Otof cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Otof
Entrez Gene ID	84573
Full Name	otoferlin
General protein information	Preferred Names otoferlin Names otoferlin fer-1-like protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	human homolog is associated with an autosomal recessive form of prelingual sensorineural deafness, DFNB9 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017594387.1	XP_017449876.1	otoferlin isoform X3
XM_008764535.1	XP_008762757.1	otoferlin isoform X1
XM_017594388.1	XP_017449877.1	otoferlin isoform X4
NM_001276720.1	NP_001263649.1	otoferlin
XM_006239833.2	XP_006239895.1	otoferlin isoform X5
XM_008764534.2	XP_008762756.1	otoferlin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	OTOF	NP_919224.1
Canis lupus familiaris (dog)	OTOF	XP_005630285.1
Bos taurus (cattle)	OTOF	NP_001137579.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000389	XP_310715.5
Xenopus tropicalis (tropical clawed frog)	otof	XP_004918245.1
Mus musculus (house mouse)	Otof	NP_114081.2
Gallus gallus (chicken)	OTOF	XP_420015.2
Danio rerio (zebrafish)	otof	NP_001025283.1
Pan troglodytes (chimpanzee)	OTOF	XP_003949828.1
	OTOF	XP_002799220.1
Rattus norvegicus (Norway rat)	Otof	NP_001263649.1

Gene Ontology
Bibliography

Related articles in PubMed

Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.
Wu W, Rahman MN, Guo J, Roy N, Xue L, Cahill CM, Zhang S, Jia Z
Journal of molecular cell biology7(2)168-79(2015 Apr)

Unconventional molecular regulation of synaptic vesicle replenishment in cochlear inner hair cells.
Vogl C, Cooper BH, Neef J, Wojcik SM, Reim K, Reisinger E, Brose N, Rhee JS, Moser T, Wichmann C
Journal of cell science128(4)638-44(2015 Feb)

Calcium regulates molecular interactions of otoferlin with soluble NSF attachment protein receptor (SNARE) proteins required for hair cell exocytosis.
Ramakrishnan NA, Drescher MJ, Morley BJ, Kelley PM, Drescher DG
The Journal of biological chemistry289(13)8750-66(2014 Mar)

Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.
Duncker SV, Franz C, Kuhn S, Schulte U, Campanelli D, Brandt N, Hirt B, Fakler B, Blin N, Ruth P, Engel J, Marcotti W, Zimmermann U, Knipper M
The Journal of neuroscience : the official journal of the Society for Neuroscience33(22)9508-19(2013 May)

Screening of OTOF mutations in : a novel mutation and review.
Mahdieh N, Shirkavand A, Rabbani B, Tekin M, Akbari B, Akbari MT, Zeinali S
International journal of pediatric otorhinolaryngology76(11)1610-5(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

otoferlin, through GAD65, modulates GABAergic activity.
Title: Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.

a direct role for otoferlin in exocytosis and modulation of calcium-dependent membrane fusion
Title: Calcium regulates molecular interactions of otoferlin with soluble NSF attachment protein receptor (SNARE) proteins required for hair cell exocytosis.

otoferlin-AP-2 interaction drives Ca(2+)- and stimulus-dependent compensating clathrin-mediated endocytosis in mature inner hair
Title: Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.

Otof CA domain differs structurally and functionally from other C(2) domains
Title: The crystal structure of the C?A domain of otoferlin reveals an unconventional top loop region.

Using a rodent cochlear cDNA library Rab8b is identified as an interacting partner of otoferlin.
Title: Rab8b GTPase, a protein transport regulator, is an interacting partner of otoferlin, defective in a human autosomal recessive deafness form.

The following Otof gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Otof cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa61776	XM_017594387.1 Latest version!	Rattus norvegicus otoferlin (Otof), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa61777	XM_017594388.1 Latest version!	Rattus norvegicus otoferlin (Otof), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa11529	NM_001276720.1 Latest version!	Rattus norvegicus otoferlin (Otof), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa29177	XM_008764534.2 Latest version!	Rattus norvegicus otoferlin (Otof), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa29178	XM_006239833.2 Latest version!	Rattus norvegicus otoferlin (Otof), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa43851	XM_008764535.1 Latest version!	Rattus norvegicus otoferlin (Otof), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Otof ( XM_017594387.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449876.1 Rattus norvegicus otoferlin (Otof), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa61776
Clone ID Related Accession (Same CDS sequence)	XM_017594387.1
Accession Version	XM_017594387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAACCGA 
CCCGACACGG CGTTCGTCTG GTTCCTGAAC CCACTCAAAT CTATCAAGTA CCTCATTTGC 
ACCCGGTACA AGTGGCTTAT CATCAAGATT GTGCTGGCCC TGCTTGGGCT GCTCATGCTG 
GCCCTCTTCC TCTATAGCCT CCCAGGCTAC ATGGTCAAGA AGCTCCTAGG GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449876.1
CDS	173..6109
Translation	MALIVHLKTVSELRGRADRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVEVSDTLIDDNNAIIKTSLSMEVRYQAADGTVGPWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRAAKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_017594387.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus otoferlin (Otof), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594387.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAACCGA 
CCCGACACGG CGTTCGTCTG GTTCCTGAAC CCACTCAAAT CTATCAAGTA CCTCATTTGC 
ACCCGGTACA AGTGGCTTAT CATCAAGATT GTGCTGGCCC TGCTTGGGCT GCTCATGCTG 
GCCCTCTTCC TCTATAGCCT CCCAGGCTAC ATGGTCAAGA AGCTCCTAGG GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_017594388.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449877.1 Rattus norvegicus otoferlin (Otof), transcript variant X4, mRNA.

