cad cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	cad
Entrez Gene ID	100125119
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms	xcad
General protein information	Preferred Names carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Names CAD protein
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004920201.2	XP_004920258.2	CAD protein
XM_031901931.1	XP_031757791.1	CAD protein isoform X2
XM_031901932.1	XP_031757792.1	CAD protein isoform X3
XM_031901930.1	XP_031757790.1	CAD protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa58217	XM_031901932.1 Latest version!	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa58215	XM_031901930.1 Latest version!	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa58216	XM_031901931.1 Latest version!	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OXa30698	XM_004920201.2 Latest version!	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cad ( XM_031901932.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757792.1 Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa58217
Clone ID Related Accession (Same CDS sequence)	XM_031901932.1
Accession Version	XM_031901932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	CAD protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG GTACTGCGGT GCTACTGCTG GAGGATGGAA CCCGGGTATC CGGAACCGTG 
TTCGGGGCGG TGCGTCCGGT GTCCGGGGAA GTGGTGTTCC AGACGGGCAT GGTGGGCTAC 
CCCGAGGCCC TCACCGACCC CTCCTACAGG GCCCAGATCC TGGTACTGAC ATATCCGCTG 
ATTGGGAACT ACGGGATCCC GCAGGATGAA CTGGACCAGT TTGGCCTCAG TCGGTGGTTC 
GAGTCCAGCA AGATTCACGC CTCGGCGCTG ATTATTGGGG AGCGGTCCCG GAACCCCAGT 
CACTGGAGCT GTGACATCTC ATTGAACCAG TGGCTCAAAC AGCAGGGAAT CCCCGGACTG 
GAAGGTGTGG ATACCCGGGC ACTCACCAAG AAGATCCGAG AGAAGGGCAC CCTGCTGGGC 
AAGCTCATAC CAGTGGGCAC AGAGGAGAGC CAGGTCCCTT ATGAAGATCC TGCCAAGAGG 
AACCTGGTTC AGGAGGTGTC ATTGAAGGAG CCCCGAGTGT ACAATGCGGG GGGCACAGTG 
AGAATCGCTG CCCTGGACTG TGGTTTGAAG TTCAACCAGA TTCGCTGCCT GTGCCAGAGG 
GGGGCCGAGG TGACGGTGCT GCCATGGGAC TATCCAATCA GCAGTGCAGA GTGTGACGGC 
CTGTTCCTCA GTAACGGCCC CGGGGACCCC ACGTTCTGTC AGAGCTCCGT GCGGAACATC 
AGGGACTTCA TTTCCGAGGA GAAGCCGAAA CCCGTGTTTG GGATCTGTCT GGGCCACCAG 
ATCCTCTCTC TGGTTATAGG CGCCCACACC TACAAGATGA AGTACGGGAA TCGTGGGCAC 
AACCAGCCGT GCATCCATGA GAGCTCTCGG CGCTGCTTCA TAACCTCACA GAACCATGGA 
TACGCCGTGG ACCCAAACAC TTTACCGGAA GGCTGGTCTC CTCTGTTCAC GAATGCCAAC 
GACCAATCAA ATGAGGGAAT TATCCATGAT TCTAAGCCCT TTTTCAGTGT GCAGTTCCAC 
CCCGAGCACT GTGCTGGGCC CATGGATCTG GTCGGCCTGT TTGATGTGTT CCTGGACACT 
GTGAGAGACC GGAAGGCTGG AGATTCAGGA GAGAAGACGG TTAAACAGCG AGTGACTGAG 
CTGCTCACAT ACAAGGAGTC TCCCAGTGTC ACAGACCTAG AGGCCTCTCG CCCACACAAA 
GTGCTGATCC TGGGCTCAGG GGGGCTGTCG ATTGGCCAGG CCGGGGAGTT CGACTACTCT 
GGCTCCCAGG CCATCAAAGC TCTGAAGGAA GAGAACATCC AGACTGTCCT GATTAACCCC 
AACATCGCCA CAGTGCAGAC ATCGAAAGGC CTCGCCGACA AGGTTTATTT CCTGCCAATC 
ACCCCTGAAT ATGTCACTCA GGTGATAAAG AATGAGCGGC CAGATGGGAT CCTCCTGACG 
TTTGGGGGTC AGACTGCATT GAACTGTGGG GTGGAGCTGA CCAAGCAGGG AGTCCTAGAG 
AAGTACAATG TGCGGGTTCT GGGGACCCCT GTGAAGTCCA TTGAAATGAC GGAAGATCGC 
AAAGTGTTTG TGGAGAAGAT GGAGGAGATT GGAGAACACG TGGCCCCCAG TGAGGCAGCT 
TTCTCCTTAG AGCAGGCCCA GGCTGCTGCA GAACGTCTCG GGTATCCGGT TTTGGTCCGG 
TCGGCCTACG CGCTGGGCGG CCTTGGATCT GGATTTGCCA GTACCAAGGA GGAGTTGACT 
GCCCTGGTGT GCCAGGCCTT CGCCCACACC ACACAGGTCC TGGTGGATAA ATCCCTGAAG 
GGCTGGAAGG AGATAGAGTA CGAAGTCGTG CGGGACGCCT ATAATAACTG TGTCACGGTG 
TGCAACATGG AGAACGTAGA TCCCCTGGGC ATTCACACTG GCGAGTCCAT CGTGGTAGCT 
CCCAGCCAGA CGCTGAACGA TCGAGAGTAC AACATGCTGC GCAACACGGC CATTAAGGTT 
ATCCAGCACC TCGGCATTGT GGGCGAGTGT AACATACAGT ACGCCCTAAA CCCGGAGTCT 
GAGCAGTATT ACATCATCGA GGTGAACGCA CGGCTGTCTC GCAGCTCAGC CCTGGCGAGT 
AAAGCAACTG GTTACCCCCT GGCGTATGTG GCTGCCAAGC TGGCACTGGG AATTCCTCTG 
CCCGTTCTCA GAAACTCCGT GACCAACTCC ACCACAGCCA ACTTTGAGCC CAGCCTGGAT 
TACTGTGTGG TGAAGGTTCC GCGCTGGGAC CTGAGCAAGT TCTTGCGAGT CAGCACCAAG 
ATTGGCAGCT CCATGAAGAG TGTGGGGGAG GTCATGTCTG TTGGTAGGAA CTTTGAAGAA 
GCCTTCCAGA AAGCTCTGCG CATGGTGGAC GAGAACTGCG TGGGCTTTGA TCACTGCATC 
AAATCCGCAT CTGATGAGGA ACTGGAGACG CCGACAGACA AGAGGATCTT CGTTCTGGCC 
GCTGCCCTGC GTGCAGGTTA CACTATCGAG CGCCTTTATG AGTTGACCAA GATTGACAGG 
TGGTTCCTCC ATAAGATGAA GAATATTACT GATCAGGTGG CCACGCTGGA GTCCTACCAC 
CAGGATGAGA CCTTGATGCC CCGTGAGGTC CTTCTCCGAG CCAAACAGCT GGGCTTCTCC 
GACAAGCAGA TTGCCCTGGC CGTGCAGAGC ACGGAGCTGG CAGTCCGCAA GATGCGGCAG 
GAGCTGCTGA TTTTGCCGGT AGTAAAGCAG ATTGACACGG TGGCGGCGGA GTGGCCGGCT 
CAGACCAATT ACCTGTATCT GACCTATAAT GGAACTGAGC ACGACTTGGC CTTTGAGGAG 
CCGCATGTTA TGGTGATTGG CTCTGGTGTT TACCGAATAG GCAGCAGTGT GGAGTTTGAT 
TGGTGCGCGG TGGGCTGCAT CCAGGAACTG CGTAAGATGG GATTTAAGAC CATCATGGTG 
AATTATAACC CCGAGACTGT CAGCACAGAC TATGACATGT GTGACCGCCT GTATTTTGAC 
GAGATCTCAT TTGAGGTGGT GATGGATATC TATGAGCTGG AGAACCCAGA GGGAATCATA 
CTGTCCATGG GTGGCCAGCT GCCCAATAAT ATTGCCATGT CCCTACATCG CCAGCAGTGC 
CGGGTGCTGG GCACTTCTCC TGAGGCCATC GACTCCGCTG AGAACAGGTT TAAGTTTTCT 
CGCATGTTGG ACACGATCGG AATCAGTCAG CCGCAGTGGA AAGAGCTGTC TGACATGGAG 
TCGGCCGTTC AGTTCTGTAA CACCGTCGGT TTCCCCTGCG TGGTGCGCCC ATCCTACGTG 
CTAAGTGGAG CCGCCATGAA TGTGGCCCAT TCAGATGGAG ACCTGGAGAA GGTGCTCAGT 
AACGCTGTGG CTGTGTCCAA GGAGCATCCC GTGGTCATCT CTAAATTCAT CCAGGAGGCC 
AAGGAAATTG ATGTGGATGC TGTGGCATGT GATGGAGTTG TTGTGGCCGT GGCTATTTCA 
GAACATGTGG AGAATGCTGG GGTTCACTCT GGGGATGCCA CACTGGTGAC CCCTGCTCAG 
GATATCACCC CCAAAACCTT GGAAAGGATT AAAGCCATTG TCCATGCTGT GGGGCTGGAG 
CTCCAAGTCA ACGGACCCTT TAACCTGCAG CTCATTGCAA AGGACGACCA GCTGAAAGTG 
ATCGAGTGCA ATGTCCGTGT GTCGCGCTCC TTTCCCTTTG TATCGAAGAC TCTGGGAGTC 
AATCTAGTCG CTCTGGCAAC GCAAGTCATT ATGGGAGAAG AGGTTGAACC TGTTGGTGTG 
ATGACTGGTA CTGGCATTGT AGGGGTTAAG GTCCCACAGT TCTCGTTCTC ACGCTTGGCC 
GGCGCGGACG TGGTCCTTGG GGTGGAGATG ACCAGCACCG GGGAGGTTGC CTGCTTTGGA 
GAGAACCGAT ACGAGGCCTA TCTAAAAGCC ATGCTAAGTA CCGGCTTCAA AATTCCACAG 
AAGAACATCC TACTGACCAT CGGGAGCTAC AAGAACAAGA GTGAGCTGCT GCCCACGGTG 
CGAGCCATGG AGAGTCTTGG CTACAGTCTG TATGCCAGCC TGGGAACTGC TGACTTCTAT 
ACGGAGCATG GCATCAAGGT AAAGGCAATG GATTGGCCCT TTGAGGAGAG TGAGAATGAC 
GGGCCCCTAC GGGAGAAGCA GGGCAGCATC ATGGACCATC TGTCAGAGAA TCACTTTGAT 
TTGGTCATTA ATTTGTCCAT GCGGAATTCT GGGGGGCGCC GACTCTCCTC CTTCGTTACC 
CGTGGGTACC GTGCCCGCCG CTTGGCTGTG GACTATTCTG TGCCCCTCAT CATTGATATC 
AAGTGCACCA AACTCTTTGT GGAGGCTCTG CGTCAGATTG GGGGGGCGCC CCCTGTGAAG 
ACTCATGTCG ACTGTATGAC CTCACAGAAA CTTATCAGGC TGCCAGGCCT AGTGGACATC 
CACGTGCACC TGCGGGAACC TGGCGCTACT CATAAGGAGG ACTTTTCTTC TGGCACAGCA 
GCTGCCCTGG CTGGTGGAAT TACCCTTGTA TGTGCCATGC CCAACACCAG CCCAGCCATC 
ACTGATGCCA GTTCCTTCTC TCTGGCACAG AAGCTGGCAG AGGCCGGTGC CCGCTGTGAC 
TTTGCCCTCT TCCTCGGTGC TTCCTCAGAG AATGCCGCTT CTCTTTCTTC GATGGCCAGT 
TCAGCCGCCG GCCTTAAGAT GTATCTGAAC GACACCTATT CGCAGCTTAA GATGGACAAT 
GTTTCTGTGT GGATGGAGCA CTTTGAGAAG TGGCCGGCAC ACATGCCCAT TGTGGCCCAT 
GCAGAGAAAC AGACCGTGGC GGCAATTCTC ATGGTGGCTC AGCTGTACCA GCGCCCTGTG 
CACATTTGCC ACGTTGCAAA AAAAGAGGAG ATCCTTATTA TCAAAGCAGC CAAGCAGAAG 
GGAATCCAAG TTACATGCGA GGTGGCCCCT CATCACCTTT TCCTGTGTCT CGATGATCTG 
AAGAAAATCG GGGAGACACG GGGGCAAGTA CGTCCCATGT TGGGGACTCG GGAGGATCTA 
GAATCTCTGT GGGAGAACTT GGATATAATA GACTGTTTTG CCACTGACCA CGCCCCTCAC 
TCTGTGGAGG AGAAGGACGG TCCAAACCCA CCTCCAGGGT ATCCCGGCTT GGAGACCATG 
CTGCCTCTGC TACTCACAGC AGTCAGTGAG GGACGTCTGA CTATTGATGA TATCATTAAG 
CGCCTGTACG ACAACCCCCA GAAGATCTTC TCTTTGCCTG TGCAGGAGAA CACCTATGTC 
GAGGTGGATT TGGAGCAAGA GTGGGTGATC CCGTCACACA TGCAGTTTAC CAAGTCCAGA 
TGGAGCCCCT TTGCTGGAAT GAAGGTGAAA GGAGCAGTGC GGCGAGTTGT TCTCCGTGGG 
GAGGTGGCCT ACATTGATGG GCAGGTACTG GTACCCATGG GATATGGACA GGATGTCAGG 
AAATGGACCC CACCTGTTGT TGCCCAATCT CACCCAGTCG CCATTAAAGA GATCCCTAAG 
ACCCCAGAGC GACATCGCCA TGTTACGGTA CAAAGTGAGA CGATTCGCAG TCGAGCACCA 
AGCCCCCGCA GACCCTGTCA GTCCGGAGAC GGGCGCTTCC ACTTGCCTCC TCGAGTGCAT 
CGTGCATCAG ATCCTGGCTT CCCAGGTGAA GCAGAACTTG GCTTACTTGG GGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757792.1
CDS	114..5810
Translation	MAAGTAVLLLEDGTRVSGTVFGAVRPVSGEVVFQTGMVGYPEALTDPSYRAQILVLTYPLIGNYGIPQDELDQFGLSRWFESSKIHASALIIGERSRNPSHWSCDISLNQWLKQQGIPGLEGVDTRALTKKIREKGTLLGKLIPVGTEESQVPYEDPAKRNLVQEVSLKEPRVYNAGGTVRIAALDCGLKFNQIRCLCQRGAEVTVLPWDYPISSAECDGLFLSNGPGDPTFCQSSVRNIRDFISEEKPKPVFGICLGHQILSLVIGAHTYKMKYGNRGHNQPCIHESSRRCFITSQNHGYAVDPNTLPEGWSPLFTNANDQSNEGIIHDSKPFFSVQFHPEHCAGPMDLVGLFDVFLDTVRDRKAGDSGEKTVKQRVTELLTYKESPSVTDLEASRPHKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTVLINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGILLTFGGQTALNCGVELTKQGVLEKYNVRVLGTPVKSIEMTEDRKVFVEKMEEIGEHVAPSEAAFSLEQAQAAAERLGYPVLVRSAYALGGLGSGFASTKEELTALVCQAFAHTTQVLVDKSLKGWKEIEYEVVRDAYNNCVTVCNMENVDPLGIHTGESIVVAPSQTLNDREYNMLRNTAIKVIQHLGIVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPVLRNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMSVGRNFEEAFQKALRMVDENCVGFDHCIKSASDEELETPTDKRIFVLAAALRAGYTIERLYELTKIDRWFLHKMKNITDQVATLESYHQDETLMPREVLLRAKQLGFSDKQIALAVQSTELAVRKMRQELLILPVVKQIDTVAAEWPAQTNYLYLTYNGTEHDLAFEEPHVMVIGSGVYRIGSSVEFDWCAVGCIQELRKMGFKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGIILSMGGQLPNNIAMSLHRQQCRVLGTSPEAIDSAENRFKFSRMLDTIGISQPQWKELSDMESAVQFCNTVGFPCVVRPSYVLSGAAMNVAHSDGDLEKVLSNAVAVSKEHPVVISKFIQEAKEIDVDAVACDGVVVAVAISEHVENAGVHSGDATLVTPAQDITPKTLERIKAIVHAVGLELQVNGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVNLVALATQVIMGEEVEPVGVMTGTGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPQKNILLTIGSYKNKSELLPTVRAMESLGYSLYASLGTADFYTEHGIKVKAMDWPFEESENDGPLREKQGSIMDHLSENHFDLVINLSMRNSGGRRLSSFVTRGYRARRLAVDYSVPLIIDIKCTKLFVEALRQIGGAPPVKTHVDCMTSQKLIRLPGLVDIHVHLREPGATHKEDFSSGTAAALAGGITLVCAMPNTSPAITDASSFSLAQKLAEAGARCDFALFLGASSENAASLSSMASSAAGLKMYLNDTYSQLKMDNVSVWMEHFEKWPAHMPIVAHAEKQTVAAILMVAQLYQRPVHICHVAKKEEILIIKAAKQKGIQVTCEVAPHHLFLCLDDLKKIGETRGQVRPMLGTREDLESLWENLDIIDCFATDHAPHSVEEKDGPNPPPGYPGLETMLPLLLTAVSEGRLTIDDIIKRLYDNPQKIFSLPVQENTYVEVDLEQEWVIPSHMQFTKSRWSPFAGMKVKGAVRRVVLRGEVAYIDGQVLVPMGYGQDVRKWTPPVVAQSHPVAIKEIPKTPERHRHVTVQSETIRSRAPSPRRPCQSGDGRFHLPPRVHRASDPGFPGEAELGLLGV

