OTOF cDNA ORF clone, Gallus gallus(chicken)

The following OTOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa34479 XM_015285206.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa34479 XM_015285205.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa34480 XM_015285207.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa07078 XM_004935859.3
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa34472 XM_015285198.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34473 XM_015285199.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34474 XM_015285200.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34475 XM_015285201.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34476 XM_015285202.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34477 XM_015285203.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34478 XM_015285204.2
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa07075 XM_420015.6
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OGa07076 XM_004935857.3
Latest version!
Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OGa34476 XM_015285202.1 Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34479 XM_015285205.1 Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa34472 XM_015285198.1 Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34478 XM_015285204.1 Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa07076 XM_004935857.2 Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OGa07075 XM_420015.5 Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OGa34475 XM_015285201.1 Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34480 XM_015285207.1 Gallus gallus otoferlin (OTOF), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa34473 XM_015285199.1 Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34474 XM_015285200.1 Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa34479 XM_015285206.1 Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OGa34477 XM_015285203.1 Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OGa07078 XM_004935859.2 Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa34479
    Clone ID Related Accession (Same CDS sequence) XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
    Accession Version XM_015285205.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140691.1
    CDS553..6051
    Translation

    Target ORF information:

    RefSeq Version XM_015285205.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34479
    Clone ID Related Accession (Same CDS sequence) XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
    Accession Version XM_015285206.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140692.1
    CDS155..5653
    Translation

    Target ORF information:

    RefSeq Version XM_015285206.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34479
    Clone ID Related Accession (Same CDS sequence) XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
    Accession Version XM_015285205.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285205.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140691.1
    CDS386..5884
    Translation

    Target ORF information:

    RefSeq Version XM_015285205.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285205.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34479
    Clone ID Related Accession (Same CDS sequence) XM_015285205.1 , XM_015285206.2 , XM_015285205.2 , XM_015285206.1
    Accession Version XM_015285206.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140692.1
    CDS162..5660
    Translation

    Target ORF information:

    RefSeq Version XM_015285206.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGCTTG GCAAGGCACG TCCCACAAAA GATGACCATA GGAAGCAAGA TGAACCTGCT 
    GTTCTGGAGG CAGAAGACCT GGACCGAAAA GTCATGAGAC TGGGAGGGGG GCTGGACCCG
    GACACCATCT CCTTGGCCTC TGTCACAGCT GTCACCACCA ATGTCTCAAA CAAGAGGTCC
    AAACCTGACA TCAAAATGGA GCCAAGTGCT GGGAGGCCGA TGGATTACCA GGTCAGCATC
    ACTGTCATCG AGGCCCGGCA GCTGGTTGGG CTCAACATGG ACCCCGTGGT CTGCGTGGAG
    GTTGGGGAGG AGAAGAAATA CACATCCATG AAGGAATCGA CCAACTGCCC TTACTACAAT
    GAGTACTTTG TCTTCGATTT CCACGTGCCC CCGGATGTCA TGTTTGATAA GATCATCAAG
    CTGTCTGTGA TCCACTCAAA AAACCTCTTG CGCAGTGGGA CTTTGGTGGG CTCCTTCAAG
    ATGGACGTTG GGACAGTTTA CACCCAGCCT GAGCACCAGT TCTACCACAA ATGGGCCATC
    CTTTCTGACC CCGAGGACCT CACTGCTGGG CTGAAAGGTT ACCTGAAGTG TGACATCGCT
    GTTGTGGGGA AAGGGGACAA CATCAAGACA CCCCACAAAG CCAATGAGAC AGAGGAGGAT
    GATATTGAAG GGAACCTCCT CCTACCCGAT GGGGTTCCTC CAGAGAGGCA GTGGGCTCGT
    TTCTACATCA AGATCTATCG AGCAGAGGGC CTGCCCCGTA TGAACACCAG CATCATGGCC
    AATGTGAAGA AGGCTCTCAT TGGAGAGAAT AAGGACTTGG TAGACCCCTA CGTGCAAGTG
    GCATTTGCAG GGCAGAAGGG GAAGACATCA GTGCAAAAAA GCAGCTATGA GCCTCTCTGG
    AATGAGCAAA TCATCTTCAC AGAAATGTTC CCTCCATTGT GCAAGCGGAT AAAGATCCAG
    ATCCGAGACT CGGACAAAGT CAATGATGTG GCCATTGGTA CCCATTTCAT TGATTTGAGG
    AAGATCTCTA ATGAAGGAGA CAAAGGCTAC CTGCCCACCT TTGGCCCTGC ATGGGTGAAC
    ATGTATGGCT CCACTCGGAA CTACACCCTG ATGGATGAAC ACCAAGAGCT GAATGAGGGG
    CTGGGGGAAG GCGTCTCCTT CCGGGCCAGG CTGCTGATGA GCCTTGCGGT TGAGATCTTG
    GATACCACCA ACCCAGAGAT CAACAGCTCC ACTGAGGTGC AAGTGGAGCA GGCCACACCA
    GTTGCTGATA ACTGCACTGG AAAGATGGAG GAGTTCTTTC TTTTTGGAGC CTTCCTGGAA
    GCTACCATGA TCGACAGGAA GATCGGTGAC AAACCCATCA ACTTTGAGGT TACAATAGGC
    AACTATGGGA ACCAGATTGA TGGCATGAAC AAACCTGTCC TGCGGAGGAA GAAGGAGGGA
    GGGGATGGGG ACGAGGAGGA GTCCGAGCTG CTACACAACT CAAGTGAGGA TGAAGCTGAT
    GAGGATGGAG AGATGGTCTC TGTCTCCTCG TCTCAGCCCA TGAAGCCCCT GGTTACAGAC
    AGGAACTACT TCCACCTGCC GTACTTTGAG AAGAAGCCCT GCATCTACAT CAAGAGCTGG
    TGGCAGGATC AGCGCCGCAG GCTGTATAAC GCCAACATCA TGGACAAGAT TGCAGACAAG
    CTGGAGGAAG GGCTCAATGA TGTGCAGGAA ATGATCAAGA CAGAGAAACC ACATCCAGAG
    CGCCGGCTCC GAGGGGTCCT GGAGGAGCTG AGCAGCGGGT GCTTGCGCTT TGTGACGCTG
    GCTGACAAGG ACCAGCACCA CTCTTCCCGC ACGAGGTTGG ACCGAGAGAG GCTCAAGTCC
    TGCATGCGGG AGCTGGAGAA CATGGGCCAG CAAGCCACGA CTCTGAGGTC GCAGGTGAAG
    AAGAATACCA TGAAAGACAA GCTGAAGCAG GTGCAGAACT TCCTGCAGAA ACTCCGTTTC
    TTGGCAGATG AGCCCCAGCA CACCATCCCT GACATCTTCA TCTGGATGAT GAGCAATAAC
    AAGCGTATTG CCTATGCCCG TGTCCCTTCC AAGGACATCC TCTATTCCAT TGTGGATGAG
    GAGATGGGCA AGGACTGCGC CAAAGTGAAG ACTGTCTTTC TCAAGCTGCC TGGGAAGAGG
    GGTTTCGGAC CTGCCGGCTG GACAGTTCAG GCCAAGATGG AGATTTACTT GTGGCTGGGC
    CTCAACAAGC AGCGCAAAGA CTTCCTGAGT GGCTTGCCCT GTGGTTTTGA GGAGAAGAAG
    ACCACCAGAG GCCAAAACCT GCCGTCCTTC CCTCCCATCA GCCTTCTTTA CACCAAGAAA
    CAAGTTTTCC AGCTGCGAGC CCACATGTAC CAGGCCAGGA GTTTATTTGC TGCTGATAGT
    AGTGGCCTTT CAGATCCCTT TGCCCGAGTT TTCTTCACTT CCCAGAGCCA ATGCACTGAG
    GTTCTCAATG AGACCCTGTG CCCAACCTGG GACCAGCTGC TGGTTTTTGA CAACGTGGAG
    CTGTACGGAG AAGCCCATGA GATGCGGGAC GATCCTCCTA TAATTGTCAT TGAGATCTAT
    GATCAAGACA CAGTGGGCAA AGCTGACTTC ATGGGCCGCA CCTTTGCCAA ACCAGTGGTG
    AAAATGTCAG ATGAGCAGTA CTGCCCCCCA CGCTTCCCCC CACAGCTGGA ATACTACCAG
    ATCTACCGGG GCAACTCTAC AGCAGGAGAC CTTCTGGCTG CCTTCGAGCT GCTCCAGATT
    GGCTCTGGGG GCAAATCAGA CCTGCCTCCA ATTGATGGCC CCACAGACAT AGACCGAGGC
    CCCATCCTGC CGGTGCCACT GGGGATTCGG CCGGTGCTGA GTAAATACAG AGTGGAGATC
    TTGTTTTGGG GACTCAGGGA CCTGAAGAGA GTGAACCTGG CCCAGGTGGA CCGGCCCCGT
    GTGGACATTG AGTGTGCTGG GAAGGGAGTT CAGTCAGCTC TCATCCAGAA CTACAAGAAA
    AACCCAAACT TCAGCACTTT GGTCAAGTGG TTTGAAGTGG ATCTCCCAGA GAATGAGCTG
    CTCCACCCAC CTCTGAACAT CCGAGTGGTG GACTGCCGGG CTTTTGGGCG CTACACCTTG
    GTGGGCTCCC ATACCGTCAG CTCCCTCCGA AAGTTCATCT ACCGCCCACC AGATAAGAAA
    GCCCAGCACT GGAATATGAC AGCTAAACAG CTCAAAGGCT ACTTGGCAAT GGCCAACGGG
    GCCCCTCGCT CTCGCCCCAC AGGGGAGATC ATTGTCAACA TGGAACCTGA GGTTCCTATC
    AAGAAGATGG AGACCATGGT GAAGCTGGAA GCTAACTCCG ACGCAGTGGT GAAGGTGGAT
    GTGTCTGAAG AAGAGAAGGA GAAAAAGAAA AAGAAGAAGA AAGGAGGTGG AGGAGGAGGA
    GGAGGAGAAG AGATAGAAGA AGAGGAGCCA GATGAGAGCA TGCTGGACTG GTGGTCCAAG
    TATTTTGCTT CAATTGAGAC TATGAAAGAG CAACTCCGGC AGCAGGAAGC AGCTGCAGCA
    GAAGCAGAGG AGAAGGAAGA GATGGAGATT GCAGAGGAAA TCAAACTTGA TGACTCTCCC
    ATGAAAGGCT TCAAGGGCCA GGCAAAGAAC AAGGAGAAGT CCAAGGCACC CAAAGATGAC
    AAGAAGAAGA AGCAGCAGTC AGCCCCTGAG CTCCCTGAGA AGAAGAACAA GCAGAAGATT
    GATGAACTGA AGGTCTTCAA CAAAGAGCTG GAAGCAGAGT TTGATAACTT TGAGGACTGG
    CTGCACACCT TCAACCTGCT CCGTGGGAAG ATTGGGGACA ATGATGACAA TGCAACAGAA
    GAAGAGCGGA TAGTGGGAAG GTTCAAAGGC TCCATGTGTG TCTACAAAGT GCCTCTCCCT
    GATGATATCA CCAAGGAGGC AGGATATGAC CCCACCTTTG GCATGTTCCA GGGGATTCCC
    AGCAACGACC CCATCAATGT GCTGGTCCGA GTCTATATTG TGAGGGCCAC AGACCTCCAC
    CCAGCTGACA TCAATGGGAA AGCTGACCCT TATATTGCCA TCAAGCTGGG GAAGACAGAC
    ATCAAAGACA AGGAGAACTA TATCTCCAAG CAGCTGAATC CTGTCTTCGG AAAGTCCTTT
    GACATTGAGG CCACCTTCCC CATGGAGTCC ATGCTGACAG TGGCAGTGTA TGACTGGGAC
    TTGGTGGGGA CAGATGACCT CATTGGGGAG ACCAAGATTG ACCTGGAGAA CCGCTACTAC
    AGCAAGCACC GAGCTACGTG TGGGGTGTCA CAGACTTACT CCATACATGG GTATAACATC
    TGGCGTGACC CCATGAAGCC CTCCCAGATC CTGTCCAAGC TGTGCAAAGA GGGCAAGGTG
    GATGGGCCAC ACTTCGGGCC AGGAGGAAGA GTGAAAGTTG CCAACAGGGT GTACACTGGT
    CCCACGGAGA TTGAGGATGA AAATGGTCAG AAGAAACAGA CAGATGAACA CTTGGCCCTG
    ACCGTGTTGC GCCACTGGGA GGACGTCCCA CGGGCAGGCT GCAAGCTGGT CCCTGAGCAC
    GTGGAGACGC GCCCGCTGCT GAACCCTGAC AAGCCGGGCA TTGAGCAGGG TCGCCTGGAG
    ATGTGGGTGG ATATGTTCCC CATGGATATG CCGGCACCTG GTCCTGCCAT TGATATCTCC
    CCCAGGAAAC CAAAGAAATA TGAGCTCAGA GTGATAGTGT GGAACACAGA TGAGGTCATC
    CTGGAAGATG ATGACTACTT CACTGGCGAG AAATCCAGTG ACATTTTTGT CAGGGGGTGG
    CTGAAGGGTC AGCAGGAGGA CAAGCAAGAC ACAGACGTCC ATTACCACTC GCTCACCGGC
    GAAGGCAACT TCAACTGGCG TTACATCTTC CCCTTTGACT ACCTGATGGC AGAGGAGAAG
    ATCGTCATCT CCAAGAAAGA GTCCATGTTC TCCTGGGATG AGACTGAGTA CAAGATCCCA
    GCTCGCCTCA CCCTGCAGGT CTGGGATGCC GACCACTTCT CAGCCGATGA TTTCCTTGGG
    GCCATCGAGT TGGACCTGAA CCGCTTTCCG CGGGGAGCCA AGACATCCAA ACAGTGCAGT
    CTGGAGATGG TGACAAACGA GGCAGAGCTG CCCATGGTCT CAATCTTCAA GCAAAAGCGG
    GTGAAGGGCT GGTGGCCATT TGTGGCCAGG GATGAGAATG ACGAGCTGGA GATCACCGGG
    AAAGTCGAGG CTGAACTACA CCTCCTGACT GCAGAGGAGG CAGAGAAATC TCCTGCTGGG
    CTGGCTCGCA ATGAACCCGA CCCACTGGAG AAACCCAACC GTCCAGACAC GTCTTTCATC
    TGGTTCCTCA ACCCTCTCAA GTCCATCAAA TACCTCATCT GCACCCGCTA CAAGTGGCTC
    ATCATCAAAA TCGTGCTGGC CCTGTTGCTC CTCATCATGG TGGCCCTCTT CCTCTACAGC
    ATGCCAGGCT ACATGGTCAA GAAGCTCCTG GGGGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34480
    Clone ID Related Accession (Same CDS sequence) XM_015285207.1 , XM_015285207.2
    Accession Version XM_015285207.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTA CCTGCCTCAC GGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140693.1
    CDS155..5461
    Translation

