CAD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAD
Entrez Gene ID	790
Full Name	carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Synonyms	CDG1Z,EIEE50,GATD4
General protein information	Preferred Names carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase Names CAD protein CAD trifunctional protein multifunctional protein CAD
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015].
Disorder MIM:	114010
Disorder Html:	Epileptic encephalopathy, early infantile, 50, 616457 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004341.4	NP_004332.2	CAD protein isoform 1
XM_006712101.2	XP_006712164.1	CAD protein isoform X2
XM_024453131.1	XP_024308899.1	CAD protein isoform X2
NM_004341.5	NP_004332.2	CAD protein isoform 1
XM_006712101.3	XP_006712164.1	CAD protein isoform X1
XM_005264555.3	XP_005264612.1	CAD protein isoform X1
NM_001306079.1	NP_001293008.1	CAD protein isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Charge neutralization in the active site of the catalytic trimer of aspartate transcarbamoylase promotes diverse structural changes.
Endrizzi JA, Beernink PT
Protein science : a publication of the Protein Society26(11)2221-2228(2017 Nov)

Structural Insight into the Core of CAD, the Multifunctional Protein Leading De Novo Pyrimidine Biosynthesis.
Moreno-Morcillo M, Grande-Garc?a A, Ruiz-Ramos A, Del Ca?o-Ochoa F, Boskovic J, Ram?n-Maiques S
Structure (London, England : 1993)25(6)912-923.e5(2017 Jun)

Tracking a CAD-ALK gene rearrangement in urine and blood of a colorectal cancer patient treated with an ALK inhibitor.
Siravegna G, Sartore-Bianchi A, Mussolin B, Cassingena A, Amatu A, Novara L, Buscarino M, Corti G, Crisafulli G, Bartolini A, Tosi F, Erlander M, Di Nicolantonio F, Siena S, Bardelli A
Annals of oncology : official journal of the European Society for Medical Oncology28(6)1302-1308(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Charge neutralization in the active site of the catalytic trimer of aspartate transcarbamoylase promotes diverse structural changes.
Title: Charge neutralization in the active site of the catalytic trimer of aspartate transcarbamoylase promotes diverse structural changes.

This study showed that CAD deficiency co-occurrence of anaemia, anisopoikilocytosis, global developmental delay, and seizures.
Title: CAD mutations and uridine-responsive epileptic encephalopathy.

Changes in glycosylation in caused by mutations in CAD.
Title: Biallelic mutations in CAD, impair de novo pyrimidine biosynthesis and decrease glycosylation precursors.

These results establish CAD as a downstream effector of Rheb and suggest a possible role of Rheb in regulating de novo pyrimidine nucleotide synthesis
Title: Rheb protein binds CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) protein in a GTP- and effector domain-dependent manner and influences its cellular localization and carbamoyl-phosphate synthetase (CPSase) activity.

Previous study found three metal ions in huDHOase active site; in the present study, the putative third metal binding site in type II enzymes, such as StDHOase and ScDHOase, was created and identified.
Title: Creation of a putative third metal binding site in type II dihydroorotases significantly enhances enzyme activity.

The following CAD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104976	XM_024453131.1 Latest version!	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu13057	NM_004341.5 Latest version!	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu35604	NM_001306079.1 Latest version!	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu35600	XM_005264555.3 Latest version!	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu35603	XM_006712101.3 Latest version!	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu13057	NM_004341.4	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu35603	XM_006712101.2	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAD ( XM_024453131.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308899.1 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.

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CloneID	OHu104976
Clone ID Related Accession (Same CDS sequence)	XM_024453131.1
Accession Version	XM_024453131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGCG TTGGTGAAGT CATGGGCATT GGGCGTTCAT TTGAGGAGGC CTTCCAGAAG 
GCCCTGCGCA TGGTGGATGA GAACTGTGTG GGCTTTGATC ACACAGTGAA ACCAGTCAGC 
GATATGGAGT TGGAGACTCC AACAGATAAG CGGATTTTTG TGGTGGCAGC TGCTTTGTGG 
GCTGGTTATT CAGTGGACCG CCTGTATGAG CTCACACGCA TCGACCGCTG GTTCCTGCAC 
CGAATGAAGC GTATCATCGC ACATGCCCAG CTGCTAGAAC AACACCGTGG ACAGCCTTTG 
CCGCCAGACC TGCTGCAACA GGCCAAGTGT CTTGGCTTCT CAGACAAACA GATTGCCCTT 
GCAGTTCTGA GCACAGAGCT GGCTGTTCGC AAGCTGCGTC AGGAACTGGG GATCTGTCCA 
GCAGTGAAAC AGATTGACAC AGTTGCAGCT GAGTGGCCAG CCCAGACAAA TTACCTATAC 
CTAACGTATT GGGGCACCAC CCATGACCTC ACCTTTCGAA CACCTCATGT CCTAGTCCTT 
GGCTCTGGCG TCTACCGTAT TGGCTCTAGC GTTGAATTTG ACTGGTGTGC TGTAGGCTGC 
ATCCAGCAGC TCCGAAAGAT GGGATATAAG ACCATCATGG TGAACTATAA CCCAGAGACA 
GTCAGCACCG ACTATGACAT GTGTGATCGA CTCTACTTTG ATGAGATCTC TTTTGAGGTG 
GTGATGGACA TCTATGAGCT CGAGAACCCT GAAGGTGTGA TCCTATCCAT GGGTGGACAG 
CTGCCCAACA ACATGGCCAT GGCGTTGCAT CGGCAGCAGT GCCGGGTGCT GGGCACCTCC 
CCTGAAGCCA TTGACTCGGC TGAGAACCGT TTCAAGTTTT CCCGGCTCCT TGACACCATT 
GGTATCAGCC AGCCTCAGTG GAGGGAGCTC AGTGACCTCG AGTCTGCTCG CCAATTCTGC 
CAGACCGTGG GGTACCCCTG TGTGGTGCGC CCCTCCTATG TGCTGAGCGG TGCTGCTATG 
AATGTGGCCT ACACGGATGG AGACCTGGAG CGCTTCCTGA GCAGCGCAGC AGCCGTCTCC 
AAAGAGCATC CCGTGGTCAT CTCCAAGTTC ATCCAGGAGG CTAAGGAGAT TGACGTGGAT 
GCCGTGGCCT CTGATGGTGT GGTGGCAGCC ATCGCCATCT CTGAGCATGT GGAGAATGCA 
GGTGTGCATT CAGGTGATGC GACGCTGGTG ACCCCCCCAC AAGATATCAC TGCCAAAACC 
CTGGAGCGGA TCAAAGCCAT TGTGCATGCT GTGGGCCAGG AGCTACAGGT CACAGGACCC 
TTCAATCTGC AGCTCATTGC CAAGGATGAC CAGCTGAAAG TTATTGAATG CAACGTACGT 
GTCTCTCGCT CCTTCCCCTT CGTTTCCAAG ACACTGGGTG TGGACCTAGT AGCCTTGGCC 
ACGCGGGTCA TCATGGGGGA AGAAGTGGAA CCTGTGGGGC TAATGACTGG TTCTGGAGTC 
GTGGGAGTAA AGGTGCCTCA GTTCTCCTTC TCCCGCTTGG CGGGTGCTGA CGTGGTGTTG 
GGTGTGGAAA TGACCAGTAC TGGGGAGGTG GCCGGCTTTG GGGAGAGCCG CTGTGAGGCA 
TACCTCAAGG CCATGCTAAG CACTGGCTTT AAGATCCCCA AGAAGAATAT CCTGCTGACC 
ATTGGCAGCT ATAAGAACAA AAGCGAGCTG CTCCCAACTG TGCGGCTACT GGAGAGCCTG 
GGCTACAGCC TCTATGCCAG TCTCGGCACA GCTGACTTCT ACACTGAGCA TGGCGTCAAG 
GTAACAGCTG TGGACTGGCA CTTTGAGGAG GCTGTGGATG GTGAGTGCCC ACCACAGCGG 
AGCATCCTGG AGCAGCTAGC TGAGAAAAAC TTTGAGCTGG TGATTAACCT GTCAATGCGT 
GGAGCTGGGG GCCGGCGTCT CTCTTCCTTT GTCACCAAGG GCTACCGCAC CCGACGCTTG 
GCCGCTGACT TCTCCGTGCC CCTAATCATC GATATCAAGT GCACCAAACT CTTTGTGGAG 
GCCCTAGGCC AGATCGGGCC AGCCCCTCCT TTGAAGGTGC ATGTTGACTG TATGACCTCC 
CAAAAGCTTG TGCGACTGCC GGGATTGATT GATGTCCATG TGCACCTGCG GGAACCAGGT 
GGGACACATA AGGAGGACTT TGCTTCAGGC ACAGCCGCTG CCCTGGCTGG GGGTATCACC 
ATGGTGTGTG CCATGCCTAA TACCCGGCCC CCCATCATTG ACGCCCCTGC TCTGGCCCTG 
GCCCAGAAGC TGGCAGAGGC TGGCGCCCGG TGCGACTTTG CGCTATTCCT TGGGGCCTCG 
TCTGAAAATG CAGGAACCTT GGGCACCGTG GCCGGGTCTG CAGCCGGGCT GAAGCTTTAC 
CTCAATGAGA CCTTCTCTGA GCTGCGGCTG GACAGCGTGG TCCAGTGGAT GGAGCATTTC 
GAGACATGGC CCTCCCACCT CCCCATTGTG GCTCACGCAG AGCAGCAAAC CGTGGCTGCT 
GTCCTCATGG TGGCTCAGCT CACTCAGCGC TCAGTGCACA TATGTCACGT GGCACGGAAG 
GAGGAGATCC TGCTAATTAA AGCTGCAAAG GCACGGGGCT TGCCAGTGAC CTGCGAGGTG 
GCTCCCCACC ACCTGTTCCT AAGCCATGAT GACCTGGAGC GCCTGGGGCC TGGGAAGGGG 
GAGGTCCGGC CTGAGCTTGG CTCCCGCCAG GATGTGGAAG CCCTGTGGGA GAACATGGCT 
GTCATCGACT GCTTTGCCTC AGACCATGCT CCCCATACCT TGGAGGAGAA GTGTGGGTCC 
AGGCCCCCAC CTGGGTTCCC AGGGTTAGAG ACCATGCTGC CACTACTCCT GACGGCTGTA 
AGCGAGGGCC GGCTCAGCCT GGACGACCTG CTGCAGCGAT TGCACCACAA TCCTCGGCGC 
ATCTTTCACC TGCCCCCGCA GGAGGACACC TATGTGGAGG TGGATCTGGA GCATGAGTGG 
ACAATTCCCA GCCACATGCC CTTCTCCAAG GCCCACTGGA CACCTTTTGA AGGGCAGAAA 
GTGAAGGGCA CCGTCCGCCG TGTGGTCCTG CGAGGGGAGG TTGCCTATAT CGATGGGCAG 
GTTCTGGTAC CCCCGGGCTA TGGACAGGAT GTACGGAAGT GGCCACAGGG GGCTGTTCCT 
CAGCTCCCAC CCTCAGCCCC TGCCACTAGT GAGATGACCA CGACACCTGA AAGACCCCGC 
CGTGGCATCC CAGGGCTTCC TGATGGCCGC TTCCATCTGC CGCCCCGAAT CCATCGAGCC 
TCCGACCCAG GTTTGCCAGC TGTGTTCCTC CGCCCAGGAG CTGGGATCCC ACGGGGCAGC 
AGAGCGTGGG CTGAGGAGCC AAAGGAGAAG TCCTCTCGGA AGGTAGCCGA GCCAGAGCTG 
ATGGGAACCC CTGATGGCAC CTGCTACCCT CCACCACCAG TACCGAGACA GGCATCTCCC 
CAGAACCTGG GGACCCCTGG CTTGCTGCAC CCCCAGACCT CACCCCTGCT GCACTCATTA 
GTGGGCCAAC ATATCCTGTC CGTCCAGCAG TTCACCAAGG ATCAGATGTC TCACCTGTTC 
AATGTGGCAC ACACACTGCG TATGATGGTG CAGAAGGAGC GGAGCCTCGA CATCCTGAAG 
GGGAAGGTCA TGGCCTCCAT GTTCTATGAA GTGAGCACAC GGACCAGCAG CTCCTTTGCA 
GCAGCCATGG CCCGGCTGGG AGGTGCTGTG CTCAGCTTCT CGGAAGCCAC ATCGTCCGTC 
CAGAAGGGCG AATCCCTGGC TGACTCCGTG CAGACCATGA GCTGCTATGC CGACGTCGTC 
GTGCTCCGGC ACCCCCAGCC TGGAGCAGTG GAGCTGGCCG CCAAGCACTG CCGGAGGCCA 
GTGATCAATG CTGGGGATGG GGTCGGAGAG CACCCCACCC AGGCCCTGCT GGACATCTTC 
ACCATCCGTG AGGAGCTGGG AACTGTCAAT GGCATGACGA TCACGATGGT GGGTGACCTG 
AAGCACGGAC GCACAGTACA TTCCCTGGCC TGCCTGCTCA CCCAGTATCG TGTCAGCCTG 
CGCTACGTGG CACCTCCCAG CCTGCGCATG CCACCCACTG TGCGGGCCTT CGTGGCCTCC 
CGCGGCACCA AGCAGGAGGA ATTCGAGAGC ATTGAGGAGG CGCTGCCTGA CACTGATGTG 
CTCTACATGA CTCGAATCCA GAAGGAACGA TTTGGCTCTA CCCAGGAGTA CGAAGCTTGC 
TTTGGTCAGT TCATCCTCAC TCCCCACATC ATGACCCGGG CCAAGAAGAA GATGGTGGTG 
ATGCACCCGA TGCCCCGTGT CAACGAGATA AGCGTGGAAG TGGACTCGGA TCCCCGCGCA 
GCCTACTTCC GCCAGGCTGA GAACGGCATG TACATCCGCA TGGCTCTGTT AGCCACCGTG 
CTGGGCCGTT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308899.1
CDS	827..5281
Translation	MKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAVFLRPGAGIPRGSRAWAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	XM_024453131.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453131.1

ORF Insert Sequence:

ATGAAGAGCG TTGGTGAAGT CATGGGCATT GGGCGTTCAT TTGAGGAGGC CTTCCAGAAG 
GCCCTGCGCA TGGTGGATGA GAACTGTGTG GGCTTTGATC ACACAGTGAA ACCAGTCAGC 
GATATGGAGT TGGAGACTCC AACAGATAAG CGGATTTTTG TGGTGGCAGC TGCTTTGTGG 
GCTGGTTATT CAGTGGACCG CCTGTATGAG CTCACACGCA TCGACCGCTG GTTCCTGCAC 
CGAATGAAGC GTATCATCGC ACATGCCCAG CTGCTAGAAC AACACCGTGG ACAGCCTTTG 
CCGCCAGACC TGCTGCAACA GGCCAAGTGT CTTGGCTTCT CAGACAAACA GATTGCCCTT 
GCAGTTCTGA GCACAGAGCT GGCTGTTCGC AAGCTGCGTC AGGAACTGGG GATCTGTCCA 
GCAGTGAAAC AGATTGACAC AGTTGCAGCT GAGTGGCCAG CCCAGACAAA TTACCTATAC 
CTAACGTATT GGGGCACCAC CCATGACCTC ACCTTTCGAA CACCTCATGT CCTAGTCCTT 
GGCTCTGGCG TCTACCGTAT TGGCTCTAGC GTTGAATTTG ACTGGTGTGC TGTAGGCTGC 
ATCCAGCAGC TCCGAAAGAT GGGATATAAG ACCATCATGG TGAACTATAA CCCAGAGACA 
GTCAGCACCG ACTATGACAT GTGTGATCGA CTCTACTTTG ATGAGATCTC TTTTGAGGTG 
GTGATGGACA TCTATGAGCT CGAGAACCCT GAAGGTGTGA TCCTATCCAT GGGTGGACAG 
CTGCCCAACA ACATGGCCAT GGCGTTGCAT CGGCAGCAGT GCCGGGTGCT GGGCACCTCC 
CCTGAAGCCA TTGACTCGGC TGAGAACCGT TTCAAGTTTT CCCGGCTCCT TGACACCATT 
GGTATCAGCC AGCCTCAGTG GAGGGAGCTC AGTGACCTCG AGTCTGCTCG CCAATTCTGC 
CAGACCGTGG GGTACCCCTG TGTGGTGCGC CCCTCCTATG TGCTGAGCGG TGCTGCTATG 
AATGTGGCCT ACACGGATGG AGACCTGGAG CGCTTCCTGA GCAGCGCAGC AGCCGTCTCC 
AAAGAGCATC CCGTGGTCAT CTCCAAGTTC ATCCAGGAGG CTAAGGAGAT TGACGTGGAT 
GCCGTGGCCT CTGATGGTGT GGTGGCAGCC ATCGCCATCT CTGAGCATGT GGAGAATGCA 
GGTGTGCATT CAGGTGATGC GACGCTGGTG ACCCCCCCAC AAGATATCAC TGCCAAAACC 
CTGGAGCGGA TCAAAGCCAT TGTGCATGCT GTGGGCCAGG AGCTACAGGT CACAGGACCC 
TTCAATCTGC AGCTCATTGC CAAGGATGAC CAGCTGAAAG TTATTGAATG CAACGTACGT 
GTCTCTCGCT CCTTCCCCTT CGTTTCCAAG ACACTGGGTG TGGACCTAGT AGCCTTGGCC 
ACGCGGGTCA TCATGGGGGA AGAAGTGGAA CCTGTGGGGC TAATGACTGG TTCTGGAGTC 
GTGGGAGTAA AGGTGCCTCA GTTCTCCTTC TCCCGCTTGG CGGGTGCTGA CGTGGTGTTG 
GGTGTGGAAA TGACCAGTAC TGGGGAGGTG GCCGGCTTTG GGGAGAGCCG CTGTGAGGCA 
TACCTCAAGG CCATGCTAAG CACTGGCTTT AAGATCCCCA AGAAGAATAT CCTGCTGACC 
ATTGGCAGCT ATAAGAACAA AAGCGAGCTG CTCCCAACTG TGCGGCTACT GGAGAGCCTG 
GGCTACAGCC TCTATGCCAG TCTCGGCACA GCTGACTTCT ACACTGAGCA TGGCGTCAAG 
GTAACAGCTG TGGACTGGCA CTTTGAGGAG GCTGTGGATG GTGAGTGCCC ACCACAGCGG 
AGCATCCTGG AGCAGCTAGC TGAGAAAAAC TTTGAGCTGG TGATTAACCT GTCAATGCGT 
GGAGCTGGGG GCCGGCGTCT CTCTTCCTTT GTCACCAAGG GCTACCGCAC CCGACGCTTG 
GCCGCTGACT TCTCCGTGCC CCTAATCATC GATATCAAGT GCACCAAACT CTTTGTGGAG 
GCCCTAGGCC AGATCGGGCC AGCCCCTCCT TTGAAGGTGC ATGTTGACTG TATGACCTCC 
CAAAAGCTTG TGCGACTGCC GGGATTGATT GATGTCCATG TGCACCTGCG GGAACCAGGT 
GGGACACATA AGGAGGACTT TGCTTCAGGC ACAGCCGCTG CCCTGGCTGG GGGTATCACC 
ATGGTGTGTG CCATGCCTAA TACCCGGCCC CCCATCATTG ACGCCCCTGC TCTGGCCCTG 
GCCCAGAAGC TGGCAGAGGC TGGCGCCCGG TGCGACTTTG CGCTATTCCT TGGGGCCTCG 
TCTGAAAATG CAGGAACCTT GGGCACCGTG GCCGGGTCTG CAGCCGGGCT GAAGCTTTAC 
CTCAATGAGA CCTTCTCTGA GCTGCGGCTG GACAGCGTGG TCCAGTGGAT GGAGCATTTC 
GAGACATGGC CCTCCCACCT CCCCATTGTG GCTCACGCAG AGCAGCAAAC CGTGGCTGCT 
GTCCTCATGG TGGCTCAGCT CACTCAGCGC TCAGTGCACA TATGTCACGT GGCACGGAAG 
GAGGAGATCC TGCTAATTAA AGCTGCAAAG GCACGGGGCT TGCCAGTGAC CTGCGAGGTG 
GCTCCCCACC ACCTGTTCCT AAGCCATGAT GACCTGGAGC GCCTGGGGCC TGGGAAGGGG 
GAGGTCCGGC CTGAGCTTGG CTCCCGCCAG GATGTGGAAG CCCTGTGGGA GAACATGGCT 
GTCATCGACT GCTTTGCCTC AGACCATGCT CCCCATACCT TGGAGGAGAA GTGTGGGTCC 
AGGCCCCCAC CTGGGTTCCC AGGGTTAGAG ACCATGCTGC CACTACTCCT GACGGCTGTA 
AGCGAGGGCC GGCTCAGCCT GGACGACCTG CTGCAGCGAT TGCACCACAA TCCTCGGCGC 
ATCTTTCACC TGCCCCCGCA GGAGGACACC TATGTGGAGG TGGATCTGGA GCATGAGTGG 
ACAATTCCCA GCCACATGCC CTTCTCCAAG GCCCACTGGA CACCTTTTGA AGGGCAGAAA 
GTGAAGGGCA CCGTCCGCCG TGTGGTCCTG CGAGGGGAGG TTGCCTATAT CGATGGGCAG 
GTTCTGGTAC CCCCGGGCTA TGGACAGGAT GTACGGAAGT GGCCACAGGG GGCTGTTCCT 
CAGCTCCCAC CCTCAGCCCC TGCCACTAGT GAGATGACCA CGACACCTGA AAGACCCCGC 
CGTGGCATCC CAGGGCTTCC TGATGGCCGC TTCCATCTGC CGCCCCGAAT CCATCGAGCC 
TCCGACCCAG GTTTGCCAGC TGTGTTCCTC CGCCCAGGAG CTGGGATCCC ACGGGGCAGC 
AGAGCGTGGG CTGAGGAGCC AAAGGAGAAG TCCTCTCGGA AGGTAGCCGA GCCAGAGCTG 
ATGGGAACCC CTGATGGCAC CTGCTACCCT CCACCACCAG TACCGAGACA GGCATCTCCC 
CAGAACCTGG GGACCCCTGG CTTGCTGCAC CCCCAGACCT CACCCCTGCT GCACTCATTA 
GTGGGCCAAC ATATCCTGTC CGTCCAGCAG TTCACCAAGG ATCAGATGTC TCACCTGTTC 
AATGTGGCAC ACACACTGCG TATGATGGTG CAGAAGGAGC GGAGCCTCGA CATCCTGAAG 
GGGAAGGTCA TGGCCTCCAT GTTCTATGAA GTGAGCACAC GGACCAGCAG CTCCTTTGCA 
GCAGCCATGG CCCGGCTGGG AGGTGCTGTG CTCAGCTTCT CGGAAGCCAC ATCGTCCGTC 
CAGAAGGGCG AATCCCTGGC TGACTCCGTG CAGACCATGA GCTGCTATGC CGACGTCGTC 
GTGCTCCGGC ACCCCCAGCC TGGAGCAGTG GAGCTGGCCG CCAAGCACTG CCGGAGGCCA 
GTGATCAATG CTGGGGATGG GGTCGGAGAG CACCCCACCC AGGCCCTGCT GGACATCTTC 
ACCATCCGTG AGGAGCTGGG AACTGTCAAT GGCATGACGA TCACGATGGT GGGTGACCTG 
AAGCACGGAC GCACAGTACA TTCCCTGGCC TGCCTGCTCA CCCAGTATCG TGTCAGCCTG 
CGCTACGTGG CACCTCCCAG CCTGCGCATG CCACCCACTG TGCGGGCCTT CGTGGCCTCC 
CGCGGCACCA AGCAGGAGGA ATTCGAGAGC ATTGAGGAGG CGCTGCCTGA CACTGATGTG 
CTCTACATGA CTCGAATCCA GAAGGAACGA TTTGGCTCTA CCCAGGAGTA CGAAGCTTGC 
TTTGGTCAGT TCATCCTCAC TCCCCACATC ATGACCCGGG CCAAGAAGAA GATGGTGGTG 
ATGCACCCGA TGCCCCGTGT CAACGAGATA AGCGTGGAAG TGGACTCGGA TCCCCGCGCA 
GCCTACTTCC GCCAGGCTGA GAACGGCATG TACATCCGCA TGGCTCTGTT AGCCACCGTG 
CTGGGCCGTT TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( NM_004341.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004332.2 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1, mRNA.