Target ORF information:

RefSeq Version	XM_031901932.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901932.1

ORF Insert Sequence:

ATGGCTGCGG GTACTGCGGT GCTACTGCTG GAGGATGGAA CCCGGGTATC CGGAACCGTG 
TTCGGGGCGG TGCGTCCGGT GTCCGGGGAA GTGGTGTTCC AGACGGGCAT GGTGGGCTAC 
CCCGAGGCCC TCACCGACCC CTCCTACAGG GCCCAGATCC TGGTACTGAC ATATCCGCTG 
ATTGGGAACT ACGGGATCCC GCAGGATGAA CTGGACCAGT TTGGCCTCAG TCGGTGGTTC 
GAGTCCAGCA AGATTCACGC CTCGGCGCTG ATTATTGGGG AGCGGTCCCG GAACCCCAGT 
CACTGGAGCT GTGACATCTC ATTGAACCAG TGGCTCAAAC AGCAGGGAAT CCCCGGACTG 
GAAGGTGTGG ATACCCGGGC ACTCACCAAG AAGATCCGAG AGAAGGGCAC CCTGCTGGGC 
AAGCTCATAC CAGTGGGCAC AGAGGAGAGC CAGGTCCCTT ATGAAGATCC TGCCAAGAGG 
AACCTGGTTC AGGAGGTGTC ATTGAAGGAG CCCCGAGTGT ACAATGCGGG GGGCACAGTG 
AGAATCGCTG CCCTGGACTG TGGTTTGAAG TTCAACCAGA TTCGCTGCCT GTGCCAGAGG 
GGGGCCGAGG TGACGGTGCT GCCATGGGAC TATCCAATCA GCAGTGCAGA GTGTGACGGC 
CTGTTCCTCA GTAACGGCCC CGGGGACCCC ACGTTCTGTC AGAGCTCCGT GCGGAACATC 
AGGGACTTCA TTTCCGAGGA GAAGCCGAAA CCCGTGTTTG GGATCTGTCT GGGCCACCAG 
ATCCTCTCTC TGGTTATAGG CGCCCACACC TACAAGATGA AGTACGGGAA TCGTGGGCAC 
AACCAGCCGT GCATCCATGA GAGCTCTCGG CGCTGCTTCA TAACCTCACA GAACCATGGA 
TACGCCGTGG ACCCAAACAC TTTACCGGAA GGCTGGTCTC CTCTGTTCAC GAATGCCAAC 
GACCAATCAA ATGAGGGAAT TATCCATGAT TCTAAGCCCT TTTTCAGTGT GCAGTTCCAC 
CCCGAGCACT GTGCTGGGCC CATGGATCTG GTCGGCCTGT TTGATGTGTT CCTGGACACT 
GTGAGAGACC GGAAGGCTGG AGATTCAGGA GAGAAGACGG TTAAACAGCG AGTGACTGAG 
CTGCTCACAT ACAAGGAGTC TCCCAGTGTC ACAGACCTAG AGGCCTCTCG CCCACACAAA 
GTGCTGATCC TGGGCTCAGG GGGGCTGTCG ATTGGCCAGG CCGGGGAGTT CGACTACTCT 
GGCTCCCAGG CCATCAAAGC TCTGAAGGAA GAGAACATCC AGACTGTCCT GATTAACCCC 
AACATCGCCA CAGTGCAGAC ATCGAAAGGC CTCGCCGACA AGGTTTATTT CCTGCCAATC 
ACCCCTGAAT ATGTCACTCA GGTGATAAAG AATGAGCGGC CAGATGGGAT CCTCCTGACG 
TTTGGGGGTC AGACTGCATT GAACTGTGGG GTGGAGCTGA CCAAGCAGGG AGTCCTAGAG 
AAGTACAATG TGCGGGTTCT GGGGACCCCT GTGAAGTCCA TTGAAATGAC GGAAGATCGC 
AAAGTGTTTG TGGAGAAGAT GGAGGAGATT GGAGAACACG TGGCCCCCAG TGAGGCAGCT 
TTCTCCTTAG AGCAGGCCCA GGCTGCTGCA GAACGTCTCG GGTATCCGGT TTTGGTCCGG 
TCGGCCTACG CGCTGGGCGG CCTTGGATCT GGATTTGCCA GTACCAAGGA GGAGTTGACT 
GCCCTGGTGT GCCAGGCCTT CGCCCACACC ACACAGGTCC TGGTGGATAA ATCCCTGAAG 
GGCTGGAAGG AGATAGAGTA CGAAGTCGTG CGGGACGCCT ATAATAACTG TGTCACGGTG 
TGCAACATGG AGAACGTAGA TCCCCTGGGC ATTCACACTG GCGAGTCCAT CGTGGTAGCT 
CCCAGCCAGA CGCTGAACGA TCGAGAGTAC AACATGCTGC GCAACACGGC CATTAAGGTT 
ATCCAGCACC TCGGCATTGT GGGCGAGTGT AACATACAGT ACGCCCTAAA CCCGGAGTCT 
GAGCAGTATT ACATCATCGA GGTGAACGCA CGGCTGTCTC GCAGCTCAGC CCTGGCGAGT 
AAAGCAACTG GTTACCCCCT GGCGTATGTG GCTGCCAAGC TGGCACTGGG AATTCCTCTG 
CCCGTTCTCA GAAACTCCGT GACCAACTCC ACCACAGCCA ACTTTGAGCC CAGCCTGGAT 
TACTGTGTGG TGAAGGTTCC GCGCTGGGAC CTGAGCAAGT TCTTGCGAGT CAGCACCAAG 
ATTGGCAGCT CCATGAAGAG TGTGGGGGAG GTCATGTCTG TTGGTAGGAA CTTTGAAGAA 
GCCTTCCAGA AAGCTCTGCG CATGGTGGAC GAGAACTGCG TGGGCTTTGA TCACTGCATC 
AAATCCGCAT CTGATGAGGA ACTGGAGACG CCGACAGACA AGAGGATCTT CGTTCTGGCC 
GCTGCCCTGC GTGCAGGTTA CACTATCGAG CGCCTTTATG AGTTGACCAA GATTGACAGG 
TGGTTCCTCC ATAAGATGAA GAATATTACT GATCAGGTGG CCACGCTGGA GTCCTACCAC 
CAGGATGAGA CCTTGATGCC CCGTGAGGTC CTTCTCCGAG CCAAACAGCT GGGCTTCTCC 
GACAAGCAGA TTGCCCTGGC CGTGCAGAGC ACGGAGCTGG CAGTCCGCAA GATGCGGCAG 
GAGCTGCTGA TTTTGCCGGT AGTAAAGCAG ATTGACACGG TGGCGGCGGA GTGGCCGGCT 
CAGACCAATT ACCTGTATCT GACCTATAAT GGAACTGAGC ACGACTTGGC CTTTGAGGAG 
CCGCATGTTA TGGTGATTGG CTCTGGTGTT TACCGAATAG GCAGCAGTGT GGAGTTTGAT 
TGGTGCGCGG TGGGCTGCAT CCAGGAACTG CGTAAGATGG GATTTAAGAC CATCATGGTG 
AATTATAACC CCGAGACTGT CAGCACAGAC TATGACATGT GTGACCGCCT GTATTTTGAC 
GAGATCTCAT TTGAGGTGGT GATGGATATC TATGAGCTGG AGAACCCAGA GGGAATCATA 
CTGTCCATGG GTGGCCAGCT GCCCAATAAT ATTGCCATGT CCCTACATCG CCAGCAGTGC 
CGGGTGCTGG GCACTTCTCC TGAGGCCATC GACTCCGCTG AGAACAGGTT TAAGTTTTCT 
CGCATGTTGG ACACGATCGG AATCAGTCAG CCGCAGTGGA AAGAGCTGTC TGACATGGAG 
TCGGCCGTTC AGTTCTGTAA CACCGTCGGT TTCCCCTGCG TGGTGCGCCC ATCCTACGTG 
CTAAGTGGAG CCGCCATGAA TGTGGCCCAT TCAGATGGAG ACCTGGAGAA GGTGCTCAGT 
AACGCTGTGG CTGTGTCCAA GGAGCATCCC GTGGTCATCT CTAAATTCAT CCAGGAGGCC 
AAGGAAATTG ATGTGGATGC TGTGGCATGT GATGGAGTTG TTGTGGCCGT GGCTATTTCA 
GAACATGTGG AGAATGCTGG GGTTCACTCT GGGGATGCCA CACTGGTGAC CCCTGCTCAG 
GATATCACCC CCAAAACCTT GGAAAGGATT AAAGCCATTG TCCATGCTGT GGGGCTGGAG 
CTCCAAGTCA ACGGACCCTT TAACCTGCAG CTCATTGCAA AGGACGACCA GCTGAAAGTG 
ATCGAGTGCA ATGTCCGTGT GTCGCGCTCC TTTCCCTTTG TATCGAAGAC TCTGGGAGTC 
AATCTAGTCG CTCTGGCAAC GCAAGTCATT ATGGGAGAAG AGGTTGAACC TGTTGGTGTG 
ATGACTGGTA CTGGCATTGT AGGGGTTAAG GTCCCACAGT TCTCGTTCTC ACGCTTGGCC 
GGCGCGGACG TGGTCCTTGG GGTGGAGATG ACCAGCACCG GGGAGGTTGC CTGCTTTGGA 
GAGAACCGAT ACGAGGCCTA TCTAAAAGCC ATGCTAAGTA CCGGCTTCAA AATTCCACAG 
AAGAACATCC TACTGACCAT CGGGAGCTAC AAGAACAAGA GTGAGCTGCT GCCCACGGTG 
CGAGCCATGG AGAGTCTTGG CTACAGTCTG TATGCCAGCC TGGGAACTGC TGACTTCTAT 
ACGGAGCATG GCATCAAGGT AAAGGCAATG GATTGGCCCT TTGAGGAGAG TGAGAATGAC 
GGGCCCCTAC GGGAGAAGCA GGGCAGCATC ATGGACCATC TGTCAGAGAA TCACTTTGAT 
TTGGTCATTA ATTTGTCCAT GCGGAATTCT GGGGGGCGCC GACTCTCCTC CTTCGTTACC 
CGTGGGTACC GTGCCCGCCG CTTGGCTGTG GACTATTCTG TGCCCCTCAT CATTGATATC 
AAGTGCACCA AACTCTTTGT GGAGGCTCTG CGTCAGATTG GGGGGGCGCC CCCTGTGAAG 
ACTCATGTCG ACTGTATGAC CTCACAGAAA CTTATCAGGC TGCCAGGCCT AGTGGACATC 
CACGTGCACC TGCGGGAACC TGGCGCTACT CATAAGGAGG ACTTTTCTTC TGGCACAGCA 
GCTGCCCTGG CTGGTGGAAT TACCCTTGTA TGTGCCATGC CCAACACCAG CCCAGCCATC 
ACTGATGCCA GTTCCTTCTC TCTGGCACAG AAGCTGGCAG AGGCCGGTGC CCGCTGTGAC 
TTTGCCCTCT TCCTCGGTGC TTCCTCAGAG AATGCCGCTT CTCTTTCTTC GATGGCCAGT 
TCAGCCGCCG GCCTTAAGAT GTATCTGAAC GACACCTATT CGCAGCTTAA GATGGACAAT 
GTTTCTGTGT GGATGGAGCA CTTTGAGAAG TGGCCGGCAC ACATGCCCAT TGTGGCCCAT 
GCAGAGAAAC AGACCGTGGC GGCAATTCTC ATGGTGGCTC AGCTGTACCA GCGCCCTGTG 
CACATTTGCC ACGTTGCAAA AAAAGAGGAG ATCCTTATTA TCAAAGCAGC CAAGCAGAAG 
GGAATCCAAG TTACATGCGA GGTGGCCCCT CATCACCTTT TCCTGTGTCT CGATGATCTG 
AAGAAAATCG GGGAGACACG GGGGCAAGTA CGTCCCATGT TGGGGACTCG GGAGGATCTA 
GAATCTCTGT GGGAGAACTT GGATATAATA GACTGTTTTG CCACTGACCA CGCCCCTCAC 
TCTGTGGAGG AGAAGGACGG TCCAAACCCA CCTCCAGGGT ATCCCGGCTT GGAGACCATG 
CTGCCTCTGC TACTCACAGC AGTCAGTGAG GGACGTCTGA CTATTGATGA TATCATTAAG 
CGCCTGTACG ACAACCCCCA GAAGATCTTC TCTTTGCCTG TGCAGGAGAA CACCTATGTC 
GAGGTGGATT TGGAGCAAGA GTGGGTGATC CCGTCACACA TGCAGTTTAC CAAGTCCAGA 
TGGAGCCCCT TTGCTGGAAT GAAGGTGAAA GGAGCAGTGC GGCGAGTTGT TCTCCGTGGG 
GAGGTGGCCT ACATTGATGG GCAGGTACTG GTACCCATGG GATATGGACA GGATGTCAGG 
AAATGGACCC CACCTGTTGT TGCCCAATCT CACCCAGTCG CCATTAAAGA GATCCCTAAG 
ACCCCAGAGC GACATCGCCA TGTTACGGTA CAAAGTGAGA CGATTCGCAG TCGAGCACCA 
AGCCCCCGCA GACCCTGTCA GTCCGGAGAC GGGCGCTTCC ACTTGCCTCC TCGAGTGCAT 
CGTGCATCAG ATCCTGGCTT CCCAGGTGAA GCAGAACTTG GCTTACTTGG GGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cad ( XM_031901930.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757790.1 Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.