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CloneID	ORa61777
Clone ID Related Accession (Same CDS sequence)	XM_017594388.1
Accession Version	XM_017594388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	otoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACTCG GCAAGACTCG GTCCCACAAA GAGGAGCCCC AAAGACAAGA TGAGCCAGCG 
GTGCTGGAGA TGGAGGACCT GGACCACCTA GCCATCCGGC TGGGGGATGG GCTGGATCCT 
GACTCAGTGT CTCTAGCCTC GGTCACTGCT CTCACCAGCA ATGTCTCCAA CAAACGGTCT 
AAGCCAGATA TTAAAATGGA ACCGAGTGCT GGGAGACCCA TGGATTACCA GGTCAGCATC 
ACAGTGATTG AGGCTCGGCA GCTGGTGGGT TTGAACATGG ACCCCGTGGT GTGCGTGGAG 
GTGGGCGATG ACAAAAAATA CACATCCATG AAGGAGTCCA CAAACTGCCC TTACTACAAC 
GAGTACTTCG TCTTTGACTT CCACGTCTCT CCTGATGTCA TGTTTGACAA GATCATCAAG 
ATCTCGGTTA TCCATTCTAA GAACCTGCTT CGAAGCGGCA CTCTGGTGGG CTCCTTCAAA 
ATGGATGTGG GGACCGTGTA CTCCCAGCCT GAACACCAGT TCCATCACAA ATGGGCTATC 
CTGTCAGACC CCGATGACAT CTCTGCCGGG CTGAAGGGCT ATGTAAAGTG TGATGTCGCC 
GTGGTGGGCA AGGGAGACAA CATCAAGACA CCCCACAAGG CCAACGAGAC AGATGAGGAT 
GACATTGAAG GGAACTTGCT GCTCCCCGAG GGCGTGCCCC CAGAACGGCA GTGGGCCCGG 
TTCTACGTGA AAATTTACCG AGCAGAGGGA CTGCCCCGGA TGAACACAAG CCTCATGGCC 
AACGTAAAGA AGGCGTTCAT CGGTGAGAAC AAGGACCTCG TCGACCCCTA CGTTCAAGTC 
TTCTTTGCTG GACAGAAGGG CAAAACATCA GTGCAGAAGA GCAGCTATGA GCCGCTGTGG 
AATGAGCAGG TCGTCTTTAC AGATCTGTTC CCCCCACTCT GCAAGCGCAT GAAGGTGCAG 
ATCCGAGACT CCGACAAGGT CAACGACGTG GCCATCGGCA CCCACTTCAT CGACCTGCGC 
AAGATTTCCA ACGATGGAGA CAAAGGCTTC CTGCCTACGC TCGGCCCAGC CTGGGTGAAC 
ATGTACGGCT CCACACGCAA CTACACGCTG CTGGACGAGC ACCAGGACCT GAATGAGGGC 
CTGGGTGAGG GGGTGTCCTT CCGTGCCCGC CTCATGTTGG GACTGGCTGT GGAGATCCTA 
GACACCTCCA ACCCAGAGCT CACCAGCTCC ACCGAGGTGC AGGTGGAGCA GGCCACACCT 
GTCTCAGAGA GCTGCACAGG GAGAATGGAA GAATTTTTCC TATTTGGAGC CTTCCTGGAA 
GCCTCAATGA TCGACCGGAA AAATGGGGAC AAGCCAGTGA CCTTTGAGGT GACCATAGGA 
AACTATGGCA ATGAAGTCGA TGGTACATCC CGGCCCCAGA GGCCTCGGCC CCGGAAGGAA 
CCTGGGGATG AAGAAGAGGT AGACCTGATT CAGAACTCCA GTGACGACGA GGGTGATGAA 
GCCGGGGACC TGGCCTCAGT GTCCTCCACC CCACCTATGA GGCCCCAGAT CACAGACAGG 
AACTATTTCC ACCTGCCCTA CCTAGAGCGC AAGCCCTGCA TCTACATCAA GAGCTGGTGG 
CCTGACCAGC GGCGCCGTCT CTACAACGCA AACATCATGG ACCACATTGC TGACAAGCTG 
GAAGAAGGTC TGAATGATGT CCAGGAGATG ATCAAAACGG AGAAGTCCTA CCCGGAGCGA 
CGCCTGCGGG GTGTGCTAGA GGAACTCAGC TGTGGCTGCC ACCGCTTCCT CTCCCTCTCG 
GACAAGGACC AGGGTCACTC GTCCCGCACC AGGCTGGATC GGGAGCGCCT CAAATCCTGT 
ATGAGGGAGC TGGAGAGCAT GGGACAACAA GCCAAGAGCC TGAGGGCTCA GGTGAAGCGA 
CACACTGTAC GGGACAAGCT GAGGTTATGC CAGAACTTCC TGCAGAAGCT ACGCTTCCTA 
GCTGATGAGC CCCAGCACAG CATCCCCGAT GTGTTCATTT GGATGATGAG TAATAACAAA 
CGTATCGCCT ACGCCCGCGT GCCTTCCAAA GACCTTTTGT TCTCCATTGT GGAGGAGGAA 
TTGGGCAAGG ACTGCGCCAA GGTCAAGACC CTCTTCCTGA AGCTGCCAGG AAAGAGGGGC 
TTCGGCTCGG CAGGCTGGAC AGTACAGGCC AAGCTGGAAC TCTACCTGTG GCTGGGCCTC 
AGCAAGCAGA GAAAGGACTT CCTGTGTGGC CTGCCTTGTG GCTTCGAGGA GGTCAAGGCA 
GCCCAAGGCC TGGGTCTGCA TTCCTTTCCG CCCATCAGCC TTGTCTACAC CAAGAAGCAA 
ACCTTCCAGC TCCGAGCCCA CATGTATCAG GCCCGAAGCC TCTTCGCCGC TGACAGCACT 
GGGCTCTCTG ATCCTTTTGC CCGAGTCTTC TTCATCAACC AGAGCCAGTG CACGGAGGTC 
CTAAACGAGA CACTGTGTCC TACTTGGGAC CAAATGCTGG TATTTGACAA CCTGGAGCTG 
TACGGTGAAG CTCATGAGTT ACGAGATGAT CCCCCCATCA TTGTCATTGA AATCTACGAC 
CAGGACAGCA TGGGCAAAGC TGACTTCATG GGCCGGACCT TTGCCAAGCC CCTGGTGAAG 
ATGGCAGACG AGGCATATTG CCCGCCTCGC TTCCCGCCCC AGCTTGAGTA CTACCAGATC 
TACCGAGGCA ACGCCACGGC CGGAGACCTA CTGGCTGCCT TTGAGCTGCT GCAGATTGGA 
CCATCGGGGA AGGCTGACCT GCCACCCATC AATGGCCCAG TGGACATGGA CAGAGGGCCC 
ATCATGCCTG TGCCTGTGGG AATCCGGCCC GTGCTCAGCA AGTACAGAGT GGAGGTGCTG 
TTCTGGGGCC TGAGGGACTT AAAGAGGGTG AACCTGGCCC AGGTGGACCG GCCACGGGTG 
GACATCGAGT GTGCGGGAAA AGGGGTACAA TCGTCCCTGA TCCACAATTA TAAGAAGAAC 
CCCAACTTCA ACACCCTGGT CAAGTGGTTT GAAGTGGACC TCCCAGAGAA TGAGCTCCTG 
CACCCACCCT TGAACATCCG GGTGGTGGAC TGCCGGGCCT TTGGACGATA CACCCTGGTG 
GGCTCCCATG CAGTCAGCTC ACTACGACGC TTCATCTACC GACCCCCGGA CCGCTCGGCC 
GCCAACTGGA ACACCACAGT CAGGCTTCTC CGGGGCTACC ACATGCTGTG CAATGGGGGC 
CCCTCTTCTT GCCCCACAGG GGAGGTTGTA GTGAGCATGG AACCTGAAGT GCCAGTTAAG 
AAGCTGGAGA CCCTGGTGAA GCTGGATGCG ACTTCTGATG CTGTGGTCAA GGTGGATGTG 
GCTGAAGATG AGAAGGAAAG GAAGAAGAAG AAAAAGAAAG GCCCATCAGA GGAGGCAGAG 
GAGGAGGAAC CCGATGAGAG CATGCTGGAT TGGTGGTCCA AGTACTTTGC CTCCATTGAC 
ACAATGAAGG AGCAACTTCG ACAACATGAG ACCTCTGGGA TTGATTTGGA AGAGAAGGAA 
GAAATGGATA GCACTGAGGG CCTGAAGGGG CCAGTGAAGA ACAAGGAGAA GTCCAGAGCT 
GCAAAGGAGG AAAAAAAGAA GAAAAATCAG AACCCTGGCC CTGGCCAGGG ATCGGAGGCT 
CCTGAGAAGA AGAAAGCCAA GATCGACGAG CTTAAGGTGT ATCCCAAGGA GCTAGAGTCG 
GAGTTTGACA ACTTTGAGGA CTGGCTGCAC ACGTTCAACC TGTTACGGGG CAAGACTGGG 
GATGATGAGG ATGGCTCCAC AGAGGAAGAA CGCATAGTAG GCCGATTCAA GGGTTCCCTC 
TGTGTGTACA AAGTGCCACT CCCGGAGGAT GTGTCTCGTG AAGCTGGCTA CGATCCCACC 
TACGGAATGT TCCAGGGCAT CCCAAGCAAT GACCCCATTA ATGTGCTGGT CCGAATCTAT 
GTGGTCCGGG CCACAGACCT GCACCCGGCT GACATCAATG GCAAAGCCGA CCCCTATATC 
GCCATCAAGT TAGGCAAGAC TGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC 
AACCCTGTCT TTGGAAAGTC CTTTGACATT GAGGCCTCCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCTG TGTACGACTG GGATCTGGTG GGCACTGATG ACCTCATCGG AGAAACCAAG 
ATCGACCTGG AAAACCGCTT CTACAGCAAG CACCGTGCTA CCTGCGGCAT CGCGCAGACC 
TATTCCATAC ATGGCTATAA TATCTGGAGA GACCCCATGA AGCCCAGCCA GATCCTGACC 
CGCCTCTGCA AAGAGGGCAA AGTGGACGGC CCCCACTTTG GTCCCCATGG GAGAGTGAAG 
GTCGCCAACC GTGTCTTCAC AGGGCCTTCA GAGATAGAGG ATGAGAATGG TCAGAGGAAG 
CCCACAGATG AGCACGTGGC ACTGTCTGCT CTGAGACACT GGGAGGACAT CCCCCGGGTG 
GGCTGCCGCC TTGTACCGGA GCACGTGGAG ACCAGGCCGT TGCTCAACCC TGACAAGCCA 
GGCATTGAGC AGGGCCGCCT GGAGCTGTGG GTGGATATGT TCCCCATGGA TATGCCGGCC 
CCTGGAACAC CTCTGGATAT CTCCCCCAGG AAACCCAAGA AGTATGAGCT GCGCGTCATC 
GTGTGGAACA CAGATGAGGT GGTCCTGGAA GACGACGATT TCTTCACGGG AGAGAAGTCC 
AGTGATATTT TTGTGAGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAACA GGACACAGAC 
GTACATTATC ACTCCCTCAC GGGTGAGGGC AACTTCAACT GGCGATACCT GTTCCCCTTC 
GACTACCTGG CGGCCGAAGA GAAGATCGTT ATGTCCAAGA AGGAGTCCAT GTTCTCCTGG 
GATGAGACGG AGTACAAGAT ACCTGCGCGG CTCACCCTGC AGATCTGGGA CGCTGACCAC 
TTCTCAGCCG ATGACTTCCT GGGGGCCATC GAGCTGGACC TGAACCGGTT CCCGAGGGGC 
GCCAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACCG GGGAGGTGGA TGTGCCCCTG 
GTTTCCATCT TTAAACAGAA ACGTGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG 
AATGATGAAT TTGAACTCAC GGGCAAAGTA GAGGCAGAGT TACACCTCCT CACGGCAGAG 
GAGGCGGAGA AGAACCCGGT GGGCCTGGCT CGCAATGAAC CTGACCCTCT AGAAAAACCC 
AATCGGCCCG ACACAAGCTT CATCTGGTTC TTGAACCCTC TCAAGTCTGC CCGCTACTTC 
CTGTGGCATA CCTACCGCTG GCTGCTCCTC AAACTCCTGC TGCTCCTCCT CCTGCTGCTG 
CTGCTCGCCC TGTTTCTCTA CTCTCTGCCT GGCTACCTGG CCAAGAAGAT CCTTGGGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449877.1
CDS	152..5614
Translation	MKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTVRLLRGYHMLCNGGPSSCPTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRAAKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_017594388.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus otoferlin (Otof), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594388.1

ORF Insert Sequence:

ATGAAACTCG GCAAGACTCG GTCCCACAAA GAGGAGCCCC AAAGACAAGA TGAGCCAGCG 
GTGCTGGAGA TGGAGGACCT GGACCACCTA GCCATCCGGC TGGGGGATGG GCTGGATCCT 
GACTCAGTGT CTCTAGCCTC GGTCACTGCT CTCACCAGCA ATGTCTCCAA CAAACGGTCT 
AAGCCAGATA TTAAAATGGA ACCGAGTGCT GGGAGACCCA TGGATTACCA GGTCAGCATC 
ACAGTGATTG AGGCTCGGCA GCTGGTGGGT TTGAACATGG ACCCCGTGGT GTGCGTGGAG 
GTGGGCGATG ACAAAAAATA CACATCCATG AAGGAGTCCA CAAACTGCCC TTACTACAAC 
GAGTACTTCG TCTTTGACTT CCACGTCTCT CCTGATGTCA TGTTTGACAA GATCATCAAG 
ATCTCGGTTA TCCATTCTAA GAACCTGCTT CGAAGCGGCA CTCTGGTGGG CTCCTTCAAA 
ATGGATGTGG GGACCGTGTA CTCCCAGCCT GAACACCAGT TCCATCACAA ATGGGCTATC 
CTGTCAGACC CCGATGACAT CTCTGCCGGG CTGAAGGGCT ATGTAAAGTG TGATGTCGCC 
GTGGTGGGCA AGGGAGACAA CATCAAGACA CCCCACAAGG CCAACGAGAC AGATGAGGAT 
GACATTGAAG GGAACTTGCT GCTCCCCGAG GGCGTGCCCC CAGAACGGCA GTGGGCCCGG 
TTCTACGTGA AAATTTACCG AGCAGAGGGA CTGCCCCGGA TGAACACAAG CCTCATGGCC 
AACGTAAAGA AGGCGTTCAT CGGTGAGAAC AAGGACCTCG TCGACCCCTA CGTTCAAGTC 
TTCTTTGCTG GACAGAAGGG CAAAACATCA GTGCAGAAGA GCAGCTATGA GCCGCTGTGG 
AATGAGCAGG TCGTCTTTAC AGATCTGTTC CCCCCACTCT GCAAGCGCAT GAAGGTGCAG 
ATCCGAGACT CCGACAAGGT CAACGACGTG GCCATCGGCA CCCACTTCAT CGACCTGCGC 
AAGATTTCCA ACGATGGAGA CAAAGGCTTC CTGCCTACGC TCGGCCCAGC CTGGGTGAAC 
ATGTACGGCT CCACACGCAA CTACACGCTG CTGGACGAGC ACCAGGACCT GAATGAGGGC 
CTGGGTGAGG GGGTGTCCTT CCGTGCCCGC CTCATGTTGG GACTGGCTGT GGAGATCCTA 
GACACCTCCA ACCCAGAGCT CACCAGCTCC ACCGAGGTGC AGGTGGAGCA GGCCACACCT 
GTCTCAGAGA GCTGCACAGG GAGAATGGAA GAATTTTTCC TATTTGGAGC CTTCCTGGAA 
GCCTCAATGA TCGACCGGAA AAATGGGGAC AAGCCAGTGA CCTTTGAGGT GACCATAGGA 
AACTATGGCA ATGAAGTCGA TGGTACATCC CGGCCCCAGA GGCCTCGGCC CCGGAAGGAA 
CCTGGGGATG AAGAAGAGGT AGACCTGATT CAGAACTCCA GTGACGACGA GGGTGATGAA 
GCCGGGGACC TGGCCTCAGT GTCCTCCACC CCACCTATGA GGCCCCAGAT CACAGACAGG 
AACTATTTCC ACCTGCCCTA CCTAGAGCGC AAGCCCTGCA TCTACATCAA GAGCTGGTGG 
CCTGACCAGC GGCGCCGTCT CTACAACGCA AACATCATGG ACCACATTGC TGACAAGCTG 
GAAGAAGGTC TGAATGATGT CCAGGAGATG ATCAAAACGG AGAAGTCCTA CCCGGAGCGA 
CGCCTGCGGG GTGTGCTAGA GGAACTCAGC TGTGGCTGCC ACCGCTTCCT CTCCCTCTCG 
GACAAGGACC AGGGTCACTC GTCCCGCACC AGGCTGGATC GGGAGCGCCT CAAATCCTGT 
ATGAGGGAGC TGGAGAGCAT GGGACAACAA GCCAAGAGCC TGAGGGCTCA GGTGAAGCGA 
CACACTGTAC GGGACAAGCT GAGGTTATGC CAGAACTTCC TGCAGAAGCT ACGCTTCCTA 
GCTGATGAGC CCCAGCACAG CATCCCCGAT GTGTTCATTT GGATGATGAG TAATAACAAA 
CGTATCGCCT ACGCCCGCGT GCCTTCCAAA GACCTTTTGT TCTCCATTGT GGAGGAGGAA 
TTGGGCAAGG ACTGCGCCAA GGTCAAGACC CTCTTCCTGA AGCTGCCAGG AAAGAGGGGC 
TTCGGCTCGG CAGGCTGGAC AGTACAGGCC AAGCTGGAAC TCTACCTGTG GCTGGGCCTC 
AGCAAGCAGA GAAAGGACTT CCTGTGTGGC CTGCCTTGTG GCTTCGAGGA GGTCAAGGCA 
GCCCAAGGCC TGGGTCTGCA TTCCTTTCCG CCCATCAGCC TTGTCTACAC CAAGAAGCAA 
ACCTTCCAGC TCCGAGCCCA CATGTATCAG GCCCGAAGCC TCTTCGCCGC TGACAGCACT 
GGGCTCTCTG ATCCTTTTGC CCGAGTCTTC TTCATCAACC AGAGCCAGTG CACGGAGGTC 
CTAAACGAGA CACTGTGTCC TACTTGGGAC CAAATGCTGG TATTTGACAA CCTGGAGCTG 
TACGGTGAAG CTCATGAGTT ACGAGATGAT CCCCCCATCA TTGTCATTGA AATCTACGAC 
CAGGACAGCA TGGGCAAAGC TGACTTCATG GGCCGGACCT TTGCCAAGCC CCTGGTGAAG 
ATGGCAGACG AGGCATATTG CCCGCCTCGC TTCCCGCCCC AGCTTGAGTA CTACCAGATC 
TACCGAGGCA ACGCCACGGC CGGAGACCTA CTGGCTGCCT TTGAGCTGCT GCAGATTGGA 
CCATCGGGGA AGGCTGACCT GCCACCCATC AATGGCCCAG TGGACATGGA CAGAGGGCCC 
ATCATGCCTG TGCCTGTGGG AATCCGGCCC GTGCTCAGCA AGTACAGAGT GGAGGTGCTG 
TTCTGGGGCC TGAGGGACTT AAAGAGGGTG AACCTGGCCC AGGTGGACCG GCCACGGGTG 
GACATCGAGT GTGCGGGAAA AGGGGTACAA TCGTCCCTGA TCCACAATTA TAAGAAGAAC 
CCCAACTTCA ACACCCTGGT CAAGTGGTTT GAAGTGGACC TCCCAGAGAA TGAGCTCCTG 
CACCCACCCT TGAACATCCG GGTGGTGGAC TGCCGGGCCT TTGGACGATA CACCCTGGTG 
GGCTCCCATG CAGTCAGCTC ACTACGACGC TTCATCTACC GACCCCCGGA CCGCTCGGCC 
GCCAACTGGA ACACCACAGT CAGGCTTCTC CGGGGCTACC ACATGCTGTG CAATGGGGGC 
CCCTCTTCTT GCCCCACAGG GGAGGTTGTA GTGAGCATGG AACCTGAAGT GCCAGTTAAG 
AAGCTGGAGA CCCTGGTGAA GCTGGATGCG ACTTCTGATG CTGTGGTCAA GGTGGATGTG 
GCTGAAGATG AGAAGGAAAG GAAGAAGAAG AAAAAGAAAG GCCCATCAGA GGAGGCAGAG 
GAGGAGGAAC CCGATGAGAG CATGCTGGAT TGGTGGTCCA AGTACTTTGC CTCCATTGAC 
ACAATGAAGG AGCAACTTCG ACAACATGAG ACCTCTGGGA TTGATTTGGA AGAGAAGGAA 
GAAATGGATA GCACTGAGGG CCTGAAGGGG CCAGTGAAGA ACAAGGAGAA GTCCAGAGCT 
GCAAAGGAGG AAAAAAAGAA GAAAAATCAG AACCCTGGCC CTGGCCAGGG ATCGGAGGCT 
CCTGAGAAGA AGAAAGCCAA GATCGACGAG CTTAAGGTGT ATCCCAAGGA GCTAGAGTCG 
GAGTTTGACA ACTTTGAGGA CTGGCTGCAC ACGTTCAACC TGTTACGGGG CAAGACTGGG 
GATGATGAGG ATGGCTCCAC AGAGGAAGAA CGCATAGTAG GCCGATTCAA GGGTTCCCTC 
TGTGTGTACA AAGTGCCACT CCCGGAGGAT GTGTCTCGTG AAGCTGGCTA CGATCCCACC 
TACGGAATGT TCCAGGGCAT CCCAAGCAAT GACCCCATTA ATGTGCTGGT CCGAATCTAT 
GTGGTCCGGG CCACAGACCT GCACCCGGCT GACATCAATG GCAAAGCCGA CCCCTATATC 
GCCATCAAGT TAGGCAAGAC TGACATCCGA GACAAGGAGA ACTACATCTC CAAGCAGCTC 
AACCCTGTCT TTGGAAAGTC CTTTGACATT GAGGCCTCCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCTG TGTACGACTG GGATCTGGTG GGCACTGATG ACCTCATCGG AGAAACCAAG 
ATCGACCTGG AAAACCGCTT CTACAGCAAG CACCGTGCTA CCTGCGGCAT CGCGCAGACC 
TATTCCATAC ATGGCTATAA TATCTGGAGA GACCCCATGA AGCCCAGCCA GATCCTGACC 
CGCCTCTGCA AAGAGGGCAA AGTGGACGGC CCCCACTTTG GTCCCCATGG GAGAGTGAAG 
GTCGCCAACC GTGTCTTCAC AGGGCCTTCA GAGATAGAGG ATGAGAATGG TCAGAGGAAG 
CCCACAGATG AGCACGTGGC ACTGTCTGCT CTGAGACACT GGGAGGACAT CCCCCGGGTG 
GGCTGCCGCC TTGTACCGGA GCACGTGGAG ACCAGGCCGT TGCTCAACCC TGACAAGCCA 
GGCATTGAGC AGGGCCGCCT GGAGCTGTGG GTGGATATGT TCCCCATGGA TATGCCGGCC 
CCTGGAACAC CTCTGGATAT CTCCCCCAGG AAACCCAAGA AGTATGAGCT GCGCGTCATC 
GTGTGGAACA CAGATGAGGT GGTCCTGGAA GACGACGATT TCTTCACGGG AGAGAAGTCC 
AGTGATATTT TTGTGAGGGG GTGGCTGAAG GGCCAGCAGG AGGACAAACA GGACACAGAC 
GTACATTATC ACTCCCTCAC GGGTGAGGGC AACTTCAACT GGCGATACCT GTTCCCCTTC 
GACTACCTGG CGGCCGAAGA GAAGATCGTT ATGTCCAAGA AGGAGTCCAT GTTCTCCTGG 
GATGAGACGG AGTACAAGAT ACCTGCGCGG CTCACCCTGC AGATCTGGGA CGCTGACCAC 
TTCTCAGCCG ATGACTTCCT GGGGGCCATC GAGCTGGACC TGAACCGGTT CCCGAGGGGC 
GCCAAGACAG CCAAGCAGTG CACCATGGAG ATGGCCACCG GGGAGGTGGA TGTGCCCCTG 
GTTTCCATCT TTAAACAGAA ACGTGTCAAA GGCTGGTGGC CCCTCCTGGC CCGCAATGAG 
AATGATGAAT TTGAACTCAC GGGCAAAGTA GAGGCAGAGT TACACCTCCT CACGGCAGAG 
GAGGCGGAGA AGAACCCGGT GGGCCTGGCT CGCAATGAAC CTGACCCTCT AGAAAAACCC 
AATCGGCCCG ACACAAGCTT CATCTGGTTC TTGAACCCTC TCAAGTCTGC CCGCTACTTC 
CTGTGGCATA CCTACCGCTG GCTGCTCCTC AAACTCCTGC TGCTCCTCCT CCTGCTGCTG 
CTGCTCGCCC TGTTTCTCTA CTCTCTGCCT GGCTACCTGG CCAAGAAGAT CCTTGGGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( NM_001276720.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001263649.1 Rattus norvegicus otoferlin (Otof), mRNA.

