OTOF gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017005338.1	XP_016860827.1	otoferlin isoform X1
NM_194322.2	NP_919303.1	otoferlin isoform c
NM_194248.2	NP_919224.1	otoferlin isoform a
NM_194248.3	NP_919224.1	otoferlin isoform a
NM_001287489.2	NP_001274418.1	otoferlin isoform e
NM_004802.4	NP_004793.2	otoferlin isoform b
NM_001287489.1	NP_001274418.1	otoferlin isoform e
NM_194323.3	NP_919304.1	otoferlin isoform d
NM_004802.3	NP_004793.2	otoferlin isoform b
NM_194323.2	NP_919304.1	otoferlin isoform d
NM_194322.3	NP_919303.1	otoferlin isoform c

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28619	NM_194322.3 Latest version!	Homo sapiens otoferlin (OTOF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu28598	NM_004802.4 Latest version!	Homo sapiens otoferlin (OTOF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$874.30 $1249.00
OHu28603	NM_194248.3 Latest version!	Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu28605	NM_001287489.2 Latest version!	Homo sapiens otoferlin (OTOF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu28620	NM_194323.3 Latest version!	Homo sapiens otoferlin (OTOF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$874.30 $1249.00
OHu92767	XM_017005338.1 Latest version!	Homo sapiens otoferlin (OTOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu28603	NM_194248.2	Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu28605	NM_001287489.1	Homo sapiens otoferlin (OTOF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu28598	NM_004802.3	Homo sapiens otoferlin (OTOF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$874.30 $1249.00
OHu28620	NM_194323.2	Homo sapiens otoferlin (OTOF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$874.30 $1249.00
OHu28619	NM_194322.2	Homo sapiens otoferlin (OTOF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

OTOF ( NM_194322.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_919303.1 Homo sapiens otoferlin (OTOF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28619
Clone ID Related Accession (Same CDS sequence)	NM_194322.2 , NM_194322.3
Accession Version	NM_194322.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	otoferlin isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093378.5, AF183186.1 and AF183187.1. On Dec 2, 2008 this sequence version replaced NM_194322.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c, also called 'short form 2') has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGACCG ATACTCAGGA TGGCCCTAGC GAGAGCTCCC AGATCATGAG GTCCCTCACT 
CCCCTGATCA ACAGGGAGGA GGCATTTGGG GAGGCTGGGG AGGCGGGGCT GTGGCCCAGC 
ATCACCCACA CTCCTGATTC ACAGGAAGAA GGCCTGAACG ACATACAGGA GATGATCAAA 
ACGGAGAAGT CCTACCCTGA GCGTCGCCTG CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC 
TGCTGCCGCT TCCTCTCCCT CGCTGACAAG GACCAGGGCC ACTCATCCCG CACCAGGCTT 
GACCGGGAGC GCCTCAAGTC CTGCATGAGG GAGCTGGAAA ACATGGGGCA GCAGGCCAGG 
ATGCTGCGGG CCCAGGTGAA GCGGCACACG GTGCGGGACA AGCTGAGGCT GTGCCAGAAC 
TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC GAGCCCCAGC ACAGCATTCC CGACATCTTC 
ATCTGGATGA TGAGCAACAA CAAGCGTGTC GCCTATGCCC GTGTGCCCTC CAAGGACCTG 
CTCTTCTCCA TCGTGGAGGA GGAGACTGGC AAGGACTGCG CCAAGGTCAA GACGCTCTTC 
CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC TCGGCAGGCT GGACAGTGCA GGCCAAGGTG 
GAGCTGTACC TGTGGCTGGG CCTCAGCAAA CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC 
TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG GGCCTGGGCC TGCATGCCTT CCCACCCGTC 
AGCCTGGTCT ACACCAAGAA GCAGGCGTTC CAGCTCCGAG CGCACATGTA CCAGGCCCGC 
AGCCTCTTTG CCGCCGACAG CAGCGGACTC TCAGACCCCT TTGCCCGCGT CTTCTTCATC 
AATCAGAGTC AGTGCACAGA GGTGCTGAAT GAGACCCTGT GTCCCACCTG GGACCAGATG 
CTGGTGTTCG ACAACCTGGA GCTCTATGGT GAAGCTCATG AGCTGAGGGA CGATCCGCCC 
ATCATTGTCA TTGAAATCTA TGACCAGGAT TCCATGGGCA AAGCTGACTT CATGGGCCGG 
ACCTTCGCCA AACCCCTGGT GAAGATGGCA GACGAGGCGT ACTGCCCACC CCGCTTCCCA 
CCTCAGCTCG AGTACTACCA GATCTACCGT GGCAACGCCA CAGCTGGAGA CCTGCTGGCG 
GCCTTCGAGC TGCTGCAGAT TGGACCAGCA GGGAAGGCTG ACCTGCCCCC CATCAATGGC 
CCGGTGGACG TGGACCGAGG TCCCATCATG CCCGTGCCCA TGGGCATCCG GCCCGTGCTC 
AGCAAGTACC GAGTGGAGGT GCTGTTCTGG GGCCTACGGG ACCTAAAGCG GGTGAACCTG 
GCCCAGGTGG ACCGGCCACG GGTGGACATC GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC 
CTGATCCACA ATTATAAGAA GAACCCCAAC TTCAACACCC TCGTCAAGTG GTTTGAAGTG 
GACCTCCCAG AGAACGAGCT GCTGCACCCG CCCTTGAACA TCCGTGTGGT GGACTGCCGG 
GCCTTCGGTC GCTACACACT GGTGGGCTCC CATGCCGTCA GCTCCCTGCG ACGCTTCATC 
TACCGGCCCC CAGACCGCTC GGCCCCCAGC TGGAACACCA CGGTCAGGCT TCTCCGGCGC 
TGCCGTGTGC TGTGCAATGG GGGCTCCTCC TCTCACTCCA CAGGGGAGGT TGTGGTGACT 
ATGGAGCCAG AGGTACCCAT CAAGAAACTG GAGACCATGG TGAAGCTGGA CGCGACTTCT 
GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG 
GGCACTGCGG AGGAGCCAGA GGAGGAGGAG CCAGACGAGA GCATGCTGGA CTGGTGGTCC 
AAGTACTTTG CCTCCATTGA CACCATGAAG GAGCAACTTC GACAACAAGA GCCCTCTGGA 
ATTGACTTGG AGGAGAAGGA GGAAGTGGAC AATACCGAGG GCCTGAAGGG GTCAATGAAG 
GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG GAGAAGAAGA AGAAAACTCA GAGCTCTGGC 
TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG AAGAAACCCA AGATTGATGA GCTTAAGGTA 
TACCCCAAAG AGCTGGAGTC CGAGTTTGAT AACTTTGAGG ACTGGCTGCA CACTTTCAAC 
TTGCTTCGGG GCAAGACCGG GGATGATGAG GATGGCTCCA CCGAGGAGGA GCGCATTGTG 
GGACGCTTCA AGGGCTCCCT CTGCGTGTAC AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG 
GAAGCCGGCT ACGACTCCAC CTACGGCATG TTCCAGGGCA TCCCGAGCAA TGACCCCATC 
AATGTGCTGG TCCGAGTCTA TGTGGTCCGG GCCACGGACC TGCACCCTGC TGACATCAAC 
GGCAAAGCTG ACCCCTACAT CGCCATCCGG CTAGGCAAGA CTGACATCCG CGACAAGGAG 
AACTACATCT CCAAGCAGCT CAACCCTGTC TTTGGGAAGT CCTTTGACAT CGAGGCCTCC 
TTCCCCATGG AATCCATGCT GACGGTGGCT GTGTATGACT GGGACCTGGT GGGCACTGAT 
GACCTCATTG GGGAAACCAA GATCGACCTG GAGAACCGCT TCTACAGCAA GCACCGCGCC 
ACCTGCGGCA TCGCCCAGAC CTACTCCACA CATGGCTACA ATATCTGGCG GGACCCCATG 
AAGCCCAGCC AGATCCTGAC CCGCCTCTGC AAAGACGGCA AAGTGGACGG CCCCCACTTT 
GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC CGCGTCTTCA CTGGGCCCTC TGAGATTGAG 
GACGAGAACG GTCAGAGGAA GCCCACAGAC GAGCATGTGG CGCTGTTGGC CCTGAGGCAC 
TGGGAGGACA TCCCCCGCGC AGGCTGCCGC CTGGTGCCAG AGCATGTGGA GACGAGGCCG 
CTGCTCAACC CCGACAAGCC GGGCATCGAG CAGGGCCGCC TGGAGCTGTG GGTGGACATG 
TTCCCCATGG ACATGCCAGC CCCTGGGACG CCTCTGGACA TCTCACCTCG GAAGCCCAAG 
AAGTACGAGC TGCGGGTCAT CATCTGGAAC ACAGATGAGG TGGTCTTGGA GGACGACGAC 
TTCTTCACAG GGGAGAAGTC CAGTGACATC TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG 
GAGGACAAGC AGGACACAGA CGTCCACTAC CACTCCCTCA CTGGCGAGGG CAACTTCAAC 
TGGCGCTACC TGTTCCCCTT CGACTACCTG GCGGCGGAGG AGAAGATCGT CATCTCCAAG 
AAGGAGTCCA TGTTCTCCTG GGACGAGACC GAGTACAAGA TCCCCGCGCG GCTCACCCTG 
CAGATCTGGG ATGCGGACCA CTTCTCCGCT GACGACTTCC TGGGGGCCAT CGAGCTGGAC 
CTGAACCGGT TCCCGCGGGG CGCAAAGACA GCCAAGCAGT GCACCATGGA GATGGCCACC 
GGGGAGGTGG ACGTGCCCCT CGTGTCCATC TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG 
CCCCTCCTGG CCCGCAATGA GAACGATGAG TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG 
CTGCATTTAC TGACAGCAGA GGAGGCAGAG AAGAACCCAG TGGGCCTGGC CCGCAATGAA 
CCTGACCCCC TAGAGAAACC CAACCGGCCC GACACGAGCT TCATCTGGTT CCTGAACCCT 
CTCAAGTCGG CTCGCTACTT CTTGTGGCAC ACGTATCGCT GGCTGCTCCT CAAACTGTTG 
CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC CTGTTCCTCT ACTCTGTGCC TGGCTACCTG 
GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919303.1
CDS	150..4073
Translation	MMTDTQDGPSESSQIMRSLTPLINREEAFGEAGEAGLWPSITHTPDSQEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	NM_194322.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194322.2