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CloneID	OXa58215
Clone ID Related Accession (Same CDS sequence)	XM_031901930.1
Accession Version	XM_031901930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG GTACTGCGGT GCTACTGCTG GAGGATGGAA CCCGGGTATC CGGAACCGTG 
TTCGGGGCGG TGCGTCCGGT GTCCGGGGAA GTGGTGTTCC AGACGGGCAT GGTGGGCTAC 
CCCGAGGCCC TCACCGACCC CTCCTACAGG GCCCAGATCC TGGTACTGAC ATATCCGCTG 
ATTGGGAACT ACGGGATCCC GCAGGATGAA CTGGACCAGT TTGGCCTCAG TCGGTGGTTC 
GAGTCCAGCA AGATTCACGC CTCGGCGCTG ATTATTGGGG AGCGGTCCCG GAACCCCAGT 
CACTGGAGCT GTGACATCTC ATTGAACCAG TGGCTCAAAC AGCAGGGAAT CCCCGGACTG 
GAAGGTGTGG ATACCCGGGC ACTCACCAAG AAGATCCGAG AGAAGGGCAC CCTGCTGGGC 
AAGCTCATAC CAGTGGGCAC AGAGGAGAGC CAGGTCCCTT ATGAAGATCC TGCCAAGAGG 
AACCTGGTTC AGGAGGTGTC ATTGAAGGAG CCCCGAGTGT ACAATGCGGG GGGCACAGTG 
AGAATCGCTG CCCTGGACTG TGGTTTGAAG TTCAACCAGA TTCGCTGCCT GTGCCAGAGG 
GGGGCCGAGG TGACGGTGCT GCCATGGGAC TATCCAATCA GCAGTGCAGA GTGTGACGGC 
CTGTTCCTCA GTAACGGCCC CGGGGACCCC ACGTTCTGTC AGAGCTCCGT GCGGAACATC 
AGGGACTTCA TTTCCGAGGA GAAGCCGAAA CCCGTGTTTG GGATCTGTCT GGGCCACCAG 
ATCCTCTCTC TGGTTATAGG CGCCCACACC TACAAGATGA AGTACGGGAA TCGTGGGCAC 
AACCAGCCGT GCATCCATGA GAGCTCTCGG CGCTGCTTCA TAACCTCACA GAACCATGGA 
TACGCCGTGG ACCCAAACAC TTTACCGGAA GGCTGGTCTC CTCTGTTCAC GAATGCCAAC 
GACCAATCAA ATGAGGGAAT TATCCATGAT TCTAAGCCCT TTTTCAGTGT GCAGTTCCAC 
CCCGAGCACT GTGCTGGGCC CATGGATCTG GTCGGCCTGT TTGATGTGTT CCTGGACACT 
GTGAGAGACC GGAAGGCTGG AGATTCAGGA GAGAAGACGG TTAAACAGCG AGTGACTGAG 
CTGCTCACAT ACAAGGAGTC TCCCAGTGTC ACAGACCTAG AGGCCTCTCG CCCACACAAA 
GTGCTGATCC TGGGCTCAGG GGGGCTGTCG ATTGGCCAGG CCGGGGAGTT CGACTACTCT 
GGCTCCCAGG CCATCAAAGC TCTGAAGGAA GAGAACATCC AGACTGTCCT GATTAACCCC 
AACATCGCCA CAGTGCAGAC ATCGAAAGGC CTCGCCGACA AGGTTTATTT CCTGCCAATC 
ACCCCTGAAT ATGTCACTCA GGTGATAAAG AATGAGCGGC CAGATGGGAT CCTCCTGACG 
TTTGGGGGTC AGACTGCATT GAACTGTGGG GTGGAGCTGA CCAAGCAGGG AGTCCTAGAG 
AAGTACAATG TGCGGGTTCT GGGGACCCCT GTGAAGTCCA TTGAAATGAC GGAAGATCGC 
AAAGTGTTTG TGGAGAAGAT GGAGGAGATT GGAGAACACG TGGCCCCCAG TGAGGCAGCT 
TTCTCCTTAG AGCAGGCCCA GGCTGCTGCA GAACGTCTCG GGTATCCGGT TTTGGTCCGG 
TCGGCCTACG CGCTGGGCGG CCTTGGATCT GGATTTGCCA GTACCAAGGA GGAGTTGACT 
GCCCTGGTGT GCCAGGCCTT CGCCCACACC ACACAGGTCC TGGTGGATAA ATCCCTGAAG 
GGCTGGAAGG AGATAGAGTA CGAAGTCGTG CGGGACGCCT ATAATAACTG TGTCACGGTG 
TGCAACATGG AGAACGTAGA TCCCCTGGGC ATTCACACTG GCGAGTCCAT CGTGGTAGCT 
CCCAGCCAGA CGCTGAACGA TCGAGAGTAC AACATGCTGC GCAACACGGC CATTAAGGTT 
ATCCAGCACC TCGGCATTGT GGGCGAGTGT AACATACAGT ACGCCCTAAA CCCGGAGTCT 
GAGCAGTATT ACATCATCGA GGTGAACGCA CGGCTGTCTC GCAGCTCAGC CCTGGCGAGT 
AAAGCAACTG GTTACCCCCT GGCGTATGTG GCTGCCAAGC TGGCACTGGG AATTCCTCTG 
CCCGTTCTCA GAAACTCCGT GACCAACTCC ACCACAGCCA ACTTTGAGCC CAGCCTGGAT 
TACTGTGTGG TGAAGGTTCC GCGCTGGGAC CTGAGCAAGT TCTTGCGAGT CAGCACCAAG 
ATTGGCAGCT CCATGAAGAG TGTGGGGGAG GTCATGTCTG TTGGTAGGAA CTTTGAAGAA 
GCCTTCCAGA AAGCTCTGCG CATGGTGGAC GAGAACTGCG TGGGCTTTGA TCACTGCATC 
AAATCCGCAT CTGATGAGGA ACTGGAGACG CCGACAGACA AGAGGATCTT CGTTCTGGCC 
GCTGCCCTGC GTGCAGGTTA CACTATCGAG CGCCTTTATG AGTTGACCAA GATTGACAGG 
TGGTTCCTCC ATAAGATGAA GAATATTACT GATCAGGTGG CCACGCTGGA GTCCTACCAC 
CAGGATGAGA CCTTGATGCC CCGTGAGGTC CTTCTCCGAG CCAAACAGCT GGGCTTCTCC 
GACAAGCAGA TTGCCCTGGC CGTGCAGAGC ACGGAGCTGG CAGTCCGCAA GATGCGGCAG 
GAGCTGCTGA TTTTGCCGGT AGTAAAGCAG ATTGACACGG TGGCGGCGGA GTGGCCGGCT 
CAGACCAATT ACCTGTATCT GACCTATAAT GGAACTGAGC ACGACTTGGC CTTTGAGGAG 
CCGCATGTTA TGGTGATTGG CTCTGGTGTT TACCGAATAG GCAGCAGTGT GGAGTTTGAT 
TGGTGCGCGG TGGGCTGCAT CCAGGAACTG CGTAAGATGG GATTTAAGAC CATCATGGTG 
AATTATAACC CCGAGACTGT CAGCACAGAC TATGACATGT GTGACCGCCT GTATTTTGAC 
GAGATCTCAT TTGAGGTGGT GATGGATATC TATGAGCTGG AGAACCCAGA GGGAATCATA 
CTGTCCATGG GTGGCCAGCT GCCCAATAAT ATTGCCATGT CCCTACATCG CCAGCAGTGC 
CGGGTGCTGG GCACTTCTCC TGAGGCCATC GACTCCGCTG AGAACAGGTT TAAGTTTTCT 
CGCATGTTGG ACACGATCGG AATCAGTCAG CCGCAGTGGA AAGAGCTGTC TGACATGGAG 
TCGGCCGTTC AGTTCTGTAA CACCGTCGGT TTCCCCTGCG TGGTGCGCCC ATCCTACGTG 
CTAAGTGGAG CCGCCATGAA TGTGGCCCAT TCAGATGGAG ACCTGGAGAA GGTGCTCAGT 
AACGCTGTGG CTGTGTCCAA GGAGCATCCC GTGGTCATCT CTAAATTCAT CCAGGAGGCC 
AAGGAAATTG ATGTGGATGC TGTGGCATGT GATGGAGTTG TTGTGGCCGT GGCTATTTCA 
GAACATGTGG AGAATGCTGG GGTTCACTCT GGGGATGCCA CACTGGTGAC CCCTGCTCAG 
GATATCACCC CCAAAACCTT GGAAAGGATT AAAGCCATTG TCCATGCTGT GGGGCTGGAG 
CTCCAAGTCA ACGGACCCTT TAACCTGCAG CTCATTGCAA AGGACGACCA GCTGAAAGTG 
ATCGAGTGCA ATGTCCGTGT GTCGCGCTCC TTTCCCTTTG TATCGAAGAC TCTGGGAGTC 
AATCTAGTCG CTCTGGCAAC GCAAGTCATT ATGGGAGAAG AGGTTGAACC TGTTGGTGTG 
ATGACTGGTA CTGGCATTGT AGGGGTTAAG GTCCCACAGT TCTCGTTCTC ACGCTTGGCC 
GGCGCGGACG TGGTCCTTGG GGTGGAGATG ACCAGCACCG GGGAGGTTGC CTGCTTTGGA 
GAGAACCGAT ACGAGGCCTA TCTAAAAGCC ATGCTAAGTA CCGGCTTCAA AATTCCACAG 
AAGAACATCC TACTGACCAT CGGGAGCTAC AAGAACAAGA GTGAGCTGCT GCCCACGGTG 
CGAGCCATGG AGAGTCTTGG CTACAGTCTG TATGCCAGCC TGGGAACTGC TGACTTCTAT 
ACGGAGCATG GCATCAAGGT AAAGGCAATG GATTGGCCCT TTGAGGAGAG TGAGAATGAC 
GGGCCCCTAC GGGAGAAGCA GGGCAGCATC ATGGACCATC TGTCAGAGAA TCACTTTGAT 
TTGGTCATTA ATTTGTCCAT GCGGAATTCT GGGGGGCGCC GACTCTCCTC CTTCGTTACC 
CGTGGGTACC GTGCCCGCCG CTTGGCTGTG GACTATTCTG TGCCCCTCAT CATTGATATC 
AAGTGCACCA AACTCTTTGT GGAGGCTCTG CGTCAGATTG GGGGGGCGCC CCCTGTGAAG 
ACTCATGTCG ACTGTATGAC CTCACAGAAA CTTATCAGGC TGCCAGGCCT AGTGGACATC 
CACGTGCACC TGCGGGAACC TGGCGCTACT CATAAGGAGG ACTTTTCTTC TGGCACAGCA 
GCTGCCCTGG CTGGTGGAAT TACCCTTGTA TGTGCCATGC CCAACACCAG CCCAGCCATC 
ACTGATGCCA GTTCCTTCTC TCTGGCACAG AAGCTGGCAG AGGCCGGTGC CCGCTGTGAC 
TTTGCCCTCT TCCTCGGTGC TTCCTCAGAG AATGCCGCTT CTCTTTCTTC GATGGCCAGT 
TCAGCCGCCG GCCTTAAGAT GTATCTGAAC GACACCTATT CGCAGCTTAA GATGGACAAT 
GTTTCTGTGT GGATGGAGCA CTTTGAGAAG TGGCCGGCAC ACATGCCCAT TGTGGCCCAT 
GCAGAGAAAC AGACCGTGGC GGCAATTCTC ATGGTGGCTC AGCTGTACCA GCGCCCTGTG 
CACATTTGCC ACGTTGCAAA AAAAGAGGAG ATCCTTATTA TCAAAGCAGC CAAGCAGAAG 
GGAATCCAAG TTACATGCGA GGTGGCCCCT CATCACCTTT TCCTGTGTCT CGATGATCTG 
AAGAAAATCG GGGAGACACG GGGGCAAGTA CGTCCCATGT TGGGGACTCG GGAGGATCTA 
GAATCTCTGT GGGAGAACTT GGATATAATA GACTGTTTTG CCACTGACCA CGCCCCTCAC 
TCTGTGGAGG AGAAGGACGG TCCAAACCCA CCTCCAGGGT ATCCCGGCTT GGAGACCATG 
CTGCCTCTGC TACTCACAGC AGTCAGTGAG GGACGTCTGA CTATTGATGA TATCATTAAG 
CGCCTGTACG ACAACCCCCA GAAGATCTTC TCTTTGCCTG TGCAGGAGAA CACCTATGTC 
GAGGTGGATT TGGAGCAAGA GTGGGTGATC CCGTCACACA TGCAGTTTAC CAAGTCCAGA 
TGGAGCCCCT TTGCTGGAAT GAAGGTGAAA GGAGCAGTGC GGCGAGTTGT TCTCCGTGGG 
GAGGTGGCCT ACATTGATGG GCAGGTACTG GTACCCATGG GATATGGACA GGATGTCAGG 
AAATGGACCC CACCTGTTGT TGCCCAATCT CACCCAGTCG CCATTAAAGA GATCCCTAAG 
ACCCCAGAGC GACATCGCCA TGTTACGGTA CAAAGTGAGA CGATTCGCAG TCGAGCACCA 
AGCCCCCGCA GACCCTGTCA GTCCGGAGAC GGGCGCTTCC ACTTGCCTCC TCGAGTGCAT 
CGTGCATCAG ATCCTGGCTT CCCAGCTGAG GAGCAGAAAG AGAAGGCACC CAAGAGACCC 
GTGGAGCCAG AGCTGGTACC CATTCAGGAT GGCTATTCCC ACCCTCCTCC CATCCCCAGG 
ATTATGTCTC CCCAGAGTAC GTCCATGCAA AGTGCCCCCC ACGTGCAGAC CTCACCCCTG 
TTGCACCCGT TGATTGGCCA ACACATCCTG ACGGTTAAAC AGTTCAGCAA AGACCAGATG 
TCGCATCTGT TCAACGTGGC GCACACGCTG CGCATGCTGG TGCAGAAGGA GAGGAACCTT 
GATATCCTAA AGGGCAAGGT GATGGCCTCC ATGTTTTTTG AGGCAAGCAC ACGGACAAGC 
AGCTCTTTTG CTGCAGCTAT GCAACGCCTT GGGGGCTCTG TGCTCAGTTT CTGTGAGACA 
ACCTCCTCTT CTCAAAAAGG AGAGTCCCTC ACCGACTCTG TTCAGACAAT GAGCTGCTAT 
TCGGATGTCC TTGTGGTGAG ACACCCAGAG CCTGGAGCTG TGGAGCTTGC TGCCAAGCAC 
TGCCGGAAGC CTGTGATAAA TGCTGGGGAT GGCGTCGGCG AGCACCCCAC TCAGGCCCTA 
CTGGACATTT TCACAATCCG TGAAGAGCTG GGCACAGTCA ATGGAATGAT AATAACGATG 
GTGGGAGATC TGAAACATGG CCGGACAGTT CATTCCCTCG CCTGCCTCTT AACACAGTAC 
CGTGTGACCT TGAGATACGT GACTCCCCGC AGCCTCCGTA TGCCCTCCAA CATCATACAC 
TTTGTAGCCT CCAAGGGCAT TAAGCAGGAA GAGTTTGAGA GCATTGAAGA GGCTCTGCCA 
GATACAGATG TGTTGTATAT GACCCGAATA CAAAAGGAGA GATTTAATTC TACTGACGAA 
TACAATGCTT GCTTTGGCCA GTTTATCCTG ACGCCGCACA TCATGACCAG GGCCAAGAAG 
AAAATGGTGG TAATGCATCC GATGCCTCGT GTGAATGAGA TCAGCTTTGA AGTGGATTCC 
GACCCTCGGG CAGCTTACTT TCGCCAGGCT GAGAATGGCA TGTATATCCG CATGGCTCTG 
CTAGCAACGG TTTTGGGAAA ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757790.1
CDS	112..6858
Translation	MAAGTAVLLLEDGTRVSGTVFGAVRPVSGEVVFQTGMVGYPEALTDPSYRAQILVLTYPLIGNYGIPQDELDQFGLSRWFESSKIHASALIIGERSRNPSHWSCDISLNQWLKQQGIPGLEGVDTRALTKKIREKGTLLGKLIPVGTEESQVPYEDPAKRNLVQEVSLKEPRVYNAGGTVRIAALDCGLKFNQIRCLCQRGAEVTVLPWDYPISSAECDGLFLSNGPGDPTFCQSSVRNIRDFISEEKPKPVFGICLGHQILSLVIGAHTYKMKYGNRGHNQPCIHESSRRCFITSQNHGYAVDPNTLPEGWSPLFTNANDQSNEGIIHDSKPFFSVQFHPEHCAGPMDLVGLFDVFLDTVRDRKAGDSGEKTVKQRVTELLTYKESPSVTDLEASRPHKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTVLINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGILLTFGGQTALNCGVELTKQGVLEKYNVRVLGTPVKSIEMTEDRKVFVEKMEEIGEHVAPSEAAFSLEQAQAAAERLGYPVLVRSAYALGGLGSGFASTKEELTALVCQAFAHTTQVLVDKSLKGWKEIEYEVVRDAYNNCVTVCNMENVDPLGIHTGESIVVAPSQTLNDREYNMLRNTAIKVIQHLGIVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPVLRNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMSVGRNFEEAFQKALRMVDENCVGFDHCIKSASDEELETPTDKRIFVLAAALRAGYTIERLYELTKIDRWFLHKMKNITDQVATLESYHQDETLMPREVLLRAKQLGFSDKQIALAVQSTELAVRKMRQELLILPVVKQIDTVAAEWPAQTNYLYLTYNGTEHDLAFEEPHVMVIGSGVYRIGSSVEFDWCAVGCIQELRKMGFKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGIILSMGGQLPNNIAMSLHRQQCRVLGTSPEAIDSAENRFKFSRMLDTIGISQPQWKELSDMESAVQFCNTVGFPCVVRPSYVLSGAAMNVAHSDGDLEKVLSNAVAVSKEHPVVISKFIQEAKEIDVDAVACDGVVVAVAISEHVENAGVHSGDATLVTPAQDITPKTLERIKAIVHAVGLELQVNGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVNLVALATQVIMGEEVEPVGVMTGTGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPQKNILLTIGSYKNKSELLPTVRAMESLGYSLYASLGTADFYTEHGIKVKAMDWPFEESENDGPLREKQGSIMDHLSENHFDLVINLSMRNSGGRRLSSFVTRGYRARRLAVDYSVPLIIDIKCTKLFVEALRQIGGAPPVKTHVDCMTSQKLIRLPGLVDIHVHLREPGATHKEDFSSGTAAALAGGITLVCAMPNTSPAITDASSFSLAQKLAEAGARCDFALFLGASSENAASLSSMASSAAGLKMYLNDTYSQLKMDNVSVWMEHFEKWPAHMPIVAHAEKQTVAAILMVAQLYQRPVHICHVAKKEEILIIKAAKQKGIQVTCEVAPHHLFLCLDDLKKIGETRGQVRPMLGTREDLESLWENLDIIDCFATDHAPHSVEEKDGPNPPPGYPGLETMLPLLLTAVSEGRLTIDDIIKRLYDNPQKIFSLPVQENTYVEVDLEQEWVIPSHMQFTKSRWSPFAGMKVKGAVRRVVLRGEVAYIDGQVLVPMGYGQDVRKWTPPVVAQSHPVAIKEIPKTPERHRHVTVQSETIRSRAPSPRRPCQSGDGRFHLPPRVHRASDPGFPAEEQKEKAPKRPVEPELVPIQDGYSHPPPIPRIMSPQSTSMQSAPHVQTSPLLHPLIGQHILTVKQFSKDQMSHLFNVAHTLRMLVQKERNLDILKGKVMASMFFEASTRTSSSFAAAMQRLGGSVLSFCETTSSSQKGESLTDSVQTMSCYSDVLVVRHPEPGAVELAAKHCRKPVINAGDGVGEHPTQALLDIFTIREELGTVNGMIITMVGDLKHGRTVHSLACLLTQYRVTLRYVTPRSLRMPSNIIHFVASKGIKQEEFESIEEALPDTDVLYMTRIQKERFNSTDEYNACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISFEVDSDPRAAYFRQAENGMYIRMALLATVLGKY

Target ORF information:

RefSeq Version	XM_031901930.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901930.1

ORF Insert Sequence:

ATGGCTGCGG GTACTGCGGT GCTACTGCTG GAGGATGGAA CCCGGGTATC CGGAACCGTG 
TTCGGGGCGG TGCGTCCGGT GTCCGGGGAA GTGGTGTTCC AGACGGGCAT GGTGGGCTAC 
CCCGAGGCCC TCACCGACCC CTCCTACAGG GCCCAGATCC TGGTACTGAC ATATCCGCTG 
ATTGGGAACT ACGGGATCCC GCAGGATGAA CTGGACCAGT TTGGCCTCAG TCGGTGGTTC 
GAGTCCAGCA AGATTCACGC CTCGGCGCTG ATTATTGGGG AGCGGTCCCG GAACCCCAGT 
CACTGGAGCT GTGACATCTC ATTGAACCAG TGGCTCAAAC AGCAGGGAAT CCCCGGACTG 
GAAGGTGTGG ATACCCGGGC ACTCACCAAG AAGATCCGAG AGAAGGGCAC CCTGCTGGGC 
AAGCTCATAC CAGTGGGCAC AGAGGAGAGC CAGGTCCCTT ATGAAGATCC TGCCAAGAGG 
AACCTGGTTC AGGAGGTGTC ATTGAAGGAG CCCCGAGTGT ACAATGCGGG GGGCACAGTG 
AGAATCGCTG CCCTGGACTG TGGTTTGAAG TTCAACCAGA TTCGCTGCCT GTGCCAGAGG 
GGGGCCGAGG TGACGGTGCT GCCATGGGAC TATCCAATCA GCAGTGCAGA GTGTGACGGC 
CTGTTCCTCA GTAACGGCCC CGGGGACCCC ACGTTCTGTC AGAGCTCCGT GCGGAACATC 
AGGGACTTCA TTTCCGAGGA GAAGCCGAAA CCCGTGTTTG GGATCTGTCT GGGCCACCAG 
ATCCTCTCTC TGGTTATAGG CGCCCACACC TACAAGATGA AGTACGGGAA TCGTGGGCAC 
AACCAGCCGT GCATCCATGA GAGCTCTCGG CGCTGCTTCA TAACCTCACA GAACCATGGA 
TACGCCGTGG ACCCAAACAC TTTACCGGAA GGCTGGTCTC CTCTGTTCAC GAATGCCAAC 
GACCAATCAA ATGAGGGAAT TATCCATGAT TCTAAGCCCT TTTTCAGTGT GCAGTTCCAC 
CCCGAGCACT GTGCTGGGCC CATGGATCTG GTCGGCCTGT TTGATGTGTT CCTGGACACT 
GTGAGAGACC GGAAGGCTGG AGATTCAGGA GAGAAGACGG TTAAACAGCG AGTGACTGAG 
CTGCTCACAT ACAAGGAGTC TCCCAGTGTC ACAGACCTAG AGGCCTCTCG CCCACACAAA 
GTGCTGATCC TGGGCTCAGG GGGGCTGTCG ATTGGCCAGG CCGGGGAGTT CGACTACTCT 
GGCTCCCAGG CCATCAAAGC TCTGAAGGAA GAGAACATCC AGACTGTCCT GATTAACCCC 
AACATCGCCA CAGTGCAGAC ATCGAAAGGC CTCGCCGACA AGGTTTATTT CCTGCCAATC 
ACCCCTGAAT ATGTCACTCA GGTGATAAAG AATGAGCGGC CAGATGGGAT CCTCCTGACG 
TTTGGGGGTC AGACTGCATT GAACTGTGGG GTGGAGCTGA CCAAGCAGGG AGTCCTAGAG 
AAGTACAATG TGCGGGTTCT GGGGACCCCT GTGAAGTCCA TTGAAATGAC GGAAGATCGC 
AAAGTGTTTG TGGAGAAGAT GGAGGAGATT GGAGAACACG TGGCCCCCAG TGAGGCAGCT 
TTCTCCTTAG AGCAGGCCCA GGCTGCTGCA GAACGTCTCG GGTATCCGGT TTTGGTCCGG 
TCGGCCTACG CGCTGGGCGG CCTTGGATCT GGATTTGCCA GTACCAAGGA GGAGTTGACT 
GCCCTGGTGT GCCAGGCCTT CGCCCACACC ACACAGGTCC TGGTGGATAA ATCCCTGAAG 
GGCTGGAAGG AGATAGAGTA CGAAGTCGTG CGGGACGCCT ATAATAACTG TGTCACGGTG 
TGCAACATGG AGAACGTAGA TCCCCTGGGC ATTCACACTG GCGAGTCCAT CGTGGTAGCT 
CCCAGCCAGA CGCTGAACGA TCGAGAGTAC AACATGCTGC GCAACACGGC CATTAAGGTT 
ATCCAGCACC TCGGCATTGT GGGCGAGTGT AACATACAGT ACGCCCTAAA CCCGGAGTCT 
GAGCAGTATT ACATCATCGA GGTGAACGCA CGGCTGTCTC GCAGCTCAGC CCTGGCGAGT 
AAAGCAACTG GTTACCCCCT GGCGTATGTG GCTGCCAAGC TGGCACTGGG AATTCCTCTG 
CCCGTTCTCA GAAACTCCGT GACCAACTCC ACCACAGCCA ACTTTGAGCC CAGCCTGGAT 
TACTGTGTGG TGAAGGTTCC GCGCTGGGAC CTGAGCAAGT TCTTGCGAGT CAGCACCAAG 
ATTGGCAGCT CCATGAAGAG TGTGGGGGAG GTCATGTCTG TTGGTAGGAA CTTTGAAGAA 
GCCTTCCAGA AAGCTCTGCG CATGGTGGAC GAGAACTGCG TGGGCTTTGA TCACTGCATC 
AAATCCGCAT CTGATGAGGA ACTGGAGACG CCGACAGACA AGAGGATCTT CGTTCTGGCC 
GCTGCCCTGC GTGCAGGTTA CACTATCGAG CGCCTTTATG AGTTGACCAA GATTGACAGG 
TGGTTCCTCC ATAAGATGAA GAATATTACT GATCAGGTGG CCACGCTGGA GTCCTACCAC 
CAGGATGAGA CCTTGATGCC CCGTGAGGTC CTTCTCCGAG CCAAACAGCT GGGCTTCTCC 
GACAAGCAGA TTGCCCTGGC CGTGCAGAGC ACGGAGCTGG CAGTCCGCAA GATGCGGCAG 
GAGCTGCTGA TTTTGCCGGT AGTAAAGCAG ATTGACACGG TGGCGGCGGA GTGGCCGGCT 
CAGACCAATT ACCTGTATCT GACCTATAAT GGAACTGAGC ACGACTTGGC CTTTGAGGAG 
CCGCATGTTA TGGTGATTGG CTCTGGTGTT TACCGAATAG GCAGCAGTGT GGAGTTTGAT 
TGGTGCGCGG TGGGCTGCAT CCAGGAACTG CGTAAGATGG GATTTAAGAC CATCATGGTG 
AATTATAACC CCGAGACTGT CAGCACAGAC TATGACATGT GTGACCGCCT GTATTTTGAC 
GAGATCTCAT TTGAGGTGGT GATGGATATC TATGAGCTGG AGAACCCAGA GGGAATCATA 
CTGTCCATGG GTGGCCAGCT GCCCAATAAT ATTGCCATGT CCCTACATCG CCAGCAGTGC 
CGGGTGCTGG GCACTTCTCC TGAGGCCATC GACTCCGCTG AGAACAGGTT TAAGTTTTCT 
CGCATGTTGG ACACGATCGG AATCAGTCAG CCGCAGTGGA AAGAGCTGTC TGACATGGAG 
TCGGCCGTTC AGTTCTGTAA CACCGTCGGT TTCCCCTGCG TGGTGCGCCC ATCCTACGTG 
CTAAGTGGAG CCGCCATGAA TGTGGCCCAT TCAGATGGAG ACCTGGAGAA GGTGCTCAGT 
AACGCTGTGG CTGTGTCCAA GGAGCATCCC GTGGTCATCT CTAAATTCAT CCAGGAGGCC 
AAGGAAATTG ATGTGGATGC TGTGGCATGT GATGGAGTTG TTGTGGCCGT GGCTATTTCA 
GAACATGTGG AGAATGCTGG GGTTCACTCT GGGGATGCCA CACTGGTGAC CCCTGCTCAG 
GATATCACCC CCAAAACCTT GGAAAGGATT AAAGCCATTG TCCATGCTGT GGGGCTGGAG 
CTCCAAGTCA ACGGACCCTT TAACCTGCAG CTCATTGCAA AGGACGACCA GCTGAAAGTG 
ATCGAGTGCA ATGTCCGTGT GTCGCGCTCC TTTCCCTTTG TATCGAAGAC TCTGGGAGTC 
AATCTAGTCG CTCTGGCAAC GCAAGTCATT ATGGGAGAAG AGGTTGAACC TGTTGGTGTG 
ATGACTGGTA CTGGCATTGT AGGGGTTAAG GTCCCACAGT TCTCGTTCTC ACGCTTGGCC 
GGCGCGGACG TGGTCCTTGG GGTGGAGATG ACCAGCACCG GGGAGGTTGC CTGCTTTGGA 
GAGAACCGAT ACGAGGCCTA TCTAAAAGCC ATGCTAAGTA CCGGCTTCAA AATTCCACAG 
AAGAACATCC TACTGACCAT CGGGAGCTAC AAGAACAAGA GTGAGCTGCT GCCCACGGTG 
CGAGCCATGG AGAGTCTTGG CTACAGTCTG TATGCCAGCC TGGGAACTGC TGACTTCTAT 
ACGGAGCATG GCATCAAGGT AAAGGCAATG GATTGGCCCT TTGAGGAGAG TGAGAATGAC 
GGGCCCCTAC GGGAGAAGCA GGGCAGCATC ATGGACCATC TGTCAGAGAA TCACTTTGAT 
TTGGTCATTA ATTTGTCCAT GCGGAATTCT GGGGGGCGCC GACTCTCCTC CTTCGTTACC 
CGTGGGTACC GTGCCCGCCG CTTGGCTGTG GACTATTCTG TGCCCCTCAT CATTGATATC 
AAGTGCACCA AACTCTTTGT GGAGGCTCTG CGTCAGATTG GGGGGGCGCC CCCTGTGAAG 
ACTCATGTCG ACTGTATGAC CTCACAGAAA CTTATCAGGC TGCCAGGCCT AGTGGACATC 
CACGTGCACC TGCGGGAACC TGGCGCTACT CATAAGGAGG ACTTTTCTTC TGGCACAGCA 
GCTGCCCTGG CTGGTGGAAT TACCCTTGTA TGTGCCATGC CCAACACCAG CCCAGCCATC 
ACTGATGCCA GTTCCTTCTC TCTGGCACAG AAGCTGGCAG AGGCCGGTGC CCGCTGTGAC 
TTTGCCCTCT TCCTCGGTGC TTCCTCAGAG AATGCCGCTT CTCTTTCTTC GATGGCCAGT 
TCAGCCGCCG GCCTTAAGAT GTATCTGAAC GACACCTATT CGCAGCTTAA GATGGACAAT 
GTTTCTGTGT GGATGGAGCA CTTTGAGAAG TGGCCGGCAC ACATGCCCAT TGTGGCCCAT 
GCAGAGAAAC AGACCGTGGC GGCAATTCTC ATGGTGGCTC AGCTGTACCA GCGCCCTGTG 
CACATTTGCC ACGTTGCAAA AAAAGAGGAG ATCCTTATTA TCAAAGCAGC CAAGCAGAAG 
GGAATCCAAG TTACATGCGA GGTGGCCCCT CATCACCTTT TCCTGTGTCT CGATGATCTG 
AAGAAAATCG GGGAGACACG GGGGCAAGTA CGTCCCATGT TGGGGACTCG GGAGGATCTA 
GAATCTCTGT GGGAGAACTT GGATATAATA GACTGTTTTG CCACTGACCA CGCCCCTCAC 
TCTGTGGAGG AGAAGGACGG TCCAAACCCA CCTCCAGGGT ATCCCGGCTT GGAGACCATG 
CTGCCTCTGC TACTCACAGC AGTCAGTGAG GGACGTCTGA CTATTGATGA TATCATTAAG 
CGCCTGTACG ACAACCCCCA GAAGATCTTC TCTTTGCCTG TGCAGGAGAA CACCTATGTC 
GAGGTGGATT TGGAGCAAGA GTGGGTGATC CCGTCACACA TGCAGTTTAC CAAGTCCAGA 
TGGAGCCCCT TTGCTGGAAT GAAGGTGAAA GGAGCAGTGC GGCGAGTTGT TCTCCGTGGG 
GAGGTGGCCT ACATTGATGG GCAGGTACTG GTACCCATGG GATATGGACA GGATGTCAGG 
AAATGGACCC CACCTGTTGT TGCCCAATCT CACCCAGTCG CCATTAAAGA GATCCCTAAG 
ACCCCAGAGC GACATCGCCA TGTTACGGTA CAAAGTGAGA CGATTCGCAG TCGAGCACCA 
AGCCCCCGCA GACCCTGTCA GTCCGGAGAC GGGCGCTTCC ACTTGCCTCC TCGAGTGCAT 
CGTGCATCAG ATCCTGGCTT CCCAGCTGAG GAGCAGAAAG AGAAGGCACC CAAGAGACCC 
GTGGAGCCAG AGCTGGTACC CATTCAGGAT GGCTATTCCC ACCCTCCTCC CATCCCCAGG 
ATTATGTCTC CCCAGAGTAC GTCCATGCAA AGTGCCCCCC ACGTGCAGAC CTCACCCCTG 
TTGCACCCGT TGATTGGCCA ACACATCCTG ACGGTTAAAC AGTTCAGCAA AGACCAGATG 
TCGCATCTGT TCAACGTGGC GCACACGCTG CGCATGCTGG TGCAGAAGGA GAGGAACCTT 
GATATCCTAA AGGGCAAGGT GATGGCCTCC ATGTTTTTTG AGGCAAGCAC ACGGACAAGC 
AGCTCTTTTG CTGCAGCTAT GCAACGCCTT GGGGGCTCTG TGCTCAGTTT CTGTGAGACA 
ACCTCCTCTT CTCAAAAAGG AGAGTCCCTC ACCGACTCTG TTCAGACAAT GAGCTGCTAT 
TCGGATGTCC TTGTGGTGAG ACACCCAGAG CCTGGAGCTG TGGAGCTTGC TGCCAAGCAC 
TGCCGGAAGC CTGTGATAAA TGCTGGGGAT GGCGTCGGCG AGCACCCCAC TCAGGCCCTA 
CTGGACATTT TCACAATCCG TGAAGAGCTG GGCACAGTCA ATGGAATGAT AATAACGATG 
GTGGGAGATC TGAAACATGG CCGGACAGTT CATTCCCTCG CCTGCCTCTT AACACAGTAC 
CGTGTGACCT TGAGATACGT GACTCCCCGC AGCCTCCGTA TGCCCTCCAA CATCATACAC 
TTTGTAGCCT CCAAGGGCAT TAAGCAGGAA GAGTTTGAGA GCATTGAAGA GGCTCTGCCA 
GATACAGATG TGTTGTATAT GACCCGAATA CAAAAGGAGA GATTTAATTC TACTGACGAA 
TACAATGCTT GCTTTGGCCA GTTTATCCTG ACGCCGCACA TCATGACCAG GGCCAAGAAG 
AAAATGGTGG TAATGCATCC GATGCCTCGT GTGAATGAGA TCAGCTTTGA AGTGGATTCC 
GACCCTCGGG CAGCTTACTT TCGCCAGGCT GAGAATGGCA TGTATATCCG CATGGCTCTG 
CTAGCAACGG TTTTGGGAAA ATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cad ( XM_031901931.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757791.1 Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.

