CAD cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAD
Entrez Gene ID	266992
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms	cb456,si:dkey-221h15.3,wu:fc30c12,wu:fc33d01,wu:fc67g02
General protein information	Preferred Names carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Names CAD protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009294886.2	XP_009293161.1	CAD protein isoform X2
XM_009294885.3	XP_009293160.1	CAD protein isoform X1
NM_001308825.1	NP_001295754.1	CAD protein
XM_009294885.2	XP_009293160.1	CAD protein isoform X1
XM_009294886.3	XP_009293161.1	CAD protein isoform X2
XM_005160811.4	XP_005160868.1	CAD protein isoform X3
XM_005160811.3	XP_005160868.1	CAD protein isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish Bone and General Physiology Are Differently Affected by Hormones or Changes in Gravity.
Aceto J, Nourizadeh-Lillabadi R, Mar?e R, Dardenne N, Jeanray N, Wehenkel L, Alestr?m P, van Loon JJ, Muller M
PloS one10(6)e0126928(2015)

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.
Coxam B, Neyt C, Grassini DR, Le Guen L, Smith KA, Schulte-Merker S, Hogan BM
Developmental dynamics : an official publication of the American Association of Anatomists244(1)1-9(2015 Jan)

The role of the DNA damage response in zebrafish and cellular models of Diamond Blackfan anemia.
Danilova N, Bibikova E, Covey TM, Nathanson D, Dimitrova E, Konto Y, Lindgren A, Glader B, Radu CG, Sakamoto KM, Lin S
Disease models & mechanisms7(7)895-905(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest important posttranslational modifications requiring Cad as an unappreciated mechanism that regulates Notch/Vegf signaling during angiogenesis.
Title: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.

This study demonistrated that a novel role for carbamoyl phosphate synthetase 2 in cranial sensory circuit formation.
Title: Novel role for carbamoyl phosphate synthetase 2 in cranial sensory circuit formation.

an essential role for CAD in facilitating proliferation and differentiation events in a tissue-specific manner
Title: Analysis of the Zebrafish perplexed mutation reveals tissue-specific roles for de novo pyrimidine synthesis during development.

The following CAD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25122	NM_001308825.1 Latest version!	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa25121	XM_005160811.4 Latest version!	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa39598	XM_009294885.3 Latest version!	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa39599	XM_009294886.3 Latest version!	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa39598	XM_009294885.2	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa39599	XM_009294886.2	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa25121	XM_005160811.3	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAD ( NM_001308825.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001295754.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), mRNA.

Price & Availability↑

CloneID	ODa25122
Clone ID Related Accession (Same CDS sequence)	NM_001308825.1
Accession Version	NM_001308825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	CAD protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511178.6. On May 19, 2015 this sequence version replaced XM_005160812.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GDQH01000642.1, AY880246.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGA ATTGGCTCTA CCAGCAGAGG CATACGCTCA CCCTCCTCCC 
CTGGCCAGGA TCCTGTCACC TCAAGCTGGG CAACCGCAGA TCCTGCCTCA CCCTCAAACC 
TCCCCGCTGC TGCACCCGCT GGTGGGCCAG CACATCCTGT CTGTGCGCCA GTTCAGCAAG 
GAACAGATGT CACATCTGTT CAATGTGGCA CATACTCTTC GTCTCATGGT GCAGAAGGAA 
CGACCGTTGG ATATTTTGAA GGGTAAAGTC ATGGCCTCTA TGTTTTACGA GGTGAGCACT 
CGCACCAGCA GTTCATTCGC AGCAGCCATG CATCGTCTGG GAGGGTCTGT GGTGCATTTC 
TGCGAGTCCA CATCTTCAAC GCAGAAGGGC GAGTCTCTGG TGGACTCTGT GAACACCATG 
AGCTGCTATG CTGATGTCAT TGTTCTGCGG CACCCCATAC CAGGAGCTGT AGAGAGTGCG 
GCAAGACATT GTCGGCGTCC GGTGATCAAT GCTGGTGATG GGGTCGGAGA ACATCCAACT 
CAGGCCCTTC TGGACATCTT TACAATCCGA GAAGAGCTGG GAACGGTCAA TGGCATGACT 
ATTACCATGG TAGGCGACCT GAAACATGGG CGAACTGTGC ATTCTCTAGC TAGACTTCTC 
ACTCAGTACA GAATCACTTT ACGCTATGTG GCTCCAAAGA ATCTGAGCAT GCCTGCAGAA 
ATCATCGACT TTGTGGCCTC CAAGGGAATT AAACAGGAGG AGTTTAATAG CATTGAAGAA 
GCCCTTCCTG ACACTGACGT TCTGTACATG ACGAGAATAC AGAAAGAGCG GTTCTCTTCT 
GAAAAAGAGT ACAATGCGTG TTTTGGCCAG TTCATCCTAA CCCCACATAT CATGACGGGA 
GCCAAAAGAA AGATGGTGGT CATGCATCCT CTTCCCAGAG TCAACGAGAT CAGTGTGGAA 
GTGGACACAG ATCCTCGGGC TGCTTACTTC CGGCAGGCAG AGAACGGGAT GTATATTCGG 
ATGGCACTAC TGGCTACTGT ATTGGGGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295754.1
CDS	234..6926
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	NM_001308825.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308825.1