    Target ORF information:

    RefSeq Version XM_015285207.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTA CCTGCCTCAC GGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34480
    Clone ID Related Accession (Same CDS sequence) XM_015285207.1 , XM_015285207.2
    Accession Version XM_015285207.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTA CCTGCCTCAC GGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140693.1
    CDS152..5458
    Translation

    Target ORF information:

    RefSeq Version XM_015285207.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285207.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTA CCTGCCTCAC GGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07078
    Clone ID Related Accession (Same CDS sequence) XM_004935859.2 , XM_004935859.3
    Accession Version XM_004935859.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935916.1
    CDS152..6124
    Translation

    Target ORF information:

    RefSeq Version XM_004935859.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935859.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07078
    Clone ID Related Accession (Same CDS sequence) XM_004935859.2 , XM_004935859.3
    Accession Version XM_004935859.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5973bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935916.1
    CDS1..5973
    Translation

    Target ORF information:

    RefSeq Version XM_004935859.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935859.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34472
    Clone ID Related Accession (Same CDS sequence) XM_015285198.2 , XM_015285198.1
    Accession Version XM_015285198.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140684.1
    CDS149..6469
    Translation

    Target ORF information:

    RefSeq Version XM_015285198.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285198.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34472
    Clone ID Related Accession (Same CDS sequence) XM_015285198.2 , XM_015285198.1
    Accession Version XM_015285198.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140684.1
    CDS153..6473
    Translation

    Target ORF information:

    RefSeq Version XM_015285198.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34473
    Clone ID Related Accession (Same CDS sequence) XM_015285199.2 , XM_015285199.1
    Accession Version XM_015285199.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285199.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140685.1
    CDS150..6440
    Translation

    Target ORF information:

    RefSeq Version XM_015285199.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285199.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34473
    Clone ID Related Accession (Same CDS sequence) XM_015285199.2 , XM_015285199.1
    Accession Version XM_015285199.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140685.1
    CDS153..6443
    Translation

    Target ORF information:

    RefSeq Version XM_015285199.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285199.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGCTAAAC AGCTCAAAGG CTACTTGGCA ATGGCCAACG GGGCCCCTCG CTCTCGCCCC
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34474
    Clone ID Related Accession (Same CDS sequence) XM_015285200.2 , XM_015285200.1
    Accession Version XM_015285200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC
    AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG
    CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG
    GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC
    ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT
    GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC
    ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG
    GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC
    TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT
    GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC
    GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT
    GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG
    GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT
    GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC
    ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG
    AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT
    GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG
    CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA
    GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC
    TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT
    GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG
    GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC
    ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT
    GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC
    ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT
    GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT
    GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT
    GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC
    TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG
    GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG
    GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG
    CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC
    AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG
    CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT
    ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA
    GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT
    ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG
    GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC
    GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC
    AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC
    AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT
    TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC
    CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC
    AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC
    GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA
    GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG
    TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC
    CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT
    GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC
    CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT
    TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC
    ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA
    AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC
    CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC
    TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG
    CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT
    CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG
    ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT
    GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA
    GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT
    GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA
    GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140686.1
    CDS149..6421
    Translation

    Target ORF information:

    RefSeq Version XM_015285200.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC
    AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG
    CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG
    GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC
    ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT
    GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC
    ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG
    GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC
    TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT
    GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC
    GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT
    GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG
    GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT
    GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC
    ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG
    AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT
    GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG
    CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA
    GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC
    TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT
    GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG
    GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC
    ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT
    GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC
    ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT
    GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT
    GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT
    GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC
    TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG
    GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG
    GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG
    CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC
    AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG
    CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT
    ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA
    GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT
    ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG
    GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC
    GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC
    AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC
    AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT
    TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC
    CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC
    AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC
    GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA
    GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG
    TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC
    CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT
    GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC
    CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT
    TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC
    ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA
    AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC
    CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC
    TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG
    CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT
    CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG
    ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT
    GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA
    GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT
    GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA
    GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34474
    Clone ID Related Accession (Same CDS sequence) XM_015285200.2 , XM_015285200.1
    Accession Version XM_015285200.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6273bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC
    AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG
    CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG
    GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC
    ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT
    GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC
    ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG
    GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC
    TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT
    GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC
    GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT
    GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG
    GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT
    GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC
    ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG
    AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT
    GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG
    CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA
    GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC
    TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT
    GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG
    GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC
    ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT
    GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC
    ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT
    GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT
    GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT
    GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC
    TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG
    GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG
    GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG
    CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC
    AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG
    CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT
    ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA
    GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT
    ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG
    GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC
    GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC
    AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC
    AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT
    TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC
    CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC
    AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC
    GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA
    GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG
    TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC
    CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT
    GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC
    CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT
    TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC
    ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA
    AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC
    CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC
    TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG
    CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT
    CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG
    ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT
    GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA
    GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT
    GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA
    GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140686.1
    CDS153..6425
    Translation

    Target ORF information:

    RefSeq Version XM_015285200.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285200.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGC
    AAAGGCAAAG AGAAGACAAA GGAGCACAAA GCTGGGAAAG GAGTTTTTTC AGCCATGAAG
    CTTGGCAAGG CACGTCCCAC AAAAGATGAC CATAGGAAGC AAGATGAACC TGCTGTTCTG
    GAGGCAGAAG ACCTGGACCG AAAAGTCATG AGACTGGGAG GGGGGCTGGA CCCGGACACC
    ATCTCCTTGG CCTCTGTCAC AGCTGTCACC ACCAATGTCT CAAACAAGAG GTCCAAACCT
    GACATCAAAA TGGAGCCAAG TGCTGGGAGG CCGATGGATT ACCAGGTCAG CATCACTGTC
    ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC ATGGACCCCG TGGTCTGCGT GGAGGTTGGG
    GAGGAGAAGA AATACACATC CATGAAGGAA TCGACCAACT GCCCTTACTA CAATGAGTAC
    TTTGTCTTCG ATTTCCACGT GCCCCCGGAT GTCATGTTTG ATAAGATCAT CAAGCTGTCT
    GTGATCCACT CAAAAAACCT CTTGCGCAGT GGGACTTTGG TGGGCTCCTT CAAGATGGAC
    GTTGGGACAG TTTACACCCA GCCTGAGCAC CAGTTCTACC ACAAATGGGC CATCCTTTCT
    GACCCCGAGG ACCTCACTGC TGGGCTGAAA GGTTACCTGA AGTGTGACAT CGCTGTTGTG
    GGGAAAGGGG ACAACATCAA GACACCCCAC AAAGCCAATG AGACAGAGGA GGATGATATT
    GAAGGGAACC TCCTCCTACC CGATGGGGTT CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC
    ATCAAGATCT ATCGAGCAGA GGGCCTGCCC CGTATGAACA CCAGCATCAT GGCCAATGTG
    AAGAAGGCTC TCATTGGAGA GAATAAGGAC TTGGTAGACC CCTACGTGCA AGTGGCATTT
    GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA AAAAGCAGCT ATGAGCCTCT CTGGAATGAG
    CAAATCATCT TCACAGAAAT GTTCCCTCCA TTGTGCAAGC GGATAAAGAT CCAGATCCGA
    GACTCGGACA AAGTCAATGA TGTGGCCATT GGTACCCATT TCATTGATTT GAGGAAGATC
    TCTAATGAAG GAGACAAAGG CTACCTGCCC ACCTTTGGCC CTGCATGGGT GAACATGTAT
    GGCTCCACTC GGAACTACAC CCTGATGGAT GAACACCAAG AGCTGAATGA GGGGCTGGGG
    GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG ATGAGCCTTG CGGTTGAGAT CTTGGATACC
    ACCAACCCAG AGATCAACAG CTCCACTGAG GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT
    GATAACTGCA CTGGAAAGAT GGAGGAGTTC TTTCTTTTTG GAGCCTTCCT GGAAGCTACC
    ATGATCGACA GGAAGATCGG TGACAAACCC ATCAACTTTG AGGTTACAAT AGGCAACTAT
    GGGAACCAGA TTGATGGCAT GAACAAACCT GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT
    GGGGACGAGG AGGAGTCCGA GCTGCTACAC AACTCAAGTG AGGATGAAGC TGATGAGGAT
    GGAGAGATGG TCTCTGTCTC CTCGTCTCAG CCCATGAAGC CCCTGGTTAC AGACAGGAAC
    TACTTCCACC TGCCGTACTT TGAGAAGAAG CCCTGCATCT ACATCAAGAG CTGGTGGCAG
    GATCAGCGCC GCAGGCTGTA TAACGCCAAC ATCATGGACA AGATTGCAGA CAAGCTGGAG
    GAAGGGCTCA ATGATGTGCA GGAAATGATC AAGACAGAGA AACCACATCC AGAGCGCCGG
    CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC
    AAGGACCAGC ACCACTCTTC CCGCACGAGG TTGGACCGAG AGAGGCTCAA GTCCTGCATG
    CGGGAGCTGG AGAACATGGG CCAGCAAGCC ACGACTCTGA GGTCGCAGGT GAAGAAGAAT
    ACCATGAAAG ACAAGCTGAA GCAGGTGCAG AACTTCCTGC AGAAACTCCG TTTCTTGGCA
    GATGAGCCCC AGCACACCAT CCCTGACATC TTCATCTGGA TGATGAGCAA TAACAAGCGT
    ATTGCCTATG CCCGTGTCCC TTCCAAGGAC ATCCTCTATT CCATTGTGGA TGAGGAGATG
    GGCAAGGACT GCGCCAAAGT GAAGACTGTC TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC
    GGACCTGCCG GCTGGACAGT TCAGGCCAAG ATGGAGATTT ACTTGTGGCT GGGCCTCAAC
    AAGCAGCGCA AAGACTTCCT GAGTGGCTTG CCCTGTGGTT TTGAGGAGAA GAAGACCACC
    AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC ATCAGCCTTC TTTACACCAA GAAACAAGTT
    TTCCAGCTGC GAGCCCACAT GTACCAGGCC AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC
    CTTTCAGATC CCTTTGCCCG AGTTTTCTTC ACTTCCCAGA GCCAATGCAC TGAGGTTCTC
    AATGAGACCC TGTGCCCAAC CTGGGACCAG CTGCTGGTTT TTGACAACGT GGAGCTGTAC
    GGAGAAGCCC ATGAGATGCG GGACGATCCT CCTATAATTG TCATTGAGAT CTATGATCAA
    GACACAGTGG GCAAAGCTGA CTTCATGGGC CGCACCTTTG CCAAACCAGT GGTGAAAATG
    TCAGATGAGC AGTACTGCCC CCCACGCTTC CCCCCACAGC TGGAATACTA CCAGATCTAC
    CGGGGCAACT CTACAGCAGG AGACCTTCTG GCTGCCTTCG AGCTGCTCCA GATTGGCTCT
    GGGGGCAAAT CAGACCTGCC TCCAATTGAT GGCCCCACAG ACATAGACCG AGGCCCCATC
    CTGCCGGTGC CACTGGGGAT TCGGCCGGTG CTGAGTAAAT ACAGAGTGGA GATCTTGTTT
    TGGGGACTCA GGGACCTGAA GAGAGTGAAC CTGGCCCAGG TGGACCGGCC CCGTGTGGAC
    ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA GCTCTCATCC AGAACTACAA GAAAAACCCA
    AACTTCAGCA CTTTGGTCAA GTGGTTTGAA GTGGATCTCC CAGAGAATGA GCTGCTCCAC
    CCACCTCTGA ACATCCGAGT GGTGGACTGC CGGGCTTTTG GGCGCTACAC CTTGGTGGGC
    TCCCATACCG TCAGCTCCCT CCGAAAGTTC ATCTACCGCC CACCAGATAA GAAAGCCCAG
    CACTGGAATA TGACAGCTAA ACAGCTCAAA GGCTACTTGG CAATGGCCAA CGGGGCCCCT
    CGCTCTCGCC CCACAGGGGA GATCATTGTC AACATGGAAC CTGAGGTTCC TATCAAGAAG
    ATGGAGACCA TGGTGAAGCT GGAAGCTAAC TCCGACGCAG TGGTGAAGGT GGATGTGTCT
    GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA
    GAAGAGATAG AAGAAGAGGA GCCAGATGAG AGCATGCTGG ACTGGTGGTC CAAGTATTTT
    GCTTCAATTG AGACTATGAA AGAGCAACTC CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA
    GAGGAGAAGG AAGAGATGGA GATTGCAGAG GAAATCAAAC TTGATGACTC TCCCATGAAA
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34475
    Clone ID Related Accession (Same CDS sequence) XM_015285201.1 , XM_015285201.2
    Accession Version XM_015285201.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140687.1
    CDS153..6413
    Translation

    Target ORF information:

    RefSeq Version XM_015285201.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285201.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34475
    Clone ID Related Accession (Same CDS sequence) XM_015285201.1 , XM_015285201.2
    Accession Version XM_015285201.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285201.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140687.1
    CDS149..6409
    Translation

    Target ORF information:

    RefSeq Version XM_015285201.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285201.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGA AATCAAACTT GATGACTCTC CCATGAAAGG CTTCAAGGGC
    CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA CCCAAAGATG ACAAGAAGAA GAAGCAGCAG
    TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC AAGCAGAAGA TTGATGAACT GAAGGTCTTC
    AACAAAGAGC TGGAAGCAGA GTTTGATAAC TTTGAGGACT GGCTGCACAC CTTCAACCTG
    CTCCGTGGGA AGATTGGGGA CAATGATGAC AATGCAACAG AAGAAGAGCG GATAGTGGGA
    AGGTTCAAAG GCTCCATGTG TGTCTACAAA GTGCCTCTCC CTGATGATAT CACCAAGGAG
    GCAGGATATG ACCCCACCTT TGGCATGTTC CAGGGGATTC CCAGCAACGA CCCCATCAAT
    GTGCTGGTCC GAGTCTATAT TGTGAGGGCC ACAGACCTCC ACCCAGCTGA CATCAATGGG
    AAAGCTGACC CTTATATTGC CATCAAGCTG GGGAAGACAG ACATCAAAGA CAAGGAGAAC
    TATATCTCCA AGCAGCTGAA TCCTGTCTTC GGAAAGTCCT TTGACATTGA GGCCACCTTC
    CCCATGGAGT CCATGCTGAC AGTGGCAGTG TATGACTGGG ACTTGGTGGG GACAGATGAC
    CTCATTGGGG AGACCAAGAT TGACCTGGAG AACCGCTACT ACAGCAAGCA CCGAGCTACG
    TGTGGGGTGT CACAGACTTA CTCCATACAT GGGTATAACA TCTGGCGTGA CCCCATGAAG
    CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA GAGGGCAAGG TGGATGGGCC ACACTTCGGG
    CCAGGAGGAA GAGTGAAAGT TGCCAACAGG GTGTACACTG GTCCCACGGA GATTGAGGAT
    GAAAATGGTC AGAAGAAACA GACAGATGAA CACTTGGCCC TGACCGTGTT GCGCCACTGG
    GAGGACGTCC CACGGGCAGG CTGCAAGCTG GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG
    CTGAACCCTG ACAAGCCGGG CATTGAGCAG GGTCGCCTGG AGATGTGGGT GGATATGTTC
    CCCATGGATA TGCCGGCACC TGGTCCTGCC ATTGATATCT CCCCCAGGAA ACCAAAGAAA
    TATGAGCTCA GAGTGATAGT GTGGAACACA GATGAGGTCA TCCTGGAAGA TGATGACTAC
    TTCACTGGCG AGAAATCCAG TGACATTTTT GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG
    GACAAGCAAG ACACAGACGT CCATTACCAC TCGCTCACCG GCGAAGGCAA CTTCAACTGG
    CGTTACATCT TCCCCTTTGA CTACCTGATG GCAGAGGAGA AGATCGTCAT CTCCAAGAAA
    GAGTCCATGT TCTCCTGGGA TGAGACTGAG TACAAGATCC CAGCTCGCCT CACCCTGCAG
    GTCTGGGATG CCGACCACTT CTCAGCCGAT GATTTCCTTG GGGCCATCGA GTTGGACCTG
    AACCGCTTTC CGCGGGGAGC CAAGACATCC AAACAGTGCA GTCTGGAGAT GGTGACAAAC
    GAGGCAGAGC TGCCCATGGT CTCAATCTTC AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA
    TTTGTGGCCA GGGATGAGAA TGACGAGCTG GAGATCACCG GGAAAGTCGA GGCTGAACTA
    CACCTCCTGA CTGCAGAGGA GGCAGAGAAA TCTCCTGCTG GGCTGGCTCG CAATGAACCC
    GACCCACTGG AGAAACCCAA CCGTCCAGAC ACGTCTTTCA TCTGGTTCCT CAACCCTCTC
    AAGTCCATCA AATACCTCAT CTGCACCCGC TACAAGTGGC TCATCATCAA AATCGTGCTG
    GCCCTGTTGC TCCTCATCAT GGTGGCCCTC TTCCTCTACA GCATGCCAGG CTACATGGTC
    AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34476
    Clone ID Related Accession (Same CDS sequence) XM_015285202.1 , XM_015285202.2
    Accession Version XM_015285202.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC
    CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG
    TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA
    CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA
    GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG
    CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA
    GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC
    TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT
    TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC
    GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC
    TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT
    GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG
    GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC
    CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG
    AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT
    GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG
    AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC
    ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC
    CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC
    TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG
    CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT
    TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC
    CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA
    GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT
    GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG
    GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT
    GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT
    GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG
    AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT
    GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG
    CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC
    TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC
    AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG
    AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG
    CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA
    GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG
    AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG
    CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG
    ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT
    TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG
    CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT
    TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT
    TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT
    CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA
    TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG
    AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT
    TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT
    GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT
    GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG
    CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC
    GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA
    GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA
    TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG
    GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC
    CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC
    CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT
    GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC
    CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG
    GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA
    CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA
    GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA
    GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG
    GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG
    GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA
    CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG
    GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG
    AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT
    AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT
    GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC
    AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG
    TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG
    GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG
    CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC
    TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA
    GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG
    GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA
    CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC
    AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC
    AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT
    GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG
    CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG
    CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT
    GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC
    ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT
    TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC
    CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG
    ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT
    GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC
    GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA
    TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC
    TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG
    CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG
    AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA
    GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC
    CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC
    CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140688.1
    CDS153..6386
    Translation