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CloneID	OXa58216
Clone ID Related Accession (Same CDS sequence)	XM_031901931.1
Accession Version	XM_031901931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG GTACTGCGGT GCTACTGCTG GAGGATGGAA CCCGGGTATC CGGAACCGTG 
TTCGGGGCGG TGCGTCCGGT GTCCGGGGAA GTGGTGTTCC AGACGGGCAT GGTGGGCTAC 
CCCGAGGCCC TCACCGACCC CTCCTACAGG GCCCAGATCC TGGTACTGAC ATATCCGCTG 
ATTGGGAACT ACGGGATCCC GCAGGATGAA CTGGACCAGT TTGGCCTCAG TCGGTGGTTC 
GAGTCCAGCA AGATTCACGC CTCGGCGCTG ATTATTGGGG AGCGGTCCCG GAACCCCAGT 
CACTGGAGCT GTGACATCTC ATTGAACCAG TGGCTCAAAC AGCAGGGAAT CCCCGGACTG 
GAAGGTGTGG ATACCCGGGC ACTCACCAAG AAGATCCGAG AGAAGGGCAC CCTGCTGGGC 
AAGCTCATAC CAGTGGGCAC AGAGGAGAGC CAGGTCCCTT ATGAAGATCC TGCCAAGAGG 
AACCTGGTTC AGGAGGTGTC ATTGAAGGAG CCCCGAGTGT ACAATGCGGG GGGCACAGTG 
AGAATCGCTG CCCTGGACTG TGGTTTGAAG TTCAACCAGA TTCGCTGCCT GTGCCAGAGG 
GGGGCCGAGG TGACGGTGCT GCCATGGGAC TATCCAATCA GCAGTGCAGA GTGTGACGGC 
CTGTTCCTCA GTAACGGCCC CGGGGACCCC ACGTTCTGTC AGAGCTCCGT GCGGAACATC 
AGGGACTTCA TTTCCGAGGA GAAGCCGAAA CCCGTGTTTG GGATCTGTCT GGGCCACCAG 
ATCCTCTCTC TGGTTATAGG CGCCCACACC TACAAGATGA AGTACGGGAA TCGTGGGCAC 
AACCAGCCGT GCATCCATGA GAGCTCTCGG CGCTGCTTCA TAACCTCACA GAACCATGGA 
TACGCCGTGG ACCCAAACAC TTTACCGGAA GGCTGGTCTC CTCTGTTCAC GAATGCCAAC 
GACCAATCAA ATGAGGGAAT TATCCATGAT TCTAAGCCCT TTTTCAGTGT GCAGTTCCAC 
CCCGAGCACT GTGCTGGGCC CATGGATCTG GTCGGCCTGT TTGATGTGTT CCTGGACACT 
GTGAGAGACC GGAAGGCTGG AGATTCAGGA GAGAAGACGG TTAAACAGCG AGTGACTGAG 
CTGCTCACAT ACAAGGAGTC TCCCAGTGTC ACAGACCTAG AGGCCTCTCG CCCACACAAA 
GTGCTGATCC TGGGCTCAGG GGGGCTGTCG ATTGGCCAGG CCGGGGAGTT CGACTACTCT 
GGCTCCCAGG CCATCAAAGC TCTGAAGGAA GAGAACATCC AGACTGTCCT GATTAACCCC 
AACATCGCCA CAGTGCAGAC ATCGAAAGGC CTCGCCGACA AGGTTTATTT CCTGCCAATC 
ACCCCTGAAT ATGTCACTCA GGTGATAAAG AATGAGCGGC CAGATGGGAT CCTCCTGACG 
TTTGGGGGTC AGACTGCATT GAACTGTGGG GTGGAGCTGA CCAAGCAGGG AGTCCTAGAG 
AAGTACAATG TGCGGGTTCT GGGGACCCCT GTGAAGTCCA TTGAAATGAC GGAAGATCGC 
AAAGTGTTTG TGGAGAAGAT GGAGGAGATT GGAGAACACG TGGCCCCCAG TGAGGCAGCT 
TTCTCCTTAG AGCAGGCCCA GGCTGCTGCA GAACGTCTCG GGTATCCGGT TTTGGTCCGG 
TCGGCCTACG CGCTGGGCGG CCTTGGATCT GGATTTGCCA GTACCAAGGA GGAGTTGACT 
GCCCTGGTGT GCCAGGCCTT CGCCCACACC ACACAGGTCC TGGTGGATAA ATCCCTGAAG 
GGCTGGAAGG AGATAGAGTA CGAAGTCGTG CGGGACGCCT ATAATAACTG TGTCACGGTG 
TGCAACATGG AGAACGTAGA TCCCCTGGGC ATTCACACTG GCGAGTCCAT CGTGGTAGCT 
CCCAGCCAGA CGCTGAACGA TCGAGAGTAC AACATGCTGC GCAACACGGC CATTAAGGTT 
ATCCAGCACC TCGGCATTGT GGGCGAGTGT AACATACAGT ACGCCCTAAA CCCGGAGTCT 
GAGCAGTATT ACATCATCGA GGTGAACGCA CGGCTGTCTC GCAGCTCAGC CCTGGCGAGT 
AAAGCAACTG GTTACCCCCT GGCGTATGTG GCTGCCAAGC TGGCACTGGG AATTCCTCTG 
CCCGTTCTCA GAAACTCCGT GACCAACTCC ACCACAGCCA ACTTTGAGCC CAGCCTGGAT 
TACTGTGTGG TGAAGGTTCC GCGCTGGGAC CTGAGCAAGT TCTTGCGAGT CAGCACCAAG 
ATTGGCAGCT CCATGAAGAG TGTGGGGGAG GTCATGTCTG TTGGTAGGAA CTTTGAAGAA 
GCCTTCCAGA AAGCTCTGCG CATGGTGGAC GAGAACTGCG TGGGCTTTGA TCACTGCATC 
AAATCCGCAT CTGATGAGGA ACTGGAGACG CCGACAGACA AGAGGATCTT CGTTCTGGCC 
GCTGCCCTGC GTGCAGGTTA CACTATCGAG CGCCTTTATG AGTTGACCAA GATTGACAGG 
TGGTTCCTCC ATAAGATGAA GAATATTACT GATCAGGTGG CCACGCTGGA GTCCTACCAC 
CAGGATGAGA CCTTGATGCC CCGTGAGGTC CTTCTCCGAG CCAAACAGCT GGGCTTCTCC 
GACAAGCAGA TTGCCCTGGC CGTGCAGAGC ACGGAGCTGG CAGTCCGCAA GATGCGGCAG 
GAGCTGCTGA TTTTGCCGGT AGTAAAGCAG ATTGACACGG TGGCGGCGGA GTGGCCGGCT 
CAGACCAATT ACCTGTATCT GACCTATAAT GGAACTGAGC ACGACTTGGC CTTTGAGGAG 
CCGCATGTTA TGGTGATTGG CTCTGGTGTT TACCGAATAG GCAGCAGTGT GGAGTTTGAT 
TGGTGCGCGG TGGGCTGCAT CCAGGAACTG CGTAAGATGG GATTTAAGAC CATCATGGTG 
AATTATAACC CCGAGACTGT CAGCACAGAC TATGACATGT GTGACCGCCT GTATTTTGAC 
GAGATCTCAT TTGAGGTGGT GATGGATATC TATGAGCTGG AGAACCCAGA GGGAATCATA 
CTGTCCATGG GTGGCCAGCT GCCCAATAAT ATTGCCATGT CCCTACATCG CCAGCAGTGC 
CGGGTGCTGG GCACTTCTCC TGAGGCCATC GACTCCGCTG AGAACAGGTT TAAGTTTTCT 
CGCATGTTGG ACACGATCGG AATCAGTCAG CCGCAGTGGA AAGAGCTGTC TGACATGGAG 
TCGGCCGTTC AGTTCTGTAA CACCGTCGGT TTCCCCTGCG TGGTGCGCCC ATCCTACGTG 
CTAAGTGGAG CCGCCATGAA TGTGGCCCAT TCAGATGGAG ACCTGGAGAA GGTGCTCAGT 
AACGCTGTGG CTGTGTCCAA GGAGCATCCC GTGGTCATCT CTAAATTCAT CCAGGAGGCC 
AAGGAAATTG ATGTGGATGC TGTGGCATGT GATGGAGTTG TTGTGGCCGT GGCTATTTCA 
GAACATGTGG AGAATGCTGG GGTTCACTCT GGGGATGCCA CACTGGTGAC CCCTGCTCAG 
GATATCACCC CCAAAACCTT GGAAAGGATT AAAGCCATTG TCCATGCTGT GGGGCTGGAG 
CTCCAAGTCA ACGGACCCTT TAACCTGCAG CTCATTGCAA AGGACGACCA GCTGAAAGTG 
ATCGAGTGCA ATGTCCGTGT GTCGCGCTCC TTTCCCTTTG TATCGAAGAC TCTGGGAGTC 
AATCTAGTCG CTCTGGCAAC GCAAGTCATT ATGGGAGAAG AGGTTGAACC TGTTGGTGTG 
ATGACTGGTA CTGGCATTGT AGGGGTTAAG GTCCCACAGT TCTCGTTCTC ACGCTTGGCC 
GGCGCGGACG TGGTCCTTGG GGTGGAGATG ACCAGCACCG GGGAGGTTGC CTGCTTTGGA 
GAGAACCGAT ACGAGGCCTA TCTAAAAGCC ATGCTAAGTA CCGGCTTCAA AATTCCACAG 
AAGAACATCC TACTGACCAT CGGGAGCTAC AAGAACAAGA GTGAGCTGCT GCCCACGGTG 
CGAGCCATGG AGAGTCTTGG CTACAGTCTG TATGCCAGCC TGGGAACTGC TGACTTCTAT 
ACGGAGCATG GCATCAAGGT AAAGGCAATG GATTGGCCCT TTGAGGAGAG TGAGAATGAC 
GGGCCCCTAC GGGAGAAGCA GGGCAGCATC ATGGACCATC TGTCAGAGAA TCACTTTGAT 
TTGGTCATTA ATTTGTCCAT GCGGAATTCT GGGGGGCGCC GACTCTCCTC CTTCGTTACC 
CGTGGGTACC GTGCCCGCCG CTTGGCTGTG GACTATTCTG TGCCCCTCAT CATTGATATC 
AAGTGCACCA AACTCTTTGT GGAGGCTCTG CGTCAGATTG GGGGGGCGCC CCCTGTGAAG 
ACTCATGTCG ACTGTATGAC CTCACAGAAA CTTATCAGGC TGCCAGGCCT AGTGGACATC 
CACGTGCACC TGCGGGAACC TGGCGCTACT CATAAGGAGG ACTTTTCTTC TGGCACAGCA 
GCTGCCCTGG CTGGTGGAAT TACCCTTGTA TGTGCCATGC CCAACACCAG CCCAGCCATC 
ACTGATGCCA GTTCCTTCTC TCTGGCACAG AAGCTGGCAG AGGCCGGTGC CCGCTGTGAC 
TTTGCCCTCT TCCTCGGTGC TTCCTCAGAG AATGCCGCTT CTCTTTCTTC GATGGCCAGT 
TCAGCCGCCG GCCTTAAGAT GTATCTGAAC GACACCTATT CGCAGCTTAA GATGGACAAT 
GTTTCTGTGT GGATGGAGCA CTTTGAGAAG TGGCCGGCAC ACATGCCCAT TGTGGCCCAT 
GCAGAGAAAC AGACCGTGGC GGCAATTCTC ATGGTGGCTC AGCTGTACCA GCGCCCTGTG 
CACATTTGCC ACGTTGCAAA AAAAGAGGAG ATCCTTATTA TCAAAGCAGC CAAGCAGAAG 
GGAATCCAAG TTACATGCGA GGTGGCCCCT CATCACCTTT TCCTGTGTCT CGATGATCTG 
AAGAAAATCG GGGAGACACG GGGGCAAGTA CGTCCCATGT TGGGGACTCG GGAGGATCTA 
GAATCTCTGT GGGAGAACTT GGATATAATA GACTGTTTTG CCACTGACCA CGCCCCTCAC 
TCTGTGGAGG AGAAGGACGG TCCAAACCCA CCTCCAGGGT ATCCCGGCTT GGAGACCATG 
CTGCCTCTGC TACTCACAGC AGTCAGTGAG GGACGTCTGA CTATTGATGA TATCATTAAG 
CGCCTGTACG ACAACCCCCA GAAGATCTTC TCTTTGCCTG TGCAGGAGAA CACCTATGTC 
GAGGTGGATT TGGAGCAAGA GTGGGTGATC CCGTCACACA TGCAGTTTAC CAAGTCCAGA 
TGGAGCCCCT TTGCTGGAAT GAAGGTGAAA GGAGCAGTGC GGCGAGTTGT TCTCCGTGGG 
GAGGTGGCCT ACATTGATGG GCAGGTACTG GTACCCATGG GATATGGACA GGATGTCAGG 
AAATGGACCC CACCTGTTGT TGCCCAATCT CACCCAGTCG CCATTAAAGA GATCCCTAAG 
ACCCCAGAGC GACATCGCCA TGTTACGGTA CAAAGTGAGA CGATTCGCAG TCGAGCACCA 
AGCCCCCGCA GACCCTGTCA GTCCGGAGAC GGGCGCTTCC ACTTGCCTCC TCGAGTGCAT 
CGTGCATCAG ATCCTGGCTT CCCAGAGCTG GTACCCATTC AGGATGGCTA TTCCCACCCT 
CCTCCCATCC CCAGGATTAT GTCTCCCCAG AGTACGTCCA TGCAAAGTGC CCCCCACGTG 
CAGACCTCAC CCCTGTTGCA CCCGTTGATT GGCCAACACA TCCTGACGGT TAAACAGTTC 
AGCAAAGACC AGATGTCGCA TCTGTTCAAC GTGGCGCACA CGCTGCGCAT GCTGGTGCAG 
AAGGAGAGGA ACCTTGATAT CCTAAAGGGC AAGGTGATGG CCTCCATGTT TTTTGAGGCA 
AGCACACGGA CAAGCAGCTC TTTTGCTGCA GCTATGCAAC GCCTTGGGGG CTCTGTGCTC 
AGTTTCTGTG AGACAACCTC CTCTTCTCAA AAAGGAGAGT CCCTCACCGA CTCTGTTCAG 
ACAATGAGCT GCTATTCGGA TGTCCTTGTG GTGAGACACC CAGAGCCTGG AGCTGTGGAG 
CTTGCTGCCA AGCACTGCCG GAAGCCTGTG ATAAATGCTG GGGATGGCGT CGGCGAGCAC 
CCCACTCAGG CCCTACTGGA CATTTTCACA ATCCGTGAAG AGCTGGGCAC AGTCAATGGA 
ATGATAATAA CGATGGTGGG AGATCTGAAA CATGGCCGGA CAGTTCATTC CCTCGCCTGC 
CTCTTAACAC AGTACCGTGT GACCTTGAGA TACGTGACTC CCCGCAGCCT CCGTATGCCC 
TCCAACATCA TACACTTTGT AGCCTCCAAG GGCATTAAGC AGGAAGAGTT TGAGAGCATT 
GAAGAGGCTC TGCCAGATAC AGATGTGTTG TATATGACCC GAATACAAAA GGAGAGATTT 
AATTCTACTG ACGAATACAA TGCTTGCTTT GGCCAGTTTA TCCTGACGCC GCACATCATG 
ACCAGGGCCA AGAAGAAAAT GGTGGTAATG CATCCGATGC CTCGTGTGAA TGAGATCAGC 
TTTGAAGTGG ATTCCGACCC TCGGGCAGCT TACTTTCGCC AGGCTGAGAA TGGCATGTAT 
ATCCGCATGG CTCTGCTAGC AACGGTTTTG GGAAAATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757791.1
CDS	111..6812
Translation	MAAGTAVLLLEDGTRVSGTVFGAVRPVSGEVVFQTGMVGYPEALTDPSYRAQILVLTYPLIGNYGIPQDELDQFGLSRWFESSKIHASALIIGERSRNPSHWSCDISLNQWLKQQGIPGLEGVDTRALTKKIREKGTLLGKLIPVGTEESQVPYEDPAKRNLVQEVSLKEPRVYNAGGTVRIAALDCGLKFNQIRCLCQRGAEVTVLPWDYPISSAECDGLFLSNGPGDPTFCQSSVRNIRDFISEEKPKPVFGICLGHQILSLVIGAHTYKMKYGNRGHNQPCIHESSRRCFITSQNHGYAVDPNTLPEGWSPLFTNANDQSNEGIIHDSKPFFSVQFHPEHCAGPMDLVGLFDVFLDTVRDRKAGDSGEKTVKQRVTELLTYKESPSVTDLEASRPHKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTVLINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGILLTFGGQTALNCGVELTKQGVLEKYNVRVLGTPVKSIEMTEDRKVFVEKMEEIGEHVAPSEAAFSLEQAQAAAERLGYPVLVRSAYALGGLGSGFASTKEELTALVCQAFAHTTQVLVDKSLKGWKEIEYEVVRDAYNNCVTVCNMENVDPLGIHTGESIVVAPSQTLNDREYNMLRNTAIKVIQHLGIVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPVLRNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMSVGRNFEEAFQKALRMVDENCVGFDHCIKSASDEELETPTDKRIFVLAAALRAGYTIERLYELTKIDRWFLHKMKNITDQVATLESYHQDETLMPREVLLRAKQLGFSDKQIALAVQSTELAVRKMRQELLILPVVKQIDTVAAEWPAQTNYLYLTYNGTEHDLAFEEPHVMVIGSGVYRIGSSVEFDWCAVGCIQELRKMGFKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGIILSMGGQLPNNIAMSLHRQQCRVLGTSPEAIDSAENRFKFSRMLDTIGISQPQWKELSDMESAVQFCNTVGFPCVVRPSYVLSGAAMNVAHSDGDLEKVLSNAVAVSKEHPVVISKFIQEAKEIDVDAVACDGVVVAVAISEHVENAGVHSGDATLVTPAQDITPKTLERIKAIVHAVGLELQVNGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVNLVALATQVIMGEEVEPVGVMTGTGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPQKNILLTIGSYKNKSELLPTVRAMESLGYSLYASLGTADFYTEHGIKVKAMDWPFEESENDGPLREKQGSIMDHLSENHFDLVINLSMRNSGGRRLSSFVTRGYRARRLAVDYSVPLIIDIKCTKLFVEALRQIGGAPPVKTHVDCMTSQKLIRLPGLVDIHVHLREPGATHKEDFSSGTAAALAGGITLVCAMPNTSPAITDASSFSLAQKLAEAGARCDFALFLGASSENAASLSSMASSAAGLKMYLNDTYSQLKMDNVSVWMEHFEKWPAHMPIVAHAEKQTVAAILMVAQLYQRPVHICHVAKKEEILIIKAAKQKGIQVTCEVAPHHLFLCLDDLKKIGETRGQVRPMLGTREDLESLWENLDIIDCFATDHAPHSVEEKDGPNPPPGYPGLETMLPLLLTAVSEGRLTIDDIIKRLYDNPQKIFSLPVQENTYVEVDLEQEWVIPSHMQFTKSRWSPFAGMKVKGAVRRVVLRGEVAYIDGQVLVPMGYGQDVRKWTPPVVAQSHPVAIKEIPKTPERHRHVTVQSETIRSRAPSPRRPCQSGDGRFHLPPRVHRASDPGFPELVPIQDGYSHPPPIPRIMSPQSTSMQSAPHVQTSPLLHPLIGQHILTVKQFSKDQMSHLFNVAHTLRMLVQKERNLDILKGKVMASMFFEASTRTSSSFAAAMQRLGGSVLSFCETTSSSQKGESLTDSVQTMSCYSDVLVVRHPEPGAVELAAKHCRKPVINAGDGVGEHPTQALLDIFTIREELGTVNGMIITMVGDLKHGRTVHSLACLLTQYRVTLRYVTPRSLRMPSNIIHFVASKGIKQEEFESIEEALPDTDVLYMTRIQKERFNSTDEYNACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISFEVDSDPRAAYFRQAENGMYIRMALLATVLGKY