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGA ATTGGCTCTA CCAGCAGAGG CATACGCTCA CCCTCCTCCC 
CTGGCCAGGA TCCTGTCACC TCAAGCTGGG CAACCGCAGA TCCTGCCTCA CCCTCAAACC 
TCCCCGCTGC TGCACCCGCT GGTGGGCCAG CACATCCTGT CTGTGCGCCA GTTCAGCAAG 
GAACAGATGT CACATCTGTT CAATGTGGCA CATACTCTTC GTCTCATGGT GCAGAAGGAA 
CGACCGTTGG ATATTTTGAA GGGTAAAGTC ATGGCCTCTA TGTTTTACGA GGTGAGCACT 
CGCACCAGCA GTTCATTCGC AGCAGCCATG CATCGTCTGG GAGGGTCTGT GGTGCATTTC 
TGCGAGTCCA CATCTTCAAC GCAGAAGGGC GAGTCTCTGG TGGACTCTGT GAACACCATG 
AGCTGCTATG CTGATGTCAT TGTTCTGCGG CACCCCATAC CAGGAGCTGT AGAGAGTGCG 
GCAAGACATT GTCGGCGTCC GGTGATCAAT GCTGGTGATG GGGTCGGAGA ACATCCAACT 
CAGGCCCTTC TGGACATCTT TACAATCCGA GAAGAGCTGG GAACGGTCAA TGGCATGACT 
ATTACCATGG TAGGCGACCT GAAACATGGG CGAACTGTGC ATTCTCTAGC TAGACTTCTC 
ACTCAGTACA GAATCACTTT ACGCTATGTG GCTCCAAAGA ATCTGAGCAT GCCTGCAGAA 
ATCATCGACT TTGTGGCCTC CAAGGGAATT AAACAGGAGG AGTTTAATAG CATTGAAGAA 
GCCCTTCCTG ACACTGACGT TCTGTACATG ACGAGAATAC AGAAAGAGCG GTTCTCTTCT 
GAAAAAGAGT ACAATGCGTG TTTTGGCCAG TTCATCCTAA CCCCACATAT CATGACGGGA 
GCCAAAAGAA AGATGGTGGT CATGCATCCT CTTCCCAGAG TCAACGAGAT CAGTGTGGAA 
GTGGACACAG ATCCTCGGGC TGCTTACTTC CGGCAGGCAG AGAACGGGAT GTATATTCGG 
ATGGCACTAC TGGCTACTGT ATTGGGGCGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_005160811.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160868.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.

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CloneID	ODa25121
Clone ID Related Accession (Same CDS sequence)	XM_005160811.4 , XM_005160811.3
Accession Version	XM_005160811.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	CAD protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160811.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160868.1
CDS	627..7358
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPGFKRSTMHPQKAFELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	XM_005160811.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160811.4

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_005160811.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160868.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.

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CloneID	ODa25121
Clone ID Related Accession (Same CDS sequence)	XM_005160811.4 , XM_005160811.3
Accession Version	XM_005160811.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	CAD protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160868.1
CDS	637..7368
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPGFKRSTMHPQKAFELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	XM_005160811.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160811.3

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_009294885.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293160.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.

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CloneID	ODa39598
Clone ID Related Accession (Same CDS sequence)	XM_009294885.3 , XM_009294885.2
Accession Version	XM_009294885.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGCC 
GAAGAGGAAG TGTCTTTAAG AGCACCATTT GATGCAGAAT TGGCTCTACC AGCAGAGGCA 
TACGCTCACC CTCCTCCCCT GGCCAGGATC CTGTCACCTC AAGCTGGGCA ACCGCAGATC 
CTGCCTCACC CTCAAACCTC CCCGCTGCTG CACCCGCTGG TGGGCCAGCA CATCCTGTCT 
GTGCGCCAGT TCAGCAAGGA ACAGATGTCA CATCTGTTCA ATGTGGCACA TACTCTTCGT 
CTCATGGTGC AGAAGGAACG ACCGTTGGAT ATTTTGAAGG GTAAAGTCAT GGCCTCTATG 
TTTTACGAGG TGAGCACTCG CACCAGCAGT TCATTCGCAG CAGCCATGCA TCGTCTGGGA 
GGGTCTGTGG TGCATTTCTG CGAGTCCACA TCTTCAACGC AGAAGGGCGA GTCTCTGGTG 
GACTCTGTGA ACACCATGAG CTGCTATGCT GATGTCATTG TTCTGCGGCA CCCCATACCA 
GGAGCTGTAG AGAGTGCGGC AAGACATTGT CGGCGTCCGG TGATCAATGC TGGTGATGGG 
GTCGGAGAAC ATCCAACTCA GGCCCTTCTG GACATCTTTA CAATCCGAGA AGAGCTGGGA 
ACGGTCAATG GCATGACTAT TACCATGGTA GGCGACCTGA AACATGGGCG AACTGTGCAT 
TCTCTAGCTA GACTTCTCAC TCAGTACAGA ATCACTTTAC GCTATGTGGC TCCAAAGAAT 
CTGAGCATGC CTGCAGAAAT CATCGACTTT GTGGCCTCCA AGGGAATTAA ACAGGAGGAG 
TTTAATAGCA TTGAAGAAGC CCTTCCTGAC ACTGACGTTC TGTACATGAC GAGAATACAG 
AAAGAGCGGT TCTCTTCTGA AAAAGAGTAC AATGCGTGTT TTGGCCAGTT CATCCTAACC 
CCACATATCA TGACGGGAGC CAAAAGAAAG ATGGTGGTCA TGCATCCTCT TCCCAGAGTC 
AACGAGATCA GTGTGGAAGT GGACACAGAT CCTCGGGCTG CTTACTTCCG GCAGGCAGAG 
AACGGGATGT ATATTCGGAT GGCACTACTG GCTACTGTAT TGGGGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293160.1
CDS	627..7397
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPGFKRSTMHPQKAFAEEEVSLRAPFDAELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	XM_009294885.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294885.3