ORF Insert Sequence:

ATGATGACCG ATACTCAGGA TGGCCCTAGC GAGAGCTCCC AGATCATGAG GTCCCTCACT 
CCCCTGATCA ACAGGGAGGA GGCATTTGGG GAGGCTGGGG AGGCGGGGCT GTGGCCCAGC 
ATCACCCACA CTCCTGATTC ACAGGAAGAA GGCCTGAACG ACATACAGGA GATGATCAAA 
ACGGAGAAGT CCTACCCTGA GCGTCGCCTG CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC 
TGCTGCCGCT TCCTCTCCCT CGCTGACAAG GACCAGGGCC ACTCATCCCG CACCAGGCTT 
GACCGGGAGC GCCTCAAGTC CTGCATGAGG GAGCTGGAAA ACATGGGGCA GCAGGCCAGG 
ATGCTGCGGG CCCAGGTGAA GCGGCACACG GTGCGGGACA AGCTGAGGCT GTGCCAGAAC 
TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC GAGCCCCAGC ACAGCATTCC CGACATCTTC 
ATCTGGATGA TGAGCAACAA CAAGCGTGTC GCCTATGCCC GTGTGCCCTC CAAGGACCTG 
CTCTTCTCCA TCGTGGAGGA GGAGACTGGC AAGGACTGCG CCAAGGTCAA GACGCTCTTC 
CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC TCGGCAGGCT GGACAGTGCA GGCCAAGGTG 
GAGCTGTACC TGTGGCTGGG CCTCAGCAAA CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC 
TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG GGCCTGGGCC TGCATGCCTT CCCACCCGTC 
AGCCTGGTCT ACACCAAGAA GCAGGCGTTC CAGCTCCGAG CGCACATGTA CCAGGCCCGC 
AGCCTCTTTG CCGCCGACAG CAGCGGACTC TCAGACCCCT TTGCCCGCGT CTTCTTCATC 
AATCAGAGTC AGTGCACAGA GGTGCTGAAT GAGACCCTGT GTCCCACCTG GGACCAGATG 
CTGGTGTTCG ACAACCTGGA GCTCTATGGT GAAGCTCATG AGCTGAGGGA CGATCCGCCC 
ATCATTGTCA TTGAAATCTA TGACCAGGAT TCCATGGGCA AAGCTGACTT CATGGGCCGG 
ACCTTCGCCA AACCCCTGGT GAAGATGGCA GACGAGGCGT ACTGCCCACC CCGCTTCCCA 
CCTCAGCTCG AGTACTACCA GATCTACCGT GGCAACGCCA CAGCTGGAGA CCTGCTGGCG 
GCCTTCGAGC TGCTGCAGAT TGGACCAGCA GGGAAGGCTG ACCTGCCCCC CATCAATGGC 
CCGGTGGACG TGGACCGAGG TCCCATCATG CCCGTGCCCA TGGGCATCCG GCCCGTGCTC 
AGCAAGTACC GAGTGGAGGT GCTGTTCTGG GGCCTACGGG ACCTAAAGCG GGTGAACCTG 
GCCCAGGTGG ACCGGCCACG GGTGGACATC GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC 
CTGATCCACA ATTATAAGAA GAACCCCAAC TTCAACACCC TCGTCAAGTG GTTTGAAGTG 
GACCTCCCAG AGAACGAGCT GCTGCACCCG CCCTTGAACA TCCGTGTGGT GGACTGCCGG 
GCCTTCGGTC GCTACACACT GGTGGGCTCC CATGCCGTCA GCTCCCTGCG ACGCTTCATC 
TACCGGCCCC CAGACCGCTC GGCCCCCAGC TGGAACACCA CGGTCAGGCT TCTCCGGCGC 
TGCCGTGTGC TGTGCAATGG GGGCTCCTCC TCTCACTCCA CAGGGGAGGT TGTGGTGACT 
ATGGAGCCAG AGGTACCCAT CAAGAAACTG GAGACCATGG TGAAGCTGGA CGCGACTTCT 
GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG 
GGCACTGCGG AGGAGCCAGA GGAGGAGGAG CCAGACGAGA GCATGCTGGA CTGGTGGTCC 
AAGTACTTTG CCTCCATTGA CACCATGAAG GAGCAACTTC GACAACAAGA GCCCTCTGGA 
ATTGACTTGG AGGAGAAGGA GGAAGTGGAC AATACCGAGG GCCTGAAGGG GTCAATGAAG 
GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG GAGAAGAAGA AGAAAACTCA GAGCTCTGGC 
TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG AAGAAACCCA AGATTGATGA GCTTAAGGTA 
TACCCCAAAG AGCTGGAGTC CGAGTTTGAT AACTTTGAGG ACTGGCTGCA CACTTTCAAC 
TTGCTTCGGG GCAAGACCGG GGATGATGAG GATGGCTCCA CCGAGGAGGA GCGCATTGTG 
GGACGCTTCA AGGGCTCCCT CTGCGTGTAC AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG 
GAAGCCGGCT ACGACTCCAC CTACGGCATG TTCCAGGGCA TCCCGAGCAA TGACCCCATC 
AATGTGCTGG TCCGAGTCTA TGTGGTCCGG GCCACGGACC TGCACCCTGC TGACATCAAC 
GGCAAAGCTG ACCCCTACAT CGCCATCCGG CTAGGCAAGA CTGACATCCG CGACAAGGAG 
AACTACATCT CCAAGCAGCT CAACCCTGTC TTTGGGAAGT CCTTTGACAT CGAGGCCTCC 
TTCCCCATGG AATCCATGCT GACGGTGGCT GTGTATGACT GGGACCTGGT GGGCACTGAT 
GACCTCATTG GGGAAACCAA GATCGACCTG GAGAACCGCT TCTACAGCAA GCACCGCGCC 
ACCTGCGGCA TCGCCCAGAC CTACTCCACA CATGGCTACA ATATCTGGCG GGACCCCATG 
AAGCCCAGCC AGATCCTGAC CCGCCTCTGC AAAGACGGCA AAGTGGACGG CCCCCACTTT 
GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC CGCGTCTTCA CTGGGCCCTC TGAGATTGAG 
GACGAGAACG GTCAGAGGAA GCCCACAGAC GAGCATGTGG CGCTGTTGGC CCTGAGGCAC 
TGGGAGGACA TCCCCCGCGC AGGCTGCCGC CTGGTGCCAG AGCATGTGGA GACGAGGCCG 
CTGCTCAACC CCGACAAGCC GGGCATCGAG CAGGGCCGCC TGGAGCTGTG GGTGGACATG 
TTCCCCATGG ACATGCCAGC CCCTGGGACG CCTCTGGACA TCTCACCTCG GAAGCCCAAG 
AAGTACGAGC TGCGGGTCAT CATCTGGAAC ACAGATGAGG TGGTCTTGGA GGACGACGAC 
TTCTTCACAG GGGAGAAGTC CAGTGACATC TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG 
GAGGACAAGC AGGACACAGA CGTCCACTAC CACTCCCTCA CTGGCGAGGG CAACTTCAAC 
TGGCGCTACC TGTTCCCCTT CGACTACCTG GCGGCGGAGG AGAAGATCGT CATCTCCAAG 
AAGGAGTCCA TGTTCTCCTG GGACGAGACC GAGTACAAGA TCCCCGCGCG GCTCACCCTG 
CAGATCTGGG ATGCGGACCA CTTCTCCGCT GACGACTTCC TGGGGGCCAT CGAGCTGGAC 
CTGAACCGGT TCCCGCGGGG CGCAAAGACA GCCAAGCAGT GCACCATGGA GATGGCCACC 
GGGGAGGTGG ACGTGCCCCT CGTGTCCATC TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG 
CCCCTCCTGG CCCGCAATGA GAACGATGAG TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG 
CTGCATTTAC TGACAGCAGA GGAGGCAGAG AAGAACCCAG TGGGCCTGGC CCGCAATGAA 
CCTGACCCCC TAGAGAAACC CAACCGGCCC GACACGAGCT TCATCTGGTT CCTGAACCCT 
CTCAAGTCGG CTCGCTACTT CTTGTGGCAC ACGTATCGCT GGCTGCTCCT CAAACTGTTG 
CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC CTGTTCCTCT ACTCTGTGCC TGGCTACCTG 
GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_194322.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_919303.1 Homo sapiens otoferlin (OTOF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28619
Clone ID Related Accession (Same CDS sequence)	NM_194322.2 , NM_194322.3
