r cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	r
Entrez Gene ID	32640
Full Name	rudimentary
Synonyms	ACT,CAD,CG18572,CG4601,CPS,DHO,DRORUD,Dmel\CG18572,GAT,PYR1,Su(b),csp2,fs(1)829,fs(1)M34,l(1)Ab
General protein information	Preferred Names rudimentary Names CG18572 gene product from transcript CG18572-RA CG18572-PA CG18572-PD CG18572-PE Suppressor-of-black carbamoylphosphate synthetase r-PA r-PD r-PE rudimentary gene suppressor of black
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14F5-15A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298414.1	NP_001285343.1	rudimentary, isoform D
NM_078653.2	NP_523377.1	rudimentary, isoform A
NM_206765.3	NP_996488.2	rudimentary, isoform E

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following r gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the r cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03045	NM_001298414.1 Latest version!	Drosophila melanogaster rudimentary (r), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03045	NM_206765.3 Latest version!	Drosophila melanogaster rudimentary (r), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa03045	NM_078653.2 Latest version!	Drosophila melanogaster rudimentary (r), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

r ( NM_001298414.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285343.1 Drosophila melanogaster rudimentary (r), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03045
Clone ID Related Accession (Same CDS sequence)	NM_001298414.1 , NM_078653.2 , NM_206765.3
Accession Version	NM_001298414.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rudimentary, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTA CGGACTGTTA CCTCGCACTG GAGGATGGCA CAGTGTTGCC CGGGTACTCC 
TTTGGCTACG TTCCCTCGGA GAATGAATCC AAAGTGGGTT TTGGTGGCGA GGTGGTCTTC 
CAAACGGGCA TGGTTGGCTA CACGGAGGCG CTCACGGATC GATCGTACAG TGGTCAGATC 
CTGGTGCTTA CCTATCCACT GATCGGTAAC TATGGGGTAC CAGCGCCGGA TGAGGACGAG 
CACGGACTGC CGCTGCACTT CGAGTGGATG AAAGGCGTCG TCCAGGCCAC CGCCTTGGTG 
GTGGGCGAGG TGGCTGAAGA GGCATTTCAC TGGCGCAAAT GGAAGACGCT GCCCGATTGG 
CTGAAGCAGC ACAAGGTGCC CGGAATTCAA GACATTGATA CTCGAGCCCT GACCAAAAAA 
CTGAGGGAGC AGGGAAGCAT GCTAGGCAAG ATTGTCTACG AGAAACCGCC GGTCGAAGGG 
CTGCCCAAGT CCAGTTTCGT AGATCCGAAC GTCAGGAATT TGGCCAAGGA GTGCAGCGTT 
AAGGAGCGAC AAGTGTACGG TAATCCCAAT GGCAAGGGAC CACGGATCGC CATTCTGGAC 
TGTGGACTGA AGCTCAACCA GTTGCGCTGC CTGCTGCAAC GTGGCGCCTC GGTGACCTTG 
CTCCCCTGGA GCGCTCGTCT CGAGGATGAG CAGTTCGACG CCCTCTTTCT GTCTAACGGA 
CCTGGTAATC CGGAGAGCTG CGACCAGATC GTCCAACAGG TGAGAAAAGT GATTGAGGAG 
GGTCAAAAGC CCGTCTTTGG AATATGTCTG GGTCACCAGC TGTTAGCGAA GGCAATCGGC 
TGCTCTACTT ACAAAATGAA GTACGGCAAT CGAGGACACA ATCTGCCCTG CCTGCACCGA 
GCAACTGGAC GTTGCCTGAT GACGTCGCAG AATCACGGAT ACGCGGTCGA TTTGGAGCAG 
TTGCCCGACG GCTGGTCGGA GCTATTTGTG AATGCCAACG ACGGCACCAA CGAGGGCATT 
GTCCATGCCA GCAAGCCCTA CTTTTCGGTT CAGTTCCATC CGGAGCATCA TGCTGGACCG 
CAGGACACGG AGTTCCTGTT CGATGTCTTT ATGGAGAGCA TTCAACAGAA GGATTTGACC 
ATTCCGCAGC TGATCGAGCA GCGACTGCGT CCGACTACGC CTGCCATAGA TTCGGCTCCA 
GTGATGCCGC GCAAAGTCCT AATCCTGGGA TCCGGTGGAC TGTCCATTGG TCAGGCTGGC 
GAGTTTGACT ACTCCGGATC GCAGGCCATC AAGGCCATGA GGGAATCAAA CATTCAAACA 
GTGCTCATCA ATCCGAACAT AGCCACCGTT CAGACCTCTA AGGGAATGGC CGACAAGTGC 
TACTTCCTTC CCCTAACACC TCACTATGTT GAGCAGGTGA TTAAGTCGGA GCGTCCGAAC 
GGAGTACTGC TCACCTTTGG CGGCCAGACC GCTCTCAATT GTGGCGTGCA ACTGGAGCGA 
GCCGGAGTTT TCTCCAAATA CAATGTCCGC ATTTTGGGCA CACCCATCCA GTCGATCATC 
GAGACGGAGG ATCGCAAGCT CTTTGCCGAG CGGGTCAACG AGATTGGCGA GCAGGTGGCG 
CCATCTGAGG CTGTGTACTC CGTTGCCCAG GCACTGGATG CGGCCAGTCG CTTGGGTTAT 
CCGGTGATGG CCCGTGCTGC CTTCTCCCTG GGCGGACTGG GTTCCGGGTT CGCCAACAAC 
GAGGAGGAGC TGCAGAGCCT GGCCCAACAG GCACTGGCCC ACTCGAGCCA ACTAATTGTG 
GACAAATCCC TGAAGGGTTG GAAGGAGGTG GAGTACGAGG TGGTGCGCGA TGCCTACAAC 
AACTGCATTA CCGTATGCAA CATGGAGAAC TTCGATCCCC TGGGCATCCA CACGGGCGAG 
AGTATTGTGG TGGCCCCATC GCAGACCCTC TCGGATCGCG AGTACCAAAT GCTCCGCAGC 
ACCGCCCTGA AGGTTATCCG TCACTTTGGT GTCGTTGGCG AGTGCAACAT TCAGTACGCC 
TTGTGTCCCC ATTCGGAGCA GTACTACATT ATCGAGGTGA ACGCCCGACT GTCGCGCAGC 
TCCGCGTTGG CCAGCAAGGC CACCGGCTAT CCACTGGCCT ATGTGGCCGC CAAGTTGGCT 
TTGGGATTGC CCCTGCCAGA TATCAAGAAC TCGGTGACGG GCAATACGAC GGCCTGCTTT 
GAGCCATCCC TCGATTACTG TGTGGTCAAG ATTCCACGCT GGGATTTGGC CAAATTCGTG 
CGGGTTAGCA AGCACATTGG CAGCTCGATG AAAAGTGTCG GTGAGGTGAT GGCCATCGGT 
CGCAACTTCG AGGAAGCGTT CCAAAAAGCC CTGCGCATGG TGGACAGCGA TGTGCTTGGC 
TTTGATCCGG ATGTGGTTCC GCTTAACAAG GAGCAGCTGG CCGAGCAGCT TTCGGAGCCG 
ACGGATCGTC GTCCCTTCGT CATTGCCGCT GCTTTGCAAT TGGGCATGAG CCTTAGGGAG 
CTGCATCAAC TAACCAATAT CGATTATTGG TTTCTGGAAA AGCTGGAAAG GATCATCTTA 
CTGCAGTCCC TGCTCACCCG TAATGGCAGT CGAACCGATG CCGCTCTGCT GTTGAAGGCC 
AAGCGTTTTG GTTTTTCGGA CAAGCAGATA GCTAAGTACA TTAAGAGCAC GGAGCTGGCT 
GTTCGTCACC AGCGGCAGGA GTTTGGCATC CGCCCGCATG TCAAGCAGAT CGACACGGTG 
GCCGGCGAAT GGCCCGCCAG TACCAACTAT CTGTACCACA CATATAACGG AAGCGAGCAC 
GATGTGGACT TCCCCGGCGG ACACACTATT GTGGTGGGCT CGGGCGTCTA TCGTATCGGA 
TCCTCAGTGG AGTTTGATTG GTGTGCCGTG GGCTGCCTGC GCGAATTGAG GAAGCTACAG 
CGACCCACCA TCATGATTAA CTACAATCCT GAAACGGTTT CCACCGACTA CGATATGTGC 
GATCGCTTGT ATTTCGAGGA GATTAGCTTC GAGGTGGTCA TGGACATCTA CGAGATGGAG 
AATAGCGAGG GCATCATTCT GTCCATGGGC GGCCAGCTGC CCAACAATAT AGCCATGGAT 
CTGCATCGCC AGCAGGCGAA GGTGTTGGGC ACATCGCCGG AGTCCATCGA CTGTGCCGAA 
AATCGATTTA AATTCTCGCG CATGCTGGAC AGGAAGGGTA TCTTGCAGCC ACGCTGGAAG 
GAGCTGACCA ACCTACAGTC GGCCATCGAA TTTTGCGAGG AGGTTGGCTA TCCCTGTCTG 
GTTAGGCCAT CCTATGTGCT CTCCGGTGCG GCCATGAATG TGGCCTATTC CAATCAGGAT 
CTGGAGACAT ATCTGAACGC CGCCTCCGAG GTGAGTCGCG AGCATCCGGT TGTCATTTCG 
AAGTTCCTCA CCGAGGCCAA GGAGATCGAT GTGGATGCAG TGGCATCCGA TGGACGCATC 
CTGTGCATGG CTGTTTCGGA GCACGTGGAG AATGCCGGAG TGCATTCAGG TGATGCCACG 
