TOPII cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOPII
Entrez Gene ID	821972
Full Name	topoisomerase II
Synonyms	ATTOPII,TOPOISOMERASE II,topoisomerase II
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_113294.3	NP_189031.1	topoisomerase II
NM_001125214.2	NP_001118686.1	topoisomerase II

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	TOP2	NP_014311.3
Schizosaccharomyces pombe (fission yeast)	top2	NP_595805.1
Oryza sativa (rice)	Os02g0699700	NP_001047833.1
Pan troglodytes (chimpanzee)	TOP2B	XP_516332.3
	TOP2B	XP_001092092.2
Gallus gallus (chicken)	TOP2B	NP_990413.1
Neurospora crassa	NCU06338	XP_962372.2
Danio rerio (zebrafish)	top2b	NP_001038656.1
Bos taurus (cattle)	TOP2B	XP_002698796.2
Mus musculus (house mouse)	Top2b	NP_033435.2
Magnaporthe oryzae (rice blast fungus)	MGG_06162	XP_003712042.1
Arabidopsis thaliana (thale cress)	TOPII	NP_001118686.1
Homo sapiens (human)	TOP2B	NP_001059.2
Canis lupus familiaris (dog)	TOP2B	XP_534241.3
Rattus norvegicus (Norway rat)	Top2b	NP_001094328.1
Kluyveromyces lactis	KLLA0C13838g	XP_452818.1
Eremothecium gossypii	AGOS_AER410W	NP_985265.1
Xenopus tropicalis (tropical clawed frog)	top2b	XP_004915443.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TOPII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb12271	NM_001125214.2 Latest version!	Arabidopsis thaliana topoisomerase II (TOPII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OAb12270	NM_113294.3 Latest version!	Arabidopsis thaliana topoisomerase II (TOPII), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOPII ( NM_001125214.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001118686.1 Arabidopsis thaliana topoisomerase II (TOPII), mRNA.

Price & Availability↑

CloneID	OAb12271
Clone ID Related Accession (Same CDS sequence)	NM_001125214.2
Accession Version	NM_001125214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	topoisomerase II
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001125214.1.