Accession Version	NM_194322.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	otoferlin isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093378.5, AF183186.1 and AF183187.1. On Jun 2, 2019 this sequence version replaced NM_194322.2. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c, also called 'short form 2') has a shorter and distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGACCG ATACTCAGGA TGGCCCTAGC GAGAGCTCCC AGATCATGAG GTCCCTCACT 
CCCCTGATCA ACAGGGAGGA GGCATTTGGG GAGGCTGGGG AGGCGGGGCT GTGGCCCAGC 
ATCACCCACA CTCCTGATTC ACAGGAAGAA GGCCTGAACG ACATACAGGA GATGATCAAA 
ACGGAGAAGT CCTACCCTGA GCGTCGCCTG CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC 
TGCTGCCGCT TCCTCTCCCT CGCTGACAAG GACCAGGGCC ACTCATCCCG CACCAGGCTT 
GACCGGGAGC GCCTCAAGTC CTGCATGAGG GAGCTGGAAA ACATGGGGCA GCAGGCCAGG 
ATGCTGCGGG CCCAGGTGAA GCGGCACACG GTGCGGGACA AGCTGAGGCT GTGCCAGAAC 
TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC GAGCCCCAGC ACAGCATTCC CGACATCTTC 
ATCTGGATGA TGAGCAACAA CAAGCGTGTC GCCTATGCCC GTGTGCCCTC CAAGGACCTG 
CTCTTCTCCA TCGTGGAGGA GGAGACTGGC AAGGACTGCG CCAAGGTCAA GACGCTCTTC 
CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC TCGGCAGGCT GGACAGTGCA GGCCAAGGTG 
GAGCTGTACC TGTGGCTGGG CCTCAGCAAA CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC 
TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG GGCCTGGGCC TGCATGCCTT CCCACCCGTC 
AGCCTGGTCT ACACCAAGAA GCAGGCGTTC CAGCTCCGAG CGCACATGTA CCAGGCCCGC 
AGCCTCTTTG CCGCCGACAG CAGCGGACTC TCAGACCCCT TTGCCCGCGT CTTCTTCATC 
AATCAGAGTC AGTGCACAGA GGTGCTGAAT GAGACCCTGT GTCCCACCTG GGACCAGATG 
CTGGTGTTCG ACAACCTGGA GCTCTATGGT GAAGCTCATG AGCTGAGGGA CGATCCGCCC 
ATCATTGTCA TTGAAATCTA TGACCAGGAT TCCATGGGCA AAGCTGACTT CATGGGCCGG 
ACCTTCGCCA AACCCCTGGT GAAGATGGCA GACGAGGCGT ACTGCCCACC CCGCTTCCCA 
CCTCAGCTCG AGTACTACCA GATCTACCGT GGCAACGCCA CAGCTGGAGA CCTGCTGGCG 
GCCTTCGAGC TGCTGCAGAT TGGACCAGCA GGGAAGGCTG ACCTGCCCCC CATCAATGGC 
CCGGTGGACG TGGACCGAGG TCCCATCATG CCCGTGCCCA TGGGCATCCG GCCCGTGCTC 
AGCAAGTACC GAGTGGAGGT GCTGTTCTGG GGCCTACGGG ACCTAAAGCG GGTGAACCTG 
GCCCAGGTGG ACCGGCCACG GGTGGACATC GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC 
CTGATCCACA ATTATAAGAA GAACCCCAAC TTCAACACCC TCGTCAAGTG GTTTGAAGTG 
GACCTCCCAG AGAACGAGCT GCTGCACCCG CCCTTGAACA TCCGTGTGGT GGACTGCCGG 
GCCTTCGGTC GCTACACACT GGTGGGCTCC CATGCCGTCA GCTCCCTGCG ACGCTTCATC 
TACCGGCCCC CAGACCGCTC GGCCCCCAGC TGGAACACCA CGGTCAGGCT TCTCCGGCGC 
TGCCGTGTGC TGTGCAATGG GGGCTCCTCC TCTCACTCCA CAGGGGAGGT TGTGGTGACT 
ATGGAGCCAG AGGTACCCAT CAAGAAACTG GAGACCATGG TGAAGCTGGA CGCGACTTCT 
GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG 
GGCACTGCGG AGGAGCCAGA GGAGGAGGAG CCAGACGAGA GCATGCTGGA CTGGTGGTCC 
AAGTACTTTG CCTCCATTGA CACCATGAAG GAGCAACTTC GACAACAAGA GCCCTCTGGA 
ATTGACTTGG AGGAGAAGGA GGAAGTGGAC AATACCGAGG GCCTGAAGGG GTCAATGAAG 
GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG GAGAAGAAGA AGAAAACTCA GAGCTCTGGC 
TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG AAGAAACCCA AGATTGATGA GCTTAAGGTA 
TACCCCAAAG AGCTGGAGTC CGAGTTTGAT AACTTTGAGG ACTGGCTGCA CACTTTCAAC 
TTGCTTCGGG GCAAGACCGG GGATGATGAG GATGGCTCCA CCGAGGAGGA GCGCATTGTG 
GGACGCTTCA AGGGCTCCCT CTGCGTGTAC AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG 
GAAGCCGGCT ACGACTCCAC CTACGGCATG TTCCAGGGCA TCCCGAGCAA TGACCCCATC 
AATGTGCTGG TCCGAGTCTA TGTGGTCCGG GCCACGGACC TGCACCCTGC TGACATCAAC 
GGCAAAGCTG ACCCCTACAT CGCCATCCGG CTAGGCAAGA CTGACATCCG CGACAAGGAG 
AACTACATCT CCAAGCAGCT CAACCCTGTC TTTGGGAAGT CCTTTGACAT CGAGGCCTCC 
TTCCCCATGG AATCCATGCT GACGGTGGCT GTGTATGACT GGGACCTGGT GGGCACTGAT 
GACCTCATTG GGGAAACCAA GATCGACCTG GAGAACCGCT TCTACAGCAA GCACCGCGCC 
ACCTGCGGCA TCGCCCAGAC CTACTCCACA CATGGCTACA ATATCTGGCG GGACCCCATG 
AAGCCCAGCC AGATCCTGAC CCGCCTCTGC AAAGACGGCA AAGTGGACGG CCCCCACTTT 
GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC CGCGTCTTCA CTGGGCCCTC TGAGATTGAG 
GACGAGAACG GTCAGAGGAA GCCCACAGAC GAGCATGTGG CGCTGTTGGC CCTGAGGCAC 
TGGGAGGACA TCCCCCGCGC AGGCTGCCGC CTGGTGCCAG AGCATGTGGA GACGAGGCCG 
CTGCTCAACC CCGACAAGCC GGGCATCGAG CAGGGCCGCC TGGAGCTGTG GGTGGACATG 
TTCCCCATGG ACATGCCAGC CCCTGGGACG CCTCTGGACA TCTCACCTCG GAAGCCCAAG 
AAGTACGAGC TGCGGGTCAT CATCTGGAAC ACAGATGAGG TGGTCTTGGA GGACGACGAC 
TTCTTCACAG GGGAGAAGTC CAGTGACATC TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG 
GAGGACAAGC AGGACACAGA CGTCCACTAC CACTCCCTCA CTGGCGAGGG CAACTTCAAC 
TGGCGCTACC TGTTCCCCTT CGACTACCTG GCGGCGGAGG AGAAGATCGT CATCTCCAAG 
AAGGAGTCCA TGTTCTCCTG GGACGAGACC GAGTACAAGA TCCCCGCGCG GCTCACCCTG 
CAGATCTGGG ATGCGGACCA CTTCTCCGCT GACGACTTCC TGGGGGCCAT CGAGCTGGAC 
CTGAACCGGT TCCCGCGGGG CGCAAAGACA GCCAAGCAGT GCACCATGGA GATGGCCACC 
GGGGAGGTGG ACGTGCCCCT CGTGTCCATC TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG 
CCCCTCCTGG CCCGCAATGA GAACGATGAG TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG 
CTGCATTTAC TGACAGCAGA GGAGGCAGAG AAGAACCCAG TGGGCCTGGC CCGCAATGAA 
CCTGACCCCC TAGAGAAACC CAACCGGCCC GACACGAGCT TCATCTGGTT CCTGAACCCT 
CTCAAGTCGG CTCGCTACTT CTTGTGGCAC ACGTATCGCT GGCTGCTCCT CAAACTGTTG 
CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC CTGTTCCTCT ACTCTGTGCC TGGCTACCTG 
GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919303.1
CDS	232..4155
Translation	MMTDTQDGPSESSQIMRSLTPLINREEAFGEAGEAGLWPSITHTPDSQEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	NM_194322.3
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194322.3

ORF Insert Sequence:

ATGATGACCG ATACTCAGGA TGGCCCTAGC GAGAGCTCCC AGATCATGAG GTCCCTCACT 
CCCCTGATCA ACAGGGAGGA GGCATTTGGG GAGGCTGGGG AGGCGGGGCT GTGGCCCAGC 
ATCACCCACA CTCCTGATTC ACAGGAAGAA GGCCTGAACG ACATACAGGA GATGATCAAA 
ACGGAGAAGT CCTACCCTGA GCGTCGCCTG CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC 
TGCTGCCGCT TCCTCTCCCT CGCTGACAAG GACCAGGGCC ACTCATCCCG CACCAGGCTT 
GACCGGGAGC GCCTCAAGTC CTGCATGAGG GAGCTGGAAA ACATGGGGCA GCAGGCCAGG 
ATGCTGCGGG CCCAGGTGAA GCGGCACACG GTGCGGGACA AGCTGAGGCT GTGCCAGAAC 
TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC GAGCCCCAGC ACAGCATTCC CGACATCTTC 
ATCTGGATGA TGAGCAACAA CAAGCGTGTC GCCTATGCCC GTGTGCCCTC CAAGGACCTG 
CTCTTCTCCA TCGTGGAGGA GGAGACTGGC AAGGACTGCG CCAAGGTCAA GACGCTCTTC 
CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC TCGGCAGGCT GGACAGTGCA GGCCAAGGTG 
GAGCTGTACC TGTGGCTGGG CCTCAGCAAA CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC 
TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG GGCCTGGGCC TGCATGCCTT CCCACCCGTC 
AGCCTGGTCT ACACCAAGAA GCAGGCGTTC CAGCTCCGAG CGCACATGTA CCAGGCCCGC 
AGCCTCTTTG CCGCCGACAG CAGCGGACTC TCAGACCCCT TTGCCCGCGT CTTCTTCATC 
AATCAGAGTC AGTGCACAGA GGTGCTGAAT GAGACCCTGT GTCCCACCTG GGACCAGATG 
CTGGTGTTCG ACAACCTGGA GCTCTATGGT GAAGCTCATG AGCTGAGGGA CGATCCGCCC 
ATCATTGTCA TTGAAATCTA TGACCAGGAT TCCATGGGCA AAGCTGACTT CATGGGCCGG 
ACCTTCGCCA AACCCCTGGT GAAGATGGCA GACGAGGCGT ACTGCCCACC CCGCTTCCCA 
CCTCAGCTCG AGTACTACCA GATCTACCGT GGCAACGCCA CAGCTGGAGA CCTGCTGGCG 
GCCTTCGAGC TGCTGCAGAT TGGACCAGCA GGGAAGGCTG ACCTGCCCCC CATCAATGGC 
CCGGTGGACG TGGACCGAGG TCCCATCATG CCCGTGCCCA TGGGCATCCG GCCCGTGCTC 
AGCAAGTACC GAGTGGAGGT GCTGTTCTGG GGCCTACGGG ACCTAAAGCG GGTGAACCTG 
GCCCAGGTGG ACCGGCCACG GGTGGACATC GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC 
CTGATCCACA ATTATAAGAA GAACCCCAAC TTCAACACCC TCGTCAAGTG GTTTGAAGTG 
GACCTCCCAG AGAACGAGCT GCTGCACCCG CCCTTGAACA TCCGTGTGGT GGACTGCCGG 
GCCTTCGGTC GCTACACACT GGTGGGCTCC CATGCCGTCA GCTCCCTGCG ACGCTTCATC 
TACCGGCCCC CAGACCGCTC GGCCCCCAGC TGGAACACCA CGGTCAGGCT TCTCCGGCGC 
TGCCGTGTGC TGTGCAATGG GGGCTCCTCC TCTCACTCCA CAGGGGAGGT TGTGGTGACT 
ATGGAGCCAG AGGTACCCAT CAAGAAACTG GAGACCATGG TGAAGCTGGA CGCGACTTCT 
GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG 
GGCACTGCGG AGGAGCCAGA GGAGGAGGAG CCAGACGAGA GCATGCTGGA CTGGTGGTCC 
AAGTACTTTG CCTCCATTGA CACCATGAAG GAGCAACTTC GACAACAAGA GCCCTCTGGA 
ATTGACTTGG AGGAGAAGGA GGAAGTGGAC AATACCGAGG GCCTGAAGGG GTCAATGAAG 
GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG GAGAAGAAGA AGAAAACTCA GAGCTCTGGC 
TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG AAGAAACCCA AGATTGATGA GCTTAAGGTA 
TACCCCAAAG AGCTGGAGTC CGAGTTTGAT AACTTTGAGG ACTGGCTGCA CACTTTCAAC 
TTGCTTCGGG GCAAGACCGG GGATGATGAG GATGGCTCCA CCGAGGAGGA GCGCATTGTG 
GGACGCTTCA AGGGCTCCCT CTGCGTGTAC AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG 
GAAGCCGGCT ACGACTCCAC CTACGGCATG TTCCAGGGCA TCCCGAGCAA TGACCCCATC 
AATGTGCTGG TCCGAGTCTA TGTGGTCCGG GCCACGGACC TGCACCCTGC TGACATCAAC 
GGCAAAGCTG ACCCCTACAT CGCCATCCGG CTAGGCAAGA CTGACATCCG CGACAAGGAG 
AACTACATCT CCAAGCAGCT CAACCCTGTC TTTGGGAAGT CCTTTGACAT CGAGGCCTCC 
TTCCCCATGG AATCCATGCT GACGGTGGCT GTGTATGACT GGGACCTGGT GGGCACTGAT 
GACCTCATTG GGGAAACCAA GATCGACCTG GAGAACCGCT TCTACAGCAA GCACCGCGCC 
ACCTGCGGCA TCGCCCAGAC CTACTCCACA CATGGCTACA ATATCTGGCG GGACCCCATG 
AAGCCCAGCC AGATCCTGAC CCGCCTCTGC AAAGACGGCA AAGTGGACGG CCCCCACTTT 
GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC CGCGTCTTCA CTGGGCCCTC TGAGATTGAG 
GACGAGAACG GTCAGAGGAA GCCCACAGAC GAGCATGTGG CGCTGTTGGC CCTGAGGCAC 
TGGGAGGACA TCCCCCGCGC AGGCTGCCGC CTGGTGCCAG AGCATGTGGA GACGAGGCCG 
CTGCTCAACC CCGACAAGCC GGGCATCGAG CAGGGCCGCC TGGAGCTGTG GGTGGACATG 
TTCCCCATGG ACATGCCAGC CCCTGGGACG CCTCTGGACA TCTCACCTCG GAAGCCCAAG 
AAGTACGAGC TGCGGGTCAT CATCTGGAAC ACAGATGAGG TGGTCTTGGA GGACGACGAC 
TTCTTCACAG GGGAGAAGTC CAGTGACATC TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG 
GAGGACAAGC AGGACACAGA CGTCCACTAC CACTCCCTCA CTGGCGAGGG CAACTTCAAC 
TGGCGCTACC TGTTCCCCTT CGACTACCTG GCGGCGGAGG AGAAGATCGT CATCTCCAAG 
AAGGAGTCCA TGTTCTCCTG GGACGAGACC GAGTACAAGA TCCCCGCGCG GCTCACCCTG 
CAGATCTGGG ATGCGGACCA CTTCTCCGCT GACGACTTCC TGGGGGCCAT CGAGCTGGAC 
CTGAACCGGT TCCCGCGGGG CGCAAAGACA GCCAAGCAGT GCACCATGGA GATGGCCACC 
GGGGAGGTGG ACGTGCCCCT CGTGTCCATC TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG 
CCCCTCCTGG CCCGCAATGA GAACGATGAG TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG 
CTGCATTTAC TGACAGCAGA GGAGGCAGAG AAGAACCCAG TGGGCCTGGC CCGCAATGAA 
CCTGACCCCC TAGAGAAACC CAACCGGCCC GACACGAGCT TCATCTGGTT CCTGAACCCT 
CTCAAGTCGG CTCGCTACTT CTTGTGGCAC ACGTATCGCT GGCTGCTCCT CAAACTGTTG 
CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC CTGTTCCTCT ACTCTGTGCC TGGCTACCTG 
GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_004802.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004793.2 Homo sapiens otoferlin (OTOF), transcript variant 2, mRNA.