CTGGTTACCC CACCGCAGGA TCTGAATGCG GAGACCCTGG AGGCCATTAA ACGGATCACA 
TGCGATTTGG CCAGCGTACT GGATGTCACC GGTCCCTTCA ACATGCAGCT AATTGCCAAG 
AACAACGAGC TGAAGGTCAT CGAATGCAAT GTCCGTGTGT CGCGATCCTT CCCCTTCGTC 
TCCAAGACGC TGGACCACGA CTTTGTGGCC ACGGCGACGC GTGCGATTGT GGGACTCGAT 
GTGGAGCCAC TGGATGTGCT CCATGGCGTC GGCAAGGTGG GCGTCAAGGT GCCACAGTTC 
AGTTTCTCGC GGCTCGCGGG CGCGGATGTA CAGCTGGGCG TCGAGATGGC CTCCACCGGT 
GAGGTTGCCT GCTTCGGGGA CAACCGCTAT GAGGCGTATC TCAAGGCCAT GATGTCCACG 
GGCTTCCAGA TACCCAAGAA CGCCGTACTT CTCTCCATTG GCAGTTTCAA GCACAAGATG 
GAGCTGCTGC CTTCCATACG GGATCTGGCC AAGATGGGTT ACAAGCTATA CGCTTCCATG 
GGCACTGGCG ACTTTTATGC CGAACACGGA GTTAATGTGG AATCCGTGCA GTGGACATTC 
GACAAGACGA CTCCCGATGA CATCAACGGT GAGCTGAGAC ACCTGGCCGA GTTCCTGGCC 
AACAAGCAGT TCGACCTGGT CATCAACCTC CCGATGAGCG GCGGGGGTGC CAGGCGGGTC 
TCCTCGTTCA TGACCCATGG CTACCGCACC CGTCGCCTGG CCGTGGACTA CTCCATTCCG 
CTGGTGACCG ATGTGAAGTG CACCAAGCTT CTGGTAGAAT CAATGCGAAT GAACGGTGGC 
AAGCCGCCAA TGAAGACGCA CACGGATTGT ATGACCTCGC GTAGAATTGT GAAGCTGCCG 
GGCTTCATCG ATGTGCATGT GCATTTGCGG GAGCCGGGAG CCACGCACAA GGAGGACTTT 
GCCAGTGGAA CAGCAGCCGC TCTGGCTGGC GGCGTGACCC TCGTGTGCGC CATGCCCAAT 
ACAAATCCCT CGATCGTGGA CAGAGAGACG TTCACCCAGT TCCAGGAGCT GGCCAAAGCG 
GGAGCTCGAT GCGACTATGC CCTGTATGTG GGGGCCTCGG ATGACAATTG GGCGCAGGTC 
AACGAGCTGG CCAGTCATGC GTGTGGCCTT AAGATGTACT TAAACGACAC TTTCGGCACC 
CTAAAACTGA GCGACATGAC CAGCTGGCAG AGGCATCTCA GCCACTGGCC CAAGCGGTCT 
CCCATCGTTT GTCATGCGGA GAGGCAGAGC ACAGCGGCTG TGATTATGCT GGCCCATCTT 
CTCGACCGTT CGGTTCACAT CTGCCATGTG GCGCGAAAGG AGGAGATCCA ATTGATTCGT 
TCCGCCAAGG AAAAGGGCGT CAAGGTGACC TGCGAGGTGT GCCCCCATCA CTTGTTCCTC 
AGCACCAAGG ATGTGGAGCG TTTGGGTCAC GGAATGTCCG AGGTAAGGCC ATTGCTCTGC 
TCACCGGAGG ATCAAGAGGC TCTGTGGGAG AATATTGATT ACATCGATGT ATTTGCCACC 
GATCACGCGC CACACACACT GGCCGAGAAA CGTTCGGAGC GTCCACCGCC TGGATTTCCG 
GGCGTGGAGA CCATTCTGCC GCTCCTCCTG CAGGCTGTTC ACGAGGGTCG TTTGACGATG 
GAGGACATCA AGAGGAAGTT CCATCGCAAT CCAAAAATCA TCTTCAACCT GCCGGATCAG 
GCGCAGACCT ACGTGGAGGT GGATCTCGAC GAGGAGTGGA CCATAACGGG CAATGAGATG 
AAGAGCAAGT CCGGCTGGAC ACCATTTGAG GGCACCAAGG TCAAGGGACG CGTCCACCGG 
GTGGTTCTTC GCGGCGAGGT GGCCTTCGTC GATGGCCAAG TGTTGGTGCA GCCCGGCTTC 
GGGCAGAATG TGCGCCCCAA ACAGAGTCCA TTGGCGTCGG AGGCATCACA GGATCTGCTG 
CCCAGCGACA ACGATGCCAA TGACACCTTT ACCCGCCTCC TCACCTCCGA AGGACCCGGT 
GGAGGCGTAC ATGGAATATC GACCAAGGTG CACTTTGTGG ACGGAGCCAA CTTCCTGCGC 
CCCAACTCGC CATCCCCACG CATCCGTCTG GATTCCGCCA GCAACACCAC GCTGCGGGAA 
TACTTGCAGC GCACTACCAA CTCAAATCCG GTGGCCCACT CGCTAATGGG CAAGCACATC 
CTTGCCGTTG ACATGTTCAA CAAAGATCAC CTCAACGACA TCTTTAACCT GGCACAGCTG 
CTCAAGCTGC GGGGTACGAA GGATCGTCCG GTGGACGAGC TGCTGCCAGG CAAGATCATG 
GCCAGTGTGT TTTACGAGGT CAGCACGCGG ACGCAGTGCA GCTTTGCAGC TGCCATGCTG 
CGTTTGGGAG GGCGAGTGAT CAGCATGGAC AACATCACGT CGTCGGTGAA GAAGGGCGAG 
AGCCTGGAGG ACAGCATCAA GGTTGTGTCC AGCTATGCCG ACGTCGTGGT GCTCCGTCAT 
CCATCGCCCG GAGCTGTAGC GCGCGCAGCG ACCTTCTCCC GAAAGCCGCT TATCAATGCC 
GGCGATGGCG TCGGTGAGCA TCCCACGCAG GCGCTGCTGG ACATCTTCAC CATCCGCGAG 
GAGTTTGGCA CTGTGAACGG GCTGACCATC ACCATGGTGG GCGACTTGAA GAACGGACGG 
ACCGTGCACT CGCTGGCCCG CCTGCTGACC CTGTACAATG TGAACCTGCA GTATGTGGCG 
CCGAATAGCC TGCAGATGCC CGATGAGGTC GTCCAGTTCG TCCATCAGCG CGGCGTCAAG 
CAGTTATTTG CACGCGACCT AAAGAATGTG CTGCCCGACA CGGATGTGCT CTACATGACT 
CGCATTCAGC GCGAGCGTTT TGATAACGTG GAAGATTACG AGAAGTGCTG TGGCCATCTG 
GTGTTGACGC CCGAGCATAT GATGCGGGCC AAGAAGCGTT CGATTGTTCT GCATCCGCTG 
CCGCGTCTGA ACGAGATCAG CCGGGAGATC GACTCGGACC CAAGGGCCGC CTACTTCCGG 
CAGGCGGAGT ACGGCATGTA CATCCGTATG GCCTTGCTGG CCATGGTCGT TGGTGGACGC 
AATACGGCGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285343.1
CDS	83..6757
Translation	MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQILVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDWLKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSVKERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNGPGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHRATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGPQDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAGEFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPNGVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVAPSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIVDKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRSTALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIGRNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRELHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELAVRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIGSSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEMENSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWKELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVISKFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRITCDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLDVEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMSTGFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVESVQWTFDKTTPDDINGELRHLAEFLANKQFDLVINLPMSGGGARRVSSFMTHGYRTRRLAVDYSIPLVTDVKCTKLLVESMRMNGGKPPMKTHTDCMTSRRIVKLPGFIDVHVHLREPGATHKEDFASGTAAALAGGVTLVCAMPNTNPSIVDRETFTQFQELAKAGARCDYALYVGASDDNWAQVNELASHACGLKMYLNDTFGTLKLSDMTSWQRHLSHWPKRSPIVCHAERQSTAAVIMLAHLLDRSVHICHVARKEEIQLIRSAKEKGVKVTCEVCPHHLFLSTKDVERLGHGMSEVRPLLCSPEDQEALWENIDYIDVFATDHAPHTLAEKRSERPPPGFPGVETILPLLLQAVHEGRLTMEDIKRKFHRNPKIIFNLPDQAQTYVEVDLDEEWTITGNEMKSKSGWTPFEGTKVKGRVHRVVLRGEVAFVDGQVLVQPGFGQNVRPKQSPLASEASQDLLPSDNDANDTFTRLLTSEGPGGGVHGISTKVHFVDGANFLRPNSPSPRIRLDSASNTTLREYLQRTTNSNPVAHSLMGKHILAVDMFNKDHLNDIFNLAQLLKLRGTKDRPVDELLPGKIMASVFYEVSTRTQCSFAAAMLRLGGRVISMDNITSSVKKGESLEDSIKVVSSYADVVVLRHPSPGAVARAATFSRKPLINAGDGVGEHPTQALLDIFTIREEFGTVNGLTITMVGDLKNGRTVHSLARLLTLYNVNLQYVAPNSLQMPDEVVQFVHQRGVKQLFARDLKNVLPDTDVLYMTRIQRERFDNVEDYEKCCGHLVLTPEHMMRAKKRSIVLHPLPRLNEISREIDSDPRAAYFRQAEYGMYIRMALLAMVVGGRNTAL