ATGGCTACCA AACTTCCCCT TCAAAACAGC AATGCCGCCA ATGTTGCGAA AGCTCCGGCT 
AAATCTCGAG CTGCCGCCGG CGGGAAGACG ATCGAGGAAA TGTATCAGAA GAAATCGCAG 
CTTGAACACA TTCTTCTCCG TCCTGATACA TACATCGGAT CTATCGAGAA ACACACCCAA 
ACACTTTGGG TTTATGAGAA AGATGAGATG GTTCAACGTC CTGTTACCTA TGTACCTGGA 
TTGTATAAGA TCTTTGATGA GATTCTCGTA AACGCCGCTG ATAACAAACA GAGAGATGCG 
AAGATGGATT CCGTTCAAGT TGTGATTGAT GTTGAGCAGA ATCTGATTAG TGTTTGTAAC 
AGTGGAGCTG GAGTTCCTGT AGAGATTCAT CAGGAAGAAG GAATTTATGT ACCGGAGATG 
ATATTTGGTC ATTTGTTGAC GAGTAGTAAC TACGATGATA ATGTGAAGAA GACCACTGGT 
GGAAGAAACG GATATGGTGC TAAGCTTACT AATATCTTCT CTACTGAATT CATCATCGAG 
ACTGCAGATG GGAAGAGACT CAAGAAGTAT AAGCAGGTCT TTGAGAACAA CATGGGGAAG 
AAATCTGAAC CGGTTATAAC CAAGTGTAAT AAGAGTGAGA ACTGGACGAA GGTTACATTC 
AAACCAGATT TGAAGAAGTT TAACATGACT GAATTAGAAG ACGATGTGGT TGCTCTAATG 
AGTAAGCGAG TGTTTGATAT TGCTGGATGT TTGGGGAAGT CTGTCAAAGT TGAGCTGAAT 
GGGAAACAAA TACCAGTGAA GTCATTCACT GATTATGTTG ACCTATACCT AAGTGCAGCT 
AATAAATCGA GGACGGAGGA TCCTCTCCCA AGGTTGACTG AGAAAGTTAA TGATAGATGG 
GAGGTTTGTG TGAGCTTAAG TGAAGGACAA TTTCAGCAGG TCAGCTTTGT CAACTCCATA 
GCAACAATCA AGGGTGGTAC ACATGTTGAC TATGTGACGA GTCAGATTAC AAACCATATT 
GTGGCTGCTG TGAACAAAAA GAATAAAAAC GCAAATGTTA AGGCCCATAA TGTGAAAAAC 
CATCTGTGGG TCTTTGTCAA TGCATTGATT GACAATCCTG CTTTTGATTC TCAAACAAAA 
GAAACACTGA CTCTTCGACA GAGCAGTTTT GGGTCGAAAT GTGAACTTTC AGAAGATTTT 
CTGAAGAAAG TTGGAAAATC TGGCGTAGTG GAGAATTTGC TTTCATGGGC AGATTTCAAG 
CAGAACAAAG ATCTAAAGAA AAGTGATGGA GCCAAAACAG GGAGGGTTCT AGTTGAGAAG 
TTAGACGATG CCGCCGAAGC TGGAGGGAAG AACTCTCGAC TCTGTACGTT AATTTTGACT 
GAAGGAGACT CAGCAAAATC TCTCGCTTTG GCGGGGAGGT CTGTCTTGGG TAATAACTAC 
TGCGGTGTGT TTCCGCTTCG AGGAAAGCTA TTGAATGTCA GGGAAGCCAG CACCACTCAG 
ATTACAAACA ATAAGGAAAT TGAGAACCTC AAGAAGATCC TTGGACTAAA GCAAAATATG 
AAGTACGAGA ATGTTAACTC ATTAAGATAT GGCCAGATGA TGATCATGAC TGATCAAGAT 
CATGATGGTT CTCATATAAA GGGTCTCTTA ATCAATTTTA TCCACTCTTT TTGGCCGTCG 
TTACTCCAGG TTCCATCATT CCTGGTTGAG TTTATAACAC CCATTGTGAA GGCTACGCGT 
AAGGGTACAA AAAAAGTTCT GTCATTCTAC TCAATGCCTG AATACGAAGA ATGGAAAGAA 
AGTCTGAAGG GTAATGCAAC CGGGTGGGAT ATCAAATACT ACAAGGGGTT GGGTACAAGT 
ACGGCTGAGG AGGGCAAAGA GTACTTTAGT AATCTTGGTC TTCACAAAAA GGATTTTGTT 
TGGGAGGATG AACAAGATGG AGAAGCCATT GAACTTGCTT TCAGTAAAAA GAAGATCGAA 
GCCCGGAAAA ATTGGCTTTC GAGCTATGTG CCTGGCAATC ATCTTGATCA GAGACAACCG 
AAAGTTACGT ACAGTGACTT CGTGAACAAA GAACTGATCT TGTTCTCCAT GGCGGATCTT 
CAACGGTCAA TTCCCTCAAT GGTTGATGGG CTCAAACCGG GTCAGAGAAA GATACTTTTC 
GTTGCATTCA AGAAAATCGC TCGTAAAGAA ATGAAGGTTG CCCAGCTCGT TGGTTATGTA 
TCATTGCTGT CAGCTTATCA TCATGGCGAG CAGAGTCTTG CCAGTGCTAT CATAGGCATG 
GCGCAGGATT ACGTGGGCAG CAACAATATC AACTTACTTC TACCAAATGG TCAATTTGGT 
ACACGAACTT CGGGTGGAAA AGATTCAGCT AGTGCAAGAT ACATTTTCAC TAAGCTCTCT 
CCAGTAACAA GGATCTTGTT TCCCAAGGAT GATGATCTCC TTCTTGATTA CTTGAATGAA 
GACGGGCAAA GGATAGAGCC AACTTGGTAC ATGCCCATCA TTCCAACTGT ACTTGTCAAT 
GGCGCTGAAG GCATTGGAAC TGGGTGGAGC ACGTTCATAC CAAACTATAA CCCTAGAGAG 
ATCGTTGCTA ATGTAAGGCG TTTACTTAAC GGTGAAAGTA TGGTGCCTAT GGATCCTTGG 
TATAGAGGTT TTAAAGGAAC AATTGAGAAA ACTGCGTCCA AAGAAGGTGG TTGTACTTAC 
ACAATCACTG GTCTATATGA GGAGGTGGAT GAAACAACTA TTCGAATCAC GGAGTTGCCA 