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CloneID	OHu28598
Clone ID Related Accession (Same CDS sequence)	NM_004802.4 , NM_004802.3
Accession Version	NM_004802.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	otoferlin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093378.5, AF107403.1 and AF183187.1. On Jun 2, 2019 this sequence version replaced NM_004802.3. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b, also called 'short form 1') has a shorter N-terminus and lacks a segment compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF107403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGAGCTT CATCTGGTTC 
CTGAACCCTC TCAAGTCGGC TCGCTACTTC TTGTGGCACA CGTATCGCTG GCTGCTCCTC 
AAACTGTTGC TGCTCCTGCT GCTGCTCCTC CTCCTCGCCC TGTTCCTCTA CTCTGTGCCT 
GGCTACCTGG TCAAGAAAAT CCTCGGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004793.2
CDS	309..4001
Translation	MIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	NM_004802.4
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004802.4

ORF Insert Sequence:

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGAGCTT CATCTGGTTC 
CTGAACCCTC TCAAGTCGGC TCGCTACTTC TTGTGGCACA CGTATCGCTG GCTGCTCCTC 
AAACTGTTGC TGCTCCTGCT GCTGCTCCTC CTCCTCGCCC TGTTCCTCTA CTCTGTGCCT 
GGCTACCTGG TCAAGAAAAT CCTCGGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_004802.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004793.2 Homo sapiens otoferlin (OTOF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28598
Clone ID Related Accession (Same CDS sequence)	NM_004802.4 , NM_004802.3
Accession Version	NM_004802.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	otoferlin isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF107403.1, AC093378.5 and AF183187.1. On Dec 2, 2008 this sequence version replaced NM_004802.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b, also called 'short form 1') has a shorter N-terminus and lacks a segment compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF107403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGAGCTT CATCTGGTTC 
CTGAACCCTC TCAAGTCGGC TCGCTACTTC TTGTGGCACA CGTATCGCTG GCTGCTCCTC 
AAACTGTTGC TGCTCCTGCT GCTGCTCCTC CTCCTCGCCC TGTTCCTCTA CTCTGTGCCT 
GGCTACCTGG TCAAGAAAAT CCTCGGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004793.2
CDS	227..3919
Translation	MIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	NM_004802.3
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004802.3

ORF Insert Sequence:

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGAGCTT CATCTGGTTC 
CTGAACCCTC TCAAGTCGGC TCGCTACTTC TTGTGGCACA CGTATCGCTG GCTGCTCCTC 
AAACTGTTGC TGCTCCTGCT GCTGCTCCTC CTCCTCGCCC TGTTCCTCTA CTCTGTGCCT 
GGCTACCTGG TCAAGAAAAT CCTCGGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_194248.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_919224.1 Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28603
Clone ID Related Accession (Same CDS sequence)	NM_194248.2 , NM_194248.3
Accession Version	NM_194248.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	otoferlin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF183185.1, AC093378.5 and AF183187.1. This sequence is a reference standard in the RefSeqGene project. On Dec 2, 2008 this sequence version replaced NM_194248.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183185.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGAGCT TCATCTGGTT CCTGAACCCT CTCAAGTCGG CTCGCTACTT CTTGTGGCAC 
ACGTATCGCT GGCTGCTCCT CAAACTGTTG CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC 
CTGTTCCTCT ACTCTGTGCC TGGCTACCTG GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919224.1
CDS	128..6121
Translation	MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVEIVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDRRNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDLASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	NM_194248.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194248.2

ORF Insert Sequence:

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGAGCT TCATCTGGTT CCTGAACCCT CTCAAGTCGG CTCGCTACTT CTTGTGGCAC 
ACGTATCGCT GGCTGCTCCT CAAACTGTTG CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC 
CTGTTCCTCT ACTCTGTGCC TGGCTACCTG GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_194248.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_919224.1 Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.

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CloneID	OHu28603
Clone ID Related Accession (Same CDS sequence)	NM_194248.2 , NM_194248.3
Accession Version	NM_194248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	otoferlin isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108070.2, AF183185.1, AC093378.5 and AF183187.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_194248.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183185.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGAGCT TCATCTGGTT CCTGAACCCT CTCAAGTCGG CTCGCTACTT CTTGTGGCAC 
ACGTATCGCT GGCTGCTCCT CAAACTGTTG CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC 
CTGTTCCTCT ACTCTGTGCC TGGCTACCTG GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919224.1
CDS	186..6179
Translation	MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVEIVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDRRNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDLASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	NM_194248.3
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194248.3

ORF Insert Sequence:

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGAGCT TCATCTGGTT CCTGAACCCT CTCAAGTCGG CTCGCTACTT CTTGTGGCAC 
ACGTATCGCT GGCTGCTCCT CAAACTGTTG CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC 
CTGTTCCTCT ACTCTGTGCC TGGCTACCTG GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_001287489.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274418.1 Homo sapiens otoferlin (OTOF), transcript variant 5, mRNA.

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CloneID	OHu28605
Clone ID Related Accession (Same CDS sequence)	NM_001287489.2 , NM_001287489.1
Accession Version	NM_001287489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	otoferlin isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC108070.2, AF183185.1, AK296272.1, AC093378.5 and AF183187.1. On Jun 2, 2019 this sequence version replaced NM_001287489.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 3' coding region and uses a downstream stop codon compared to variant 1. It encodes isoform e which has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGGCCT TCGTCTGGTT CCTCAACCCT CTCAAGTCCA TCAAGTACCT CATCTGCACC 
CGGTACAAGT GGCTCATCAT CAAGATCGTG CTGGCGCTGT TGGGGCTGCT CATGTTGGGG 
CTCTTCCTCT ACAGCCTCCC TGGCTACATG GTCAAAAAGC TCCTTGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274418.1
CDS	186..6179
Translation	MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVEIVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDRRNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDLASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	NM_001287489.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287489.2

ORF Insert Sequence:

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGGCCT TCGTCTGGTT CCTCAACCCT CTCAAGTCCA TCAAGTACCT CATCTGCACC 
CGGTACAAGT GGCTCATCAT CAAGATCGTG CTGGCGCTGT TGGGGCTGCT CATGTTGGGG 
CTCTTCCTCT ACAGCCTCCC TGGCTACATG GTCAAAAAGC TCCTTGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_001287489.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274418.1 Homo sapiens otoferlin (OTOF), transcript variant 5, mRNA.

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CloneID	OHu28605
Clone ID Related Accession (Same CDS sequence)	NM_001287489.2 , NM_001287489.1
Accession Version	NM_001287489.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	otoferlin isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF183185.1, AK296272.1, AC093378.5 and AF183187.1. Transcript Variant: This variant (5) lacks an alternate in-frame exon in the 3' coding region and uses a downstream stop codon compared to variant 1. It encodes isoform e which has a distinct C-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGGCCT TCGTCTGGTT CCTCAACCCT CTCAAGTCCA TCAAGTACCT CATCTGCACC 
CGGTACAAGT GGCTCATCAT CAAGATCGTG CTGGCGCTGT TGGGGCTGCT CATGTTGGGG 
CTCTTCCTCT ACAGCCTCCC TGGCTACATG GTCAAAAAGC TCCTTGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274418.1
CDS	128..6121
Translation	MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVEIVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDRRNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDLASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	NM_001287489.1
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287489.1

ORF Insert Sequence:

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGTCAGGCT TCTCCGGCGC TGCCGTGTGC TGTGCAATGG GGGCTCCTCC 
TCTCACTCCA CAGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGGCCT TCGTCTGGTT CCTCAACCCT CTCAAGTCCA TCAAGTACCT CATCTGCACC 
CGGTACAAGT GGCTCATCAT CAAGATCGTG CTGGCGCTGT TGGGGCTGCT CATGTTGGGG 
CTCTTCCTCT ACAGCCTCCC TGGCTACATG GTCAAAAAGC TCCTTGGGGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_194323.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_919304.1 Homo sapiens otoferlin (OTOF), transcript variant 4, mRNA.

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CloneID	OHu28620
Clone ID Related Accession (Same CDS sequence)	NM_194323.3 , NM_194323.2
Accession Version	NM_194323.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	otoferlin isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC093378.5, AF183187.1 and AF183185.1. On Jun 2, 2019 this sequence version replaced NM_194323.2. Transcript Variant: This variant (4) differs in the 5' UTR and coding region, as well as in the 3' coding region, compared to variant 1. The resulting isoform (d) has a shorter N-terminus and a distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGGCCTT CGTCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAGTACCTC ATCTGCACCC GGTACAAGTG GCTCATCATC 
AAGATCGTGC TGGCGCTGTT GGGGCTGCTC ATGTTGGGGC TCTTCCTCTA CAGCCTCCCT 
GGCTACATGG TCAAAAAGCT CCTTGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919304.1
CDS	309..4001
Translation	MIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	NM_194323.3
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194323.3

ORF Insert Sequence:

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGGCCTT CGTCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAGTACCTC ATCTGCACCC GGTACAAGTG GCTCATCATC 
AAGATCGTGC TGGCGCTGTT GGGGCTGCTC ATGTTGGGGC TCTTCCTCTA CAGCCTCCCT 
GGCTACATGG TCAAAAAGCT CCTTGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( NM_194323.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_919304.1 Homo sapiens otoferlin (OTOF), transcript variant 4, mRNA.

