pyr-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	pyr-1
Entrez Gene ID	174385
Full Name	Glutamine-dependent carbamoyl-phosphate synthase
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_063437.4	NP_495838.2	Glutamine-dependent carbamoyl-phosphate synthase
NM_063437.5	NP_495838.2	Glutamine-dependent carbamoyl-phosphate synthase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pyr-1 functions upstream of proteoglycan synthesizing enzymes by providing precursors of UDP-sugars essential for heparan sulfate proteoglycan synthesis.
Title: C. elegans pharyngeal morphogenesis requires both de novo synthesis of pyrimidines and synthesis of heparan sulfate proteoglycans.

The following pyr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pyr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa337543	NM_063437.5 Latest version!	Caenorhabditis elegans Glutamine-dependent carbamoyl-phosphate synthase (pyr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OCa337543	NM_063437.4	Caenorhabditis elegans Glutamine-dependent carbamoyl-phosphate synthase (pyr-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pyr-1 ( NM_063437.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495838.2 Caenorhabditis elegans Glutamine-dependent carbamoyl-phosphate synthase (pyr-1), partial mRNA.

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CloneID	OCa337543
Clone ID Related Accession (Same CDS sequence)	NM_063437.4 , NM_063437.5
Accession Version	NM_063437.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Glutamine-dependent carbamoyl-phosphate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_063437.3. COMPLETENESS: incomplete on both ends.

ATGAGAGCGA CTTTGCATCT CGAGGACGGG TCCACTTTTG TGGGCTCCAT TTATGGAGCA 
ACCAAAAGTG TAGTTGGAGA AATTGTTTTT CAAACCGGAA TGGTTGGTTA TGTGGAATCT 
CTTACCGATC CATCATACGC CAAGCAGCTT CTCACACTTA CATATCCACT TATTGGAAAT 
TACGGAGTTC CATCTGCAGA GATTCTGGAT CAGTTCAAGC TACCAGCTGA ATTTGAGTCT 
GACAGGATCT GGCCAGCAGC TCTCATTGTT GAGAAAATTT GTGTCGATGG AGAACACAGT 
CATTGGCAAG CAGTTCAGTC GTTATCAGAA TGGCTCAGAA AAGCTGATGT TCCATGTCTT 
TCCGGAATTG ATGTTCGCCA ATTGGTGAAG AAAATCAGAG AGACTGGAAC AATGAAGGCG 
AAGCTTGTGA TCGAATCAGA CAATGCACAA AACTTCGACT ACGTTGACGT CAATGCTGAA 
AATTTGGTTG ACTTCGTGTC GAGAAAAGAG CCAGTTGTCT ATGGATCCGG TGATCAAACC 
ATTCTCGCAG TTGATTGTGG TCTCAAGAAT AATCAAATTA GATGTCTCGC AAAACGAGGA 
TTTCGTGTCA AAGTGGTTCC TTGGAATCAT CCAATTGATA CTGAAAGTGA TTATGATGGA 
CTTTTCCTTT CCAACGGACC AGGAGATCCA GAAATTTGTG CACCACTGGT CGATCGATTA 
GCCAAAGTTA TTGCAAGAGG AGACAAACCA ATTTTTGGAA TTTGTCTCGG ACACCAAATT 
TTGTCTAGAG CTATCGGAGC GAAAACCTAC AAATTGAAAT ACGGTAACCG TGGTCACAAT 
CAGCCATGCA CTCATTATGC TACTGGCCGT TGCTACATCA CTTCTCAAAA TCATGGATAC 
GCAGTTGATC CAGATTCACT TCCAGCTGAT TGGAAGGCAC TTTTCACGAA TGAGAATGAC 
AAGACCAACG AAGGAATTGT GCACTCGTCT AAACCATTTT TCAGTGTTCA ATTCCATCCA 
GAACACACTG CCGGGCCAAC TGATTGCGAG TTTTTGTTCG ATGTTTTTGC TGATTCTGTG 
AGACAAGCAA AGAGTGGCAC ATTCATGAAT GTCGATCAAG AGCTCACTCG TCTTATGACT 
TTCACTCCCA TCTATCATGC CAAAGAACAG AGAAAAGTTC TCGTTCTCGG ATCAGGCGGT 
CTCACTATTG GTCAAGCTGG AGAGTTTGAT TATTCTGGGG CTCAAGCTCT GAAGGCATTG 
AGAGAAGAAG GAATTCGAAC AGTTCTCATC AACCCAAACA TTGCTACAGT TCAAACCTCC 
AAAGGCTTTG CTGATTTCAC TTACTTCCTT CCAATCACTA AAGAATACGT TACCGATGTG 
ATCAAGAAGG AGCGACCAAC TGGAATCTTG TGTACCTTTG GAGGTCAAAC TGCTCTCAAC 
TGCGCCATCG ACTTGTATAA AGATGGAATT TTTGAGCAAT ACGATGTGCA GGTTCTTGGA 
ACTCAAATCA ACACAATTAT GAAAACAGAA GATCGCGATT TGTTCAATCA AGAAATTTCG 
GCTATTGGAG AAAAGGTTGC ACCAAGTAAA GCAGCGACGA CCATGGAAGG TGCCATTGAA 
GCAGCTGAAG AGTTGGGTTA TCCAGTTCTT GTCAGAGCCG CATATGCTCT TGGTGGACTT 
GGATCAGGAT TCGCTGACAA CCGGGAAGAG CTCATTGCAA TTGCTCAACA AGCTTTAGCA 
CACTCAAATC AGGTTCTTGT TGATAAGAGT TTGAAAGGAT GGAAGGAAGT GGAATATGAA 
GTTGTTCGGG ACGCTTACGA TAATTGTATT ACCGTTTGCA ACATGGAAAA TGTGGATCCA 
CTTGGAATTC ATACTGGTGA AAGTGTTGTT GTTGCACCAT CTCAAACTCT TTCTGACAGA 
GAATACAATG CTCTTAGAAC ATGTGCAATC AAAGTTATTC GGCACTTGGG AATTATTGGA 
GAGTGTAATA TTCAATACGC TCTGGATCCG TATTCCTTGA CTTATTACAT TATCGAAGTC 
AATGCACGAC TTTCTCGTTC TTCTGCTCTT GCCTCAAAAG CCACCGGATA CCCATTGGCA 
TATGTTGCTG CAAAGTTGGC ATTGGGACAA CATCTGCCAG TCATCCGAAA TAGTGTCACT 
GGAACCACAA CTGCATGCTT TGAGCCAAGT TTGGACTATT GTGTTGTGAA GATTCCTCGT 
TGGGATCTTG GAAAATTCGC TCGTGTCAGC ACACAAATTG GAAGCTCTAT GAAATCAGTT 
GGAGAAGTCA TGGGAATCGG GAGATGCTTT GAAGAGGCTC TTCAGAAGGC GTTGAGAATG 
GTGTCCGATC ATGCTGACGG TTTCTCTCCA TACACATTCA GTCGTCCTAC AACAGCTGAT 
GATCTTTCAA AGCCTACAGA TAAGAGAATG TTTGCATTGG CACGTGGAAT GTATTATGGA 
GACTTTGATG TTGAAAAAGC TCACGAGCTG ACACGTATTG ATCGTTGGTT CTTGTTCAGA 
ATGCAGAACA TCGTTGATAT TTACCATCGC CTCGAAAAGA CTGACGTCAA TACAGTATCT 
GCTGAACTGC TCCTGGAGGC GAAACAAGCA GGATTCTCCG ACAGACAAAT AGCAAAGAAA 
ATCGGAAGCA ACGAATACAC AGTTCGCGAA GCTCGTTTCG TCAAAGGAAT CACTCCTTGC 
GTGAAGCAAA TTGACACAGT TGCTGGAGAA TGGCCTGCTC AGACAAACTA CCTCTACACA 