ATCAGAAGGT GGAATGATGA TTACAAAAAT TTCTTGCAGT CATTGAAGAC AGATAATGGT 
GCACCTTTTT TCCAGGATGT CAAGGCTTAC AATGACGAGA AATCTGTTGA TTTTGACCTT 
ATTTTGAGTG AAGAGAACAT GCTGGCGGCT CGGCAAGAGG GCTTTCTGAA GAAATTCAAA 
CTCACCACGA CAATCGCTAC AAGTAATATG CATCTCTTTG ATAAAAAAGG TGTGATAAAG 
AAATATGTGA CCCCAGAACA AATCCTTGAA GAGTTCTTTG ACCTCAGGTT TGAATACTAT 
GAGAAGAGAA AGGAAACCGT GGTGAAGAAT ATGGAAATTG AGCTGTTGAA ACTAGAAAAC 
AAAGCGAGGT TTATTCTTGC AGTTCTCAGT GGAGAAATCA TAGTGAACAA GAGGAAGAAA 
GCCGATATTG TTGAGGACTT AAGACAGAAA GGGTTCACAC CATTTCCGAG GAAGGCCGAG 
TCTGTCGAAG CTGCAATTGC TGGAGCAGTT GATGATGATG CAGCTGAAGA ACCTGAAGAA 
ATCTTAGTGG ATCCGGAGTC AAGCTCCAGT TATATACCCG GCTCGGAATA TGACTACCTA 
TTGGCAATGG CTATAGCTTC GTTGACTATA GAGAAAGTTG AGGAGTTATT AGCAGACAGA 
GATAAAATGA TTATTGCAGT TGCAGACATG AAGAAAACCA CACCGAAATC CCTCTGGCTC 
AGTGACCTAG AGTCTCTTGA CAAGGAACTA GAAAAACTTG ATCTGAAAGA TGCTCAAGTC 
CAACAGGCTA TAGAGGCAGC ACAGAAAAAA ATCAGGGCCA AATCTGGAGC AGCAGTAAAG 
GTTAAAAGGC AGGCACCAAA GAAACCAGCA CCAAAGAAGA CCACAAAGAA AGCCAGTGAA 
TCTGAGACAA CAGAGGCTTC CTATTCAGCA ATGGACACAG ATAACAACGT AGCAGAAGTG 
GTGAAGCCAA AAGGTAGACA AGGAGCAAAG AAGAAGGCTC CAGCAGCTGC GAAGGAGGTT 
GAGGAGGATG AAATGTTGGA TCTAGCACAA CGGCTTGCTC AATACAATTT CGGCTCAGCA 
CCTGCGGATT CAAGCAAAAC AGCTGAAACA TCGAAAGCAA TTGCTGTGGA TGACGATGAT 
GATGACGTGG TAGTTGAGGT TGCACCAGTA AAGAAAGGAG GAAGGAAACC GGCGGCGACT 
AAAGCTGCAA AACCACCAGC AGCTCCGAGG AAACGAGGGA AGCAGACAGT TGCTTCAACA 
GAGGTTCTGG CAATTGGTGT GTCCCCGGAG AAGAAAGTGA GGAAGATGAG ATCATCTCCG 
TTCAACAAGA AGAGTAGTTC AGTGATGAGC AGGTTGGCTG ATAATAAGGA AGAGGAGAGC 
AGCGAGAATG TGGCTGGAAA CTCATCGTCT GAGAAATCTG GAGGAGATGT TTCTGCGATA 
TCGAGGCCTC AAAGAGCTAA TAGGAGGAAA ATGACCTATG TGTTGAGTGA TTCCGAGAGT 
GAATCTGCGA ATGATTCTGA GTTTGATGAC ATTGAAGACG ATGAAGATGA TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001118686.1
CDS	286..4602
Translation	MATKLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLWVYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGAGVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKRVFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVCVSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLWVFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWADFKQNKDLKKSDGAKTGRVLVEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYCGVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKATRKGTKKVLSFYSMPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIEARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFVAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTYTITGLYEEVDETTIRITELPIRRWNDDYKNFLQSLKTDNGAPFFQDVKAYNDEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEEFFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKGFTPFPRKAESVEAAIAGAVDDDAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTIEKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKKIRAKSGAAVKVKRQAPKKPAPKKTTKKASESETTEASYSAMDTDNNVAEVVKPKGRQGAKKKAPAAAKEVEEDEMLDLAQRLAQYNFGSAPADSSKTAETSKAIAVDDDDDDVVVEVAPVKKGGRKPAATKAAKPPAAPRKRGKQTVASTEVLAIGVSPEKKVRKMRSSPFNKKSSSVMSRLADNKEEESSENVAGNSSSEKSGGDVSAISRPQRANRRKMTYVLSDSESESANDSEFDDIEDDEDDE