Target ORF information:

RefSeq Version	NM_001298414.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rudimentary (r), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298414.1

ORF Insert Sequence:

ATGGCCTCTA CGGACTGTTA CCTCGCACTG GAGGATGGCA CAGTGTTGCC CGGGTACTCC 
TTTGGCTACG TTCCCTCGGA GAATGAATCC AAAGTGGGTT TTGGTGGCGA GGTGGTCTTC 
CAAACGGGCA TGGTTGGCTA CACGGAGGCG CTCACGGATC GATCGTACAG TGGTCAGATC 
CTGGTGCTTA CCTATCCACT GATCGGTAAC TATGGGGTAC CAGCGCCGGA TGAGGACGAG 
CACGGACTGC CGCTGCACTT CGAGTGGATG AAAGGCGTCG TCCAGGCCAC CGCCTTGGTG 
GTGGGCGAGG TGGCTGAAGA GGCATTTCAC TGGCGCAAAT GGAAGACGCT GCCCGATTGG 
CTGAAGCAGC ACAAGGTGCC CGGAATTCAA GACATTGATA CTCGAGCCCT GACCAAAAAA 
CTGAGGGAGC AGGGAAGCAT GCTAGGCAAG ATTGTCTACG AGAAACCGCC GGTCGAAGGG 
CTGCCCAAGT CCAGTTTCGT AGATCCGAAC GTCAGGAATT TGGCCAAGGA GTGCAGCGTT 
AAGGAGCGAC AAGTGTACGG TAATCCCAAT GGCAAGGGAC CACGGATCGC CATTCTGGAC 
TGTGGACTGA AGCTCAACCA GTTGCGCTGC CTGCTGCAAC GTGGCGCCTC GGTGACCTTG 
CTCCCCTGGA GCGCTCGTCT CGAGGATGAG CAGTTCGACG CCCTCTTTCT GTCTAACGGA 
CCTGGTAATC CGGAGAGCTG CGACCAGATC GTCCAACAGG TGAGAAAAGT GATTGAGGAG 
GGTCAAAAGC CCGTCTTTGG AATATGTCTG GGTCACCAGC TGTTAGCGAA GGCAATCGGC 
TGCTCTACTT ACAAAATGAA GTACGGCAAT CGAGGACACA ATCTGCCCTG CCTGCACCGA 
GCAACTGGAC GTTGCCTGAT GACGTCGCAG AATCACGGAT ACGCGGTCGA TTTGGAGCAG 
TTGCCCGACG GCTGGTCGGA GCTATTTGTG AATGCCAACG ACGGCACCAA CGAGGGCATT 
GTCCATGCCA GCAAGCCCTA CTTTTCGGTT CAGTTCCATC CGGAGCATCA TGCTGGACCG 
CAGGACACGG AGTTCCTGTT CGATGTCTTT ATGGAGAGCA TTCAACAGAA GGATTTGACC 
ATTCCGCAGC TGATCGAGCA GCGACTGCGT CCGACTACGC CTGCCATAGA TTCGGCTCCA 
GTGATGCCGC GCAAAGTCCT AATCCTGGGA TCCGGTGGAC TGTCCATTGG TCAGGCTGGC 
GAGTTTGACT ACTCCGGATC GCAGGCCATC AAGGCCATGA GGGAATCAAA CATTCAAACA 
GTGCTCATCA ATCCGAACAT AGCCACCGTT CAGACCTCTA AGGGAATGGC CGACAAGTGC 
TACTTCCTTC CCCTAACACC TCACTATGTT GAGCAGGTGA TTAAGTCGGA GCGTCCGAAC 
GGAGTACTGC TCACCTTTGG CGGCCAGACC GCTCTCAATT GTGGCGTGCA ACTGGAGCGA 
GCCGGAGTTT TCTCCAAATA CAATGTCCGC ATTTTGGGCA CACCCATCCA GTCGATCATC 
GAGACGGAGG ATCGCAAGCT CTTTGCCGAG CGGGTCAACG AGATTGGCGA GCAGGTGGCG 
CCATCTGAGG CTGTGTACTC CGTTGCCCAG GCACTGGATG CGGCCAGTCG CTTGGGTTAT 
CCGGTGATGG CCCGTGCTGC CTTCTCCCTG GGCGGACTGG GTTCCGGGTT CGCCAACAAC 
GAGGAGGAGC TGCAGAGCCT GGCCCAACAG GCACTGGCCC ACTCGAGCCA ACTAATTGTG 
GACAAATCCC TGAAGGGTTG GAAGGAGGTG GAGTACGAGG TGGTGCGCGA TGCCTACAAC 
AACTGCATTA CCGTATGCAA CATGGAGAAC TTCGATCCCC TGGGCATCCA CACGGGCGAG 
AGTATTGTGG TGGCCCCATC GCAGACCCTC TCGGATCGCG AGTACCAAAT GCTCCGCAGC 
ACCGCCCTGA AGGTTATCCG TCACTTTGGT GTCGTTGGCG AGTGCAACAT TCAGTACGCC 
TTGTGTCCCC ATTCGGAGCA GTACTACATT ATCGAGGTGA ACGCCCGACT GTCGCGCAGC 
TCCGCGTTGG CCAGCAAGGC CACCGGCTAT CCACTGGCCT ATGTGGCCGC CAAGTTGGCT 
TTGGGATTGC CCCTGCCAGA TATCAAGAAC TCGGTGACGG GCAATACGAC GGCCTGCTTT 
GAGCCATCCC TCGATTACTG TGTGGTCAAG ATTCCACGCT GGGATTTGGC CAAATTCGTG 
CGGGTTAGCA AGCACATTGG CAGCTCGATG AAAAGTGTCG GTGAGGTGAT GGCCATCGGT 
CGCAACTTCG AGGAAGCGTT CCAAAAAGCC CTGCGCATGG TGGACAGCGA TGTGCTTGGC 
TTTGATCCGG ATGTGGTTCC GCTTAACAAG GAGCAGCTGG CCGAGCAGCT TTCGGAGCCG 
ACGGATCGTC GTCCCTTCGT CATTGCCGCT GCTTTGCAAT TGGGCATGAG CCTTAGGGAG 
CTGCATCAAC TAACCAATAT CGATTATTGG TTTCTGGAAA AGCTGGAAAG GATCATCTTA 
CTGCAGTCCC TGCTCACCCG TAATGGCAGT CGAACCGATG CCGCTCTGCT GTTGAAGGCC 
AAGCGTTTTG GTTTTTCGGA CAAGCAGATA GCTAAGTACA TTAAGAGCAC GGAGCTGGCT 
GTTCGTCACC AGCGGCAGGA GTTTGGCATC CGCCCGCATG TCAAGCAGAT CGACACGGTG 
GCCGGCGAAT GGCCCGCCAG TACCAACTAT CTGTACCACA CATATAACGG AAGCGAGCAC 
GATGTGGACT TCCCCGGCGG ACACACTATT GTGGTGGGCT CGGGCGTCTA TCGTATCGGA 
TCCTCAGTGG AGTTTGATTG GTGTGCCGTG GGCTGCCTGC GCGAATTGAG GAAGCTACAG 
CGACCCACCA TCATGATTAA CTACAATCCT GAAACGGTTT CCACCGACTA CGATATGTGC 
GATCGCTTGT ATTTCGAGGA GATTAGCTTC GAGGTGGTCA TGGACATCTA CGAGATGGAG 
AATAGCGAGG GCATCATTCT GTCCATGGGC GGCCAGCTGC CCAACAATAT AGCCATGGAT 
CTGCATCGCC AGCAGGCGAA GGTGTTGGGC ACATCGCCGG AGTCCATCGA CTGTGCCGAA 
AATCGATTTA AATTCTCGCG CATGCTGGAC AGGAAGGGTA TCTTGCAGCC ACGCTGGAAG 
GAGCTGACCA ACCTACAGTC GGCCATCGAA TTTTGCGAGG AGGTTGGCTA TCCCTGTCTG 
GTTAGGCCAT CCTATGTGCT CTCCGGTGCG GCCATGAATG TGGCCTATTC CAATCAGGAT 
CTGGAGACAT ATCTGAACGC CGCCTCCGAG GTGAGTCGCG AGCATCCGGT TGTCATTTCG 
AAGTTCCTCA CCGAGGCCAA GGAGATCGAT GTGGATGCAG TGGCATCCGA TGGACGCATC 
CTGTGCATGG CTGTTTCGGA GCACGTGGAG AATGCCGGAG TGCATTCAGG TGATGCCACG 
CTGGTTACCC CACCGCAGGA TCTGAATGCG GAGACCCTGG AGGCCATTAA ACGGATCACA 
TGCGATTTGG CCAGCGTACT GGATGTCACC GGTCCCTTCA ACATGCAGCT AATTGCCAAG 
AACAACGAGC TGAAGGTCAT CGAATGCAAT GTCCGTGTGT CGCGATCCTT CCCCTTCGTC 
TCCAAGACGC TGGACCACGA CTTTGTGGCC ACGGCGACGC GTGCGATTGT GGGACTCGAT 
GTGGAGCCAC TGGATGTGCT CCATGGCGTC GGCAAGGTGG GCGTCAAGGT GCCACAGTTC 
AGTTTCTCGC GGCTCGCGGG CGCGGATGTA CAGCTGGGCG TCGAGATGGC CTCCACCGGT 
GAGGTTGCCT GCTTCGGGGA CAACCGCTAT GAGGCGTATC TCAAGGCCAT GATGTCCACG 
GGCTTCCAGA TACCCAAGAA CGCCGTACTT CTCTCCATTG GCAGTTTCAA GCACAAGATG 
GAGCTGCTGC CTTCCATACG GGATCTGGCC AAGATGGGTT ACAAGCTATA CGCTTCCATG 
GGCACTGGCG ACTTTTATGC CGAACACGGA GTTAATGTGG AATCCGTGCA GTGGACATTC 
GACAAGACGA CTCCCGATGA CATCAACGGT GAGCTGAGAC ACCTGGCCGA GTTCCTGGCC 
AACAAGCAGT TCGACCTGGT CATCAACCTC CCGATGAGCG GCGGGGGTGC CAGGCGGGTC 
TCCTCGTTCA TGACCCATGG CTACCGCACC CGTCGCCTGG CCGTGGACTA CTCCATTCCG 
CTGGTGACCG ATGTGAAGTG CACCAAGCTT CTGGTAGAAT CAATGCGAAT GAACGGTGGC 
AAGCCGCCAA TGAAGACGCA CACGGATTGT ATGACCTCGC GTAGAATTGT GAAGCTGCCG 
GGCTTCATCG ATGTGCATGT GCATTTGCGG GAGCCGGGAG CCACGCACAA GGAGGACTTT 
GCCAGTGGAA CAGCAGCCGC TCTGGCTGGC GGCGTGACCC TCGTGTGCGC CATGCCCAAT 
ACAAATCCCT CGATCGTGGA CAGAGAGACG TTCACCCAGT TCCAGGAGCT GGCCAAAGCG 
GGAGCTCGAT GCGACTATGC CCTGTATGTG GGGGCCTCGG ATGACAATTG GGCGCAGGTC 
AACGAGCTGG CCAGTCATGC GTGTGGCCTT AAGATGTACT TAAACGACAC TTTCGGCACC 
CTAAAACTGA GCGACATGAC CAGCTGGCAG AGGCATCTCA GCCACTGGCC CAAGCGGTCT 
CCCATCGTTT GTCATGCGGA GAGGCAGAGC ACAGCGGCTG TGATTATGCT GGCCCATCTT 
CTCGACCGTT CGGTTCACAT CTGCCATGTG GCGCGAAAGG AGGAGATCCA ATTGATTCGT 
TCCGCCAAGG AAAAGGGCGT CAAGGTGACC TGCGAGGTGT GCCCCCATCA CTTGTTCCTC 
AGCACCAAGG ATGTGGAGCG TTTGGGTCAC GGAATGTCCG AGGTAAGGCC ATTGCTCTGC 
TCACCGGAGG ATCAAGAGGC TCTGTGGGAG AATATTGATT ACATCGATGT ATTTGCCACC 
GATCACGCGC CACACACACT GGCCGAGAAA CGTTCGGAGC GTCCACCGCC TGGATTTCCG 
GGCGTGGAGA CCATTCTGCC GCTCCTCCTG CAGGCTGTTC ACGAGGGTCG TTTGACGATG 
GAGGACATCA AGAGGAAGTT CCATCGCAAT CCAAAAATCA TCTTCAACCT GCCGGATCAG 
GCGCAGACCT ACGTGGAGGT GGATCTCGAC GAGGAGTGGA CCATAACGGG CAATGAGATG 
AAGAGCAAGT CCGGCTGGAC ACCATTTGAG GGCACCAAGG TCAAGGGACG CGTCCACCGG 
GTGGTTCTTC GCGGCGAGGT GGCCTTCGTC GATGGCCAAG TGTTGGTGCA GCCCGGCTTC 
GGGCAGAATG TGCGCCCCAA ACAGAGTCCA TTGGCGTCGG AGGCATCACA GGATCTGCTG 
CCCAGCGACA ACGATGCCAA TGACACCTTT ACCCGCCTCC TCACCTCCGA AGGACCCGGT 
GGAGGCGTAC ATGGAATATC GACCAAGGTG CACTTTGTGG ACGGAGCCAA CTTCCTGCGC 
CCCAACTCGC CATCCCCACG CATCCGTCTG GATTCCGCCA GCAACACCAC GCTGCGGGAA 
TACTTGCAGC GCACTACCAA CTCAAATCCG GTGGCCCACT CGCTAATGGG CAAGCACATC 
CTTGCCGTTG ACATGTTCAA CAAAGATCAC CTCAACGACA TCTTTAACCT GGCACAGCTG 
CTCAAGCTGC GGGGTACGAA GGATCGTCCG GTGGACGAGC TGCTGCCAGG CAAGATCATG 
GCCAGTGTGT TTTACGAGGT CAGCACGCGG ACGCAGTGCA GCTTTGCAGC TGCCATGCTG 
CGTTTGGGAG GGCGAGTGAT CAGCATGGAC AACATCACGT CGTCGGTGAA GAAGGGCGAG 
AGCCTGGAGG ACAGCATCAA GGTTGTGTCC AGCTATGCCG ACGTCGTGGT GCTCCGTCAT 
CCATCGCCCG GAGCTGTAGC GCGCGCAGCG ACCTTCTCCC GAAAGCCGCT TATCAATGCC 
GGCGATGGCG TCGGTGAGCA TCCCACGCAG GCGCTGCTGG ACATCTTCAC CATCCGCGAG 
GAGTTTGGCA CTGTGAACGG GCTGACCATC ACCATGGTGG GCGACTTGAA GAACGGACGG 
ACCGTGCACT CGCTGGCCCG CCTGCTGACC CTGTACAATG TGAACCTGCA GTATGTGGCG 
CCGAATAGCC TGCAGATGCC CGATGAGGTC GTCCAGTTCG TCCATCAGCG CGGCGTCAAG 
CAGTTATTTG CACGCGACCT AAAGAATGTG CTGCCCGACA CGGATGTGCT CTACATGACT 
CGCATTCAGC GCGAGCGTTT TGATAACGTG GAAGATTACG AGAAGTGCTG TGGCCATCTG 
GTGTTGACGC CCGAGCATAT GATGCGGGCC AAGAAGCGTT CGATTGTTCT GCATCCGCTG 
CCGCGTCTGA ACGAGATCAG CCGGGAGATC GACTCGGACC CAAGGGCCGC CTACTTCCGG 
CAGGCGGAGT ACGGCATGTA CATCCGTATG GCCTTGCTGG CCATGGTCGT TGGTGGACGC 
AATACGGCGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

r ( NM_078653.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523377.1 Drosophila melanogaster rudimentary (r), transcript variant A, mRNA.