ACATTCAATG GAATAGAGAA TGATGTTTCA TTCAATATGA AGAACGCTGT GATGGTTCTC 
GGATCAGGAG TATACCGAAT CGGTTCTTCA GTTGAATTTG ATTCTTCTTG CGTTGGATGC 
ATCAGAGAGT TGAAGGCTTT GGGATACTCT ACAATCACCG TCAATTGCAA TCCTGAAACT 
GTTTCTACGG ATTATGATAT TTGTGATCGA TTGTATTTTG AAGAGATTTC TTTTGAAACC 
GTTCTCGACG TTTATCATTT GGAAAAACCA AAGGGAGTGA TTCTGGCATT TGGAGGTCAA 
GCACCGAATA ACATTGCGAT GAGCTTGAGC AGAGCTCAGG TGAAGATCTT TGGAACTTCA 
CCAAACGACA TTGATAACGC AGAAGATAGA TTCAAGTTCT CAAGAAAATT GGAATCTTTG 
AAAATCAGCC AGCCACAATG GAAGAAAAGC GAAAATATGG AAGATGCTAA GAACTTCTGT 
GCTCAAGTTG GATATCCATG CTTGATCCGT CCTTCATATG TTTTATCTGG TGCAGCAATG 
AATGTGGCTC ACAACGCTGA AGACTTGGAA GTCTTTCTCA AGCAAGCTGC TGTGGTCGCC 
AAGGAGCATC CAGTTGTTGT GAGCAAATTT ATCAATGAAG CGAAGGAATT GGATGTAGAT 
GCAGTTGCCC TTGATGGAAA ACTCGTTGTA ATGGCTGTCA GTGAACATAT TGAAAATGCA 
GGTGTTCATT CGGGAGATGC TACTCTTGTC ACTCCAGCTC AGGATATGAA CAAACTGACA 
TTGGATAGAA TCAAGGATAT CACTTTCAGA ATCGCTGAAG CATTTAATGT CAACGGACCT 
TTCAATATGC AGCTCATTGC TAAAAATAAC GAACTCAAAG TTATTGAATG CAACTTGCGA 
GTATCCAGAT CATTCCCGTT CGTCTCAAAA ACTCTCGATT ACGATTTTGT GGCTTTGGCT 
ACTCGTGCAA TGATGGCTTC AGATTCACCT GCAATTCGAG CTACAATCAA ACCAACAGCC 
ACCTTGCTCA AAGGAAAGGG AAGAGTCGGA GTGAAGGTTC CTCAATTCTC TTTCTCAAGA 
CTCGCTGGTG CCGATGTGAT GCTTGGTGTC GAGATGGCTT CAACTGGAGA AGTTGCTTGC 
TTCGGAACTT CAAGATGCGA TGCCTACCTC AAAGCTTTAC TCTCCACTGG TTTCGTTGTT 
CCAAAACAAA ATATTTTCAT TTCCATTGGA GGATATCACG CGAAGGCTGA GATGCTCAAG 
TCTGTAGAAG CATTGCTGAA GTTGGGATAC GAATTGTATG GATCCAAAGG AACTGCCGAC 
TATTTCCAAT CGAATAAGAT CAATGTGAAG CCAGTCGATT GGCCTTTTGA AGAAGGATCG 
TCCGATGAGA AAACTGCAAG TGGAACAAGA TCTGTAGTTG AATTCCTCGA GAACAAAGAA 
TTCCACTTGG TCATCAACCT ACCGATTCGT GGATCAGGAG CATACCGAGT CTCTGCCTTC 
CGCACTCATG GTTACAAGAC GAGAAGGATG GCAATTGACA ATGGAATTCC CTTGATAACT 
GATATTAAGT GTGCTAAGAC CTTCATTCAA GCCCTTGAAA TGGTTGGAAA ACGTCCAACT 
ATGAATTCAT TGGTCGATTG TGTGACTTCC AAGAGTTTGA AGCGTTTGCC AGGAATGGTT 
GATATTCATG TACATGTTAG AGAACCAGGA GCAACACACA AAGAAGATTG GGCAACTTGT 
TCAAAGGCTG CACTAGCTGG AGGTGTTACT ACAATTTTGG CAATGCCAAA CACTTCTCCT 
GTTCTTGTGG ATACTGATTC CTTCTATCAA ACGGAACAGT TGGCTTCTGC AAAATCGGTC 
GTCGACTACG CCCTCTACAT CGGAGCTACT CCAAACAATT CGAAGTTTGC AGCGGAGTTT 
GCTGACAAAG CCGCTGGCTT GAAGATGTAT CTCAATGAGA CATTCAGTAC TCTGAAGATG 
GATAATATTT CAGACTGGGC TAAGCACCTG AGTGCCTTCC CTGCTAATCG TCCGATCGTC 
TGTCACGCCG AGAAGCAAAC ACTTGCCGCC ATTCTTTGTA TGGCTCAGAT GGCGAATAGA 
GCAGTTCACA TTGCCCACGT CGCTACGGCT GATGAGATTA ATCTCGTCAA GGAAGCTAAA 
CAAAGAGGAT GGAATGTTAC CTGTGAAGTC TGCCCACATC ATCTTTTCCT CATTGAAGAA 
GATCTTCCTG ATGGAATTCG TGAAGTTCGT CCACGTCTCG TAAAACCAGA AGATCGTCAG 
GCTCTTTGGG ACAACATGGA ATACATTGAT TGTTTTGCAA CTGATCATGC ACCTCATACG 
TGGGCTGAGA AGACTGGAAA AGATGGGAAA ATTCCACCAG GCTTCCCTGG AGTTGAGTAC 
ATGCTCCCAC TTCTTCTTAC AGCTGTGCAT GATGGTAAAT TGACCATGAA GGAACTCACT 
GATCGTATGT CCACCAATCC TCGTCGAATT TTCAACTTGC CTCCACAAGA TGATACCTAC 
ATTGAGGTTG ATTTGAACGA AGAATGGACA ATTCCAGAGA ATGGAGGACA GAGCAAAGCT 
GGATGGACTC CATTTGCTGG TAGAAAAGTG TTTGGAAAAG TGCACAATGT GATCATCAGA 
GGAGAAGAAG CTGTTATTGA TGGAAGAATC GTTGCAATTC CAGGATTCGG AAAGAATGTC 
CGTCTTTATC CACACTCTGG AACTGCTCAT CGTGGAGATT CAGACTTCGA TCAAATCTTG 
GAACCAATTC CTCAACAAAT GATTGAAAGT TCTTCAGATG AACAAAGTCC ACTTCATACT 
CCACCTAGAG CTCATACGCC GATTGCATTC CCTGGAGAGC TTCTTGCCAA GAATTGTATC 
AGTGTGAAGC ATCTTGATAA GGGCCAGATC AATCGTATTT TCGAGTTGGC GGACCGATAC 
AAGCACGATG TCGAAAAGGG CCACCCATTG ACACATATTC TGAATGGAAA AGTATTGGTC 
AATCTATTCT ACGAAGTGTC AACAAGAACA TCTTGCTCGT TCTCTGCAGC CATGCAACGT 
CTTGGAGGAT CTGTCATCTC TGTTGATAGT CAATCATCTT CAGTTCAAAA GGGAGAAACT 
TTGGAAGACA CAGTGCAGGT TTTGGGAAGT TACGGAGATA TTCTGGTTCT GAGAAGTAAT 
GAGAATGGAG CTGCTGATAG AGCTGCTCGA GTTTGCGATC AACCGGTCAT CAATGGAGGT 
GATGGAACTG GTGAACATCC AACTCAAGCT CTTCTCGATG TGTACACTAT TCGACAAGAA 
ATGGGAACAG TCAATGGACT TACCATCGCT TTGGTTGGAG ATTTGAAGAA TGGAAGAACG 
GTTCACTCAT TGGCTAAACT TTTATGTTTG TATAAGGACA TCACCCTTCA CTACGTTGCT 
CCGAGTACCG AACTTGAAAT GCCACAGGAA GTCTTGGACT ACGTCTCGTC GAAGTCGAAT 
TTTGTTCAGA AGAAGTTCAC CAGTCTGGCT GAAGGAATCA ATCATGTCGA CGTTGTCTAC 
GTGACAAGAA TTCAAAAAGA ACGATTCTCA TCTCCAGATG AATACAATAA GGTGAAAGGA 
AGCTACGTAA TCAATGCGAA ACTTCTCAAT GAGGCCGCCA GAGATGTCGA GGAACCATCG 
AGTCTGTTGG TTCCAGCTCG TTCTCTTCCA ATCGTCATGC ATCCATTGCC TCGTGTGGAT 
GAGATTGCTG TTGAACTGGA TCATGATGAA AGAGCCGCCT ACTTCAGACA AGCAAAGAAT 
GGGGTCTTCG TTCGTATGTC TATTCTATCC CTTCTTCTTG GAAGAGGGCA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495838.2
CDS	1..6597