Target ORF information:

RefSeq Version	NM_001125214.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana topoisomerase II (TOPII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001125214.2

ORF Insert Sequence:

ATGGCTACCA AACTTCCCCT TCAAAACAGC AATGCCGCCA ATGTTGCGAA AGCTCCGGCT 
AAATCTCGAG CTGCCGCCGG CGGGAAGACG ATCGAGGAAA TGTATCAGAA GAAATCGCAG 
CTTGAACACA TTCTTCTCCG TCCTGATACA TACATCGGAT CTATCGAGAA ACACACCCAA 
ACACTTTGGG TTTATGAGAA AGATGAGATG GTTCAACGTC CTGTTACCTA TGTACCTGGA 
TTGTATAAGA TCTTTGATGA GATTCTCGTA AACGCCGCTG ATAACAAACA GAGAGATGCG 
AAGATGGATT CCGTTCAAGT TGTGATTGAT GTTGAGCAGA ATCTGATTAG TGTTTGTAAC 
AGTGGAGCTG GAGTTCCTGT AGAGATTCAT CAGGAAGAAG GAATTTATGT ACCGGAGATG 
ATATTTGGTC ATTTGTTGAC GAGTAGTAAC TACGATGATA ATGTGAAGAA GACCACTGGT 
GGAAGAAACG GATATGGTGC TAAGCTTACT AATATCTTCT CTACTGAATT CATCATCGAG 
ACTGCAGATG GGAAGAGACT CAAGAAGTAT AAGCAGGTCT TTGAGAACAA CATGGGGAAG 
AAATCTGAAC CGGTTATAAC CAAGTGTAAT AAGAGTGAGA ACTGGACGAA GGTTACATTC 
AAACCAGATT TGAAGAAGTT TAACATGACT GAATTAGAAG ACGATGTGGT TGCTCTAATG 
AGTAAGCGAG TGTTTGATAT TGCTGGATGT TTGGGGAAGT CTGTCAAAGT TGAGCTGAAT 
GGGAAACAAA TACCAGTGAA GTCATTCACT GATTATGTTG ACCTATACCT AAGTGCAGCT 
AATAAATCGA GGACGGAGGA TCCTCTCCCA AGGTTGACTG AGAAAGTTAA TGATAGATGG 
GAGGTTTGTG TGAGCTTAAG TGAAGGACAA TTTCAGCAGG TCAGCTTTGT CAACTCCATA 
GCAACAATCA AGGGTGGTAC ACATGTTGAC TATGTGACGA GTCAGATTAC AAACCATATT 
GTGGCTGCTG TGAACAAAAA GAATAAAAAC GCAAATGTTA AGGCCCATAA TGTGAAAAAC 
CATCTGTGGG TCTTTGTCAA TGCATTGATT GACAATCCTG CTTTTGATTC TCAAACAAAA 
GAAACACTGA CTCTTCGACA GAGCAGTTTT GGGTCGAAAT GTGAACTTTC AGAAGATTTT 
CTGAAGAAAG TTGGAAAATC TGGCGTAGTG GAGAATTTGC TTTCATGGGC AGATTTCAAG 
CAGAACAAAG ATCTAAAGAA AAGTGATGGA GCCAAAACAG GGAGGGTTCT AGTTGAGAAG 
TTAGACGATG CCGCCGAAGC TGGAGGGAAG AACTCTCGAC TCTGTACGTT AATTTTGACT 
GAAGGAGACT CAGCAAAATC TCTCGCTTTG GCGGGGAGGT CTGTCTTGGG TAATAACTAC 
TGCGGTGTGT TTCCGCTTCG AGGAAAGCTA TTGAATGTCA GGGAAGCCAG CACCACTCAG 
ATTACAAACA ATAAGGAAAT TGAGAACCTC AAGAAGATCC TTGGACTAAA GCAAAATATG 
AAGTACGAGA ATGTTAACTC ATTAAGATAT GGCCAGATGA TGATCATGAC TGATCAAGAT 
CATGATGGTT CTCATATAAA GGGTCTCTTA ATCAATTTTA TCCACTCTTT TTGGCCGTCG 
TTACTCCAGG TTCCATCATT CCTGGTTGAG TTTATAACAC CCATTGTGAA GGCTACGCGT 
AAGGGTACAA AAAAAGTTCT GTCATTCTAC TCAATGCCTG AATACGAAGA ATGGAAAGAA 
AGTCTGAAGG GTAATGCAAC CGGGTGGGAT ATCAAATACT ACAAGGGGTT GGGTACAAGT 
ACGGCTGAGG AGGGCAAAGA GTACTTTAGT AATCTTGGTC TTCACAAAAA GGATTTTGTT 
TGGGAGGATG AACAAGATGG AGAAGCCATT GAACTTGCTT TCAGTAAAAA GAAGATCGAA 
GCCCGGAAAA ATTGGCTTTC GAGCTATGTG CCTGGCAATC ATCTTGATCA GAGACAACCG 
AAAGTTACGT ACAGTGACTT CGTGAACAAA GAACTGATCT TGTTCTCCAT GGCGGATCTT 
CAACGGTCAA TTCCCTCAAT GGTTGATGGG CTCAAACCGG GTCAGAGAAA GATACTTTTC 
GTTGCATTCA AGAAAATCGC TCGTAAAGAA ATGAAGGTTG CCCAGCTCGT TGGTTATGTA 
TCATTGCTGT CAGCTTATCA TCATGGCGAG CAGAGTCTTG CCAGTGCTAT CATAGGCATG 
GCGCAGGATT ACGTGGGCAG CAACAATATC AACTTACTTC TACCAAATGG TCAATTTGGT 
ACACGAACTT CGGGTGGAAA AGATTCAGCT AGTGCAAGAT ACATTTTCAC TAAGCTCTCT 
CCAGTAACAA GGATCTTGTT TCCCAAGGAT GATGATCTCC TTCTTGATTA CTTGAATGAA 
GACGGGCAAA GGATAGAGCC AACTTGGTAC ATGCCCATCA TTCCAACTGT ACTTGTCAAT 
GGCGCTGAAG GCATTGGAAC TGGGTGGAGC ACGTTCATAC CAAACTATAA CCCTAGAGAG 
ATCGTTGCTA ATGTAAGGCG TTTACTTAAC GGTGAAAGTA TGGTGCCTAT GGATCCTTGG 
TATAGAGGTT TTAAAGGAAC AATTGAGAAA ACTGCGTCCA AAGAAGGTGG TTGTACTTAC 
ACAATCACTG GTCTATATGA GGAGGTGGAT GAAACAACTA TTCGAATCAC GGAGTTGCCA 
ATCAGAAGGT GGAATGATGA TTACAAAAAT TTCTTGCAGT CATTGAAGAC AGATAATGGT 
GCACCTTTTT TCCAGGATGT CAAGGCTTAC AATGACGAGA AATCTGTTGA TTTTGACCTT 
ATTTTGAGTG AAGAGAACAT GCTGGCGGCT CGGCAAGAGG GCTTTCTGAA GAAATTCAAA 
CTCACCACGA CAATCGCTAC AAGTAATATG CATCTCTTTG ATAAAAAAGG TGTGATAAAG 
AAATATGTGA CCCCAGAACA AATCCTTGAA GAGTTCTTTG ACCTCAGGTT TGAATACTAT 
GAGAAGAGAA AGGAAACCGT GGTGAAGAAT ATGGAAATTG AGCTGTTGAA ACTAGAAAAC 
AAAGCGAGGT TTATTCTTGC AGTTCTCAGT GGAGAAATCA TAGTGAACAA GAGGAAGAAA 
GCCGATATTG TTGAGGACTT AAGACAGAAA GGGTTCACAC CATTTCCGAG GAAGGCCGAG 
TCTGTCGAAG CTGCAATTGC TGGAGCAGTT GATGATGATG CAGCTGAAGA ACCTGAAGAA 
ATCTTAGTGG ATCCGGAGTC AAGCTCCAGT TATATACCCG GCTCGGAATA TGACTACCTA 
TTGGCAATGG CTATAGCTTC GTTGACTATA GAGAAAGTTG AGGAGTTATT AGCAGACAGA 
GATAAAATGA TTATTGCAGT TGCAGACATG AAGAAAACCA CACCGAAATC CCTCTGGCTC 
AGTGACCTAG AGTCTCTTGA CAAGGAACTA GAAAAACTTG ATCTGAAAGA TGCTCAAGTC 
CAACAGGCTA TAGAGGCAGC ACAGAAAAAA ATCAGGGCCA AATCTGGAGC AGCAGTAAAG 
GTTAAAAGGC AGGCACCAAA GAAACCAGCA CCAAAGAAGA CCACAAAGAA AGCCAGTGAA 
TCTGAGACAA CAGAGGCTTC CTATTCAGCA ATGGACACAG ATAACAACGT AGCAGAAGTG 
GTGAAGCCAA AAGGTAGACA AGGAGCAAAG AAGAAGGCTC CAGCAGCTGC GAAGGAGGTT 
GAGGAGGATG AAATGTTGGA TCTAGCACAA CGGCTTGCTC AATACAATTT CGGCTCAGCA 
CCTGCGGATT CAAGCAAAAC AGCTGAAACA TCGAAAGCAA TTGCTGTGGA TGACGATGAT 
GATGACGTGG TAGTTGAGGT TGCACCAGTA AAGAAAGGAG GAAGGAAACC GGCGGCGACT 
AAAGCTGCAA AACCACCAGC AGCTCCGAGG AAACGAGGGA AGCAGACAGT TGCTTCAACA 
GAGGTTCTGG CAATTGGTGT GTCCCCGGAG AAGAAAGTGA GGAAGATGAG ATCATCTCCG 
TTCAACAAGA AGAGTAGTTC AGTGATGAGC AGGTTGGCTG ATAATAAGGA AGAGGAGAGC 
AGCGAGAATG TGGCTGGAAA CTCATCGTCT GAGAAATCTG GAGGAGATGT TTCTGCGATA 
TCGAGGCCTC AAAGAGCTAA TAGGAGGAAA ATGACCTATG TGTTGAGTGA TTCCGAGAGT 
GAATCTGCGA ATGATTCTGA GTTTGATGAC ATTGAAGACG ATGAAGATGA TGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPII ( NM_113294.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_189031.1 Arabidopsis thaliana topoisomerase II (TOPII), mRNA.