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGCC 
GAAGAGGAAG TGTCTTTAAG AGCACCATTT GATGCAGAAT TGGCTCTACC AGCAGAGGCA 
TACGCTCACC CTCCTCCCCT GGCCAGGATC CTGTCACCTC AAGCTGGGCA ACCGCAGATC 
CTGCCTCACC CTCAAACCTC CCCGCTGCTG CACCCGCTGG TGGGCCAGCA CATCCTGTCT 
GTGCGCCAGT TCAGCAAGGA ACAGATGTCA CATCTGTTCA ATGTGGCACA TACTCTTCGT 
CTCATGGTGC AGAAGGAACG ACCGTTGGAT ATTTTGAAGG GTAAAGTCAT GGCCTCTATG 
TTTTACGAGG TGAGCACTCG CACCAGCAGT TCATTCGCAG CAGCCATGCA TCGTCTGGGA 
GGGTCTGTGG TGCATTTCTG CGAGTCCACA TCTTCAACGC AGAAGGGCGA GTCTCTGGTG 
GACTCTGTGA ACACCATGAG CTGCTATGCT GATGTCATTG TTCTGCGGCA CCCCATACCA 
GGAGCTGTAG AGAGTGCGGC AAGACATTGT CGGCGTCCGG TGATCAATGC TGGTGATGGG 
GTCGGAGAAC ATCCAACTCA GGCCCTTCTG GACATCTTTA CAATCCGAGA AGAGCTGGGA 
ACGGTCAATG GCATGACTAT TACCATGGTA GGCGACCTGA AACATGGGCG AACTGTGCAT 
TCTCTAGCTA GACTTCTCAC TCAGTACAGA ATCACTTTAC GCTATGTGGC TCCAAAGAAT 
CTGAGCATGC CTGCAGAAAT CATCGACTTT GTGGCCTCCA AGGGAATTAA ACAGGAGGAG 
TTTAATAGCA TTGAAGAAGC CCTTCCTGAC ACTGACGTTC TGTACATGAC GAGAATACAG 
AAAGAGCGGT TCTCTTCTGA AAAAGAGTAC AATGCGTGTT TTGGCCAGTT CATCCTAACC 
CCACATATCA TGACGGGAGC CAAAAGAAAG ATGGTGGTCA TGCATCCTCT TCCCAGAGTC 
AACGAGATCA GTGTGGAAGT GGACACAGAT CCTCGGGCTG CTTACTTCCG GCAGGCAGAG 
AACGGGATGT ATATTCGGAT GGCACTACTG GCTACTGTAT TGGGGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_009294885.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293160.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.

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CloneID	ODa39598
Clone ID Related Accession (Same CDS sequence)	XM_009294885.3 , XM_009294885.2
Accession Version	XM_009294885.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	CAD protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGCC 
GAAGAGGAAG TGTCTTTAAG AGCACCATTT GATGCAGAAT TGGCTCTACC AGCAGAGGCA 
TACGCTCACC CTCCTCCCCT GGCCAGGATC CTGTCACCTC AAGCTGGGCA ACCGCAGATC 
CTGCCTCACC CTCAAACCTC CCCGCTGCTG CACCCGCTGG TGGGCCAGCA CATCCTGTCT 
GTGCGCCAGT TCAGCAAGGA ACAGATGTCA CATCTGTTCA ATGTGGCACA TACTCTTCGT 
CTCATGGTGC AGAAGGAACG ACCGTTGGAT ATTTTGAAGG GTAAAGTCAT GGCCTCTATG 
TTTTACGAGG TGAGCACTCG CACCAGCAGT TCATTCGCAG CAGCCATGCA TCGTCTGGGA 
GGGTCTGTGG TGCATTTCTG CGAGTCCACA TCTTCAACGC AGAAGGGCGA GTCTCTGGTG 
GACTCTGTGA ACACCATGAG CTGCTATGCT GATGTCATTG TTCTGCGGCA CCCCATACCA 
GGAGCTGTAG AGAGTGCGGC AAGACATTGT CGGCGTCCGG TGATCAATGC TGGTGATGGG 
GTCGGAGAAC ATCCAACTCA GGCCCTTCTG GACATCTTTA CAATCCGAGA AGAGCTGGGA 
ACGGTCAATG GCATGACTAT TACCATGGTA GGCGACCTGA AACATGGGCG AACTGTGCAT 
TCTCTAGCTA GACTTCTCAC TCAGTACAGA ATCACTTTAC GCTATGTGGC TCCAAAGAAT 
CTGAGCATGC CTGCAGAAAT CATCGACTTT GTGGCCTCCA AGGGAATTAA ACAGGAGGAG 
TTTAATAGCA TTGAAGAAGC CCTTCCTGAC ACTGACGTTC TGTACATGAC GAGAATACAG 
AAAGAGCGGT TCTCTTCTGA AAAAGAGTAC AATGCGTGTT TTGGCCAGTT CATCCTAACC 
CCACATATCA TGACGGGAGC CAAAAGAAAG ATGGTGGTCA TGCATCCTCT TCCCAGAGTC 
AACGAGATCA GTGTGGAAGT GGACACAGAT CCTCGGGCTG CTTACTTCCG GCAGGCAGAG 
AACGGGATGT ATATTCGGAT GGCACTACTG GCTACTGTAT TGGGGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293160.1
CDS	638..7408
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPGFKRSTMHPQKAFAEEEVSLRAPFDAELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	XM_009294885.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294885.2