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CloneID	OHu28620
Clone ID Related Accession (Same CDS sequence)	NM_194323.3 , NM_194323.2
Accession Version	NM_194323.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	otoferlin isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF183187.1, AF183185.1 and AC093378.5. On Dec 2, 2008 this sequence version replaced NM_194323.1. Transcript Variant: This variant (4) differs in the 5' UTR and coding region, as well as in the 3' coding region, compared to variant 1. The resulting isoform (d) has a shorter N-terminus and a distinct C-terminus compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF183187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGGCCTT CGTCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAGTACCTC ATCTGCACCC GGTACAAGTG GCTCATCATC 
AAGATCGTGC TGGCGCTGTT GGGGCTGCTC ATGTTGGGGC TCTTCCTCTA CAGCCTCCCT 
GGCTACATGG TCAAAAAGCT CCTTGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_919304.1
CDS	227..3919
Translation	MIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTAFVWFLNPLKSIKYLICTRYKWLIIKIVLALLGLLMLGLFLYSLPGYMVKKLLGA

Target ORF information:

RefSeq Version	NM_194323.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_194323.2

ORF Insert Sequence:

ATGATCAAAA CGGAGAAGTC CTACCCTGAG CGTCGCCTGC GGGGCGTCCT GGAGGAGCTG 
AGCTGTGGCT GCTGCCGCTT CCTCTCCCTC GCTGACAAGG ACCAGGGCCA CTCATCCCGC 
ACCAGGCTTG ACCGGGAGCG CCTCAAGTCC TGCATGAGGG AGCTGGAAAA CATGGGGCAG 
CAGGCCAGGA TGCTGCGGGC CCAGGTGAAG CGGCACACGG TGCGGGACAA GCTGAGGCTG 
TGCCAGAACT TCCTGCAGAA GCTGCGCTTC CTGGCGGACG AGCCCCAGCA CAGCATTCCC 
GACATCTTCA TCTGGATGAT GAGCAACAAC AAGCGTGTCG CCTATGCCCG TGTGCCCTCC 
AAGGACCTGC TCTTCTCCAT CGTGGAGGAG GAGACTGGCA AGGACTGCGC CAAGGTCAAG 
ACGCTCTTCC TTAAGCTGCC AGGGAAGCGG GGCTTCGGCT CGGCAGGCTG GACAGTGCAG 
GCCAAGGTGG AGCTGTACCT GTGGCTGGGC CTCAGCAAAC AGCGCAAGGA GTTCCTGTGC 
GGCCTGCCCT GTGGCTTCCA GGAGGTCAAG GCAGCCCAGG GCCTGGGCCT GCATGCCTTC 
CCACCCGTCA GCCTGGTCTA CACCAAGAAG CAGGCGTTCC AGCTCCGAGC GCACATGTAC 
CAGGCCCGCA GCCTCTTTGC CGCCGACAGC AGCGGACTCT CAGACCCCTT TGCCCGCGTC 
TTCTTCATCA ATCAGAGTCA GTGCACAGAG GTGCTGAATG AGACCCTGTG TCCCACCTGG 
GACCAGATGC TGGTGTTCGA CAACCTGGAG CTCTATGGTG AAGCTCATGA GCTGAGGGAC 
GATCCGCCCA TCATTGTCAT TGAAATCTAT GACCAGGATT CCATGGGCAA AGCTGACTTC 
ATGGGCCGGA CCTTCGCCAA ACCCCTGGTG AAGATGGCAG ACGAGGCGTA CTGCCCACCC 
CGCTTCCCAC CTCAGCTCGA GTACTACCAG ATCTACCGTG GCAACGCCAC AGCTGGAGAC 
CTGCTGGCGG CCTTCGAGCT GCTGCAGATT GGACCAGCAG GGAAGGCTGA CCTGCCCCCC 
ATCAATGGCC CGGTGGACGT GGACCGAGGT CCCATCATGC CCGTGCCCAT GGGCATCCGG 
CCCGTGCTCA GCAAGTACCG AGTGGAGGTG CTGTTCTGGG GCCTACGGGA CCTAAAGCGG 
GTGAACCTGG CCCAGGTGGA CCGGCCACGG GTGGACATCG AGTGTGCAGG GAAGGGGGTG 
CAGTCGTCCC TGATCCACAA TTATAAGAAG AACCCCAACT TCAACACCCT CGTCAAGTGG 
TTTGAAGTGG ACCTCCCAGA GAACGAGCTG CTGCACCCGC CCTTGAACAT CCGTGTGGTG 
GACTGCCGGG CCTTCGGTCG CTACACACTG GTGGGCTCCC ATGCCGTCAG CTCCCTGCGA 
CGCTTCATCT ACCGGCCCCC AGACCGCTCG GCCCCCAGCT GGAACACCAC GGGGGAGGTT 
GTGGTGACTA TGGAGCCAGA GGTACCCATC AAGAAACTGG AGACCATGGT GAAGCTGGAC 
GCGACTTCTG AAGCTGTTGT CAAGGTGGAT GTGGCTGAGG AGGAGAAGGA GAAGAAGAAG 
AAGAAGAAGG GCACTGCGGA GGAGCCAGAG GAGGAGGAGC CAGACGAGAG CATGCTGGAC 
TGGTGGTCCA AGTACTTTGC CTCCATTGAC ACCATGAAGG AGCAACTTCG ACAACAAGAG 
CCCTCTGGAA TTGACTTGGA GGAGAAGGAG GAAGTGGACA ATACCGAGGG CCTGAAGGGG 
TCAATGAAGG GCAAGGAGAA GGCAAGGGCT GCCAAAGAGG AGAAGAAGAA GAAAACTCAG 
AGCTCTGGCT CTGGCCAGGG GTCCGAGGCC CCCGAGAAGA AGAAACCCAA GATTGATGAG 
CTTAAGGTAT ACCCCAAAGA GCTGGAGTCC GAGTTTGATA ACTTTGAGGA CTGGCTGCAC 
ACTTTCAACT TGCTTCGGGG CAAGACCGGG GATGATGAGG ATGGCTCCAC CGAGGAGGAG 
CGCATTGTGG GACGCTTCAA GGGCTCCCTC TGCGTGTACA AAGTGCCACT CCCAGAGGAC 
GTGTCCCGGG AAGCCGGCTA CGACTCCACC TACGGCATGT TCCAGGGCAT CCCGAGCAAT 
GACCCCATCA ATGTGCTGGT CCGAGTCTAT GTGGTCCGGG CCACGGACCT GCACCCTGCT 
GACATCAACG GCAAAGCTGA CCCCTACATC GCCATCCGGC TAGGCAAGAC TGACATCCGC 
GACAAGGAGA ACTACATCTC CAAGCAGCTC AACCCTGTCT TTGGGAAGTC CTTTGACATC 
GAGGCCTCCT TCCCCATGGA ATCCATGCTG ACGGTGGCTG TGTATGACTG GGACCTGGTG 
GGCACTGATG ACCTCATTGG GGAAACCAAG ATCGACCTGG AGAACCGCTT CTACAGCAAG 
CACCGCGCCA CCTGCGGCAT CGCCCAGACC TACTCCACAC ATGGCTACAA TATCTGGCGG 
GACCCCATGA AGCCCAGCCA GATCCTGACC CGCCTCTGCA AAGACGGCAA AGTGGACGGC 
CCCCACTTTG GGCCCCCTGG GAGAGTGAAG GTGGCCAACC GCGTCTTCAC TGGGCCCTCT 
GAGATTGAGG ACGAGAACGG TCAGAGGAAG CCCACAGACG AGCATGTGGC GCTGTTGGCC 
CTGAGGCACT GGGAGGACAT CCCCCGCGCA GGCTGCCGCC TGGTGCCAGA GCATGTGGAG 
ACGAGGCCGC TGCTCAACCC CGACAAGCCG GGCATCGAGC AGGGCCGCCT GGAGCTGTGG 
GTGGACATGT TCCCCATGGA CATGCCAGCC CCTGGGACGC CTCTGGACAT CTCACCTCGG 
AAGCCCAAGA AGTACGAGCT GCGGGTCATC ATCTGGAACA CAGATGAGGT GGTCTTGGAG 
GACGACGACT TCTTCACAGG GGAGAAGTCC AGTGACATCT TCGTGAGGGG GTGGCTGAAG 
GGCCAGCAGG AGGACAAGCA GGACACAGAC GTCCACTACC ACTCCCTCAC TGGCGAGGGC 
AACTTCAACT GGCGCTACCT GTTCCCCTTC GACTACCTGG CGGCGGAGGA GAAGATCGTC 
ATCTCCAAGA AGGAGTCCAT GTTCTCCTGG GACGAGACCG AGTACAAGAT CCCCGCGCGG 
CTCACCCTGC AGATCTGGGA TGCGGACCAC TTCTCCGCTG ACGACTTCCT GGGGGCCATC 
GAGCTGGACC TGAACCGGTT CCCGCGGGGC GCAAAGACAG CCAAGCAGTG CACCATGGAG 
ATGGCCACCG GGGAGGTGGA CGTGCCCCTC GTGTCCATCT TCAAGCAAAA GCGCGTCAAA 
GGCTGGTGGC CCCTCCTGGC CCGCAATGAG AACGATGAGT TTGAGCTCAC GGGCAAGGTG 
GAGGCTGAGC TGCATTTACT GACAGCAGAG GAGGCAGAGA AGAACCCAGT GGGCCTGGCC 
CGCAATGAAC CTGACCCCCT AGAGAAACCC AACCGGCCCG ACACGGCCTT CGTCTGGTTC 
CTCAACCCTC TCAAGTCCAT CAAGTACCTC ATCTGCACCC GGTACAAGTG GCTCATCATC 
AAGATCGTGC TGGCGCTGTT GGGGCTGCTC ATGTTGGGGC TCTTCCTCTA CAGCCTCCCT 
GGCTACATGG TCAAAAAGCT CCTTGGGGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOF ( XM_017005338.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860827.1 Homo sapiens otoferlin (OTOF), transcript variant X1, mRNA.