Price & Availability↑

CloneID	OAb12270
Clone ID Related Accession (Same CDS sequence)	NM_113294.3
Accession Version	NM_113294.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	topoisomerase II
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_113294.2.

ATGGCTACCA AACTTCCCCT TCAAAACAGC AATGCCGCCA ATGTTGCGAA AGCTCCGGCT 
AAATCTCGAG CTGCCGCCGG CGGGAAGACG ATCGAGGAAA TGTATCAGAA GAAATCGCAG 
CTTGAACACA TTCTTCTCCG TCCTGATACA TACATCGGAT CTATCGAGAA ACACACCCAA 
ACACTTTGGG TTTATGAGAA AGATGAGATG GTTCAACGTC CTGTTACCTA TGTACCTGGA 
TTGTATAAGA TCTTTGATGA GATTCTCGTA AACGCCGCTG ATAACAAACA GAGAGATGCG 
AAGATGGATT CCGTTCAAGT TGTGATTGAT GTTGAGCAGA ATCTGATTAG TGTTTGTAAC 
AGTGGAGCTG GAGTTCCTGT AGAGATTCAT CAGGAAGAAG GAATTTATGT ACCGGAGATG 
ATATTTGGTC ATTTGTTGAC GAGTAGTAAC TACGATGATA ATGTGAAGAA GACCACTGGT 
GGAAGAAACG GATATGGTGC TAAGCTTACT AATATCTTCT CTACTGAATT CATCATCGAG 
ACTGCAGATG GGAAGAGACT CAAGAAGTAT AAGCAGGTCT TTGAGAACAA CATGGGGAAG 
AAATCTGAAC CGGTTATAAC CAAGTGTAAT AAGAGTGAGA ACTGGACGAA GGTTACATTC 
AAACCAGATT TGAAGAAGTT TAACATGACT GAATTAGAAG ACGATGTGGT TGCTCTAATG 
AGTAAGCGAG TGTTTGATAT TGCTGGATGT TTGGGGAAGT CTGTCAAAGT TGAGCTGAAT 
GGGAAACAAA TACCAGTGAA GTCATTCACT GATTATGTTG ACCTATACCT AAGTGCAGCT 
AATAAATCGA GGACGGAGGA TCCTCTCCCA AGGTTGACTG AGAAAGTTAA TGATAGATGG 
GAGGTTTGTG TGAGCTTAAG TGAAGGACAA TTTCAGCAGG TCAGCTTTGT CAACTCCATA 
GCAACAATCA AGGGTGGTAC ACATGTTGAC TATGTGACGA GTCAGATTAC AAACCATATT 
GTGGCTGCTG TGAACAAAAA GAATAAAAAC GCAAATGTTA AGGCCCATAA TGTGAAAAAC 
CATCTGTGGG TCTTTGTCAA TGCATTGATT GACAATCCTG CTTTTGATTC TCAAACAAAA 
GAAACACTGA CTCTTCGACA GAGCAGTTTT GGGTCGAAAT GTGAACTTTC AGAAGATTTT 
CTGAAGAAAG TTGGAAAATC TGGCGTAGTG GAGAATTTGC TTTCATGGGC AGATTTCAAG 
CAGAACAAAG ATCTAAAGAA AAGTGATGGA GCCAAAACAG GGAGGGTTCT AGTTGAGAAG 
TTAGACGATG CCGCCGAAGC TGGAGGGAAG AACTCTCGAC TCTGTACGTT AATTTTGACT 
GAAGGAGACT CAGCAAAATC TCTCGCTTTG GCGGGGAGGT CTGTCTTGGG TAATAACTAC 
TGCGGTGTGT TTCCGCTTCG AGGAAAGCTA TTGAATGTCA GGGAAGCCAG CACCACTCAG 
ATTACAAACA ATAAGGAAAT TGAGAACCTC AAGAAGATCC TTGGACTAAA GCAAAATATG 
AAGTACGAGA ATGTTAACTC ATTAAGATAT GGCCAGATGA TGATCATGAC TGATCAAGAT 
CATGATGGTT CTCATATAAA GGGTCTCTTA ATCAATTTTA TCCACTCTTT TTGGCCGTCG 
TTACTCCAGG TTCCATCATT CCTGGTTGAG TTTATAACAC CCATTGTGAA GGCTACGCGT 
AAGGGTACAA AAAAAGTTCT GTCATTCTAC TCAATGCCTG AATACGAAGA ATGGAAAGAA 
AGTCTGAAGG GTAATGCAAC CGGGTGGGAT ATCAAATACT ACAAGGGGTT GGGTACAAGT 
ACGGCTGAGG AGGGCAAAGA GTACTTTAGT AATCTTGGTC TTCACAAAAA GGATTTTGTT 
TGGGAGGATG AACAAGATGG AGAAGCCATT GAACTTGCTT TCAGTAAAAA GAAGATCGAA 
GCCCGGAAAA ATTGGCTTTC GAGCTATGTG CCTGGCAATC ATCTTGATCA GAGACAACCG 
AAAGTTACGT ACAGTGACTT CGTGAACAAA GAACTGATCT TGTTCTCCAT GGCGGATCTT 
CAACGGTCAA TTCCCTCAAT GGTTGATGGG CTCAAACCGG GTCAGAGAAA GATACTTTTC 
GTTGCATTCA AGAAAATCGC TCGTAAAGAA ATGAAGGTTG CCCAGCTCGT TGGTTATGTA 
TCATTGCTGT CAGCTTATCA TCATGGCGAG CAGAGTCTTG CCAGTGCTAT CATAGGCATG 
GCGCAGGATT ACGTGGGCAG CAACAATATC AACTTACTTC TACCAAATGG TCAATTTGGT 
ACACGAACTT CGGGTGGAAA AGATTCAGCT AGTGCAAGAT ACATTTTCAC TAAGCTCTCT 
CCAGTAACAA GGATCTTGTT TCCCAAGGAT GATGATCTCC TTCTTGATTA CTTGAATGAA 
GACGGGCAAA GGATAGAGCC AACTTGGTAC ATGCCCATCA TTCCAACTGT ACTTGTCAAT 
GGCGCTGAAG GCATTGGAAC TGGGTGGAGC ACGTTCATAC CAAACTATAA CCCTAGAGAG 
ATCGTTGCTA ATGTAAGGCG TTTACTTAAC GGTGAAAGTA TGGTGCCTAT GGATCCTTGG 
TATAGAGGTT TTAAAGGAAC AATTGAGAAA ACTGCGTCCA AAGAAGGTGG TTGTACTTAC 
ACAATCACTG GTCTATATGA GGAGGTGGAT GAAACAACTA TTCGAATCAC GGAGTTGCCA 
ATCAGAAGGT GGAATGATGA TTACAAAAAT TTCTTGCAGT CATTGAAGAC AGATAATGGT 
