OTOF gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015285201.1	XP_015140687.1	otoferlin isoform X4
XM_015285202.1	XP_015140688.1	otoferlin isoform X5
XM_015285205.1	XP_015140691.1	otoferlin isoform X11
XM_015285198.2	XP_015140684.1	otoferlin isoform X1
XM_420015.6	XP_420015.2	otoferlin isoform X8
XM_015285206.2	XP_015140692.1	otoferlin isoform X11
XM_004935857.2	XP_004935914.1	otoferlin isoform X9
XM_015285207.1	XP_015140693.1	otoferlin isoform X12
XM_015285200.2	XP_015140686.1	otoferlin isoform X3
XM_015285202.2	XP_015140688.1	otoferlin isoform X5
XM_015285204.2	XP_015140690.1	otoferlin isoform X7
XM_015285205.2	XP_015140691.1	otoferlin isoform X11
XM_015285198.1	XP_015140684.1	otoferlin isoform X1
XM_015285204.1	XP_015140690.1	otoferlin isoform X7
XM_015285199.2	XP_015140685.1	otoferlin isoform X2
XM_004935857.3	XP_004935914.1	otoferlin isoform X9
XM_420015.5	XP_420015.2	otoferlin isoform X8
XM_004935859.2	XP_004935916.1	otoferlin isoform X10
XM_015285201.2	XP_015140687.1	otoferlin isoform X4
XM_015285207.2	XP_015140693.1	otoferlin isoform X12
XM_015285199.1	XP_015140685.1	otoferlin isoform X2
XM_015285200.1	XP_015140686.1	otoferlin isoform X3
XM_015285203.1	XP_015140689.1	otoferlin isoform X6
XM_015285206.1	XP_015140692.1	otoferlin isoform X11
XM_015285203.2	XP_015140689.1	otoferlin isoform X6
XM_004935859.3	XP_004935916.1	otoferlin isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa34479	XM_015285206.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa34479	XM_015285205.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa34480	XM_015285207.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa07078	XM_004935859.3 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa34472	XM_015285198.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34473	XM_015285199.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34474	XM_015285200.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34475	XM_015285201.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34476	XM_015285202.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34477	XM_015285203.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34478	XM_015285204.2 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa07075	XM_420015.6 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa07076	XM_004935857.3 Latest version!	Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OGa34476	XM_015285202.1	Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34479	XM_015285205.1	Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa34472	XM_015285198.1	Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34478	XM_015285204.1	Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa07076	XM_004935857.2	Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa07075	XM_420015.5	Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OGa34475	XM_015285201.1	Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34480	XM_015285207.1	Gallus gallus otoferlin (OTOF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa34473	XM_015285199.1	Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34474	XM_015285200.1	Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa34479	XM_015285206.1	Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OGa34477	XM_015285203.1	Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OGa07078	XM_004935859.2	Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

OTOF ( XM_015285205.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140691.1 Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa34479
Clone ID Related Accession (Same CDS sequence)	XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
Accession Version	XM_015285205.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140691.1
CDS	553..6051
Translation	MKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285205.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285205.1

ORF Insert Sequence:

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285206.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140692.1 Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.

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CloneID	OGa34479
Clone ID Related Accession (Same CDS sequence)	XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
Accession Version	XM_015285206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140692.1
CDS	155..5653
Translation	MKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285206.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285206.2

ORF Insert Sequence:

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285205.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140691.1 Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.

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CloneID	OGa34479
Clone ID Related Accession (Same CDS sequence)	XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
Accession Version	XM_015285205.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140691.1
CDS	386..5884
Translation	MKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285205.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285205.2

ORF Insert Sequence:

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285206.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140692.1 Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.