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGG TTTCAAAAGA TCTACCATGC ATCCCCAAAA GGCGTTTGCC 
GAAGAGGAAG TGTCTTTAAG AGCACCATTT GATGCAGAAT TGGCTCTACC AGCAGAGGCA 
TACGCTCACC CTCCTCCCCT GGCCAGGATC CTGTCACCTC AAGCTGGGCA ACCGCAGATC 
CTGCCTCACC CTCAAACCTC CCCGCTGCTG CACCCGCTGG TGGGCCAGCA CATCCTGTCT 
GTGCGCCAGT TCAGCAAGGA ACAGATGTCA CATCTGTTCA ATGTGGCACA TACTCTTCGT 
CTCATGGTGC AGAAGGAACG ACCGTTGGAT ATTTTGAAGG GTAAAGTCAT GGCCTCTATG 
TTTTACGAGG TGAGCACTCG CACCAGCAGT TCATTCGCAG CAGCCATGCA TCGTCTGGGA 
GGGTCTGTGG TGCATTTCTG CGAGTCCACA TCTTCAACGC AGAAGGGCGA GTCTCTGGTG 
GACTCTGTGA ACACCATGAG CTGCTATGCT GATGTCATTG TTCTGCGGCA CCCCATACCA 
GGAGCTGTAG AGAGTGCGGC AAGACATTGT CGGCGTCCGG TGATCAATGC TGGTGATGGG 
GTCGGAGAAC ATCCAACTCA GGCCCTTCTG GACATCTTTA CAATCCGAGA AGAGCTGGGA 
ACGGTCAATG GCATGACTAT TACCATGGTA GGCGACCTGA AACATGGGCG AACTGTGCAT 
TCTCTAGCTA GACTTCTCAC TCAGTACAGA ATCACTTTAC GCTATGTGGC TCCAAAGAAT 
CTGAGCATGC CTGCAGAAAT CATCGACTTT GTGGCCTCCA AGGGAATTAA ACAGGAGGAG 
TTTAATAGCA TTGAAGAAGC CCTTCCTGAC ACTGACGTTC TGTACATGAC GAGAATACAG 
AAAGAGCGGT TCTCTTCTGA AAAAGAGTAC AATGCGTGTT TTGGCCAGTT CATCCTAACC 
CCACATATCA TGACGGGAGC CAAAAGAAAG ATGGTGGTCA TGCATCCTCT TCCCAGAGTC 
AACGAGATCA GTGTGGAAGT GGACACAGAT CCTCGGGCTG CTTACTTCCG GCAGGCAGAG 
AACGGGATGT ATATTCGGAT GGCACTACTG GCTACTGTAT TGGGGCGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_009294886.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293161.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.

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CloneID	ODa39599
Clone ID Related Accession (Same CDS sequence)	XM_009294886.2 , XM_009294886.3
Accession Version	XM_009294886.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	CAD protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGC CGAAGAGGAA GTGTCTTTAA GAGCACCATT TGATGCAGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293161.1
CDS	637..7368
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPAEEEVSLRAPFDAELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	XM_009294886.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294886.2

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGC CGAAGAGGAA GTGTCTTTAA GAGCACCATT TGATGCAGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_009294886.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009293161.1 Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.