GCACCTTTTT TCCAGGATGT CAAGGCTTAC AATGACGAGA AATCTGTTGA TTTTGACCTT 
ATTTTGAGTG AAGAGAACAT GCTGGCGGCT CGGCAAGAGG GCTTTCTGAA GAAATTCAAA 
CTCACCACGA CAATCGCTAC AAGTAATATG CATCTCTTTG ATAAAAAAGG TGTGATAAAG 
AAATATGTGA CCCCAGAACA AATCCTTGAA GAGTTCTTTG ACCTCAGGTT TGAATACTAT 
GAGAAGAGAA AGGAAACCGT GGTGAAGAAT ATGGAAATTG AGCTGTTGAA ACTAGAAAAC 
AAAGCGAGGT TTATTCTTGC AGTTCTCAGT GGAGAAATCA TAGTGAACAA GAGGAAGAAA 
GCCGATATTG TTGAGGACTT AAGACAGAAA GGGTTCACAC CATTTCCGAG GAAGGCCGAG 
TCTGTCGAAG CTGCAATTGC TGGAGCAGTT GATGATGATG CAGCTGAAGA ACCTGAAGAA 
ATCTTAGTGG ATCCGGAGTC AAGCTCCAGT TATATACCCG GCTCGGAATA TGACTACCTA 
TTGGCAATGG CTATAGCTTC GTTGACTATA GAGAAAGTTG AGGAGTTATT AGCAGACAGA 
GATAAAATGA TTATTGCAGT TGCAGACATG AAGAAAACCA CACCGAAATC CCTCTGGCTC 
AGTGACCTAG AGTCTCTTGA CAAGGAACTA GAAAAACTTG ATCTGAAAGA TGCTCAAGTC 
CAACAGGCTA TAGAGGCAGC ACAGAAAAAA ATCAGGGCCA AATCTGGAGC AGCAGTAAAG 
GTTAAAAGGC AGGCACCAAA GAAACCAGCA CCAAAGAAGA CCACAAAGAA AGCCAGTGAA 
TCTGAGACAA CAGAGGCTTC CTATTCAGCA ATGGACACAG ATAACAACGT AGCAGAAGTG 
GTGAAGCCAA AAGCTAGACA AGGAGCAAAG AAGAAAGCCA GTGAATCTGA AACAACAGAG 
GCTTCCCATT CAGCAATGGA CACAGATAAC AACGTAGCAG AAGTGGTGAA GCCAAAAGGT 
AGACAAGGAG CAAAGAAGAA GGCTCCAGCA GCTGCGAAGG AGGTTGAGGA GGATGAAATG 
TTGGATCTAG CACAACGGCT TGCTCAATAC AATTTCGGCT CAGCACCTGC GGATTCAAGC 
AAAACAGCTG AAACATCGAA AGCAATTGCT GTGGATGACG ATGATGATGA CGTGGTAGTT 
GAGGTTGCAC CAGTAAAGAA AGGAGGAAGG AAACCGGCGG CGACTAAAGC TGCAAAACCA 
CCAGCAGCTC CGAGGAAACG AGGGAAGCAG ACAGTTGCTT CAACAGAGGT TCTGGCAATT 
GGTGTGTCCC CGGAGAAGAA AGTGAGGAAG ATGAGATCAT CTCCGTTCAA CAAGAAGAGT 
AGTTCAGTGA TGAGCAGGTT GGCTGATAAT AAGGAAGAGG AGAGCAGCGA GAATGTGGCT 
GGAAACTCAT CGTCTGAGAA ATCTGGAGGA GATGTTTCTG CGATATCGAG GCCTCAAAGA 
GCTAATAGGA GGAAAATGAC CTATGTGTTG AGTGATTCCG AGAGTGAATC TGCGAATGAT 
TCTGAGTTTG ATGACATTGA AGACGATGAA GATGATGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_189031.1
CDS	296..4717
Translation	MATKLPLQNSNAANVAKAPAKSRAAAGGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHTQTLWVYEKDEMVQRPVTYVPGLYKIFDEILVNAADNKQRDAKMDSVQVVIDVEQNLISVCNSGAGVPVEIHQEEGIYVPEMIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGKRLKKYKQVFENNMGKKSEPVITKCNKSENWTKVTFKPDLKKFNMTELEDDVVALMSKRVFDIAGCLGKSVKVELNGKQIPVKSFTDYVDLYLSAANKSRTEDPLPRLTEKVNDRWEVCVSLSEGQFQQVSFVNSIATIKGGTHVDYVTSQITNHIVAAVNKKNKNANVKAHNVKNHLWVFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWADFKQNKDLKKSDGAKTGRVLVEKLDDAAEAGGKNSRLCTLILTEGDSAKSLALAGRSVLGNNYCGVFPLRGKLLNVREASTTQITNNKEIENLKKILGLKQNMKYENVNSLRYGQMMIMTDQDHDGSHIKGLLINFIHSFWPSLLQVPSFLVEFITPIVKATRKGTKKVLSFYSMPEYEEWKESLKGNATGWDIKYYKGLGTSTAEEGKEYFSNLGLHKKDFVWEDEQDGEAIELAFSKKKIEARKNWLSSYVPGNHLDQRQPKVTYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILFVAFKKIARKEMKVAQLVGYVSLLSAYHHGEQSLASAIIGMAQDYVGSNNINLLLPNGQFGTRTSGGKDSASARYIFTKLSPVTRILFPKDDDLLLDYLNEDGQRIEPTWYMPIIPTVLVNGAEGIGTGWSTFIPNYNPREIVANVRRLLNGESMVPMDPWYRGFKGTIEKTASKEGGCTYTITGLYEEVDETTIRITELPIRRWNDDYKNFLQSLKTDNGAPFFQDVKAYNDEKSVDFDLILSEENMLAARQEGFLKKFKLTTTIATSNMHLFDKKGVIKKYVTPEQILEEFFDLRFEYYEKRKETVVKNMEIELLKLENKARFILAVLSGEIIVNKRKKADIVEDLRQKGFTPFPRKAESVEAAIAGAVDDDAAEEPEEILVDPESSSSYIPGSEYDYLLAMAIASLTIEKVEELLADRDKMIIAVADMKKTTPKSLWLSDLESLDKELEKLDLKDAQVQQAIEAAQKKIRAKSGAAVKVKRQAPKKPAPKKTTKKASESETTEASYSAMDTDNNVAEVVKPKARQGAKKKASESETTEASHSAMDTDNNVAEVVKPKGRQGAKKKAPAAAKEVEEDEMLDLAQRLAQYNFGSAPADSSKTAETSKAIAVDDDDDDVVVEVAPVKKGGRKPAATKAAKPPAAPRKRGKQTVASTEVLAIGVSPEKKVRKMRSSPFNKKSSSVMSRLADNKEEESSENVAGNSSSEKSGGDVSAISRPQRANRRKMTYVLSDSESESANDSEFDDIEDDEDDE

