IARS cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS
Entrez Gene ID	415957
Full Name	isoleucyl-tRNA synthetase
General protein information	Preferred Names isoleucyl-tRNA synthetase Names isoleucyl-tRNA synthetase, cytoplasmic isoleucine--tRNA ligase, cytoplasmic isoleucine-tRNA synthetase
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_414300.4	XP_414300.3	isoleucine--tRNA ligase, cytoplasmic isoform X1
XM_015293480.2	XP_015148966.1	isoleucine--tRNA ligase, cytoplasmic isoform X2
XM_414300.5	XP_414300.3	isoleucine--tRNA ligase, cytoplasmic isoform X1
XM_015293480.1	XP_015148966.1	isoleucine--tRNA ligase, cytoplasmic isoform X2
XM_015293481.2	XP_015148967.1	isoleucine--tRNA ligase, cytoplasmic isoform X2
XM_015293481.1	XP_015148967.1	isoleucine--tRNA ligase, cytoplasmic isoform X2

Pathways from BioSystems

Homologies

	IARS	XP_001107299.2
Kluyveromyces lactis	KLLA0E20549g	XP_454879.1
Eremothecium gossypii	AGOS_AER394W	NP_985249.2
Magnaporthe oryzae (rice blast fungus)	MGG_03220	XP_003716759.1
Mus musculus (house mouse)	Iars	NP_742012.2
Gallus gallus (chicken)	IARS	XP_414300.3
Danio rerio (zebrafish)	iars	NP_956190.1
Saccharomyces cerevisiae (baker's yeast)	ILS1	NP_009477.1
Schizosaccharomyces pombe (fission yeast)	irs1	NP_595569.1
Rattus norvegicus (Norway rat)	Iars	NP_001094042.1
Homo sapiens (human)	IARS	NP_038203.2
Drosophila melanogaster (fruit fly)	Aats-ile	NP_788565.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002101	XP_320939.3
Neurospora crassa	NCU03575	XP_956014.1
Arabidopsis thaliana (thale cress)	AT4G10320	NP_192770.2
Xenopus tropicalis (tropical clawed frog)	iars	NP_001090690.1
Pan troglodytes (chimpanzee)	IARS	XP_003312212.1
Canis lupus familiaris (dog)	IARS	XP_541332.3
Bos taurus (cattle)	IARS	NP_001094539.1
Caenorhabditis elegans (roundworm)	iars-1	NP_501914.1

Gene Ontology

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa41720	XM_015293480.2 Latest version!	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41720	XM_015293481.2 Latest version!	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa14086	XM_414300.5 Latest version!	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OGa14086	XM_414300.4	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OGa41720	XM_015293480.1	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41720	XM_015293481.1	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS ( XM_015293480.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148966.1 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa41720
Clone ID Related Accession (Same CDS sequence)	XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
Accession Version	XM_015293480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148966.1
CDS	158..3955
Translation	MVQQVPENISFPNEEEKILELWKKLNCFQECLKQSKNRPRFNFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNNQCRGIVMRYSKEWEFNVTRLGRWIDFENDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVSFPLVEDASVSLVAWTTTPWTLPSNLALCVNPELQYVKLRDKATGKIYILMESRLIALYKSDSEYEILDRFPGIVLKGKKYKPLFEYFIQCKDQGAFTVLVDSYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDVSGCFTAEVTDFAGQYVKDADKNIIKLLKEKGRLVHSSSYKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNAQCYWVPDFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGVKVTDLHRESIDHLTIPSRCGKGVLRRVPEVFDCWFESGSMPYAQVHYPFENKKELEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPMAIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDILKDVFLPWYNAYRFLVQNVHILQHKDEGREFLYNENTVKESNNIMDKWILSFTQSLIQFFKAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLFSMCRLMAPYTPFITELMYQNLKTLIDPASVQEKNTESIHYLMLPPVREDLIDRKIESAVSCLQSVIELGRVIRDRKTIPVKYPLKEVVVIHQDPEALENIRSLEKYILEELNVRQVTLSTNKDKYGVRLRAEPDHMVLGKRLKGAFKPVMAAIKELKSEQLEEFQETGTIVVEGHELHGEDLRLMYTFDQVAGGSAQFEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYRSHPEGDYLDTVIKEHTDFIFATIKAALKPYPVPTSKEVLIQETTQLKGSELEITLVRGGLCERVGPACSYVNLKVCVNGTEQDGVLLLENPKGDNTLNLTGLVDAVSCIFGLKNSKLTVFNGKTELINKTDLLSLSGKTLHVTTGSAPALINSPDALLCQYINLQLVNAKPQECQKGTVGTLLMENPVGQNGLTYHGLLHETAKVFGLRSRRLKLFLDEAETQEITKDISMKNLNMKTVYVSVIPTTAEC