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CloneID	ORa11529
Clone ID Related Accession (Same CDS sequence)	NM_001276720.1
Accession Version	NM_001276720.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Rattus norvegicus(Norway rat)
Product	otoferlin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07063295.1. On or before Mar 1, 2013 this sequence version replaced XM_002729613.2, XM_001062291.3, XM_003750118.1, XM_001065408.3, XM_002726716.2, XM_003754154.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052297, SAMEA2689595 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGTCAGGCT TCTCCGGGGC TACCACATGC TGTGCAATGG GGGCCCCTCT 
TCTTGCCCCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAATCGG 
CCCGACACAA GCTTCATCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCCTGTGG 
CATACCTACC GCTGGCTGCT CCTCAAACTC CTGCTGCTCC TCCTCCTGCT GCTGCTGCTC 
GCCCTGTTTC TCTACTCTCT GCCTGGCTAC CTGGCCAAGA AGATCCTTGG GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263649.1
CDS	110..6106
Translation	MALIVHLKTVSELRGRADRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVEVSDTLIDDNNAIIKTSLSMEVRYQAADGTVGPWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTVRLLRGYHMLCNGGPSSCPTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRAAKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	NM_001276720.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus otoferlin (Otof), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276720.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGTCAGGCT TCTCCGGGGC TACCACATGC TGTGCAATGG GGGCCCCTCT 
TCTTGCCCCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAATCGG 
CCCGACACAA GCTTCATCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCCTGTGG 
CATACCTACC GCTGGCTGCT CCTCAAACTC CTGCTGCTCC TCCTCCTGCT GCTGCTGCTC 
GCCCTGTTTC TCTACTCTCT GCCTGGCTAC CTGGCCAAGA AGATCCTTGG GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_008764534.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762756.1 Rattus norvegicus otoferlin (Otof), transcript variant X2, mRNA.

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CloneID	ORa29177
Clone ID Related Accession (Same CDS sequence)	XM_008764534.2
Accession Version	XM_008764534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764534.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAATCGG 
CCCGACACAA GCTTCATCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCCTGTGG 
CATACCTACC GCTGGCTGCT CCTCAAACTC CTGCTGCTCC TCCTCCTGCT GCTGCTGCTC 
GCCCTGTTTC TCTACTCTCT GCCTGGCTAC CTGGCCAAGA AGATCCTTGG GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762756.1
CDS	173..6109
Translation	MALIVHLKTVSELRGRADRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVEVSDTLIDDNNAIIKTSLSMEVRYQAADGTVGPWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRAAKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSLPGYLAKKILGA

Target ORF information:

RefSeq Version	XM_008764534.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus otoferlin (Otof), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764534.2

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAATCGG 
CCCGACACAA GCTTCATCTG GTTCTTGAAC CCTCTCAAGT CTGCCCGCTA CTTCCTGTGG 
CATACCTACC GCTGGCTGCT CCTCAAACTC CTGCTGCTCC TCCTCCTGCT GCTGCTGCTC 
GCCCTGTTTC TCTACTCTCT GCCTGGCTAC CTGGCCAAGA AGATCCTTGG GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_006239833.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239895.1 Rattus norvegicus otoferlin (Otof), transcript variant X5, mRNA.