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CloneID	OGa34479
Clone ID Related Accession (Same CDS sequence)	XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
Accession Version	XM_015285206.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140692.1
CDS	162..5660
Translation	MKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285206.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285206.1

ORF Insert Sequence:

ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG 
GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC 
AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC 
ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG 
GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT 
GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG 
CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG 
ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC 
CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT 
GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT 
GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT 
TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC 
AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG 
GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG 
AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG 
ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG 
AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC 
ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG 
CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG 
GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA 
GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA 
GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC 
AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA 
GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT 
GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC 
AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG 
TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG 
CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG 
CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG 
GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC 
TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG 
AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC 
TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC 
AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG 
GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG 
GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC 
CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG 
ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA 
CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT 
AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG 
GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG 
CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT 
GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG 
AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG 
ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT 
GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC 
CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC 
TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT 
GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA 
AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG 
CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG 
GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA 
GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG 
GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC 
AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT 
GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA 
GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG 
TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA 
GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC 
ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC 
AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT 
GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG 
CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA 
GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT 
GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC 
AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC 
CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC 
ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT 
GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC 
TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC 
AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC 
TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG 
GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT 
CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG 
ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC 
GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG 
ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC 
CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC 
CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG 
CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC 
GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG 
ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA 
GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG 
GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT 
CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG 
GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG 
AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG 
CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC 
TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC 
ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC 
ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285207.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140693.1 Gallus gallus otoferlin (OTOF), transcript variant X13, mRNA.

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CloneID	OGa34480
Clone ID Related Accession (Same CDS sequence)	XM_015285207.1 , XM_015285207.2
Accession Version	XM_015285207.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTA CCTGCCTCAC GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140693.1
CDS	155..5461
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGTCLTG

Target ORF information:

RefSeq Version	XM_015285207.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285207.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTA CCTGCCTCAC GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285207.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140693.1 Gallus gallus otoferlin (OTOF), transcript variant X14, mRNA.

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CloneID	OGa34480
Clone ID Related Accession (Same CDS sequence)	XM_015285207.1 , XM_015285207.2
Accession Version	XM_015285207.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTA CCTGCCTCAC GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140693.1
CDS	152..5458
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGTCLTG

Target ORF information:

RefSeq Version	XM_015285207.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285207.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTA CCTGCCTCAC GGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_004935859.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935916.1 Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.

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CloneID	OGa07078
Clone ID Related Accession (Same CDS sequence)	XM_004935859.2 , XM_004935859.3
Accession Version	XM_004935859.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935916.1
CDS	152..6124
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_004935859.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935859.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_004935859.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935916.1 Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.

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CloneID	OGa07078
Clone ID Related Accession (Same CDS sequence)	XM_004935859.2 , XM_004935859.3
Accession Version	XM_004935859.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935916.1
CDS	1..5973
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_004935859.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935859.3

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285198.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140684.1 Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OGa34472
Clone ID Related Accession (Same CDS sequence)	XM_015285198.2 , XM_015285198.1
Accession Version	XM_015285198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140684.1
CDS	149..6469
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285198.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285198.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285198.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140684.1 Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OGa34472
Clone ID Related Accession (Same CDS sequence)	XM_015285198.2 , XM_015285198.1
Accession Version	XM_015285198.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140684.1
CDS	153..6473
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285198.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285198.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285199.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140685.1 Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.

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CloneID	OGa34473
Clone ID Related Accession (Same CDS sequence)	XM_015285199.2 , XM_015285199.1
Accession Version	XM_015285199.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140685.1
CDS	150..6440
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285199.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285199.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285199.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140685.1 Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.

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CloneID	OGa34473
Clone ID Related Accession (Same CDS sequence)	XM_015285199.2 , XM_015285199.1
Accession Version	XM_015285199.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140685.1
CDS	153..6443
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285199.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285199.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285200.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140686.1 Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.