Target ORF information:

RefSeq Version	NM_113294.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana topoisomerase II (TOPII), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_113294.3

ORF Insert Sequence:

ATGGCTACCA AACTTCCCCT TCAAAACAGC AATGCCGCCA ATGTTGCGAA AGCTCCGGCT 
AAATCTCGAG CTGCCGCCGG CGGGAAGACG ATCGAGGAAA TGTATCAGAA GAAATCGCAG 
CTTGAACACA TTCTTCTCCG TCCTGATACA TACATCGGAT CTATCGAGAA ACACACCCAA 
ACACTTTGGG TTTATGAGAA AGATGAGATG GTTCAACGTC CTGTTACCTA TGTACCTGGA 
TTGTATAAGA TCTTTGATGA GATTCTCGTA AACGCCGCTG ATAACAAACA GAGAGATGCG 
AAGATGGATT CCGTTCAAGT TGTGATTGAT GTTGAGCAGA ATCTGATTAG TGTTTGTAAC 
AGTGGAGCTG GAGTTCCTGT AGAGATTCAT CAGGAAGAAG GAATTTATGT ACCGGAGATG 
ATATTTGGTC ATTTGTTGAC GAGTAGTAAC TACGATGATA ATGTGAAGAA GACCACTGGT 
GGAAGAAACG GATATGGTGC TAAGCTTACT AATATCTTCT CTACTGAATT CATCATCGAG 
ACTGCAGATG GGAAGAGACT CAAGAAGTAT AAGCAGGTCT TTGAGAACAA CATGGGGAAG 
AAATCTGAAC CGGTTATAAC CAAGTGTAAT AAGAGTGAGA ACTGGACGAA GGTTACATTC 
AAACCAGATT TGAAGAAGTT TAACATGACT GAATTAGAAG ACGATGTGGT TGCTCTAATG 
AGTAAGCGAG TGTTTGATAT TGCTGGATGT TTGGGGAAGT CTGTCAAAGT TGAGCTGAAT 
GGGAAACAAA TACCAGTGAA GTCATTCACT GATTATGTTG ACCTATACCT AAGTGCAGCT 
AATAAATCGA GGACGGAGGA TCCTCTCCCA AGGTTGACTG AGAAAGTTAA TGATAGATGG 
GAGGTTTGTG TGAGCTTAAG TGAAGGACAA TTTCAGCAGG TCAGCTTTGT CAACTCCATA 
GCAACAATCA AGGGTGGTAC ACATGTTGAC TATGTGACGA GTCAGATTAC AAACCATATT 
GTGGCTGCTG TGAACAAAAA GAATAAAAAC GCAAATGTTA AGGCCCATAA TGTGAAAAAC 
CATCTGTGGG TCTTTGTCAA TGCATTGATT GACAATCCTG CTTTTGATTC TCAAACAAAA 
GAAACACTGA CTCTTCGACA GAGCAGTTTT GGGTCGAAAT GTGAACTTTC AGAAGATTTT 
CTGAAGAAAG TTGGAAAATC TGGCGTAGTG GAGAATTTGC TTTCATGGGC AGATTTCAAG 
CAGAACAAAG ATCTAAAGAA AAGTGATGGA GCCAAAACAG GGAGGGTTCT AGTTGAGAAG 
TTAGACGATG CCGCCGAAGC TGGAGGGAAG AACTCTCGAC TCTGTACGTT AATTTTGACT 
GAAGGAGACT CAGCAAAATC TCTCGCTTTG GCGGGGAGGT CTGTCTTGGG TAATAACTAC 
TGCGGTGTGT TTCCGCTTCG AGGAAAGCTA TTGAATGTCA GGGAAGCCAG CACCACTCAG 
ATTACAAACA ATAAGGAAAT TGAGAACCTC AAGAAGATCC TTGGACTAAA GCAAAATATG 
AAGTACGAGA ATGTTAACTC ATTAAGATAT GGCCAGATGA TGATCATGAC TGATCAAGAT 
CATGATGGTT CTCATATAAA GGGTCTCTTA ATCAATTTTA TCCACTCTTT TTGGCCGTCG 
TTACTCCAGG TTCCATCATT CCTGGTTGAG TTTATAACAC CCATTGTGAA GGCTACGCGT 
AAGGGTACAA AAAAAGTTCT GTCATTCTAC TCAATGCCTG AATACGAAGA ATGGAAAGAA 
AGTCTGAAGG GTAATGCAAC CGGGTGGGAT ATCAAATACT ACAAGGGGTT GGGTACAAGT 
ACGGCTGAGG AGGGCAAAGA GTACTTTAGT AATCTTGGTC TTCACAAAAA GGATTTTGTT 
TGGGAGGATG AACAAGATGG AGAAGCCATT GAACTTGCTT TCAGTAAAAA GAAGATCGAA 
GCCCGGAAAA ATTGGCTTTC GAGCTATGTG CCTGGCAATC ATCTTGATCA GAGACAACCG 
AAAGTTACGT ACAGTGACTT CGTGAACAAA GAACTGATCT TGTTCTCCAT GGCGGATCTT 
CAACGGTCAA TTCCCTCAAT GGTTGATGGG CTCAAACCGG GTCAGAGAAA GATACTTTTC 
GTTGCATTCA AGAAAATCGC TCGTAAAGAA ATGAAGGTTG CCCAGCTCGT TGGTTATGTA 
TCATTGCTGT CAGCTTATCA TCATGGCGAG CAGAGTCTTG CCAGTGCTAT CATAGGCATG 
GCGCAGGATT ACGTGGGCAG CAACAATATC AACTTACTTC TACCAAATGG TCAATTTGGT 
ACACGAACTT CGGGTGGAAA AGATTCAGCT AGTGCAAGAT ACATTTTCAC TAAGCTCTCT 
CCAGTAACAA GGATCTTGTT TCCCAAGGAT GATGATCTCC TTCTTGATTA CTTGAATGAA 
GACGGGCAAA GGATAGAGCC AACTTGGTAC ATGCCCATCA TTCCAACTGT ACTTGTCAAT 
GGCGCTGAAG GCATTGGAAC TGGGTGGAGC ACGTTCATAC CAAACTATAA CCCTAGAGAG 
ATCGTTGCTA ATGTAAGGCG TTTACTTAAC GGTGAAAGTA TGGTGCCTAT GGATCCTTGG 
TATAGAGGTT TTAAAGGAAC AATTGAGAAA ACTGCGTCCA AAGAAGGTGG TTGTACTTAC 
ACAATCACTG GTCTATATGA GGAGGTGGAT GAAACAACTA TTCGAATCAC GGAGTTGCCA 
ATCAGAAGGT GGAATGATGA TTACAAAAAT TTCTTGCAGT CATTGAAGAC AGATAATGGT 
