIIQKDSLPICPVDASGCFTAEVTDFAGQYVKDADKNIIKTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVDQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSSDFEEVVCIGSIAELEELSGTKISDLHRESIDHLTIPSRCGKGLLHRVSEVFDCWFESGSMPYAQIHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATSLFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPLSVIDKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEEVEFLYNENMVKESTNITDRWVLSFMQSLVGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGVEDCVKALETLFSVLLSLCRLMAPYTPFLTELMYQNLKTLIDPVSVQDKDTFSIHYLMLPHVREELIDKKTESAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDQEALNEVKSLEKYIIEELNVRKVTLSTDKNKYGVRLRAEPDHMVLGKRLKGAFKMVMTSIKQLSNEELERFQENGTIVVEGHELHEEDIRLIYTFDQATGGTTQFEAHSDAQVLVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTDFIFATIKSPLKPYPVPISDEILIQEKMQLKGSDLEITLTKGSSTHGPACAYVNLNICTNGSEQGGVLLLENPKGDNRLDLVKLKNVVTSIFGVKNTELAVLLGETEIKNQTNLLNLSGKTLCVTAGSALSVVDSPSTLLCQYINLQLLNADPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKMFGLRSRKLKLFLNETQTEEITEEIPMKTLNTKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_005210426.4
Organism	Bos taurus(cattle)
Definition	Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210426.4

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA 
TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC 
AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC 
CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG 
TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG 
GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC 
GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA 
GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG 
ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC 
ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT 
GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT 
CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT 
TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG 
CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC 
AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT 
ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT 
CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA 
CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT 
AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC 
AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG 
CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT 
CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA 
AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT 
GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG 
TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT 
ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT 
AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC 
AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG 
TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG 
TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA 
TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT 
TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA 
AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG 
GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC 
ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC 
CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG 
TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT 
GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT 
GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA 
ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_005210426.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210483.1 Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

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CloneID	OBa22284
Clone ID Related Accession (Same CDS sequence)	XM_005210426.4 , XM_005210426.3
Accession Version	XM_005210426.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA 
TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC 
AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC 
CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG 
TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG 
GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC 
GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA 
GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG 
ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC 
ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT 
GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT 
CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT 
TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG 
CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC 
AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT 
ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT 
CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA 
CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT 
AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC 
AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG 
CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT 
CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA 
AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT 
GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG 
TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT 
ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT 
AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC 
AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG 
TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG 
TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA 
TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT 
TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA 
AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG 
GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC 
ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC 
CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG 
TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT 
GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT 
GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA 
ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210483.1
CDS	127..3915
Translation	MVQQVPENISFPAEEEKILQFWSDFNCFQECLKQSKHRPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKLGIAEYNSQCRAIVMRYSTEWKSTITRLGRWIDFDNDYKTLYPQFMESVWWIFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSIFVTFPLEEDENISLVAWTTTPWTLPSNLALCVNPDLQYVKIKDSVRGKLLILMEARLSALYKSESDYEILERFPGASLRGKKYRPLFDYFVKCKESGAFTVLVDNYVKEEEGTGIVHQAPYFGADDYRVCMDFNIIQKDSLPICPVDASGCFTAEVTDFAGQYVKDADKNIIKTLKEQGRLLVASTFTHSYPFCWRSDTPLIYKAVPSWFVRVEHMVDQLLRNNDLCYWVPEFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSSDFEEVVCIGSIAELEELSGTKISDLHRESIDHLTIPSRCGKGLLHRVSEVFDCWFESGSMPYAQIHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATSLFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPLSVIDKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFFIQNVLRLQKEEEVEFLYNENMVKESTNITDRWVLSFMQSLVGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGVEDCVKALETLFSVLLSLCRLMAPYTPFLTELMYQNLKTLIDPVSVQDKDTFSIHYLMLPHVREELIDKKTESAVSRMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDQEALNEVKSLEKYIIEELNVRKVTLSTDKNKYGVRLRAEPDHMVLGKRLKGAFKMVMTSIKQLSNEELERFQENGTIVVEGHELHEEDIRLIYTFDQATGGTTQFEAHSDAQVLVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKATSEGKYLNNVIESHTDFIFATIKSPLKPYPVPISDEILIQEKMQLKGSDLEITLTKGSSTHGPACAYVNLNICTNGSEQGGVLLLENPKGDNRLDLVKLKNVVTSIFGVKNTELAVLLGETEIKNQTNLLNLSGKTLCVTAGSALSVVDSPSTLLCQYINLQLLNADPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKMFGLRSRKLKLFLNETQTEEITEEIPMKTLNTKTVYVSVLPTTADF

Target ORF information:

RefSeq Version	XM_005210426.3
Organism	Bos taurus(cattle)
Definition	Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210426.3

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA 
TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT 
GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC 
AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG 
GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC 
CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA 
TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG 
TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC 
TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT 
GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG 
GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC 
GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA 
GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT 
GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA 
GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG 
ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC 
ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT 
GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT 
CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT 
TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG 
CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG 
CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC 
AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT 
ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA 
GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC 
TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT 
CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA 
CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA 
ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT 
GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT 
AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT 
CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC 
AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG 
CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT 
CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA 
AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT 
GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG 
TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT 
ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT 
GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT 
ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT 
AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA 
TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC 
CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC 
AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG 
CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG 
TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA 
ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG 
TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA 
TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT 
TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA 
AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT 
GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG 
GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC 
ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC 
CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG 
TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT 
GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT 
GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC 
CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA 
ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA 
GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

IARS mutation causes prenatal death in Japanese Black cattle.
Animal science journal = Nihon chikusan Gakkaiho87(9)1178-81(2016 Sep)
Hirano T,Matsuhashi T,Takeda K,Hara H,Kobayashi N,Kita K,Sugimoto Y,Hanzawa K

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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IARS cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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