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CloneID	OFa03045
Clone ID Related Accession (Same CDS sequence)	NM_001298414.1 , NM_078653.2 , NM_206765.3
Accession Version	NM_078653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rudimentary, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078653.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTA CGGACTGTTA CCTCGCACTG GAGGATGGCA CAGTGTTGCC CGGGTACTCC 
TTTGGCTACG TTCCCTCGGA GAATGAATCC AAAGTGGGTT TTGGTGGCGA GGTGGTCTTC 
CAAACGGGCA TGGTTGGCTA CACGGAGGCG CTCACGGATC GATCGTACAG TGGTCAGATC 
CTGGTGCTTA CCTATCCACT GATCGGTAAC TATGGGGTAC CAGCGCCGGA TGAGGACGAG 
CACGGACTGC CGCTGCACTT CGAGTGGATG AAAGGCGTCG TCCAGGCCAC CGCCTTGGTG 
GTGGGCGAGG TGGCTGAAGA GGCATTTCAC TGGCGCAAAT GGAAGACGCT GCCCGATTGG 
CTGAAGCAGC ACAAGGTGCC CGGAATTCAA GACATTGATA CTCGAGCCCT GACCAAAAAA 
CTGAGGGAGC AGGGAAGCAT GCTAGGCAAG ATTGTCTACG AGAAACCGCC GGTCGAAGGG 
CTGCCCAAGT CCAGTTTCGT AGATCCGAAC GTCAGGAATT TGGCCAAGGA GTGCAGCGTT 
AAGGAGCGAC AAGTGTACGG TAATCCCAAT GGCAAGGGAC CACGGATCGC CATTCTGGAC 
TGTGGACTGA AGCTCAACCA GTTGCGCTGC CTGCTGCAAC GTGGCGCCTC GGTGACCTTG 
CTCCCCTGGA GCGCTCGTCT CGAGGATGAG CAGTTCGACG CCCTCTTTCT GTCTAACGGA 
CCTGGTAATC CGGAGAGCTG CGACCAGATC GTCCAACAGG TGAGAAAAGT GATTGAGGAG 
GGTCAAAAGC CCGTCTTTGG AATATGTCTG GGTCACCAGC TGTTAGCGAA GGCAATCGGC 
TGCTCTACTT ACAAAATGAA GTACGGCAAT CGAGGACACA ATCTGCCCTG CCTGCACCGA 
GCAACTGGAC GTTGCCTGAT GACGTCGCAG AATCACGGAT ACGCGGTCGA TTTGGAGCAG 
TTGCCCGACG GCTGGTCGGA GCTATTTGTG AATGCCAACG ACGGCACCAA CGAGGGCATT 
GTCCATGCCA GCAAGCCCTA CTTTTCGGTT CAGTTCCATC CGGAGCATCA TGCTGGACCG 
CAGGACACGG AGTTCCTGTT CGATGTCTTT ATGGAGAGCA TTCAACAGAA GGATTTGACC 
ATTCCGCAGC TGATCGAGCA GCGACTGCGT CCGACTACGC CTGCCATAGA TTCGGCTCCA 
GTGATGCCGC GCAAAGTCCT AATCCTGGGA TCCGGTGGAC TGTCCATTGG TCAGGCTGGC 
GAGTTTGACT ACTCCGGATC GCAGGCCATC AAGGCCATGA GGGAATCAAA CATTCAAACA 
GTGCTCATCA ATCCGAACAT AGCCACCGTT CAGACCTCTA AGGGAATGGC CGACAAGTGC 
TACTTCCTTC CCCTAACACC TCACTATGTT GAGCAGGTGA TTAAGTCGGA GCGTCCGAAC 
GGAGTACTGC TCACCTTTGG CGGCCAGACC GCTCTCAATT GTGGCGTGCA ACTGGAGCGA 
GCCGGAGTTT TCTCCAAATA CAATGTCCGC ATTTTGGGCA CACCCATCCA GTCGATCATC 
GAGACGGAGG ATCGCAAGCT CTTTGCCGAG CGGGTCAACG AGATTGGCGA GCAGGTGGCG 
CCATCTGAGG CTGTGTACTC CGTTGCCCAG GCACTGGATG CGGCCAGTCG CTTGGGTTAT 
CCGGTGATGG CCCGTGCTGC CTTCTCCCTG GGCGGACTGG GTTCCGGGTT CGCCAACAAC 
GAGGAGGAGC TGCAGAGCCT GGCCCAACAG GCACTGGCCC ACTCGAGCCA ACTAATTGTG 
GACAAATCCC TGAAGGGTTG GAAGGAGGTG GAGTACGAGG TGGTGCGCGA TGCCTACAAC 
AACTGCATTA CCGTATGCAA CATGGAGAAC TTCGATCCCC TGGGCATCCA CACGGGCGAG 
AGTATTGTGG TGGCCCCATC GCAGACCCTC TCGGATCGCG AGTACCAAAT GCTCCGCAGC 
ACCGCCCTGA AGGTTATCCG TCACTTTGGT GTCGTTGGCG AGTGCAACAT TCAGTACGCC 
TTGTGTCCCC ATTCGGAGCA GTACTACATT ATCGAGGTGA ACGCCCGACT GTCGCGCAGC 
TCCGCGTTGG CCAGCAAGGC CACCGGCTAT CCACTGGCCT ATGTGGCCGC CAAGTTGGCT 
TTGGGATTGC CCCTGCCAGA TATCAAGAAC TCGGTGACGG GCAATACGAC GGCCTGCTTT 
GAGCCATCCC TCGATTACTG TGTGGTCAAG ATTCCACGCT GGGATTTGGC CAAATTCGTG 
CGGGTTAGCA AGCACATTGG CAGCTCGATG AAAAGTGTCG GTGAGGTGAT GGCCATCGGT 
CGCAACTTCG AGGAAGCGTT CCAAAAAGCC CTGCGCATGG TGGACAGCGA TGTGCTTGGC 
TTTGATCCGG ATGTGGTTCC GCTTAACAAG GAGCAGCTGG CCGAGCAGCT TTCGGAGCCG 
ACGGATCGTC GTCCCTTCGT CATTGCCGCT GCTTTGCAAT TGGGCATGAG CCTTAGGGAG 
CTGCATCAAC TAACCAATAT CGATTATTGG TTTCTGGAAA AGCTGGAAAG GATCATCTTA 
CTGCAGTCCC TGCTCACCCG TAATGGCAGT CGAACCGATG CCGCTCTGCT GTTGAAGGCC 
AAGCGTTTTG GTTTTTCGGA CAAGCAGATA GCTAAGTACA TTAAGAGCAC GGAGCTGGCT 
GTTCGTCACC AGCGGCAGGA GTTTGGCATC CGCCCGCATG TCAAGCAGAT CGACACGGTG 
GCCGGCGAAT GGCCCGCCAG TACCAACTAT CTGTACCACA CATATAACGG AAGCGAGCAC 
GATGTGGACT TCCCCGGCGG ACACACTATT GTGGTGGGCT CGGGCGTCTA TCGTATCGGA 
TCCTCAGTGG AGTTTGATTG GTGTGCCGTG GGCTGCCTGC GCGAATTGAG GAAGCTACAG 
CGACCCACCA TCATGATTAA CTACAATCCT GAAACGGTTT CCACCGACTA CGATATGTGC 
GATCGCTTGT ATTTCGAGGA GATTAGCTTC GAGGTGGTCA TGGACATCTA CGAGATGGAG 
AATAGCGAGG GCATCATTCT GTCCATGGGC GGCCAGCTGC CCAACAATAT AGCCATGGAT 
CTGCATCGCC AGCAGGCGAA GGTGTTGGGC ACATCGCCGG AGTCCATCGA CTGTGCCGAA 
AATCGATTTA AATTCTCGCG CATGCTGGAC AGGAAGGGTA TCTTGCAGCC ACGCTGGAAG 
GAGCTGACCA ACCTACAGTC GGCCATCGAA TTTTGCGAGG AGGTTGGCTA TCCCTGTCTG 
GTTAGGCCAT CCTATGTGCT CTCCGGTGCG GCCATGAATG TGGCCTATTC CAATCAGGAT 
CTGGAGACAT ATCTGAACGC CGCCTCCGAG GTGAGTCGCG AGCATCCGGT TGTCATTTCG 
AAGTTCCTCA CCGAGGCCAA GGAGATCGAT GTGGATGCAG TGGCATCCGA TGGACGCATC 
CTGTGCATGG CTGTTTCGGA GCACGTGGAG AATGCCGGAG TGCATTCAGG TGATGCCACG 
CTGGTTACCC CACCGCAGGA TCTGAATGCG GAGACCCTGG AGGCCATTAA ACGGATCACA 
TGCGATTTGG CCAGCGTACT GGATGTCACC GGTCCCTTCA ACATGCAGCT AATTGCCAAG 
AACAACGAGC TGAAGGTCAT CGAATGCAAT GTCCGTGTGT CGCGATCCTT CCCCTTCGTC 
TCCAAGACGC TGGACCACGA CTTTGTGGCC ACGGCGACGC GTGCGATTGT GGGACTCGAT 
GTGGAGCCAC TGGATGTGCT CCATGGCGTC GGCAAGGTGG GCGTCAAGGT GCCACAGTTC 
AGTTTCTCGC GGCTCGCGGG CGCGGATGTA CAGCTGGGCG TCGAGATGGC CTCCACCGGT 
GAGGTTGCCT GCTTCGGGGA CAACCGCTAT GAGGCGTATC TCAAGGCCAT GATGTCCACG 
GGCTTCCAGA TACCCAAGAA CGCCGTACTT CTCTCCATTG GCAGTTTCAA GCACAAGATG 
GAGCTGCTGC CTTCCATACG GGATCTGGCC AAGATGGGTT ACAAGCTATA CGCTTCCATG 
GGCACTGGCG ACTTTTATGC CGAACACGGA GTTAATGTGG AATCCGTGCA GTGGACATTC 
GACAAGACGA CTCCCGATGA CATCAACGGT GAGCTGAGAC ACCTGGCCGA GTTCCTGGCC 
AACAAGCAGT TCGACCTGGT CATCAACCTC CCGATGAGCG GCGGGGGTGC CAGGCGGGTC 
TCCTCGTTCA TGACCCATGG CTACCGCACC CGTCGCCTGG CCGTGGACTA CTCCATTCCG 
CTGGTGACCG ATGTGAAGTG CACCAAGCTT CTGGTAGAAT CAATGCGAAT GAACGGTGGC 
AAGCCGCCAA TGAAGACGCA CACGGATTGT ATGACCTCGC GTAGAATTGT GAAGCTGCCG 
GGCTTCATCG ATGTGCATGT GCATTTGCGG GAGCCGGGAG CCACGCACAA GGAGGACTTT 
GCCAGTGGAA CAGCAGCCGC TCTGGCTGGC GGCGTGACCC TCGTGTGCGC CATGCCCAAT 
ACAAATCCCT CGATCGTGGA CAGAGAGACG TTCACCCAGT TCCAGGAGCT GGCCAAAGCG 
GGAGCTCGAT GCGACTATGC CCTGTATGTG GGGGCCTCGG ATGACAATTG GGCGCAGGTC 
AACGAGCTGG CCAGTCATGC GTGTGGCCTT AAGATGTACT TAAACGACAC TTTCGGCACC 
CTAAAACTGA GCGACATGAC CAGCTGGCAG AGGCATCTCA GCCACTGGCC CAAGCGGTCT 
CCCATCGTTT GTCATGCGGA GAGGCAGAGC ACAGCGGCTG TGATTATGCT GGCCCATCTT 
CTCGACCGTT CGGTTCACAT CTGCCATGTG GCGCGAAAGG AGGAGATCCA ATTGATTCGT 
TCCGCCAAGG AAAAGGGCGT CAAGGTGACC TGCGAGGTGT GCCCCCATCA CTTGTTCCTC 
AGCACCAAGG ATGTGGAGCG TTTGGGTCAC GGAATGTCCG AGGTAAGGCC ATTGCTCTGC 
TCACCGGAGG ATCAAGAGGC TCTGTGGGAG AATATTGATT ACATCGATGT ATTTGCCACC 
GATCACGCGC CACACACACT GGCCGAGAAA CGTTCGGAGC GTCCACCGCC TGGATTTCCG 
GGCGTGGAGA CCATTCTGCC GCTCCTCCTG CAGGCTGTTC ACGAGGGTCG TTTGACGATG 
GAGGACATCA AGAGGAAGTT CCATCGCAAT CCAAAAATCA TCTTCAACCT GCCGGATCAG 
GCGCAGACCT ACGTGGAGGT GGATCTCGAC GAGGAGTGGA CCATAACGGG CAATGAGATG 
AAGAGCAAGT CCGGCTGGAC ACCATTTGAG GGCACCAAGG TCAAGGGACG CGTCCACCGG 
GTGGTTCTTC GCGGCGAGGT GGCCTTCGTC GATGGCCAAG TGTTGGTGCA GCCCGGCTTC 
GGGCAGAATG TGCGCCCCAA ACAGAGTCCA TTGGCGTCGG AGGCATCACA GGATCTGCTG 
CCCAGCGACA ACGATGCCAA TGACACCTTT ACCCGCCTCC TCACCTCCGA AGGACCCGGT 
GGAGGCGTAC ATGGAATATC GACCAAGGTG CACTTTGTGG ACGGAGCCAA CTTCCTGCGC 
CCCAACTCGC CATCCCCACG CATCCGTCTG GATTCCGCCA GCAACACCAC GCTGCGGGAA 
TACTTGCAGC GCACTACCAA CTCAAATCCG GTGGCCCACT CGCTAATGGG CAAGCACATC 
CTTGCCGTTG ACATGTTCAA CAAAGATCAC CTCAACGACA TCTTTAACCT GGCACAGCTG 
CTCAAGCTGC GGGGTACGAA GGATCGTCCG GTGGACGAGC TGCTGCCAGG CAAGATCATG 
GCCAGTGTGT TTTACGAGGT CAGCACGCGG ACGCAGTGCA GCTTTGCAGC TGCCATGCTG 
CGTTTGGGAG GGCGAGTGAT CAGCATGGAC AACATCACGT CGTCGGTGAA GAAGGGCGAG 
AGCCTGGAGG ACAGCATCAA GGTTGTGTCC AGCTATGCCG ACGTCGTGGT GCTCCGTCAT 
CCATCGCCCG GAGCTGTAGC GCGCGCAGCG ACCTTCTCCC GAAAGCCGCT TATCAATGCC 
GGCGATGGCG TCGGTGAGCA TCCCACGCAG GCGCTGCTGG ACATCTTCAC CATCCGCGAG 
GAGTTTGGCA CTGTGAACGG GCTGACCATC ACCATGGTGG GCGACTTGAA GAACGGACGG 
ACCGTGCACT CGCTGGCCCG CCTGCTGACC CTGTACAATG TGAACCTGCA GTATGTGGCG 
CCGAATAGCC TGCAGATGCC CGATGAGGTC GTCCAGTTCG TCCATCAGCG CGGCGTCAAG 
CAGTTATTTG CACGCGACCT AAAGAATGTG CTGCCCGACA CGGATGTGCT CTACATGACT 
CGCATTCAGC GCGAGCGTTT TGATAACGTG GAAGATTACG AGAAGTGCTG TGGCCATCTG 
GTGTTGACGC CCGAGCATAT GATGCGGGCC AAGAAGCGTT CGATTGTTCT GCATCCGCTG 
CCGCGTCTGA ACGAGATCAG CCGGGAGATC GACTCGGACC CAAGGGCCGC CTACTTCCGG 
CAGGCGGAGT ACGGCATGTA CATCCGTATG GCCTTGCTGG CCATGGTCGT TGGTGGACGC 
AATACGGCGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523377.1
CDS	265..6939
Translation	MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQILVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDWLKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSVKERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNGPGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHRATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGPQDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAGEFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPNGVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVAPSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIVDKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRSTALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIGRNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRELHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELAVRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIGSSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEMENSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWKELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVISKFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRITCDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLDVEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMSTGFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVESVQWTFDKTTPDDINGELRHLAEFLANKQFDLVINLPMSGGGARRVSSFMTHGYRTRRLAVDYSIPLVTDVKCTKLLVESMRMNGGKPPMKTHTDCMTSRRIVKLPGFIDVHVHLREPGATHKEDFASGTAAALAGGVTLVCAMPNTNPSIVDRETFTQFQELAKAGARCDYALYVGASDDNWAQVNELASHACGLKMYLNDTFGTLKLSDMTSWQRHLSHWPKRSPIVCHAERQSTAAVIMLAHLLDRSVHICHVARKEEIQLIRSAKEKGVKVTCEVCPHHLFLSTKDVERLGHGMSEVRPLLCSPEDQEALWENIDYIDVFATDHAPHTLAEKRSERPPPGFPGVETILPLLLQAVHEGRLTMEDIKRKFHRNPKIIFNLPDQAQTYVEVDLDEEWTITGNEMKSKSGWTPFEGTKVKGRVHRVVLRGEVAFVDGQVLVQPGFGQNVRPKQSPLASEASQDLLPSDNDANDTFTRLLTSEGPGGGVHGISTKVHFVDGANFLRPNSPSPRIRLDSASNTTLREYLQRTTNSNPVAHSLMGKHILAVDMFNKDHLNDIFNLAQLLKLRGTKDRPVDELLPGKIMASVFYEVSTRTQCSFAAAMLRLGGRVISMDNITSSVKKGESLEDSIKVVSSYADVVVLRHPSPGAVARAATFSRKPLINAGDGVGEHPTQALLDIFTIREEFGTVNGLTITMVGDLKNGRTVHSLARLLTLYNVNLQYVAPNSLQMPDEVVQFVHQRGVKQLFARDLKNVLPDTDVLYMTRIQRERFDNVEDYEKCCGHLVLTPEHMMRAKKRSIVLHPLPRLNEISREIDSDPRAAYFRQAEYGMYIRMALLAMVVGGRNTAL