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CloneID	ORa29178
Clone ID Related Accession (Same CDS sequence)	XM_006239833.2
Accession Version	XM_006239833.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	otoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006239833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGC AAAGGCAAAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC ACAAAGCGGG AAGGAGTGTA TTCTCGGCCA TGAAACTCGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCGG TGCTGGAGAT GGAGGACCTG 
GACCACCTAG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCG 
GTCACTGCTC TCACCAGCAA TGTCTCCAAC AAACGGTCTA AGCCAGATAT TAAAATGGAA 
CCGAGTGCTG GGAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATTGA GGCTCGGCAG 
CTGGTGGGTT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGCGATGA CAAAAAATAC 
ACATCCATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTACTTCGT CTTTGACTTC 
CACGTCTCTC CTGATGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTTAT CCATTCTAAG 
AACCTGCTTC GAAGCGGCAC TCTGGTGGGC TCCTTCAAAA TGGATGTGGG GACCGTGTAC 
TCCCAGCCTG AACACCAGTT CCATCACAAA TGGGCTATCC TGTCAGACCC CGATGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCCG TGGTGGGCAA GGGAGACAAC 
ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAAGG GAACTTGCTG 
CTCCCCGAGG GCGTGCCCCC AGAACGGCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCAGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTAAAGAA GGCGTTCATC 
GGTGAGAACA AGGACCTCGT CGACCCCTAC GTTCAAGTCT TCTTTGCTGG ACAGAAGGGC 
AAAACATCAG TGCAGAAGAG CAGCTATGAG CCGCTGTGGA ATGAGCAGGT CGTCTTTACA 
GATCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC CGACAAGGTC 
AACGACGTGG CCATCGGCAC CCACTTCATC GACCTGCGCA AGATTTCCAA CGATGGAGAC 
AAAGGCTTCC TGCCTACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGCAAC 
TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGTGAGGG GGTGTCCTTC 
CGTGCCCGCC TCATGTTGGG ACTGGCTGTG GAGATCCTAG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CCGAGGTGCA GGTGGAGCAG GCCACACCTG TCTCAGAGAG CTGCACAGGG 
AGAATGGAAG AATTTTTCCT ATTTGGAGCC TTCCTGGAAG CCTCAATGAT CGACCGGAAA 
AATGGGGACA AGCCAGTGAC CTTTGAGGTG ACCATAGGAA ACTATGGCAA TGAAGTCGAT 
GGTACATCCC GGCCCCAGAG GCCTCGGCCC CGGAAGGAAC CTGGGGATGA AGAAGAGGTA 
GACCTGATTC AGAACTCCAG TGACGACGAG GGTGATGAAG CCGGGGACCT GGCCTCAGTG 
TCCTCCACCC CACCTATGAG GCCCCAGATC ACAGACAGGA ACTATTTCCA CCTGCCCTAC 
CTAGAGCGCA AGCCCTGCAT CTACATCAAG AGCTGGTGGC CTGACCAGCG GCGCCGTCTC 
TACAACGCAA ACATCATGGA CCACATTGCT GACAAGCTGG AAGAAGGTCT GAATGATGTC 
CAGGAGATGA TCAAAACGGA GAAGTCCTAC CCGGAGCGAC GCCTGCGGGG TGTGCTAGAG 
GAACTCAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGTCACTCG 
TCCCGCACCA GGCTGGATCG GGAGCGCCTC AAATCCTGTA TGAGGGAGCT GGAGAGCATG 
GGACAACAAG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTACG GGACAAGCTG 
AGGTTATGCC AGAACTTCCT GCAGAAGCTA CGCTTCCTAG CTGATGAGCC CCAGCACAGC 
ATCCCCGATG TGTTCATTTG GATGATGAGT AATAACAAAC GTATCGCCTA CGCCCGCGTG 
CCTTCCAAAG ACCTTTTGTT CTCCATTGTG GAGGAGGAAT TGGGCAAGGA CTGCGCCAAG 
GTCAAGACCC TCTTCCTGAA GCTGCCAGGA AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAACT CTACCTGTGG CTGGGCCTCA GCAAGCAGAG AAAGGACTTC 
CTGTGTGGCC TGCCTTGTGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAT 
TCCTTTCCGC CCATCAGCCT TGTCTACACC AAGAAGCAAA CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTCGCCGCT GACAGCACTG GGCTCTCTGA TCCTTTTGCC 
CGAGTCTTCT TCATCAACCA GAGCCAGTGC ACGGAGGTCC TAAACGAGAC ACTGTGTCCT 
ACTTGGGACC AAATGCTGGT ATTTGACAAC CTGGAGCTGT ACGGTGAAGC TCATGAGTTA 
CGAGATGATC CCCCCATCAT TGTCATTGAA ATCTACGACC AGGACAGCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCAGACGA GGCATATTGC 
CCGCCTCGCT TCCCGCCCCA GCTTGAGTAC TACCAGATCT ACCGAGGCAA CGCCACGGCC 
GGAGACCTAC TGGCTGCCTT TGAGCTGCTG CAGATTGGAC CATCGGGGAA GGCTGACCTG 
CCACCCATCA ATGGCCCAGT GGACATGGAC AGAGGGCCCA TCATGCCTGT GCCTGTGGGA 
ATCCGGCCCG TGCTCAGCAA GTACAGAGTG GAGGTGCTGT TCTGGGGCCT GAGGGACTTA 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGG CCACGGGTGG ACATCGAGTG TGCGGGAAAA 
GGGGTACAAT CGTCCCTGAT CCACAATTAT AAGAAGAACC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGG 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC AGTCAGCTCA 
CTACGACGCT TCATCTACCG ACCCCCGGAC CGCTCGGCCG CCAACTGGAA CACCACAGGG 
GAGGTTGTAG TGAGCATGGA ACCTGAAGTG CCAGTTAAGA AGCTGGAGAC CCTGGTGAAG 
CTGGATGCGA CTTCTGATGC TGTGGTCAAG GTGGATGTGG CTGAAGATGA GAAGGAAAGG 
AAGAAGAAGA AAAAGAAAGG CCCATCAGAG GAGGCAGAGG AGGAGGAACC CGATGAGAGC 
ATGCTGGATT GGTGGTCCAA GTACTTTGCC TCCATTGACA CAATGAAGGA GCAACTTCGA 
CAACATGAGA CCTCTGGGAT TGATTTGGAA GAGAAGGAAG AAATGGATAG CACTGAGGGC 
CTGAAGGGGC CAGTGAAGAA CAAGGAGAAG TCCAGAGCTG CAAAGGAGGA AAAAAAGAAG 
AAAAATCAGA ACCCTGGCCC TGGCCAGGGA TCGGAGGCTC CTGAGAAGAA GAAAGCCAAG 
ATCGACGAGC TTAAGGTGTA TCCCAAGGAG CTAGAGTCGG AGTTTGACAA CTTTGAGGAC 
TGGCTGCACA CGTTCAACCT GTTACGGGGC AAGACTGGGG ATGATGAGGA TGGCTCCACA 
GAGGAAGAAC GCATAGTAGG CCGATTCAAG GGTTCCCTCT GTGTGTACAA AGTGCCACTC 
CCGGAGGATG TGTCTCGTGA AGCTGGCTAC GATCCCACCT ACGGAATGTT CCAGGGCATC 
CCAAGCAATG ACCCCATTAA TGTGCTGGTC CGAATCTATG TGGTCCGGGC CACAGACCTG 
CACCCGGCTG ACATCAATGG CAAAGCCGAC CCCTATATCG CCATCAAGTT AGGCAAGACT 
GACATCCGAG ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGAAAGTCC 
TTTGACATTG AGGCCTCCTT CCCCATGGAG TCCATGCTGA CAGTGGCTGT GTACGACTGG 
GATCTGGTGG GCACTGATGA CCTCATCGGA GAAACCAAGA TCGACCTGGA AAACCGCTTC 
TACAGCAAGC ACCGTGCTAC CTGCGGCATC GCGCAGACCT ATTCCATACA TGGCTATAAT 
ATCTGGAGAG ACCCCATGAA GCCCAGCCAG ATCCTGACCC GCCTCTGCAA AGAGGGCAAA 
GTGGACGGCC CCCACTTTGG TCCCCATGGG AGAGTGAAGG TCGCCAACCG TGTCTTCACA 
GGGCCTTCAG AGATAGAGGA TGAGAATGGT CAGAGGAAGC CCACAGATGA GCACGTGGCA 
CTGTCTGCTC TGAGACACTG GGAGGACATC CCCCGGGTGG GCTGCCGCCT TGTACCGGAG 
CACGTGGAGA CCAGGCCGTT GCTCAACCCT GACAAGCCAG GCATTGAGCA GGGCCGCCTG 
GAGCTGTGGG TGGATATGTT CCCCATGGAT ATGCCGGCCC CTGGAACACC TCTGGATATC 
TCCCCCAGGA AACCCAAGAA GTATGAGCTG CGCGTCATCG TGTGGAACAC AGATGAGGTG 
GTCCTGGAAG ACGACGATTT CTTCACGGGA GAGAAGTCCA GTGATATTTT TGTGAGGGGG 
TGGCTGAAGG GCCAGCAGGA GGACAAACAG GACACAGACG TACATTATCA CTCCCTCACG 
GGTGAGGGCA ACTTCAACTG GCGATACCTG TTCCCCTTCG ACTACCTGGC GGCCGAAGAG 
AAGATCGTTA TGTCCAAGAA GGAGTCCATG TTCTCCTGGG ATGAGACGGA GTACAAGATA 
CCTGCGCGGC TCACCCTGCA GATCTGGGAC GCTGACCACT TCTCAGCCGA TGACTTCCTG 
GGGGCCATCG AGCTGGACCT GAACCGGTTC CCGAGGGGCG CCAAGACAGC CAAGCAGTGC 
ACCATGGAGA TGGCCACCGG GGAGGTGGAT GTGCCCCTGG TTTCCATCTT TAAACAGAAA 
CGTGTCAAAG GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ATGATGAATT TGAACTCACG 
GGCAAAGTAG AGGCAGAGTT ACACCTCCTC ACGGCAGAGG AGGCGGAGAA GAACCCGGTG 
GGCCTGGCTC GCAATGAACC TGACCCTCTA GAAAAACCCA ACCGACCCGA CACGGCGTTC 
GTCTGGTTCC TGAACCCACT CAAATCTATC AAGTACCTCA TTTGCACCCG GTACAAGTGG 
CTTATCATCA AGATTGTGCT GGCCCTGCTT GGGCTGCTCA TGCTGGCCCT CTTCCTCTAT 
AGCCTCCCAG GCTACATGGT CAAGAAGCTC CTAGGGGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239895.1
CDS	173..6154
Translation	MALIVHLKTVSELRGRADRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVEVSDTLIDDNNAIIKTSLSMEVRYQAADGTVGPWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSTRTPGEKSFRSKGKEKTKGGRDGEHKAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRAAKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_006239833.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus otoferlin (Otof), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239833.2