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CloneID	OGa34474
Clone ID Related Accession (Same CDS sequence)	XM_015285200.2 , XM_015285200.1
Accession Version	XM_015285200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC 
AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG 
CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG 
GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC 
ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT 
GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC 
ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG 
GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC 
TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT 
GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC 
GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT 
GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG 
GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT 
GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC 
ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG 
AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT 
GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG 
CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA 
GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC 
TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT 
GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG 
GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC 
ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT 
GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC 
ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT 
GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT 
GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT 
GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC 
TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG 
GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG 
GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG 
CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC 
AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG 
CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT 
ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA 
GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT 
ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG 
GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC 
GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC 
AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC 
AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT 
TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC 
CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC 
AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC 
GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA 
GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG 
TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC 
CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT 
GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC 
CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT 
TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC 
ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA 
AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC 
CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC 
TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG 
CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT 
CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG 
ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT 
GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA 
GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT 
GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA 
GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140686.1
CDS	149..6421
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285200.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285200.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC 
AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG 
CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG 
GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC 
ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT 
GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC 
ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG 
GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC 
TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT 
GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC 
GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT 
GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG 
GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT 
GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC 
ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG 
AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT 
GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG 
CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA 
GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC 
TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT 
GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG 
GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC 
ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT 
GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC 
ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT 
GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT 
GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT 
GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC 
TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG 
GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG 
GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG 
CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC 
AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG 
CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT 
ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA 
GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT 
ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG 
GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC 
GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC 
AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC 
AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT 
TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC 
CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC 
AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC 
GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA 
GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG 
TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC 
CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT 
GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC 
CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT 
TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC 
ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA 
AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC 
CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC 
TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG 
CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT 
CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG 
ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT 
GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA 
GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT 
GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA 
GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285200.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140686.1 Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.

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CloneID	OGa34474
Clone ID Related Accession (Same CDS sequence)	XM_015285200.2 , XM_015285200.1
Accession Version	XM_015285200.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC 
AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG 
CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG 
GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC 
ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT 
GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC 
ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG 
GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC 
TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT 
GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC 
GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT 
GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG 
GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT 
GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC 
ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG 
AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT 
GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG 
CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA 
GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC 
TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT 
GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG 
GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC 
ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT 
GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC 
ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT 
GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT 
GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT 
GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC 
TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG 
GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG 
GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG 
CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC 
AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG 
CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT 
ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA 
GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT 
ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG 
GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC 
GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC 
AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC 
AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT 
TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC 
CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC 
AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC 
GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA 
GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG 
TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC 
CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT 
GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC 
CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT 
TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC 
ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA 
AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC 
CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC 
TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG 
CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT 
CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG 
ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT 
GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA 
GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT 
GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA 
GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140686.1
CDS	153..6425
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285200.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285200.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC 
AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG 
CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG 
GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC 
ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT 
GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC 
ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG 
GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC 
TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT 
GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC 
GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT 
GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG 
GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT 
GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC 
ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG 
AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT 
GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG 
CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA 
GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC 
TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT 
GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG 
GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC 
ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT 
GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC 
ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT 
GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT 
GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT 
GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC 
TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG 
GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG 
GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG 
CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC 
AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG 
CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT 
ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA 
GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT 
ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG 
GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC 
GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC 
AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC 
AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT 
TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC 
CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC 
AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC 
GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA 
GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG 
TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC 
CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT 
GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC 
CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT 
TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC 
ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA 
AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC 
CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC 
TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG 
CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT 
CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG 
ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT 
GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA 
GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT 
GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA 
GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285201.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140687.1 Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.

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CloneID	OGa34475
Clone ID Related Accession (Same CDS sequence)	XM_015285201.1 , XM_015285201.2
Accession Version	XM_015285201.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140687.1
CDS	153..6413
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285201.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285201.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285201.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140687.1 Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.

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CloneID	OGa34475
Clone ID Related Accession (Same CDS sequence)	XM_015285201.1 , XM_015285201.2
Accession Version	XM_015285201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140687.1
CDS	149..6409
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285201.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285201.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC 
CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG 
TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC 
AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG 
CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA 
AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG 
GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT 
GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG 
AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC 
TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC 
CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC 
CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG 
TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG 
CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG 
CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT 
GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG 
GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG 
CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC 
CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA 
TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC 
TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG 
GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG 
CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA 
GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG 
GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG 
AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC 
GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA 
TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA 
CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC 
GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC 
AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG 
GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC 
AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285202.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140688.1 Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.