Translation	MRATLHLEDGSTFVGSIYGATKSVVGEIVFQTGMVGYVESLTDPSYAKQLLTLTYPLIGNYGVPSAEILDQFKLPAEFESDRIWPAALIVEKICVDGEHSHWQAVQSLSEWLRKADVPCLSGIDVRQLVKKIRETGTMKAKLVIESDNAQNFDYVDVNAENLVDFVSRKEPVVYGSGDQTILAVDCGLKNNQIRCLAKRGFRVKVVPWNHPIDTESDYDGLFLSNGPGDPEICAPLVDRLAKVIARGDKPIFGICLGHQILSRAIGAKTYKLKYGNRGHNQPCTHYATGRCYITSQNHGYAVDPDSLPADWKALFTNENDKTNEGIVHSSKPFFSVQFHPEHTAGPTDCEFLFDVFADSVRQAKSGTFMNVDQELTRLMTFTPIYHAKEQRKVLVLGSGGLTIGQAGEFDYSGAQALKALREEGIRTVLINPNIATVQTSKGFADFTYFLPITKEYVTDVIKKERPTGILCTFGGQTALNCAIDLYKDGIFEQYDVQVLGTQINTIMKTEDRDLFNQEISAIGEKVAPSKAATTMEGAIEAAEELGYPVLVRAAYALGGLGSGFADNREELIAIAQQALAHSNQVLVDKSLKGWKEVEYEVVRDAYDNCITVCNMENVDPLGIHTGESVVVAPSQTLSDREYNALRTCAIKVIRHLGIIGECNIQYALDPYSLTYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGQHLPVIRNSVTGTTTACFEPSLDYCVVKIPRWDLGKFARVSTQIGSSMKSVGEVMGIGRCFEEALQKALRMVSDHADGFSPYTFSRPTTADDLSKPTDKRMFALARGMYYGDFDVEKAHELTRIDRWFLFRMQNIVDIYHRLEKTDVNTVSAELLLEAKQAGFSDRQIAKKIGSNEYTVREARFVKGITPCVKQIDTVAGEWPAQTNYLYTTFNGIENDVSFNMKNAVMVLGSGVYRIGSSVEFDSSCVGCIRELKALGYSTITVNCNPETVSTDYDICDRLYFEEISFETVLDVYHLEKPKGVILAFGGQAPNNIAMSLSRAQVKIFGTSPNDIDNAEDRFKFSRKLESLKISQPQWKKSENMEDAKNFCAQVGYPCLIRPSYVLSGAAMNVAHNAEDLEVFLKQAAVVAKEHPVVVSKFINEAKELDVDAVALDGKLVVMAVSEHIENAGVHSGDATLVTPAQDMNKLTLDRIKDITFRIAEAFNVNGPFNMQLIAKNNELKVIECNLRVSRSFPFVSKTLDYDFVALATRAMMASDSPAIRATIKPTATLLKGKGRVGVKVPQFSFSRLAGADVMLGVEMASTGEVACFGTSRCDAYLKALLSTGFVVPKQNIFISIGGYHAKAEMLKSVEALLKLGYELYGSKGTADYFQSNKINVKPVDWPFEEGSSDEKTASGTRSVVEFLENKEFHLVINLPIRGSGAYRVSAFRTHGYKTRRMAIDNGIPLITDIKCAKTFIQALEMVGKRPTMNSLVDCVTSKSLKRLPGMVDIHVHVREPGATHKEDWATCSKAALAGGVTTILAMPNTSPVLVDTDSFYQTEQLASAKSVVDYALYIGATPNNSKFAAEFADKAAGLKMYLNETFSTLKMDNISDWAKHLSAFPANRPIVCHAEKQTLAAILCMAQMANRAVHIAHVATADEINLVKEAKQRGWNVTCEVCPHHLFLIEEDLPDGIREVRPRLVKPEDRQALWDNMEYIDCFATDHAPHTWAEKTGKDGKIPPGFPGVEYMLPLLLTAVHDGKLTMKELTDRMSTNPRRIFNLPPQDDTYIEVDLNEEWTIPENGGQSKAGWTPFAGRKVFGKVHNVIIRGEEAVIDGRIVAIPGFGKNVRLYPHSGTAHRGDSDFDQILEPIPQQMIESSSDEQSPLHTPPRAHTPIAFPGELLAKNCISVKHLDKGQINRIFELADRYKHDVEKGHPLTHILNGKVLVNLFYEVSTRTSCSFSAAMQRLGGSVISVDSQSSSVQKGETLEDTVQVLGSYGDILVLRSNENGAADRAARVCDQPVINGGDGTGEHPTQALLDVYTIRQEMGTVNGLTIALVGDLKNGRTVHSLAKLLCLYKDITLHYVAPSTELEMPQEVLDYVSSKSNFVQKKFTSLAEGINHVDVVYVTRIQKERFSSPDEYNKVKGSYVINAKLLNEAARDVEEPSSLLVPARSLPIVMHPLPRVDEIAVELDHDERAAYFRQAKNGVFVRMSILSLLLGRGHL

Target ORF information:

RefSeq Version	NM_063437.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Glutamine-dependent carbamoyl-phosphate synthase (pyr-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063437.4

ORF Insert Sequence:

ATGAGAGCGA CTTTGCATCT CGAGGACGGG TCCACTTTTG TGGGCTCCAT TTATGGAGCA 
ACCAAAAGTG TAGTTGGAGA AATTGTTTTT CAAACCGGAA TGGTTGGTTA TGTGGAATCT 
CTTACCGATC CATCATACGC CAAGCAGCTT CTCACACTTA CATATCCACT TATTGGAAAT 
TACGGAGTTC CATCTGCAGA GATTCTGGAT CAGTTCAAGC TACCAGCTGA ATTTGAGTCT 
GACAGGATCT GGCCAGCAGC TCTCATTGTT GAGAAAATTT GTGTCGATGG AGAACACAGT 
CATTGGCAAG CAGTTCAGTC GTTATCAGAA TGGCTCAGAA AAGCTGATGT TCCATGTCTT 
TCCGGAATTG ATGTTCGCCA ATTGGTGAAG AAAATCAGAG AGACTGGAAC AATGAAGGCG 
AAGCTTGTGA TCGAATCAGA CAATGCACAA AACTTCGACT ACGTTGACGT CAATGCTGAA 
AATTTGGTTG ACTTCGTGTC GAGAAAAGAG CCAGTTGTCT ATGGATCCGG TGATCAAACC 
ATTCTCGCAG TTGATTGTGG TCTCAAGAAT AATCAAATTA GATGTCTCGC AAAACGAGGA 
TTTCGTGTCA AAGTGGTTCC TTGGAATCAT CCAATTGATA CTGAAAGTGA TTATGATGGA 
CTTTTCCTTT CCAACGGACC AGGAGATCCA GAAATTTGTG CACCACTGGT CGATCGATTA 
GCCAAAGTTA TTGCAAGAGG AGACAAACCA ATTTTTGGAA TTTGTCTCGG ACACCAAATT 
TTGTCTAGAG CTATCGGAGC GAAAACCTAC AAATTGAAAT ACGGTAACCG TGGTCACAAT 
CAGCCATGCA CTCATTATGC TACTGGCCGT TGCTACATCA CTTCTCAAAA TCATGGATAC 
GCAGTTGATC CAGATTCACT TCCAGCTGAT TGGAAGGCAC TTTTCACGAA TGAGAATGAC 
AAGACCAACG AAGGAATTGT GCACTCGTCT AAACCATTTT TCAGTGTTCA ATTCCATCCA 
GAACACACTG CCGGGCCAAC TGATTGCGAG TTTTTGTTCG ATGTTTTTGC TGATTCTGTG 
AGACAAGCAA AGAGTGGCAC ATTCATGAAT GTCGATCAAG AGCTCACTCG TCTTATGACT 
TTCACTCCCA TCTATCATGC CAAAGAACAG AGAAAAGTTC TCGTTCTCGG ATCAGGCGGT 
CTCACTATTG GTCAAGCTGG AGAGTTTGAT TATTCTGGGG CTCAAGCTCT GAAGGCATTG 
AGAGAAGAAG GAATTCGAAC AGTTCTCATC AACCCAAACA TTGCTACAGT TCAAACCTCC 
AAAGGCTTTG CTGATTTCAC TTACTTCCTT CCAATCACTA AAGAATACGT TACCGATGTG 
ATCAAGAAGG AGCGACCAAC TGGAATCTTG TGTACCTTTG GAGGTCAAAC TGCTCTCAAC 
TGCGCCATCG ACTTGTATAA AGATGGAATT TTTGAGCAAT ACGATGTGCA GGTTCTTGGA 
ACTCAAATCA ACACAATTAT GAAAACAGAA GATCGCGATT TGTTCAATCA AGAAATTTCG 
GCTATTGGAG AAAAGGTTGC ACCAAGTAAA GCAGCGACGA CCATGGAAGG TGCCATTGAA 
GCAGCTGAAG AGTTGGGTTA TCCAGTTCTT GTCAGAGCCG CATATGCTCT TGGTGGACTT 
GGATCAGGAT TCGCTGACAA CCGGGAAGAG CTCATTGCAA TTGCTCAACA AGCTTTAGCA 
CACTCAAATC AGGTTCTTGT TGATAAGAGT TTGAAAGGAT GGAAGGAAGT GGAATATGAA 
GTTGTTCGGG ACGCTTACGA TAATTGTATT ACCGTTTGCA ACATGGAAAA TGTGGATCCA 
CTTGGAATTC ATACTGGTGA AAGTGTTGTT GTTGCACCAT CTCAAACTCT TTCTGACAGA 
GAATACAATG CTCTTAGAAC ATGTGCAATC AAAGTTATTC GGCACTTGGG AATTATTGGA 
GAGTGTAATA TTCAATACGC TCTGGATCCG TATTCCTTGA CTTATTACAT TATCGAAGTC 
AATGCACGAC TTTCTCGTTC TTCTGCTCTT GCCTCAAAAG CCACCGGATA CCCATTGGCA 
TATGTTGCTG CAAAGTTGGC ATTGGGACAA CATCTGCCAG TCATCCGAAA TAGTGTCACT 
GGAACCACAA CTGCATGCTT TGAGCCAAGT TTGGACTATT GTGTTGTGAA GATTCCTCGT 
TGGGATCTTG GAAAATTCGC TCGTGTCAGC ACACAAATTG GAAGCTCTAT GAAATCAGTT 
GGAGAAGTCA TGGGAATCGG GAGATGCTTT GAAGAGGCTC TTCAGAAGGC GTTGAGAATG 
GTGTCCGATC ATGCTGACGG TTTCTCTCCA TACACATTCA GTCGTCCTAC AACAGCTGAT 
GATCTTTCAA AGCCTACAGA TAAGAGAATG TTTGCATTGG CACGTGGAAT GTATTATGGA 
GACTTTGATG TTGAAAAAGC TCACGAGCTG ACACGTATTG ATCGTTGGTT CTTGTTCAGA 