Target ORF information:

RefSeq Version	NM_078653.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rudimentary (r), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078653.2

ORF Insert Sequence:

ATGGCCTCTA CGGACTGTTA CCTCGCACTG GAGGATGGCA CAGTGTTGCC CGGGTACTCC 
TTTGGCTACG TTCCCTCGGA GAATGAATCC AAAGTGGGTT TTGGTGGCGA GGTGGTCTTC 
CAAACGGGCA TGGTTGGCTA CACGGAGGCG CTCACGGATC GATCGTACAG TGGTCAGATC 
CTGGTGCTTA CCTATCCACT GATCGGTAAC TATGGGGTAC CAGCGCCGGA TGAGGACGAG 
CACGGACTGC CGCTGCACTT CGAGTGGATG AAAGGCGTCG TCCAGGCCAC CGCCTTGGTG 
GTGGGCGAGG TGGCTGAAGA GGCATTTCAC TGGCGCAAAT GGAAGACGCT GCCCGATTGG 
CTGAAGCAGC ACAAGGTGCC CGGAATTCAA GACATTGATA CTCGAGCCCT GACCAAAAAA 
CTGAGGGAGC AGGGAAGCAT GCTAGGCAAG ATTGTCTACG AGAAACCGCC GGTCGAAGGG 
CTGCCCAAGT CCAGTTTCGT AGATCCGAAC GTCAGGAATT TGGCCAAGGA GTGCAGCGTT 
AAGGAGCGAC AAGTGTACGG TAATCCCAAT GGCAAGGGAC CACGGATCGC CATTCTGGAC 
TGTGGACTGA AGCTCAACCA GTTGCGCTGC CTGCTGCAAC GTGGCGCCTC GGTGACCTTG 
CTCCCCTGGA GCGCTCGTCT CGAGGATGAG CAGTTCGACG CCCTCTTTCT GTCTAACGGA 
CCTGGTAATC CGGAGAGCTG CGACCAGATC GTCCAACAGG TGAGAAAAGT GATTGAGGAG 
GGTCAAAAGC CCGTCTTTGG AATATGTCTG GGTCACCAGC TGTTAGCGAA GGCAATCGGC 
TGCTCTACTT ACAAAATGAA GTACGGCAAT CGAGGACACA ATCTGCCCTG CCTGCACCGA 
GCAACTGGAC GTTGCCTGAT GACGTCGCAG AATCACGGAT ACGCGGTCGA TTTGGAGCAG 
TTGCCCGACG GCTGGTCGGA GCTATTTGTG AATGCCAACG ACGGCACCAA CGAGGGCATT 
GTCCATGCCA GCAAGCCCTA CTTTTCGGTT CAGTTCCATC CGGAGCATCA TGCTGGACCG 
CAGGACACGG AGTTCCTGTT CGATGTCTTT ATGGAGAGCA TTCAACAGAA GGATTTGACC 
ATTCCGCAGC TGATCGAGCA GCGACTGCGT CCGACTACGC CTGCCATAGA TTCGGCTCCA 
GTGATGCCGC GCAAAGTCCT AATCCTGGGA TCCGGTGGAC TGTCCATTGG TCAGGCTGGC 
GAGTTTGACT ACTCCGGATC GCAGGCCATC AAGGCCATGA GGGAATCAAA CATTCAAACA 
GTGCTCATCA ATCCGAACAT AGCCACCGTT CAGACCTCTA AGGGAATGGC CGACAAGTGC 
TACTTCCTTC CCCTAACACC TCACTATGTT GAGCAGGTGA TTAAGTCGGA GCGTCCGAAC 
GGAGTACTGC TCACCTTTGG CGGCCAGACC GCTCTCAATT GTGGCGTGCA ACTGGAGCGA 
GCCGGAGTTT TCTCCAAATA CAATGTCCGC ATTTTGGGCA CACCCATCCA GTCGATCATC 
GAGACGGAGG ATCGCAAGCT CTTTGCCGAG CGGGTCAACG AGATTGGCGA GCAGGTGGCG 
CCATCTGAGG CTGTGTACTC CGTTGCCCAG GCACTGGATG CGGCCAGTCG CTTGGGTTAT 
CCGGTGATGG CCCGTGCTGC CTTCTCCCTG GGCGGACTGG GTTCCGGGTT CGCCAACAAC 
GAGGAGGAGC TGCAGAGCCT GGCCCAACAG GCACTGGCCC ACTCGAGCCA ACTAATTGTG 
GACAAATCCC TGAAGGGTTG GAAGGAGGTG GAGTACGAGG TGGTGCGCGA TGCCTACAAC 
AACTGCATTA CCGTATGCAA CATGGAGAAC TTCGATCCCC TGGGCATCCA CACGGGCGAG 
AGTATTGTGG TGGCCCCATC GCAGACCCTC TCGGATCGCG AGTACCAAAT GCTCCGCAGC 
ACCGCCCTGA AGGTTATCCG TCACTTTGGT GTCGTTGGCG AGTGCAACAT TCAGTACGCC 
TTGTGTCCCC ATTCGGAGCA GTACTACATT ATCGAGGTGA ACGCCCGACT GTCGCGCAGC 
TCCGCGTTGG CCAGCAAGGC CACCGGCTAT CCACTGGCCT ATGTGGCCGC CAAGTTGGCT 
TTGGGATTGC CCCTGCCAGA TATCAAGAAC TCGGTGACGG GCAATACGAC GGCCTGCTTT 
GAGCCATCCC TCGATTACTG TGTGGTCAAG ATTCCACGCT GGGATTTGGC CAAATTCGTG 
CGGGTTAGCA AGCACATTGG CAGCTCGATG AAAAGTGTCG GTGAGGTGAT GGCCATCGGT 
CGCAACTTCG AGGAAGCGTT CCAAAAAGCC CTGCGCATGG TGGACAGCGA TGTGCTTGGC 
TTTGATCCGG ATGTGGTTCC GCTTAACAAG GAGCAGCTGG CCGAGCAGCT TTCGGAGCCG 
ACGGATCGTC GTCCCTTCGT CATTGCCGCT GCTTTGCAAT TGGGCATGAG CCTTAGGGAG 
CTGCATCAAC TAACCAATAT CGATTATTGG TTTCTGGAAA AGCTGGAAAG GATCATCTTA 
CTGCAGTCCC TGCTCACCCG TAATGGCAGT CGAACCGATG CCGCTCTGCT GTTGAAGGCC 
AAGCGTTTTG GTTTTTCGGA CAAGCAGATA GCTAAGTACA TTAAGAGCAC GGAGCTGGCT 
GTTCGTCACC AGCGGCAGGA GTTTGGCATC CGCCCGCATG TCAAGCAGAT CGACACGGTG 
GCCGGCGAAT GGCCCGCCAG TACCAACTAT CTGTACCACA CATATAACGG AAGCGAGCAC 
GATGTGGACT TCCCCGGCGG ACACACTATT GTGGTGGGCT CGGGCGTCTA TCGTATCGGA 
TCCTCAGTGG AGTTTGATTG GTGTGCCGTG GGCTGCCTGC GCGAATTGAG GAAGCTACAG 
CGACCCACCA TCATGATTAA CTACAATCCT GAAACGGTTT CCACCGACTA CGATATGTGC 
GATCGCTTGT ATTTCGAGGA GATTAGCTTC GAGGTGGTCA TGGACATCTA CGAGATGGAG 
AATAGCGAGG GCATCATTCT GTCCATGGGC GGCCAGCTGC CCAACAATAT AGCCATGGAT 
CTGCATCGCC AGCAGGCGAA GGTGTTGGGC ACATCGCCGG AGTCCATCGA CTGTGCCGAA 
AATCGATTTA AATTCTCGCG CATGCTGGAC AGGAAGGGTA TCTTGCAGCC ACGCTGGAAG 
GAGCTGACCA ACCTACAGTC GGCCATCGAA TTTTGCGAGG AGGTTGGCTA TCCCTGTCTG 
GTTAGGCCAT CCTATGTGCT CTCCGGTGCG GCCATGAATG TGGCCTATTC CAATCAGGAT 
CTGGAGACAT ATCTGAACGC CGCCTCCGAG GTGAGTCGCG AGCATCCGGT TGTCATTTCG 
AAGTTCCTCA CCGAGGCCAA GGAGATCGAT GTGGATGCAG TGGCATCCGA TGGACGCATC 
CTGTGCATGG CTGTTTCGGA GCACGTGGAG AATGCCGGAG TGCATTCAGG TGATGCCACG 
CTGGTTACCC CACCGCAGGA TCTGAATGCG GAGACCCTGG AGGCCATTAA ACGGATCACA 
TGCGATTTGG CCAGCGTACT GGATGTCACC GGTCCCTTCA ACATGCAGCT AATTGCCAAG 
AACAACGAGC TGAAGGTCAT CGAATGCAAT GTCCGTGTGT CGCGATCCTT CCCCTTCGTC 
TCCAAGACGC TGGACCACGA CTTTGTGGCC ACGGCGACGC GTGCGATTGT GGGACTCGAT 
GTGGAGCCAC TGGATGTGCT CCATGGCGTC GGCAAGGTGG GCGTCAAGGT GCCACAGTTC 
AGTTTCTCGC GGCTCGCGGG CGCGGATGTA CAGCTGGGCG TCGAGATGGC CTCCACCGGT 
GAGGTTGCCT GCTTCGGGGA CAACCGCTAT GAGGCGTATC TCAAGGCCAT GATGTCCACG 
GGCTTCCAGA TACCCAAGAA CGCCGTACTT CTCTCCATTG GCAGTTTCAA GCACAAGATG 
GAGCTGCTGC CTTCCATACG GGATCTGGCC AAGATGGGTT ACAAGCTATA CGCTTCCATG 
GGCACTGGCG ACTTTTATGC CGAACACGGA GTTAATGTGG AATCCGTGCA GTGGACATTC 
GACAAGACGA CTCCCGATGA CATCAACGGT GAGCTGAGAC ACCTGGCCGA GTTCCTGGCC 
AACAAGCAGT TCGACCTGGT CATCAACCTC CCGATGAGCG GCGGGGGTGC CAGGCGGGTC 
TCCTCGTTCA TGACCCATGG CTACCGCACC CGTCGCCTGG CCGTGGACTA CTCCATTCCG 
CTGGTGACCG ATGTGAAGTG CACCAAGCTT CTGGTAGAAT CAATGCGAAT GAACGGTGGC 
AAGCCGCCAA TGAAGACGCA CACGGATTGT ATGACCTCGC GTAGAATTGT GAAGCTGCCG 
GGCTTCATCG ATGTGCATGT GCATTTGCGG GAGCCGGGAG CCACGCACAA GGAGGACTTT 
GCCAGTGGAA CAGCAGCCGC TCTGGCTGGC GGCGTGACCC TCGTGTGCGC CATGCCCAAT 
ACAAATCCCT CGATCGTGGA CAGAGAGACG TTCACCCAGT TCCAGGAGCT GGCCAAAGCG 
GGAGCTCGAT GCGACTATGC CCTGTATGTG GGGGCCTCGG ATGACAATTG GGCGCAGGTC 
AACGAGCTGG CCAGTCATGC GTGTGGCCTT AAGATGTACT TAAACGACAC TTTCGGCACC 
CTAAAACTGA GCGACATGAC CAGCTGGCAG AGGCATCTCA GCCACTGGCC CAAGCGGTCT 
CCCATCGTTT GTCATGCGGA GAGGCAGAGC ACAGCGGCTG TGATTATGCT GGCCCATCTT 
CTCGACCGTT CGGTTCACAT CTGCCATGTG GCGCGAAAGG AGGAGATCCA ATTGATTCGT 
TCCGCCAAGG AAAAGGGCGT CAAGGTGACC TGCGAGGTGT GCCCCCATCA CTTGTTCCTC 
AGCACCAAGG ATGTGGAGCG TTTGGGTCAC GGAATGTCCG AGGTAAGGCC ATTGCTCTGC 
TCACCGGAGG ATCAAGAGGC TCTGTGGGAG AATATTGATT ACATCGATGT ATTTGCCACC 
GATCACGCGC CACACACACT GGCCGAGAAA CGTTCGGAGC GTCCACCGCC TGGATTTCCG 
GGCGTGGAGA CCATTCTGCC GCTCCTCCTG CAGGCTGTTC ACGAGGGTCG TTTGACGATG 
GAGGACATCA AGAGGAAGTT CCATCGCAAT CCAAAAATCA TCTTCAACCT GCCGGATCAG 
GCGCAGACCT ACGTGGAGGT GGATCTCGAC GAGGAGTGGA CCATAACGGG CAATGAGATG 
AAGAGCAAGT CCGGCTGGAC ACCATTTGAG GGCACCAAGG TCAAGGGACG CGTCCACCGG 
GTGGTTCTTC GCGGCGAGGT GGCCTTCGTC GATGGCCAAG TGTTGGTGCA GCCCGGCTTC 
GGGCAGAATG TGCGCCCCAA ACAGAGTCCA TTGGCGTCGG AGGCATCACA GGATCTGCTG 
CCCAGCGACA ACGATGCCAA TGACACCTTT ACCCGCCTCC TCACCTCCGA AGGACCCGGT 
GGAGGCGTAC ATGGAATATC GACCAAGGTG CACTTTGTGG ACGGAGCCAA CTTCCTGCGC 
CCCAACTCGC CATCCCCACG CATCCGTCTG GATTCCGCCA GCAACACCAC GCTGCGGGAA 
TACTTGCAGC GCACTACCAA CTCAAATCCG GTGGCCCACT CGCTAATGGG CAAGCACATC 
CTTGCCGTTG ACATGTTCAA CAAAGATCAC CTCAACGACA TCTTTAACCT GGCACAGCTG 
CTCAAGCTGC GGGGTACGAA GGATCGTCCG GTGGACGAGC TGCTGCCAGG CAAGATCATG 
GCCAGTGTGT TTTACGAGGT CAGCACGCGG ACGCAGTGCA GCTTTGCAGC TGCCATGCTG 
CGTTTGGGAG GGCGAGTGAT CAGCATGGAC AACATCACGT CGTCGGTGAA GAAGGGCGAG 
AGCCTGGAGG ACAGCATCAA GGTTGTGTCC AGCTATGCCG ACGTCGTGGT GCTCCGTCAT 
CCATCGCCCG GAGCTGTAGC GCGCGCAGCG ACCTTCTCCC GAAAGCCGCT TATCAATGCC 
GGCGATGGCG TCGGTGAGCA TCCCACGCAG GCGCTGCTGG ACATCTTCAC CATCCGCGAG 
GAGTTTGGCA CTGTGAACGG GCTGACCATC ACCATGGTGG GCGACTTGAA GAACGGACGG 
ACCGTGCACT CGCTGGCCCG CCTGCTGACC CTGTACAATG TGAACCTGCA GTATGTGGCG 
CCGAATAGCC TGCAGATGCC CGATGAGGTC GTCCAGTTCG TCCATCAGCG CGGCGTCAAG 
CAGTTATTTG CACGCGACCT AAAGAATGTG CTGCCCGACA CGGATGTGCT CTACATGACT 
CGCATTCAGC GCGAGCGTTT TGATAACGTG GAAGATTACG AGAAGTGCTG TGGCCATCTG 
GTGTTGACGC CCGAGCATAT GATGCGGGCC AAGAAGCGTT CGATTGTTCT GCATCCGCTG 
CCGCGTCTGA ACGAGATCAG CCGGGAGATC GACTCGGACC CAAGGGCCGC CTACTTCCGG 
CAGGCGGAGT ACGGCATGTA CATCCGTATG GCCTTGCTGG CCATGGTCGT TGGTGGACGC 
AATACGGCGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