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CloneID	ODa39599
Clone ID Related Accession (Same CDS sequence)	XM_009294886.2 , XM_009294886.3
Accession Version	XM_009294886.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGC CGAAGAGGAA GTGTCTTTAA GAGCACCATT TGATGCAGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293161.1
CDS	627..7358
Translation	MSLKMASLVLEDGTTFKGRLFGAVSSVSGEVVFQTGMVGYPEALTDPSYKSQILTLTYPMIGNYGIPKDEDGEFGLSKWFESSQIHAAALIVGEVSENPSHWSAAKSLDEWLREQGIPGLQGVDTRCLTKKIREKGTMLGKLVVEGTPADNIPFDNPDARNLVKEVSMKAPKVFNPEGTVRITAIDCGIKYNQIRCLCQRGARVTVVPWDQPLDSNDFDGLFISNGPGNPEYCKETVENIRKVACVENPKPIFGICLGHQLLSLVIGAKTYKMKYGNRGHNQPCIHKGTSRCYITSQNHGFAVDPETLPKDWDVLFTNANDQTSEGIVHNHKPLFSVQFHPEHMAGPTDLVGLFDVFLDTVRDVKENKAGKSVKQRLMEHLTFPGSPDPNAFVRPRKVLVLGSGGLSIGQAGEFDYSGSQAIKAMKEENIQTILINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGVLLTFGGQTALNCGVELKKQGVLEKYKVRVLGTPVASIEMTEDRKIFVEKMEEINEHVAPSEAAMSVEQAVAAAERLGYPVLVRSAFALGGLGSGFANNRDEMITLVTQAFAHTSQVLVDKSLKGWKEIEYEVVRDAYDNCVTVCNMENIDPLGIHTGESIVVAPSQTLNDYEYNMLRNTAIKVIRHLGVVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLSLGIPLPVLKNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMAIGRSFEEAFQKALRMVDENCVGFDHTIKPESEEELQTPTDKRIFVLAAALHAGYTVERLYELTKIDHWFLHKMKNIADHKKLLETYKQDESAMPPEIMRKAKQLGFSDKQIAQAVQSTELAVRKLRRDWKIFPVVKQIDTVAAEWPAQTNYLYLTYHGSESDVVFEQPHVMVIGSGVYRIGSSVEFDWCAVGCIMELRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYEMENPEGVILSMGGQLPNNIAMSLHRQQCRILGTSPEFIDNAENRFKFSRMLDTIGISQPRWKELSDTESAVNFCETVGYPCLVRPSYVLSGAAMNVAYSDTDMEKFLSSAVAVSKEHPVVISKFIQEAKEIDVDAVACDGEVIAIAVSEHVENAGVHSGDATLVTPPQDINQKTMERIKMIVHAIGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPYVSKTLGVDLVALATRVILGEEVEAVGLMRGNGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPKKNILLSIGSYKNKSELLPTVQTLESLGYNLYASLGTADFYTEHGVKVMAVDWPFEEESDCPNKDKQRNIMDYLEENHFDLVINLSMRNSGGRRLSSFVTKGYRTRRMAIDFSVPLITDIKCTKLFVQALKQIGQAPRVKTHVDSMTSQKLIRLPGLIDVHVHLREPGATHKEDFSSGTAAALAGGITMVCAMPNTNPAIIDPNSFTMAQKLAKAGCRCDYALFVGASSDNAALLPSIASSTAGLKMYLNDTYSTLKMDNVSLWMEHFEKWPKHLPIVAHAEKQTVAAILLVAQLYQRPVHICHVAKKEEILIIRAAKQKGIQVTCEVAPHHLFLCEDNVPVIGKDKAQVRPMLGTREDMEALWENLDIIDCFATDHAPHSAEEKISEKPPPGYPGLETMLPLLFTAVSEGRLTVDDIIKRLYENPRKIFSLPAQEDTYVEVDLEQEWIIPKHMQFTKSKWTPFEGMKVKGKVMRVVLRGEVAYIDGQVLVPPGYGQDVKSWSATPTATTEVIMDAPKRVIEAMTPERPRQAAPVDVVRSRAPSPRRSAGDGRFILPPRIHRSSDPGLPPAEEEVSLRAPFDAELALPAEAYAHPPPLARILSPQAGQPQILPHPQTSPLLHPLVGQHILSVRQFSKEQMSHLFNVAHTLRLMVQKERPLDILKGKVMASMFYEVSTRTSSSFAAAMHRLGGSVVHFCESTSSTQKGESLVDSVNTMSCYADVIVLRHPIPGAVESAARHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLARLLTQYRITLRYVAPKNLSMPAEIIDFVASKGIKQEEFNSIEEALPDTDVLYMTRIQKERFSSEKEYNACFGQFILTPHIMTGAKRKMVVMHPLPRVNEISVEVDTDPRAAYFRQAENGMYIRMALLATVLGR

Target ORF information:

RefSeq Version	XM_009294886.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294886.3

ORF Insert Sequence:

ATGTCGCTGA AAATGGCATC TTTAGTGCTG GAGGACGGGA CGACATTTAA AGGCCGTCTG 
TTCGGTGCCG TATCTTCAGT GTCTGGTGAA GTTGTTTTCC AGACAGGAAT GGTGGGTTAT 
CCGGAGGCAC TCACAGATCC GTCTTACAAG TCCCAAATAC TAACTCTGAC GTACCCGATG 
ATTGGCAATT ATGGTATTCC AAAAGATGAA GATGGAGAGT TTGGACTCAG CAAGTGGTTT 
GAATCTTCCC AAATCCATGC TGCTGCTCTC ATTGTTGGGG AGGTTTCGGA GAATCCCAGT 
CACTGGAGCG CCGCCAAATC TCTGGATGAG TGGCTCAGAG AGCAAGGCAT CCCTGGCCTC 
CAAGGTGTTG ACACTCGATG CTTGACCAAA AAGATCAGAG AGAAGGGAAC CATGTTGGGA 
AAACTTGTGG TGGAAGGAAC TCCAGCCGAC AATATTCCGT TTGACAATCC AGATGCCCGA 
AACCTTGTTA AAGAAGTTTC CATGAAAGCA CCAAAAGTGT TTAATCCAGA GGGCACAGTG 
AGAATCACTG CTATAGACTG TGGCATCAAG TACAACCAGA TACGTTGCTT GTGCCAGAGA 
GGCGCCCGGG TCACTGTTGT CCCCTGGGAT CAACCTCTGG ACAGCAATGA CTTTGATGGG 
TTGTTTATCA GCAACGGTCC TGGCAATCCT GAGTACTGTA AGGAGACTGT AGAGAATATA 
CGCAAAGTGG CATGTGTGGA GAATCCCAAA CCTATCTTTG GAATTTGCTT GGGCCATCAG 
CTGCTGTCTT TGGTCATTGG CGCAAAGACA TACAAAATGA AGTACGGTAA TCGTGGCCAT 
AATCAGCCAT GCATCCATAA AGGAACCAGT CGCTGTTACA TCACCTCTCA GAATCACGGG 
TTTGCTGTGG ATCCAGAAAC TCTTCCAAAG GACTGGGATG TGCTCTTCAC CAATGCTAAT 
GACCAAACCA GCGAGGGCAT CGTTCATAAT CACAAACCAC TCTTCAGTGT CCAGTTCCAT 
CCTGAACACA TGGCAGGTCC CACAGACCTT GTTGGTCTCT TTGATGTGTT TCTGGATACA 
GTCAGAGATG TAAAGGAGAA CAAGGCTGGA AAATCAGTGA AGCAAAGATT AATGGAGCAC 
CTCACCTTTC CTGGATCTCC TGACCCAAAT GCCTTTGTTC GACCACGTAA AGTTCTGGTT 
CTGGGTTCAG GTGGTCTTTC CATTGGACAG GCTGGAGAGT TTGACTATTC TGGATCTCAG 
GCTATAAAAG CTATGAAAGA AGAGAATATC CAAACCATTC TCATCAACCC AAACATTGCC 
ACAGTACAAA CCTCAAAGGG CCTGGCAGAC AAAGTCTACT TCCTTCCGAT CACACCAGAG 
TACGTCACAC AGGTGATAAA GAACGAGCGT CCAGATGGGG TGTTGCTCAC TTTCGGAGGT 
CAGACAGCCC TGAACTGTGG TGTGGAACTC AAAAAACAAG GAGTGCTGGA AAAATACAAA 
GTGCGTGTGC TTGGTACCCC AGTGGCCTCC ATAGAGATGA CAGAAGACAG GAAGATCTTT 
GTGGAGAAGA TGGAGGAAAT CAACGAGCAT GTCGCCCCCA GTGAGGCGGC CATGTCTGTT 
GAACAGGCAG TGGCTGCAGC TGAAAGACTT GGTTATCCGG TTCTGGTCCG TTCTGCGTTT 
GCCTTGGGTG GACTGGGTTC AGGCTTTGCC AATAACAGAG ATGAGATGAT CACGTTGGTC 
ACTCAGGCCT TTGCCCACAC ATCACAAGTC CTGGTTGACA AATCGCTTAA AGGCTGGAAG 
GAAATTGAGT ATGAAGTAGT GCGGGATGCC TATGACAATT GTGTTACGGT GTGTAACATG 
GAGAACATCG ATCCTCTAGG AATTCACACT GGAGAGTCCA TAGTGGTAGC TCCTAGTCAG 
ACACTAAACG ACTATGAATA CAACATGCTG AGAAACACAG CCATCAAAGT TATTCGCCAC 
TTGGGTGTTG TGGGAGAGTG CAACATTCAG TATGCCCTGA ATCCTGAATC AGAGCAGTAC 
TACATCATTG AAGTCAATGC CCGTCTCTCT CGAAGCTCTG CATTGGCCAG TAAGGCCACA 
GGTTACCCGC TGGCCTACGT GGCTGCTAAA CTCAGCTTGG GAATCCCTCT GCCCGTGCTC 
AAAAATTCAG TGACTAATTC CACAACCGCC AACTTTGAGC CCAGTCTGGA TTACTGCGTG 
GTGAAGGTTC CTCGCTGGGA TTTAAGCAAG TTCCTCAGAG TCAGCACTAA AATAGGCAGC 
TCCATGAAGA GTGTTGGAGA AGTGATGGCC ATCGGCCGCA GCTTTGAAGA GGCCTTCCAG 
AAAGCTTTGA GAATGGTAGA TGAGAACTGC GTTGGATTCG ATCACACCAT CAAGCCAGAG 
TCGGAAGAGG AGTTGCAGAC TCCCACAGAC AAGCGCATCT TTGTTCTGGC AGCTGCTCTG 
CATGCTGGAT ATACAGTAGA GCGGCTCTAT GAGCTGACCA AGATAGACCA CTGGTTTCTG 