ATGCAGAACA TCGTTGATAT TTACCATCGC CTCGAAAAGA CTGACGTCAA TACAGTATCT 
GCTGAACTGC TCCTGGAGGC GAAACAAGCA GGATTCTCCG ACAGACAAAT AGCAAAGAAA 
ATCGGAAGCA ACGAATACAC AGTTCGCGAA GCTCGTTTCG TCAAAGGAAT CACTCCTTGC 
GTGAAGCAAA TTGACACAGT TGCTGGAGAA TGGCCTGCTC AGACAAACTA CCTCTACACA 
ACATTCAATG GAATAGAGAA TGATGTTTCA TTCAATATGA AGAACGCTGT GATGGTTCTC 
GGATCAGGAG TATACCGAAT CGGTTCTTCA GTTGAATTTG ATTCTTCTTG CGTTGGATGC 
ATCAGAGAGT TGAAGGCTTT GGGATACTCT ACAATCACCG TCAATTGCAA TCCTGAAACT 
GTTTCTACGG ATTATGATAT TTGTGATCGA TTGTATTTTG AAGAGATTTC TTTTGAAACC 
GTTCTCGACG TTTATCATTT GGAAAAACCA AAGGGAGTGA TTCTGGCATT TGGAGGTCAA 
GCACCGAATA ACATTGCGAT GAGCTTGAGC AGAGCTCAGG TGAAGATCTT TGGAACTTCA 
CCAAACGACA TTGATAACGC AGAAGATAGA TTCAAGTTCT CAAGAAAATT GGAATCTTTG 
AAAATCAGCC AGCCACAATG GAAGAAAAGC GAAAATATGG AAGATGCTAA GAACTTCTGT 
GCTCAAGTTG GATATCCATG CTTGATCCGT CCTTCATATG TTTTATCTGG TGCAGCAATG 
AATGTGGCTC ACAACGCTGA AGACTTGGAA GTCTTTCTCA AGCAAGCTGC TGTGGTCGCC 
AAGGAGCATC CAGTTGTTGT GAGCAAATTT ATCAATGAAG CGAAGGAATT GGATGTAGAT 
GCAGTTGCCC TTGATGGAAA ACTCGTTGTA ATGGCTGTCA GTGAACATAT TGAAAATGCA 
GGTGTTCATT CGGGAGATGC TACTCTTGTC ACTCCAGCTC AGGATATGAA CAAACTGACA 
TTGGATAGAA TCAAGGATAT CACTTTCAGA ATCGCTGAAG CATTTAATGT CAACGGACCT 
TTCAATATGC AGCTCATTGC TAAAAATAAC GAACTCAAAG TTATTGAATG CAACTTGCGA 
GTATCCAGAT CATTCCCGTT CGTCTCAAAA ACTCTCGATT ACGATTTTGT GGCTTTGGCT 
ACTCGTGCAA TGATGGCTTC AGATTCACCT GCAATTCGAG CTACAATCAA ACCAACAGCC 
ACCTTGCTCA AAGGAAAGGG AAGAGTCGGA GTGAAGGTTC CTCAATTCTC TTTCTCAAGA 
CTCGCTGGTG CCGATGTGAT GCTTGGTGTC GAGATGGCTT CAACTGGAGA AGTTGCTTGC 
TTCGGAACTT CAAGATGCGA TGCCTACCTC AAAGCTTTAC TCTCCACTGG TTTCGTTGTT 
CCAAAACAAA ATATTTTCAT TTCCATTGGA GGATATCACG CGAAGGCTGA GATGCTCAAG 
TCTGTAGAAG CATTGCTGAA GTTGGGATAC GAATTGTATG GATCCAAAGG AACTGCCGAC 
TATTTCCAAT CGAATAAGAT CAATGTGAAG CCAGTCGATT GGCCTTTTGA AGAAGGATCG 
TCCGATGAGA AAACTGCAAG TGGAACAAGA TCTGTAGTTG AATTCCTCGA GAACAAAGAA 
TTCCACTTGG TCATCAACCT ACCGATTCGT GGATCAGGAG CATACCGAGT CTCTGCCTTC 
CGCACTCATG GTTACAAGAC GAGAAGGATG GCAATTGACA ATGGAATTCC CTTGATAACT 
GATATTAAGT GTGCTAAGAC CTTCATTCAA GCCCTTGAAA TGGTTGGAAA ACGTCCAACT 
ATGAATTCAT TGGTCGATTG TGTGACTTCC AAGAGTTTGA AGCGTTTGCC AGGAATGGTT 
GATATTCATG TACATGTTAG AGAACCAGGA GCAACACACA AAGAAGATTG GGCAACTTGT 
TCAAAGGCTG CACTAGCTGG AGGTGTTACT ACAATTTTGG CAATGCCAAA CACTTCTCCT 
GTTCTTGTGG ATACTGATTC CTTCTATCAA ACGGAACAGT TGGCTTCTGC AAAATCGGTC 
GTCGACTACG CCCTCTACAT CGGAGCTACT CCAAACAATT CGAAGTTTGC AGCGGAGTTT 
GCTGACAAAG CCGCTGGCTT GAAGATGTAT CTCAATGAGA CATTCAGTAC TCTGAAGATG 
GATAATATTT CAGACTGGGC TAAGCACCTG AGTGCCTTCC CTGCTAATCG TCCGATCGTC 
TGTCACGCCG AGAAGCAAAC ACTTGCCGCC ATTCTTTGTA TGGCTCAGAT GGCGAATAGA 
GCAGTTCACA TTGCCCACGT CGCTACGGCT GATGAGATTA ATCTCGTCAA GGAAGCTAAA 
CAAAGAGGAT GGAATGTTAC CTGTGAAGTC TGCCCACATC ATCTTTTCCT CATTGAAGAA 
GATCTTCCTG ATGGAATTCG TGAAGTTCGT CCACGTCTCG TAAAACCAGA AGATCGTCAG 
GCTCTTTGGG ACAACATGGA ATACATTGAT TGTTTTGCAA CTGATCATGC ACCTCATACG 
TGGGCTGAGA AGACTGGAAA AGATGGGAAA ATTCCACCAG GCTTCCCTGG AGTTGAGTAC 
ATGCTCCCAC TTCTTCTTAC AGCTGTGCAT GATGGTAAAT TGACCATGAA GGAACTCACT 
GATCGTATGT CCACCAATCC TCGTCGAATT TTCAACTTGC CTCCACAAGA TGATACCTAC 
ATTGAGGTTG ATTTGAACGA AGAATGGACA ATTCCAGAGA ATGGAGGACA GAGCAAAGCT 
GGATGGACTC CATTTGCTGG TAGAAAAGTG TTTGGAAAAG TGCACAATGT GATCATCAGA 
GGAGAAGAAG CTGTTATTGA TGGAAGAATC GTTGCAATTC CAGGATTCGG AAAGAATGTC 
CGTCTTTATC CACACTCTGG AACTGCTCAT CGTGGAGATT CAGACTTCGA TCAAATCTTG 
GAACCAATTC CTCAACAAAT GATTGAAAGT TCTTCAGATG AACAAAGTCC ACTTCATACT 
CCACCTAGAG CTCATACGCC GATTGCATTC CCTGGAGAGC TTCTTGCCAA GAATTGTATC 
AGTGTGAAGC ATCTTGATAA GGGCCAGATC AATCGTATTT TCGAGTTGGC GGACCGATAC 
AAGCACGATG TCGAAAAGGG CCACCCATTG ACACATATTC TGAATGGAAA AGTATTGGTC 
AATCTATTCT ACGAAGTGTC AACAAGAACA TCTTGCTCGT TCTCTGCAGC CATGCAACGT 
CTTGGAGGAT CTGTCATCTC TGTTGATAGT CAATCATCTT CAGTTCAAAA GGGAGAAACT 
TTGGAAGACA CAGTGCAGGT TTTGGGAAGT TACGGAGATA TTCTGGTTCT GAGAAGTAAT 
GAGAATGGAG CTGCTGATAG AGCTGCTCGA GTTTGCGATC AACCGGTCAT CAATGGAGGT 
GATGGAACTG GTGAACATCC AACTCAAGCT CTTCTCGATG TGTACACTAT TCGACAAGAA 
ATGGGAACAG TCAATGGACT TACCATCGCT TTGGTTGGAG ATTTGAAGAA TGGAAGAACG 
GTTCACTCAT TGGCTAAACT TTTATGTTTG TATAAGGACA TCACCCTTCA CTACGTTGCT 
CCGAGTACCG AACTTGAAAT GCCACAGGAA GTCTTGGACT ACGTCTCGTC GAAGTCGAAT 
TTTGTTCAGA AGAAGTTCAC CAGTCTGGCT GAAGGAATCA ATCATGTCGA CGTTGTCTAC 
GTGACAAGAA TTCAAAAAGA ACGATTCTCA TCTCCAGATG AATACAATAA GGTGAAAGGA 
AGCTACGTAA TCAATGCGAA ACTTCTCAAT GAGGCCGCCA GAGATGTCGA GGAACCATCG 
AGTCTGTTGG TTCCAGCTCG TTCTCTTCCA ATCGTCATGC ATCCATTGCC TCGTGTGGAT 
GAGATTGCTG TTGAACTGGA TCATGATGAA AGAGCCGCCT ACTTCAGACA AGCAAAGAAT 
GGGGTCTTCG TTCGTATGTC TATTCTATCC CTTCTTCTTG GAAGAGGGCA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pyr-1 ( NM_063437.5 ) cDNA ORF clone, Caenorhabditis elegans -> NP_495838.2 Caenorhabditis elegans Glutamine-dependent carbamoyl-phosphate synthase (pyr-1), mRNA.