Target ORF information:

RefSeq Version	XM_031901931.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901931.1

ORF Insert Sequence:

ATGGCTGCGG GTACTGCGGT GCTACTGCTG GAGGATGGAA CCCGGGTATC CGGAACCGTG 
TTCGGGGCGG TGCGTCCGGT GTCCGGGGAA GTGGTGTTCC AGACGGGCAT GGTGGGCTAC 
CCCGAGGCCC TCACCGACCC CTCCTACAGG GCCCAGATCC TGGTACTGAC ATATCCGCTG 
ATTGGGAACT ACGGGATCCC GCAGGATGAA CTGGACCAGT TTGGCCTCAG TCGGTGGTTC 
GAGTCCAGCA AGATTCACGC CTCGGCGCTG ATTATTGGGG AGCGGTCCCG GAACCCCAGT 
CACTGGAGCT GTGACATCTC ATTGAACCAG TGGCTCAAAC AGCAGGGAAT CCCCGGACTG 
GAAGGTGTGG ATACCCGGGC ACTCACCAAG AAGATCCGAG AGAAGGGCAC CCTGCTGGGC 
AAGCTCATAC CAGTGGGCAC AGAGGAGAGC CAGGTCCCTT ATGAAGATCC TGCCAAGAGG 
AACCTGGTTC AGGAGGTGTC ATTGAAGGAG CCCCGAGTGT ACAATGCGGG GGGCACAGTG 
AGAATCGCTG CCCTGGACTG TGGTTTGAAG TTCAACCAGA TTCGCTGCCT GTGCCAGAGG 
GGGGCCGAGG TGACGGTGCT GCCATGGGAC TATCCAATCA GCAGTGCAGA GTGTGACGGC 
CTGTTCCTCA GTAACGGCCC CGGGGACCCC ACGTTCTGTC AGAGCTCCGT GCGGAACATC 
AGGGACTTCA TTTCCGAGGA GAAGCCGAAA CCCGTGTTTG GGATCTGTCT GGGCCACCAG 
ATCCTCTCTC TGGTTATAGG CGCCCACACC TACAAGATGA AGTACGGGAA TCGTGGGCAC 
AACCAGCCGT GCATCCATGA GAGCTCTCGG CGCTGCTTCA TAACCTCACA GAACCATGGA 
TACGCCGTGG ACCCAAACAC TTTACCGGAA GGCTGGTCTC CTCTGTTCAC GAATGCCAAC 
GACCAATCAA ATGAGGGAAT TATCCATGAT TCTAAGCCCT TTTTCAGTGT GCAGTTCCAC 
CCCGAGCACT GTGCTGGGCC CATGGATCTG GTCGGCCTGT TTGATGTGTT CCTGGACACT 
GTGAGAGACC GGAAGGCTGG AGATTCAGGA GAGAAGACGG TTAAACAGCG AGTGACTGAG 
CTGCTCACAT ACAAGGAGTC TCCCAGTGTC ACAGACCTAG AGGCCTCTCG CCCACACAAA 
GTGCTGATCC TGGGCTCAGG GGGGCTGTCG ATTGGCCAGG CCGGGGAGTT CGACTACTCT 
GGCTCCCAGG CCATCAAAGC TCTGAAGGAA GAGAACATCC AGACTGTCCT GATTAACCCC 
AACATCGCCA CAGTGCAGAC ATCGAAAGGC CTCGCCGACA AGGTTTATTT CCTGCCAATC 
ACCCCTGAAT ATGTCACTCA GGTGATAAAG AATGAGCGGC CAGATGGGAT CCTCCTGACG 
TTTGGGGGTC AGACTGCATT GAACTGTGGG GTGGAGCTGA CCAAGCAGGG AGTCCTAGAG 
AAGTACAATG TGCGGGTTCT GGGGACCCCT GTGAAGTCCA TTGAAATGAC GGAAGATCGC 
AAAGTGTTTG TGGAGAAGAT GGAGGAGATT GGAGAACACG TGGCCCCCAG TGAGGCAGCT 
TTCTCCTTAG AGCAGGCCCA GGCTGCTGCA GAACGTCTCG GGTATCCGGT TTTGGTCCGG 
TCGGCCTACG CGCTGGGCGG CCTTGGATCT GGATTTGCCA GTACCAAGGA GGAGTTGACT 
GCCCTGGTGT GCCAGGCCTT CGCCCACACC ACACAGGTCC TGGTGGATAA ATCCCTGAAG 
GGCTGGAAGG AGATAGAGTA CGAAGTCGTG CGGGACGCCT ATAATAACTG TGTCACGGTG 
TGCAACATGG AGAACGTAGA TCCCCTGGGC ATTCACACTG GCGAGTCCAT CGTGGTAGCT 
CCCAGCCAGA CGCTGAACGA TCGAGAGTAC AACATGCTGC GCAACACGGC CATTAAGGTT 
ATCCAGCACC TCGGCATTGT GGGCGAGTGT AACATACAGT ACGCCCTAAA CCCGGAGTCT 
GAGCAGTATT ACATCATCGA GGTGAACGCA CGGCTGTCTC GCAGCTCAGC CCTGGCGAGT 
AAAGCAACTG GTTACCCCCT GGCGTATGTG GCTGCCAAGC TGGCACTGGG AATTCCTCTG 
CCCGTTCTCA GAAACTCCGT GACCAACTCC ACCACAGCCA ACTTTGAGCC CAGCCTGGAT 
TACTGTGTGG TGAAGGTTCC GCGCTGGGAC CTGAGCAAGT TCTTGCGAGT CAGCACCAAG 
ATTGGCAGCT CCATGAAGAG TGTGGGGGAG GTCATGTCTG TTGGTAGGAA CTTTGAAGAA 
GCCTTCCAGA AAGCTCTGCG CATGGTGGAC GAGAACTGCG TGGGCTTTGA TCACTGCATC 
AAATCCGCAT CTGATGAGGA ACTGGAGACG CCGACAGACA AGAGGATCTT CGTTCTGGCC 
GCTGCCCTGC GTGCAGGTTA CACTATCGAG CGCCTTTATG AGTTGACCAA GATTGACAGG 
TGGTTCCTCC ATAAGATGAA GAATATTACT GATCAGGTGG CCACGCTGGA GTCCTACCAC 
CAGGATGAGA CCTTGATGCC CCGTGAGGTC CTTCTCCGAG CCAAACAGCT GGGCTTCTCC 
GACAAGCAGA TTGCCCTGGC CGTGCAGAGC ACGGAGCTGG CAGTCCGCAA GATGCGGCAG 
GAGCTGCTGA TTTTGCCGGT AGTAAAGCAG ATTGACACGG TGGCGGCGGA GTGGCCGGCT 
CAGACCAATT ACCTGTATCT GACCTATAAT GGAACTGAGC ACGACTTGGC CTTTGAGGAG 
CCGCATGTTA TGGTGATTGG CTCTGGTGTT TACCGAATAG GCAGCAGTGT GGAGTTTGAT 
TGGTGCGCGG TGGGCTGCAT CCAGGAACTG CGTAAGATGG GATTTAAGAC CATCATGGTG 
AATTATAACC CCGAGACTGT CAGCACAGAC TATGACATGT GTGACCGCCT GTATTTTGAC 
GAGATCTCAT TTGAGGTGGT GATGGATATC TATGAGCTGG AGAACCCAGA GGGAATCATA 
CTGTCCATGG GTGGCCAGCT GCCCAATAAT ATTGCCATGT CCCTACATCG CCAGCAGTGC 
CGGGTGCTGG GCACTTCTCC TGAGGCCATC GACTCCGCTG AGAACAGGTT TAAGTTTTCT 
CGCATGTTGG ACACGATCGG AATCAGTCAG CCGCAGTGGA AAGAGCTGTC TGACATGGAG 
TCGGCCGTTC AGTTCTGTAA CACCGTCGGT TTCCCCTGCG TGGTGCGCCC ATCCTACGTG 
CTAAGTGGAG CCGCCATGAA TGTGGCCCAT TCAGATGGAG ACCTGGAGAA GGTGCTCAGT 
AACGCTGTGG CTGTGTCCAA GGAGCATCCC GTGGTCATCT CTAAATTCAT CCAGGAGGCC 
AAGGAAATTG ATGTGGATGC TGTGGCATGT GATGGAGTTG TTGTGGCCGT GGCTATTTCA 
GAACATGTGG AGAATGCTGG GGTTCACTCT GGGGATGCCA CACTGGTGAC CCCTGCTCAG 
GATATCACCC CCAAAACCTT GGAAAGGATT AAAGCCATTG TCCATGCTGT GGGGCTGGAG 
CTCCAAGTCA ACGGACCCTT TAACCTGCAG CTCATTGCAA AGGACGACCA GCTGAAAGTG 
ATCGAGTGCA ATGTCCGTGT GTCGCGCTCC TTTCCCTTTG TATCGAAGAC TCTGGGAGTC 
AATCTAGTCG CTCTGGCAAC GCAAGTCATT ATGGGAGAAG AGGTTGAACC TGTTGGTGTG 
ATGACTGGTA CTGGCATTGT AGGGGTTAAG GTCCCACAGT TCTCGTTCTC ACGCTTGGCC 
GGCGCGGACG TGGTCCTTGG GGTGGAGATG ACCAGCACCG GGGAGGTTGC CTGCTTTGGA 
GAGAACCGAT ACGAGGCCTA TCTAAAAGCC ATGCTAAGTA CCGGCTTCAA AATTCCACAG 
AAGAACATCC TACTGACCAT CGGGAGCTAC AAGAACAAGA GTGAGCTGCT GCCCACGGTG 
CGAGCCATGG AGAGTCTTGG CTACAGTCTG TATGCCAGCC TGGGAACTGC TGACTTCTAT 
ACGGAGCATG GCATCAAGGT AAAGGCAATG GATTGGCCCT TTGAGGAGAG TGAGAATGAC 
GGGCCCCTAC GGGAGAAGCA GGGCAGCATC ATGGACCATC TGTCAGAGAA TCACTTTGAT 
TTGGTCATTA ATTTGTCCAT GCGGAATTCT GGGGGGCGCC GACTCTCCTC CTTCGTTACC 
CGTGGGTACC GTGCCCGCCG CTTGGCTGTG GACTATTCTG TGCCCCTCAT CATTGATATC 
AAGTGCACCA AACTCTTTGT GGAGGCTCTG CGTCAGATTG GGGGGGCGCC CCCTGTGAAG 
ACTCATGTCG ACTGTATGAC CTCACAGAAA CTTATCAGGC TGCCAGGCCT AGTGGACATC 
CACGTGCACC TGCGGGAACC TGGCGCTACT CATAAGGAGG ACTTTTCTTC TGGCACAGCA 
GCTGCCCTGG CTGGTGGAAT TACCCTTGTA TGTGCCATGC CCAACACCAG CCCAGCCATC 
ACTGATGCCA GTTCCTTCTC TCTGGCACAG AAGCTGGCAG AGGCCGGTGC CCGCTGTGAC 
TTTGCCCTCT TCCTCGGTGC TTCCTCAGAG AATGCCGCTT CTCTTTCTTC GATGGCCAGT 
TCAGCCGCCG GCCTTAAGAT GTATCTGAAC GACACCTATT CGCAGCTTAA GATGGACAAT 
GTTTCTGTGT GGATGGAGCA CTTTGAGAAG TGGCCGGCAC ACATGCCCAT TGTGGCCCAT 
GCAGAGAAAC AGACCGTGGC GGCAATTCTC ATGGTGGCTC AGCTGTACCA GCGCCCTGTG 
CACATTTGCC ACGTTGCAAA AAAAGAGGAG ATCCTTATTA TCAAAGCAGC CAAGCAGAAG 
GGAATCCAAG TTACATGCGA GGTGGCCCCT CATCACCTTT TCCTGTGTCT CGATGATCTG 
AAGAAAATCG GGGAGACACG GGGGCAAGTA CGTCCCATGT TGGGGACTCG GGAGGATCTA 
GAATCTCTGT GGGAGAACTT GGATATAATA GACTGTTTTG CCACTGACCA CGCCCCTCAC 
TCTGTGGAGG AGAAGGACGG TCCAAACCCA CCTCCAGGGT ATCCCGGCTT GGAGACCATG 
CTGCCTCTGC TACTCACAGC AGTCAGTGAG GGACGTCTGA CTATTGATGA TATCATTAAG 
CGCCTGTACG ACAACCCCCA GAAGATCTTC TCTTTGCCTG TGCAGGAGAA CACCTATGTC 
GAGGTGGATT TGGAGCAAGA GTGGGTGATC CCGTCACACA TGCAGTTTAC CAAGTCCAGA 
TGGAGCCCCT TTGCTGGAAT GAAGGTGAAA GGAGCAGTGC GGCGAGTTGT TCTCCGTGGG 
GAGGTGGCCT ACATTGATGG GCAGGTACTG GTACCCATGG GATATGGACA GGATGTCAGG 
AAATGGACCC CACCTGTTGT TGCCCAATCT CACCCAGTCG CCATTAAAGA GATCCCTAAG 
ACCCCAGAGC GACATCGCCA TGTTACGGTA CAAAGTGAGA CGATTCGCAG TCGAGCACCA 
AGCCCCCGCA GACCCTGTCA GTCCGGAGAC GGGCGCTTCC ACTTGCCTCC TCGAGTGCAT 
CGTGCATCAG ATCCTGGCTT CCCAGAGCTG GTACCCATTC AGGATGGCTA TTCCCACCCT 
CCTCCCATCC CCAGGATTAT GTCTCCCCAG AGTACGTCCA TGCAAAGTGC CCCCCACGTG 
CAGACCTCAC CCCTGTTGCA CCCGTTGATT GGCCAACACA TCCTGACGGT TAAACAGTTC 
AGCAAAGACC AGATGTCGCA TCTGTTCAAC GTGGCGCACA CGCTGCGCAT GCTGGTGCAG 
AAGGAGAGGA ACCTTGATAT CCTAAAGGGC AAGGTGATGG CCTCCATGTT TTTTGAGGCA 
AGCACACGGA CAAGCAGCTC TTTTGCTGCA GCTATGCAAC GCCTTGGGGG CTCTGTGCTC 
AGTTTCTGTG AGACAACCTC CTCTTCTCAA AAAGGAGAGT CCCTCACCGA CTCTGTTCAG 
ACAATGAGCT GCTATTCGGA TGTCCTTGTG GTGAGACACC CAGAGCCTGG AGCTGTGGAG 
CTTGCTGCCA AGCACTGCCG GAAGCCTGTG ATAAATGCTG GGGATGGCGT CGGCGAGCAC 
CCCACTCAGG CCCTACTGGA CATTTTCACA ATCCGTGAAG AGCTGGGCAC AGTCAATGGA 
ATGATAATAA CGATGGTGGG AGATCTGAAA CATGGCCGGA CAGTTCATTC CCTCGCCTGC 
CTCTTAACAC AGTACCGTGT GACCTTGAGA TACGTGACTC CCCGCAGCCT CCGTATGCCC 
TCCAACATCA TACACTTTGT AGCCTCCAAG GGCATTAAGC AGGAAGAGTT TGAGAGCATT 
GAAGAGGCTC TGCCAGATAC AGATGTGTTG TATATGACCC GAATACAAAA GGAGAGATTT 
AATTCTACTG ACGAATACAA TGCTTGCTTT GGCCAGTTTA TCCTGACGCC GCACATCATG 
ACCAGGGCCA AGAAGAAAAT GGTGGTAATG CATCCGATGC CTCGTGTGAA TGAGATCAGC 
TTTGAAGTGG ATTCCGACCC TCGGGCAGCT TACTTTCGCC AGGCTGAGAA TGGCATGTAT 
ATCCGCATGG CTCTGCTAGC AACGGTTTTG GGAAAATACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cad ( XM_004920201.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004920258.2 Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), mRNA.