    Target ORF information:

    RefSeq Version XM_015285202.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC
    CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG
    TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA
    CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA
    GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG
    CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA
    GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC
    TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT
    TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC
    GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC
    TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT
    GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG
    GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC
    CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG
    AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT
    GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG
    AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC
    ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC
    CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC
    TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG
    CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT
    TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC
    CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA
    GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT
    GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG
    GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT
    GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT
    GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG
    AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT
    GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG
    CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC
    TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC
    AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG
    AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG
    CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA
    GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG
    AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG
    CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG
    ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT
    TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG
    CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT
    TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT
    TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT
    CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA
    TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG
    AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT
    TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT
    GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT
    GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG
    CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC
    GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA
    GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA
    TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG
    GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC
    CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC
    CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT
    GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC
    CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG
    GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA
    CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA
    GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA
    GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG
    GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG
    GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA
    CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG
    GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG
    AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT
    AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT
    GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC
    AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG
    TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG
    GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG
    CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC
    TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA
    GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG
    GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA
    CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC
    AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC
    AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT
    GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG
    CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG
    CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT
    GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC
    ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT
    TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC
    CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG
    ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT
    GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC
    GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA
    TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC
    TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG
    CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG
    AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA
    GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC
    CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC
    CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34476
    Clone ID Related Accession (Same CDS sequence) XM_015285202.1 , XM_015285202.2
    Accession Version XM_015285202.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285202.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC
    CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG
    TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA
    CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA
    GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG
    CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA
    GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC
    TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT
    TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC
    GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC
    TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT
    GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG
    GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC
    CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG
    AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT
    GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG
    AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC
    ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC
    CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC
    TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG
    CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT
    TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC
    CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA
    GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT
    GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG
    GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT
    GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT
    GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG
    AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT
    GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG
    CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC
    TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC
    AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG
    AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG
    CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA
    GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG
    AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG
    CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG
    ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT
    TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG
    CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT
    TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT
    TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT
    CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA
    TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG
    AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT
    TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT
    GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT
    GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG
    CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC
    GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA
    GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA
    TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG
    GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC
    CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC
    CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT
    GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC
    CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG
    GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA
    CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA
    GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA
    GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG
    GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG
    GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA
    CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG
    GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG
    AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT
    AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT
    GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC
    AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG
    TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG
    GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG
    CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC
    TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA
    GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG
    GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA
    CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC
    AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC
    AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT
    GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG
    CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG
    CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT
    GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC
    ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT
    TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC
    CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG
    ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT
    GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC
    GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA
    TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC
    TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG
    CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG
    AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA
    GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC
    CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC
    CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140688.1
    CDS149..6382
    Translation

    Target ORF information:

    RefSeq Version XM_015285202.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285202.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGC
    CTTGGAGACG ATGCGGAAGA AGAGCAGCAC ACTAGGATTA AGTTTAACGA CGGATACAAG
    TCAGTAGCAA GCGAAGACCC TGAAGACACC AGAGCTAAGG CTAAGGGGAA AGAGAAAAGA
    CACCTCATGT TGCCATTCAG CAAAGGCAAA GAGAAGACAA AGGAGCACAA AGCTGGGAAA
    GGAGTTTTTT CAGCCATGAA GCTTGGCAAG GCACGTCCCA CAAAAGATGA CCATAGGAAG
    CAAGATGAAC CTGCTGTTCT GGAGGCAGAA GACCTGGACC GAAAAGTCAT GAGACTGGGA
    GGGGGGCTGG ACCCGGACAC CATCTCCTTG GCCTCTGTCA CAGCTGTCAC CACCAATGTC
    TCAAACAAGA GGTCCAAACC TGACATCAAA ATGGAGCCAA GTGCTGGGAG GCCGATGGAT
    TACCAGGTCA GCATCACTGT CATCGAGGCC CGGCAGCTGG TTGGGCTCAA CATGGACCCC
    GTGGTCTGCG TGGAGGTTGG GGAGGAGAAG AAATACACAT CCATGAAGGA ATCGACCAAC
    TGCCCTTACT ACAATGAGTA CTTTGTCTTC GATTTCCACG TGCCCCCGGA TGTCATGTTT
    GATAAGATCA TCAAGCTGTC TGTGATCCAC TCAAAAAACC TCTTGCGCAG TGGGACTTTG
    GTGGGCTCCT TCAAGATGGA CGTTGGGACA GTTTACACCC AGCCTGAGCA CCAGTTCTAC
    CACAAATGGG CCATCCTTTC TGACCCCGAG GACCTCACTG CTGGGCTGAA AGGTTACCTG
    AAGTGTGACA TCGCTGTTGT GGGGAAAGGG GACAACATCA AGACACCCCA CAAAGCCAAT
    GAGACAGAGG AGGATGATAT TGAAGGGAAC CTCCTCCTAC CCGATGGGGT TCCTCCAGAG
    AGGCAGTGGG CTCGTTTCTA CATCAAGATC TATCGAGCAG AGGGCCTGCC CCGTATGAAC
    ACCAGCATCA TGGCCAATGT GAAGAAGGCT CTCATTGGAG AGAATAAGGA CTTGGTAGAC
    CCCTACGTGC AAGTGGCATT TGCAGGGCAG AAGGGGAAGA CATCAGTGCA AAAAAGCAGC
    TATGAGCCTC TCTGGAATGA GCAAATCATC TTCACAGAAA TGTTCCCTCC ATTGTGCAAG
    CGGATAAAGA TCCAGATCCG AGACTCGGAC AAAGTCAATG ATGTGGCCAT TGGTACCCAT
    TTCATTGATT TGAGGAAGAT CTCTAATGAA GGAGACAAAG GCTACCTGCC CACCTTTGGC
    CCTGCATGGG TGAACATGTA TGGCTCCACT CGGAACTACA CCCTGATGGA TGAACACCAA
    GAGCTGAATG AGGGGCTGGG GGAAGGCGTC TCCTTCCGGG CCAGGCTGCT GATGAGCCTT
    GCGGTTGAGA TCTTGGATAC CACCAACCCA GAGATCAACA GCTCCACTGA GGTGCAAGTG
    GAGCAGGCCA CACCAGTTGC TGATAACTGC ACTGGAAAGA TGGAGGAGTT CTTTCTTTTT
    GGAGCCTTCC TGGAAGCTAC CATGATCGAC AGGAAGATCG GTGACAAACC CATCAACTTT
    GAGGTTACAA TAGGCAACTA TGGGAACCAG ATTGATGGCA TGAACAAACC TGTCCTGCGG
    AGGAAGAAGG AGGGAGGGGA TGGGGACGAG GAGGAGTCCG AGCTGCTACA CAACTCAAGT
    GAGGATGAAG CTGATGAGGA TGGAGAGATG GTCTCTGTCT CCTCGTCTCA GCCCATGAAG
    CCCCTGGTTA CAGACAGGAA CTACTTCCAC CTGCCGTACT TTGAGAAGAA GCCCTGCATC
    TACATCAAGA GCTGGTGGCA GGATCAGCGC CGCAGGCTGT ATAACGCCAA CATCATGGAC
    AAGATTGCAG ACAAGCTGGA GGAAGGGCTC AATGATGTGC AGGAAATGAT CAAGACAGAG
    AAACCACATC CAGAGCGCCG GCTCCGAGGG GTCCTGGAGG AGCTGAGCAG CGGGTGCTTG
    CGCTTTGTGA CGCTGGCTGA CAAGGACCAG CACCACTCTT CCCGCACGAG GTTGGACCGA
    GAGAGGCTCA AGTCCTGCAT GCGGGAGCTG GAGAACATGG GCCAGCAAGC CACGACTCTG
    AGGTCGCAGG TGAAGAAGAA TACCATGAAA GACAAGCTGA AGCAGGTGCA GAACTTCCTG
    CAGAAACTCC GTTTCTTGGC AGATGAGCCC CAGCACACCA TCCCTGACAT CTTCATCTGG
    ATGATGAGCA ATAACAAGCG TATTGCCTAT GCCCGTGTCC CTTCCAAGGA CATCCTCTAT
    TCCATTGTGG ATGAGGAGAT GGGCAAGGAC TGCGCCAAAG TGAAGACTGT CTTTCTCAAG
    CTGCCTGGGA AGAGGGGTTT CGGACCTGCC GGCTGGACAG TTCAGGCCAA GATGGAGATT
    TACTTGTGGC TGGGCCTCAA CAAGCAGCGC AAAGACTTCC TGAGTGGCTT GCCCTGTGGT
    TTTGAGGAGA AGAAGACCAC CAGAGGCCAA AACCTGCCGT CCTTCCCTCC CATCAGCCTT
    CTTTACACCA AGAAACAAGT TTTCCAGCTG CGAGCCCACA TGTACCAGGC CAGGAGTTTA
    TTTGCTGCTG ATAGTAGTGG CCTTTCAGAT CCCTTTGCCC GAGTTTTCTT CACTTCCCAG
    AGCCAATGCA CTGAGGTTCT CAATGAGACC CTGTGCCCAA CCTGGGACCA GCTGCTGGTT
    TTTGACAACG TGGAGCTGTA CGGAGAAGCC CATGAGATGC GGGACGATCC TCCTATAATT
    GTCATTGAGA TCTATGATCA AGACACAGTG GGCAAAGCTG ACTTCATGGG CCGCACCTTT
    GCCAAACCAG TGGTGAAAAT GTCAGATGAG CAGTACTGCC CCCCACGCTT CCCCCCACAG
    CTGGAATACT ACCAGATCTA CCGGGGCAAC TCTACAGCAG GAGACCTTCT GGCTGCCTTC
    GAGCTGCTCC AGATTGGCTC TGGGGGCAAA TCAGACCTGC CTCCAATTGA TGGCCCCACA
    GACATAGACC GAGGCCCCAT CCTGCCGGTG CCACTGGGGA TTCGGCCGGT GCTGAGTAAA
    TACAGAGTGG AGATCTTGTT TTGGGGACTC AGGGACCTGA AGAGAGTGAA CCTGGCCCAG
    GTGGACCGGC CCCGTGTGGA CATTGAGTGT GCTGGGAAGG GAGTTCAGTC AGCTCTCATC
    CAGAACTACA AGAAAAACCC AAACTTCAGC ACTTTGGTCA AGTGGTTTGA AGTGGATCTC
    CCAGAGAATG AGCTGCTCCA CCCACCTCTG AACATCCGAG TGGTGGACTG CCGGGCTTTT
    GGGCGCTACA CCTTGGTGGG CTCCCATACC GTCAGCTCCC TCCGAAAGTT CATCTACCGC
    CCACCAGATA AGAAAGCCCA GCACTGGAAT ATGACAGCTA AACAGCTCAA AGGCTACTTG
    GCAATGGCCA ACGGGGCCCC TCGCTCTCGC CCCACAGGGG AGATCATTGT CAACATGGAA
    CCTGAGGTTC CTATCAAGAA GATGGAGACC ATGGTGAAGC TGGAAGCTAA CTCCGACGCA
    GTGGTGAAGG TGGATGTGTC TGAAGAAGAG AAGGAGAAAA AGAAAAAGAA GAAGAAAGGA
    GGTGGAGGAG GAGGAGGAGG AGAAGAGATA GAAGAAGAGG AGCCAGATGA GAGCATGCTG
    GACTGGTGGT CCAAGTATTT TGCTTCAATT GAGACTATGA AAGAGCAACT CCGGCAGCAG
    GAAGCAGCTG CAGCAGAAGC AGAGGAGAAG GAAGAGATGG AGATTGCAGA GGAAATCAAA
    CTTGATGACT CTCCCATGAA AGGCTTCAAG GGCCAGGCAA AGAACAAGGA GAAGTCCAAG
    GCACCCAAAG ATGACAAGAA GAAGAAGCAG CAGTCAGCCC CTGAGCTCCC TGAGAAGAAG
    AACAAGCAGA AGATTGATGA ACTGAAGGTC TTCAACAAAG AGCTGGAAGC AGAGTTTGAT
    AACTTTGAGG ACTGGCTGCA CACCTTCAAC CTGCTCCGTG GGAAGATTGG GGACAATGAT
    GACAATGCAA CAGAAGAAGA GCGGATAGTG GGAAGGTTCA AAGGCTCCAT GTGTGTCTAC
    AAAGTGCCTC TCCCTGATGA TATCACCAAG GAGGCAGGAT ATGACCCCAC CTTTGGCATG
    TTCCAGGGGA TTCCCAGCAA CGACCCCATC AATGTGCTGG TCCGAGTCTA TATTGTGAGG
    GCCACAGACC TCCACCCAGC TGACATCAAT GGGAAAGCTG ACCCTTATAT TGCCATCAAG
    CTGGGGAAGA CAGACATCAA AGACAAGGAG AACTATATCT CCAAGCAGCT GAATCCTGTC
    TTCGGAAAGT CCTTTGACAT TGAGGCCACC TTCCCCATGG AGTCCATGCT GACAGTGGCA
    GTGTATGACT GGGACTTGGT GGGGACAGAT GACCTCATTG GGGAGACCAA GATTGACCTG
    GAGAACCGCT ACTACAGCAA GCACCGAGCT ACGTGTGGGG TGTCACAGAC TTACTCCATA
    CATGGGTATA ACATCTGGCG TGACCCCATG AAGCCCTCCC AGATCCTGTC CAAGCTGTGC
    AAAGAGGGCA AGGTGGATGG GCCACACTTC GGGCCAGGAG GAAGAGTGAA AGTTGCCAAC
    AGGGTGTACA CTGGTCCCAC GGAGATTGAG GATGAAAATG GTCAGAAGAA ACAGACAGAT
    GAACACTTGG CCCTGACCGT GTTGCGCCAC TGGGAGGACG TCCCACGGGC AGGCTGCAAG
    CTGGTCCCTG AGCACGTGGA GACGCGCCCG CTGCTGAACC CTGACAAGCC GGGCATTGAG
    CAGGGTCGCC TGGAGATGTG GGTGGATATG TTCCCCATGG ATATGCCGGC ACCTGGTCCT
    GCCATTGATA TCTCCCCCAG GAAACCAAAG AAATATGAGC TCAGAGTGAT AGTGTGGAAC
    ACAGATGAGG TCATCCTGGA AGATGATGAC TACTTCACTG GCGAGAAATC CAGTGACATT
    TTTGTCAGGG GGTGGCTGAA GGGTCAGCAG GAGGACAAGC AAGACACAGA CGTCCATTAC
    CACTCGCTCA CCGGCGAAGG CAACTTCAAC TGGCGTTACA TCTTCCCCTT TGACTACCTG
    ATGGCAGAGG AGAAGATCGT CATCTCCAAG AAAGAGTCCA TGTTCTCCTG GGATGAGACT
    GAGTACAAGA TCCCAGCTCG CCTCACCCTG CAGGTCTGGG ATGCCGACCA CTTCTCAGCC
    GATGATTTCC TTGGGGCCAT CGAGTTGGAC CTGAACCGCT TTCCGCGGGG AGCCAAGACA
    TCCAAACAGT GCAGTCTGGA GATGGTGACA AACGAGGCAG AGCTGCCCAT GGTCTCAATC
    TTCAAGCAAA AGCGGGTGAA GGGCTGGTGG CCATTTGTGG CCAGGGATGA GAATGACGAG
    CTGGAGATCA CCGGGAAAGT CGAGGCTGAA CTACACCTCC TGACTGCAGA GGAGGCAGAG
    AAATCTCCTG CTGGGCTGGC TCGCAATGAA CCCGACCCAC TGGAGAAACC CAACCGTCCA
    GACACGTCTT TCATCTGGTT CCTCAACCCT CTCAAGTCCA TCAAATACCT CATCTGCACC
    CGCTACAAGT GGCTCATCAT CAAAATCGTG CTGGCCCTGT TGCTCCTCAT CATGGTGGCC
    CTCTTCCTCT ACAGCATGCC AGGCTACATG GTCAAGAAGC TCCTGGGGGC ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34477
    Clone ID Related Accession (Same CDS sequence) XM_015285203.1 , XM_015285203.2
    Accession Version XM_015285203.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140689.1
    CDS153..6383
    Translation

    Target ORF information:

    RefSeq Version XM_015285203.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285203.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34477
    Clone ID Related Accession (Same CDS sequence) XM_015285203.1 , XM_015285203.2
    Accession Version XM_015285203.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285203.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140689.1
    CDS149..6379
    Translation

    Target ORF information:

    RefSeq Version XM_015285203.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285203.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGG
    ATGGAAGAGG ACGAGATGTA TTATCACAGC GAAGATGAAC TTCTGGGAGA GGGGGACACC
    CTTAGCCAGG GTTCCCTCAA CACGAGCCTT GGAGACGATG CGGAAGAAGA GCAGCACACT
    AGGATTAAGT TTAACGACGG ATACAAGTCA GTAGCAAGCG AAGACCCTGA AGACACCAGA
    GCTAAGGCTA AGGGGAAAGA GAAAAGACAC CTCATGTTGC CATTCAGCAA AGGCAAAGAG
    AAGACAAAGG AGCACAAAGC TGGGAAAGGA GTTTTTTCAG CCATGAAGCT TGGCAAGGCA
    CGTCCCACAA AAGATGACCA TAGGAAGCAA GATGAACCTG CTGTTCTGGA GGCAGAAGAC
    CTGGACCGAA AAGTCATGAG ACTGGGAGGG GGGCTGGACC CGGACACCAT CTCCTTGGCC
    TCTGTCACAG CTGTCACCAC CAATGTCTCA AACAAGAGGT CCAAACCTGA CATCAAAATG
    GAGCCAAGTG CTGGGAGGCC GATGGATTAC CAGGTCAGCA TCACTGTCAT CGAGGCCCGG
    CAGCTGGTTG GGCTCAACAT GGACCCCGTG GTCTGCGTGG AGGTTGGGGA GGAGAAGAAA
    TACACATCCA TGAAGGAATC GACCAACTGC CCTTACTACA ATGAGTACTT TGTCTTCGAT
    TTCCACGTGC CCCCGGATGT CATGTTTGAT AAGATCATCA AGCTGTCTGT GATCCACTCA
    AAAAACCTCT TGCGCAGTGG GACTTTGGTG GGCTCCTTCA AGATGGACGT TGGGACAGTT
    TACACCCAGC CTGAGCACCA GTTCTACCAC AAATGGGCCA TCCTTTCTGA CCCCGAGGAC
    CTCACTGCTG GGCTGAAAGG TTACCTGAAG TGTGACATCG CTGTTGTGGG GAAAGGGGAC
    AACATCAAGA CACCCCACAA AGCCAATGAG ACAGAGGAGG ATGATATTGA AGGGAACCTC
    CTCCTACCCG ATGGGGTTCC TCCAGAGAGG CAGTGGGCTC GTTTCTACAT CAAGATCTAT
    CGAGCAGAGG GCCTGCCCCG TATGAACACC AGCATCATGG CCAATGTGAA GAAGGCTCTC
    ATTGGAGAGA ATAAGGACTT GGTAGACCCC TACGTGCAAG TGGCATTTGC AGGGCAGAAG
    GGGAAGACAT CAGTGCAAAA AAGCAGCTAT GAGCCTCTCT GGAATGAGCA AATCATCTTC
    ACAGAAATGT TCCCTCCATT GTGCAAGCGG ATAAAGATCC AGATCCGAGA CTCGGACAAA
    GTCAATGATG TGGCCATTGG TACCCATTTC ATTGATTTGA GGAAGATCTC TAATGAAGGA
    GACAAAGGCT ACCTGCCCAC CTTTGGCCCT GCATGGGTGA ACATGTATGG CTCCACTCGG
    AACTACACCC TGATGGATGA ACACCAAGAG CTGAATGAGG GGCTGGGGGA AGGCGTCTCC
    TTCCGGGCCA GGCTGCTGAT GAGCCTTGCG GTTGAGATCT TGGATACCAC CAACCCAGAG
    ATCAACAGCT CCACTGAGGT GCAAGTGGAG CAGGCCACAC CAGTTGCTGA TAACTGCACT
    GGAAAGATGG AGGAGTTCTT TCTTTTTGGA GCCTTCCTGG AAGCTACCAT GATCGACAGG
    AAGATCGGTG ACAAACCCAT CAACTTTGAG GTTACAATAG GCAACTATGG GAACCAGATT
    GATGGCATGA ACAAACCTGT CCTGCGGAGG AAGAAGGAGG GAGGGGATGG GGACGAGGAG
    GAGTCCGAGC TGCTACACAA CTCAAGTGAG GATGAAGCTG ATGAGGATGG AGAGATGGTC
    TCTGTCTCCT CGTCTCAGCC CATGAAGCCC CTGGTTACAG ACAGGAACTA CTTCCACCTG
    CCGTACTTTG AGAAGAAGCC CTGCATCTAC ATCAAGAGCT GGTGGCAGGA TCAGCGCCGC
    AGGCTGTATA ACGCCAACAT CATGGACAAG ATTGCAGACA AGCTGGAGGA AGGGCTCAAT
    GATGTGCAGG AAATGATCAA GACAGAGAAA CCACATCCAG AGCGCCGGCT CCGAGGGGTC
    CTGGAGGAGC TGAGCAGCGG GTGCTTGCGC TTTGTGACGC TGGCTGACAA GGACCAGCAC
    CACTCTTCCC GCACGAGGTT GGACCGAGAG AGGCTCAAGT CCTGCATGCG GGAGCTGGAG
    AACATGGGCC AGCAAGCCAC GACTCTGAGG TCGCAGGTGA AGAAGAATAC CATGAAAGAC
    AAGCTGAAGC AGGTGCAGAA CTTCCTGCAG AAACTCCGTT TCTTGGCAGA TGAGCCCCAG
    CACACCATCC CTGACATCTT CATCTGGATG ATGAGCAATA ACAAGCGTAT TGCCTATGCC
    CGTGTCCCTT CCAAGGACAT CCTCTATTCC ATTGTGGATG AGGAGATGGG CAAGGACTGC
    GCCAAAGTGA AGACTGTCTT TCTCAAGCTG CCTGGGAAGA GGGGTTTCGG ACCTGCCGGC
    TGGACAGTTC AGGCCAAGAT GGAGATTTAC TTGTGGCTGG GCCTCAACAA GCAGCGCAAA
    GACTTCCTGA GTGGCTTGCC CTGTGGTTTT GAGGAGAAGA AGACCACCAG AGGCCAAAAC
    CTGCCGTCCT TCCCTCCCAT CAGCCTTCTT TACACCAAGA AACAAGTTTT CCAGCTGCGA
    GCCCACATGT ACCAGGCCAG GAGTTTATTT GCTGCTGATA GTAGTGGCCT TTCAGATCCC
    TTTGCCCGAG TTTTCTTCAC TTCCCAGAGC CAATGCACTG AGGTTCTCAA TGAGACCCTG
    TGCCCAACCT GGGACCAGCT GCTGGTTTTT GACAACGTGG AGCTGTACGG AGAAGCCCAT
    GAGATGCGGG ACGATCCTCC TATAATTGTC ATTGAGATCT ATGATCAAGA CACAGTGGGC
    AAAGCTGACT TCATGGGCCG CACCTTTGCC AAACCAGTGG TGAAAATGTC AGATGAGCAG
    TACTGCCCCC CACGCTTCCC CCCACAGCTG GAATACTACC AGATCTACCG GGGCAACTCT
    ACAGCAGGAG ACCTTCTGGC TGCCTTCGAG CTGCTCCAGA TTGGCTCTGG GGGCAAATCA
    GACCTGCCTC CAATTGATGG CCCCACAGAC ATAGACCGAG GCCCCATCCT GCCGGTGCCA
    CTGGGGATTC GGCCGGTGCT GAGTAAATAC AGAGTGGAGA TCTTGTTTTG GGGACTCAGG
    GACCTGAAGA GAGTGAACCT GGCCCAGGTG GACCGGCCCC GTGTGGACAT TGAGTGTGCT
    GGGAAGGGAG TTCAGTCAGC TCTCATCCAG AACTACAAGA AAAACCCAAA CTTCAGCACT
    TTGGTCAAGT GGTTTGAAGT GGATCTCCCA GAGAATGAGC TGCTCCACCC ACCTCTGAAC
    ATCCGAGTGG TGGACTGCCG GGCTTTTGGG CGCTACACCT TGGTGGGCTC CCATACCGTC
    AGCTCCCTCC GAAAGTTCAT CTACCGCCCA CCAGATAAGA AAGCCCAGCA CTGGAATATG
    ACAGGGGAGA TCATTGTCAA CATGGAACCT GAGGTTCCTA TCAAGAAGAT GGAGACCATG
    GTGAAGCTGG AAGCTAACTC CGACGCAGTG GTGAAGGTGG ATGTGTCTGA AGAAGAGAAG
    GAGAAAAAGA AAAAGAAGAA GAAAGGAGGT GGAGGAGGAG GAGGAGGAGA AGAGATAGAA
    GAAGAGGAGC CAGATGAGAG CATGCTGGAC TGGTGGTCCA AGTATTTTGC TTCAATTGAG
    ACTATGAAAG AGCAACTCCG GCAGCAGGAA GCAGCTGCAG CAGAAGCAGA GGAGAAGGAA
    GAGATGGAGA TTGCAGAGGG CTTCAAGGGC CAGGCAAAGA ACAAGGAGAA GTCCAAGGCA
    CCCAAAGATG ACAAGAAGAA GAAGCAGCAG TCAGCCCCTG AGCTCCCTGA GAAGAAGAAC
    AAGCAGAAGA TTGATGAACT GAAGGTCTTC AACAAAGAGC TGGAAGCAGA GTTTGATAAC
    TTTGAGGACT GGCTGCACAC CTTCAACCTG CTCCGTGGGA AGATTGGGGA CAATGATGAC
    AATGCAACAG AAGAAGAGCG GATAGTGGGA AGGTTCAAAG GCTCCATGTG TGTCTACAAA
    GTGCCTCTCC CTGATGATAT CACCAAGGAG GCAGGATATG ACCCCACCTT TGGCATGTTC
    CAGGGGATTC CCAGCAACGA CCCCATCAAT GTGCTGGTCC GAGTCTATAT TGTGAGGGCC
    ACAGACCTCC ACCCAGCTGA CATCAATGGG AAAGCTGACC CTTATATTGC CATCAAGCTG
    GGGAAGACAG ACATCAAAGA CAAGGAGAAC TATATCTCCA AGCAGCTGAA TCCTGTCTTC
    GGAAAGTCCT TTGACATTGA GGCCACCTTC CCCATGGAGT CCATGCTGAC AGTGGCAGTG
    TATGACTGGG ACTTGGTGGG GACAGATGAC CTCATTGGGG AGACCAAGAT TGACCTGGAG
    AACCGCTACT ACAGCAAGCA CCGAGCTACG TGTGGGGTGT CACAGACTTA CTCCATACAT
    GGGTATAACA TCTGGCGTGA CCCCATGAAG CCCTCCCAGA TCCTGTCCAA GCTGTGCAAA
    GAGGGCAAGG TGGATGGGCC ACACTTCGGG CCAGGAGGAA GAGTGAAAGT TGCCAACAGG
    GTGTACACTG GTCCCACGGA GATTGAGGAT GAAAATGGTC AGAAGAAACA GACAGATGAA
    CACTTGGCCC TGACCGTGTT GCGCCACTGG GAGGACGTCC CACGGGCAGG CTGCAAGCTG
    GTCCCTGAGC ACGTGGAGAC GCGCCCGCTG CTGAACCCTG ACAAGCCGGG CATTGAGCAG
    GGTCGCCTGG AGATGTGGGT GGATATGTTC CCCATGGATA TGCCGGCACC TGGTCCTGCC
    ATTGATATCT CCCCCAGGAA ACCAAAGAAA TATGAGCTCA GAGTGATAGT GTGGAACACA
    GATGAGGTCA TCCTGGAAGA TGATGACTAC TTCACTGGCG AGAAATCCAG TGACATTTTT
    GTCAGGGGGT GGCTGAAGGG TCAGCAGGAG GACAAGCAAG ACACAGACGT CCATTACCAC
    TCGCTCACCG GCGAAGGCAA CTTCAACTGG CGTTACATCT TCCCCTTTGA CTACCTGATG
    GCAGAGGAGA AGATCGTCAT CTCCAAGAAA GAGTCCATGT TCTCCTGGGA TGAGACTGAG
    TACAAGATCC CAGCTCGCCT CACCCTGCAG GTCTGGGATG CCGACCACTT CTCAGCCGAT
    GATTTCCTTG GGGCCATCGA GTTGGACCTG AACCGCTTTC CGCGGGGAGC CAAGACATCC
    AAACAGTGCA GTCTGGAGAT GGTGACAAAC GAGGCAGAGC TGCCCATGGT CTCAATCTTC
    AAGCAAAAGC GGGTGAAGGG CTGGTGGCCA TTTGTGGCCA GGGATGAGAA TGACGAGCTG
    GAGATCACCG GGAAAGTCGA GGCTGAACTA CACCTCCTGA CTGCAGAGGA GGCAGAGAAA
    TCTCCTGCTG GGCTGGCTCG CAATGAACCC GACCCACTGG AGAAACCCAA CCGTCCAGAC
    ACGTCTTTCA TCTGGTTCCT CAACCCTCTC AAGTCCATCA AATACCTCAT CTGCACCCGC
    TACAAGTGGC TCATCATCAA AATCGTGCTG GCCCTGTTGC TCCTCATCAT GGTGGCCCTC
    TTCCTCTACA GCATGCCAGG CTACATGGTC AAGAAGCTCC TGGGGGCATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34478
    Clone ID Related Accession (Same CDS sequence) XM_015285204.2 , XM_015285204.1
    Accession Version XM_015285204.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015285204.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140690.1
    CDS149..6211
    Translation