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGC AAAGGCAAAG AGAAGACCAA GGGAGGCAGA 
GATGGCGAGC ACAAAGCGGG AAGGAGTGTA TTCTCGGCCA TGAAACTCGG CAAGACTCGG 
TCCCACAAAG AGGAGCCCCA AAGACAAGAT GAGCCAGCGG TGCTGGAGAT GGAGGACCTG 
GACCACCTAG CCATCCGGCT GGGGGATGGG CTGGATCCTG ACTCAGTGTC TCTAGCCTCG 
GTCACTGCTC TCACCAGCAA TGTCTCCAAC AAACGGTCTA AGCCAGATAT TAAAATGGAA 
CCGAGTGCTG GGAGACCCAT GGATTACCAG GTCAGCATCA CAGTGATTGA GGCTCGGCAG 
CTGGTGGGTT TGAACATGGA CCCCGTGGTG TGCGTGGAGG TGGGCGATGA CAAAAAATAC 
ACATCCATGA AGGAGTCCAC AAACTGCCCT TACTACAACG AGTACTTCGT CTTTGACTTC 
CACGTCTCTC CTGATGTCAT GTTTGACAAG ATCATCAAGA TCTCGGTTAT CCATTCTAAG 
AACCTGCTTC GAAGCGGCAC TCTGGTGGGC TCCTTCAAAA TGGATGTGGG GACCGTGTAC 
TCCCAGCCTG AACACCAGTT CCATCACAAA TGGGCTATCC TGTCAGACCC CGATGACATC 
TCTGCCGGGC TGAAGGGCTA TGTAAAGTGT GATGTCGCCG TGGTGGGCAA GGGAGACAAC 
ATCAAGACAC CCCACAAGGC CAACGAGACA GATGAGGATG ACATTGAAGG GAACTTGCTG 
CTCCCCGAGG GCGTGCCCCC AGAACGGCAG TGGGCCCGGT TCTACGTGAA AATTTACCGA 
GCAGAGGGAC TGCCCCGGAT GAACACAAGC CTCATGGCCA ACGTAAAGAA GGCGTTCATC 
GGTGAGAACA AGGACCTCGT CGACCCCTAC GTTCAAGTCT TCTTTGCTGG ACAGAAGGGC 
AAAACATCAG TGCAGAAGAG CAGCTATGAG CCGCTGTGGA ATGAGCAGGT CGTCTTTACA 
GATCTGTTCC CCCCACTCTG CAAGCGCATG AAGGTGCAGA TCCGAGACTC CGACAAGGTC 
AACGACGTGG CCATCGGCAC CCACTTCATC GACCTGCGCA AGATTTCCAA CGATGGAGAC 
AAAGGCTTCC TGCCTACGCT CGGCCCAGCC TGGGTGAACA TGTACGGCTC CACACGCAAC 
TACACGCTGC TGGACGAGCA CCAGGACCTG AATGAGGGCC TGGGTGAGGG GGTGTCCTTC 
CGTGCCCGCC TCATGTTGGG ACTGGCTGTG GAGATCCTAG ACACCTCCAA CCCAGAGCTC 
ACCAGCTCCA CCGAGGTGCA GGTGGAGCAG GCCACACCTG TCTCAGAGAG CTGCACAGGG 
AGAATGGAAG AATTTTTCCT ATTTGGAGCC TTCCTGGAAG CCTCAATGAT CGACCGGAAA 
AATGGGGACA AGCCAGTGAC CTTTGAGGTG ACCATAGGAA ACTATGGCAA TGAAGTCGAT 
GGTACATCCC GGCCCCAGAG GCCTCGGCCC CGGAAGGAAC CTGGGGATGA AGAAGAGGTA 
GACCTGATTC AGAACTCCAG TGACGACGAG GGTGATGAAG CCGGGGACCT GGCCTCAGTG 
TCCTCCACCC CACCTATGAG GCCCCAGATC ACAGACAGGA ACTATTTCCA CCTGCCCTAC 
CTAGAGCGCA AGCCCTGCAT CTACATCAAG AGCTGGTGGC CTGACCAGCG GCGCCGTCTC 
TACAACGCAA ACATCATGGA CCACATTGCT GACAAGCTGG AAGAAGGTCT GAATGATGTC 
CAGGAGATGA TCAAAACGGA GAAGTCCTAC CCGGAGCGAC GCCTGCGGGG TGTGCTAGAG 
GAACTCAGCT GTGGCTGCCA CCGCTTCCTC TCCCTCTCGG ACAAGGACCA GGGTCACTCG 
TCCCGCACCA GGCTGGATCG GGAGCGCCTC AAATCCTGTA TGAGGGAGCT GGAGAGCATG 
GGACAACAAG CCAAGAGCCT GAGGGCTCAG GTGAAGCGAC ACACTGTACG GGACAAGCTG 
AGGTTATGCC AGAACTTCCT GCAGAAGCTA CGCTTCCTAG CTGATGAGCC CCAGCACAGC 
ATCCCCGATG TGTTCATTTG GATGATGAGT AATAACAAAC GTATCGCCTA CGCCCGCGTG 
CCTTCCAAAG ACCTTTTGTT CTCCATTGTG GAGGAGGAAT TGGGCAAGGA CTGCGCCAAG 
GTCAAGACCC TCTTCCTGAA GCTGCCAGGA AAGAGGGGCT TCGGCTCGGC AGGCTGGACA 
GTACAGGCCA AGCTGGAACT CTACCTGTGG CTGGGCCTCA GCAAGCAGAG AAAGGACTTC 
CTGTGTGGCC TGCCTTGTGG CTTCGAGGAG GTCAAGGCAG CCCAAGGCCT GGGTCTGCAT 
TCCTTTCCGC CCATCAGCCT TGTCTACACC AAGAAGCAAA CCTTCCAGCT CCGAGCCCAC 
ATGTATCAGG CCCGAAGCCT CTTCGCCGCT GACAGCACTG GGCTCTCTGA TCCTTTTGCC 
CGAGTCTTCT TCATCAACCA GAGCCAGTGC ACGGAGGTCC TAAACGAGAC ACTGTGTCCT 
ACTTGGGACC AAATGCTGGT ATTTGACAAC CTGGAGCTGT ACGGTGAAGC TCATGAGTTA 
CGAGATGATC CCCCCATCAT TGTCATTGAA ATCTACGACC AGGACAGCAT GGGCAAAGCT 
GACTTCATGG GCCGGACCTT TGCCAAGCCC CTGGTGAAGA TGGCAGACGA GGCATATTGC 
CCGCCTCGCT TCCCGCCCCA GCTTGAGTAC TACCAGATCT ACCGAGGCAA CGCCACGGCC 
GGAGACCTAC TGGCTGCCTT TGAGCTGCTG CAGATTGGAC CATCGGGGAA GGCTGACCTG 
CCACCCATCA ATGGCCCAGT GGACATGGAC AGAGGGCCCA TCATGCCTGT GCCTGTGGGA 
ATCCGGCCCG TGCTCAGCAA GTACAGAGTG GAGGTGCTGT TCTGGGGCCT GAGGGACTTA 
AAGAGGGTGA ACCTGGCCCA GGTGGACCGG CCACGGGTGG ACATCGAGTG TGCGGGAAAA 
GGGGTACAAT CGTCCCTGAT CCACAATTAT AAGAAGAACC CCAACTTCAA CACCCTGGTC 
AAGTGGTTTG AAGTGGACCT CCCAGAGAAT GAGCTCCTGC ACCCACCCTT GAACATCCGG 
GTGGTGGACT GCCGGGCCTT TGGACGATAC ACCCTGGTGG GCTCCCATGC AGTCAGCTCA 
CTACGACGCT TCATCTACCG ACCCCCGGAC CGCTCGGCCG CCAACTGGAA CACCACAGGG 
GAGGTTGTAG TGAGCATGGA ACCTGAAGTG CCAGTTAAGA AGCTGGAGAC CCTGGTGAAG 
CTGGATGCGA CTTCTGATGC TGTGGTCAAG GTGGATGTGG CTGAAGATGA GAAGGAAAGG 
AAGAAGAAGA AAAAGAAAGG CCCATCAGAG GAGGCAGAGG AGGAGGAACC CGATGAGAGC 
ATGCTGGATT GGTGGTCCAA GTACTTTGCC TCCATTGACA CAATGAAGGA GCAACTTCGA 
CAACATGAGA CCTCTGGGAT TGATTTGGAA GAGAAGGAAG AAATGGATAG CACTGAGGGC 
CTGAAGGGGC CAGTGAAGAA CAAGGAGAAG TCCAGAGCTG CAAAGGAGGA AAAAAAGAAG 
AAAAATCAGA ACCCTGGCCC TGGCCAGGGA TCGGAGGCTC CTGAGAAGAA GAAAGCCAAG 
ATCGACGAGC TTAAGGTGTA TCCCAAGGAG CTAGAGTCGG AGTTTGACAA CTTTGAGGAC 
TGGCTGCACA CGTTCAACCT GTTACGGGGC AAGACTGGGG ATGATGAGGA TGGCTCCACA 
GAGGAAGAAC GCATAGTAGG CCGATTCAAG GGTTCCCTCT GTGTGTACAA AGTGCCACTC 
CCGGAGGATG TGTCTCGTGA AGCTGGCTAC GATCCCACCT ACGGAATGTT CCAGGGCATC 
CCAAGCAATG ACCCCATTAA TGTGCTGGTC CGAATCTATG TGGTCCGGGC CACAGACCTG 
CACCCGGCTG ACATCAATGG CAAAGCCGAC CCCTATATCG CCATCAAGTT AGGCAAGACT 
GACATCCGAG ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGAAAGTCC 
TTTGACATTG AGGCCTCCTT CCCCATGGAG TCCATGCTGA CAGTGGCTGT GTACGACTGG 
GATCTGGTGG GCACTGATGA CCTCATCGGA GAAACCAAGA TCGACCTGGA AAACCGCTTC 
TACAGCAAGC ACCGTGCTAC CTGCGGCATC GCGCAGACCT ATTCCATACA TGGCTATAAT 
ATCTGGAGAG ACCCCATGAA GCCCAGCCAG ATCCTGACCC GCCTCTGCAA AGAGGGCAAA 
GTGGACGGCC CCCACTTTGG TCCCCATGGG AGAGTGAAGG TCGCCAACCG TGTCTTCACA 
GGGCCTTCAG AGATAGAGGA TGAGAATGGT CAGAGGAAGC CCACAGATGA GCACGTGGCA 
CTGTCTGCTC TGAGACACTG GGAGGACATC CCCCGGGTGG GCTGCCGCCT TGTACCGGAG 
CACGTGGAGA CCAGGCCGTT GCTCAACCCT GACAAGCCAG GCATTGAGCA GGGCCGCCTG 
GAGCTGTGGG TGGATATGTT CCCCATGGAT ATGCCGGCCC CTGGAACACC TCTGGATATC 
TCCCCCAGGA AACCCAAGAA GTATGAGCTG CGCGTCATCG TGTGGAACAC AGATGAGGTG 
GTCCTGGAAG ACGACGATTT CTTCACGGGA GAGAAGTCCA GTGATATTTT TGTGAGGGGG 
TGGCTGAAGG GCCAGCAGGA GGACAAACAG GACACAGACG TACATTATCA CTCCCTCACG 
GGTGAGGGCA ACTTCAACTG GCGATACCTG TTCCCCTTCG ACTACCTGGC GGCCGAAGAG 
AAGATCGTTA TGTCCAAGAA GGAGTCCATG TTCTCCTGGG ATGAGACGGA GTACAAGATA 
CCTGCGCGGC TCACCCTGCA GATCTGGGAC GCTGACCACT TCTCAGCCGA TGACTTCCTG 
GGGGCCATCG AGCTGGACCT GAACCGGTTC CCGAGGGGCG CCAAGACAGC CAAGCAGTGC 
ACCATGGAGA TGGCCACCGG GGAGGTGGAT GTGCCCCTGG TTTCCATCTT TAAACAGAAA 
CGTGTCAAAG GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ATGATGAATT TGAACTCACG 
GGCAAAGTAG AGGCAGAGTT ACACCTCCTC ACGGCAGAGG AGGCGGAGAA GAACCCGGTG 
GGCCTGGCTC GCAATGAACC TGACCCTCTA GAAAAACCCA ACCGACCCGA CACGGCGTTC 
GTCTGGTTCC TGAACCCACT CAAATCTATC AAGTACCTCA TTTGCACCCG GTACAAGTGG 
CTTATCATCA AGATTGTGCT GGCCCTGCTT GGGCTGCTCA TGCTGGCCCT CTTCCTCTAT 
AGCCTCCCAG GCTACATGGT CAAGAAGCTC CTAGGGGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Otof ( XM_008764535.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762757.1 Rattus norvegicus otoferlin (Otof), transcript variant X1, mRNA.