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CloneID	OHu92767
Clone ID Related Accession (Same CDS sequence)	XM_017005338.1
Accession Version	XM_017005338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	otoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGAGCT TCATCTGGTT CCTGAACCCT CTCAAGTCGG CTCGCTACTT CTTGTGGCAC 
ACGTATCGCT GGCTGCTCCT CAAACTGTTG CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC 
CTGTTCCTCT ACTCTGTGCC TGGCTACCTG GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860827.1
CDS	1..5934
Translation	MALLIHLKTVSELRGRGDRIAKVTFRGQSFYSRVLENCEDVADFDETFRWPVASSIDRNEMLEIQVFNYSKVFSNKLIGTFRMVLQKVVEESHVEVTDTLIDDNNAIIKTSLCVEVRYQATDGTVGSWDDGDFLGDESLQEEEKDSQETDGLLPGSRPSSRPPGEKSFRRAGRSVFSAMKLGKNRSHKEEPQRPDEPAVLEMEDLDHLAIRLGDGLDPDSVSLASVTALTTNVSNKRSKPDIKMEPSAGRPMDYQVSITVIEARQLVGLNMDPVVCVEVGDDKKYTSMKESTNCPYYNEYFVFDFHVSPDVMFDKIIKISVIHSKNLLRSGTLVGSFKMDVGTVYSQPEHQFHHKWAILSDPDDISSGLKGYVKCDVAVVGKGDNIKTPHKANETDEDDIEGNLLLPEGVPPERQWARFYVKIYRAEGLPRMNTSLMANVKKAFIGENKDLVDPYVQVFFAGQKGKTSVQKSSYEPLWNEQVVFTDLFPPLCKRMKVQIRDSDKVNDVAIGTHFIDLRKISNDGDKGFLPTLGPAWVNMYGSTRNYTLLDEHQDLNEGLGEGVSFRARLLLGLAVEIVDTSNPELTSSTEVQVEQATPISESCAGKMEEFFLFGAFLEASMIDRRNGDKPITFEVTIGNYGNEVDGLSRPQRPRPRKEPGDEEEVDLIQNASDDEAGDAGDLASVSSTPPMRPQVTDRNYFHLPYLERKPCIYIKSWWPDQRRRLYNANIMDHIADKLEEGLNDIQEMIKTEKSYPERRLRGVLEELSCGCCRFLSLADKDQGHSSRTRLDRERLKSCMRELENMGQQARMLRAQVKRHTVRDKLRLCQNFLQKLRFLADEPQHSIPDIFIWMMSNNKRVAYARVPSKDLLFSIVEEETGKDCAKVKTLFLKLPGKRGFGSAGWTVQAKVELYLWLGLSKQRKEFLCGLPCGFQEVKAAQGLGLHAFPPVSLVYTKKQAFQLRAHMYQARSLFAADSSGLSDPFARVFFINQSQCTEVLNETLCPTWDQMLVFDNLELYGEAHELRDDPPIIVIEIYDQDSMGKADFMGRTFAKPLVKMADEAYCPPRFPPQLEYYQIYRGNATAGDLLAAFELLQIGPAGKADLPPINGPVDVDRGPIMPVPMGIRPVLSKYRVEVLFWGLRDLKRVNLAQVDRPRVDIECAGKGVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCRAFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTGEVVVTMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDWWSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQSSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEEERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLHPADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDWDLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDGKVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLVPEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNTDEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYLAAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAKTAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEEAEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLLLLALFLYSVPGYLVKKILGA

Target ORF information:

RefSeq Version	XM_017005338.1
Organism	Homo sapiens(human)
Definition	Homo sapiens otoferlin (OTOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005338.1

ORF Insert Sequence:

ATGGCCTTGC TCATCCACCT CAAGACAGTC TCGGAGCTGC GGGGCAGGGG CGACCGGATC 
GCCAAAGTGA CTTTCCGAGG GCAATCCTTC TACTCTCGGG TCCTGGAGAA CTGTGAGGAT 
GTGGCTGACT TTGATGAGAC ATTTCGGTGG CCGGTGGCCA GCAGCATCGA CAGAAATGAG 
ATGCTGGAGA TTCAGGTTTT CAACTACAGC AAAGTCTTCA GCAACAAGCT CATCGGGACC 
TTCCGCATGG TGCTGCAGAA GGTGGTAGAG GAGAGCCATG TGGAGGTGAC TGACACGCTG 
ATTGATGACA ACAATGCTAT CATCAAGACC AGCCTGTGCG TGGAGGTCCG GTATCAGGCC 
ACTGACGGCA CAGTGGGCTC CTGGGACGAT GGGGACTTCC TGGGAGATGA GTCTCTTCAA 
GAGGAAGAGA AGGACAGCCA AGAGACGGAT GGACTGCTCC CAGGCTCCCG GCCCAGCTCC 
CGGCCCCCAG GAGAGAAGAG CTTCCGGAGA GCCGGGAGGA GCGTGTTCTC CGCCATGAAG 
CTCGGCAAAA ACCGGTCTCA CAAGGAGGAG CCCCAAAGAC CAGATGAACC GGCGGTGCTG 
GAGATGGAAG ACCTTGACCA TCTGGCCATT CGGCTAGGAG ATGGACTGGA TCCCGACTCG 
GTGTCTCTAG CCTCAGTCAC AGCTCTCACC ACTAATGTCT CCAACAAGCG ATCTAAGCCA 
GACATTAAGA TGGAGCCAAG TGCTGGGCGG CCCATGGATT ACCAGGTCAG CATCACGGTG 
ATCGAGGCCC GGCAGCTGGT GGGCTTGAAC ATGGACCCTG TGGTGTGCGT GGAGGTGGGT 
GACGACAAGA AGTACACATC CATGAAGGAG TCCACTAACT GCCCCTATTA CAACGAGTAC 
TTCGTCTTCG ACTTCCATGT CTCTCCGGAT GTCATGTTTG ACAAGATCAT CAAGATTTCG 
GTGATTCACT CCAAGAACCT GCTGCGCAGT GGCACCCTGG TGGGCTCCTT CAAAATGGAC 
GTGGGAACCG TGTACTCGCA GCCAGAGCAC CAGTTCCATC ACAAGTGGGC CATCCTGTCT 
GACCCCGATG ACATCTCCTC GGGGCTGAAG GGCTACGTGA AGTGTGACGT TGCCGTGGTG 
GGCAAAGGGG ACAACATCAA GACGCCCCAC AAGGCCAATG AGACCGACGA AGATGACATT 
GAGGGGAACT TGCTGCTCCC CGAGGGGGTG CCCCCCGAAC GCCAGTGGGC CCGGTTCTAT 
GTGAAAATTT ACCGAGCAGA GGGGCTGCCC CGTATGAACA CAAGCCTCAT GGCCAATGTA 
AAGAAGGCTT TCATCGGTGA AAACAAGGAC CTCGTGGACC CCTACGTGCA AGTCTTCTTT 
GCTGGCCAGA AGGGCAAGAC TTCAGTGCAG AAGAGCAGCT ATGAGCCCCT GTGGAATGAG 
CAGGTCGTCT TTACAGACCT CTTCCCCCCA CTCTGCAAAC GCATGAAGGT GCAGATCCGA 
GACTCGGACA AGGTCAACGA CGTGGCCATC GGCACCCACT TCATTGACCT GCGCAAGATT 
TCTAATGACG GAGACAAAGG CTTCCTGCCC ACACTGGGCC CAGCCTGGGT GAACATGTAC 
GGCTCCACAC GTAACTACAC GCTGCTGGAT GAGCATCAGG ACCTGAACGA GGGCCTGGGG 
GAGGGTGTGT CCTTCCGGGC CCGGCTCCTG CTGGGCCTGG CTGTGGAGAT CGTAGACACC 
TCCAACCCTG AGCTCACCAG CTCCACAGAG GTGCAGGTGG AGCAGGCCAC GCCCATCTCG 
GAGAGCTGTG CAGGTAAAAT GGAAGAATTC TTTCTCTTTG GAGCCTTCCT GGAGGCCTCA 
ATGATCGACC GGAGAAACGG AGACAAGCCC ATCACCTTTG AGGTCACCAT AGGCAACTAT 
GGGAACGAAG TTGATGGCCT GTCCCGGCCC CAGCGGCCTC GGCCCCGGAA GGAGCCGGGG 
GATGAGGAAG AAGTAGACCT GATTCAGAAC GCAAGTGATG ACGAGGCCGG TGATGCCGGG 
GACCTGGCCT CAGTCTCCTC CACTCCACCA ATGCGGCCCC AGGTCACCGA CAGGAACTAC 
TTCCATCTGC CCTACCTGGA GCGAAAGCCC TGCATCTACA TCAAGAGCTG GTGGCCGGAC 
CAGCGCCGCC GCCTCTACAA TGCCAACATC ATGGACCACA TTGCCGACAA GCTGGAAGAA 
GGCCTGAACG ACATACAGGA GATGATCAAA ACGGAGAAGT CCTACCCTGA GCGTCGCCTG 
CGGGGCGTCC TGGAGGAGCT GAGCTGTGGC TGCTGCCGCT TCCTCTCCCT CGCTGACAAG 
GACCAGGGCC ACTCATCCCG CACCAGGCTT GACCGGGAGC GCCTCAAGTC CTGCATGAGG 
GAGCTGGAAA ACATGGGGCA GCAGGCCAGG ATGCTGCGGG CCCAGGTGAA GCGGCACACG 
GTGCGGGACA AGCTGAGGCT GTGCCAGAAC TTCCTGCAGA AGCTGCGCTT CCTGGCGGAC 
GAGCCCCAGC ACAGCATTCC CGACATCTTC ATCTGGATGA TGAGCAACAA CAAGCGTGTC 
GCCTATGCCC GTGTGCCCTC CAAGGACCTG CTCTTCTCCA TCGTGGAGGA GGAGACTGGC 
AAGGACTGCG CCAAGGTCAA GACGCTCTTC CTTAAGCTGC CAGGGAAGCG GGGCTTCGGC 
TCGGCAGGCT GGACAGTGCA GGCCAAGGTG GAGCTGTACC TGTGGCTGGG CCTCAGCAAA 
CAGCGCAAGG AGTTCCTGTG CGGCCTGCCC TGTGGCTTCC AGGAGGTCAA GGCAGCCCAG 
GGCCTGGGCC TGCATGCCTT CCCACCCGTC AGCCTGGTCT ACACCAAGAA GCAGGCGTTC 
CAGCTCCGAG CGCACATGTA CCAGGCCCGC AGCCTCTTTG CCGCCGACAG CAGCGGACTC 
TCAGACCCCT TTGCCCGCGT CTTCTTCATC AATCAGAGTC AGTGCACAGA GGTGCTGAAT 
GAGACCCTGT GTCCCACCTG GGACCAGATG CTGGTGTTCG ACAACCTGGA GCTCTATGGT 
GAAGCTCATG AGCTGAGGGA CGATCCGCCC ATCATTGTCA TTGAAATCTA TGACCAGGAT 
TCCATGGGCA AAGCTGACTT CATGGGCCGG ACCTTCGCCA AACCCCTGGT GAAGATGGCA 
GACGAGGCGT ACTGCCCACC CCGCTTCCCA CCTCAGCTCG AGTACTACCA GATCTACCGT 
GGCAACGCCA CAGCTGGAGA CCTGCTGGCG GCCTTCGAGC TGCTGCAGAT TGGACCAGCA 
GGGAAGGCTG ACCTGCCCCC CATCAATGGC CCGGTGGACG TGGACCGAGG TCCCATCATG 
CCCGTGCCCA TGGGCATCCG GCCCGTGCTC AGCAAGTACC GAGTGGAGGT GCTGTTCTGG 
GGCCTACGGG ACCTAAAGCG GGTGAACCTG GCCCAGGTGG ACCGGCCACG GGTGGACATC 
GAGTGTGCAG GGAAGGGGGT GCAGTCGTCC CTGATCCACA ATTATAAGAA GAACCCCAAC 
TTCAACACCC TCGTCAAGTG GTTTGAAGTG GACCTCCCAG AGAACGAGCT GCTGCACCCG 
CCCTTGAACA TCCGTGTGGT GGACTGCCGG GCCTTCGGTC GCTACACACT GGTGGGCTCC 
CATGCCGTCA GCTCCCTGCG ACGCTTCATC TACCGGCCCC CAGACCGCTC GGCCCCCAGC 
TGGAACACCA CGGGGGAGGT TGTGGTGACT ATGGAGCCAG AGGTACCCAT CAAGAAACTG 
GAGACCATGG TGAAGCTGGA CGCGACTTCT GAAGCTGTTG TCAAGGTGGA TGTGGCTGAG 
GAGGAGAAGG AGAAGAAGAA GAAGAAGAAG GGCACTGCGG AGGAGCCAGA GGAGGAGGAG 
CCAGACGAGA GCATGCTGGA CTGGTGGTCC AAGTACTTTG CCTCCATTGA CACCATGAAG 
GAGCAACTTC GACAACAAGA GCCCTCTGGA ATTGACTTGG AGGAGAAGGA GGAAGTGGAC 
AATACCGAGG GCCTGAAGGG GTCAATGAAG GGCAAGGAGA AGGCAAGGGC TGCCAAAGAG 
GAGAAGAAGA AGAAAACTCA GAGCTCTGGC TCTGGCCAGG GGTCCGAGGC CCCCGAGAAG 
AAGAAACCCA AGATTGATGA GCTTAAGGTA TACCCCAAAG AGCTGGAGTC CGAGTTTGAT 
AACTTTGAGG ACTGGCTGCA CACTTTCAAC TTGCTTCGGG GCAAGACCGG GGATGATGAG 
GATGGCTCCA CCGAGGAGGA GCGCATTGTG GGACGCTTCA AGGGCTCCCT CTGCGTGTAC 
AAAGTGCCAC TCCCAGAGGA CGTGTCCCGG GAAGCCGGCT ACGACTCCAC CTACGGCATG 
TTCCAGGGCA TCCCGAGCAA TGACCCCATC AATGTGCTGG TCCGAGTCTA TGTGGTCCGG 
GCCACGGACC TGCACCCTGC TGACATCAAC GGCAAAGCTG ACCCCTACAT CGCCATCCGG 
CTAGGCAAGA CTGACATCCG CGACAAGGAG AACTACATCT CCAAGCAGCT CAACCCTGTC 
TTTGGGAAGT CCTTTGACAT CGAGGCCTCC TTCCCCATGG AATCCATGCT GACGGTGGCT 
GTGTATGACT GGGACCTGGT GGGCACTGAT GACCTCATTG GGGAAACCAA GATCGACCTG 
GAGAACCGCT TCTACAGCAA GCACCGCGCC ACCTGCGGCA TCGCCCAGAC CTACTCCACA 
CATGGCTACA ATATCTGGCG GGACCCCATG AAGCCCAGCC AGATCCTGAC CCGCCTCTGC 
AAAGACGGCA AAGTGGACGG CCCCCACTTT GGGCCCCCTG GGAGAGTGAA GGTGGCCAAC 
CGCGTCTTCA CTGGGCCCTC TGAGATTGAG GACGAGAACG GTCAGAGGAA GCCCACAGAC 
GAGCATGTGG CGCTGTTGGC CCTGAGGCAC TGGGAGGACA TCCCCCGCGC AGGCTGCCGC 
CTGGTGCCAG AGCATGTGGA GACGAGGCCG CTGCTCAACC CCGACAAGCC GGGCATCGAG 
CAGGGCCGCC TGGAGCTGTG GGTGGACATG TTCCCCATGG ACATGCCAGC CCCTGGGACG 
CCTCTGGACA TCTCACCTCG GAAGCCCAAG AAGTACGAGC TGCGGGTCAT CATCTGGAAC 
ACAGATGAGG TGGTCTTGGA GGACGACGAC TTCTTCACAG GGGAGAAGTC CAGTGACATC 
TTCGTGAGGG GGTGGCTGAA GGGCCAGCAG GAGGACAAGC AGGACACAGA CGTCCACTAC 
CACTCCCTCA CTGGCGAGGG CAACTTCAAC TGGCGCTACC TGTTCCCCTT CGACTACCTG 
GCGGCGGAGG AGAAGATCGT CATCTCCAAG AAGGAGTCCA TGTTCTCCTG GGACGAGACC 
GAGTACAAGA TCCCCGCGCG GCTCACCCTG CAGATCTGGG ATGCGGACCA CTTCTCCGCT 
GACGACTTCC TGGGGGCCAT CGAGCTGGAC CTGAACCGGT TCCCGCGGGG CGCAAAGACA 
GCCAAGCAGT GCACCATGGA GATGGCCACC GGGGAGGTGG ACGTGCCCCT CGTGTCCATC 
TTCAAGCAAA AGCGCGTCAA AGGCTGGTGG CCCCTCCTGG CCCGCAATGA GAACGATGAG 
TTTGAGCTCA CGGGCAAGGT GGAGGCTGAG CTGCATTTAC TGACAGCAGA GGAGGCAGAG 
AAGAACCCAG TGGGCCTGGC CCGCAATGAA CCTGACCCCC TAGAGAAACC CAACCGGCCC 
GACACGAGCT TCATCTGGTT CCTGAACCCT CTCAAGTCGG CTCGCTACTT CTTGTGGCAC 
ACGTATCGCT GGCTGCTCCT CAAACTGTTG CTGCTCCTGC TGCTGCTCCT CCTCCTCGCC 
CTGTTCCTCT ACTCTGTGCC TGGCTACCTG GTCAAGAAAA TCCTCGGGGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	OTOF
Entrez Gene ID	9381
Full Name	otoferlin
Synonyms	AUNB1,DFNB6,DFNB9,FER1L2,NSRD9
General protein information	Preferred Names otoferlin Names otoferlin fer-1-like family member 2 fer-1-like protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.3
Summary	Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	603681
Disorder Html:	Deafness, autosomal recessive 9, 601071 (3); Auditory neuropathy, autosomal recessive, 1, 601071 (3)

Homo sapiens (human)	OTOF	NP_919224.1
Canis lupus familiaris (dog)	OTOF	XP_005630285.1
Bos taurus (cattle)	OTOF	NP_001137579.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000389	XP_310715.5
Xenopus tropicalis (tropical clawed frog)	otof	XP_004918245.1
Mus musculus (house mouse)	Otof	NP_114081.2
Gallus gallus (chicken)	OTOF	XP_420015.2
Danio rerio (zebrafish)	otof	NP_001025283.1
Pan troglodytes (chimpanzee)	OTOF	XP_003949828.1
	OTOF	XP_002799220.1
Rattus norvegicus (Norway rat)	Otof	NP_001263649.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

OTOF cDNA ORF clone, Homo sapiens(human)

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gRNAs

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