CATAAGATGA AGAACATCGC AGACCACAAG AAGCTTCTGG AGACGTATAA GCAGGATGAG 
AGTGCAATGC CTCCAGAAAT CATGAGGAAA GCCAAGCAAC TGGGCTTCTC TGACAAACAG 
ATCGCTCAAG CTGTGCAAAG CACTGAACTG GCTGTGAGGA AGTTGAGACG GGACTGGAAG 
ATCTTTCCAG TGGTCAAACA GATCGACACT GTGGCTGCAG AGTGGCCCGC TCAAACCAAC 
TACCTGTACC TGACATATCA CGGCTCAGAA AGTGACGTAG TGTTCGAGCA ACCACATGTC 
ATGGTGATTG GCTCCGGGGT TTACCGCATT GGCAGCAGTG TGGAGTTTGA CTGGTGTGCT 
GTTGGGTGTA TTATGGAACT CAGAAAGATG GGATATAAAA CCATTATGGT GAACTACAAC 
CCGGAAACAG TCAGCACTGA TTATGATATG TGTGATAGAC TGTATTTTGA TGAGATCTCC 
TTTGAGGTGG TGATGGATAT CTATGAGATG GAGAATCCAG AGGGTGTTAT TCTGTCTATG 
GGTGGGCAGC TGCCCAATAA CATCGCCATG TCCCTTCACA GACAGCAGTG CAGGATCCTG 
GGCACCTCAC CTGAGTTCAT AGACAATGCG GAAAACCGCT TCAAATTCTC CAGAATGCTG 
GACACCATCG GGATCAGCCA GCCTCGGTGG AAAGAGCTCT CTGATACTGA GTCTGCTGTG 
AATTTCTGTG AGACGGTGGG TTATCCCTGC TTGGTGAGGC CATCATATGT TCTGAGCGGA 
GCTGCGATGA ACGTGGCCTA CTCAGACACG GATATGGAGA AGTTTCTGAG CAGTGCTGTT 
GCTGTGTCGA AGGAACACCC TGTCGTTATT TCCAAATTCA TTCAGGAGGC CAAGGAGATT 
GATGTGGACG CCGTGGCCTG TGATGGAGAG GTTATTGCCA TCGCGGTTTC TGAGCATGTG 
GAGAACGCAG GGGTTCATTC TGGAGACGCC ACACTGGTGA CTCCTCCACA GGACATTAAC 
CAGAAAACCA TGGAGCGCAT TAAGATGATC GTTCATGCTA TTGGGCAGGA GCTTCAGGTC 
ACTGGCCCGT TCAACCTGCA GCTCATTGCC AAGGATGATC AGCTGAAGGT GATCGAGTGC 
AATGTGCGAG TCTCGCGCTC CTTCCCTTAT GTGTCCAAAA CACTTGGTGT GGATCTTGTC 
GCGCTTGCAA CAAGAGTCAT CTTGGGAGAG GAAGTGGAAG CTGTTGGGCT GATGAGGGGG 
AATGGCATTG TTGGAGTTAA GGTTCCTCAG TTCTCCTTCT CCCGCCTGGC TGGAGCAGAT 
GTGGTTTTGG GTGTTGAGAT GACCAGCACT GGAGAAGTGG CCTGCTTCGG GGAGAACCGA 
TATGAGGCCT ATCTCAAAGC CATGCTTAGC ACCGGCTTCA AGATCCCTAA AAAGAACATT 
CTGCTCTCTA TTGGTAGTTA CAAGAATAAG AGTGAGCTGC TGCCCACAGT ACAGACTTTG 
GAGAGTTTGG GTTATAACTT GTATGCAAGT CTTGGCACTG CTGACTTCTA CACTGAACAT 
GGAGTCAAGG TAATGGCAGT GGACTGGCCA TTTGAAGAGG AGAGTGACTG TCCAAATAAG 
GACAAACAGC GCAACATCAT GGACTATCTG GAGGAGAACC ATTTTGACTT GGTCATCAAT 
CTGTCTATGA GGAACTCTGG AGGAAGACGC TTGTCCTCTT TTGTGACCAA AGGATACCGA 
ACTCGTCGAA TGGCTATTGA CTTCTCAGTG CCACTCATAA CTGACATAAA GTGCACTAAG 
CTCTTTGTTC AAGCACTGAA GCAAATTGGA CAGGCTCCTC GCGTGAAGAC ACATGTAGAC 
AGCATGACCT CACAGAAACT CATCCGTTTG CCAGGTTTGA TTGATGTCCA TGTACATCTG 
CGAGAGCCTG GTGCCACCCA TAAAGAAGAC TTCTCGTCGG GCACTGCTGC TGCACTCGCT 
GGAGGTATCA CTATGGTGTG TGCAATGCCC AACACTAACC CTGCCATCAT CGACCCCAAC 
TCCTTTACTA TGGCACAAAA GCTTGCAAAG GCTGGGTGCC GCTGTGATTA TGCACTTTTC 
GTGGGGGCTT CATCCGACAA CGCTGCTCTA CTTCCGTCCA TTGCAAGCTC TACAGCTGGT 
CTGAAGATGT ACTTGAATGA CACCTACTCT ACTTTGAAGA TGGACAATGT CTCGCTGTGG 
ATGGAGCACT TCGAAAAGTG GCCGAAGCAC TTGCCCATTG TGGCACATGC AGAGAAACAG 
ACAGTGGCTG CAATCCTGCT GGTGGCACAG CTCTATCAGA GACCTGTTCA TATCTGCCAC 
GTTGCCAAGA AAGAAGAGAT TCTGATCATT CGAGCTGCTA AACAAAAAGG GATCCAGGTG 
ACGTGTGAAG TGGCACCTCA CCATCTTTTC TTGTGTGAAG ATAACGTGCC CGTCATTGGA 
AAAGACAAGG CACAAGTGCG CCCCATGCTG GGCACACGCG AGGACATGGA GGCATTATGG 