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CloneID	OGa34476
Clone ID Related Accession (Same CDS sequence)	XM_015285202.1 , XM_015285202.2
Accession Version	XM_015285202.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC 
CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG 
TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA 
CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA 
GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG 
CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA 
GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC 
TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT 
TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC 
GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC 
TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT 
GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG 
GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC 
CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG 
AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT 
GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG 
AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC 
ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC 
CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC 
TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG 
CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT 
TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC 
CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA 
GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT 
GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG 
GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT 
GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT 
GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG 
AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT 
GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG 
CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC 
TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC 
AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG 
AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG 
CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA 
GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG 
AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG 
CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG 
ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT 
TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG 
CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT 
TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT 
TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT 
CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA 
TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG 
AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT 
TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT 
GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT 
GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG 
CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC 
GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA 
GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA 
TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG 
GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC 
CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC 
CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT 
GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC 
CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG 
GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA 
CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA 
GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA 
GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG 
GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG 
GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA 
CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG 
GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG 
AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT 
GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC 
AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG 
TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG 
GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG 
CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC 
TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA 
GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG 
GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA 
CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC 
AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC 
AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT 
GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG 
CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG 
CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT 
GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC 
ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT 
TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC 
CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG 
ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT 
GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC 
GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA 
TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC 
TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG 
CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG 
AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA 
GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC 
CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC 
CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140688.1
CDS	153..6386
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285202.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285202.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC 
CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG 
TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA 
CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA 
GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG 
CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA 
GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC 
TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT 
TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC 
GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC 
TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT 
GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG 
GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC 
CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG 
AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT 
GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG 
AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC 
ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC 
CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC 
TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG 
CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT 
TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC 
CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA 
GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT 
GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG 
GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT 
GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT 
GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG 
AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT 
GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG 
CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC 
TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC 
AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG 
AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG 
CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA 
GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG 
AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG 
CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG 
ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT 
TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG 
CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT 
TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT 
TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT 
CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA 
TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG 
AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT 
TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT 
GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT 
GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG 
CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC 
GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA 
GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA 
TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG 
GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC 
CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC 
CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT 
GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC 
CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG 
GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA 
CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA 
GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA 
GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG 
GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG 
GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA 
CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG 
GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG 
AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT 
GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC 
AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG 
TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG 
GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG 
CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC 
TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA 
GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG 
GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA 
CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC 
AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC 
AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT 
GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG 
CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG 
CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT 
GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC 
ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT 
TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC 
CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG 
ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT 
GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC 
GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA 
TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC 
TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG 
CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG 
AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA 
GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC 
CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC 
CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285202.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140688.1 Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.