r ( NM_206765.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996488.2 Drosophila melanogaster rudimentary (r), transcript variant E, mRNA.

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CloneID	OFa03045
Clone ID Related Accession (Same CDS sequence)	NM_001298414.1 , NM_078653.2 , NM_206765.3
Accession Version	NM_206765.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rudimentary, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Oct 19, 2015 this sequence version replaced NM_206765.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCTCTA CGGACTGTTA CCTCGCACTG GAGGATGGCA CAGTGTTGCC CGGGTACTCC 
TTTGGCTACG TTCCCTCGGA GAATGAATCC AAAGTGGGTT TTGGTGGCGA GGTGGTCTTC 
CAAACGGGCA TGGTTGGCTA CACGGAGGCG CTCACGGATC GATCGTACAG TGGTCAGATC 
CTGGTGCTTA CCTATCCACT GATCGGTAAC TATGGGGTAC CAGCGCCGGA TGAGGACGAG 
CACGGACTGC CGCTGCACTT CGAGTGGATG AAAGGCGTCG TCCAGGCCAC CGCCTTGGTG 
GTGGGCGAGG TGGCTGAAGA GGCATTTCAC TGGCGCAAAT GGAAGACGCT GCCCGATTGG 
CTGAAGCAGC ACAAGGTGCC CGGAATTCAA GACATTGATA CTCGAGCCCT GACCAAAAAA 
CTGAGGGAGC AGGGAAGCAT GCTAGGCAAG ATTGTCTACG AGAAACCGCC GGTCGAAGGG 
CTGCCCAAGT CCAGTTTCGT AGATCCGAAC GTCAGGAATT TGGCCAAGGA GTGCAGCGTT 
AAGGAGCGAC AAGTGTACGG TAATCCCAAT GGCAAGGGAC CACGGATCGC CATTCTGGAC 
TGTGGACTGA AGCTCAACCA GTTGCGCTGC CTGCTGCAAC GTGGCGCCTC GGTGACCTTG 
CTCCCCTGGA GCGCTCGTCT CGAGGATGAG CAGTTCGACG CCCTCTTTCT GTCTAACGGA 
CCTGGTAATC CGGAGAGCTG CGACCAGATC GTCCAACAGG TGAGAAAAGT GATTGAGGAG 
GGTCAAAAGC CCGTCTTTGG AATATGTCTG GGTCACCAGC TGTTAGCGAA GGCAATCGGC 
TGCTCTACTT ACAAAATGAA GTACGGCAAT CGAGGACACA ATCTGCCCTG CCTGCACCGA 
GCAACTGGAC GTTGCCTGAT GACGTCGCAG AATCACGGAT ACGCGGTCGA TTTGGAGCAG 
TTGCCCGACG GCTGGTCGGA GCTATTTGTG AATGCCAACG ACGGCACCAA CGAGGGCATT 
GTCCATGCCA GCAAGCCCTA CTTTTCGGTT CAGTTCCATC CGGAGCATCA TGCTGGACCG 
CAGGACACGG AGTTCCTGTT CGATGTCTTT ATGGAGAGCA TTCAACAGAA GGATTTGACC 
ATTCCGCAGC TGATCGAGCA GCGACTGCGT CCGACTACGC CTGCCATAGA TTCGGCTCCA 
GTGATGCCGC GCAAAGTCCT AATCCTGGGA TCCGGTGGAC TGTCCATTGG TCAGGCTGGC 
GAGTTTGACT ACTCCGGATC GCAGGCCATC AAGGCCATGA GGGAATCAAA CATTCAAACA 
GTGCTCATCA ATCCGAACAT AGCCACCGTT CAGACCTCTA AGGGAATGGC CGACAAGTGC 
TACTTCCTTC CCCTAACACC TCACTATGTT GAGCAGGTGA TTAAGTCGGA GCGTCCGAAC 
GGAGTACTGC TCACCTTTGG CGGCCAGACC GCTCTCAATT GTGGCGTGCA ACTGGAGCGA 
GCCGGAGTTT TCTCCAAATA CAATGTCCGC ATTTTGGGCA CACCCATCCA GTCGATCATC 
GAGACGGAGG ATCGCAAGCT CTTTGCCGAG CGGGTCAACG AGATTGGCGA GCAGGTGGCG 
CCATCTGAGG CTGTGTACTC CGTTGCCCAG GCACTGGATG CGGCCAGTCG CTTGGGTTAT 
CCGGTGATGG CCCGTGCTGC CTTCTCCCTG GGCGGACTGG GTTCCGGGTT CGCCAACAAC 
GAGGAGGAGC TGCAGAGCCT GGCCCAACAG GCACTGGCCC ACTCGAGCCA ACTAATTGTG 
GACAAATCCC TGAAGGGTTG GAAGGAGGTG GAGTACGAGG TGGTGCGCGA TGCCTACAAC 
AACTGCATTA CCGTATGCAA CATGGAGAAC TTCGATCCCC TGGGCATCCA CACGGGCGAG 
AGTATTGTGG TGGCCCCATC GCAGACCCTC TCGGATCGCG AGTACCAAAT GCTCCGCAGC 
ACCGCCCTGA AGGTTATCCG TCACTTTGGT GTCGTTGGCG AGTGCAACAT TCAGTACGCC 
TTGTGTCCCC ATTCGGAGCA GTACTACATT ATCGAGGTGA ACGCCCGACT GTCGCGCAGC 
TCCGCGTTGG CCAGCAAGGC CACCGGCTAT CCACTGGCCT ATGTGGCCGC CAAGTTGGCT 
TTGGGATTGC CCCTGCCAGA TATCAAGAAC TCGGTGACGG GCAATACGAC GGCCTGCTTT 
GAGCCATCCC TCGATTACTG TGTGGTCAAG ATTCCACGCT GGGATTTGGC CAAATTCGTG 
CGGGTTAGCA AGCACATTGG CAGCTCGATG AAAAGTGTCG GTGAGGTGAT GGCCATCGGT 
CGCAACTTCG AGGAAGCGTT CCAAAAAGCC CTGCGCATGG TGGACAGCGA TGTGCTTGGC 
TTTGATCCGG ATGTGGTTCC GCTTAACAAG GAGCAGCTGG CCGAGCAGCT TTCGGAGCCG 
ACGGATCGTC GTCCCTTCGT CATTGCCGCT GCTTTGCAAT TGGGCATGAG CCTTAGGGAG 
CTGCATCAAC TAACCAATAT CGATTATTGG TTTCTGGAAA AGCTGGAAAG GATCATCTTA 
CTGCAGTCCC TGCTCACCCG TAATGGCAGT CGAACCGATG CCGCTCTGCT GTTGAAGGCC 
AAGCGTTTTG GTTTTTCGGA CAAGCAGATA GCTAAGTACA TTAAGAGCAC GGAGCTGGCT 
GTTCGTCACC AGCGGCAGGA GTTTGGCATC CGCCCGCATG TCAAGCAGAT CGACACGGTG 
GCCGGCGAAT GGCCCGCCAG TACCAACTAT CTGTACCACA CATATAACGG AAGCGAGCAC 
GATGTGGACT TCCCCGGCGG ACACACTATT GTGGTGGGCT CGGGCGTCTA TCGTATCGGA 
TCCTCAGTGG AGTTTGATTG GTGTGCCGTG GGCTGCCTGC GCGAATTGAG GAAGCTACAG 
CGACCCACCA TCATGATTAA CTACAATCCT GAAACGGTTT CCACCGACTA CGATATGTGC 
GATCGCTTGT ATTTCGAGGA GATTAGCTTC GAGGTGGTCA TGGACATCTA CGAGATGGAG 
AATAGCGAGG GCATCATTCT GTCCATGGGC GGCCAGCTGC CCAACAATAT AGCCATGGAT 
CTGCATCGCC AGCAGGCGAA GGTGTTGGGC ACATCGCCGG AGTCCATCGA CTGTGCCGAA 
AATCGATTTA AATTCTCGCG CATGCTGGAC AGGAAGGGTA TCTTGCAGCC ACGCTGGAAG 
GAGCTGACCA ACCTACAGTC GGCCATCGAA TTTTGCGAGG AGGTTGGCTA TCCCTGTCTG 
GTTAGGCCAT CCTATGTGCT CTCCGGTGCG GCCATGAATG TGGCCTATTC CAATCAGGAT 
CTGGAGACAT ATCTGAACGC CGCCTCCGAG GTGAGTCGCG AGCATCCGGT TGTCATTTCG 
AAGTTCCTCA CCGAGGCCAA GGAGATCGAT GTGGATGCAG TGGCATCCGA TGGACGCATC 
CTGTGCATGG CTGTTTCGGA GCACGTGGAG AATGCCGGAG TGCATTCAGG TGATGCCACG 
CTGGTTACCC CACCGCAGGA TCTGAATGCG GAGACCCTGG AGGCCATTAA ACGGATCACA 
TGCGATTTGG CCAGCGTACT GGATGTCACC GGTCCCTTCA ACATGCAGCT AATTGCCAAG 
AACAACGAGC TGAAGGTCAT CGAATGCAAT GTCCGTGTGT CGCGATCCTT CCCCTTCGTC 
TCCAAGACGC TGGACCACGA CTTTGTGGCC ACGGCGACGC GTGCGATTGT GGGACTCGAT 
GTGGAGCCAC TGGATGTGCT CCATGGCGTC GGCAAGGTGG GCGTCAAGGT GCCACAGTTC 
AGTTTCTCGC GGCTCGCGGG CGCGGATGTA CAGCTGGGCG TCGAGATGGC CTCCACCGGT 
GAGGTTGCCT GCTTCGGGGA CAACCGCTAT GAGGCGTATC TCAAGGCCAT GATGTCCACG 
GGCTTCCAGA TACCCAAGAA CGCCGTACTT CTCTCCATTG GCAGTTTCAA GCACAAGATG 
GAGCTGCTGC CTTCCATACG GGATCTGGCC AAGATGGGTT ACAAGCTATA CGCTTCCATG 
GGCACTGGCG ACTTTTATGC CGAACACGGA GTTAATGTGG AATCCGTGCA GTGGACATTC 
GACAAGACGA CTCCCGATGA CATCAACGGT GAGCTGAGAC ACCTGGCCGA GTTCCTGGCC 