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CloneID	OHu13057
Clone ID Related Accession (Same CDS sequence)	NM_004341.4 , NM_004341.5
Accession Version	NM_004341.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	CAD protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC065510.1, D78586.1, BU541191.1, BM927624.1, BU558264.1 and BQ447516.1. This sequence is a reference standard in the RefSeqGene project. On Apr 14, 2015 this sequence version replaced NM_004341.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065510.1, D78586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGGTGTGTAA CATGGAGAAC 
TTGGACCCAC TGGGCATCCA CACTGGTGAG TCCATAGTGG TGGCCCCTAG CCAGACACTG 
AATGACAGGG AGTATCAGCT CCTGAGGCAG ACAGCTATCA AGGTGACCCA GCACCTGGGA 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCTG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCTCGCAGC TCTGCCCTGG CCAGTAAGGC CACAGGTTAT 
CCACTGGCTT ATGTGGCAGC CAAGCTAGCA TTGGGCATCC CTTTGCCTGA GCTCAGGAAC 
TCTGTGACAG GGGGTACAGC AGCCTTTGAA CCCAGCGTGG ATTATTGTGT GGTGAAGATT 
CCTCGATGGG ACCTTAGCAA GTTCCTGCGA GTCAGCACAA AGATTGGGAG CTGCATGAAG 
AGCGTTGGTG AAGTCATGGG CATTGGGCGT TCATTTGAGG AGGCCTTCCA GAAGGCCCTG 
CGCATGGTGG ATGAGAACTG TGTGGGCTTT GATCACACAG TGAAACCAGT CAGCGATATG 
GAGTTGGAGA CTCCAACAGA TAAGCGGATT TTTGTGGTGG CAGCTGCTTT GTGGGCTGGT 
TATTCAGTGG ACCGCCTGTA TGAGCTCACA CGCATCGACC GCTGGTTCCT GCACCGAATG 
AAGCGTATCA TCGCACATGC CCAGCTGCTA GAACAACACC GTGGACAGCC TTTGCCGCCA 
GACCTGCTGC AACAGGCCAA GTGTCTTGGC TTCTCAGACA AACAGATTGC CCTTGCAGTT 
CTGAGCACAG AGCTGGCTGT TCGCAAGCTG CGTCAGGAAC TGGGGATCTG TCCAGCAGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT ATACCTAACG 
TATTGGGGCA CCACCCATGA CCTCACCTTT CGAACACCTC ATGTCCTAGT CCTTGGCTCT 
GGCGTCTACC GTATTGGCTC TAGCGTTGAA TTTGACTGGT GTGCTGTAGG CTGCATCCAG 
CAGCTCCGAA AGATGGGATA TAAGACCATC ATGGTGAACT ATAACCCAGA GACAGTCAGC 
ACCGACTATG ACATGTGTGA TCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGCTCGAGAA CCCTGAAGGT GTGATCCTAT CCATGGGTGG ACAGCTGCCC 
AACAACATGG CCATGGCGTT GCATCGGCAG CAGTGCCGGG TGCTGGGCAC CTCCCCTGAA 
GCCATTGACT CGGCTGAGAA CCGTTTCAAG TTTTCCCGGC TCCTTGACAC CATTGGTATC 
AGCCAGCCTC AGTGGAGGGA GCTCAGTGAC CTCGAGTCTG CTCGCCAATT CTGCCAGACC 
GTGGGGTACC CCTGTGTGGT GCGCCCCTCC TATGTGCTGA GCGGTGCTGC TATGAATGTG 
GCCTACACGG ATGGAGACCT GGAGCGCTTC CTGAGCAGCG CAGCAGCCGT CTCCAAAGAG 
CATCCCGTGG TCATCTCCAA GTTCATCCAG GAGGCTAAGG AGATTGACGT GGATGCCGTG 
GCCTCTGATG GTGTGGTGGC AGCCATCGCC ATCTCTGAGC ATGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCGACGCT GGTGACCCCC CCACAAGATA TCACTGCCAA AACCCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAAGTTATTG AATGCAACGT ACGTGTCTCT 
CGCTCCTTCC CCTTCGTTTC CAAGACACTG GGTGTGGACC TAGTAGCCTT GGCCACGCGG 
GTCATCATGG GGGAAGAAGT GGAACCTGTG GGGCTAATGA CTGGTTCTGG AGTCGTGGGA 
GTAAAGGTGC CTCAGTTCTC CTTCTCCCGC TTGGCGGGTG CTGACGTGGT GTTGGGTGTG 
GAAATGACCA GTACTGGGGA GGTGGCCGGC TTTGGGGAGA GCCGCTGTGA GGCATACCTC 
AAGGCCATGC TAAGCACTGG CTTTAAGATC CCCAAGAAGA ATATCCTGCT GACCATTGGC 
AGCTATAAGA ACAAAAGCGA GCTGCTCCCA ACTGTGCGGC TACTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGTCTCGG CACAGCTGAC TTCTACACTG AGCATGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA GGAGGCTGTG GATGGTGAGT GCCCACCACA GCGGAGCATC 
CTGGAGCAGC TAGCTGAGAA AAACTTTGAG CTGGTGATTA ACCTGTCAAT GCGTGGAGCT 
GGGGGCCGGC GTCTCTCTTC CTTTGTCACC AAGGGCTACC GCACCCGACG CTTGGCCGCT 
GACTTCTCCG TGCCCCTAAT CATCGATATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAG GTGCATGTTG ACTGTATGAC CTCCCAAAAG 
CTTGTGCGAC TGCCGGGATT GATTGATGTC CATGTGCACC TGCGGGAACC AGGTGGGACA 
CATAAGGAGG ACTTTGCTTC AGGCACAGCC GCTGCCCTGG CTGGGGGTAT CACCATGGTG 
TGTGCCATGC CTAATACCCG GCCCCCCATC ATTGACGCCC CTGCTCTGGC CCTGGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGGTGCGAC TTTGCGCTAT TCCTTGGGGC CTCGTCTGAA 
AATGCAGGAA CCTTGGGCAC CGTGGCCGGG TCTGCAGCCG GGCTGAAGCT TTACCTCAAT 
GAGACCTTCT CTGAGCTGCG GCTGGACAGC GTGGTCCAGT GGATGGAGCA TTTCGAGACA 
TGGCCCTCCC ACCTCCCCAT TGTGGCTCAC GCAGAGCAGC AAACCGTGGC TGCTGTCCTC 
ATGGTGGCTC AGCTCACTCA GCGCTCAGTG CACATATGTC ACGTGGCACG GAAGGAGGAG 
ATCCTGCTAA TTAAAGCTGC AAAGGCACGG GGCTTGCCAG TGACCTGCGA GGTGGCTCCC 
CACCACCTGT TCCTAAGCCA TGATGACCTG GAGCGCCTGG GGCCTGGGAA GGGGGAGGTC 
CGGCCTGAGC TTGGCTCCCG CCAGGATGTG GAAGCCCTGT GGGAGAACAT GGCTGTCATC 
GACTGCTTTG CCTCAGACCA TGCTCCCCAT ACCTTGGAGG AGAAGTGTGG GTCCAGGCCC 
CCACCTGGGT TCCCAGGGTT AGAGACCATG CTGCCACTAC TCCTGACGGC TGTAAGCGAG 
GGCCGGCTCA GCCTGGACGA CCTGCTGCAG CGATTGCACC ACAATCCTCG GCGCATCTTT 
CACCTGCCCC CGCAGGAGGA CACCTATGTG GAGGTGGATC TGGAGCATGA GTGGACAATT 
CCCAGCCACA TGCCCTTCTC CAAGGCCCAC TGGACACCTT TTGAAGGGCA GAAAGTGAAG 
GGCACCGTCC GCCGTGTGGT CCTGCGAGGG GAGGTTGCCT ATATCGATGG GCAGGTTCTG 
GTACCCCCGG GCTATGGACA GGATGTACGG AAGTGGCCAC AGGGGGCTGT TCCTCAGCTC 
CCACCCTCAG CCCCTGCCAC TAGTGAGATG ACCACGACAC CTGAAAGACC CCGCCGTGGC 
ATCCCAGGGC TTCCTGATGG CCGCTTCCAT CTGCCGCCCC GAATCCATCG AGCCTCCGAC 
CCAGGTTTGC CAGCTGAGGA GCCAAAGGAG AAGTCCTCTC GGAAGGTAGC CGAGCCAGAG 
CTGATGGGAA CCCCTGATGG CACCTGCTAC CCTCCACCAC CAGTACCGAG ACAGGCATCT 
CCCCAGAACC TGGGGACCCC TGGCTTGCTG CACCCCCAGA CCTCACCCCT GCTGCACTCA 
TTAGTGGGCC AACATATCCT GTCCGTCCAG CAGTTCACCA AGGATCAGAT GTCTCACCTG 
TTCAATGTGG CACACACACT GCGTATGATG GTGCAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAT GAAGTGAGCA CACGGACCAG CAGCTCCTTT 
GCAGCAGCCA TGGCCCGGCT GGGAGGTGCT GTGCTCAGCT TCTCGGAAGC CACATCGTCC 
GTCCAGAAGG GCGAATCCCT GGCTGACTCC GTGCAGACCA TGAGCTGCTA TGCCGACGTC 
GTCGTGCTCC GGCACCCCCA GCCTGGAGCA GTGGAGCTGG CCGCCAAGCA CTGCCGGAGG 
CCAGTGATCA ATGCTGGGGA TGGGGTCGGA GAGCACCCCA CCCAGGCCCT GCTGGACATC 
TTCACCATCC GTGAGGAGCT GGGAACTGTC AATGGCATGA CGATCACGAT GGTGGGTGAC 
CTGAAGCACG GACGCACAGT ACATTCCCTG GCCTGCCTGC TCACCCAGTA TCGTGTCAGC 
CTGCGCTACG TGGCACCTCC CAGCCTGCGC ATGCCACCCA CTGTGCGGGC CTTCGTGGCC 
TCCCGCGGCA CCAAGCAGGA GGAATTCGAG AGCATTGAGG AGGCGCTGCC TGACACTGAT 
GTGCTCTACA TGACTCGAAT CCAGAAGGAA CGATTTGGCT CTACCCAGGA GTACGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCCCAC ATCATGACCC GGGCCAAGAA GAAGATGGTG 
GTGATGCACC CGATGCCCCG TGTCAACGAG ATAAGCGTGG AAGTGGACTC GGATCCCCGC 
GCAGCCTACT TCCGCCAGGC TGAGAACGGC ATGTACATCC GCATGGCTCT GTTAGCCACC 
GTGCTGGGCC GTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004332.2
CDS	163..6840
Translation	MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_004341.4
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004341.4

ORF Insert Sequence:

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGGTGTGTAA CATGGAGAAC 
TTGGACCCAC TGGGCATCCA CACTGGTGAG TCCATAGTGG TGGCCCCTAG CCAGACACTG 
AATGACAGGG AGTATCAGCT CCTGAGGCAG ACAGCTATCA AGGTGACCCA GCACCTGGGA 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCTG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCTCGCAGC TCTGCCCTGG CCAGTAAGGC CACAGGTTAT 
CCACTGGCTT ATGTGGCAGC CAAGCTAGCA TTGGGCATCC CTTTGCCTGA GCTCAGGAAC 
TCTGTGACAG GGGGTACAGC AGCCTTTGAA CCCAGCGTGG ATTATTGTGT GGTGAAGATT 
CCTCGATGGG ACCTTAGCAA GTTCCTGCGA GTCAGCACAA AGATTGGGAG CTGCATGAAG 
AGCGTTGGTG AAGTCATGGG CATTGGGCGT TCATTTGAGG AGGCCTTCCA GAAGGCCCTG 
CGCATGGTGG ATGAGAACTG TGTGGGCTTT GATCACACAG TGAAACCAGT CAGCGATATG 
GAGTTGGAGA CTCCAACAGA TAAGCGGATT TTTGTGGTGG CAGCTGCTTT GTGGGCTGGT 
TATTCAGTGG ACCGCCTGTA TGAGCTCACA CGCATCGACC GCTGGTTCCT GCACCGAATG 
AAGCGTATCA TCGCACATGC CCAGCTGCTA GAACAACACC GTGGACAGCC TTTGCCGCCA 
GACCTGCTGC AACAGGCCAA GTGTCTTGGC TTCTCAGACA AACAGATTGC CCTTGCAGTT 
CTGAGCACAG AGCTGGCTGT TCGCAAGCTG CGTCAGGAAC TGGGGATCTG TCCAGCAGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT ATACCTAACG 
TATTGGGGCA CCACCCATGA CCTCACCTTT CGAACACCTC ATGTCCTAGT CCTTGGCTCT 
GGCGTCTACC GTATTGGCTC TAGCGTTGAA TTTGACTGGT GTGCTGTAGG CTGCATCCAG 
CAGCTCCGAA AGATGGGATA TAAGACCATC ATGGTGAACT ATAACCCAGA GACAGTCAGC 
ACCGACTATG ACATGTGTGA TCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGCTCGAGAA CCCTGAAGGT GTGATCCTAT CCATGGGTGG ACAGCTGCCC 
AACAACATGG CCATGGCGTT GCATCGGCAG CAGTGCCGGG TGCTGGGCAC CTCCCCTGAA 
GCCATTGACT CGGCTGAGAA CCGTTTCAAG TTTTCCCGGC TCCTTGACAC CATTGGTATC 
AGCCAGCCTC AGTGGAGGGA GCTCAGTGAC CTCGAGTCTG CTCGCCAATT CTGCCAGACC 
GTGGGGTACC CCTGTGTGGT GCGCCCCTCC TATGTGCTGA GCGGTGCTGC TATGAATGTG 
GCCTACACGG ATGGAGACCT GGAGCGCTTC CTGAGCAGCG CAGCAGCCGT CTCCAAAGAG 
CATCCCGTGG TCATCTCCAA GTTCATCCAG GAGGCTAAGG AGATTGACGT GGATGCCGTG 
GCCTCTGATG GTGTGGTGGC AGCCATCGCC ATCTCTGAGC ATGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCGACGCT GGTGACCCCC CCACAAGATA TCACTGCCAA AACCCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAAGTTATTG AATGCAACGT ACGTGTCTCT 
CGCTCCTTCC CCTTCGTTTC CAAGACACTG GGTGTGGACC TAGTAGCCTT GGCCACGCGG 
GTCATCATGG GGGAAGAAGT GGAACCTGTG GGGCTAATGA CTGGTTCTGG AGTCGTGGGA 
GTAAAGGTGC CTCAGTTCTC CTTCTCCCGC TTGGCGGGTG CTGACGTGGT GTTGGGTGTG 
GAAATGACCA GTACTGGGGA GGTGGCCGGC TTTGGGGAGA GCCGCTGTGA GGCATACCTC 
AAGGCCATGC TAAGCACTGG CTTTAAGATC CCCAAGAAGA ATATCCTGCT GACCATTGGC 
AGCTATAAGA ACAAAAGCGA GCTGCTCCCA ACTGTGCGGC TACTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGTCTCGG CACAGCTGAC TTCTACACTG AGCATGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA GGAGGCTGTG GATGGTGAGT GCCCACCACA GCGGAGCATC 
CTGGAGCAGC TAGCTGAGAA AAACTTTGAG CTGGTGATTA ACCTGTCAAT GCGTGGAGCT 
GGGGGCCGGC GTCTCTCTTC CTTTGTCACC AAGGGCTACC GCACCCGACG CTTGGCCGCT 
GACTTCTCCG TGCCCCTAAT CATCGATATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAG GTGCATGTTG ACTGTATGAC CTCCCAAAAG 
CTTGTGCGAC TGCCGGGATT GATTGATGTC CATGTGCACC TGCGGGAACC AGGTGGGACA 
CATAAGGAGG ACTTTGCTTC AGGCACAGCC GCTGCCCTGG CTGGGGGTAT CACCATGGTG 
TGTGCCATGC CTAATACCCG GCCCCCCATC ATTGACGCCC CTGCTCTGGC CCTGGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGGTGCGAC TTTGCGCTAT TCCTTGGGGC CTCGTCTGAA 
AATGCAGGAA CCTTGGGCAC CGTGGCCGGG TCTGCAGCCG GGCTGAAGCT TTACCTCAAT 
GAGACCTTCT CTGAGCTGCG GCTGGACAGC GTGGTCCAGT GGATGGAGCA TTTCGAGACA 
TGGCCCTCCC ACCTCCCCAT TGTGGCTCAC GCAGAGCAGC AAACCGTGGC TGCTGTCCTC 
ATGGTGGCTC AGCTCACTCA GCGCTCAGTG CACATATGTC ACGTGGCACG GAAGGAGGAG 
ATCCTGCTAA TTAAAGCTGC AAAGGCACGG GGCTTGCCAG TGACCTGCGA GGTGGCTCCC 
CACCACCTGT TCCTAAGCCA TGATGACCTG GAGCGCCTGG GGCCTGGGAA GGGGGAGGTC 
CGGCCTGAGC TTGGCTCCCG CCAGGATGTG GAAGCCCTGT GGGAGAACAT GGCTGTCATC 
GACTGCTTTG CCTCAGACCA TGCTCCCCAT ACCTTGGAGG AGAAGTGTGG GTCCAGGCCC 
CCACCTGGGT TCCCAGGGTT AGAGACCATG CTGCCACTAC TCCTGACGGC TGTAAGCGAG 
GGCCGGCTCA GCCTGGACGA CCTGCTGCAG CGATTGCACC ACAATCCTCG GCGCATCTTT 
CACCTGCCCC CGCAGGAGGA CACCTATGTG GAGGTGGATC TGGAGCATGA GTGGACAATT 
CCCAGCCACA TGCCCTTCTC CAAGGCCCAC TGGACACCTT TTGAAGGGCA GAAAGTGAAG 
GGCACCGTCC GCCGTGTGGT CCTGCGAGGG GAGGTTGCCT ATATCGATGG GCAGGTTCTG 
GTACCCCCGG GCTATGGACA GGATGTACGG AAGTGGCCAC AGGGGGCTGT TCCTCAGCTC 
CCACCCTCAG CCCCTGCCAC TAGTGAGATG ACCACGACAC CTGAAAGACC CCGCCGTGGC 
ATCCCAGGGC TTCCTGATGG CCGCTTCCAT CTGCCGCCCC GAATCCATCG AGCCTCCGAC 
CCAGGTTTGC CAGCTGAGGA GCCAAAGGAG AAGTCCTCTC GGAAGGTAGC CGAGCCAGAG 
CTGATGGGAA CCCCTGATGG CACCTGCTAC CCTCCACCAC CAGTACCGAG ACAGGCATCT 
CCCCAGAACC TGGGGACCCC TGGCTTGCTG CACCCCCAGA CCTCACCCCT GCTGCACTCA 
TTAGTGGGCC AACATATCCT GTCCGTCCAG CAGTTCACCA AGGATCAGAT GTCTCACCTG 
TTCAATGTGG CACACACACT GCGTATGATG GTGCAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAT GAAGTGAGCA CACGGACCAG CAGCTCCTTT 
GCAGCAGCCA TGGCCCGGCT GGGAGGTGCT GTGCTCAGCT TCTCGGAAGC CACATCGTCC 
GTCCAGAAGG GCGAATCCCT GGCTGACTCC GTGCAGACCA TGAGCTGCTA TGCCGACGTC 
GTCGTGCTCC GGCACCCCCA GCCTGGAGCA GTGGAGCTGG CCGCCAAGCA CTGCCGGAGG 
CCAGTGATCA ATGCTGGGGA TGGGGTCGGA GAGCACCCCA CCCAGGCCCT GCTGGACATC 
TTCACCATCC GTGAGGAGCT GGGAACTGTC AATGGCATGA CGATCACGAT GGTGGGTGAC 
CTGAAGCACG GACGCACAGT ACATTCCCTG GCCTGCCTGC TCACCCAGTA TCGTGTCAGC 
CTGCGCTACG TGGCACCTCC CAGCCTGCGC ATGCCACCCA CTGTGCGGGC CTTCGTGGCC 
TCCCGCGGCA CCAAGCAGGA GGAATTCGAG AGCATTGAGG AGGCGCTGCC TGACACTGAT 
GTGCTCTACA TGACTCGAAT CCAGAAGGAA CGATTTGGCT CTACCCAGGA GTACGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCCCAC ATCATGACCC GGGCCAAGAA GAAGATGGTG 
GTGATGCACC CGATGCCCCG TGTCAACGAG ATAAGCGTGG AAGTGGACTC GGATCCCCGC 
GCAGCCTACT TCCGCCAGGC TGAGAACGGC ATGTACATCC GCATGGCTCT GTTAGCCACC 
GTGCTGGGCC GTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( NM_004341.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004332.2 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1, mRNA.