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CloneID	OGa34476
Clone ID Related Accession (Same CDS sequence)	XM_015285202.1 , XM_015285202.2
Accession Version	XM_015285202.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC 
CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG 
TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA 
CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA 
GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG 
CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA 
GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC 
TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT 
TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC 
GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC 
TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT 
GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG 
GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC 
CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG 
AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT 
GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG 
AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC 
ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC 
CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC 
TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG 
CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT 
TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC 
CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA 
GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT 
GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG 
GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT 
GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT 
GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG 
AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT 
GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG 
CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC 
TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC 
AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG 
AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG 
CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA 
GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG 
AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG 
CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG 
ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT 
TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG 
CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT 
TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT 
TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT 
CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA 
TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG 
AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT 
TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT 
GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT 
GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG 
CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC 
GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA 
GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA 
TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG 
GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC 
CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC 
CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT 
GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC 
CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG 
GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA 
CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA 
GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA 
GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG 
GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG 
GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA 
CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG 
GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG 
AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT 
GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC 
AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG 
TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG 
GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG 
CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC 
TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA 
GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG 
GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA 
CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC 
AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC 
AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT 
GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG 
CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG 
CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT 
GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC 
ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT 
TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC 
CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG 
ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT 
GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC 
GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA 
TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC 
TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG 
CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG 
AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA 
GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC 
CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC 
CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140688.1
CDS	149..6382
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285202.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285202.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC 
CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG 
TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA 
CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA 
GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG 
CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA 
GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC 
TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT 
TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC 
GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC 
TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT 
GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG 
GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC 
CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG 
AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT 
GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG 
AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC 
ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC 
CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC 
TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG 
CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT 
TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC 
CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA 
GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT 
GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG 
GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT 
GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT 
GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG 
AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT 
GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG 
CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC 
TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC 
AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG 
AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG 
CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA 
GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG 
AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG 
CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG 
ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT 
TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG 
CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT 
TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT 
TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT 
CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA 
TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG 
AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT 
TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT 
GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT 
GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG 
CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC 
GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA 
GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA 
TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG 
GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC 
CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC 
CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT 
GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC 
CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG 
GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA 
CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA 
GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA 
GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG 
GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG 
GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA 
CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG 
GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG 
AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT 
GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC 
AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG 
TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG 
GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG 
CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC 
TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA 
GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG 
GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA 
CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC 
AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC 
AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT 
GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG 
CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG 
CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT 
GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC 
ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT 
TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC 
CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG 
ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT 
GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC 
GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA 
TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC 
TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG 
CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG 
AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA 
GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC 
CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC 
CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285203.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140689.1 Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.

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CloneID	OGa34477
Clone ID Related Accession (Same CDS sequence)	XM_015285203.1 , XM_015285203.2
Accession Version	XM_015285203.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140689.1
CDS	153..6383
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285203.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285203.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285203.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140689.1 Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.

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CloneID	OGa34477
Clone ID Related Accession (Same CDS sequence)	XM_015285203.1 , XM_015285203.2
Accession Version	XM_015285203.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140689.1
CDS	149..6379
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRMEEDEMYYHSEDELLGEGDTLSQGSLNTSLGDDAEEEQHTRIKFNDGYKSVASEDPEDTRAKAKGKEKRHLMLPFSKGKEKTKEHKAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285203.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285203.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG 
ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC 
CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT 
AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA 
GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG 
AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA 
CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC 
CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC 
TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG 
GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG 
CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA 
TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT 
TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA 
AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT 
TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC 
CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC 
AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC 
CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT 
CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC 
ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG 
GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC 
ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA 
GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA 
GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG 
AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC 
TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG 
ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT 
GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG 
AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT 
GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG 
GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC 
TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG 
CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC 
AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT 
GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC 
CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC 
CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG 
AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC 
AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG 
CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC 
CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC 
GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC 
TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA 
GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC 
CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA 
GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC 
TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG 
TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT 
GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC 
AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG 
TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT 
ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA 
GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA 
CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG 
GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT 
GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT 
TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC 
ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC 
AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG 
ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG 
GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG 
GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA 
GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG 
ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA 
GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA 
CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC 
AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC 
TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC 
AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA 
GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC 
CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC 
ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG 
GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC 
GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG 
TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG 
AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT 
GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA 
GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG 
GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA 
CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG 
GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG 
GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC 
ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA 
GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT 
GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC 
TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG 
GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG 
TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT 
GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC 
AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC 
AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG 
GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA 
TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC 
ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC 
TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC 
TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285204.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140690.1 Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.

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CloneID	OGa34478
Clone ID Related Accession (Same CDS sequence)	XM_015285204.2 , XM_015285204.1
Accession Version	XM_015285204.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140690.1
CDS	149..6211
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285204.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285204.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_015285204.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015140690.1 Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.

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CloneID	OGa34478
Clone ID Related Accession (Same CDS sequence)	XM_015285204.2 , XM_015285204.1
Accession Version	XM_015285204.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015140690.1
CDS	153..6215
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_015285204.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015285204.1

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_420015.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420015.2 Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.