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CloneID	OXa30698
Clone ID Related Accession (Same CDS sequence)	XM_004920201.2
Accession Version	XM_004920201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	CAD protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016684019.1) and transcript sequence (BC135128.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004920201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 818 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTGGGCT ACCCCGAGGC CCTCACCGAC CCCTCCTACA GGGCCCAGAT CCTGGTACTG 
ACATATCCGC TGATTGGGAA CTACGGGATC CCGCAGGATG AACTGGACCA GTTTGGCCTC 
AGTCGGTGGT TCGAGTCCAG CAAGATTCAC GCCTCGGCGC TGATTATTGG GGAGCGGTCC 
CGGAACCCCA GTCACTGGAG CTGTGACATC TCATTGAACC AGTGGCTCAA ACAGCAGGGA 
ATCCCCGGAC TGGAAGGTGT GGATACCCGG GCACTCACCA AGAAGATCCG AGAGAAGGGC 
ACCCTGCTGG GCAAGCTCAT ACCAGTGGGC ACAGAGGAGA GCCAGGTCCC TTATGAAGAT 
CCTGCCAAGA GGAACCTGGT TCAGGAGGTG TCATTGAAGG AGCCCCGAGT GTACAATGCG 
GGGGGCACAG TGAGAATCGC TGCCCTGGAC TGTGGTTTGA AGTTCAACCA GATTCGCTGC 
CTGTGCCAGA GGGGGGCCGA GGTGACGGTG CTGCCATGGG ACTATCCAAT CAGCAGTGCA 
GAGTGTGACG GCCTGTTCCT CAGTAACGGC CCCGGGGACC CCACGTTCTG TCAGAGCTCC 
GTGCGGAACA TCAGGGACTT CATTTCCGAG GAGAAGCCGA AACCCGTGTT TGGGATCTGT 
CTGGGCCACC AGATCCTCTC TCTGGTTATA GGCGCCCACA CCTACAAGAT GAAGTACGGG 
AATCGTGGGC ACAACCAGCC GTGCATCCAT GAGAGCTCTC GGCGCTGCTT CATAACCTCA 
CAGAACCATG GATACGCCGT GGACCCAAAC ACTTTACCGG AAGGCTGGTC TCCTCTGTTC 
ACGAATGCCA ACGACCAATC AAATGAGGGA ATTATCCATG ATTCTAAGCC CTTTTTCAGT 
GTGCAGTTCC ACCCCGAGCA CTGTGCTGGG CCCATGGATC TGGTCGGCCT GTTTGATGTG 
TTCCTGGACA CTGTGAGAGA CCGGAAGGCT GGAGATTCAG GAGAGAAGAC GGTTAAACAG 
CGAGTGACTG AGCTGCTCAC ATACAAGGAG TCTCCCAGTG TCACAGACCT AGAGGCCTCT 
CGCCCACACA AAGTGCTGAT CCTGGGCTCA GGGGGGCTGT CGATTGGCCA GGCCGGGGAG 
TTCGACTACT CTGGCTCCCA GGCCATCAAA GCTCTGAAGG AAGAGAACAT CCAGACTGTC 
CTGATTAACC CCAACATCGC CACAGTGCAG ACATCGAAAG GCCTCGCCGA CAAGGTTTAT 
TTCCTGCCAA TCACCCCTGA ATATGTCACT CAGGTGATAA AGAATGAGCG GCCAGATGGG 
ATCCTCCTGA CGTTTGGGGG TCAGACTGCA TTGAACTGTG GGGTGGAGCT GACCAAGCAG 
GGAGTCCTAG AGAAGTACAA TGTGCGGGTT CTGGGGACCC CTGTGAAGTC CATTGAAATG 
ACGGAAGATC GCAAAGTGTT TGTGGAGAAG ATGGAGGAGA TTGGAGAACA CGTGGCCCCC 
AGTGAGGCAG CTTTCTCCTT AGAGCAGGCC CAGGCTGCTG CAGAACGTCT CGGGTATCCG 
GTTTTGGTCC GGTCGGCCTA CGCGCTGGGC GGCCTTGGAT CTGGATTTGC CAGTACCAAG 
GAGGAGTTGA CTGCCCTGGT GTGCCAGGCC TTCGCCCACA CCACACAGGT CCTGGTGGAT 
AAATCCCTGA AGGGCTGGAA GGAGATAGAG TACGAAGTCG TGCGGGACGC CTATAATAAC 
TGTGTCACGG TGTGCAACAT GGAGAACGTA GATCCCCTGG GCATTCACAC TGGCGAGTCC 
ATCGTGGTAG CTCCCAGCCA GACGCTGAAC GATCGAGAGT ACAACATGCT GCGCAACACG 
GCCATTAAGG TTATCCAGCA CCTCGGCATT GTGGGCGAGT GTAACATACA GTACGCCCTA 
AACCCGGAGT CTGAGCAGTA TTACATCATC GAGGTGAACG CACGGCTGTC TCGCAGCTCA 
GCCCTGGCGA GTAAAGCAAC TGGTTACCCC CTGGCGTATG TGGCTGCCAA GCTGGCACTG 
GGAATTCCTC TGCCCGTTCT CAGAAACTCC GTGACCAACT CCACCACAGC CAACTTTGAG 
CCCAGCCTGG ATTACTGTGT GGTGAAGGTT CCGCGCTGGG ACCTGAGCAA GTTCTTGCGA 
GTCAGCACCA AGATTGGCAG CTCCATGAAG AGTGTGGGGG AGGTCATGTC TGTTGGTAGG 
AACTTTGAAG AAGCCTTCCA GAAAGCTCTG CGCATGGTGG ACGAGAACTG CGTGGGCTTT 
GATCACTGCA TCAAATCCGC ATCTGATGAG GAACTGGAGA CGCCGACAGA CAAGAGGATC 
TTCGTTCTGG CCGCTGCCCT GCGTGCAGGT TACACTATCG AGCGCCTTTA TGAGTTGACC 
AAGATTGACA GGTGGTTCCT CCATAAGATG AAGAATATTA CTGATCAGGT GGCCACGCTG 
GAGTCCTACC ACCAGGATGA GACCTTGATG CCCCGTGAGG TCCTTCTCCG AGCCAAACAG 
CTGGGCTTCT CCGACAAGCA GATTGCCCTG GCCGTGCAGA GCACGGAGCT GGCAGTCCGC 
AAGATGCGGC AGGAGCTGCT GATTTTGCCG GTAGTAAAGC AGCTTGACAC GGTGGCGGCG 
GAGTGGCCGG CTCAGACCAA TTACCTGTAT CTGACCTATA ATGGAACTGA GCACGACTTG 
GCCTTTGAGG AGCCGCATGT TATGGTGATT GGCTCTGGTG TTTACCGAAT AGGCAGCAGT 
GTGGAGTTTG ATTGGTGCGC GGTGGGCTGC ATCCAGGAAC TGCGTAAGAT GGGATTTAAG 
ACCATCATGG TGAATTATAA CCCCGAGACT GTCAGCACAG ACTATGACAT GTGTGACCGC 
CTGTATTTTG ACGAGATCTC ATTTGAGGTG GTGATGGATA TCTATGAGCT GGAGAACCCA 
GAGGGAATCA TACTGTCCAT GGGTGGCCAG CTGCCCAATA ATATTGCCAT GTCCCTACAT 
CGCCAGCAGT GCCGGGTGCT GGGCACTTCT CCTGAGGCCA TCGACTCCGC TGAGAACAGG 
TTTAAGTTTT CTCGCATGTT GGACACGATC GGAATCAGTC AGCCGCAGTG GAAAGAGCTG 
TCTGACATGG AGTCGGCCGT TCAGTTCTGT AACACCGTCG GTTTCCCCTG CGTGGTGCGC 
CCATCCTACG TGCTAAGTGG AGCCGCCATG AATGTGGCCC ATTCAGATGG AGACCTGGAG 
AAGGTGCTCA GTAACGCTGT GGCTGTGTCC AAGGAGCATC CCGTGGTCAT CTCTAAATTC 
ATCCAGGAGG CCAAGGAAAT TGATGTGGAT GCTGTGGCAT GTGATGGAGT TGTTGTGGCC 
GTGGCTATTT CAGAACATGT GGAGAATGCT GGGGTTCACT CTGGGGATGC CACACTGGTG 
ACCCCTGCTC AGGATATCAC CCCCAAAACC TTGGAAAGGA TTAAAGCCAT TGTCCATGCT 
GTGGGGCTGG AGCTCCAAGT CAACGGACCC TTTAACCTGC AGCTCATTGC AAAGGACGAC 
CAGCTGAAAG TGATCGAGTG CAATGTCCGT GTGTCGCGCT CCTTTCCCTT TGTATCGAAG 
ACTCTGGGAG TCAATCTAGT CGCTCTGGCA ACGCAAGTCA TTATGGGAGA AGAGGTTGAA 
CCTGTTGGTG TGATGACTGG TACTGGCATT GTAGGGGTTA AGGTCCCACA GTTCTCGTTC 
TCACGCTTGG CCGGCGCGGA CGTGGTCCTT GGGGTGGAGA TGACCAGCAC CGGGGAGGTT 
GCCTGCTTTG GAGAGAACCG ATACGAGGCC TATCTAAAAG CCATGCTAAG TACCGGCTTC 
AAAATTCCAC AGAAGAACAT CCTACTGACC ATCGGGAGCT ACAAGAACAA GAGTGAGCTG 
CTGCCCACGG TGCGAGCCAT GGAGAGTCTT GGCTACAGTC TGTATGCCAG CCTGGGAACT 
GCTGACTTCT ATACGGAGCA TGGCATCAAG GTAAAGGCAA TGGATTGGCC CTTTGAGGAG 
AGTGAGAATG ACGGGCCCCT ACGGGAGAAG CAGGGCAGCA TCATGGACCA TCTGTCAGAG 
AATCACTTTG ATTTGGTCAT TAATTTGTCC ATGCGGAATT CTGGGGGGCG CCGACTCTCC 
TCCTTCGTTA CCCGTGGGTA CCGTGCCCGC CGCTTGGCTG TGGACTATTC TGTGCCCCTC 
ATCATTGATA TCAAGTGCAC CAAACTCTTT GTGGAGGCTC TGCGTCAGAT TGGGGGGGCG 
CCCCCTGTGA AGACTCATGT CGACTGTATG ACCTCACAGA AACTTATCAG GCTGCCAGGC 
CTAGTGGACA TCCACGTGCA CCTGCGGGAA CCTGGCGCTA CTCATAAGGA GGACTTTTCT 
TCTGGCACAG CAGCTGCCCT GGCTGGTGGA ATTACCCTTG TATGTGCCAT GCCCAACACC 
AGCCCAGCCA TCACTGATGC CAGTTCCTTC TCTCTGGCAC AGAAGCTGGC AGAGGCCGGT 
GCCCGCTGTG ACTTTGCCCT CTTCCTCGGT GCTTCCTCAG AGAATGCCGC TTCTCTTTCT 
TCGATGGCCA GTTCAGCCGC CGGCCTTAAG ATGTATCTGA ACGACACCTA TTCGCAGCTT 
AAGATGGACA ATGTTTCTGT GTGGATGGAG CACTTTGAGA AGTGGCCGGC ACACATGCCC 
ATTGTGGCCC ATGCAGAGAA ACAGACCGTG GCGGCAATTC TCATGGTGGC TCAGCTGTAC 
CAGCGCCCTG TGCACATTTG CCACGTTGCA AAAAAAGAGG AGATCCTTAT TATCAAAGCA 
GCCAAGCAGA AGGGAATCCA AGTTACATGC GAGGTGGCCC CTCATCACCT TTTCCTGTGT 
CTCGATGATC TGAAGAAAAT CGGGGAGACA CGGGGGCAAG TACGTCCCAT GTTGGGGACT 
CGGGAGGATC TAGAATCTCT GTGGGAGAAC TTGGATATAA TAGACTGTTT TGCCACTGAC 
CACGCCCCTC ACTCTGTGGA GGAGAAGGAC GGTCCAAACC CACCTCCAGG GTATCCCGGC 
TTGGAGACCA TGCTGCCTCT GCTACTCACA GCAGTCAGTG AGGGACGTCT GACTATTGAT 
GATATCATTA AGCGCCTGTA CGACAACCCC CAGAAGATCT TCTCTTTGCC TGTGCAGGAG 
AACACCTATG TCGAGGTGGA TTTGGAGCAA GAGTGGGTGA TCCCGTCACA CATGCAGTTT 
ACCAAGTCCA GATGGACCCC CTTTGCTGGA ATGAAGGTGA AAGGAGCAGT GCGGCGAGTT 
GTTCTCCGTG GGGAGGTGGC CTACATTGAT GGGCAGGTAC TGGTACCCAT GGGATATGGA 
CAGGATGTCA GGAAATGGAC CCCACCTGTT GTTGCCCAAT CTCACCCAGT CGCCATTAAA 
GAGATCCCTA AGACCCCAGA GCGACATCGC CATGTTACGG TACAAAGTGA GACGATTCGC 
AGTCGAGCAC CAAGCCCCCG CAGACCCTGT CAGTCCGGAG ACGGGCGCTT CCACTTGCCT 
CCTCGAGTGC ATCGTGCATC AGATCCTGGC TTCCCAGAGC TGGTACCCAT TCAGGATGGC 
TATTCCCACC CTCCTCCCAT CCCCAGGATT ATGTCTCCCC AGAGTACGTC CATGCAAAGT 
GCCCCCCACG TGCAGACCTC ACCCCTGTTG CACCCGTTGA TTGGCCAACA CATCCTGACG 
GTTAAACAGT TCAGCAAAGA CCAGATGTCG CATCTGTTCA ACGTGGCGCA CACGCTGCGC 
ATGCTGGTGC AGAAGGAGAG GAACCTTGAT ATCCTAAAGG GCAAGGTGAT GGCCTCCATG 
TTTTTTGAGG CAAGCACACG GACAAGCAGC TCTTTTGCTG CAGCTATGCA ACGCCTTGGG 
GGCTCTGTGC TCAGTTTCTG TGAGACAACC TCCTCTTCTC AAAAAGGAGA GTCCCTCACC 
GACTCTGTTC AGACAATGAG CTGCTATTCG GATGTCCTTG TGGTGAGACA CCCAGAGCCT 
GGAGCTGTGG AGCTTGCTGC CAAGCACTGC CGGAAGCCTG TGATAAATGC TGGGGATGGC 
GTCGGCGAGC ACCCCACTCA GGCCCTACTG GACATTTTCA CAATCCGTGA AGAGCTGGGC 
ACAGTCAATG GAATGATAAT AACGATGGTG GGAGATCTGA AACATGGCCG GACAGTTCAT 
TCCCTCGCCT GCCTCTTAAC ACAGTACCGT GTGACCTTGA GATACGTGAC TCCCCGCAGC 
CTCCGTATGC CCTCCAACAT CATACACTTT GTAGCCTCCA AGGGCATTAA GCAGGAAGAG 
TTTGAGAGCA TTGAAGAGGC TCTGCCAGAT ACAGATGTGT TGTATATGAC CCGAATACAA 
AAGGAGAGAT TTAATTCTAC TGACGAATAC AATGCTTGCT TTGGCCAGTT TATCCTGACG 
CCGCACATCA TGACCAGGGC CAAGAAGAAA ATGGTGGTAA TGCATCCGAT GCCTCGTGTG 
AATGAGATCA GCTTTGAAGT GGATTCCGAC CCTCGGGCAG CTTACTTTCG CCAGGCTGAG 
AATGGCATGT ATATCCGCAT GGCTCTGCTA GCAACGGTTT TGGGAAAATA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004920258.2
CDS	106..6699
Translation	MVGYPEALTDPSYRAQILVLTYPLIGNYGIPQDELDQFGLSRWFESSKIHASALIIGERSRNPSHWSCDISLNQWLKQQGIPGLEGVDTRALTKKIREKGTLLGKLIPVGTEESQVPYEDPAKRNLVQEVSLKEPRVYNAGGTVRIAALDCGLKFNQIRCLCQRGAEVTVLPWDYPISSAECDGLFLSNGPGDPTFCQSSVRNIRDFISEEKPKPVFGICLGHQILSLVIGAHTYKMKYGNRGHNQPCIHESSRRCFITSQNHGYAVDPNTLPEGWSPLFTNANDQSNEGIIHDSKPFFSVQFHPEHCAGPMDLVGLFDVFLDTVRDRKAGDSGEKTVKQRVTELLTYKESPSVTDLEASRPHKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTVLINPNIATVQTSKGLADKVYFLPITPEYVTQVIKNERPDGILLTFGGQTALNCGVELTKQGVLEKYNVRVLGTPVKSIEMTEDRKVFVEKMEEIGEHVAPSEAAFSLEQAQAAAERLGYPVLVRSAYALGGLGSGFASTKEELTALVCQAFAHTTQVLVDKSLKGWKEIEYEVVRDAYNNCVTVCNMENVDPLGIHTGESIVVAPSQTLNDREYNMLRNTAIKVIQHLGIVGECNIQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPVLRNSVTNSTTANFEPSLDYCVVKVPRWDLSKFLRVSTKIGSSMKSVGEVMSVGRNFEEAFQKALRMVDENCVGFDHCIKSASDEELETPTDKRIFVLAAALRAGYTIERLYELTKIDRWFLHKMKNITDQVATLESYHQDETLMPREVLLRAKQLGFSDKQIALAVQSTELAVRKMRQELLILPVVKQLDTVAAEWPAQTNYLYLTYNGTEHDLAFEEPHVMVIGSGVYRIGSSVEFDWCAVGCIQELRKMGFKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGIILSMGGQLPNNIAMSLHRQQCRVLGTSPEAIDSAENRFKFSRMLDTIGISQPQWKELSDMESAVQFCNTVGFPCVVRPSYVLSGAAMNVAHSDGDLEKVLSNAVAVSKEHPVVISKFIQEAKEIDVDAVACDGVVVAVAISEHVENAGVHSGDATLVTPAQDITPKTLERIKAIVHAVGLELQVNGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVNLVALATQVIMGEEVEPVGVMTGTGIVGVKVPQFSFSRLAGADVVLGVEMTSTGEVACFGENRYEAYLKAMLSTGFKIPQKNILLTIGSYKNKSELLPTVRAMESLGYSLYASLGTADFYTEHGIKVKAMDWPFEESENDGPLREKQGSIMDHLSENHFDLVINLSMRNSGGRRLSSFVTRGYRARRLAVDYSVPLIIDIKCTKLFVEALRQIGGAPPVKTHVDCMTSQKLIRLPGLVDIHVHLREPGATHKEDFSSGTAAALAGGITLVCAMPNTSPAITDASSFSLAQKLAEAGARCDFALFLGASSENAASLSSMASSAAGLKMYLNDTYSQLKMDNVSVWMEHFEKWPAHMPIVAHAEKQTVAAILMVAQLYQRPVHICHVAKKEEILIIKAAKQKGIQVTCEVAPHHLFLCLDDLKKIGETRGQVRPMLGTREDLESLWENLDIIDCFATDHAPHSVEEKDGPNPPPGYPGLETMLPLLLTAVSEGRLTIDDIIKRLYDNPQKIFSLPVQENTYVEVDLEQEWVIPSHMQFTKSRWTPFAGMKVKGAVRRVVLRGEVAYIDGQVLVPMGYGQDVRKWTPPVVAQSHPVAIKEIPKTPERHRHVTVQSETIRSRAPSPRRPCQSGDGRFHLPPRVHRASDPGFPELVPIQDGYSHPPPIPRIMSPQSTSMQSAPHVQTSPLLHPLIGQHILTVKQFSKDQMSHLFNVAHTLRMLVQKERNLDILKGKVMASMFFEASTRTSSSFAAAMQRLGGSVLSFCETTSSSQKGESLTDSVQTMSCYSDVLVVRHPEPGAVELAAKHCRKPVINAGDGVGEHPTQALLDIFTIREELGTVNGMIITMVGDLKHGRTVHSLACLLTQYRVTLRYVTPRSLRMPSNIIHFVASKGIKQEEFESIEEALPDTDVLYMTRIQKERFNSTDEYNACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISFEVDSDPRAAYFRQAENGMYIRMALLATVLGKY