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CloneID	OCa337543
Clone ID Related Accession (Same CDS sequence)	NM_063437.4 , NM_063437.5
Accession Version	NM_063437.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Glutamine-dependent carbamoyl-phosphate synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_063437.4.

ATGAGAGCGA CTTTGCATCT CGAGGACGGG TCCACTTTTG TGGGCTCCAT TTATGGAGCA 
ACCAAAAGTG TAGTTGGAGA AATTGTTTTT CAAACCGGAA TGGTTGGTTA TGTGGAATCT 
CTTACCGATC CATCATACGC CAAGCAGCTT CTCACACTTA CATATCCACT TATTGGAAAT 
TACGGAGTTC CATCTGCAGA GATTCTGGAT CAGTTCAAGC TACCAGCTGA ATTTGAGTCT 
GACAGGATCT GGCCAGCAGC TCTCATTGTT GAGAAAATTT GTGTCGATGG AGAACACAGT 
CATTGGCAAG CAGTTCAGTC GTTATCAGAA TGGCTCAGAA AAGCTGATGT TCCATGTCTT 
TCCGGAATTG ATGTTCGCCA ATTGGTGAAG AAAATCAGAG AGACTGGAAC AATGAAGGCG 
AAGCTTGTGA TCGAATCAGA CAATGCACAA AACTTCGACT ACGTTGACGT CAATGCTGAA 
AATTTGGTTG ACTTCGTGTC GAGAAAAGAG CCAGTTGTCT ATGGATCCGG TGATCAAACC 
ATTCTCGCAG TTGATTGTGG TCTCAAGAAT AATCAAATTA GATGTCTCGC AAAACGAGGA 
TTTCGTGTCA AAGTGGTTCC TTGGAATCAT CCAATTGATA CTGAAAGTGA TTATGATGGA 
CTTTTCCTTT CCAACGGACC AGGAGATCCA GAAATTTGTG CACCACTGGT CGATCGATTA 
GCCAAAGTTA TTGCAAGAGG AGACAAACCA ATTTTTGGAA TTTGTCTCGG ACACCAAATT 
TTGTCTAGAG CTATCGGAGC GAAAACCTAC AAATTGAAAT ACGGTAACCG TGGTCACAAT 
CAGCCATGCA CTCATTATGC TACTGGCCGT TGCTACATCA CTTCTCAAAA TCATGGATAC 
GCAGTTGATC CAGATTCACT TCCAGCTGAT TGGAAGGCAC TTTTCACGAA TGAGAATGAC 
AAGACCAACG AAGGAATTGT GCACTCGTCT AAACCATTTT TCAGTGTTCA ATTCCATCCA 
GAACACACTG CCGGGCCAAC TGATTGCGAG TTTTTGTTCG ATGTTTTTGC TGATTCTGTG 
AGACAAGCAA AGAGTGGCAC ATTCATGAAT GTCGATCAAG AGCTCACTCG TCTTATGACT 
TTCACTCCCA TCTATCATGC CAAAGAACAG AGAAAAGTTC TCGTTCTCGG ATCAGGCGGT 
CTCACTATTG GTCAAGCTGG AGAGTTTGAT TATTCTGGGG CTCAAGCTCT GAAGGCATTG 
AGAGAAGAAG GAATTCGAAC AGTTCTCATC AACCCAAACA TTGCTACAGT TCAAACCTCC 
AAAGGCTTTG CTGATTTCAC TTACTTCCTT CCAATCACTA AAGAATACGT TACCGATGTG 
ATCAAGAAGG AGCGACCAAC TGGAATCTTG TGTACCTTTG GAGGTCAAAC TGCTCTCAAC 
TGCGCCATCG ACTTGTATAA AGATGGAATT TTTGAGCAAT ACGATGTGCA GGTTCTTGGA 
ACTCAAATCA ACACAATTAT GAAAACAGAA GATCGCGATT TGTTCAATCA AGAAATTTCG 
GCTATTGGAG AAAAGGTTGC ACCAAGTAAA GCAGCGACGA CCATGGAAGG TGCCATTGAA 
GCAGCTGAAG AGTTGGGTTA TCCAGTTCTT GTCAGAGCCG CATATGCTCT TGGTGGACTT 
GGATCAGGAT TCGCTGACAA CCGGGAAGAG CTCATTGCAA TTGCTCAACA AGCTTTAGCA 
CACTCAAATC AGGTTCTTGT TGATAAGAGT TTGAAAGGAT GGAAGGAAGT GGAATATGAA 
GTTGTTCGGG ACGCTTACGA TAATTGTATT ACCGTTTGCA ACATGGAAAA TGTGGATCCA 
CTTGGAATTC ATACTGGTGA AAGTGTTGTT GTTGCACCAT CTCAAACTCT TTCTGACAGA 
GAATACAATG CTCTTAGAAC ATGTGCAATC AAAGTTATTC GGCACTTGGG AATTATTGGA 
GAGTGTAATA TTCAATACGC TCTGGATCCG TATTCCTTGA CTTATTACAT TATCGAAGTC 
AATGCACGAC TTTCTCGTTC TTCTGCTCTT GCCTCAAAAG CCACCGGATA CCCATTGGCA 
TATGTTGCTG CAAAGTTGGC ATTGGGACAA CATCTGCCAG TCATCCGAAA TAGTGTCACT 
GGAACCACAA CTGCATGCTT TGAGCCAAGT TTGGACTATT GTGTTGTGAA GATTCCTCGT 
TGGGATCTTG GAAAATTCGC TCGTGTCAGC ACACAAATTG GAAGCTCTAT GAAATCAGTT 
GGAGAAGTCA TGGGAATCGG GAGATGCTTT GAAGAGGCTC TTCAGAAGGC GTTGAGAATG 
GTGTCCGATC ATGCTGACGG TTTCTCTCCA TACACATTCA GTCGTCCTAC AACAGCTGAT 
GATCTTTCAA AGCCTACAGA TAAGAGAATG TTTGCATTGG CACGTGGAAT GTATTATGGA 
GACTTTGATG TTGAAAAAGC TCACGAGCTG ACACGTATTG ATCGTTGGTT CTTGTTCAGA 
ATGCAGAACA TCGTTGATAT TTACCATCGC CTCGAAAAGA CTGACGTCAA TACAGTATCT 
GCTGAACTGC TCCTGGAGGC GAAACAAGCA GGATTCTCCG ACAGACAAAT AGCAAAGAAA 
ATCGGAAGCA ACGAATACAC AGTTCGCGAA GCTCGTTTCG TCAAAGGAAT CACTCCTTGC 
GTGAAGCAAA TTGACACAGT TGCTGGAGAA TGGCCTGCTC AGACAAACTA CCTCTACACA 
ACATTCAATG GAATAGAGAA TGATGTTTCA TTCAATATGA AGAACGCTGT GATGGTTCTC 
GGATCAGGAG TATACCGAAT CGGTTCTTCA GTTGAATTTG ATTCTTCTTG CGTTGGATGC 
ATCAGAGAGT TGAAGGCTTT GGGATACTCT ACAATCACCG TCAATTGCAA TCCTGAAACT 
GTTTCTACGG ATTATGATAT TTGTGATCGA TTGTATTTTG AAGAGATTTC TTTTGAAACC 
GTTCTCGACG TTTATCATTT GGAAAAACCA AAGGGAGTGA TTCTGGCATT TGGAGGTCAA 
GCACCGAATA ACATTGCGAT GAGCTTGAGC AGAGCTCAGG TGAAGATCTT TGGAACTTCA 
CCAAACGACA TTGATAACGC AGAAGATAGA TTCAAGTTCT CAAGAAAATT GGAATCTTTG 
AAAATCAGCC AGCCACAATG GAAGAAAAGC GAAAATATGG AAGATGCTAA GAACTTCTGT 
GCTCAAGTTG GATATCCATG CTTGATCCGT CCTTCATATG TTTTATCTGG TGCAGCAATG 
AATGTGGCTC ACAACGCTGA AGACTTGGAA GTCTTTCTCA AGCAAGCTGC TGTGGTCGCC 
AAGGAGCATC CAGTTGTTGT GAGCAAATTT ATCAATGAAG CGAAGGAATT GGATGTAGAT 
GCAGTTGCCC TTGATGGAAA ACTCGTTGTA ATGGCTGTCA GTGAACATAT TGAAAATGCA 
GGTGTTCATT CGGGAGATGC TACTCTTGTC ACTCCAGCTC AGGATATGAA CAAACTGACA 
TTGGATAGAA TCAAGGATAT CACTTTCAGA ATCGCTGAAG CATTTAATGT CAACGGACCT 
TTCAATATGC AGCTCATTGC TAAAAATAAC GAACTCAAAG TTATTGAATG CAACTTGCGA 
GTATCCAGAT CATTCCCGTT CGTCTCAAAA ACTCTCGATT ACGATTTTGT GGCTTTGGCT 