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CloneID	OHu13057
Clone ID Related Accession (Same CDS sequence)	NM_004341.4 , NM_004341.5
Accession Version	NM_004341.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	CAD protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013403.9, BC065510.1, D78586.1, BU541191.1, BM927624.1, BU558264.1, BQ447516.1 and AC013413.6. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004341.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC065510.1, D78586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264705.9/ ENSP00000264705.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGGTGTGTAA CATGGAGAAC 
TTGGACCCAC TGGGCATCCA CACTGGTGAG TCCATAGTGG TGGCCCCTAG CCAGACACTG 
AATGACAGGG AGTATCAGCT CCTGAGGCAG ACAGCTATCA AGGTGACCCA GCACCTGGGA 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCTG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCTCGCAGC TCTGCCCTGG CCAGTAAGGC CACAGGTTAT 
CCACTGGCTT ATGTGGCAGC CAAGCTAGCA TTGGGCATCC CTTTGCCTGA GCTCAGGAAC 
TCTGTGACAG GGGGTACAGC AGCCTTTGAA CCCAGCGTGG ATTATTGTGT GGTGAAGATT 
CCTCGATGGG ACCTTAGCAA GTTCCTGCGA GTCAGCACAA AGATTGGGAG CTGCATGAAG 
AGCGTTGGTG AAGTCATGGG CATTGGGCGT TCATTTGAGG AGGCCTTCCA GAAGGCCCTG 
CGCATGGTGG ATGAGAACTG TGTGGGCTTT GATCACACAG TGAAACCAGT CAGCGATATG 
GAGTTGGAGA CTCCAACAGA TAAGCGGATT TTTGTGGTGG CAGCTGCTTT GTGGGCTGGT 
TATTCAGTGG ACCGCCTGTA TGAGCTCACA CGCATCGACC GCTGGTTCCT GCACCGAATG 
AAGCGTATCA TCGCACATGC CCAGCTGCTA GAACAACACC GTGGACAGCC TTTGCCGCCA 
GACCTGCTGC AACAGGCCAA GTGTCTTGGC TTCTCAGACA AACAGATTGC CCTTGCAGTT 
CTGAGCACAG AGCTGGCTGT TCGCAAGCTG CGTCAGGAAC TGGGGATCTG TCCAGCAGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT ATACCTAACG 
TATTGGGGCA CCACCCATGA CCTCACCTTT CGAACACCTC ATGTCCTAGT CCTTGGCTCT 
GGCGTCTACC GTATTGGCTC TAGCGTTGAA TTTGACTGGT GTGCTGTAGG CTGCATCCAG 
CAGCTCCGAA AGATGGGATA TAAGACCATC ATGGTGAACT ATAACCCAGA GACAGTCAGC 
ACCGACTATG ACATGTGTGA TCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGCTCGAGAA CCCTGAAGGT GTGATCCTAT CCATGGGTGG ACAGCTGCCC 
AACAACATGG CCATGGCGTT GCATCGGCAG CAGTGCCGGG TGCTGGGCAC CTCCCCTGAA 
GCCATTGACT CGGCTGAGAA CCGTTTCAAG TTTTCCCGGC TCCTTGACAC CATTGGTATC 
AGCCAGCCTC AGTGGAGGGA GCTCAGTGAC CTCGAGTCTG CTCGCCAATT CTGCCAGACC 
GTGGGGTACC CCTGTGTGGT GCGCCCCTCC TATGTGCTGA GCGGTGCTGC TATGAATGTG 
GCCTACACGG ATGGAGACCT GGAGCGCTTC CTGAGCAGCG CAGCAGCCGT CTCCAAAGAG 
CATCCCGTGG TCATCTCCAA GTTCATCCAG GAGGCTAAGG AGATTGACGT GGATGCCGTG 
GCCTCTGATG GTGTGGTGGC AGCCATCGCC ATCTCTGAGC ATGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCGACGCT GGTGACCCCC CCACAAGATA TCACTGCCAA AACCCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAAGTTATTG AATGCAACGT ACGTGTCTCT 
CGCTCCTTCC CCTTCGTTTC CAAGACACTG GGTGTGGACC TAGTAGCCTT GGCCACGCGG 
GTCATCATGG GGGAAGAAGT GGAACCTGTG GGGCTAATGA CTGGTTCTGG AGTCGTGGGA 
GTAAAGGTGC CTCAGTTCTC CTTCTCCCGC TTGGCGGGTG CTGACGTGGT GTTGGGTGTG 
GAAATGACCA GTACTGGGGA GGTGGCCGGC TTTGGGGAGA GCCGCTGTGA GGCATACCTC 
AAGGCCATGC TAAGCACTGG CTTTAAGATC CCCAAGAAGA ATATCCTGCT GACCATTGGC 
AGCTATAAGA ACAAAAGCGA GCTGCTCCCA ACTGTGCGGC TACTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGTCTCGG CACAGCTGAC TTCTACACTG AGCATGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA GGAGGCTGTG GATGGTGAGT GCCCACCACA GCGGAGCATC 
CTGGAGCAGC TAGCTGAGAA AAACTTTGAG CTGGTGATTA ACCTGTCAAT GCGTGGAGCT 
GGGGGCCGGC GTCTCTCTTC CTTTGTCACC AAGGGCTACC GCACCCGACG CTTGGCCGCT 
GACTTCTCCG TGCCCCTAAT CATCGATATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAG GTGCATGTTG ACTGTATGAC CTCCCAAAAG 
CTTGTGCGAC TGCCGGGATT GATTGATGTC CATGTGCACC TGCGGGAACC AGGTGGGACA 
CATAAGGAGG ACTTTGCTTC AGGCACAGCC GCTGCCCTGG CTGGGGGTAT CACCATGGTG 
TGTGCCATGC CTAATACCCG GCCCCCCATC ATTGACGCCC CTGCTCTGGC CCTGGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGGTGCGAC TTTGCGCTAT TCCTTGGGGC CTCGTCTGAA 
AATGCAGGAA CCTTGGGCAC CGTGGCCGGG TCTGCAGCCG GGCTGAAGCT TTACCTCAAT 
GAGACCTTCT CTGAGCTGCG GCTGGACAGC GTGGTCCAGT GGATGGAGCA TTTCGAGACA 
TGGCCCTCCC ACCTCCCCAT TGTGGCTCAC GCAGAGCAGC AAACCGTGGC TGCTGTCCTC 
ATGGTGGCTC AGCTCACTCA GCGCTCAGTG CACATATGTC ACGTGGCACG GAAGGAGGAG 
ATCCTGCTAA TTAAAGCTGC AAAGGCACGG GGCTTGCCAG TGACCTGCGA GGTGGCTCCC 
CACCACCTGT TCCTAAGCCA TGATGACCTG GAGCGCCTGG GGCCTGGGAA GGGGGAGGTC 
CGGCCTGAGC TTGGCTCCCG CCAGGATGTG GAAGCCCTGT GGGAGAACAT GGCTGTCATC 
GACTGCTTTG CCTCAGACCA TGCTCCCCAT ACCTTGGAGG AGAAGTGTGG GTCCAGGCCC 
CCACCTGGGT TCCCAGGGTT AGAGACCATG CTGCCACTAC TCCTGACGGC TGTAAGCGAG 
GGCCGGCTCA GCCTGGACGA CCTGCTGCAG CGATTGCACC ACAATCCTCG GCGCATCTTT 
CACCTGCCCC CGCAGGAGGA CACCTATGTG GAGGTGGATC TGGAGCATGA GTGGACAATT 
CCCAGCCACA TGCCCTTCTC CAAGGCCCAC TGGACACCTT TTGAAGGGCA GAAAGTGAAG 
GGCACCGTCC GCCGTGTGGT CCTGCGAGGG GAGGTTGCCT ATATCGATGG GCAGGTTCTG 
GTACCCCCGG GCTATGGACA GGATGTACGG AAGTGGCCAC AGGGGGCTGT TCCTCAGCTC 
CCACCCTCAG CCCCTGCCAC TAGTGAGATG ACCACGACAC CTGAAAGACC CCGCCGTGGC 
ATCCCAGGGC TTCCTGATGG CCGCTTCCAT CTGCCGCCCC GAATCCATCG AGCCTCCGAC 
CCAGGTTTGC CAGCTGAGGA GCCAAAGGAG AAGTCCTCTC GGAAGGTAGC CGAGCCAGAG 
CTGATGGGAA CCCCTGATGG CACCTGCTAC CCTCCACCAC CAGTACCGAG ACAGGCATCT 
CCCCAGAACC TGGGGACCCC TGGCTTGCTG CACCCCCAGA CCTCACCCCT GCTGCACTCA 
TTAGTGGGCC AACATATCCT GTCCGTCCAG CAGTTCACCA AGGATCAGAT GTCTCACCTG 
TTCAATGTGG CACACACACT GCGTATGATG GTGCAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAT GAAGTGAGCA CACGGACCAG CAGCTCCTTT 
GCAGCAGCCA TGGCCCGGCT GGGAGGTGCT GTGCTCAGCT TCTCGGAAGC CACATCGTCC 
GTCCAGAAGG GCGAATCCCT GGCTGACTCC GTGCAGACCA TGAGCTGCTA TGCCGACGTC 
GTCGTGCTCC GGCACCCCCA GCCTGGAGCA GTGGAGCTGG CCGCCAAGCA CTGCCGGAGG 
CCAGTGATCA ATGCTGGGGA TGGGGTCGGA GAGCACCCCA CCCAGGCCCT GCTGGACATC 
TTCACCATCC GTGAGGAGCT GGGAACTGTC AATGGCATGA CGATCACGAT GGTGGGTGAC 
CTGAAGCACG GACGCACAGT ACATTCCCTG GCCTGCCTGC TCACCCAGTA TCGTGTCAGC 
CTGCGCTACG TGGCACCTCC CAGCCTGCGC ATGCCACCCA CTGTGCGGGC CTTCGTGGCC 
TCCCGCGGCA CCAAGCAGGA GGAATTCGAG AGCATTGAGG AGGCGCTGCC TGACACTGAT 
GTGCTCTACA TGACTCGAAT CCAGAAGGAA CGATTTGGCT CTACCCAGGA GTACGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCCCAC ATCATGACCC GGGCCAAGAA GAAGATGGTG 
GTGATGCACC CGATGCCCCG TGTCAACGAG ATAAGCGTGG AAGTGGACTC GGATCCCCGC 
GCAGCCTACT TCCGCCAGGC TGAGAACGGC ATGTACATCC GCATGGCTCT GTTAGCCACC 
GTGCTGGGCC GTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004332.2
CDS	184..6861
Translation	MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_004341.5
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004341.5

ORF Insert Sequence:

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGGTGTGTAA CATGGAGAAC 
TTGGACCCAC TGGGCATCCA CACTGGTGAG TCCATAGTGG TGGCCCCTAG CCAGACACTG 
AATGACAGGG AGTATCAGCT CCTGAGGCAG ACAGCTATCA AGGTGACCCA GCACCTGGGA 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCTG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCTCGCAGC TCTGCCCTGG CCAGTAAGGC CACAGGTTAT 
CCACTGGCTT ATGTGGCAGC CAAGCTAGCA TTGGGCATCC CTTTGCCTGA GCTCAGGAAC 
TCTGTGACAG GGGGTACAGC AGCCTTTGAA CCCAGCGTGG ATTATTGTGT GGTGAAGATT 
CCTCGATGGG ACCTTAGCAA GTTCCTGCGA GTCAGCACAA AGATTGGGAG CTGCATGAAG 
AGCGTTGGTG AAGTCATGGG CATTGGGCGT TCATTTGAGG AGGCCTTCCA GAAGGCCCTG 
CGCATGGTGG ATGAGAACTG TGTGGGCTTT GATCACACAG TGAAACCAGT CAGCGATATG 
GAGTTGGAGA CTCCAACAGA TAAGCGGATT TTTGTGGTGG CAGCTGCTTT GTGGGCTGGT 
TATTCAGTGG ACCGCCTGTA TGAGCTCACA CGCATCGACC GCTGGTTCCT GCACCGAATG 
AAGCGTATCA TCGCACATGC CCAGCTGCTA GAACAACACC GTGGACAGCC TTTGCCGCCA 
GACCTGCTGC AACAGGCCAA GTGTCTTGGC TTCTCAGACA AACAGATTGC CCTTGCAGTT 
CTGAGCACAG AGCTGGCTGT TCGCAAGCTG CGTCAGGAAC TGGGGATCTG TCCAGCAGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT ATACCTAACG 
TATTGGGGCA CCACCCATGA CCTCACCTTT CGAACACCTC ATGTCCTAGT CCTTGGCTCT 
GGCGTCTACC GTATTGGCTC TAGCGTTGAA TTTGACTGGT GTGCTGTAGG CTGCATCCAG 
CAGCTCCGAA AGATGGGATA TAAGACCATC ATGGTGAACT ATAACCCAGA GACAGTCAGC 
ACCGACTATG ACATGTGTGA TCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGCTCGAGAA CCCTGAAGGT GTGATCCTAT CCATGGGTGG ACAGCTGCCC 
AACAACATGG CCATGGCGTT GCATCGGCAG CAGTGCCGGG TGCTGGGCAC CTCCCCTGAA 
GCCATTGACT CGGCTGAGAA CCGTTTCAAG TTTTCCCGGC TCCTTGACAC CATTGGTATC 
AGCCAGCCTC AGTGGAGGGA GCTCAGTGAC CTCGAGTCTG CTCGCCAATT CTGCCAGACC 
GTGGGGTACC CCTGTGTGGT GCGCCCCTCC TATGTGCTGA GCGGTGCTGC TATGAATGTG 
GCCTACACGG ATGGAGACCT GGAGCGCTTC CTGAGCAGCG CAGCAGCCGT CTCCAAAGAG 
CATCCCGTGG TCATCTCCAA GTTCATCCAG GAGGCTAAGG AGATTGACGT GGATGCCGTG 
GCCTCTGATG GTGTGGTGGC AGCCATCGCC ATCTCTGAGC ATGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCGACGCT GGTGACCCCC CCACAAGATA TCACTGCCAA AACCCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAAGTTATTG AATGCAACGT ACGTGTCTCT 
CGCTCCTTCC CCTTCGTTTC CAAGACACTG GGTGTGGACC TAGTAGCCTT GGCCACGCGG 
GTCATCATGG GGGAAGAAGT GGAACCTGTG GGGCTAATGA CTGGTTCTGG AGTCGTGGGA 
GTAAAGGTGC CTCAGTTCTC CTTCTCCCGC TTGGCGGGTG CTGACGTGGT GTTGGGTGTG 
GAAATGACCA GTACTGGGGA GGTGGCCGGC TTTGGGGAGA GCCGCTGTGA GGCATACCTC 
AAGGCCATGC TAAGCACTGG CTTTAAGATC CCCAAGAAGA ATATCCTGCT GACCATTGGC 
AGCTATAAGA ACAAAAGCGA GCTGCTCCCA ACTGTGCGGC TACTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGTCTCGG CACAGCTGAC TTCTACACTG AGCATGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA GGAGGCTGTG GATGGTGAGT GCCCACCACA GCGGAGCATC 
CTGGAGCAGC TAGCTGAGAA AAACTTTGAG CTGGTGATTA ACCTGTCAAT GCGTGGAGCT 
GGGGGCCGGC GTCTCTCTTC CTTTGTCACC AAGGGCTACC GCACCCGACG CTTGGCCGCT 
GACTTCTCCG TGCCCCTAAT CATCGATATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAG GTGCATGTTG ACTGTATGAC CTCCCAAAAG 
CTTGTGCGAC TGCCGGGATT GATTGATGTC CATGTGCACC TGCGGGAACC AGGTGGGACA 
CATAAGGAGG ACTTTGCTTC AGGCACAGCC GCTGCCCTGG CTGGGGGTAT CACCATGGTG 
TGTGCCATGC CTAATACCCG GCCCCCCATC ATTGACGCCC CTGCTCTGGC CCTGGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGGTGCGAC TTTGCGCTAT TCCTTGGGGC CTCGTCTGAA 
AATGCAGGAA CCTTGGGCAC CGTGGCCGGG TCTGCAGCCG GGCTGAAGCT TTACCTCAAT 
GAGACCTTCT CTGAGCTGCG GCTGGACAGC GTGGTCCAGT GGATGGAGCA TTTCGAGACA 
TGGCCCTCCC ACCTCCCCAT TGTGGCTCAC GCAGAGCAGC AAACCGTGGC TGCTGTCCTC 
ATGGTGGCTC AGCTCACTCA GCGCTCAGTG CACATATGTC ACGTGGCACG GAAGGAGGAG 
ATCCTGCTAA TTAAAGCTGC AAAGGCACGG GGCTTGCCAG TGACCTGCGA GGTGGCTCCC 
CACCACCTGT TCCTAAGCCA TGATGACCTG GAGCGCCTGG GGCCTGGGAA GGGGGAGGTC 
CGGCCTGAGC TTGGCTCCCG CCAGGATGTG GAAGCCCTGT GGGAGAACAT GGCTGTCATC 
GACTGCTTTG CCTCAGACCA TGCTCCCCAT ACCTTGGAGG AGAAGTGTGG GTCCAGGCCC 
CCACCTGGGT TCCCAGGGTT AGAGACCATG CTGCCACTAC TCCTGACGGC TGTAAGCGAG 
GGCCGGCTCA GCCTGGACGA CCTGCTGCAG CGATTGCACC ACAATCCTCG GCGCATCTTT 
CACCTGCCCC CGCAGGAGGA CACCTATGTG GAGGTGGATC TGGAGCATGA GTGGACAATT 
CCCAGCCACA TGCCCTTCTC CAAGGCCCAC TGGACACCTT TTGAAGGGCA GAAAGTGAAG 
GGCACCGTCC GCCGTGTGGT CCTGCGAGGG GAGGTTGCCT ATATCGATGG GCAGGTTCTG 
GTACCCCCGG GCTATGGACA GGATGTACGG AAGTGGCCAC AGGGGGCTGT TCCTCAGCTC 
CCACCCTCAG CCCCTGCCAC TAGTGAGATG ACCACGACAC CTGAAAGACC CCGCCGTGGC 
ATCCCAGGGC TTCCTGATGG CCGCTTCCAT CTGCCGCCCC GAATCCATCG AGCCTCCGAC 
CCAGGTTTGC CAGCTGAGGA GCCAAAGGAG AAGTCCTCTC GGAAGGTAGC CGAGCCAGAG 
CTGATGGGAA CCCCTGATGG CACCTGCTAC CCTCCACCAC CAGTACCGAG ACAGGCATCT 
CCCCAGAACC TGGGGACCCC TGGCTTGCTG CACCCCCAGA CCTCACCCCT GCTGCACTCA 
TTAGTGGGCC AACATATCCT GTCCGTCCAG CAGTTCACCA AGGATCAGAT GTCTCACCTG 
TTCAATGTGG CACACACACT GCGTATGATG GTGCAGAAGG AGCGGAGCCT CGACATCCTG 
AAGGGGAAGG TCATGGCCTC CATGTTCTAT GAAGTGAGCA CACGGACCAG CAGCTCCTTT 
GCAGCAGCCA TGGCCCGGCT GGGAGGTGCT GTGCTCAGCT TCTCGGAAGC CACATCGTCC 
GTCCAGAAGG GCGAATCCCT GGCTGACTCC GTGCAGACCA TGAGCTGCTA TGCCGACGTC 
GTCGTGCTCC GGCACCCCCA GCCTGGAGCA GTGGAGCTGG CCGCCAAGCA CTGCCGGAGG 
CCAGTGATCA ATGCTGGGGA TGGGGTCGGA GAGCACCCCA CCCAGGCCCT GCTGGACATC 
TTCACCATCC GTGAGGAGCT GGGAACTGTC AATGGCATGA CGATCACGAT GGTGGGTGAC 
CTGAAGCACG GACGCACAGT ACATTCCCTG GCCTGCCTGC TCACCCAGTA TCGTGTCAGC 
CTGCGCTACG TGGCACCTCC CAGCCTGCGC ATGCCACCCA CTGTGCGGGC CTTCGTGGCC 
TCCCGCGGCA CCAAGCAGGA GGAATTCGAG AGCATTGAGG AGGCGCTGCC TGACACTGAT 
GTGCTCTACA TGACTCGAAT CCAGAAGGAA CGATTTGGCT CTACCCAGGA GTACGAAGCT 
TGCTTTGGTC AGTTCATCCT CACTCCCCAC ATCATGACCC GGGCCAAGAA GAAGATGGTG 
GTGATGCACC CGATGCCCCG TGTCAACGAG ATAAGCGTGG AAGTGGACTC GGATCCCCGC 
GCAGCCTACT TCCGCCAGGC TGAGAACGGC ATGTACATCC GCATGGCTCT GTTAGCCACC 
GTGCTGGGCC GTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( NM_001306079.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001293008.1 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 2, mRNA.