Target ORF information:

RefSeq Version	XM_004920201.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (cad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004920201.2

ORF Insert Sequence:

ATGGTGGGCT ACCCCGAGGC CCTCACCGAC CCCTCCTACA GGGCCCAGAT CCTGGTACTG 
ACATATCCGC TGATTGGGAA CTACGGGATC CCGCAGGATG AACTGGACCA GTTTGGCCTC 
AGTCGGTGGT TCGAGTCCAG CAAGATTCAC GCCTCGGCGC TGATTATTGG GGAGCGGTCC 
CGGAACCCCA GTCACTGGAG CTGTGACATC TCATTGAACC AGTGGCTCAA ACAGCAGGGA 
ATCCCCGGAC TGGAAGGTGT GGATACCCGG GCACTCACCA AGAAGATCCG AGAGAAGGGC 
ACCCTGCTGG GCAAGCTCAT ACCAGTGGGC ACAGAGGAGA GCCAGGTCCC TTATGAAGAT 
CCTGCCAAGA GGAACCTGGT TCAGGAGGTG TCATTGAAGG AGCCCCGAGT GTACAATGCG 
GGGGGCACAG TGAGAATCGC TGCCCTGGAC TGTGGTTTGA AGTTCAACCA GATTCGCTGC 
CTGTGCCAGA GGGGGGCCGA GGTGACGGTG CTGCCATGGG ACTATCCAAT CAGCAGTGCA 
GAGTGTGACG GCCTGTTCCT CAGTAACGGC CCCGGGGACC CCACGTTCTG TCAGAGCTCC 
GTGCGGAACA TCAGGGACTT CATTTCCGAG GAGAAGCCGA AACCCGTGTT TGGGATCTGT 
CTGGGCCACC AGATCCTCTC TCTGGTTATA GGCGCCCACA CCTACAAGAT GAAGTACGGG 
AATCGTGGGC ACAACCAGCC GTGCATCCAT GAGAGCTCTC GGCGCTGCTT CATAACCTCA 
CAGAACCATG GATACGCCGT GGACCCAAAC ACTTTACCGG AAGGCTGGTC TCCTCTGTTC 
ACGAATGCCA ACGACCAATC AAATGAGGGA ATTATCCATG ATTCTAAGCC CTTTTTCAGT 
GTGCAGTTCC ACCCCGAGCA CTGTGCTGGG CCCATGGATC TGGTCGGCCT GTTTGATGTG 
TTCCTGGACA CTGTGAGAGA CCGGAAGGCT GGAGATTCAG GAGAGAAGAC GGTTAAACAG 
CGAGTGACTG AGCTGCTCAC ATACAAGGAG TCTCCCAGTG TCACAGACCT AGAGGCCTCT 
CGCCCACACA AAGTGCTGAT CCTGGGCTCA GGGGGGCTGT CGATTGGCCA GGCCGGGGAG 
TTCGACTACT CTGGCTCCCA GGCCATCAAA GCTCTGAAGG AAGAGAACAT CCAGACTGTC 
CTGATTAACC CCAACATCGC CACAGTGCAG ACATCGAAAG GCCTCGCCGA CAAGGTTTAT 
TTCCTGCCAA TCACCCCTGA ATATGTCACT CAGGTGATAA AGAATGAGCG GCCAGATGGG 
ATCCTCCTGA CGTTTGGGGG TCAGACTGCA TTGAACTGTG GGGTGGAGCT GACCAAGCAG 
GGAGTCCTAG AGAAGTACAA TGTGCGGGTT CTGGGGACCC CTGTGAAGTC CATTGAAATG 
ACGGAAGATC GCAAAGTGTT TGTGGAGAAG ATGGAGGAGA TTGGAGAACA CGTGGCCCCC 
AGTGAGGCAG CTTTCTCCTT AGAGCAGGCC CAGGCTGCTG CAGAACGTCT CGGGTATCCG 
GTTTTGGTCC GGTCGGCCTA CGCGCTGGGC GGCCTTGGAT CTGGATTTGC CAGTACCAAG 
GAGGAGTTGA CTGCCCTGGT GTGCCAGGCC TTCGCCCACA CCACACAGGT CCTGGTGGAT 
AAATCCCTGA AGGGCTGGAA GGAGATAGAG TACGAAGTCG TGCGGGACGC CTATAATAAC 
TGTGTCACGG TGTGCAACAT GGAGAACGTA GATCCCCTGG GCATTCACAC TGGCGAGTCC 
ATCGTGGTAG CTCCCAGCCA GACGCTGAAC GATCGAGAGT ACAACATGCT GCGCAACACG 
GCCATTAAGG TTATCCAGCA CCTCGGCATT GTGGGCGAGT GTAACATACA GTACGCCCTA 
AACCCGGAGT CTGAGCAGTA TTACATCATC GAGGTGAACG CACGGCTGTC TCGCAGCTCA 
GCCCTGGCGA GTAAAGCAAC TGGTTACCCC CTGGCGTATG TGGCTGCCAA GCTGGCACTG 
GGAATTCCTC TGCCCGTTCT CAGAAACTCC GTGACCAACT CCACCACAGC CAACTTTGAG 
CCCAGCCTGG ATTACTGTGT GGTGAAGGTT CCGCGCTGGG ACCTGAGCAA GTTCTTGCGA 
GTCAGCACCA AGATTGGCAG CTCCATGAAG AGTGTGGGGG AGGTCATGTC TGTTGGTAGG 
AACTTTGAAG AAGCCTTCCA GAAAGCTCTG CGCATGGTGG ACGAGAACTG CGTGGGCTTT 
GATCACTGCA TCAAATCCGC ATCTGATGAG GAACTGGAGA CGCCGACAGA CAAGAGGATC 
TTCGTTCTGG CCGCTGCCCT GCGTGCAGGT TACACTATCG AGCGCCTTTA TGAGTTGACC 
AAGATTGACA GGTGGTTCCT CCATAAGATG AAGAATATTA CTGATCAGGT GGCCACGCTG 
GAGTCCTACC ACCAGGATGA GACCTTGATG CCCCGTGAGG TCCTTCTCCG AGCCAAACAG 
CTGGGCTTCT CCGACAAGCA GATTGCCCTG GCCGTGCAGA GCACGGAGCT GGCAGTCCGC 
AAGATGCGGC AGGAGCTGCT GATTTTGCCG GTAGTAAAGC AGCTTGACAC GGTGGCGGCG 
GAGTGGCCGG CTCAGACCAA TTACCTGTAT CTGACCTATA ATGGAACTGA GCACGACTTG 
GCCTTTGAGG AGCCGCATGT TATGGTGATT GGCTCTGGTG TTTACCGAAT AGGCAGCAGT 
GTGGAGTTTG ATTGGTGCGC GGTGGGCTGC ATCCAGGAAC TGCGTAAGAT GGGATTTAAG 
ACCATCATGG TGAATTATAA CCCCGAGACT GTCAGCACAG ACTATGACAT GTGTGACCGC 
CTGTATTTTG ACGAGATCTC ATTTGAGGTG GTGATGGATA TCTATGAGCT GGAGAACCCA 
GAGGGAATCA TACTGTCCAT GGGTGGCCAG CTGCCCAATA ATATTGCCAT GTCCCTACAT 
CGCCAGCAGT GCCGGGTGCT GGGCACTTCT CCTGAGGCCA TCGACTCCGC TGAGAACAGG 
TTTAAGTTTT CTCGCATGTT GGACACGATC GGAATCAGTC AGCCGCAGTG GAAAGAGCTG 
TCTGACATGG AGTCGGCCGT TCAGTTCTGT AACACCGTCG GTTTCCCCTG CGTGGTGCGC 
CCATCCTACG TGCTAAGTGG AGCCGCCATG AATGTGGCCC ATTCAGATGG AGACCTGGAG 
AAGGTGCTCA GTAACGCTGT GGCTGTGTCC AAGGAGCATC CCGTGGTCAT CTCTAAATTC 
ATCCAGGAGG CCAAGGAAAT TGATGTGGAT GCTGTGGCAT GTGATGGAGT TGTTGTGGCC 
GTGGCTATTT CAGAACATGT GGAGAATGCT GGGGTTCACT CTGGGGATGC CACACTGGTG 
ACCCCTGCTC AGGATATCAC CCCCAAAACC TTGGAAAGGA TTAAAGCCAT TGTCCATGCT 
GTGGGGCTGG AGCTCCAAGT CAACGGACCC TTTAACCTGC AGCTCATTGC AAAGGACGAC 
CAGCTGAAAG TGATCGAGTG CAATGTCCGT GTGTCGCGCT CCTTTCCCTT TGTATCGAAG 
ACTCTGGGAG TCAATCTAGT CGCTCTGGCA ACGCAAGTCA TTATGGGAGA AGAGGTTGAA 
CCTGTTGGTG TGATGACTGG TACTGGCATT GTAGGGGTTA AGGTCCCACA GTTCTCGTTC 
TCACGCTTGG CCGGCGCGGA CGTGGTCCTT GGGGTGGAGA TGACCAGCAC CGGGGAGGTT 
GCCTGCTTTG GAGAGAACCG ATACGAGGCC TATCTAAAAG CCATGCTAAG TACCGGCTTC 
AAAATTCCAC AGAAGAACAT CCTACTGACC ATCGGGAGCT ACAAGAACAA GAGTGAGCTG 
CTGCCCACGG TGCGAGCCAT GGAGAGTCTT GGCTACAGTC TGTATGCCAG CCTGGGAACT 
GCTGACTTCT ATACGGAGCA TGGCATCAAG GTAAAGGCAA TGGATTGGCC CTTTGAGGAG 
AGTGAGAATG ACGGGCCCCT ACGGGAGAAG CAGGGCAGCA TCATGGACCA TCTGTCAGAG 
AATCACTTTG ATTTGGTCAT TAATTTGTCC ATGCGGAATT CTGGGGGGCG CCGACTCTCC 
TCCTTCGTTA CCCGTGGGTA CCGTGCCCGC CGCTTGGCTG TGGACTATTC TGTGCCCCTC 
ATCATTGATA TCAAGTGCAC CAAACTCTTT GTGGAGGCTC TGCGTCAGAT TGGGGGGGCG 
CCCCCTGTGA AGACTCATGT CGACTGTATG ACCTCACAGA AACTTATCAG GCTGCCAGGC 
CTAGTGGACA TCCACGTGCA CCTGCGGGAA CCTGGCGCTA CTCATAAGGA GGACTTTTCT 
TCTGGCACAG CAGCTGCCCT GGCTGGTGGA ATTACCCTTG TATGTGCCAT GCCCAACACC 
AGCCCAGCCA TCACTGATGC CAGTTCCTTC TCTCTGGCAC AGAAGCTGGC AGAGGCCGGT 
GCCCGCTGTG ACTTTGCCCT CTTCCTCGGT GCTTCCTCAG AGAATGCCGC TTCTCTTTCT 
TCGATGGCCA GTTCAGCCGC CGGCCTTAAG ATGTATCTGA ACGACACCTA TTCGCAGCTT 
AAGATGGACA ATGTTTCTGT GTGGATGGAG CACTTTGAGA AGTGGCCGGC ACACATGCCC 
ATTGTGGCCC ATGCAGAGAA ACAGACCGTG GCGGCAATTC TCATGGTGGC TCAGCTGTAC 
CAGCGCCCTG TGCACATTTG CCACGTTGCA AAAAAAGAGG AGATCCTTAT TATCAAAGCA 
GCCAAGCAGA AGGGAATCCA AGTTACATGC GAGGTGGCCC CTCATCACCT TTTCCTGTGT 
CTCGATGATC TGAAGAAAAT CGGGGAGACA CGGGGGCAAG TACGTCCCAT GTTGGGGACT 
CGGGAGGATC TAGAATCTCT GTGGGAGAAC TTGGATATAA TAGACTGTTT TGCCACTGAC 
CACGCCCCTC ACTCTGTGGA GGAGAAGGAC GGTCCAAACC CACCTCCAGG GTATCCCGGC 
TTGGAGACCA TGCTGCCTCT GCTACTCACA GCAGTCAGTG AGGGACGTCT GACTATTGAT 
GATATCATTA AGCGCCTGTA CGACAACCCC CAGAAGATCT TCTCTTTGCC TGTGCAGGAG 
AACACCTATG TCGAGGTGGA TTTGGAGCAA GAGTGGGTGA TCCCGTCACA CATGCAGTTT 
ACCAAGTCCA GATGGACCCC CTTTGCTGGA ATGAAGGTGA AAGGAGCAGT GCGGCGAGTT 
GTTCTCCGTG GGGAGGTGGC CTACATTGAT GGGCAGGTAC TGGTACCCAT GGGATATGGA 
CAGGATGTCA GGAAATGGAC CCCACCTGTT GTTGCCCAAT CTCACCCAGT CGCCATTAAA 
GAGATCCCTA AGACCCCAGA GCGACATCGC CATGTTACGG TACAAAGTGA GACGATTCGC 
AGTCGAGCAC CAAGCCCCCG CAGACCCTGT CAGTCCGGAG ACGGGCGCTT CCACTTGCCT 
CCTCGAGTGC ATCGTGCATC AGATCCTGGC TTCCCAGAGC TGGTACCCAT TCAGGATGGC 
TATTCCCACC CTCCTCCCAT CCCCAGGATT ATGTCTCCCC AGAGTACGTC CATGCAAAGT 
GCCCCCCACG TGCAGACCTC ACCCCTGTTG CACCCGTTGA TTGGCCAACA CATCCTGACG 
GTTAAACAGT TCAGCAAAGA CCAGATGTCG CATCTGTTCA ACGTGGCGCA CACGCTGCGC 
ATGCTGGTGC AGAAGGAGAG GAACCTTGAT ATCCTAAAGG GCAAGGTGAT GGCCTCCATG 
TTTTTTGAGG CAAGCACACG GACAAGCAGC TCTTTTGCTG CAGCTATGCA ACGCCTTGGG 
GGCTCTGTGC TCAGTTTCTG TGAGACAACC TCCTCTTCTC AAAAAGGAGA GTCCCTCACC 
GACTCTGTTC AGACAATGAG CTGCTATTCG GATGTCCTTG TGGTGAGACA CCCAGAGCCT 
GGAGCTGTGG AGCTTGCTGC CAAGCACTGC CGGAAGCCTG TGATAAATGC TGGGGATGGC 
GTCGGCGAGC ACCCCACTCA GGCCCTACTG GACATTTTCA CAATCCGTGA AGAGCTGGGC 
ACAGTCAATG GAATGATAAT AACGATGGTG GGAGATCTGA AACATGGCCG GACAGTTCAT 
TCCCTCGCCT GCCTCTTAAC ACAGTACCGT GTGACCTTGA GATACGTGAC TCCCCGCAGC 
CTCCGTATGC CCTCCAACAT CATACACTTT GTAGCCTCCA AGGGCATTAA GCAGGAAGAG 
TTTGAGAGCA TTGAAGAGGC TCTGCCAGAT ACAGATGTGT TGTATATGAC CCGAATACAA 
AAGGAGAGAT TTAATTCTAC TGACGAATAC AATGCTTGCT TTGGCCAGTT TATCCTGACG 
CCGCACATCA TGACCAGGGC CAAGAAGAAA ATGGTGGTAA TGCATCCGAT GCCTCGTGTG 
AATGAGATCA GCTTTGAAGT GGATTCCGAC CCTCGGGCAG CTTACTTTCG CCAGGCTGAG 
AATGGCATGT ATATCCGCAT GGCTCTGCTA GCAACGGTTT TGGGAAAATA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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cad cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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