ACTCGTGCAA TGATGGCTTC AGATTCACCT GCAATTCGAG CTACAATCAA ACCAACAGCC 
ACCTTGCTCA AAGGAAAGGG AAGAGTCGGA GTGAAGGTTC CTCAATTCTC TTTCTCAAGA 
CTCGCTGGTG CCGATGTGAT GCTTGGTGTC GAGATGGCTT CAACTGGAGA AGTTGCTTGC 
TTCGGAACTT CAAGATGCGA TGCCTACCTC AAAGCTTTAC TCTCCACTGG TTTCGTTGTT 
CCAAAACAAA ATATTTTCAT TTCCATTGGA GGATATCACG CGAAGGCTGA GATGCTCAAG 
TCTGTAGAAG CATTGCTGAA GTTGGGATAC GAATTGTATG GATCCAAAGG AACTGCCGAC 
TATTTCCAAT CGAATAAGAT CAATGTGAAG CCAGTCGATT GGCCTTTTGA AGAAGGATCG 
TCCGATGAGA AAACTGCAAG TGGAACAAGA TCTGTAGTTG AATTCCTCGA GAACAAAGAA 
TTCCACTTGG TCATCAACCT ACCGATTCGT GGATCAGGAG CATACCGAGT CTCTGCCTTC 
CGCACTCATG GTTACAAGAC GAGAAGGATG GCAATTGACA ATGGAATTCC CTTGATAACT 
GATATTAAGT GTGCTAAGAC CTTCATTCAA GCCCTTGAAA TGGTTGGAAA ACGTCCAACT 
ATGAATTCAT TGGTCGATTG TGTGACTTCC AAGAGTTTGA AGCGTTTGCC AGGAATGGTT 
GATATTCATG TACATGTTAG AGAACCAGGA GCAACACACA AAGAAGATTG GGCAACTTGT 
TCAAAGGCTG CACTAGCTGG AGGTGTTACT ACAATTTTGG CAATGCCAAA CACTTCTCCT 
GTTCTTGTGG ATACTGATTC CTTCTATCAA ACGGAACAGT TGGCTTCTGC AAAATCGGTC 
GTCGACTACG CCCTCTACAT CGGAGCTACT CCAAACAATT CGAAGTTTGC AGCGGAGTTT 
GCTGACAAAG CCGCTGGCTT GAAGATGTAT CTCAATGAGA CATTCAGTAC TCTGAAGATG 
GATAATATTT CAGACTGGGC TAAGCACCTG AGTGCCTTCC CTGCTAATCG TCCGATCGTC 
TGTCACGCCG AGAAGCAAAC ACTTGCCGCC ATTCTTTGTA TGGCTCAGAT GGCGAATAGA 
GCAGTTCACA TTGCCCACGT CGCTACGGCT GATGAGATTA ATCTCGTCAA GGAAGCTAAA 
CAAAGAGGAT GGAATGTTAC CTGTGAAGTC TGCCCACATC ATCTTTTCCT CATTGAAGAA 
GATCTTCCTG ATGGAATTCG TGAAGTTCGT CCACGTCTCG TAAAACCAGA AGATCGTCAG 
GCTCTTTGGG ACAACATGGA ATACATTGAT TGTTTTGCAA CTGATCATGC ACCTCATACG 
TGGGCTGAGA AGACTGGAAA AGATGGGAAA ATTCCACCAG GCTTCCCTGG AGTTGAGTAC 
ATGCTCCCAC TTCTTCTTAC AGCTGTGCAT GATGGTAAAT TGACCATGAA GGAACTCACT 
GATCGTATGT CCACCAATCC TCGTCGAATT TTCAACTTGC CTCCACAAGA TGATACCTAC 
ATTGAGGTTG ATTTGAACGA AGAATGGACA ATTCCAGAGA ATGGAGGACA GAGCAAAGCT 
GGATGGACTC CATTTGCTGG TAGAAAAGTG TTTGGAAAAG TGCACAATGT GATCATCAGA 
GGAGAAGAAG CTGTTATTGA TGGAAGAATC GTTGCAATTC CAGGATTCGG AAAGAATGTC 
CGTCTTTATC CACACTCTGG AACTGCTCAT CGTGGAGATT CAGACTTCGA TCAAATCTTG 
GAACCAATTC CTCAACAAAT GATTGAAAGT TCTTCAGATG AACAAAGTCC ACTTCATACT 
CCACCTAGAG CTCATACGCC GATTGCATTC CCTGGAGAGC TTCTTGCCAA GAATTGTATC 
AGTGTGAAGC ATCTTGATAA GGGCCAGATC AATCGTATTT TCGAGTTGGC GGACCGATAC 
AAGCACGATG TCGAAAAGGG CCACCCATTG ACACATATTC TGAATGGAAA AGTATTGGTC 
AATCTATTCT ACGAAGTGTC AACAAGAACA TCTTGCTCGT TCTCTGCAGC CATGCAACGT 
CTTGGAGGAT CTGTCATCTC TGTTGATAGT CAATCATCTT CAGTTCAAAA GGGAGAAACT 
TTGGAAGACA CAGTGCAGGT TTTGGGAAGT TACGGAGATA TTCTGGTTCT GAGAAGTAAT 
GAGAATGGAG CTGCTGATAG AGCTGCTCGA GTTTGCGATC AACCGGTCAT CAATGGAGGT 
GATGGAACTG GTGAACATCC AACTCAAGCT CTTCTCGATG TGTACACTAT TCGACAAGAA 
ATGGGAACAG TCAATGGACT TACCATCGCT TTGGTTGGAG ATTTGAAGAA TGGAAGAACG 
GTTCACTCAT TGGCTAAACT TTTATGTTTG TATAAGGACA TCACCCTTCA CTACGTTGCT 
CCGAGTACCG AACTTGAAAT GCCACAGGAA GTCTTGGACT ACGTCTCGTC GAAGTCGAAT 
TTTGTTCAGA AGAAGTTCAC CAGTCTGGCT GAAGGAATCA ATCATGTCGA CGTTGTCTAC 
GTGACAAGAA TTCAAAAAGA ACGATTCTCA TCTCCAGATG AATACAATAA GGTGAAAGGA 
AGCTACGTAA TCAATGCGAA ACTTCTCAAT GAGGCCGCCA GAGATGTCGA GGAACCATCG 
AGTCTGTTGG TTCCAGCTCG TTCTCTTCCA ATCGTCATGC ATCCATTGCC TCGTGTGGAT 
GAGATTGCTG TTGAACTGGA TCATGATGAA AGAGCCGCCT ACTTCAGACA AGCAAAGAAT 
GGGGTCTTCG TTCGTATGTC TATTCTATCC CTTCTTCTTG GAAGAGGGCA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_495838.2
CDS	26..6622
Translation	MRATLHLEDGSTFVGSIYGATKSVVGEIVFQTGMVGYVESLTDPSYAKQLLTLTYPLIGNYGVPSAEILDQFKLPAEFESDRIWPAALIVEKICVDGEHSHWQAVQSLSEWLRKADVPCLSGIDVRQLVKKIRETGTMKAKLVIESDNAQNFDYVDVNAENLVDFVSRKEPVVYGSGDQTILAVDCGLKNNQIRCLAKRGFRVKVVPWNHPIDTESDYDGLFLSNGPGDPEICAPLVDRLAKVIARGDKPIFGICLGHQILSRAIGAKTYKLKYGNRGHNQPCTHYATGRCYITSQNHGYAVDPDSLPADWKALFTNENDKTNEGIVHSSKPFFSVQFHPEHTAGPTDCEFLFDVFADSVRQAKSGTFMNVDQELTRLMTFTPIYHAKEQRKVLVLGSGGLTIGQAGEFDYSGAQALKALREEGIRTVLINPNIATVQTSKGFADFTYFLPITKEYVTDVIKKERPTGILCTFGGQTALNCAIDLYKDGIFEQYDVQVLGTQINTIMKTEDRDLFNQEISAIGEKVAPSKAATTMEGAIEAAEELGYPVLVRAAYALGGLGSGFADNREELIAIAQQALAHSNQVLVDKSLKGWKEVEYEVVRDAYDNCITVCNMENVDPLGIHTGESVVVAPSQTLSDREYNALRTCAIKVIRHLGIIGECNIQYALDPYSLTYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGQHLPVIRNSVTGTTTACFEPSLDYCVVKIPRWDLGKFARVSTQIGSSMKSVGEVMGIGRCFEEALQKALRMVSDHADGFSPYTFSRPTTADDLSKPTDKRMFALARGMYYGDFDVEKAHELTRIDRWFLFRMQNIVDIYHRLEKTDVNTVSAELLLEAKQAGFSDRQIAKKIGSNEYTVREARFVKGITPCVKQIDTVAGEWPAQTNYLYTTFNGIENDVSFNMKNAVMVLGSGVYRIGSSVEFDSSCVGCIRELKALGYSTITVNCNPETVSTDYDICDRLYFEEISFETVLDVYHLEKPKGVILAFGGQAPNNIAMSLSRAQVKIFGTSPNDIDNAEDRFKFSRKLESLKISQPQWKKSENMEDAKNFCAQVGYPCLIRPSYVLSGAAMNVAHNAEDLEVFLKQAAVVAKEHPVVVSKFINEAKELDVDAVALDGKLVVMAVSEHIENAGVHSGDATLVTPAQDMNKLTLDRIKDITFRIAEAFNVNGPFNMQLIAKNNELKVIECNLRVSRSFPFVSKTLDYDFVALATRAMMASDSPAIRATIKPTATLLKGKGRVGVKVPQFSFSRLAGADVMLGVEMASTGEVACFGTSRCDAYLKALLSTGFVVPKQNIFISIGGYHAKAEMLKSVEALLKLGYELYGSKGTADYFQSNKINVKPVDWPFEEGSSDEKTASGTRSVVEFLENKEFHLVINLPIRGSGAYRVSAFRTHGYKTRRMAIDNGIPLITDIKCAKTFIQALEMVGKRPTMNSLVDCVTSKSLKRLPGMVDIHVHVREPGATHKEDWATCSKAALAGGVTTILAMPNTSPVLVDTDSFYQTEQLASAKSVVDYALYIGATPNNSKFAAEFADKAAGLKMYLNETFSTLKMDNISDWAKHLSAFPANRPIVCHAEKQTLAAILCMAQMANRAVHIAHVATADEINLVKEAKQRGWNVTCEVCPHHLFLIEEDLPDGIREVRPRLVKPEDRQALWDNMEYIDCFATDHAPHTWAEKTGKDGKIPPGFPGVEYMLPLLLTAVHDGKLTMKELTDRMSTNPRRIFNLPPQDDTYIEVDLNEEWTIPENGGQSKAGWTPFAGRKVFGKVHNVIIRGEEAVIDGRIVAIPGFGKNVRLYPHSGTAHRGDSDFDQILEPIPQQMIESSSDEQSPLHTPPRAHTPIAFPGELLAKNCISVKHLDKGQINRIFELADRYKHDVEKGHPLTHILNGKVLVNLFYEVSTRTSCSFSAAMQRLGGSVISVDSQSSSVQKGETLEDTVQVLGSYGDILVLRSNENGAADRAARVCDQPVINGGDGTGEHPTQALLDVYTIRQEMGTVNGLTIALVGDLKNGRTVHSLAKLLCLYKDITLHYVAPSTELEMPQEVLDYVSSKSNFVQKKFTSLAEGINHVDVVYVTRIQKERFSSPDEYNKVKGSYVINAKLLNEAARDVEEPSSLLVPARSLPIVMHPLPRVDEIAVELDHDERAAYFRQAKNGVFVRMSILSLLLGRGHL

Target ORF information:

RefSeq Version	NM_063437.5
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Glutamine-dependent carbamoyl-phosphate synthase (pyr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_063437.5

ORF Insert Sequence:

ATGAGAGCGA CTTTGCATCT CGAGGACGGG TCCACTTTTG TGGGCTCCAT TTATGGAGCA 
ACCAAAAGTG TAGTTGGAGA AATTGTTTTT CAAACCGGAA TGGTTGGTTA TGTGGAATCT 
CTTACCGATC CATCATACGC CAAGCAGCTT CTCACACTTA CATATCCACT TATTGGAAAT 
TACGGAGTTC CATCTGCAGA GATTCTGGAT CAGTTCAAGC TACCAGCTGA ATTTGAGTCT 
GACAGGATCT GGCCAGCAGC TCTCATTGTT GAGAAAATTT GTGTCGATGG AGAACACAGT 
CATTGGCAAG CAGTTCAGTC GTTATCAGAA TGGCTCAGAA AAGCTGATGT TCCATGTCTT 
TCCGGAATTG ATGTTCGCCA ATTGGTGAAG AAAATCAGAG AGACTGGAAC AATGAAGGCG 
AAGCTTGTGA TCGAATCAGA CAATGCACAA AACTTCGACT ACGTTGACGT CAATGCTGAA 
AATTTGGTTG ACTTCGTGTC GAGAAAAGAG CCAGTTGTCT ATGGATCCGG TGATCAAACC 
ATTCTCGCAG TTGATTGTGG TCTCAAGAAT AATCAAATTA GATGTCTCGC AAAACGAGGA 
TTTCGTGTCA AAGTGGTTCC TTGGAATCAT CCAATTGATA CTGAAAGTGA TTATGATGGA 
CTTTTCCTTT CCAACGGACC AGGAGATCCA GAAATTTGTG CACCACTGGT CGATCGATTA 
GCCAAAGTTA TTGCAAGAGG AGACAAACCA ATTTTTGGAA TTTGTCTCGG ACACCAAATT 
TTGTCTAGAG CTATCGGAGC GAAAACCTAC AAATTGAAAT ACGGTAACCG TGGTCACAAT 
CAGCCATGCA CTCATTATGC TACTGGCCGT TGCTACATCA CTTCTCAAAA TCATGGATAC 
GCAGTTGATC CAGATTCACT TCCAGCTGAT TGGAAGGCAC TTTTCACGAA TGAGAATGAC 
AAGACCAACG AAGGAATTGT GCACTCGTCT AAACCATTTT TCAGTGTTCA ATTCCATCCA 
GAACACACTG CCGGGCCAAC TGATTGCGAG TTTTTGTTCG ATGTTTTTGC TGATTCTGTG 
AGACAAGCAA AGAGTGGCAC ATTCATGAAT GTCGATCAAG AGCTCACTCG TCTTATGACT 
TTCACTCCCA TCTATCATGC CAAAGAACAG AGAAAAGTTC TCGTTCTCGG ATCAGGCGGT 
CTCACTATTG GTCAAGCTGG AGAGTTTGAT TATTCTGGGG CTCAAGCTCT GAAGGCATTG 
AGAGAAGAAG GAATTCGAAC AGTTCTCATC AACCCAAACA TTGCTACAGT TCAAACCTCC 
AAAGGCTTTG CTGATTTCAC TTACTTCCTT CCAATCACTA AAGAATACGT TACCGATGTG 
ATCAAGAAGG AGCGACCAAC TGGAATCTTG TGTACCTTTG GAGGTCAAAC TGCTCTCAAC 
TGCGCCATCG ACTTGTATAA AGATGGAATT TTTGAGCAAT ACGATGTGCA GGTTCTTGGA 
ACTCAAATCA ACACAATTAT GAAAACAGAA GATCGCGATT TGTTCAATCA AGAAATTTCG 
GCTATTGGAG AAAAGGTTGC ACCAAGTAAA GCAGCGACGA CCATGGAAGG TGCCATTGAA 
GCAGCTGAAG AGTTGGGTTA TCCAGTTCTT GTCAGAGCCG CATATGCTCT TGGTGGACTT 
GGATCAGGAT TCGCTGACAA CCGGGAAGAG CTCATTGCAA TTGCTCAACA AGCTTTAGCA 
CACTCAAATC AGGTTCTTGT TGATAAGAGT TTGAAAGGAT GGAAGGAAGT GGAATATGAA 
GTTGTTCGGG ACGCTTACGA TAATTGTATT ACCGTTTGCA ACATGGAAAA TGTGGATCCA 
CTTGGAATTC ATACTGGTGA AAGTGTTGTT GTTGCACCAT CTCAAACTCT TTCTGACAGA 
GAATACAATG CTCTTAGAAC ATGTGCAATC AAAGTTATTC GGCACTTGGG AATTATTGGA 
GAGTGTAATA TTCAATACGC TCTGGATCCG TATTCCTTGA CTTATTACAT TATCGAAGTC 
AATGCACGAC TTTCTCGTTC TTCTGCTCTT GCCTCAAAAG CCACCGGATA CCCATTGGCA 
TATGTTGCTG CAAAGTTGGC ATTGGGACAA CATCTGCCAG TCATCCGAAA TAGTGTCACT 
GGAACCACAA CTGCATGCTT TGAGCCAAGT TTGGACTATT GTGTTGTGAA GATTCCTCGT 
TGGGATCTTG GAAAATTCGC TCGTGTCAGC ACACAAATTG GAAGCTCTAT GAAATCAGTT 
GGAGAAGTCA TGGGAATCGG GAGATGCTTT GAAGAGGCTC TTCAGAAGGC GTTGAGAATG 
GTGTCCGATC ATGCTGACGG TTTCTCTCCA TACACATTCA GTCGTCCTAC AACAGCTGAT 
GATCTTTCAA AGCCTACAGA TAAGAGAATG TTTGCATTGG CACGTGGAAT GTATTATGGA 
GACTTTGATG TTGAAAAAGC TCACGAGCTG ACACGTATTG ATCGTTGGTT CTTGTTCAGA 
ATGCAGAACA TCGTTGATAT TTACCATCGC CTCGAAAAGA CTGACGTCAA TACAGTATCT 
GCTGAACTGC TCCTGGAGGC GAAACAAGCA GGATTCTCCG ACAGACAAAT AGCAAAGAAA 
ATCGGAAGCA ACGAATACAC AGTTCGCGAA GCTCGTTTCG TCAAAGGAAT CACTCCTTGC 
GTGAAGCAAA TTGACACAGT TGCTGGAGAA TGGCCTGCTC AGACAAACTA CCTCTACACA 
ACATTCAATG GAATAGAGAA TGATGTTTCA TTCAATATGA AGAACGCTGT GATGGTTCTC 