Target ORF information:

RefSeq Version	XM_015293480.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293480.2

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_015293480.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148966.1 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa41720
Clone ID Related Accession (Same CDS sequence)	XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
Accession Version	XM_015293480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148966.1
CDS	182..3979
Translation	MVQQVPENISFPNEEEKILELWKKLNCFQECLKQSKNRPRFNFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNNQCRGIVMRYSKEWEFNVTRLGRWIDFENDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVSFPLVEDASVSLVAWTTTPWTLPSNLALCVNPELQYVKLRDKATGKIYILMESRLIALYKSDSEYEILDRFPGIVLKGKKYKPLFEYFIQCKDQGAFTVLVDSYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDVSGCFTAEVTDFAGQYVKDADKNIIKLLKEKGRLVHSSSYKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNAQCYWVPDFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGVKVTDLHRESIDHLTIPSRCGKGVLRRVPEVFDCWFESGSMPYAQVHYPFENKKELEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPMAIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDILKDVFLPWYNAYRFLVQNVHILQHKDEGREFLYNENTVKESNNIMDKWILSFTQSLIQFFKAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLFSMCRLMAPYTPFITELMYQNLKTLIDPASVQEKNTESIHYLMLPPVREDLIDRKIESAVSCLQSVIELGRVIRDRKTIPVKYPLKEVVVIHQDPEALENIRSLEKYILEELNVRQVTLSTNKDKYGVRLRAEPDHMVLGKRLKGAFKPVMAAIKELKSEQLEEFQETGTIVVEGHELHGEDLRLMYTFDQVAGGSAQFEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYRSHPEGDYLDTVIKEHTDFIFATIKAALKPYPVPTSKEVLIQETTQLKGSELEITLVRGGLCERVGPACSYVNLKVCVNGTEQDGVLLLENPKGDNTLNLTGLVDAVSCIFGLKNSKLTVFNGKTELINKTDLLSLSGKTLHVTTGSAPALINSPDALLCQYINLQLVNAKPQECQKGTVGTLLMENPVGQNGLTYHGLLHETAKVFGLRSRRLKLFLDEAETQEITKDISMKNLNMKTVYVSVIPTTAEC

Target ORF information:

RefSeq Version	XM_015293480.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293480.1

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_015293481.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148967.1 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

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CloneID	OGa41720
Clone ID Related Accession (Same CDS sequence)	XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
Accession Version	XM_015293481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148967.1
CDS	113..3910
Translation	MVQQVPENISFPNEEEKILELWKKLNCFQECLKQSKNRPRFNFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNNQCRGIVMRYSKEWEFNVTRLGRWIDFENDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVSFPLVEDASVSLVAWTTTPWTLPSNLALCVNPELQYVKLRDKATGKIYILMESRLIALYKSDSEYEILDRFPGIVLKGKKYKPLFEYFIQCKDQGAFTVLVDSYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDVSGCFTAEVTDFAGQYVKDADKNIIKLLKEKGRLVHSSSYKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNAQCYWVPDFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGVKVTDLHRESIDHLTIPSRCGKGVLRRVPEVFDCWFESGSMPYAQVHYPFENKKELEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPMAIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDILKDVFLPWYNAYRFLVQNVHILQHKDEGREFLYNENTVKESNNIMDKWILSFTQSLIQFFKAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLFSMCRLMAPYTPFITELMYQNLKTLIDPASVQEKNTESIHYLMLPPVREDLIDRKIESAVSCLQSVIELGRVIRDRKTIPVKYPLKEVVVIHQDPEALENIRSLEKYILEELNVRQVTLSTNKDKYGVRLRAEPDHMVLGKRLKGAFKPVMAAIKELKSEQLEEFQETGTIVVEGHELHGEDLRLMYTFDQVAGGSAQFEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYRSHPEGDYLDTVIKEHTDFIFATIKAALKPYPVPTSKEVLIQETTQLKGSELEITLVRGGLCERVGPACSYVNLKVCVNGTEQDGVLLLENPKGDNTLNLTGLVDAVSCIFGLKNSKLTVFNGKTELINKTDLLSLSGKTLHVTTGSAPALINSPDALLCQYINLQLVNAKPQECQKGTVGTLLMENPVGQNGLTYHGLLHETAKVFGLRSRRLKLFLDEAETQEITKDISMKNLNMKTVYVSVIPTTAEC

Target ORF information:

RefSeq Version	XM_015293481.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293481.2