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CloneID	ORa43851
Clone ID Related Accession (Same CDS sequence)	XM_008764535.1
Accession Version	XM_008764535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGTCAGGCT TCTCCGGGGC TACCACATGC TGTGCAATGG GGGCCCCTCT 
TCTTGCCCCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAACCGA 
CCCGACACGG CGTTCGTCTG GTTCCTGAAC CCACTCAAAT CTATCAAGTA CCTCATTTGC 
ACCCGGTACA AGTGGCTTAT CATCAAGATT GTGCTGGCCC TGCTTGGGCT GCTCATGCTG 
GCCCTCTTCC TCTATAGCCT CCCAGGCTAC ATGGTCAAGA AGCTCCTAGG GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762757.1
CDS	173..6169
Translation	MALIVHLKTVSELRGRADRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEVLEIQIFNYSKVFSNKLIGTFRMVLQKVVEENRVEVSDTLIDDNNAIIKTSLSMEVRYQAADGTVGPWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSTRTPGEKSFRRAGRSVFSAMKLGKTRSHKEEPQRQDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTSNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISAGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLMLGLAVEILDTSNPELTSSTEVQVEQATPVSESCTGRMEEFFLFGAFLEASMIDRKNGDKPVTFEVTIGNYGNEVDGTSRPQRPRPRKEPGDEEEVDLIQNSSDDEGDEAGDLASVSSTPPMRPQITDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDVQEMIKTEKSYPERRLRGVLEELSCGCHRFLSLSDKDQGHSSRTRLDRERLKSCMRELESMGQQAKSLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDVFIWMMSNNKRIAYARVPSKDLLFSIVEEELGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKLELYLWLGLSKQRKDFLCGLPCGFEEVKAAQGLGLHSFPPISLVYTKKQTFQLRAHMYQARSLFAADSTGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPSGKADLPPINGPVDMDRGPIMPVPVGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAANWNTTVRLLRGYHMLCNGGPSSCPTGEVVVSMEPEVPVKKLETLVKLDATSDAVVKVDVAEDEKERKKKKKKGPSEEAEEEEPDESMLDWWSKYFASIDTMKEQLRQHETSGIDLEEKEEMDSTEGLKGPVKNKEKSRAAKEEKKKKNQNPGPGQGSEAPEKKKAKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDPTYGMFQGIPSNDPINVLVRIYVVRATDLHPADINGKADPYIAIKLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSIHGYNIWRDPMKPSQILTRLCKEGKVDGPHFGPHGRVKVANRVFTGPSEIEDENGQRKPTDEHVALSALRHWEDIPRVGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIVWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVMSKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLALFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_008764535.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus otoferlin (Otof), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764535.1

ORF Insert Sequence:

ATGGCCCTGA TCGTCCACCT CAAGACTGTT TCTGAGCTTC GAGGCAGAGC TGATCGGATC 
GCCAAAGTCA CTTTCCGAGG ACAGTCCTTC TATTCCCGGG TCCTGGAGAA CTGCGAGGAT 
GTGGCTGACT TCGATGAGAC ATTCCGGTGG CCAGTGGCTA GCAGCATCGA CAGGAATGAA 
GTGTTGGAGA TTCAGATTTT CAACTACAGC AAAGTCTTCA GCAACAAACT AATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTGGAG GAGAATCGGG TAGAGGTGTC TGACACGCTG 
ATCGATGACA ACAATGCTAT CATCAAGACC AGCCTAAGCA TGGAGGTCCG GTATCAAGCC 
GCAGATGGCA CAGTAGGCCC TTGGGATGAT GGAGACTTTC TGGGAGATGA ATCCCTTCAG 
GAGGAAGAGA AAGACAGCCA GGAGACAGAT GGTCTGCTAC CTGGCTCCCG ACCCAGCACC 
CGGACACCCG GCGAGAAGAG CTTTCGAAGA GCGGGAAGGA GTGTATTCTC GGCCATGAAA 
CTCGGCAAGA CTCGGTCCCA CAAAGAGGAG CCCCAAAGAC AAGATGAGCC AGCGGTGCTG 
GAGATGGAGG ACCTGGACCA CCTAGCCATC CGGCTGGGGG ATGGGCTGGA TCCTGACTCA 
GTGTCTCTAG CCTCGGTCAC TGCTCTCACC AGCAATGTCT CCAACAAACG GTCTAAGCCA 
GATATTAAAA TGGAACCGAG TGCTGGGAGA CCCATGGATT ACCAGGTCAG CATCACAGTG 
ATTGAGGCTC GGCAGCTGGT GGGTTTGAAC ATGGACCCCG TGGTGTGCGT GGAGGTGGGC 
GATGACAAAA AATACACATC CATGAAGGAG TCCACAAACT GCCCTTACTA CAACGAGTAC 
TTCGTCTTTG ACTTCCACGT CTCTCCTGAT GTCATGTTTG ACAAGATCAT CAAGATCTCG 
GTTATCCATT CTAAGAACCT GCTTCGAAGC GGCACTCTGG TGGGCTCCTT CAAAATGGAT 
GTGGGGACCG TGTACTCCCA GCCTGAACAC CAGTTCCATC ACAAATGGGC TATCCTGTCA 
GACCCCGATG ACATCTCTGC CGGGCTGAAG GGCTATGTAA AGTGTGATGT CGCCGTGGTG 
GGCAAGGGAG ACAACATCAA GACACCCCAC AAGGCCAACG AGACAGATGA GGATGACATT 
GAAGGGAACT TGCTGCTCCC CGAGGGCGTG CCCCCAGAAC GGCAGTGGGC CCGGTTCTAC 
GTGAAAATTT ACCGAGCAGA GGGACTGCCC CGGATGAACA CAAGCCTCAT GGCCAACGTA 
AAGAAGGCGT TCATCGGTGA GAACAAGGAC CTCGTCGACC CCTACGTTCA AGTCTTCTTT 
GCTGGACAGA AGGGCAAAAC ATCAGTGCAG AAGAGCAGCT ATGAGCCGCT GTGGAATGAG 
CAGGTCGTCT TTACAGATCT GTTCCCCCCA CTCTGCAAGC GCATGAAGGT GCAGATCCGA 
GACTCCGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATCGACCT GCGCAAGATT 
TCCAACGATG GAGACAAAGG CTTCCTGCCT ACGCTCGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GCAACTACAC GCTGCTGGAC GAGCACCAGG ACCTGAATGA GGGCCTGGGT 
GAGGGGGTGT CCTTCCGTGC CCGCCTCATG TTGGGACTGG CTGTGGAGAT CCTAGACACC 
TCCAACCCAG AGCTCACCAG CTCCACCGAG GTGCAGGTGG AGCAGGCCAC ACCTGTCTCA 
GAGAGCTGCA CAGGGAGAAT GGAAGAATTT TTCCTATTTG GAGCCTTCCT GGAAGCCTCA 
ATGATCGACC GGAAAAATGG GGACAAGCCA GTGACCTTTG AGGTGACCAT AGGAAACTAT 
GGCAATGAAG TCGATGGTAC ATCCCGGCCC CAGAGGCCTC GGCCCCGGAA GGAACCTGGG 
GATGAAGAAG AGGTAGACCT GATTCAGAAC TCCAGTGACG ACGAGGGTGA TGAAGCCGGG 
GACCTGGCCT CAGTGTCCTC CACCCCACCT ATGAGGCCCC AGATCACAGA CAGGAACTAT 
TTCCACCTGC CCTACCTAGA GCGCAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCTGAC 
CAGCGGCGCC GTCTCTACAA CGCAAACATC ATGGACCACA TTGCTGACAA GCTGGAAGAA 
GGTCTGAATG ATGTCCAGGA GATGATCAAA ACGGAGAAGT CCTACCCGGA GCGACGCCTG 
CGGGGTGTGC TAGAGGAACT CAGCTGTGGC TGCCACCGCT TCCTCTCCCT CTCGGACAAG 
GACCAGGGTC ACTCGTCCCG CACCAGGCTG GATCGGGAGC GCCTCAAATC CTGTATGAGG 
GAGCTGGAGA GCATGGGACA ACAAGCCAAG AGCCTGAGGG CTCAGGTGAA GCGACACACT 
GTACGGGACA AGCTGAGGTT ATGCCAGAAC TTCCTGCAGA AGCTACGCTT CCTAGCTGAT 
GAGCCCCAGC ACAGCATCCC CGATGTGTTC ATTTGGATGA TGAGTAATAA CAAACGTATC 
GCCTACGCCC GCGTGCCTTC CAAAGACCTT TTGTTCTCCA TTGTGGAGGA GGAATTGGGC 
AAGGACTGCG CCAAGGTCAA GACCCTCTTC CTGAAGCTGC CAGGAAAGAG GGGCTTCGGC 
TCGGCAGGCT GGACAGTACA GGCCAAGCTG GAACTCTACC TGTGGCTGGG CCTCAGCAAG 
CAGAGAAAGG ACTTCCTGTG TGGCCTGCCT TGTGGCTTCG AGGAGGTCAA GGCAGCCCAA 
GGCCTGGGTC TGCATTCCTT TCCGCCCATC AGCCTTGTCT ACACCAAGAA GCAAACCTTC 
CAGCTCCGAG CCCACATGTA TCAGGCCCGA AGCCTCTTCG CCGCTGACAG CACTGGGCTC 
TCTGATCCTT TTGCCCGAGT CTTCTTCATC AACCAGAGCC AGTGCACGGA GGTCCTAAAC 
GAGACACTGT GTCCTACTTG GGACCAAATG CTGGTATTTG ACAACCTGGA GCTGTACGGT 
GAAGCTCATG AGTTACGAGA TGATCCCCCC ATCATTGTCA TTGAAATCTA CGACCAGGAC 
AGCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTTGCCA AGCCCCTGGT GAAGATGGCA 
GACGAGGCAT ATTGCCCGCC TCGCTTCCCG CCCCAGCTTG AGTACTACCA GATCTACCGA 
GGCAACGCCA CGGCCGGAGA CCTACTGGCT GCCTTTGAGC TGCTGCAGAT TGGACCATCG 
GGGAAGGCTG ACCTGCCACC CATCAATGGC CCAGTGGACA TGGACAGAGG GCCCATCATG 
CCTGTGCCTG TGGGAATCCG GCCCGTGCTC AGCAAGTACA GAGTGGAGGT GCTGTTCTGG 
GGCCTGAGGG ACTTAAAGAG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCGG GAAAAGGGGT ACAATCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TGGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAATGAGCT CCTGCACCCA 
CCCTTGAACA TCCGGGTGGT GGACTGCCGG GCCTTTGGAC GATACACCCT GGTGGGCTCC 
CATGCAGTCA GCTCACTACG ACGCTTCATC TACCGACCCC CGGACCGCTC GGCCGCCAAC 
TGGAACACCA CAGTCAGGCT TCTCCGGGGC TACCACATGC TGTGCAATGG GGGCCCCTCT 
TCTTGCCCCA CAGGGGAGGT TGTAGTGAGC ATGGAACCTG AAGTGCCAGT TAAGAAGCTG 
GAGACCCTGG TGAAGCTGGA TGCGACTTCT GATGCTGTGG TCAAGGTGGA TGTGGCTGAA 
GATGAGAAGG AAAGGAAGAA GAAGAAAAAG AAAGGCCCAT CAGAGGAGGC AGAGGAGGAG 
GAACCCGATG AGAGCATGCT GGATTGGTGG TCCAAGTACT TTGCCTCCAT TGACACAATG 
AAGGAGCAAC TTCGACAACA TGAGACCTCT GGGATTGATT TGGAAGAGAA GGAAGAAATG 
GATAGCACTG AGGGCCTGAA GGGGCCAGTG AAGAACAAGG AGAAGTCCAG AGCTGCAAAG 
GAGGAAAAAA AGAAGAAAAA TCAGAACCCT GGCCCTGGCC AGGGATCGGA GGCTCCTGAG 
AAGAAGAAAG CCAAGATCGA CGAGCTTAAG GTGTATCCCA AGGAGCTAGA GTCGGAGTTT 
GACAACTTTG AGGACTGGCT GCACACGTTC AACCTGTTAC GGGGCAAGAC TGGGGATGAT 
GAGGATGGCT CCACAGAGGA AGAACGCATA GTAGGCCGAT TCAAGGGTTC CCTCTGTGTG 
TACAAAGTGC CACTCCCGGA GGATGTGTCT CGTGAAGCTG GCTACGATCC CACCTACGGA 
ATGTTCCAGG GCATCCCAAG CAATGACCCC ATTAATGTGC TGGTCCGAAT CTATGTGGTC 
CGGGCCACAG ACCTGCACCC GGCTGACATC AATGGCAAAG CCGACCCCTA TATCGCCATC 
AAGTTAGGCA AGACTGACAT CCGAGACAAG GAGAACTACA TCTCCAAGCA GCTCAACCCT 
GTCTTTGGAA AGTCCTTTGA CATTGAGGCC TCCTTCCCCA TGGAGTCCAT GCTGACAGTG 
GCTGTGTACG ACTGGGATCT GGTGGGCACT GATGACCTCA TCGGAGAAAC CAAGATCGAC 
CTGGAAAACC GCTTCTACAG CAAGCACCGT GCTACCTGCG GCATCGCGCA GACCTATTCC 
ATACATGGCT ATAATATCTG GAGAGACCCC ATGAAGCCCA GCCAGATCCT GACCCGCCTC 
TGCAAAGAGG GCAAAGTGGA CGGCCCCCAC TTTGGTCCCC ATGGGAGAGT GAAGGTCGCC 
AACCGTGTCT TCACAGGGCC TTCAGAGATA GAGGATGAGA ATGGTCAGAG GAAGCCCACA 
GATGAGCACG TGGCACTGTC TGCTCTGAGA CACTGGGAGG ACATCCCCCG GGTGGGCTGC 
CGCCTTGTAC CGGAGCACGT GGAGACCAGG CCGTTGCTCA ACCCTGACAA GCCAGGCATT 
GAGCAGGGCC GCCTGGAGCT GTGGGTGGAT ATGTTCCCCA TGGATATGCC GGCCCCTGGA 
ACACCTCTGG ATATCTCCCC CAGGAAACCC AAGAAGTATG AGCTGCGCGT CATCGTGTGG 
AACACAGATG AGGTGGTCCT GGAAGACGAC GATTTCTTCA CGGGAGAGAA GTCCAGTGAT 
ATTTTTGTGA GGGGGTGGCT GAAGGGCCAG CAGGAGGACA AACAGGACAC AGACGTACAT 
TATCACTCCC TCACGGGTGA GGGCAACTTC AACTGGCGAT ACCTGTTCCC CTTCGACTAC 
CTGGCGGCCG AAGAGAAGAT CGTTATGTCC AAGAAGGAGT CCATGTTCTC CTGGGATGAG 
ACGGAGTACA AGATACCTGC GCGGCTCACC CTGCAGATCT GGGACGCTGA CCACTTCTCA 
GCCGATGACT TCCTGGGGGC CATCGAGCTG GACCTGAACC GGTTCCCGAG GGGCGCCAAG 
ACAGCCAAGC AGTGCACCAT GGAGATGGCC ACCGGGGAGG TGGATGTGCC CCTGGTTTCC 
ATCTTTAAAC AGAAACGTGT CAAAGGCTGG TGGCCCCTCC TGGCCCGCAA TGAGAATGAT 
GAATTTGAAC TCACGGGCAA AGTAGAGGCA GAGTTACACC TCCTCACGGC AGAGGAGGCG 
GAGAAGAACC CGGTGGGCCT GGCTCGCAAT GAACCTGACC CTCTAGAAAA ACCCAACCGA 
CCCGACACGG CGTTCGTCTG GTTCCTGAAC CCACTCAAAT CTATCAAGTA CCTCATTTGC 
ACCCGGTACA AGTGGCTTAT CATCAAGATT GTGCTGGCCC TGCTTGGGCT GCTCATGCTG 
GCCCTCTTCC TCTATAGCCT CCCAGGCTAC ATGGTCAAGA AGCTCCTAGG GGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Function coupling of otoferlin with GAD65 acts to modulate GABAergic activity.
Journal of molecular cell biology7(2)168-79(2015 Apr)
Wu W,Rahman MN,Guo J,Roy N,Xue L,Cahill CM,Zhang S,Jia Z

Unconventional molecular regulation of synaptic vesicle replenishment in cochlear inner hair cells.
Journal of cell science128(4)638-44(2015 Feb)
Vogl C,Cooper BH,Neef J,Wojcik SM,Reim K,Reisinger E,Brose N,Rhee JS,Moser T,Wichmann C

Calcium regulates molecular interactions of otoferlin with soluble NSF attachment protein receptor (SNARE) proteins required for hair cell exocytosis.
The Journal of biological chemistry289(13)8750-66(2014 Mar)
Ramakrishnan NA,Drescher MJ,Morley BJ,Kelley PM,Drescher DG

Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(22)9508-19(2013 May)
Duncker SV,Franz C,Kuhn S,Schulte U,Campanelli D,Brandt N,Hirt B,Fakler B,Blin N,Ruth P,Engel J,Marcotti W,Zimmermann U,Knipper M

Screening of OTOF mutations in Iran: a novel mutation and review.
International journal of pediatric otorhinolaryngology76(11)1610-5(2012 Nov)
Mahdieh N,Shirkavand A,Rabbani B,Tekin M,Akbari B,Akbari MT,Zeinali S

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Otof cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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