GCACCTTTTT TCCAGGATGT CAAGGCTTAC AATGACGAGA AATCTGTTGA TTTTGACCTT 
ATTTTGAGTG AAGAGAACAT GCTGGCGGCT CGGCAAGAGG GCTTTCTGAA GAAATTCAAA 
CTCACCACGA CAATCGCTAC AAGTAATATG CATCTCTTTG ATAAAAAAGG TGTGATAAAG 
AAATATGTGA CCCCAGAACA AATCCTTGAA GAGTTCTTTG ACCTCAGGTT TGAATACTAT 
GAGAAGAGAA AGGAAACCGT GGTGAAGAAT ATGGAAATTG AGCTGTTGAA ACTAGAAAAC 
AAAGCGAGGT TTATTCTTGC AGTTCTCAGT GGAGAAATCA TAGTGAACAA GAGGAAGAAA 
GCCGATATTG TTGAGGACTT AAGACAGAAA GGGTTCACAC CATTTCCGAG GAAGGCCGAG 
TCTGTCGAAG CTGCAATTGC TGGAGCAGTT GATGATGATG CAGCTGAAGA ACCTGAAGAA 
ATCTTAGTGG ATCCGGAGTC AAGCTCCAGT TATATACCCG GCTCGGAATA TGACTACCTA 
TTGGCAATGG CTATAGCTTC GTTGACTATA GAGAAAGTTG AGGAGTTATT AGCAGACAGA 
GATAAAATGA TTATTGCAGT TGCAGACATG AAGAAAACCA CACCGAAATC CCTCTGGCTC 
AGTGACCTAG AGTCTCTTGA CAAGGAACTA GAAAAACTTG ATCTGAAAGA TGCTCAAGTC 
CAACAGGCTA TAGAGGCAGC ACAGAAAAAA ATCAGGGCCA AATCTGGAGC AGCAGTAAAG 
GTTAAAAGGC AGGCACCAAA GAAACCAGCA CCAAAGAAGA CCACAAAGAA AGCCAGTGAA 
TCTGAGACAA CAGAGGCTTC CTATTCAGCA ATGGACACAG ATAACAACGT AGCAGAAGTG 
GTGAAGCCAA AAGCTAGACA AGGAGCAAAG AAGAAAGCCA GTGAATCTGA AACAACAGAG 
GCTTCCCATT CAGCAATGGA CACAGATAAC AACGTAGCAG AAGTGGTGAA GCCAAAAGGT 
AGACAAGGAG CAAAGAAGAA GGCTCCAGCA GCTGCGAAGG AGGTTGAGGA GGATGAAATG 
TTGGATCTAG CACAACGGCT TGCTCAATAC AATTTCGGCT CAGCACCTGC GGATTCAAGC 
AAAACAGCTG AAACATCGAA AGCAATTGCT GTGGATGACG ATGATGATGA CGTGGTAGTT 
GAGGTTGCAC CAGTAAAGAA AGGAGGAAGG AAACCGGCGG CGACTAAAGC TGCAAAACCA 
CCAGCAGCTC CGAGGAAACG AGGGAAGCAG ACAGTTGCTT CAACAGAGGT TCTGGCAATT 
GGTGTGTCCC CGGAGAAGAA AGTGAGGAAG ATGAGATCAT CTCCGTTCAA CAAGAAGAGT 
AGTTCAGTGA TGAGCAGGTT GGCTGATAAT AAGGAAGAGG AGAGCAGCGA GAATGTGGCT 
GGAAACTCAT CGTCTGAGAA ATCTGGAGGA GATGTTTCTG CGATATCGAG GCCTCAAAGA 
GCTAATAGGA GGAAAATGAC CTATGTGTTG AGTGATTCCG AGAGTGAATC TGCGAATGAT 
TCTGAGTTTG ATGACATTGA AGACGATGAA GATGATGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Nature408(6814)820-2(2000 Dec)
Salanoubat M,Lemcke K,Rieger M,Ansorge W,Unseld M,Fartmann B,Valle G,Bl?cker H,Perez-Alonso M,Obermaier B,Delseny M,Boutry M,Grivell LA,Mache R,Puigdom?nech P,De Simone V,Choisne N,Artiguenave F,Robert C,Brottier P,Wincker P,Cattolico L,Weissenbach J,Saurin W,Qu?tier F,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Benes V,Wurmbach E,Drzonek H,Erfle H,Jordan N,Bangert S,Wiedelmann R,Kranz H,Voss H,Holland R,Brandt P,Nyakatura G,Vezzi A,D'Angelo M,Pallavicini A,Toppo S,Simionati B,Conrad A,Hornischer K,Kauer G,L?hnert TH,Nordsiek G,Reichelt J,Scharfe M,Sch?n O,Bargues M,Terol J,Climent J,Navarro P,Collado C,Perez-Perez A,Ottenw?lder B,Duchemin D,Cooke R,Laudie M,Berger-Llauro C,Purnelle B,Masuy D,de Haan M,Maarse AC,Alcaraz JP,Cottet A,Casacuberta E,Monfort A,Argiriou A,flores M,Liguori R,Vitale D,Mannhaupt G,Haase D,Schoof H,Rudd S,Zaccaria P,Mewes HW,Mayer KF,Kaul S,Town CD,Koo HL,Tallon LJ,Jenkins J,Rooney T,Rizzo M,Walts A,Utterback T,Fujii CY,Shea TP,Creasy TH,Haas B,Maiti R,Wu D,Peterson J,Van Aken S,Pai G,Militscher J,Sellers P,Gill JE,Feldblyum TV,Preuss D,Lin X,Nierman WC,Salzberg SL,White O,Venter JC,Fraser CM,Kaneko T,Nakamura Y,Sato S,Kato T,Asamizu E,Sasamoto S,Kimura T,Idesawa K,Kawashima K,Kishida Y,Kiyokawa C,Kohara M,Matsumoto M,Matsuno A,Muraki A,Nakayama S,Nakazaki N,Shinpo S,Takeuchi C,Wada T,Watanabe A,Yamada M,Yasuda M,Tabata S,,,

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TOPII cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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