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_015293481.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148967.1 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa41720
Clone ID Related Accession (Same CDS sequence)	XM_015293480.2 , XM_015293480.1 , XM_015293481.2 , XM_015293481.1
Accession Version	XM_015293481.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148967.1
CDS	178..3975
Translation	MVQQVPENISFPNEEEKILELWKKLNCFQECLKQSKNRPRFNFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNNQCRGIVMRYSKEWEFNVTRLGRWIDFENDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVSFPLVEDASVSLVAWTTTPWTLPSNLALCVNPELQYVKLRDKATGKIYILMESRLIALYKSDSEYEILDRFPGIVLKGKKYKPLFEYFIQCKDQGAFTVLVDSYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDVSGCFTAEVTDFAGQYVKDADKNIIKLLKEKGRLVHSSSYKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNAQCYWVPDFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGVKVTDLHRESIDHLTIPSRCGKGVLRRVPEVFDCWFESGSMPYAQVHYPFENKKELEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPMAIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDILKDVFLPWYNAYRFLVQNVHILQHKDEGREFLYNENTVKESNNIMDKWILSFTQSLIQFFKAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLFSMCRLMAPYTPFITELMYQNLKTLIDPASVQEKNTESIHYLMLPPVREDLIDRKIESAVSCLQSVIELGRVIRDRKTIPVKYPLKEVVVIHQDPEALENIRSLEKYILEELNVRQVTLSTNKDKYGVRLRAEPDHMVLGKRLKGAFKPVMAAIKELKSEQLEEFQETGTIVVEGHELHGEDLRLMYTFDQVAGGSAQFEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYRSHPEGDYLDTVIKEHTDFIFATIKAALKPYPVPTSKEVLIQETTQLKGSELEITLVRGGLCERVGPACSYVNLKVCVNGTEQDGVLLLENPKGDNTLNLTGLVDAVSCIFGLKNSKLTVFNGKTELINKTDLLSLSGKTLHVTTGSAPALINSPDALLCQYINLQLVNAKPQECQKGTVGTLLMENPVGQNGLTYHGLLHETAKVFGLRSRRLKLFLDEAETQEITKDISMKNLNMKTVYVSVIPTTAEC

Target ORF information:

RefSeq Version	XM_015293481.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293481.1

ORF Insert Sequence:

ATGGTTCAAC AAGTTCCAGA AAACATAAGT TTTCCAAATG AGGAAGAGAA GATACTGGAG 
CTCTGGAAGA AGCTTAATTG TTTCCAGGAA TGCCTAAAGC AGTCAAAGAA TAGACCTAGG 
TTTAACTTCT ATGATGGCCC TCCATTTGCT ACCGGGCTTC CGCACTATGG CCATATTCTT 
GCAGGAACCA TTAAAGACAT AGTTACCAGA TTTGCTCACC AGAGCGGTTT TCATGTTGAC 
AGAAGATTTG GATGGGATTG CCATGGCCTA CCTGTGGAAT ATGAGATTGA CAAGACCTTA 
GGCATCAAAG GGCCAGAGGA TGTGGCAAAG ATGGGGATTG CAGAATACAA CAACCAGTGC 
AGAGGAATTG TGATGAGATA CTCAAAGGAA TGGGAGTTCA ACGTTACCAG ATTAGGACGC 
TGGATTGATT TTGAAAATGA CTATAAAACA CTTTATCCTG AATTCATGGA GTCTGTATGG 
TGGGTTTTCA AGCAGCTTTA TGACAAGGGA CTGGTATACA GAGGTGTTAA AGTCATGCCT 
TTTTCTACTG CTTGCAATAC CCCATTGTCA AATTTTGAGT CCCATCAGAA TTACAAGGAT 
GTTCAAGACC CTTCGGTGAT TGTGAGTTTC CCACTGGTTG AAGATGCAAG TGTCTCTCTT 
GTTGCTTGGA CCACTACACC TTGGACTTTA CCTAGCAATC TGGCCCTTTG TGTTAACCCA 
GAACTACAAT ATGTGAAATT GAGAGATAAG GCTACAGGAA AGATTTACAT CTTGATGGAG 
TCCAGGCTGA TAGCACTATA CAAATCTGAC AGTGAATATG AGATACTGGA CAGGTTTCCT 
GGCATTGTTC TGAAAGGCAA GAAGTATAAA CCACTCTTTG AGTATTTTAT TCAGTGCAAA 
GATCAAGGTG CCTTTACTGT GCTAGTAGAC AGTTATGTGA AGGAGGAAGA AGGCACAGGT 
GTTGTTCATC AAGCCCCCTA TTTTGGTGCT GACGATTACA GAGTCTGTAT GGATTTTAAT 
ATTATTCAGA AAGATTCTGT CCCAGTTTGT CCTGTTGATG TATCAGGCTG CTTCACAGCT 
GAAGTGACCG ACTTTGCTGG GCAGTATGTG AAGGATGCAG ATAAGAACAT CATTAAATTA 
CTGAAGGAGA AGGGCAGACT GGTTCACAGC AGTAGTTACA AACATAGCTA TCCGTTCTGC 
TGGAGGTCAG ATACTCCGTT GATATACAAA GCTGTGCCAA GCTGGTTTGT AAGAGTAGAG 
CATATGGTGG AGAAGCTACT GGAGAATAAT GCACAGTGCT ACTGGGTTCC GGATTTTGTG 
AGGGAGAAGC GTTTTGGGAA CTGGCTAAAA GATGCACGAG ATTGGGCTAT ATCTCGAAAC 
CGATACTGGG GAACACCCAT CCCACTGTGG GTCAGTGATG ATTTTGAAGA GGTAGTTTGT 
ATTGGTTCAA TGGCGGAACT GGAAGAGCTG TCAGGAGTGA AAGTCACTGA TCTCCATCGG 
GAAAGTATTG ATCATCTAAC CATCCCTTCA CGCTGCGGCA AAGGCGTTTT GCGCCGGGTC 
CCTGAAGTTT TTGATTGCTG GTTTGAGAGT GGAAGCATGC CTTATGCACA AGTGCATTAT 
CCGTTTGAAA ACAAAAAAGA ACTTGAAGAT GCTTTTCCAG CTGACTTCAT TGCTGAAGGC 
ATTGACCAAA CCCGAGGATG GTTCTACACT TTGCTGGTGC TGTCCACTGC TCTTTTTGGA 
AGACCTCCTT TCAAGAATGT AATTGTAAAT GGCCTAGTTC TTGCCAGTGA TGGACAAAAA 
ATGAGCAAAA GGAAGAAGAA TTATCCTGAT CCAATGGCTA TTGTAAATAG TTATGGAGCT 
GATGCACTAA GGTTATACCT TATCAATTCT CCAGTGGTAA GAGCGGAAAA CTTGAGATTC 
AAGGAGGAAG GAGTAAGGGA CATACTGAAG GATGTCTTCC TGCCCTGGTA CAATGCATAT 
CGCTTTCTAG TTCAGAACGT TCACATCCTG CAACACAAGG ATGAGGGAAG AGAATTCCTT 
TACAATGAGA ACACAGTTAA GGAGAGCAAT AACATCATGG ATAAATGGAT TCTTTCTTTC 
ACCCAATCGC TCATTCAGTT CTTCAAAGCT GAAATGGCAG CTTACAGGCT GTACACGGTA 
GTCCCTCGGT TAGTGAAGTT TGTAGACATT CTGACCAACT GGTATGTGAG AATGAATCGC 
AGAAGACTGA AGGGCGAGAA TGGGACTGAG GACTGCATTA TGGCCCTGGA AACCTTGTTC 
AGTGTTTTGT TTTCCATGTG CAGACTTATG GCTCCGTATA CTCCATTTAT CACTGAACTG 
ATGTACCAGA ATCTGAAGAC GCTTATTGAT CCAGCCTCAG TTCAGGAGAA GAATACTGAA 
AGCATTCACT ACCTCATGCT TCCCCCAGTG AGGGAGGATC TGATCGACAG AAAAATTGAA 
AGTGCTGTTT CTTGTTTGCA GTCTGTTATT GAGCTTGGAC GAGTAATCAG AGACAGAAAA 
ACCATTCCAG TAAAGTATCC TTTGAAAGAA GTAGTTGTTA TCCATCAAGA CCCAGAGGCT 
CTGGAAAACA TCAGGTCTCT AGAGAAGTAC ATACTTGAGG AGCTGAACGT ACGCCAGGTG 
ACGTTATCCA CGAATAAAGA TAAATATGGA GTCAGGCTGA GAGCAGAACC GGATCACATG 
GTGCTTGGGA AGCGTTTGAA AGGTGCATTT AAGCCTGTCA TGGCTGCAAT CAAAGAGCTG 
AAGAGTGAGC AGCTGGAGGA ATTCCAAGAG ACAGGCACCA TTGTTGTAGA AGGACACGAG 
CTCCATGGGG AGGATCTTCG TCTCATGTAT ACTTTTGATC AGGTTGCAGG AGGATCTGCT 
CAGTTTGAGG CACATTCTGA TGCTCAGGTT TTAGTTCTGC TGGATGTTAC CCCAGACCAG 
TCTATGGTGG ATGAAGGAGT GGCCCGGGAA GTGATCAACC GCATTCAGAA GCTTCGCAAA 
AAGCGAAATC TAGTTCCCAC AGATGAGATT ACAGTGTACT ACAGATCACA TCCAGAAGGT 
GATTACTTGG ATACTGTCAT TAAGGAGCAT ACAGATTTCA TTTTTGCAAC AATAAAGGCT 
GCCCTGAAGC CTTACCCGGT TCCTACCTCA AAAGAAGTTC TCATCCAGGA GACCACTCAG 
CTGAAGGGAT CGGAGCTAGA AATTACACTT GTCAGAGGTG GTTTGTGTGA GCGTGTTGGT 
CCAGCCTGTT CCTACGTCAA CCTCAAAGTC TGTGTTAATG GGACAGAACA AGATGGTGTG 
TTGCTGCTGG AGAATCCAAA AGGAGATAAT ACGTTGAACC TCACTGGACT TGTCGATGCA 
GTCTCCTGCA TTTTTGGACT TAAAAATTCA AAACTGACAG TTTTTAATGG AAAAACAGAA 
CTGATAAACA AAACTGACTT GCTTAGTCTC AGTGGAAAGA CTCTGCATGT GACAACGGGG 
TCAGCACCTG CTCTCATCAA CTCTCCTGAT GCTCTTCTCT GCCAGTATAT CAACTTGCAG 
TTAGTAAATG CAAAACCACA AGAGTGTCAG AAAGGAACAG TGGGAACTCT TCTAATGGAA 
AACCCCGTTG GTCAGAATGG ATTAACATAC CACGGTCTTC TACATGAAAC AGCAAAAGTT 
TTTGGGCTCA GAAGCAGGAG GCTAAAATTG TTCCTGGATG AAGCAGAGAC TCAGGAGATC 
ACAAAGGACA TCTCCATGAA GAACCTGAAC ATGAAGACCG TATATGTCAG TGTTATACCC 
ACCACAGCTG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_414300.4 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_414300.3 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa14086
Clone ID Related Accession (Same CDS sequence)	XM_414300.4 , XM_414300.5
Accession Version	XM_414300.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_414300.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG 
CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT 
GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT 
AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA 
TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG 
CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG 
ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT 
GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG 
CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT 
TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT 
TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC 
ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT 
GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA 
GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG 
AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC 
TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA 
GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA 
GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC 
TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG 
GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT 
ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG 
AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT 
TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA 
ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG 
GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT 
CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT 
GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC 
AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC 
CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC 
AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG 
AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG 
TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA 
GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT 
CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC 
ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC 
ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA 
GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG 
GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT 
TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT 
CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC 
CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT 
TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA 
AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC 
AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG 
AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG 
CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG 
CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG 
GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA 
CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT 
GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA 
GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT 
ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT 
TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG 
GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC 
TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG 
AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT 
TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA 
ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT 
CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA 
AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT 
CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA 
AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC 
TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_414300.3
CDS	37..3882
Translation	MRAGLHQRGLCCEALRMVQQVPENISFPNEEEKILELWKKLNCFQECLKQSKNRPRFNFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNNQCRGIVMRYSKEWEFNVTRLGRWIDFENDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVSFPLVEDASVSLVAWTTTPWTLPSNLALCVNPELQYVKLRDKATGKIYILMESRLIALYKSDSEYEILDRFPGIVLKGKKYKPLFEYFIQCKDQGAFTVLVDSYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDVSGCFTAEVTDFAGQYVKDADKNIIKLLKEKGRLVHSSSYKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNAQCYWVPDFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGVKVTDLHRESIDHLTIPSRCGKGVLRRVPEVFDCWFESGSMPYAQVHYPFENKKELEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPMAIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDILKDVFLPWYNAYRFLVQNVHILQHKDEGREFLYNENTVKESNNIMDKWILSFTQSLIQFFKAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLFSMCRLMAPYTPFITELMYQNLKTLIDPASVQEKNTESIHYLMLPPVREDLIDRKIESAVSCLQSVIELGRVIRDRKTIPVKYPLKEVVVIHQDPEALENIRSLEKYILEELNVRQVTLSTNKDKYGVRLRAEPDHMVLGKRLKGAFKPVMAAIKELKSEQLEEFQETGTIVVEGHELHGEDLRLMYTFDQVAGGSAQFEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYRSHPEGDYLDTVIKEHTDFIFATIKAALKPYPVPTSKEVLIQETTQLKGSELEITLVRGGLCERVGPACSYVNLKVCVNGTEQDGVLLLENPKGDNTLNLTGLVDAVSCIFGLKNSKLTVFNGKTELINKTDLLSLSGKTLHVTTGSAPALINSPDALLCQYINLQLVNAKPQECQKGTVGTLLMENPVGQNGLTYHGLLHETAKVFGLRSRRLKLFLDEAETQEITKDISMKNLNMKTVYVSVIPTTAEC