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CloneID	OGa07075
Clone ID Related Accession (Same CDS sequence)	XM_420015.6 , XM_420015.5
Accession Version	XM_420015.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420015.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420015.2
CDS	1..6033
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_420015.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420015.6

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_420015.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_420015.2 Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.

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CloneID	OGa07075
Clone ID Related Accession (Same CDS sequence)	XM_420015.6 , XM_420015.5
Accession Version	XM_420015.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420015.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_420015.2
CDS	153..6185
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTAKQLKGYLAMANGAPRSRPTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_420015.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_420015.5

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA 
GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG 
AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA 
CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG 
CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT 
ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT 
GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA 
GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT 
GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG 
GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG 
CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT 
GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG 
CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG 
GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG 
TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG 
ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG 
GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG 
AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA 
GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG 
GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC 
AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC 
ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC 
CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC 
GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG 
ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG 
GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC 
GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT 
CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC 
AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA 
GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_004935857.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935914.1 Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.

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CloneID	OGa07076
Clone ID Related Accession (Same CDS sequence)	XM_004935857.2 , XM_004935857.3
Accession Version	XM_004935857.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935914.1
CDS	152..6154
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_004935857.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935857.2

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_004935857.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004935914.1 Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.

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CloneID	OGa07076
Clone ID Related Accession (Same CDS sequence)	XM_004935857.2 , XM_004935857.3
Accession Version	XM_004935857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	otoferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004935914.1
CDS	1..6003
Translation	MALQLQLRTVSGLRGRADRIAKAAFRGLSFYTRVMENCEDEARFDETFRWPVASNIDGNEILEIQVFNYSKVFTNRLIGTFRMVLQKVVEEGHVEVTDTLIDDNNSAIQTSISIEIRYQALDGTVGTWNDKEFLETPSVHSEGDGRYPLETDSLLSGHRQSTDGSVGKSNQQSTERSFRRAGKGVFSAMKLGKARPTKDDHRKQDEPAVLEAEDLDRKVMRLGGGLDPDTISLASVTAVTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGEEKKYTSMKESTNCPYYNEYFVFDFHVPPDVMFDKIIKLSVIHSKNLLRSGTLVGSFKMDVGTVYTQPEHQFYHKWAILSDPEDLTAGLKGYLKCDIAVVGKGDNIKTPHKANETEEDDIEGNLLLPDGVPPERQWARFYIKIYRAEGLPRMNTSIMANVKKALIGENKDLVDPYVQVAFAGQKGKTSVQKSSYEPLWNEQIIFTEMFPPLCKRIKIQIRDSDKVNDVAIGTHFIDLRKISNEGDKGYLPTFGPAWVNMYGSTRNYTLMDEHQELNEGLGEGVSFRARLLMSLAVEILDTTNPEINSSTEVQVEQATPVADNCTGKMEEFFLFGAFLEATMIDRKIGDKPINFEVTIGNYGNQIDGMNKPVLRRKKEGGDGDEEESELLHNSSEDEADEDGEMVSVSSSQPMKPLVTDRNYFHLPYFEKKPCIYIKSWWQDQRRRLYNANIMDKIADKLEEGLNDVQEMIKTEKPHPERRLRGVLEELSSGCLRFVTLADKDQHHSSRTRLDRERLKSCMRELENMGQQATTLRSQVKKNTMKDKLKQVQNFLQKLRFLADEPQHTIPDIFIWMMSNNKRIAYARVPSKDILYSIVDEEMGKDCAKVKTVFLKLPGKRGFGPAGWTVQAKMEIYLWLGLNKQRKDFLSGLPCGFEEKKTTRGQNLPSFPPISLLYTKKQVFQLRAHMYQARSLFAADSSGLSDPFARVFFTSQSQCTEVLNETLCPTWDQLLVFDNVELYGEAHEMRDDPPIIVIEIYDQDTVGKADFMGRTFAKPVVKMSDEQYCPPRFPPQLEYYQIYRGNSTAGDLLAAFELLQIGSGGKSDLPPIDGPTDIDRGPILPVPLGIRPVLSKYRVEILFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSALIQNYKKNPNFSTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHTVSSLRKFIYRPPDKKAQHWNMTGEIIVNMEPEVPIKKMETMVKLEANSDAVVKVDVSEEEKEKKKKKKKGGGGGGGGEEIEEEEPDESMLDWWSKYFASIETMKEQLRQQEAAAAEAEEKEEMEIAEEIKLDDSPMKGFKGQAKNKEKSKAPKDDKKKKQQSAPELPEKKNKQKIDELKVFNKELEAEFDNFEDWLHTFNLLRGKIGDNDDNATEEERIVGRFKGSMCVYKVPLPDDITKEAGYDPTFGMFQGIPSNDPINVLVRVYIVRATDLHPADINGKADPYIAIKLGKTDIKDKENYISKQLNPVFGKSFDIEATFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRYYSKHRATCGVSQTYSIHGYNIWRDPMKPSQILSKLCKEGKVDGPHFGPGGRVKVANRVYTGPTEIEDENGQKKQTDEHLALTVLRHWEDVPRAGCKLVPEHVETRPLLNPDKPGIEQGRLEMWVDMFPMDMPAPGPAIDISPRKPKKYELRVIVWNTDEVILEDDDYFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYIFPFDYLMAEEKIVISKKESMFSWDETEYKIPARLTLQVWDADHFSADDFLGAIELDLNRFPRGAKTSKQCSLEMVTNEAELPMVSIFKQKRVKGWWPFVARDENDELEITGKVEAELHLLTAEEAEKSPAGLARNEPDPLEKPNRPDTSFIWFLNPLKSIKYLICTRYKWLIIKIVLALLLLIMVALFLYSMPGYMVKKLLGA