AACAAGCAGT TCGACCTGGT CATCAACCTC CCGATGAGCG GCGGGGGTGC CAGGCGGGTC 
TCCTCGTTCA TGACCCATGG CTACCGCACC CGTCGCCTGG CCGTGGACTA CTCCATTCCG 
CTGGTGACCG ATGTGAAGTG CACCAAGCTT CTGGTAGAAT CAATGCGAAT GAACGGTGGC 
AAGCCGCCAA TGAAGACGCA CACGGATTGT ATGACCTCGC GTAGAATTGT GAAGCTGCCG 
GGCTTCATCG ATGTGCATGT GCATTTGCGG GAGCCGGGAG CCACGCACAA GGAGGACTTT 
GCCAGTGGAA CAGCAGCCGC TCTGGCTGGC GGCGTGACCC TCGTGTGCGC CATGCCCAAT 
ACAAATCCCT CGATCGTGGA CAGAGAGACG TTCACCCAGT TCCAGGAGCT GGCCAAAGCG 
GGAGCTCGAT GCGACTATGC CCTGTATGTG GGGGCCTCGG ATGACAATTG GGCGCAGGTC 
AACGAGCTGG CCAGTCATGC GTGTGGCCTT AAGATGTACT TAAACGACAC TTTCGGCACC 
CTAAAACTGA GCGACATGAC CAGCTGGCAG AGGCATCTCA GCCACTGGCC CAAGCGGTCT 
CCCATCGTTT GTCATGCGGA GAGGCAGAGC ACAGCGGCTG TGATTATGCT GGCCCATCTT 
CTCGACCGTT CGGTTCACAT CTGCCATGTG GCGCGAAAGG AGGAGATCCA ATTGATTCGT 
TCCGCCAAGG AAAAGGGCGT CAAGGTGACC TGCGAGGTGT GCCCCCATCA CTTGTTCCTC 
AGCACCAAGG ATGTGGAGCG TTTGGGTCAC GGAATGTCCG AGGTAAGGCC ATTGCTCTGC 
TCACCGGAGG ATCAAGAGGC TCTGTGGGAG AATATTGATT ACATCGATGT ATTTGCCACC 
GATCACGCGC CACACACACT GGCCGAGAAA CGTTCGGAGC GTCCACCGCC TGGATTTCCG 
GGCGTGGAGA CCATTCTGCC GCTCCTCCTG CAGGCTGTTC ACGAGGGTCG TTTGACGATG 
GAGGACATCA AGAGGAAGTT CCATCGCAAT CCAAAAATCA TCTTCAACCT GCCGGATCAG 
GCGCAGACCT ACGTGGAGGT GGATCTCGAC GAGGAGTGGA CCATAACGGG CAATGAGATG 
AAGAGCAAGT CCGGCTGGAC ACCATTTGAG GGCACCAAGG TCAAGGGACG CGTCCACCGG 
GTGGTTCTTC GCGGCGAGGT GGCCTTCGTC GATGGCCAAG TGTTGGTGCA GCCCGGCTTC 
GGGCAGAATG TGCGCCCCAA ACAGAGTCCA TTGGCGTCGG AGGCATCACA GGATCTGCTG 
CCCAGCGACA ACGATGCCAA TGACACCTTT ACCCGCCTCC TCACCTCCGA AGGACCCGGT 
GGAGGCGTAC ATGGAATATC GACCAAGGTG CACTTTGTGG ACGGAGCCAA CTTCCTGCGC 
CCCAACTCGC CATCCCCACG CATCCGTCTG GATTCCGCCA GCAACACCAC GCTGCGGGAA 
TACTTGCAGC GCACTACCAA CTCAAATCCG GTGGCCCACT CGCTAATGGG CAAGCACATC 
CTTGCCGTTG ACATGTTCAA CAAAGATCAC CTCAACGACA TCTTTAACCT GGCACAGCTG 
CTCAAGCTGC GGGGTACGAA GGATCGTCCG GTGGACGAGC TGCTGCCAGG CAAGATCATG 
GCCAGTGTGT TTTACGAGGT CAGCACGCGG ACGCAGTGCA GCTTTGCAGC TGCCATGCTG 
CGTTTGGGAG GGCGAGTGAT CAGCATGGAC AACATCACGT CGTCGGTGAA GAAGGGCGAG 
AGCCTGGAGG ACAGCATCAA GGTTGTGTCC AGCTATGCCG ACGTCGTGGT GCTCCGTCAT 
CCATCGCCCG GAGCTGTAGC GCGCGCAGCG ACCTTCTCCC GAAAGCCGCT TATCAATGCC 
GGCGATGGCG TCGGTGAGCA TCCCACGCAG GCGCTGCTGG ACATCTTCAC CATCCGCGAG 
GAGTTTGGCA CTGTGAACGG GCTGACCATC ACCATGGTGG GCGACTTGAA GAACGGACGG 
ACCGTGCACT CGCTGGCCCG CCTGCTGACC CTGTACAATG TGAACCTGCA GTATGTGGCG 
CCGAATAGCC TGCAGATGCC CGATGAGGTC GTCCAGTTCG TCCATCAGCG CGGCGTCAAG 
CAGTTATTTG CACGCGACCT AAAGAATGTG CTGCCCGACA CGGATGTGCT CTACATGACT 
CGCATTCAGC GCGAGCGTTT TGATAACGTG GAAGATTACG AGAAGTGCTG TGGCCATCTG 
GTGTTGACGC CCGAGCATAT GATGCGGGCC AAGAAGCGTT CGATTGTTCT GCATCCGCTG 
CCGCGTCTGA ACGAGATCAG CCGGGAGATC GACTCGGACC CAAGGGCCGC CTACTTCCGG 
CAGGCGGAGT ACGGCATGTA CATCCGTATG GCCTTGCTGG CCATGGTCGT TGGTGGACGC 
AATACGGCGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996488.2
CDS	154..6828
Translation	MASTDCYLALEDGTVLPGYSFGYVPSENESKVGFGGEVVFQTGMVGYTEALTDRSYSGQILVLTYPLIGNYGVPAPDEDEHGLPLHFEWMKGVVQATALVVGEVAEEAFHWRKWKTLPDWLKQHKVPGIQDIDTRALTKKLREQGSMLGKIVYEKPPVEGLPKSSFVDPNVRNLAKECSVKERQVYGNPNGKGPRIAILDCGLKLNQLRCLLQRGASVTLLPWSARLEDEQFDALFLSNGPGNPESCDQIVQQVRKVIEEGQKPVFGICLGHQLLAKAIGCSTYKMKYGNRGHNLPCLHRATGRCLMTSQNHGYAVDLEQLPDGWSELFVNANDGTNEGIVHASKPYFSVQFHPEHHAGPQDTEFLFDVFMESIQQKDLTIPQLIEQRLRPTTPAIDSAPVMPRKVLILGSGGLSIGQAGEFDYSGSQAIKAMRESNIQTVLINPNIATVQTSKGMADKCYFLPLTPHYVEQVIKSERPNGVLLTFGGQTALNCGVQLERAGVFSKYNVRILGTPIQSIIETEDRKLFAERVNEIGEQVAPSEAVYSVAQALDAASRLGYPVMARAAFSLGGLGSGFANNEEELQSLAQQALAHSSQLIVDKSLKGWKEVEYEVVRDAYNNCITVCNMENFDPLGIHTGESIVVAPSQTLSDREYQMLRSTALKVIRHFGVVGECNIQYALCPHSEQYYIIEVNARLSRSSALASKATGYPLAYVAAKLALGLPLPDIKNSVTGNTTACFEPSLDYCVVKIPRWDLAKFVRVSKHIGSSMKSVGEVMAIGRNFEEAFQKALRMVDSDVLGFDPDVVPLNKEQLAEQLSEPTDRRPFVIAAALQLGMSLRELHQLTNIDYWFLEKLERIILLQSLLTRNGSRTDAALLLKAKRFGFSDKQIAKYIKSTELAVRHQRQEFGIRPHVKQIDTVAGEWPASTNYLYHTYNGSEHDVDFPGGHTIVVGSGVYRIGSSVEFDWCAVGCLRELRKLQRPTIMINYNPETVSTDYDMCDRLYFEEISFEVVMDIYEMENSEGIILSMGGQLPNNIAMDLHRQQAKVLGTSPESIDCAENRFKFSRMLDRKGILQPRWKELTNLQSAIEFCEEVGYPCLVRPSYVLSGAAMNVAYSNQDLETYLNAASEVSREHPVVISKFLTEAKEIDVDAVASDGRILCMAVSEHVENAGVHSGDATLVTPPQDLNAETLEAIKRITCDLASVLDVTGPFNMQLIAKNNELKVIECNVRVSRSFPFVSKTLDHDFVATATRAIVGLDVEPLDVLHGVGKVGVKVPQFSFSRLAGADVQLGVEMASTGEVACFGDNRYEAYLKAMMSTGFQIPKNAVLLSIGSFKHKMELLPSIRDLAKMGYKLYASMGTGDFYAEHGVNVESVQWTFDKTTPDDINGELRHLAEFLANKQFDLVINLPMSGGGARRVSSFMTHGYRTRRLAVDYSIPLVTDVKCTKLLVESMRMNGGKPPMKTHTDCMTSRRIVKLPGFIDVHVHLREPGATHKEDFASGTAAALAGGVTLVCAMPNTNPSIVDRETFTQFQELAKAGARCDYALYVGASDDNWAQVNELASHACGLKMYLNDTFGTLKLSDMTSWQRHLSHWPKRSPIVCHAERQSTAAVIMLAHLLDRSVHICHVARKEEIQLIRSAKEKGVKVTCEVCPHHLFLSTKDVERLGHGMSEVRPLLCSPEDQEALWENIDYIDVFATDHAPHTLAEKRSERPPPGFPGVETILPLLLQAVHEGRLTMEDIKRKFHRNPKIIFNLPDQAQTYVEVDLDEEWTITGNEMKSKSGWTPFEGTKVKGRVHRVVLRGEVAFVDGQVLVQPGFGQNVRPKQSPLASEASQDLLPSDNDANDTFTRLLTSEGPGGGVHGISTKVHFVDGANFLRPNSPSPRIRLDSASNTTLREYLQRTTNSNPVAHSLMGKHILAVDMFNKDHLNDIFNLAQLLKLRGTKDRPVDELLPGKIMASVFYEVSTRTQCSFAAAMLRLGGRVISMDNITSSVKKGESLEDSIKVVSSYADVVVLRHPSPGAVARAATFSRKPLINAGDGVGEHPTQALLDIFTIREEFGTVNGLTITMVGDLKNGRTVHSLARLLTLYNVNLQYVAPNSLQMPDEVVQFVHQRGVKQLFARDLKNVLPDTDVLYMTRIQRERFDNVEDYEKCCGHLVLTPEHMMRAKKRSIVLHPLPRLNEISREIDSDPRAAYFRQAEYGMYIRMALLAMVVGGRNTAL

Target ORF information:

RefSeq Version	NM_206765.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rudimentary (r), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206765.3

ORF Insert Sequence:

ATGGCCTCTA CGGACTGTTA CCTCGCACTG GAGGATGGCA CAGTGTTGCC CGGGTACTCC 
TTTGGCTACG TTCCCTCGGA GAATGAATCC AAAGTGGGTT TTGGTGGCGA GGTGGTCTTC 
CAAACGGGCA TGGTTGGCTA CACGGAGGCG CTCACGGATC GATCGTACAG TGGTCAGATC 
CTGGTGCTTA CCTATCCACT GATCGGTAAC TATGGGGTAC CAGCGCCGGA TGAGGACGAG 
CACGGACTGC CGCTGCACTT CGAGTGGATG AAAGGCGTCG TCCAGGCCAC CGCCTTGGTG 
GTGGGCGAGG TGGCTGAAGA GGCATTTCAC TGGCGCAAAT GGAAGACGCT GCCCGATTGG 
CTGAAGCAGC ACAAGGTGCC CGGAATTCAA GACATTGATA CTCGAGCCCT GACCAAAAAA 
CTGAGGGAGC AGGGAAGCAT GCTAGGCAAG ATTGTCTACG AGAAACCGCC GGTCGAAGGG 
CTGCCCAAGT CCAGTTTCGT AGATCCGAAC GTCAGGAATT TGGCCAAGGA GTGCAGCGTT 
AAGGAGCGAC AAGTGTACGG TAATCCCAAT GGCAAGGGAC CACGGATCGC CATTCTGGAC 
TGTGGACTGA AGCTCAACCA GTTGCGCTGC CTGCTGCAAC GTGGCGCCTC GGTGACCTTG 
CTCCCCTGGA GCGCTCGTCT CGAGGATGAG CAGTTCGACG CCCTCTTTCT GTCTAACGGA 
CCTGGTAATC CGGAGAGCTG CGACCAGATC GTCCAACAGG TGAGAAAAGT GATTGAGGAG 
GGTCAAAAGC CCGTCTTTGG AATATGTCTG GGTCACCAGC TGTTAGCGAA GGCAATCGGC 
TGCTCTACTT ACAAAATGAA GTACGGCAAT CGAGGACACA ATCTGCCCTG CCTGCACCGA 
GCAACTGGAC GTTGCCTGAT GACGTCGCAG AATCACGGAT ACGCGGTCGA TTTGGAGCAG 
TTGCCCGACG GCTGGTCGGA GCTATTTGTG AATGCCAACG ACGGCACCAA CGAGGGCATT 
GTCCATGCCA GCAAGCCCTA CTTTTCGGTT CAGTTCCATC CGGAGCATCA TGCTGGACCG 
CAGGACACGG AGTTCCTGTT CGATGTCTTT ATGGAGAGCA TTCAACAGAA GGATTTGACC 
ATTCCGCAGC TGATCGAGCA GCGACTGCGT CCGACTACGC CTGCCATAGA TTCGGCTCCA 
GTGATGCCGC GCAAAGTCCT AATCCTGGGA TCCGGTGGAC TGTCCATTGG TCAGGCTGGC 
GAGTTTGACT ACTCCGGATC GCAGGCCATC AAGGCCATGA GGGAATCAAA CATTCAAACA 
GTGCTCATCA ATCCGAACAT AGCCACCGTT CAGACCTCTA AGGGAATGGC CGACAAGTGC 
TACTTCCTTC CCCTAACACC TCACTATGTT GAGCAGGTGA TTAAGTCGGA GCGTCCGAAC 
GGAGTACTGC TCACCTTTGG CGGCCAGACC GCTCTCAATT GTGGCGTGCA ACTGGAGCGA 
GCCGGAGTTT TCTCCAAATA CAATGTCCGC ATTTTGGGCA CACCCATCCA GTCGATCATC 
GAGACGGAGG ATCGCAAGCT CTTTGCCGAG CGGGTCAACG AGATTGGCGA GCAGGTGGCG 
CCATCTGAGG CTGTGTACTC CGTTGCCCAG GCACTGGATG CGGCCAGTCG CTTGGGTTAT 
CCGGTGATGG CCCGTGCTGC CTTCTCCCTG GGCGGACTGG GTTCCGGGTT CGCCAACAAC 
GAGGAGGAGC TGCAGAGCCT GGCCCAACAG GCACTGGCCC ACTCGAGCCA ACTAATTGTG 
GACAAATCCC TGAAGGGTTG GAAGGAGGTG GAGTACGAGG TGGTGCGCGA TGCCTACAAC 
AACTGCATTA CCGTATGCAA CATGGAGAAC TTCGATCCCC TGGGCATCCA CACGGGCGAG 
AGTATTGTGG TGGCCCCATC GCAGACCCTC TCGGATCGCG AGTACCAAAT GCTCCGCAGC 
ACCGCCCTGA AGGTTATCCG TCACTTTGGT GTCGTTGGCG AGTGCAACAT TCAGTACGCC 
TTGTGTCCCC ATTCGGAGCA GTACTACATT ATCGAGGTGA ACGCCCGACT GTCGCGCAGC 
TCCGCGTTGG CCAGCAAGGC CACCGGCTAT CCACTGGCCT ATGTGGCCGC CAAGTTGGCT 
TTGGGATTGC CCCTGCCAGA TATCAAGAAC TCGGTGACGG GCAATACGAC GGCCTGCTTT 
GAGCCATCCC TCGATTACTG TGTGGTCAAG ATTCCACGCT GGGATTTGGC CAAATTCGTG 
CGGGTTAGCA AGCACATTGG CAGCTCGATG AAAAGTGTCG GTGAGGTGAT GGCCATCGGT 
CGCAACTTCG AGGAAGCGTT CCAAAAAGCC CTGCGCATGG TGGACAGCGA TGTGCTTGGC 
TTTGATCCGG ATGTGGTTCC GCTTAACAAG GAGCAGCTGG CCGAGCAGCT TTCGGAGCCG 
ACGGATCGTC GTCCCTTCGT CATTGCCGCT GCTTTGCAAT TGGGCATGAG CCTTAGGGAG 
CTGCATCAAC TAACCAATAT CGATTATTGG TTTCTGGAAA AGCTGGAAAG GATCATCTTA 
CTGCAGTCCC TGCTCACCCG TAATGGCAGT CGAACCGATG CCGCTCTGCT GTTGAAGGCC 
AAGCGTTTTG GTTTTTCGGA CAAGCAGATA GCTAAGTACA TTAAGAGCAC GGAGCTGGCT 
GTTCGTCACC AGCGGCAGGA GTTTGGCATC CGCCCGCATG TCAAGCAGAT CGACACGGTG 
GCCGGCGAAT GGCCCGCCAG TACCAACTAT CTGTACCACA CATATAACGG AAGCGAGCAC 
GATGTGGACT TCCCCGGCGG ACACACTATT GTGGTGGGCT CGGGCGTCTA TCGTATCGGA 
TCCTCAGTGG AGTTTGATTG GTGTGCCGTG GGCTGCCTGC GCGAATTGAG GAAGCTACAG 
CGACCCACCA TCATGATTAA CTACAATCCT GAAACGGTTT CCACCGACTA CGATATGTGC 
GATCGCTTGT ATTTCGAGGA GATTAGCTTC GAGGTGGTCA TGGACATCTA CGAGATGGAG 
AATAGCGAGG GCATCATTCT GTCCATGGGC GGCCAGCTGC CCAACAATAT AGCCATGGAT 
CTGCATCGCC AGCAGGCGAA GGTGTTGGGC ACATCGCCGG AGTCCATCGA CTGTGCCGAA 
AATCGATTTA AATTCTCGCG CATGCTGGAC AGGAAGGGTA TCTTGCAGCC ACGCTGGAAG 
GAGCTGACCA ACCTACAGTC GGCCATCGAA TTTTGCGAGG AGGTTGGCTA TCCCTGTCTG 
GTTAGGCCAT CCTATGTGCT CTCCGGTGCG GCCATGAATG TGGCCTATTC CAATCAGGAT 
CTGGAGACAT ATCTGAACGC CGCCTCCGAG GTGAGTCGCG AGCATCCGGT TGTCATTTCG 
AAGTTCCTCA CCGAGGCCAA GGAGATCGAT GTGGATGCAG TGGCATCCGA TGGACGCATC 
CTGTGCATGG CTGTTTCGGA GCACGTGGAG AATGCCGGAG TGCATTCAGG TGATGCCACG 
CTGGTTACCC CACCGCAGGA TCTGAATGCG GAGACCCTGG AGGCCATTAA ACGGATCACA 
TGCGATTTGG CCAGCGTACT GGATGTCACC GGTCCCTTCA ACATGCAGCT AATTGCCAAG 
AACAACGAGC TGAAGGTCAT CGAATGCAAT GTCCGTGTGT CGCGATCCTT CCCCTTCGTC 
TCCAAGACGC TGGACCACGA CTTTGTGGCC ACGGCGACGC GTGCGATTGT GGGACTCGAT 
GTGGAGCCAC TGGATGTGCT CCATGGCGTC GGCAAGGTGG GCGTCAAGGT GCCACAGTTC 
AGTTTCTCGC GGCTCGCGGG CGCGGATGTA CAGCTGGGCG TCGAGATGGC CTCCACCGGT 
GAGGTTGCCT GCTTCGGGGA CAACCGCTAT GAGGCGTATC TCAAGGCCAT GATGTCCACG 
GGCTTCCAGA TACCCAAGAA CGCCGTACTT CTCTCCATTG GCAGTTTCAA GCACAAGATG 
GAGCTGCTGC CTTCCATACG GGATCTGGCC AAGATGGGTT ACAAGCTATA CGCTTCCATG 
GGCACTGGCG ACTTTTATGC CGAACACGGA GTTAATGTGG AATCCGTGCA GTGGACATTC 
GACAAGACGA CTCCCGATGA CATCAACGGT GAGCTGAGAC ACCTGGCCGA GTTCCTGGCC 
AACAAGCAGT TCGACCTGGT CATCAACCTC CCGATGAGCG GCGGGGGTGC CAGGCGGGTC 
TCCTCGTTCA TGACCCATGG CTACCGCACC CGTCGCCTGG CCGTGGACTA CTCCATTCCG 
CTGGTGACCG ATGTGAAGTG CACCAAGCTT CTGGTAGAAT CAATGCGAAT GAACGGTGGC 
AAGCCGCCAA TGAAGACGCA CACGGATTGT ATGACCTCGC GTAGAATTGT GAAGCTGCCG 
GGCTTCATCG ATGTGCATGT GCATTTGCGG GAGCCGGGAG CCACGCACAA GGAGGACTTT 
GCCAGTGGAA CAGCAGCCGC TCTGGCTGGC GGCGTGACCC TCGTGTGCGC CATGCCCAAT 
ACAAATCCCT CGATCGTGGA CAGAGAGACG TTCACCCAGT TCCAGGAGCT GGCCAAAGCG 
GGAGCTCGAT GCGACTATGC CCTGTATGTG GGGGCCTCGG ATGACAATTG GGCGCAGGTC 
AACGAGCTGG CCAGTCATGC GTGTGGCCTT AAGATGTACT TAAACGACAC TTTCGGCACC 
CTAAAACTGA GCGACATGAC CAGCTGGCAG AGGCATCTCA GCCACTGGCC CAAGCGGTCT 
CCCATCGTTT GTCATGCGGA GAGGCAGAGC ACAGCGGCTG TGATTATGCT GGCCCATCTT 
CTCGACCGTT CGGTTCACAT CTGCCATGTG GCGCGAAAGG AGGAGATCCA ATTGATTCGT 
TCCGCCAAGG AAAAGGGCGT CAAGGTGACC TGCGAGGTGT GCCCCCATCA CTTGTTCCTC 
AGCACCAAGG ATGTGGAGCG TTTGGGTCAC GGAATGTCCG AGGTAAGGCC ATTGCTCTGC 
TCACCGGAGG ATCAAGAGGC TCTGTGGGAG AATATTGATT ACATCGATGT ATTTGCCACC 
GATCACGCGC CACACACACT GGCCGAGAAA CGTTCGGAGC GTCCACCGCC TGGATTTCCG 
GGCGTGGAGA CCATTCTGCC GCTCCTCCTG CAGGCTGTTC ACGAGGGTCG TTTGACGATG 
GAGGACATCA AGAGGAAGTT CCATCGCAAT CCAAAAATCA TCTTCAACCT GCCGGATCAG 
GCGCAGACCT ACGTGGAGGT GGATCTCGAC GAGGAGTGGA CCATAACGGG CAATGAGATG 
AAGAGCAAGT CCGGCTGGAC ACCATTTGAG GGCACCAAGG TCAAGGGACG CGTCCACCGG 
GTGGTTCTTC GCGGCGAGGT GGCCTTCGTC GATGGCCAAG TGTTGGTGCA GCCCGGCTTC 
GGGCAGAATG TGCGCCCCAA ACAGAGTCCA TTGGCGTCGG AGGCATCACA GGATCTGCTG 
CCCAGCGACA ACGATGCCAA TGACACCTTT ACCCGCCTCC TCACCTCCGA AGGACCCGGT 
GGAGGCGTAC ATGGAATATC GACCAAGGTG CACTTTGTGG ACGGAGCCAA CTTCCTGCGC 
CCCAACTCGC CATCCCCACG CATCCGTCTG GATTCCGCCA GCAACACCAC GCTGCGGGAA 
TACTTGCAGC GCACTACCAA CTCAAATCCG GTGGCCCACT CGCTAATGGG CAAGCACATC 
CTTGCCGTTG ACATGTTCAA CAAAGATCAC CTCAACGACA TCTTTAACCT GGCACAGCTG 
CTCAAGCTGC GGGGTACGAA GGATCGTCCG GTGGACGAGC TGCTGCCAGG CAAGATCATG 
GCCAGTGTGT TTTACGAGGT CAGCACGCGG ACGCAGTGCA GCTTTGCAGC TGCCATGCTG 
CGTTTGGGAG GGCGAGTGAT CAGCATGGAC AACATCACGT CGTCGGTGAA GAAGGGCGAG 
AGCCTGGAGG ACAGCATCAA GGTTGTGTCC AGCTATGCCG ACGTCGTGGT GCTCCGTCAT 
CCATCGCCCG GAGCTGTAGC GCGCGCAGCG ACCTTCTCCC GAAAGCCGCT TATCAATGCC 
GGCGATGGCG TCGGTGAGCA TCCCACGCAG GCGCTGCTGG ACATCTTCAC CATCCGCGAG 
GAGTTTGGCA CTGTGAACGG GCTGACCATC ACCATGGTGG GCGACTTGAA GAACGGACGG 
ACCGTGCACT CGCTGGCCCG CCTGCTGACC CTGTACAATG TGAACCTGCA GTATGTGGCG 
CCGAATAGCC TGCAGATGCC CGATGAGGTC GTCCAGTTCG TCCATCAGCG CGGCGTCAAG 
CAGTTATTTG CACGCGACCT AAAGAATGTG CTGCCCGACA CGGATGTGCT CTACATGACT 
CGCATTCAGC GCGAGCGTTT TGATAACGTG GAAGATTACG AGAAGTGCTG TGGCCATCTG 
GTGTTGACGC CCGAGCATAT GATGCGGGCC AAGAAGCGTT CGATTGTTCT GCATCCGCTG 
CCGCGTCTGA ACGAGATCAG CCGGGAGATC GACTCGGACC CAAGGGCCGC CTACTTCCGG 
CAGGCGGAGT ACGGCATGTA CATCCGTATG GCCTTGCTGG CCATGGTCGT TGGTGGACGC 
AATACGGCGC TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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r cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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