Target ORF information:

RefSeq Version	XM_414300.4
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_414300.4

ORF Insert Sequence:

ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG 
CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT 
GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT 
AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA 
TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG 
CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG 
ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT 
GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG 
CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT 
TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT 
TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC 
ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT 
GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA 
GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG 
AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC 
TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA 
GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA 
GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC 
TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG 
GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT 
ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG 
AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT 
TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA 
ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG 
GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT 
CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT 
GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC 
AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC 
CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC 
AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG 
AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG 
TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA 
GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT 
CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC 
ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC 
ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA 
GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG 
GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT 
TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT 
CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC 
CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT 
TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA 
AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC 
AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG 
AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG 
CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG 
CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG 
GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA 
CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT 
GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA 
GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT 
ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT 
TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG 
GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC 
TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG 
AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT 
TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA 
ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT 
CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA 
AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT 
CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA 
AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC 
TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS ( XM_414300.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_414300.3 Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

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CloneID	OGa14086
Clone ID Related Accession (Same CDS sequence)	XM_414300.4 , XM_414300.5
Accession Version	XM_414300.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	isoleucine--tRNA ligase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_414300.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG 
CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT 
GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT 
AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA 
TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG 
CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG 
ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT 
GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG 
CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT 
TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT 
TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC 
ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT 
GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA 
GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG 
AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC 
TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA 
GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA 
GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC 
TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG 
GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT 
ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG 
AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT 
TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA 
ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG 
GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT 
CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT 
GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC 
AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC 
CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC 
AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG 
AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG 
TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA 
GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT 
CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC 
ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC 
ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA 
GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG 
GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT 
TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT 
CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC 
CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT 
TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA 
AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC 
AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG 
AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG 
CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG 
CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG 
GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA 
CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT 
GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA 
GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT 
ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT 
TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG 
GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC 
TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG 
AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT 
TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA 
ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT 
CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA 
AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT 
CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA 
AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC 
TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_414300.3
CDS	29..3874
Translation	MRAGLHQRGLCCEALRMVQQVPENISFPNEEEKILELWKKLNCFQECLKQSKNRPRFNFYDGPPFATGLPHYGHILAGTIKDIVTRFAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIKGPEDVAKMGIAEYNNQCRGIVMRYSKEWEFNVTRLGRWIDFENDYKTLYPEFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVIVSFPLVEDASVSLVAWTTTPWTLPSNLALCVNPELQYVKLRDKATGKIYILMESRLIALYKSDSEYEILDRFPGIVLKGKKYKPLFEYFIQCKDQGAFTVLVDSYVKEEEGTGVVHQAPYFGADDYRVCMDFNIIQKDSVPVCPVDVSGCFTAEVTDFAGQYVKDADKNIIKLLKEKGRLVHSSSYKHSYPFCWRSDTPLIYKAVPSWFVRVEHMVEKLLENNAQCYWVPDFVREKRFGNWLKDARDWAISRNRYWGTPIPLWVSDDFEEVVCIGSMAELEELSGVKVTDLHRESIDHLTIPSRCGKGVLRRVPEVFDCWFESGSMPYAQVHYPFENKKELEDAFPADFIAEGIDQTRGWFYTLLVLSTALFGRPPFKNVIVNGLVLASDGQKMSKRKKNYPDPMAIVNSYGADALRLYLINSPVVRAENLRFKEEGVRDILKDVFLPWYNAYRFLVQNVHILQHKDEGREFLYNENTVKESNNIMDKWILSFTQSLIQFFKAEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGTEDCIMALETLFSVLFSMCRLMAPYTPFITELMYQNLKTLIDPASVQEKNTESIHYLMLPPVREDLIDRKIESAVSCLQSVIELGRVIRDRKTIPVKYPLKEVVVIHQDPEALENIRSLEKYILEELNVRQVTLSTNKDKYGVRLRAEPDHMVLGKRLKGAFKPVMAAIKELKSEQLEEFQETGTIVVEGHELHGEDLRLMYTFDQVAGGSAQFEAHSDAQVLVLLDVTPDQSMVDEGVAREVINRIQKLRKKRNLVPTDEITVYYRSHPEGDYLDTVIKEHTDFIFATIKAALKPYPVPTSKEVLIQETTQLKGSELEITLVRGGLCERVGPACSYVNLKVCVNGTEQDGVLLLENPKGDNTLNLTGLVDAVSCIFGLKNSKLTVFNGKTELINKTDLLSLSGKTLHVTTGSAPALINSPDALLCQYINLQLVNAKPQECQKGTVGTLLMENPVGQNGLTYHGLLHETAKVFGLRSRRLKLFLDEAETQEITKDISMKNLNMKTVYVSVIPTTAEC