GGATCAGGAG TATACCGAAT CGGTTCTTCA GTTGAATTTG ATTCTTCTTG CGTTGGATGC 
ATCAGAGAGT TGAAGGCTTT GGGATACTCT ACAATCACCG TCAATTGCAA TCCTGAAACT 
GTTTCTACGG ATTATGATAT TTGTGATCGA TTGTATTTTG AAGAGATTTC TTTTGAAACC 
GTTCTCGACG TTTATCATTT GGAAAAACCA AAGGGAGTGA TTCTGGCATT TGGAGGTCAA 
GCACCGAATA ACATTGCGAT GAGCTTGAGC AGAGCTCAGG TGAAGATCTT TGGAACTTCA 
CCAAACGACA TTGATAACGC AGAAGATAGA TTCAAGTTCT CAAGAAAATT GGAATCTTTG 
AAAATCAGCC AGCCACAATG GAAGAAAAGC GAAAATATGG AAGATGCTAA GAACTTCTGT 
GCTCAAGTTG GATATCCATG CTTGATCCGT CCTTCATATG TTTTATCTGG TGCAGCAATG 
AATGTGGCTC ACAACGCTGA AGACTTGGAA GTCTTTCTCA AGCAAGCTGC TGTGGTCGCC 
AAGGAGCATC CAGTTGTTGT GAGCAAATTT ATCAATGAAG CGAAGGAATT GGATGTAGAT 
GCAGTTGCCC TTGATGGAAA ACTCGTTGTA ATGGCTGTCA GTGAACATAT TGAAAATGCA 
GGTGTTCATT CGGGAGATGC TACTCTTGTC ACTCCAGCTC AGGATATGAA CAAACTGACA 
TTGGATAGAA TCAAGGATAT CACTTTCAGA ATCGCTGAAG CATTTAATGT CAACGGACCT 
TTCAATATGC AGCTCATTGC TAAAAATAAC GAACTCAAAG TTATTGAATG CAACTTGCGA 
GTATCCAGAT CATTCCCGTT CGTCTCAAAA ACTCTCGATT ACGATTTTGT GGCTTTGGCT 
ACTCGTGCAA TGATGGCTTC AGATTCACCT GCAATTCGAG CTACAATCAA ACCAACAGCC 
ACCTTGCTCA AAGGAAAGGG AAGAGTCGGA GTGAAGGTTC CTCAATTCTC TTTCTCAAGA 
CTCGCTGGTG CCGATGTGAT GCTTGGTGTC GAGATGGCTT CAACTGGAGA AGTTGCTTGC 
TTCGGAACTT CAAGATGCGA TGCCTACCTC AAAGCTTTAC TCTCCACTGG TTTCGTTGTT 
CCAAAACAAA ATATTTTCAT TTCCATTGGA GGATATCACG CGAAGGCTGA GATGCTCAAG 
TCTGTAGAAG CATTGCTGAA GTTGGGATAC GAATTGTATG GATCCAAAGG AACTGCCGAC 
TATTTCCAAT CGAATAAGAT CAATGTGAAG CCAGTCGATT GGCCTTTTGA AGAAGGATCG 
TCCGATGAGA AAACTGCAAG TGGAACAAGA TCTGTAGTTG AATTCCTCGA GAACAAAGAA 
TTCCACTTGG TCATCAACCT ACCGATTCGT GGATCAGGAG CATACCGAGT CTCTGCCTTC 
CGCACTCATG GTTACAAGAC GAGAAGGATG GCAATTGACA ATGGAATTCC CTTGATAACT 
GATATTAAGT GTGCTAAGAC CTTCATTCAA GCCCTTGAAA TGGTTGGAAA ACGTCCAACT 
ATGAATTCAT TGGTCGATTG TGTGACTTCC AAGAGTTTGA AGCGTTTGCC AGGAATGGTT 
GATATTCATG TACATGTTAG AGAACCAGGA GCAACACACA AAGAAGATTG GGCAACTTGT 
TCAAAGGCTG CACTAGCTGG AGGTGTTACT ACAATTTTGG CAATGCCAAA CACTTCTCCT 
GTTCTTGTGG ATACTGATTC CTTCTATCAA ACGGAACAGT TGGCTTCTGC AAAATCGGTC 
GTCGACTACG CCCTCTACAT CGGAGCTACT CCAAACAATT CGAAGTTTGC AGCGGAGTTT 
GCTGACAAAG CCGCTGGCTT GAAGATGTAT CTCAATGAGA CATTCAGTAC TCTGAAGATG 
GATAATATTT CAGACTGGGC TAAGCACCTG AGTGCCTTCC CTGCTAATCG TCCGATCGTC 
TGTCACGCCG AGAAGCAAAC ACTTGCCGCC ATTCTTTGTA TGGCTCAGAT GGCGAATAGA 
GCAGTTCACA TTGCCCACGT CGCTACGGCT GATGAGATTA ATCTCGTCAA GGAAGCTAAA 
CAAAGAGGAT GGAATGTTAC CTGTGAAGTC TGCCCACATC ATCTTTTCCT CATTGAAGAA 
GATCTTCCTG ATGGAATTCG TGAAGTTCGT CCACGTCTCG TAAAACCAGA AGATCGTCAG 
GCTCTTTGGG ACAACATGGA ATACATTGAT TGTTTTGCAA CTGATCATGC ACCTCATACG 
TGGGCTGAGA AGACTGGAAA AGATGGGAAA ATTCCACCAG GCTTCCCTGG AGTTGAGTAC 
ATGCTCCCAC TTCTTCTTAC AGCTGTGCAT GATGGTAAAT TGACCATGAA GGAACTCACT 
GATCGTATGT CCACCAATCC TCGTCGAATT TTCAACTTGC CTCCACAAGA TGATACCTAC 
ATTGAGGTTG ATTTGAACGA AGAATGGACA ATTCCAGAGA ATGGAGGACA GAGCAAAGCT 
GGATGGACTC CATTTGCTGG TAGAAAAGTG TTTGGAAAAG TGCACAATGT GATCATCAGA 
GGAGAAGAAG CTGTTATTGA TGGAAGAATC GTTGCAATTC CAGGATTCGG AAAGAATGTC 
CGTCTTTATC CACACTCTGG AACTGCTCAT CGTGGAGATT CAGACTTCGA TCAAATCTTG 
GAACCAATTC CTCAACAAAT GATTGAAAGT TCTTCAGATG AACAAAGTCC ACTTCATACT 
CCACCTAGAG CTCATACGCC GATTGCATTC CCTGGAGAGC TTCTTGCCAA GAATTGTATC 
AGTGTGAAGC ATCTTGATAA GGGCCAGATC AATCGTATTT TCGAGTTGGC GGACCGATAC 
AAGCACGATG TCGAAAAGGG CCACCCATTG ACACATATTC TGAATGGAAA AGTATTGGTC 
AATCTATTCT ACGAAGTGTC AACAAGAACA TCTTGCTCGT TCTCTGCAGC CATGCAACGT 
CTTGGAGGAT CTGTCATCTC TGTTGATAGT CAATCATCTT CAGTTCAAAA GGGAGAAACT 
TTGGAAGACA CAGTGCAGGT TTTGGGAAGT TACGGAGATA TTCTGGTTCT GAGAAGTAAT 
GAGAATGGAG CTGCTGATAG AGCTGCTCGA GTTTGCGATC AACCGGTCAT CAATGGAGGT 
GATGGAACTG GTGAACATCC AACTCAAGCT CTTCTCGATG TGTACACTAT TCGACAAGAA 
ATGGGAACAG TCAATGGACT TACCATCGCT TTGGTTGGAG ATTTGAAGAA TGGAAGAACG 
GTTCACTCAT TGGCTAAACT TTTATGTTTG TATAAGGACA TCACCCTTCA CTACGTTGCT 
CCGAGTACCG AACTTGAAAT GCCACAGGAA GTCTTGGACT ACGTCTCGTC GAAGTCGAAT 
TTTGTTCAGA AGAAGTTCAC CAGTCTGGCT GAAGGAATCA ATCATGTCGA CGTTGTCTAC 
GTGACAAGAA TTCAAAAAGA ACGATTCTCA TCTCCAGATG AATACAATAA GGTGAAAGGA 
AGCTACGTAA TCAATGCGAA ACTTCTCAAT GAGGCCGCCA GAGATGTCGA GGAACCATCG 
AGTCTGTTGG TTCCAGCTCG TTCTCTTCCA ATCGTCATGC ATCCATTGCC TCGTGTGGAT 
GAGATTGCTG TTGAACTGGA TCATGATGAA AGAGCCGCCT ACTTCAGACA AGCAAAGAAT 
GGGGTCTTCG TTCGTATGTC TATTCTATCC CTTCTTCTTG GAAGAGGGCA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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pyr-1 cDNA ORF clone, Caenorhabditis elegans

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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