    Target ORF information:

    RefSeq Version XM_015285204.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285204.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa34478
    Clone ID Related Accession (Same CDS sequence) XM_015285204.2 , XM_015285204.1
    Accession Version XM_015285204.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015140690.1
    CDS153..6215
    Translation

    Target ORF information:

    RefSeq Version XM_015285204.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015285204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07075
    Clone ID Related Accession (Same CDS sequence) XM_420015.6 , XM_420015.5
    Accession Version XM_420015.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_420015.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420015.2
    CDS1..6033
    Translation

    Target ORF information:

    RefSeq Version XM_420015.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420015.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07075
    Clone ID Related Accession (Same CDS sequence) XM_420015.6 , XM_420015.5
    Accession Version XM_420015.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6033bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_420015.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_420015.2
    CDS153..6185
    Translation

    Target ORF information:

    RefSeq Version XM_420015.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_420015.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGCTAA ACAGCTCAAA
    GGCTACTTGG CAATGGCCAA CGGGGCCCCT CGCTCTCGCC CCACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GGCTTCAAGG GCCAGGCAAA GAACAAGGAG AAGTCCAAGG CACCCAAAGA TGACAAGAAG
    AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT GAGAAGAAGA ACAAGCAGAA GATTGATGAA
    CTGAAGGTCT TCAACAAAGA GCTGGAAGCA GAGTTTGATA ACTTTGAGGA CTGGCTGCAC
    ACCTTCAACC TGCTCCGTGG GAAGATTGGG GACAATGATG ACAATGCAAC AGAAGAAGAG
    CGGATAGTGG GAAGGTTCAA AGGCTCCATG TGTGTCTACA AAGTGCCTCT CCCTGATGAT
    ATCACCAAGG AGGCAGGATA TGACCCCACC TTTGGCATGT TCCAGGGGAT TCCCAGCAAC
    GACCCCATCA ATGTGCTGGT CCGAGTCTAT ATTGTGAGGG CCACAGACCT CCACCCAGCT
    GACATCAATG GGAAAGCTGA CCCTTATATT GCCATCAAGC TGGGGAAGAC AGACATCAAA
    GACAAGGAGA ACTATATCTC CAAGCAGCTG AATCCTGTCT TCGGAAAGTC CTTTGACATT
    GAGGCCACCT TCCCCATGGA GTCCATGCTG ACAGTGGCAG TGTATGACTG GGACTTGGTG
    GGGACAGATG ACCTCATTGG GGAGACCAAG ATTGACCTGG AGAACCGCTA CTACAGCAAG
    CACCGAGCTA CGTGTGGGGT GTCACAGACT TACTCCATAC ATGGGTATAA CATCTGGCGT
    GACCCCATGA AGCCCTCCCA GATCCTGTCC AAGCTGTGCA AAGAGGGCAA GGTGGATGGG
    CCACACTTCG GGCCAGGAGG AAGAGTGAAA GTTGCCAACA GGGTGTACAC TGGTCCCACG
    GAGATTGAGG ATGAAAATGG TCAGAAGAAA CAGACAGATG AACACTTGGC CCTGACCGTG
    TTGCGCCACT GGGAGGACGT CCCACGGGCA GGCTGCAAGC TGGTCCCTGA GCACGTGGAG
    ACGCGCCCGC TGCTGAACCC TGACAAGCCG GGCATTGAGC AGGGTCGCCT GGAGATGTGG
    GTGGATATGT TCCCCATGGA TATGCCGGCA CCTGGTCCTG CCATTGATAT CTCCCCCAGG
    AAACCAAAGA AATATGAGCT CAGAGTGATA GTGTGGAACA CAGATGAGGT CATCCTGGAA
    GATGATGACT ACTTCACTGG CGAGAAATCC AGTGACATTT TTGTCAGGGG GTGGCTGAAG
    GGTCAGCAGG AGGACAAGCA AGACACAGAC GTCCATTACC ACTCGCTCAC CGGCGAAGGC
    AACTTCAACT GGCGTTACAT CTTCCCCTTT GACTACCTGA TGGCAGAGGA GAAGATCGTC
    ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG GATGAGACTG AGTACAAGAT CCCAGCTCGC
    CTCACCCTGC AGGTCTGGGA TGCCGACCAC TTCTCAGCCG ATGATTTCCT TGGGGCCATC
    GAGTTGGACC TGAACCGCTT TCCGCGGGGA GCCAAGACAT CCAAACAGTG CAGTCTGGAG
    ATGGTGACAA ACGAGGCAGA GCTGCCCATG GTCTCAATCT TCAAGCAAAA GCGGGTGAAG
    GGCTGGTGGC CATTTGTGGC CAGGGATGAG AATGACGAGC TGGAGATCAC CGGGAAAGTC
    GAGGCTGAAC TACACCTCCT GACTGCAGAG GAGGCAGAGA AATCTCCTGC TGGGCTGGCT
    CGCAATGAAC CCGACCCACT GGAGAAACCC AACCGTCCAG ACACGTCTTT CATCTGGTTC
    CTCAACCCTC TCAAGTCCAT CAAATACCTC ATCTGCACCC GCTACAAGTG GCTCATCATC
    AAAATCGTGC TGGCCCTGTT GCTCCTCATC ATGGTGGCCC TCTTCCTCTA CAGCATGCCA
    GGCTACATGG TCAAGAAGCT CCTGGGGGCA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07076
    Clone ID Related Accession (Same CDS sequence) XM_004935857.2 , XM_004935857.3
    Accession Version XM_004935857.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455847.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004935857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935914.1
    CDS152..6154
    Translation

    Target ORF information:

    RefSeq Version XM_004935857.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa07076
    Clone ID Related Accession (Same CDS sequence) XM_004935857.2 , XM_004935857.3
    Accession Version XM_004935857.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6003bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product otoferlin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006090.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004935857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004935914.1
    CDS1..6003
    Translation

    Target ORF information:

    RefSeq Version XM_004935857.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus otoferlin (OTOF), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004935857.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGCTCTGC AGCTGCAGCT CCGCACTGTC TCCGGCCTCC GCGGCAGAGC GGATCGCATC 
    GCCAAAGCGG CTTTCCGAGG GCTCTCCTTC TACACCCGAG TGATGGAAAA CTGCGAGGAC
    GAGGCCAGGT TTGATGAGAC CTTTCGCTGG CCAGTTGCAA GCAACATCGA TGGGAACGAG
    ATCTTGGAGA TCCAGGTCTT CAACTACAGC AAAGTCTTCA CCAATAGGCT TATTGGCACT
    TTCCGGATGG TCCTGCAAAA GGTGGTGGAG GAAGGGCACG TGGAGGTGAC AGATACACTC
    ATAGATGACA ATAATTCGGC CATCCAGACC AGCATCTCCA TAGAAATCAG GTACCAGGCT
    CTGGATGGGA CAGTGGGTAC GTGGAATGAC AAGGAATTCC TGGAGACTCC CAGTGTCCAC
    TCAGAAGGGG ATGGCAGATA CCCTCTGGAG ACAGACAGCC TCCTCTCCGG CCACCGGCAG
    AGCACAGATG GGTCTGTGGG CAAATCCAAC CAGCAGTCCA CAGAGAGAAG CTTCAGGAGA
    GCTGGGAAAG GAGTTTTTTC AGCCATGAAG CTTGGCAAGG CACGTCCCAC AAAAGATGAC
    CATAGGAAGC AAGATGAACC TGCTGTTCTG GAGGCAGAAG ACCTGGACCG AAAAGTCATG
    AGACTGGGAG GGGGGCTGGA CCCGGACACC ATCTCCTTGG CCTCTGTCAC AGCTGTCACC
    ACCAATGTCT CAAACAAGAG GTCCAAACCT GACATCAAAA TGGAGCCAAG TGCTGGGAGG
    CCGATGGATT ACCAGGTCAG CATCACTGTC ATCGAGGCCC GGCAGCTGGT TGGGCTCAAC
    ATGGACCCCG TGGTCTGCGT GGAGGTTGGG GAGGAGAAGA AATACACATC CATGAAGGAA
    TCGACCAACT GCCCTTACTA CAATGAGTAC TTTGTCTTCG ATTTCCACGT GCCCCCGGAT
    GTCATGTTTG ATAAGATCAT CAAGCTGTCT GTGATCCACT CAAAAAACCT CTTGCGCAGT
    GGGACTTTGG TGGGCTCCTT CAAGATGGAC GTTGGGACAG TTTACACCCA GCCTGAGCAC
    CAGTTCTACC ACAAATGGGC CATCCTTTCT GACCCCGAGG ACCTCACTGC TGGGCTGAAA
    GGTTACCTGA AGTGTGACAT CGCTGTTGTG GGGAAAGGGG ACAACATCAA GACACCCCAC
    AAAGCCAATG AGACAGAGGA GGATGATATT GAAGGGAACC TCCTCCTACC CGATGGGGTT
    CCTCCAGAGA GGCAGTGGGC TCGTTTCTAC ATCAAGATCT ATCGAGCAGA GGGCCTGCCC
    CGTATGAACA CCAGCATCAT GGCCAATGTG AAGAAGGCTC TCATTGGAGA GAATAAGGAC
    TTGGTAGACC CCTACGTGCA AGTGGCATTT GCAGGGCAGA AGGGGAAGAC ATCAGTGCAA
    AAAAGCAGCT ATGAGCCTCT CTGGAATGAG CAAATCATCT TCACAGAAAT GTTCCCTCCA
    TTGTGCAAGC GGATAAAGAT CCAGATCCGA GACTCGGACA AAGTCAATGA TGTGGCCATT
    GGTACCCATT TCATTGATTT GAGGAAGATC TCTAATGAAG GAGACAAAGG CTACCTGCCC
    ACCTTTGGCC CTGCATGGGT GAACATGTAT GGCTCCACTC GGAACTACAC CCTGATGGAT
    GAACACCAAG AGCTGAATGA GGGGCTGGGG GAAGGCGTCT CCTTCCGGGC CAGGCTGCTG
    ATGAGCCTTG CGGTTGAGAT CTTGGATACC ACCAACCCAG AGATCAACAG CTCCACTGAG
    GTGCAAGTGG AGCAGGCCAC ACCAGTTGCT GATAACTGCA CTGGAAAGAT GGAGGAGTTC
    TTTCTTTTTG GAGCCTTCCT GGAAGCTACC ATGATCGACA GGAAGATCGG TGACAAACCC
    ATCAACTTTG AGGTTACAAT AGGCAACTAT GGGAACCAGA TTGATGGCAT GAACAAACCT
    GTCCTGCGGA GGAAGAAGGA GGGAGGGGAT GGGGACGAGG AGGAGTCCGA GCTGCTACAC
    AACTCAAGTG AGGATGAAGC TGATGAGGAT GGAGAGATGG TCTCTGTCTC CTCGTCTCAG
    CCCATGAAGC CCCTGGTTAC AGACAGGAAC TACTTCCACC TGCCGTACTT TGAGAAGAAG
    CCCTGCATCT ACATCAAGAG CTGGTGGCAG GATCAGCGCC GCAGGCTGTA TAACGCCAAC
    ATCATGGACA AGATTGCAGA CAAGCTGGAG GAAGGGCTCA ATGATGTGCA GGAAATGATC
    AAGACAGAGA AACCACATCC AGAGCGCCGG CTCCGAGGGG TCCTGGAGGA GCTGAGCAGC
    GGGTGCTTGC GCTTTGTGAC GCTGGCTGAC AAGGACCAGC ACCACTCTTC CCGCACGAGG
    TTGGACCGAG AGAGGCTCAA GTCCTGCATG CGGGAGCTGG AGAACATGGG CCAGCAAGCC
    ACGACTCTGA GGTCGCAGGT GAAGAAGAAT ACCATGAAAG ACAAGCTGAA GCAGGTGCAG
    AACTTCCTGC AGAAACTCCG TTTCTTGGCA GATGAGCCCC AGCACACCAT CCCTGACATC
    TTCATCTGGA TGATGAGCAA TAACAAGCGT ATTGCCTATG CCCGTGTCCC TTCCAAGGAC
    ATCCTCTATT CCATTGTGGA TGAGGAGATG GGCAAGGACT GCGCCAAAGT GAAGACTGTC
    TTTCTCAAGC TGCCTGGGAA GAGGGGTTTC GGACCTGCCG GCTGGACAGT TCAGGCCAAG
    ATGGAGATTT ACTTGTGGCT GGGCCTCAAC AAGCAGCGCA AAGACTTCCT GAGTGGCTTG
    CCCTGTGGTT TTGAGGAGAA GAAGACCACC AGAGGCCAAA ACCTGCCGTC CTTCCCTCCC
    ATCAGCCTTC TTTACACCAA GAAACAAGTT TTCCAGCTGC GAGCCCACAT GTACCAGGCC
    AGGAGTTTAT TTGCTGCTGA TAGTAGTGGC CTTTCAGATC CCTTTGCCCG AGTTTTCTTC
    ACTTCCCAGA GCCAATGCAC TGAGGTTCTC AATGAGACCC TGTGCCCAAC CTGGGACCAG
    CTGCTGGTTT TTGACAACGT GGAGCTGTAC GGAGAAGCCC ATGAGATGCG GGACGATCCT
    CCTATAATTG TCATTGAGAT CTATGATCAA GACACAGTGG GCAAAGCTGA CTTCATGGGC
    CGCACCTTTG CCAAACCAGT GGTGAAAATG TCAGATGAGC AGTACTGCCC CCCACGCTTC
    CCCCCACAGC TGGAATACTA CCAGATCTAC CGGGGCAACT CTACAGCAGG AGACCTTCTG
    GCTGCCTTCG AGCTGCTCCA GATTGGCTCT GGGGGCAAAT CAGACCTGCC TCCAATTGAT
    GGCCCCACAG ACATAGACCG AGGCCCCATC CTGCCGGTGC CACTGGGGAT TCGGCCGGTG
    CTGAGTAAAT ACAGAGTGGA GATCTTGTTT TGGGGACTCA GGGACCTGAA GAGAGTGAAC
    CTGGCCCAGG TGGACCGGCC CCGTGTGGAC ATTGAGTGTG CTGGGAAGGG AGTTCAGTCA
    GCTCTCATCC AGAACTACAA GAAAAACCCA AACTTCAGCA CTTTGGTCAA GTGGTTTGAA
    GTGGATCTCC CAGAGAATGA GCTGCTCCAC CCACCTCTGA ACATCCGAGT GGTGGACTGC
    CGGGCTTTTG GGCGCTACAC CTTGGTGGGC TCCCATACCG TCAGCTCCCT CCGAAAGTTC
    ATCTACCGCC CACCAGATAA GAAAGCCCAG CACTGGAATA TGACAGGGGA GATCATTGTC
    AACATGGAAC CTGAGGTTCC TATCAAGAAG ATGGAGACCA TGGTGAAGCT GGAAGCTAAC
    TCCGACGCAG TGGTGAAGGT GGATGTGTCT GAAGAAGAGA AGGAGAAAAA GAAAAAGAAG
    AAGAAAGGAG GTGGAGGAGG AGGAGGAGGA GAAGAGATAG AAGAAGAGGA GCCAGATGAG
    AGCATGCTGG ACTGGTGGTC CAAGTATTTT GCTTCAATTG AGACTATGAA AGAGCAACTC
    CGGCAGCAGG AAGCAGCTGC AGCAGAAGCA GAGGAGAAGG AAGAGATGGA GATTGCAGAG
    GAAATCAAAC TTGATGACTC TCCCATGAAA GGCTTCAAGG GCCAGGCAAA GAACAAGGAG
    AAGTCCAAGG CACCCAAAGA TGACAAGAAG AAGAAGCAGC AGTCAGCCCC TGAGCTCCCT
    GAGAAGAAGA ACAAGCAGAA GATTGATGAA CTGAAGGTCT TCAACAAAGA GCTGGAAGCA
    GAGTTTGATA ACTTTGAGGA CTGGCTGCAC ACCTTCAACC TGCTCCGTGG GAAGATTGGG
    GACAATGATG ACAATGCAAC AGAAGAAGAG CGGATAGTGG GAAGGTTCAA AGGCTCCATG
    TGTGTCTACA AAGTGCCTCT CCCTGATGAT ATCACCAAGG AGGCAGGATA TGACCCCACC
    TTTGGCATGT TCCAGGGGAT TCCCAGCAAC GACCCCATCA ATGTGCTGGT CCGAGTCTAT
    ATTGTGAGGG CCACAGACCT CCACCCAGCT GACATCAATG GGAAAGCTGA CCCTTATATT
    GCCATCAAGC TGGGGAAGAC AGACATCAAA GACAAGGAGA ACTATATCTC CAAGCAGCTG
    AATCCTGTCT TCGGAAAGTC CTTTGACATT GAGGCCACCT TCCCCATGGA GTCCATGCTG
    ACAGTGGCAG TGTATGACTG GGACTTGGTG GGGACAGATG ACCTCATTGG GGAGACCAAG
    ATTGACCTGG AGAACCGCTA CTACAGCAAG CACCGAGCTA CGTGTGGGGT GTCACAGACT
    TACTCCATAC ATGGGTATAA CATCTGGCGT GACCCCATGA AGCCCTCCCA GATCCTGTCC
    AAGCTGTGCA AAGAGGGCAA GGTGGATGGG CCACACTTCG GGCCAGGAGG AAGAGTGAAA
    GTTGCCAACA GGGTGTACAC TGGTCCCACG GAGATTGAGG ATGAAAATGG TCAGAAGAAA
    CAGACAGATG AACACTTGGC CCTGACCGTG TTGCGCCACT GGGAGGACGT CCCACGGGCA
    GGCTGCAAGC TGGTCCCTGA GCACGTGGAG ACGCGCCCGC TGCTGAACCC TGACAAGCCG
    GGCATTGAGC AGGGTCGCCT GGAGATGTGG GTGGATATGT TCCCCATGGA TATGCCGGCA
    CCTGGTCCTG CCATTGATAT CTCCCCCAGG AAACCAAAGA AATATGAGCT CAGAGTGATA
    GTGTGGAACA CAGATGAGGT CATCCTGGAA GATGATGACT ACTTCACTGG CGAGAAATCC
    AGTGACATTT TTGTCAGGGG GTGGCTGAAG GGTCAGCAGG AGGACAAGCA AGACACAGAC
    GTCCATTACC ACTCGCTCAC CGGCGAAGGC AACTTCAACT GGCGTTACAT CTTCCCCTTT
    GACTACCTGA TGGCAGAGGA GAAGATCGTC ATCTCCAAGA AAGAGTCCAT GTTCTCCTGG
    GATGAGACTG AGTACAAGAT CCCAGCTCGC CTCACCCTGC AGGTCTGGGA TGCCGACCAC
    TTCTCAGCCG ATGATTTCCT TGGGGCCATC GAGTTGGACC TGAACCGCTT TCCGCGGGGA
    GCCAAGACAT CCAAACAGTG CAGTCTGGAG ATGGTGACAA ACGAGGCAGA GCTGCCCATG
    GTCTCAATCT TCAAGCAAAA GCGGGTGAAG GGCTGGTGGC CATTTGTGGC CAGGGATGAG
    AATGACGAGC TGGAGATCAC CGGGAAAGTC GAGGCTGAAC TACACCTCCT GACTGCAGAG
    GAGGCAGAGA AATCTCCTGC TGGGCTGGCT CGCAATGAAC CCGACCCACT GGAGAAACCC
    AACCGTCCAG ACACGTCTTT CATCTGGTTC CTCAACCCTC TCAAGTCCAT CAAATACCTC
    ATCTGCACCC GCTACAAGTG GCTCATCATC AAAATCGTGC TGGCCCTGTT GCTCCTCATC
    ATGGTGGCCC TCTTCCTCTA CAGCATGCCA GGCTACATGG TCAAGAAGCT CCTGGGGGCA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.