Target ORF information:

RefSeq Version	XM_414300.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_414300.5

ORF Insert Sequence:

ATGCGCGCGG GGTTGCATCA GCGCGGGCTG TGCTGTGAGG CGCTGAGAAT GGTTCAACAA 
GTTCCAGAAA ACATAAGTTT TCCAAATGAG GAAGAGAAGA TACTGGAGCT CTGGAAGAAG 
CTTAATTGTT TCCAGGAATG CCTAAAGCAG TCAAAGAATA GACCTAGGTT TAACTTCTAT 
GATGGCCCTC CATTTGCTAC CGGGCTTCCG CACTATGGCC ATATTCTTGC AGGAACCATT 
AAAGACATAG TTACCAGATT TGCTCACCAG AGCGGTTTTC ATGTTGACAG AAGATTTGGA 
TGGGATTGCC ATGGCCTACC TGTGGAATAT GAGATTGACA AGACCTTAGG CATCAAAGGG 
CCAGAGGATG TGGCAAAGAT GGGGATTGCA GAATACAACA ACCAGTGCAG AGGAATTGTG 
ATGAGATACT CAAAGGAATG GGAGTTCAAC GTTACCAGAT TAGGACGCTG GATTGATTTT 
GAAAATGACT ATAAAACACT TTATCCTGAA TTCATGGAGT CTGTATGGTG GGTTTTCAAG 
CAGCTTTATG ACAAGGGACT GGTATACAGA GGTGTTAAAG TCATGCCTTT TTCTACTGCT 
TGCAATACCC CATTGTCAAA TTTTGAGTCC CATCAGAATT ACAAGGATGT TCAAGACCCT 
TCGGTGATTG TGAGTTTCCC ACTGGTTGAA GATGCAAGTG TCTCTCTTGT TGCTTGGACC 
ACTACACCTT GGACTTTACC TAGCAATCTG GCCCTTTGTG TTAACCCAGA ACTACAATAT 
GTGAAATTGA GAGATAAGGC TACAGGAAAG ATTTACATCT TGATGGAGTC CAGGCTGATA 
GCACTATACA AATCTGACAG TGAATATGAG ATACTGGACA GGTTTCCTGG CATTGTTCTG 
AAAGGCAAGA AGTATAAACC ACTCTTTGAG TATTTTATTC AGTGCAAAGA TCAAGGTGCC 
TTTACTGTGC TAGTAGACAG TTATGTGAAG GAGGAAGAAG GCACAGGTGT TGTTCATCAA 
GCCCCCTATT TTGGTGCTGA CGATTACAGA GTCTGTATGG ATTTTAATAT TATTCAGAAA 
GATTCTGTCC CAGTTTGTCC TGTTGATGTA TCAGGCTGCT TCACAGCTGA AGTGACCGAC 
TTTGCTGGGC AGTATGTGAA GGATGCAGAT AAGAACATCA TTAAATTACT GAAGGAGAAG 
GGCAGACTGG TTCACAGCAG TAGTTACAAA CATAGCTATC CGTTCTGCTG GAGGTCAGAT 
ACTCCGTTGA TATACAAAGC TGTGCCAAGC TGGTTTGTAA GAGTAGAGCA TATGGTGGAG 
AAGCTACTGG AGAATAATGC ACAGTGCTAC TGGGTTCCGG ATTTTGTGAG GGAGAAGCGT 
TTTGGGAACT GGCTAAAAGA TGCACGAGAT TGGGCTATAT CTCGAAACCG ATACTGGGGA 
ACACCCATCC CACTGTGGGT CAGTGATGAT TTTGAAGAGG TAGTTTGTAT TGGTTCAATG 
GCGGAACTGG AAGAGCTGTC AGGAGTGAAA GTCACTGATC TCCATCGGGA AAGTATTGAT 
CATCTAACCA TCCCTTCACG CTGCGGCAAA GGCGTTTTGC GCCGGGTCCC TGAAGTTTTT 
GATTGCTGGT TTGAGAGTGG AAGCATGCCT TATGCACAAG TGCATTATCC GTTTGAAAAC 
AAAAAAGAAC TTGAAGATGC TTTTCCAGCT GACTTCATTG CTGAAGGCAT TGACCAAACC 
CGAGGATGGT TCTACACTTT GCTGGTGCTG TCCACTGCTC TTTTTGGAAG ACCTCCTTTC 
AAGAATGTAA TTGTAAATGG CCTAGTTCTT GCCAGTGATG GACAAAAAAT GAGCAAAAGG 
AAGAAGAATT ATCCTGATCC AATGGCTATT GTAAATAGTT ATGGAGCTGA TGCACTAAGG 
TTATACCTTA TCAATTCTCC AGTGGTAAGA GCGGAAAACT TGAGATTCAA GGAGGAAGGA 