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CloneID	OHu35604
Clone ID Related Accession (Same CDS sequence)	NM_001306079.1
Accession Version	NM_001306079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	CAD protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC065510.1, AK226105.1, BG766243.1 and BQ447516.1. On Apr 14, 2015 this sequence version replaced XM_005264558.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226105.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGTATTACAT CATTGAAGTG 
AATGCCAGGC TCTCTCGCAG CTCTGCCCTG GCCAGTAAGG CCACAGGTTA TCCACTGGCT 
TATGTGGCAG CCAAGCTAGC ATTGGGCATC CCTTTGCCTG AGCTCAGGAA CTCTGTGACA 
GGGGGTACAG CAGCCTTTGA ACCCAGCGTG GATTATTGTG TGGTGAAGAT TCCTCGATGG 
GACCTTAGCA AGTTCCTGCG AGTCAGCACA AAGATTGGGA GCTGCATGAA GAGCGTTGGT 
GAAGTCATGG GCATTGGGCG TTCATTTGAG GAGGCCTTCC AGAAGGCCCT GCGCATGGTG 
GATGAGAACT GTGTGGGCTT TGATCACACA GTGAAACCAG TCAGCGATAT GGAGTTGGAG 
ACTCCAACAG ATAAGCGGAT TTTTGTGGTG GCAGCTGCTT TGTGGGCTGG TTATTCAGTG 
GACCGCCTGT ATGAGCTCAC ACGCATCGAC CGCTGGTTCC TGCACCGAAT GAAGCGTATC 
ATCGCACATG CCCAGCTGCT AGAACAACAC CGTGGACAGC CTTTGCCGCC AGACCTGCTG 
CAACAGGCCA AGTGTCTTGG CTTCTCAGAC AAACAGATTG CCCTTGCAGT TCTGAGCACA 
GAGCTGGCTG TTCGCAAGCT GCGTCAGGAA CTGGGGATCT GTCCAGCAGT GAAACAGATT 
GACACAGTTG CAGCTGAGTG GCCAGCCCAG ACAAATTACC TATACCTAAC GTATTGGGGC 
ACCACCCATG ACCTCACCTT TCGAACACCT CATGTCCTAG TCCTTGGCTC TGGCGTCTAC 
CGTATTGGCT CTAGCGTTGA ATTTGACTGG TGTGCTGTAG GCTGCATCCA GCAGCTCCGA 
AAGATGGGAT ATAAGACCAT CATGGTGAAC TATAACCCAG AGACAGTCAG CACCGACTAT 
GACATGTGTG ATCGACTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGACATCTAT 
GAGCTCGAGA ACCCTGAAGG TGTGATCCTA TCCATGGGTG GACAGCTGCC CAACAACATG 
GCCATGGCGT TGCATCGGCA GCAGTGCCGG GTGCTGGGCA CCTCCCCTGA AGCCATTGAC 
TCGGCTGAGA ACCGTTTCAA GTTTTCCCGG CTCCTTGACA CCATTGGTAT CAGCCAGCCT 
CAGTGGAGGG AGCTCAGTGA CCTCGAGTCT GCTCGCCAAT TCTGCCAGAC CGTGGGGTAC 
CCCTGTGTGG TGCGCCCCTC CTATGTGCTG AGCGGTGCTG CTATGAATGT GGCCTACACG 
GATGGAGACC TGGAGCGCTT CCTGAGCAGC GCAGCAGCCG TCTCCAAAGA GCATCCCGTG 
GTCATCTCCA AGTTCATCCA GGAGGCTAAG GAGATTGACG TGGATGCCGT GGCCTCTGAT 
GGTGTGGTGG CAGCCATCGC CATCTCTGAG CATGTGGAGA ATGCAGGTGT GCATTCAGGT 
GATGCGACGC TGGTGACCCC CCCACAAGAT ATCACTGCCA AAACCCTGGA GCGGATCAAA 
GCCATTGTGC ATGCTGTGGG CCAGGAGCTA CAGGTCACAG GACCCTTCAA TCTGCAGCTC 
ATTGCCAAGG ATGACCAGCT GAAAGTTATT GAATGCAACG TACGTGTCTC TCGCTCCTTC 
CCCTTCGTTT CCAAGACACT GGGTGTGGAC CTAGTAGCCT TGGCCACGCG GGTCATCATG 
GGGGAAGAAG TGGAACCTGT GGGGCTAATG ACTGGTTCTG GAGTCGTGGG AGTAAAGGTG 
CCTCAGTTCT CCTTCTCCCG CTTGGCGGGT GCTGACGTGG TGTTGGGTGT GGAAATGACC 
AGTACTGGGG AGGTGGCCGG CTTTGGGGAG AGCCGCTGTG AGGCATACCT CAAGGCCATG 
CTAAGCACTG GCTTTAAGAT CCCCAAGAAG AATATCCTGC TGACCATTGG CAGCTATAAG 
AACAAAAGCG AGCTGCTCCC AACTGTGCGG CTACTGGAGA GCCTGGGCTA CAGCCTCTAT 
GCCAGTCTCG GCACAGCTGA CTTCTACACT GAGCATGGCG TCAAGGTAAC AGCTGTGGAC 
TGGCACTTTG AGGAGGCTGT GGATGGTGAG TGCCCACCAC AGCGGAGCAT CCTGGAGCAG 
CTAGCTGAGA AAAACTTTGA GCTGGTGATT AACCTGTCAA TGCGTGGAGC TGGGGGCCGG 
CGTCTCTCTT CCTTTGTCAC CAAGGGCTAC CGCACCCGAC GCTTGGCCGC TGACTTCTCC 
GTGCCCCTAA TCATCGATAT CAAGTGCACC AAACTCTTTG TGGAGGCCCT AGGCCAGATC 
GGGCCAGCCC CTCCTTTGAA GGTGCATGTT GACTGTATGA CCTCCCAAAA GCTTGTGCGA 
CTGCCGGGAT TGATTGATGT CCATGTGCAC CTGCGGGAAC CAGGTGGGAC ACATAAGGAG 
GACTTTGCTT CAGGCACAGC CGCTGCCCTG GCTGGGGGTA TCACCATGGT GTGTGCCATG 
CCTAATACCC GGCCCCCCAT CATTGACGCC CCTGCTCTGG CCCTGGCCCA GAAGCTGGCA 
GAGGCTGGCG CCCGGTGCGA CTTTGCGCTA TTCCTTGGGG CCTCGTCTGA AAATGCAGGA 
ACCTTGGGCA CCGTGGCCGG GTCTGCAGCC GGGCTGAAGC TTTACCTCAA TGAGACCTTC 
TCTGAGCTGC GGCTGGACAG CGTGGTCCAG TGGATGGAGC ATTTCGAGAC ATGGCCCTCC 
CACCTCCCCA TTGTGGCTCA CGCAGAGCAG CAAACCGTGG CTGCTGTCCT CATGGTGGCT 
CAGCTCACTC AGCGCTCAGT GCACATATGT CACGTGGCAC GGAAGGAGGA GATCCTGCTA 
ATTAAAGCTG CAAAGGCACG GGGCTTGCCA GTGACCTGCG AGGTGGCTCC CCACCACCTG 
TTCCTAAGCC ATGATGACCT GGAGCGCCTG GGGCCTGGGA AGGGGGAGGT CCGGCCTGAG 
CTTGGCTCCC GCCAGGATGT GGAAGCCCTG TGGGAGAACA TGGCTGTCAT CGACTGCTTT 
GCCTCAGACC ATGCTCCCCA TACCTTGGAG GAGAAGTGTG GGTCCAGGCC CCCACCTGGG 
TTCCCAGGGT TAGAGACCAT GCTGCCACTA CTCCTGACGG CTGTAAGCGA GGGCCGGCTC 
AGCCTGGACG ACCTGCTGCA GCGATTGCAC CACAATCCTC GGCGCATCTT TCACCTGCCC 
CCGCAGGAGG ACACCTATGT GGAGGTGGAT CTGGAGCATG AGTGGACAAT TCCCAGCCAC 
ATGCCCTTCT CCAAGGCCCA CTGGACACCT TTTGAAGGGC AGAAAGTGAA GGGCACCGTC 
CGCCGTGTGG TCCTGCGAGG GGAGGTTGCC TATATCGATG GGCAGGTTCT GGTACCCCCG 
GGCTATGGAC AGGATGTACG GAAGTGGCCA CAGGGGGCTG TTCCTCAGCT CCCACCCTCA 
GCCCCTGCCA CTAGTGAGAT GACCACGACA CCTGAAAGAC CCCGCCGTGG CATCCCAGGG 
CTTCCTGATG GCCGCTTCCA TCTGCCGCCC CGAATCCATC GAGCCTCCGA CCCAGGTTTG 
CCAGCTGAGG AGCCAAAGGA GAAGTCCTCT CGGAAGGTAG CCGAGCCAGA GCTGATGGGA 
ACCCCTGATG GCACCTGCTA CCCTCCACCA CCAGTACCGA GACAGGCATC TCCCCAGAAC 
CTGGGGACCC CTGGCTTGCT GCACCCCCAG ACCTCACCCC TGCTGCACTC ATTAGTGGGC 
CAACATATCC TGTCCGTCCA GCAGTTCACC AAGGATCAGA TGTCTCACCT GTTCAATGTG 
GCACACACAC TGCGTATGAT GGTGCAGAAG GAGCGGAGCC TCGACATCCT GAAGGGGAAG 
GTCATGGCCT CCATGTTCTA TGAAGTGAGC ACACGGACCA GCAGCTCCTT TGCAGCAGCC 
ATGGCCCGGC TGGGAGGTGC TGTGCTCAGC TTCTCGGAAG CCACATCGTC CGTCCAGAAG 
GGCGAATCCC TGGCTGACTC CGTGCAGACC ATGAGCTGCT ATGCCGACGT CGTCGTGCTC 
CGGCACCCCC AGCCTGGAGC AGTGGAGCTG GCCGCCAAGC ACTGCCGGAG GCCAGTGATC 
AATGCTGGGG ATGGGGTCGG AGAGCACCCC ACCCAGGCCC TGCTGGACAT CTTCACCATC 
CGTGAGGAGC TGGGAACTGT CAATGGCATG ACGATCACGA TGGTGGGTGA CCTGAAGCAC 
GGACGCACAG TACATTCCCT GGCCTGCCTG CTCACCCAGT ATCGTGTCAG CCTGCGCTAC 
GTGGCACCTC CCAGCCTGCG CATGCCACCC ACTGTGCGGG CCTTCGTGGC CTCCCGCGGC 
ACCAAGCAGG AGGAATTCGA GAGCATTGAG GAGGCGCTGC CTGACACTGA TGTGCTCTAC 
ATGACTCGAA TCCAGAAGGA ACGATTTGGC TCTACCCAGG AGTACGAAGC TTGCTTTGGT 
CAGTTCATCC TCACTCCCCA CATCATGACC CGGGCCAAGA AGAAGATGGT GGTGATGCAC 
CCGATGCCCC GTGTCAACGA GATAAGCGTG GAAGTGGACT CGGATCCCCG CGCAGCCTAC 
TTCCGCCAGG CTGAGAACGG CATGTACATC CGCATGGCTC TGTTAGCCAC CGTGCTGGGC 
CGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001293008.1
CDS	163..6651
Translation	MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	NM_001306079.1
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306079.1

ORF Insert Sequence:

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGTATTACAT CATTGAAGTG 
AATGCCAGGC TCTCTCGCAG CTCTGCCCTG GCCAGTAAGG CCACAGGTTA TCCACTGGCT 
TATGTGGCAG CCAAGCTAGC ATTGGGCATC CCTTTGCCTG AGCTCAGGAA CTCTGTGACA 
GGGGGTACAG CAGCCTTTGA ACCCAGCGTG GATTATTGTG TGGTGAAGAT TCCTCGATGG 
GACCTTAGCA AGTTCCTGCG AGTCAGCACA AAGATTGGGA GCTGCATGAA GAGCGTTGGT 
GAAGTCATGG GCATTGGGCG TTCATTTGAG GAGGCCTTCC AGAAGGCCCT GCGCATGGTG 
GATGAGAACT GTGTGGGCTT TGATCACACA GTGAAACCAG TCAGCGATAT GGAGTTGGAG 
ACTCCAACAG ATAAGCGGAT TTTTGTGGTG GCAGCTGCTT TGTGGGCTGG TTATTCAGTG 
GACCGCCTGT ATGAGCTCAC ACGCATCGAC CGCTGGTTCC TGCACCGAAT GAAGCGTATC 
ATCGCACATG CCCAGCTGCT AGAACAACAC CGTGGACAGC CTTTGCCGCC AGACCTGCTG 
CAACAGGCCA AGTGTCTTGG CTTCTCAGAC AAACAGATTG CCCTTGCAGT TCTGAGCACA 
GAGCTGGCTG TTCGCAAGCT GCGTCAGGAA CTGGGGATCT GTCCAGCAGT GAAACAGATT 
GACACAGTTG CAGCTGAGTG GCCAGCCCAG ACAAATTACC TATACCTAAC GTATTGGGGC 
ACCACCCATG ACCTCACCTT TCGAACACCT CATGTCCTAG TCCTTGGCTC TGGCGTCTAC 
CGTATTGGCT CTAGCGTTGA ATTTGACTGG TGTGCTGTAG GCTGCATCCA GCAGCTCCGA 
AAGATGGGAT ATAAGACCAT CATGGTGAAC TATAACCCAG AGACAGTCAG CACCGACTAT 
GACATGTGTG ATCGACTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGACATCTAT 
GAGCTCGAGA ACCCTGAAGG TGTGATCCTA TCCATGGGTG GACAGCTGCC CAACAACATG 
GCCATGGCGT TGCATCGGCA GCAGTGCCGG GTGCTGGGCA CCTCCCCTGA AGCCATTGAC 
TCGGCTGAGA ACCGTTTCAA GTTTTCCCGG CTCCTTGACA CCATTGGTAT CAGCCAGCCT 
CAGTGGAGGG AGCTCAGTGA CCTCGAGTCT GCTCGCCAAT TCTGCCAGAC CGTGGGGTAC 
CCCTGTGTGG TGCGCCCCTC CTATGTGCTG AGCGGTGCTG CTATGAATGT GGCCTACACG 
GATGGAGACC TGGAGCGCTT CCTGAGCAGC GCAGCAGCCG TCTCCAAAGA GCATCCCGTG 
GTCATCTCCA AGTTCATCCA GGAGGCTAAG GAGATTGACG TGGATGCCGT GGCCTCTGAT 
GGTGTGGTGG CAGCCATCGC CATCTCTGAG CATGTGGAGA ATGCAGGTGT GCATTCAGGT 
GATGCGACGC TGGTGACCCC CCCACAAGAT ATCACTGCCA AAACCCTGGA GCGGATCAAA 
GCCATTGTGC ATGCTGTGGG CCAGGAGCTA CAGGTCACAG GACCCTTCAA TCTGCAGCTC 
ATTGCCAAGG ATGACCAGCT GAAAGTTATT GAATGCAACG TACGTGTCTC TCGCTCCTTC 
CCCTTCGTTT CCAAGACACT GGGTGTGGAC CTAGTAGCCT TGGCCACGCG GGTCATCATG 
GGGGAAGAAG TGGAACCTGT GGGGCTAATG ACTGGTTCTG GAGTCGTGGG AGTAAAGGTG 
CCTCAGTTCT CCTTCTCCCG CTTGGCGGGT GCTGACGTGG TGTTGGGTGT GGAAATGACC 
AGTACTGGGG AGGTGGCCGG CTTTGGGGAG AGCCGCTGTG AGGCATACCT CAAGGCCATG 
CTAAGCACTG GCTTTAAGAT CCCCAAGAAG AATATCCTGC TGACCATTGG CAGCTATAAG 
AACAAAAGCG AGCTGCTCCC AACTGTGCGG CTACTGGAGA GCCTGGGCTA CAGCCTCTAT 
GCCAGTCTCG GCACAGCTGA CTTCTACACT GAGCATGGCG TCAAGGTAAC AGCTGTGGAC 
TGGCACTTTG AGGAGGCTGT GGATGGTGAG TGCCCACCAC AGCGGAGCAT CCTGGAGCAG 
CTAGCTGAGA AAAACTTTGA GCTGGTGATT AACCTGTCAA TGCGTGGAGC TGGGGGCCGG 
CGTCTCTCTT CCTTTGTCAC CAAGGGCTAC CGCACCCGAC GCTTGGCCGC TGACTTCTCC 
GTGCCCCTAA TCATCGATAT CAAGTGCACC AAACTCTTTG TGGAGGCCCT AGGCCAGATC 
GGGCCAGCCC CTCCTTTGAA GGTGCATGTT GACTGTATGA CCTCCCAAAA GCTTGTGCGA 
CTGCCGGGAT TGATTGATGT CCATGTGCAC CTGCGGGAAC CAGGTGGGAC ACATAAGGAG 
GACTTTGCTT CAGGCACAGC CGCTGCCCTG GCTGGGGGTA TCACCATGGT GTGTGCCATG 
CCTAATACCC GGCCCCCCAT CATTGACGCC CCTGCTCTGG CCCTGGCCCA GAAGCTGGCA 
GAGGCTGGCG CCCGGTGCGA CTTTGCGCTA TTCCTTGGGG CCTCGTCTGA AAATGCAGGA 
ACCTTGGGCA CCGTGGCCGG GTCTGCAGCC GGGCTGAAGC TTTACCTCAA TGAGACCTTC 
TCTGAGCTGC GGCTGGACAG CGTGGTCCAG TGGATGGAGC ATTTCGAGAC ATGGCCCTCC 
CACCTCCCCA TTGTGGCTCA CGCAGAGCAG CAAACCGTGG CTGCTGTCCT CATGGTGGCT 
CAGCTCACTC AGCGCTCAGT GCACATATGT CACGTGGCAC GGAAGGAGGA GATCCTGCTA 
ATTAAAGCTG CAAAGGCACG GGGCTTGCCA GTGACCTGCG AGGTGGCTCC CCACCACCTG 
TTCCTAAGCC ATGATGACCT GGAGCGCCTG GGGCCTGGGA AGGGGGAGGT CCGGCCTGAG 
CTTGGCTCCC GCCAGGATGT GGAAGCCCTG TGGGAGAACA TGGCTGTCAT CGACTGCTTT 
GCCTCAGACC ATGCTCCCCA TACCTTGGAG GAGAAGTGTG GGTCCAGGCC CCCACCTGGG 
TTCCCAGGGT TAGAGACCAT GCTGCCACTA CTCCTGACGG CTGTAAGCGA GGGCCGGCTC 
AGCCTGGACG ACCTGCTGCA GCGATTGCAC CACAATCCTC GGCGCATCTT TCACCTGCCC 
CCGCAGGAGG ACACCTATGT GGAGGTGGAT CTGGAGCATG AGTGGACAAT TCCCAGCCAC 
ATGCCCTTCT CCAAGGCCCA CTGGACACCT TTTGAAGGGC AGAAAGTGAA GGGCACCGTC 
CGCCGTGTGG TCCTGCGAGG GGAGGTTGCC TATATCGATG GGCAGGTTCT GGTACCCCCG 
GGCTATGGAC AGGATGTACG GAAGTGGCCA CAGGGGGCTG TTCCTCAGCT CCCACCCTCA 
GCCCCTGCCA CTAGTGAGAT GACCACGACA CCTGAAAGAC CCCGCCGTGG CATCCCAGGG 
CTTCCTGATG GCCGCTTCCA TCTGCCGCCC CGAATCCATC GAGCCTCCGA CCCAGGTTTG 
CCAGCTGAGG AGCCAAAGGA GAAGTCCTCT CGGAAGGTAG CCGAGCCAGA GCTGATGGGA 
ACCCCTGATG GCACCTGCTA CCCTCCACCA CCAGTACCGA GACAGGCATC TCCCCAGAAC 
CTGGGGACCC CTGGCTTGCT GCACCCCCAG ACCTCACCCC TGCTGCACTC ATTAGTGGGC 
CAACATATCC TGTCCGTCCA GCAGTTCACC AAGGATCAGA TGTCTCACCT GTTCAATGTG 
GCACACACAC TGCGTATGAT GGTGCAGAAG GAGCGGAGCC TCGACATCCT GAAGGGGAAG 
GTCATGGCCT CCATGTTCTA TGAAGTGAGC ACACGGACCA GCAGCTCCTT TGCAGCAGCC 
ATGGCCCGGC TGGGAGGTGC TGTGCTCAGC TTCTCGGAAG CCACATCGTC CGTCCAGAAG 
GGCGAATCCC TGGCTGACTC CGTGCAGACC ATGAGCTGCT ATGCCGACGT CGTCGTGCTC 
CGGCACCCCC AGCCTGGAGC AGTGGAGCTG GCCGCCAAGC ACTGCCGGAG GCCAGTGATC 
AATGCTGGGG ATGGGGTCGG AGAGCACCCC ACCCAGGCCC TGCTGGACAT CTTCACCATC 
CGTGAGGAGC TGGGAACTGT CAATGGCATG ACGATCACGA TGGTGGGTGA CCTGAAGCAC 
GGACGCACAG TACATTCCCT GGCCTGCCTG CTCACCCAGT ATCGTGTCAG CCTGCGCTAC 
GTGGCACCTC CCAGCCTGCG CATGCCACCC ACTGTGCGGG CCTTCGTGGC CTCCCGCGGC 
ACCAAGCAGG AGGAATTCGA GAGCATTGAG GAGGCGCTGC CTGACACTGA TGTGCTCTAC 
ATGACTCGAA TCCAGAAGGA ACGATTTGGC TCTACCCAGG AGTACGAAGC TTGCTTTGGT 
CAGTTCATCC TCACTCCCCA CATCATGACC CGGGCCAAGA AGAAGATGGT GGTGATGCAC 
CCGATGCCCC GTGTCAACGA GATAAGCGTG GAAGTGGACT CGGATCCCCG CGCAGCCTAC 
TTCCGCCAGG CTGAGAACGG CATGTACATC CGCATGGCTC TGTTAGCCAC CGTGCTGGGC 
CGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_005264555.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264612.1 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.

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CloneID	OHu35600
Clone ID Related Accession (Same CDS sequence)	XM_005264555.3
Accession Version	XM_005264555.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264555.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGGTGTGTAA CATGGAGAAC 
TTGGACCCAC TGGGCATCCA CACTGGTGAG TCCATAGTGG TGGCCCCTAG CCAGACACTG 
AATGACAGGG AGTATCAGCT CCTGAGGCAG ACAGCTATCA AGGTGACCCA GCACCTGGGA 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCTG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCTCGCAGC TCTGCCCTGG CCAGTAAGGC CACAGGTTAT 
CCACTGGCTT ATGTGGCAGC CAAGCTAGCA TTGGGCATCC CTTTGCCTGA GCTCAGGAAC 
TCTGTGACAG GGGGTACAGC AGCCTTTGAA CCCAGCGTGG ATTATTGTGT GGTGAAGATT 
CCTCGATGGG ACCTTAGCAA GTTCCTGCGA GTCAGCACAA AGATTGGGAG CTGCATGAAG 
AGCGTTGGTG AAGTCATGGG CATTGGGCGT TCATTTGAGG AGGCCTTCCA GAAGGCCCTG 
CGCATGGTGG ATGAGAACTG TGTGGGCTTT GATCACACAG TGAAACCAGT CAGCGATATG 
GAGTTGGAGA CTCCAACAGA TAAGCGGATT TTTGTGGTGG CAGCTGCTTT GTGGGCTGGT 
TATTCAGTGG ACCGCCTGTA TGAGCTCACA CGCATCGACC GCTGGTTCCT GCACCGAATG 
AAGCGTATCA TCGCACATGC CCAGCTGCTA GAACAACACC GTGGACAGCC TTTGCCGCCA 
GACCTGCTGC AACAGGCCAA GTGTCTTGGC TTCTCAGACA AACAGATTGC CCTTGCAGTT 
CTGAGCACAG AGCTGGCTGT TCGCAAGCTG CGTCAGGAAC TGGGGATCTG TCCAGCAGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT ATACCTAACG 
TATTGGGGCA CCACCCATGA CCTCACCTTT CGAACACCTC ATGTCCTAGT CCTTGGCTCT 
GGCGTCTACC GTATTGGCTC TAGCGTTGAA TTTGACTGGT GTGCTGTAGG CTGCATCCAG 
CAGCTCCGAA AGATGGGATA TAAGACCATC ATGGTGAACT ATAACCCAGA GACAGTCAGC 
ACCGACTATG ACATGTGTGA TCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGCTCGAGAA CCCTGAAGGT GTGATCCTAT CCATGGGTGG ACAGCTGCCC 
AACAACATGG CCATGGCGTT GCATCGGCAG CAGTGCCGGG TGCTGGGCAC CTCCCCTGAA 
GCCATTGACT CGGCTGAGAA CCGTTTCAAG TTTTCCCGGC TCCTTGACAC CATTGGTATC 
AGCCAGCCTC AGTGGAGGGA GCTCAGTGAC CTCGAGTCTG CTCGCCAATT CTGCCAGACC 
GTGGGGTACC CCTGTGTGGT GCGCCCCTCC TATGTGCTGA GCGGTGCTGC TATGAATGTG 
GCCTACACGG ATGGAGACCT GGAGCGCTTC CTGAGCAGCG CAGCAGCCGT CTCCAAAGAG 
CATCCCGTGG TCATCTCCAA GTTCATCCAG GAGGCTAAGG AGATTGACGT GGATGCCGTG 
GCCTCTGATG GTGTGGTGGC AGCCATCGCC ATCTCTGAGC ATGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCGACGCT GGTGACCCCC CCACAAGATA TCACTGCCAA AACCCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAAGTTATTG AATGCAACGT ACGTGTCTCT 
CGCTCCTTCC CCTTCGTTTC CAAGACACTG GGTGTGGACC TAGTAGCCTT GGCCACGCGG 
GTCATCATGG GGGAAGAAGT GGAACCTGTG GGGCTAATGA CTGGTTCTGG AGTCGTGGGA 
GTAAAGGTGC CTCAGTTCTC CTTCTCCCGC TTGGCGGGTG CTGACGTGGT GTTGGGTGTG 
GAAATGACCA GTACTGGGGA GGTGGCCGGC TTTGGGGAGA GCCGCTGTGA GGCATACCTC 
AAGGCCATGC TAAGCACTGG CTTTAAGATC CCCAAGAAGA ATATCCTGCT GACCATTGGC 
AGCTATAAGA ACAAAAGCGA GCTGCTCCCA ACTGTGCGGC TACTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGTCTCGG CACAGCTGAC TTCTACACTG AGCATGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA GGAGGCTGTG GATGGTGAGT GCCCACCACA GCGGAGCATC 
CTGGAGCAGC TAGCTGAGAA AAACTTTGAG CTGGTGATTA ACCTGTCAAT GCGTGGAGCT 
GGGGGCCGGC GTCTCTCTTC CTTTGTCACC AAGGGCTACC GCACCCGACG CTTGGCCGCT 
GACTTCTCCG TGCCCCTAAT CATCGATATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAG GTGCATGTTG ACTGTATGAC CTCCCAAAAG 
CTTGTGCGAC TGCCGGGATT GATTGATGTC CATGTGCACC TGCGGGAACC AGGTGGGACA 
CATAAGGAGG ACTTTGCTTC AGGCACAGCC GCTGCCCTGG CTGGGGGTAT CACCATGGTG 
TGTGCCATGC CTAATACCCG GCCCCCCATC ATTGACGCCC CTGCTCTGGC CCTGGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGGTGCGAC TTTGCGCTAT TCCTTGGGGC CTCGTCTGAA 
AATGCAGGAA CCTTGGGCAC CGTGGCCGGG TCTGCAGCCG GGCTGAAGCT TTACCTCAAT 
GAGACCTTCT CTGAGCTGCG GCTGGACAGC GTGGTCCAGT GGATGGAGCA TTTCGAGACA 
TGGCCCTCCC ACCTCCCCAT TGTGGCTCAC GCAGAGCAGC AAACCGTGGC TGCTGTCCTC 
ATGGTGGCTC AGCTCACTCA GCGCTCAGTG CACATATGTC ACGTGGCACG GAAGGAGGAG 
ATCCTGCTAA TTAAAGCTGC AAAGGCACGG GGCTTGCCAG TGACCTGCGA GGTGGCTCCC 
CACCACCTGT TCCTAAGCCA TGATGACCTG GAGCGCCTGG GGCCTGGGAA GGGGGAGGTC 
CGGCCTGAGC TTGGCTCCCG CCAGGATGTG GAAGCCCTGT GGGAGAACAT GGCTGTCATC 
GACTGCTTTG CCTCAGACCA TGCTCCCCAT ACCTTGGAGG AGAAGTGTGG GTCCAGGCCC 
CCACCTGGGT TCCCAGGGTT AGAGACCATG CTGCCACTAC TCCTGACGGC TGTAAGCGAG 
GGCCGGCTCA GCCTGGACGA CCTGCTGCAG CGATTGCACC ACAATCCTCG GCGCATCTTT 
CACCTGCCCC CGCAGGAGGA CACCTATGTG GAGGTGGATC TGGAGCATGA GTGGACAATT 
CCCAGCCACA TGCCCTTCTC CAAGGCCCAC TGGACACCTT TTGAAGGGCA GAAAGTGAAG 
GGCACCGTCC GCCGTGTGGT CCTGCGAGGG GAGGTTGCCT ATATCGATGG GCAGGTTCTG 
GTACCCCCGG GCTATGGACA GGATGTACGG AAGTGGCCAC AGGGGGCTGT TCCTCAGCTC 
CCACCCTCAG CCCCTGCCAC TAGTGAGATG ACCACGACAC CTGAAAGACC CCGCCGTGGC 
ATCCCAGGGC TTCCTGATGG CCGCTTCCAT CTGCCGCCCC GAATCCATCG AGCCTCCGAC 
CCAGGTTTGC CAGCTGTGTT CCTCCGCCCA GGAGCTGGGA TCCCACGGGG CAGCAGAGCG 
TGGGCTGAGG AGCCAAAGGA GAAGTCCTCT CGGAAGGTAG CCGAGCCAGA GCTGATGGGA 
ACCCCTGATG GCACCTGCTA CCCTCCACCA CCAGTACCGA GACAGGCATC TCCCCAGAAC 
CTGGGGACCC CTGGCTTGCT GCACCCCCAG ACCTCACCCC TGCTGCACTC ATTAGTGGGC 
CAACATATCC TGTCCGTCCA GCAGTTCACC AAGGATCAGA TGTCTCACCT GTTCAATGTG 
GCACACACAC TGCGTATGAT GGTGCAGAAG GAGCGGAGCC TCGACATCCT GAAGGGGAAG 
GTCATGGCCT CCATGTTCTA TGAAGTGAGC ACACGGACCA GCAGCTCCTT TGCAGCAGCC 
ATGGCCCGGC TGGGAGGTGC TGTGCTCAGC TTCTCGGAAG CCACATCGTC CGTCCAGAAG 
GGCGAATCCC TGGCTGACTC CGTGCAGACC ATGAGCTGCT ATGCCGACGT CGTCGTGCTC 
CGGCACCCCC AGCCTGGAGC AGTGGAGCTG GCCGCCAAGC ACTGCCGGAG GCCAGTGATC 
AATGCTGGGG ATGGGGTCGG AGAGCACCCC ACCCAGGCCC TGCTGGACAT CTTCACCATC 
CGTGAGGAGC TGGGAACTGT CAATGGCATG ACGATCACGA TGGTGGGTGA CCTGAAGCAC 
GGACGCACAG TACATTCCCT GGCCTGCCTG CTCACCCAGT ATCGTGTCAG CCTGCGCTAC 
GTGGCACCTC CCAGCCTGCG CATGCCACCC ACTGTGCGGG CCTTCGTGGC CTCCCGCGGC 
ACCAAGCAGG AGGAATTCGA GAGCATTGAG GAGGCGCTGC CTGACACTGA TGTGCTCTAC 
ATGACTCGAA TCCAGAAGGA ACGATTTGGC TCTACCCAGG AGTACGAAGC TTGCTTTGGT 
CAGTTCATCC TCACTCCCCA CATCATGACC CGGGCCAAGA AGAAGATGGT GGTGATGCAC 
CCGATGCCCC GTGTCAACGA GATAAGCGTG GAAGTGGACT CGGATCCCCG CGCAGCCTAC 
TTCCGCCAGG CTGAGAACGG CATGTACATC CGCATGGCTC TGTTAGCCAC CGTGCTGGGC 
CGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264612.1
CDS	296..7024
Translation	MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTVCNMENLDPLGIHTGESIVVAPSQTLNDREYQLLRQTAIKVTQHLGIVGECNVQYALNPESEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAVFLRPGAGIPRGSRAWAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	XM_005264555.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264555.3

ORF Insert Sequence:

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGGTGTGTAA CATGGAGAAC 
TTGGACCCAC TGGGCATCCA CACTGGTGAG TCCATAGTGG TGGCCCCTAG CCAGACACTG 
AATGACAGGG AGTATCAGCT CCTGAGGCAG ACAGCTATCA AGGTGACCCA GCACCTGGGA 
ATTGTTGGGG AGTGCAATGT GCAGTATGCC TTGAACCCTG AGTCTGAGCA GTATTACATC 
ATTGAAGTGA ATGCCAGGCT CTCTCGCAGC TCTGCCCTGG CCAGTAAGGC CACAGGTTAT 
CCACTGGCTT ATGTGGCAGC CAAGCTAGCA TTGGGCATCC CTTTGCCTGA GCTCAGGAAC 
TCTGTGACAG GGGGTACAGC AGCCTTTGAA CCCAGCGTGG ATTATTGTGT GGTGAAGATT 
CCTCGATGGG ACCTTAGCAA GTTCCTGCGA GTCAGCACAA AGATTGGGAG CTGCATGAAG 
AGCGTTGGTG AAGTCATGGG CATTGGGCGT TCATTTGAGG AGGCCTTCCA GAAGGCCCTG 
CGCATGGTGG ATGAGAACTG TGTGGGCTTT GATCACACAG TGAAACCAGT CAGCGATATG 
GAGTTGGAGA CTCCAACAGA TAAGCGGATT TTTGTGGTGG CAGCTGCTTT GTGGGCTGGT 
TATTCAGTGG ACCGCCTGTA TGAGCTCACA CGCATCGACC GCTGGTTCCT GCACCGAATG 
AAGCGTATCA TCGCACATGC CCAGCTGCTA GAACAACACC GTGGACAGCC TTTGCCGCCA 
GACCTGCTGC AACAGGCCAA GTGTCTTGGC TTCTCAGACA AACAGATTGC CCTTGCAGTT 
CTGAGCACAG AGCTGGCTGT TCGCAAGCTG CGTCAGGAAC TGGGGATCTG TCCAGCAGTG 
AAACAGATTG ACACAGTTGC AGCTGAGTGG CCAGCCCAGA CAAATTACCT ATACCTAACG 
TATTGGGGCA CCACCCATGA CCTCACCTTT CGAACACCTC ATGTCCTAGT CCTTGGCTCT 
GGCGTCTACC GTATTGGCTC TAGCGTTGAA TTTGACTGGT GTGCTGTAGG CTGCATCCAG 
CAGCTCCGAA AGATGGGATA TAAGACCATC ATGGTGAACT ATAACCCAGA GACAGTCAGC 
ACCGACTATG ACATGTGTGA TCGACTCTAC TTTGATGAGA TCTCTTTTGA GGTGGTGATG 
GACATCTATG AGCTCGAGAA CCCTGAAGGT GTGATCCTAT CCATGGGTGG ACAGCTGCCC 
AACAACATGG CCATGGCGTT GCATCGGCAG CAGTGCCGGG TGCTGGGCAC CTCCCCTGAA 
GCCATTGACT CGGCTGAGAA CCGTTTCAAG TTTTCCCGGC TCCTTGACAC CATTGGTATC 
AGCCAGCCTC AGTGGAGGGA GCTCAGTGAC CTCGAGTCTG CTCGCCAATT CTGCCAGACC 
GTGGGGTACC CCTGTGTGGT GCGCCCCTCC TATGTGCTGA GCGGTGCTGC TATGAATGTG 
GCCTACACGG ATGGAGACCT GGAGCGCTTC CTGAGCAGCG CAGCAGCCGT CTCCAAAGAG 
CATCCCGTGG TCATCTCCAA GTTCATCCAG GAGGCTAAGG AGATTGACGT GGATGCCGTG 
GCCTCTGATG GTGTGGTGGC AGCCATCGCC ATCTCTGAGC ATGTGGAGAA TGCAGGTGTG 
CATTCAGGTG ATGCGACGCT GGTGACCCCC CCACAAGATA TCACTGCCAA AACCCTGGAG 
CGGATCAAAG CCATTGTGCA TGCTGTGGGC CAGGAGCTAC AGGTCACAGG ACCCTTCAAT 
CTGCAGCTCA TTGCCAAGGA TGACCAGCTG AAAGTTATTG AATGCAACGT ACGTGTCTCT 
CGCTCCTTCC CCTTCGTTTC CAAGACACTG GGTGTGGACC TAGTAGCCTT GGCCACGCGG 
GTCATCATGG GGGAAGAAGT GGAACCTGTG GGGCTAATGA CTGGTTCTGG AGTCGTGGGA 
GTAAAGGTGC CTCAGTTCTC CTTCTCCCGC TTGGCGGGTG CTGACGTGGT GTTGGGTGTG 
GAAATGACCA GTACTGGGGA GGTGGCCGGC TTTGGGGAGA GCCGCTGTGA GGCATACCTC 
AAGGCCATGC TAAGCACTGG CTTTAAGATC CCCAAGAAGA ATATCCTGCT GACCATTGGC 
AGCTATAAGA ACAAAAGCGA GCTGCTCCCA ACTGTGCGGC TACTGGAGAG CCTGGGCTAC 
AGCCTCTATG CCAGTCTCGG CACAGCTGAC TTCTACACTG AGCATGGCGT CAAGGTAACA 
GCTGTGGACT GGCACTTTGA GGAGGCTGTG GATGGTGAGT GCCCACCACA GCGGAGCATC 
CTGGAGCAGC TAGCTGAGAA AAACTTTGAG CTGGTGATTA ACCTGTCAAT GCGTGGAGCT 
GGGGGCCGGC GTCTCTCTTC CTTTGTCACC AAGGGCTACC GCACCCGACG CTTGGCCGCT 
GACTTCTCCG TGCCCCTAAT CATCGATATC AAGTGCACCA AACTCTTTGT GGAGGCCCTA 
GGCCAGATCG GGCCAGCCCC TCCTTTGAAG GTGCATGTTG ACTGTATGAC CTCCCAAAAG 
CTTGTGCGAC TGCCGGGATT GATTGATGTC CATGTGCACC TGCGGGAACC AGGTGGGACA 
CATAAGGAGG ACTTTGCTTC AGGCACAGCC GCTGCCCTGG CTGGGGGTAT CACCATGGTG 
TGTGCCATGC CTAATACCCG GCCCCCCATC ATTGACGCCC CTGCTCTGGC CCTGGCCCAG 
AAGCTGGCAG AGGCTGGCGC CCGGTGCGAC TTTGCGCTAT TCCTTGGGGC CTCGTCTGAA 
AATGCAGGAA CCTTGGGCAC CGTGGCCGGG TCTGCAGCCG GGCTGAAGCT TTACCTCAAT 
GAGACCTTCT CTGAGCTGCG GCTGGACAGC GTGGTCCAGT GGATGGAGCA TTTCGAGACA 
TGGCCCTCCC ACCTCCCCAT TGTGGCTCAC GCAGAGCAGC AAACCGTGGC TGCTGTCCTC 
ATGGTGGCTC AGCTCACTCA GCGCTCAGTG CACATATGTC ACGTGGCACG GAAGGAGGAG 
ATCCTGCTAA TTAAAGCTGC AAAGGCACGG GGCTTGCCAG TGACCTGCGA GGTGGCTCCC 
CACCACCTGT TCCTAAGCCA TGATGACCTG GAGCGCCTGG GGCCTGGGAA GGGGGAGGTC 
CGGCCTGAGC TTGGCTCCCG CCAGGATGTG GAAGCCCTGT GGGAGAACAT GGCTGTCATC 
GACTGCTTTG CCTCAGACCA TGCTCCCCAT ACCTTGGAGG AGAAGTGTGG GTCCAGGCCC 
CCACCTGGGT TCCCAGGGTT AGAGACCATG CTGCCACTAC TCCTGACGGC TGTAAGCGAG 
GGCCGGCTCA GCCTGGACGA CCTGCTGCAG CGATTGCACC ACAATCCTCG GCGCATCTTT 
CACCTGCCCC CGCAGGAGGA CACCTATGTG GAGGTGGATC TGGAGCATGA GTGGACAATT 
CCCAGCCACA TGCCCTTCTC CAAGGCCCAC TGGACACCTT TTGAAGGGCA GAAAGTGAAG 
GGCACCGTCC GCCGTGTGGT CCTGCGAGGG GAGGTTGCCT ATATCGATGG GCAGGTTCTG 
GTACCCCCGG GCTATGGACA GGATGTACGG AAGTGGCCAC AGGGGGCTGT TCCTCAGCTC 
CCACCCTCAG CCCCTGCCAC TAGTGAGATG ACCACGACAC CTGAAAGACC CCGCCGTGGC 
ATCCCAGGGC TTCCTGATGG CCGCTTCCAT CTGCCGCCCC GAATCCATCG AGCCTCCGAC 
CCAGGTTTGC CAGCTGTGTT CCTCCGCCCA GGAGCTGGGA TCCCACGGGG CAGCAGAGCG 
TGGGCTGAGG AGCCAAAGGA GAAGTCCTCT CGGAAGGTAG CCGAGCCAGA GCTGATGGGA 
ACCCCTGATG GCACCTGCTA CCCTCCACCA CCAGTACCGA GACAGGCATC TCCCCAGAAC 
CTGGGGACCC CTGGCTTGCT GCACCCCCAG ACCTCACCCC TGCTGCACTC ATTAGTGGGC 
CAACATATCC TGTCCGTCCA GCAGTTCACC AAGGATCAGA TGTCTCACCT GTTCAATGTG 
GCACACACAC TGCGTATGAT GGTGCAGAAG GAGCGGAGCC TCGACATCCT GAAGGGGAAG 
GTCATGGCCT CCATGTTCTA TGAAGTGAGC ACACGGACCA GCAGCTCCTT TGCAGCAGCC 
ATGGCCCGGC TGGGAGGTGC TGTGCTCAGC TTCTCGGAAG CCACATCGTC CGTCCAGAAG 
GGCGAATCCC TGGCTGACTC CGTGCAGACC ATGAGCTGCT ATGCCGACGT CGTCGTGCTC 
CGGCACCCCC AGCCTGGAGC AGTGGAGCTG GCCGCCAAGC ACTGCCGGAG GCCAGTGATC 
AATGCTGGGG ATGGGGTCGG AGAGCACCCC ACCCAGGCCC TGCTGGACAT CTTCACCATC 
CGTGAGGAGC TGGGAACTGT CAATGGCATG ACGATCACGA TGGTGGGTGA CCTGAAGCAC 
GGACGCACAG TACATTCCCT GGCCTGCCTG CTCACCCAGT ATCGTGTCAG CCTGCGCTAC 
GTGGCACCTC CCAGCCTGCG CATGCCACCC ACTGTGCGGG CCTTCGTGGC CTCCCGCGGC 
ACCAAGCAGG AGGAATTCGA GAGCATTGAG GAGGCGCTGC CTGACACTGA TGTGCTCTAC 
ATGACTCGAA TCCAGAAGGA ACGATTTGGC TCTACCCAGG AGTACGAAGC TTGCTTTGGT 
CAGTTCATCC TCACTCCCCA CATCATGACC CGGGCCAAGA AGAAGATGGT GGTGATGCAC 
CCGATGCCCC GTGTCAACGA GATAAGCGTG GAAGTGGACT CGGATCCCCG CGCAGCCTAC 
TTCCGCCAGG CTGAGAACGG CATGTACATC CGCATGGCTC TGTTAGCCAC CGTGCTGGGC 
CGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_006712101.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712164.1 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.

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CloneID	OHu35603
Clone ID Related Accession (Same CDS sequence)	XM_006712101.2 , XM_006712101.3
Accession Version	XM_006712101.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	CAD protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGTATTACAT CATTGAAGTG 
AATGCCAGGC TCTCTCGCAG CTCTGCCCTG GCCAGTAAGG CCACAGGTTA TCCACTGGCT 
TATGTGGCAG CCAAGCTAGC ATTGGGCATC CCTTTGCCTG AGCTCAGGAA CTCTGTGACA 
GGGGGTACAG CAGCCTTTGA ACCCAGCGTG GATTATTGTG TGGTGAAGAT TCCTCGATGG 
GACCTTAGCA AGTTCCTGCG AGTCAGCACA AAGATTGGGA GCTGCATGAA GAGCGTTGGT 
GAAGTCATGG GCATTGGGCG TTCATTTGAG GAGGCCTTCC AGAAGGCCCT GCGCATGGTG 
GATGAGAACT GTGTGGGCTT TGATCACACA GTGAAACCAG TCAGCGATAT GGAGTTGGAG 
ACTCCAACAG ATAAGCGGAT TTTTGTGGTG GCAGCTGCTT TGTGGGCTGG TTATTCAGTG 
GACCGCCTGT ATGAGCTCAC ACGCATCGAC CGCTGGTTCC TGCACCGAAT GAAGCGTATC 
ATCGCACATG CCCAGCTGCT AGAACAACAC CGTGGACAGC CTTTGCCGCC AGACCTGCTG 
CAACAGGCCA AGTGTCTTGG CTTCTCAGAC AAACAGATTG CCCTTGCAGT TCTGAGCACA 
GAGCTGGCTG TTCGCAAGCT GCGTCAGGAA CTGGGGATCT GTCCAGCAGT GAAACAGATT 
GACACAGTTG CAGCTGAGTG GCCAGCCCAG ACAAATTACC TATACCTAAC GTATTGGGGC 
ACCACCCATG ACCTCACCTT TCGAACACCT CATGTCCTAG TCCTTGGCTC TGGCGTCTAC 
CGTATTGGCT CTAGCGTTGA ATTTGACTGG TGTGCTGTAG GCTGCATCCA GCAGCTCCGA 
AAGATGGGAT ATAAGACCAT CATGGTGAAC TATAACCCAG AGACAGTCAG CACCGACTAT 
GACATGTGTG ATCGACTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGACATCTAT 
GAGCTCGAGA ACCCTGAAGG TGTGATCCTA TCCATGGGTG GACAGCTGCC CAACAACATG 
GCCATGGCGT TGCATCGGCA GCAGTGCCGG GTGCTGGGCA CCTCCCCTGA AGCCATTGAC 
TCGGCTGAGA ACCGTTTCAA GTTTTCCCGG CTCCTTGACA CCATTGGTAT CAGCCAGCCT 
CAGTGGAGGG AGCTCAGTGA CCTCGAGTCT GCTCGCCAAT TCTGCCAGAC CGTGGGGTAC 
CCCTGTGTGG TGCGCCCCTC CTATGTGCTG AGCGGTGCTG CTATGAATGT GGCCTACACG 
GATGGAGACC TGGAGCGCTT CCTGAGCAGC GCAGCAGCCG TCTCCAAAGA GCATCCCGTG 
GTCATCTCCA AGTTCATCCA GGAGGCTAAG GAGATTGACG TGGATGCCGT GGCCTCTGAT 
GGTGTGGTGG CAGCCATCGC CATCTCTGAG CATGTGGAGA ATGCAGGTGT GCATTCAGGT 
GATGCGACGC TGGTGACCCC CCCACAAGAT ATCACTGCCA AAACCCTGGA GCGGATCAAA 
GCCATTGTGC ATGCTGTGGG CCAGGAGCTA CAGGTCACAG GACCCTTCAA TCTGCAGCTC 
ATTGCCAAGG ATGACCAGCT GAAAGTTATT GAATGCAACG TACGTGTCTC TCGCTCCTTC 
CCCTTCGTTT CCAAGACACT GGGTGTGGAC CTAGTAGCCT TGGCCACGCG GGTCATCATG 
GGGGAAGAAG TGGAACCTGT GGGGCTAATG ACTGGTTCTG GAGTCGTGGG AGTAAAGGTG 
CCTCAGTTCT CCTTCTCCCG CTTGGCGGGT GCTGACGTGG TGTTGGGTGT GGAAATGACC 
AGTACTGGGG AGGTGGCCGG CTTTGGGGAG AGCCGCTGTG AGGCATACCT CAAGGCCATG 
CTAAGCACTG GCTTTAAGAT CCCCAAGAAG AATATCCTGC TGACCATTGG CAGCTATAAG 
AACAAAAGCG AGCTGCTCCC AACTGTGCGG CTACTGGAGA GCCTGGGCTA CAGCCTCTAT 
GCCAGTCTCG GCACAGCTGA CTTCTACACT GAGCATGGCG TCAAGGTAAC AGCTGTGGAC 
TGGCACTTTG AGGAGGCTGT GGATGGTGAG TGCCCACCAC AGCGGAGCAT CCTGGAGCAG 
CTAGCTGAGA AAAACTTTGA GCTGGTGATT AACCTGTCAA TGCGTGGAGC TGGGGGCCGG 
CGTCTCTCTT CCTTTGTCAC CAAGGGCTAC CGCACCCGAC GCTTGGCCGC TGACTTCTCC 
GTGCCCCTAA TCATCGATAT CAAGTGCACC AAACTCTTTG TGGAGGCCCT AGGCCAGATC 
GGGCCAGCCC CTCCTTTGAA GGTGCATGTT GACTGTATGA CCTCCCAAAA GCTTGTGCGA 
CTGCCGGGAT TGATTGATGT CCATGTGCAC CTGCGGGAAC CAGGTGGGAC ACATAAGGAG 
GACTTTGCTT CAGGCACAGC CGCTGCCCTG GCTGGGGGTA TCACCATGGT GTGTGCCATG 
CCTAATACCC GGCCCCCCAT CATTGACGCC CCTGCTCTGG CCCTGGCCCA GAAGCTGGCA 
GAGGCTGGCG CCCGGTGCGA CTTTGCGCTA TTCCTTGGGG CCTCGTCTGA AAATGCAGGA 
ACCTTGGGCA CCGTGGCCGG GTCTGCAGCC GGGCTGAAGC TTTACCTCAA TGAGACCTTC 
TCTGAGCTGC GGCTGGACAG CGTGGTCCAG TGGATGGAGC ATTTCGAGAC ATGGCCCTCC 
CACCTCCCCA TTGTGGCTCA CGCAGAGCAG CAAACCGTGG CTGCTGTCCT CATGGTGGCT 
CAGCTCACTC AGCGCTCAGT GCACATATGT CACGTGGCAC GGAAGGAGGA GATCCTGCTA 
ATTAAAGCTG CAAAGGCACG GGGCTTGCCA GTGACCTGCG AGGTGGCTCC CCACCACCTG 
TTCCTAAGCC ATGATGACCT GGAGCGCCTG GGGCCTGGGA AGGGGGAGGT CCGGCCTGAG 
CTTGGCTCCC GCCAGGATGT GGAAGCCCTG TGGGAGAACA TGGCTGTCAT CGACTGCTTT 
GCCTCAGACC ATGCTCCCCA TACCTTGGAG GAGAAGTGTG GGTCCAGGCC CCCACCTGGG 
TTCCCAGGGT TAGAGACCAT GCTGCCACTA CTCCTGACGG CTGTAAGCGA GGGCCGGCTC 
AGCCTGGACG ACCTGCTGCA GCGATTGCAC CACAATCCTC GGCGCATCTT TCACCTGCCC 
CCGCAGGAGG ACACCTATGT GGAGGTGGAT CTGGAGCATG AGTGGACAAT TCCCAGCCAC 
ATGCCCTTCT CCAAGGCCCA CTGGACACCT TTTGAAGGGC AGAAAGTGAA GGGCACCGTC 
CGCCGTGTGG TCCTGCGAGG GGAGGTTGCC TATATCGATG GGCAGGTTCT GGTACCCCCG 
GGCTATGGAC AGGATGTACG GAAGTGGCCA CAGGGGGCTG TTCCTCAGCT CCCACCCTCA 
GCCCCTGCCA CTAGTGAGAT GACCACGACA CCTGAAAGAC CCCGCCGTGG CATCCCAGGG 
CTTCCTGATG GCCGCTTCCA TCTGCCGCCC CGAATCCATC GAGCCTCCGA CCCAGGTTTG 
CCAGCTGTGT TCCTCCGCCC AGGAGCTGGG ATCCCACGGG GCAGCAGAGC GTGGGCTGAG 
GAGCCAAAGG AGAAGTCCTC TCGGAAGGTA GCCGAGCCAG AGCTGATGGG AACCCCTGAT 
GGCACCTGCT ACCCTCCACC ACCAGTACCG AGACAGGCAT CTCCCCAGAA CCTGGGGACC 
CCTGGCTTGC TGCACCCCCA GACCTCACCC CTGCTGCACT CATTAGTGGG CCAACATATC 
CTGTCCGTCC AGCAGTTCAC CAAGGATCAG ATGTCTCACC TGTTCAATGT GGCACACACA 
CTGCGTATGA TGGTGCAGAA GGAGCGGAGC CTCGACATCC TGAAGGGGAA GGTCATGGCC 
TCCATGTTCT ATGAAGTGAG CACACGGACC AGCAGCTCCT TTGCAGCAGC CATGGCCCGG 
CTGGGAGGTG CTGTGCTCAG CTTCTCGGAA GCCACATCGT CCGTCCAGAA GGGCGAATCC 
CTGGCTGACT CCGTGCAGAC CATGAGCTGC TATGCCGACG TCGTCGTGCT CCGGCACCCC 
CAGCCTGGAG CAGTGGAGCT GGCCGCCAAG CACTGCCGGA GGCCAGTGAT CAATGCTGGG 
GATGGGGTCG GAGAGCACCC CACCCAGGCC CTGCTGGACA TCTTCACCAT CCGTGAGGAG 
CTGGGAACTG TCAATGGCAT GACGATCACG ATGGTGGGTG ACCTGAAGCA CGGACGCACA 
GTACATTCCC TGGCCTGCCT GCTCACCCAG TATCGTGTCA GCCTGCGCTA CGTGGCACCT 
CCCAGCCTGC GCATGCCACC CACTGTGCGG GCCTTCGTGG CCTCCCGCGG CACCAAGCAG 
GAGGAATTCG AGAGCATTGA GGAGGCGCTG CCTGACACTG ATGTGCTCTA CATGACTCGA 
ATCCAGAAGG AACGATTTGG CTCTACCCAG GAGTACGAAG CTTGCTTTGG TCAGTTCATC 
CTCACTCCCC ACATCATGAC CCGGGCCAAG AAGAAGATGG TGGTGATGCA CCCGATGCCC 
CGTGTCAACG AGATAAGCGT GGAAGTGGAC TCGGATCCCC GCGCAGCCTA CTTCCGCCAG 
GCTGAGAACG GCATGTACAT CCGCATGGCT CTGTTAGCCA CCGTGCTGGG CCGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712164.1
CDS	296..6835
Translation	MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAVFLRPGAGIPRGSRAWAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	XM_006712101.2
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712101.2

ORF Insert Sequence:

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGTATTACAT CATTGAAGTG 
AATGCCAGGC TCTCTCGCAG CTCTGCCCTG GCCAGTAAGG CCACAGGTTA TCCACTGGCT 
TATGTGGCAG CCAAGCTAGC ATTGGGCATC CCTTTGCCTG AGCTCAGGAA CTCTGTGACA 
GGGGGTACAG CAGCCTTTGA ACCCAGCGTG GATTATTGTG TGGTGAAGAT TCCTCGATGG 
GACCTTAGCA AGTTCCTGCG AGTCAGCACA AAGATTGGGA GCTGCATGAA GAGCGTTGGT 
GAAGTCATGG GCATTGGGCG TTCATTTGAG GAGGCCTTCC AGAAGGCCCT GCGCATGGTG 
GATGAGAACT GTGTGGGCTT TGATCACACA GTGAAACCAG TCAGCGATAT GGAGTTGGAG 
ACTCCAACAG ATAAGCGGAT TTTTGTGGTG GCAGCTGCTT TGTGGGCTGG TTATTCAGTG 
GACCGCCTGT ATGAGCTCAC ACGCATCGAC CGCTGGTTCC TGCACCGAAT GAAGCGTATC 
ATCGCACATG CCCAGCTGCT AGAACAACAC CGTGGACAGC CTTTGCCGCC AGACCTGCTG 
CAACAGGCCA AGTGTCTTGG CTTCTCAGAC AAACAGATTG CCCTTGCAGT TCTGAGCACA 
GAGCTGGCTG TTCGCAAGCT GCGTCAGGAA CTGGGGATCT GTCCAGCAGT GAAACAGATT 
GACACAGTTG CAGCTGAGTG GCCAGCCCAG ACAAATTACC TATACCTAAC GTATTGGGGC 
ACCACCCATG ACCTCACCTT TCGAACACCT CATGTCCTAG TCCTTGGCTC TGGCGTCTAC 
CGTATTGGCT CTAGCGTTGA ATTTGACTGG TGTGCTGTAG GCTGCATCCA GCAGCTCCGA 
AAGATGGGAT ATAAGACCAT CATGGTGAAC TATAACCCAG AGACAGTCAG CACCGACTAT 
GACATGTGTG ATCGACTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGACATCTAT 
GAGCTCGAGA ACCCTGAAGG TGTGATCCTA TCCATGGGTG GACAGCTGCC CAACAACATG 
GCCATGGCGT TGCATCGGCA GCAGTGCCGG GTGCTGGGCA CCTCCCCTGA AGCCATTGAC 
TCGGCTGAGA ACCGTTTCAA GTTTTCCCGG CTCCTTGACA CCATTGGTAT CAGCCAGCCT 
CAGTGGAGGG AGCTCAGTGA CCTCGAGTCT GCTCGCCAAT TCTGCCAGAC CGTGGGGTAC 
CCCTGTGTGG TGCGCCCCTC CTATGTGCTG AGCGGTGCTG CTATGAATGT GGCCTACACG 
GATGGAGACC TGGAGCGCTT CCTGAGCAGC GCAGCAGCCG TCTCCAAAGA GCATCCCGTG 
GTCATCTCCA AGTTCATCCA GGAGGCTAAG GAGATTGACG TGGATGCCGT GGCCTCTGAT 
GGTGTGGTGG CAGCCATCGC CATCTCTGAG CATGTGGAGA ATGCAGGTGT GCATTCAGGT 
GATGCGACGC TGGTGACCCC CCCACAAGAT ATCACTGCCA AAACCCTGGA GCGGATCAAA 
GCCATTGTGC ATGCTGTGGG CCAGGAGCTA CAGGTCACAG GACCCTTCAA TCTGCAGCTC 
ATTGCCAAGG ATGACCAGCT GAAAGTTATT GAATGCAACG TACGTGTCTC TCGCTCCTTC 
CCCTTCGTTT CCAAGACACT GGGTGTGGAC CTAGTAGCCT TGGCCACGCG GGTCATCATG 
GGGGAAGAAG TGGAACCTGT GGGGCTAATG ACTGGTTCTG GAGTCGTGGG AGTAAAGGTG 
CCTCAGTTCT CCTTCTCCCG CTTGGCGGGT GCTGACGTGG TGTTGGGTGT GGAAATGACC 
AGTACTGGGG AGGTGGCCGG CTTTGGGGAG AGCCGCTGTG AGGCATACCT CAAGGCCATG 
CTAAGCACTG GCTTTAAGAT CCCCAAGAAG AATATCCTGC TGACCATTGG CAGCTATAAG 
AACAAAAGCG AGCTGCTCCC AACTGTGCGG CTACTGGAGA GCCTGGGCTA CAGCCTCTAT 
GCCAGTCTCG GCACAGCTGA CTTCTACACT GAGCATGGCG TCAAGGTAAC AGCTGTGGAC 
TGGCACTTTG AGGAGGCTGT GGATGGTGAG TGCCCACCAC AGCGGAGCAT CCTGGAGCAG 
CTAGCTGAGA AAAACTTTGA GCTGGTGATT AACCTGTCAA TGCGTGGAGC TGGGGGCCGG 
CGTCTCTCTT CCTTTGTCAC CAAGGGCTAC CGCACCCGAC GCTTGGCCGC TGACTTCTCC 
GTGCCCCTAA TCATCGATAT CAAGTGCACC AAACTCTTTG TGGAGGCCCT AGGCCAGATC 
GGGCCAGCCC CTCCTTTGAA GGTGCATGTT GACTGTATGA CCTCCCAAAA GCTTGTGCGA 
CTGCCGGGAT TGATTGATGT CCATGTGCAC CTGCGGGAAC CAGGTGGGAC ACATAAGGAG 
GACTTTGCTT CAGGCACAGC CGCTGCCCTG GCTGGGGGTA TCACCATGGT GTGTGCCATG 
CCTAATACCC GGCCCCCCAT CATTGACGCC CCTGCTCTGG CCCTGGCCCA GAAGCTGGCA 
GAGGCTGGCG CCCGGTGCGA CTTTGCGCTA TTCCTTGGGG CCTCGTCTGA AAATGCAGGA 
ACCTTGGGCA CCGTGGCCGG GTCTGCAGCC GGGCTGAAGC TTTACCTCAA TGAGACCTTC 
TCTGAGCTGC GGCTGGACAG CGTGGTCCAG TGGATGGAGC ATTTCGAGAC ATGGCCCTCC 
CACCTCCCCA TTGTGGCTCA CGCAGAGCAG CAAACCGTGG CTGCTGTCCT CATGGTGGCT 
CAGCTCACTC AGCGCTCAGT GCACATATGT CACGTGGCAC GGAAGGAGGA GATCCTGCTA 
ATTAAAGCTG CAAAGGCACG GGGCTTGCCA GTGACCTGCG AGGTGGCTCC CCACCACCTG 
TTCCTAAGCC ATGATGACCT GGAGCGCCTG GGGCCTGGGA AGGGGGAGGT CCGGCCTGAG 
CTTGGCTCCC GCCAGGATGT GGAAGCCCTG TGGGAGAACA TGGCTGTCAT CGACTGCTTT 
GCCTCAGACC ATGCTCCCCA TACCTTGGAG GAGAAGTGTG GGTCCAGGCC CCCACCTGGG 
TTCCCAGGGT TAGAGACCAT GCTGCCACTA CTCCTGACGG CTGTAAGCGA GGGCCGGCTC 
AGCCTGGACG ACCTGCTGCA GCGATTGCAC CACAATCCTC GGCGCATCTT TCACCTGCCC 
CCGCAGGAGG ACACCTATGT GGAGGTGGAT CTGGAGCATG AGTGGACAAT TCCCAGCCAC 
ATGCCCTTCT CCAAGGCCCA CTGGACACCT TTTGAAGGGC AGAAAGTGAA GGGCACCGTC 
CGCCGTGTGG TCCTGCGAGG GGAGGTTGCC TATATCGATG GGCAGGTTCT GGTACCCCCG 
GGCTATGGAC AGGATGTACG GAAGTGGCCA CAGGGGGCTG TTCCTCAGCT CCCACCCTCA 
GCCCCTGCCA CTAGTGAGAT GACCACGACA CCTGAAAGAC CCCGCCGTGG CATCCCAGGG 
CTTCCTGATG GCCGCTTCCA TCTGCCGCCC CGAATCCATC GAGCCTCCGA CCCAGGTTTG 
CCAGCTGTGT TCCTCCGCCC AGGAGCTGGG ATCCCACGGG GCAGCAGAGC GTGGGCTGAG 
GAGCCAAAGG AGAAGTCCTC TCGGAAGGTA GCCGAGCCAG AGCTGATGGG AACCCCTGAT 
GGCACCTGCT ACCCTCCACC ACCAGTACCG AGACAGGCAT CTCCCCAGAA CCTGGGGACC 
CCTGGCTTGC TGCACCCCCA GACCTCACCC CTGCTGCACT CATTAGTGGG CCAACATATC 
CTGTCCGTCC AGCAGTTCAC CAAGGATCAG ATGTCTCACC TGTTCAATGT GGCACACACA 
CTGCGTATGA TGGTGCAGAA GGAGCGGAGC CTCGACATCC TGAAGGGGAA GGTCATGGCC 
TCCATGTTCT ATGAAGTGAG CACACGGACC AGCAGCTCCT TTGCAGCAGC CATGGCCCGG 
CTGGGAGGTG CTGTGCTCAG CTTCTCGGAA GCCACATCGT CCGTCCAGAA GGGCGAATCC 
CTGGCTGACT CCGTGCAGAC CATGAGCTGC TATGCCGACG TCGTCGTGCT CCGGCACCCC 
CAGCCTGGAG CAGTGGAGCT GGCCGCCAAG CACTGCCGGA GGCCAGTGAT CAATGCTGGG 
GATGGGGTCG GAGAGCACCC CACCCAGGCC CTGCTGGACA TCTTCACCAT CCGTGAGGAG 
CTGGGAACTG TCAATGGCAT GACGATCACG ATGGTGGGTG ACCTGAAGCA CGGACGCACA 
GTACATTCCC TGGCCTGCCT GCTCACCCAG TATCGTGTCA GCCTGCGCTA CGTGGCACCT 
CCCAGCCTGC GCATGCCACC CACTGTGCGG GCCTTCGTGG CCTCCCGCGG CACCAAGCAG 
GAGGAATTCG AGAGCATTGA GGAGGCGCTG CCTGACACTG ATGTGCTCTA CATGACTCGA 
ATCCAGAAGG AACGATTTGG CTCTACCCAG GAGTACGAAG CTTGCTTTGG TCAGTTCATC 
CTCACTCCCC ACATCATGAC CCGGGCCAAG AAGAAGATGG TGGTGATGCA CCCGATGCCC 
CGTGTCAACG AGATAAGCGT GGAAGTGGAC TCGGATCCCC GCGCAGCCTA CTTCCGCCAG 
GCTGAGAACG GCATGTACAT CCGCATGGCT CTGTTAGCCA CCGTGCTGGG CCGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAD ( XM_006712101.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712164.1 Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.

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CloneID	OHu35603
Clone ID Related Accession (Same CDS sequence)	XM_006712101.2 , XM_006712101.3
Accession Version	XM_006712101.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	CAD protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGTATTACAT CATTGAAGTG 
AATGCCAGGC TCTCTCGCAG CTCTGCCCTG GCCAGTAAGG CCACAGGTTA TCCACTGGCT 
TATGTGGCAG CCAAGCTAGC ATTGGGCATC CCTTTGCCTG AGCTCAGGAA CTCTGTGACA 
GGGGGTACAG CAGCCTTTGA ACCCAGCGTG GATTATTGTG TGGTGAAGAT TCCTCGATGG 
GACCTTAGCA AGTTCCTGCG AGTCAGCACA AAGATTGGGA GCTGCATGAA GAGCGTTGGT 
GAAGTCATGG GCATTGGGCG TTCATTTGAG GAGGCCTTCC AGAAGGCCCT GCGCATGGTG 
GATGAGAACT GTGTGGGCTT TGATCACACA GTGAAACCAG TCAGCGATAT GGAGTTGGAG 
ACTCCAACAG ATAAGCGGAT TTTTGTGGTG GCAGCTGCTT TGTGGGCTGG TTATTCAGTG 
GACCGCCTGT ATGAGCTCAC ACGCATCGAC CGCTGGTTCC TGCACCGAAT GAAGCGTATC 
ATCGCACATG CCCAGCTGCT AGAACAACAC CGTGGACAGC CTTTGCCGCC AGACCTGCTG 
CAACAGGCCA AGTGTCTTGG CTTCTCAGAC AAACAGATTG CCCTTGCAGT TCTGAGCACA 
GAGCTGGCTG TTCGCAAGCT GCGTCAGGAA CTGGGGATCT GTCCAGCAGT GAAACAGATT 
GACACAGTTG CAGCTGAGTG GCCAGCCCAG ACAAATTACC TATACCTAAC GTATTGGGGC 
ACCACCCATG ACCTCACCTT TCGAACACCT CATGTCCTAG TCCTTGGCTC TGGCGTCTAC 
CGTATTGGCT CTAGCGTTGA ATTTGACTGG TGTGCTGTAG GCTGCATCCA GCAGCTCCGA 
AAGATGGGAT ATAAGACCAT CATGGTGAAC TATAACCCAG AGACAGTCAG CACCGACTAT 
GACATGTGTG ATCGACTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGACATCTAT 
GAGCTCGAGA ACCCTGAAGG TGTGATCCTA TCCATGGGTG GACAGCTGCC CAACAACATG 
GCCATGGCGT TGCATCGGCA GCAGTGCCGG GTGCTGGGCA CCTCCCCTGA AGCCATTGAC 
TCGGCTGAGA ACCGTTTCAA GTTTTCCCGG CTCCTTGACA CCATTGGTAT CAGCCAGCCT 
CAGTGGAGGG AGCTCAGTGA CCTCGAGTCT GCTCGCCAAT TCTGCCAGAC CGTGGGGTAC 
CCCTGTGTGG TGCGCCCCTC CTATGTGCTG AGCGGTGCTG CTATGAATGT GGCCTACACG 
GATGGAGACC TGGAGCGCTT CCTGAGCAGC GCAGCAGCCG TCTCCAAAGA GCATCCCGTG 
GTCATCTCCA AGTTCATCCA GGAGGCTAAG GAGATTGACG TGGATGCCGT GGCCTCTGAT 
GGTGTGGTGG CAGCCATCGC CATCTCTGAG CATGTGGAGA ATGCAGGTGT GCATTCAGGT 
GATGCGACGC TGGTGACCCC CCCACAAGAT ATCACTGCCA AAACCCTGGA GCGGATCAAA 
GCCATTGTGC ATGCTGTGGG CCAGGAGCTA CAGGTCACAG GACCCTTCAA TCTGCAGCTC 
ATTGCCAAGG ATGACCAGCT GAAAGTTATT GAATGCAACG TACGTGTCTC TCGCTCCTTC 
CCCTTCGTTT CCAAGACACT GGGTGTGGAC CTAGTAGCCT TGGCCACGCG GGTCATCATG 
GGGGAAGAAG TGGAACCTGT GGGGCTAATG ACTGGTTCTG GAGTCGTGGG AGTAAAGGTG 
CCTCAGTTCT CCTTCTCCCG CTTGGCGGGT GCTGACGTGG TGTTGGGTGT GGAAATGACC 
AGTACTGGGG AGGTGGCCGG CTTTGGGGAG AGCCGCTGTG AGGCATACCT CAAGGCCATG 
CTAAGCACTG GCTTTAAGAT CCCCAAGAAG AATATCCTGC TGACCATTGG CAGCTATAAG 
AACAAAAGCG AGCTGCTCCC AACTGTGCGG CTACTGGAGA GCCTGGGCTA CAGCCTCTAT 
GCCAGTCTCG GCACAGCTGA CTTCTACACT GAGCATGGCG TCAAGGTAAC AGCTGTGGAC 
TGGCACTTTG AGGAGGCTGT GGATGGTGAG TGCCCACCAC AGCGGAGCAT CCTGGAGCAG 
CTAGCTGAGA AAAACTTTGA GCTGGTGATT AACCTGTCAA TGCGTGGAGC TGGGGGCCGG 
CGTCTCTCTT CCTTTGTCAC CAAGGGCTAC CGCACCCGAC GCTTGGCCGC TGACTTCTCC 
GTGCCCCTAA TCATCGATAT CAAGTGCACC AAACTCTTTG TGGAGGCCCT AGGCCAGATC 
GGGCCAGCCC CTCCTTTGAA GGTGCATGTT GACTGTATGA CCTCCCAAAA GCTTGTGCGA 
CTGCCGGGAT TGATTGATGT CCATGTGCAC CTGCGGGAAC CAGGTGGGAC ACATAAGGAG 
GACTTTGCTT CAGGCACAGC CGCTGCCCTG GCTGGGGGTA TCACCATGGT GTGTGCCATG 
CCTAATACCC GGCCCCCCAT CATTGACGCC CCTGCTCTGG CCCTGGCCCA GAAGCTGGCA 
GAGGCTGGCG CCCGGTGCGA CTTTGCGCTA TTCCTTGGGG CCTCGTCTGA AAATGCAGGA 
ACCTTGGGCA CCGTGGCCGG GTCTGCAGCC GGGCTGAAGC TTTACCTCAA TGAGACCTTC 
TCTGAGCTGC GGCTGGACAG CGTGGTCCAG TGGATGGAGC ATTTCGAGAC ATGGCCCTCC 
CACCTCCCCA TTGTGGCTCA CGCAGAGCAG CAAACCGTGG CTGCTGTCCT CATGGTGGCT 
CAGCTCACTC AGCGCTCAGT GCACATATGT CACGTGGCAC GGAAGGAGGA GATCCTGCTA 
ATTAAAGCTG CAAAGGCACG GGGCTTGCCA GTGACCTGCG AGGTGGCTCC CCACCACCTG 
TTCCTAAGCC ATGATGACCT GGAGCGCCTG GGGCCTGGGA AGGGGGAGGT CCGGCCTGAG 
CTTGGCTCCC GCCAGGATGT GGAAGCCCTG TGGGAGAACA TGGCTGTCAT CGACTGCTTT 
GCCTCAGACC ATGCTCCCCA TACCTTGGAG GAGAAGTGTG GGTCCAGGCC CCCACCTGGG 
TTCCCAGGGT TAGAGACCAT GCTGCCACTA CTCCTGACGG CTGTAAGCGA GGGCCGGCTC 
AGCCTGGACG ACCTGCTGCA GCGATTGCAC CACAATCCTC GGCGCATCTT TCACCTGCCC 
CCGCAGGAGG ACACCTATGT GGAGGTGGAT CTGGAGCATG AGTGGACAAT TCCCAGCCAC 
ATGCCCTTCT CCAAGGCCCA CTGGACACCT TTTGAAGGGC AGAAAGTGAA GGGCACCGTC 
CGCCGTGTGG TCCTGCGAGG GGAGGTTGCC TATATCGATG GGCAGGTTCT GGTACCCCCG 
GGCTATGGAC AGGATGTACG GAAGTGGCCA CAGGGGGCTG TTCCTCAGCT CCCACCCTCA 
GCCCCTGCCA CTAGTGAGAT GACCACGACA CCTGAAAGAC CCCGCCGTGG CATCCCAGGG 
CTTCCTGATG GCCGCTTCCA TCTGCCGCCC CGAATCCATC GAGCCTCCGA CCCAGGTTTG 
CCAGCTGTGT TCCTCCGCCC AGGAGCTGGG ATCCCACGGG GCAGCAGAGC GTGGGCTGAG 
GAGCCAAAGG AGAAGTCCTC TCGGAAGGTA GCCGAGCCAG AGCTGATGGG AACCCCTGAT 
GGCACCTGCT ACCCTCCACC ACCAGTACCG AGACAGGCAT CTCCCCAGAA CCTGGGGACC 
CCTGGCTTGC TGCACCCCCA GACCTCACCC CTGCTGCACT CATTAGTGGG CCAACATATC 
CTGTCCGTCC AGCAGTTCAC CAAGGATCAG ATGTCTCACC TGTTCAATGT GGCACACACA 
CTGCGTATGA TGGTGCAGAA GGAGCGGAGC CTCGACATCC TGAAGGGGAA GGTCATGGCC 
TCCATGTTCT ATGAAGTGAG CACACGGACC AGCAGCTCCT TTGCAGCAGC CATGGCCCGG 
CTGGGAGGTG CTGTGCTCAG CTTCTCGGAA GCCACATCGT CCGTCCAGAA GGGCGAATCC 
CTGGCTGACT CCGTGCAGAC CATGAGCTGC TATGCCGACG TCGTCGTGCT CCGGCACCCC 
CAGCCTGGAG CAGTGGAGCT GGCCGCCAAG CACTGCCGGA GGCCAGTGAT CAATGCTGGG 
GATGGGGTCG GAGAGCACCC CACCCAGGCC CTGCTGGACA TCTTCACCAT CCGTGAGGAG 
CTGGGAACTG TCAATGGCAT GACGATCACG ATGGTGGGTG ACCTGAAGCA CGGACGCACA 
GTACATTCCC TGGCCTGCCT GCTCACCCAG TATCGTGTCA GCCTGCGCTA CGTGGCACCT 
CCCAGCCTGC GCATGCCACC CACTGTGCGG GCCTTCGTGG CCTCCCGCGG CACCAAGCAG 
GAGGAATTCG AGAGCATTGA GGAGGCGCTG CCTGACACTG ATGTGCTCTA CATGACTCGA 
ATCCAGAAGG AACGATTTGG CTCTACCCAG GAGTACGAAG CTTGCTTTGG TCAGTTCATC 
CTCACTCCCC ACATCATGAC CCGGGCCAAG AAGAAGATGG TGGTGATGCA CCCGATGCCC 
CGTGTCAACG AGATAAGCGT GGAAGTGGAC TCGGATCCCC GCGCAGCCTA CTTCCGCCAG 
GCTGAGAACG GCATGTACAT CCGCATGGCT CTGTTAGCCA CCGTGCTGGG CCGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712164.1
CDS	47..6586
Translation	MAALVLEDGSVLRGQPFGAAVSTAGEVVFQTGMVGYPEALTDPSYKAQILVLTYPLIGNYGIPPDEMDEFGLCKWFESSGIHVAALVVGECCPTPSHWSATRTLHEWLQQHGIPGLQGVDTRELTKKLREQGSLLGKLVQNGTEPSSLPFLDPNARPLVPEVSIKTPRVFNTGGAPRILALDCGLKYNQIRCLCQRGAEVTVVPWDHALDSQEYEGLFLSNGPGDPASYPSVVSTLSRVLSEPNPRPVFGICLGHQLLALAIGAKTYKMRYGNRGHNQPCLLVGSGRCFLTSQNHGFAVETDSLPADWAPLFTNANDGSNEGIVHNSLPFFSVQFHPEHQAGPSDMELLFDIFLETVKEATAGNPGGQTVRERLTERLCPPGIPTPGSGLPPPRKVLILGSGGLSIGQAGEFDYSGSQAIKALKEENIQTLLINPNIATVQTSQGLADKVYFLPITPHYVTQVIRNERPDGVLLTFGGQTALNCGVELTKAGVLARYGVRVLGTPVETIELTEDRRAFAARMAEIGEHVAPSEAANSLEQAQAAAERLGYPVLVRAAFALGGLGSGFASNREELSALVAPAFAHTSQVLVDKSLKGWKEIEYEVVRDAYGNCVTYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGIPLPELRNSVTGGTAAFEPSVDYCVVKIPRWDLSKFLRVSTKIGSCMKSVGEVMGIGRSFEEAFQKALRMVDENCVGFDHTVKPVSDMELETPTDKRIFVVAAALWAGYSVDRLYELTRIDRWFLHRMKRIIAHAQLLEQHRGQPLPPDLLQQAKCLGFSDKQIALAVLSTELAVRKLRQELGICPAVKQIDTVAAEWPAQTNYLYLTYWGTTHDLTFRTPHVLVLGSGVYRIGSSVEFDWCAVGCIQQLRKMGYKTIMVNYNPETVSTDYDMCDRLYFDEISFEVVMDIYELENPEGVILSMGGQLPNNMAMALHRQQCRVLGTSPEAIDSAENRFKFSRLLDTIGISQPQWRELSDLESARQFCQTVGYPCVVRPSYVLSGAAMNVAYTDGDLERFLSSAAAVSKEHPVVISKFIQEAKEIDVDAVASDGVVAAIAISEHVENAGVHSGDATLVTPPQDITAKTLERIKAIVHAVGQELQVTGPFNLQLIAKDDQLKVIECNVRVSRSFPFVSKTLGVDLVALATRVIMGEEVEPVGLMTGSGVVGVKVPQFSFSRLAGADVVLGVEMTSTGEVAGFGESRCEAYLKAMLSTGFKIPKKNILLTIGSYKNKSELLPTVRLLESLGYSLYASLGTADFYTEHGVKVTAVDWHFEEAVDGECPPQRSILEQLAEKNFELVINLSMRGAGGRRLSSFVTKGYRTRRLAADFSVPLIIDIKCTKLFVEALGQIGPAPPLKVHVDCMTSQKLVRLPGLIDVHVHLREPGGTHKEDFASGTAAALAGGITMVCAMPNTRPPIIDAPALALAQKLAEAGARCDFALFLGASSENAGTLGTVAGSAAGLKLYLNETFSELRLDSVVQWMEHFETWPSHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGLPVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDCFASDHAPHTLEEKCGSRPPPGFPGLETMLPLLLTAVSEGRLSLDDLLQRLHHNPRRIFHLPPQEDTYVEVDLEHEWTIPSHMPFSKAHWTPFEGQKVKGTVRRVVLRGEVAYIDGQVLVPPGYGQDVRKWPQGAVPQLPPSAPATSEMTTTPERPRRGIPGLPDGRFHLPPRIHRASDPGLPAVFLRPGAGIPRGSRAWAEEPKEKSSRKVAEPELMGTPDGTCYPPPPVPRQASPQNLGTPGLLHPQTSPLLHSLVGQHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYADVVVLRHPQPGAVELAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVLGRF

Target ORF information:

RefSeq Version	XM_006712101.3
Organism	Homo sapiens(human)
Definition	Homo sapiens carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (CAD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712101.3

ORF Insert Sequence:

ATGGCGGCCC TAGTGTTGGA GGACGGGTCG GTCCTGCGGG GCCAGCCCTT TGGGGCCGCC 
GTGTCGACTG CCGGGGAAGT GGTGTTTCAA ACCGGCATGG TCGGCTACCC CGAGGCCCTC 
ACTGATCCCT CCTACAAGGC ACAGATCTTA GTGCTCACCT ATCCTCTGAT CGGCAACTAT 
GGCATCCCCC CAGATGAAAT GGATGAGTTC GGTCTCTGCA AGTGGTTTGA ATCCTCGGGC 
ATCCACGTAG CAGCACTGGT AGTGGGAGAG TGCTGTCCTA CTCCCAGCCA CTGGAGTGCC 
ACCCGCACCC TGCATGAGTG GCTGCAGCAG CATGGCATCC CTGGCTTGCA AGGAGTAGAC 
ACTCGGGAGC TGACCAAGAA GTTGCGGGAA CAGGGGTCTC TGCTGGGGAA GCTGGTCCAG 
AATGGAACAG AACCTTCATC CCTGCCATTC TTGGACCCCA ATGCCCGCCC CCTGGTACCA 
GAGGTCTCCA TTAAGACTCC ACGGGTATTC AATACAGGGG GTGCCCCTCG GATCCTTGCT 
TTGGACTGTG GCCTCAAGTA TAATCAGATC CGATGCCTCT GCCAGCGTGG GGCTGAGGTC 
ACTGTGGTAC CCTGGGACCA TGCACTAGAC AGCCAAGAGT ATGAGGGTCT CTTCTTAAGT 
AATGGGCCTG GTGACCCTGC CTCCTATCCC AGTGTCGTAT CCACACTGAG CCGTGTTTTA 
TCTGAGCCTA ATCCCCGACC TGTCTTTGGG ATCTGCCTGG GACACCAGCT ATTGGCCTTA 
GCCATTGGGG CCAAGACTTA CAAGATGAGA TATGGGAACC GAGGCCATAA CCAGCCCTGC 
TTGTTGGTGG GCTCTGGGCG CTGCTTTCTG ACATCCCAGA ACCATGGGTT TGCTGTGGAG 
ACAGACTCAC TGCCAGCAGA CTGGGCTCCT CTCTTCACCA ACGCCAATGA TGGTTCCAAT 
GAAGGCATTG TGCACAACAG CTTGCCTTTC TTCAGTGTCC AGTTTCACCC AGAGCACCAA 
GCTGGCCCTT CAGATATGGA ACTGCTTTTC GATATCTTTC TGGAAACTGT GAAAGAGGCC 
ACAGCTGGGA ACCCTGGGGG CCAGACAGTT AGAGAGCGGC TGACTGAGCG CCTCTGTCCC 
CCTGGGATTC CCACTCCCGG CTCTGGACTT CCACCACCAC GAAAGGTTCT GATCCTGGGC 
TCAGGGGGCC TCTCCATTGG CCAAGCTGGA GAATTTGACT ACTCGGGCTC TCAGGCAATT 
AAGGCCCTGA AGGAGGAAAA CATCCAGACG TTGCTGATCA ACCCCAATAT TGCCACAGTG 
CAGACCTCCC AGGGGCTGGC CGACAAGGTC TATTTTCTTC CCATAACACC TCATTATGTA 
ACCCAGGTGA TACGTAATGA ACGCCCCGAT GGTGTGTTAC TGACTTTTGG GGGCCAGACT 
GCTCTGAACT GTGGTGTGGA GCTGACCAAG GCCGGGGTGC TGGCTCGGTA TGGGGTCCGG 
GTCCTGGGCA CACCAGTGGA GACCATTGAG CTGACCGAGG ATCGACGGGC CTTTGCTGCC 
AGAATGGCAG AGATCGGAGA GCATGTGGCC CCGAGCGAGG CAGCAAATTC TCTTGAACAG 
GCCCAGGCAG CCGCTGAACG GCTGGGGTAC CCTGTGCTAG TGCGTGCAGC CTTTGCCCTG 
GGTGGCCTGG GCTCTGGCTT TGCCTCTAAC AGGGAGGAGC TCTCTGCTCT CGTGGCCCCA 
GCTTTTGCCC ATACCAGCCA AGTGCTAGTA GACAAGTCTC TGAAGGGATG GAAGGAGATT 
GAGTACGAGG TGGTGAGAGA CGCCTATGGC AACTGTGTCA CGTATTACAT CATTGAAGTG 
AATGCCAGGC TCTCTCGCAG CTCTGCCCTG GCCAGTAAGG CCACAGGTTA TCCACTGGCT 
TATGTGGCAG CCAAGCTAGC ATTGGGCATC CCTTTGCCTG AGCTCAGGAA CTCTGTGACA 
GGGGGTACAG CAGCCTTTGA ACCCAGCGTG GATTATTGTG TGGTGAAGAT TCCTCGATGG 
GACCTTAGCA AGTTCCTGCG AGTCAGCACA AAGATTGGGA GCTGCATGAA GAGCGTTGGT 
GAAGTCATGG GCATTGGGCG TTCATTTGAG GAGGCCTTCC AGAAGGCCCT GCGCATGGTG 
GATGAGAACT GTGTGGGCTT TGATCACACA GTGAAACCAG TCAGCGATAT GGAGTTGGAG 
ACTCCAACAG ATAAGCGGAT TTTTGTGGTG GCAGCTGCTT TGTGGGCTGG TTATTCAGTG 
GACCGCCTGT ATGAGCTCAC ACGCATCGAC CGCTGGTTCC TGCACCGAAT GAAGCGTATC 
ATCGCACATG CCCAGCTGCT AGAACAACAC CGTGGACAGC CTTTGCCGCC AGACCTGCTG 
CAACAGGCCA AGTGTCTTGG CTTCTCAGAC AAACAGATTG CCCTTGCAGT TCTGAGCACA 
GAGCTGGCTG TTCGCAAGCT GCGTCAGGAA CTGGGGATCT GTCCAGCAGT GAAACAGATT 
GACACAGTTG CAGCTGAGTG GCCAGCCCAG ACAAATTACC TATACCTAAC GTATTGGGGC 
ACCACCCATG ACCTCACCTT TCGAACACCT CATGTCCTAG TCCTTGGCTC TGGCGTCTAC 
CGTATTGGCT CTAGCGTTGA ATTTGACTGG TGTGCTGTAG GCTGCATCCA GCAGCTCCGA 
AAGATGGGAT ATAAGACCAT CATGGTGAAC TATAACCCAG AGACAGTCAG CACCGACTAT 
GACATGTGTG ATCGACTCTA CTTTGATGAG ATCTCTTTTG AGGTGGTGAT GGACATCTAT 
GAGCTCGAGA ACCCTGAAGG TGTGATCCTA TCCATGGGTG GACAGCTGCC CAACAACATG 
GCCATGGCGT TGCATCGGCA GCAGTGCCGG GTGCTGGGCA CCTCCCCTGA AGCCATTGAC 
TCGGCTGAGA ACCGTTTCAA GTTTTCCCGG CTCCTTGACA CCATTGGTAT CAGCCAGCCT 
CAGTGGAGGG AGCTCAGTGA CCTCGAGTCT GCTCGCCAAT TCTGCCAGAC CGTGGGGTAC 
CCCTGTGTGG TGCGCCCCTC CTATGTGCTG AGCGGTGCTG CTATGAATGT GGCCTACACG 
GATGGAGACC TGGAGCGCTT CCTGAGCAGC GCAGCAGCCG TCTCCAAAGA GCATCCCGTG 
GTCATCTCCA AGTTCATCCA GGAGGCTAAG GAGATTGACG TGGATGCCGT GGCCTCTGAT 
GGTGTGGTGG CAGCCATCGC CATCTCTGAG CATGTGGAGA ATGCAGGTGT GCATTCAGGT 
GATGCGACGC TGGTGACCCC CCCACAAGAT ATCACTGCCA AAACCCTGGA GCGGATCAAA 
GCCATTGTGC ATGCTGTGGG CCAGGAGCTA CAGGTCACAG GACCCTTCAA TCTGCAGCTC 
ATTGCCAAGG ATGACCAGCT GAAAGTTATT GAATGCAACG TACGTGTCTC TCGCTCCTTC 
CCCTTCGTTT CCAAGACACT GGGTGTGGAC CTAGTAGCCT TGGCCACGCG GGTCATCATG 
GGGGAAGAAG TGGAACCTGT GGGGCTAATG ACTGGTTCTG GAGTCGTGGG AGTAAAGGTG 
CCTCAGTTCT CCTTCTCCCG CTTGGCGGGT GCTGACGTGG TGTTGGGTGT GGAAATGACC 
AGTACTGGGG AGGTGGCCGG CTTTGGGGAG AGCCGCTGTG AGGCATACCT CAAGGCCATG 
CTAAGCACTG GCTTTAAGAT CCCCAAGAAG AATATCCTGC TGACCATTGG CAGCTATAAG 
AACAAAAGCG AGCTGCTCCC AACTGTGCGG CTACTGGAGA GCCTGGGCTA CAGCCTCTAT 
GCCAGTCTCG GCACAGCTGA CTTCTACACT GAGCATGGCG TCAAGGTAAC AGCTGTGGAC 
TGGCACTTTG AGGAGGCTGT GGATGGTGAG TGCCCACCAC AGCGGAGCAT CCTGGAGCAG 
CTAGCTGAGA AAAACTTTGA GCTGGTGATT AACCTGTCAA TGCGTGGAGC TGGGGGCCGG 
CGTCTCTCTT CCTTTGTCAC CAAGGGCTAC CGCACCCGAC GCTTGGCCGC TGACTTCTCC 
GTGCCCCTAA TCATCGATAT CAAGTGCACC AAACTCTTTG TGGAGGCCCT AGGCCAGATC 
GGGCCAGCCC CTCCTTTGAA GGTGCATGTT GACTGTATGA CCTCCCAAAA GCTTGTGCGA 
CTGCCGGGAT TGATTGATGT CCATGTGCAC CTGCGGGAAC CAGGTGGGAC ACATAAGGAG 
GACTTTGCTT CAGGCACAGC CGCTGCCCTG GCTGGGGGTA TCACCATGGT GTGTGCCATG 
CCTAATACCC GGCCCCCCAT CATTGACGCC CCTGCTCTGG CCCTGGCCCA GAAGCTGGCA 
GAGGCTGGCG CCCGGTGCGA CTTTGCGCTA TTCCTTGGGG CCTCGTCTGA AAATGCAGGA 
ACCTTGGGCA CCGTGGCCGG GTCTGCAGCC GGGCTGAAGC TTTACCTCAA TGAGACCTTC 
TCTGAGCTGC GGCTGGACAG CGTGGTCCAG TGGATGGAGC ATTTCGAGAC ATGGCCCTCC 
CACCTCCCCA TTGTGGCTCA CGCAGAGCAG CAAACCGTGG CTGCTGTCCT CATGGTGGCT 
CAGCTCACTC AGCGCTCAGT GCACATATGT CACGTGGCAC GGAAGGAGGA GATCCTGCTA 
ATTAAAGCTG CAAAGGCACG GGGCTTGCCA GTGACCTGCG AGGTGGCTCC CCACCACCTG 
TTCCTAAGCC ATGATGACCT GGAGCGCCTG GGGCCTGGGA AGGGGGAGGT CCGGCCTGAG 
CTTGGCTCCC GCCAGGATGT GGAAGCCCTG TGGGAGAACA TGGCTGTCAT CGACTGCTTT 
GCCTCAGACC ATGCTCCCCA TACCTTGGAG GAGAAGTGTG GGTCCAGGCC CCCACCTGGG 
TTCCCAGGGT TAGAGACCAT GCTGCCACTA CTCCTGACGG CTGTAAGCGA GGGCCGGCTC 
AGCCTGGACG ACCTGCTGCA GCGATTGCAC CACAATCCTC GGCGCATCTT TCACCTGCCC 
CCGCAGGAGG ACACCTATGT GGAGGTGGAT CTGGAGCATG AGTGGACAAT TCCCAGCCAC 
ATGCCCTTCT CCAAGGCCCA CTGGACACCT TTTGAAGGGC AGAAAGTGAA GGGCACCGTC 
CGCCGTGTGG TCCTGCGAGG GGAGGTTGCC TATATCGATG GGCAGGTTCT GGTACCCCCG 
GGCTATGGAC AGGATGTACG GAAGTGGCCA CAGGGGGCTG TTCCTCAGCT CCCACCCTCA 
GCCCCTGCCA CTAGTGAGAT GACCACGACA CCTGAAAGAC CCCGCCGTGG CATCCCAGGG 
CTTCCTGATG GCCGCTTCCA TCTGCCGCCC CGAATCCATC GAGCCTCCGA CCCAGGTTTG 
CCAGCTGTGT TCCTCCGCCC AGGAGCTGGG ATCCCACGGG GCAGCAGAGC GTGGGCTGAG 
GAGCCAAAGG AGAAGTCCTC TCGGAAGGTA GCCGAGCCAG AGCTGATGGG AACCCCTGAT 
GGCACCTGCT ACCCTCCACC ACCAGTACCG AGACAGGCAT CTCCCCAGAA CCTGGGGACC 
CCTGGCTTGC TGCACCCCCA GACCTCACCC CTGCTGCACT CATTAGTGGG CCAACATATC 
CTGTCCGTCC AGCAGTTCAC CAAGGATCAG ATGTCTCACC TGTTCAATGT GGCACACACA 
CTGCGTATGA TGGTGCAGAA GGAGCGGAGC CTCGACATCC TGAAGGGGAA GGTCATGGCC 
TCCATGTTCT ATGAAGTGAG CACACGGACC AGCAGCTCCT TTGCAGCAGC CATGGCCCGG 
CTGGGAGGTG CTGTGCTCAG CTTCTCGGAA GCCACATCGT CCGTCCAGAA GGGCGAATCC 
CTGGCTGACT CCGTGCAGAC CATGAGCTGC TATGCCGACG TCGTCGTGCT CCGGCACCCC 
CAGCCTGGAG CAGTGGAGCT GGCCGCCAAG CACTGCCGGA GGCCAGTGAT CAATGCTGGG 
GATGGGGTCG GAGAGCACCC CACCCAGGCC CTGCTGGACA TCTTCACCAT CCGTGAGGAG 
CTGGGAACTG TCAATGGCAT GACGATCACG ATGGTGGGTG ACCTGAAGCA CGGACGCACA 
GTACATTCCC TGGCCTGCCT GCTCACCCAG TATCGTGTCA GCCTGCGCTA CGTGGCACCT 
CCCAGCCTGC GCATGCCACC CACTGTGCGG GCCTTCGTGG CCTCCCGCGG CACCAAGCAG 
GAGGAATTCG AGAGCATTGA GGAGGCGCTG CCTGACACTG ATGTGCTCTA CATGACTCGA 
ATCCAGAAGG AACGATTTGG CTCTACCCAG GAGTACGAAG CTTGCTTTGG TCAGTTCATC 
CTCACTCCCC ACATCATGAC CCGGGCCAAG AAGAAGATGG TGGTGATGCA CCCGATGCCC 
CGTGTCAACG AGATAAGCGT GGAAGTGGAC TCGGATCCCC GCGCAGCCTA CTTCCGCCAG 
GCTGAGAACG GCATGTACAT CCGCATGGCT CTGTTAGCCA CCGTGCTGGG CCGTTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Charge neutralization in the active site of the catalytic trimer of aspartate transcarbamoylase promotes diverse structural changes.
Protein science : a publication of the Protein Society26(11)2221-2228(2017 Nov)
Endrizzi JA,Beernink PT

Structural Insight into the Core of CAD, the Multifunctional Protein Leading De Novo Pyrimidine Biosynthesis.
Structure (London, England : 1993)25(6)912-923.e5(2017 Jun)
Moreno-Morcillo M,Grande-Garc?a A,Ruiz-Ramos A,Del Ca?o-Ochoa F,Boskovic J,Ram?n-Maiques S

Tracking a CAD-ALK gene rearrangement in urine and blood of a colorectal cancer patient treated with an ALK inhibitor.
Annals of oncology : official journal of the European Society for Medical Oncology28(6)1302-1308(2017 Jun)
Siravegna G,Sartore-Bianchi A,Mussolin B,Cassingena A,Amatu A,Novara L,Buscarino M,Corti G,Crisafulli G,Bartolini A,Tosi F,Erlander M,Di Nicolantonio F,Siena S,Bardelli A

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CAD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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