Target ORF information:

RefSeq Version	XM_004935857.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004935857.3

ORF Insert Sequence:

ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC 
GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG 
ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT 
TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC 
ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT 
CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC 
TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG 
AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA 
GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC 
CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG 
AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC 
ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG 
CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC 
ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA 
TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT 
GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT 
GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC 
CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA 
GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC 
AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT 
CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC 
CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC 
TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA 
AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA 
TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT 
GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC 
ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT 
GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG 
ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG 
GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC 
TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC 
ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT 
GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC 
AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG 
CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG 
CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC 
ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC 
AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC 
GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG 
TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC 
ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG 
AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC 
TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC 
ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC 
TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG 
ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG 
CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC 
ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC 
AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC 
ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG 
CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT 
CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC 
CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC 
CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG 
GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT 
GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG 
CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC 
CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA 
GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA 
GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC 
CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC 
ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC 
AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC 
TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG 
AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG 
AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC 
CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG 
GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG 
AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT 
GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA 
GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG 
GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG 
TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC 
TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT 
ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT 
GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG 
AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG 
ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG 
ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT 
TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC 
AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA 
GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA 
CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA 
GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG 
GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA 
CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA 
GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC 
AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC 
GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT 
GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG 
GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC 
TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA 
GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG 
GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG 
AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG 
GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC 
AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC 
ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC 
ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	OTOF
Entrez Gene ID	422007
Full Name	otoferlin
General protein information	Preferred Names otoferlin Names otoferlin
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	OTOF	NP_919224.1
Canis lupus familiaris (dog)	OTOF	XP_005630285.1
Bos taurus (cattle)	OTOF	NP_001137579.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000389	XP_310715.5
Xenopus tropicalis (tropical clawed frog)	otof	XP_004918245.1
Mus musculus (house mouse)	Otof	NP_114081.2
Gallus gallus (chicken)	OTOF	XP_420015.2
Danio rerio (zebrafish)	otof	NP_001025283.1
Pan troglodytes (chimpanzee)	OTOF	XP_003949828.1
	OTOF	XP_002799220.1
Rattus norvegicus (Norway rat)	Otof	NP_001263649.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

OTOF cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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