GTAAGGGACA TACTGAAGGA TGTCTTCCTG CCCTGGTACA ATGCATATCG CTTTCTAGTT 
CAGAACGTTC ACATCCTGCA ACACAAGGAT GAGGGAAGAG AATTCCTTTA CAATGAGAAC 
ACAGTTAAGG AGAGCAATAA CATCATGGAT AAATGGATTC TTTCTTTCAC CCAATCGCTC 
ATTCAGTTCT TCAAAGCTGA AATGGCAGCT TACAGGCTGT ACACGGTAGT CCCTCGGTTA 
GTGAAGTTTG TAGACATTCT GACCAACTGG TATGTGAGAA TGAATCGCAG AAGACTGAAG 
GGCGAGAATG GGACTGAGGA CTGCATTATG GCCCTGGAAA CCTTGTTCAG TGTTTTGTTT 
TCCATGTGCA GACTTATGGC TCCGTATACT CCATTTATCA CTGAACTGAT GTACCAGAAT 
CTGAAGACGC TTATTGATCC AGCCTCAGTT CAGGAGAAGA ATACTGAAAG CATTCACTAC 
CTCATGCTTC CCCCAGTGAG GGAGGATCTG ATCGACAGAA AAATTGAAAG TGCTGTTTCT 
TGTTTGCAGT CTGTTATTGA GCTTGGACGA GTAATCAGAG ACAGAAAAAC CATTCCAGTA 
AAGTATCCTT TGAAAGAAGT AGTTGTTATC CATCAAGACC CAGAGGCTCT GGAAAACATC 
AGGTCTCTAG AGAAGTACAT ACTTGAGGAG CTGAACGTAC GCCAGGTGAC GTTATCCACG 
AATAAAGATA AATATGGAGT CAGGCTGAGA GCAGAACCGG ATCACATGGT GCTTGGGAAG 
CGTTTGAAAG GTGCATTTAA GCCTGTCATG GCTGCAATCA AAGAGCTGAA GAGTGAGCAG 
CTGGAGGAAT TCCAAGAGAC AGGCACCATT GTTGTAGAAG GACACGAGCT CCATGGGGAG 
GATCTTCGTC TCATGTATAC TTTTGATCAG GTTGCAGGAG GATCTGCTCA GTTTGAGGCA 
CATTCTGATG CTCAGGTTTT AGTTCTGCTG GATGTTACCC CAGACCAGTC TATGGTGGAT 
GAAGGAGTGG CCCGGGAAGT GATCAACCGC ATTCAGAAGC TTCGCAAAAA GCGAAATCTA 
GTTCCCACAG ATGAGATTAC AGTGTACTAC AGATCACATC CAGAAGGTGA TTACTTGGAT 
ACTGTCATTA AGGAGCATAC AGATTTCATT TTTGCAACAA TAAAGGCTGC CCTGAAGCCT 
TACCCGGTTC CTACCTCAAA AGAAGTTCTC ATCCAGGAGA CCACTCAGCT GAAGGGATCG 
GAGCTAGAAA TTACACTTGT CAGAGGTGGT TTGTGTGAGC GTGTTGGTCC AGCCTGTTCC 
TACGTCAACC TCAAAGTCTG TGTTAATGGG ACAGAACAAG ATGGTGTGTT GCTGCTGGAG 
AATCCAAAAG GAGATAATAC GTTGAACCTC ACTGGACTTG TCGATGCAGT CTCCTGCATT 
TTTGGACTTA AAAATTCAAA ACTGACAGTT TTTAATGGAA AAACAGAACT GATAAACAAA 
ACTGACTTGC TTAGTCTCAG TGGAAAGACT CTGCATGTGA CAACGGGGTC AGCACCTGCT 
CTCATCAACT CTCCTGATGC TCTTCTCTGC CAGTATATCA ACTTGCAGTT AGTAAATGCA 
AAACCACAAG AGTGTCAGAA AGGAACAGTG GGAACTCTTC TAATGGAAAA CCCCGTTGGT 
CAGAATGGAT TAACATACCA CGGTCTTCTA CATGAAACAG CAAAAGTTTT TGGGCTCAGA 
AGCAGGAGGC TAAAATTGTT CCTGGATGAA GCAGAGACTC AGGAGATCAC AAAGGACATC 
TCCATGAAGA ACCTGAACAT GAAGACCGTA TATGTCAGTG TTATACCCAC CACAGCTGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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IARS cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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