GAAAATCTAG ACATCATTGA CTGTTTTGCA ACAGACCACG CTCCACATTC AGCGGAAGAG 
AAGATCTCAG AGAAGCCTCC TCCAGGTTAC CCAGGTCTGG AGACGATGCT GCCTCTGCTG 
TTCACGGCCG TCAGCGAGGG TCGCCTTACA GTCGATGACA TTATCAAGAG GCTGTATGAA 
AACCCCCGAA AAATCTTCTC CCTTCCTGCT CAAGAAGACA CCTATGTTGA GGTGGATTTG 
GAGCAGGAGT GGATCATTCC AAAGCACATG CAATTCACCA AGTCAAAGTG GACACCTTTT 
GAGGGCATGA AAGTGAAGGG AAAAGTCATG AGGGTTGTGC TCAGAGGCGA AGTGGCTTAT 
ATTGATGGAC AGGTGTTGGT ACCTCCTGGT TATGGTCAGG ATGTAAAGTC TTGGTCAGCA 
ACCCCAACAG CAACCACTGA GGTGATAATG GATGCTCCTA AGCGTGTGAT CGAGGCAATG 
ACTCCTGAGC GCCCCCGTCA GGCGGCACCT GTTGATGTGG TCCGCAGTCG TGCCCCGAGC 
CCACGCCGCT CAGCTGGAGA TGGACGGTTC ATTCTGCCAC CTCGTATCCA CCGGTCATCT 
GATCCCGGCC TGCCTCCAGC CGAAGAGGAA GTGTCTTTAA GAGCACCATT TGATGCAGAA 
TTGGCTCTAC CAGCAGAGGC ATACGCTCAC CCTCCTCCCC TGGCCAGGAT CCTGTCACCT 
CAAGCTGGGC AACCGCAGAT CCTGCCTCAC CCTCAAACCT CCCCGCTGCT GCACCCGCTG 
GTGGGCCAGC ACATCCTGTC TGTGCGCCAG TTCAGCAAGG AACAGATGTC ACATCTGTTC 
AATGTGGCAC ATACTCTTCG TCTCATGGTG CAGAAGGAAC GACCGTTGGA TATTTTGAAG 
GGTAAAGTCA TGGCCTCTAT GTTTTACGAG GTGAGCACTC GCACCAGCAG TTCATTCGCA 
GCAGCCATGC ATCGTCTGGG AGGGTCTGTG GTGCATTTCT GCGAGTCCAC ATCTTCAACG 
CAGAAGGGCG AGTCTCTGGT GGACTCTGTG AACACCATGA GCTGCTATGC TGATGTCATT 
GTTCTGCGGC ACCCCATACC AGGAGCTGTA GAGAGTGCGG CAAGACATTG TCGGCGTCCG 
GTGATCAATG CTGGTGATGG GGTCGGAGAA CATCCAACTC AGGCCCTTCT GGACATCTTT 
ACAATCCGAG AAGAGCTGGG AACGGTCAAT GGCATGACTA TTACCATGGT AGGCGACCTG 
AAACATGGGC GAACTGTGCA TTCTCTAGCT AGACTTCTCA CTCAGTACAG AATCACTTTA 
CGCTATGTGG CTCCAAAGAA TCTGAGCATG CCTGCAGAAA TCATCGACTT TGTGGCCTCC 
AAGGGAATTA AACAGGAGGA GTTTAATAGC ATTGAAGAAG CCCTTCCTGA CACTGACGTT 
CTGTACATGA CGAGAATACA GAAAGAGCGG TTCTCTTCTG AAAAAGAGTA CAATGCGTGT 
TTTGGCCAGT TCATCCTAAC CCCACATATC ATGACGGGAG CCAAAAGAAA GATGGTGGTC 
ATGCATCCTC TTCCCAGAGT CAACGAGATC AGTGTGGAAG TGGACACAGA TCCTCGGGCT 
GCTTACTTCC GGCAGGCAGA GAACGGGATG TATATTCGGA TGGCACTACT GGCTACTGTA 
TTGGGGCGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish Bone and General Physiology Are Differently Affected by Hormones or Changes in Gravity.
PloS one10(6)e0126928(2015)
Aceto J,Nourizadeh-Lillabadi R,Mar?e R,Dardenne N,Jeanray N,Wehenkel L,Alestr?m P,van Loon JJ,Muller M

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad) regulates Notch signaling and vascular development in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists244(1)1-9(2015 Jan)
Coxam B,Neyt C,Grassini DR,Le Guen L,Smith KA,Schulte-Merker S,Hogan BM

The role of the DNA damage response in zebrafish and cellular models of Diamond Blackfan anemia.
Disease models & mechanisms7(7)895-905(2014 Jul)
Danilova N,Bibikova E,Covey TM,Nathanson D,Dimitrova E,Konto Y,Lindgren A,Glader B,Radu CG,Sakamoto KM,Lin S

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CAD cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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